SLC12A2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC12A2
Entrez Gene ID	6558
Full Name	solute carrier family 12 member 2
Synonyms	BSC,BSC2,NKCC1,PPP1R141
General protein information	Preferred Names solute carrier family 12 member 2 Names solute carrier family 12 member 2 basolateral Na-K-Cl symporter bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 protein phosphatase 1, regulatory subunit 141 solute carrier family 12 (sodium/potassium/chloride transporter), member 2 solute carrier family 12 (sodium/potassium/chloride transporters), member 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q23.3
Summary	The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012].
Disorder MIM:	600840

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256461.2	NP_001243390.1	solute carrier family 12 member 2 isoform 2
NM_001256461.1	NP_001243390.1	solute carrier family 12 member 2 isoform 2
XM_011543588.2	XP_011541890.1	solute carrier family 12 member 2 isoform X1
XM_017009771.1	XP_016865260.1	solute carrier family 12 member 2 isoform X2
NM_001046.3	NP_001037.1	solute carrier family 12 member 2 isoform 1
NM_001046.2	NP_001037.1	solute carrier family 12 member 2 isoform 1

Pathways from BioSystems

Homologies

	SLC12A2	XP_001098624.1
Rattus norvegicus (Norway rat)	Slc12a2	NP_113986.1
Danio rerio (zebrafish)	slc12a2	NP_001002080.1
Drosophila melanogaster (fruit fly)	Ncc69	NP_648572.1
Homo sapiens (human)	SLC12A2	NP_001243390.1
Bos taurus (cattle)	SLC12A2	NP_777207.1
Gallus gallus (chicken)	SLC12A2	XP_004949435.1
Pan troglodytes (chimpanzee)	SLC12A2	XP_003310846.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001557	XP_321556.3
Xenopus tropicalis (tropical clawed frog)	slc12a2	XP_004910531.1
Canis lupus familiaris (dog)	SLC12A2	XP_538611.5
Mus musculus (house mouse)	Slc12a2	NP_033220.2

Gene Ontology
Bibliography

Related articles in PubMed

NKCC1 Regulates Migration Ability of Glioblastoma Cells by Modulation of Actin Dynamics and Interacting with Cofilin.
Schiapparelli P, Guerrero-Cazares H, Maga?a-Maldonado R, Hamilla SM, Ganaha S, Goulin Lippi Fernandes E, Huang CH, Aranda-Espinoza H, Devreotes P, Quinones-Hinojosa A
EBioMedicine2194-103(2017 Jul)

A High-Throughput Screening Assay for NKCC1 Cotransporter Using Nonradioactive Rubidium Flux Technology.
Gill S, Gill R, Wen Y, Enderle T, Roth D, Liang D
Assay and drug development technologies15(4)167-177(2017 May/Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we introduce the molecular mechanism by which flavonoids, specifically quercetin, act through elevation of [Cl(-) ]c via activation of NKCC1 on important factors controlling various body and cellular functions, such as (1) antihypertensive actions controlling blood volume dependent on the amounts of renal Na(+) reabsorption via expression of the epithelial Na(+) channel
Title: Actions of quercetin, a flavonoid, on ion transporters: its physiological roles.

We also discovered and replicated three genome-wide significant variants in previously unreported loci for RDW (SLC12A2 rs17764730, PSMB5 rs941718), and hematocrit (PROX1 rs3754140) and an upstream anti-sense long-noncoding RNA, LINC01184, as the likely causal variant
Title: Genome-wide association study of red blood cell traits in Hispanics/Latinos: The Hispanic Community Health Study/Study of Latinos.

NKCC1 and KCCs are coordinately regulated by L-WNK1 isoforms.
Title: With no lysine L-WNK1 isoforms are negative regulators of the K+-Cl- cotransporters.

Study describes a functional missense variant in SLC12A2 in human schizophrenia, and suggest that genetically encoded dysregulation of NKCC1 may be a risk factor for, or contribute to the pathogenesis of, schizophrenia
Title: Gain-of-function missense variant in SLC12A2, encoding the bumetanide-sensitive NKCC1 cotransporter, identified in human schizophrenia.

NKCC1 high expression predicted a bad clinical outcome for lung adenocarcinoma patients and EGFR-mutated subgroup. Therefore, NKCC1 may play a role in lung adenocarcinoma and novel therapeutic tactics could be developed by targeting NKCC1 protein.
Title: Expression of Na+-K+-2Cl- cotransporter isoform 1 (NKCC1) predicts poor prognosis in lung adenocarcinoma and EGFR-mutated adenocarcinoma patients.

