MAGED4B cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAGED4B
Entrez Gene ID	100125231
Full Name	melanoma antigen family D, 4B
Synonyms	MAGED4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024987698.1	XP_024843466.1	melanoma-associated antigen D4 isoform X1
XM_024987700.1	XP_024843468.1	melanoma-associated antigen D4 isoform X2
XM_015461704.1	XP_015317190.1	melanoma-associated antigen D4 isoform X1
XM_015461704.2	XP_015317190.2	melanoma-associated antigen D4 isoform X1
XM_024987699.1	XP_024843467.1	melanoma-associated antigen D4 isoform X1
NM_001103311.1	NP_001096781.1	melanoma-associated antigen D4
XM_024987701.1	XP_024843469.1	melanoma-associated antigen D4 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAGED4	NP_001258991.1
Pan troglodytes (chimpanzee)	LOC465643	XP_003317515.2
	MAGED4B	XP_002806294.1
Bos taurus (cattle)	MAGED4B	XP_005228251.1
Homo sapiens (human)	MAGED4B	NP_110428.2
Canis lupus familiaris (dog)	LOC480926	XP_005641423.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAGED4B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGED4B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa44084	NM_001103311.1 Latest version!	Bos taurus melanoma antigen family D, 4B (MAGED4B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa238221	XM_024987698.1 Latest version!	Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa238221	XM_015461704.2 Latest version!	Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa238221	XM_024987699.1 Latest version!	Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa238222	XM_024987701.1 Latest version!	Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa238222	XM_024987700.1 Latest version!	Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa89389	XM_015461704.1 Latest version!	Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAGED4B ( NM_001103311.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001096781.1 Bos taurus melanoma antigen family D, 4B (MAGED4B), mRNA.

Price & Availability↑

CloneID	OBa44084
Clone ID Related Accession (Same CDS sequence)	NM_001103311.1
Accession Version	NM_001103311.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	melanoma-associated antigen D4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC147977.1. ##Evidence-Data-START## Transcript exon combination :: BC147977.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGAGG GAAGCTACCG CAAGGAATCT GAAGGGTACA ACGTTGAAGA CATGGACGAG 
GGTAGTGATG AAGTCGGGGA GGAAGACATG GTTGAAGGCA ACGACTATGA AGAATTTGGT 
GCTTTCGGAG GCTACGGCGC CCTCACCAGC TTTGACATCC GCATCCTCAG AGCCTTTGGG 
AGCTTGGGTC CAGGCTTTCG CATCTTAGCG AATGAGCCCT GGGAACTGGA AAACCCAGTG 
CTGGCCAGAA CTCTGCTGGA GGCATTTCGG ATGGATCCAG AAACACTTGC CAATGAGACG 
GCTGCCCGTG CTGCCAACGT AGCCCGGGCC GCCGCCTCTA ACCAAGCTGC TCGGGCCGCT 
GCCACTGCTG CCCGTGCCAC CTACAATCAG GTGGTCACTA ACCACCACCC GGTGGCCACA 
CACCAGGCGT CAGGAGGCGA TACCCAGCCC ATGACATCTG CTGCCCAGGC TCCGGCAGCC 
ACCCCTGAGA CAAGCGTCGC TTCTCCGCAC AGCTCCCGGA TGCTAGTCAA TAGCGAGATG 
GCTGCCCCTG GGGCTCCAGC AAGGTCTCCA CAGCCGCAGA CATCCTCCCA GGCCCAGGAG 
GCTGCGGCCG AGGGCCCTAG TACGGCCTGT GCTTTCCCCC AGGCCTCGCG TGCAAGTGAG 
ATGGATGCCA CCCGGCCCAA GACAGCCTTC CTGGGTCAGA ACGACGCCTT TGATTTCAGC 
CAGCCGGCAG GTGTCAGTGG CATGGCCTTC CCACGCCCCA AGAGACCTGC CCCGGCCCAA 
GAGGCTGCCA CAGAGGGCCC CAGTGTTGCC TCCAGGGGCA CCCAGGCAGC CTCTGCCGGG 
GAGGGGGCGG CCACCCGGCC CAAGACGACC AAGTCCGGGA AGGCTCTCGC CAAGACTCGC 
TGGGTGGAGC CTCAGAATGT TGTGGCAGCA GCTGCCGCCA AGGCCAAGAT GGCCACGAGC 
ATCCCTGAGC CTGAGAGTGC AGCTGCCACT TCTCAGCAGA GTGCGGAGCC CTGGGCCAGA 
ATGGGAGGCA AGAGGACAAA GAAGTCCAAG CACCTGGATG ACGAATATGA GAGCGGCGAG 
GAGGAGAGAG AGCCTCCTGC GGTCCCACCA ACCTGGAGGG CATCGCAGCC CCTGCTGACG 
ACTGCGCGGC CTCAGGTGGC CCCTCGGCCC TCCATGGCCC TGAGGTCCCA GGTACCCTCA 
AGGCATGTGC TGTGCTTGCC ACCCCGCAAT GTGACCCTTC TGCAAGAGAG GGCAAATAAG 
TTGGTGAAAT ATCTGATGAT TAAAGACTAC AAGAAGATCC CCATCAAGCG CTCAGACATG 
CTGAAGGATG TCATCCGAGA ATATGACGAA CACTTCCCTG AGATCATTGA ACGAGCAACG 
TACACTCTGG AAAAGAAGTT CGGGATCCAC CTGAAGGAAA TTGACAAGGA AGAGCACCTG 
TACATTCTCG TCTGCACACG GGATTCGTCA GCCCGCCTCC TGGGAAAGAC CAAGGATACT 
CCCAGGCTGA GTCTCCTCTT GGTGATTCTG GGTGTCATCT TCATGAACGG CAACCGCGCC 
AGCGAGGCTG TCCTCTGGGA GGCACTCCGC AAGATGGGAC TGCGCCCCGG GGTGAGGCAC 
CCATTCCTTG GCGATCTGAG GAAACTCATT ACAGAGGACT TTGTGAAGCA GAAGTACTTG 
GAATACAAGA AGGTCCCCAA TAGCAGCCCA CCCGAGTATG AGTTTCTCTG GGGTCTGCGC 
GCCTGCCATG AGACCAGCAA GATGAGGGTG CTGAGATTCA TCGCCCAGTA TCAGAACCGA 
GACCCCCGGG AATGGAGGGC TCATTTCTTG GAGGCTGTGG ATGATGCTTT CAAGACGATG 
GATGTGGATA TGGCCGAGGA GCATGCCAGG GCCCAGATGA GGGCCCAGAT GAACATCGGG 
GAGGAAGCTC TGATTGGACG GTGGAGCTGG GACGATATCC AGGTGGAGCT CCTGACCTGG 
GATGAGGACG GAGATTTTGG CGACGCCTGG TCCAGGATCC CCTTCGCTTT CTGGGCCAGA 
TACCATCAGT ACATTCTGAA TAGCAACCGT CCCAACAGGA GAGGTACCTG GAGGGCTGGT 
GTCAGCAGTG GCACCAATGG CGCGGCCAGC GCCAGCATGC TCGATGGCCC CAGCACCAGC 
TCCACCATCC GGACCAGAAA CGCCGCCAGA ACTAGTGCCA GCTTCTTCTC CTGGATTCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096781.1
CDS	231..2453
Translation	MAEGSYRKESEGYNVEDMDEGSDEVGEEDMVEGNDYEEFGAFGGYGALTSFDIRILRAFGSLGPGFRILANEPWELENPVLARTLLEAFRMDPETLANETAARAANVARAAASNQAARAAATAARATYNQVVTNHHPVATHQASGGDTQPMTSAAQAPAATPETSVASPHSSRMLVNSEMAAPGAPARSPQPQTSSQAQEAAAEGPSTACAFPQASRASEMDATRPKTAFLGQNDAFDFSQPAGVSGMAFPRPKRPAPAQEAATEGPSVASRGTQAASAGEGAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPESAAATSQQSAEPWARMGGKRTKKSKHLDDEYESGEEEREPPAVPPTWRASQPLLTTARPQVAPRPSMALRSQVPSRHVLCLPPRNVTLLQERANKLVKYLMIKDYKKIPIKRSDMLKDVIREYDEHFPEIIERATYTLEKKFGIHLKEIDKEEHLYILVCTRDSSARLLGKTKDTPRLSLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPFLGDLRKLITEDFVKQKYLEYKKVPNSSPPEYEFLWGLRACHETSKMRVLRFIAQYQNRDPREWRAHFLEAVDDAFKTMDVDMAEEHARAQMRAQMNIGEEALIGRWSWDDIQVELLTWDEDGDFGDAWSRIPFAFWARYHQYILNSNRPNRRGTWRAGVSSGTNGAASASMLDGPSTSSTIRTRNAARTSASFFSWIQ

Target ORF information:

RefSeq Version	NM_001103311.1
Organism	Bos taurus(cattle)
Definition	Bos taurus melanoma antigen family D, 4B (MAGED4B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103311.1

ORF Insert Sequence:

ATGGCAGAGG GAAGCTACCG CAAGGAATCT GAAGGGTACA ACGTTGAAGA CATGGACGAG 
GGTAGTGATG AAGTCGGGGA GGAAGACATG GTTGAAGGCA ACGACTATGA AGAATTTGGT 
GCTTTCGGAG GCTACGGCGC CCTCACCAGC TTTGACATCC GCATCCTCAG AGCCTTTGGG 
AGCTTGGGTC CAGGCTTTCG CATCTTAGCG AATGAGCCCT GGGAACTGGA AAACCCAGTG 
CTGGCCAGAA CTCTGCTGGA GGCATTTCGG ATGGATCCAG AAACACTTGC CAATGAGACG 
GCTGCCCGTG CTGCCAACGT AGCCCGGGCC GCCGCCTCTA ACCAAGCTGC TCGGGCCGCT 
GCCACTGCTG CCCGTGCCAC CTACAATCAG GTGGTCACTA ACCACCACCC GGTGGCCACA 
CACCAGGCGT CAGGAGGCGA TACCCAGCCC ATGACATCTG CTGCCCAGGC TCCGGCAGCC 
ACCCCTGAGA CAAGCGTCGC TTCTCCGCAC AGCTCCCGGA TGCTAGTCAA TAGCGAGATG 
GCTGCCCCTG GGGCTCCAGC AAGGTCTCCA CAGCCGCAGA CATCCTCCCA GGCCCAGGAG 
GCTGCGGCCG AGGGCCCTAG TACGGCCTGT GCTTTCCCCC AGGCCTCGCG TGCAAGTGAG 
ATGGATGCCA CCCGGCCCAA GACAGCCTTC CTGGGTCAGA ACGACGCCTT TGATTTCAGC 
CAGCCGGCAG GTGTCAGTGG CATGGCCTTC CCACGCCCCA AGAGACCTGC CCCGGCCCAA 
GAGGCTGCCA CAGAGGGCCC CAGTGTTGCC TCCAGGGGCA CCCAGGCAGC CTCTGCCGGG 
GAGGGGGCGG CCACCCGGCC CAAGACGACC AAGTCCGGGA AGGCTCTCGC CAAGACTCGC 
TGGGTGGAGC CTCAGAATGT TGTGGCAGCA GCTGCCGCCA AGGCCAAGAT GGCCACGAGC 
ATCCCTGAGC CTGAGAGTGC AGCTGCCACT TCTCAGCAGA GTGCGGAGCC CTGGGCCAGA 
ATGGGAGGCA AGAGGACAAA GAAGTCCAAG CACCTGGATG ACGAATATGA GAGCGGCGAG 
GAGGAGAGAG AGCCTCCTGC GGTCCCACCA ACCTGGAGGG CATCGCAGCC CCTGCTGACG 
ACTGCGCGGC CTCAGGTGGC CCCTCGGCCC TCCATGGCCC TGAGGTCCCA GGTACCCTCA 
AGGCATGTGC TGTGCTTGCC ACCCCGCAAT GTGACCCTTC TGCAAGAGAG GGCAAATAAG 
TTGGTGAAAT ATCTGATGAT TAAAGACTAC AAGAAGATCC CCATCAAGCG CTCAGACATG 
CTGAAGGATG TCATCCGAGA ATATGACGAA CACTTCCCTG AGATCATTGA ACGAGCAACG 
TACACTCTGG AAAAGAAGTT CGGGATCCAC CTGAAGGAAA TTGACAAGGA AGAGCACCTG 
TACATTCTCG TCTGCACACG GGATTCGTCA GCCCGCCTCC TGGGAAAGAC CAAGGATACT 
CCCAGGCTGA GTCTCCTCTT GGTGATTCTG GGTGTCATCT TCATGAACGG CAACCGCGCC 
AGCGAGGCTG TCCTCTGGGA GGCACTCCGC AAGATGGGAC TGCGCCCCGG GGTGAGGCAC 
CCATTCCTTG GCGATCTGAG GAAACTCATT ACAGAGGACT TTGTGAAGCA GAAGTACTTG 
GAATACAAGA AGGTCCCCAA TAGCAGCCCA CCCGAGTATG AGTTTCTCTG GGGTCTGCGC 
GCCTGCCATG AGACCAGCAA GATGAGGGTG CTGAGATTCA TCGCCCAGTA TCAGAACCGA 
GACCCCCGGG AATGGAGGGC TCATTTCTTG GAGGCTGTGG ATGATGCTTT CAAGACGATG 
GATGTGGATA TGGCCGAGGA GCATGCCAGG GCCCAGATGA GGGCCCAGAT GAACATCGGG 
GAGGAAGCTC TGATTGGACG GTGGAGCTGG GACGATATCC AGGTGGAGCT CCTGACCTGG 
GATGAGGACG GAGATTTTGG CGACGCCTGG TCCAGGATCC CCTTCGCTTT CTGGGCCAGA 
TACCATCAGT ACATTCTGAA TAGCAACCGT CCCAACAGGA GAGGTACCTG GAGGGCTGGT 
GTCAGCAGTG GCACCAATGG CGCGGCCAGC GCCAGCATGC TCGATGGCCC CAGCACCAGC 
TCCACCATCC GGACCAGAAA CGCCGCCAGA ACTAGTGCCA GCTTCTTCTC CTGGATTCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED4B ( XM_024987698.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843466.1 Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa238221
Clone ID Related Accession (Same CDS sequence)	XM_024987698.1 , XM_015461704.2 , XM_024987699.1
Accession Version	XM_024987698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	melanoma-associated antigen D4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAGCTACCG CAAGGAATCT GAAGGGTACA ACGTTGAAGA CATGGACGAG 
GGTAGTGATG AAGTCGGGGA GGAAGACATG GTTGAAGGCA ACGACTATGA AGAATTTGGT 
GCTTTCGGAG GCTACGGCGC CCTCACCAGC TTTGACATCC GCATCCTCAG AGCCTTTGGG 
AGCTTGGGTC CAGGCTTTCG CATCTTAGCG AATGAGCCCT GGGAACTGGA AAACCCAGTG 
CTGGCCAGAA CTCTGCTGGA GGCATTTCGG ATGGATCCAG AAACACTTGC CAATGAGACG 
GCTGCCCGTG CTGCCAACGT AGCCCGGGCC GCCGCCTCTA ACCAAGCTGC TCGGGCCGCT 
GCCACTGCTG CCCGTGCCAC CTACAATCAG GTGGTCACTA ACCACCACCC GGTGGCCACA 
CACCAGGCGT CAGGAGGCGA TACCCAGCCC ATGACATCTG CTGCCCAGGC TCCGGCAGCC 
ACCCCTGAGA CAAGCGTCGC TTCTCCGCAC AGCTCCCGGA TGCTAGTCAA TAGCGAGATG 
GCTGCCCCTG GGGCTCCAGC AAGGTCTCCA CAGCCGCAGA CATCCTCCCA GGCCCAGGAG 
GCTGCGGCCG AGGGCCCTAG TACGGCCTGT GCTTTCCCCC AGGCCTCGCG TGCAAGTGAG 
ATGGATGCCA CCCGGCCCAA GACAGCCTTC CTGGGTCAGA ACGACGCCTT TGATTTCAGC 
CAGCCGGCAG GTGTCAGTGG CATGGCCTTC CCACGCCCCA AGAGACCTGC CCCGGCCCAA 
GAGGCTGCCA CAGAGGGCCC CAGTGTTGCC TCCAGGGGCA CCCAGGCAGC CTCTGCCGGG 
GAGGGGGCGG CCACCCGGCC CAAGACGACC AAGTCCGGGA AGGCTCTCGC CAAGACTCGC 
TGGGTGGAGC CTCAGAATGT TGTGGCAGCA GCTGCCGCCA AGGCCAAGAT GGCCACGAGC 
ATCCCTGAGC CTGAGAGTGC AGCTGCCACT TCTCAGCAGA GTGCGGAGCC CTGGGCCAGA 
ATGGGAGGCA AGAGGACAAA GAAGTCCAAG CACCTGGATG ACGAATATGA GAGCGGCGAG 
GAGGAGAGAG AGCCTCCTGC GGTCCCACCA ACCTGGAGGG CATCGCAGCC CCTGCTGACG 
ACTGCGCGGC CTCAGGTGGC CCCTCGGCCC TCCATGGCCC TGAGGTCCCA GGTACCCTCA 
AGGCATGTGC TGTGCTTGCC ACCCCGCAAT GTGACCCTTC TGCAAGAGAG GGCAAATAAG 
TTGGTGAAAT ATCTGATGAT TAAAGACTAC AAGAAGATCC CCATCAAGCG CTCAGACATG 
CTGAAGGATG TCATCCGAGA ATATGACGAA CACTTCCCTG AGATCATTGA ACGAGCAACG 
TACACTCTGG AAAAGAAGTT CGGGATCCAC CTGAAGGAAA TTGACAAGGA AGAGCACCTG 
TACATTCTCG TCTGCACACG GGATTCGTCA GCCCGCCTCC TGGGAAAGAC CAAGGATACT 
CCCAGGCTGA GTCTCCTCTT GGTGATTCTG GGTGTCATCT TCATGAACGG CAACCGCGCC 
AGCGAGGCTG TCCTCTGGGA GGCACTCCGC AAGATGGGAC TGCGCCCCGG GGTGAGGCAC 
CCATTCCTTG GCGATCTGAG GAAACTCATT ACAGAGGACT TTGTGAAGCA GAAGTACTTG 
GAATACAAGA AGGTCCCCAA TAGCAGCCCA CCCGAGTATG AGTTTCTCTG GGGTCTGCGC 
GCCTGCCATG AGACCAGCAA GATGAGGGTG CTGAGATTCA TCGCCCAGTA TCAGAACCGA 
GACCCCCGGG AATGGAGGGC TCATTTCTTG GAGGCTGTGG ATGATGCTTT CAAGACGATG 
GATGTGGATA TGGCCGAGGA GCATGCCAGG GCCCAGATGA GGGCCCAGAT GAACATCGGG 
GAGGAAGCTC TGATTGGACG GTGGAGCTGG GACGATATCC AGGTGGAGCT CCTGACCTGG 
GATGAGGACG GAGATTTTGG CGACGCCTGG TCCAGGATCC CCTTCGCTTT CTGGGCCAGA 
TACCATCAGT ACATTCTGAA TAGCAACCGT GCCAACAGGA GAGGTACCTG GAGGGCTGGT 
GTCAGCAGTG GCACCAATGG CGCGGCCAGC GCCAGCATGC TCGATGGCCC CAGCACCAGC 
TCCACCATCC GGACCAGAAA CGCCGCCAGA ACTAGTGCCA GCTTCTTCTC CTGGATTCAG 
CAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843466.1
CDS	158..2386
Translation	MAEGSYRKESEGYNVEDMDEGSDEVGEEDMVEGNDYEEFGAFGGYGALTSFDIRILRAFGSLGPGFRILANEPWELENPVLARTLLEAFRMDPETLANETAARAANVARAAASNQAARAAATAARATYNQVVTNHHPVATHQASGGDTQPMTSAAQAPAATPETSVASPHSSRMLVNSEMAAPGAPARSPQPQTSSQAQEAAAEGPSTACAFPQASRASEMDATRPKTAFLGQNDAFDFSQPAGVSGMAFPRPKRPAPAQEAATEGPSVASRGTQAASAGEGAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPESAAATSQQSAEPWARMGGKRTKKSKHLDDEYESGEEEREPPAVPPTWRASQPLLTTARPQVAPRPSMALRSQVPSRHVLCLPPRNVTLLQERANKLVKYLMIKDYKKIPIKRSDMLKDVIREYDEHFPEIIERATYTLEKKFGIHLKEIDKEEHLYILVCTRDSSARLLGKTKDTPRLSLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPFLGDLRKLITEDFVKQKYLEYKKVPNSSPPEYEFLWGLRACHETSKMRVLRFIAQYQNRDPREWRAHFLEAVDDAFKTMDVDMAEEHARAQMRAQMNIGEEALIGRWSWDDIQVELLTWDEDGDFGDAWSRIPFAFWARYHQYILNSNRANRRGTWRAGVSSGTNGAASASMLDGPSTSSTIRTRNAARTSASFFSWIQQP

Target ORF information:

RefSeq Version	XM_024987698.1
Organism	Bos taurus(cattle)
Definition	Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987698.1

ORF Insert Sequence:

ATGGCAGAGG GAAGCTACCG CAAGGAATCT GAAGGGTACA ACGTTGAAGA CATGGACGAG 
GGTAGTGATG AAGTCGGGGA GGAAGACATG GTTGAAGGCA ACGACTATGA AGAATTTGGT 
GCTTTCGGAG GCTACGGCGC CCTCACCAGC TTTGACATCC GCATCCTCAG AGCCTTTGGG 
AGCTTGGGTC CAGGCTTTCG CATCTTAGCG AATGAGCCCT GGGAACTGGA AAACCCAGTG 
CTGGCCAGAA CTCTGCTGGA GGCATTTCGG ATGGATCCAG AAACACTTGC CAATGAGACG 
GCTGCCCGTG CTGCCAACGT AGCCCGGGCC GCCGCCTCTA ACCAAGCTGC TCGGGCCGCT 
GCCACTGCTG CCCGTGCCAC CTACAATCAG GTGGTCACTA ACCACCACCC GGTGGCCACA 
CACCAGGCGT CAGGAGGCGA TACCCAGCCC ATGACATCTG CTGCCCAGGC TCCGGCAGCC 
ACCCCTGAGA CAAGCGTCGC TTCTCCGCAC AGCTCCCGGA TGCTAGTCAA TAGCGAGATG 
GCTGCCCCTG GGGCTCCAGC AAGGTCTCCA CAGCCGCAGA CATCCTCCCA GGCCCAGGAG 
GCTGCGGCCG AGGGCCCTAG TACGGCCTGT GCTTTCCCCC AGGCCTCGCG TGCAAGTGAG 
ATGGATGCCA CCCGGCCCAA GACAGCCTTC CTGGGTCAGA ACGACGCCTT TGATTTCAGC 
CAGCCGGCAG GTGTCAGTGG CATGGCCTTC CCACGCCCCA AGAGACCTGC CCCGGCCCAA 
GAGGCTGCCA CAGAGGGCCC CAGTGTTGCC TCCAGGGGCA CCCAGGCAGC CTCTGCCGGG 
GAGGGGGCGG CCACCCGGCC CAAGACGACC AAGTCCGGGA AGGCTCTCGC CAAGACTCGC 
TGGGTGGAGC CTCAGAATGT TGTGGCAGCA GCTGCCGCCA AGGCCAAGAT GGCCACGAGC 
ATCCCTGAGC CTGAGAGTGC AGCTGCCACT TCTCAGCAGA GTGCGGAGCC CTGGGCCAGA 
ATGGGAGGCA AGAGGACAAA GAAGTCCAAG CACCTGGATG ACGAATATGA GAGCGGCGAG 
GAGGAGAGAG AGCCTCCTGC GGTCCCACCA ACCTGGAGGG CATCGCAGCC CCTGCTGACG 
ACTGCGCGGC CTCAGGTGGC CCCTCGGCCC TCCATGGCCC TGAGGTCCCA GGTACCCTCA 
AGGCATGTGC TGTGCTTGCC ACCCCGCAAT GTGACCCTTC TGCAAGAGAG GGCAAATAAG 
TTGGTGAAAT ATCTGATGAT TAAAGACTAC AAGAAGATCC CCATCAAGCG CTCAGACATG 
CTGAAGGATG TCATCCGAGA ATATGACGAA CACTTCCCTG AGATCATTGA ACGAGCAACG 
TACACTCTGG AAAAGAAGTT CGGGATCCAC CTGAAGGAAA TTGACAAGGA AGAGCACCTG 
TACATTCTCG TCTGCACACG GGATTCGTCA GCCCGCCTCC TGGGAAAGAC CAAGGATACT 
CCCAGGCTGA GTCTCCTCTT GGTGATTCTG GGTGTCATCT TCATGAACGG CAACCGCGCC 
AGCGAGGCTG TCCTCTGGGA GGCACTCCGC AAGATGGGAC TGCGCCCCGG GGTGAGGCAC 
CCATTCCTTG GCGATCTGAG GAAACTCATT ACAGAGGACT TTGTGAAGCA GAAGTACTTG 
GAATACAAGA AGGTCCCCAA TAGCAGCCCA CCCGAGTATG AGTTTCTCTG GGGTCTGCGC 
GCCTGCCATG AGACCAGCAA GATGAGGGTG CTGAGATTCA TCGCCCAGTA TCAGAACCGA 
GACCCCCGGG AATGGAGGGC TCATTTCTTG GAGGCTGTGG ATGATGCTTT CAAGACGATG 
GATGTGGATA TGGCCGAGGA GCATGCCAGG GCCCAGATGA GGGCCCAGAT GAACATCGGG 
GAGGAAGCTC TGATTGGACG GTGGAGCTGG GACGATATCC AGGTGGAGCT CCTGACCTGG 
GATGAGGACG GAGATTTTGG CGACGCCTGG TCCAGGATCC CCTTCGCTTT CTGGGCCAGA 
TACCATCAGT ACATTCTGAA TAGCAACCGT GCCAACAGGA GAGGTACCTG GAGGGCTGGT 
GTCAGCAGTG GCACCAATGG CGCGGCCAGC GCCAGCATGC TCGATGGCCC CAGCACCAGC 
TCCACCATCC GGACCAGAAA CGCCGCCAGA ACTAGTGCCA GCTTCTTCTC CTGGATTCAG 
CAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED4B ( XM_015461704.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317190.2 Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X1, mRNA.

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CloneID	OBa238221
Clone ID Related Accession (Same CDS sequence)	XM_024987698.1 , XM_015461704.2 , XM_024987699.1
Accession Version	XM_015461704.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	melanoma-associated antigen D4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461704.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAGCTACCG CAAGGAATCT GAAGGGTACA ACGTTGAAGA CATGGACGAG 
GGTAGTGATG AAGTCGGGGA GGAAGACATG GTTGAAGGCA ACGACTATGA AGAATTTGGT 
GCTTTCGGAG GCTACGGCGC CCTCACCAGC TTTGACATCC GCATCCTCAG AGCCTTTGGG 
AGCTTGGGTC CAGGCTTTCG CATCTTAGCG AATGAGCCCT GGGAACTGGA AAACCCAGTG 
CTGGCCAGAA CTCTGCTGGA GGCATTTCGG ATGGATCCAG AAACACTTGC CAATGAGACG 
GCTGCCCGTG CTGCCAACGT AGCCCGGGCC GCCGCCTCTA ACCAAGCTGC TCGGGCCGCT 
GCCACTGCTG CCCGTGCCAC CTACAATCAG GTGGTCACTA ACCACCACCC GGTGGCCACA 
CACCAGGCGT CAGGAGGCGA TACCCAGCCC ATGACATCTG CTGCCCAGGC TCCGGCAGCC 
ACCCCTGAGA CAAGCGTCGC TTCTCCGCAC AGCTCCCGGA TGCTAGTCAA TAGCGAGATG 
GCTGCCCCTG GGGCTCCAGC AAGGTCTCCA CAGCCGCAGA CATCCTCCCA GGCCCAGGAG 
GCTGCGGCCG AGGGCCCTAG TACGGCCTGT GCTTTCCCCC AGGCCTCGCG TGCAAGTGAG 
ATGGATGCCA CCCGGCCCAA GACAGCCTTC CTGGGTCAGA ACGACGCCTT TGATTTCAGC 
CAGCCGGCAG GTGTCAGTGG CATGGCCTTC CCACGCCCCA AGAGACCTGC CCCGGCCCAA 
GAGGCTGCCA CAGAGGGCCC CAGTGTTGCC TCCAGGGGCA CCCAGGCAGC CTCTGCCGGG 
GAGGGGGCGG CCACCCGGCC CAAGACGACC AAGTCCGGGA AGGCTCTCGC CAAGACTCGC 
TGGGTGGAGC CTCAGAATGT TGTGGCAGCA GCTGCCGCCA AGGCCAAGAT GGCCACGAGC 
ATCCCTGAGC CTGAGAGTGC AGCTGCCACT TCTCAGCAGA GTGCGGAGCC CTGGGCCAGA 
ATGGGAGGCA AGAGGACAAA GAAGTCCAAG CACCTGGATG ACGAATATGA GAGCGGCGAG 
GAGGAGAGAG AGCCTCCTGC GGTCCCACCA ACCTGGAGGG CATCGCAGCC CCTGCTGACG 
ACTGCGCGGC CTCAGGTGGC CCCTCGGCCC TCCATGGCCC TGAGGTCCCA GGTACCCTCA 
AGGCATGTGC TGTGCTTGCC ACCCCGCAAT GTGACCCTTC TGCAAGAGAG GGCAAATAAG 
TTGGTGAAAT ATCTGATGAT TAAAGACTAC AAGAAGATCC CCATCAAGCG CTCAGACATG 
CTGAAGGATG TCATCCGAGA ATATGACGAA CACTTCCCTG AGATCATTGA ACGAGCAACG 
TACACTCTGG AAAAGAAGTT CGGGATCCAC CTGAAGGAAA TTGACAAGGA AGAGCACCTG 
TACATTCTCG TCTGCACACG GGATTCGTCA GCCCGCCTCC TGGGAAAGAC CAAGGATACT 
CCCAGGCTGA GTCTCCTCTT GGTGATTCTG GGTGTCATCT TCATGAACGG CAACCGCGCC 
AGCGAGGCTG TCCTCTGGGA GGCACTCCGC AAGATGGGAC TGCGCCCCGG GGTGAGGCAC 
CCATTCCTTG GCGATCTGAG GAAACTCATT ACAGAGGACT TTGTGAAGCA GAAGTACTTG 
GAATACAAGA AGGTCCCCAA TAGCAGCCCA CCCGAGTATG AGTTTCTCTG GGGTCTGCGC 
GCCTGCCATG AGACCAGCAA GATGAGGGTG CTGAGATTCA TCGCCCAGTA TCAGAACCGA 
GACCCCCGGG AATGGAGGGC TCATTTCTTG GAGGCTGTGG ATGATGCTTT CAAGACGATG 
GATGTGGATA TGGCCGAGGA GCATGCCAGG GCCCAGATGA GGGCCCAGAT GAACATCGGG 
GAGGAAGCTC TGATTGGACG GTGGAGCTGG GACGATATCC AGGTGGAGCT CCTGACCTGG 
GATGAGGACG GAGATTTTGG CGACGCCTGG TCCAGGATCC CCTTCGCTTT CTGGGCCAGA 
TACCATCAGT ACATTCTGAA TAGCAACCGT GCCAACAGGA GAGGTACCTG GAGGGCTGGT 
GTCAGCAGTG GCACCAATGG CGCGGCCAGC GCCAGCATGC TCGATGGCCC CAGCACCAGC 
TCCACCATCC GGACCAGAAA CGCCGCCAGA ACTAGTGCCA GCTTCTTCTC CTGGATTCAG 
CAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317190.2
CDS	136..2364
Translation	MAEGSYRKESEGYNVEDMDEGSDEVGEEDMVEGNDYEEFGAFGGYGALTSFDIRILRAFGSLGPGFRILANEPWELENPVLARTLLEAFRMDPETLANETAARAANVARAAASNQAARAAATAARATYNQVVTNHHPVATHQASGGDTQPMTSAAQAPAATPETSVASPHSSRMLVNSEMAAPGAPARSPQPQTSSQAQEAAAEGPSTACAFPQASRASEMDATRPKTAFLGQNDAFDFSQPAGVSGMAFPRPKRPAPAQEAATEGPSVASRGTQAASAGEGAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPESAAATSQQSAEPWARMGGKRTKKSKHLDDEYESGEEEREPPAVPPTWRASQPLLTTARPQVAPRPSMALRSQVPSRHVLCLPPRNVTLLQERANKLVKYLMIKDYKKIPIKRSDMLKDVIREYDEHFPEIIERATYTLEKKFGIHLKEIDKEEHLYILVCTRDSSARLLGKTKDTPRLSLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPFLGDLRKLITEDFVKQKYLEYKKVPNSSPPEYEFLWGLRACHETSKMRVLRFIAQYQNRDPREWRAHFLEAVDDAFKTMDVDMAEEHARAQMRAQMNIGEEALIGRWSWDDIQVELLTWDEDGDFGDAWSRIPFAFWARYHQYILNSNRANRRGTWRAGVSSGTNGAASASMLDGPSTSSTIRTRNAARTSASFFSWIQQP

