MAGED4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAGED4
Entrez Gene ID	728239
Full Name	MAGE family member D4
Synonyms	MAGE-E1,MAGE1,MAGEE1
General protein information	Preferred Names MAGE family member D4 Names melanoma-associated antigen D4 MAGE-D4 antigen MAGE-E1 antigen melanoma antigen family D, 4 melanoma antigen family D4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.22
Disorder MIM:	300702

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272062.2	NP_001258991.1	melanoma-associated antigen D4 isoform 1
NM_001098800.3	NP_001092270.1	melanoma-associated antigen D4 isoform 2
NM_001098800.2	NP_001092270.1	melanoma-associated antigen D4 isoform 2
NM_001272061.1	NP_001258990.1	melanoma-associated antigen D4 isoform 3
NM_001272062.1	NP_001258991.1	melanoma-associated antigen D4 isoform 1
NM_001272063.1	NP_001258992.1	melanoma-associated antigen D4 isoform 1
NM_001272063.2	NP_001258992.1	melanoma-associated antigen D4 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAGED4	NP_001258991.1
Pan troglodytes (chimpanzee)	LOC465643	XP_003317515.2
	MAGED4B	XP_002806294.1
Bos taurus (cattle)	MAGED4B	XP_005228251.1
Homo sapiens (human)	MAGED4B	NP_110428.2
Canis lupus familiaris (dog)	LOC480926	XP_005641423.1

Gene Ontology
Bibliography

Related articles in PubMed

[Preparation and application of melanoma-associated antigen D4 polyclonal antibody].
He S, Gu Y, Xiao S, Zhang Q, Chen F, Luo B, Fu J, Xie X
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology33(1)104-108(2017 Jan)

Detection of serum melanoma-associated antigen D4 in patients with squamous cell carcinoma of the esophagus.
Oya H, Kanda M, Koike M, Iwata N, Niwa Y, Shimizu D, Takami H, Sueoka S, Hashimoto R, Ezaka K, Nomoto S, Yamada S, Fujii T, Nakayama G, Sugimoto H, Fujiwara M, Kodera Y
Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus29(6)663-9(2016 Aug)

Overexpression of MAGE-D4 in colorectal cancer is a potentially prognostic biomarker and immunotherapy target.
Zhang QM, He SJ, Shen N, Luo B, Fan R, Fu J, Luo GR, Zhou SF, Xiao SW, Xie XX
International journal of clinical and experimental pathology7(7)3918-27(2014)

High expression and frequently humoral immune response of melanoma-associated antigen D4 in glioma.
He SJ, Gu YY, Yu L, Luo B, Fan R, Lin WZ, Lan XW, Lin YD, Zhang QM, Xiao SW, Xie XX
International journal of clinical and experimental pathology7(5)2350-60(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The MAGE-D4 polyclonal antibody with high specificity and sensitivity has been successfully prepared.
Title: [Preparation and application of melanoma-associated antigen D4 polyclonal antibody].

Preoperative serum MAGED4 was significantly higher in esophageal squamous cell carcinoma patients than in controls.
Title: Detection of serum melanoma-associated antigen D4 in patients with squamous cell carcinoma of the esophagus.

MAGED4 (mRNA and protein) is frequently and highly expressed in glioma and is partly regulated by DNA methylation.
Title: MAGED4 expression in glioma and upregulation in glioma cell lines with 5-aza-2'-deoxycytidine treatment.

These findings suggest MAGE-D4 may serve as a potentially prognostic biomarker and an attractive target of immunotherapy in colorectal cancer
Title: Overexpression of MAGE-D4 in colorectal cancer is a potentially prognostic biomarker and immunotherapy target.

MAGE-D4 is highly expressed in glioma and can develop specifically humoral response in glioma patients.
Title: High expression and frequently humoral immune response of melanoma-associated antigen D4 in glioma.