The following SLC12A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28182	NM_001046.3 Latest version!	Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$609.50-$853.30 $1219.00
OHu96019	XM_017009771.1 Latest version!	Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OHu28209	NM_001256461.2 Latest version!	Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu60598	XM_011543588.2 Latest version!	Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OHu28209	NM_001256461.1	Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu28182	NM_001046.2	Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$609.50-$853.30 $1219.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC12A2 ( NM_001046.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001037.1 Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28182
Clone ID Related Accession (Same CDS sequence)	NM_001046.3 , NM_001046.2
Accession Version	NM_001046.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011332.8, AY280459.1, U30246.1, BC033003.2, AK025062.1, AA425619.1, AA425438.1, AA843086.1, AV708692.2 and BM978607.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001046.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY280459.1, SRR1660803.193764.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262461.7/ ENSP00000262461.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCCGC GGCCCACGGC GCCCTCCTCC GGCGCCCCGG GACTGGCCGG GGTCGGGGAG 
ACGCCGTCAG CCGCTGCGCT GGCCGCAGCC AGGGTGGAAC TGCCCGGCAC GGCTGTGCCC 
TCGGTGCCGG AGGATGCTGC GCCCGCGAGC CGGGACGGCG GCGGGGTCCG CGATGAGGGC 
CCCGCGGCGG CCGGGGACGG GCTGGGCAGA CCCTTGGGGC CCACCCCGAG CCAGAGCCGT 
TTCCAGGTGG ACCTGGTTTC CGAGAACGCC GGGCGGGCCG CTGCTGCGGC GGCGGCGGCG 
GCGGCGGCAG CGGCGGCGGC TGGTGCTGGG GCGGGGGCCA AGCAGACCCC CGCGGACGGG 
GAAGCCAGCG GCGAGAGCGA GCCGGCTAAA GGCAGCGAGG AAGCCAAGGG CCGCTTCCGC 
GTGAACTTCG TGGACCCAGC TGCCTCCTCG TCGGCTGAAG ACAGCCTGTC AGATGCTGCC 
GGGGTCGGAG TCGACGGGCC CAACGTGAGC TTCCAGAACG GCGGGGACAC GGTGCTGAGC 
GAGGGCAGCA GCCTGCACTC CGGCGGCGGC GGCGGCAGTG GGCACCACCA GCACTACTAT 
TATGATACCC ACACCAACAC CTACTACCTG CGCACCTTCG GCCACAACAC CATGGACGCT 
GTGCCCAGGA TCGATCACTA CCGGCACACA GCCGCGCAGC TGGGCGAGAA GCTGCTCCGG 
CCTAGCCTGG CGGAGCTCCA CGACGAGCTG GAAAAGGAAC CTTTTGAGGA TGGCTTTGCA 
AATGGGGAAG AAAGTACTCC AACCAGAGAT GCTGTGGTCA CGTATACTGC AGAAAGTAAA 
GGAGTCGTGA AGTTTGGCTG GATCAAGGGT GTATTAGTAC GTTGTATGTT AAACATTTGG 
GGTGTGATGC TTTTCATTAG ATTGTCATGG ATTGTGGGTC AAGCTGGAAT AGGTCTATCA 
GTCCTTGTAA TAATGATGGC CACTGTTGTG ACAACTATCA CAGGATTGTC TACTTCAGCA 
ATAGCAACTA ATGGATTTGT AAGAGGAGGA GGAGCATATT ATTTAATATC TAGAAGTCTA 
GGGCCAGAAT TTGGTGGTGC AATTGGTCTA ATCTTCGCCT TTGCCAACGC TGTTGCAGTT 
GCTATGTATG TGGTTGGATT TGCAGAAACC GTGGTGGAGT TGCTTAAGGA ACATTCCATA 
CTTATGATAG ATGAAATCAA TGATATCCGA ATTATTGGAG CCATTACAGT CGTGATTCTT 
TTAGGTATCT CAGTAGCTGG AATGGAGTGG GAAGCAAAAG CTCAGATTGT TCTTTTGGTG 
ATCCTACTTC TTGCTATTGG TGATTTCGTC ATAGGAACAT TTATCCCACT GGAGAGCAAG 
AAGCCAAAAG GGTTTTTTGG TTATAAATCT GAAATATTTA ATGAGAACTT TGGGCCCGAT 
