CAPN5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAPN5
Entrez Gene ID	726
Full Name	calpain 5
Synonyms	ADNIV,HTRA3,VRNI,nCL-3
General protein information	Preferred Names calpain 5 Names calpain-5 calpain htra-3 new calpain 3 testis tissue sperm-binding protein Li 91mP
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.5
Summary	Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq, Jul 2008].
Disorder MIM:	602537
Disorder Html:	Vitreoretinopathy, neovascular inflammatory, 193235 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004055.5	NP_004046.2	calpain-5
XM_017018223.2	XP_016873712.2	calpain-5 isoform X2
XM_011545225.1	XP_011543527.1	calpain-5 isoform X1
NM_004055.4	NP_004046.2	calpain-5
XM_017018223.1	XP_016873712.1	calpain-5 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAPN5	XP_003313296.2
Mus musculus (house mouse)	Capn5	NP_031628.1
Danio rerio (zebrafish)	capn5a	NP_001073476.1
Canis lupus familiaris (dog)	CAPN5	XP_005633518.1
Danio rerio (zebrafish)	capn5b	XP_001345114.2
Caenorhabditis elegans (roundworm)	tra-3	NP_502751.1
Xenopus tropicalis (tropical clawed frog)	capn5	NP_001006736.1
Homo sapiens (human)	CAPN5	NP_004046.2
	LOC698077	XP_002799769.1
Bos taurus (cattle)	CAPN5	NP_001179823.1
Rattus norvegicus (Norway rat)	Capn5	NP_604456.1
Gallus gallus (chicken)	CAPN5	XP_417278.2

Gene Ontology
Bibliography

Related articles in PubMed

Small-angle X-ray scattering of calpain-5 reveals a highly open conformation among calpains.
Gakhar L, Bassuk AG, Velez G, Khan S, Yang J, Tsang SH, Mahajan VB
Journal of structural biology196(3)309-318(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The relative domain rotation of 60-100 degrees we found for mini-calpain-5 (a non-classical calpain) is significantly greater than the largest rotation previously observed for a classical calpain
Title: Small-angle X-ray scattering of calpain-5 reveals a highly open conformation among calpains.

CAPN5 localization at the photoreceptor synapse and with mitochondria explains the neural circuitry phenotype in human CAPN5 disease alleles.
Title: Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses.

CAPN5 mutation in hereditary uveitis: the R243L mutation increases calpain catalytic activity and triggers intraocular inflammation
Title: CAPN5 mutation in hereditary uveitis: the R243L mutation increases calpain catalytic activity and triggers intraocular inflammation in a mouse model.

A novel CAPN5 (c.750G>T, p.Lys250Asn) missense mutation causes uveitis and neovascular retinal detachment.
Title: Structural modeling of a novel CAPN5 mutation that causes uveitis and neovascular retinal detachment.

CAPN5 expression can be suppressed by shRNA-based RNA interference
Title: CAPN5 gene silencing by short hairpin RNA interference.

The following CAPN5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAPN5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18113	NM_004055.5 Latest version!	Homo sapiens calpain 5 (CAPN5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu18113	XM_017018223.2 Latest version!	Homo sapiens calpain 5 (CAPN5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu56531	XM_011545225.1 Latest version!	Homo sapiens calpain 5 (CAPN5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu92471	XM_017018223.1 Latest version!	Homo sapiens calpain 5 (CAPN5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu18113	NM_004055.4	Homo sapiens calpain 5 (CAPN5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAPN5 ( NM_004055.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004046.2 Homo sapiens calpain 5 (CAPN5), mRNA.

Price & Availability↑

CloneID	OHu18113
Clone ID Related Accession (Same CDS sequence)	NM_004055.5 , XM_017018223.2 , NM_004055.4
Accession Version	NM_004055.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	calpain-5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC018123.1 and AP000752.4. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004055.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005714.1, SRR1803617.171109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000648180.1/ ENSP00000498132.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTCTCGT GTGTGAAGCC CTATGAGGAC CAGAACTACT CAGCCCTGAG GCGGGACTGC 
CGGCGCAGGA AGGTGCTCTT CGAGGACCCC CTCTTCCCCG CCACTGACGA CTCACTCTAC 
TATAAGGGCA CGCCGGGGCC CGCCGTCAGG TGGAAGCGAC CCAAGGGCAT CTGCGAGGAC 
CCCCGCCTCT TTGTGGATGG CATCAGCTCC CACGACCTGC ACCAGGGCCA GGTGGGCAAC 
TGCTGGTTTG TGGCAGCCTG CTCGTCACTT GCCTCCCGGG AGTCGCTGTG GCAAAAGGTC 
ATCCCAGACT GGAAGGAGCA GGAATGGGAC CCCGAAAAGC CCAACGCCTA CGCGGGCATC 
TTCCACTTCC ACTTCTGGCG CTTCGGGGAA TGGGTGGACG TGGTCATCGA TGACCGGCTG 
CCCACAGTCA ACAACCAGCT CATCTACTGC CACTCCAACT CCCGCAATGA GTTTTGGTGC 
GCCCTAGTGG AGAAGGCCTA TGCCAAACTG GCAGGCTGTT ACCAGGCCCT GGATGGAGGC 
AACACAGCAG ACGCACTGGT GGACTTCACG GGTGGTGTTT CTGAGCCCAT CGACCTGACC 
GAGGGTGACT TTGCCAACGA TGAGACTAAG AGGAACCAGC TCTTTGAGCG CATGTTAAAG 
GTGCACAGCC GGGGCGGCCT CATCAGTGCC TCCATCAAGG CAGTGACAGC AGCTGACATG 
GAGGCCCGCC TGGCGTGCGG CCTGGTAAAG GGCCACGCAT ACGCCGTCAC TGATGTGCGC 
AAGGTGCGCC TGGGCCACGG CCTACTGGCC TTCTTCAAGT CAGAGAAGTT GGACATGATC 
CGCCTGCGCA ACCCCTGGGG CGAGCGGGAG TGGAACGGGC CCTGGAGTGA CACCTCGGAG 
GAGTGGCAGA AAGTGAGCAA GAGTGAGCGG GAGAAGATGG GTGTGACCGT GCAGGACGAC 
GGTGAGTTCT GGATGACCTT CGAGGACGTG TGCCGGTACT TCACGGACAT CATCAAGTGC 
CGCGTGATCA ACACATCCCA CCTGAGCATC CACAAGACGT GGGAGGAGGC CCGGCTGCAT 
GGCGCCTGGA CGCTGCATGA GGACCCGCGA CAGAACCGCG GTGGCGGCTG CATCAACCAC 
AAGGACACCT TCTTCCAGAA CCCACAGTAC ATCTTCGAAG TCAAGAAGCC AGAAGATGAA 
GTCCTGATCT GCATCCAGCA GCGGCCAAAG CGGTCTACGC GCCGGGAGGG CAAGGGTGAG 
AACCTGGCCA TTGGCTTTGA CATCTACAAG GTGGAGGAGA ACCGCCAGTA CCGCATGCAC 
AGCCTGCAGC ACAAGGCCGC CAGCTCCATC TACATCAACT CACGCAGCGT CTTCCTGCGC 
ACCGACCAGC CCGAGGGCCG CTATGTCATC ATCCCCACAA CCTTCGAGCC AGGCCACACT 
GGCGAGTTCC TGCTCCGAGT CTTCACTGAT GTGCCCTCCA ACTGCCGGGA GCTGCGCCTG 
GATGAGCCCC CACACACCTG CTGGAGCTCC CTCTGTGGCT ACCCCCAGCT GGTGACCCAG 
GTACATGTCC TGGGAGCTGC TGGCCTCAAG GACTCCCCAA CAGGGGCTAA CTCTTATGTG 
ATCATCAAGT GTGAGGGAGA CAAAGTCCGC TCGGCTGTGC AGAAGGGCAC CTCCACACCA 
GAGTACAATG TGAAAGGCAT CTTCTACCGC AAGAAGCTGA GCCAGCCCAT CACTGTACAG 
GTCTGGAACC ACCGAGTGCT GAAGGATGAA TTTCTGGGCC AGGTGCACCT AAAGGCTGAC 
CCGGACAACC TCCAGGCCCT GCATACCCTC CACCTCCGGG ACCGAAATAG CCGGCAGCCC 
AGCAACCTGC CAGGCACTGT GGCCGTGCAC ATTCTCAGCA GCACCTCCCT CATGGCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004046.2
CDS	160..2082
Translation	MFSCVKPYEDQNYSALRRDCRRRKVLFEDPLFPATDDSLYYKGTPGPAVRWKRPKGICEDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKPNAYAGIFHFHFWRFGEWVDVVIDDRLPTVNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLGHGLLAFFKSEKLDMIRLRNPWGEREWNGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVINTSHLSIHKTWEEARLHGAWTLHEDPRQNRGGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYINSRSVFLRTDQPEGRYVIIPTTFEPGHTGEFLLRVFTDVPSNCRELRLDEPPHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTGANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKADPDNLQALHTLHLRDRNSRQPSNLPGTVAVHILSSTSLMAV