The following MAGED4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGED4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05151	NM_001272061.1 Latest version!	Homo sapiens MAGE family member D4 (MAGED4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu05177	NM_001098800.3 Latest version!	Homo sapiens MAGE family member D4 (MAGED4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu05612	NM_001272063.2 Latest version!	Homo sapiens MAGE family member D4 (MAGED4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu05612	NM_001272062.2 Latest version!	Homo sapiens MAGE family member D4 (MAGED4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
View Old Accession Versions >>
OHu05177	NM_001098800.2	Homo sapiens MAGE family member D4 (MAGED4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu05612	NM_001272062.1	Homo sapiens MAGE family member D4 (MAGED4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu05612	NM_001272063.1	Homo sapiens MAGE family member D4 (MAGED4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAGED4 ( NM_001272061.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258990.1 Homo sapiens MAGE family member D4 (MAGED4), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu05151
Clone ID Related Accession (Same CDS sequence)	NM_001272061.1
Accession Version	NM_001272061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	melanoma-associated antigen D4 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC245177.1, AK289458.1 and BC063838.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (3). This transcript structure is inferred from aligning transcripts. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT 
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA 
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG 
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG 
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT 
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG 
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC 
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT 
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT 
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG 
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG 
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG 
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG 
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG 
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC 
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG 
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG 
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG 
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG 
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG 
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG 
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC 
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT 
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC 
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC 
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA 
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GAACCCGGAG 
CTCAGAGAGG AGACGCCTCT GAGCATGGCT CCCAGCTGGT ACCTGGAATA CAAGAAGATC 
CCCAACAGCA ACCCACCTGA GTATGAATTC CTCTGGGGCC TGCGAGCCCG CCATGAGACC 
AGCAAGATGA GGGTCCTGAG ATTCATCGCC CAGAATCAGA ACCGAGACCC CCGGGAATGG 
AAGGCTCATT TCTTGGAGGC TGTGGATGAT GCTTTCAAGA CAATGGATGT GGATATGGCC 
GAGGAACATG CCAGGGCCCA GATGAGGGCC CAGATGAATA TCGGGGATGA AGCGCTGATT 
GGACGGTGGA GCTGGGATGA CATACAAGTC GAGCTCCTGA CCTGGGATGA GGACGGAGAT 
TTTGGCGATG CCTGGGCCAG GATCCCCTTT GCTTTCTGGG CCAGATACCA TCAGTACATT 
CTGAATAGCA ACCGTGCCAA CAGGAGGGCC ACGTGGAGAG CTGGCGTCAG CAGTGGCACC 
AATGGAGGGG CCAGCACCAG CGTCCTAGAT GGCCCCAGCA CCAGCTCCAC CATCCGGACC 
AGAAATGCTG CCAGAGCTGG CGCCAGCTTC TTCTCCTGGA TCCAGCACCG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258990.1
CDS	196..2469
Translation	MAEGSFSVQSESYSVEDMDEGSDEVGEEEMVEGNDYEEFGAFGGYGTLTSFDIHILRAFGSLGPGLRILSNEPWELENPVLAQTLVEALQLDPETLANETAARAANVARAAASNRAARAAAAAARTAFSQVVASHRVATPQVSGEDTQPTTYAAEAQGPTPEPPLASPQTSQMLVTSKMAAPEAPATSAQSQTGSPAQEAATEGPSSACAFSQAPCAREVDANRPSTAFLGQNDVFDFTQPAGVSGMAFPRPKRPAPAQEAATEGPSAASGVPQTGPGREVAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPEGAAAATAQHSAEPWARMGGKRTKKSKHLDDEYESSEEERETPAVPPTWRASQPSLTVRAQLAPRPPMAPRSQIPSRHVLCLPPRNVTLLQERANKLVKYLMIKDYKKIPIKRADMLKDVIREYDEHFPEIIERATYTLEKKFGIHLKEIDKEEHLYILVCTRDSSARLLGKTKDTPRLSLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPFLGDLRKLITDDFVKQKNPELREETPLSMAPSWYLEYKKIPNSNPPEYEFLWGLRARHETSKMRVLRFIAQNQNRDPREWKAHFLEAVDDAFKTMDVDMAEEHARAQMRAQMNIGDEALIGRWSWDDIQVELLTWDEDGDFGDAWARIPFAFWARYHQYILNSNRANRRATWRAGVSSGTNGGASTSVLDGPSTSSTIRTRNAARAGASFFSWIQHR

Target ORF information:

RefSeq Version	NM_001272061.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member D4 (MAGED4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272061.1

ORF Insert Sequence:

ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT 
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA 
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG 
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG 
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT 
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG 
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC 
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT 
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT 
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG 
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG 
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG 
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG 
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG 
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC 
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG 
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG 
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG 
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG 
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG 
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG 
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC 
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT 
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC 
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC 
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA 
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GAACCCGGAG 
CTCAGAGAGG AGACGCCTCT GAGCATGGCT CCCAGCTGGT ACCTGGAATA CAAGAAGATC 
CCCAACAGCA ACCCACCTGA GTATGAATTC CTCTGGGGCC TGCGAGCCCG CCATGAGACC 
AGCAAGATGA GGGTCCTGAG ATTCATCGCC CAGAATCAGA ACCGAGACCC CCGGGAATGG 
AAGGCTCATT TCTTGGAGGC TGTGGATGAT GCTTTCAAGA CAATGGATGT GGATATGGCC 
GAGGAACATG CCAGGGCCCA GATGAGGGCC CAGATGAATA TCGGGGATGA AGCGCTGATT 
GGACGGTGGA GCTGGGATGA CATACAAGTC GAGCTCCTGA CCTGGGATGA GGACGGAGAT 
TTTGGCGATG CCTGGGCCAG GATCCCCTTT GCTTTCTGGG CCAGATACCA TCAGTACATT 
CTGAATAGCA ACCGTGCCAA CAGGAGGGCC ACGTGGAGAG CTGGCGTCAG CAGTGGCACC 
AATGGAGGGG CCAGCACCAG CGTCCTAGAT GGCCCCAGCA CCAGCTCCAC CATCCGGACC 
AGAAATGCTG CCAGAGCTGG CGCCAGCTTC TTCTCCTGGA TCCAGCACCG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED4 ( NM_001098800.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092270.1 Homo sapiens MAGE family member D4 (MAGED4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu05177
Clone ID Related Accession (Same CDS sequence)	NM_001098800.3 , NM_001098800.2
Accession Version	NM_001098800.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	melanoma-associated antigen D4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK289458.1, BC063838.1 and AC245177.1. On Jun 2, 2019 this sequence version replaced NM_001098800.2. Transcript Variant: This variant (3) differs in the 5' UTR, lacks an alternate exon, and uses an alternate splice site in the 3' coding region, compared to variant 4. This results in a frameshift and an early stop codon. The encoded protein (isoform 2) is shorter and has a distinct C-terminus, compared to isoform 3. This transcript structure is inferred from aligning transcripts. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.271547.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT 
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA 
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG 
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG 
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT 
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG 
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC 
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT 
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT 
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG 
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG 
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG 
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG 
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG 
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC 
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG 
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG 
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG 
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG 
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG 
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG 
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC 
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT 
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC 
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC 
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA 
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA 
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC 
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC 
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT 
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT 
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT 
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC 
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC 
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC 
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092270.1
CDS	80..2299
Translation	MAEGSFSVQSESYSVEDMDEGSDEVGEEEMVEGNDYEEFGAFGGYGTLTSFDIHILRAFGSLGPGLRILSNEPWELENPVLAQTLVEALQLDPETLANETAARAANVARAAASNRAARAAAAAARTAFSQVVASHRVATPQVSGEDTQPTTYAAEAQGPTPEPPLASPQTSQMLVTSKMAAPEAPATSAQSQTGSPAQEAATEGPSSACAFSQAPCAREVDANRPSTAFLGQNDVFDFTQPAGVSGMAFPRPKRPAPAQEAATEGPSAASGVPQTGPGREVAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPEGAAAATAQHSAEPWARMGGKRTKKSKHLDDEYESSEEERETPAVPPTWRASQPSLTVRAQLAPRPPMAPRSQIPSRHVLCLPPRNVTLLQERANKLVKYLMIKDYKKIPIKRADMLKDVIREYDEHFPEIIERATYTLEKKFGIHLKEIDKEEHLYILVCTRDSSARLLGKTKDTPRLSLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPFLGDLRKLITDDFVKQKYLEYKKIPNSNPPEYEFLWGLRARHETSKMRVLRFIAQNQNRDPREWKAHFLEAVDDAFKTMDVDMAEEHARAQMRAQMNIGDEALIGRWSWDDIQVELLTWDEDGDFGDAWARIPFAFWARYHQYILNSNRANRRATWRAGVSSGTNGGASTSVLDGPSTSSTIRTRNAARAGASFFSWIQ

Target ORF information:

RefSeq Version	NM_001098800.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member D4 (MAGED4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098800.3

ORF Insert Sequence:

ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT 
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA 
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG 
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG 
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT 
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG 
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC 
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT 
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT 
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG 
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG 
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG 
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG 
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG 
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC 
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG 
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG 
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG 
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG 
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG 
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG 
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC 
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT 
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC 
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC 
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA 
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA 
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC 
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC 
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT 
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT 
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT 
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC 
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC 
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC 
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED4 ( NM_001098800.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092270.1 Homo sapiens MAGE family member D4 (MAGED4), transcript variant 3, mRNA.