TTTCGAGAGG AAGAGACTTT CTTTTCTGTA TTTGCCATCT TTTTTCCTGC TGCAACTGGT 
ATTCTGGCTG GAGCAAATAT CTCAGGTGAT CTTGCAGATC CTCAGTCAGC CATACCCAAA 
GGAACACTCC TAGCCATTTT AATTACTACA TTGGTTTACG TAGGAATTGC AGTATCTGTA 
GGTTCTTGTG TTGTTCGAGA TGCCACTGGA AACGTTAATG ACACTATCGT AACAGAGCTA 
ACAAACTGTA CTTCTGCAGC CTGCAAATTA AACTTTGATT TTTCATCTTG TGAAAGCAGT 
CCTTGTTCCT ATGGCCTAAT GAACAACTTC CAGGTAATGA GTATGGTGTC AGGATTTACA 
CCACTAATTT CTGCAGGTAT ATTTTCAGCC ACTCTTTCTT CAGCATTAGC ATCCCTAGTG 
AGTGCTCCCA AAATATTTCA GGCTCTATGT AAGGACAACA TCTACCCAGC TTTCCAGATG 
TTTGCTAAAG GTTATGGGAA AAATAATGAA CCTCTTCGTG GCTACATCTT AACATTCTTA 
ATTGCACTTG GATTCATCTT AATTGCTGAA CTGAATGTTA TTGCACCAAT TATCTCAAAC 
TTCTTCCTTG CATCATATGC ATTGATCAAT TTTTCAGTAT TCCATGCATC ACTTGCAAAA 
TCTCCAGGAT GGCGTCCTGC ATTCAAATAC TACAACATGT GGATATCACT TCTTGGAGCA 
ATTCTTTGTT GCATAGTAAT GTTCGTCATT AACTGGTGGG CTGCATTGCT AACATATGTG 
ATAGTCCTTG GGCTGTATAT TTATGTTACC TACAAAAAAC CAGATGTGAA TTGGGGATCC 
TCTACACAAG CCCTGACTTA CCTGAATGCA CTGCAGCATT CAATTCGTCT TTCTGGAGTG 
GAAGACCACG TGAAAAACTT TAGGCCACAG TGTCTTGTTA TGACAGGTGC TCCAAACTCA 
CGTCCAGCTT TACTTCATCT TGTTCATGAT TTCACAAAAA ATGTTGGTTT GATGATCTGT 
GGCCATGTAC ATATGGGTCC TCGAAGACAA GCCATGAAAG AGATGTCCAT CGATCAAGCC 
AAATATCAGC GATGGCTTAT TAAGAACAAA ATGAAGGCAT TTTATGCTCC AGTACATGCA 
GATGACTTGA GAGAAGGTGC ACAGTATTTG ATGCAGGCTG CTGGTCTTGG TCGTATGAAG 
CCAAACACAC TTGTCCTTGG ATTTAAGAAA GATTGGTTGC AAGCAGATAT GAGGGATGTG 
GATATGTATA TAAACTTATT TCATGATGCT TTTGACATAC AATATGGAGT AGTGGTTATT 
CGCCTAAAAG AAGGTCTGGA TATATCTCAT CTTCAAGGAC AAGAAGAATT ATTGTCATCA 
CAAGAGAAAT CTCCTGGCAC CAAGGATGTG GTAGTAAGTG TGGAATATAG TAAAAAGTCC 
GATTTAGATA CTTCCAAACC ACTCAGTGAA AAACCAATTA CACACAAAGT TGAGGAAGAG 
GATGGCAAGA CTGCAACTCA ACCACTGTTG AAAAAAGAAT CCAAAGGCCC TATTGTGCCT 
TTAAATGTAG CTGACCAAAA GCTTCTTGAA GCTAGTACAC AGTTTCAGAA AAAACAAGGA 
AAGAATACTA TTGATGTCTG GTGGCTTTTT GATGATGGAG GTTTGACCTT ATTGATACCT 
TACCTTCTGA CGACCAAGAA AAAATGGAAA GACTGTAAGA TCAGAGTATT CATTGGTGGA 
AAGATAAACA GAATAGACCA TGACCGGAGA GCGATGGCTA CTTTGCTTAG CAAGTTCCGG 
ATAGACTTTT CTGATATCAT GGTTCTAGGA GATATCAATA CCAAACCAAA GAAAGAAAAT 
ATTATAGCTT TTGAGGAAAT CATTGAGCCA TACAGACTTC ATGAAGATGA TAAAGAGCAA 
GATATTGCAG ATAAAATGAA AGAAGATGAA CCATGGCGAA TAACAGATAA TGAGCTTGAA 
CTTTATAAGA CCAAGACATA CCGGCAGATC AGGTTAAATG AGTTATTAAA GGAACATTCA 
AGCACAGCTA ATATTATTGT CATGAGTCTC CCAGTTGCAC GAAAAGGTGC TGTGTCTAGT 
GCTCTCTACA TGGCATGGTT AGAAGCTCTA TCTAAGGACC TACCACCAAT CCTCCTAGTT 
CGTGGGAATC ATCAGAGTGT CCTTACCTTC TATTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037.1
CDS	190..3828
Translation	MEPRPTAPSSGAPGLAGVGETPSAAALAAARVELPGTAVPSVPEDAAPASRDGGGVRDEGPAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAAAAAAAAAAAGAGAGAKQTPADGEASGESEPAKGSEEAKGRFRVNFVDPAASSSAEDSLSDAAGVGVDGPNVSFQNGGDTVLSEGSSLHSGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFIPLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	NM_001046.3
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001046.3