Target ORF information:

RefSeq Version	XM_015461704.2
Organism	Bos taurus(cattle)
Definition	Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461704.2

ORF Insert Sequence:

ATGGCAGAGG GAAGCTACCG CAAGGAATCT GAAGGGTACA ACGTTGAAGA CATGGACGAG 
GGTAGTGATG AAGTCGGGGA GGAAGACATG GTTGAAGGCA ACGACTATGA AGAATTTGGT 
GCTTTCGGAG GCTACGGCGC CCTCACCAGC TTTGACATCC GCATCCTCAG AGCCTTTGGG 
AGCTTGGGTC CAGGCTTTCG CATCTTAGCG AATGAGCCCT GGGAACTGGA AAACCCAGTG 
CTGGCCAGAA CTCTGCTGGA GGCATTTCGG ATGGATCCAG AAACACTTGC CAATGAGACG 
GCTGCCCGTG CTGCCAACGT AGCCCGGGCC GCCGCCTCTA ACCAAGCTGC TCGGGCCGCT 
GCCACTGCTG CCCGTGCCAC CTACAATCAG GTGGTCACTA ACCACCACCC GGTGGCCACA 
CACCAGGCGT CAGGAGGCGA TACCCAGCCC ATGACATCTG CTGCCCAGGC TCCGGCAGCC 
ACCCCTGAGA CAAGCGTCGC TTCTCCGCAC AGCTCCCGGA TGCTAGTCAA TAGCGAGATG 
GCTGCCCCTG GGGCTCCAGC AAGGTCTCCA CAGCCGCAGA CATCCTCCCA GGCCCAGGAG 
GCTGCGGCCG AGGGCCCTAG TACGGCCTGT GCTTTCCCCC AGGCCTCGCG TGCAAGTGAG 
ATGGATGCCA CCCGGCCCAA GACAGCCTTC CTGGGTCAGA ACGACGCCTT TGATTTCAGC 
CAGCCGGCAG GTGTCAGTGG CATGGCCTTC CCACGCCCCA AGAGACCTGC CCCGGCCCAA 
GAGGCTGCCA CAGAGGGCCC CAGTGTTGCC TCCAGGGGCA CCCAGGCAGC CTCTGCCGGG 
GAGGGGGCGG CCACCCGGCC CAAGACGACC AAGTCCGGGA AGGCTCTCGC CAAGACTCGC 
TGGGTGGAGC CTCAGAATGT TGTGGCAGCA GCTGCCGCCA AGGCCAAGAT GGCCACGAGC 
ATCCCTGAGC CTGAGAGTGC AGCTGCCACT TCTCAGCAGA GTGCGGAGCC CTGGGCCAGA 
ATGGGAGGCA AGAGGACAAA GAAGTCCAAG CACCTGGATG ACGAATATGA GAGCGGCGAG 
GAGGAGAGAG AGCCTCCTGC GGTCCCACCA ACCTGGAGGG CATCGCAGCC CCTGCTGACG 
ACTGCGCGGC CTCAGGTGGC CCCTCGGCCC TCCATGGCCC TGAGGTCCCA GGTACCCTCA 
AGGCATGTGC TGTGCTTGCC ACCCCGCAAT GTGACCCTTC TGCAAGAGAG GGCAAATAAG 
TTGGTGAAAT ATCTGATGAT TAAAGACTAC AAGAAGATCC CCATCAAGCG CTCAGACATG 
CTGAAGGATG TCATCCGAGA ATATGACGAA CACTTCCCTG AGATCATTGA ACGAGCAACG 
TACACTCTGG AAAAGAAGTT CGGGATCCAC CTGAAGGAAA TTGACAAGGA AGAGCACCTG 
TACATTCTCG TCTGCACACG GGATTCGTCA GCCCGCCTCC TGGGAAAGAC CAAGGATACT 
CCCAGGCTGA GTCTCCTCTT GGTGATTCTG GGTGTCATCT TCATGAACGG CAACCGCGCC 
AGCGAGGCTG TCCTCTGGGA GGCACTCCGC AAGATGGGAC TGCGCCCCGG GGTGAGGCAC 
CCATTCCTTG GCGATCTGAG GAAACTCATT ACAGAGGACT TTGTGAAGCA GAAGTACTTG 
GAATACAAGA AGGTCCCCAA TAGCAGCCCA CCCGAGTATG AGTTTCTCTG GGGTCTGCGC 
GCCTGCCATG AGACCAGCAA GATGAGGGTG CTGAGATTCA TCGCCCAGTA TCAGAACCGA 
GACCCCCGGG AATGGAGGGC TCATTTCTTG GAGGCTGTGG ATGATGCTTT CAAGACGATG 
GATGTGGATA TGGCCGAGGA GCATGCCAGG GCCCAGATGA GGGCCCAGAT GAACATCGGG 
GAGGAAGCTC TGATTGGACG GTGGAGCTGG GACGATATCC AGGTGGAGCT CCTGACCTGG 
GATGAGGACG GAGATTTTGG CGACGCCTGG TCCAGGATCC CCTTCGCTTT CTGGGCCAGA 
TACCATCAGT ACATTCTGAA TAGCAACCGT GCCAACAGGA GAGGTACCTG GAGGGCTGGT 
GTCAGCAGTG GCACCAATGG CGCGGCCAGC GCCAGCATGC TCGATGGCCC CAGCACCAGC 
TCCACCATCC GGACCAGAAA CGCCGCCAGA ACTAGTGCCA GCTTCTTCTC CTGGATTCAG 
CAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED4B ( XM_024987699.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843467.1 Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X3, mRNA.

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CloneID	OBa238221
Clone ID Related Accession (Same CDS sequence)	XM_024987698.1 , XM_015461704.2 , XM_024987699.1
Accession Version	XM_024987699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	melanoma-associated antigen D4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAGCTACCG CAAGGAATCT GAAGGGTACA ACGTTGAAGA CATGGACGAG 
GGTAGTGATG AAGTCGGGGA GGAAGACATG GTTGAAGGCA ACGACTATGA AGAATTTGGT 
GCTTTCGGAG GCTACGGCGC CCTCACCAGC TTTGACATCC GCATCCTCAG AGCCTTTGGG 
AGCTTGGGTC CAGGCTTTCG CATCTTAGCG AATGAGCCCT GGGAACTGGA AAACCCAGTG 
CTGGCCAGAA CTCTGCTGGA GGCATTTCGG ATGGATCCAG AAACACTTGC CAATGAGACG 
GCTGCCCGTG CTGCCAACGT AGCCCGGGCC GCCGCCTCTA ACCAAGCTGC TCGGGCCGCT 
GCCACTGCTG CCCGTGCCAC CTACAATCAG GTGGTCACTA ACCACCACCC GGTGGCCACA 
CACCAGGCGT CAGGAGGCGA TACCCAGCCC ATGACATCTG CTGCCCAGGC TCCGGCAGCC 
ACCCCTGAGA CAAGCGTCGC TTCTCCGCAC AGCTCCCGGA TGCTAGTCAA TAGCGAGATG 
GCTGCCCCTG GGGCTCCAGC AAGGTCTCCA CAGCCGCAGA CATCCTCCCA GGCCCAGGAG 
GCTGCGGCCG AGGGCCCTAG TACGGCCTGT GCTTTCCCCC AGGCCTCGCG TGCAAGTGAG 
ATGGATGCCA CCCGGCCCAA GACAGCCTTC CTGGGTCAGA ACGACGCCTT TGATTTCAGC 
CAGCCGGCAG GTGTCAGTGG CATGGCCTTC CCACGCCCCA AGAGACCTGC CCCGGCCCAA 
GAGGCTGCCA CAGAGGGCCC CAGTGTTGCC TCCAGGGGCA CCCAGGCAGC CTCTGCCGGG 
GAGGGGGCGG CCACCCGGCC CAAGACGACC AAGTCCGGGA AGGCTCTCGC CAAGACTCGC 
TGGGTGGAGC CTCAGAATGT TGTGGCAGCA GCTGCCGCCA AGGCCAAGAT GGCCACGAGC 
ATCCCTGAGC CTGAGAGTGC AGCTGCCACT TCTCAGCAGA GTGCGGAGCC CTGGGCCAGA 
ATGGGAGGCA AGAGGACAAA GAAGTCCAAG CACCTGGATG ACGAATATGA GAGCGGCGAG 
GAGGAGAGAG AGCCTCCTGC GGTCCCACCA ACCTGGAGGG CATCGCAGCC CCTGCTGACG 
ACTGCGCGGC CTCAGGTGGC CCCTCGGCCC TCCATGGCCC TGAGGTCCCA GGTACCCTCA 
AGGCATGTGC TGTGCTTGCC ACCCCGCAAT GTGACCCTTC TGCAAGAGAG GGCAAATAAG 
TTGGTGAAAT ATCTGATGAT TAAAGACTAC AAGAAGATCC CCATCAAGCG CTCAGACATG 
CTGAAGGATG TCATCCGAGA ATATGACGAA CACTTCCCTG AGATCATTGA ACGAGCAACG 
TACACTCTGG AAAAGAAGTT CGGGATCCAC CTGAAGGAAA TTGACAAGGA AGAGCACCTG 
TACATTCTCG TCTGCACACG GGATTCGTCA GCCCGCCTCC TGGGAAAGAC CAAGGATACT 
CCCAGGCTGA GTCTCCTCTT GGTGATTCTG GGTGTCATCT TCATGAACGG CAACCGCGCC 
AGCGAGGCTG TCCTCTGGGA GGCACTCCGC AAGATGGGAC TGCGCCCCGG GGTGAGGCAC 
CCATTCCTTG GCGATCTGAG GAAACTCATT ACAGAGGACT TTGTGAAGCA GAAGTACTTG 
GAATACAAGA AGGTCCCCAA TAGCAGCCCA CCCGAGTATG AGTTTCTCTG GGGTCTGCGC 
GCCTGCCATG AGACCAGCAA GATGAGGGTG CTGAGATTCA TCGCCCAGTA TCAGAACCGA 
GACCCCCGGG AATGGAGGGC TCATTTCTTG GAGGCTGTGG ATGATGCTTT CAAGACGATG 
GATGTGGATA TGGCCGAGGA GCATGCCAGG GCCCAGATGA GGGCCCAGAT GAACATCGGG 
GAGGAAGCTC TGATTGGACG GTGGAGCTGG GACGATATCC AGGTGGAGCT CCTGACCTGG 
GATGAGGACG GAGATTTTGG CGACGCCTGG TCCAGGATCC CCTTCGCTTT CTGGGCCAGA 
TACCATCAGT ACATTCTGAA TAGCAACCGT GCCAACAGGA GAGGTACCTG GAGGGCTGGT 
GTCAGCAGTG GCACCAATGG CGCGGCCAGC GCCAGCATGC TCGATGGCCC CAGCACCAGC 
TCCACCATCC GGACCAGAAA CGCCGCCAGA ACTAGTGCCA GCTTCTTCTC CTGGATTCAG 
CAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843467.1
CDS	295..2523
Translation	MAEGSYRKESEGYNVEDMDEGSDEVGEEDMVEGNDYEEFGAFGGYGALTSFDIRILRAFGSLGPGFRILANEPWELENPVLARTLLEAFRMDPETLANETAARAANVARAAASNQAARAAATAARATYNQVVTNHHPVATHQASGGDTQPMTSAAQAPAATPETSVASPHSSRMLVNSEMAAPGAPARSPQPQTSSQAQEAAAEGPSTACAFPQASRASEMDATRPKTAFLGQNDAFDFSQPAGVSGMAFPRPKRPAPAQEAATEGPSVASRGTQAASAGEGAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPESAAATSQQSAEPWARMGGKRTKKSKHLDDEYESGEEEREPPAVPPTWRASQPLLTTARPQVAPRPSMALRSQVPSRHVLCLPPRNVTLLQERANKLVKYLMIKDYKKIPIKRSDMLKDVIREYDEHFPEIIERATYTLEKKFGIHLKEIDKEEHLYILVCTRDSSARLLGKTKDTPRLSLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPFLGDLRKLITEDFVKQKYLEYKKVPNSSPPEYEFLWGLRACHETSKMRVLRFIAQYQNRDPREWRAHFLEAVDDAFKTMDVDMAEEHARAQMRAQMNIGEEALIGRWSWDDIQVELLTWDEDGDFGDAWSRIPFAFWARYHQYILNSNRANRRGTWRAGVSSGTNGAASASMLDGPSTSSTIRTRNAARTSASFFSWIQQP

