Top3beta cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Top3beta
Entrez Gene ID	31565
Full Name	Topoisomerase 3beta
Synonyms	CG3458,Dmel\CG3458,TOP3,Top3,Topo IIIbeta,dTop3beta,top3,top3[beta],top3beta,topo III[beta]
General protein information	Preferred Names Topoisomerase 3beta Names CG3458 gene product from transcript CG3458-RB CG3458-PA CG3458-PB Top 3beta Top3beta-PA Top3beta-PB topoisomerase 3 topoisomerase 3beta
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 5E5-5E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_078504.4	NP_511059.2	topoisomerase 3beta, isoform A
NM_001298009.1	NP_001284938.1	topoisomerase 3beta, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TOP3B	XP_515006.3
Rattus norvegicus (Norway rat)	Top3b	NP_001099331.1
Mus musculus (house mouse)	Top3b	NP_035754.1
Gallus gallus (chicken)	TOP3B	NP_001006181.1
Drosophila melanogaster (fruit fly)	Top3beta	NP_511059.2
Caenorhabditis elegans (roundworm)	Y48C3A.14	NP_001254381.1
Oryza sativa (rice)	Os09g0500600	NP_001063578.1
Homo sapiens (human)	TOP3B	NP_003926.1
Xenopus tropicalis (tropical clawed frog)	top3b	NP_001182501.1
	TOP3B	XP_001088462.2
Bos taurus (cattle)	TOP3B	NP_001193635.1
Danio rerio (zebrafish)	LOC793414	XP_003198651.1
Arabidopsis thaliana (thale cress)	AT2G32000	NP_180760.2
Canis lupus familiaris (dog)	TOP3B	XP_005636690.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results of this study suggested that Top3beta acts as an RNA topoisomerase and works with FMRP to promote the expression of mRNAs that are crucial for synapse formation.
Title: Top3? is an RNA topoisomerase that works with fragile X syndrome protein to promote synapse formation.

studies suggest that the topo III enzyme behaves as a structure-specific endonuclease in vivo, providing a reversible DNA cleavage activity that is specific for unpaired regions in the DNA
Title: Preferential cleavage of plasmid-based R-loops and D-loops by Drosophila topoisomerase IIIbeta.

