TOP3B cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP3B
Entrez Gene ID	537418
Full Name	DNA topoisomerase III beta
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001206706.1	NP_001193635.1	DNA topoisomerase 3-beta-1
NM_001206706.2	NP_001193635.1	DNA topoisomerase 3-beta-1
XM_024977372.1	XP_024833140.1	DNA topoisomerase 3-beta-1 isoform X1
XM_024977374.1	XP_024833142.1	DNA topoisomerase 3-beta-1 isoform X2
XM_024977373.1	XP_024833141.1	DNA topoisomerase 3-beta-1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TOP3B	XP_515006.3
Rattus norvegicus (Norway rat)	Top3b	NP_001099331.1
Mus musculus (house mouse)	Top3b	NP_035754.1
Gallus gallus (chicken)	TOP3B	NP_001006181.1
Drosophila melanogaster (fruit fly)	Top3beta	NP_511059.2
Caenorhabditis elegans (roundworm)	Y48C3A.14	NP_001254381.1
Oryza sativa (rice)	Os09g0500600	NP_001063578.1
Homo sapiens (human)	TOP3B	NP_003926.1
Xenopus tropicalis (tropical clawed frog)	top3b	NP_001182501.1
	TOP3B	XP_001088462.2
Bos taurus (cattle)	TOP3B	NP_001193635.1
Danio rerio (zebrafish)	LOC793414	XP_003198651.1
Arabidopsis thaliana (thale cress)	AT2G32000	NP_180760.2
Canis lupus familiaris (dog)	TOP3B	XP_005636690.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TOP3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa224346	XM_024977373.1 Latest version!	Bos taurus DNA topoisomerase III beta (TOP3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa224346	XM_024977372.1 Latest version!	Bos taurus DNA topoisomerase III beta (TOP3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa224347	XM_024977374.1 Latest version!	Bos taurus DNA topoisomerase III beta (TOP3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa01739	NM_001206706.2 Latest version!	Bos taurus DNA topoisomerase III beta (TOP3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OBa01739	NM_001206706.1	Bos taurus topoisomerase (DNA) III beta (TOP3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP3B ( XM_024977372.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833140.1 Bos taurus DNA topoisomerase III beta (TOP3B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa224346
Clone ID Related Accession (Same CDS sequence)	XM_024977372.1 , XM_024977373.1
Accession Version	XM_024977372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA topoisomerase 3-beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACCG TGCTCATGGT GGCCGAGAAG CCGTCCTTGG CCCAGTCCAT CGCCAGGATC 
CTCTCTCGAG GCAGCATGTC CTCGCGCAAA GGGCTGAACG GGACCTGCTC GGTGCACGAG 
TACAGCGGGG CCTTCGAGGG GCAGCCGGCC CGCTTCAAGA TGACGTCCGT CTGCGGCCAC 
GTGATGACGC TGGACTTCCT GGGAAAGTAC AACAAGTGGG ATAAGGTGGA CCCCGCCGAG 
CTGTTTAGCC AGGCCCCGAC GGAGAAGAAG GAGGCCAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAG GGGCTGCGAC TGCATCGTGC TGTGGCTGGA CTGCGACAAG 
GAGGGCGAGA ACATCTGCTT CGAGGTGCTG GACGCGGTGC TGCCCGTCAT GAACCAGGCC 
CGCAGCGGCG AGCAGACAGT GTTCCGGGCG CGCTTCAGCT CCATCACGGA CACAGACATC 
TGCGCTGCCA TGGCCCGCCT GGGTGAGCCC GACCACAACG AGGCGCTCTC GGTGGACGCG 
CGGCAGGAGC TGGACCTCCG CATCGGCTGC GCCTTCACGA GGTTCCAGAC TAAATACTTC 
CAGGGGAAAT ACGGCAACCT GGACAGCTCG CTCATCTCCT TCGGGCCGTG CCAGACGCCC 
ACGCTGGGGT TCTGCGTGGA GAGGCATGAC AAGATCCAGT CCTTCAAGCC AGAGACCTAC 
TGGGTGCTAC AGGCCAAGGT GGATGTTGAT AAGGACCGCT CCCTCCTCCT GGACTGGGAC 
CGTGTGCGCG TGTTCGACCG GGAGATCGCA CAGATGTTCC TGAACATGAC CAAGCTCGAG 
AAGGAGGCCC AGGTGGAGGC CACAAGCAGG AAGGAGAAGG CCAAGCAGCG GCCGCTGGCC 
CTGAACACCG TGGAGATGCT GCGTGTGGCC AGCTCAGCCC TGGGAATGGG CCCGCAGCAC 
GCCATGCAGA CCGCCGAGCG GCTCTACACG CAGGGCTACA TCAGCTACCC GCGCACCGAG 
ACCACCCACT ACCCCGAGAG CTTCGACCTG AAGGGGCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CTGACACGGT GAAGCGGCTC TTAGCAGAAG GTATCAACCG CCCACGGAAA 
GGCCATGATG CTGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC GGAGGCCGAG 
TTAGGGGGCG AGGCTTGGCG GCTGTACGAA TACATCACCC GGCACTTCAT CGCCACCGTC 
AGCCACGACT GCAGGTACCT GCAGAGCAGC GTCTCCTTCC GGATCGGGCC CGAGCGCTTC 
ACCTGCACGG GCAAGGCCGT CATCTCCCCA GGCTTCACGG AGATCATGCC CTGGCAGAGC 
GTGCCCCTGG AGGAGAGCCT GCCCACCTGC CAGAAGGGCG ACACCCTGGC CGTGGCCGAG 
GTCAAGCTGC TGGAGAAGCA GACGAGCCCG CCGGACTACC TGACCGAGGC TGAGCTCATC 
ACCCTCATGG AGAAGCACGG CATCGGGACG GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT GATCGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTG 
GGCATCGTCC TGGTGCATGG CTACTACAAG ATCGATGCCG AGCTGGTGCT GCCCACCATC 
CGCAGCGCCG TGGAGAAGCA GCTGAACCTG ATCGCACAGG GCAGAGCCGA CTTCCGCCAA 
GTCCTGGGCC ACACCCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTCGT GGACTCCATC 
GCGGGCATGG ATGAACTGAT GGAGGTGTCC TTCTCGCCCC TGGCGGCCAC GGGCAAGCCC 
CTCTCCCGCT GCGGGAAGTG CCACCGGTTC ATGAAGTACA TCCAGGCCAA GCCGAGCCGC 
CTGCACTGCT CGCACTGCGA CGAGACCTAC ACGCTGCCCC AGAACGGCAC CATCAAGCTC 
TACAAGGAGC TGCGGTGCCC GCTGGACGAC TTCGAGCTGG TCCTGTGGTC GTCGGGCTCC 
AGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTCCAACC ACCCGCCCTT CCGGGACATG 
AAGAAGGGCA CGGGCTGCAA CGAGTGCACG CACCCCAGCT GCCAGCACTC GCTCAGCATG 
CTGGGCATCG GCCAGTGCGT GGAGTGCGAG AGCGGCGTGC TGGTGCTCGA CCCCACCTCC 
GGCCCCAAGT GGCGCGTGGC CTGCAACAGG TGCAACGTGG TGGCCCGCTG CTTCGAGAAC 
GCCCACCGCG TGCGCGTGTC CGCCGAGACG TGCGCCGCCT GCGAGGCAGC CCTGCTGGAC 
GTGGACTTCA ACAAGGCCAA GTCCCCGCTG CCGGGCGACG GCACGCAGCA CACGGGCTGC 
GTCTTCTGCG ACCCGGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GCGGGCCTGG GCGGAGGCAG GGCCGGGGCC GGGGCCGGGG CCGGAGGCCG 
GCGGGGAAGC CGGGTCCGCG CCGGCCCAAG GACAAGATGT CAGCTCTGGC CGCCTACTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833140.1
CDS	1060..3645
Translation	MKTVLMVAEKPSLAQSIARILSRGSMSSRKGLNGTCSVHEYSGAFEGQPARFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDCIVLWLDCDKEGENICFEVLDAVLPVMNQARSGEQTVFRARFSSITDTDICAAMARLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVDVDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSALGMGPQHAMQTAERLYTQGYISYPRTETTHYPESFDLKGPLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGEAWRLYEYITRHFIATVSHDCRYLQSSVSFRIGPERFTCTGKAVISPGFTEIMPWQSVPLEESLPTCQKGDTLAVAEVKLLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVIVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGRADFRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCSNHPPFRDMKKGTGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWRVACNRCNVVARCFENAHRVRVSAETCAACEAALLDVDFNKAKSPLPGDGTQHTGCVFCDPVFQELVELKHAASCHPMHRGPGRRQGRGRGRGRRPAGKPGPRRPKDKMSALAAYFV

