TMEM204 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEM204
Entrez Gene ID	79652
Full Name	transmembrane protein 204
Synonyms	C16orf30,CLP24
General protein information	Preferred Names transmembrane protein 204 Names transmembrane protein 204 claudin-like protein 24 claudin-like protein of 24 kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	C16ORF30 plays a role in cell adhesion and cellular permeability at adherens junctions (Kearsey et al., 2004 [PubMed 15206924]).[supplied by OMIM, Mar 2008].
Disorder MIM:	611002

mRNA and Protein(s)

mRNA	Protein	Name
NM_024600.5	NP_078876.2	transmembrane protein 204 precursor
NM_024600.6	NP_078876.2	transmembrane protein 204 precursor
NM_001256541.2	NP_001243470.1	transmembrane protein 204 precursor
NM_001256541.1	NP_001243470.1	transmembrane protein 204 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tmem204	NP_001009620.1
Homo sapiens (human)	TMEM204	NP_078876.2
	TMEM204	XP_001089509.1
Bos taurus (cattle)	TMEM204	NP_001069845.1
Gallus gallus (chicken)	TMEM204	XP_415054.1
Canis lupus familiaris (dog)	TMEM204	XP_003434919.1
Mus musculus (house mouse)	Tmem204	NP_001001183.1
Danio rerio (zebrafish)	tmem204	NP_001157369.1
Xenopus tropicalis (tropical clawed frog)	LOC100135192	NP_001107367.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel four transmembrane spanning protein, CLP24. A hypoxically regulated cell junction protein.
Kearsey J, Petit S, De Oliveira C, Schweighoffer F
European journal of biochemistry271(13)2584-92(2004 Jul)

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A
Genome research13(10)2265-70(2003 Oct)

NotI flanking sequences: a tool for gene discovery and verification of the human genome.
Kutsenko AS, Gizatullin RZ, Al-Amin AN, Wang F, Kvasha SM, Podowski RM, Matushkin YG, Gyanchandani A, Muravenko OV, Levitsky VG, Kolchanov NA, Protopopov AI, Kashuba VI, Kisselev LL, Wasserman W, Wahlestedt C, Zabarovsky ER
Nucleic acids research30(14)3163-70(2002 Jul)

Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16.
Daniels RJ, Peden JF, Lloyd C, Horsley SW, Clark K, Tufarelli C, Kearney L, Buckle VJ, Doggett NA, Flint J, Higgs DR
Human molecular genetics10(4)339-52(2001 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

suggests that CLP24 is a novel, hypoxically regulated tetraspan adherens junction protein that modulates cell adhesion, paracellular permeability and angiogenesis
Title: A novel four transmembrane spanning protein, CLP24. A hypoxically regulated cell junction protein.

The following TMEM204 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM204 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55860	NM_001256541.2 Latest version!	Homo sapiens transmembrane protein 204 (TMEM204), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu55860	NM_024600.6 Latest version!	Homo sapiens transmembrane protein 204 (TMEM204), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu55860	NM_024600.5	Homo sapiens transmembrane protein 204 (TMEM204), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu55860	NM_001256541.1	Homo sapiens transmembrane protein 204 (TMEM204), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM204 ( NM_024600.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_078876.2 Homo sapiens transmembrane protein 204 (TMEM204), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55860
Clone ID Related Accession (Same CDS sequence)	NM_024600.5 , NM_024600.6 , NM_001256541.2 , NM_001256541.1
Accession Version	NM_024600.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	transmembrane protein 204 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY676494.1, BC105785.1 and BQ182532.1. On Feb 8, 2012 this sequence version replaced NM_024600.4. Transcript Variant: This variant (1) represents the longer transcript. Both variants encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AY676494.1, ERR279827.4357.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGACCGTGC AGAGACTCGT GGCCGCGGCC GTGCTGGTGG CCCTGGTCTC ACTCATCCTC 
AACAACGTGG CGGCCTTCAC CTCCAACTGG GTGTGCCAGA CGCTGGAGGA TGGGCGCAGG 
CGCAGCGTGG GGCTGTGGAG GTCCTGCTGG CTGGTGGACA GGACCCGGGG AGGGCCGAGC 
CCTGGGGCCA GAGCCGGCCA GGTGGACGCA CATGACTGTG AGGCGCTGGG CTGGGGCTCC 
GAGGCAGCCG GCTTCCAGGA GTCCCGAGGC ACCGTCAAAC TGCAGTTCGA CATGATGCGC 
GCCTGCAACC TGGTGGCCAC GGCCGCGCTC ACCGCAGGCC AGCTCACCTT CCTCCTGGGG 
CTGGTGGGCC TGCCCCTGCT GTCACCCGAC GCCCCGTGCT GGGAGGAGGC CATGGCCGCT 
GCATTCCAAC TGGCGAGTTT TGTCCTGGTC ATCGGGCTCG TGACTTTCTA CAGAATTGGC 
CCATACACCA ACCTGTCCTG GTCCTGCTAC CTGAACATTG GCGCCTGCCT TCTGGCCACG 
CTGGCGGCAG CCATGCTCAT CTGGAACATT CTCCACAAGA GGGAGGACTG CATGGCCCCC 
CGGGTGATTG TCATCAGCCG CTCCCTGACA GCGCGCTTTC GCCGTGGGCT GGACAATGAC 
TACGTGGAGT CACCATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078876.2
CDS	620..1300
Translation	MTVQRLVAAAVLVALVSLILNNVAAFTSNWVCQTLEDGRRRSVGLWRSCWLVDRTRGGPSPGARAGQVDAHDCEALGWGSEAAGFQESRGTVKLQFDMMRACNLVATAALTAGQLTFLLGLVGLPLLSPDAPCWEEAMAAAFQLASFVLVIGLVTFYRIGPYTNLSWSCYLNIGACLLATLAAAMLIWNILHKREDCMAPRVIVISRSLTARFRRGLDNDYVESPC