The following Top3beta gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Top3beta cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03123	NM_078504.4 Latest version!	Drosophila melanogaster topoisomerase 3beta, transcript variant A (Top3beta), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa03123	NM_001298009.1 Latest version!	Drosophila melanogaster topoisomerase 3beta, transcript variant B (Top3beta), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Top3beta ( NM_078504.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511059.2 Drosophila melanogaster topoisomerase 3beta, transcript variant A (Top3beta), mRNA.

Price & Availability↑

CloneID	OFa03123
Clone ID Related Accession (Same CDS sequence)	NM_078504.4 , NM_001298009.1
Accession Version	NM_078504.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase 3beta, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078504.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAGCG TGCTGATGGT GGCCGAGAAG CCCTCGCTGG CGGCCTCGCT GGCGGGGATT 
CTCTCAAACG GACGCTGCAC GGCCAAGCGT GGCACCGGCA ACGGCTGCTC CACGCACGAG 
TGGACGGGCA ACTTTCGGAA CGAGGGCAGC GTCCACTTCC GGATGACTTC CGTGTGCGGT 
CATGTCATGT CGCTGGACTT CAATAAGAAG TACAACTGCT GGGACAAGGT GGATCCCATC 
CAGCTCTTCG GTTGTGCCAC CGAAAAGAAG GAAACGAATC CCAAGCAGAA CATGCGCAAA 
TTTCTGGCCC ACGAGGCGCG CGGCTGTGAC TATCTGGTGC TCTGGCTGGA TTGCGACAAG 
GAGGGCGAGA ACATCTGCTT CGAGGTGATG GACGCCGTGA AGCATGTCAT CAACAATGTG 
TACAGCGATC AGGTGACATA CCGTGCCCAC TTCTCGGCTA TCACGGAGAA GGACATCAAG 
AAAGCCATGG AGACGTTGGG GCATCCCAAC GAGAACGAGG CCAAGTCGGT GGACGCCCGG 
CAGGAATTGG ACCTGCGCAT TGGCTGCGCC TTTACCCGCT TTCAAACGAA GTTCTTTCAG 
GATCGCTACG GTGACCTGGA TTCGTCGCTG ATCTCCTACG GTCCCTGCCA GACGCCCACG 
CTCGGTTTCT GCGTGAAGCG GCACGACGAC ATCCAGACCT TTAAGCCGGA GAGCTTCTGG 
CATCTGCAGC TCCTCGCCGG CCAGCCGGAA GTCACGCTTG AATGGGCGCG CGGTCGGGTC 
TTCAAGAAGG ACATCGCCAT CATGCTGCTG AATCGCGTCA AGGAGCACAA GAAGGCCACT 
GTGGAGAGCG TGGCCAGCAA GGAGGCCTAC AAGAGCAAAC CACAGGCACT GAACACCGTC 
GAACTGATGC GTATCTGCAG CTCCGGACTG GGCATCGGAC CCTTCCAGGC GATGCAGATA 
GCTGAGCGCC TGTACACGCA GGGCTACATC AGCTATCCGC GAACAGAGAC CAATCAGTAT 
CCGACTAACT TCGATCTGCC GGCCGTGCTG CACGTGCTGA AGCCATCGGC GGACTTCGGT 
GAGGAGGCGC GCTCCATTTT GGGCGACATT CAGACGCCGC GAAAGGGCAA GGATGCCGGC 
GACCATCCAC CCATCACGCC AATGAAACTG GGCAACCGCA GTGACTTCGA TCGCGACACC 
TGGCGTGTCT ACGAGTTCAT CTGCCGGCAC TTCATGGGCA CGGTGTCGAG GGATCTTAAG 
TACCGCGTGA CGACGGCAAA GCTGAGTGTT GGCATGGAGA CATTCAGTTG CACGGCCAGT 
