MED25 cDNA ORF clone, Canis lupus familiaris(dog)

The following MED25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe34927 XM_005616290.3
Latest version!
Canis lupus familiaris mediator complex subunit 25 (MED25), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe34928 XM_541489.7
Latest version!
Canis lupus familiaris mediator complex subunit 25 (MED25), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OCe34928 XM_541489.6 Canis lupus familiaris mediator complex subunit 25 (MED25), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe34927 XM_005616290.2 Canis lupus familiaris mediator complex subunit 25 (MED25), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe34927
    Clone ID Related Accession (Same CDS sequence) XM_005616290.2 , XM_005616290.3
    Accession Version XM_005616290.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005616290.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTCCCCG GGTCGGAGGG CCCGGCCCGC GCCGGGGGCC TCGTGGCTGA CGTGGTGTTT 
    GTGATCGAGG GTACAGCCAA CCTGGGGCCT TACTTCGAAG GGTTGCGCAA GCACTACCTG
    CTTCCGGCCA TCGAGTATTT TAATGGTGGT CCTCCTGCCG AGACGGACTT CGGAGGAGAC
    TATGGGGGTA CCCAGTACAG CCTCGTGGTG TTCAACACGG TGGACTGCGC TCCCGAGTCC
    TACGTACAGT GTCACGCTCC CACCAGCAGC GCCTATGAGT TTGTCACCTG GCTCGATGGC
    ATTAAGTTCA TGGGCGGGGG TGGTGAGAGT TGTAGCCTCA TCGCGGAAGG CCTCAGCACG
    GCCCTGCAGC TCTTCGATGA CTTCAAGAAG ATGCGGGAGC AGATCGGCCA GACACACCGC
    GTCTGCCTCC TCATCTGTAA CTCACCCCCG TACCTGCTCC CTGCCGTCGA GAGTACCACA
    TACTCTGGGT ACACCACAGA GAGCCTCGTG CAGAAGATAG GTGAGCAAGG GATCCACTTC
    TCCATCGTGT CTCCCCGGAA GCTGCCTGCC CTGAGGCTTC TGTTCGAGAA GGCAGCTCCA
    CCGGCCTTGC TGGAGCCGCT GCAGCCACCA ACAGATGTGA GCCAGGATCC CCGGCACATG
    GTGCTGGTGC GGGGGCTTGT GCTGCCAGTT GGGGGTGGCT CAGCCCCAGG CCCCCTGCAG
    CCAAAGCAGC CGGTCCCCCT GCCTCCAGCT CCACCCTCGG GTGCCTCACT CTCAGCAGCG
    CCCCAGCAGC CTCTGCCCCC CGTCCCTCAG CAGTACCAGG TCCCTGGGAA CCTGAGTGCA
    GCTCAGGTGG CTGCCCAGAA TGCAGTGGAA GCCGCCAAGA ACCAGAAGGC TGGGCTGGGC
    CCCCGCTTCT CACCCATTAA CCCTCTCCAA CAAGCTGCTC CAGGAGTGGG TCCCCCCTTC
    AGCCAGGCAC CGGCCCCACC ACTACCCCCG GGGCCCCCTG GCGCCCCCAA GCCACCCCCT
    GCTTCCCAGC CCAGCCTGGT CTCCACTGTG GCTCCTGGCC CAGGCCTGGC CCCCCCTGCA
    CAACCTGGGG CCCCATCCAT GGCGGGCACG GTGGCCCCAG GTGGGGTGAG TGGCCCTGCC
    CCGGCGCAGC TGGGGGCCCC GGCCCTTGGG GGGCAGCAGT CAGTGTCCAA CAAACTCCTG
    GCCTGGAGCG GGGTCCTCGA ATGGCAGGAG AAGCCCAAAC CTGCCTCCGT GGACGCCAAC
    ACCAAGCTGA CGCGATCGCT GCCTTGCCAG GTCTATGTGA ATCACGGCGA GAACTTAAAG
    ACTGAGCAGT GGCCTCAGAA GCTGATCATG CAGCTCATCC CACAGCAGTT GCTGACCACC
    CTGGGCCCTT TGTTCCGGAA CTCAAGGATG GTGCAGTTCC ATTTCACCAA CAAGGACCTG
    GAGTCCCTGA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCTGGCTG TGTGCACTTC
    CCCCACACGG CCCCCTGTGA GGTGCGTGTG CTCATGCTCC TGTACTCGTC CAAGAAGAAG
    ATTTTCATGG GCCTCATCCC CTATGACCAG AGCGGCTTCG TCAATGGCAT CCGGCAGGTC
    ATCACCAACC ACAAGCAAGT CCAGCAGCAG AAGCTGGAGC AGCAGCGTGT GATGGGGGCA
    CAGCAGGCAC CCCCTGGGCT GGGCCCCATT CTGGAGGACC AGGCGAGACC CTCACAGAAT
    CTGCTCCAGC TCCGCCCACC ACAGCCCCAA CCTCAGGGCA CTGTGGGGGC CTCTGCGGCC
    TCAGGACAGC CCCAGCCCCA AGGGGCTGCC CAGGCCCCCC CAGGTGCCCC CCAAGGTCCT