ORF Insert Sequence:

ATGGAGCCGC GGCCCACGGC GCCCTCCTCC GGCGCCCCGG GACTGGCCGG GGTCGGGGAG 
ACGCCGTCAG CCGCTGCGCT GGCCGCAGCC AGGGTGGAAC TGCCCGGCAC GGCTGTGCCC 
TCGGTGCCGG AGGATGCTGC GCCCGCGAGC CGGGACGGCG GCGGGGTCCG CGATGAGGGC 
CCCGCGGCGG CCGGGGACGG GCTGGGCAGA CCCTTGGGGC CCACCCCGAG CCAGAGCCGT 
TTCCAGGTGG ACCTGGTTTC CGAGAACGCC GGGCGGGCCG CTGCTGCGGC GGCGGCGGCG 
GCGGCGGCAG CGGCGGCGGC TGGTGCTGGG GCGGGGGCCA AGCAGACCCC CGCGGACGGG 
GAAGCCAGCG GCGAGAGCGA GCCGGCTAAA GGCAGCGAGG AAGCCAAGGG CCGCTTCCGC 
GTGAACTTCG TGGACCCAGC TGCCTCCTCG TCGGCTGAAG ACAGCCTGTC AGATGCTGCC 
GGGGTCGGAG TCGACGGGCC CAACGTGAGC TTCCAGAACG GCGGGGACAC GGTGCTGAGC 
GAGGGCAGCA GCCTGCACTC CGGCGGCGGC GGCGGCAGTG GGCACCACCA GCACTACTAT 
TATGATACCC ACACCAACAC CTACTACCTG CGCACCTTCG GCCACAACAC CATGGACGCT 
GTGCCCAGGA TCGATCACTA CCGGCACACA GCCGCGCAGC TGGGCGAGAA GCTGCTCCGG 
CCTAGCCTGG CGGAGCTCCA CGACGAGCTG GAAAAGGAAC CTTTTGAGGA TGGCTTTGCA 
AATGGGGAAG AAAGTACTCC AACCAGAGAT GCTGTGGTCA CGTATACTGC AGAAAGTAAA 
GGAGTCGTGA AGTTTGGCTG GATCAAGGGT GTATTAGTAC GTTGTATGTT AAACATTTGG 
GGTGTGATGC TTTTCATTAG ATTGTCATGG ATTGTGGGTC AAGCTGGAAT AGGTCTATCA 
GTCCTTGTAA TAATGATGGC CACTGTTGTG ACAACTATCA CAGGATTGTC TACTTCAGCA 
ATAGCAACTA ATGGATTTGT AAGAGGAGGA GGAGCATATT ATTTAATATC TAGAAGTCTA 
GGGCCAGAAT TTGGTGGTGC AATTGGTCTA ATCTTCGCCT TTGCCAACGC TGTTGCAGTT 
GCTATGTATG TGGTTGGATT TGCAGAAACC GTGGTGGAGT TGCTTAAGGA ACATTCCATA 
CTTATGATAG ATGAAATCAA TGATATCCGA ATTATTGGAG CCATTACAGT CGTGATTCTT 
TTAGGTATCT CAGTAGCTGG AATGGAGTGG GAAGCAAAAG CTCAGATTGT TCTTTTGGTG 
ATCCTACTTC TTGCTATTGG TGATTTCGTC ATAGGAACAT TTATCCCACT GGAGAGCAAG 
AAGCCAAAAG GGTTTTTTGG TTATAAATCT GAAATATTTA ATGAGAACTT TGGGCCCGAT 
TTTCGAGAGG AAGAGACTTT CTTTTCTGTA TTTGCCATCT TTTTTCCTGC TGCAACTGGT 
ATTCTGGCTG GAGCAAATAT CTCAGGTGAT CTTGCAGATC CTCAGTCAGC CATACCCAAA 
GGAACACTCC TAGCCATTTT AATTACTACA TTGGTTTACG TAGGAATTGC AGTATCTGTA 
GGTTCTTGTG TTGTTCGAGA TGCCACTGGA AACGTTAATG ACACTATCGT AACAGAGCTA 
ACAAACTGTA CTTCTGCAGC CTGCAAATTA AACTTTGATT TTTCATCTTG TGAAAGCAGT 
CCTTGTTCCT ATGGCCTAAT GAACAACTTC CAGGTAATGA GTATGGTGTC AGGATTTACA 
CCACTAATTT CTGCAGGTAT ATTTTCAGCC ACTCTTTCTT CAGCATTAGC ATCCCTAGTG 
AGTGCTCCCA AAATATTTCA GGCTCTATGT AAGGACAACA TCTACCCAGC TTTCCAGATG 
TTTGCTAAAG GTTATGGGAA AAATAATGAA CCTCTTCGTG GCTACATCTT AACATTCTTA 
ATTGCACTTG GATTCATCTT AATTGCTGAA CTGAATGTTA TTGCACCAAT TATCTCAAAC 
TTCTTCCTTG CATCATATGC ATTGATCAAT TTTTCAGTAT TCCATGCATC ACTTGCAAAA 
TCTCCAGGAT GGCGTCCTGC ATTCAAATAC TACAACATGT GGATATCACT TCTTGGAGCA 
ATTCTTTGTT GCATAGTAAT GTTCGTCATT AACTGGTGGG CTGCATTGCT AACATATGTG 
ATAGTCCTTG GGCTGTATAT TTATGTTACC TACAAAAAAC CAGATGTGAA TTGGGGATCC 
TCTACACAAG CCCTGACTTA CCTGAATGCA CTGCAGCATT CAATTCGTCT TTCTGGAGTG 
GAAGACCACG TGAAAAACTT TAGGCCACAG TGTCTTGTTA TGACAGGTGC TCCAAACTCA 
CGTCCAGCTT TACTTCATCT TGTTCATGAT TTCACAAAAA ATGTTGGTTT GATGATCTGT 
GGCCATGTAC ATATGGGTCC TCGAAGACAA GCCATGAAAG AGATGTCCAT CGATCAAGCC 
AAATATCAGC GATGGCTTAT TAAGAACAAA ATGAAGGCAT TTTATGCTCC AGTACATGCA 
GATGACTTGA GAGAAGGTGC ACAGTATTTG ATGCAGGCTG CTGGTCTTGG TCGTATGAAG 
CCAAACACAC TTGTCCTTGG ATTTAAGAAA GATTGGTTGC AAGCAGATAT GAGGGATGTG 
GATATGTATA TAAACTTATT TCATGATGCT TTTGACATAC AATATGGAGT AGTGGTTATT 