GTGCTGATCG ATGCCGGTTT CACCAAGGTG ATGACGTGGT CGGCCTTCGG CAAGGACGAA 
CCCCAGCCGC CGTTCGTTCA GGGCACTCAG GTGGCCATCA ACGATGTGCG ACTTATCGAA 
AGCCAGACAG GACCGCCGGA TTATCTCACC GAATCGGAGC TAATCACACT GATGGAGGAG 
CACGGCATCG GGACGGATGC CTCCATACCG GTGCACATCA ACAACATCTG CCAGCGCAAC 
TATGTGCACA TTGAGAACGG TCGCAAGCTG ATGCCAACGA CGCTGGGCAT TGTGCTGGTC 
CACGGCTATC AGAAGATCGA TCCGGAACTG GTGCTGCCCA CCATGCGAAC GGAGGTGGAG 
CGCATGCTGA CACTGATTGC CCAGGGATCG GCTAACTTTC AGGATGTTCT GCGACACGCC 
ATCAAGATTT TCAAGCTGAA GTTTATGTAC TTTGTGAAGA ACATCGACAG CATGGACGCA 
CTGTTTGAGG TATCCTTCTC GCCGCTGGCC GAGTCGGGCA AGGCGCACTC GCGCTGCGGC 
AAGTGTAGGC GCTACATGAA GTACATACAG ACCAAACCGG CGAGATTGCA CTGCTCTCAT 
TGCGATGAGA CATACGCCCT GCCCATTGGC AATGTGAAGG TGTATCGCGA GTTCAAGTGC 
CCGCTGGACG ACTTTGATCT GCTGGCTTTC TCCACTGGCG TGAAGGGGCG TTCGTATCCG 
TTCTGCCCGT ACTGTTACAA CCATCCGCCG TTCAGCGACA TGCCCCACTT GGGGGGCTGC 
AACACCTGCA CGAACGCCAA CTGCCCGCAC TCGCTGAACA CGCTGGGCAT CTCCAGCTGC 
GTGGAGTGCC CCACCGGGGT CCTGGTGCTC GATTGTACGC TTGCGCCAAC CTGGAAGCTG 
GGCTGCAATC GCTGCGACGT GATCATCAAC TGCTTCAAGG GAGCCACCAA GATCACGGTG 
GAAGAGGCCA AGTGCCAGGA GTGTGGCGCC CAGCAGGTGA ATGTGGTCTA CAAGTCGGAC 
AAGAGCAAGT TCAAGGACGG AAGCGAGGAG AAGAGCGGCT GCATCTTCTG CTCCGCAGAC 
TTCTCGCACT TGGTCGAGAA GCATCGGGCG GTGGCATCCA GACCAGTTCG CAGTGGCGGC 
GGCTTCCGTG GCGGCAAGGC AGGTCGAGGC GGTGGCGGAA TGGGCGGGGC GGCTTTTGGC 
TCCGGAGGAG CGGTTACGGC TGGCGGTGGA CCTAATGCTG GGGGCGGGGT GCGTGGCAGT 
CGAGTGGCCA AGGATAAGAT GGGACAACTG GCGTCGTATT TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511059.2
CDS	260..2887
Translation	MKSVLMVAEKPSLAASLAGILSNGRCTAKRGTGNGCSTHEWTGNFRNEGSVHFRMTSVCGHVMSLDFNKKYNCWDKVDPIQLFGCATEKKETNPKQNMRKFLAHEARGCDYLVLWLDCDKEGENICFEVMDAVKHVINNVYSDQVTYRAHFSAITEKDIKKAMETLGHPNENEAKSVDARQELDLRIGCAFTRFQTKFFQDRYGDLDSSLISYGPCQTPTLGFCVKRHDDIQTFKPESFWHLQLLAGQPEVTLEWARGRVFKKDIAIMLLNRVKEHKKATVESVASKEAYKSKPQALNTVELMRICSSGLGIGPFQAMQIAERLYTQGYISYPRTETNQYPTNFDLPAVLHVLKPSADFGEEARSILGDIQTPRKGKDAGDHPPITPMKLGNRSDFDRDTWRVYEFICRHFMGTVSRDLKYRVTTAKLSVGMETFSCTASVLIDAGFTKVMTWSAFGKDEPQPPFVQGTQVAINDVRLIESQTGPPDYLTESELITLMEEHGIGTDASIPVHINNICQRNYVHIENGRKLMPTTLGIVLVHGYQKIDPELVLPTMRTEVERMLTLIAQGSANFQDVLRHAIKIFKLKFMYFVKNIDSMDALFEVSFSPLAESGKAHSRCGKCRRYMKYIQTKPARLHCSHCDETYALPIGNVKVYREFKCPLDDFDLLAFSTGVKGRSYPFCPYCYNHPPFSDMPHLGGCNTCTNANCPHSLNTLGISSCVECPTGVLVLDCTLAPTWKLGCNRCDVIINCFKGATKITVEEAKCQECGAQQVNVVYKSDKSKFKDGSEEKSGCIFCSADFSHLVEKHRAVASRPVRSGGGFRGGKAGRGGGGMGGAAFGSGGAVTAGGGPNAGGGVRGSRVAKDKMGQLASYFV

