MED25 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED25
Entrez Gene ID	81857
Full Name	mediator complex subunit 25
Synonyms	ACID1,ARC92,BVSYS,CMT2B2,P78,PTOV2,TCBAP0758
General protein information	Preferred Names mediator complex subunit 25 Names mediator of RNA polymerase II transcription subunit 25 ARC/mediator transcriptional coactivator subunit activator interaction domain-containing protein 1 activator-recruited cofactor 92 kDa component mediator of RNA polymerase II transcription, subunit 25 homolog prostate-derived protein 78 kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.33
Summary	This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq, Apr 2010].
Disorder MIM:	610197
Disorder Html:	?Charcot-Marie-Tooth disease, type 2B2, 605589 (3); Basel-Vanagait-Smirin-Yosef syndrome, 616449 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011527353.1	XP_011525655.1	mediator of RNA polymerase II transcription subunit 25 isoform X1
NM_030973.3	NP_112235.2	mediator of RNA polymerase II transcription subunit 25

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MED25	XP_005616347.1
Pan troglodytes (chimpanzee)	MED25	XP_003316596.2
Mus musculus (house mouse)	Med25	NP_083641.1
Rattus norvegicus (Norway rat)	Med25	NP_001163897.1
Bos taurus (cattle)	MED25	NP_001075914.1
Homo sapiens (human)	MED25	NP_112235.2
	MED25	XP_001115505.1
Xenopus tropicalis (tropical clawed frog)	med25	NP_001096266.1

Gene Ontology
Bibliography

Related articles in PubMed

ETV4 and AP1 Transcription Factors Form Multivalent Interactions with three Sites on the MED25 Activator-Interacting Domain.
Currie SL, Doane JJ, Evans KS, Bhachech N, Madison BJ, Lau DKW, McIntosh LP, Skalicky JJ, Clark KA, Graves BJ
Journal of molecular biology429(20)2975-2995(2017 Oct)

Upregulation of human CYP2C9 expression by Bisphenol A via estrogen receptor alpha (ER?) and Med25.
Xu JY, Wu L, Shi Z, Zhang XJ, Englert NA, Zhang SY
Environmental toxicology32(3)970-978(2017 Mar)

Frameshift Mutation of MED25, a Transcription Regulator, and its Mutational Heterogeneity in Colorectal Cancers.
Jo YS, Kim MS, Yoo NJ, Lee SH
Pathology oncology research : POR22(4)875-6(2016 Oct)

Characterization of ERM transactivation domain binding to the ACID/PTOV domain of the Mediator subunit MED25.
Landrieu I, Verger A, Baert JL, Rucktooa P, Cantrelle FX, Dewitte F, Ferreira E, Lens Z, Villeret V, Mont? D
Nucleic acids research43(14)7110-21(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FOS strongly binds to the same MED25 site as ETV4 activation domain and JUN interacts with the other two MED25 sites.
Title: ETV4 and AP1 Transcription Factors Form Multivalent Interactions with three Sites on the MED25 Activator-Interacting Domain.

Med25, a variable member of the Mediator complex, is a coactivator of ligand-activated ERalpha that interacts with ERalpha through its C-terminal LXXLL motif after BPA exposure, and is functionally involved in BPA-induced transcriptional regulation of CYP2C9 expression and enzyme activity.
Title: Upregulation of human CYP2C9 expression by Bisphenol A via estrogen receptor alpha (ER?) and Med25.

In the Title.
Title: Frameshift Mutation of MED25, a Transcription Regulator, and its Mutational Heterogeneity in Colorectal Cancers.

A model is proposed in which the N-terminal transactivation domain of ERM binds the MED25 VP16 H1 binding pocket in a helical conformation.
Title: Characterization of ERM transactivation domain binding to the ACID/PTOV domain of the Mediator subunit MED25.

Homozygous MED25 mutation implicated in eye-intellectual disability syndrome.
Title: Homozygous MED25 mutation implicated in eye-intellectual disability syndrome.

The following MED25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu26796	NM_030973.3 Latest version!	Homo sapiens mediator complex subunit 25 (MED25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu73001	XM_011527353.1 Latest version!	Homo sapiens mediator complex subunit 25 (MED25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED25 ( NM_030973.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_112235.2 Homo sapiens mediator complex subunit 25 (MED25), mRNA.