Target ORF information:

RefSeq Version	XM_024987699.1
Organism	Bos taurus(cattle)
Definition	Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987699.1

ORF Insert Sequence:

ATGGCAGAGG GAAGCTACCG CAAGGAATCT GAAGGGTACA ACGTTGAAGA CATGGACGAG 
GGTAGTGATG AAGTCGGGGA GGAAGACATG GTTGAAGGCA ACGACTATGA AGAATTTGGT 
GCTTTCGGAG GCTACGGCGC CCTCACCAGC TTTGACATCC GCATCCTCAG AGCCTTTGGG 
AGCTTGGGTC CAGGCTTTCG CATCTTAGCG AATGAGCCCT GGGAACTGGA AAACCCAGTG 
CTGGCCAGAA CTCTGCTGGA GGCATTTCGG ATGGATCCAG AAACACTTGC CAATGAGACG 
GCTGCCCGTG CTGCCAACGT AGCCCGGGCC GCCGCCTCTA ACCAAGCTGC TCGGGCCGCT 
GCCACTGCTG CCCGTGCCAC CTACAATCAG GTGGTCACTA ACCACCACCC GGTGGCCACA 
CACCAGGCGT CAGGAGGCGA TACCCAGCCC ATGACATCTG CTGCCCAGGC TCCGGCAGCC 
ACCCCTGAGA CAAGCGTCGC TTCTCCGCAC AGCTCCCGGA TGCTAGTCAA TAGCGAGATG 
GCTGCCCCTG GGGCTCCAGC AAGGTCTCCA CAGCCGCAGA CATCCTCCCA GGCCCAGGAG 
GCTGCGGCCG AGGGCCCTAG TACGGCCTGT GCTTTCCCCC AGGCCTCGCG TGCAAGTGAG 
ATGGATGCCA CCCGGCCCAA GACAGCCTTC CTGGGTCAGA ACGACGCCTT TGATTTCAGC 
CAGCCGGCAG GTGTCAGTGG CATGGCCTTC CCACGCCCCA AGAGACCTGC CCCGGCCCAA 
GAGGCTGCCA CAGAGGGCCC CAGTGTTGCC TCCAGGGGCA CCCAGGCAGC CTCTGCCGGG 
GAGGGGGCGG CCACCCGGCC CAAGACGACC AAGTCCGGGA AGGCTCTCGC CAAGACTCGC 
TGGGTGGAGC CTCAGAATGT TGTGGCAGCA GCTGCCGCCA AGGCCAAGAT GGCCACGAGC 
ATCCCTGAGC CTGAGAGTGC AGCTGCCACT TCTCAGCAGA GTGCGGAGCC CTGGGCCAGA 
ATGGGAGGCA AGAGGACAAA GAAGTCCAAG CACCTGGATG ACGAATATGA GAGCGGCGAG 
GAGGAGAGAG AGCCTCCTGC GGTCCCACCA ACCTGGAGGG CATCGCAGCC CCTGCTGACG 
ACTGCGCGGC CTCAGGTGGC CCCTCGGCCC TCCATGGCCC TGAGGTCCCA GGTACCCTCA 
AGGCATGTGC TGTGCTTGCC ACCCCGCAAT GTGACCCTTC TGCAAGAGAG GGCAAATAAG 
TTGGTGAAAT ATCTGATGAT TAAAGACTAC AAGAAGATCC CCATCAAGCG CTCAGACATG 
CTGAAGGATG TCATCCGAGA ATATGACGAA CACTTCCCTG AGATCATTGA ACGAGCAACG 
TACACTCTGG AAAAGAAGTT CGGGATCCAC CTGAAGGAAA TTGACAAGGA AGAGCACCTG 
TACATTCTCG TCTGCACACG GGATTCGTCA GCCCGCCTCC TGGGAAAGAC CAAGGATACT 
CCCAGGCTGA GTCTCCTCTT GGTGATTCTG GGTGTCATCT TCATGAACGG CAACCGCGCC 
AGCGAGGCTG TCCTCTGGGA GGCACTCCGC AAGATGGGAC TGCGCCCCGG GGTGAGGCAC 
CCATTCCTTG GCGATCTGAG GAAACTCATT ACAGAGGACT TTGTGAAGCA GAAGTACTTG 
GAATACAAGA AGGTCCCCAA TAGCAGCCCA CCCGAGTATG AGTTTCTCTG GGGTCTGCGC 
GCCTGCCATG AGACCAGCAA GATGAGGGTG CTGAGATTCA TCGCCCAGTA TCAGAACCGA 
GACCCCCGGG AATGGAGGGC TCATTTCTTG GAGGCTGTGG ATGATGCTTT CAAGACGATG 
GATGTGGATA TGGCCGAGGA GCATGCCAGG GCCCAGATGA GGGCCCAGAT GAACATCGGG 
GAGGAAGCTC TGATTGGACG GTGGAGCTGG GACGATATCC AGGTGGAGCT CCTGACCTGG 
GATGAGGACG GAGATTTTGG CGACGCCTGG TCCAGGATCC CCTTCGCTTT CTGGGCCAGA 
TACCATCAGT ACATTCTGAA TAGCAACCGT GCCAACAGGA GAGGTACCTG GAGGGCTGGT 
GTCAGCAGTG GCACCAATGG CGCGGCCAGC GCCAGCATGC TCGATGGCCC CAGCACCAGC 
TCCACCATCC GGACCAGAAA CGCCGCCAGA ACTAGTGCCA GCTTCTTCTC CTGGATTCAG 
CAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED4B ( XM_024987700.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843468.1 Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X4, mRNA.

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CloneID	OBa238222
Clone ID Related Accession (Same CDS sequence)	XM_024987700.1 , XM_024987701.1
Accession Version	XM_024987700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	melanoma-associated antigen D4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAGCTACCG CAAGGAATCT GAAGGGTACA ACGTTGAAGA CATGGACGAG 
GGTAGTGATG AAGTCGGGGA GGAAGACATG GTTGAAGGCA ACGACTATGA AGAATTTGGT 
GCTTTCGGAG GCTACGGCGC CCTCACCAGC TTTGACATCC GCATCCTCAG AGCCTTTGGG 
AGCTTGGGTC CAGGCTTTCG CATCTTAGCG AATGAGCCCT GGGAACTGGA AAACCCAGTG 
CTGGCCAGAA CTCTGCTGGA GGCATTTCGG ATGGATCCAG AAACACTTGC CAATGAGACG 
GCTGCCCGTG CTGCCAACGT AGCCCGGGCC GCCGCCTCTA ACCAAGCTGC TCGGGCCGCT 
GCCACTGCTG CCCGTGCCAC CTACAATCAG GTGGTCACTA ACCACCACCC GGTGGCCACA 
CACCAGGCGT CAGGAGGCGA TACCCAGCCC ATGACATCTG CTGCCCAGGC TCCGGCAGCC 
ACCCCTGAGA CAAGCGTCGC TTCTCCGCAC AGCTCCCGGA TGCTAGTCAA TAGCGAGATG 
GCTGCCCCTG GGGCTCCAGC AAGGTCTCCA CAGCCGCAGA CATCCTCCCA GGCCCAGGAG 
GCTGCGGCCG AGGGCCCTAG TACGGCCTGT GCTTTCCCCC AGGCCTCGCG TGCAAGTGAG 
ATGGATGCCA CCCGGCCCAA GACAGCCTTC CTGGGTCAGA ACGACGCCTT TGATTTCAGC 
CAGCCGGCAG GTGTCAGTGG CATGGCCTTC CCACGCCCCA AGAGACCTGC CCCGGCCCAA 
GAGGCTGCCA CAGAGGGCCC CAGTGTTGCC TCCAGGGGCA CCCAGGCAGC CTCTGCCGGG 
GAGGGGGCGG CCACCCGGCC CAAGACGACC AAGTCCGGGA AGGCTCTCGC CAAGACTCGC 
TGGGTGGAGC CTCAGAATGT TGTGGCAGCA GCTGCCGCCA AGGCCAAGAT GGCCACGAGC 
ATCCCTGAGC CTGAGAGTGC AGCTGCCACT TCTCAGCAGA GTGCGGAGCC CTGGGCCAGA 
ATGGGAGGCA AGAGGACAAA GAAGTCCAAG CACCTGGATG ACGAATATGA GAGCGGCGAG 
GAGGAGAGAG AGCCTCCTGC GGTCCCACCA ACCTGGAGGG CATCGCAGCC CCTGCTGACG 
ACTGCGCGGC CTCAGGTGGC CCCTCGGCCC TCCATGGCCC TGAGGTCCCA GGTACCCTCA 
AGGCATGTGC TGTGCTTGCC ACCCCGCAAT GTGACCCTTC TGCAAGAGAG GGCAAATAAG 
TTGGTGAAAT ATCTGATGAT TAAAGACTAC AAGAAGATCC CCATCAAGCG CTCAGACATG 
CTGAAGGATG TCATCCGAGA ATATGACGAA CACTTCCCTG AGATCATTGA ACGAGCAACG 
TACACTCTGG AAAAGAAGTT CGGGATCCAC CTGAAGGAAA TTGACAAGGA AGAGCACCTG 
TACATTCTCG TCTGCACACG GGATTCGTCA GCCCGCCTCC TGGGAAAGAC CAAGGATACT 
CCCAGGCTGA GTCTCCTCTT GGTGATTCTG GGTGTCATCT TCATGAACGG CAACCGCGCC 
AGCGAGGCTG TCCTCTGGGA GGCACTCCGC AAGATGGGAC TGCGCCCCGG GGTGAGGCAC 
CCATTCCTTG GCGATCTGAG GAAACTCATT ACAGAGGACT TTGTGAAGCA GAAGTACTTG 
GAATACAAGA AGGTCCCCAA TAGCAGCCCA CCCGAGTATG AGTTTCTCTG GGGTCTGCGC 
GCCTGCCATG AGACCAGCAA GATGAGGGTG CTGAGATTCA TCGCCCAGTA TCAGAACCGA 
GACCCCCGGG AATGGAGGGC TCATTTCTTG GAGGCTGTGG ATGATGCTTT CAAGACGATG 
GATGTGGATA TGGCCGAGGA GCATGCCAGG GCCCAGATGA GGGCCCAGAT GAACATCGGG 
GAGGAAGCTC TGATTGGACG GTGGAGCTGG GACGATATCC AGGTGGAGCT CCTGACCTGG 
GATGAGGACG GAGATTTTGG CGACGCCTGG TCCAGGATCC CCTTCGCTTT CTGGGCCAGA 
TACCATCAGT ACATTCTGAA TAGCAACCGT GCCAACAGGA GAGGTACCTG GAGGGCTGGT 
GTCAGCAGTG GCACCAATGG CGCGGCCAGC GCCAGCATGC TCGATGGCCC CAGCACCAGC 
TCCACCATCC GGACCAGAAA CGCCGCCAGA ACTAGTGCCA GCTTCTTCTC CTGGATTCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843468.1
CDS	127..2349
Translation	MAEGSYRKESEGYNVEDMDEGSDEVGEEDMVEGNDYEEFGAFGGYGALTSFDIRILRAFGSLGPGFRILANEPWELENPVLARTLLEAFRMDPETLANETAARAANVARAAASNQAARAAATAARATYNQVVTNHHPVATHQASGGDTQPMTSAAQAPAATPETSVASPHSSRMLVNSEMAAPGAPARSPQPQTSSQAQEAAAEGPSTACAFPQASRASEMDATRPKTAFLGQNDAFDFSQPAGVSGMAFPRPKRPAPAQEAATEGPSVASRGTQAASAGEGAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPESAAATSQQSAEPWARMGGKRTKKSKHLDDEYESGEEEREPPAVPPTWRASQPLLTTARPQVAPRPSMALRSQVPSRHVLCLPPRNVTLLQERANKLVKYLMIKDYKKIPIKRSDMLKDVIREYDEHFPEIIERATYTLEKKFGIHLKEIDKEEHLYILVCTRDSSARLLGKTKDTPRLSLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPFLGDLRKLITEDFVKQKYLEYKKVPNSSPPEYEFLWGLRACHETSKMRVLRFIAQYQNRDPREWRAHFLEAVDDAFKTMDVDMAEEHARAQMRAQMNIGEEALIGRWSWDDIQVELLTWDEDGDFGDAWSRIPFAFWARYHQYILNSNRANRRGTWRAGVSSGTNGAASASMLDGPSTSSTIRTRNAARTSASFFSWIQ