Target ORF information:

RefSeq Version	NM_004055.5
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 5 (CAPN5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004055.5

ORF Insert Sequence:

ATGTTCTCGT GTGTGAAGCC CTATGAGGAC CAGAACTACT CAGCCCTGAG GCGGGACTGC 
CGGCGCAGGA AGGTGCTCTT CGAGGACCCC CTCTTCCCCG CCACTGACGA CTCACTCTAC 
TATAAGGGCA CGCCGGGGCC CGCCGTCAGG TGGAAGCGAC CCAAGGGCAT CTGCGAGGAC 
CCCCGCCTCT TTGTGGATGG CATCAGCTCC CACGACCTGC ACCAGGGCCA GGTGGGCAAC 
TGCTGGTTTG TGGCAGCCTG CTCGTCACTT GCCTCCCGGG AGTCGCTGTG GCAAAAGGTC 
ATCCCAGACT GGAAGGAGCA GGAATGGGAC CCCGAAAAGC CCAACGCCTA CGCGGGCATC 
TTCCACTTCC ACTTCTGGCG CTTCGGGGAA TGGGTGGACG TGGTCATCGA TGACCGGCTG 
CCCACAGTCA ACAACCAGCT CATCTACTGC CACTCCAACT CCCGCAATGA GTTTTGGTGC 
GCCCTAGTGG AGAAGGCCTA TGCCAAACTG GCAGGCTGTT ACCAGGCCCT GGATGGAGGC 
AACACAGCAG ACGCACTGGT GGACTTCACG GGTGGTGTTT CTGAGCCCAT CGACCTGACC 
GAGGGTGACT TTGCCAACGA TGAGACTAAG AGGAACCAGC TCTTTGAGCG CATGTTAAAG 
GTGCACAGCC GGGGCGGCCT CATCAGTGCC TCCATCAAGG CAGTGACAGC AGCTGACATG 
GAGGCCCGCC TGGCGTGCGG CCTGGTAAAG GGCCACGCAT ACGCCGTCAC TGATGTGCGC 
AAGGTGCGCC TGGGCCACGG CCTACTGGCC TTCTTCAAGT CAGAGAAGTT GGACATGATC 
CGCCTGCGCA ACCCCTGGGG CGAGCGGGAG TGGAACGGGC CCTGGAGTGA CACCTCGGAG 
GAGTGGCAGA AAGTGAGCAA GAGTGAGCGG GAGAAGATGG GTGTGACCGT GCAGGACGAC 
GGTGAGTTCT GGATGACCTT CGAGGACGTG TGCCGGTACT TCACGGACAT CATCAAGTGC 
CGCGTGATCA ACACATCCCA CCTGAGCATC CACAAGACGT GGGAGGAGGC CCGGCTGCAT 
GGCGCCTGGA CGCTGCATGA GGACCCGCGA CAGAACCGCG GTGGCGGCTG CATCAACCAC 
AAGGACACCT TCTTCCAGAA CCCACAGTAC ATCTTCGAAG TCAAGAAGCC AGAAGATGAA 
GTCCTGATCT GCATCCAGCA GCGGCCAAAG CGGTCTACGC GCCGGGAGGG CAAGGGTGAG 
AACCTGGCCA TTGGCTTTGA CATCTACAAG GTGGAGGAGA ACCGCCAGTA CCGCATGCAC 
AGCCTGCAGC ACAAGGCCGC CAGCTCCATC TACATCAACT CACGCAGCGT CTTCCTGCGC 
ACCGACCAGC CCGAGGGCCG CTATGTCATC ATCCCCACAA CCTTCGAGCC AGGCCACACT 
GGCGAGTTCC TGCTCCGAGT CTTCACTGAT GTGCCCTCCA ACTGCCGGGA GCTGCGCCTG 
GATGAGCCCC CACACACCTG CTGGAGCTCC CTCTGTGGCT ACCCCCAGCT GGTGACCCAG 
GTACATGTCC TGGGAGCTGC TGGCCTCAAG GACTCCCCAA CAGGGGCTAA CTCTTATGTG 
ATCATCAAGT GTGAGGGAGA CAAAGTCCGC TCGGCTGTGC AGAAGGGCAC CTCCACACCA 
GAGTACAATG TGAAAGGCAT CTTCTACCGC AAGAAGCTGA GCCAGCCCAT CACTGTACAG 
GTCTGGAACC ACCGAGTGCT GAAGGATGAA TTTCTGGGCC AGGTGCACCT AAAGGCTGAC 
CCGGACAACC TCCAGGCCCT GCATACCCTC CACCTCCGGG ACCGAAATAG CCGGCAGCCC 
AGCAACCTGC CAGGCACTGT GGCCGTGCAC ATTCTCAGCA GCACCTCCCT CATGGCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN5 ( NM_004055.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004046.2 Homo sapiens calpain 5 (CAPN5), mRNA.

