med25 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following med25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the med25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa00786 NM_001102796.1
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa00786 XM_031904755.1
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa00786 XM_031904754.1
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa30471 XM_012965886.3
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa30472 XM_031904756.1
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa30473 XM_031904757.1
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa30474 XM_031904759.1
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa30475 XM_012965890.3
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa45298 XM_018095221.1
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OXa45298 XM_018095222.1
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OXa45298 XM_018095223.1
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OXa45299 XM_018095224.1
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OXa45300 XM_018095225.1
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OXa45301 XM_018095226.1
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OXa45302 XM_018095227.1
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OXa45303 XM_018095228.1
Latest version!
Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OXa30475 XM_012965890.2 Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa30471 XM_012965886.2 Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa00786
    Clone ID Related Accession (Same CDS sequence) XM_031904754.1 , XM_031904755.1 , NM_001102796.1
    Accession Version NM_001102796.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC135482.1. ##Evidence-Data-START## Transcript exon combination :: BC135482.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTC AGGCCGGTTG CGTACACTTC
    CCGCACACAA CTCCATGTGA AGTCCGAGTC CTCATGCTCC TCTACTCCTC CAAAAAGAAG
    ATCTTCATGG GGCTCATCCC TAACGACCAG AGTGGCTTCG TCAACGGTAT CCGACAGGTG
    ATCACAAACC ACAAGCAAGT TCAGATGCAG AAGATTGACC AACAGCGAAA TATGGGAGCT
    GGCCAAGCTG TGGGCACTGG GAATGTTCCA GCCAACACCC AAGCCTTCCT CCAGAAACAG
    CCAGGGGCTC TGCCAGTGGC ACAGGCCGTA CCCCAACAGA TGCAAGGCCA GCAGGTAGCC
    CCGGGCATGC CCTCCATTAG CCAAGTCACT ATGATGGATG AGCAACAGAG AACACAGAAT
    TTGTTACACA TCAGAGTACA GCAACCACAG CAAGCAGCCT CTCAGGCACC ACCACAAGCC
    ACACAAACCA CCGTTCAAGC ACCTGGCCAG CCTCAGAACC CACAGCCAGG AGCGATGCTC
    CGACCACAAA ACCCTGGTGC CAACCCACAG CTACGCAACC TCCTCCTGAG CCAGCAGCCT
    CCACAGACCA GTGTCCCACA AACACAGCAG CCTTTGCACC ATATGCAGCA AGCGGCTCAG
    GGCATGCTTC CCCATCAGGC CATGGGCCAA CAGATGCAGC ACCAGGCTCC CGGCCAGCAG
    CAACTCCAAC TCCCGGGGCA AACACTTATG CACCAAGCCC CCGCGCAGCA ATGGGGGAAC
    CAGATGCAAC AGAGAGCACC AATTCCAGGG ACACTGATGA TGAGCGCGGG ACCTCGAGGC
    CCTGTCCCTC AGCAAGGCCT ACAGCAAGTG CAGGCTCAGA GTGTCATGGA AGACGACATC
    CTAATGGACC TTATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001096266.1
    CDS143..2560
    Translation

    Target ORF information:

    RefSeq Version NM_001102796.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102796.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTC AGGCCGGTTG CGTACACTTC
    CCGCACACAA CTCCATGTGA AGTCCGAGTC CTCATGCTCC TCTACTCCTC CAAAAAGAAG
    ATCTTCATGG GGCTCATCCC TAACGACCAG AGTGGCTTCG TCAACGGTAT CCGACAGGTG
    ATCACAAACC ACAAGCAAGT TCAGATGCAG AAGATTGACC AACAGCGAAA TATGGGAGCT
    GGCCAAGCTG TGGGCACTGG GAATGTTCCA GCCAACACCC AAGCCTTCCT CCAGAAACAG
    CCAGGGGCTC TGCCAGTGGC ACAGGCCGTA CCCCAACAGA TGCAAGGCCA GCAGGTAGCC
    CCGGGCATGC CCTCCATTAG CCAAGTCACT ATGATGGATG AGCAACAGAG AACACAGAAT
    TTGTTACACA TCAGAGTACA GCAACCACAG CAAGCAGCCT CTCAGGCACC ACCACAAGCC
    ACACAAACCA CCGTTCAAGC ACCTGGCCAG CCTCAGAACC CACAGCCAGG AGCGATGCTC
    CGACCACAAA ACCCTGGTGC CAACCCACAG CTACGCAACC TCCTCCTGAG CCAGCAGCCT
    CCACAGACCA GTGTCCCACA AACACAGCAG CCTTTGCACC ATATGCAGCA AGCGGCTCAG
    GGCATGCTTC CCCATCAGGC CATGGGCCAA CAGATGCAGC ACCAGGCTCC CGGCCAGCAG
    CAACTCCAAC TCCCGGGGCA AACACTTATG CACCAAGCCC CCGCGCAGCA ATGGGGGAAC
    CAGATGCAAC AGAGAGCACC AATTCCAGGG ACACTGATGA TGAGCGCGGG ACCTCGAGGC
    CCTGTCCCTC AGCAAGGCCT ACAGCAAGTG CAGGCTCAGA GTGTCATGGA AGACGACATC
    CTAATGGACC TTATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30471
    Clone ID Related Accession (Same CDS sequence) XM_012965886.2 , XM_012965886.3
    Accession Version XM_012965886.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTG CCGGTTGCGT ACACTTCCCG
    CACACAACTC CATGTGAAGT CCGAGTCCTC ATGCTCCTCT ACTCCTCCAA AAAGAAGATC
    TTCATGGGGC TCATCCCTAA CGACCAGAGT GGCTTCGTCA ACGGTATCCG ACAGGTGATC
    ACAAACCACA AGCAAGTTCA GATGCAGAAG ATTGACCAAC AGCGAAATAT GGGAGCTGGC
    CAAGCTGTGG GCACTGGGAA TGTTCCAGCC AACACCCAAG CCTTCCTCCA GAAACAGCCA
    GGGGCTCTGC CAGTGGCACA GGCCGTACCC CAACAGATGC AAGGCCAGCA GGTAGCCCCG
    GGCATGCCCT CCATTAGCCA AGTCACTATG ATGGATGAGC AACAGAGAAC ACAGAATTTG
    TTACACATCA GAGTACAGCA ACCACAGCAA GCAGCCTCTC AGGCACCACC ACAAGCCACA
    CAAACCACCG TTCAAGCACC TGGCCAGCCT CAGAACCCAC AGCCAGGAGC GATGCTCCGA
    CCACAAAACC CTGGTGCCAA CCCACAGCTA CGCAACCTCC TCCTGAGCCA GCAGCCTCCA
    CAGACCAGTG TCCCACAAAC ACAGCAGCCT TTGCACCATA TGCAGCAAGC GGCTCAGGGC
    ATGCTTCCCC ATCAGGCCAT GGGCCAACAG ATGCAGCACC AGGCTCCCGG CCAGCAGCAA
    CTCCAACTCC CGGGGCAAAC ACTTATGCAC CAAGCCCCCG CGCAGCAATG GGGGAACCAG
    ATGCAACAGA GAGCACCAAT TCCAGGGACA CTGATGATGA GCGCGGGACC TCGAGGCCCT
    GTCCCTCAGC AAGGCCTACA GCAAGTGCAG GCTCAGAGTG TCATGGAAGA CGACATCCTA
    ATGGACCTTA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821340.1
    CDS189..2603
    Translation

    Target ORF information:

    RefSeq Version XM_012965886.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965886.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTG CCGGTTGCGT ACACTTCCCG
    CACACAACTC CATGTGAAGT CCGAGTCCTC ATGCTCCTCT ACTCCTCCAA AAAGAAGATC
    TTCATGGGGC TCATCCCTAA CGACCAGAGT GGCTTCGTCA ACGGTATCCG ACAGGTGATC
    ACAAACCACA AGCAAGTTCA GATGCAGAAG ATTGACCAAC AGCGAAATAT GGGAGCTGGC
    CAAGCTGTGG GCACTGGGAA TGTTCCAGCC AACACCCAAG CCTTCCTCCA GAAACAGCCA
    GGGGCTCTGC CAGTGGCACA GGCCGTACCC CAACAGATGC AAGGCCAGCA GGTAGCCCCG
    GGCATGCCCT CCATTAGCCA AGTCACTATG ATGGATGAGC AACAGAGAAC ACAGAATTTG
    TTACACATCA GAGTACAGCA ACCACAGCAA GCAGCCTCTC AGGCACCACC ACAAGCCACA
    CAAACCACCG TTCAAGCACC TGGCCAGCCT CAGAACCCAC AGCCAGGAGC GATGCTCCGA
    CCACAAAACC CTGGTGCCAA CCCACAGCTA CGCAACCTCC TCCTGAGCCA GCAGCCTCCA
    CAGACCAGTG TCCCACAAAC ACAGCAGCCT TTGCACCATA TGCAGCAAGC GGCTCAGGGC
    ATGCTTCCCC ATCAGGCCAT GGGCCAACAG ATGCAGCACC AGGCTCCCGG CCAGCAGCAA
    CTCCAACTCC CGGGGCAAAC ACTTATGCAC CAAGCCCCCG CGCAGCAATG GGGGAACCAG
    ATGCAACAGA GAGCACCAAT TCCAGGGACA CTGATGATGA GCGCGGGACC TCGAGGCCCT
    GTCCCTCAGC AAGGCCTACA GCAAGTGCAG GCTCAGAGTG TCATGGAAGA CGACATCCTA
    ATGGACCTTA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30475
    Clone ID Related Accession (Same CDS sequence) XM_012965890.3 , XM_012965890.2
    Accession Version XM_012965890.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965890.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTGCCG GTTGCGTACA CTTCCCGCAC
    ACAACTCCAT GTGAAGTCCG AGTCCTCATG CTCCTCTACT CCTCCAAAAA GAAGATCTTC
    ATGGGGCTCA TCCCTAACGA CCAGAGTGGC TTCGTCAACG GTATCCGACA GGTGATCACA
    AACCACAAGC AAGTTCAGAT GCAGAAGATT GACCAACAGC GAAATATGGG AGCTGGCCAA
    GCTGTGGGCA CTGGGAATGT TCCAGCCAAC ACCCAAGCCT TCCTCCAGAA ACAGCCAGGG
    GCTCTGCCAG TGGCACAGGC CGTACCCCAA CAGATGCAAG GCCAGCAGGT AGCCCCGGGC
    ATGCCCTCCA TTAGCCAAGT CACTATGATG GATGAGCAAC AGAGAACACA GAATTTGTTA
    CACATCAGAG TACAGCAACC ACAGCAAGCA GCCTCTCAGG CACCACCACA AGCCACACAA
    ACCACCGTTC AAGCACCTGG CCAGCCTCAG AACCCACAGC CAGGAGCGAT GCTCCGACCA
    CAAAACCCTG GTGCCAACCC ACAGCTACGC AACCTCCTCC TGAGCCAGCA GCCTCCACAG
    ACCAGTGTCC CACAAACACA GCAGCCTTTG CACCATATGC AGCAAGCGGC TCAGGGCATG
    CTTCCCCATC AGGCCATGGG CCAACAGATG CAGCACCAGG CTCCCGGCCA GCAGCAACTC
    CAACTCCCGG GGCAAACACT TATGCACCAA GCCCCCGCGC AGCAATGGGG GAACCAGATG
    CAACAGAGAG CACCAATTCC AGGGACACTG ATGATGAGCG CGGGACCTCG AGGCCCTGTC
    CCTCAGCAAG GCCTACAGCA AGTGCAGGCT CAGAGTGTCA TGGAAGACGA CATCCTAATG
    GACCTTATTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821344.1
    CDS189..2600
    Translation