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CloneID	OHu05177
Clone ID Related Accession (Same CDS sequence)	NM_001098800.3 , NM_001098800.2
Accession Version	NM_001098800.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-14
Organism	Homo sapiens(human)
Product	melanoma-associated antigen D4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC245177.1, AK289458.1 and BC063838.1. On Jan 5, 2013 this sequence version replaced NM_001098800.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks an alternate exon, and uses an alternate splice site in the 3' coding region, compared to variant 4. This results in a frameshift and an early stop codon. The encoded protein (isoform 2) is shorter and has a distinct C-terminus, compared to isoform 3. This transcript structure is inferred from aligning transcripts. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.271547.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT 
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA 
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG 
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG 
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT 
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG 
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC 
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT 
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT 
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG 
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG 
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG 
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG 
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG 
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC 
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG 
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG 
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG 
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG 
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG 
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG 
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC 
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT 
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC 
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC 
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA 
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA 
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC 
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC 
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT 
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT 
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT 
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC 
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC 
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC 
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092270.1
CDS	145..2364
Translation	MAEGSFSVQSESYSVEDMDEGSDEVGEEEMVEGNDYEEFGAFGGYGTLTSFDIHILRAFGSLGPGLRILSNEPWELENPVLAQTLVEALQLDPETLANETAARAANVARAAASNRAARAAAAAARTAFSQVVASHRVATPQVSGEDTQPTTYAAEAQGPTPEPPLASPQTSQMLVTSKMAAPEAPATSAQSQTGSPAQEAATEGPSSACAFSQAPCAREVDANRPSTAFLGQNDVFDFTQPAGVSGMAFPRPKRPAPAQEAATEGPSAASGVPQTGPGREVAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPEGAAAATAQHSAEPWARMGGKRTKKSKHLDDEYESSEEERETPAVPPTWRASQPSLTVRAQLAPRPPMAPRSQIPSRHVLCLPPRNVTLLQERANKLVKYLMIKDYKKIPIKRADMLKDVIREYDEHFPEIIERATYTLEKKFGIHLKEIDKEEHLYILVCTRDSSARLLGKTKDTPRLSLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPFLGDLRKLITDDFVKQKYLEYKKIPNSNPPEYEFLWGLRARHETSKMRVLRFIAQNQNRDPREWKAHFLEAVDDAFKTMDVDMAEEHARAQMRAQMNIGDEALIGRWSWDDIQVELLTWDEDGDFGDAWARIPFAFWARYHQYILNSNRANRRATWRAGVSSGTNGGASTSVLDGPSTSSTIRTRNAARAGASFFSWIQ

Target ORF information:

RefSeq Version	NM_001098800.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member D4 (MAGED4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098800.2

ORF Insert Sequence:

ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT 
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA 
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG 
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG 
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT 
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG 
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC 
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT 
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT 
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG 
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG 
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG 
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG 
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG 
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC 
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG 
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG 
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG 
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG 
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG 
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG 
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC 
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT 
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC 
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC 
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA 
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA 
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC 
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC 
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT 
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT 
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT 
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC 
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC 
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC 
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED4 ( NM_001272062.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258991.1 Homo sapiens MAGE family member D4 (MAGED4), transcript variant 2, mRNA.