Price & Availability↑

CloneID	OHu26796
Clone ID Related Accession (Same CDS sequence)	NM_030973.3
Accession Version	NM_030973.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 25
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC410997.1, BC065297.1 and BG770032.1. This sequence is a reference standard in the RefSeqGene project. On Apr 8, 2010 this sequence version replaced NM_030973.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF261072.1, BC065297.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTCCCCG GGTCCGAGGG CCCGGCCCGC GCCGGGAGCG TGGTGGCCGA CGTGGTGTTT 
GTGATTGAGG GTACGGCCAA CCTGGGACCC TACTTCGAGG GGCTCCGCAA GCACTACCTG 
CTCCCGGCCA TCGAGTATTT TAATGGTGGT CCTCCTGCTG AGACGGACTT CGGGGGAGAC 
TATGGGGGGA CCCAGTACAG CCTCGTGGTG TTCAACACAG TGGACTGCGC TCCCGAGTCC 
TACGTACAAT GTCACGCTCC CACCAGCAGC GCCTATGAGT TTGTCACCTG GCTCGATGGC 
ATTAAGTTCA TGGGCGGGGG TGGTGAGAGC TGCAGCCTCA TCGCGGAAGG ACTCAGCACA 
GCCTTGCAGC TGTTTGATGA CTTCAAGAAG ATGCGCGAGC AGATTGGCCA GACGCACCGG 
GTCTGCCTCC TCATCTGCAA CTCACCCCCA TACTTGTTGC CTGCTGTTGA GAGCACCACG 
TACTCTGGAT GCACAACTGA GAATCTTGTG CAGCAGATTG GGGAGCGGGG GATCCACTTC 
TCCATTGTGT CTCCCCGGAA GCTGCCTGCG CTTCGGCTTC TGTTTGAGAA GGCAGCCCCC 
CCGGCCTTGC TGGAGCCGCT GCAGCCTCCG ACAGATGTGA GCCAGGACCC GAGGCACATG 
GTGCTGGTTC GGGGACTCGT GCTGCCTGTT GGGGGTGGCT CAGCCCCAGG CCCCCTCCAG 
TCAAAGCAGC CAGTCCCCCT GCCTCCCGCC GCACCCTCAG GTGCCACTCT CTCAGCAGCC 
CCCCAGCAGC CTCTGCCCCC CGTCCCCCCG CAGTACCAGG TTCCCGGGAA CCTGAGTGCA 
GCTCAGGTGG CCGCGCAGAA TGCAGTGGAG GCTGCCAAGA ACCAGAAGGC TGGGCTGGGC 
CCTCGCTTCT CGCCCATCAC CCCTCTCCAA CAAGCTGCTC CCGGAGTGGG TCCCCCCTTC 
AGCCAGGCCC CAGCTCCCCA ACTACCCCCA GGACCCCCTG GCGCCCCCAA GCCACCACCT 
GCTTCCCAGC CCAGTCTGGT CTCCACTGTG GCCCCTGGCT CCGGCCTGGC TCCCACGGCA 
CAGCCCGGGG CACCGTCCAT GGCAGGCACT GTGGCCCCAG GAGGGGTGAG CGGCCCTTCC 
CCAGCCCAGC TGGGAGCCCC AGCCCTCGGT GGGCAGCAGT CAGTCTCCAA TAAGCTTCTG 
GCCTGGAGCG GGGTCCTGGA GTGGCAAGAG AAACCCAAAC CTGCCTCAGT GGATGCCAAC 
ACCAAGCTGA CGCGGTCACT GCCCTGCCAG GTCTACGTGA ATCATGGCGA GAACCTGAAG 
ACGGAGCAGT GGCCCCAGAA GCTGATCATG CAGCTCATCC CCCAGCAGCT GCTGACCACC 
CTGGGCCCTT TGTTCCGGAA CTCAAGGATG GTCCAGTTCC ATTTCACCAA CAAGGACCTG 
GAGTCTCTCA AAGGCCTCTA CCGCATCATG GGCAACGGCT TCGCGGGCTG CGTGCACTTC 
CCCCACACGG CGCCCTGTGA GGTGCGCGTG CTCATGCTCC TGTACTCGTC CAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAACGGCAT CCGGCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AGCAGCAGCG AGGAATGGGG 
GGACAGCAGG CACCCCCAGG GCTGGGGCCC ATTCTGGAGG ACCAAGCCAG GCCCTCACAG 
AATCTGCTCC AGCTCCGCCC ACCGCAGCCC CAGCCTCAGG GTACCGTAGG GGCCTCTGGG 
GCCACGGGGC AGCCCCAGCC CCAAGGTACT GCCCAGCCCC CGCCAGGTGC CCCTCAAGGC 
CCTCCTGGAG CAGCTTCTGG CCCACCCCCT CCTGGACCCA TCCTTCGGCC CCAGAACCCT 
GGGGCCAACC CTCAGCTGCG AAGCCTCCTC CTCAACCCAC CACCGCCGCA GACTGGGGTG 
CCCCCACCCC AGGCCTCCCT CCACCACCTC CAGCCACCAG GGGCTCCTGC GCTGCTGCCT 
CCGCCGCACC AGGGCCTGGG GCAGCCCCAG TTGGGGCCCC CACTCCTGCA TCCACCACCT 
GCCCAGTCCT GGCCCGCACA ACTTCCCCCT CGGGCTCCAC TGCCAGGTCA GATGCTGCTG 
AGCGGGGGTC CCCGGGGCCC GGTCCCCCAG CCGGGCCTGC AGCCCAGCGT CATGGAGGAC 
GACATCCTCA TGGATCTCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112235.2
CDS	64..2307
Translation	MVPGSEGPARAGSVVADVVFVIEGTANLGPYFEGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTENLVQQIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQPPTDVSQDPRHMVLVRGLVLPVGGGSAPGPLQSKQPVPLPPAAPSGATLSAAPQQPLPPVPPQYQVPGNLSAAQVAAQNAVEAAKNQKAGLGPRFSPITPLQQAAPGVGPPFSQAPAPQLPPGPPGAPKPPPASQPSLVSTVAPGSGLAPTAQPGAPSMAGTVAPGGVSGPSPAQLGAPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQQRGMGGQQAPPGLGPILEDQARPSQNLLQLRPPQPQPQGTVGASGATGQPQPQGTAQPPPGAPQGPPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPGAPALLPPPHQGLGQPQLGPPLLHPPPAQSWPAQLPPRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	NM_030973.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 25 (MED25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030973.3