    Target ORF information:

    RefSeq Version XM_012965890.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965890.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTGCCG GTTGCGTACA CTTCCCGCAC
    ACAACTCCAT GTGAAGTCCG AGTCCTCATG CTCCTCTACT CCTCCAAAAA GAAGATCTTC
    ATGGGGCTCA TCCCTAACGA CCAGAGTGGC TTCGTCAACG GTATCCGACA GGTGATCACA
    AACCACAAGC AAGTTCAGAT GCAGAAGATT GACCAACAGC GAAATATGGG AGCTGGCCAA
    GCTGTGGGCA CTGGGAATGT TCCAGCCAAC ACCCAAGCCT TCCTCCAGAA ACAGCCAGGG
    GCTCTGCCAG TGGCACAGGC CGTACCCCAA CAGATGCAAG GCCAGCAGGT AGCCCCGGGC
    ATGCCCTCCA TTAGCCAAGT CACTATGATG GATGAGCAAC AGAGAACACA GAATTTGTTA
    CACATCAGAG TACAGCAACC ACAGCAAGCA GCCTCTCAGG CACCACCACA AGCCACACAA
    ACCACCGTTC AAGCACCTGG CCAGCCTCAG AACCCACAGC CAGGAGCGAT GCTCCGACCA
    CAAAACCCTG GTGCCAACCC ACAGCTACGC AACCTCCTCC TGAGCCAGCA GCCTCCACAG
    ACCAGTGTCC CACAAACACA GCAGCCTTTG CACCATATGC AGCAAGCGGC TCAGGGCATG
    CTTCCCCATC AGGCCATGGG CCAACAGATG CAGCACCAGG CTCCCGGCCA GCAGCAACTC
    CAACTCCCGG GGCAAACACT TATGCACCAA GCCCCCGCGC AGCAATGGGG GAACCAGATG
    CAACAGAGAG CACCAATTCC AGGGACACTG ATGATGAGCG CGGGACCTCG AGGCCCTGTC
    CCTCAGCAAG GCCTACAGCA AGTGCAGGCT CAGAGTGTCA TGGAAGACGA CATCCTAATG
    GACCTTATTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa00786
    Clone ID Related Accession (Same CDS sequence) XM_031904754.1 , XM_031904755.1 , NM_001102796.1
    Accession Version XM_031904754.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTC AGGCCGGTTG CGTACACTTC
    CCGCACACAA CTCCATGTGA AGTCCGAGTC CTCATGCTCC TCTACTCCTC CAAAAAGAAG
    ATCTTCATGG GGCTCATCCC TAACGACCAG AGTGGCTTCG TCAACGGTAT CCGACAGGTG
    ATCACAAACC ACAAGCAAGT TCAGATGCAG AAGATTGACC AACAGCGAAA TATGGGAGCT
    GGCCAAGCTG TGGGCACTGG GAATGTTCCA GCCAACACCC AAGCCTTCCT CCAGAAACAG
    CCAGGGGCTC TGCCAGTGGC ACAGGCCGTA CCCCAACAGA TGCAAGGCCA GCAGGTAGCC
    CCGGGCATGC CCTCCATTAG CCAAGTCACT ATGATGGATG AGCAACAGAG AACACAGAAT
    TTGTTACACA TCAGAGTACA GCAACCACAG CAAGCAGCCT CTCAGGCACC ACCACAAGCC
    ACACAAACCA CCGTTCAAGC ACCTGGCCAG CCTCAGAACC CACAGCCAGG AGCGATGCTC
    CGACCACAAA ACCCTGGTGC CAACCCACAG CTACGCAACC TCCTCCTGAG CCAGCAGCCT
    CCACAGACCA GTGTCCCACA AACACAGCAG CCTTTGCACC ATATGCAGCA AGCGGCTCAG
    GGCATGCTTC CCCATCAGGC CATGGGCCAA CAGATGCAGC ACCAGGCTCC CGGCCAGCAG
    CAACTCCAAC TCCCGGGGCA AACACTTATG CACCAAGCCC CCGCGCAGCA ATGGGGGAAC
    CAGATGCAAC AGAGAGCACC AATTCCAGGG ACACTGATGA TGAGCGCGGG ACCTCGAGGC
    CCTGTCCCTC AGCAAGGCCT ACAGCAAGTG CAGGCTCAGA GTGTCATGGA AGACGACATC
    CTAATGGACC TTATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760614.1
    CDS276..2693
    Translation

    Target ORF information:

    RefSeq Version XM_031904754.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904754.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTC AGGCCGGTTG CGTACACTTC
    CCGCACACAA CTCCATGTGA AGTCCGAGTC CTCATGCTCC TCTACTCCTC CAAAAAGAAG
    ATCTTCATGG GGCTCATCCC TAACGACCAG AGTGGCTTCG TCAACGGTAT CCGACAGGTG
    ATCACAAACC ACAAGCAAGT TCAGATGCAG AAGATTGACC AACAGCGAAA TATGGGAGCT
    GGCCAAGCTG TGGGCACTGG GAATGTTCCA GCCAACACCC AAGCCTTCCT CCAGAAACAG
    CCAGGGGCTC TGCCAGTGGC ACAGGCCGTA CCCCAACAGA TGCAAGGCCA GCAGGTAGCC
    CCGGGCATGC CCTCCATTAG CCAAGTCACT ATGATGGATG AGCAACAGAG AACACAGAAT
    TTGTTACACA TCAGAGTACA GCAACCACAG CAAGCAGCCT CTCAGGCACC ACCACAAGCC
    ACACAAACCA CCGTTCAAGC ACCTGGCCAG CCTCAGAACC CACAGCCAGG AGCGATGCTC
    CGACCACAAA ACCCTGGTGC CAACCCACAG CTACGCAACC TCCTCCTGAG CCAGCAGCCT
    CCACAGACCA GTGTCCCACA AACACAGCAG CCTTTGCACC ATATGCAGCA AGCGGCTCAG
    GGCATGCTTC CCCATCAGGC CATGGGCCAA CAGATGCAGC ACCAGGCTCC CGGCCAGCAG
    CAACTCCAAC TCCCGGGGCA AACACTTATG CACCAAGCCC CCGCGCAGCA ATGGGGGAAC
    CAGATGCAAC AGAGAGCACC AATTCCAGGG ACACTGATGA TGAGCGCGGG ACCTCGAGGC
    CCTGTCCCTC AGCAAGGCCT ACAGCAAGTG CAGGCTCAGA GTGTCATGGA AGACGACATC
    CTAATGGACC TTATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa00786
    Clone ID Related Accession (Same CDS sequence) XM_031904754.1 , XM_031904755.1 , NM_001102796.1
    Accession Version XM_031904755.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTC AGGCCGGTTG CGTACACTTC
    CCGCACACAA CTCCATGTGA AGTCCGAGTC CTCATGCTCC TCTACTCCTC CAAAAAGAAG
    ATCTTCATGG GGCTCATCCC TAACGACCAG AGTGGCTTCG TCAACGGTAT CCGACAGGTG
    ATCACAAACC ACAAGCAAGT TCAGATGCAG AAGATTGACC AACAGCGAAA TATGGGAGCT
    GGCCAAGCTG TGGGCACTGG GAATGTTCCA GCCAACACCC AAGCCTTCCT CCAGAAACAG
    CCAGGGGCTC TGCCAGTGGC ACAGGCCGTA CCCCAACAGA TGCAAGGCCA GCAGGTAGCC
    CCGGGCATGC CCTCCATTAG CCAAGTCACT ATGATGGATG AGCAACAGAG AACACAGAAT
    TTGTTACACA TCAGAGTACA GCAACCACAG CAAGCAGCCT CTCAGGCACC ACCACAAGCC
    ACACAAACCA CCGTTCAAGC ACCTGGCCAG CCTCAGAACC CACAGCCAGG AGCGATGCTC
    CGACCACAAA ACCCTGGTGC CAACCCACAG CTACGCAACC TCCTCCTGAG CCAGCAGCCT
    CCACAGACCA GTGTCCCACA AACACAGCAG CCTTTGCACC ATATGCAGCA AGCGGCTCAG
    GGCATGCTTC CCCATCAGGC CATGGGCCAA CAGATGCAGC ACCAGGCTCC CGGCCAGCAG
    CAACTCCAAC TCCCGGGGCA AACACTTATG CACCAAGCCC CCGCGCAGCA ATGGGGGAAC
    CAGATGCAAC AGAGAGCACC AATTCCAGGG ACACTGATGA TGAGCGCGGG ACCTCGAGGC
    CCTGTCCCTC AGCAAGGCCT ACAGCAAGTG CAGGCTCAGA GTGTCATGGA AGACGACATC
    CTAATGGACC TTATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760615.1
    CDS273..2690
    Translation

    Target ORF information:

    RefSeq Version XM_031904755.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904755.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTC AGGCCGGTTG CGTACACTTC
    CCGCACACAA CTCCATGTGA AGTCCGAGTC CTCATGCTCC TCTACTCCTC CAAAAAGAAG
    ATCTTCATGG GGCTCATCCC TAACGACCAG AGTGGCTTCG TCAACGGTAT CCGACAGGTG
    ATCACAAACC ACAAGCAAGT TCAGATGCAG AAGATTGACC AACAGCGAAA TATGGGAGCT
    GGCCAAGCTG TGGGCACTGG GAATGTTCCA GCCAACACCC AAGCCTTCCT CCAGAAACAG
    CCAGGGGCTC TGCCAGTGGC ACAGGCCGTA CCCCAACAGA TGCAAGGCCA GCAGGTAGCC
    CCGGGCATGC CCTCCATTAG CCAAGTCACT ATGATGGATG AGCAACAGAG AACACAGAAT
    TTGTTACACA TCAGAGTACA GCAACCACAG CAAGCAGCCT CTCAGGCACC ACCACAAGCC
    ACACAAACCA CCGTTCAAGC ACCTGGCCAG CCTCAGAACC CACAGCCAGG AGCGATGCTC
    CGACCACAAA ACCCTGGTGC CAACCCACAG CTACGCAACC TCCTCCTGAG CCAGCAGCCT
    CCACAGACCA GTGTCCCACA AACACAGCAG CCTTTGCACC ATATGCAGCA AGCGGCTCAG
    GGCATGCTTC CCCATCAGGC CATGGGCCAA CAGATGCAGC ACCAGGCTCC CGGCCAGCAG
    CAACTCCAAC TCCCGGGGCA AACACTTATG CACCAAGCCC CCGCGCAGCA ATGGGGGAAC
    CAGATGCAAC AGAGAGCACC AATTCCAGGG ACACTGATGA TGAGCGCGGG ACCTCGAGGC
    CCTGTCCCTC AGCAAGGCCT ACAGCAAGTG CAGGCTCAGA GTGTCATGGA AGACGACATC
    CTAATGGACC TTATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30471
    Clone ID Related Accession (Same CDS sequence) XM_012965886.2 , XM_012965886.3
    Accession Version XM_012965886.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965886.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTG CCGGTTGCGT ACACTTCCCG
    CACACAACTC CATGTGAAGT CCGAGTCCTC ATGCTCCTCT ACTCCTCCAA AAAGAAGATC
    TTCATGGGGC TCATCCCTAA CGACCAGAGT GGCTTCGTCA ACGGTATCCG ACAGGTGATC
    ACAAACCACA AGCAAGTTCA GATGCAGAAG ATTGACCAAC AGCGAAATAT GGGAGCTGGC
    CAAGCTGTGG GCACTGGGAA TGTTCCAGCC AACACCCAAG CCTTCCTCCA GAAACAGCCA
    GGGGCTCTGC CAGTGGCACA GGCCGTACCC CAACAGATGC AAGGCCAGCA GGTAGCCCCG
    GGCATGCCCT CCATTAGCCA AGTCACTATG ATGGATGAGC AACAGAGAAC ACAGAATTTG
    TTACACATCA GAGTACAGCA ACCACAGCAA GCAGCCTCTC AGGCACCACC ACAAGCCACA
    CAAACCACCG TTCAAGCACC TGGCCAGCCT CAGAACCCAC AGCCAGGAGC GATGCTCCGA
    CCACAAAACC CTGGTGCCAA CCCACAGCTA CGCAACCTCC TCCTGAGCCA GCAGCCTCCA
    CAGACCAGTG TCCCACAAAC ACAGCAGCCT TTGCACCATA TGCAGCAAGC GGCTCAGGGC
    ATGCTTCCCC ATCAGGCCAT GGGCCAACAG ATGCAGCACC AGGCTCCCGG CCAGCAGCAA
    CTCCAACTCC CGGGGCAAAC ACTTATGCAC CAAGCCCCCG CGCAGCAATG GGGGAACCAG
    ATGCAACAGA GAGCACCAAT TCCAGGGACA CTGATGATGA GCGCGGGACC TCGAGGCCCT
    GTCCCTCAGC AAGGCCTACA GCAAGTGCAG GCTCAGAGTG TCATGGAAGA CGACATCCTA
    ATGGACCTTA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821340.1
    CDS192..2606
    Translation