Target ORF information:

RefSeq Version	NM_078504.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase 3beta, transcript variant A (Top3beta), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078504.4

ORF Insert Sequence:

ATGAAGAGCG TGCTGATGGT GGCCGAGAAG CCCTCGCTGG CGGCCTCGCT GGCGGGGATT 
CTCTCAAACG GACGCTGCAC GGCCAAGCGT GGCACCGGCA ACGGCTGCTC CACGCACGAG 
TGGACGGGCA ACTTTCGGAA CGAGGGCAGC GTCCACTTCC GGATGACTTC CGTGTGCGGT 
CATGTCATGT CGCTGGACTT CAATAAGAAG TACAACTGCT GGGACAAGGT GGATCCCATC 
CAGCTCTTCG GTTGTGCCAC CGAAAAGAAG GAAACGAATC CCAAGCAGAA CATGCGCAAA 
TTTCTGGCCC ACGAGGCGCG CGGCTGTGAC TATCTGGTGC TCTGGCTGGA TTGCGACAAG 
GAGGGCGAGA ACATCTGCTT CGAGGTGATG GACGCCGTGA AGCATGTCAT CAACAATGTG 
TACAGCGATC AGGTGACATA CCGTGCCCAC TTCTCGGCTA TCACGGAGAA GGACATCAAG 
AAAGCCATGG AGACGTTGGG GCATCCCAAC GAGAACGAGG CCAAGTCGGT GGACGCCCGG 
CAGGAATTGG ACCTGCGCAT TGGCTGCGCC TTTACCCGCT TTCAAACGAA GTTCTTTCAG 
GATCGCTACG GTGACCTGGA TTCGTCGCTG ATCTCCTACG GTCCCTGCCA GACGCCCACG 
CTCGGTTTCT GCGTGAAGCG GCACGACGAC ATCCAGACCT TTAAGCCGGA GAGCTTCTGG 
CATCTGCAGC TCCTCGCCGG CCAGCCGGAA GTCACGCTTG AATGGGCGCG CGGTCGGGTC 
TTCAAGAAGG ACATCGCCAT CATGCTGCTG AATCGCGTCA AGGAGCACAA GAAGGCCACT 
GTGGAGAGCG TGGCCAGCAA GGAGGCCTAC AAGAGCAAAC CACAGGCACT GAACACCGTC 
GAACTGATGC GTATCTGCAG CTCCGGACTG GGCATCGGAC CCTTCCAGGC GATGCAGATA 
GCTGAGCGCC TGTACACGCA GGGCTACATC AGCTATCCGC GAACAGAGAC CAATCAGTAT 
CCGACTAACT TCGATCTGCC GGCCGTGCTG CACGTGCTGA AGCCATCGGC GGACTTCGGT 
GAGGAGGCGC GCTCCATTTT GGGCGACATT CAGACGCCGC GAAAGGGCAA GGATGCCGGC 
GACCATCCAC CCATCACGCC AATGAAACTG GGCAACCGCA GTGACTTCGA TCGCGACACC 
TGGCGTGTCT ACGAGTTCAT CTGCCGGCAC TTCATGGGCA CGGTGTCGAG GGATCTTAAG 
TACCGCGTGA CGACGGCAAA GCTGAGTGTT GGCATGGAGA CATTCAGTTG CACGGCCAGT 
GTGCTGATCG ATGCCGGTTT CACCAAGGTG ATGACGTGGT CGGCCTTCGG CAAGGACGAA 
CCCCAGCCGC CGTTCGTTCA GGGCACTCAG GTGGCCATCA ACGATGTGCG ACTTATCGAA 
AGCCAGACAG GACCGCCGGA TTATCTCACC GAATCGGAGC TAATCACACT GATGGAGGAG 
CACGGCATCG GGACGGATGC CTCCATACCG GTGCACATCA ACAACATCTG CCAGCGCAAC 
TATGTGCACA TTGAGAACGG TCGCAAGCTG ATGCCAACGA CGCTGGGCAT TGTGCTGGTC 