CGCCTAAAAG AAGGTCTGGA TATATCTCAT CTTCAAGGAC AAGAAGAATT ATTGTCATCA 
CAAGAGAAAT CTCCTGGCAC CAAGGATGTG GTAGTAAGTG TGGAATATAG TAAAAAGTCC 
GATTTAGATA CTTCCAAACC ACTCAGTGAA AAACCAATTA CACACAAAGT TGAGGAAGAG 
GATGGCAAGA CTGCAACTCA ACCACTGTTG AAAAAAGAAT CCAAAGGCCC TATTGTGCCT 
TTAAATGTAG CTGACCAAAA GCTTCTTGAA GCTAGTACAC AGTTTCAGAA AAAACAAGGA 
AAGAATACTA TTGATGTCTG GTGGCTTTTT GATGATGGAG GTTTGACCTT ATTGATACCT 
TACCTTCTGA CGACCAAGAA AAAATGGAAA GACTGTAAGA TCAGAGTATT CATTGGTGGA 
AAGATAAACA GAATAGACCA TGACCGGAGA GCGATGGCTA CTTTGCTTAG CAAGTTCCGG 
ATAGACTTTT CTGATATCAT GGTTCTAGGA GATATCAATA CCAAACCAAA GAAAGAAAAT 
ATTATAGCTT TTGAGGAAAT CATTGAGCCA TACAGACTTC ATGAAGATGA TAAAGAGCAA 
GATATTGCAG ATAAAATGAA AGAAGATGAA CCATGGCGAA TAACAGATAA TGAGCTTGAA 
CTTTATAAGA CCAAGACATA CCGGCAGATC AGGTTAAATG AGTTATTAAA GGAACATTCA 
AGCACAGCTA ATATTATTGT CATGAGTCTC CCAGTTGCAC GAAAAGGTGC TGTGTCTAGT 
GCTCTCTACA TGGCATGGTT AGAAGCTCTA TCTAAGGACC TACCACCAAT CCTCCTAGTT 
CGTGGGAATC ATCAGAGTGT CCTTACCTTC TATTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A2 ( NM_001046.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001037.1 Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28182
Clone ID Related Accession (Same CDS sequence)	NM_001046.3 , NM_001046.2
Accession Version	NM_001046.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY280459.1, U30246.1, BC033003.2, AK025062.1, AA425619.1, AA425438.1, AA843086.1, AV708692.2, BM978607.1 and AI493213.1. This sequence is a reference standard in the RefSeqGene project. On Dec 1, 2003 this sequence version replaced NM_001046.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U30246.1, SRR1660803.193764.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCGC GGCCCACGGC GCCCTCCTCC GGCGCCCCGG GACTGGCCGG GGTCGGGGAG 
ACGCCGTCAG CCGCTGCGCT GGCCGCAGCC AGGGTGGAAC TGCCCGGCAC GGCTGTGCCC 
TCGGTGCCGG AGGATGCTGC GCCCGCGAGC CGGGACGGCG GCGGGGTCCG CGATGAGGGC 
CCCGCGGCGG CCGGGGACGG GCTGGGCAGA CCCTTGGGGC CCACCCCGAG CCAGAGCCGT 
TTCCAGGTGG ACCTGGTTTC CGAGAACGCC GGGCGGGCCG CTGCTGCGGC GGCGGCGGCG 
GCGGCGGCAG CGGCGGCGGC TGGTGCTGGG GCGGGGGCCA AGCAGACCCC CGCGGACGGG 
GAAGCCAGCG GCGAGAGCGA GCCGGCTAAA GGCAGCGAGG AAGCCAAGGG CCGCTTCCGC 
GTGAACTTCG TGGACCCAGC TGCCTCCTCG TCGGCTGAAG ACAGCCTGTC AGATGCTGCC 
GGGGTCGGAG TCGACGGGCC CAACGTGAGC TTCCAGAACG GCGGGGACAC GGTGCTGAGC 
GAGGGCAGCA GCCTGCACTC CGGCGGCGGC GGCGGCAGTG GGCACCACCA GCACTACTAT 
TATGATACCC ACACCAACAC CTACTACCTG CGCACCTTCG GCCACAACAC CATGGACGCT 
GTGCCCAGGA TCGATCACTA CCGGCACACA GCCGCGCAGC TGGGCGAGAA GCTGCTCCGG 
CCTAGCCTGG CGGAGCTCCA CGACGAGCTG GAAAAGGAAC CTTTTGAGGA TGGCTTTGCA 
AATGGGGAAG AAAGTACTCC AACCAGAGAT GCTGTGGTCA CGTATACTGC AGAAAGTAAA 
GGAGTCGTGA AGTTTGGCTG GATCAAGGGT GTATTAGTAC GTTGTATGTT AAACATTTGG 
GGTGTGATGC TTTTCATTAG ATTGTCATGG ATTGTGGGTC AAGCTGGAAT AGGTCTATCA 
GTCCTTGTAA TAATGATGGC CACTGTTGTG ACAACTATCA CAGGATTGTC TACTTCAGCA 
ATAGCAACTA ATGGATTTGT AAGAGGAGGA GGAGCATATT ATTTAATATC TAGAAGTCTA 
GGGCCAGAAT TTGGTGGTGC AATTGGTCTA ATCTTCGCCT TTGCCAACGC TGTTGCAGTT 
GCTATGTATG TGGTTGGATT TGCAGAAACC GTGGTGGAGT TGCTTAAGGA ACATTCCATA 
CTTATGATAG ATGAAATCAA TGATATCCGA ATTATTGGAG CCATTACAGT CGTGATTCTT 