Target ORF information:

RefSeq Version	XM_024977372.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA topoisomerase III beta (TOP3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977372.1

ORF Insert Sequence:

ATGAAGACCG TGCTCATGGT GGCCGAGAAG CCGTCCTTGG CCCAGTCCAT CGCCAGGATC 
CTCTCTCGAG GCAGCATGTC CTCGCGCAAA GGGCTGAACG GGACCTGCTC GGTGCACGAG 
TACAGCGGGG CCTTCGAGGG GCAGCCGGCC CGCTTCAAGA TGACGTCCGT CTGCGGCCAC 
GTGATGACGC TGGACTTCCT GGGAAAGTAC AACAAGTGGG ATAAGGTGGA CCCCGCCGAG 
CTGTTTAGCC AGGCCCCGAC GGAGAAGAAG GAGGCCAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAG GGGCTGCGAC TGCATCGTGC TGTGGCTGGA CTGCGACAAG 
GAGGGCGAGA ACATCTGCTT CGAGGTGCTG GACGCGGTGC TGCCCGTCAT GAACCAGGCC 
CGCAGCGGCG AGCAGACAGT GTTCCGGGCG CGCTTCAGCT CCATCACGGA CACAGACATC 
TGCGCTGCCA TGGCCCGCCT GGGTGAGCCC GACCACAACG AGGCGCTCTC GGTGGACGCG 
CGGCAGGAGC TGGACCTCCG CATCGGCTGC GCCTTCACGA GGTTCCAGAC TAAATACTTC 
CAGGGGAAAT ACGGCAACCT GGACAGCTCG CTCATCTCCT TCGGGCCGTG CCAGACGCCC 
ACGCTGGGGT TCTGCGTGGA GAGGCATGAC AAGATCCAGT CCTTCAAGCC AGAGACCTAC 
TGGGTGCTAC AGGCCAAGGT GGATGTTGAT AAGGACCGCT CCCTCCTCCT GGACTGGGAC 
CGTGTGCGCG TGTTCGACCG GGAGATCGCA CAGATGTTCC TGAACATGAC CAAGCTCGAG 
AAGGAGGCCC AGGTGGAGGC CACAAGCAGG AAGGAGAAGG CCAAGCAGCG GCCGCTGGCC 
CTGAACACCG TGGAGATGCT GCGTGTGGCC AGCTCAGCCC TGGGAATGGG CCCGCAGCAC 
GCCATGCAGA CCGCCGAGCG GCTCTACACG CAGGGCTACA TCAGCTACCC GCGCACCGAG 
ACCACCCACT ACCCCGAGAG CTTCGACCTG AAGGGGCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CTGACACGGT GAAGCGGCTC TTAGCAGAAG GTATCAACCG CCCACGGAAA 
GGCCATGATG CTGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC GGAGGCCGAG 
TTAGGGGGCG AGGCTTGGCG GCTGTACGAA TACATCACCC GGCACTTCAT CGCCACCGTC 
AGCCACGACT GCAGGTACCT GCAGAGCAGC GTCTCCTTCC GGATCGGGCC CGAGCGCTTC 
ACCTGCACGG GCAAGGCCGT CATCTCCCCA GGCTTCACGG AGATCATGCC CTGGCAGAGC 
GTGCCCCTGG AGGAGAGCCT GCCCACCTGC CAGAAGGGCG ACACCCTGGC CGTGGCCGAG 
GTCAAGCTGC TGGAGAAGCA GACGAGCCCG CCGGACTACC TGACCGAGGC TGAGCTCATC 
ACCCTCATGG AGAAGCACGG CATCGGGACG GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT GATCGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTG 
GGCATCGTCC TGGTGCATGG CTACTACAAG ATCGATGCCG AGCTGGTGCT GCCCACCATC 
CGCAGCGCCG TGGAGAAGCA GCTGAACCTG ATCGCACAGG GCAGAGCCGA CTTCCGCCAA 
GTCCTGGGCC ACACCCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTCGT GGACTCCATC 
GCGGGCATGG ATGAACTGAT GGAGGTGTCC TTCTCGCCCC TGGCGGCCAC GGGCAAGCCC 
CTCTCCCGCT GCGGGAAGTG CCACCGGTTC ATGAAGTACA TCCAGGCCAA GCCGAGCCGC 
CTGCACTGCT CGCACTGCGA CGAGACCTAC ACGCTGCCCC AGAACGGCAC CATCAAGCTC 
TACAAGGAGC TGCGGTGCCC GCTGGACGAC TTCGAGCTGG TCCTGTGGTC GTCGGGCTCC 
AGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTCCAACC ACCCGCCCTT CCGGGACATG 
AAGAAGGGCA CGGGCTGCAA CGAGTGCACG CACCCCAGCT GCCAGCACTC GCTCAGCATG 
CTGGGCATCG GCCAGTGCGT GGAGTGCGAG AGCGGCGTGC TGGTGCTCGA CCCCACCTCC 
GGCCCCAAGT GGCGCGTGGC CTGCAACAGG TGCAACGTGG TGGCCCGCTG CTTCGAGAAC 
GCCCACCGCG TGCGCGTGTC CGCCGAGACG TGCGCCGCCT GCGAGGCAGC CCTGCTGGAC 
GTGGACTTCA ACAAGGCCAA GTCCCCGCTG CCGGGCGACG GCACGCAGCA CACGGGCTGC 
GTCTTCTGCG ACCCGGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GCGGGCCTGG GCGGAGGCAG GGCCGGGGCC GGGGCCGGGG CCGGAGGCCG 
GCGGGGAAGC CGGGTCCGCG CCGGCCCAAG GACAAGATGT CAGCTCTGGC CGCCTACTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3B ( XM_024977373.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833141.1 Bos taurus DNA topoisomerase III beta (TOP3B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa224346
Clone ID Related Accession (Same CDS sequence)	XM_024977372.1 , XM_024977373.1
Accession Version	XM_024977373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA topoisomerase 3-beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACCG TGCTCATGGT GGCCGAGAAG CCGTCCTTGG CCCAGTCCAT CGCCAGGATC 
CTCTCTCGAG GCAGCATGTC CTCGCGCAAA GGGCTGAACG GGACCTGCTC GGTGCACGAG 
TACAGCGGGG CCTTCGAGGG GCAGCCGGCC CGCTTCAAGA TGACGTCCGT CTGCGGCCAC 
GTGATGACGC TGGACTTCCT GGGAAAGTAC AACAAGTGGG ATAAGGTGGA CCCCGCCGAG 
CTGTTTAGCC AGGCCCCGAC GGAGAAGAAG GAGGCCAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAG GGGCTGCGAC TGCATCGTGC TGTGGCTGGA CTGCGACAAG 
GAGGGCGAGA ACATCTGCTT CGAGGTGCTG GACGCGGTGC TGCCCGTCAT GAACCAGGCC 
CGCAGCGGCG AGCAGACAGT GTTCCGGGCG CGCTTCAGCT CCATCACGGA CACAGACATC 
TGCGCTGCCA TGGCCCGCCT GGGTGAGCCC GACCACAACG AGGCGCTCTC GGTGGACGCG 
CGGCAGGAGC TGGACCTCCG CATCGGCTGC GCCTTCACGA GGTTCCAGAC TAAATACTTC 
CAGGGGAAAT ACGGCAACCT GGACAGCTCG CTCATCTCCT TCGGGCCGTG CCAGACGCCC 
ACGCTGGGGT TCTGCGTGGA GAGGCATGAC AAGATCCAGT CCTTCAAGCC AGAGACCTAC 
TGGGTGCTAC AGGCCAAGGT GGATGTTGAT AAGGACCGCT CCCTCCTCCT GGACTGGGAC 
CGTGTGCGCG TGTTCGACCG GGAGATCGCA CAGATGTTCC TGAACATGAC CAAGCTCGAG 
AAGGAGGCCC AGGTGGAGGC CACAAGCAGG AAGGAGAAGG CCAAGCAGCG GCCGCTGGCC 
CTGAACACCG TGGAGATGCT GCGTGTGGCC AGCTCAGCCC TGGGAATGGG CCCGCAGCAC 
GCCATGCAGA CCGCCGAGCG GCTCTACACG CAGGGCTACA TCAGCTACCC GCGCACCGAG 
ACCACCCACT ACCCCGAGAG CTTCGACCTG AAGGGGCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CTGACACGGT GAAGCGGCTC TTAGCAGAAG GTATCAACCG CCCACGGAAA 
GGCCATGATG CTGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC GGAGGCCGAG 
TTAGGGGGCG AGGCTTGGCG GCTGTACGAA TACATCACCC GGCACTTCAT CGCCACCGTC 
AGCCACGACT GCAGGTACCT GCAGAGCAGC GTCTCCTTCC GGATCGGGCC CGAGCGCTTC 
ACCTGCACGG GCAAGGCCGT CATCTCCCCA GGCTTCACGG AGATCATGCC CTGGCAGAGC 
GTGCCCCTGG AGGAGAGCCT GCCCACCTGC CAGAAGGGCG ACACCCTGGC CGTGGCCGAG 
GTCAAGCTGC TGGAGAAGCA GACGAGCCCG CCGGACTACC TGACCGAGGC TGAGCTCATC 
ACCCTCATGG AGAAGCACGG CATCGGGACG GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT GATCGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTG 
GGCATCGTCC TGGTGCATGG CTACTACAAG ATCGATGCCG AGCTGGTGCT GCCCACCATC 
CGCAGCGCCG TGGAGAAGCA GCTGAACCTG ATCGCACAGG GCAGAGCCGA CTTCCGCCAA 
GTCCTGGGCC ACACCCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTCGT GGACTCCATC 
GCGGGCATGG ATGAACTGAT GGAGGTGTCC TTCTCGCCCC TGGCGGCCAC GGGCAAGCCC 
CTCTCCCGCT GCGGGAAGTG CCACCGGTTC ATGAAGTACA TCCAGGCCAA GCCGAGCCGC 
CTGCACTGCT CGCACTGCGA CGAGACCTAC ACGCTGCCCC AGAACGGCAC CATCAAGCTC 
TACAAGGAGC TGCGGTGCCC GCTGGACGAC TTCGAGCTGG TCCTGTGGTC GTCGGGCTCC 
AGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTCCAACC ACCCGCCCTT CCGGGACATG 
AAGAAGGGCA CGGGCTGCAA CGAGTGCACG CACCCCAGCT GCCAGCACTC GCTCAGCATG 
CTGGGCATCG GCCAGTGCGT GGAGTGCGAG AGCGGCGTGC TGGTGCTCGA CCCCACCTCC 
GGCCCCAAGT GGCGCGTGGC CTGCAACAGG TGCAACGTGG TGGCCCGCTG CTTCGAGAAC 
GCCCACCGCG TGCGCGTGTC CGCCGAGACG TGCGCCGCCT GCGAGGCAGC CCTGCTGGAC 
GTGGACTTCA ACAAGGCCAA GTCCCCGCTG CCGGGCGACG GCACGCAGCA CACGGGCTGC 
GTCTTCTGCG ACCCGGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GCGGGCCTGG GCGGAGGCAG GGCCGGGGCC GGGGCCGGGG CCGGAGGCCG 
GCGGGGAAGC CGGGTCCGCG CCGGCCCAAG GACAAGATGT CAGCTCTGGC CGCCTACTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833141.1
CDS	191..2776
Translation	MKTVLMVAEKPSLAQSIARILSRGSMSSRKGLNGTCSVHEYSGAFEGQPARFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDCIVLWLDCDKEGENICFEVLDAVLPVMNQARSGEQTVFRARFSSITDTDICAAMARLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVDVDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSALGMGPQHAMQTAERLYTQGYISYPRTETTHYPESFDLKGPLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGEAWRLYEYITRHFIATVSHDCRYLQSSVSFRIGPERFTCTGKAVISPGFTEIMPWQSVPLEESLPTCQKGDTLAVAEVKLLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVIVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGRADFRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCSNHPPFRDMKKGTGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWRVACNRCNVVARCFENAHRVRVSAETCAACEAALLDVDFNKAKSPLPGDGTQHTGCVFCDPVFQELVELKHAASCHPMHRGPGRRQGRGRGRGRRPAGKPGPRRPKDKMSALAAYFV