Target ORF information:

RefSeq Version	NM_024600.5
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 204 (TMEM204), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024600.5

ORF Insert Sequence:

ATGACCGTGC AGAGACTCGT GGCCGCGGCC GTGCTGGTGG CCCTGGTCTC ACTCATCCTC 
AACAACGTGG CGGCCTTCAC CTCCAACTGG GTGTGCCAGA CGCTGGAGGA TGGGCGCAGG 
CGCAGCGTGG GGCTGTGGAG GTCCTGCTGG CTGGTGGACA GGACCCGGGG AGGGCCGAGC 
CCTGGGGCCA GAGCCGGCCA GGTGGACGCA CATGACTGTG AGGCGCTGGG CTGGGGCTCC 
GAGGCAGCCG GCTTCCAGGA GTCCCGAGGC ACCGTCAAAC TGCAGTTCGA CATGATGCGC 
GCCTGCAACC TGGTGGCCAC GGCCGCGCTC ACCGCAGGCC AGCTCACCTT CCTCCTGGGG 
CTGGTGGGCC TGCCCCTGCT GTCACCCGAC GCCCCGTGCT GGGAGGAGGC CATGGCCGCT 
GCATTCCAAC TGGCGAGTTT TGTCCTGGTC ATCGGGCTCG TGACTTTCTA CAGAATTGGC 
CCATACACCA ACCTGTCCTG GTCCTGCTAC CTGAACATTG GCGCCTGCCT TCTGGCCACG 
CTGGCGGCAG CCATGCTCAT CTGGAACATT CTCCACAAGA GGGAGGACTG CATGGCCCCC 
CGGGTGATTG TCATCAGCCG CTCCCTGACA GCGCGCTTTC GCCGTGGGCT GGACAATGAC 
TACGTGGAGT CACCATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM204 ( NM_024600.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_078876.2 Homo sapiens transmembrane protein 204 (TMEM204), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55860
Clone ID Related Accession (Same CDS sequence)	NM_024600.5 , NM_024600.6 , NM_001256541.2 , NM_001256541.1
Accession Version	NM_024600.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	transmembrane protein 204 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY676494.1 and BC105785.1. On Nov 22, 2018 this sequence version replaced NM_024600.5. Transcript Variant: This variant (1) represents the longer transcript. Both variants encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.76690.1, AY676494.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000566264.2/ ENSP00000454945.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCGTGC AGAGACTCGT GGCCGCGGCC GTGCTGGTGG CCCTGGTCTC ACTCATCCTC 
AACAACGTGG CGGCCTTCAC CTCCAACTGG GTGTGCCAGA CGCTGGAGGA TGGGCGCAGG 
CGCAGCGTGG GGCTGTGGAG GTCCTGCTGG CTGGTGGACA GGACCCGGGG AGGGCCGAGC 
CCTGGGGCCA GAGCCGGCCA GGTGGACGCA CATGACTGTG AGGCGCTGGG CTGGGGCTCC 
GAGGCAGCCG GCTTCCAGGA GTCCCGAGGC ACCGTCAAAC TGCAGTTCGA CATGATGCGC 
GCCTGCAACC TGGTGGCCAC GGCCGCGCTC ACCGCAGGCC AGCTCACCTT CCTCCTGGGG 
CTGGTGGGCC TGCCCCTGCT GTCACCCGAC GCCCCGTGCT GGGAGGAGGC CATGGCCGCT 
GCATTCCAAC TGGCGAGTTT TGTCCTGGTC ATCGGGCTCG TGACTTTCTA CAGAATTGGC 
CCATACACCA ACCTGTCCTG GTCCTGCTAC CTGAACATTG GCGCCTGCCT TCTGGCCACG 
CTGGCGGCAG CCATGCTCAT CTGGAACATT CTCCACAAGA GGGAGGACTG CATGGCCCCC 
CGGGTGATTG TCATCAGCCG CTCCCTGACA GCGCGCTTTC GCCGTGGGCT GGACAATGAC 
TACGTGGAGT CACCATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078876.2
CDS	611..1291
Translation	MTVQRLVAAAVLVALVSLILNNVAAFTSNWVCQTLEDGRRRSVGLWRSCWLVDRTRGGPSPGARAGQVDAHDCEALGWGSEAAGFQESRGTVKLQFDMMRACNLVATAALTAGQLTFLLGLVGLPLLSPDAPCWEEAMAAAFQLASFVLVIGLVTFYRIGPYTNLSWSCYLNIGACLLATLAAAMLIWNILHKREDCMAPRVIVISRSLTARFRRGLDNDYVESPC