    CCTGGAGCAG CCCCTGGCCC CCCCCCTCCT GGACCCATCC TTCGGCCTCA GAACCCTGGG
    GCCAACCCCC AACTGCGGAG TCTCCTCCTC AACCCACCTC CGCCTCAGAC TGGGGTGCCC
    CCACCCCAAG CTTCCCTCCA CCACCTCCAG CCACCAGGGG CTCCAGCACT GTTGCCACCA
    CACCAGGGCC TGGGGCAACC CCAGCTGGGG CCCCCCCTCC TGCACCCACC ACCTGCCCAG
    TCCTGGCCCA CACAGCTTCC CCCGAGAGCT CCGCTGCCAG TGGCAAAACG AAAGAGAGAC
    CGAGAGGGCC ACGTGTTTCG AGAAAAATGG GAGCGAGCCT ATTTCTTTGT GGAAGTGAAG
    AGCATGCCTA TGTGTTTAAT ATGCAAAAAA ATCGTATCTG TGTTGAAAGA ATACAACCTG
    AGACGTCATT ACGAATCAAA GCACAGTAAG AGCTTCGACC AGTATACAGA ACAGATGCGA
    GATGCGATAC TCGCTGAGCT GAAAAAGGGC CTCAAGTGTC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616347.2
    CDS78..2462
    Translation

    Target ORF information:

    RefSeq Version XM_005616290.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mediator complex subunit 25 (MED25), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616290.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTCCCCG GGTCGGAGGG CCCGGCCCGC GCCGGGGGCC TCGTGGCTGA CGTGGTGTTT 
    GTGATCGAGG GTACAGCCAA CCTGGGGCCT TACTTCGAAG GGTTGCGCAA GCACTACCTG
    CTTCCGGCCA TCGAGTATTT TAATGGTGGT CCTCCTGCCG AGACGGACTT CGGAGGAGAC
    TATGGGGGTA CCCAGTACAG CCTCGTGGTG TTCAACACGG TGGACTGCGC TCCCGAGTCC
    TACGTACAGT GTCACGCTCC CACCAGCAGC GCCTATGAGT TTGTCACCTG GCTCGATGGC
    ATTAAGTTCA TGGGCGGGGG TGGTGAGAGT TGTAGCCTCA TCGCGGAAGG CCTCAGCACG
    GCCCTGCAGC TCTTCGATGA CTTCAAGAAG ATGCGGGAGC AGATCGGCCA GACACACCGC
    GTCTGCCTCC TCATCTGTAA CTCACCCCCG TACCTGCTCC CTGCCGTCGA GAGTACCACA
    TACTCTGGGT ACACCACAGA GAGCCTCGTG CAGAAGATAG GTGAGCAAGG GATCCACTTC
    TCCATCGTGT CTCCCCGGAA GCTGCCTGCC CTGAGGCTTC TGTTCGAGAA GGCAGCTCCA
    CCGGCCTTGC TGGAGCCGCT GCAGCCACCA ACAGATGTGA GCCAGGATCC CCGGCACATG
    GTGCTGGTGC GGGGGCTTGT GCTGCCAGTT GGGGGTGGCT CAGCCCCAGG CCCCCTGCAG
    CCAAAGCAGC CGGTCCCCCT GCCTCCAGCT CCACCCTCGG GTGCCTCACT CTCAGCAGCG
    CCCCAGCAGC CTCTGCCCCC CGTCCCTCAG CAGTACCAGG TCCCTGGGAA CCTGAGTGCA
    GCTCAGGTGG CTGCCCAGAA TGCAGTGGAA GCCGCCAAGA ACCAGAAGGC TGGGCTGGGC
    CCCCGCTTCT CACCCATTAA CCCTCTCCAA CAAGCTGCTC CAGGAGTGGG TCCCCCCTTC
    AGCCAGGCAC CGGCCCCACC ACTACCCCCG GGGCCCCCTG GCGCCCCCAA GCCACCCCCT
    GCTTCCCAGC CCAGCCTGGT CTCCACTGTG GCTCCTGGCC CAGGCCTGGC CCCCCCTGCA
    CAACCTGGGG CCCCATCCAT GGCGGGCACG GTGGCCCCAG GTGGGGTGAG TGGCCCTGCC
    CCGGCGCAGC TGGGGGCCCC GGCCCTTGGG GGGCAGCAGT CAGTGTCCAA CAAACTCCTG
    GCCTGGAGCG GGGTCCTCGA ATGGCAGGAG AAGCCCAAAC CTGCCTCCGT GGACGCCAAC
    ACCAAGCTGA CGCGATCGCT GCCTTGCCAG GTCTATGTGA ATCACGGCGA GAACTTAAAG
    ACTGAGCAGT GGCCTCAGAA GCTGATCATG CAGCTCATCC CACAGCAGTT GCTGACCACC
    CTGGGCCCTT TGTTCCGGAA CTCAAGGATG GTGCAGTTCC ATTTCACCAA CAAGGACCTG
    GAGTCCCTGA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCTGGCTG TGTGCACTTC
    CCCCACACGG CCCCCTGTGA GGTGCGTGTG CTCATGCTCC TGTACTCGTC CAAGAAGAAG
    ATTTTCATGG GCCTCATCCC CTATGACCAG AGCGGCTTCG TCAATGGCAT CCGGCAGGTC
    ATCACCAACC ACAAGCAAGT CCAGCAGCAG AAGCTGGAGC AGCAGCGTGT GATGGGGGCA
    CAGCAGGCAC CCCCTGGGCT GGGCCCCATT CTGGAGGACC AGGCGAGACC CTCACAGAAT
    CTGCTCCAGC TCCGCCCACC ACAGCCCCAA CCTCAGGGCA CTGTGGGGGC CTCTGCGGCC
    TCAGGACAGC CCCAGCCCCA AGGGGCTGCC CAGGCCCCCC CAGGTGCCCC CCAAGGTCCT