    Target ORF information:

    RefSeq Version XM_012965886.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965886.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTG CCGGTTGCGT ACACTTCCCG
    CACACAACTC CATGTGAAGT CCGAGTCCTC ATGCTCCTCT ACTCCTCCAA AAAGAAGATC
    TTCATGGGGC TCATCCCTAA CGACCAGAGT GGCTTCGTCA ACGGTATCCG ACAGGTGATC
    ACAAACCACA AGCAAGTTCA GATGCAGAAG ATTGACCAAC AGCGAAATAT GGGAGCTGGC
    CAAGCTGTGG GCACTGGGAA TGTTCCAGCC AACACCCAAG CCTTCCTCCA GAAACAGCCA
    GGGGCTCTGC CAGTGGCACA GGCCGTACCC CAACAGATGC AAGGCCAGCA GGTAGCCCCG
    GGCATGCCCT CCATTAGCCA AGTCACTATG ATGGATGAGC AACAGAGAAC ACAGAATTTG
    TTACACATCA GAGTACAGCA ACCACAGCAA GCAGCCTCTC AGGCACCACC ACAAGCCACA
    CAAACCACCG TTCAAGCACC TGGCCAGCCT CAGAACCCAC AGCCAGGAGC GATGCTCCGA
    CCACAAAACC CTGGTGCCAA CCCACAGCTA CGCAACCTCC TCCTGAGCCA GCAGCCTCCA
    CAGACCAGTG TCCCACAAAC ACAGCAGCCT TTGCACCATA TGCAGCAAGC GGCTCAGGGC
    ATGCTTCCCC ATCAGGCCAT GGGCCAACAG ATGCAGCACC AGGCTCCCGG CCAGCAGCAA
    CTCCAACTCC CGGGGCAAAC ACTTATGCAC CAAGCCCCCG CGCAGCAATG GGGGAACCAG
    ATGCAACAGA GAGCACCAAT TCCAGGGACA CTGATGATGA GCGCGGGACC TCGAGGCCCT
    GTCCCTCAGC AAGGCCTACA GCAAGTGCAG GCTCAGAGTG TCATGGAAGA CGACATCCTA
    ATGGACCTTA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30472
    Clone ID Related Accession (Same CDS sequence) XM_031904756.1
    Accession Version XM_031904756.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGGTT CCTCAGCAGA ATACTTTGAC TACAGCACAT
    GCTGCTGCCC AGTCTGCGGT TGAAGCTGCA AAGAATCAGA AAAATAACCT CCCAAATCGC
    TTTCCACTTC CAAACCTTAA TCAGATGCCA CCTACATCGG CTGTTGGTAC TCCATTTAAC
    CAACCGCCGC CCCCTGCAAT GCCTCAGAAT ACCTCAGTCC CCAAGATGGT TACTTCCACT
    GCATCCTTAA TGACGCCTGC TTCTCAACCC AGTCTTGTCA CCACGGTAAC CACAGGTCCT
    GGACCAGCGC CTGTACAATT GCAGCAGCAG GGAGCTCAGC AACAGCCTGT TCCGCCATCT
    ATGCCCATAA CAGGAGCTGC TGGGGGAGTT CAAGCCCCAC AACCCTCACA GCCACAGATT
    GGAACAGCAC AGTTGCCATG CACACAGACA CCTGTTAATG GCAATAAATT GCTTGCCTGG
    AGTGGGGTGC TCGAGTGGCA AGAGAAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTCAGG CCGGTTGCGT ACACTTCCCG
    CACACAACTC CATGTGAAGT CCGAGTCCTC ATGCTCCTCT ACTCCTCCAA AAAGAAGATC
    TTCATGGGGC TCATCCCTAA CGACCAGAGT GGCTTCGTCA ACGGTATCCG ACAGGTGATC
    ACAAACCACA AGCAAGTTCA GATGCAGAAG ATTGACCAAC AGCGAAATAT GGGAGCTGGC
    CAAGCTGTGG GCACTGGGAA TGTTCCAGCC AACACCCAAG CCTTCCTCCA GAAACAGCCA
    GGGGCTCTGC CAGTGGCACA GGCCGTACCC CAACAGATGC AAGGCCAGCA GGTAGCCCCG
    GGCATGCCCT CCATTAGCCA AGTCACTATG ATGGATGAGC AACAGAGAAC ACAGAATTTG
    TTACACATCA GAGTACAGCA ACCACAGCAA GCAGCCTCTC AGGCACCACC ACAAGCCACA
    CAAACCACCG TTCAAGCACC TGGCCAGCCT CAGAACCCAC AGCCAGGAGC GATGCTCCGA
    CCACAAAACC CTGGTGCCAA CCCACAGCTA CGCAACCTCC TCCTGAGCCA GCAGCCTCCA
    CAGACCAGTG TCCCACAAAC ACAGCAGCCT TTGCACCATA TGCAGCAAGC GGCTCAGGGC
    ATGCTTCCCC ATCAGGCCAT GGGCCAACAG ATGCAGCACC AGGCTCCCGG CCAGCAGCAA
    CTCCAACTCC CGGGGCAAAC ACTTATGCAC CAAGCCCCCG CGCAGCAATG GGGGAACCAG
    ATGCAACAGA GAGCACCAAT TCCAGGGACA CTGATGATGA GCGCGGGACC TCGAGGCCCT
    GTCCCTCAGC AAGGCCTACA GCAAGTGCAG GCTCAGAGTG TCATGGAAGA CGACATCCTA
    ATGGACCTTA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760616.1
    CDS193..2607
    Translation

    Target ORF information:

    RefSeq Version XM_031904756.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904756.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGGTT CCTCAGCAGA ATACTTTGAC TACAGCACAT
    GCTGCTGCCC AGTCTGCGGT TGAAGCTGCA AAGAATCAGA AAAATAACCT CCCAAATCGC
    TTTCCACTTC CAAACCTTAA TCAGATGCCA CCTACATCGG CTGTTGGTAC TCCATTTAAC
    CAACCGCCGC CCCCTGCAAT GCCTCAGAAT ACCTCAGTCC CCAAGATGGT TACTTCCACT
    GCATCCTTAA TGACGCCTGC TTCTCAACCC AGTCTTGTCA CCACGGTAAC CACAGGTCCT
    GGACCAGCGC CTGTACAATT GCAGCAGCAG GGAGCTCAGC AACAGCCTGT TCCGCCATCT
    ATGCCCATAA CAGGAGCTGC TGGGGGAGTT CAAGCCCCAC AACCCTCACA GCCACAGATT
    GGAACAGCAC AGTTGCCATG CACACAGACA CCTGTTAATG GCAATAAATT GCTTGCCTGG
    AGTGGGGTGC TCGAGTGGCA AGAGAAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTCAGG CCGGTTGCGT ACACTTCCCG
    CACACAACTC CATGTGAAGT CCGAGTCCTC ATGCTCCTCT ACTCCTCCAA AAAGAAGATC
    TTCATGGGGC TCATCCCTAA CGACCAGAGT GGCTTCGTCA ACGGTATCCG ACAGGTGATC
    ACAAACCACA AGCAAGTTCA GATGCAGAAG ATTGACCAAC AGCGAAATAT GGGAGCTGGC
    CAAGCTGTGG GCACTGGGAA TGTTCCAGCC AACACCCAAG CCTTCCTCCA GAAACAGCCA
    GGGGCTCTGC CAGTGGCACA GGCCGTACCC CAACAGATGC AAGGCCAGCA GGTAGCCCCG
    GGCATGCCCT CCATTAGCCA AGTCACTATG ATGGATGAGC AACAGAGAAC ACAGAATTTG
    TTACACATCA GAGTACAGCA ACCACAGCAA GCAGCCTCTC AGGCACCACC ACAAGCCACA
    CAAACCACCG TTCAAGCACC TGGCCAGCCT CAGAACCCAC AGCCAGGAGC GATGCTCCGA
    CCACAAAACC CTGGTGCCAA CCCACAGCTA CGCAACCTCC TCCTGAGCCA GCAGCCTCCA
    CAGACCAGTG TCCCACAAAC ACAGCAGCCT TTGCACCATA TGCAGCAAGC GGCTCAGGGC
    ATGCTTCCCC ATCAGGCCAT GGGCCAACAG ATGCAGCACC AGGCTCCCGG CCAGCAGCAA
    CTCCAACTCC CGGGGCAAAC ACTTATGCAC CAAGCCCCCG CGCAGCAATG GGGGAACCAG
    ATGCAACAGA GAGCACCAAT TCCAGGGACA CTGATGATGA GCGCGGGACC TCGAGGCCCT
    GTCCCTCAGC AAGGCCTACA GCAAGTGCAG GCTCAGAGTG TCATGGAAGA CGACATCCTA
    ATGGACCTTA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30473
    Clone ID Related Accession (Same CDS sequence) XM_031904757.1
    Accession Version XM_031904757.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTCAGG CCGGTTGCGT ACACTTCCCG
    CACACAACTC CATGTGAAGT CCGAGTCCTC ATGCTCCTCT ACTCCTCCAA AAAGAAGATC
    TTCATGGGGC TCATCCCTAA CGACCAGAGT GGCTTCGTCA ACGGTATCCG ACAGGTGATC
    ACAAACCACA AGCAAGTTCA GATGCAGAAG ATTGACCAAC AGCGAAATAT GGGAGCTGGC
    CAAGCTGTGG GCACTGGGAA TGTTCCAGCC AACACCCAAG CCTTCCTCCA GAAACAGCCA
    GGGGCTCTGC CAGTGGCACA GGCCGTACCC CAACAGATGC AAGGCCAGCA GGTAGCCCCG
    GGCATGCCCT CCATTAGCCA AGTCACTATG ATGGATGAGC AACAGAGAAC ACAGAATTTG
    TTACACATCA GAGTACAGCA ACCACAGCAA GCAGCCTCTC AGGCACCACC ACAAGCCACA
    CAAACCACCG TTCAAGCACC TGGCCAGCCT CAGAACCCAC AGCCAGGAGC GATGCTCCGA
    CCACAAAACC CTGGTGCCAA CCCACAGCTA CGCAACCTCC TCCTGAGCCA GCAGCCTCCA
    CAGACCAGTG TCCCACAAAC ACAGCAGCCT TTGCACCATA TGCAGCAAGC GGCTCAGGGC
    ATGCTTCCCC ATCAGGCCAT GGGCCAACAG ATGCAGCACC AGGCTCCCGG CCAGCAGCAA
    CTCCAACTCC CGGGGCAAAC ACTTATGCAC CAAGCCCCCG CGCAGCAATG GGGGAACCAG
    ATGCAACAGA GAGCACCAAT TCCAGGGACA CTGATGATGA GCGCGGGACC TCGAGGCCCT
    GTCCCTCAGC AAGGCCTACA GCAAGTGCAG GCTCAGAGTG TCATGGAAGA CGACATCCTA
    ATGGACCTTA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760617.1
    CDS193..2607
    Translation