Price & Availability↑

CloneID	OHu18113
Clone ID Related Accession (Same CDS sequence)	NM_004055.5 , XM_017018223.2 , NM_004055.4
Accession Version	NM_004055.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	calpain-5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC018123.1 and AP000752.4. This sequence is a reference standard in the RefSeqGene project. On Feb 8, 2006 this sequence version replaced NM_004055.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005714.1, SRR1803617.171109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTCGT GTGTGAAGCC CTATGAGGAC CAGAACTACT CAGCCCTGAG GCGGGACTGC 
CGGCGCAGGA AGGTGCTCTT CGAGGACCCC CTCTTCCCCG CCACTGACGA CTCACTCTAC 
TATAAGGGCA CGCCGGGGCC CGCCGTCAGG TGGAAGCGAC CCAAGGGCAT CTGCGAGGAC 
CCCCGCCTCT TTGTGGATGG CATCAGCTCC CACGACCTGC ACCAGGGCCA GGTGGGCAAC 
TGCTGGTTTG TGGCAGCCTG CTCGTCACTT GCCTCCCGGG AGTCGCTGTG GCAAAAGGTC 
ATCCCAGACT GGAAGGAGCA GGAATGGGAC CCCGAAAAGC CCAACGCCTA CGCGGGCATC 
TTCCACTTCC ACTTCTGGCG CTTCGGGGAA TGGGTGGACG TGGTCATCGA TGACCGGCTG 
CCCACAGTCA ACAACCAGCT CATCTACTGC CACTCCAACT CCCGCAATGA GTTTTGGTGC 
GCCCTAGTGG AGAAGGCCTA TGCCAAACTG GCAGGCTGTT ACCAGGCCCT GGATGGAGGC 
AACACAGCAG ACGCACTGGT GGACTTCACG GGTGGTGTTT CTGAGCCCAT CGACCTGACC 
GAGGGTGACT TTGCCAACGA TGAGACTAAG AGGAACCAGC TCTTTGAGCG CATGTTAAAG 
GTGCACAGCC GGGGCGGCCT CATCAGTGCC TCCATCAAGG CAGTGACAGC AGCTGACATG 
GAGGCCCGCC TGGCGTGCGG CCTGGTAAAG GGCCACGCAT ACGCCGTCAC TGATGTGCGC 
AAGGTGCGCC TGGGCCACGG CCTACTGGCC TTCTTCAAGT CAGAGAAGTT GGACATGATC 
CGCCTGCGCA ACCCCTGGGG CGAGCGGGAG TGGAACGGGC CCTGGAGTGA CACCTCGGAG 
GAGTGGCAGA AAGTGAGCAA GAGTGAGCGG GAGAAGATGG GTGTGACCGT GCAGGACGAC 
GGTGAGTTCT GGATGACCTT CGAGGACGTG TGCCGGTACT TCACGGACAT CATCAAGTGC 
CGCGTGATCA ACACATCCCA CCTGAGCATC CACAAGACGT GGGAGGAGGC CCGGCTGCAT 
GGCGCCTGGA CGCTGCATGA GGACCCGCGA CAGAACCGCG GTGGCGGCTG CATCAACCAC 
AAGGACACCT TCTTCCAGAA CCCACAGTAC ATCTTCGAAG TCAAGAAGCC AGAAGATGAA 
GTCCTGATCT GCATCCAGCA GCGGCCAAAG CGGTCTACGC GCCGGGAGGG CAAGGGTGAG 
AACCTGGCCA TTGGCTTTGA CATCTACAAG GTGGAGGAGA ACCGCCAGTA CCGCATGCAC 
AGCCTGCAGC ACAAGGCCGC CAGCTCCATC TACATCAACT CACGCAGCGT CTTCCTGCGC 
ACCGACCAGC CCGAGGGCCG CTATGTCATC ATCCCCACAA CCTTCGAGCC AGGCCACACT 
GGCGAGTTCC TGCTCCGAGT CTTCACTGAT GTGCCCTCCA ACTGCCGGGA GCTGCGCCTG 
GATGAGCCCC CACACACCTG CTGGAGCTCC CTCTGTGGCT ACCCCCAGCT GGTGACCCAG 
GTACATGTCC TGGGAGCTGC TGGCCTCAAG GACTCCCCAA CAGGGGCTAA CTCTTATGTG 
ATCATCAAGT GTGAGGGAGA CAAAGTCCGC TCGGCTGTGC AGAAGGGCAC CTCCACACCA 
GAGTACAATG TGAAAGGCAT CTTCTACCGC AAGAAGCTGA GCCAGCCCAT CACTGTACAG 
GTCTGGAACC ACCGAGTGCT GAAGGATGAA TTTCTGGGCC AGGTGCACCT AAAGGCTGAC 
CCGGACAACC TCCAGGCCCT GCATACCCTC CACCTCCGGG ACCGAAATAG CCGGCAGCCC 
AGCAACCTGC CAGGCACTGT GGCCGTGCAC ATTCTCAGCA GCACCTCCCT CATGGCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004046.2
CDS	186..2108
Translation	MFSCVKPYEDQNYSALRRDCRRRKVLFEDPLFPATDDSLYYKGTPGPAVRWKRPKGICEDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKPNAYAGIFHFHFWRFGEWVDVVIDDRLPTVNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLGHGLLAFFKSEKLDMIRLRNPWGEREWNGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVINTSHLSIHKTWEEARLHGAWTLHEDPRQNRGGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYINSRSVFLRTDQPEGRYVIIPTTFEPGHTGEFLLRVFTDVPSNCRELRLDEPPHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTGANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKADPDNLQALHTLHLRDRNSRQPSNLPGTVAVHILSSTSLMAV

Target ORF information:

RefSeq Version	NM_004055.4
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 5 (CAPN5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004055.4

ORF Insert Sequence:

ATGTTCTCGT GTGTGAAGCC CTATGAGGAC CAGAACTACT CAGCCCTGAG GCGGGACTGC 
CGGCGCAGGA AGGTGCTCTT CGAGGACCCC CTCTTCCCCG CCACTGACGA CTCACTCTAC 
TATAAGGGCA CGCCGGGGCC CGCCGTCAGG TGGAAGCGAC CCAAGGGCAT CTGCGAGGAC 
CCCCGCCTCT TTGTGGATGG CATCAGCTCC CACGACCTGC ACCAGGGCCA GGTGGGCAAC 
TGCTGGTTTG TGGCAGCCTG CTCGTCACTT GCCTCCCGGG AGTCGCTGTG GCAAAAGGTC 
ATCCCAGACT GGAAGGAGCA GGAATGGGAC CCCGAAAAGC CCAACGCCTA CGCGGGCATC 
TTCCACTTCC ACTTCTGGCG CTTCGGGGAA TGGGTGGACG TGGTCATCGA TGACCGGCTG 
CCCACAGTCA ACAACCAGCT CATCTACTGC CACTCCAACT CCCGCAATGA GTTTTGGTGC 
GCCCTAGTGG AGAAGGCCTA TGCCAAACTG GCAGGCTGTT ACCAGGCCCT GGATGGAGGC 
AACACAGCAG ACGCACTGGT GGACTTCACG GGTGGTGTTT CTGAGCCCAT CGACCTGACC 
GAGGGTGACT TTGCCAACGA TGAGACTAAG AGGAACCAGC TCTTTGAGCG CATGTTAAAG 
GTGCACAGCC GGGGCGGCCT CATCAGTGCC TCCATCAAGG CAGTGACAGC AGCTGACATG 
GAGGCCCGCC TGGCGTGCGG CCTGGTAAAG GGCCACGCAT ACGCCGTCAC TGATGTGCGC 
AAGGTGCGCC TGGGCCACGG CCTACTGGCC TTCTTCAAGT CAGAGAAGTT GGACATGATC 
CGCCTGCGCA ACCCCTGGGG CGAGCGGGAG TGGAACGGGC CCTGGAGTGA CACCTCGGAG 
GAGTGGCAGA AAGTGAGCAA GAGTGAGCGG GAGAAGATGG GTGTGACCGT GCAGGACGAC 
GGTGAGTTCT GGATGACCTT CGAGGACGTG TGCCGGTACT TCACGGACAT CATCAAGTGC 
CGCGTGATCA ACACATCCCA CCTGAGCATC CACAAGACGT GGGAGGAGGC CCGGCTGCAT 
GGCGCCTGGA CGCTGCATGA GGACCCGCGA CAGAACCGCG GTGGCGGCTG CATCAACCAC 
AAGGACACCT TCTTCCAGAA CCCACAGTAC ATCTTCGAAG TCAAGAAGCC AGAAGATGAA 
GTCCTGATCT GCATCCAGCA GCGGCCAAAG CGGTCTACGC GCCGGGAGGG CAAGGGTGAG 
AACCTGGCCA TTGGCTTTGA CATCTACAAG GTGGAGGAGA ACCGCCAGTA CCGCATGCAC 
AGCCTGCAGC ACAAGGCCGC CAGCTCCATC TACATCAACT CACGCAGCGT CTTCCTGCGC 
ACCGACCAGC CCGAGGGCCG CTATGTCATC ATCCCCACAA CCTTCGAGCC AGGCCACACT 
GGCGAGTTCC TGCTCCGAGT CTTCACTGAT GTGCCCTCCA ACTGCCGGGA GCTGCGCCTG 
GATGAGCCCC CACACACCTG CTGGAGCTCC CTCTGTGGCT ACCCCCAGCT GGTGACCCAG 
GTACATGTCC TGGGAGCTGC TGGCCTCAAG GACTCCCCAA CAGGGGCTAA CTCTTATGTG 
ATCATCAAGT GTGAGGGAGA CAAAGTCCGC TCGGCTGTGC AGAAGGGCAC CTCCACACCA 
GAGTACAATG TGAAAGGCAT CTTCTACCGC AAGAAGCTGA GCCAGCCCAT CACTGTACAG 
GTCTGGAACC ACCGAGTGCT GAAGGATGAA TTTCTGGGCC AGGTGCACCT AAAGGCTGAC 
CCGGACAACC TCCAGGCCCT GCATACCCTC CACCTCCGGG ACCGAAATAG CCGGCAGCCC 
AGCAACCTGC CAGGCACTGT GGCCGTGCAC ATTCTCAGCA GCACCTCCCT CATGGCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN5 ( XM_017018223.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873712.2 Homo sapiens calpain 5 (CAPN5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu18113
Clone ID Related Accession (Same CDS sequence)	NM_004055.5 , XM_017018223.2 , NM_004055.4
Accession Version	XM_017018223.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	calpain-5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018223.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCGT GTGTGAAGCC CTATGAGGAC CAGAACTACT CAGCCCTGAG GCGGGACTGC 
CGGCGCAGGA AGGTGCTCTT CGAGGACCCC CTCTTCCCCG CCACTGACGA CTCACTCTAC 
TATAAGGGCA CGCCGGGGCC CGCCGTCAGG TGGAAGCGAC CCAAGGGCAT CTGCGAGGAC 
CCCCGCCTCT TTGTGGATGG CATCAGCTCC CACGACCTGC ACCAGGGCCA GGTGGGCAAC 
TGCTGGTTTG TGGCAGCCTG CTCGTCACTT GCCTCCCGGG AGTCGCTGTG GCAAAAGGTC 
ATCCCAGACT GGAAGGAGCA GGAATGGGAC CCCGAAAAGC CCAACGCCTA CGCGGGCATC 
TTCCACTTCC ACTTCTGGCG CTTCGGGGAA TGGGTGGACG TGGTCATCGA TGACCGGCTG 
CCCACAGTCA ACAACCAGCT CATCTACTGC CACTCCAACT CCCGCAATGA GTTTTGGTGC 
GCCCTAGTGG AGAAGGCCTA TGCCAAACTG GCAGGCTGTT ACCAGGCCCT GGATGGAGGC 
AACACAGCAG ACGCACTGGT GGACTTCACG GGTGGTGTTT CTGAGCCCAT CGACCTGACC 
GAGGGTGACT TTGCCAACGA TGAGACTAAG AGGAACCAGC TCTTTGAGCG CATGTTAAAG 
GTGCACAGCC GGGGCGGCCT CATCAGTGCC TCCATCAAGG CAGTGACAGC AGCTGACATG 
GAGGCCCGCC TGGCGTGCGG CCTGGTAAAG GGCCACGCAT ACGCCGTCAC TGATGTGCGC 
AAGGTGCGCC TGGGCCACGG CCTACTGGCC TTCTTCAAGT CAGAGAAGTT GGACATGATC 
CGCCTGCGCA ACCCCTGGGG CGAGCGGGAG TGGAACGGGC CCTGGAGTGA CACCTCGGAG 
GAGTGGCAGA AAGTGAGCAA GAGTGAGCGG GAGAAGATGG GTGTGACCGT GCAGGACGAC 
GGTGAGTTCT GGATGACCTT CGAGGACGTG TGCCGGTACT TCACGGACAT CATCAAGTGC 
CGCGTGATCA ACACATCCCA CCTGAGCATC CACAAGACGT GGGAGGAGGC CCGGCTGCAT 
GGCGCCTGGA CGCTGCATGA GGACCCGCGA CAGAACCGCG GTGGCGGCTG CATCAACCAC 
AAGGACACCT TCTTCCAGAA CCCACAGTAC ATCTTCGAAG TCAAGAAGCC AGAAGATGAA 
GTCCTGATCT GCATCCAGCA GCGGCCAAAG CGGTCTACGC GCCGGGAGGG CAAGGGTGAG 
AACCTGGCCA TTGGCTTTGA CATCTACAAG GTGGAGGAGA ACCGCCAGTA CCGCATGCAC 
AGCCTGCAGC ACAAGGCCGC CAGCTCCATC TACATCAACT CACGCAGCGT CTTCCTGCGC 
ACCGACCAGC CCGAGGGCCG CTATGTCATC ATCCCCACAA CCTTCGAGCC AGGCCACACT 
GGCGAGTTCC TGCTCCGAGT CTTCACTGAT GTGCCCTCCA ACTGCCGGGA GCTGCGCCTG 
GATGAGCCCC CACACACCTG CTGGAGCTCC CTCTGTGGCT ACCCCCAGCT GGTGACCCAG 
GTACATGTCC TGGGAGCTGC TGGCCTCAAG GACTCCCCAA CAGGGGCTAA CTCTTATGTG 
ATCATCAAGT GTGAGGGAGA CAAAGTCCGC TCGGCTGTGC AGAAGGGCAC CTCCACACCA 
GAGTACAATG TGAAAGGCAT CTTCTACCGC AAGAAGCTGA GCCAGCCCAT CACTGTACAG 
GTCTGGAACC ACCGAGTGCT GAAGGATGAA TTTCTGGGCC AGGTGCACCT AAAGGCTGAC 
CCGGACAACC TCCAGGCCCT GCATACCCTC CACCTCCGGG ACCGAAATAG CCGGCAGCCC 
AGCAACCTGC CAGGCACTGT GGCCGTGCAC ATTCTCAGCA GCACCTCCCT CATGGCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873712.2
CDS	104..2026
Translation	MFSCVKPYEDQNYSALRRDCRRRKVLFEDPLFPATDDSLYYKGTPGPAVRWKRPKGICEDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKPNAYAGIFHFHFWRFGEWVDVVIDDRLPTVNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLGHGLLAFFKSEKLDMIRLRNPWGEREWNGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVINTSHLSIHKTWEEARLHGAWTLHEDPRQNRGGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYINSRSVFLRTDQPEGRYVIIPTTFEPGHTGEFLLRVFTDVPSNCRELRLDEPPHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTGANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKADPDNLQALHTLHLRDRNSRQPSNLPGTVAVHILSSTSLMAV