ORF Insert Sequence:

ATGGTCCCCG GGTCCGAGGG CCCGGCCCGC GCCGGGAGCG TGGTGGCCGA CGTGGTGTTT 
GTGATTGAGG GTACGGCCAA CCTGGGACCC TACTTCGAGG GGCTCCGCAA GCACTACCTG 
CTCCCGGCCA TCGAGTATTT TAATGGTGGT CCTCCTGCTG AGACGGACTT CGGGGGAGAC 
TATGGGGGGA CCCAGTACAG CCTCGTGGTG TTCAACACAG TGGACTGCGC TCCCGAGTCC 
TACGTACAAT GTCACGCTCC CACCAGCAGC GCCTATGAGT TTGTCACCTG GCTCGATGGC 
ATTAAGTTCA TGGGCGGGGG TGGTGAGAGC TGCAGCCTCA TCGCGGAAGG ACTCAGCACA 
GCCTTGCAGC TGTTTGATGA CTTCAAGAAG ATGCGCGAGC AGATTGGCCA GACGCACCGG 
GTCTGCCTCC TCATCTGCAA CTCACCCCCA TACTTGTTGC CTGCTGTTGA GAGCACCACG 
TACTCTGGAT GCACAACTGA GAATCTTGTG CAGCAGATTG GGGAGCGGGG GATCCACTTC 
TCCATTGTGT CTCCCCGGAA GCTGCCTGCG CTTCGGCTTC TGTTTGAGAA GGCAGCCCCC 
CCGGCCTTGC TGGAGCCGCT GCAGCCTCCG ACAGATGTGA GCCAGGACCC GAGGCACATG 
GTGCTGGTTC GGGGACTCGT GCTGCCTGTT GGGGGTGGCT CAGCCCCAGG CCCCCTCCAG 
TCAAAGCAGC CAGTCCCCCT GCCTCCCGCC GCACCCTCAG GTGCCACTCT CTCAGCAGCC 
CCCCAGCAGC CTCTGCCCCC CGTCCCCCCG CAGTACCAGG TTCCCGGGAA CCTGAGTGCA 
GCTCAGGTGG CCGCGCAGAA TGCAGTGGAG GCTGCCAAGA ACCAGAAGGC TGGGCTGGGC 
CCTCGCTTCT CGCCCATCAC CCCTCTCCAA CAAGCTGCTC CCGGAGTGGG TCCCCCCTTC 
AGCCAGGCCC CAGCTCCCCA ACTACCCCCA GGACCCCCTG GCGCCCCCAA GCCACCACCT 
GCTTCCCAGC CCAGTCTGGT CTCCACTGTG GCCCCTGGCT CCGGCCTGGC TCCCACGGCA 
CAGCCCGGGG CACCGTCCAT GGCAGGCACT GTGGCCCCAG GAGGGGTGAG CGGCCCTTCC 
CCAGCCCAGC TGGGAGCCCC AGCCCTCGGT GGGCAGCAGT CAGTCTCCAA TAAGCTTCTG 
GCCTGGAGCG GGGTCCTGGA GTGGCAAGAG AAACCCAAAC CTGCCTCAGT GGATGCCAAC 
ACCAAGCTGA CGCGGTCACT GCCCTGCCAG GTCTACGTGA ATCATGGCGA GAACCTGAAG 
ACGGAGCAGT GGCCCCAGAA GCTGATCATG CAGCTCATCC CCCAGCAGCT GCTGACCACC 
CTGGGCCCTT TGTTCCGGAA CTCAAGGATG GTCCAGTTCC ATTTCACCAA CAAGGACCTG 
GAGTCTCTCA AAGGCCTCTA CCGCATCATG GGCAACGGCT TCGCGGGCTG CGTGCACTTC 
CCCCACACGG CGCCCTGTGA GGTGCGCGTG CTCATGCTCC TGTACTCGTC CAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAACGGCAT CCGGCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AGCAGCAGCG AGGAATGGGG 
GGACAGCAGG CACCCCCAGG GCTGGGGCCC ATTCTGGAGG ACCAAGCCAG GCCCTCACAG 
AATCTGCTCC AGCTCCGCCC ACCGCAGCCC CAGCCTCAGG GTACCGTAGG GGCCTCTGGG 
GCCACGGGGC AGCCCCAGCC CCAAGGTACT GCCCAGCCCC CGCCAGGTGC CCCTCAAGGC 