    CCTGGAGCAG CCCCTGGCCC CCCCCCTCCT GGACCCATCC TTCGGCCTCA GAACCCTGGG
    GCCAACCCCC AACTGCGGAG TCTCCTCCTC AACCCACCTC CGCCTCAGAC TGGGGTGCCC
    CCACCCCAAG CTTCCCTCCA CCACCTCCAG CCACCAGGGG CTCCAGCACT GTTGCCACCA
    CACCAGGGCC TGGGGCAACC CCAGCTGGGG CCCCCCCTCC TGCACCCACC ACCTGCCCAG
    TCCTGGCCCA CACAGCTTCC CCCGAGAGCT CCGCTGCCAG TGGCAAAACG AAAGAGAGAC
    CGAGAGGGCC ACGTGTTTCG AGAAAAATGG GAGCGAGCCT ATTTCTTTGT GGAAGTGAAG
    AGCATGCCTA TGTGTTTAAT ATGCAAAAAA ATCGTATCTG TGTTGAAAGA ATACAACCTG
    AGACGTCATT ACGAATCAAA GCACAGTAAG AGCTTCGACC AGTATACAGA ACAGATGCGA
    GATGCGATAC TCGCTGAGCT GAAAAAGGGC CTCAAGTGTC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34927
    Clone ID Related Accession (Same CDS sequence) XM_005616290.2 , XM_005616290.3
    Accession Version XM_005616290.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005616290.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTCCCCG GGTCGGAGGG CCCGGCCCGC GCCGGGGGCC TCGTGGCTGA CGTGGTGTTT 
    GTGATCGAGG GTACAGCCAA CCTGGGGCCT TACTTCGAAG GGTTGCGCAA GCACTACCTG
    CTTCCGGCCA TCGAGTATTT TAATGGTGGT CCTCCTGCCG AGACGGACTT CGGAGGAGAC
    TATGGGGGTA CCCAGTACAG CCTCGTGGTG TTCAACACGG TGGACTGCGC TCCCGAGTCC
    TACGTACAGT GTCACGCTCC CACCAGCAGC GCCTATGAGT TTGTCACCTG GCTCGATGGC
    ATTAAGTTCA TGGGCGGGGG TGGTGAGAGT TGTAGCCTCA TCGCGGAAGG CCTCAGCACG
    GCCCTGCAGC TCTTCGATGA CTTCAAGAAG ATGCGGGAGC AGATCGGCCA GACACACCGC
    GTCTGCCTCC TCATCTGTAA CTCACCCCCG TACCTGCTCC CTGCCGTCGA GAGTACCACA
    TACTCTGGGT ACACCACAGA GAGCCTCGTG CAGAAGATAG GTGAGCAAGG GATCCACTTC
    TCCATCGTGT CTCCCCGGAA GCTGCCTGCC CTGAGGCTTC TGTTCGAGAA GGCAGCTCCA
    CCGGCCTTGC TGGAGCCGCT GCAGCCACCA ACAGATGTGA GCCAGGATCC CCGGCACATG
    GTGCTGGTGC GGGGGCTTGT GCTGCCAGTT GGGGGTGGCT CAGCCCCAGG CCCCCTGCAG
    CCAAAGCAGC CGGTCCCCCT GCCTCCAGCT CCACCCTCGG GTGCCTCACT CTCAGCAGCG
    CCCCAGCAGC CTCTGCCCCC CGTCCCTCAG CAGTACCAGG TCCCTGGGAA CCTGAGTGCA
    GCTCAGGTGG CTGCCCAGAA TGCAGTGGAA GCCGCCAAGA ACCAGAAGGC TGGGCTGGGC
    CCCCGCTTCT CACCCATTAA CCCTCTCCAA CAAGCTGCTC CAGGAGTGGG TCCCCCCTTC
    AGCCAGGCAC CGGCCCCACC ACTACCCCCG GGGCCCCCTG GCGCCCCCAA GCCACCCCCT
    GCTTCCCAGC CCAGCCTGGT CTCCACTGTG GCTCCTGGCC CAGGCCTGGC CCCCCCTGCA
    CAACCTGGGG CCCCATCCAT GGCGGGCACG GTGGCCCCAG GTGGGGTGAG TGGCCCTGCC
    CCGGCGCAGC TGGGGGCCCC GGCCCTTGGG GGGCAGCAGT CAGTGTCCAA CAAACTCCTG
    GCCTGGAGCG GGGTCCTCGA ATGGCAGGAG AAGCCCAAAC CTGCCTCCGT GGACGCCAAC
    ACCAAGCTGA CGCGATCGCT GCCTTGCCAG GTCTATGTGA ATCACGGCGA GAACTTAAAG
    ACTGAGCAGT GGCCTCAGAA GCTGATCATG CAGCTCATCC CACAGCAGTT GCTGACCACC
    CTGGGCCCTT TGTTCCGGAA CTCAAGGATG GTGCAGTTCC ATTTCACCAA CAAGGACCTG
    GAGTCCCTGA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCTGGCTG TGTGCACTTC
    CCCCACACGG CCCCCTGTGA GGTGCGTGTG CTCATGCTCC TGTACTCGTC CAAGAAGAAG
    ATTTTCATGG GCCTCATCCC CTATGACCAG AGCGGCTTCG TCAATGGCAT CCGGCAGGTC
    ATCACCAACC ACAAGCAAGT CCAGCAGCAG AAGCTGGAGC AGCAGCGTGT GATGGGGGCA
    CAGCAGGCAC CCCCTGGGCT GGGCCCCATT CTGGAGGACC AGGCGAGACC CTCACAGAAT
    CTGCTCCAGC TCCGCCCACC ACAGCCCCAA CCTCAGGGCA CTGTGGGGGC CTCTGCGGCC
    TCAGGACAGC CCCAGCCCCA AGGGGCTGCC CAGGCCCCCC CAGGTGCCCC CCAAGGTCCT