Target ORF information:

RefSeq Version	XM_017018223.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 5 (CAPN5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018223.2

ORF Insert Sequence:

ATGTTCTCGT GTGTGAAGCC CTATGAGGAC CAGAACTACT CAGCCCTGAG GCGGGACTGC 
CGGCGCAGGA AGGTGCTCTT CGAGGACCCC CTCTTCCCCG CCACTGACGA CTCACTCTAC 
TATAAGGGCA CGCCGGGGCC CGCCGTCAGG TGGAAGCGAC CCAAGGGCAT CTGCGAGGAC 
CCCCGCCTCT TTGTGGATGG CATCAGCTCC CACGACCTGC ACCAGGGCCA GGTGGGCAAC 
TGCTGGTTTG TGGCAGCCTG CTCGTCACTT GCCTCCCGGG AGTCGCTGTG GCAAAAGGTC 
ATCCCAGACT GGAAGGAGCA GGAATGGGAC CCCGAAAAGC CCAACGCCTA CGCGGGCATC 
TTCCACTTCC ACTTCTGGCG CTTCGGGGAA TGGGTGGACG TGGTCATCGA TGACCGGCTG 
CCCACAGTCA ACAACCAGCT CATCTACTGC CACTCCAACT CCCGCAATGA GTTTTGGTGC 
GCCCTAGTGG AGAAGGCCTA TGCCAAACTG GCAGGCTGTT ACCAGGCCCT GGATGGAGGC 
AACACAGCAG ACGCACTGGT GGACTTCACG GGTGGTGTTT CTGAGCCCAT CGACCTGACC 
GAGGGTGACT TTGCCAACGA TGAGACTAAG AGGAACCAGC TCTTTGAGCG CATGTTAAAG 
GTGCACAGCC GGGGCGGCCT CATCAGTGCC TCCATCAAGG CAGTGACAGC AGCTGACATG 
GAGGCCCGCC TGGCGTGCGG CCTGGTAAAG GGCCACGCAT ACGCCGTCAC TGATGTGCGC 
AAGGTGCGCC TGGGCCACGG CCTACTGGCC TTCTTCAAGT CAGAGAAGTT GGACATGATC 
CGCCTGCGCA ACCCCTGGGG CGAGCGGGAG TGGAACGGGC CCTGGAGTGA CACCTCGGAG 
GAGTGGCAGA AAGTGAGCAA GAGTGAGCGG GAGAAGATGG GTGTGACCGT GCAGGACGAC 
GGTGAGTTCT GGATGACCTT CGAGGACGTG TGCCGGTACT TCACGGACAT CATCAAGTGC 
CGCGTGATCA ACACATCCCA CCTGAGCATC CACAAGACGT GGGAGGAGGC CCGGCTGCAT 
GGCGCCTGGA CGCTGCATGA GGACCCGCGA CAGAACCGCG GTGGCGGCTG CATCAACCAC 
AAGGACACCT TCTTCCAGAA CCCACAGTAC ATCTTCGAAG TCAAGAAGCC AGAAGATGAA 
GTCCTGATCT GCATCCAGCA GCGGCCAAAG CGGTCTACGC GCCGGGAGGG CAAGGGTGAG 
AACCTGGCCA TTGGCTTTGA CATCTACAAG GTGGAGGAGA ACCGCCAGTA CCGCATGCAC 
AGCCTGCAGC ACAAGGCCGC CAGCTCCATC TACATCAACT CACGCAGCGT CTTCCTGCGC 
ACCGACCAGC CCGAGGGCCG CTATGTCATC ATCCCCACAA CCTTCGAGCC AGGCCACACT 
GGCGAGTTCC TGCTCCGAGT CTTCACTGAT GTGCCCTCCA ACTGCCGGGA GCTGCGCCTG 
GATGAGCCCC CACACACCTG CTGGAGCTCC CTCTGTGGCT ACCCCCAGCT GGTGACCCAG 
GTACATGTCC TGGGAGCTGC TGGCCTCAAG GACTCCCCAA CAGGGGCTAA CTCTTATGTG 
ATCATCAAGT GTGAGGGAGA CAAAGTCCGC TCGGCTGTGC AGAAGGGCAC CTCCACACCA 
GAGTACAATG TGAAAGGCAT CTTCTACCGC AAGAAGCTGA GCCAGCCCAT CACTGTACAG 
GTCTGGAACC ACCGAGTGCT GAAGGATGAA TTTCTGGGCC AGGTGCACCT AAAGGCTGAC 
CCGGACAACC TCCAGGCCCT GCATACCCTC CACCTCCGGG ACCGAAATAG CCGGCAGCCC 
AGCAACCTGC CAGGCACTGT GGCCGTGCAC ATTCTCAGCA GCACCTCCCT CATGGCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN5 ( XM_011545225.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543527.1 Homo sapiens calpain 5 (CAPN5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu56531
Clone ID Related Accession (Same CDS sequence)	XM_011545225.1
Accession Version	XM_011545225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	calpain-5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTGCA CAGGGACGCC CAGCTGTATC TACCTGCTGT CAGCACTTTC TCTGCAGGAC 
CTGGTGCTGG ACTGGGAGCC CCGAGGTGTT CCCCCTCCCC TCCCTGGGGC AGCAGCCACC 
ATGTTCTCGT GTGTGAAGCC CTATGAGGAC CAGAACTACT CAGCCCTGAG GCGGGACTGC 
CGGCGCAGGA AGGTGCTCTT CGAGGACCCC CTCTTCCCCG CCACTGACGA CTCACTCTAC 
TATAAGGGCA CGCCGGGGCC CGCCGTCAGG TGGAAGCGAC CCAAGGGCAT CTGCGAGGAC 
CCCCGCCTCT TTGTGGATGG CATCAGCTCC CACGACCTGC ACCAGGGCCA GGTGGGCAAC 
TGCTGGTTTG TGGCAGCCTG CTCGTCACTT GCCTCCCGGG AGTCGCTGTG GCAAAAGGTC 
ATCCCAGACT GGAAGGAGCA GGAATGGGAC CCCGAAAAGC CCAACGCCTA CGCGGGCATC 
TTCCACTTCC ACTTCTGGCG CTTCGGGGAA TGGGTGGACG TGGTCATCGA TGACCGGCTG 
CCCACAGTCA ACAACCAGCT CATCTACTGC CACTCCAACT CCCGCAATGA GTTTTGGTGC 
GCCCTAGTGG AGAAGGCCTA TGCCAAACTG GCAGGCTGTT ACCAGGCCCT GGATGGAGGC 
AACACAGCAG ACGCACTGGT GGACTTCACG GGTGGTGTTT CTGAGCCCAT CGACCTGACC 
GAGGGTGACT TTGCCAACGA TGAGACTAAG AGGAACCAGC TCTTTGAGCG CATGTTAAAG 
GTGCACAGCC GGGGCGGCCT CATCAGTGCC TCCATCAAGG CAGTGACAGC AGCTGACATG 
GAGGCCCGCC TGGCGTGCGG CCTGGTAAAG GGCCACGCAT ACGCCGTCAC TGATGTGCGC 
AAGGTGCGCC TGGGCCACGG CCTACTGGCC TTCTTCAAGT CAGAGAAGTT GGACATGATC 
CGCCTGCGCA ACCCCTGGGG CGAGCGGGAG TGGAACGGGC CCTGGAGTGA CACCTCGGAG 
GAGTGGCAGA AAGTGAGCAA GAGTGAGCGG GAGAAGATGG GTGTGACCGT GCAGGACGAC 
GGTGAGTTCT GGATGACCTT CGAGGACGTG TGCCGGTACT TCACGGACAT CATCAAGTGC 
CGCGTGATCA ACACATCCCA CCTGAGCATC CACAAGACGT GGGAGGAGGC CCGGCTGCAT 
GGCGCCTGGA CGCTGCATGA GGACCCGCGA CAGAACCGCG GTGGCGGCTG CATCAACCAC 
AAGGACACCT TCTTCCAGAA CCCACAGTAC ATCTTCGAAG TCAAGAAGCC AGAAGATGAA 
GTCCTGATCT GCATCCAGCA GCGGCCAAAG CGGTCTACGC GCCGGGAGGG CAAGGGTGAG 
AACCTGGCCA TTGGCTTTGA CATCTACAAG GTGGAGGAGA ACCGCCAGTA CCGCATGCAC 
AGCCTGCAGC ACAAGGCCGC CAGCTCCATC TACATCAACT CACGCAGCGT CTTCCTGCGC 
ACCGACCAGC CCGAGGGCCG CTATGTCATC ATCCCCACAA CCTTCGAGCC AGGCCACACT 
GGCGAGTTCC TGCTCCGAGT CTTCACTGAT GTGCCCTCCA ACTGCCGGGA GCTGCGCCTG 
GATGAGCCCC CACACACCTG CTGGAGCTCC CTCTGTGGCT ACCCCCAGCT GGTGACCCAG 
GTACATGTCC TGGGAGCTGC TGGCCTCAAG GACTCCCCAA CAGGGGCTAA CTCTTATGTG 
ATCATCAAGT GTGAGGGAGA CAAAGTCCGC TCGGCTGTGC AGAAGGGCAC CTCCACACCA 
GAGTACAATG TGAAAGGCAT CTTCTACCGC AAGAAGCTGA GCCAGCCCAT CACTGTACAG 
GTCTGGAACC ACCGAGTGCT GAAGGATGAA TTTCTGGGCC AGGTGCACCT AAAGGCTGAC 
CCGGACAACC TCCAGGCCCT GCATACCCTC CACCTCCGGG ACCGAAATAG CCGGCAGCCC 
AGCAACCTGC CAGGCACTGT GGCCGTGCAC ATTCTCAGCA GCACCTCCCT CATGGCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543527.1
CDS	187..2229
Translation	MSCTGTPSCIYLLSALSLQDLVLDWEPRGVPPPLPGAAATMFSCVKPYEDQNYSALRRDCRRRKVLFEDPLFPATDDSLYYKGTPGPAVRWKRPKGICEDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKPNAYAGIFHFHFWRFGEWVDVVIDDRLPTVNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLGHGLLAFFKSEKLDMIRLRNPWGEREWNGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVINTSHLSIHKTWEEARLHGAWTLHEDPRQNRGGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYINSRSVFLRTDQPEGRYVIIPTTFEPGHTGEFLLRVFTDVPSNCRELRLDEPPHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTGANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKADPDNLQALHTLHLRDRNSRQPSNLPGTVAVHILSSTSLMAV