Target ORF information:

RefSeq Version	XM_024977373.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA topoisomerase III beta (TOP3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977373.1

ORF Insert Sequence:

ATGAAGACCG TGCTCATGGT GGCCGAGAAG CCGTCCTTGG CCCAGTCCAT CGCCAGGATC 
CTCTCTCGAG GCAGCATGTC CTCGCGCAAA GGGCTGAACG GGACCTGCTC GGTGCACGAG 
TACAGCGGGG CCTTCGAGGG GCAGCCGGCC CGCTTCAAGA TGACGTCCGT CTGCGGCCAC 
GTGATGACGC TGGACTTCCT GGGAAAGTAC AACAAGTGGG ATAAGGTGGA CCCCGCCGAG 
CTGTTTAGCC AGGCCCCGAC GGAGAAGAAG GAGGCCAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAG GGGCTGCGAC TGCATCGTGC TGTGGCTGGA CTGCGACAAG 
GAGGGCGAGA ACATCTGCTT CGAGGTGCTG GACGCGGTGC TGCCCGTCAT GAACCAGGCC 
CGCAGCGGCG AGCAGACAGT GTTCCGGGCG CGCTTCAGCT CCATCACGGA CACAGACATC 
TGCGCTGCCA TGGCCCGCCT GGGTGAGCCC GACCACAACG AGGCGCTCTC GGTGGACGCG 
CGGCAGGAGC TGGACCTCCG CATCGGCTGC GCCTTCACGA GGTTCCAGAC TAAATACTTC 
CAGGGGAAAT ACGGCAACCT GGACAGCTCG CTCATCTCCT TCGGGCCGTG CCAGACGCCC 
ACGCTGGGGT TCTGCGTGGA GAGGCATGAC AAGATCCAGT CCTTCAAGCC AGAGACCTAC 
TGGGTGCTAC AGGCCAAGGT GGATGTTGAT AAGGACCGCT CCCTCCTCCT GGACTGGGAC 
CGTGTGCGCG TGTTCGACCG GGAGATCGCA CAGATGTTCC TGAACATGAC CAAGCTCGAG 
AAGGAGGCCC AGGTGGAGGC CACAAGCAGG AAGGAGAAGG CCAAGCAGCG GCCGCTGGCC 
CTGAACACCG TGGAGATGCT GCGTGTGGCC AGCTCAGCCC TGGGAATGGG CCCGCAGCAC 
GCCATGCAGA CCGCCGAGCG GCTCTACACG CAGGGCTACA TCAGCTACCC GCGCACCGAG 
ACCACCCACT ACCCCGAGAG CTTCGACCTG AAGGGGCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CTGACACGGT GAAGCGGCTC TTAGCAGAAG GTATCAACCG CCCACGGAAA 
GGCCATGATG CTGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC GGAGGCCGAG 
TTAGGGGGCG AGGCTTGGCG GCTGTACGAA TACATCACCC GGCACTTCAT CGCCACCGTC 
AGCCACGACT GCAGGTACCT GCAGAGCAGC GTCTCCTTCC GGATCGGGCC CGAGCGCTTC 
ACCTGCACGG GCAAGGCCGT CATCTCCCCA GGCTTCACGG AGATCATGCC CTGGCAGAGC 
GTGCCCCTGG AGGAGAGCCT GCCCACCTGC CAGAAGGGCG ACACCCTGGC CGTGGCCGAG 
GTCAAGCTGC TGGAGAAGCA GACGAGCCCG CCGGACTACC TGACCGAGGC TGAGCTCATC 
ACCCTCATGG AGAAGCACGG CATCGGGACG GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT GATCGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTG 
GGCATCGTCC TGGTGCATGG CTACTACAAG ATCGATGCCG AGCTGGTGCT GCCCACCATC 
CGCAGCGCCG TGGAGAAGCA GCTGAACCTG ATCGCACAGG GCAGAGCCGA CTTCCGCCAA 
GTCCTGGGCC ACACCCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTCGT GGACTCCATC 
GCGGGCATGG ATGAACTGAT GGAGGTGTCC TTCTCGCCCC TGGCGGCCAC GGGCAAGCCC 
CTCTCCCGCT GCGGGAAGTG CCACCGGTTC ATGAAGTACA TCCAGGCCAA GCCGAGCCGC 
CTGCACTGCT CGCACTGCGA CGAGACCTAC ACGCTGCCCC AGAACGGCAC CATCAAGCTC 
TACAAGGAGC TGCGGTGCCC GCTGGACGAC TTCGAGCTGG TCCTGTGGTC GTCGGGCTCC 
AGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTCCAACC ACCCGCCCTT CCGGGACATG 
AAGAAGGGCA CGGGCTGCAA CGAGTGCACG CACCCCAGCT GCCAGCACTC GCTCAGCATG 
CTGGGCATCG GCCAGTGCGT GGAGTGCGAG AGCGGCGTGC TGGTGCTCGA CCCCACCTCC 
GGCCCCAAGT GGCGCGTGGC CTGCAACAGG TGCAACGTGG TGGCCCGCTG CTTCGAGAAC 
GCCCACCGCG TGCGCGTGTC CGCCGAGACG TGCGCCGCCT GCGAGGCAGC CCTGCTGGAC 
GTGGACTTCA ACAAGGCCAA GTCCCCGCTG CCGGGCGACG GCACGCAGCA CACGGGCTGC 
GTCTTCTGCG ACCCGGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GCGGGCCTGG GCGGAGGCAG GGCCGGGGCC GGGGCCGGGG CCGGAGGCCG 
GCGGGGAAGC CGGGTCCGCG CCGGCCCAAG GACAAGATGT CAGCTCTGGC CGCCTACTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3B ( XM_024977374.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833142.1 Bos taurus DNA topoisomerase III beta (TOP3B), transcript variant X3, mRNA.