CCTCCTGGAG CAGCTTCTGG CCCACCCCCT CCTGGACCCA TCCTTCGGCC CCAGAACCCT 
GGGGCCAACC CTCAGCTGCG AAGCCTCCTC CTCAACCCAC CACCGCCGCA GACTGGGGTG 
CCCCCACCCC AGGCCTCCCT CCACCACCTC CAGCCACCAG GGGCTCCTGC GCTGCTGCCT 
CCGCCGCACC AGGGCCTGGG GCAGCCCCAG TTGGGGCCCC CACTCCTGCA TCCACCACCT 
GCCCAGTCCT GGCCCGCACA ACTTCCCCCT CGGGCTCCAC TGCCAGGTCA GATGCTGCTG 
AGCGGGGGTC CCCGGGGCCC GGTCCCCCAG CCGGGCCTGC AGCCCAGCGT CATGGAGGAC 
GACATCCTCA TGGATCTCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED25 ( XM_011527353.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525655.1 Homo sapiens mediator complex subunit 25 (MED25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu73001
Clone ID Related Accession (Same CDS sequence)	XM_011527353.1
Accession Version	XM_011527353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCCG GGTCCGAGGG CCCGGCCCGC GCCGGGAGCG TGGTGGCCGA CGTGGTGTTT 
GTGATTGAGG GTACGGCCAA CCTGGGACCC TACTTCGAGG GGCTCCGCAA GCACTACCTG 
CTCCCGGCCA TCGAGTATTT TAATGGTGGT CCTCCTGCTG AGACGGACTT CGGGGGAGAC 
TATGGGGGGA CCCAGTACAG CCTCGTGGTG TTCAACACAG TGGACTGCGC TCCCGAGTCC 
TACGTACAAT GTCACGCTCC CACCAGCAGC GCCTATGAGT TTGTCACCTG GCTCGATGGC 
ATTAAGTTCA TGGGCGGGGG TGGTGAGAGC TGCAGCCTCA TCGCGGAAGG ACTCAGCACA 
GCCTTGCAGC TGTTTGATGA CTTCAAGAAG ATGCGCGAGC AGATTGGCCA GACGCACCGG 
GTCTGCCTCC TCATCTGCAA CTCACCCCCA TACTTGTTGC CTGCTGTTGA GAGCACCACG 
TACTCTGGAT GCACAACTGA GAATCTTGTG CAGCAGATTG GGGAGCGGGG GATCCACTTC 
TCCATTGTGT CTCCCCGGAA GCTGCCTGCG CTTCGGCTTC TGTTTGAGAA GGCAGCCCCC 
CCGGCCTTGC TGGAGCCGCT GCAGCCTCCG ACAGATGTGA GCCAGGACCC GAGGCACATG 
GTGCTGGTTC GGGGACTCGT GCTGCCTGTT GGGGGTGGCT CAGCCCCAGG CCCCCTCCAG 
TCAAAGCAGC CAGTCCCCCT GCCTCCCGCC GCACCCTCAG GTGCCACTCT CTCAGCAGCC 
CCCCAGCAGC CTCTGCCCCC CGTCCCCCCG CAGTACCAGG TTCCCGGGAA CCTGAGTGCA 
GCTCAGGTGG CCGCGCAGAA TGCAGTGGAG GCTGCCAAGA ACCAGAAGGC TGGGCTGGGC 
CCTCGCTTCT CGCCCATCAC CCCTCTCCAA CAAGCTGCTC CCGGAGTGGG TCCCCCCTTC 
AGCCAGGCCC CAGCTCCCCA ACTACCCCCA GGACCCCCTG GCGCCCCCAA GCCACCACCT 
GCTTCCCAGC CCAGTCTGGT CTCCACTGTG GCCCCTGGCT CCGGCCTGGC TCCCACGGCA 
CAGCCCGGGG CACCGTCCAT GGCAGGCACT GTGGCCCCAG GAGGGGTGAG CGGCCCTTCC 