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CloneID	OHu05612
Clone ID Related Accession (Same CDS sequence)	NM_001272062.2 , NM_001272062.1 , NM_001272063.1 , NM_001272063.2
Accession Version	NM_001272062.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	melanoma-associated antigen D4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK289458.1 and BC063838.1. On Jun 2, 2019 this sequence version replaced NM_001272062.1. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate exon in the coding region, compared to variant 4. It encodes isoform 1, which is shorter than isoform 3. Variants 1 and 2 encode the same isoform (1). This transcript structure is inferred from aligning transcripts. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.70897.1, SRR1803613.54764.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT 
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA 
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG 
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG 
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT 
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG 
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC 
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT 
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT 
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG 
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG 
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG 
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG 
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG 
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC 
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG 
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG 
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG 
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG 
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG 
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG 
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC 
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT 
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC 
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC 
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA 
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA 
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC 
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC 
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT 
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT 
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT 
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC 
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC 
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC 
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGCAC 
CGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258991.1
CDS	80..2305
Translation	MAEGSFSVQSESYSVEDMDEGSDEVGEEEMVEGNDYEEFGAFGGYGTLTSFDIHILRAFGSLGPGLRILSNEPWELENPVLAQTLVEALQLDPETLANETAARAANVARAAASNRAARAAAAAARTAFSQVVASHRVATPQVSGEDTQPTTYAAEAQGPTPEPPLASPQTSQMLVTSKMAAPEAPATSAQSQTGSPAQEAATEGPSSACAFSQAPCAREVDANRPSTAFLGQNDVFDFTQPAGVSGMAFPRPKRPAPAQEAATEGPSAASGVPQTGPGREVAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPEGAAAATAQHSAEPWARMGGKRTKKSKHLDDEYESSEEERETPAVPPTWRASQPSLTVRAQLAPRPPMAPRSQIPSRHVLCLPPRNVTLLQERANKLVKYLMIKDYKKIPIKRADMLKDVIREYDEHFPEIIERATYTLEKKFGIHLKEIDKEEHLYILVCTRDSSARLLGKTKDTPRLSLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPFLGDLRKLITDDFVKQKYLEYKKIPNSNPPEYEFLWGLRARHETSKMRVLRFIAQNQNRDPREWKAHFLEAVDDAFKTMDVDMAEEHARAQMRAQMNIGDEALIGRWSWDDIQVELLTWDEDGDFGDAWARIPFAFWARYHQYILNSNRANRRATWRAGVSSGTNGGASTSVLDGPSTSSTIRTRNAARAGASFFSWIQHR

Target ORF information:

RefSeq Version	NM_001272062.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member D4 (MAGED4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272062.2

ORF Insert Sequence:

ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT 
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA 
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG 
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG 
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT 
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG 
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC 
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT 
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT 
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG 
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG 
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG 
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG 
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG 
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC 
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG 
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG 
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG 
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG 
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG 
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG 
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC 
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT 
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC 
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC 
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA 
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA 
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC 
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC 
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT 
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT 
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT 
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC 
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC 
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC 
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGCAC 
CGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED4 ( NM_001272062.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258991.1 Homo sapiens MAGE family member D4 (MAGED4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu05612
Clone ID Related Accession (Same CDS sequence)	NM_001272062.2 , NM_001272062.1 , NM_001272063.1 , NM_001272063.2
Accession Version	NM_001272062.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-14
Organism	Homo sapiens(human)
Product	melanoma-associated antigen D4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC245177.1, AK289458.1 and BC063838.1. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate exon in the coding region, compared to variant 4. It encodes isoform 1, which is shorter than isoform 3. Variants 1 and 2 encode the same isoform (1). This transcript structure is inferred from aligning transcripts. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.54764.1, SRR1660809.138385.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT 
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA 
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG 
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG 
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT 
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG 
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC 
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT 
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT 
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG 
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG 
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG 
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG 
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG 
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC 
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG 
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG 
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG 
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG 
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG 
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG 
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC 
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT 
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC 
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC 
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA 
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA 
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC 
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC 
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT 
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT 
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT 
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC 
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC 
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC 
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGCAC 
CGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258991.1
CDS	145..2370
Translation	MAEGSFSVQSESYSVEDMDEGSDEVGEEEMVEGNDYEEFGAFGGYGTLTSFDIHILRAFGSLGPGLRILSNEPWELENPVLAQTLVEALQLDPETLANETAARAANVARAAASNRAARAAAAAARTAFSQVVASHRVATPQVSGEDTQPTTYAAEAQGPTPEPPLASPQTSQMLVTSKMAAPEAPATSAQSQTGSPAQEAATEGPSSACAFSQAPCAREVDANRPSTAFLGQNDVFDFTQPAGVSGMAFPRPKRPAPAQEAATEGPSAASGVPQTGPGREVAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPEGAAAATAQHSAEPWARMGGKRTKKSKHLDDEYESSEEERETPAVPPTWRASQPSLTVRAQLAPRPPMAPRSQIPSRHVLCLPPRNVTLLQERANKLVKYLMIKDYKKIPIKRADMLKDVIREYDEHFPEIIERATYTLEKKFGIHLKEIDKEEHLYILVCTRDSSARLLGKTKDTPRLSLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPFLGDLRKLITDDFVKQKYLEYKKIPNSNPPEYEFLWGLRARHETSKMRVLRFIAQNQNRDPREWKAHFLEAVDDAFKTMDVDMAEEHARAQMRAQMNIGDEALIGRWSWDDIQVELLTWDEDGDFGDAWARIPFAFWARYHQYILNSNRANRRATWRAGVSSGTNGGASTSVLDGPSTSSTIRTRNAARAGASFFSWIQHR