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CloneID	OBa224347
Clone ID Related Accession (Same CDS sequence)	XM_024977374.1
Accession Version	XM_024977374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA topoisomerase 3-beta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACCG TGCTCATGGT GGCCGAGAAG CCGTCCTTGG CCCAGTCCAT CGCCAGGATC 
CTCTCTCGAG GCAGCATGTC CTCGCGCAAA GGGCTGAACG GGACCTGCTC GGTGCACGAG 
TACAGCGGGG CCTTCGAGGG GCAGCCGGCC CGCTTCAAGA TGACGTCCGT CTGCGGCCAC 
GTGATGACGC TGGACTTCCT GGGAAAGTAC AACAAGTGGG ATAAGGTGGA CCCCGCCGAG 
CTGTTTAGCC AGGCCCCGAC GGAGAAGAAG GAGGCCAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAG GGGCTGCGAC TGCATCGTGC TGTGGCTGGA CTGCGACAAG 
GAGGGCGAGA ACATCTGCTT CGAGGTGCTG GACGCGGTGC TGCCCGTCAT GAACCAGGCC 
CGCAGCGGCG AGCAGACAGT GTTCCGGGCG CGCTTCAGCT CCATCACGGA CACAGACATC 
TGCGCTGCCA TGGCCCGCCT GGGTGAGCCC GACCACAACG AGGCGCTCTC GGTGGACGCG 
CGGCAGGAGC TGGACCTCCG CATCGGCTGC GCCTTCACGA GGTTCCAGAC TAAATACTTC 
CAGGGGAAAT ACGGCAACCT GGACAGCTCG CTCATCTCCT TCGGGCCGTG CCAGACGCCC 
ACGCTGGGGT TCTGCGTGGA GAGGCATGAC AAGATCCAGT CCTTCAAGCC AGAGACCTAC 
TGGGTGCTAC AGGCCAAGGT GGATGTTGAT AAGGACCGCT CCCTCCTCCT GGACTGGGAC 
CGTGTGCGCG TGTTCGACCG GGAGATCGCA CAGATGTTCC TGAACATGAC CAAGCTCGAG 
AAGGAGGCCC AGGTGGAGGC CACAAGCAGG AAGGAGAAGG CCAAGCAGCG GCCGCTGGCC 
CTGAACACCG TGGAGATGCT GCGTGTGGCC AGCTCAGCCC TGGGAATGGG CCCGCAGCAC 
GCCATGCAGA CCGCCGAGCG GCTCTACACG CAGGGCTACA TCAGCTACCC GCGCACCGAG 
ACCACCCACT ACCCCGAGAG CTTCGACCTG AAGGGGCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CTGACACGGT GAAGCGGCTC TTAGCAGAAG GTATCAACCG CCCACGGAAA 
GGCCATGATG CTGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC GGAGGCCGAG 
TTAGGGGGCG AGGCTTGGCG GCTGTACGAA TACATCACCC GGCACTTCAT CGCCACCGTC 
AGCCACGACT GCAGGTACCT GCAGAGCAGC GTCTCCTTCC GGATCGGGCC CGAGCGCTTC 
ACCTGCACGG GCAAGGCCGT CATCTCCCCA GGCTTCACGG AGATCATGCC CTGGCAGAGC 
GTGCCCCTGG AGGAGAGCCT GCCCACCTGC CAGAAGGGCG ACACCCTGGC CGTGGCCGAG 
GTCAAGCTGC TGGAGAAGCA GACGAGCCCG CCGGACTACC TGACCGAGGC TGAGCTCATC 
ACCCTCATGG AGAAGCACGG CATCGATGCC GAGCTGGTGC TGCCCACCAT CCGCAGCGCC 
GTGGAGAAGC AGCTGAACCT GATCGCACAG GGCAGAGCCG ACTTCCGCCA AGTCCTGGGC 
CACACCCTGG ACGTCTTCAA GAGGAAGTTC CACTACTTCG TGGACTCCAT CGCGGGCATG 
GATGAACTGA TGGAGGTGTC CTTCTCGCCC CTGGCGGCCA CGGGCAAGCC CCTCTCCCGC 
TGCGGGAAGT GCCACCGGTT CATGAAGTAC ATCCAGGCCA AGCCGAGCCG CCTGCACTGC 
TCGCACTGCG ACGAGACCTA CACGCTGCCC CAGAACGGCA CCATCAAGCT CTACAAGGAG 
CTGCGGTGCC CGCTGGACGA CTTCGAGCTG GTCCTGTGGT CGTCGGGCTC CAGGGGCAAG 
AGCTACCCGC TGTGCCCCTA CTGCTCCAAC CACCCGCCCT TCCGGGACAT GAAGAAGGGC 
ACGGGCTGCA ACGAGTGCAC GCACCCCAGC TGCCAGCACT CGCTCAGCAT GCTGGGCATC 
GGCCAGTGCG TGGAGTGCGA GAGCGGCGTG CTGGTGCTCG ACCCCACCTC CGGCCCCAAG 
TGGCGCGTGG CCTGCAACAG GTGCAACGTG GTGGCCCGCT GCTTCGAGAA CGCCCACCGC 
GTGCGCGTGT CCGCCGAGAC GTGCGCCGCC TGCGAGGCAG CCCTGCTGGA CGTGGACTTC 
AACAAGGCCA AGTCCCCGCT GCCGGGCGAC GGCACGCAGC ACACGGGCTG CGTCTTCTGC 
GACCCGGTCT TCCAGGAGCT GGTGGAGCTG AAGCACGCAG CCTCCTGCCA CCCCATGCAC 
CGCGGGCCTG GGCGGAGGCA GGGCCGGGGC CGGGGCCGGG GCCGGAGGCC GGCGGGGAAG 
CCGGGTCCGC GCCGGCCCAA GGACAAGATG TCAGCTCTGG CCGCCTACTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833142.1
CDS	1061..3517
Translation	MKTVLMVAEKPSLAQSIARILSRGSMSSRKGLNGTCSVHEYSGAFEGQPARFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDCIVLWLDCDKEGENICFEVLDAVLPVMNQARSGEQTVFRARFSSITDTDICAAMARLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVDVDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSALGMGPQHAMQTAERLYTQGYISYPRTETTHYPESFDLKGPLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGEAWRLYEYITRHFIATVSHDCRYLQSSVSFRIGPERFTCTGKAVISPGFTEIMPWQSVPLEESLPTCQKGDTLAVAEVKLLEKQTSPPDYLTEAELITLMEKHGIDAELVLPTIRSAVEKQLNLIAQGRADFRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCSNHPPFRDMKKGTGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWRVACNRCNVVARCFENAHRVRVSAETCAACEAALLDVDFNKAKSPLPGDGTQHTGCVFCDPVFQELVELKHAASCHPMHRGPGRRQGRGRGRGRRPAGKPGPRRPKDKMSALAAYFV