Target ORF information:

RefSeq Version	XM_024987700.1
Organism	Bos taurus(cattle)
Definition	Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987700.1

ORF Insert Sequence:

ATGGCAGAGG GAAGCTACCG CAAGGAATCT GAAGGGTACA ACGTTGAAGA CATGGACGAG 
GGTAGTGATG AAGTCGGGGA GGAAGACATG GTTGAAGGCA ACGACTATGA AGAATTTGGT 
GCTTTCGGAG GCTACGGCGC CCTCACCAGC TTTGACATCC GCATCCTCAG AGCCTTTGGG 
AGCTTGGGTC CAGGCTTTCG CATCTTAGCG AATGAGCCCT GGGAACTGGA AAACCCAGTG 
CTGGCCAGAA CTCTGCTGGA GGCATTTCGG ATGGATCCAG AAACACTTGC CAATGAGACG 
GCTGCCCGTG CTGCCAACGT AGCCCGGGCC GCCGCCTCTA ACCAAGCTGC TCGGGCCGCT 
GCCACTGCTG CCCGTGCCAC CTACAATCAG GTGGTCACTA ACCACCACCC GGTGGCCACA 
CACCAGGCGT CAGGAGGCGA TACCCAGCCC ATGACATCTG CTGCCCAGGC TCCGGCAGCC 
ACCCCTGAGA CAAGCGTCGC TTCTCCGCAC AGCTCCCGGA TGCTAGTCAA TAGCGAGATG 
GCTGCCCCTG GGGCTCCAGC AAGGTCTCCA CAGCCGCAGA CATCCTCCCA GGCCCAGGAG 
GCTGCGGCCG AGGGCCCTAG TACGGCCTGT GCTTTCCCCC AGGCCTCGCG TGCAAGTGAG 
ATGGATGCCA CCCGGCCCAA GACAGCCTTC CTGGGTCAGA ACGACGCCTT TGATTTCAGC 
CAGCCGGCAG GTGTCAGTGG CATGGCCTTC CCACGCCCCA AGAGACCTGC CCCGGCCCAA 
GAGGCTGCCA CAGAGGGCCC CAGTGTTGCC TCCAGGGGCA CCCAGGCAGC CTCTGCCGGG 
GAGGGGGCGG CCACCCGGCC CAAGACGACC AAGTCCGGGA AGGCTCTCGC CAAGACTCGC 
TGGGTGGAGC CTCAGAATGT TGTGGCAGCA GCTGCCGCCA AGGCCAAGAT GGCCACGAGC 
ATCCCTGAGC CTGAGAGTGC AGCTGCCACT TCTCAGCAGA GTGCGGAGCC CTGGGCCAGA 
ATGGGAGGCA AGAGGACAAA GAAGTCCAAG CACCTGGATG ACGAATATGA GAGCGGCGAG 
GAGGAGAGAG AGCCTCCTGC GGTCCCACCA ACCTGGAGGG CATCGCAGCC CCTGCTGACG 
ACTGCGCGGC CTCAGGTGGC CCCTCGGCCC TCCATGGCCC TGAGGTCCCA GGTACCCTCA 
AGGCATGTGC TGTGCTTGCC ACCCCGCAAT GTGACCCTTC TGCAAGAGAG GGCAAATAAG 
TTGGTGAAAT ATCTGATGAT TAAAGACTAC AAGAAGATCC CCATCAAGCG CTCAGACATG 
CTGAAGGATG TCATCCGAGA ATATGACGAA CACTTCCCTG AGATCATTGA ACGAGCAACG 
TACACTCTGG AAAAGAAGTT CGGGATCCAC CTGAAGGAAA TTGACAAGGA AGAGCACCTG 
TACATTCTCG TCTGCACACG GGATTCGTCA GCCCGCCTCC TGGGAAAGAC CAAGGATACT 
CCCAGGCTGA GTCTCCTCTT GGTGATTCTG GGTGTCATCT TCATGAACGG CAACCGCGCC 
AGCGAGGCTG TCCTCTGGGA GGCACTCCGC AAGATGGGAC TGCGCCCCGG GGTGAGGCAC 
CCATTCCTTG GCGATCTGAG GAAACTCATT ACAGAGGACT TTGTGAAGCA GAAGTACTTG 
GAATACAAGA AGGTCCCCAA TAGCAGCCCA CCCGAGTATG AGTTTCTCTG GGGTCTGCGC 
GCCTGCCATG AGACCAGCAA GATGAGGGTG CTGAGATTCA TCGCCCAGTA TCAGAACCGA 
GACCCCCGGG AATGGAGGGC TCATTTCTTG GAGGCTGTGG ATGATGCTTT CAAGACGATG 
GATGTGGATA TGGCCGAGGA GCATGCCAGG GCCCAGATGA GGGCCCAGAT GAACATCGGG 
GAGGAAGCTC TGATTGGACG GTGGAGCTGG GACGATATCC AGGTGGAGCT CCTGACCTGG 
GATGAGGACG GAGATTTTGG CGACGCCTGG TCCAGGATCC CCTTCGCTTT CTGGGCCAGA 
TACCATCAGT ACATTCTGAA TAGCAACCGT GCCAACAGGA GAGGTACCTG GAGGGCTGGT 
GTCAGCAGTG GCACCAATGG CGCGGCCAGC GCCAGCATGC TCGATGGCCC CAGCACCAGC 
TCCACCATCC GGACCAGAAA CGCCGCCAGA ACTAGTGCCA GCTTCTTCTC CTGGATTCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED4B ( XM_024987701.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843469.1 Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa238222
Clone ID Related Accession (Same CDS sequence)	XM_024987700.1 , XM_024987701.1
Accession Version	XM_024987701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	melanoma-associated antigen D4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAGCTACCG CAAGGAATCT GAAGGGTACA ACGTTGAAGA CATGGACGAG 
GGTAGTGATG AAGTCGGGGA GGAAGACATG GTTGAAGGCA ACGACTATGA AGAATTTGGT 
GCTTTCGGAG GCTACGGCGC CCTCACCAGC TTTGACATCC GCATCCTCAG AGCCTTTGGG 
AGCTTGGGTC CAGGCTTTCG CATCTTAGCG AATGAGCCCT GGGAACTGGA AAACCCAGTG 
CTGGCCAGAA CTCTGCTGGA GGCATTTCGG ATGGATCCAG AAACACTTGC CAATGAGACG 
GCTGCCCGTG CTGCCAACGT AGCCCGGGCC GCCGCCTCTA ACCAAGCTGC TCGGGCCGCT 
GCCACTGCTG CCCGTGCCAC CTACAATCAG GTGGTCACTA ACCACCACCC GGTGGCCACA 
CACCAGGCGT CAGGAGGCGA TACCCAGCCC ATGACATCTG CTGCCCAGGC TCCGGCAGCC 
ACCCCTGAGA CAAGCGTCGC TTCTCCGCAC AGCTCCCGGA TGCTAGTCAA TAGCGAGATG 
GCTGCCCCTG GGGCTCCAGC AAGGTCTCCA CAGCCGCAGA CATCCTCCCA GGCCCAGGAG 
GCTGCGGCCG AGGGCCCTAG TACGGCCTGT GCTTTCCCCC AGGCCTCGCG TGCAAGTGAG 
ATGGATGCCA CCCGGCCCAA GACAGCCTTC CTGGGTCAGA ACGACGCCTT TGATTTCAGC 
CAGCCGGCAG GTGTCAGTGG CATGGCCTTC CCACGCCCCA AGAGACCTGC CCCGGCCCAA 
GAGGCTGCCA CAGAGGGCCC CAGTGTTGCC TCCAGGGGCA CCCAGGCAGC CTCTGCCGGG 
GAGGGGGCGG CCACCCGGCC CAAGACGACC AAGTCCGGGA AGGCTCTCGC CAAGACTCGC 
TGGGTGGAGC CTCAGAATGT TGTGGCAGCA GCTGCCGCCA AGGCCAAGAT GGCCACGAGC 
ATCCCTGAGC CTGAGAGTGC AGCTGCCACT TCTCAGCAGA GTGCGGAGCC CTGGGCCAGA 
ATGGGAGGCA AGAGGACAAA GAAGTCCAAG CACCTGGATG ACGAATATGA GAGCGGCGAG 
GAGGAGAGAG AGCCTCCTGC GGTCCCACCA ACCTGGAGGG CATCGCAGCC CCTGCTGACG 
ACTGCGCGGC CTCAGGTGGC CCCTCGGCCC TCCATGGCCC TGAGGTCCCA GGTACCCTCA 
AGGCATGTGC TGTGCTTGCC ACCCCGCAAT GTGACCCTTC TGCAAGAGAG GGCAAATAAG 
TTGGTGAAAT ATCTGATGAT TAAAGACTAC AAGAAGATCC CCATCAAGCG CTCAGACATG 
CTGAAGGATG TCATCCGAGA ATATGACGAA CACTTCCCTG AGATCATTGA ACGAGCAACG 
TACACTCTGG AAAAGAAGTT CGGGATCCAC CTGAAGGAAA TTGACAAGGA AGAGCACCTG 
TACATTCTCG TCTGCACACG GGATTCGTCA GCCCGCCTCC TGGGAAAGAC CAAGGATACT 
CCCAGGCTGA GTCTCCTCTT GGTGATTCTG GGTGTCATCT TCATGAACGG CAACCGCGCC 
AGCGAGGCTG TCCTCTGGGA GGCACTCCGC AAGATGGGAC TGCGCCCCGG GGTGAGGCAC 
CCATTCCTTG GCGATCTGAG GAAACTCATT ACAGAGGACT TTGTGAAGCA GAAGTACTTG 
GAATACAAGA AGGTCCCCAA TAGCAGCCCA CCCGAGTATG AGTTTCTCTG GGGTCTGCGC 
GCCTGCCATG AGACCAGCAA GATGAGGGTG CTGAGATTCA TCGCCCAGTA TCAGAACCGA 
GACCCCCGGG AATGGAGGGC TCATTTCTTG GAGGCTGTGG ATGATGCTTT CAAGACGATG 
GATGTGGATA TGGCCGAGGA GCATGCCAGG GCCCAGATGA GGGCCCAGAT GAACATCGGG 
GAGGAAGCTC TGATTGGACG GTGGAGCTGG GACGATATCC AGGTGGAGCT CCTGACCTGG 
GATGAGGACG GAGATTTTGG CGACGCCTGG TCCAGGATCC CCTTCGCTTT CTGGGCCAGA 
TACCATCAGT ACATTCTGAA TAGCAACCGT GCCAACAGGA GAGGTACCTG GAGGGCTGGT 
GTCAGCAGTG GCACCAATGG CGCGGCCAGC GCCAGCATGC TCGATGGCCC CAGCACCAGC 
TCCACCATCC GGACCAGAAA CGCCGCCAGA ACTAGTGCCA GCTTCTTCTC CTGGATTCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843469.1
CDS	232..2454
Translation	MAEGSYRKESEGYNVEDMDEGSDEVGEEDMVEGNDYEEFGAFGGYGALTSFDIRILRAFGSLGPGFRILANEPWELENPVLARTLLEAFRMDPETLANETAARAANVARAAASNQAARAAATAARATYNQVVTNHHPVATHQASGGDTQPMTSAAQAPAATPETSVASPHSSRMLVNSEMAAPGAPARSPQPQTSSQAQEAAAEGPSTACAFPQASRASEMDATRPKTAFLGQNDAFDFSQPAGVSGMAFPRPKRPAPAQEAATEGPSVASRGTQAASAGEGAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPESAAATSQQSAEPWARMGGKRTKKSKHLDDEYESGEEEREPPAVPPTWRASQPLLTTARPQVAPRPSMALRSQVPSRHVLCLPPRNVTLLQERANKLVKYLMIKDYKKIPIKRSDMLKDVIREYDEHFPEIIERATYTLEKKFGIHLKEIDKEEHLYILVCTRDSSARLLGKTKDTPRLSLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPFLGDLRKLITEDFVKQKYLEYKKVPNSSPPEYEFLWGLRACHETSKMRVLRFIAQYQNRDPREWRAHFLEAVDDAFKTMDVDMAEEHARAQMRAQMNIGEEALIGRWSWDDIQVELLTWDEDGDFGDAWSRIPFAFWARYHQYILNSNRANRRGTWRAGVSSGTNGAASASMLDGPSTSSTIRTRNAARTSASFFSWIQ