CACGGCTATC AGAAGATCGA TCCGGAACTG GTGCTGCCCA CCATGCGAAC GGAGGTGGAG 
CGCATGCTGA CACTGATTGC CCAGGGATCG GCTAACTTTC AGGATGTTCT GCGACACGCC 
ATCAAGATTT TCAAGCTGAA GTTTATGTAC TTTGTGAAGA ACATCGACAG CATGGACGCA 
CTGTTTGAGG TATCCTTCTC GCCGCTGGCC GAGTCGGGCA AGGCGCACTC GCGCTGCGGC 
AAGTGTAGGC GCTACATGAA GTACATACAG ACCAAACCGG CGAGATTGCA CTGCTCTCAT 
TGCGATGAGA CATACGCCCT GCCCATTGGC AATGTGAAGG TGTATCGCGA GTTCAAGTGC 
CCGCTGGACG ACTTTGATCT GCTGGCTTTC TCCACTGGCG TGAAGGGGCG TTCGTATCCG 
TTCTGCCCGT ACTGTTACAA CCATCCGCCG TTCAGCGACA TGCCCCACTT GGGGGGCTGC 
AACACCTGCA CGAACGCCAA CTGCCCGCAC TCGCTGAACA CGCTGGGCAT CTCCAGCTGC 
GTGGAGTGCC CCACCGGGGT CCTGGTGCTC GATTGTACGC TTGCGCCAAC CTGGAAGCTG 
GGCTGCAATC GCTGCGACGT GATCATCAAC TGCTTCAAGG GAGCCACCAA GATCACGGTG 
GAAGAGGCCA AGTGCCAGGA GTGTGGCGCC CAGCAGGTGA ATGTGGTCTA CAAGTCGGAC 
AAGAGCAAGT TCAAGGACGG AAGCGAGGAG AAGAGCGGCT GCATCTTCTG CTCCGCAGAC 
TTCTCGCACT TGGTCGAGAA GCATCGGGCG GTGGCATCCA GACCAGTTCG CAGTGGCGGC 
GGCTTCCGTG GCGGCAAGGC AGGTCGAGGC GGTGGCGGAA TGGGCGGGGC GGCTTTTGGC 
TCCGGAGGAG CGGTTACGGC TGGCGGTGGA CCTAATGCTG GGGGCGGGGT GCGTGGCAGT 
CGAGTGGCCA AGGATAAGAT GGGACAACTG GCGTCGTATT TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Top3beta ( NM_001298009.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284938.1 Drosophila melanogaster topoisomerase 3beta, transcript variant B (Top3beta), mRNA.

Price & Availability↑

CloneID	OFa03123
Clone ID Related Accession (Same CDS sequence)	NM_078504.4 , NM_001298009.1
Accession Version	NM_001298009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase 3beta, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAGCG TGCTGATGGT GGCCGAGAAG CCCTCGCTGG CGGCCTCGCT GGCGGGGATT 
CTCTCAAACG GACGCTGCAC GGCCAAGCGT GGCACCGGCA ACGGCTGCTC CACGCACGAG 
TGGACGGGCA ACTTTCGGAA CGAGGGCAGC GTCCACTTCC GGATGACTTC CGTGTGCGGT 
CATGTCATGT CGCTGGACTT CAATAAGAAG TACAACTGCT GGGACAAGGT GGATCCCATC 
CAGCTCTTCG GTTGTGCCAC CGAAAAGAAG GAAACGAATC CCAAGCAGAA CATGCGCAAA 
TTTCTGGCCC ACGAGGCGCG CGGCTGTGAC TATCTGGTGC TCTGGCTGGA TTGCGACAAG 
GAGGGCGAGA ACATCTGCTT CGAGGTGATG GACGCCGTGA AGCATGTCAT CAACAATGTG 
TACAGCGATC AGGTGACATA CCGTGCCCAC TTCTCGGCTA TCACGGAGAA GGACATCAAG 
AAAGCCATGG AGACGTTGGG GCATCCCAAC GAGAACGAGG CCAAGTCGGT GGACGCCCGG 