    CCTGGAGCAG CCCCTGGCCC CCCCCCTCCT GGACCCATCC TTCGGCCTCA GAACCCTGGG
    GCCAACCCCC AACTGCGGAG TCTCCTCCTC AACCCACCTC CGCCTCAGAC TGGGGTGCCC
    CCACCCCAAG CTTCCCTCCA CCACCTCCAG CCACCAGGGG CTCCAGCACT GTTGCCACCA
    CACCAGGGCC TGGGGCAACC CCAGCTGGGG CCCCCCCTCC TGCACCCACC ACCTGCCCAG
    TCCTGGCCCA CACAGCTTCC CCCGAGAGCT CCGCTGCCAG TGGCAAAACG AAAGAGAGAC
    CGAGAGGGCC ACGTGTTTCG AGAAAAATGG GAGCGAGCCT ATTTCTTTGT GGAAGTGAAG
    AGCATGCCTA TGTGTTTAAT ATGCAAAAAA ATCGTATCTG TGTTGAAAGA ATACAACCTG
    AGACGTCATT ACGAATCAAA GCACAGTAAG AGCTTCGACC AGTATACAGA ACAGATGCGA
    GATGCGATAC TCGCTGAGCT GAAAAAGGGC CTCAAGTGTC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616347.2
    CDS46..2430
    Translation

    Target ORF information:

    RefSeq Version XM_005616290.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mediator complex subunit 25 (MED25), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616290.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTCCCCG GGTCGGAGGG CCCGGCCCGC GCCGGGGGCC TCGTGGCTGA CGTGGTGTTT 
    GTGATCGAGG GTACAGCCAA CCTGGGGCCT TACTTCGAAG GGTTGCGCAA GCACTACCTG
    CTTCCGGCCA TCGAGTATTT TAATGGTGGT CCTCCTGCCG AGACGGACTT CGGAGGAGAC
    TATGGGGGTA CCCAGTACAG CCTCGTGGTG TTCAACACGG TGGACTGCGC TCCCGAGTCC
    TACGTACAGT GTCACGCTCC CACCAGCAGC GCCTATGAGT TTGTCACCTG GCTCGATGGC
    ATTAAGTTCA TGGGCGGGGG TGGTGAGAGT TGTAGCCTCA TCGCGGAAGG CCTCAGCACG
    GCCCTGCAGC TCTTCGATGA CTTCAAGAAG ATGCGGGAGC AGATCGGCCA GACACACCGC
    GTCTGCCTCC TCATCTGTAA CTCACCCCCG TACCTGCTCC CTGCCGTCGA GAGTACCACA
    TACTCTGGGT ACACCACAGA GAGCCTCGTG CAGAAGATAG GTGAGCAAGG GATCCACTTC
    TCCATCGTGT CTCCCCGGAA GCTGCCTGCC CTGAGGCTTC TGTTCGAGAA GGCAGCTCCA
    CCGGCCTTGC TGGAGCCGCT GCAGCCACCA ACAGATGTGA GCCAGGATCC CCGGCACATG
    GTGCTGGTGC GGGGGCTTGT GCTGCCAGTT GGGGGTGGCT CAGCCCCAGG CCCCCTGCAG
    CCAAAGCAGC CGGTCCCCCT GCCTCCAGCT CCACCCTCGG GTGCCTCACT CTCAGCAGCG
    CCCCAGCAGC CTCTGCCCCC CGTCCCTCAG CAGTACCAGG TCCCTGGGAA CCTGAGTGCA
    GCTCAGGTGG CTGCCCAGAA TGCAGTGGAA GCCGCCAAGA ACCAGAAGGC TGGGCTGGGC
    CCCCGCTTCT CACCCATTAA CCCTCTCCAA CAAGCTGCTC CAGGAGTGGG TCCCCCCTTC
    AGCCAGGCAC CGGCCCCACC ACTACCCCCG GGGCCCCCTG GCGCCCCCAA GCCACCCCCT
    GCTTCCCAGC CCAGCCTGGT CTCCACTGTG GCTCCTGGCC CAGGCCTGGC CCCCCCTGCA
    CAACCTGGGG CCCCATCCAT GGCGGGCACG GTGGCCCCAG GTGGGGTGAG TGGCCCTGCC
    CCGGCGCAGC TGGGGGCCCC GGCCCTTGGG GGGCAGCAGT CAGTGTCCAA CAAACTCCTG
    GCCTGGAGCG GGGTCCTCGA ATGGCAGGAG AAGCCCAAAC CTGCCTCCGT GGACGCCAAC
    ACCAAGCTGA CGCGATCGCT GCCTTGCCAG GTCTATGTGA ATCACGGCGA GAACTTAAAG
    ACTGAGCAGT GGCCTCAGAA GCTGATCATG CAGCTCATCC CACAGCAGTT GCTGACCACC
    CTGGGCCCTT TGTTCCGGAA CTCAAGGATG GTGCAGTTCC ATTTCACCAA CAAGGACCTG
    GAGTCCCTGA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCTGGCTG TGTGCACTTC
    CCCCACACGG CCCCCTGTGA GGTGCGTGTG CTCATGCTCC TGTACTCGTC CAAGAAGAAG
    ATTTTCATGG GCCTCATCCC CTATGACCAG AGCGGCTTCG TCAATGGCAT CCGGCAGGTC
    ATCACCAACC ACAAGCAAGT CCAGCAGCAG AAGCTGGAGC AGCAGCGTGT GATGGGGGCA
    CAGCAGGCAC CCCCTGGGCT GGGCCCCATT CTGGAGGACC AGGCGAGACC CTCACAGAAT
    CTGCTCCAGC TCCGCCCACC ACAGCCCCAA CCTCAGGGCA CTGTGGGGGC CTCTGCGGCC
    TCAGGACAGC CCCAGCCCCA AGGGGCTGCC CAGGCCCCCC CAGGTGCCCC CCAAGGTCCT