Target ORF information:

RefSeq Version	XM_011545225.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 5 (CAPN5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545225.1

ORF Insert Sequence:

ATGTCCTGCA CAGGGACGCC CAGCTGTATC TACCTGCTGT CAGCACTTTC TCTGCAGGAC 
CTGGTGCTGG ACTGGGAGCC CCGAGGTGTT CCCCCTCCCC TCCCTGGGGC AGCAGCCACC 
ATGTTCTCGT GTGTGAAGCC CTATGAGGAC CAGAACTACT CAGCCCTGAG GCGGGACTGC 
CGGCGCAGGA AGGTGCTCTT CGAGGACCCC CTCTTCCCCG CCACTGACGA CTCACTCTAC 
TATAAGGGCA CGCCGGGGCC CGCCGTCAGG TGGAAGCGAC CCAAGGGCAT CTGCGAGGAC 
CCCCGCCTCT TTGTGGATGG CATCAGCTCC CACGACCTGC ACCAGGGCCA GGTGGGCAAC 
TGCTGGTTTG TGGCAGCCTG CTCGTCACTT GCCTCCCGGG AGTCGCTGTG GCAAAAGGTC 
ATCCCAGACT GGAAGGAGCA GGAATGGGAC CCCGAAAAGC CCAACGCCTA CGCGGGCATC 
TTCCACTTCC ACTTCTGGCG CTTCGGGGAA TGGGTGGACG TGGTCATCGA TGACCGGCTG 
CCCACAGTCA ACAACCAGCT CATCTACTGC CACTCCAACT CCCGCAATGA GTTTTGGTGC 
GCCCTAGTGG AGAAGGCCTA TGCCAAACTG GCAGGCTGTT ACCAGGCCCT GGATGGAGGC 
AACACAGCAG ACGCACTGGT GGACTTCACG GGTGGTGTTT CTGAGCCCAT CGACCTGACC 
GAGGGTGACT TTGCCAACGA TGAGACTAAG AGGAACCAGC TCTTTGAGCG CATGTTAAAG 
GTGCACAGCC GGGGCGGCCT CATCAGTGCC TCCATCAAGG CAGTGACAGC AGCTGACATG 
GAGGCCCGCC TGGCGTGCGG CCTGGTAAAG GGCCACGCAT ACGCCGTCAC TGATGTGCGC 
AAGGTGCGCC TGGGCCACGG CCTACTGGCC TTCTTCAAGT CAGAGAAGTT GGACATGATC 
CGCCTGCGCA ACCCCTGGGG CGAGCGGGAG TGGAACGGGC CCTGGAGTGA CACCTCGGAG 
GAGTGGCAGA AAGTGAGCAA GAGTGAGCGG GAGAAGATGG GTGTGACCGT GCAGGACGAC 
GGTGAGTTCT GGATGACCTT CGAGGACGTG TGCCGGTACT TCACGGACAT CATCAAGTGC 
CGCGTGATCA ACACATCCCA CCTGAGCATC CACAAGACGT GGGAGGAGGC CCGGCTGCAT 
GGCGCCTGGA CGCTGCATGA GGACCCGCGA CAGAACCGCG GTGGCGGCTG CATCAACCAC 
AAGGACACCT TCTTCCAGAA CCCACAGTAC ATCTTCGAAG TCAAGAAGCC AGAAGATGAA 
GTCCTGATCT GCATCCAGCA GCGGCCAAAG CGGTCTACGC GCCGGGAGGG CAAGGGTGAG 
AACCTGGCCA TTGGCTTTGA CATCTACAAG GTGGAGGAGA ACCGCCAGTA CCGCATGCAC 
AGCCTGCAGC ACAAGGCCGC CAGCTCCATC TACATCAACT CACGCAGCGT CTTCCTGCGC 
ACCGACCAGC CCGAGGGCCG CTATGTCATC ATCCCCACAA CCTTCGAGCC AGGCCACACT 
GGCGAGTTCC TGCTCCGAGT CTTCACTGAT GTGCCCTCCA ACTGCCGGGA GCTGCGCCTG 
GATGAGCCCC CACACACCTG CTGGAGCTCC CTCTGTGGCT ACCCCCAGCT GGTGACCCAG 
GTACATGTCC TGGGAGCTGC TGGCCTCAAG GACTCCCCAA CAGGGGCTAA CTCTTATGTG 
ATCATCAAGT GTGAGGGAGA CAAAGTCCGC TCGGCTGTGC AGAAGGGCAC CTCCACACCA 
GAGTACAATG TGAAAGGCAT CTTCTACCGC AAGAAGCTGA GCCAGCCCAT CACTGTACAG 
GTCTGGAACC ACCGAGTGCT GAAGGATGAA TTTCTGGGCC AGGTGCACCT AAAGGCTGAC 
CCGGACAACC TCCAGGCCCT GCATACCCTC CACCTCCGGG ACCGAAATAG CCGGCAGCCC 
AGCAACCTGC CAGGCACTGT GGCCGTGCAC ATTCTCAGCA GCACCTCCCT CATGGCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN5 ( XM_017018223.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873712.1 Homo sapiens calpain 5 (CAPN5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu92471
Clone ID Related Accession (Same CDS sequence)	XM_017018223.1
Accession Version	XM_017018223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpain-5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATTC TGAAGCACAA AGAGGTTAAC TGCTTTGCAC CTCGCTGGCC TCAGTCTCCC 