Target ORF information:

RefSeq Version	NM_024600.6
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 204 (TMEM204), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024600.6

ORF Insert Sequence:

ATGACCGTGC AGAGACTCGT GGCCGCGGCC GTGCTGGTGG CCCTGGTCTC ACTCATCCTC 
AACAACGTGG CGGCCTTCAC CTCCAACTGG GTGTGCCAGA CGCTGGAGGA TGGGCGCAGG 
CGCAGCGTGG GGCTGTGGAG GTCCTGCTGG CTGGTGGACA GGACCCGGGG AGGGCCGAGC 
CCTGGGGCCA GAGCCGGCCA GGTGGACGCA CATGACTGTG AGGCGCTGGG CTGGGGCTCC 
GAGGCAGCCG GCTTCCAGGA GTCCCGAGGC ACCGTCAAAC TGCAGTTCGA CATGATGCGC 
GCCTGCAACC TGGTGGCCAC GGCCGCGCTC ACCGCAGGCC AGCTCACCTT CCTCCTGGGG 
CTGGTGGGCC TGCCCCTGCT GTCACCCGAC GCCCCGTGCT GGGAGGAGGC CATGGCCGCT 
GCATTCCAAC TGGCGAGTTT TGTCCTGGTC ATCGGGCTCG TGACTTTCTA CAGAATTGGC 
CCATACACCA ACCTGTCCTG GTCCTGCTAC CTGAACATTG GCGCCTGCCT TCTGGCCACG 
CTGGCGGCAG CCATGCTCAT CTGGAACATT CTCCACAAGA GGGAGGACTG CATGGCCCCC 
CGGGTGATTG TCATCAGCCG CTCCCTGACA GCGCGCTTTC GCCGTGGGCT GGACAATGAC 
TACGTGGAGT CACCATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM204 ( NM_001256541.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243470.1 Homo sapiens transmembrane protein 204 (TMEM204), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55860
Clone ID Related Accession (Same CDS sequence)	NM_024600.5 , NM_024600.6 , NM_001256541.2 , NM_001256541.1
Accession Version	NM_001256541.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-31
Organism	Homo sapiens(human)
Product	transmembrane protein 204 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL031719.12, BC004932.1 and BC105785.1. On Jun 1, 2019 this sequence version replaced NM_001256541.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BC004932.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END##