    Target ORF information:

    RefSeq Version XM_031904757.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904757.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTCAGG CCGGTTGCGT ACACTTCCCG
    CACACAACTC CATGTGAAGT CCGAGTCCTC ATGCTCCTCT ACTCCTCCAA AAAGAAGATC
    TTCATGGGGC TCATCCCTAA CGACCAGAGT GGCTTCGTCA ACGGTATCCG ACAGGTGATC
    ACAAACCACA AGCAAGTTCA GATGCAGAAG ATTGACCAAC AGCGAAATAT GGGAGCTGGC
    CAAGCTGTGG GCACTGGGAA TGTTCCAGCC AACACCCAAG CCTTCCTCCA GAAACAGCCA
    GGGGCTCTGC CAGTGGCACA GGCCGTACCC CAACAGATGC AAGGCCAGCA GGTAGCCCCG
    GGCATGCCCT CCATTAGCCA AGTCACTATG ATGGATGAGC AACAGAGAAC ACAGAATTTG
    TTACACATCA GAGTACAGCA ACCACAGCAA GCAGCCTCTC AGGCACCACC ACAAGCCACA
    CAAACCACCG TTCAAGCACC TGGCCAGCCT CAGAACCCAC AGCCAGGAGC GATGCTCCGA
    CCACAAAACC CTGGTGCCAA CCCACAGCTA CGCAACCTCC TCCTGAGCCA GCAGCCTCCA
    CAGACCAGTG TCCCACAAAC ACAGCAGCCT TTGCACCATA TGCAGCAAGC GGCTCAGGGC
    ATGCTTCCCC ATCAGGCCAT GGGCCAACAG ATGCAGCACC AGGCTCCCGG CCAGCAGCAA
    CTCCAACTCC CGGGGCAAAC ACTTATGCAC CAAGCCCCCG CGCAGCAATG GGGGAACCAG
    ATGCAACAGA GAGCACCAAT TCCAGGGACA CTGATGATGA GCGCGGGACC TCGAGGCCCT
    GTCCCTCAGC AAGGCCTACA GCAAGTGCAG GCTCAGAGTG TCATGGAAGA CGACATCCTA
    ATGGACCTTA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30474
    Clone ID Related Accession (Same CDS sequence) XM_031904759.1
    Accession Version XM_031904759.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGGTT CCTCAGCAGA ATACTTTGAC TACAGCACAT
    GCTGCTGCCC AGTCTGCGGT TGAAGCTGCA AAGAATCAGA AAAATAACCT CCCAAATCGC
    TTTCCACTTC CAAACCTTAA TCAGATGCCA CCTACATCGG CTGTTGGTAC TCCATTTAAC
    CAACCGCCGC CCCCTGCAAT GCCTCAGAAT ACCTCAGTCC CCAAGATGGT TACTTCCACT
    GCATCCTTAA TGACGCCTGC TTCTCAACCC AGTCTTGTCA CCACGGTAAC CACAGGTCCT
    GGACCAGCGC CTGTACAATT GCAGCAGCAG GGAGCTCAGC AACAGCCTGT TCCGCCATCT
    ATGCCCATAA CAGGAGCTGC TGGGGGAGTT CAAGCCCCAC AACCCTCACA GCCACAGATT
    GGAACAGCAC AGTTGCCATG CACACAGACA CCTGTTAATG GCAATAAATT GCTTGCCTGG
    AGTGGGGTGC TCGAGTGGCA AGAGAAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTGCCG GTTGCGTACA CTTCCCGCAC
    ACAACTCCAT GTGAAGTCCG AGTCCTCATG CTCCTCTACT CCTCCAAAAA GAAGATCTTC
    ATGGGGCTCA TCCCTAACGA CCAGAGTGGC TTCGTCAACG GTATCCGACA GGTGATCACA
    AACCACAAGC AAGTTCAGAT GCAGAAGATT GACCAACAGC GAAATATGGG AGCTGGCCAA
    GCTGTGGGCA CTGGGAATGT TCCAGCCAAC ACCCAAGCCT TCCTCCAGAA ACAGCCAGGG
    GCTCTGCCAG TGGCACAGGC CGTACCCCAA CAGATGCAAG GCCAGCAGGT AGCCCCGGGC
    ATGCCCTCCA TTAGCCAAGT CACTATGATG GATGAGCAAC AGAGAACACA GAATTTGTTA
    CACATCAGAG TACAGCAACC ACAGCAAGCA GCCTCTCAGG CACCACCACA AGCCACACAA
    ACCACCGTTC AAGCACCTGG CCAGCCTCAG AACCCACAGC CAGGAGCGAT GCTCCGACCA
    CAAAACCCTG GTGCCAACCC ACAGCTACGC AACCTCCTCC TGAGCCAGCA GCCTCCACAG
    ACCAGTGTCC CACAAACACA GCAGCCTTTG CACCATATGC AGCAAGCGGC TCAGGGCATG
    CTTCCCCATC AGGCCATGGG CCAACAGATG CAGCACCAGG CTCCCGGCCA GCAGCAACTC
    CAACTCCCGG GGCAAACACT TATGCACCAA GCCCCCGCGC AGCAATGGGG GAACCAGATG
    CAACAGAGAG CACCAATTCC AGGGACACTG ATGATGAGCG CGGGACCTCG AGGCCCTGTC
    CCTCAGCAAG GCCTACAGCA AGTGCAGGCT CAGAGTGTCA TGGAAGACGA CATCCTAATG
    GACCTTATTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760619.1
    CDS192..2603
    Translation

    Target ORF information:

    RefSeq Version XM_031904759.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904759.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGGTT CCTCAGCAGA ATACTTTGAC TACAGCACAT
    GCTGCTGCCC AGTCTGCGGT TGAAGCTGCA AAGAATCAGA AAAATAACCT CCCAAATCGC
    TTTCCACTTC CAAACCTTAA TCAGATGCCA CCTACATCGG CTGTTGGTAC TCCATTTAAC
    CAACCGCCGC CCCCTGCAAT GCCTCAGAAT ACCTCAGTCC CCAAGATGGT TACTTCCACT
    GCATCCTTAA TGACGCCTGC TTCTCAACCC AGTCTTGTCA CCACGGTAAC CACAGGTCCT
    GGACCAGCGC CTGTACAATT GCAGCAGCAG GGAGCTCAGC AACAGCCTGT TCCGCCATCT
    ATGCCCATAA CAGGAGCTGC TGGGGGAGTT CAAGCCCCAC AACCCTCACA GCCACAGATT
    GGAACAGCAC AGTTGCCATG CACACAGACA CCTGTTAATG GCAATAAATT GCTTGCCTGG
    AGTGGGGTGC TCGAGTGGCA AGAGAAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTGCCG GTTGCGTACA CTTCCCGCAC
    ACAACTCCAT GTGAAGTCCG AGTCCTCATG CTCCTCTACT CCTCCAAAAA GAAGATCTTC
    ATGGGGCTCA TCCCTAACGA CCAGAGTGGC TTCGTCAACG GTATCCGACA GGTGATCACA
    AACCACAAGC AAGTTCAGAT GCAGAAGATT GACCAACAGC GAAATATGGG AGCTGGCCAA
    GCTGTGGGCA CTGGGAATGT TCCAGCCAAC ACCCAAGCCT TCCTCCAGAA ACAGCCAGGG
    GCTCTGCCAG TGGCACAGGC CGTACCCCAA CAGATGCAAG GCCAGCAGGT AGCCCCGGGC
    ATGCCCTCCA TTAGCCAAGT CACTATGATG GATGAGCAAC AGAGAACACA GAATTTGTTA
    CACATCAGAG TACAGCAACC ACAGCAAGCA GCCTCTCAGG CACCACCACA AGCCACACAA
    ACCACCGTTC AAGCACCTGG CCAGCCTCAG AACCCACAGC CAGGAGCGAT GCTCCGACCA
    CAAAACCCTG GTGCCAACCC ACAGCTACGC AACCTCCTCC TGAGCCAGCA GCCTCCACAG
    ACCAGTGTCC CACAAACACA GCAGCCTTTG CACCATATGC AGCAAGCGGC TCAGGGCATG
    CTTCCCCATC AGGCCATGGG CCAACAGATG CAGCACCAGG CTCCCGGCCA GCAGCAACTC
    CAACTCCCGG GGCAAACACT TATGCACCAA GCCCCCGCGC AGCAATGGGG GAACCAGATG
    CAACAGAGAG CACCAATTCC AGGGACACTG ATGATGAGCG CGGGACCTCG AGGCCCTGTC
    CCTCAGCAAG GCCTACAGCA AGTGCAGGCT CAGAGTGTCA TGGAAGACGA CATCCTAATG
    GACCTTATTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30475
    Clone ID Related Accession (Same CDS sequence) XM_012965890.3 , XM_012965890.2
    Accession Version XM_012965890.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965890.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTGCCG GTTGCGTACA CTTCCCGCAC
    ACAACTCCAT GTGAAGTCCG AGTCCTCATG CTCCTCTACT CCTCCAAAAA GAAGATCTTC
    ATGGGGCTCA TCCCTAACGA CCAGAGTGGC TTCGTCAACG GTATCCGACA GGTGATCACA
    AACCACAAGC AAGTTCAGAT GCAGAAGATT GACCAACAGC GAAATATGGG AGCTGGCCAA
    GCTGTGGGCA CTGGGAATGT TCCAGCCAAC ACCCAAGCCT TCCTCCAGAA ACAGCCAGGG
    GCTCTGCCAG TGGCACAGGC CGTACCCCAA CAGATGCAAG GCCAGCAGGT AGCCCCGGGC
    ATGCCCTCCA TTAGCCAAGT CACTATGATG GATGAGCAAC AGAGAACACA GAATTTGTTA
    CACATCAGAG TACAGCAACC ACAGCAAGCA GCCTCTCAGG CACCACCACA AGCCACACAA
    ACCACCGTTC AAGCACCTGG CCAGCCTCAG AACCCACAGC CAGGAGCGAT GCTCCGACCA
    CAAAACCCTG GTGCCAACCC ACAGCTACGC AACCTCCTCC TGAGCCAGCA GCCTCCACAG
    ACCAGTGTCC CACAAACACA GCAGCCTTTG CACCATATGC AGCAAGCGGC TCAGGGCATG
    CTTCCCCATC AGGCCATGGG CCAACAGATG CAGCACCAGG CTCCCGGCCA GCAGCAACTC
    CAACTCCCGG GGCAAACACT TATGCACCAA GCCCCCGCGC AGCAATGGGG GAACCAGATG
    CAACAGAGAG CACCAATTCC AGGGACACTG ATGATGAGCG CGGGACCTCG AGGCCCTGTC
    CCTCAGCAAG GCCTACAGCA AGTGCAGGCT CAGAGTGTCA TGGAAGACGA CATCCTAATG
    GACCTTATTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821344.1
    CDS193..2604
    Translation