Target ORF information:

RefSeq Version	XM_024987701.1
Organism	Bos taurus(cattle)
Definition	Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987701.1

ORF Insert Sequence:

ATGGCAGAGG GAAGCTACCG CAAGGAATCT GAAGGGTACA ACGTTGAAGA CATGGACGAG 
GGTAGTGATG AAGTCGGGGA GGAAGACATG GTTGAAGGCA ACGACTATGA AGAATTTGGT 
GCTTTCGGAG GCTACGGCGC CCTCACCAGC TTTGACATCC GCATCCTCAG AGCCTTTGGG 
AGCTTGGGTC CAGGCTTTCG CATCTTAGCG AATGAGCCCT GGGAACTGGA AAACCCAGTG 
CTGGCCAGAA CTCTGCTGGA GGCATTTCGG ATGGATCCAG AAACACTTGC CAATGAGACG 
GCTGCCCGTG CTGCCAACGT AGCCCGGGCC GCCGCCTCTA ACCAAGCTGC TCGGGCCGCT 
GCCACTGCTG CCCGTGCCAC CTACAATCAG GTGGTCACTA ACCACCACCC GGTGGCCACA 
CACCAGGCGT CAGGAGGCGA TACCCAGCCC ATGACATCTG CTGCCCAGGC TCCGGCAGCC 
ACCCCTGAGA CAAGCGTCGC TTCTCCGCAC AGCTCCCGGA TGCTAGTCAA TAGCGAGATG 
GCTGCCCCTG GGGCTCCAGC AAGGTCTCCA CAGCCGCAGA CATCCTCCCA GGCCCAGGAG 
GCTGCGGCCG AGGGCCCTAG TACGGCCTGT GCTTTCCCCC AGGCCTCGCG TGCAAGTGAG 
ATGGATGCCA CCCGGCCCAA GACAGCCTTC CTGGGTCAGA ACGACGCCTT TGATTTCAGC 
CAGCCGGCAG GTGTCAGTGG CATGGCCTTC CCACGCCCCA AGAGACCTGC CCCGGCCCAA 
GAGGCTGCCA CAGAGGGCCC CAGTGTTGCC TCCAGGGGCA CCCAGGCAGC CTCTGCCGGG 
GAGGGGGCGG CCACCCGGCC CAAGACGACC AAGTCCGGGA AGGCTCTCGC CAAGACTCGC 
TGGGTGGAGC CTCAGAATGT TGTGGCAGCA GCTGCCGCCA AGGCCAAGAT GGCCACGAGC 
ATCCCTGAGC CTGAGAGTGC AGCTGCCACT TCTCAGCAGA GTGCGGAGCC CTGGGCCAGA 
ATGGGAGGCA AGAGGACAAA GAAGTCCAAG CACCTGGATG ACGAATATGA GAGCGGCGAG 
GAGGAGAGAG AGCCTCCTGC GGTCCCACCA ACCTGGAGGG CATCGCAGCC CCTGCTGACG 
ACTGCGCGGC CTCAGGTGGC CCCTCGGCCC TCCATGGCCC TGAGGTCCCA GGTACCCTCA 
AGGCATGTGC TGTGCTTGCC ACCCCGCAAT GTGACCCTTC TGCAAGAGAG GGCAAATAAG 
TTGGTGAAAT ATCTGATGAT TAAAGACTAC AAGAAGATCC CCATCAAGCG CTCAGACATG 
CTGAAGGATG TCATCCGAGA ATATGACGAA CACTTCCCTG AGATCATTGA ACGAGCAACG 
TACACTCTGG AAAAGAAGTT CGGGATCCAC CTGAAGGAAA TTGACAAGGA AGAGCACCTG 
TACATTCTCG TCTGCACACG GGATTCGTCA GCCCGCCTCC TGGGAAAGAC CAAGGATACT 
CCCAGGCTGA GTCTCCTCTT GGTGATTCTG GGTGTCATCT TCATGAACGG CAACCGCGCC 
AGCGAGGCTG TCCTCTGGGA GGCACTCCGC AAGATGGGAC TGCGCCCCGG GGTGAGGCAC 
CCATTCCTTG GCGATCTGAG GAAACTCATT ACAGAGGACT TTGTGAAGCA GAAGTACTTG 
GAATACAAGA AGGTCCCCAA TAGCAGCCCA CCCGAGTATG AGTTTCTCTG GGGTCTGCGC 
GCCTGCCATG AGACCAGCAA GATGAGGGTG CTGAGATTCA TCGCCCAGTA TCAGAACCGA 
GACCCCCGGG AATGGAGGGC TCATTTCTTG GAGGCTGTGG ATGATGCTTT CAAGACGATG 
GATGTGGATA TGGCCGAGGA GCATGCCAGG GCCCAGATGA GGGCCCAGAT GAACATCGGG 
GAGGAAGCTC TGATTGGACG GTGGAGCTGG GACGATATCC AGGTGGAGCT CCTGACCTGG 
GATGAGGACG GAGATTTTGG CGACGCCTGG TCCAGGATCC CCTTCGCTTT CTGGGCCAGA 
TACCATCAGT ACATTCTGAA TAGCAACCGT GCCAACAGGA GAGGTACCTG GAGGGCTGGT 
GTCAGCAGTG GCACCAATGG CGCGGCCAGC GCCAGCATGC TCGATGGCCC CAGCACCAGC 
TCCACCATCC GGACCAGAAA CGCCGCCAGA ACTAGTGCCA GCTTCTTCTC CTGGATTCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED4B ( XM_015461704.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317190.1 Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa89389
Clone ID Related Accession (Same CDS sequence)	XM_015461704.1
Accession Version	XM_015461704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	melanoma-associated antigen D4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104737.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAGCTACCG CAAGGAATCT GAAGGGTACA ACGTTGAAGA CATGGACGAG 
GGTAGTGATG AAGTCGGGGA GGAAGACATG GTTGAAGGCA ACGACTATGA AGAATTTGGT 
GCTTTCGGAG GCTACGGCGC CCTCACCAGC TTTGACATCC GCATCCTCAG AGCCTTTGGG 
AGCTTGGGTC CAGGCTTTCG CATCTTAGCG ACCGATCCCT GGGAACTGGA AAACCCAGTG 
CTGGCCAGAA CTCTGCTGGA GGCATTTCGG ATGGATCCAG AAACACTTGC CAATGAGACG 
GCTGCCCGTG CTGCCAACGT AGCCCGGGCC GCCGCCTCTA ACCAAGCTGC TCGGGCCGCT 
GCCACTGCTG CCCGTGCCAC CTACAATCAG GTGGTCACTA ACCACCACCC GGTGGCCACA 
CACCAGGCGT CAGGAGGCGA TACCCAGCCC ATGACATCTG CTGCCCAGGC TCCGGCAGCC 
ACCCCTGAGA CAAGCGTCGC TTCTCCGCAC AGCTCCCGGA TGCTAGTCAA TAGCGAGATG 
GCTGCCCCTG GGGCTCCAGC AAGGTCTCCA CAGCCGCAGA CATCCTCCCA GGCCCAGGAG 
GCTGCGGCCG AGGGCCCTAG TACGGCCTGT GCTTTCCCCC AGGCCTCGCG TGCAAGTGAG 
ATGGATGCCA CCCGGCCCAA GACAGCCTTC CTGGGTCAGA ACGACGCCTT TGATTTCAGC 
CAGCCGGCAG GTGTCAGTGG CATGGCCTTC CCACGCCCCA AGAGACCTGC CCCGGCCCAA 
GAGGCTGCCA CAGAGGGCCC CAGTGTTGCC TCCAGGGGCA CCCAGGCAGC CTCTGCCGGG 
GAGGGGGCGG CCACCCGGCC CAAGACGACC AAGTCCGGGA AGGCTCTCGC CAAGACTCGC 
TGGGTGGAGC CTCAGAATGT TGTGGCAGCA GCTGCCGCCA AGGCCAAGAT GGCCACGAGC 
ATCCCTGAGC CTGAGAGTGC AGCTGCCACT TCTCAGCAGA GTGCGGAGCC CTGGGCCAGA 
ATGGGAGGCA AGAGGACAAA GAAGTCCAAG CACCTGGATG ACGAATATGA GAGCGGCGAG 
GAGGAGAGAG AGCCTCCTGC GGTCCCACCA ACCTGGAGGG CATCGCAGCC CCTGCTGACG 
ACTGCGCGGC CTCAGGTGGC CCCTCGGCCC TCCATGGCCC TGAGGTCCCA GGTACCCTCA 
AGGCATGTGC TGTGCTTGCC ACCCCGCAAT GTGACCCTTC TGCAAGAGAG GGCAAATAAG 
TTGGTGAAAT ATCTGATGAT TAAAGACTAC AAGAAGATCC CCATCAAGCG CTCAGACATG 
CTGAAGGATG TCATCCGAGA ATATGACGAA CACTTCCCTG AGATCATTGA ACGAGCAACG 
TACACTCTGG AAAAGAAGTT CGGGATCCAC CTGAAGGAAA TTGACAAGGA AGAGCACCTG 
TACATTCTCG TCTGCACACG GGATTCGTCA GCCCGCCTCC TGGGAAAGAC CAAGGATACT 
CCCAGGCTGA GTCTCCTCTT GGTGATTCTG GGTGTCATCT TCATGAACGG CAACCGCGCC 
AGCGAGGCTG TCCTCTGGGA GGCACTCCGC AAGATGGGAC TGCGCCCCGG GGTGAGGCAC 
CCATTCCTTG GCGATCTGAG GAAACTCATT ACAGAGGACT TTGTGAAGCA GAAGTACTTG 
GAATACAAGA AGGTCCCCAA TAGCAGCCCA CCCGAGTATG AGTTTCTCTG GGGTCTGCGC 
GCCTGCCATG AGACCAGCAA GATGAGGGTG CTGAGATTCA TCGCCCAGTA TCAGAACCGA 
GACCCCCGGG AATGGAGGGC TCATTTCTTG GAGGCTGTGG ATGATGCTTT CAAGACGATG 
GATGTGGATA TGGCCGAGGA GCATGCCAGG GCCCAGATGA GGGCCCAGAT GAACATCGGG 
GAGGAAGCTC TGATTGGACG GTGGAGCTGG GACGATATCC AGGTGGAGCT CCTGACCTGG 
GATGAGGACG GAGATTTTGG CGACGCCTGG TCCAGGATCC CCTTCGCTTT CTGGGCCAGA 
TACCATCAGT ACATTCTGAA TAGCAACCGT GCCAACAGGA GAGGTACCTG GAGGGCTGGT 
GTCAGCAGTG GCACCAATGG CGCGGCCAGC GCCAGCATGC TCGATGGCCC CAGCACCAGC 
TCCACCATCC GGACCAGAAA CGCCGCCAGA ACTAGTGCCA GCTTCTTCTC CTGGATTCAG 
CAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317190.1
CDS	107..2335
Translation	MAEGSYRKESEGYNVEDMDEGSDEVGEEDMVEGNDYEEFGAFGGYGALTSFDIRILRAFGSLGPGFRILATDPWELENPVLARTLLEAFRMDPETLANETAARAANVARAAASNQAARAAATAARATYNQVVTNHHPVATHQASGGDTQPMTSAAQAPAATPETSVASPHSSRMLVNSEMAAPGAPARSPQPQTSSQAQEAAAEGPSTACAFPQASRASEMDATRPKTAFLGQNDAFDFSQPAGVSGMAFPRPKRPAPAQEAATEGPSVASRGTQAASAGEGAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPESAAATSQQSAEPWARMGGKRTKKSKHLDDEYESGEEEREPPAVPPTWRASQPLLTTARPQVAPRPSMALRSQVPSRHVLCLPPRNVTLLQERANKLVKYLMIKDYKKIPIKRSDMLKDVIREYDEHFPEIIERATYTLEKKFGIHLKEIDKEEHLYILVCTRDSSARLLGKTKDTPRLSLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPFLGDLRKLITEDFVKQKYLEYKKVPNSSPPEYEFLWGLRACHETSKMRVLRFIAQYQNRDPREWRAHFLEAVDDAFKTMDVDMAEEHARAQMRAQMNIGEEALIGRWSWDDIQVELLTWDEDGDFGDAWSRIPFAFWARYHQYILNSNRANRRGTWRAGVSSGTNGAASASMLDGPSTSSTIRTRNAARTSASFFSWIQQP