CAGGAATTGG ACCTGCGCAT TGGCTGCGCC TTTACCCGCT TTCAAACGAA GTTCTTTCAG 
GATCGCTACG GTGACCTGGA TTCGTCGCTG ATCTCCTACG GTCCCTGCCA GACGCCCACG 
CTCGGTTTCT GCGTGAAGCG GCACGACGAC ATCCAGACCT TTAAGCCGGA GAGCTTCTGG 
CATCTGCAGC TCCTCGCCGG CCAGCCGGAA GTCACGCTTG AATGGGCGCG CGGTCGGGTC 
TTCAAGAAGG ACATCGCCAT CATGCTGCTG AATCGCGTCA AGGAGCACAA GAAGGCCACT 
GTGGAGAGCG TGGCCAGCAA GGAGGCCTAC AAGAGCAAAC CACAGGCACT GAACACCGTC 
GAACTGATGC GTATCTGCAG CTCCGGACTG GGCATCGGAC CCTTCCAGGC GATGCAGATA 
GCTGAGCGCC TGTACACGCA GGGCTACATC AGCTATCCGC GAACAGAGAC CAATCAGTAT 
CCGACTAACT TCGATCTGCC GGCCGTGCTG CACGTGCTGA AGCCATCGGC GGACTTCGGT 
GAGGAGGCGC GCTCCATTTT GGGCGACATT CAGACGCCGC GAAAGGGCAA GGATGCCGGC 
GACCATCCAC CCATCACGCC AATGAAACTG GGCAACCGCA GTGACTTCGA TCGCGACACC 
TGGCGTGTCT ACGAGTTCAT CTGCCGGCAC TTCATGGGCA CGGTGTCGAG GGATCTTAAG 
TACCGCGTGA CGACGGCAAA GCTGAGTGTT GGCATGGAGA CATTCAGTTG CACGGCCAGT 
GTGCTGATCG ATGCCGGTTT CACCAAGGTG ATGACGTGGT CGGCCTTCGG CAAGGACGAA 
CCCCAGCCGC CGTTCGTTCA GGGCACTCAG GTGGCCATCA ACGATGTGCG ACTTATCGAA 
AGCCAGACAG GACCGCCGGA TTATCTCACC GAATCGGAGC TAATCACACT GATGGAGGAG 
CACGGCATCG GGACGGATGC CTCCATACCG GTGCACATCA ACAACATCTG CCAGCGCAAC 
TATGTGCACA TTGAGAACGG TCGCAAGCTG ATGCCAACGA CGCTGGGCAT TGTGCTGGTC 
CACGGCTATC AGAAGATCGA TCCGGAACTG GTGCTGCCCA CCATGCGAAC GGAGGTGGAG 
CGCATGCTGA CACTGATTGC CCAGGGATCG GCTAACTTTC AGGATGTTCT GCGACACGCC 
ATCAAGATTT TCAAGCTGAA GTTTATGTAC TTTGTGAAGA ACATCGACAG CATGGACGCA 
CTGTTTGAGG TATCCTTCTC GCCGCTGGCC GAGTCGGGCA AGGCGCACTC GCGCTGCGGC 
AAGTGTAGGC GCTACATGAA GTACATACAG ACCAAACCGG CGAGATTGCA CTGCTCTCAT 
TGCGATGAGA CATACGCCCT GCCCATTGGC AATGTGAAGG TGTATCGCGA GTTCAAGTGC 
CCGCTGGACG ACTTTGATCT GCTGGCTTTC TCCACTGGCG TGAAGGGGCG TTCGTATCCG 
TTCTGCCCGT ACTGTTACAA CCATCCGCCG TTCAGCGACA TGCCCCACTT GGGGGGCTGC 
AACACCTGCA CGAACGCCAA CTGCCCGCAC TCGCTGAACA CGCTGGGCAT CTCCAGCTGC 
GTGGAGTGCC CCACCGGGGT CCTGGTGCTC GATTGTACGC TTGCGCCAAC CTGGAAGCTG 
GGCTGCAATC GCTGCGACGT GATCATCAAC TGCTTCAAGG GAGCCACCAA GATCACGGTG 
GAAGAGGCCA AGTGCCAGGA GTGTGGCGCC CAGCAGGTGA ATGTGGTCTA CAAGTCGGAC 
AAGAGCAAGT TCAAGGACGG AAGCGAGGAG AAGAGCGGCT GCATCTTCTG CTCCGCAGAC 
TTCTCGCACT TGGTCGAGAA GCATCGGGCG GTGGCATCCA GACCAGTTCG CAGTGGCGGC 
GGCTTCCGTG GCGGCAAGGC AGGTCGAGGC GGTGGCGGAA TGGGCGGGGC GGCTTTTGGC 