Target ORF information:

RefSeq Version	NM_001272062.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member D4 (MAGED4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272062.1

ORF Insert Sequence:

ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT 
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA 
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG 
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG 
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT 
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG 
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC 
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT 
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT 
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG 
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG 
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG 
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG 
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG 
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC 
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG 
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG 
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG 
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG 
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG 
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG 
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC 
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT 
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC 
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC 
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA 
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA 
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC 
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC 
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT 
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT 
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT 
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC 
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC 
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC 
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGCAC 
CGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED4 ( NM_001272063.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258992.1 Homo sapiens MAGE family member D4 (MAGED4), transcript variant 1, mRNA.

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CloneID	OHu05612
Clone ID Related Accession (Same CDS sequence)	NM_001272062.2 , NM_001272062.1 , NM_001272063.1 , NM_001272063.2
Accession Version	NM_001272063.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-03
Organism	Homo sapiens(human)
Product	melanoma-associated antigen D4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC245177.1, AK289458.1 and BC063838.1. Transcript Variant: This variant (1) differs in the 5' UTR and lacks an alternate exon in the coding region, compared to variant 4. It encodes isoform 1, which is shorter than isoform 3. Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660807.216343.1, SRR1660809.204031.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT 
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA 
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG 
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG 
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT 
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG 
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC 
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT 
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT 
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG 
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG 
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG 
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG 
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG 
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC 
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG 
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG 
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG 
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG 
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG 
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG 
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC 
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT 
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC 
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC 
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA 
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA 
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC 
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC 
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT 
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT 
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT 
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC 
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC 
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC 
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGCAC 
CGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258992.1
CDS	175..2400
Translation	MAEGSFSVQSESYSVEDMDEGSDEVGEEEMVEGNDYEEFGAFGGYGTLTSFDIHILRAFGSLGPGLRILSNEPWELENPVLAQTLVEALQLDPETLANETAARAANVARAAASNRAARAAAAAARTAFSQVVASHRVATPQVSGEDTQPTTYAAEAQGPTPEPPLASPQTSQMLVTSKMAAPEAPATSAQSQTGSPAQEAATEGPSSACAFSQAPCAREVDANRPSTAFLGQNDVFDFTQPAGVSGMAFPRPKRPAPAQEAATEGPSAASGVPQTGPGREVAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPEGAAAATAQHSAEPWARMGGKRTKKSKHLDDEYESSEEERETPAVPPTWRASQPSLTVRAQLAPRPPMAPRSQIPSRHVLCLPPRNVTLLQERANKLVKYLMIKDYKKIPIKRADMLKDVIREYDEHFPEIIERATYTLEKKFGIHLKEIDKEEHLYILVCTRDSSARLLGKTKDTPRLSLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPFLGDLRKLITDDFVKQKYLEYKKIPNSNPPEYEFLWGLRARHETSKMRVLRFIAQNQNRDPREWKAHFLEAVDDAFKTMDVDMAEEHARAQMRAQMNIGDEALIGRWSWDDIQVELLTWDEDGDFGDAWARIPFAFWARYHQYILNSNRANRRATWRAGVSSGTNGGASTSVLDGPSTSSTIRTRNAARAGASFFSWIQHR

Target ORF information:

RefSeq Version	NM_001272063.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member D4 (MAGED4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272063.1

ORF Insert Sequence:

ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT 
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA 
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG 
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG 
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT 
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG 
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC 
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT 
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT 
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG 
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG 
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG 
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG 
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG 
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC 
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG 
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG 
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG 
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG 
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG 
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG 
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC 
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT 
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC 
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC 
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA 
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA 
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC 
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC 
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT 
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT 
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT 
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC 
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC 
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC 
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGCAC 
CGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED4 ( NM_001272063.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258992.1 Homo sapiens MAGE family member D4 (MAGED4), transcript variant 1, mRNA.