ATGACCGTGC AGAGACTCGT GGCCGCGGCC GTGCTGGTGG CCCTGGTCTC ACTCATCCTC 
AACAACGTGG CGGCCTTCAC CTCCAACTGG GTGTGCCAGA CGCTGGAGGA TGGGCGCAGG 
CGCAGCGTGG GGCTGTGGAG GTCCTGCTGG CTGGTGGACA GGACCCGGGG AGGGCCGAGC 
CCTGGGGCCA GAGCCGGCCA GGTGGACGCA CATGACTGTG AGGCGCTGGG CTGGGGCTCC 
GAGGCAGCCG GCTTCCAGGA GTCCCGAGGC ACCGTCAAAC TGCAGTTCGA CATGATGCGC 
GCCTGCAACC TGGTGGCCAC GGCCGCGCTC ACCGCAGGCC AGCTCACCTT CCTCCTGGGG 
CTGGTGGGCC TGCCCCTGCT GTCACCCGAC GCCCCGTGCT GGGAGGAGGC CATGGCCGCT 
GCATTCCAAC TGGCGAGTTT TGTCCTGGTC ATCGGGCTCG TGACTTTCTA CAGAATTGGC 
CCATACACCA ACCTGTCCTG GTCCTGCTAC CTGAACATTG GCGCCTGCCT TCTGGCCACG 
CTGGCGGCAG CCATGCTCAT CTGGAACATT CTCCACAAGA GGGAGGACTG CATGGCCCCC 
CGGGTGATTG TCATCAGCCG CTCCCTGACA GCGCGCTTTC GCCGTGGGCT GGACAATGAC 
TACGTGGAGT CACCATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243470.1
CDS	462..1142
Translation	MTVQRLVAAAVLVALVSLILNNVAAFTSNWVCQTLEDGRRRSVGLWRSCWLVDRTRGGPSPGARAGQVDAHDCEALGWGSEAAGFQESRGTVKLQFDMMRACNLVATAALTAGQLTFLLGLVGLPLLSPDAPCWEEAMAAAFQLASFVLVIGLVTFYRIGPYTNLSWSCYLNIGACLLATLAAAMLIWNILHKREDCMAPRVIVISRSLTARFRRGLDNDYVESPC

Target ORF information:

RefSeq Version	NM_001256541.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 204 (TMEM204), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256541.2

ORF Insert Sequence:

ATGACCGTGC AGAGACTCGT GGCCGCGGCC GTGCTGGTGG CCCTGGTCTC ACTCATCCTC 
AACAACGTGG CGGCCTTCAC CTCCAACTGG GTGTGCCAGA CGCTGGAGGA TGGGCGCAGG 
CGCAGCGTGG GGCTGTGGAG GTCCTGCTGG CTGGTGGACA GGACCCGGGG AGGGCCGAGC 
CCTGGGGCCA GAGCCGGCCA GGTGGACGCA CATGACTGTG AGGCGCTGGG CTGGGGCTCC 
GAGGCAGCCG GCTTCCAGGA GTCCCGAGGC ACCGTCAAAC TGCAGTTCGA CATGATGCGC 
GCCTGCAACC TGGTGGCCAC GGCCGCGCTC ACCGCAGGCC AGCTCACCTT CCTCCTGGGG 
CTGGTGGGCC TGCCCCTGCT GTCACCCGAC GCCCCGTGCT GGGAGGAGGC CATGGCCGCT 
GCATTCCAAC TGGCGAGTTT TGTCCTGGTC ATCGGGCTCG TGACTTTCTA CAGAATTGGC 
CCATACACCA ACCTGTCCTG GTCCTGCTAC CTGAACATTG GCGCCTGCCT TCTGGCCACG 
CTGGCGGCAG CCATGCTCAT CTGGAACATT CTCCACAAGA GGGAGGACTG CATGGCCCCC 
CGGGTGATTG TCATCAGCCG CTCCCTGACA GCGCGCTTTC GCCGTGGGCT GGACAATGAC 
TACGTGGAGT CACCATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM204 ( NM_001256541.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243470.1 Homo sapiens transmembrane protein 204 (TMEM204), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55860
Clone ID Related Accession (Same CDS sequence)	NM_024600.5 , NM_024600.6 , NM_001256541.2 , NM_001256541.1
Accession Version	NM_001256541.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	transmembrane protein 204 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC004932.1, BC105785.1 and BQ182532.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BC004932.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END##

ATGACCGTGC AGAGACTCGT GGCCGCGGCC GTGCTGGTGG CCCTGGTCTC ACTCATCCTC 
AACAACGTGG CGGCCTTCAC CTCCAACTGG GTGTGCCAGA CGCTGGAGGA TGGGCGCAGG 
CGCAGCGTGG GGCTGTGGAG GTCCTGCTGG CTGGTGGACA GGACCCGGGG AGGGCCGAGC 
CCTGGGGCCA GAGCCGGCCA GGTGGACGCA CATGACTGTG AGGCGCTGGG CTGGGGCTCC 
GAGGCAGCCG GCTTCCAGGA GTCCCGAGGC ACCGTCAAAC TGCAGTTCGA CATGATGCGC 
GCCTGCAACC TGGTGGCCAC GGCCGCGCTC ACCGCAGGCC AGCTCACCTT CCTCCTGGGG 
CTGGTGGGCC TGCCCCTGCT GTCACCCGAC GCCCCGTGCT GGGAGGAGGC CATGGCCGCT 
GCATTCCAAC TGGCGAGTTT TGTCCTGGTC ATCGGGCTCG TGACTTTCTA CAGAATTGGC 
CCATACACCA ACCTGTCCTG GTCCTGCTAC CTGAACATTG GCGCCTGCCT TCTGGCCACG 
CTGGCGGCAG CCATGCTCAT CTGGAACATT CTCCACAAGA GGGAGGACTG CATGGCCCCC 
CGGGTGATTG TCATCAGCCG CTCCCTGACA GCGCGCTTTC GCCGTGGGCT GGACAATGAC 
TACGTGGAGT CACCATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243470.1
CDS	399..1079
Translation	MTVQRLVAAAVLVALVSLILNNVAAFTSNWVCQTLEDGRRRSVGLWRSCWLVDRTRGGPSPGARAGQVDAHDCEALGWGSEAAGFQESRGTVKLQFDMMRACNLVATAALTAGQLTFLLGLVGLPLLSPDAPCWEEAMAAAFQLASFVLVIGLVTFYRIGPYTNLSWSCYLNIGACLLATLAAAMLIWNILHKREDCMAPRVIVISRSLTARFRRGLDNDYVESPC