    Target ORF information:

    RefSeq Version XM_012965890.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965890.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTGCCG GTTGCGTACA CTTCCCGCAC
    ACAACTCCAT GTGAAGTCCG AGTCCTCATG CTCCTCTACT CCTCCAAAAA GAAGATCTTC
    ATGGGGCTCA TCCCTAACGA CCAGAGTGGC TTCGTCAACG GTATCCGACA GGTGATCACA
    AACCACAAGC AAGTTCAGAT GCAGAAGATT GACCAACAGC GAAATATGGG AGCTGGCCAA
    GCTGTGGGCA CTGGGAATGT TCCAGCCAAC ACCCAAGCCT TCCTCCAGAA ACAGCCAGGG
    GCTCTGCCAG TGGCACAGGC CGTACCCCAA CAGATGCAAG GCCAGCAGGT AGCCCCGGGC
    ATGCCCTCCA TTAGCCAAGT CACTATGATG GATGAGCAAC AGAGAACACA GAATTTGTTA
    CACATCAGAG TACAGCAACC ACAGCAAGCA GCCTCTCAGG CACCACCACA AGCCACACAA
    ACCACCGTTC AAGCACCTGG CCAGCCTCAG AACCCACAGC CAGGAGCGAT GCTCCGACCA
    CAAAACCCTG GTGCCAACCC ACAGCTACGC AACCTCCTCC TGAGCCAGCA GCCTCCACAG
    ACCAGTGTCC CACAAACACA GCAGCCTTTG CACCATATGC AGCAAGCGGC TCAGGGCATG
    CTTCCCCATC AGGCCATGGG CCAACAGATG CAGCACCAGG CTCCCGGCCA GCAGCAACTC
    CAACTCCCGG GGCAAACACT TATGCACCAA GCCCCCGCGC AGCAATGGGG GAACCAGATG
    CAACAGAGAG CACCAATTCC AGGGACACTG ATGATGAGCG CGGGACCTCG AGGCCCTGTC
    CCTCAGCAAG GCCTACAGCA AGTGCAGGCT CAGAGTGTCA TGGAAGACGA CATCCTAATG
    GACCTTATTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45298
    Clone ID Related Accession (Same CDS sequence) XM_018095222.1 , XM_018095221.1 , XM_018095223.1
    Accession Version XM_018095222.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTC AGGCCGGTTG CGTACACTTC
    CCGCACACAA CTCCATGTGA AGTCCGAGTC CTCATGCTCC TCTACTCCTC CAAAAAGAAG
    ATCTTCATGG GGCTCATCCC TAACGACCAG AGTGGCTTCG TCAACGGTAT CCGACAGGTG
    ATCACAAACC ACAAGCAAGT TCAGATGCAG AAGATTGACC AACAGCGAAA TGTACGAATG
    ATGGGAGCTG GCCAAGCTGT GGGCACTGGG AATGTTCCAG CCAACACCCA AGCCTTCCTC
    CAGAAACAGC CAGGGGCTCT GCCAGTGGCA CAGGCCGTAC CCCAACAGAT GCAAGGCCAG
    CAGGTAGCCC CGGGCATGCC CTCCATTAGC CAAGTCACTA TGATGGATGA GCAACAGAGA
    ACACAGAATT TGTTACACAT CAGAGTACAG CAACCACAGC AAGCAGCCTC TCAGGCACCA
    CCACAAGCCA CACAAACCAC CGTTCAAGCA CCTGGCCAGC CTCAGAACCC ACAGCCAGGA
    GCGATGCTCC GACCACAAAA CCCTGGTGCC AACCCACAGC TACGCAACCT CCTCCTGAGC
    CAGCAGCCTC CACAGACCAG TGTCCCACAA ACACAGCAGC CTTTGCACCA TATGCAGCAA
    GCGGCTCAGG GCATGCTTCC CCATCAGGCC ATGGGCCAAC AGATGCAGCA CCAGGCTCCC
    GGCCAGCAGC AACTCCAACT CCCGGGGCAA ACACTTATGC ACCAAGCCCC CGCGCAGCAA
    TGGGGGAACC AGATGCAACA GAGAGCACCA ATTCCAGGGA CACTGATGAT GAGCGCGGGA
    CCTCGAGGCC CTGTCCCTCA GCAAGGCCTA CAGCAAGTGC AGGCTCAGAG TGTCATGGAA
    GACGACATCC TAATGGACCT TATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950711.1
    CDS273..2699
    Translation

    Target ORF information:

    RefSeq Version XM_018095222.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095222.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTC AGGCCGGTTG CGTACACTTC
    CCGCACACAA CTCCATGTGA AGTCCGAGTC CTCATGCTCC TCTACTCCTC CAAAAAGAAG
    ATCTTCATGG GGCTCATCCC TAACGACCAG AGTGGCTTCG TCAACGGTAT CCGACAGGTG
    ATCACAAACC ACAAGCAAGT TCAGATGCAG AAGATTGACC AACAGCGAAA TGTACGAATG
    ATGGGAGCTG GCCAAGCTGT GGGCACTGGG AATGTTCCAG CCAACACCCA AGCCTTCCTC
    CAGAAACAGC CAGGGGCTCT GCCAGTGGCA CAGGCCGTAC CCCAACAGAT GCAAGGCCAG
    CAGGTAGCCC CGGGCATGCC CTCCATTAGC CAAGTCACTA TGATGGATGA GCAACAGAGA
    ACACAGAATT TGTTACACAT CAGAGTACAG CAACCACAGC AAGCAGCCTC TCAGGCACCA
    CCACAAGCCA CACAAACCAC CGTTCAAGCA CCTGGCCAGC CTCAGAACCC ACAGCCAGGA
    GCGATGCTCC GACCACAAAA CCCTGGTGCC AACCCACAGC TACGCAACCT CCTCCTGAGC
    CAGCAGCCTC CACAGACCAG TGTCCCACAA ACACAGCAGC CTTTGCACCA TATGCAGCAA
    GCGGCTCAGG GCATGCTTCC CCATCAGGCC ATGGGCCAAC AGATGCAGCA CCAGGCTCCC
    GGCCAGCAGC AACTCCAACT CCCGGGGCAA ACACTTATGC ACCAAGCCCC CGCGCAGCAA
    TGGGGGAACC AGATGCAACA GAGAGCACCA ATTCCAGGGA CACTGATGAT GAGCGCGGGA
    CCTCGAGGCC CTGTCCCTCA GCAAGGCCTA CAGCAAGTGC AGGCTCAGAG TGTCATGGAA
    GACGACATCC TAATGGACCT TATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45298
    Clone ID Related Accession (Same CDS sequence) XM_018095222.1 , XM_018095221.1 , XM_018095223.1
    Accession Version XM_018095221.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTC AGGCCGGTTG CGTACACTTC
    CCGCACACAA CTCCATGTGA AGTCCGAGTC CTCATGCTCC TCTACTCCTC CAAAAAGAAG
    ATCTTCATGG GGCTCATCCC TAACGACCAG AGTGGCTTCG TCAACGGTAT CCGACAGGTG
    ATCACAAACC ACAAGCAAGT TCAGATGCAG AAGATTGACC AACAGCGAAA TGTACGAATG
    ATGGGAGCTG GCCAAGCTGT GGGCACTGGG AATGTTCCAG CCAACACCCA AGCCTTCCTC
    CAGAAACAGC CAGGGGCTCT GCCAGTGGCA CAGGCCGTAC CCCAACAGAT GCAAGGCCAG
    CAGGTAGCCC CGGGCATGCC CTCCATTAGC CAAGTCACTA TGATGGATGA GCAACAGAGA
    ACACAGAATT TGTTACACAT CAGAGTACAG CAACCACAGC AAGCAGCCTC TCAGGCACCA
    CCACAAGCCA CACAAACCAC CGTTCAAGCA CCTGGCCAGC CTCAGAACCC ACAGCCAGGA
    GCGATGCTCC GACCACAAAA CCCTGGTGCC AACCCACAGC TACGCAACCT CCTCCTGAGC
    CAGCAGCCTC CACAGACCAG TGTCCCACAA ACACAGCAGC CTTTGCACCA TATGCAGCAA
    GCGGCTCAGG GCATGCTTCC CCATCAGGCC ATGGGCCAAC AGATGCAGCA CCAGGCTCCC
    GGCCAGCAGC AACTCCAACT CCCGGGGCAA ACACTTATGC ACCAAGCCCC CGCGCAGCAA
    TGGGGGAACC AGATGCAACA GAGAGCACCA ATTCCAGGGA CACTGATGAT GAGCGCGGGA
    CCTCGAGGCC CTGTCCCTCA GCAAGGCCTA CAGCAAGTGC AGGCTCAGAG TGTCATGGAA
    GACGACATCC TAATGGACCT TATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950710.1
    CDS190..2616
    Translation

    Target ORF information:

    RefSeq Version XM_018095221.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095221.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTC AGGCCGGTTG CGTACACTTC
    CCGCACACAA CTCCATGTGA AGTCCGAGTC CTCATGCTCC TCTACTCCTC CAAAAAGAAG
    ATCTTCATGG GGCTCATCCC TAACGACCAG AGTGGCTTCG TCAACGGTAT CCGACAGGTG
    ATCACAAACC ACAAGCAAGT TCAGATGCAG AAGATTGACC AACAGCGAAA TGTACGAATG
    ATGGGAGCTG GCCAAGCTGT GGGCACTGGG AATGTTCCAG CCAACACCCA AGCCTTCCTC
    CAGAAACAGC CAGGGGCTCT GCCAGTGGCA CAGGCCGTAC CCCAACAGAT GCAAGGCCAG
    CAGGTAGCCC CGGGCATGCC CTCCATTAGC CAAGTCACTA TGATGGATGA GCAACAGAGA
    ACACAGAATT TGTTACACAT CAGAGTACAG CAACCACAGC AAGCAGCCTC TCAGGCACCA
    CCACAAGCCA CACAAACCAC CGTTCAAGCA CCTGGCCAGC CTCAGAACCC ACAGCCAGGA
    GCGATGCTCC GACCACAAAA CCCTGGTGCC AACCCACAGC TACGCAACCT CCTCCTGAGC
    CAGCAGCCTC CACAGACCAG TGTCCCACAA ACACAGCAGC CTTTGCACCA TATGCAGCAA
    GCGGCTCAGG GCATGCTTCC CCATCAGGCC ATGGGCCAAC AGATGCAGCA CCAGGCTCCC
    GGCCAGCAGC AACTCCAACT CCCGGGGCAA ACACTTATGC ACCAAGCCCC CGCGCAGCAA
    TGGGGGAACC AGATGCAACA GAGAGCACCA ATTCCAGGGA CACTGATGAT GAGCGCGGGA
    CCTCGAGGCC CTGTCCCTCA GCAAGGCCTA CAGCAAGTGC AGGCTCAGAG TGTCATGGAA
    GACGACATCC TAATGGACCT TATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45298
    Clone ID Related Accession (Same CDS sequence) XM_018095222.1 , XM_018095221.1 , XM_018095223.1
    Accession Version XM_018095223.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTC AGGCCGGTTG CGTACACTTC
    CCGCACACAA CTCCATGTGA AGTCCGAGTC CTCATGCTCC TCTACTCCTC CAAAAAGAAG
    ATCTTCATGG GGCTCATCCC TAACGACCAG AGTGGCTTCG TCAACGGTAT CCGACAGGTG
    ATCACAAACC ACAAGCAAGT TCAGATGCAG AAGATTGACC AACAGCGAAA TGTACGAATG
    ATGGGAGCTG GCCAAGCTGT GGGCACTGGG AATGTTCCAG CCAACACCCA AGCCTTCCTC
    CAGAAACAGC CAGGGGCTCT GCCAGTGGCA CAGGCCGTAC CCCAACAGAT GCAAGGCCAG
    CAGGTAGCCC CGGGCATGCC CTCCATTAGC CAAGTCACTA TGATGGATGA GCAACAGAGA
    ACACAGAATT TGTTACACAT CAGAGTACAG CAACCACAGC AAGCAGCCTC TCAGGCACCA
    CCACAAGCCA CACAAACCAC CGTTCAAGCA CCTGGCCAGC CTCAGAACCC ACAGCCAGGA
    GCGATGCTCC GACCACAAAA CCCTGGTGCC AACCCACAGC TACGCAACCT CCTCCTGAGC
    CAGCAGCCTC CACAGACCAG TGTCCCACAA ACACAGCAGC CTTTGCACCA TATGCAGCAA
    GCGGCTCAGG GCATGCTTCC CCATCAGGCC ATGGGCCAAC AGATGCAGCA CCAGGCTCCC
    GGCCAGCAGC AACTCCAACT CCCGGGGCAA ACACTTATGC ACCAAGCCCC CGCGCAGCAA
    TGGGGGAACC AGATGCAACA GAGAGCACCA ATTCCAGGGA CACTGATGAT GAGCGCGGGA
    CCTCGAGGCC CTGTCCCTCA GCAAGGCCTA CAGCAAGTGC AGGCTCAGAG TGTCATGGAA
    GACGACATCC TAATGGACCT TATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950712.1
    CDS270..2696
    Translation