    CCTGGAGCAG CCCCTGGCCC CCCCCCTCCT GGACCCATCC TTCGGCCTCA GAACCCTGGG
    GCCAACCCCC AACTGCGGAG TCTCCTCCTC AACCCACCTC CGCCTCAGAC TGGGGTGCCC
    CCACCCCAAG CTTCCCTCCA CCACCTCCAG CCACCAGGGG CTCCAGCACT GTTGCCACCA
    CACCAGGGCC TGGGGCAACC CCAGCTGGGG CCCCCCCTCC TGCACCCACC ACCTGCCCAG
    TCCTGGCCCA CACAGCTTCC CCCGAGAGCT CCGCTGCCAG TGGCAAAACG AAAGAGAGAC
    CGAGAGGGCC ACGTGTTTCG AGAAAAATGG GAGCGAGCCT ATTTCTTTGT GGAAGTGAAG
    AGCATGCCTA TGTGTTTAAT ATGCAAAAAA ATCGTATCTG TGTTGAAAGA ATACAACCTG
    AGACGTCATT ACGAATCAAA GCACAGTAAG AGCTTCGACC AGTATACAGA ACAGATGCGA
    GATGCGATAC TCGCTGAGCT GAAAAAGGGC CTCAAGTGTC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34928
    Clone ID Related Accession (Same CDS sequence) XM_541489.7 , XM_541489.6
    Accession Version XM_541489.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_541489.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGTCCCCG GGTCGGAGGG CCCGGCCCGC GCCGGGGGCC TCGTGGCTGA CGTGGTGTTT 
    GTGATCGAGG GTACAGCCAA CCTGGGGCCT TACTTCGAAG GGTTGCGCAA GCACTACCTG
    CTTCCGGCCA TCGAGTATTT TAATGGTGGT CCTCCTGCCG AGACGGACTT CGGAGGAGAC
    TATGGGGGTA CCCAGTACAG CCTCGTGGTG TTCAACACGG TGGACTGCGC TCCCGAGTCC
    TACGTACAGT GTCACGCTCC CACCAGCAGC GCCTATGAGT TTGTCACCTG GCTCGATGGC
    ATTAAGTTCA TGGGCGGGGG TGGTGAGAGT TGTAGCCTCA TCGCGGAAGG CCTCAGCACG
    GCCCTGCAGC TCTTCGATGA CTTCAAGAAG ATGCGGGAGC AGATCGGCCA GACACACCGC
    GTCTGCCTCC TCATCTGTAA CTCACCCCCG TACCTGCTCC CTGCCGTCGA GAGTACCACA
    TACTCTGGGT ACACCACAGA GAGCCTCGTG CAGAAGATAG GTGAGCAAGG GATCCACTTC
    TCCATCGTGT CTCCCCGGAA GCTGCCTGCC CTGAGGCTTC TGTTCGAGAA GGCAGCTCCA
    CCGGCCTTGC TGGAGCCGCT GCAGCCACCA ACAGATGTGA GCCAGGATCC CCGGCACATG
    GTGCTGGTGC GGGGGCTTGT GCTGCCAGTT GGGGGTGGCT CAGCCCCAGG CCCCCTGCAG
    CCAAAGCAGC CGGTCCCCCT GCCTCCAGCT CCACCCTCGG GTGCCTCACT CTCAGCAGCG
    CCCCAGCAGC CTCTGCCCCC CGTCCCTCAG CAGTACCAGG TCCCTGGGAA CCTGAGTGCA
    GCTCAGGTGG CTGCCCAGAA TGCAGTGGAA GCCGCCAAGA ACCAGAAGGC TGGGCTGGGC
    CCCCGCTTCT CACCCATTAA CCCTCTCCAA CAAGCTGCTC CAGGAGTGGG TCCCCCCTTC
    AGCCAGGCAC CGGCCCCACC ACTACCCCCG GGGCCCCCTG GCGCCCCCAA GCCACCCCCT
    GCTTCCCAGC CCAGCCTGGT CTCCACTGTG GCTCCTGGCC CAGGCCTGGC CCCCCCTGCA
    CAACCTGGGG CCCCATCCAT GGCGGGCACG GTGGCCCCAG GTGGGGTGAG TGGCCCTGCC
    CCGGCGCAGC TGGGGGCCCC GGCCCTTGGG GGGCAGCAGT CAGTGTCCAA CAAACTCCTG
    GCCTGGAGCG GGGTCCTCGA ATGGCAGGAG AAGCCCAAAC CTGCCTCCGT GGACGCCAAC
    ACCAAGCTGA CGCGATCGCT GCCTTGCCAG GTCTATGTGA ATCACGGCGA GAACTTAAAG
    ACTGAGCAGT GGCCTCAGAA GCTGATCATG CAGCTCATCC CACAGCAGTT GCTGACCACC
    CTGGGCCCTT TGTTCCGGAA CTCAAGGATG GTGCAGTTCC ATTTCACCAA CAAGGACCTG
    GAGTCCCTGA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCTGGCTG TGTGCACTTC
    CCCCACACGG CCCCCTGTGA GGTGCGTGTG CTCATGCTCC TGTACTCGTC CAAGAAGAAG
    ATTTTCATGG GCCTCATCCC CTATGACCAG AGCGGCTTCG TCAATGGCAT CCGGCAGGTC
    ATCACCAACC ACAAGCAAGT CCAGCAGCAG AAGCTGGAGC AGCAGCGTGT GATGGGGGCA
    CAGCAGGCAC CCCCTGGGCT GGGCCCCATT CTGGAGGACC AGGCGAGACC CTCACAGAAT
    CTGCTCCAGC TCCGCCCACC ACAGCCCCAA CCTCAGGGCA CTGTGGGGGC CTCTGCGGCC
    TCAGGACAGC CCCAGCCCCA AGGGGCTGCC CAGGCCCCCC CAGGTGCCCC CCAAGGTCCT