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CloneID	OHu05612
Clone ID Related Accession (Same CDS sequence)	NM_001272062.2 , NM_001272062.1 , NM_001272063.1 , NM_001272063.2
Accession Version	NM_001272063.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	melanoma-associated antigen D4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK289458.1 and BC063838.1. On Jun 2, 2019 this sequence version replaced NM_001272063.1. Transcript Variant: This variant (1) differs in the 5' UTR and lacks an alternate exon in the coding region, compared to variant 4. It encodes isoform 1, which is shorter than isoform 3. Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660805.135415.1, SRR7410570.689571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT 
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA 
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG 
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG 
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT 
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG 
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC 
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT 
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT 
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG 
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG 
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG 
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG 
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG 
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC 
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG 
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG 
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG 
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG 
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG 
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG 
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC 
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT 
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC 
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC 
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA 
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA 
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC 
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC 
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT 
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT 
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT 
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC 
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC 
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC 
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGCAC 
CGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258992.1
CDS	110..2335
Translation	MAEGSFSVQSESYSVEDMDEGSDEVGEEEMVEGNDYEEFGAFGGYGTLTSFDIHILRAFGSLGPGLRILSNEPWELENPVLAQTLVEALQLDPETLANETAARAANVARAAASNRAARAAAAAARTAFSQVVASHRVATPQVSGEDTQPTTYAAEAQGPTPEPPLASPQTSQMLVTSKMAAPEAPATSAQSQTGSPAQEAATEGPSSACAFSQAPCAREVDANRPSTAFLGQNDVFDFTQPAGVSGMAFPRPKRPAPAQEAATEGPSAASGVPQTGPGREVAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPEGAAAATAQHSAEPWARMGGKRTKKSKHLDDEYESSEEERETPAVPPTWRASQPSLTVRAQLAPRPPMAPRSQIPSRHVLCLPPRNVTLLQERANKLVKYLMIKDYKKIPIKRADMLKDVIREYDEHFPEIIERATYTLEKKFGIHLKEIDKEEHLYILVCTRDSSARLLGKTKDTPRLSLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPFLGDLRKLITDDFVKQKYLEYKKIPNSNPPEYEFLWGLRARHETSKMRVLRFIAQNQNRDPREWKAHFLEAVDDAFKTMDVDMAEEHARAQMRAQMNIGDEALIGRWSWDDIQVELLTWDEDGDFGDAWARIPFAFWARYHQYILNSNRANRRATWRAGVSSGTNGGASTSVLDGPSTSSTIRTRNAARAGASFFSWIQHR

Target ORF information:

RefSeq Version	NM_001272063.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member D4 (MAGED4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272063.2

ORF Insert Sequence:

ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT 
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA 
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG 
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG 
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT 
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG 
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC 
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT 
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT 
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG 
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG 
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG 
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG 
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG 
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC 
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG 
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG 
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG 
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG 
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG 
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG 
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC 
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT 
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC 
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC 
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA 
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA 
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC 
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC 
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT 
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT 
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT 
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC 
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC 
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC 
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGCAC 
CGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[Preparation and application of melanoma-associated antigen D4 polyclonal antibody].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology33(1)104-108(2017 Jan)
He S,Gu Y,Xiao S,Zhang Q,Chen F,Luo B,Fu J,Xie X

Detection of serum melanoma-associated antigen D4 in patients with squamous cell carcinoma of the esophagus.
Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus29(6)663-9(2016 Aug)
Oya H,Kanda M,Koike M,Iwata N,Niwa Y,Shimizu D,Takami H,Sueoka S,Hashimoto R,Ezaka K,Nomoto S,Yamada S,Fujii T,Nakayama G,Sugimoto H,Fujiwara M,Kodera Y

Overexpression of MAGE-D4 in colorectal cancer is a potentially prognostic biomarker and immunotherapy target.
International journal of clinical and experimental pathology7(7)3918-27(2014)
Zhang QM,He SJ,Shen N,Luo B,Fan R,Fu J,Luo GR,Zhou SF,Xiao SW,Xie XX

High expression and frequently humoral immune response of melanoma-associated antigen D4 in glioma.
International journal of clinical and experimental pathology7(5)2350-60(2014)
He SJ,Gu YY,Yu L,Luo B,Fan R,Lin WZ,Lan XW,Lin YD,Zhang QM,Xiao SW,Xie XX

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MAGED4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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