Target ORF information:

RefSeq Version	NM_001256541.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 204 (TMEM204), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256541.1

ORF Insert Sequence:

ATGACCGTGC AGAGACTCGT GGCCGCGGCC GTGCTGGTGG CCCTGGTCTC ACTCATCCTC 
AACAACGTGG CGGCCTTCAC CTCCAACTGG GTGTGCCAGA CGCTGGAGGA TGGGCGCAGG 
CGCAGCGTGG GGCTGTGGAG GTCCTGCTGG CTGGTGGACA GGACCCGGGG AGGGCCGAGC 
CCTGGGGCCA GAGCCGGCCA GGTGGACGCA CATGACTGTG AGGCGCTGGG CTGGGGCTCC 
GAGGCAGCCG GCTTCCAGGA GTCCCGAGGC ACCGTCAAAC TGCAGTTCGA CATGATGCGC 
GCCTGCAACC TGGTGGCCAC GGCCGCGCTC ACCGCAGGCC AGCTCACCTT CCTCCTGGGG 
CTGGTGGGCC TGCCCCTGCT GTCACCCGAC GCCCCGTGCT GGGAGGAGGC CATGGCCGCT 
GCATTCCAAC TGGCGAGTTT TGTCCTGGTC ATCGGGCTCG TGACTTTCTA CAGAATTGGC 
CCATACACCA ACCTGTCCTG GTCCTGCTAC CTGAACATTG GCGCCTGCCT TCTGGCCACG 
CTGGCGGCAG CCATGCTCAT CTGGAACATT CTCCACAAGA GGGAGGACTG CATGGCCCCC 
CGGGTGATTG TCATCAGCCG CTCCCTGACA GCGCGCTTTC GCCGTGGGCT GGACAATGAC 
TACGTGGAGT CACCATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel four transmembrane spanning protein, CLP24. A hypoxically regulated cell junction protein.
European journal of biochemistry271(13)2584-92(2004 Jul)
Kearsey J,Petit S,De Oliveira C,Schweighoffer F

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Genome research13(10)2265-70(2003 Oct)
Clark HF,Gurney AL,Abaya E,Baker K,Baldwin D,Brush J,Chen J,Chow B,Chui C,Crowley C,Currell B,Deuel B,Dowd P,Eaton D,Foster J,Grimaldi C,Gu Q,Hass PE,Heldens S,Huang A,Kim HS,Klimowski L,Jin Y,Johnson S,Lee J,Lewis L,Liao D,Mark M,Robbie E,Sanchez C,Schoenfeld J,Seshagiri S,Simmons L,Singh J,Smith V,Stinson J,Vagts A,Vandlen R,Watanabe C,Wieand D,Woods K,Xie MH,Yansura D,Yi S,Yu G,Yuan J,Zhang M,Zhang Z,Goddard A,Wood WI,Godowski P,Gray A

NotI flanking sequences: a tool for gene discovery and verification of the human genome.
Nucleic acids research30(14)3163-70(2002 Jul)
Kutsenko AS,Gizatullin RZ,Al-Amin AN,Wang F,Kvasha SM,Podowski RM,Matushkin YG,Gyanchandani A,Muravenko OV,Levitsky VG,Kolchanov NA,Protopopov AI,Kashuba VI,Kisselev LL,Wasserman W,Wahlestedt C,Zabarovsky ER

Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16.
Human molecular genetics10(4)339-52(2001 Feb)
Daniels RJ,Peden JF,Lloyd C,Horsley SW,Clark K,Tufarelli C,Kearney L,Buckle VJ,Doggett NA,Flint J,Higgs DR

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TMEM204 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

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ORF Insert Sequence:

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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