    CCTGGAGCAG CCCCTGGCCC CCCCCCTCCT GGACCCATCC TTCGGCCTCA GAACCCTGGG
    GCCAACCCCC AACTGCGGAG TCTCCTCCTC AACCCACCTC CGCCTCAGAC TGGGGTGCCC
    CCACCCCAAG CTTCCCTCCA CCACCTCCAG CCACCAGGGG CTCCAGCACT GTTGCCACCA
    CACCAGGGCC TGGGGCAACC CCAGCTGGGG CCCCCCCTCC TGCACCCACC ACCTGCCCAG
    TCCTGGCCCA CACAGCTTCC CCCGAGAGCT CCGCTGCCAG GTCAGATGCT GCTGAGCGGG
    GGTCCCCGGG GCCCGGTTCC CCAGCCGGGC CTGCAGCCCA GCGTCATGGA GGACGACATC
    CTCATGGATC TCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_541489.5
    CDS46..2283
    Translation

    Target ORF information:

    RefSeq Version XM_541489.7
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mediator complex subunit 25 (MED25), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_541489.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGTCCCCG GGTCGGAGGG CCCGGCCCGC GCCGGGGGCC TCGTGGCTGA CGTGGTGTTT 
    GTGATCGAGG GTACAGCCAA CCTGGGGCCT TACTTCGAAG GGTTGCGCAA GCACTACCTG
    CTTCCGGCCA TCGAGTATTT TAATGGTGGT CCTCCTGCCG AGACGGACTT CGGAGGAGAC
    TATGGGGGTA CCCAGTACAG CCTCGTGGTG TTCAACACGG TGGACTGCGC TCCCGAGTCC
    TACGTACAGT GTCACGCTCC CACCAGCAGC GCCTATGAGT TTGTCACCTG GCTCGATGGC
    ATTAAGTTCA TGGGCGGGGG TGGTGAGAGT TGTAGCCTCA TCGCGGAAGG CCTCAGCACG
    GCCCTGCAGC TCTTCGATGA CTTCAAGAAG ATGCGGGAGC AGATCGGCCA GACACACCGC
    GTCTGCCTCC TCATCTGTAA CTCACCCCCG TACCTGCTCC CTGCCGTCGA GAGTACCACA
    TACTCTGGGT ACACCACAGA GAGCCTCGTG CAGAAGATAG GTGAGCAAGG GATCCACTTC
    TCCATCGTGT CTCCCCGGAA GCTGCCTGCC CTGAGGCTTC TGTTCGAGAA GGCAGCTCCA
    CCGGCCTTGC TGGAGCCGCT GCAGCCACCA ACAGATGTGA GCCAGGATCC CCGGCACATG
    GTGCTGGTGC GGGGGCTTGT GCTGCCAGTT GGGGGTGGCT CAGCCCCAGG CCCCCTGCAG
    CCAAAGCAGC CGGTCCCCCT GCCTCCAGCT CCACCCTCGG GTGCCTCACT CTCAGCAGCG
    CCCCAGCAGC CTCTGCCCCC CGTCCCTCAG CAGTACCAGG TCCCTGGGAA CCTGAGTGCA
    GCTCAGGTGG CTGCCCAGAA TGCAGTGGAA GCCGCCAAGA ACCAGAAGGC TGGGCTGGGC
    CCCCGCTTCT CACCCATTAA CCCTCTCCAA CAAGCTGCTC CAGGAGTGGG TCCCCCCTTC
    AGCCAGGCAC CGGCCCCACC ACTACCCCCG GGGCCCCCTG GCGCCCCCAA GCCACCCCCT
    GCTTCCCAGC CCAGCCTGGT CTCCACTGTG GCTCCTGGCC CAGGCCTGGC CCCCCCTGCA
    CAACCTGGGG CCCCATCCAT GGCGGGCACG GTGGCCCCAG GTGGGGTGAG TGGCCCTGCC
    CCGGCGCAGC TGGGGGCCCC GGCCCTTGGG GGGCAGCAGT CAGTGTCCAA CAAACTCCTG
    GCCTGGAGCG GGGTCCTCGA ATGGCAGGAG AAGCCCAAAC CTGCCTCCGT GGACGCCAAC
    ACCAAGCTGA CGCGATCGCT GCCTTGCCAG GTCTATGTGA ATCACGGCGA GAACTTAAAG
    ACTGAGCAGT GGCCTCAGAA GCTGATCATG CAGCTCATCC CACAGCAGTT GCTGACCACC
    CTGGGCCCTT TGTTCCGGAA CTCAAGGATG GTGCAGTTCC ATTTCACCAA CAAGGACCTG
    GAGTCCCTGA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCTGGCTG TGTGCACTTC
    CCCCACACGG CCCCCTGTGA GGTGCGTGTG CTCATGCTCC TGTACTCGTC CAAGAAGAAG
    ATTTTCATGG GCCTCATCCC CTATGACCAG AGCGGCTTCG TCAATGGCAT CCGGCAGGTC
    ATCACCAACC ACAAGCAAGT CCAGCAGCAG AAGCTGGAGC AGCAGCGTGT GATGGGGGCA
    CAGCAGGCAC CCCCTGGGCT GGGCCCCATT CTGGAGGACC AGGCGAGACC CTCACAGAAT
    CTGCTCCAGC TCCGCCCACC ACAGCCCCAA CCTCAGGGCA CTGTGGGGGC CTCTGCGGCC
    TCAGGACAGC CCCAGCCCCA AGGGGCTGCC CAGGCCCCCC CAGGTGCCCC CCAAGGTCCT
    CCTGGAGCAG CCCCTGGCCC CCCCCCTCCT GGACCCATCC TTCGGCCTCA GAACCCTGGG
    GCCAACCCCC AACTGCGGAG TCTCCTCCTC AACCCACCTC CGCCTCAGAC TGGGGTGCCC