Target ORF information:

RefSeq Version	XM_024977374.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA topoisomerase III beta (TOP3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977374.1

ORF Insert Sequence:

ATGAAGACCG TGCTCATGGT GGCCGAGAAG CCGTCCTTGG CCCAGTCCAT CGCCAGGATC 
CTCTCTCGAG GCAGCATGTC CTCGCGCAAA GGGCTGAACG GGACCTGCTC GGTGCACGAG 
TACAGCGGGG CCTTCGAGGG GCAGCCGGCC CGCTTCAAGA TGACGTCCGT CTGCGGCCAC 
GTGATGACGC TGGACTTCCT GGGAAAGTAC AACAAGTGGG ATAAGGTGGA CCCCGCCGAG 
CTGTTTAGCC AGGCCCCGAC GGAGAAGAAG GAGGCCAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAG GGGCTGCGAC TGCATCGTGC TGTGGCTGGA CTGCGACAAG 
GAGGGCGAGA ACATCTGCTT CGAGGTGCTG GACGCGGTGC TGCCCGTCAT GAACCAGGCC 
CGCAGCGGCG AGCAGACAGT GTTCCGGGCG CGCTTCAGCT CCATCACGGA CACAGACATC 
TGCGCTGCCA TGGCCCGCCT GGGTGAGCCC GACCACAACG AGGCGCTCTC GGTGGACGCG 
CGGCAGGAGC TGGACCTCCG CATCGGCTGC GCCTTCACGA GGTTCCAGAC TAAATACTTC 
CAGGGGAAAT ACGGCAACCT GGACAGCTCG CTCATCTCCT TCGGGCCGTG CCAGACGCCC 
ACGCTGGGGT TCTGCGTGGA GAGGCATGAC AAGATCCAGT CCTTCAAGCC AGAGACCTAC 
TGGGTGCTAC AGGCCAAGGT GGATGTTGAT AAGGACCGCT CCCTCCTCCT GGACTGGGAC 
CGTGTGCGCG TGTTCGACCG GGAGATCGCA CAGATGTTCC TGAACATGAC CAAGCTCGAG 
AAGGAGGCCC AGGTGGAGGC CACAAGCAGG AAGGAGAAGG CCAAGCAGCG GCCGCTGGCC 
CTGAACACCG TGGAGATGCT GCGTGTGGCC AGCTCAGCCC TGGGAATGGG CCCGCAGCAC 
GCCATGCAGA CCGCCGAGCG GCTCTACACG CAGGGCTACA TCAGCTACCC GCGCACCGAG 
ACCACCCACT ACCCCGAGAG CTTCGACCTG AAGGGGCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CTGACACGGT GAAGCGGCTC TTAGCAGAAG GTATCAACCG CCCACGGAAA 
GGCCATGATG CTGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC GGAGGCCGAG 
TTAGGGGGCG AGGCTTGGCG GCTGTACGAA TACATCACCC GGCACTTCAT CGCCACCGTC 
AGCCACGACT GCAGGTACCT GCAGAGCAGC GTCTCCTTCC GGATCGGGCC CGAGCGCTTC 
ACCTGCACGG GCAAGGCCGT CATCTCCCCA GGCTTCACGG AGATCATGCC CTGGCAGAGC 
GTGCCCCTGG AGGAGAGCCT GCCCACCTGC CAGAAGGGCG ACACCCTGGC CGTGGCCGAG 
GTCAAGCTGC TGGAGAAGCA GACGAGCCCG CCGGACTACC TGACCGAGGC TGAGCTCATC 
ACCCTCATGG AGAAGCACGG CATCGATGCC GAGCTGGTGC TGCCCACCAT CCGCAGCGCC 
GTGGAGAAGC AGCTGAACCT GATCGCACAG GGCAGAGCCG ACTTCCGCCA AGTCCTGGGC 
CACACCCTGG ACGTCTTCAA GAGGAAGTTC CACTACTTCG TGGACTCCAT CGCGGGCATG 
GATGAACTGA TGGAGGTGTC CTTCTCGCCC CTGGCGGCCA CGGGCAAGCC CCTCTCCCGC 
TGCGGGAAGT GCCACCGGTT CATGAAGTAC ATCCAGGCCA AGCCGAGCCG CCTGCACTGC 
TCGCACTGCG ACGAGACCTA CACGCTGCCC CAGAACGGCA CCATCAAGCT CTACAAGGAG 
CTGCGGTGCC CGCTGGACGA CTTCGAGCTG GTCCTGTGGT CGTCGGGCTC CAGGGGCAAG 
AGCTACCCGC TGTGCCCCTA CTGCTCCAAC CACCCGCCCT TCCGGGACAT GAAGAAGGGC 
ACGGGCTGCA ACGAGTGCAC GCACCCCAGC TGCCAGCACT CGCTCAGCAT GCTGGGCATC 
GGCCAGTGCG TGGAGTGCGA GAGCGGCGTG CTGGTGCTCG ACCCCACCTC CGGCCCCAAG 
TGGCGCGTGG CCTGCAACAG GTGCAACGTG GTGGCCCGCT GCTTCGAGAA CGCCCACCGC 
GTGCGCGTGT CCGCCGAGAC GTGCGCCGCC TGCGAGGCAG CCCTGCTGGA CGTGGACTTC 
AACAAGGCCA AGTCCCCGCT GCCGGGCGAC GGCACGCAGC ACACGGGCTG CGTCTTCTGC 
GACCCGGTCT TCCAGGAGCT GGTGGAGCTG AAGCACGCAG CCTCCTGCCA CCCCATGCAC 
CGCGGGCCTG GGCGGAGGCA GGGCCGGGGC CGGGGCCGGG GCCGGAGGCC GGCGGGGAAG 
CCGGGTCCGC GCCGGCCCAA GGACAAGATG TCAGCTCTGG CCGCCTACTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3B ( NM_001206706.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193635.1 Bos taurus topoisomerase (DNA) III beta (TOP3B), mRNA.

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CloneID	OBa01739
Clone ID Related Accession (Same CDS sequence)	NM_001206706.1 , NM_001206706.2
Accession Version	NM_001206706.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-10-25
Organism	Bos taurus(cattle)
Product	DNA topoisomerase 3-beta-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03127735.1, BT026532.1, EV701465.1, EE359622.1, CX735376.1 and AAFC03129757.1. On or before May 4, 2011 this sequence version replaced XM_617580.4, XM_002694700.1. Sequence Note: This RefSeq record was created from transcripts of different strains and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. COMPLETENESS: complete on the 3' end.