CCAGCCCAGC TGGGAGCCCC AGCCCTCGGT GGGCAGCAGT CAGTCTCCAA TAAGCTTCTG 
GCCTGGAGCG GGGTCCTGGA GTGGCAAGAG AAACCCAAAC CTGCCTCAGT GGATGCCAAC 
ACCAAGCTGA CGCGGTCACT GCCCTGCCAG GTCTACGTGA ATCATGGCGA GAACCTGAAG 
ACGGAGCAGT GGCCCCAGAA GCTGATCATG CAGCTCATCC CCCAGCAGCT GCTGACCACC 
CTGGGCCCTT TGTTCCGGAA CTCAAGGATG GTCCAGTTCC ATTTCACCAA CAAGGACCTG 
GAGTCTCTCA AAGGCCTCTA CCGCATCATG GGCAACGGCT TCGCGGGCTG CGTGCACTTC 
CCCCACACGG CGCCCTGTGA GGTGCGCGTG CTCATGCTCC TGTACTCGTC CAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAACGGCAT CCGGCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AGCAGCAGCG AGGAATGGGG 
GGACAGCAGG CACCCCCAGG GCTGGGGCCC ATTCTGGAGG ACCAAGCCAG GCCCTCACAG 
AATCTGCTCC AGCTCCGCCC ACCGCAGCCC CAGCCTCAGG GTACCGTAGG GGCCTCTGGG 
GCCACGGGGC AGCCCCAGCC CCAAGGTACT GCCCAGCCCC CGCCAGGTGC CCCTCAAGGC 
CCTCCTGGAG CAGCTTCTGG CCCACCCCCT CCTGGACCCA TCCTTCGGCC CCAGAACCCT 
GGGGCCAACC CTCAGCTGCG AAGCCTCCTC CTCAACCCAC CACCGCCGCA GACTGGGGTG 
CCCCCACCCC AGGCCTCCCT CCACCACCTC CAGCCACCAG GGGCTCCTGC GCTGCTGCCT 
CCGCCGCACC AGGGCCTGGG GCAGCCCCAG TTGGGGCCCC CACTCCTGCA TCCACCACCT 
GCCCAGTCCT GGCCCGCACA ACTTCCCCCT CGGGCTCCAC TGCCAGCGGC AAAACGAAAG 
AGAGAAGGAG AGGGCCGTGT GTTTCGAGAA AAATGGGAGC GAGCCTATTT CTTTGTGGAA 
GTGAAGAGCA TGCCTATGTG TTTAATATGC AAACAAATCG TGTCTGTGTT GAAAGAATAC 
AACCTGAAAC GCCACTATGA ATCAAAGCAT AGTAAGAGCT ACGACCAGTA CACAGAACAG 
ACTCGAGATG CCATCCTCAA CGAACTGAAA AAGGGACTCA AATGTCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525655.1
CDS	56..2446
Translation	MVPGSEGPARAGSVVADVVFVIEGTANLGPYFEGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTENLVQQIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQPPTDVSQDPRHMVLVRGLVLPVGGGSAPGPLQSKQPVPLPPAAPSGATLSAAPQQPLPPVPPQYQVPGNLSAAQVAAQNAVEAAKNQKAGLGPRFSPITPLQQAAPGVGPPFSQAPAPQLPPGPPGAPKPPPASQPSLVSTVAPGSGLAPTAQPGAPSMAGTVAPGGVSGPSPAQLGAPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQQRGMGGQQAPPGLGPILEDQARPSQNLLQLRPPQPQPQGTVGASGATGQPQPQGTAQPPPGAPQGPPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPGAPALLPPPHQGLGQPQLGPPLLHPPPAQSWPAQLPPRAPLPAAKRKREGEGRVFREKWERAYFFVEVKSMPMCLICKQIVSVLKEYNLKRHYESKHSKSYDQYTEQTRDAILNELKKGLKCQ

