TOP3B cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP3B
Entrez Gene ID	477576
Full Name	DNA topoisomerase III beta
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014108221.2	XP_013963696.1	DNA topoisomerase 3-beta-1 isoform X3
XM_005636634.2	XP_005636691.1	DNA topoisomerase 3-beta-1 isoform X2
XM_014108221.1	XP_013963696.1	DNA topoisomerase 3-beta-1 isoform X3
XM_005636634.3	XP_005636691.1	DNA topoisomerase 3-beta-1 isoform X2
XM_534771.5	XP_534771.2	DNA topoisomerase 3-beta-1 isoform X1
XM_005636633.3	XP_005636690.1	DNA topoisomerase 3-beta-1 isoform X1
XM_005636633.2	XP_005636690.1	DNA topoisomerase 3-beta-1 isoform X1
XM_534771.6	XP_534771.2	DNA topoisomerase 3-beta-1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TOP3B	XP_515006.3
Rattus norvegicus (Norway rat)	Top3b	NP_001099331.1
Mus musculus (house mouse)	Top3b	NP_035754.1
Gallus gallus (chicken)	TOP3B	NP_001006181.1
Drosophila melanogaster (fruit fly)	Top3beta	NP_511059.2
Caenorhabditis elegans (roundworm)	Y48C3A.14	NP_001254381.1
Oryza sativa (rice)	Os09g0500600	NP_001063578.1
Homo sapiens (human)	TOP3B	NP_003926.1
Xenopus tropicalis (tropical clawed frog)	top3b	NP_001182501.1
	TOP3B	XP_001088462.2
Bos taurus (cattle)	TOP3B	NP_001193635.1
Danio rerio (zebrafish)	LOC793414	XP_003198651.1
Arabidopsis thaliana (thale cress)	AT2G32000	NP_180760.2
Canis lupus familiaris (dog)	TOP3B	XP_005636690.1

Gene Ontology

The following TOP3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe26759	XM_534771.6 Latest version!	Canis lupus familiaris DNA topoisomerase III beta (TOP3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe26759	XM_005636633.3 Latest version!	Canis lupus familiaris DNA topoisomerase III beta (TOP3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe26760	XM_005636634.3 Latest version!	Canis lupus familiaris DNA topoisomerase III beta (TOP3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe43496	XM_014108221.2 Latest version!	Canis lupus familiaris DNA topoisomerase III beta (TOP3B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OCe43496	XM_014108221.1	Canis lupus familiaris topoisomerase (DNA) III beta (TOP3B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe26760	XM_005636634.2	Canis lupus familiaris topoisomerase (DNA) III beta (TOP3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe26759	XM_005636633.2	Canis lupus familiaris topoisomerase (DNA) III beta (TOP3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe26759	XM_534771.5	Canis lupus familiaris topoisomerase (DNA) III beta (TOP3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP3B ( XM_534771.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_534771.2 Canis lupus familiaris topoisomerase (DNA) III beta (TOP3B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe26759
Clone ID Related Accession (Same CDS sequence)	XM_534771.5 , XM_005636633.3 , XM_005636633.2 , XM_534771.6
Accession Version	XM_534771.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	DNA topoisomerase 3-beta-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_534771.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACCG TGCTCATGGT TGCAGAAAAG CCATCCTTGG CCCAGTCCAT TGCTAAAATC 
CTCTCTCGGG GAAACATGTC CTCCCACAAA GGGCTGAACG GAACATGCTC CGTGCATGAG 
TACAGCGGGA CCTTTGCTGG CCAGCCCGTC CGCTTCAAGA TGACATCCGT CTGTGGTCAT 
GTGATGACCT TGGATTTCCT GGGAAAGTAC AACAAATGGG ACAAGGTGGA CCCCGCAGAG 
CTGTTCAGCC AAGCCCCGAC AGAGAAGAAG GAGGCTAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAA AGGCTGCGAC TATATTGTGC TATGGCTGGA CTGCGACAAG 
GAGGGAGAGA ACATCTGTTT TGAGGTCCTG GACGCTGTGC TGCCCGTCAT GAATCCAACC 
CATAGTGGCG AAAAGACCGT GTTCCGGGCC AGGTTCAGCT CCATCACAGA CACGGACATC 
TGTGCTGCCA TGGCCCGCCT GGGCGAGCCA GACCACAACG AGGCGCTGTC GGTGGACGCC 
AGGCAAGAGC TGGACCTGCG CATCGGCTGT GCGTTCACCA GATTTCAAAC TAAGTACTTC 
CAGGGAAAAT ATGGCAATCT AGACAGCTCC CTCATCTCCT TTGGGCCATG TCAAACCCCT 
ACCCTGGGCT TCTGCGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC GGAGACGTAC 
TGGGTGCTGC AGGCCAAGGT CAATGCCGAT AAAGACAGAT CTCTTCTTTT GGACTGGGAC 
CGAGTACGAG TGTTCGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG 
AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAGAAGG CCAAGCAGAG GCCCCTCGCC 
CTTAACACTG TGGAGATGCT GCGTGTCGCT AGCTCTTCTC TGGGCATGGG GCCACAGCAT 
GCCATGCAGA CCGCTGAGCG CCTCTACACG CAAGGCTACA TCAGCTACCC GAGGACTGAG 
ACTACCCACT ACCCCGAGAA CTTTGACCTG AAGGGCCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CCGACACGGT AAAGCGCTTG TTAGCCGAAG GTATCAACCG GCCACGGAAA 
GGCCATGATG CCGGCGACCA TCCTCCCATC ACCCCCATGA GATCTGCCAC AGAGGCTGAA 
TTAGGGAGTG AGGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACTGTT 
AGCTATGACT GCAAGTACCT CCAGAGCACC ATTTCCTTCA GAATCGGGCC TGAGCACTTC 
ACGTGCACGG GGAAAACCGT CATCTCTCCA GGCTTCACGG AGATCATGCC TTGGCAGAGT 
GTGCCTCTGG AGGAGAGCCT GCCCACCTGC CAGAGGGGCG ACTGCTTTCC TGTGAGCGAG 
ATGAAGATGC TAGAGAAGCA GACCAGCCCG CCCGACTACC TGACGGAGGC TGAGCTCATC 
ACACTCATGG AGAAGCACGG CATTGGGACA GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT CACTGTGGAG AGCGGGCGCC GCCTCAAACC CACCAACCTG 
GGCATCGTCC TGGTGCACGG CTACTACAAG ATTGATGCAG AGCTCGTGCT CCCCACTATC 
CGCAGCGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCTGA CTACCGCCAG 
GTCCTGGGCC ACACGCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTTGT GGACTCCATC 
GCCGGGATGG ACGAGCTGAT GGAGGTGTCT TTTTCACCCC TGGCAGCCAC GGGCAAGCCC 
CTCTCCCGCT GTGGAAAGTG CCACCGGTTC ATGAAGTACA TTCAGGCCAA GCCAAGCCGC 
CTACACTGCT CACACTGTGA CGAGACCTAC ACCCTCCCCC AGAATGGCAC CATCAAGCTC 
TACAAGGAGC TCCGGTGTCC ACTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCGGGCTCT 
CGGGGCAAGA GCTACCCACT GTGCCCCTAT TGCTCCAACC ACCCACCCTT CCGAGACATG 
AAGAAAGGCA TGGGCTGCAA TGAGTGCACA CACCCCACCT GCCAGCACTC ACTGAGCATG 
CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGCGTGC TGGTGCTGGA CCCCACCTCA 
GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TGGCACACTG CTTTGAGAAT 
GCCTACCGTG TGCGGGTGTC TGCCGACACC TGCCCCACCT GTGAGGCTGC CCTGCTTGCC 
GTGGACTTCA ACAAGGCCAA ATCCCCGCTC CCAGGTGACG AGACACAGCA CACAGGCTGC 
GTCTTCTGCG ACCCTGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GGGGAGGGCC AGGGAGGAGG CAGGGCCGAG GGCGGGCTCG GGGCCGGCGG 
CCCACAGGGA AGCCCAGTGC CAGGCGGCCG AAGGACAAGA TGTCAGCCCT GGCTGCCTAC 
TTTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_534771.2
CDS	158..2746
Translation	MKTVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGTCSVHEYSGTFAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGKGCDYIVLWLDCDKEGENICFEVLDAVLPVMNPTHSGEKTVFRARFSSITDTDICAAMARLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNADKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGPLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMRSATEAELGSEAWRLYEYITRHFIATVSYDCKYLQSTISFRIGPEHFTCTGKTVISPGFTEIMPWQSVPLEESLPTCQRGDCFPVSEMKMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCSNHPPFRDMKKGMGCNECTHPTCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAYRVRVSADTCPTCEAALLAVDFNKAKSPLPGDETQHTGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRARGRRPTGKPSARRPKDKMSALAAYFV

Target ORF information:

RefSeq Version	XM_534771.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris topoisomerase (DNA) III beta (TOP3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_534771.5

ORF Insert Sequence:

ATGAAGACCG TGCTCATGGT TGCAGAAAAG CCATCCTTGG CCCAGTCCAT TGCTAAAATC 
CTCTCTCGGG GAAACATGTC CTCCCACAAA GGGCTGAACG GAACATGCTC CGTGCATGAG 
TACAGCGGGA CCTTTGCTGG CCAGCCCGTC CGCTTCAAGA TGACATCCGT CTGTGGTCAT 
GTGATGACCT TGGATTTCCT GGGAAAGTAC AACAAATGGG ACAAGGTGGA CCCCGCAGAG 
CTGTTCAGCC AAGCCCCGAC AGAGAAGAAG GAGGCTAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAA AGGCTGCGAC TATATTGTGC TATGGCTGGA CTGCGACAAG 
GAGGGAGAGA ACATCTGTTT TGAGGTCCTG GACGCTGTGC TGCCCGTCAT GAATCCAACC 
CATAGTGGCG AAAAGACCGT GTTCCGGGCC AGGTTCAGCT CCATCACAGA CACGGACATC 
TGTGCTGCCA TGGCCCGCCT GGGCGAGCCA GACCACAACG AGGCGCTGTC GGTGGACGCC 
AGGCAAGAGC TGGACCTGCG CATCGGCTGT GCGTTCACCA GATTTCAAAC TAAGTACTTC 
CAGGGAAAAT ATGGCAATCT AGACAGCTCC CTCATCTCCT TTGGGCCATG TCAAACCCCT 
ACCCTGGGCT TCTGCGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC GGAGACGTAC 
TGGGTGCTGC AGGCCAAGGT CAATGCCGAT AAAGACAGAT CTCTTCTTTT GGACTGGGAC 
CGAGTACGAG TGTTCGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG 
AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAGAAGG CCAAGCAGAG GCCCCTCGCC 
CTTAACACTG TGGAGATGCT GCGTGTCGCT AGCTCTTCTC TGGGCATGGG GCCACAGCAT 
GCCATGCAGA CCGCTGAGCG CCTCTACACG CAAGGCTACA TCAGCTACCC GAGGACTGAG 
ACTACCCACT ACCCCGAGAA CTTTGACCTG AAGGGCCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CCGACACGGT AAAGCGCTTG TTAGCCGAAG GTATCAACCG GCCACGGAAA 
GGCCATGATG CCGGCGACCA TCCTCCCATC ACCCCCATGA GATCTGCCAC AGAGGCTGAA 
TTAGGGAGTG AGGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACTGTT 
AGCTATGACT GCAAGTACCT CCAGAGCACC ATTTCCTTCA GAATCGGGCC TGAGCACTTC 
ACGTGCACGG GGAAAACCGT CATCTCTCCA GGCTTCACGG AGATCATGCC TTGGCAGAGT 
GTGCCTCTGG AGGAGAGCCT GCCCACCTGC CAGAGGGGCG ACTGCTTTCC TGTGAGCGAG 
ATGAAGATGC TAGAGAAGCA GACCAGCCCG CCCGACTACC TGACGGAGGC TGAGCTCATC 
ACACTCATGG AGAAGCACGG CATTGGGACA GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT CACTGTGGAG AGCGGGCGCC GCCTCAAACC CACCAACCTG 
GGCATCGTCC TGGTGCACGG CTACTACAAG ATTGATGCAG AGCTCGTGCT CCCCACTATC 
CGCAGCGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCTGA CTACCGCCAG 
GTCCTGGGCC ACACGCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTTGT GGACTCCATC 
GCCGGGATGG ACGAGCTGAT GGAGGTGTCT TTTTCACCCC TGGCAGCCAC GGGCAAGCCC 
CTCTCCCGCT GTGGAAAGTG CCACCGGTTC ATGAAGTACA TTCAGGCCAA GCCAAGCCGC 
CTACACTGCT CACACTGTGA CGAGACCTAC ACCCTCCCCC AGAATGGCAC CATCAAGCTC 
TACAAGGAGC TCCGGTGTCC ACTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCGGGCTCT 
CGGGGCAAGA GCTACCCACT GTGCCCCTAT TGCTCCAACC ACCCACCCTT CCGAGACATG 
AAGAAAGGCA TGGGCTGCAA TGAGTGCACA CACCCCACCT GCCAGCACTC ACTGAGCATG 
CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGCGTGC TGGTGCTGGA CCCCACCTCA 
GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TGGCACACTG CTTTGAGAAT 
GCCTACCGTG TGCGGGTGTC TGCCGACACC TGCCCCACCT GTGAGGCTGC CCTGCTTGCC 
GTGGACTTCA ACAAGGCCAA ATCCCCGCTC CCAGGTGACG AGACACAGCA CACAGGCTGC 
GTCTTCTGCG ACCCTGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GGGGAGGGCC AGGGAGGAGG CAGGGCCGAG GGCGGGCTCG GGGCCGGCGG 
CCCACAGGGA AGCCCAGTGC CAGGCGGCCG AAGGACAAGA TGTCAGCCCT GGCTGCCTAC 
TTTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3B ( XM_005636633.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005636690.1 Canis lupus familiaris DNA topoisomerase III beta (TOP3B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe26759
Clone ID Related Accession (Same CDS sequence)	XM_534771.5 , XM_005636633.3 , XM_005636633.2 , XM_534771.6
Accession Version	XM_005636633.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DNA topoisomerase 3-beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636633.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACCG TGCTCATGGT TGCAGAAAAG CCATCCTTGG CCCAGTCCAT TGCTAAAATC 
CTCTCTCGGG GAAACATGTC CTCCCACAAA GGGCTGAACG GAACATGCTC CGTGCATGAG 
TACAGCGGGA CCTTTGCTGG CCAGCCCGTC CGCTTCAAGA TGACATCCGT CTGTGGTCAT 
GTGATGACCT TGGATTTCCT GGGAAAGTAC AACAAATGGG ACAAGGTGGA CCCCGCAGAG 
CTGTTCAGCC AAGCCCCGAC AGAGAAGAAG GAGGCTAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAA AGGCTGCGAC TATATTGTGC TATGGCTGGA CTGCGACAAG 
GAGGGAGAGA ACATCTGTTT TGAGGTCCTG GACGCTGTGC TGCCCGTCAT GAATCCAACC 
CATAGTGGCG AAAAGACCGT GTTCCGGGCC AGGTTCAGCT CCATCACAGA CACGGACATC 
TGTGCTGCCA TGGCCCGCCT GGGCGAGCCA GACCACAACG AGGCGCTGTC GGTGGACGCC 
AGGCAAGAGC TGGACCTGCG CATCGGCTGT GCGTTCACCA GATTTCAAAC TAAGTACTTC 
CAGGGAAAAT ATGGCAATCT AGACAGCTCC CTCATCTCCT TTGGGCCATG TCAAACCCCT 
ACCCTGGGCT TCTGCGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC GGAGACGTAC 
TGGGTGCTGC AGGCCAAGGT CAATGCCGAT AAAGACAGAT CTCTTCTTTT GGACTGGGAC 
CGAGTACGAG TGTTCGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG 
AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAGAAGG CCAAGCAGAG GCCCCTCGCC 
CTTAACACTG TGGAGATGCT GCGTGTCGCT AGCTCTTCTC TGGGCATGGG GCCACAGCAT 
GCCATGCAGA CCGCTGAGCG CCTCTACACG CAAGGCTACA TCAGCTACCC GAGGACTGAG 
ACTACCCACT ACCCCGAGAA CTTTGACCTG AAGGGCCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CCGACACGGT AAAGCGCTTG TTAGCCGAAG GTATCAACCG GCCACGGAAA 
GGCCATGATG CCGGCGACCA TCCTCCCATC ACCCCCATGA GATCTGCCAC AGAGGCTGAA 
TTAGGGAGTG AGGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACTGTT 
AGCTATGACT GCAAGTACCT CCAGAGCACC ATTTCCTTCA GAATCGGGCC TGAGCACTTC 
ACGTGCACGG GGAAAACCGT CATCTCTCCA GGCTTCACGG AGATCATGCC TTGGCAGAGT 
GTGCCTCTGG AGGAGAGCCT GCCCACCTGC CAGAGGGGCG ACTGCTTTCC TGTGAGCGAG 
ATGAAGATGC TAGAGAAGCA GACCAGCCCG CCCGACTACC TGACGGAGGC TGAGCTCATC 
ACACTCATGG AGAAGCACGG CATTGGGACA GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT CACTGTGGAG AGCGGGCGCC GCCTCAAACC CACCAACCTG 
GGCATCGTCC TGGTGCACGG CTACTACAAG ATTGATGCAG AGCTCGTGCT CCCCACTATC 
CGCAGCGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCTGA CTACCGCCAG 
GTCCTGGGCC ACACGCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTTGT GGACTCCATC 
GCCGGGATGG ACGAGCTGAT GGAGGTGTCT TTTTCACCCC TGGCAGCCAC GGGCAAGCCC 
CTCTCCCGCT GTGGAAAGTG CCACCGGTTC ATGAAGTACA TTCAGGCCAA GCCAAGCCGC 
CTACACTGCT CACACTGTGA CGAGACCTAC ACCCTCCCCC AGAATGGCAC CATCAAGCTC 
TACAAGGAGC TCCGGTGTCC ACTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCGGGCTCT 
CGGGGCAAGA GCTACCCACT GTGCCCCTAT TGCTCCAACC ACCCACCCTT CCGAGACATG 
AAGAAAGGCA TGGGCTGCAA TGAGTGCACA CACCCCACCT GCCAGCACTC ACTGAGCATG 
CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGCGTGC TGGTGCTGGA CCCCACCTCA 
GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TGGCACACTG CTTTGAGAAT 
GCCTACCGTG TGCGGGTGTC TGCCGACACC TGCCCCACCT GTGAGGCTGC CCTGCTTGCC 
GTGGACTTCA ACAAGGCCAA ATCCCCGCTC CCAGGTGACG AGACACAGCA CACAGGCTGC 
GTCTTCTGCG ACCCTGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GGGGAGGGCC AGGGAGGAGG CAGGGCCGAG GGCGGGCTCG GGGCCGGCGG 
CCCACAGGGA AGCCCAGTGC CAGGCGGCCG AAGGACAAGA TGTCAGCCCT GGCTGCCTAC 
TTTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005636690.1
CDS	753..3341
Translation	MKTVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGTCSVHEYSGTFAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGKGCDYIVLWLDCDKEGENICFEVLDAVLPVMNPTHSGEKTVFRARFSSITDTDICAAMARLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNADKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGPLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMRSATEAELGSEAWRLYEYITRHFIATVSYDCKYLQSTISFRIGPEHFTCTGKTVISPGFTEIMPWQSVPLEESLPTCQRGDCFPVSEMKMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCSNHPPFRDMKKGMGCNECTHPTCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAYRVRVSADTCPTCEAALLAVDFNKAKSPLPGDETQHTGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRARGRRPTGKPSARRPKDKMSALAAYFV

Target ORF information:

RefSeq Version	XM_005636633.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DNA topoisomerase III beta (TOP3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005636633.3

ORF Insert Sequence:

ATGAAGACCG TGCTCATGGT TGCAGAAAAG CCATCCTTGG CCCAGTCCAT TGCTAAAATC 
CTCTCTCGGG GAAACATGTC CTCCCACAAA GGGCTGAACG GAACATGCTC CGTGCATGAG 
TACAGCGGGA CCTTTGCTGG CCAGCCCGTC CGCTTCAAGA TGACATCCGT CTGTGGTCAT 
GTGATGACCT TGGATTTCCT GGGAAAGTAC AACAAATGGG ACAAGGTGGA CCCCGCAGAG 
CTGTTCAGCC AAGCCCCGAC AGAGAAGAAG GAGGCTAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAA AGGCTGCGAC TATATTGTGC TATGGCTGGA CTGCGACAAG 
GAGGGAGAGA ACATCTGTTT TGAGGTCCTG GACGCTGTGC TGCCCGTCAT GAATCCAACC 
CATAGTGGCG AAAAGACCGT GTTCCGGGCC AGGTTCAGCT CCATCACAGA CACGGACATC 
TGTGCTGCCA TGGCCCGCCT GGGCGAGCCA GACCACAACG AGGCGCTGTC GGTGGACGCC 
AGGCAAGAGC TGGACCTGCG CATCGGCTGT GCGTTCACCA GATTTCAAAC TAAGTACTTC 
CAGGGAAAAT ATGGCAATCT AGACAGCTCC CTCATCTCCT TTGGGCCATG TCAAACCCCT 
ACCCTGGGCT TCTGCGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC GGAGACGTAC 
TGGGTGCTGC AGGCCAAGGT CAATGCCGAT AAAGACAGAT CTCTTCTTTT GGACTGGGAC 
CGAGTACGAG TGTTCGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG 
AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAGAAGG CCAAGCAGAG GCCCCTCGCC 
CTTAACACTG TGGAGATGCT GCGTGTCGCT AGCTCTTCTC TGGGCATGGG GCCACAGCAT 
GCCATGCAGA CCGCTGAGCG CCTCTACACG CAAGGCTACA TCAGCTACCC GAGGACTGAG 
ACTACCCACT ACCCCGAGAA CTTTGACCTG AAGGGCCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CCGACACGGT AAAGCGCTTG TTAGCCGAAG GTATCAACCG GCCACGGAAA 
GGCCATGATG CCGGCGACCA TCCTCCCATC ACCCCCATGA GATCTGCCAC AGAGGCTGAA 
TTAGGGAGTG AGGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACTGTT 
AGCTATGACT GCAAGTACCT CCAGAGCACC ATTTCCTTCA GAATCGGGCC TGAGCACTTC 
ACGTGCACGG GGAAAACCGT CATCTCTCCA GGCTTCACGG AGATCATGCC TTGGCAGAGT 
GTGCCTCTGG AGGAGAGCCT GCCCACCTGC CAGAGGGGCG ACTGCTTTCC TGTGAGCGAG 
ATGAAGATGC TAGAGAAGCA GACCAGCCCG CCCGACTACC TGACGGAGGC TGAGCTCATC 
ACACTCATGG AGAAGCACGG CATTGGGACA GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT CACTGTGGAG AGCGGGCGCC GCCTCAAACC CACCAACCTG 
GGCATCGTCC TGGTGCACGG CTACTACAAG ATTGATGCAG AGCTCGTGCT CCCCACTATC 
CGCAGCGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCTGA CTACCGCCAG 
GTCCTGGGCC ACACGCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTTGT GGACTCCATC 
GCCGGGATGG ACGAGCTGAT GGAGGTGTCT TTTTCACCCC TGGCAGCCAC GGGCAAGCCC 
CTCTCCCGCT GTGGAAAGTG CCACCGGTTC ATGAAGTACA TTCAGGCCAA GCCAAGCCGC 
CTACACTGCT CACACTGTGA CGAGACCTAC ACCCTCCCCC AGAATGGCAC CATCAAGCTC 
TACAAGGAGC TCCGGTGTCC ACTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCGGGCTCT 
CGGGGCAAGA GCTACCCACT GTGCCCCTAT TGCTCCAACC ACCCACCCTT CCGAGACATG 
AAGAAAGGCA TGGGCTGCAA TGAGTGCACA CACCCCACCT GCCAGCACTC ACTGAGCATG 
CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGCGTGC TGGTGCTGGA CCCCACCTCA 
GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TGGCACACTG CTTTGAGAAT 
GCCTACCGTG TGCGGGTGTC TGCCGACACC TGCCCCACCT GTGAGGCTGC CCTGCTTGCC 
GTGGACTTCA ACAAGGCCAA ATCCCCGCTC CCAGGTGACG AGACACAGCA CACAGGCTGC 
GTCTTCTGCG ACCCTGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GGGGAGGGCC AGGGAGGAGG CAGGGCCGAG GGCGGGCTCG GGGCCGGCGG 
CCCACAGGGA AGCCCAGTGC CAGGCGGCCG AAGGACAAGA TGTCAGCCCT GGCTGCCTAC 
TTTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3B ( XM_005636633.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005636690.1 Canis lupus familiaris topoisomerase (DNA) III beta (TOP3B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe26759
Clone ID Related Accession (Same CDS sequence)	XM_534771.5 , XM_005636633.3 , XM_005636633.2 , XM_534771.6
Accession Version	XM_005636633.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	DNA topoisomerase 3-beta-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636633.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACCG TGCTCATGGT TGCAGAAAAG CCATCCTTGG CCCAGTCCAT TGCTAAAATC 
CTCTCTCGGG GAAACATGTC CTCCCACAAA GGGCTGAACG GAACATGCTC CGTGCATGAG 
TACAGCGGGA CCTTTGCTGG CCAGCCCGTC CGCTTCAAGA TGACATCCGT CTGTGGTCAT 
GTGATGACCT TGGATTTCCT GGGAAAGTAC AACAAATGGG ACAAGGTGGA CCCCGCAGAG 
CTGTTCAGCC AAGCCCCGAC AGAGAAGAAG GAGGCTAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAA AGGCTGCGAC TATATTGTGC TATGGCTGGA CTGCGACAAG 
GAGGGAGAGA ACATCTGTTT TGAGGTCCTG GACGCTGTGC TGCCCGTCAT GAATCCAACC 
CATAGTGGCG AAAAGACCGT GTTCCGGGCC AGGTTCAGCT CCATCACAGA CACGGACATC 
TGTGCTGCCA TGGCCCGCCT GGGCGAGCCA GACCACAACG AGGCGCTGTC GGTGGACGCC 
AGGCAAGAGC TGGACCTGCG CATCGGCTGT GCGTTCACCA GATTTCAAAC TAAGTACTTC 
CAGGGAAAAT ATGGCAATCT AGACAGCTCC CTCATCTCCT TTGGGCCATG TCAAACCCCT 
ACCCTGGGCT TCTGCGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC GGAGACGTAC 
TGGGTGCTGC AGGCCAAGGT CAATGCCGAT AAAGACAGAT CTCTTCTTTT GGACTGGGAC 
CGAGTACGAG TGTTCGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG 
AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAGAAGG CCAAGCAGAG GCCCCTCGCC 
CTTAACACTG TGGAGATGCT GCGTGTCGCT AGCTCTTCTC TGGGCATGGG GCCACAGCAT 
GCCATGCAGA CCGCTGAGCG CCTCTACACG CAAGGCTACA TCAGCTACCC GAGGACTGAG 
ACTACCCACT ACCCCGAGAA CTTTGACCTG AAGGGCCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CCGACACGGT AAAGCGCTTG TTAGCCGAAG GTATCAACCG GCCACGGAAA 
GGCCATGATG CCGGCGACCA TCCTCCCATC ACCCCCATGA GATCTGCCAC AGAGGCTGAA 
TTAGGGAGTG AGGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACTGTT 
AGCTATGACT GCAAGTACCT CCAGAGCACC ATTTCCTTCA GAATCGGGCC TGAGCACTTC 
ACGTGCACGG GGAAAACCGT CATCTCTCCA GGCTTCACGG AGATCATGCC TTGGCAGAGT 
GTGCCTCTGG AGGAGAGCCT GCCCACCTGC CAGAGGGGCG ACTGCTTTCC TGTGAGCGAG 
ATGAAGATGC TAGAGAAGCA GACCAGCCCG CCCGACTACC TGACGGAGGC TGAGCTCATC 
ACACTCATGG AGAAGCACGG CATTGGGACA GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT CACTGTGGAG AGCGGGCGCC GCCTCAAACC CACCAACCTG 
GGCATCGTCC TGGTGCACGG CTACTACAAG ATTGATGCAG AGCTCGTGCT CCCCACTATC 
CGCAGCGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCTGA CTACCGCCAG 
GTCCTGGGCC ACACGCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTTGT GGACTCCATC 
GCCGGGATGG ACGAGCTGAT GGAGGTGTCT TTTTCACCCC TGGCAGCCAC GGGCAAGCCC 
CTCTCCCGCT GTGGAAAGTG CCACCGGTTC ATGAAGTACA TTCAGGCCAA GCCAAGCCGC 
CTACACTGCT CACACTGTGA CGAGACCTAC ACCCTCCCCC AGAATGGCAC CATCAAGCTC 
TACAAGGAGC TCCGGTGTCC ACTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCGGGCTCT 
CGGGGCAAGA GCTACCCACT GTGCCCCTAT TGCTCCAACC ACCCACCCTT CCGAGACATG 
AAGAAAGGCA TGGGCTGCAA TGAGTGCACA CACCCCACCT GCCAGCACTC ACTGAGCATG 
CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGCGTGC TGGTGCTGGA CCCCACCTCA 
GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TGGCACACTG CTTTGAGAAT 
GCCTACCGTG TGCGGGTGTC TGCCGACACC TGCCCCACCT GTGAGGCTGC CCTGCTTGCC 
GTGGACTTCA ACAAGGCCAA ATCCCCGCTC CCAGGTGACG AGACACAGCA CACAGGCTGC 
GTCTTCTGCG ACCCTGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GGGGAGGGCC AGGGAGGAGG CAGGGCCGAG GGCGGGCTCG GGGCCGGCGG 
CCCACAGGGA AGCCCAGTGC CAGGCGGCCG AAGGACAAGA TGTCAGCCCT GGCTGCCTAC 
TTTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005636690.1
CDS	895..3483
Translation	MKTVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGTCSVHEYSGTFAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGKGCDYIVLWLDCDKEGENICFEVLDAVLPVMNPTHSGEKTVFRARFSSITDTDICAAMARLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNADKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGPLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMRSATEAELGSEAWRLYEYITRHFIATVSYDCKYLQSTISFRIGPEHFTCTGKTVISPGFTEIMPWQSVPLEESLPTCQRGDCFPVSEMKMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCSNHPPFRDMKKGMGCNECTHPTCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAYRVRVSADTCPTCEAALLAVDFNKAKSPLPGDETQHTGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRARGRRPTGKPSARRPKDKMSALAAYFV

Target ORF information:

RefSeq Version	XM_005636633.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris topoisomerase (DNA) III beta (TOP3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005636633.2

ORF Insert Sequence:

ATGAAGACCG TGCTCATGGT TGCAGAAAAG CCATCCTTGG CCCAGTCCAT TGCTAAAATC 
CTCTCTCGGG GAAACATGTC CTCCCACAAA GGGCTGAACG GAACATGCTC CGTGCATGAG 
TACAGCGGGA CCTTTGCTGG CCAGCCCGTC CGCTTCAAGA TGACATCCGT CTGTGGTCAT 
GTGATGACCT TGGATTTCCT GGGAAAGTAC AACAAATGGG ACAAGGTGGA CCCCGCAGAG 
CTGTTCAGCC AAGCCCCGAC AGAGAAGAAG GAGGCTAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAA AGGCTGCGAC TATATTGTGC TATGGCTGGA CTGCGACAAG 
GAGGGAGAGA ACATCTGTTT TGAGGTCCTG GACGCTGTGC TGCCCGTCAT GAATCCAACC 
CATAGTGGCG AAAAGACCGT GTTCCGGGCC AGGTTCAGCT CCATCACAGA CACGGACATC 
TGTGCTGCCA TGGCCCGCCT GGGCGAGCCA GACCACAACG AGGCGCTGTC GGTGGACGCC 
AGGCAAGAGC TGGACCTGCG CATCGGCTGT GCGTTCACCA GATTTCAAAC TAAGTACTTC 
CAGGGAAAAT ATGGCAATCT AGACAGCTCC CTCATCTCCT TTGGGCCATG TCAAACCCCT 
ACCCTGGGCT TCTGCGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC GGAGACGTAC 
TGGGTGCTGC AGGCCAAGGT CAATGCCGAT AAAGACAGAT CTCTTCTTTT GGACTGGGAC 
CGAGTACGAG TGTTCGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG 
AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAGAAGG CCAAGCAGAG GCCCCTCGCC 
CTTAACACTG TGGAGATGCT GCGTGTCGCT AGCTCTTCTC TGGGCATGGG GCCACAGCAT 
GCCATGCAGA CCGCTGAGCG CCTCTACACG CAAGGCTACA TCAGCTACCC GAGGACTGAG 
ACTACCCACT ACCCCGAGAA CTTTGACCTG AAGGGCCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CCGACACGGT AAAGCGCTTG TTAGCCGAAG GTATCAACCG GCCACGGAAA 
GGCCATGATG CCGGCGACCA TCCTCCCATC ACCCCCATGA GATCTGCCAC AGAGGCTGAA 
TTAGGGAGTG AGGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACTGTT 
AGCTATGACT GCAAGTACCT CCAGAGCACC ATTTCCTTCA GAATCGGGCC TGAGCACTTC 
ACGTGCACGG GGAAAACCGT CATCTCTCCA GGCTTCACGG AGATCATGCC TTGGCAGAGT 
GTGCCTCTGG AGGAGAGCCT GCCCACCTGC CAGAGGGGCG ACTGCTTTCC TGTGAGCGAG 
ATGAAGATGC TAGAGAAGCA GACCAGCCCG CCCGACTACC TGACGGAGGC TGAGCTCATC 
ACACTCATGG AGAAGCACGG CATTGGGACA GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT CACTGTGGAG AGCGGGCGCC GCCTCAAACC CACCAACCTG 
GGCATCGTCC TGGTGCACGG CTACTACAAG ATTGATGCAG AGCTCGTGCT CCCCACTATC 
CGCAGCGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCTGA CTACCGCCAG 
GTCCTGGGCC ACACGCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTTGT GGACTCCATC 
GCCGGGATGG ACGAGCTGAT GGAGGTGTCT TTTTCACCCC TGGCAGCCAC GGGCAAGCCC 
CTCTCCCGCT GTGGAAAGTG CCACCGGTTC ATGAAGTACA TTCAGGCCAA GCCAAGCCGC 
CTACACTGCT CACACTGTGA CGAGACCTAC ACCCTCCCCC AGAATGGCAC CATCAAGCTC 
TACAAGGAGC TCCGGTGTCC ACTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCGGGCTCT 
CGGGGCAAGA GCTACCCACT GTGCCCCTAT TGCTCCAACC ACCCACCCTT CCGAGACATG 
AAGAAAGGCA TGGGCTGCAA TGAGTGCACA CACCCCACCT GCCAGCACTC ACTGAGCATG 
CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGCGTGC TGGTGCTGGA CCCCACCTCA 
GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TGGCACACTG CTTTGAGAAT 
GCCTACCGTG TGCGGGTGTC TGCCGACACC TGCCCCACCT GTGAGGCTGC CCTGCTTGCC 
GTGGACTTCA ACAAGGCCAA ATCCCCGCTC CCAGGTGACG AGACACAGCA CACAGGCTGC 
GTCTTCTGCG ACCCTGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GGGGAGGGCC AGGGAGGAGG CAGGGCCGAG GGCGGGCTCG GGGCCGGCGG 
CCCACAGGGA AGCCCAGTGC CAGGCGGCCG AAGGACAAGA TGTCAGCCCT GGCTGCCTAC 
TTTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3B ( XM_534771.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_534771.2 Canis lupus familiaris DNA topoisomerase III beta (TOP3B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe26759
Clone ID Related Accession (Same CDS sequence)	XM_534771.5 , XM_005636633.3 , XM_005636633.2 , XM_534771.6
Accession Version	XM_534771.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DNA topoisomerase 3-beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_534771.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACCG TGCTCATGGT TGCAGAAAAG CCATCCTTGG CCCAGTCCAT TGCTAAAATC 
CTCTCTCGGG GAAACATGTC CTCCCACAAA GGGCTGAACG GAACATGCTC CGTGCATGAG 
TACAGCGGGA CCTTTGCTGG CCAGCCCGTC CGCTTCAAGA TGACATCCGT CTGTGGTCAT 
GTGATGACCT TGGATTTCCT GGGAAAGTAC AACAAATGGG ACAAGGTGGA CCCCGCAGAG 
CTGTTCAGCC AAGCCCCGAC AGAGAAGAAG GAGGCTAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAA AGGCTGCGAC TATATTGTGC TATGGCTGGA CTGCGACAAG 
GAGGGAGAGA ACATCTGTTT TGAGGTCCTG GACGCTGTGC TGCCCGTCAT GAATCCAACC 
CATAGTGGCG AAAAGACCGT GTTCCGGGCC AGGTTCAGCT CCATCACAGA CACGGACATC 
TGTGCTGCCA TGGCCCGCCT GGGCGAGCCA GACCACAACG AGGCGCTGTC GGTGGACGCC 
AGGCAAGAGC TGGACCTGCG CATCGGCTGT GCGTTCACCA GATTTCAAAC TAAGTACTTC 
CAGGGAAAAT ATGGCAATCT AGACAGCTCC CTCATCTCCT TTGGGCCATG TCAAACCCCT 
ACCCTGGGCT TCTGCGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC GGAGACGTAC 
TGGGTGCTGC AGGCCAAGGT CAATGCCGAT AAAGACAGAT CTCTTCTTTT GGACTGGGAC 
CGAGTACGAG TGTTCGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG 
AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAGAAGG CCAAGCAGAG GCCCCTCGCC 
CTTAACACTG TGGAGATGCT GCGTGTCGCT AGCTCTTCTC TGGGCATGGG GCCACAGCAT 
GCCATGCAGA CCGCTGAGCG CCTCTACACG CAAGGCTACA TCAGCTACCC GAGGACTGAG 
ACTACCCACT ACCCCGAGAA CTTTGACCTG AAGGGCCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CCGACACGGT AAAGCGCTTG TTAGCCGAAG GTATCAACCG GCCACGGAAA 
GGCCATGATG CCGGCGACCA TCCTCCCATC ACCCCCATGA GATCTGCCAC AGAGGCTGAA 
TTAGGGAGTG AGGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACTGTT 
AGCTATGACT GCAAGTACCT CCAGAGCACC ATTTCCTTCA GAATCGGGCC TGAGCACTTC 
ACGTGCACGG GGAAAACCGT CATCTCTCCA GGCTTCACGG AGATCATGCC TTGGCAGAGT 
GTGCCTCTGG AGGAGAGCCT GCCCACCTGC CAGAGGGGCG ACTGCTTTCC TGTGAGCGAG 
ATGAAGATGC TAGAGAAGCA GACCAGCCCG CCCGACTACC TGACGGAGGC TGAGCTCATC 
ACACTCATGG AGAAGCACGG CATTGGGACA GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT CACTGTGGAG AGCGGGCGCC GCCTCAAACC CACCAACCTG 
GGCATCGTCC TGGTGCACGG CTACTACAAG ATTGATGCAG AGCTCGTGCT CCCCACTATC 
CGCAGCGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCTGA CTACCGCCAG 
GTCCTGGGCC ACACGCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTTGT GGACTCCATC 
GCCGGGATGG ACGAGCTGAT GGAGGTGTCT TTTTCACCCC TGGCAGCCAC GGGCAAGCCC 
CTCTCCCGCT GTGGAAAGTG CCACCGGTTC ATGAAGTACA TTCAGGCCAA GCCAAGCCGC 
CTACACTGCT CACACTGTGA CGAGACCTAC ACCCTCCCCC AGAATGGCAC CATCAAGCTC 
TACAAGGAGC TCCGGTGTCC ACTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCGGGCTCT 
CGGGGCAAGA GCTACCCACT GTGCCCCTAT TGCTCCAACC ACCCACCCTT CCGAGACATG 
AAGAAAGGCA TGGGCTGCAA TGAGTGCACA CACCCCACCT GCCAGCACTC ACTGAGCATG 
CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGCGTGC TGGTGCTGGA CCCCACCTCA 
GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TGGCACACTG CTTTGAGAAT 
GCCTACCGTG TGCGGGTGTC TGCCGACACC TGCCCCACCT GTGAGGCTGC CCTGCTTGCC 
GTGGACTTCA ACAAGGCCAA ATCCCCGCTC CCAGGTGACG AGACACAGCA CACAGGCTGC 
GTCTTCTGCG ACCCTGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GGGGAGGGCC AGGGAGGAGG CAGGGCCGAG GGCGGGCTCG GGGCCGGCGG 
CCCACAGGGA AGCCCAGTGC CAGGCGGCCG AAGGACAAGA TGTCAGCCCT GGCTGCCTAC 
TTTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_534771.2
CDS	200..2788
Translation	MKTVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGTCSVHEYSGTFAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGKGCDYIVLWLDCDKEGENICFEVLDAVLPVMNPTHSGEKTVFRARFSSITDTDICAAMARLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNADKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGPLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMRSATEAELGSEAWRLYEYITRHFIATVSYDCKYLQSTISFRIGPEHFTCTGKTVISPGFTEIMPWQSVPLEESLPTCQRGDCFPVSEMKMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCSNHPPFRDMKKGMGCNECTHPTCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAYRVRVSADTCPTCEAALLAVDFNKAKSPLPGDETQHTGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRARGRRPTGKPSARRPKDKMSALAAYFV

Target ORF information:

RefSeq Version	XM_534771.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DNA topoisomerase III beta (TOP3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_534771.6

ORF Insert Sequence:

ATGAAGACCG TGCTCATGGT TGCAGAAAAG CCATCCTTGG CCCAGTCCAT TGCTAAAATC 
CTCTCTCGGG GAAACATGTC CTCCCACAAA GGGCTGAACG GAACATGCTC CGTGCATGAG 
TACAGCGGGA CCTTTGCTGG CCAGCCCGTC CGCTTCAAGA TGACATCCGT CTGTGGTCAT 
GTGATGACCT TGGATTTCCT GGGAAAGTAC AACAAATGGG ACAAGGTGGA CCCCGCAGAG 
CTGTTCAGCC AAGCCCCGAC AGAGAAGAAG GAGGCTAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAA AGGCTGCGAC TATATTGTGC TATGGCTGGA CTGCGACAAG 
GAGGGAGAGA ACATCTGTTT TGAGGTCCTG GACGCTGTGC TGCCCGTCAT GAATCCAACC 
CATAGTGGCG AAAAGACCGT GTTCCGGGCC AGGTTCAGCT CCATCACAGA CACGGACATC 
TGTGCTGCCA TGGCCCGCCT GGGCGAGCCA GACCACAACG AGGCGCTGTC GGTGGACGCC 
AGGCAAGAGC TGGACCTGCG CATCGGCTGT GCGTTCACCA GATTTCAAAC TAAGTACTTC 
CAGGGAAAAT ATGGCAATCT AGACAGCTCC CTCATCTCCT TTGGGCCATG TCAAACCCCT 
ACCCTGGGCT TCTGCGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC GGAGACGTAC 
TGGGTGCTGC AGGCCAAGGT CAATGCCGAT AAAGACAGAT CTCTTCTTTT GGACTGGGAC 
CGAGTACGAG TGTTCGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG 
AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAGAAGG CCAAGCAGAG GCCCCTCGCC 
CTTAACACTG TGGAGATGCT GCGTGTCGCT AGCTCTTCTC TGGGCATGGG GCCACAGCAT 
GCCATGCAGA CCGCTGAGCG CCTCTACACG CAAGGCTACA TCAGCTACCC GAGGACTGAG 
ACTACCCACT ACCCCGAGAA CTTTGACCTG AAGGGCCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CCGACACGGT AAAGCGCTTG TTAGCCGAAG GTATCAACCG GCCACGGAAA 
GGCCATGATG CCGGCGACCA TCCTCCCATC ACCCCCATGA GATCTGCCAC AGAGGCTGAA 
TTAGGGAGTG AGGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACTGTT 
AGCTATGACT GCAAGTACCT CCAGAGCACC ATTTCCTTCA GAATCGGGCC TGAGCACTTC 
ACGTGCACGG GGAAAACCGT CATCTCTCCA GGCTTCACGG AGATCATGCC TTGGCAGAGT 
GTGCCTCTGG AGGAGAGCCT GCCCACCTGC CAGAGGGGCG ACTGCTTTCC TGTGAGCGAG 
ATGAAGATGC TAGAGAAGCA GACCAGCCCG CCCGACTACC TGACGGAGGC TGAGCTCATC 
ACACTCATGG AGAAGCACGG CATTGGGACA GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT CACTGTGGAG AGCGGGCGCC GCCTCAAACC CACCAACCTG 
GGCATCGTCC TGGTGCACGG CTACTACAAG ATTGATGCAG AGCTCGTGCT CCCCACTATC 
CGCAGCGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCTGA CTACCGCCAG 
GTCCTGGGCC ACACGCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTTGT GGACTCCATC 
GCCGGGATGG ACGAGCTGAT GGAGGTGTCT TTTTCACCCC TGGCAGCCAC GGGCAAGCCC 
CTCTCCCGCT GTGGAAAGTG CCACCGGTTC ATGAAGTACA TTCAGGCCAA GCCAAGCCGC 
CTACACTGCT CACACTGTGA CGAGACCTAC ACCCTCCCCC AGAATGGCAC CATCAAGCTC 
TACAAGGAGC TCCGGTGTCC ACTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCGGGCTCT 
CGGGGCAAGA GCTACCCACT GTGCCCCTAT TGCTCCAACC ACCCACCCTT CCGAGACATG 
AAGAAAGGCA TGGGCTGCAA TGAGTGCACA CACCCCACCT GCCAGCACTC ACTGAGCATG 
CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGCGTGC TGGTGCTGGA CCCCACCTCA 
GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TGGCACACTG CTTTGAGAAT 
GCCTACCGTG TGCGGGTGTC TGCCGACACC TGCCCCACCT GTGAGGCTGC CCTGCTTGCC 
GTGGACTTCA ACAAGGCCAA ATCCCCGCTC CCAGGTGACG AGACACAGCA CACAGGCTGC 
GTCTTCTGCG ACCCTGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GGGGAGGGCC AGGGAGGAGG CAGGGCCGAG GGCGGGCTCG GGGCCGGCGG 
CCCACAGGGA AGCCCAGTGC CAGGCGGCCG AAGGACAAGA TGTCAGCCCT GGCTGCCTAC 
TTTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3B ( XM_005636634.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005636691.1 Canis lupus familiaris topoisomerase (DNA) III beta (TOP3B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe26760
Clone ID Related Accession (Same CDS sequence)	XM_005636634.2 , XM_005636634.3
Accession Version	XM_005636634.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	DNA topoisomerase 3-beta-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636634.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACCG TGCTCATGGT TGCAGAAAAG CCATCCTTGG CCCAGTCCAT TGCTAAAATC 
CTCTCTCGGG GAAACATGTC CTCCCACAAA GGGCTGAACG GAACATGCTC CGTGCATGAG 
TACAGCGGGA CCTTTGCTGG CCAGCCCGTC CGCTTCAAGA TGACATCCGT CTGTGGTCAT 
GTGATGACCT TGGATTTCCT GGGAAAGTAC AACAAATGGG ACAAGGTGGA CCCCGCAGAG 
CTGTTCAGCC AAGCCCCGAC AGAGAAGAAG GAGGCTAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAA AGGCTGCGAC TATATTGTGC TATGGCTGGA CTGCGACAAG 
GAGGGAGAGA ACATCTGTTT TGAGGTCCTG GACGCTGTGC TGCCCGTCAT GAATCCAACC 
CATAGTGGCG AAAAGACCGT GTTCCGGGCC AGGTTCAGCT CCATCACAGA CACGGACATC 
TGTGCTGCCA TGGCCCGCCT GGGCGAGCCA GACCACAACG AGGCGCTGTC GGTGGACGCC 
AGGCAAGAGC TGGACCTGCG CATCGGCTGT GCGTTCACCA GATTTCAAAC TAAGTACTTC 
CAGGGAAAAT ATGGCAATCT AGACAGCTCC CTCATCTCCT TTGGGCCATG TCAAACCCCT 
ACCCTGGGCT TCTGCGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC GGAGACGTAC 
TGGGTGCTGC AGGCCAAGGT CAATGCCGAT AAAGACAGAT CTCTTCTTTT GGACTGGGAC 
CGAGTACGAG TGTTCGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG 
AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAGAAGG CCAAGCAGAG GCCCCTCGCC 
CTTAACACTG TGGAGATGCT GCGTGTCGCT AGCTCTTCTC TGGGCATGGG GCCACAGCAT 
GCCATGCAGA CCGCTGAGCG CCTCTACACG CAAGGCTACA TCAGCTACCC GAGGACTGAG 
ACTACCCACT ACCCCGAGAA CTTTGACCTG AAGGGCCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CCGACACGGT AAAGCGCTTG TTAGCCGAAG GTATCAACCG GCCACGGAAA 
GGCCATGATG CCGGCGACCA TCCTCCCATC ACCCCCATGA GATCTGCCAC AGAGGCTGAA 
TTAGGGAGTG AGGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACTGTT 
AGCTATGACT GCAAGTACCT CCAGAGCACC ATTTCCTTCA GAATCGGGCC TGAGCACTTC 
ACGTGCACGG GGAAAACCGT CATCTCTCCA GGCTTCACGG AGATCATGCC TTGGCAGAGT 
GTGCCTCTGG AGGAGAGCCT GCCCACCTGC CAGAGGGGCG ACTGCTTTCC TGTGAGCGAG 
ATGAAGATGC TAGAGAAGCA GACCAGCCCG CCCGACTACC TGACGGAGGC TGAGCTCATC 
ACACTCATGG AGAAGCACGG CATTGGGACA GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT CACTGTGGAG AGCGGGCGCC GCCTCAAACC CACCAACCTG 
GGCATCGTCC TGGTGCACGG CTACTACAAG ATTGATGCAG AGCTCGTGCT CCCCACTATC 
CGCAGCGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCTGA CTACCGCCAG 
GTCCTGGGCC ACACGCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTTGT GGACTCCATC 
GCCGCCACGG GCAAGCCCCT CTCCCGCTGT GGAAAGTGCC ACCGGTTCAT GAAGTACATT 
CAGGCCAAGC CAAGCCGCCT ACACTGCTCA CACTGTGACG AGACCTACAC CCTCCCCCAG 
AATGGCACCA TCAAGCTCTA CAAGGAGCTC CGGTGTCCAC TGGATGACTT CGAGCTGGTC 
CTGTGGTCAT CGGGCTCTCG GGGCAAGAGC TACCCACTGT GCCCCTATTG CTCCAACCAC 
CCACCCTTCC GAGACATGAA GAAAGGCATG GGCTGCAATG AGTGCACACA CCCCACCTGC 
CAGCACTCAC TGAGCATGCT GGGCATCGGC CAGTGCGTGG AATGTGAGAG CGGCGTGCTG 
GTGCTGGACC CCACCTCAGG CCCCAAGTGG AAGGTGGCCT GCAACAAGTG CAACGTGGTG 
GCACACTGCT TTGAGAATGC CTACCGTGTG CGGGTGTCTG CCGACACCTG CCCCACCTGT 
GAGGCTGCCC TGCTTGCCGT GGACTTCAAC AAGGCCAAAT CCCCGCTCCC AGGTGACGAG 
ACACAGCACA CAGGCTGCGT CTTCTGCGAC CCTGTCTTCC AGGAGCTGGT GGAGCTGAAG 
CACGCAGCCT CCTGCCACCC CATGCACCGG GGAGGGCCAG GGAGGAGGCA GGGCCGAGGG 
CGGGCTCGGG GCCGGCGGCC CACAGGGAAG CCCAGTGCCA GGCGGCCGAA GGACAAGATG 
TCAGCCCTGG CTGCCTACTT TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005636691.1
CDS	895..3441
Translation	MKTVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGTCSVHEYSGTFAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGKGCDYIVLWLDCDKEGENICFEVLDAVLPVMNPTHSGEKTVFRARFSSITDTDICAAMARLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNADKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGPLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMRSATEAELGSEAWRLYEYITRHFIATVSYDCKYLQSTISFRIGPEHFTCTGKTVISPGFTEIMPWQSVPLEESLPTCQRGDCFPVSEMKMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCSNHPPFRDMKKGMGCNECTHPTCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAYRVRVSADTCPTCEAALLAVDFNKAKSPLPGDETQHTGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRARGRRPTGKPSARRPKDKMSALAAYFV

Target ORF information:

RefSeq Version	XM_005636634.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris topoisomerase (DNA) III beta (TOP3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005636634.2

ORF Insert Sequence:

ATGAAGACCG TGCTCATGGT TGCAGAAAAG CCATCCTTGG CCCAGTCCAT TGCTAAAATC 
CTCTCTCGGG GAAACATGTC CTCCCACAAA GGGCTGAACG GAACATGCTC CGTGCATGAG 
TACAGCGGGA CCTTTGCTGG CCAGCCCGTC CGCTTCAAGA TGACATCCGT CTGTGGTCAT 
GTGATGACCT TGGATTTCCT GGGAAAGTAC AACAAATGGG ACAAGGTGGA CCCCGCAGAG 
CTGTTCAGCC AAGCCCCGAC AGAGAAGAAG GAGGCTAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAA AGGCTGCGAC TATATTGTGC TATGGCTGGA CTGCGACAAG 
GAGGGAGAGA ACATCTGTTT TGAGGTCCTG GACGCTGTGC TGCCCGTCAT GAATCCAACC 
CATAGTGGCG AAAAGACCGT GTTCCGGGCC AGGTTCAGCT CCATCACAGA CACGGACATC 
TGTGCTGCCA TGGCCCGCCT GGGCGAGCCA GACCACAACG AGGCGCTGTC GGTGGACGCC 
AGGCAAGAGC TGGACCTGCG CATCGGCTGT GCGTTCACCA GATTTCAAAC TAAGTACTTC 
CAGGGAAAAT ATGGCAATCT AGACAGCTCC CTCATCTCCT TTGGGCCATG TCAAACCCCT 
ACCCTGGGCT TCTGCGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC GGAGACGTAC 
TGGGTGCTGC AGGCCAAGGT CAATGCCGAT AAAGACAGAT CTCTTCTTTT GGACTGGGAC 
CGAGTACGAG TGTTCGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG 
AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAGAAGG CCAAGCAGAG GCCCCTCGCC 
CTTAACACTG TGGAGATGCT GCGTGTCGCT AGCTCTTCTC TGGGCATGGG GCCACAGCAT 
GCCATGCAGA CCGCTGAGCG CCTCTACACG CAAGGCTACA TCAGCTACCC GAGGACTGAG 
ACTACCCACT ACCCCGAGAA CTTTGACCTG AAGGGCCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CCGACACGGT AAAGCGCTTG TTAGCCGAAG GTATCAACCG GCCACGGAAA 
GGCCATGATG CCGGCGACCA TCCTCCCATC ACCCCCATGA GATCTGCCAC AGAGGCTGAA 
TTAGGGAGTG AGGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACTGTT 
AGCTATGACT GCAAGTACCT CCAGAGCACC ATTTCCTTCA GAATCGGGCC TGAGCACTTC 
ACGTGCACGG GGAAAACCGT CATCTCTCCA GGCTTCACGG AGATCATGCC TTGGCAGAGT 
GTGCCTCTGG AGGAGAGCCT GCCCACCTGC CAGAGGGGCG ACTGCTTTCC TGTGAGCGAG 
ATGAAGATGC TAGAGAAGCA GACCAGCCCG CCCGACTACC TGACGGAGGC TGAGCTCATC 
ACACTCATGG AGAAGCACGG CATTGGGACA GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT CACTGTGGAG AGCGGGCGCC GCCTCAAACC CACCAACCTG 
GGCATCGTCC TGGTGCACGG CTACTACAAG ATTGATGCAG AGCTCGTGCT CCCCACTATC 
CGCAGCGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCTGA CTACCGCCAG 
GTCCTGGGCC ACACGCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTTGT GGACTCCATC 
GCCGCCACGG GCAAGCCCCT CTCCCGCTGT GGAAAGTGCC ACCGGTTCAT GAAGTACATT 
CAGGCCAAGC CAAGCCGCCT ACACTGCTCA CACTGTGACG AGACCTACAC CCTCCCCCAG 
AATGGCACCA TCAAGCTCTA CAAGGAGCTC CGGTGTCCAC TGGATGACTT CGAGCTGGTC 
CTGTGGTCAT CGGGCTCTCG GGGCAAGAGC TACCCACTGT GCCCCTATTG CTCCAACCAC 
CCACCCTTCC GAGACATGAA GAAAGGCATG GGCTGCAATG AGTGCACACA CCCCACCTGC 
CAGCACTCAC TGAGCATGCT GGGCATCGGC CAGTGCGTGG AATGTGAGAG CGGCGTGCTG 
GTGCTGGACC CCACCTCAGG CCCCAAGTGG AAGGTGGCCT GCAACAAGTG CAACGTGGTG 
GCACACTGCT TTGAGAATGC CTACCGTGTG CGGGTGTCTG CCGACACCTG CCCCACCTGT 
GAGGCTGCCC TGCTTGCCGT GGACTTCAAC AAGGCCAAAT CCCCGCTCCC AGGTGACGAG 
ACACAGCACA CAGGCTGCGT CTTCTGCGAC CCTGTCTTCC AGGAGCTGGT GGAGCTGAAG 
CACGCAGCCT CCTGCCACCC CATGCACCGG GGAGGGCCAG GGAGGAGGCA GGGCCGAGGG 
CGGGCTCGGG GCCGGCGGCC CACAGGGAAG CCCAGTGCCA GGCGGCCGAA GGACAAGATG 
TCAGCCCTGG CTGCCTACTT TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3B ( XM_005636634.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005636691.1 Canis lupus familiaris DNA topoisomerase III beta (TOP3B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe26760
Clone ID Related Accession (Same CDS sequence)	XM_005636634.2 , XM_005636634.3
Accession Version	XM_005636634.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DNA topoisomerase 3-beta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636634.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACCG TGCTCATGGT TGCAGAAAAG CCATCCTTGG CCCAGTCCAT TGCTAAAATC 
CTCTCTCGGG GAAACATGTC CTCCCACAAA GGGCTGAACG GAACATGCTC CGTGCATGAG 
TACAGCGGGA CCTTTGCTGG CCAGCCCGTC CGCTTCAAGA TGACATCCGT CTGTGGTCAT 
GTGATGACCT TGGATTTCCT GGGAAAGTAC AACAAATGGG ACAAGGTGGA CCCCGCAGAG 
CTGTTCAGCC AAGCCCCGAC AGAGAAGAAG GAGGCTAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAA AGGCTGCGAC TATATTGTGC TATGGCTGGA CTGCGACAAG 
GAGGGAGAGA ACATCTGTTT TGAGGTCCTG GACGCTGTGC TGCCCGTCAT GAATCCAACC 
CATAGTGGCG AAAAGACCGT GTTCCGGGCC AGGTTCAGCT CCATCACAGA CACGGACATC 
TGTGCTGCCA TGGCCCGCCT GGGCGAGCCA GACCACAACG AGGCGCTGTC GGTGGACGCC 
AGGCAAGAGC TGGACCTGCG CATCGGCTGT GCGTTCACCA GATTTCAAAC TAAGTACTTC 
CAGGGAAAAT ATGGCAATCT AGACAGCTCC CTCATCTCCT TTGGGCCATG TCAAACCCCT 
ACCCTGGGCT TCTGCGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC GGAGACGTAC 
TGGGTGCTGC AGGCCAAGGT CAATGCCGAT AAAGACAGAT CTCTTCTTTT GGACTGGGAC 
CGAGTACGAG TGTTCGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG 
AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAGAAGG CCAAGCAGAG GCCCCTCGCC 
CTTAACACTG TGGAGATGCT GCGTGTCGCT AGCTCTTCTC TGGGCATGGG GCCACAGCAT 
GCCATGCAGA CCGCTGAGCG CCTCTACACG CAAGGCTACA TCAGCTACCC GAGGACTGAG 
ACTACCCACT ACCCCGAGAA CTTTGACCTG AAGGGCCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CCGACACGGT AAAGCGCTTG TTAGCCGAAG GTATCAACCG GCCACGGAAA 
GGCCATGATG CCGGCGACCA TCCTCCCATC ACCCCCATGA GATCTGCCAC AGAGGCTGAA 
TTAGGGAGTG AGGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACTGTT 
AGCTATGACT GCAAGTACCT CCAGAGCACC ATTTCCTTCA GAATCGGGCC TGAGCACTTC 
ACGTGCACGG GGAAAACCGT CATCTCTCCA GGCTTCACGG AGATCATGCC TTGGCAGAGT 
GTGCCTCTGG AGGAGAGCCT GCCCACCTGC CAGAGGGGCG ACTGCTTTCC TGTGAGCGAG 
ATGAAGATGC TAGAGAAGCA GACCAGCCCG CCCGACTACC TGACGGAGGC TGAGCTCATC 
ACACTCATGG AGAAGCACGG CATTGGGACA GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT CACTGTGGAG AGCGGGCGCC GCCTCAAACC CACCAACCTG 
GGCATCGTCC TGGTGCACGG CTACTACAAG ATTGATGCAG AGCTCGTGCT CCCCACTATC 
CGCAGCGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCTGA CTACCGCCAG 
GTCCTGGGCC ACACGCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTTGT GGACTCCATC 
GCCGCCACGG GCAAGCCCCT CTCCCGCTGT GGAAAGTGCC ACCGGTTCAT GAAGTACATT 
CAGGCCAAGC CAAGCCGCCT ACACTGCTCA CACTGTGACG AGACCTACAC CCTCCCCCAG 
AATGGCACCA TCAAGCTCTA CAAGGAGCTC CGGTGTCCAC TGGATGACTT CGAGCTGGTC 
CTGTGGTCAT CGGGCTCTCG GGGCAAGAGC TACCCACTGT GCCCCTATTG CTCCAACCAC 
CCACCCTTCC GAGACATGAA GAAAGGCATG GGCTGCAATG AGTGCACACA CCCCACCTGC 
CAGCACTCAC TGAGCATGCT GGGCATCGGC CAGTGCGTGG AATGTGAGAG CGGCGTGCTG 
GTGCTGGACC CCACCTCAGG CCCCAAGTGG AAGGTGGCCT GCAACAAGTG CAACGTGGTG 
GCACACTGCT TTGAGAATGC CTACCGTGTG CGGGTGTCTG CCGACACCTG CCCCACCTGT 
GAGGCTGCCC TGCTTGCCGT GGACTTCAAC AAGGCCAAAT CCCCGCTCCC AGGTGACGAG 
ACACAGCACA CAGGCTGCGT CTTCTGCGAC CCTGTCTTCC AGGAGCTGGT GGAGCTGAAG 
CACGCAGCCT CCTGCCACCC CATGCACCGG GGAGGGCCAG GGAGGAGGCA GGGCCGAGGG 
CGGGCTCGGG GCCGGCGGCC CACAGGGAAG CCCAGTGCCA GGCGGCCGAA GGACAAGATG 
TCAGCCCTGG CTGCCTACTT TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005636691.1
CDS	755..3301
Translation	MKTVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGTCSVHEYSGTFAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGKGCDYIVLWLDCDKEGENICFEVLDAVLPVMNPTHSGEKTVFRARFSSITDTDICAAMARLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNADKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGPLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMRSATEAELGSEAWRLYEYITRHFIATVSYDCKYLQSTISFRIGPEHFTCTGKTVISPGFTEIMPWQSVPLEESLPTCQRGDCFPVSEMKMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCSNHPPFRDMKKGMGCNECTHPTCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAYRVRVSADTCPTCEAALLAVDFNKAKSPLPGDETQHTGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRARGRRPTGKPSARRPKDKMSALAAYFV

Target ORF information:

RefSeq Version	XM_005636634.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DNA topoisomerase III beta (TOP3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005636634.3

ORF Insert Sequence:

ATGAAGACCG TGCTCATGGT TGCAGAAAAG CCATCCTTGG CCCAGTCCAT TGCTAAAATC 
CTCTCTCGGG GAAACATGTC CTCCCACAAA GGGCTGAACG GAACATGCTC CGTGCATGAG 
TACAGCGGGA CCTTTGCTGG CCAGCCCGTC CGCTTCAAGA TGACATCCGT CTGTGGTCAT 
GTGATGACCT TGGATTTCCT GGGAAAGTAC AACAAATGGG ACAAGGTGGA CCCCGCAGAG 
CTGTTCAGCC AAGCCCCGAC AGAGAAGAAG GAGGCTAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAA AGGCTGCGAC TATATTGTGC TATGGCTGGA CTGCGACAAG 
GAGGGAGAGA ACATCTGTTT TGAGGTCCTG GACGCTGTGC TGCCCGTCAT GAATCCAACC 
CATAGTGGCG AAAAGACCGT GTTCCGGGCC AGGTTCAGCT CCATCACAGA CACGGACATC 
TGTGCTGCCA TGGCCCGCCT GGGCGAGCCA GACCACAACG AGGCGCTGTC GGTGGACGCC 
AGGCAAGAGC TGGACCTGCG CATCGGCTGT GCGTTCACCA GATTTCAAAC TAAGTACTTC 
CAGGGAAAAT ATGGCAATCT AGACAGCTCC CTCATCTCCT TTGGGCCATG TCAAACCCCT 
ACCCTGGGCT TCTGCGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC GGAGACGTAC 
TGGGTGCTGC AGGCCAAGGT CAATGCCGAT AAAGACAGAT CTCTTCTTTT GGACTGGGAC 
CGAGTACGAG TGTTCGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG 
AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAGAAGG CCAAGCAGAG GCCCCTCGCC 
CTTAACACTG TGGAGATGCT GCGTGTCGCT AGCTCTTCTC TGGGCATGGG GCCACAGCAT 
GCCATGCAGA CCGCTGAGCG CCTCTACACG CAAGGCTACA TCAGCTACCC GAGGACTGAG 
ACTACCCACT ACCCCGAGAA CTTTGACCTG AAGGGCCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CCGACACGGT AAAGCGCTTG TTAGCCGAAG GTATCAACCG GCCACGGAAA 
GGCCATGATG CCGGCGACCA TCCTCCCATC ACCCCCATGA GATCTGCCAC AGAGGCTGAA 
TTAGGGAGTG AGGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACTGTT 
AGCTATGACT GCAAGTACCT CCAGAGCACC ATTTCCTTCA GAATCGGGCC TGAGCACTTC 
ACGTGCACGG GGAAAACCGT CATCTCTCCA GGCTTCACGG AGATCATGCC TTGGCAGAGT 
GTGCCTCTGG AGGAGAGCCT GCCCACCTGC CAGAGGGGCG ACTGCTTTCC TGTGAGCGAG 
ATGAAGATGC TAGAGAAGCA GACCAGCCCG CCCGACTACC TGACGGAGGC TGAGCTCATC 
ACACTCATGG AGAAGCACGG CATTGGGACA GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT CACTGTGGAG AGCGGGCGCC GCCTCAAACC CACCAACCTG 
GGCATCGTCC TGGTGCACGG CTACTACAAG ATTGATGCAG AGCTCGTGCT CCCCACTATC 
CGCAGCGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCTGA CTACCGCCAG 
GTCCTGGGCC ACACGCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTTGT GGACTCCATC 
GCCGCCACGG GCAAGCCCCT CTCCCGCTGT GGAAAGTGCC ACCGGTTCAT GAAGTACATT 
CAGGCCAAGC CAAGCCGCCT ACACTGCTCA CACTGTGACG AGACCTACAC CCTCCCCCAG 
AATGGCACCA TCAAGCTCTA CAAGGAGCTC CGGTGTCCAC TGGATGACTT CGAGCTGGTC 
CTGTGGTCAT CGGGCTCTCG GGGCAAGAGC TACCCACTGT GCCCCTATTG CTCCAACCAC 
CCACCCTTCC GAGACATGAA GAAAGGCATG GGCTGCAATG AGTGCACACA CCCCACCTGC 
CAGCACTCAC TGAGCATGCT GGGCATCGGC CAGTGCGTGG AATGTGAGAG CGGCGTGCTG 
GTGCTGGACC CCACCTCAGG CCCCAAGTGG AAGGTGGCCT GCAACAAGTG CAACGTGGTG 
GCACACTGCT TTGAGAATGC CTACCGTGTG CGGGTGTCTG CCGACACCTG CCCCACCTGT 
GAGGCTGCCC TGCTTGCCGT GGACTTCAAC AAGGCCAAAT CCCCGCTCCC AGGTGACGAG 
ACACAGCACA CAGGCTGCGT CTTCTGCGAC CCTGTCTTCC AGGAGCTGGT GGAGCTGAAG 
CACGCAGCCT CCTGCCACCC CATGCACCGG GGAGGGCCAG GGAGGAGGCA GGGCCGAGGG 
CGGGCTCGGG GCCGGCGGCC CACAGGGAAG CCCAGTGCCA GGCGGCCGAA GGACAAGATG 
TCAGCCCTGG CTGCCTACTT TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3B ( XM_014108221.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963696.1 Canis lupus familiaris DNA topoisomerase III beta (TOP3B), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe43496
Clone ID Related Accession (Same CDS sequence)	XM_014108221.2 , XM_014108221.1
Accession Version	XM_014108221.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DNA topoisomerase 3-beta-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108221.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGT TCCTGCAGGT GGAGGGCAAA GGCTGCGACT ATATTGTGCT ATGGCTGGAC 
TGCGACAAGG AGGGAGAGAA CATCTGTTTT GAGGTCCTGG ACGCTGTGCT GCCCGTCATG 
AATCCAACCC ATAGTGGCGA AAAGACCGTG TTCCGGGCCA GGTTCAGCTC CATCACAGAC 
ACGGACATCT GTGCTGCCAT GGCCCGCCTG GGCGAGCCAG ACCACAACGA GGCGCTGTCG 
GTGGACGCCA GGCAAGAGCT GGACCTGCGC ATCGGCTGTG CGTTCACCAG ATTTCAAACT 
AAGTACTTCC AGGGAAAATA TGGCAATCTA GACAGCTCCC TCATCTCCTT TGGGCCATGT 
CAAACCCCTA CCCTGGGCTT CTGCGTGGAG AGACATGATA AAATCCAGTC CTTCAAACCG 
GAGACGTACT GGGTGCTGCA GGCCAAGGTC AATGCCGATA AAGACAGATC TCTTCTTTTG 
GACTGGGACC GAGTACGAGT GTTCGACCGG GAGATCGCAC AGATGTTTTT AAACATGACA 
AAGCTGGAGA AGGAAGCCCA GGTGGAGGCC ACAAGCAGGA AAGAGAAGGC CAAGCAGAGG 
CCCCTCGCCC TTAACACTGT GGAGATGCTG CGTGTCGCTA GCTCTTCTCT GGGCATGGGG 
CCACAGCATG CCATGCAGAC CGCTGAGCGC CTCTACACGC AAGGCTACAT CAGCTACCCG 
AGGACTGAGA CTACCCACTA CCCCGAGAAC TTTGACCTGA AGGGCCCTCT GCGGCAGCAG 
GCCAACCACC CCTACTGGGC CGACACGGTA AAGCGCTTGT TAGCCGAAGG TATCAACCGG 
CCACGGAAAG GCCATGATGC CGGCGACCAT CCTCCCATCA CCCCCATGAG ATCTGCCACA 
GAGGCTGAAT TAGGGAGTGA GGCGTGGCGG CTCTATGAGT ACATCACCAG ACACTTCATC 
GCCACTGTTA GCTATGACTG CAAGTACCTC CAGAGCACCA TTTCCTTCAG AATCGGGCCT 
GAGCACTTCA CGTGCACGGG GAAAACCGTC ATCTCTCCAG GCTTCACGGA GATCATGCCT 
TGGCAGAGTG TGCCTCTGGA GGAGAGCCTG CCCACCTGCC AGAGGGGCGA CTGCTTTCCT 
GTGAGCGAGA TGAAGATGCT AGAGAAGCAG ACCAGCCCGC CCGACTACCT GACGGAGGCT 
GAGCTCATCA CACTCATGGA GAAGCACGGC ATTGGGACAG ACGCCAGCAT CCCCGTGCAC 
ATCAACAACA TCTGCCAGCG CAACTACGTC ACTGTGGAGA GCGGGCGCCG CCTCAAACCC 
ACCAACCTGG GCATCGTCCT GGTGCACGGC TACTACAAGA TTGATGCAGA GCTCGTGCTC 
CCCACTATCC GCAGCGCAGT GGAGAAGCAG CTGAACCTGA TCGCCCAGGG CAAGGCTGAC 
TACCGCCAGG TCCTGGGCCA CACGCTGGAC GTCTTCAAGA GGAAGTTCCA CTACTTTGTG 
GACTCCATCG CCGGGATGGA CGAGCTGATG GAGGTGTCTT TTTCACCCCT GGCAGCCACG 
GGCAAGCCCC TCTCCCGCTG TGGAAAGTGC CACCGGTTCA TGAAGTACAT TCAGGCCAAG 
CCAAGCCGCC TACACTGCTC ACACTGTGAC GAGACCTACA CCCTCCCCCA GAATGGCACC 
ATCAAGCTCT ACAAGGAGCT CCGGTGTCCA CTGGATGACT TCGAGCTGGT CCTGTGGTCA 
TCGGGCTCTC GGGGCAAGAG CTACCCACTG TGCCCCTATT GCTCCAACCA CCCACCCTTC 
CGAGACATGA AGAAAGGCAT GGGCTGCAAT GAGTGCACAC ACCCCACCTG CCAGCACTCA 
CTGAGCATGC TGGGCATCGG CCAGTGCGTG GAATGTGAGA GCGGCGTGCT GGTGCTGGAC 
CCCACCTCAG GCCCCAAGTG GAAGGTGGCC TGCAACAAGT GCAACGTGGT GGCACACTGC 
TTTGAGAATG CCTACCGTGT GCGGGTGTCT GCCGACACCT GCCCCACCTG TGAGGCTGCC 
CTGCTTGCCG TGGACTTCAA CAAGGCCAAA TCCCCGCTCC CAGGTGACGA GACACAGCAC 
ACAGGCTGCG TCTTCTGCGA CCCTGTCTTC CAGGAGCTGG TGGAGCTGAA GCACGCAGCC 
TCCTGCCACC CCATGCACCG GGGAGGGCCA GGGAGGAGGC AGGGCCGAGG GCGGGCTCGG 
GGCCGGCGGC CCACAGGGAA GCCCAGTGCC AGGCGGCCGA AGGACAAGAT GTCAGCCCTG 
GCTGCCTACT TTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963696.1
CDS	369..2666
Translation	MVKFLQVEGKGCDYIVLWLDCDKEGENICFEVLDAVLPVMNPTHSGEKTVFRARFSSITDTDICAAMARLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNADKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGPLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMRSATEAELGSEAWRLYEYITRHFIATVSYDCKYLQSTISFRIGPEHFTCTGKTVISPGFTEIMPWQSVPLEESLPTCQRGDCFPVSEMKMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCSNHPPFRDMKKGMGCNECTHPTCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAYRVRVSADTCPTCEAALLAVDFNKAKSPLPGDETQHTGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRARGRRPTGKPSARRPKDKMSALAAYFV

Target ORF information:

RefSeq Version	XM_014108221.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DNA topoisomerase III beta (TOP3B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108221.2

ORF Insert Sequence:

ATGGTGAAGT TCCTGCAGGT GGAGGGCAAA GGCTGCGACT ATATTGTGCT ATGGCTGGAC 
TGCGACAAGG AGGGAGAGAA CATCTGTTTT GAGGTCCTGG ACGCTGTGCT GCCCGTCATG 
AATCCAACCC ATAGTGGCGA AAAGACCGTG TTCCGGGCCA GGTTCAGCTC CATCACAGAC 
ACGGACATCT GTGCTGCCAT GGCCCGCCTG GGCGAGCCAG ACCACAACGA GGCGCTGTCG 
GTGGACGCCA GGCAAGAGCT GGACCTGCGC ATCGGCTGTG CGTTCACCAG ATTTCAAACT 
AAGTACTTCC AGGGAAAATA TGGCAATCTA GACAGCTCCC TCATCTCCTT TGGGCCATGT 
CAAACCCCTA CCCTGGGCTT CTGCGTGGAG AGACATGATA AAATCCAGTC CTTCAAACCG 
GAGACGTACT GGGTGCTGCA GGCCAAGGTC AATGCCGATA AAGACAGATC TCTTCTTTTG 
GACTGGGACC GAGTACGAGT GTTCGACCGG GAGATCGCAC AGATGTTTTT AAACATGACA 
AAGCTGGAGA AGGAAGCCCA GGTGGAGGCC ACAAGCAGGA AAGAGAAGGC CAAGCAGAGG 
CCCCTCGCCC TTAACACTGT GGAGATGCTG CGTGTCGCTA GCTCTTCTCT GGGCATGGGG 
CCACAGCATG CCATGCAGAC CGCTGAGCGC CTCTACACGC AAGGCTACAT CAGCTACCCG 
AGGACTGAGA CTACCCACTA CCCCGAGAAC TTTGACCTGA AGGGCCCTCT GCGGCAGCAG 
GCCAACCACC CCTACTGGGC CGACACGGTA AAGCGCTTGT TAGCCGAAGG TATCAACCGG 
CCACGGAAAG GCCATGATGC CGGCGACCAT CCTCCCATCA CCCCCATGAG ATCTGCCACA 
GAGGCTGAAT TAGGGAGTGA GGCGTGGCGG CTCTATGAGT ACATCACCAG ACACTTCATC 
GCCACTGTTA GCTATGACTG CAAGTACCTC CAGAGCACCA TTTCCTTCAG AATCGGGCCT 
GAGCACTTCA CGTGCACGGG GAAAACCGTC ATCTCTCCAG GCTTCACGGA GATCATGCCT 
TGGCAGAGTG TGCCTCTGGA GGAGAGCCTG CCCACCTGCC AGAGGGGCGA CTGCTTTCCT 
GTGAGCGAGA TGAAGATGCT AGAGAAGCAG ACCAGCCCGC CCGACTACCT GACGGAGGCT 
GAGCTCATCA CACTCATGGA GAAGCACGGC ATTGGGACAG ACGCCAGCAT CCCCGTGCAC 
ATCAACAACA TCTGCCAGCG CAACTACGTC ACTGTGGAGA GCGGGCGCCG CCTCAAACCC 
ACCAACCTGG GCATCGTCCT GGTGCACGGC TACTACAAGA TTGATGCAGA GCTCGTGCTC 
CCCACTATCC GCAGCGCAGT GGAGAAGCAG CTGAACCTGA TCGCCCAGGG CAAGGCTGAC 
TACCGCCAGG TCCTGGGCCA CACGCTGGAC GTCTTCAAGA GGAAGTTCCA CTACTTTGTG 
GACTCCATCG CCGGGATGGA CGAGCTGATG GAGGTGTCTT TTTCACCCCT GGCAGCCACG 
GGCAAGCCCC TCTCCCGCTG TGGAAAGTGC CACCGGTTCA TGAAGTACAT TCAGGCCAAG 
CCAAGCCGCC TACACTGCTC ACACTGTGAC GAGACCTACA CCCTCCCCCA GAATGGCACC 
ATCAAGCTCT ACAAGGAGCT CCGGTGTCCA CTGGATGACT TCGAGCTGGT CCTGTGGTCA 
TCGGGCTCTC GGGGCAAGAG CTACCCACTG TGCCCCTATT GCTCCAACCA CCCACCCTTC 
CGAGACATGA AGAAAGGCAT GGGCTGCAAT GAGTGCACAC ACCCCACCTG CCAGCACTCA 
CTGAGCATGC TGGGCATCGG CCAGTGCGTG GAATGTGAGA GCGGCGTGCT GGTGCTGGAC 
CCCACCTCAG GCCCCAAGTG GAAGGTGGCC TGCAACAAGT GCAACGTGGT GGCACACTGC 
TTTGAGAATG CCTACCGTGT GCGGGTGTCT GCCGACACCT GCCCCACCTG TGAGGCTGCC 
CTGCTTGCCG TGGACTTCAA CAAGGCCAAA TCCCCGCTCC CAGGTGACGA GACACAGCAC 
ACAGGCTGCG TCTTCTGCGA CCCTGTCTTC CAGGAGCTGG TGGAGCTGAA GCACGCAGCC 
TCCTGCCACC CCATGCACCG GGGAGGGCCA GGGAGGAGGC AGGGCCGAGG GCGGGCTCGG 
GGCCGGCGGC CCACAGGGAA GCCCAGTGCC AGGCGGCCGA AGGACAAGAT GTCAGCCCTG 
GCTGCCTACT TTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3B ( XM_014108221.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963696.1 Canis lupus familiaris topoisomerase (DNA) III beta (TOP3B), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe43496
Clone ID Related Accession (Same CDS sequence)	XM_014108221.2 , XM_014108221.1
Accession Version	XM_014108221.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	DNA topoisomerase 3-beta-1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGT TCCTGCAGGT GGAGGGCAAA GGCTGCGACT ATATTGTGCT ATGGCTGGAC 
TGCGACAAGG AGGGAGAGAA CATCTGTTTT GAGGTCCTGG ACGCTGTGCT GCCCGTCATG 
AATCCAACCC ATAGTGGCGA AAAGACCGTG TTCCGGGCCA GGTTCAGCTC CATCACAGAC 
ACGGACATCT GTGCTGCCAT GGCCCGCCTG GGCGAGCCAG ACCACAACGA GGCGCTGTCG 
GTGGACGCCA GGCAAGAGCT GGACCTGCGC ATCGGCTGTG CGTTCACCAG ATTTCAAACT 
AAGTACTTCC AGGGAAAATA TGGCAATCTA GACAGCTCCC TCATCTCCTT TGGGCCATGT 
CAAACCCCTA CCCTGGGCTT CTGCGTGGAG AGACATGATA AAATCCAGTC CTTCAAACCG 
GAGACGTACT GGGTGCTGCA GGCCAAGGTC AATGCCGATA AAGACAGATC TCTTCTTTTG 
GACTGGGACC GAGTACGAGT GTTCGACCGG GAGATCGCAC AGATGTTTTT AAACATGACA 
AAGCTGGAGA AGGAAGCCCA GGTGGAGGCC ACAAGCAGGA AAGAGAAGGC CAAGCAGAGG 
CCCCTCGCCC TTAACACTGT GGAGATGCTG CGTGTCGCTA GCTCTTCTCT GGGCATGGGG 
CCACAGCATG CCATGCAGAC CGCTGAGCGC CTCTACACGC AAGGCTACAT CAGCTACCCG 
AGGACTGAGA CTACCCACTA CCCCGAGAAC TTTGACCTGA AGGGCCCTCT GCGGCAGCAG 
GCCAACCACC CCTACTGGGC CGACACGGTA AAGCGCTTGT TAGCCGAAGG TATCAACCGG 
CCACGGAAAG GCCATGATGC CGGCGACCAT CCTCCCATCA CCCCCATGAG ATCTGCCACA 
GAGGCTGAAT TAGGGAGTGA GGCGTGGCGG CTCTATGAGT ACATCACCAG ACACTTCATC 
GCCACTGTTA GCTATGACTG CAAGTACCTC CAGAGCACCA TTTCCTTCAG AATCGGGCCT 
GAGCACTTCA CGTGCACGGG GAAAACCGTC ATCTCTCCAG GCTTCACGGA GATCATGCCT 
TGGCAGAGTG TGCCTCTGGA GGAGAGCCTG CCCACCTGCC AGAGGGGCGA CTGCTTTCCT 
GTGAGCGAGA TGAAGATGCT AGAGAAGCAG ACCAGCCCGC CCGACTACCT GACGGAGGCT 
GAGCTCATCA CACTCATGGA GAAGCACGGC ATTGGGACAG ACGCCAGCAT CCCCGTGCAC 
ATCAACAACA TCTGCCAGCG CAACTACGTC ACTGTGGAGA GCGGGCGCCG CCTCAAACCC 
ACCAACCTGG GCATCGTCCT GGTGCACGGC TACTACAAGA TTGATGCAGA GCTCGTGCTC 
CCCACTATCC GCAGCGCAGT GGAGAAGCAG CTGAACCTGA TCGCCCAGGG CAAGGCTGAC 
TACCGCCAGG TCCTGGGCCA CACGCTGGAC GTCTTCAAGA GGAAGTTCCA CTACTTTGTG 
GACTCCATCG CCGGGATGGA CGAGCTGATG GAGGTGTCTT TTTCACCCCT GGCAGCCACG 
GGCAAGCCCC TCTCCCGCTG TGGAAAGTGC CACCGGTTCA TGAAGTACAT TCAGGCCAAG 
CCAAGCCGCC TACACTGCTC ACACTGTGAC GAGACCTACA CCCTCCCCCA GAATGGCACC 
ATCAAGCTCT ACAAGGAGCT CCGGTGTCCA CTGGATGACT TCGAGCTGGT CCTGTGGTCA 
TCGGGCTCTC GGGGCAAGAG CTACCCACTG TGCCCCTATT GCTCCAACCA CCCACCCTTC 
CGAGACATGA AGAAAGGCAT GGGCTGCAAT GAGTGCACAC ACCCCACCTG CCAGCACTCA 
CTGAGCATGC TGGGCATCGG CCAGTGCGTG GAATGTGAGA GCGGCGTGCT GGTGCTGGAC 
CCCACCTCAG GCCCCAAGTG GAAGGTGGCC TGCAACAAGT GCAACGTGGT GGCACACTGC 
TTTGAGAATG CCTACCGTGT GCGGGTGTCT GCCGACACCT GCCCCACCTG TGAGGCTGCC 
CTGCTTGCCG TGGACTTCAA CAAGGCCAAA TCCCCGCTCC CAGGTGACGA GACACAGCAC 
ACAGGCTGCG TCTTCTGCGA CCCTGTCTTC CAGGAGCTGG TGGAGCTGAA GCACGCAGCC 
TCCTGCCACC CCATGCACCG GGGAGGGCCA GGGAGGAGGC AGGGCCGAGG GCGGGCTCGG 
GGCCGGCGGC CCACAGGGAA GCCCAGTGCC AGGCGGCCGA AGGACAAGAT GTCAGCCCTG 
GCTGCCTACT TTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963696.1
CDS	322..2619
Translation	MVKFLQVEGKGCDYIVLWLDCDKEGENICFEVLDAVLPVMNPTHSGEKTVFRARFSSITDTDICAAMARLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNADKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGPLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMRSATEAELGSEAWRLYEYITRHFIATVSYDCKYLQSTISFRIGPEHFTCTGKTVISPGFTEIMPWQSVPLEESLPTCQRGDCFPVSEMKMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCSNHPPFRDMKKGMGCNECTHPTCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAYRVRVSADTCPTCEAALLAVDFNKAKSPLPGDETQHTGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRARGRRPTGKPSARRPKDKMSALAAYFV

Target ORF information:

RefSeq Version	XM_014108221.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris topoisomerase (DNA) III beta (TOP3B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108221.1

ORF Insert Sequence:

ATGGTGAAGT TCCTGCAGGT GGAGGGCAAA GGCTGCGACT ATATTGTGCT ATGGCTGGAC 
TGCGACAAGG AGGGAGAGAA CATCTGTTTT GAGGTCCTGG ACGCTGTGCT GCCCGTCATG 
AATCCAACCC ATAGTGGCGA AAAGACCGTG TTCCGGGCCA GGTTCAGCTC CATCACAGAC 
ACGGACATCT GTGCTGCCAT GGCCCGCCTG GGCGAGCCAG ACCACAACGA GGCGCTGTCG 
GTGGACGCCA GGCAAGAGCT GGACCTGCGC ATCGGCTGTG CGTTCACCAG ATTTCAAACT 
AAGTACTTCC AGGGAAAATA TGGCAATCTA GACAGCTCCC TCATCTCCTT TGGGCCATGT 
CAAACCCCTA CCCTGGGCTT CTGCGTGGAG AGACATGATA AAATCCAGTC CTTCAAACCG 
GAGACGTACT GGGTGCTGCA GGCCAAGGTC AATGCCGATA AAGACAGATC TCTTCTTTTG 
GACTGGGACC GAGTACGAGT GTTCGACCGG GAGATCGCAC AGATGTTTTT AAACATGACA 
AAGCTGGAGA AGGAAGCCCA GGTGGAGGCC ACAAGCAGGA AAGAGAAGGC CAAGCAGAGG 
CCCCTCGCCC TTAACACTGT GGAGATGCTG CGTGTCGCTA GCTCTTCTCT GGGCATGGGG 
CCACAGCATG CCATGCAGAC CGCTGAGCGC CTCTACACGC AAGGCTACAT CAGCTACCCG 
AGGACTGAGA CTACCCACTA CCCCGAGAAC TTTGACCTGA AGGGCCCTCT GCGGCAGCAG 
GCCAACCACC CCTACTGGGC CGACACGGTA AAGCGCTTGT TAGCCGAAGG TATCAACCGG 
CCACGGAAAG GCCATGATGC CGGCGACCAT CCTCCCATCA CCCCCATGAG ATCTGCCACA 
GAGGCTGAAT TAGGGAGTGA GGCGTGGCGG CTCTATGAGT ACATCACCAG ACACTTCATC 
GCCACTGTTA GCTATGACTG CAAGTACCTC CAGAGCACCA TTTCCTTCAG AATCGGGCCT 
GAGCACTTCA CGTGCACGGG GAAAACCGTC ATCTCTCCAG GCTTCACGGA GATCATGCCT 
TGGCAGAGTG TGCCTCTGGA GGAGAGCCTG CCCACCTGCC AGAGGGGCGA CTGCTTTCCT 
GTGAGCGAGA TGAAGATGCT AGAGAAGCAG ACCAGCCCGC CCGACTACCT GACGGAGGCT 
GAGCTCATCA CACTCATGGA GAAGCACGGC ATTGGGACAG ACGCCAGCAT CCCCGTGCAC 
ATCAACAACA TCTGCCAGCG CAACTACGTC ACTGTGGAGA GCGGGCGCCG CCTCAAACCC 
ACCAACCTGG GCATCGTCCT GGTGCACGGC TACTACAAGA TTGATGCAGA GCTCGTGCTC 
CCCACTATCC GCAGCGCAGT GGAGAAGCAG CTGAACCTGA TCGCCCAGGG CAAGGCTGAC 
TACCGCCAGG TCCTGGGCCA CACGCTGGAC GTCTTCAAGA GGAAGTTCCA CTACTTTGTG 
GACTCCATCG CCGGGATGGA CGAGCTGATG GAGGTGTCTT TTTCACCCCT GGCAGCCACG 
GGCAAGCCCC TCTCCCGCTG TGGAAAGTGC CACCGGTTCA TGAAGTACAT TCAGGCCAAG 
CCAAGCCGCC TACACTGCTC ACACTGTGAC GAGACCTACA CCCTCCCCCA GAATGGCACC 
ATCAAGCTCT ACAAGGAGCT CCGGTGTCCA CTGGATGACT TCGAGCTGGT CCTGTGGTCA 
TCGGGCTCTC GGGGCAAGAG CTACCCACTG TGCCCCTATT GCTCCAACCA CCCACCCTTC 
CGAGACATGA AGAAAGGCAT GGGCTGCAAT GAGTGCACAC ACCCCACCTG CCAGCACTCA 
CTGAGCATGC TGGGCATCGG CCAGTGCGTG GAATGTGAGA GCGGCGTGCT GGTGCTGGAC 
CCCACCTCAG GCCCCAAGTG GAAGGTGGCC TGCAACAAGT GCAACGTGGT GGCACACTGC 
TTTGAGAATG CCTACCGTGT GCGGGTGTCT GCCGACACCT GCCCCACCTG TGAGGCTGCC 
CTGCTTGCCG TGGACTTCAA CAAGGCCAAA TCCCCGCTCC CAGGTGACGA GACACAGCAC 
ACAGGCTGCG TCTTCTGCGA CCCTGTCTTC CAGGAGCTGG TGGAGCTGAA GCACGCAGCC 
TCCTGCCACC CCATGCACCG GGGAGGGCCA GGGAGGAGGC AGGGCCGAGG GCGGGCTCGG 
GGCCGGCGGC CCACAGGGAA GCCCAGTGCC AGGCGGCCGA AGGACAAGAT GTCAGCCCTG 
GCTGCCTACT TTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TOP3B cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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