    CCACCCCAAG CTTCCCTCCA CCACCTCCAG CCACCAGGGG CTCCAGCACT GTTGCCACCA
    CACCAGGGCC TGGGGCAACC CCAGCTGGGG CCCCCCCTCC TGCACCCACC ACCTGCCCAG
    TCCTGGCCCA CACAGCTTCC CCCGAGAGCT CCGCTGCCAG GTCAGATGCT GCTGAGCGGG
    GGTCCCCGGG GCCCGGTTCC CCAGCCGGGC CTGCAGCCCA GCGTCATGGA GGACGACATC
    CTCATGGATC TCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34928
    Clone ID Related Accession (Same CDS sequence) XM_541489.7 , XM_541489.6
    Accession Version XM_541489.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_541489.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGTCCCCG GGTCGGAGGG CCCGGCCCGC GCCGGGGGCC TCGTGGCTGA CGTGGTGTTT 
    GTGATCGAGG GTACAGCCAA CCTGGGGCCT TACTTCGAAG GGTTGCGCAA GCACTACCTG
    CTTCCGGCCA TCGAGTATTT TAATGGTGGT CCTCCTGCCG AGACGGACTT CGGAGGAGAC
    TATGGGGGTA CCCAGTACAG CCTCGTGGTG TTCAACACGG TGGACTGCGC TCCCGAGTCC
    TACGTACAGT GTCACGCTCC CACCAGCAGC GCCTATGAGT TTGTCACCTG GCTCGATGGC
    ATTAAGTTCA TGGGCGGGGG TGGTGAGAGT TGTAGCCTCA TCGCGGAAGG CCTCAGCACG
    GCCCTGCAGC TCTTCGATGA CTTCAAGAAG ATGCGGGAGC AGATCGGCCA GACACACCGC
    GTCTGCCTCC TCATCTGTAA CTCACCCCCG TACCTGCTCC CTGCCGTCGA GAGTACCACA
    TACTCTGGGT ACACCACAGA GAGCCTCGTG CAGAAGATAG GTGAGCAAGG GATCCACTTC
    TCCATCGTGT CTCCCCGGAA GCTGCCTGCC CTGAGGCTTC TGTTCGAGAA GGCAGCTCCA
    CCGGCCTTGC TGGAGCCGCT GCAGCCACCA ACAGATGTGA GCCAGGATCC CCGGCACATG
    GTGCTGGTGC GGGGGCTTGT GCTGCCAGTT GGGGGTGGCT CAGCCCCAGG CCCCCTGCAG
    CCAAAGCAGC CGGTCCCCCT GCCTCCAGCT CCACCCTCGG GTGCCTCACT CTCAGCAGCG
    CCCCAGCAGC CTCTGCCCCC CGTCCCTCAG CAGTACCAGG TCCCTGGGAA CCTGAGTGCA
    GCTCAGGTGG CTGCCCAGAA TGCAGTGGAA GCCGCCAAGA ACCAGAAGGC TGGGCTGGGC
    CCCCGCTTCT CACCCATTAA CCCTCTCCAA CAAGCTGCTC CAGGAGTGGG TCCCCCCTTC
    AGCCAGGCAC CGGCCCCACC ACTACCCCCG GGGCCCCCTG GCGCCCCCAA GCCACCCCCT
    GCTTCCCAGC CCAGCCTGGT CTCCACTGTG GCTCCTGGCC CAGGCCTGGC CCCCCCTGCA
    CAACCTGGGG CCCCATCCAT GGCGGGCACG GTGGCCCCAG GTGGGGTGAG TGGCCCTGCC
    CCGGCGCAGC TGGGGGCCCC GGCCCTTGGG GGGCAGCAGT CAGTGTCCAA CAAACTCCTG
    GCCTGGAGCG GGGTCCTCGA ATGGCAGGAG AAGCCCAAAC CTGCCTCCGT GGACGCCAAC
    ACCAAGCTGA CGCGATCGCT GCCTTGCCAG GTCTATGTGA ATCACGGCGA GAACTTAAAG
    ACTGAGCAGT GGCCTCAGAA GCTGATCATG CAGCTCATCC CACAGCAGTT GCTGACCACC
    CTGGGCCCTT TGTTCCGGAA CTCAAGGATG GTGCAGTTCC ATTTCACCAA CAAGGACCTG
    GAGTCCCTGA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCTGGCTG TGTGCACTTC
    CCCCACACGG CCCCCTGTGA GGTGCGTGTG CTCATGCTCC TGTACTCGTC CAAGAAGAAG
    ATTTTCATGG GCCTCATCCC CTATGACCAG AGCGGCTTCG TCAATGGCAT CCGGCAGGTC
    ATCACCAACC ACAAGCAAGT CCAGCAGCAG AAGCTGGAGC AGCAGCGTGT GATGGGGGCA
    CAGCAGGCAC CCCCTGGGCT GGGCCCCATT CTGGAGGACC AGGCGAGACC CTCACAGAAT
    CTGCTCCAGC TCCGCCCACC ACAGCCCCAA CCTCAGGGCA CTGTGGGGGC CTCTGCGGCC
    TCAGGACAGC CCCAGCCCCA AGGGGCTGCC CAGGCCCCCC CAGGTGCCCC CCAAGGTCCT
    CCTGGAGCAG CCCCTGGCCC CCCCCCTCCT GGACCCATCC TTCGGCCTCA GAACCCTGGG
    GCCAACCCCC AACTGCGGAG TCTCCTCCTC AACCCACCTC CGCCTCAGAC TGGGGTGCCC
    CCACCCCAAG CTTCCCTCCA CCACCTCCAG CCACCAGGGG CTCCAGCACT GTTGCCACCA
    CACCAGGGCC TGGGGCAACC CCAGCTGGGG CCCCCCCTCC TGCACCCACC ACCTGCCCAG
    TCCTGGCCCA CACAGCTTCC CCCGAGAGCT CCGCTGCCAG GTCAGATGCT GCTGAGCGGG