CATCTGAGGA CGGGTGTTCC CCCTCCCCTC CCTGGGGCAG CAGCCACCAT GTTCTCGTGT 
GTGAAGCCCT ATGAGGACCA GAACTACTCA GCCCTGAGGC GGGACTGCCG GCGCAGGAAG 
GTGCTCTTCG AGGACCCCCT CTTCCCCGCC ACTGACGACT CACTCTACTA TAAGGGCACG 
CCGGGGCCCG CCGTCAGGTG GAAGCGACCC AAGGGCATCT GCGAGGACCC CCGCCTCTTT 
GTGGATGGCA TCAGCTCCCA CGACCTGCAC CAGGGCCAGG TGGGCAACTG CTGGTTTGTG 
GCAGCCTGCT CGTCACTTGC CTCCCGGGAG TCGCTGTGGC AAAAGGTCAT CCCAGACTGG 
AAGGAGCAGG AATGGGACCC CGAAAAGCCC AACGCCTACG CGGGCATCTT CCACTTCCAC 
TTCTGGCGCT TCGGGGAATG GGTGGACGTG GTCATCGATG ACCGGCTGCC CACAGTCAAC 
AACCAGCTCA TCTACTGCCA CTCCAACTCC CGCAATGAGT TTTGGTGCGC CCTAGTGGAG 
AAGGCCTATG CCAAACTGGC AGGCTGTTAC CAGGCCCTGG ATGGAGGCAA CACAGCAGAC 
GCACTGGTGG ACTTCACGGG TGGTGTTTCT GAGCCCATCG ACCTGACCGA GGGTGACTTT 
GCCAACGATG AGACTAAGAG GAACCAGCTC TTTGAGCGCA TGTTAAAGGT GCACAGCCGG 
GGCGGCCTCA TCAGTGCCTC CATCAAGGCA GTGACAGCAG CTGACATGGA GGCCCGCCTG 
GCGTGCGGCC TGGTAAAGGG CCACGCATAC GCCGTCACTG ATGTGCGCAA GGTGCGCCTG 
GGCCACGGCC TACTGGCCTT CTTCAAGTCA GAGAAGTTGG ACATGATCCG CCTGCGCAAC 
CCCTGGGGCG AGCGGGAGTG GAACGGGCCC TGGAGTGACA CCTCGGAGGA GTGGCAGAAA 
GTGAGCAAGA GTGAGCGGGA GAAGATGGGT GTGACCGTGC AGGACGACGG TGAGTTCTGG 
ATGACCTTCG AGGACGTGTG CCGGTACTTC ACGGACATCA TCAAGTGCCG CGTGATCAAC 
ACATCCCACC TGAGCATCCA CAAGACGTGG GAGGAGGCCC GGCTGCATGG CGCCTGGACG 
CTGCATGAGG ACCCGCGACA GAACCGCGGT GGCGGCTGCA TCAACCACAA GGACACCTTC 
TTCCAGAACC CACAGTACAT CTTCGAAGTC AAGAAGCCAG AAGATGAAGT CCTGATCTGC 
ATCCAGCAGC GGCCAAAGCG GTCTACGCGC CGGGAGGGCA AGGGTGAGAA CCTGGCCATT 
GGCTTTGACA TCTACAAGGT GGAGGAGAAC CGCCAGTACC GCATGCACAG CCTGCAGCAC 
AAGGCCGCCA GCTCCATCTA CATCAACTCA CGCAGCGTCT TCCTGCGCAC CGACCAGCCC 
GAGGGCCGCT ATGTCATCAT CCCCACAACC TTCGAGCCAG GCCACACTGG CGAGTTCCTG 
CTCCGAGTCT TCACTGATGT GCCCTCCAAC TGCCGGGAGC TGCGCCTGGA TGAGCCCCCA 
CACACCTGCT GGAGCTCCCT CTGTGGCTAC CCCCAGCTGG TGACCCAGGT ACATGTCCTG 
GGAGCTGCTG GCCTCAAGGA CTCCCCAACA GGGGCTAACT CTTATGTGAT CATCAAGTGT 
GAGGGAGACA AAGTCCGCTC GGCTGTGCAG AAGGGCACCT CCACACCAGA GTACAATGTG 
AAAGGCATCT TCTACCGCAA GAAGCTGAGC CAGCCCATCA CTGTACAGGT CTGGAACCAC 
CGAGTGCTGA AGGATGAATT TCTGGGCCAG GTGCACCTAA AGGCTGACCC GGACAACCTC 
CAGGCCCTGC ATACCCTCCA CCTCCGGGAC CGAAATAGCC GGCAGCCCAG CAACCTGCCA 
GGCACTGTGG CCGTGCACAT TCTCAGCAGC ACCTCCCTCA TGGCTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873712.1
CDS	1..2031
Translation	MRILKHKEVNCFAPRWPQSPHLRTGVPPPLPGAAATMFSCVKPYEDQNYSALRRDCRRRKVLFEDPLFPATDDSLYYKGTPGPAVRWKRPKGICEDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKPNAYAGIFHFHFWRFGEWVDVVIDDRLPTVNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLGHGLLAFFKSEKLDMIRLRNPWGEREWNGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVINTSHLSIHKTWEEARLHGAWTLHEDPRQNRGGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYINSRSVFLRTDQPEGRYVIIPTTFEPGHTGEFLLRVFTDVPSNCRELRLDEPPHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTGANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKADPDNLQALHTLHLRDRNSRQPSNLPGTVAVHILSSTSLMAV