Target ORF information:

RefSeq Version	XM_011527353.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 25 (MED25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527353.1

ORF Insert Sequence:

ATGGTCCCCG GGTCCGAGGG CCCGGCCCGC GCCGGGAGCG TGGTGGCCGA CGTGGTGTTT 
GTGATTGAGG GTACGGCCAA CCTGGGACCC TACTTCGAGG GGCTCCGCAA GCACTACCTG 
CTCCCGGCCA TCGAGTATTT TAATGGTGGT CCTCCTGCTG AGACGGACTT CGGGGGAGAC 
TATGGGGGGA CCCAGTACAG CCTCGTGGTG TTCAACACAG TGGACTGCGC TCCCGAGTCC 
TACGTACAAT GTCACGCTCC CACCAGCAGC GCCTATGAGT TTGTCACCTG GCTCGATGGC 
ATTAAGTTCA TGGGCGGGGG TGGTGAGAGC TGCAGCCTCA TCGCGGAAGG ACTCAGCACA 
GCCTTGCAGC TGTTTGATGA CTTCAAGAAG ATGCGCGAGC AGATTGGCCA GACGCACCGG 
GTCTGCCTCC TCATCTGCAA CTCACCCCCA TACTTGTTGC CTGCTGTTGA GAGCACCACG 
TACTCTGGAT GCACAACTGA GAATCTTGTG CAGCAGATTG GGGAGCGGGG GATCCACTTC 
TCCATTGTGT CTCCCCGGAA GCTGCCTGCG CTTCGGCTTC TGTTTGAGAA GGCAGCCCCC 
CCGGCCTTGC TGGAGCCGCT GCAGCCTCCG ACAGATGTGA GCCAGGACCC GAGGCACATG 
GTGCTGGTTC GGGGACTCGT GCTGCCTGTT GGGGGTGGCT CAGCCCCAGG CCCCCTCCAG 
TCAAAGCAGC CAGTCCCCCT GCCTCCCGCC GCACCCTCAG GTGCCACTCT CTCAGCAGCC 
CCCCAGCAGC CTCTGCCCCC CGTCCCCCCG CAGTACCAGG TTCCCGGGAA CCTGAGTGCA 
GCTCAGGTGG CCGCGCAGAA TGCAGTGGAG GCTGCCAAGA ACCAGAAGGC TGGGCTGGGC 
CCTCGCTTCT CGCCCATCAC CCCTCTCCAA CAAGCTGCTC CCGGAGTGGG TCCCCCCTTC 
AGCCAGGCCC CAGCTCCCCA ACTACCCCCA GGACCCCCTG GCGCCCCCAA GCCACCACCT 
GCTTCCCAGC CCAGTCTGGT CTCCACTGTG GCCCCTGGCT CCGGCCTGGC TCCCACGGCA 
CAGCCCGGGG CACCGTCCAT GGCAGGCACT GTGGCCCCAG GAGGGGTGAG CGGCCCTTCC 
CCAGCCCAGC TGGGAGCCCC AGCCCTCGGT GGGCAGCAGT CAGTCTCCAA TAAGCTTCTG 
GCCTGGAGCG GGGTCCTGGA GTGGCAAGAG AAACCCAAAC CTGCCTCAGT GGATGCCAAC 
ACCAAGCTGA CGCGGTCACT GCCCTGCCAG GTCTACGTGA ATCATGGCGA GAACCTGAAG 
ACGGAGCAGT GGCCCCAGAA GCTGATCATG CAGCTCATCC CCCAGCAGCT GCTGACCACC 
CTGGGCCCTT TGTTCCGGAA CTCAAGGATG GTCCAGTTCC ATTTCACCAA CAAGGACCTG 
GAGTCTCTCA AAGGCCTCTA CCGCATCATG GGCAACGGCT TCGCGGGCTG CGTGCACTTC 
CCCCACACGG CGCCCTGTGA GGTGCGCGTG CTCATGCTCC TGTACTCGTC CAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAACGGCAT CCGGCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AGCAGCAGCG AGGAATGGGG 