    GGTCCCCGGG GCCCGGTTCC CCAGCCGGGC CTGCAGCCCA GCGTCATGGA GGACGACATC
    CTCATGGATC TCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_541489.5
    CDS77..2314
    Translation

    Target ORF information:

    RefSeq Version XM_541489.6
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mediator complex subunit 25 (MED25), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_541489.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGTCCCCG GGTCGGAGGG CCCGGCCCGC GCCGGGGGCC TCGTGGCTGA CGTGGTGTTT 
    GTGATCGAGG GTACAGCCAA CCTGGGGCCT TACTTCGAAG GGTTGCGCAA GCACTACCTG
    CTTCCGGCCA TCGAGTATTT TAATGGTGGT CCTCCTGCCG AGACGGACTT CGGAGGAGAC
    TATGGGGGTA CCCAGTACAG CCTCGTGGTG TTCAACACGG TGGACTGCGC TCCCGAGTCC
    TACGTACAGT GTCACGCTCC CACCAGCAGC GCCTATGAGT TTGTCACCTG GCTCGATGGC
    ATTAAGTTCA TGGGCGGGGG TGGTGAGAGT TGTAGCCTCA TCGCGGAAGG CCTCAGCACG
    GCCCTGCAGC TCTTCGATGA CTTCAAGAAG ATGCGGGAGC AGATCGGCCA GACACACCGC
    GTCTGCCTCC TCATCTGTAA CTCACCCCCG TACCTGCTCC CTGCCGTCGA GAGTACCACA
    TACTCTGGGT ACACCACAGA GAGCCTCGTG CAGAAGATAG GTGAGCAAGG GATCCACTTC
    TCCATCGTGT CTCCCCGGAA GCTGCCTGCC CTGAGGCTTC TGTTCGAGAA GGCAGCTCCA
    CCGGCCTTGC TGGAGCCGCT GCAGCCACCA ACAGATGTGA GCCAGGATCC CCGGCACATG
    GTGCTGGTGC GGGGGCTTGT GCTGCCAGTT GGGGGTGGCT CAGCCCCAGG CCCCCTGCAG
    CCAAAGCAGC CGGTCCCCCT GCCTCCAGCT CCACCCTCGG GTGCCTCACT CTCAGCAGCG
    CCCCAGCAGC CTCTGCCCCC CGTCCCTCAG CAGTACCAGG TCCCTGGGAA CCTGAGTGCA
    GCTCAGGTGG CTGCCCAGAA TGCAGTGGAA GCCGCCAAGA ACCAGAAGGC TGGGCTGGGC
    CCCCGCTTCT CACCCATTAA CCCTCTCCAA CAAGCTGCTC CAGGAGTGGG TCCCCCCTTC
    AGCCAGGCAC CGGCCCCACC ACTACCCCCG GGGCCCCCTG GCGCCCCCAA GCCACCCCCT
    GCTTCCCAGC CCAGCCTGGT CTCCACTGTG GCTCCTGGCC CAGGCCTGGC CCCCCCTGCA
    CAACCTGGGG CCCCATCCAT GGCGGGCACG GTGGCCCCAG GTGGGGTGAG TGGCCCTGCC
    CCGGCGCAGC TGGGGGCCCC GGCCCTTGGG GGGCAGCAGT CAGTGTCCAA CAAACTCCTG
    GCCTGGAGCG GGGTCCTCGA ATGGCAGGAG AAGCCCAAAC CTGCCTCCGT GGACGCCAAC
    ACCAAGCTGA CGCGATCGCT GCCTTGCCAG GTCTATGTGA ATCACGGCGA GAACTTAAAG
    ACTGAGCAGT GGCCTCAGAA GCTGATCATG CAGCTCATCC CACAGCAGTT GCTGACCACC
    CTGGGCCCTT TGTTCCGGAA CTCAAGGATG GTGCAGTTCC ATTTCACCAA CAAGGACCTG
    GAGTCCCTGA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCTGGCTG TGTGCACTTC
    CCCCACACGG CCCCCTGTGA GGTGCGTGTG CTCATGCTCC TGTACTCGTC CAAGAAGAAG
    ATTTTCATGG GCCTCATCCC CTATGACCAG AGCGGCTTCG TCAATGGCAT CCGGCAGGTC
    ATCACCAACC ACAAGCAAGT CCAGCAGCAG AAGCTGGAGC AGCAGCGTGT GATGGGGGCA
    CAGCAGGCAC CCCCTGGGCT GGGCCCCATT CTGGAGGACC AGGCGAGACC CTCACAGAAT
    CTGCTCCAGC TCCGCCCACC ACAGCCCCAA CCTCAGGGCA CTGTGGGGGC CTCTGCGGCC
    TCAGGACAGC CCCAGCCCCA AGGGGCTGCC CAGGCCCCCC CAGGTGCCCC CCAAGGTCCT
    CCTGGAGCAG CCCCTGGCCC CCCCCCTCCT GGACCCATCC TTCGGCCTCA GAACCCTGGG
    GCCAACCCCC AACTGCGGAG TCTCCTCCTC AACCCACCTC CGCCTCAGAC TGGGGTGCCC
    CCACCCCAAG CTTCCCTCCA CCACCTCCAG CCACCAGGGG CTCCAGCACT GTTGCCACCA
    CACCAGGGCC TGGGGCAACC CCAGCTGGGG CCCCCCCTCC TGCACCCACC ACCTGCCCAG
    TCCTGGCCCA CACAGCTTCC CCCGAGAGCT CCGCTGCCAG GTCAGATGCT GCTGAGCGGG
    GGTCCCCGGG GCCCGGTTCC CCAGCCGGGC CTGCAGCCCA GCGTCATGGA GGACGACATC
    CTCATGGATC TCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.