Target ORF information:

RefSeq Version	XM_017018223.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 5 (CAPN5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018223.1

ORF Insert Sequence:

ATGAGAATTC TGAAGCACAA AGAGGTTAAC TGCTTTGCAC CTCGCTGGCC TCAGTCTCCC 
CATCTGAGGA CGGGTGTTCC CCCTCCCCTC CCTGGGGCAG CAGCCACCAT GTTCTCGTGT 
GTGAAGCCCT ATGAGGACCA GAACTACTCA GCCCTGAGGC GGGACTGCCG GCGCAGGAAG 
GTGCTCTTCG AGGACCCCCT CTTCCCCGCC ACTGACGACT CACTCTACTA TAAGGGCACG 
CCGGGGCCCG CCGTCAGGTG GAAGCGACCC AAGGGCATCT GCGAGGACCC CCGCCTCTTT 
GTGGATGGCA TCAGCTCCCA CGACCTGCAC CAGGGCCAGG TGGGCAACTG CTGGTTTGTG 
GCAGCCTGCT CGTCACTTGC CTCCCGGGAG TCGCTGTGGC AAAAGGTCAT CCCAGACTGG 
AAGGAGCAGG AATGGGACCC CGAAAAGCCC AACGCCTACG CGGGCATCTT CCACTTCCAC 
TTCTGGCGCT TCGGGGAATG GGTGGACGTG GTCATCGATG ACCGGCTGCC CACAGTCAAC 
AACCAGCTCA TCTACTGCCA CTCCAACTCC CGCAATGAGT TTTGGTGCGC CCTAGTGGAG 
AAGGCCTATG CCAAACTGGC AGGCTGTTAC CAGGCCCTGG ATGGAGGCAA CACAGCAGAC 
GCACTGGTGG ACTTCACGGG TGGTGTTTCT GAGCCCATCG ACCTGACCGA GGGTGACTTT 
GCCAACGATG AGACTAAGAG GAACCAGCTC TTTGAGCGCA TGTTAAAGGT GCACAGCCGG 
GGCGGCCTCA TCAGTGCCTC CATCAAGGCA GTGACAGCAG CTGACATGGA GGCCCGCCTG 
GCGTGCGGCC TGGTAAAGGG CCACGCATAC GCCGTCACTG ATGTGCGCAA GGTGCGCCTG 
GGCCACGGCC TACTGGCCTT CTTCAAGTCA GAGAAGTTGG ACATGATCCG CCTGCGCAAC 
CCCTGGGGCG AGCGGGAGTG GAACGGGCCC TGGAGTGACA CCTCGGAGGA GTGGCAGAAA 
GTGAGCAAGA GTGAGCGGGA GAAGATGGGT GTGACCGTGC AGGACGACGG TGAGTTCTGG 
ATGACCTTCG AGGACGTGTG CCGGTACTTC ACGGACATCA TCAAGTGCCG CGTGATCAAC 
ACATCCCACC TGAGCATCCA CAAGACGTGG GAGGAGGCCC GGCTGCATGG CGCCTGGACG 
CTGCATGAGG ACCCGCGACA GAACCGCGGT GGCGGCTGCA TCAACCACAA GGACACCTTC 
TTCCAGAACC CACAGTACAT CTTCGAAGTC AAGAAGCCAG AAGATGAAGT CCTGATCTGC 
ATCCAGCAGC GGCCAAAGCG GTCTACGCGC CGGGAGGGCA AGGGTGAGAA CCTGGCCATT 
GGCTTTGACA TCTACAAGGT GGAGGAGAAC CGCCAGTACC GCATGCACAG CCTGCAGCAC 
AAGGCCGCCA GCTCCATCTA CATCAACTCA CGCAGCGTCT TCCTGCGCAC CGACCAGCCC 
GAGGGCCGCT ATGTCATCAT CCCCACAACC TTCGAGCCAG GCCACACTGG CGAGTTCCTG 
CTCCGAGTCT TCACTGATGT GCCCTCCAAC TGCCGGGAGC TGCGCCTGGA TGAGCCCCCA 
CACACCTGCT GGAGCTCCCT CTGTGGCTAC CCCCAGCTGG TGACCCAGGT ACATGTCCTG 
GGAGCTGCTG GCCTCAAGGA CTCCCCAACA GGGGCTAACT CTTATGTGAT CATCAAGTGT 
GAGGGAGACA AAGTCCGCTC GGCTGTGCAG AAGGGCACCT CCACACCAGA GTACAATGTG 
AAAGGCATCT TCTACCGCAA GAAGCTGAGC CAGCCCATCA CTGTACAGGT CTGGAACCAC 
CGAGTGCTGA AGGATGAATT TCTGGGCCAG GTGCACCTAA AGGCTGACCC GGACAACCTC 
CAGGCCCTGC ATACCCTCCA CCTCCGGGAC CGAAATAGCC GGCAGCCCAG CAACCTGCCA 
GGCACTGTGG CCGTGCACAT TCTCAGCAGC ACCTCCCTCA TGGCTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Small-angle X-ray scattering of calpain-5 reveals a highly open conformation among calpains.
Journal of structural biology196(3)309-318(2016 Dec)
Gakhar L,Bassuk AG,Velez G,Khan S,Yang J,Tsang SH,Mahajan VB

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CAPN5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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