    Target ORF information:

    RefSeq Version XM_018095223.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095223.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTC AGGCCGGTTG CGTACACTTC
    CCGCACACAA CTCCATGTGA AGTCCGAGTC CTCATGCTCC TCTACTCCTC CAAAAAGAAG
    ATCTTCATGG GGCTCATCCC TAACGACCAG AGTGGCTTCG TCAACGGTAT CCGACAGGTG
    ATCACAAACC ACAAGCAAGT TCAGATGCAG AAGATTGACC AACAGCGAAA TGTACGAATG
    ATGGGAGCTG GCCAAGCTGT GGGCACTGGG AATGTTCCAG CCAACACCCA AGCCTTCCTC
    CAGAAACAGC CAGGGGCTCT GCCAGTGGCA CAGGCCGTAC CCCAACAGAT GCAAGGCCAG
    CAGGTAGCCC CGGGCATGCC CTCCATTAGC CAAGTCACTA TGATGGATGA GCAACAGAGA
    ACACAGAATT TGTTACACAT CAGAGTACAG CAACCACAGC AAGCAGCCTC TCAGGCACCA
    CCACAAGCCA CACAAACCAC CGTTCAAGCA CCTGGCCAGC CTCAGAACCC ACAGCCAGGA
    GCGATGCTCC GACCACAAAA CCCTGGTGCC AACCCACAGC TACGCAACCT CCTCCTGAGC
    CAGCAGCCTC CACAGACCAG TGTCCCACAA ACACAGCAGC CTTTGCACCA TATGCAGCAA
    GCGGCTCAGG GCATGCTTCC CCATCAGGCC ATGGGCCAAC AGATGCAGCA CCAGGCTCCC
    GGCCAGCAGC AACTCCAACT CCCGGGGCAA ACACTTATGC ACCAAGCCCC CGCGCAGCAA
    TGGGGGAACC AGATGCAACA GAGAGCACCA ATTCCAGGGA CACTGATGAT GAGCGCGGGA
    CCTCGAGGCC CTGTCCCTCA GCAAGGCCTA CAGCAAGTGC AGGCTCAGAG TGTCATGGAA
    GACGACATCC TAATGGACCT TATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45299
    Clone ID Related Accession (Same CDS sequence) XM_018095224.1
    Accession Version XM_018095224.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2424bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTG CCGGTTGCGT ACACTTCCCG
    CACACAACTC CATGTGAAGT CCGAGTCCTC ATGCTCCTCT ACTCCTCCAA AAAGAAGATC
    TTCATGGGGC TCATCCCTAA CGACCAGAGT GGCTTCGTCA ACGGTATCCG ACAGGTGATC
    ACAAACCACA AGCAAGTTCA GATGCAGAAG ATTGACCAAC AGCGAAATGT ACGAATGATG
    GGAGCTGGCC AAGCTGTGGG CACTGGGAAT GTTCCAGCCA ACACCCAAGC CTTCCTCCAG
    AAACAGCCAG GGGCTCTGCC AGTGGCACAG GCCGTACCCC AACAGATGCA AGGCCAGCAG
    GTAGCCCCGG GCATGCCCTC CATTAGCCAA GTCACTATGA TGGATGAGCA ACAGAGAACA
    CAGAATTTGT TACACATCAG AGTACAGCAA CCACAGCAAG CAGCCTCTCA GGCACCACCA
    CAAGCCACAC AAACCACCGT TCAAGCACCT GGCCAGCCTC AGAACCCACA GCCAGGAGCG
    ATGCTCCGAC CACAAAACCC TGGTGCCAAC CCACAGCTAC GCAACCTCCT CCTGAGCCAG
    CAGCCTCCAC AGACCAGTGT CCCACAAACA CAGCAGCCTT TGCACCATAT GCAGCAAGCG
    GCTCAGGGCA TGCTTCCCCA TCAGGCCATG GGCCAACAGA TGCAGCACCA GGCTCCCGGC
    CAGCAGCAAC TCCAACTCCC GGGGCAAACA CTTATGCACC AAGCCCCCGC GCAGCAATGG
    GGGAACCAGA TGCAACAGAG AGCACCAATT CCAGGGACAC TGATGATGAG CGCGGGACCT
    CGAGGCCCTG TCCCTCAGCA AGGCCTACAG CAAGTGCAGG CTCAGAGTGT CATGGAAGAC
    GACATCCTAA TGGACCTTAT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950713.1
    CDS189..2612
    Translation

    Target ORF information:

    RefSeq Version XM_018095224.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095224.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGAAG CCTCGGTCAG TGTCAGTGGA CAACAACGCC
    AAACTAACTC GCTCCCTTCC ATGTCAAGTT TATGTCAACC CTGGAGAAAA TCTAAAAACT
    GATCAATGGC CACAAAAGCT TATTATGCAG CTCATCCCAC AGCAACTTCT GACTACACTT
    GGACCTTTGT TTAGGAATTC TCGCATGGTT CAGTTCCACT TTACCAACAA GGACCTAGAA
    TCTCTGAAAG GCCTGTACCG TATCATGGGC AGTGGCTTTG CCGGTTGCGT ACACTTCCCG
    CACACAACTC CATGTGAAGT CCGAGTCCTC ATGCTCCTCT ACTCCTCCAA AAAGAAGATC
    TTCATGGGGC TCATCCCTAA CGACCAGAGT GGCTTCGTCA ACGGTATCCG ACAGGTGATC
    ACAAACCACA AGCAAGTTCA GATGCAGAAG ATTGACCAAC AGCGAAATGT ACGAATGATG
    GGAGCTGGCC AAGCTGTGGG CACTGGGAAT GTTCCAGCCA ACACCCAAGC CTTCCTCCAG
    AAACAGCCAG GGGCTCTGCC AGTGGCACAG GCCGTACCCC AACAGATGCA AGGCCAGCAG
    GTAGCCCCGG GCATGCCCTC CATTAGCCAA GTCACTATGA TGGATGAGCA ACAGAGAACA
    CAGAATTTGT TACACATCAG AGTACAGCAA CCACAGCAAG CAGCCTCTCA GGCACCACCA
    CAAGCCACAC AAACCACCGT TCAAGCACCT GGCCAGCCTC AGAACCCACA GCCAGGAGCG
    ATGCTCCGAC CACAAAACCC TGGTGCCAAC CCACAGCTAC GCAACCTCCT CCTGAGCCAG
    CAGCCTCCAC AGACCAGTGT CCCACAAACA CAGCAGCCTT TGCACCATAT GCAGCAAGCG
    GCTCAGGGCA TGCTTCCCCA TCAGGCCATG GGCCAACAGA TGCAGCACCA GGCTCCCGGC
    CAGCAGCAAC TCCAACTCCC GGGGCAAACA CTTATGCACC AAGCCCCCGC GCAGCAATGG
    GGGAACCAGA TGCAACAGAG AGCACCAATT CCAGGGACAC TGATGATGAG CGCGGGACCT
    CGAGGCCCTG TCCCTCAGCA AGGCCTACAG CAAGTGCAGG CTCAGAGTGT CATGGAAGAC
    GACATCCTAA TGGACCTTAT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45300
    Clone ID Related Accession (Same CDS sequence) XM_018095225.1
    Accession Version XM_018095225.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2424bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGGTT CCTCAGCAGA ATACTTTGAC TACAGCACAT
    GCTGCTGCCC AGTCTGCGGT TGAAGCTGCA AAGAATCAGA AAAATAACCT CCCAAATCGC
    TTTCCACTTC CAAACCTTAA TCAGATGCCA CCTACATCGG CTGTTGGTAC TCCATTTAAC
    CAACCGCCGC CCCCTGCAAT GCCTCAGAAT ACCTCAGTCC CCAAGATGGT TACTTCCACT
    GCATCCTTAA TGACGCCTGC TTCTCAACCC AGTCTTGTCA CCACGGTAAC CACAGGTCCT
    GGACCAGCGC CTGTACAATT GCAGCAGCAG GGAGCTCAGC AACAGCCTGT TCCGCCATCT
    ATGCCCATAA CAGGAGCTGC TGGGGGAGTT CAAGCCCCAC AACCCTCACA GCCACAGATT
    GGAACAGCAC AGTTGCCATG CACACAGACA CCTGTTAATG GCAATAAATT GCTTGCCTGG
    AGTGGGGTGC TCGAGTGGCA AGAGAAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTCAGG CCGGTTGCGT ACACTTCCCG
    CACACAACTC CATGTGAAGT CCGAGTCCTC ATGCTCCTCT ACTCCTCCAA AAAGAAGATC
    TTCATGGGGC TCATCCCTAA CGACCAGAGT GGCTTCGTCA ACGGTATCCG ACAGGTGATC
    ACAAACCACA AGCAAGTTCA GATGCAGAAG ATTGACCAAC AGCGAAATGT ACGAATGATG
    GGAGCTGGCC AAGCTGTGGG CACTGGGAAT GTTCCAGCCA ACACCCAAGC CTTCCTCCAG
    AAACAGCCAG GGGCTCTGCC AGTGGCACAG GCCGTACCCC AACAGATGCA AGGCCAGCAG
    GTAGCCCCGG GCATGCCCTC CATTAGCCAA GTCACTATGA TGGATGAGCA ACAGAGAACA
    CAGAATTTGT TACACATCAG AGTACAGCAA CCACAGCAAG CAGCCTCTCA GGCACCACCA
    CAAGCCACAC AAACCACCGT TCAAGCACCT GGCCAGCCTC AGAACCCACA GCCAGGAGCG
    ATGCTCCGAC CACAAAACCC TGGTGCCAAC CCACAGCTAC GCAACCTCCT CCTGAGCCAG
    CAGCCTCCAC AGACCAGTGT CCCACAAACA CAGCAGCCTT TGCACCATAT GCAGCAAGCG
    GCTCAGGGCA TGCTTCCCCA TCAGGCCATG GGCCAACAGA TGCAGCACCA GGCTCCCGGC
    CAGCAGCAAC TCCAACTCCC GGGGCAAACA CTTATGCACC AAGCCCCCGC GCAGCAATGG
    GGGAACCAGA TGCAACAGAG AGCACCAATT CCAGGGACAC TGATGATGAG CGCGGGACCT
    CGAGGCCCTG TCCCTCAGCA AGGCCTACAG CAAGTGCAGG CTCAGAGTGT CATGGAAGAC
    GACATCCTAA TGGACCTTAT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950714.1
    CDS190..2613
    Translation