GGACAGCAGG CACCCCCAGG GCTGGGGCCC ATTCTGGAGG ACCAAGCCAG GCCCTCACAG 
AATCTGCTCC AGCTCCGCCC ACCGCAGCCC CAGCCTCAGG GTACCGTAGG GGCCTCTGGG 
GCCACGGGGC AGCCCCAGCC CCAAGGTACT GCCCAGCCCC CGCCAGGTGC CCCTCAAGGC 
CCTCCTGGAG CAGCTTCTGG CCCACCCCCT CCTGGACCCA TCCTTCGGCC CCAGAACCCT 
GGGGCCAACC CTCAGCTGCG AAGCCTCCTC CTCAACCCAC CACCGCCGCA GACTGGGGTG 
CCCCCACCCC AGGCCTCCCT CCACCACCTC CAGCCACCAG GGGCTCCTGC GCTGCTGCCT 
CCGCCGCACC AGGGCCTGGG GCAGCCCCAG TTGGGGCCCC CACTCCTGCA TCCACCACCT 
GCCCAGTCCT GGCCCGCACA ACTTCCCCCT CGGGCTCCAC TGCCAGCGGC AAAACGAAAG 
AGAGAAGGAG AGGGCCGTGT GTTTCGAGAA AAATGGGAGC GAGCCTATTT CTTTGTGGAA 
GTGAAGAGCA TGCCTATGTG TTTAATATGC AAACAAATCG TGTCTGTGTT GAAAGAATAC 
AACCTGAAAC GCCACTATGA ATCAAAGCAT AGTAAGAGCT ACGACCAGTA CACAGAACAG 
ACTCGAGATG CCATCCTCAA CGAACTGAAA AAGGGACTCA AATGTCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ETV4 and AP1 Transcription Factors Form Multivalent Interactions with three Sites on the MED25 Activator-Interacting Domain.
Journal of molecular biology429(20)2975-2995(2017 Oct)
Currie SL,Doane JJ,Evans KS,Bhachech N,Madison BJ,Lau DKW,McIntosh LP,Skalicky JJ,Clark KA,Graves BJ

Upregulation of human CYP2C9 expression by Bisphenol A via estrogen receptor alpha (ER?) and Med25.
Environmental toxicology32(3)970-978(2017 Mar)
Xu JY,Wu L,Shi Z,Zhang XJ,Englert NA,Zhang SY

Frameshift Mutation of MED25, a Transcription Regulator, and its Mutational Heterogeneity in Colorectal Cancers.
Pathology oncology research : POR22(4)875-6(2016 Oct)
Jo YS,Kim MS,Yoo NJ,Lee SH

Characterization of ERM transactivation domain binding to the ACID/PTOV domain of the Mediator subunit MED25.
Nucleic acids research43(14)7110-21(2015 Aug)
Landrieu I,Verger A,Baert JL,Rucktooa P,Cantrelle FX,Dewitte F,Ferreira E,Lens Z,Villeret V,Mont? D

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MED25 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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