ATGAAGACCG TGCTCATGGT GGCCGAGAAG CCGTCCTTGG CCCAGTCCAT CGCCAGGATC 
CTCTCTCGAG GCAGCATGTC CTCGCGCAAA GGGCTGAACG GGACCTGCTC GGTGCACGAG 
TACAGCGGGG CCTTCGAGGG GCAGCCGGCC CGCTTCAAGA TGACGTCCGT CTGCGGCCAC 
GTGATGACGC TGGACTTCCT GGGAAAGTAC AACAAGTGGG ATAAGGTGGA CCCCGCCGAG 
CTGTTTAGCC AGGCCCCGAC GGAGAAGAAG GAGGCCAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAG GGGCTGCGAC TGCATCGTGC TGTGGCTGGA CTGCGACAAG 
GAGGGCGAGA ACATCTGCTT CGAGGTGCTG GACGCGGTGC TGCCCGTCAT GAACCAGGCC 
CGCAGCGGCG AGCAGACAGT GTTCCGGGCG CGCTTCAGCT CCATCACGGA CACAGACATC 
TGCGCTGCCA TGGCCCGCCT GGGTGAGCCC GACCACAACG AGGCGCTCTC GGTGGACGCG 
CGGCAGGAGC TGGACCTCCG CATCGGCTGC GCCTTCACGA GGTTCCAGAC TAAATACTTC 
CAGGGGAAAT ACGGCAACCT GGACAGCTCG CTCATCTCCT TCGGGCCGTG CCAGACGCCC 
ACGCTGGGGT TCTGCGTGGA GAGGCATGAC AAGATCCAGT CCTTCAAGCC AGAGACCTAC 
TGGGTGCTAC AGGCCAAGGT GGATGTTGAT AAGGACCGCT CCCTCCTCCT GGACTGGGAC 
CGTGTGCGCG TGTTCGACCG GGAGATCGCA CAGATGTTCC TGAACATGAC CAAGCTCGAG 
AAGGAGGCCC AGGTGGAGGC CACAAGCAGG AAGGAGAAGG CCAAGCAGCG GCCGCTGGCC 
CTGAACACCG TGGAGATGCT GCGTGTGGCC AGCTCAGCCC TGGGAATGGG CCCGCAGCAC 
GCCATGCAGA CCGCCGAGCG GCTCTACACG CAGGGCTACA TCAGCTACCC GCGCACCGAG 
ACCACCCACT ACCCCGAGAG CTTCGACCTG AAGGGGCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CTGACACGGT GAAGCGGCTC TTAGCAGAAG GTATCAACCG CCCACGGAAA 
GGCCATGATG CTGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC GGAGGCCGAG 
TTAGGGGGCG AGGCTTGGCG GCTGTACGAA TACATCACCC GGCACTTCAT CGCCACCGTC 
AGCCACGACT GCAGGTACCT GCAGAGCAGC GTCTCCTTCC GGATCGGGCC CGAGCGCTTC 
ACCTGCACGG GCAAGGCCGT CATCTCCCCA GGCTTCACGG AGATCATGCC CTGGCAGAGC 
GTGCCCCTGG AGGAGAGCCT GCCCACCTGC CAGAAGGGCG ACACCCTGGC CGTGGCTGAG 
GTGAAGCTGC TGGAGAAGCA GACGAGCCCG CCAGACTACC TGACCGAGGC TGAGCTCATC 
ACCCTCATGG AGAAGCACGG CATCGGGACG GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT GATCGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTG 
GGCATCGTCC TGGTGCATGG CTACTACAAG ATCGATGCCG AGCTGGTGCT GCCCACCATC 
CGCAGCGCCG TGGAGAAGCA GCTGAACCTG ATCGCACAGG GCAGAGCCGA CTTCCGCCAA 
GTCCTGGGCC ACACCCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTCGT GGACTCCATC 
GCGGGCATGG ATGAACTGAT GGAGGTGTCC TTCTCGCCCC TGGCGGCCAC GGGCAAGCCC 
CTCTCCCGCT GCGGGAAGTG CCACCGGTTC ATGAAGTACA TCCAGGCCAA GCCGAGCCGC 
CTGCACTGCT CGCACTGCGA CGAGACCTAC ACGCTGCCCC AGAACGGCAC CATCAAGCTC 
TACAAGGAGC TGCGGTGCCC GCTGGACGAC TTCGAGCTGG TCCTGTGGTC GTCGGGCTCC 
AGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTCCAACC ACCCGCCCTT CCGGGACATG 
AAGAAGGGCA CAGGCTGCAA CGAGTGCACG CACCCCAGCT GCCAGCACTC GCTCAGCATG 
CTGGGCATCG GCCAGTGCGT GGAGTGCGAG AGCGGCGTGC TGGTGCTCGA CCCCACCTCC 
GGCCCCAAGT GGCGCGTGGC CTGCAACAGG TGCAACGTGG TGGCCCGCTG CTTCGAGAAC 
GCCCACCGCG TGCGCGTGTC CGCCGAGACG TGCGCCGCCT GCGAGGCAGC CCTGCTGGAC 
GTGGACTTCA ACAAGGCCAA GTCCCCGCTG CCGGGCGACG GCACGCAGCA CACGGGCTGC 
GTCTTCTGCG ACCCGGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GCGGGCCTGG GCGGAGGCAG GGCCGGGGCC GGGGCCGGGG CCGGAGGCCG 
GCGGGGAAGC CGGGTCCGCG CCGGCCCAAG GACAAGATGT CAGCTCTGGC CGCCTACTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193635.1
CDS	175..2760
Translation	MKTVLMVAEKPSLAQSIARILSRGSMSSRKGLNGTCSVHEYSGAFEGQPARFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDCIVLWLDCDKEGENICFEVLDAVLPVMNQARSGEQTVFRARFSSITDTDICAAMARLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVDVDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSALGMGPQHAMQTAERLYTQGYISYPRTETTHYPESFDLKGPLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGEAWRLYEYITRHFIATVSHDCRYLQSSVSFRIGPERFTCTGKAVISPGFTEIMPWQSVPLEESLPTCQKGDTLAVAEVKLLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVIVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGRADFRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCSNHPPFRDMKKGTGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWRVACNRCNVVARCFENAHRVRVSAETCAACEAALLDVDFNKAKSPLPGDGTQHTGCVFCDPVFQELVELKHAASCHPMHRGPGRRQGRGRGRGRRPAGKPGPRRPKDKMSALAAYFV

Target ORF information:

RefSeq Version	NM_001206706.1
Organism	Bos taurus(cattle)
Definition	Bos taurus topoisomerase (DNA) III beta (TOP3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206706.1

ORF Insert Sequence:

ATGAAGACCG TGCTCATGGT GGCCGAGAAG CCGTCCTTGG CCCAGTCCAT CGCCAGGATC 
CTCTCTCGAG GCAGCATGTC CTCGCGCAAA GGGCTGAACG GGACCTGCTC GGTGCACGAG 
TACAGCGGGG CCTTCGAGGG GCAGCCGGCC CGCTTCAAGA TGACGTCCGT CTGCGGCCAC 
GTGATGACGC TGGACTTCCT GGGAAAGTAC AACAAGTGGG ATAAGGTGGA CCCCGCCGAG 
CTGTTTAGCC AGGCCCCGAC GGAGAAGAAG GAGGCCAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAG GGGCTGCGAC TGCATCGTGC TGTGGCTGGA CTGCGACAAG 
GAGGGCGAGA ACATCTGCTT CGAGGTGCTG GACGCGGTGC TGCCCGTCAT GAACCAGGCC 
CGCAGCGGCG AGCAGACAGT GTTCCGGGCG CGCTTCAGCT CCATCACGGA CACAGACATC 
TGCGCTGCCA TGGCCCGCCT GGGTGAGCCC GACCACAACG AGGCGCTCTC GGTGGACGCG 
CGGCAGGAGC TGGACCTCCG CATCGGCTGC GCCTTCACGA GGTTCCAGAC TAAATACTTC 
CAGGGGAAAT ACGGCAACCT GGACAGCTCG CTCATCTCCT TCGGGCCGTG CCAGACGCCC 
ACGCTGGGGT TCTGCGTGGA GAGGCATGAC AAGATCCAGT CCTTCAAGCC AGAGACCTAC 
TGGGTGCTAC AGGCCAAGGT GGATGTTGAT AAGGACCGCT CCCTCCTCCT GGACTGGGAC 
CGTGTGCGCG TGTTCGACCG GGAGATCGCA CAGATGTTCC TGAACATGAC CAAGCTCGAG 
AAGGAGGCCC AGGTGGAGGC CACAAGCAGG AAGGAGAAGG CCAAGCAGCG GCCGCTGGCC 
CTGAACACCG TGGAGATGCT GCGTGTGGCC AGCTCAGCCC TGGGAATGGG CCCGCAGCAC 
GCCATGCAGA CCGCCGAGCG GCTCTACACG CAGGGCTACA TCAGCTACCC GCGCACCGAG 
ACCACCCACT ACCCCGAGAG CTTCGACCTG AAGGGGCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CTGACACGGT GAAGCGGCTC TTAGCAGAAG GTATCAACCG CCCACGGAAA 
GGCCATGATG CTGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC GGAGGCCGAG 
TTAGGGGGCG AGGCTTGGCG GCTGTACGAA TACATCACCC GGCACTTCAT CGCCACCGTC 
AGCCACGACT GCAGGTACCT GCAGAGCAGC GTCTCCTTCC GGATCGGGCC CGAGCGCTTC 
ACCTGCACGG GCAAGGCCGT CATCTCCCCA GGCTTCACGG AGATCATGCC CTGGCAGAGC 
GTGCCCCTGG AGGAGAGCCT GCCCACCTGC CAGAAGGGCG ACACCCTGGC CGTGGCTGAG 
GTGAAGCTGC TGGAGAAGCA GACGAGCCCG CCAGACTACC TGACCGAGGC TGAGCTCATC 
ACCCTCATGG AGAAGCACGG CATCGGGACG GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT GATCGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTG 
GGCATCGTCC TGGTGCATGG CTACTACAAG ATCGATGCCG AGCTGGTGCT GCCCACCATC 
CGCAGCGCCG TGGAGAAGCA GCTGAACCTG ATCGCACAGG GCAGAGCCGA CTTCCGCCAA 
GTCCTGGGCC ACACCCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTCGT GGACTCCATC 
GCGGGCATGG ATGAACTGAT GGAGGTGTCC TTCTCGCCCC TGGCGGCCAC GGGCAAGCCC 
CTCTCCCGCT GCGGGAAGTG CCACCGGTTC ATGAAGTACA TCCAGGCCAA GCCGAGCCGC 
CTGCACTGCT CGCACTGCGA CGAGACCTAC ACGCTGCCCC AGAACGGCAC CATCAAGCTC 
TACAAGGAGC TGCGGTGCCC GCTGGACGAC TTCGAGCTGG TCCTGTGGTC GTCGGGCTCC 
AGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTCCAACC ACCCGCCCTT CCGGGACATG 
AAGAAGGGCA CAGGCTGCAA CGAGTGCACG CACCCCAGCT GCCAGCACTC GCTCAGCATG 
CTGGGCATCG GCCAGTGCGT GGAGTGCGAG AGCGGCGTGC TGGTGCTCGA CCCCACCTCC 
GGCCCCAAGT GGCGCGTGGC CTGCAACAGG TGCAACGTGG TGGCCCGCTG CTTCGAGAAC 
GCCCACCGCG TGCGCGTGTC CGCCGAGACG TGCGCCGCCT GCGAGGCAGC CCTGCTGGAC 
GTGGACTTCA ACAAGGCCAA GTCCCCGCTG CCGGGCGACG GCACGCAGCA CACGGGCTGC 
GTCTTCTGCG ACCCGGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GCGGGCCTGG GCGGAGGCAG GGCCGGGGCC GGGGCCGGGG CCGGAGGCCG 
GCGGGGAAGC CGGGTCCGCG CCGGCCCAAG GACAAGATGT CAGCTCTGGC CGCCTACTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3B ( NM_001206706.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193635.1 Bos taurus DNA topoisomerase III beta (TOP3B), mRNA.

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CloneID	OBa01739
Clone ID Related Accession (Same CDS sequence)	NM_001206706.1 , NM_001206706.2
Accession Version	NM_001206706.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	DNA topoisomerase 3-beta-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000017.1, BT026532.1, EV701465.1, EE359622.1 and CX735376.1. On May 30, 2018 this sequence version replaced NM_001206706.1. Sequence Note: This RefSeq record was created from transcripts of different strains and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## CDS exon combination :: SRR6764281.4972.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGACCG TGCTCATGGT GGCCGAGAAG CCGTCCTTGG CCCAGTCCAT CGCCAGGATC 
CTCTCTCGAG GCAGCATGTC CTCGCGCAAA GGGCTGAACG GGACCTGCTC GGTGCACGAG 
TACAGCGGGG CCTTCGAGGG GCAGCCGGCC CGCTTCAAGA TGACGTCCGT CTGCGGCCAC 
GTGATGACGC TGGACTTCCT GGGAAAGTAC AACAAGTGGG ATAAGGTGGA CCCCGCCGAG 
CTGTTTAGCC AGGCCCCGAC GGAGAAGAAG GAGGCCAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAG GGGCTGCGAC TGCATCGTGC TGTGGCTGGA CTGCGACAAG 
GAGGGCGAGA ACATCTGCTT CGAGGTGCTG GACGCGGTGC TGCCCGTCAT GAACCAGGCC 
CGCAGCGGCG AGCAGACAGT GTTCCGGGCG CGCTTCAGCT CCATCACGGA CACAGACATC 
TGCGCTGCCA TGGCCCGCCT GGGTGAGCCC GACCACAACG AGGCGCTCTC GGTGGACGCG 
CGGCAGGAGC TGGACCTCCG CATCGGCTGC GCCTTCACGA GGTTCCAGAC TAAATACTTC 
CAGGGGAAAT ACGGCAACCT GGACAGCTCG CTCATCTCCT TCGGGCCGTG CCAGACGCCC 
ACGCTGGGGT TCTGCGTGGA GAGGCATGAC AAGATCCAGT CCTTCAAGCC AGAGACCTAC 
TGGGTGCTAC AGGCCAAGGT GGATGTTGAT AAGGACCGCT CCCTCCTCCT GGACTGGGAC 
CGTGTGCGCG TGTTCGACCG GGAGATCGCA CAGATGTTCC TGAACATGAC CAAGCTCGAG 
AAGGAGGCCC AGGTGGAGGC CACAAGCAGG AAGGAGAAGG CCAAGCAGCG GCCGCTGGCC 
CTGAACACCG TGGAGATGCT GCGTGTGGCC AGCTCAGCCC TGGGAATGGG CCCGCAGCAC 
GCCATGCAGA CCGCCGAGCG GCTCTACACG CAGGGCTACA TCAGCTACCC GCGCACCGAG 
ACCACCCACT ACCCCGAGAG CTTCGACCTG AAGGGGCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CTGACACGGT GAAGCGGCTC TTAGCAGAAG GTATCAACCG CCCACGGAAA 
GGCCATGATG CTGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC GGAGGCCGAG 
TTAGGGGGCG AGGCTTGGCG GCTGTACGAA TACATCACCC GGCACTTCAT CGCCACCGTC 
AGCCACGACT GCAGGTACCT GCAGAGCAGC GTCTCCTTCC GGATCGGGCC CGAGCGCTTC 
ACCTGCACGG GCAAGGCCGT CATCTCCCCA GGCTTCACGG AGATCATGCC CTGGCAGAGC 
GTGCCCCTGG AGGAGAGCCT GCCCACCTGC CAGAAGGGCG ACACCCTGGC CGTGGCTGAG 
GTGAAGCTGC TGGAGAAGCA GACGAGCCCG CCAGACTACC TGACCGAGGC TGAGCTCATC 
ACCCTCATGG AGAAGCACGG CATCGGGACG GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT GATCGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTG 
GGCATCGTCC TGGTGCATGG CTACTACAAG ATCGATGCCG AGCTGGTGCT GCCCACCATC 
CGCAGCGCCG TGGAGAAGCA GCTGAACCTG ATCGCACAGG GCAGAGCCGA CTTCCGCCAA 
GTCCTGGGCC ACACCCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTCGT GGACTCCATC 
GCGGGCATGG ATGAACTGAT GGAGGTGTCC TTCTCGCCCC TGGCGGCCAC GGGCAAGCCC 
CTCTCCCGCT GCGGGAAGTG CCACCGGTTC ATGAAGTACA TCCAGGCCAA GCCGAGCCGC 
CTGCACTGCT CGCACTGCGA CGAGACCTAC ACGCTGCCCC AGAACGGCAC CATCAAGCTC 
TACAAGGAGC TGCGGTGCCC GCTGGACGAC TTCGAGCTGG TCCTGTGGTC GTCGGGCTCC 
AGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTCCAACC ACCCGCCCTT CCGGGACATG 
AAGAAGGGCA CAGGCTGCAA CGAGTGCACG CACCCCAGCT GCCAGCACTC GCTCAGCATG 
CTGGGCATCG GCCAGTGCGT GGAGTGCGAG AGCGGCGTGC TGGTGCTCGA CCCCACCTCC 
GGCCCCAAGT GGCGCGTGGC CTGCAACAGG TGCAACGTGG TGGCCCGCTG CTTCGAGAAC 
GCCCACCGCG TGCGCGTGTC CGCCGAGACG TGCGCCGCCT GCGAGGCAGC CCTGCTGGAC 
GTGGACTTCA ACAAGGCCAA GTCCCCGCTG CCGGGCGACG GCACGCAGCA CACGGGCTGC 
GTCTTCTGCG ACCCGGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GCGGGCCTGG GCGGAGGCAG GGCCGGGGCC GGGGCCGGGG CCGGAGGCCG 
GCGGGGAAGC CGGGTCCGCG CCGGCCCAAG GACAAGATGT CAGCTCTGGC CGCCTACTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193635.1
CDS	175..2760
Translation	MKTVLMVAEKPSLAQSIARILSRGSMSSRKGLNGTCSVHEYSGAFEGQPARFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDCIVLWLDCDKEGENICFEVLDAVLPVMNQARSGEQTVFRARFSSITDTDICAAMARLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVDVDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSALGMGPQHAMQTAERLYTQGYISYPRTETTHYPESFDLKGPLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGEAWRLYEYITRHFIATVSHDCRYLQSSVSFRIGPERFTCTGKAVISPGFTEIMPWQSVPLEESLPTCQKGDTLAVAEVKLLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVIVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGRADFRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCSNHPPFRDMKKGTGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWRVACNRCNVVARCFENAHRVRVSAETCAACEAALLDVDFNKAKSPLPGDGTQHTGCVFCDPVFQELVELKHAASCHPMHRGPGRRQGRGRGRGRRPAGKPGPRRPKDKMSALAAYFV