    Target ORF information:

    RefSeq Version XM_018095225.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095225.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGGTT CCTCAGCAGA ATACTTTGAC TACAGCACAT
    GCTGCTGCCC AGTCTGCGGT TGAAGCTGCA AAGAATCAGA AAAATAACCT CCCAAATCGC
    TTTCCACTTC CAAACCTTAA TCAGATGCCA CCTACATCGG CTGTTGGTAC TCCATTTAAC
    CAACCGCCGC CCCCTGCAAT GCCTCAGAAT ACCTCAGTCC CCAAGATGGT TACTTCCACT
    GCATCCTTAA TGACGCCTGC TTCTCAACCC AGTCTTGTCA CCACGGTAAC CACAGGTCCT
    GGACCAGCGC CTGTACAATT GCAGCAGCAG GGAGCTCAGC AACAGCCTGT TCCGCCATCT
    ATGCCCATAA CAGGAGCTGC TGGGGGAGTT CAAGCCCCAC AACCCTCACA GCCACAGATT
    GGAACAGCAC AGTTGCCATG CACACAGACA CCTGTTAATG GCAATAAATT GCTTGCCTGG
    AGTGGGGTGC TCGAGTGGCA AGAGAAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTCAGG CCGGTTGCGT ACACTTCCCG
    CACACAACTC CATGTGAAGT CCGAGTCCTC ATGCTCCTCT ACTCCTCCAA AAAGAAGATC
    TTCATGGGGC TCATCCCTAA CGACCAGAGT GGCTTCGTCA ACGGTATCCG ACAGGTGATC
    ACAAACCACA AGCAAGTTCA GATGCAGAAG ATTGACCAAC AGCGAAATGT ACGAATGATG
    GGAGCTGGCC AAGCTGTGGG CACTGGGAAT GTTCCAGCCA ACACCCAAGC CTTCCTCCAG
    AAACAGCCAG GGGCTCTGCC AGTGGCACAG GCCGTACCCC AACAGATGCA AGGCCAGCAG
    GTAGCCCCGG GCATGCCCTC CATTAGCCAA GTCACTATGA TGGATGAGCA ACAGAGAACA
    CAGAATTTGT TACACATCAG AGTACAGCAA CCACAGCAAG CAGCCTCTCA GGCACCACCA
    CAAGCCACAC AAACCACCGT TCAAGCACCT GGCCAGCCTC AGAACCCACA GCCAGGAGCG
    ATGCTCCGAC CACAAAACCC TGGTGCCAAC CCACAGCTAC GCAACCTCCT CCTGAGCCAG
    CAGCCTCCAC AGACCAGTGT CCCACAAACA CAGCAGCCTT TGCACCATAT GCAGCAAGCG
    GCTCAGGGCA TGCTTCCCCA TCAGGCCATG GGCCAACAGA TGCAGCACCA GGCTCCCGGC
    CAGCAGCAAC TCCAACTCCC GGGGCAAACA CTTATGCACC AAGCCCCCGC GCAGCAATGG
    GGGAACCAGA TGCAACAGAG AGCACCAATT CCAGGGACAC TGATGATGAG CGCGGGACCT
    CGAGGCCCTG TCCCTCAGCA AGGCCTACAG CAAGTGCAGG CTCAGAGTGT CATGGAAGAC
    GACATCCTAA TGGACCTTAT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45301
    Clone ID Related Accession (Same CDS sequence) XM_018095226.1
    Accession Version XM_018095226.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2424bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTCAGG CCGGTTGCGT ACACTTCCCG
    CACACAACTC CATGTGAAGT CCGAGTCCTC ATGCTCCTCT ACTCCTCCAA AAAGAAGATC
    TTCATGGGGC TCATCCCTAA CGACCAGAGT GGCTTCGTCA ACGGTATCCG ACAGGTGATC
    ACAAACCACA AGCAAGTTCA GATGCAGAAG ATTGACCAAC AGCGAAATGT ACGAATGATG
    GGAGCTGGCC AAGCTGTGGG CACTGGGAAT GTTCCAGCCA ACACCCAAGC CTTCCTCCAG
    AAACAGCCAG GGGCTCTGCC AGTGGCACAG GCCGTACCCC AACAGATGCA AGGCCAGCAG
    GTAGCCCCGG GCATGCCCTC CATTAGCCAA GTCACTATGA TGGATGAGCA ACAGAGAACA
    CAGAATTTGT TACACATCAG AGTACAGCAA CCACAGCAAG CAGCCTCTCA GGCACCACCA
    CAAGCCACAC AAACCACCGT TCAAGCACCT GGCCAGCCTC AGAACCCACA GCCAGGAGCG
    ATGCTCCGAC CACAAAACCC TGGTGCCAAC CCACAGCTAC GCAACCTCCT CCTGAGCCAG
    CAGCCTCCAC AGACCAGTGT CCCACAAACA CAGCAGCCTT TGCACCATAT GCAGCAAGCG
    GCTCAGGGCA TGCTTCCCCA TCAGGCCATG GGCCAACAGA TGCAGCACCA GGCTCCCGGC
    CAGCAGCAAC TCCAACTCCC GGGGCAAACA CTTATGCACC AAGCCCCCGC GCAGCAATGG
    GGGAACCAGA TGCAACAGAG AGCACCAATT CCAGGGACAC TGATGATGAG CGCGGGACCT
    CGAGGCCCTG TCCCTCAGCA AGGCCTACAG CAAGTGCAGG CTCAGAGTGT CATGGAAGAC
    GACATCCTAA TGGACCTTAT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950715.1
    CDS190..2613
    Translation

    Target ORF information:

    RefSeq Version XM_018095226.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095226.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTCAGG CCGGTTGCGT ACACTTCCCG
    CACACAACTC CATGTGAAGT CCGAGTCCTC ATGCTCCTCT ACTCCTCCAA AAAGAAGATC
    TTCATGGGGC TCATCCCTAA CGACCAGAGT GGCTTCGTCA ACGGTATCCG ACAGGTGATC
    ACAAACCACA AGCAAGTTCA GATGCAGAAG ATTGACCAAC AGCGAAATGT ACGAATGATG
    GGAGCTGGCC AAGCTGTGGG CACTGGGAAT GTTCCAGCCA ACACCCAAGC CTTCCTCCAG
    AAACAGCCAG GGGCTCTGCC AGTGGCACAG GCCGTACCCC AACAGATGCA AGGCCAGCAG
    GTAGCCCCGG GCATGCCCTC CATTAGCCAA GTCACTATGA TGGATGAGCA ACAGAGAACA
    CAGAATTTGT TACACATCAG AGTACAGCAA CCACAGCAAG CAGCCTCTCA GGCACCACCA
    CAAGCCACAC AAACCACCGT TCAAGCACCT GGCCAGCCTC AGAACCCACA GCCAGGAGCG
    ATGCTCCGAC CACAAAACCC TGGTGCCAAC CCACAGCTAC GCAACCTCCT CCTGAGCCAG
    CAGCCTCCAC AGACCAGTGT CCCACAAACA CAGCAGCCTT TGCACCATAT GCAGCAAGCG
    GCTCAGGGCA TGCTTCCCCA TCAGGCCATG GGCCAACAGA TGCAGCACCA GGCTCCCGGC
    CAGCAGCAAC TCCAACTCCC GGGGCAAACA CTTATGCACC AAGCCCCCGC GCAGCAATGG
    GGGAACCAGA TGCAACAGAG AGCACCAATT CCAGGGACAC TGATGATGAG CGCGGGACCT
    CGAGGCCCTG TCCCTCAGCA AGGCCTACAG CAAGTGCAGG CTCAGAGTGT CATGGAAGAC
    GACATCCTAA TGGACCTTAT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45302
    Clone ID Related Accession (Same CDS sequence) XM_018095227.1
    Accession Version XM_018095227.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2421bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGGTT CCTCAGCAGA ATACTTTGAC TACAGCACAT
    GCTGCTGCCC AGTCTGCGGT TGAAGCTGCA AAGAATCAGA AAAATAACCT CCCAAATCGC
    TTTCCACTTC CAAACCTTAA TCAGATGCCA CCTACATCGG CTGTTGGTAC TCCATTTAAC
    CAACCGCCGC CCCCTGCAAT GCCTCAGAAT ACCTCAGTCC CCAAGATGGT TACTTCCACT
    GCATCCTTAA TGACGCCTGC TTCTCAACCC AGTCTTGTCA CCACGGTAAC CACAGGTCCT
    GGACCAGCGC CTGTACAATT GCAGCAGCAG GGAGCTCAGC AACAGCCTGT TCCGCCATCT
    ATGCCCATAA CAGGAGCTGC TGGGGGAGTT CAAGCCCCAC AACCCTCACA GCCACAGATT
    GGAACAGCAC AGTTGCCATG CACACAGACA CCTGTTAATG GCAATAAATT GCTTGCCTGG
    AGTGGGGTGC TCGAGTGGCA AGAGAAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTGCCG GTTGCGTACA CTTCCCGCAC
    ACAACTCCAT GTGAAGTCCG AGTCCTCATG CTCCTCTACT CCTCCAAAAA GAAGATCTTC
    ATGGGGCTCA TCCCTAACGA CCAGAGTGGC TTCGTCAACG GTATCCGACA GGTGATCACA
    AACCACAAGC AAGTTCAGAT GCAGAAGATT GACCAACAGC GAAATGTACG AATGATGGGA
    GCTGGCCAAG CTGTGGGCAC TGGGAATGTT CCAGCCAACA CCCAAGCCTT CCTCCAGAAA
    CAGCCAGGGG CTCTGCCAGT GGCACAGGCC GTACCCCAAC AGATGCAAGG CCAGCAGGTA
    GCCCCGGGCA TGCCCTCCAT TAGCCAAGTC ACTATGATGG ATGAGCAACA GAGAACACAG
    AATTTGTTAC ACATCAGAGT ACAGCAACCA CAGCAAGCAG CCTCTCAGGC ACCACCACAA
    GCCACACAAA CCACCGTTCA AGCACCTGGC CAGCCTCAGA ACCCACAGCC AGGAGCGATG
    CTCCGACCAC AAAACCCTGG TGCCAACCCA CAGCTACGCA ACCTCCTCCT GAGCCAGCAG
    CCTCCACAGA CCAGTGTCCC ACAAACACAG CAGCCTTTGC ACCATATGCA GCAAGCGGCT
    CAGGGCATGC TTCCCCATCA GGCCATGGGC CAACAGATGC AGCACCAGGC TCCCGGCCAG
    CAGCAACTCC AACTCCCGGG GCAAACACTT ATGCACCAAG CCCCCGCGCA GCAATGGGGG
    AACCAGATGC AACAGAGAGC ACCAATTCCA GGGACACTGA TGATGAGCGC GGGACCTCGA
    GGCCCTGTCC CTCAGCAAGG CCTACAGCAA GTGCAGGCTC AGAGTGTCAT GGAAGACGAC
    ATCCTAATGG ACCTTATTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950716.1
    CDS189..2609
    Translation