TTAGGTATCT CAGTAGCTGG AATGGAGTGG GAAGCAAAAG CTCAGATTGT TCTTTTGGTG 
ATCCTACTTC TTGCTATTGG TGATTTCGTC ATAGGAACAT TTATCCCACT GGAGAGCAAG 
AAGCCAAAAG GGTTTTTTGG TTATAAATCT GAAATATTTA ATGAGAACTT TGGGCCCGAT 
TTTCGAGAGG AAGAGACTTT CTTTTCTGTA TTTGCCATCT TTTTTCCTGC TGCAACTGGT 
ATTCTGGCTG GAGCAAATAT CTCAGGTGAT CTTGCAGATC CTCAGTCAGC CATACCCAAA 
GGAACACTCC TAGCCATTTT AATTACTACA TTGGTTTACG TAGGAATTGC AGTATCTGTA 
GGTTCTTGTG TTGTTCGAGA TGCCACTGGA AACGTTAATG ACACTATCGT AACAGAGCTA 
ACAAACTGTA CTTCTGCAGC CTGCAAATTA AACTTTGATT TTTCATCTTG TGAAAGCAGT 
CCTTGTTCCT ATGGCCTAAT GAACAACTTC CAGGTAATGA GTATGGTGTC AGGATTTACA 
CCACTAATTT CTGCAGGTAT ATTTTCAGCC ACTCTTTCTT CAGCATTAGC ATCCCTAGTG 
AGTGCTCCCA AAATATTTCA GGCTCTATGT AAGGACAACA TCTACCCAGC TTTCCAGATG 
TTTGCTAAAG GTTATGGGAA AAATAATGAA CCTCTTCGTG GCTACATCTT AACATTCTTA 
ATTGCACTTG GATTCATCTT AATTGCTGAA CTGAATGTTA TTGCACCAAT TATCTCAAAC 
TTCTTCCTTG CATCATATGC ATTGATCAAT TTTTCAGTAT TCCATGCATC ACTTGCAAAA 
TCTCCAGGAT GGCGTCCTGC ATTCAAATAC TACAACATGT GGATATCACT TCTTGGAGCA 
ATTCTTTGTT GCATAGTAAT GTTCGTCATT AACTGGTGGG CTGCATTGCT AACATATGTG 
ATAGTCCTTG GGCTGTATAT TTATGTTACC TACAAAAAAC CAGATGTGAA TTGGGGATCC 
TCTACACAAG CCCTGACTTA CCTGAATGCA CTGCAGCATT CAATTCGTCT TTCTGGAGTG 
GAAGACCACG TGAAAAACTT TAGGCCACAG TGTCTTGTTA TGACAGGTGC TCCAAACTCA 
CGTCCAGCTT TACTTCATCT TGTTCATGAT TTCACAAAAA ATGTTGGTTT GATGATCTGT 
GGCCATGTAC ATATGGGTCC TCGAAGACAA GCCATGAAAG AGATGTCCAT CGATCAAGCC 
AAATATCAGC GATGGCTTAT TAAGAACAAA ATGAAGGCAT TTTATGCTCC AGTACATGCA 
GATGACTTGA GAGAAGGTGC ACAGTATTTG ATGCAGGCTG CTGGTCTTGG TCGTATGAAG 
CCAAACACAC TTGTCCTTGG ATTTAAGAAA GATTGGTTGC AAGCAGATAT GAGGGATGTG 
GATATGTATA TAAACTTATT TCATGATGCT TTTGACATAC AATATGGAGT AGTGGTTATT 
CGCCTAAAAG AAGGTCTGGA TATATCTCAT CTTCAAGGAC AAGAAGAATT ATTGTCATCA 
CAAGAGAAAT CTCCTGGCAC CAAGGATGTG GTAGTAAGTG TGGAATATAG TAAAAAGTCC 
GATTTAGATA CTTCCAAACC ACTCAGTGAA AAACCAATTA CACACAAAGT TGAGGAAGAG 
GATGGCAAGA CTGCAACTCA ACCACTGTTG AAAAAAGAAT CCAAAGGCCC TATTGTGCCT 
TTAAATGTAG CTGACCAAAA GCTTCTTGAA GCTAGTACAC AGTTTCAGAA AAAACAAGGA 
AAGAATACTA TTGATGTCTG GTGGCTTTTT GATGATGGAG GTTTGACCTT ATTGATACCT 
TACCTTCTGA CGACCAAGAA AAAATGGAAA GACTGTAAGA TCAGAGTATT CATTGGTGGA 
AAGATAAACA GAATAGACCA TGACCGGAGA GCGATGGCTA CTTTGCTTAG CAAGTTCCGG 
ATAGACTTTT CTGATATCAT GGTTCTAGGA GATATCAATA CCAAACCAAA GAAAGAAAAT 
ATTATAGCTT TTGAGGAAAT CATTGAGCCA TACAGACTTC ATGAAGATGA TAAAGAGCAA 
GATATTGCAG ATAAAATGAA AGAAGATGAA CCATGGCGAA TAACAGATAA TGAGCTTGAA 
CTTTATAAGA CCAAGACATA CCGGCAGATC AGGTTAAATG AGTTATTAAA GGAACATTCA 
AGCACAGCTA ATATTATTGT CATGAGTCTC CCAGTTGCAC GAAAAGGTGC TGTGTCTAGT 
GCTCTCTACA TGGCATGGTT AGAAGCTCTA TCTAAGGACC TACCACCAAT CCTCCTAGTT 
CGTGGGAATC ATCAGAGTGT CCTTACCTTC TATTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037.1
CDS	165..3803
Translation	MEPRPTAPSSGAPGLAGVGETPSAAALAAARVELPGTAVPSVPEDAAPASRDGGGVRDEGPAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAAAAAAAAAAAGAGAGAKQTPADGEASGESEPAKGSEEAKGRFRVNFVDPAASSSAEDSLSDAAGVGVDGPNVSFQNGGDTVLSEGSSLHSGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFIPLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	NM_001046.2
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001046.2