Target ORF information:

RefSeq Version	NM_001206706.2
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA topoisomerase III beta (TOP3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206706.2

ORF Insert Sequence:

ATGAAGACCG TGCTCATGGT GGCCGAGAAG CCGTCCTTGG CCCAGTCCAT CGCCAGGATC 
CTCTCTCGAG GCAGCATGTC CTCGCGCAAA GGGCTGAACG GGACCTGCTC GGTGCACGAG 
TACAGCGGGG CCTTCGAGGG GCAGCCGGCC CGCTTCAAGA TGACGTCCGT CTGCGGCCAC 
GTGATGACGC TGGACTTCCT GGGAAAGTAC AACAAGTGGG ATAAGGTGGA CCCCGCCGAG 
CTGTTTAGCC AGGCCCCGAC GGAGAAGAAG GAGGCCAACC CCAAGCTGAA CATGGTGAAG 
TTCCTGCAGG TGGAGGGCAG GGGCTGCGAC TGCATCGTGC TGTGGCTGGA CTGCGACAAG 
GAGGGCGAGA ACATCTGCTT CGAGGTGCTG GACGCGGTGC TGCCCGTCAT GAACCAGGCC 
CGCAGCGGCG AGCAGACAGT GTTCCGGGCG CGCTTCAGCT CCATCACGGA CACAGACATC 
TGCGCTGCCA TGGCCCGCCT GGGTGAGCCC GACCACAACG AGGCGCTCTC GGTGGACGCG 
CGGCAGGAGC TGGACCTCCG CATCGGCTGC GCCTTCACGA GGTTCCAGAC TAAATACTTC 
CAGGGGAAAT ACGGCAACCT GGACAGCTCG CTCATCTCCT TCGGGCCGTG CCAGACGCCC 
ACGCTGGGGT TCTGCGTGGA GAGGCATGAC AAGATCCAGT CCTTCAAGCC AGAGACCTAC 
TGGGTGCTAC AGGCCAAGGT GGATGTTGAT AAGGACCGCT CCCTCCTCCT GGACTGGGAC 
CGTGTGCGCG TGTTCGACCG GGAGATCGCA CAGATGTTCC TGAACATGAC CAAGCTCGAG 
AAGGAGGCCC AGGTGGAGGC CACAAGCAGG AAGGAGAAGG CCAAGCAGCG GCCGCTGGCC 
CTGAACACCG TGGAGATGCT GCGTGTGGCC AGCTCAGCCC TGGGAATGGG CCCGCAGCAC 
GCCATGCAGA CCGCCGAGCG GCTCTACACG CAGGGCTACA TCAGCTACCC GCGCACCGAG 
ACCACCCACT ACCCCGAGAG CTTCGACCTG AAGGGGCCTC TGCGGCAGCA GGCCAACCAC 
CCCTACTGGG CTGACACGGT GAAGCGGCTC TTAGCAGAAG GTATCAACCG CCCACGGAAA 
GGCCATGATG CTGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC GGAGGCCGAG 
TTAGGGGGCG AGGCTTGGCG GCTGTACGAA TACATCACCC GGCACTTCAT CGCCACCGTC 
AGCCACGACT GCAGGTACCT GCAGAGCAGC GTCTCCTTCC GGATCGGGCC CGAGCGCTTC 
ACCTGCACGG GCAAGGCCGT CATCTCCCCA GGCTTCACGG AGATCATGCC CTGGCAGAGC 
GTGCCCCTGG AGGAGAGCCT GCCCACCTGC CAGAAGGGCG ACACCCTGGC CGTGGCTGAG 
GTGAAGCTGC TGGAGAAGCA GACGAGCCCG CCAGACTACC TGACCGAGGC TGAGCTCATC 
ACCCTCATGG AGAAGCACGG CATCGGGACG GACGCCAGCA TCCCCGTGCA CATCAACAAC 
ATCTGCCAGC GCAACTACGT GATCGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTG 
GGCATCGTCC TGGTGCATGG CTACTACAAG ATCGATGCCG AGCTGGTGCT GCCCACCATC 
CGCAGCGCCG TGGAGAAGCA GCTGAACCTG ATCGCACAGG GCAGAGCCGA CTTCCGCCAA 
GTCCTGGGCC ACACCCTGGA CGTCTTCAAG AGGAAGTTCC ACTACTTCGT GGACTCCATC 
GCGGGCATGG ATGAACTGAT GGAGGTGTCC TTCTCGCCCC TGGCGGCCAC GGGCAAGCCC 
CTCTCCCGCT GCGGGAAGTG CCACCGGTTC ATGAAGTACA TCCAGGCCAA GCCGAGCCGC 
CTGCACTGCT CGCACTGCGA CGAGACCTAC ACGCTGCCCC AGAACGGCAC CATCAAGCTC 
TACAAGGAGC TGCGGTGCCC GCTGGACGAC TTCGAGCTGG TCCTGTGGTC GTCGGGCTCC 
AGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTCCAACC ACCCGCCCTT CCGGGACATG 
AAGAAGGGCA CAGGCTGCAA CGAGTGCACG CACCCCAGCT GCCAGCACTC GCTCAGCATG 
CTGGGCATCG GCCAGTGCGT GGAGTGCGAG AGCGGCGTGC TGGTGCTCGA CCCCACCTCC 
GGCCCCAAGT GGCGCGTGGC CTGCAACAGG TGCAACGTGG TGGCCCGCTG CTTCGAGAAC 
GCCCACCGCG TGCGCGTGTC CGCCGAGACG TGCGCCGCCT GCGAGGCAGC CCTGCTGGAC 
GTGGACTTCA ACAAGGCCAA GTCCCCGCTG CCGGGCGACG GCACGCAGCA CACGGGCTGC 
GTCTTCTGCG ACCCGGTCTT CCAGGAGCTG GTGGAGCTGA AGCACGCAGC CTCCTGCCAC 
CCCATGCACC GCGGGCCTGG GCGGAGGCAG GGCCGGGGCC GGGGCCGGGG CCGGAGGCCG 
GCGGGGAAGC CGGGTCCGCG CCGGCCCAAG GACAAGATGT CAGCTCTGGC CGCCTACTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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TOP3B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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