Target ORF information:

RefSeq Version	XM_015461704.1
Organism	Bos taurus(cattle)
Definition	Bos taurus melanoma antigen family D, 4B (MAGED4B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461704.1

ORF Insert Sequence:

ATGGCAGAGG GAAGCTACCG CAAGGAATCT GAAGGGTACA ACGTTGAAGA CATGGACGAG 
GGTAGTGATG AAGTCGGGGA GGAAGACATG GTTGAAGGCA ACGACTATGA AGAATTTGGT 
GCTTTCGGAG GCTACGGCGC CCTCACCAGC TTTGACATCC GCATCCTCAG AGCCTTTGGG 
AGCTTGGGTC CAGGCTTTCG CATCTTAGCG ACCGATCCCT GGGAACTGGA AAACCCAGTG 
CTGGCCAGAA CTCTGCTGGA GGCATTTCGG ATGGATCCAG AAACACTTGC CAATGAGACG 
GCTGCCCGTG CTGCCAACGT AGCCCGGGCC GCCGCCTCTA ACCAAGCTGC TCGGGCCGCT 
GCCACTGCTG CCCGTGCCAC CTACAATCAG GTGGTCACTA ACCACCACCC GGTGGCCACA 
CACCAGGCGT CAGGAGGCGA TACCCAGCCC ATGACATCTG CTGCCCAGGC TCCGGCAGCC 
ACCCCTGAGA CAAGCGTCGC TTCTCCGCAC AGCTCCCGGA TGCTAGTCAA TAGCGAGATG 
GCTGCCCCTG GGGCTCCAGC AAGGTCTCCA CAGCCGCAGA CATCCTCCCA GGCCCAGGAG 
GCTGCGGCCG AGGGCCCTAG TACGGCCTGT GCTTTCCCCC AGGCCTCGCG TGCAAGTGAG 
ATGGATGCCA CCCGGCCCAA GACAGCCTTC CTGGGTCAGA ACGACGCCTT TGATTTCAGC 
CAGCCGGCAG GTGTCAGTGG CATGGCCTTC CCACGCCCCA AGAGACCTGC CCCGGCCCAA 
GAGGCTGCCA CAGAGGGCCC CAGTGTTGCC TCCAGGGGCA CCCAGGCAGC CTCTGCCGGG 
GAGGGGGCGG CCACCCGGCC CAAGACGACC AAGTCCGGGA AGGCTCTCGC CAAGACTCGC 
TGGGTGGAGC CTCAGAATGT TGTGGCAGCA GCTGCCGCCA AGGCCAAGAT GGCCACGAGC 
ATCCCTGAGC CTGAGAGTGC AGCTGCCACT TCTCAGCAGA GTGCGGAGCC CTGGGCCAGA 
ATGGGAGGCA AGAGGACAAA GAAGTCCAAG CACCTGGATG ACGAATATGA GAGCGGCGAG 
GAGGAGAGAG AGCCTCCTGC GGTCCCACCA ACCTGGAGGG CATCGCAGCC CCTGCTGACG 
ACTGCGCGGC CTCAGGTGGC CCCTCGGCCC TCCATGGCCC TGAGGTCCCA GGTACCCTCA 
AGGCATGTGC TGTGCTTGCC ACCCCGCAAT GTGACCCTTC TGCAAGAGAG GGCAAATAAG 
TTGGTGAAAT ATCTGATGAT TAAAGACTAC AAGAAGATCC CCATCAAGCG CTCAGACATG 
CTGAAGGATG TCATCCGAGA ATATGACGAA CACTTCCCTG AGATCATTGA ACGAGCAACG 
TACACTCTGG AAAAGAAGTT CGGGATCCAC CTGAAGGAAA TTGACAAGGA AGAGCACCTG 
TACATTCTCG TCTGCACACG GGATTCGTCA GCCCGCCTCC TGGGAAAGAC CAAGGATACT 
CCCAGGCTGA GTCTCCTCTT GGTGATTCTG GGTGTCATCT TCATGAACGG CAACCGCGCC 
AGCGAGGCTG TCCTCTGGGA GGCACTCCGC AAGATGGGAC TGCGCCCCGG GGTGAGGCAC 
CCATTCCTTG GCGATCTGAG GAAACTCATT ACAGAGGACT TTGTGAAGCA GAAGTACTTG 
GAATACAAGA AGGTCCCCAA TAGCAGCCCA CCCGAGTATG AGTTTCTCTG GGGTCTGCGC 
GCCTGCCATG AGACCAGCAA GATGAGGGTG CTGAGATTCA TCGCCCAGTA TCAGAACCGA 
GACCCCCGGG AATGGAGGGC TCATTTCTTG GAGGCTGTGG ATGATGCTTT CAAGACGATG 
GATGTGGATA TGGCCGAGGA GCATGCCAGG GCCCAGATGA GGGCCCAGAT GAACATCGGG 
GAGGAAGCTC TGATTGGACG GTGGAGCTGG GACGATATCC AGGTGGAGCT CCTGACCTGG 
GATGAGGACG GAGATTTTGG CGACGCCTGG TCCAGGATCC CCTTCGCTTT CTGGGCCAGA 
TACCATCAGT ACATTCTGAA TAGCAACCGT GCCAACAGGA GAGGTACCTG GAGGGCTGGT 
GTCAGCAGTG GCACCAATGG CGCGGCCAGC GCCAGCATGC TCGATGGCCC CAGCACCAGC 
TCCACCATCC GGACCAGAAA CGCCGCCAGA ACTAGTGCCA GCTTCTTCTC CTGGATTCAG 
CAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MAGED4B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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