ORF Insert Sequence:

ATGGAGCCGC GGCCCACGGC GCCCTCCTCC GGCGCCCCGG GACTGGCCGG GGTCGGGGAG 
ACGCCGTCAG CCGCTGCGCT GGCCGCAGCC AGGGTGGAAC TGCCCGGCAC GGCTGTGCCC 
TCGGTGCCGG AGGATGCTGC GCCCGCGAGC CGGGACGGCG GCGGGGTCCG CGATGAGGGC 
CCCGCGGCGG CCGGGGACGG GCTGGGCAGA CCCTTGGGGC CCACCCCGAG CCAGAGCCGT 
TTCCAGGTGG ACCTGGTTTC CGAGAACGCC GGGCGGGCCG CTGCTGCGGC GGCGGCGGCG 
GCGGCGGCAG CGGCGGCGGC TGGTGCTGGG GCGGGGGCCA AGCAGACCCC CGCGGACGGG 
GAAGCCAGCG GCGAGAGCGA GCCGGCTAAA GGCAGCGAGG AAGCCAAGGG CCGCTTCCGC 
GTGAACTTCG TGGACCCAGC TGCCTCCTCG TCGGCTGAAG ACAGCCTGTC AGATGCTGCC 
GGGGTCGGAG TCGACGGGCC CAACGTGAGC TTCCAGAACG GCGGGGACAC GGTGCTGAGC 
GAGGGCAGCA GCCTGCACTC CGGCGGCGGC GGCGGCAGTG GGCACCACCA GCACTACTAT 
TATGATACCC ACACCAACAC CTACTACCTG CGCACCTTCG GCCACAACAC CATGGACGCT 
GTGCCCAGGA TCGATCACTA CCGGCACACA GCCGCGCAGC TGGGCGAGAA GCTGCTCCGG 
CCTAGCCTGG CGGAGCTCCA CGACGAGCTG GAAAAGGAAC CTTTTGAGGA TGGCTTTGCA 
AATGGGGAAG AAAGTACTCC AACCAGAGAT GCTGTGGTCA CGTATACTGC AGAAAGTAAA 
GGAGTCGTGA AGTTTGGCTG GATCAAGGGT GTATTAGTAC GTTGTATGTT AAACATTTGG 
GGTGTGATGC TTTTCATTAG ATTGTCATGG ATTGTGGGTC AAGCTGGAAT AGGTCTATCA 
GTCCTTGTAA TAATGATGGC CACTGTTGTG ACAACTATCA CAGGATTGTC TACTTCAGCA 
ATAGCAACTA ATGGATTTGT AAGAGGAGGA GGAGCATATT ATTTAATATC TAGAAGTCTA 
GGGCCAGAAT TTGGTGGTGC AATTGGTCTA ATCTTCGCCT TTGCCAACGC TGTTGCAGTT 
GCTATGTATG TGGTTGGATT TGCAGAAACC GTGGTGGAGT TGCTTAAGGA ACATTCCATA 
CTTATGATAG ATGAAATCAA TGATATCCGA ATTATTGGAG CCATTACAGT CGTGATTCTT 
TTAGGTATCT CAGTAGCTGG AATGGAGTGG GAAGCAAAAG CTCAGATTGT TCTTTTGGTG 
ATCCTACTTC TTGCTATTGG TGATTTCGTC ATAGGAACAT TTATCCCACT GGAGAGCAAG 
AAGCCAAAAG GGTTTTTTGG TTATAAATCT GAAATATTTA ATGAGAACTT TGGGCCCGAT 
TTTCGAGAGG AAGAGACTTT CTTTTCTGTA TTTGCCATCT TTTTTCCTGC TGCAACTGGT 
ATTCTGGCTG GAGCAAATAT CTCAGGTGAT CTTGCAGATC CTCAGTCAGC CATACCCAAA 
GGAACACTCC TAGCCATTTT AATTACTACA TTGGTTTACG TAGGAATTGC AGTATCTGTA 
GGTTCTTGTG TTGTTCGAGA TGCCACTGGA AACGTTAATG ACACTATCGT AACAGAGCTA 
ACAAACTGTA CTTCTGCAGC CTGCAAATTA AACTTTGATT TTTCATCTTG TGAAAGCAGT 
CCTTGTTCCT ATGGCCTAAT GAACAACTTC CAGGTAATGA GTATGGTGTC AGGATTTACA 
CCACTAATTT CTGCAGGTAT ATTTTCAGCC ACTCTTTCTT CAGCATTAGC ATCCCTAGTG 
AGTGCTCCCA AAATATTTCA GGCTCTATGT AAGGACAACA TCTACCCAGC TTTCCAGATG 
TTTGCTAAAG GTTATGGGAA AAATAATGAA CCTCTTCGTG GCTACATCTT AACATTCTTA 
ATTGCACTTG GATTCATCTT AATTGCTGAA CTGAATGTTA TTGCACCAAT TATCTCAAAC 
TTCTTCCTTG CATCATATGC ATTGATCAAT TTTTCAGTAT TCCATGCATC ACTTGCAAAA 
TCTCCAGGAT GGCGTCCTGC ATTCAAATAC TACAACATGT GGATATCACT TCTTGGAGCA 
ATTCTTTGTT GCATAGTAAT GTTCGTCATT AACTGGTGGG CTGCATTGCT AACATATGTG 
ATAGTCCTTG GGCTGTATAT TTATGTTACC TACAAAAAAC CAGATGTGAA TTGGGGATCC 
TCTACACAAG CCCTGACTTA CCTGAATGCA CTGCAGCATT CAATTCGTCT TTCTGGAGTG 
GAAGACCACG TGAAAAACTT TAGGCCACAG TGTCTTGTTA TGACAGGTGC TCCAAACTCA 
CGTCCAGCTT TACTTCATCT TGTTCATGAT TTCACAAAAA ATGTTGGTTT GATGATCTGT 
GGCCATGTAC ATATGGGTCC TCGAAGACAA GCCATGAAAG AGATGTCCAT CGATCAAGCC 
AAATATCAGC GATGGCTTAT TAAGAACAAA ATGAAGGCAT TTTATGCTCC AGTACATGCA 
GATGACTTGA GAGAAGGTGC ACAGTATTTG ATGCAGGCTG CTGGTCTTGG TCGTATGAAG 
CCAAACACAC TTGTCCTTGG ATTTAAGAAA GATTGGTTGC AAGCAGATAT GAGGGATGTG 
GATATGTATA TAAACTTATT TCATGATGCT TTTGACATAC AATATGGAGT AGTGGTTATT 
CGCCTAAAAG AAGGTCTGGA TATATCTCAT CTTCAAGGAC AAGAAGAATT ATTGTCATCA 
CAAGAGAAAT CTCCTGGCAC CAAGGATGTG GTAGTAAGTG TGGAATATAG TAAAAAGTCC 
GATTTAGATA CTTCCAAACC ACTCAGTGAA AAACCAATTA CACACAAAGT TGAGGAAGAG 
GATGGCAAGA CTGCAACTCA ACCACTGTTG AAAAAAGAAT CCAAAGGCCC TATTGTGCCT 
TTAAATGTAG CTGACCAAAA GCTTCTTGAA GCTAGTACAC AGTTTCAGAA AAAACAAGGA 
AAGAATACTA TTGATGTCTG GTGGCTTTTT GATGATGGAG GTTTGACCTT ATTGATACCT 
TACCTTCTGA CGACCAAGAA AAAATGGAAA GACTGTAAGA TCAGAGTATT CATTGGTGGA 
AAGATAAACA GAATAGACCA TGACCGGAGA GCGATGGCTA CTTTGCTTAG CAAGTTCCGG 
ATAGACTTTT CTGATATCAT GGTTCTAGGA GATATCAATA CCAAACCAAA GAAAGAAAAT 
ATTATAGCTT TTGAGGAAAT CATTGAGCCA TACAGACTTC ATGAAGATGA TAAAGAGCAA 
GATATTGCAG ATAAAATGAA AGAAGATGAA CCATGGCGAA TAACAGATAA TGAGCTTGAA 
CTTTATAAGA CCAAGACATA CCGGCAGATC AGGTTAAATG AGTTATTAAA GGAACATTCA 
AGCACAGCTA ATATTATTGT CATGAGTCTC CCAGTTGCAC GAAAAGGTGC TGTGTCTAGT 
GCTCTCTACA TGGCATGGTT AGAAGCTCTA TCTAAGGACC TACCACCAAT CCTCCTAGTT 
CGTGGGAATC ATCAGAGTGT CCTTACCTTC TATTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A2 ( XM_017009771.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865260.1 Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant X3, mRNA.