TCCGGAGGAG CGGTTACGGC TGGCGGTGGA CCTAATGCTG GGGGCGGGGT GCGTGGCAGT 
CGAGTGGCCA AGGATAAGAT GGGACAACTG GCGTCGTATT TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284938.1
CDS	260..2887
Translation	MKSVLMVAEKPSLAASLAGILSNGRCTAKRGTGNGCSTHEWTGNFRNEGSVHFRMTSVCGHVMSLDFNKKYNCWDKVDPIQLFGCATEKKETNPKQNMRKFLAHEARGCDYLVLWLDCDKEGENICFEVMDAVKHVINNVYSDQVTYRAHFSAITEKDIKKAMETLGHPNENEAKSVDARQELDLRIGCAFTRFQTKFFQDRYGDLDSSLISYGPCQTPTLGFCVKRHDDIQTFKPESFWHLQLLAGQPEVTLEWARGRVFKKDIAIMLLNRVKEHKKATVESVASKEAYKSKPQALNTVELMRICSSGLGIGPFQAMQIAERLYTQGYISYPRTETNQYPTNFDLPAVLHVLKPSADFGEEARSILGDIQTPRKGKDAGDHPPITPMKLGNRSDFDRDTWRVYEFICRHFMGTVSRDLKYRVTTAKLSVGMETFSCTASVLIDAGFTKVMTWSAFGKDEPQPPFVQGTQVAINDVRLIESQTGPPDYLTESELITLMEEHGIGTDASIPVHINNICQRNYVHIENGRKLMPTTLGIVLVHGYQKIDPELVLPTMRTEVERMLTLIAQGSANFQDVLRHAIKIFKLKFMYFVKNIDSMDALFEVSFSPLAESGKAHSRCGKCRRYMKYIQTKPARLHCSHCDETYALPIGNVKVYREFKCPLDDFDLLAFSTGVKGRSYPFCPYCYNHPPFSDMPHLGGCNTCTNANCPHSLNTLGISSCVECPTGVLVLDCTLAPTWKLGCNRCDVIINCFKGATKITVEEAKCQECGAQQVNVVYKSDKSKFKDGSEEKSGCIFCSADFSHLVEKHRAVASRPVRSGGGFRGGKAGRGGGGMGGAAFGSGGAVTAGGGPNAGGGVRGSRVAKDKMGQLASYFV

Target ORF information:

RefSeq Version	NM_001298009.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase 3beta, transcript variant B (Top3beta), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298009.1

ORF Insert Sequence:

ATGAAGAGCG TGCTGATGGT GGCCGAGAAG CCCTCGCTGG CGGCCTCGCT GGCGGGGATT 
CTCTCAAACG GACGCTGCAC GGCCAAGCGT GGCACCGGCA ACGGCTGCTC CACGCACGAG 
TGGACGGGCA ACTTTCGGAA CGAGGGCAGC GTCCACTTCC GGATGACTTC CGTGTGCGGT 
CATGTCATGT CGCTGGACTT CAATAAGAAG TACAACTGCT GGGACAAGGT GGATCCCATC 
CAGCTCTTCG GTTGTGCCAC CGAAAAGAAG GAAACGAATC CCAAGCAGAA CATGCGCAAA 
TTTCTGGCCC ACGAGGCGCG CGGCTGTGAC TATCTGGTGC TCTGGCTGGA TTGCGACAAG 
GAGGGCGAGA ACATCTGCTT CGAGGTGATG GACGCCGTGA AGCATGTCAT CAACAATGTG 
TACAGCGATC AGGTGACATA CCGTGCCCAC TTCTCGGCTA TCACGGAGAA GGACATCAAG 
AAAGCCATGG AGACGTTGGG GCATCCCAAC GAGAACGAGG CCAAGTCGGT GGACGCCCGG 
CAGGAATTGG ACCTGCGCAT TGGCTGCGCC TTTACCCGCT TTCAAACGAA GTTCTTTCAG 
GATCGCTACG GTGACCTGGA TTCGTCGCTG ATCTCCTACG GTCCCTGCCA GACGCCCACG 
CTCGGTTTCT GCGTGAAGCG GCACGACGAC ATCCAGACCT TTAAGCCGGA GAGCTTCTGG 
CATCTGCAGC TCCTCGCCGG CCAGCCGGAA GTCACGCTTG AATGGGCGCG CGGTCGGGTC 