    Target ORF information:

    RefSeq Version XM_018095227.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095227.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGGTT CCTCAGCAGA ATACTTTGAC TACAGCACAT
    GCTGCTGCCC AGTCTGCGGT TGAAGCTGCA AAGAATCAGA AAAATAACCT CCCAAATCGC
    TTTCCACTTC CAAACCTTAA TCAGATGCCA CCTACATCGG CTGTTGGTAC TCCATTTAAC
    CAACCGCCGC CCCCTGCAAT GCCTCAGAAT ACCTCAGTCC CCAAGATGGT TACTTCCACT
    GCATCCTTAA TGACGCCTGC TTCTCAACCC AGTCTTGTCA CCACGGTAAC CACAGGTCCT
    GGACCAGCGC CTGTACAATT GCAGCAGCAG GGAGCTCAGC AACAGCCTGT TCCGCCATCT
    ATGCCCATAA CAGGAGCTGC TGGGGGAGTT CAAGCCCCAC AACCCTCACA GCCACAGATT
    GGAACAGCAC AGTTGCCATG CACACAGACA CCTGTTAATG GCAATAAATT GCTTGCCTGG
    AGTGGGGTGC TCGAGTGGCA AGAGAAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTGCCG GTTGCGTACA CTTCCCGCAC
    ACAACTCCAT GTGAAGTCCG AGTCCTCATG CTCCTCTACT CCTCCAAAAA GAAGATCTTC
    ATGGGGCTCA TCCCTAACGA CCAGAGTGGC TTCGTCAACG GTATCCGACA GGTGATCACA
    AACCACAAGC AAGTTCAGAT GCAGAAGATT GACCAACAGC GAAATGTACG AATGATGGGA
    GCTGGCCAAG CTGTGGGCAC TGGGAATGTT CCAGCCAACA CCCAAGCCTT CCTCCAGAAA
    CAGCCAGGGG CTCTGCCAGT GGCACAGGCC GTACCCCAAC AGATGCAAGG CCAGCAGGTA
    GCCCCGGGCA TGCCCTCCAT TAGCCAAGTC ACTATGATGG ATGAGCAACA GAGAACACAG
    AATTTGTTAC ACATCAGAGT ACAGCAACCA CAGCAAGCAG CCTCTCAGGC ACCACCACAA
    GCCACACAAA CCACCGTTCA AGCACCTGGC CAGCCTCAGA ACCCACAGCC AGGAGCGATG
    CTCCGACCAC AAAACCCTGG TGCCAACCCA CAGCTACGCA ACCTCCTCCT GAGCCAGCAG
    CCTCCACAGA CCAGTGTCCC ACAAACACAG CAGCCTTTGC ACCATATGCA GCAAGCGGCT
    CAGGGCATGC TTCCCCATCA GGCCATGGGC CAACAGATGC AGCACCAGGC TCCCGGCCAG
    CAGCAACTCC AACTCCCGGG GCAAACACTT ATGCACCAAG CCCCCGCGCA GCAATGGGGG
    AACCAGATGC AACAGAGAGC ACCAATTCCA GGGACACTGA TGATGAGCGC GGGACCTCGA
    GGCCCTGTCC CTCAGCAAGG CCTACAGCAA GTGCAGGCTC AGAGTGTCAT GGAAGACGAC
    ATCCTAATGG ACCTTATTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45303
    Clone ID Related Accession (Same CDS sequence) XM_018095228.1
    Accession Version XM_018095228.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2421bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mediator of RNA polymerase II transcription subunit 25 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTGCCG GTTGCGTACA CTTCCCGCAC
    ACAACTCCAT GTGAAGTCCG AGTCCTCATG CTCCTCTACT CCTCCAAAAA GAAGATCTTC
    ATGGGGCTCA TCCCTAACGA CCAGAGTGGC TTCGTCAACG GTATCCGACA GGTGATCACA
    AACCACAAGC AAGTTCAGAT GCAGAAGATT GACCAACAGC GAAATGTACG AATGATGGGA
    GCTGGCCAAG CTGTGGGCAC TGGGAATGTT CCAGCCAACA CCCAAGCCTT CCTCCAGAAA
    CAGCCAGGGG CTCTGCCAGT GGCACAGGCC GTACCCCAAC AGATGCAAGG CCAGCAGGTA
    GCCCCGGGCA TGCCCTCCAT TAGCCAAGTC ACTATGATGG ATGAGCAACA GAGAACACAG
    AATTTGTTAC ACATCAGAGT ACAGCAACCA CAGCAAGCAG CCTCTCAGGC ACCACCACAA
    GCCACACAAA CCACCGTTCA AGCACCTGGC CAGCCTCAGA ACCCACAGCC AGGAGCGATG
    CTCCGACCAC AAAACCCTGG TGCCAACCCA CAGCTACGCA ACCTCCTCCT GAGCCAGCAG
    CCTCCACAGA CCAGTGTCCC ACAAACACAG CAGCCTTTGC ACCATATGCA GCAAGCGGCT
    CAGGGCATGC TTCCCCATCA GGCCATGGGC CAACAGATGC AGCACCAGGC TCCCGGCCAG
    CAGCAACTCC AACTCCCGGG GCAAACACTT ATGCACCAAG CCCCCGCGCA GCAATGGGGG
    AACCAGATGC AACAGAGAGC ACCAATTCCA GGGACACTGA TGATGAGCGC GGGACCTCGA
    GGCCCTGTCC CTCAGCAAGG CCTACAGCAA GTGCAGGCTC AGAGTGTCAT GGAAGACGAC
    ATCCTAATGG ACCTTATTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950717.1
    CDS190..2610
    Translation

    Target ORF information:

    RefSeq Version XM_018095228.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mediator complex subunit 25 (med25), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095228.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATGCAG CCACACCAGG AAACGGCGGC ATAATATCCG ATGTTGTCTT TGTCATAGAG 
    GGAACGGCTA ATTTAGGACC TTACTTTGAA TCACTACGGA AACATTACCT GTTACCTGCA
    ATAGAGTATT TTAACGGTGG ACCCCCAGCA GAGACTGACT TTGGAGGAGA TTATGGCGGC
    ACCCAATACA GCCTGGTAGT GTTTAATACG GTGGACTGTG CTCCAGAATC CTATGTCCAA
    TGTCATGCCC CCACCAGCAG TGCTTATGAA TTTGTCCAAT GGCTGGACAG CATTAGGTTC
    ATGGGAGGCG GAGGTGAGAG CTGTAGTTTG ATTGCTGAGG GGCTAAGCAC TGCCCTACAA
    CTTTTTGATG ACTTCAAAAA GATGAGGGAA CAGATTGGGC AAACTCACAA GGTGTGCATC
    TTGATTTGCA ACTCCCCTCC ATATCTACTG CCTGCTGTAG AGAGCACAAC CTACTCTGGA
    TACACAACTG AGAACCTTGT ACAGAAAATT GGGGAGAGAG GAATCCATTT CTCCGTCATT
    TCCCCGCGCA AACTGCCAGC ATTACGAACT CTGTTTGAGA AAGCCATGCC AGTGGGTTTG
    ATCGAGCCAC AGCCTAAAGA CTACAGCCAG GACCCTCGGC ACATGATACT TGTACGCGGA
    ATGGTTCTTC CAGTAGGAGG AGCTACATCT GTTCCAGGAG TCATTCCACC TAAACAACCT
    ATTTCACAGC CACCACTTCC AGTTGTCCCA CCCCAGATTG CAAATGCCCC TTCGCATCAG
    CTCCCACCTG TCCAGCCACC TTATATGCAG GTTCCTCAGC AGAATACTTT GACTACAGCA
    CATGCTGCTG CCCAGTCTGC GGTTGAAGCT GCAAAGAATC AGAAAAATAA CCTCCCAAAT
    CGCTTTCCAC TTCCAAACCT TAATCAGATG CCACCTACAT CGGCTGTTGG TACTCCATTT
    AACCAACCGC CGCCCCCTGC AATGCCTCAG AATACCTCAG TCCCCAAGAT GGTTACTTCC
    ACTGCATCCT TAATGACGCC TGCTTCTCAA CCCAGTCTTG TCACCACGGT AACCACAGGT
    CCTGGACCAG CGCCTGTACA ATTGCAGCAG CAGGGAGCTC AGCAACAGCC TGTTCCGCCA
    TCTATGCCCA TAACAGGAGC TGCTGGGGGA GTTCAAGCCC CACAACCCTC ACAGCCACAG
    ATTGGAACAG CACAGTTGCC ATGCACACAG ACACCTGTTA ATGGCAATAA ATTGCTTGCC
    TGGAGTGGGG TGCTCGAGTG GCAAGAGCCT CGGTCAGTGT CAGTGGACAA CAACGCCAAA
    CTAACTCGCT CCCTTCCATG TCAAGTTTAT GTCAACCCTG GAGAAAATCT AAAAACTGAT
    CAATGGCCAC AAAAGCTTAT TATGCAGCTC ATCCCACAGC AACTTCTGAC TACACTTGGA
    CCTTTGTTTA GGAATTCTCG CATGGTTCAG TTCCACTTTA CCAACAAGGA CCTAGAATCT
    CTGAAAGGCC TGTACCGTAT CATGGGCAGT GGCTTTGCCG GTTGCGTACA CTTCCCGCAC
    ACAACTCCAT GTGAAGTCCG AGTCCTCATG CTCCTCTACT CCTCCAAAAA GAAGATCTTC
    ATGGGGCTCA TCCCTAACGA CCAGAGTGGC TTCGTCAACG GTATCCGACA GGTGATCACA
    AACCACAAGC AAGTTCAGAT GCAGAAGATT GACCAACAGC GAAATGTACG AATGATGGGA
    GCTGGCCAAG CTGTGGGCAC TGGGAATGTT CCAGCCAACA CCCAAGCCTT CCTCCAGAAA
    CAGCCAGGGG CTCTGCCAGT GGCACAGGCC GTACCCCAAC AGATGCAAGG CCAGCAGGTA
    GCCCCGGGCA TGCCCTCCAT TAGCCAAGTC ACTATGATGG ATGAGCAACA GAGAACACAG
    AATTTGTTAC ACATCAGAGT ACAGCAACCA CAGCAAGCAG CCTCTCAGGC ACCACCACAA
    GCCACACAAA CCACCGTTCA AGCACCTGGC CAGCCTCAGA ACCCACAGCC AGGAGCGATG
    CTCCGACCAC AAAACCCTGG TGCCAACCCA CAGCTACGCA ACCTCCTCCT GAGCCAGCAG
    CCTCCACAGA CCAGTGTCCC ACAAACACAG CAGCCTTTGC ACCATATGCA GCAAGCGGCT
    CAGGGCATGC TTCCCCATCA GGCCATGGGC CAACAGATGC AGCACCAGGC TCCCGGCCAG
    CAGCAACTCC AACTCCCGGG GCAAACACTT ATGCACCAAG CCCCCGCGCA GCAATGGGGG
    AACCAGATGC AACAGAGAGC ACCAATTCCA GGGACACTGA TGATGAGCGC GGGACCTCGA
    GGCCCTGTCC CTCAGCAAGG CCTACAGCAA GTGCAGGCTC AGAGTGTCAT GGAAGACGAC
    ATCCTAATGG ACCTTATTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.