TTCAAGAAGG ACATCGCCAT CATGCTGCTG AATCGCGTCA AGGAGCACAA GAAGGCCACT 
GTGGAGAGCG TGGCCAGCAA GGAGGCCTAC AAGAGCAAAC CACAGGCACT GAACACCGTC 
GAACTGATGC GTATCTGCAG CTCCGGACTG GGCATCGGAC CCTTCCAGGC GATGCAGATA 
GCTGAGCGCC TGTACACGCA GGGCTACATC AGCTATCCGC GAACAGAGAC CAATCAGTAT 
CCGACTAACT TCGATCTGCC GGCCGTGCTG CACGTGCTGA AGCCATCGGC GGACTTCGGT 
GAGGAGGCGC GCTCCATTTT GGGCGACATT CAGACGCCGC GAAAGGGCAA GGATGCCGGC 
GACCATCCAC CCATCACGCC AATGAAACTG GGCAACCGCA GTGACTTCGA TCGCGACACC 
TGGCGTGTCT ACGAGTTCAT CTGCCGGCAC TTCATGGGCA CGGTGTCGAG GGATCTTAAG 
TACCGCGTGA CGACGGCAAA GCTGAGTGTT GGCATGGAGA CATTCAGTTG CACGGCCAGT 
GTGCTGATCG ATGCCGGTTT CACCAAGGTG ATGACGTGGT CGGCCTTCGG CAAGGACGAA 
CCCCAGCCGC CGTTCGTTCA GGGCACTCAG GTGGCCATCA ACGATGTGCG ACTTATCGAA 
AGCCAGACAG GACCGCCGGA TTATCTCACC GAATCGGAGC TAATCACACT GATGGAGGAG 
CACGGCATCG GGACGGATGC CTCCATACCG GTGCACATCA ACAACATCTG CCAGCGCAAC 
TATGTGCACA TTGAGAACGG TCGCAAGCTG ATGCCAACGA CGCTGGGCAT TGTGCTGGTC 
CACGGCTATC AGAAGATCGA TCCGGAACTG GTGCTGCCCA CCATGCGAAC GGAGGTGGAG 
CGCATGCTGA CACTGATTGC CCAGGGATCG GCTAACTTTC AGGATGTTCT GCGACACGCC 
ATCAAGATTT TCAAGCTGAA GTTTATGTAC TTTGTGAAGA ACATCGACAG CATGGACGCA 
CTGTTTGAGG TATCCTTCTC GCCGCTGGCC GAGTCGGGCA AGGCGCACTC GCGCTGCGGC 
AAGTGTAGGC GCTACATGAA GTACATACAG ACCAAACCGG CGAGATTGCA CTGCTCTCAT 
TGCGATGAGA CATACGCCCT GCCCATTGGC AATGTGAAGG TGTATCGCGA GTTCAAGTGC 
CCGCTGGACG ACTTTGATCT GCTGGCTTTC TCCACTGGCG TGAAGGGGCG TTCGTATCCG 
TTCTGCCCGT ACTGTTACAA CCATCCGCCG TTCAGCGACA TGCCCCACTT GGGGGGCTGC 
AACACCTGCA CGAACGCCAA CTGCCCGCAC TCGCTGAACA CGCTGGGCAT CTCCAGCTGC 
GTGGAGTGCC CCACCGGGGT CCTGGTGCTC GATTGTACGC TTGCGCCAAC CTGGAAGCTG 
GGCTGCAATC GCTGCGACGT GATCATCAAC TGCTTCAAGG GAGCCACCAA GATCACGGTG 
GAAGAGGCCA AGTGCCAGGA GTGTGGCGCC CAGCAGGTGA ATGTGGTCTA CAAGTCGGAC 
AAGAGCAAGT TCAAGGACGG AAGCGAGGAG AAGAGCGGCT GCATCTTCTG CTCCGCAGAC 
TTCTCGCACT TGGTCGAGAA GCATCGGGCG GTGGCATCCA GACCAGTTCG CAGTGGCGGC 
GGCTTCCGTG GCGGCAAGGC AGGTCGAGGC GGTGGCGGAA TGGGCGGGGC GGCTTTTGGC 
TCCGGAGGAG CGGTTACGGC TGGCGGTGGA CCTAATGCTG GGGGCGGGGT GCGTGGCAGT 
CGAGTGGCCA AGGATAAGAT GGGACAACTG GCGTCGTATT TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Top3beta cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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