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CloneID	OHu96019
Clone ID Related Accession (Same CDS sequence)	XM_017009771.1
Accession Version	XM_017009771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAACT TCCAGGTAAT GAGTATGGTG TCAGGATTTA CACCACTAAT TTCTGCAGGT 
ATATTTTCAG CCACTCTTTC TTCAGCATTA GCATCCCTAG TGAGTGCTCC CAAAATATTT 
CAGGCTCTAT GTAAGGACAA CATCTACCCA GCTTTCCAGA TGTTTGCTAA AGGTTATGGG 
AAAAATAATG AACCTCTTCG TGGCTACATC TTAACATTCT TAATTGCACT TGGATTCATC 
TTAATTGCTG AACTGAATGT TATTGCACCA ATTATCTCAA ACTTCTTCCT TGCATCATAT 
GCATTGATCA ATTTTTCAGT ATTCCATGCA TCACTTGCAA AATCTCCAGG ATGGCGTCCT 
GCATTCAAAT ACTACAACAT GTGGATATCA CTTCTTGGAG CAATTCTTTG TTGCATAGTA 
ATGTTCGTCA TTAACTGGTG GGCTGCATTG CTAACATATG TGATAGTCCT TGGGCTGTAT 
ATTTATGTTA CCTACAAAAA ACCAGATGTG AATTGGGGAT CCTCTACACA AGCCCTGACT 
TACCTGAATG CACTGCAGCA TTCAATTCGT CTTTCTGGAG TGGAAGACCA CGTGAAAAAC 
TTTAGGCCAC AGTGTCTTGT TATGACAGGT GCTCCAAACT CACGTCCAGC TTTACTTCAT 
CTTGTTCATG ATTTCACAAA AAATGTTGGT TTGATGATCT GTGGCCATGT ACATATGGGT 
CCTCGAAGAC AAGCCATGAA AGAGATGTCC ATCGATCAAG CCAAATATCA GCGATGGCTT 
ATTAAGAACA AAATGAAGGC ATTTTATGCT CCAGTACATG CAGATGACTT GAGAGAAGGT 
GCACAGTATT TGATGCAGGC TGCTGGTCTT GGTCGTATGA AGCCAAACAC ACTTGTCCTT 
GGATTTAAGA AAGATTGGTT GCAAGCAGAT ATGAGGGATG TGGATATGTA TATAAACTTA 
TTTCATGATG CTTTTGACAT ACAATATGGA GTAGTGGTTA TTCGCCTAAA AGAAGGTCTG 
GATATATCTC ATCTTCAAGG ACAAGAAGAA TTATTGTCAT CACAAGAGAA ATCTCCTGGC 
ACCAAGGATG TGGTAGTAAG TGTGGAATAT AGTAAAAAGT CCGATTTAGA TACTTCCAAA 
CCACTCAGTG AAAAACCAAT TACACACAAA GTTGAGGAAG AGGATGGCAA GACTGCAACT 
CAACCACTGT TGAAAAAAGA ATCCAAAGGC CCTATTGTGC CTTTAAATGT AGCTGACCAA 
AAGCTTCTTG AAGCTAGTAC ACAGTTTCAG AAAAAACAAG GAAAGAATAC TATTGATGTC 
TGGTGGCTTT TTGATGATGG AGGTTTGACC TTATTGATAC CTTACCTTCT GACGACCAAG 
AAAAAATGGA AAGACTGTAA GATCAGAGTA TTCATTGGTG GAAAGATAAA CAGAATAGAC 
CATGACCGGA GAGCGATGGC TACTTTGCTT AGCAAGTTCC GGATAGACTT TTCTGATATC 
ATGGTTCTAG GAGATATCAA TACCAAACCA AAGAAAGAAA ATATTATAGC TTTTGAGGAA 
ATCATTGAGC CATACAGACT TCATGAAGAT GATAAAGAGC AAGATATTGC AGATAAAATG 
AAAGAAGATG AACCATGGCG AATAACAGAT AATGAGCTTG AACTTTATAA GACCAAGACA 
TACCGGCAGA TCAGGTTAAA TGAGTTATTA AAGGAACATT CAAGCACAGC TAATATTATT 
GTCATGAGTC TCCCAGTTGC ACGAAAAGGT GCTGTGTCTA GTGCTCTCTA CATGGCATGG 
TTAGAAGCTC TATCTAAGGA CCTACCACCA ATCCTCCTAG TTCGTGGGAA TCATCAGAGT 
GTCCTTACCT TCTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865260.1
CDS	1073..2953
Translation	MNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	XM_017009771.1
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009771.1

ORF Insert Sequence:

ATGAACAACT TCCAGGTAAT GAGTATGGTG TCAGGATTTA CACCACTAAT TTCTGCAGGT 
ATATTTTCAG CCACTCTTTC TTCAGCATTA GCATCCCTAG TGAGTGCTCC CAAAATATTT 
CAGGCTCTAT GTAAGGACAA CATCTACCCA GCTTTCCAGA TGTTTGCTAA AGGTTATGGG 
AAAAATAATG AACCTCTTCG TGGCTACATC TTAACATTCT TAATTGCACT TGGATTCATC 
TTAATTGCTG AACTGAATGT TATTGCACCA ATTATCTCAA ACTTCTTCCT TGCATCATAT 
GCATTGATCA ATTTTTCAGT ATTCCATGCA TCACTTGCAA AATCTCCAGG ATGGCGTCCT 
GCATTCAAAT ACTACAACAT GTGGATATCA CTTCTTGGAG CAATTCTTTG TTGCATAGTA 
ATGTTCGTCA TTAACTGGTG GGCTGCATTG CTAACATATG TGATAGTCCT TGGGCTGTAT 
ATTTATGTTA CCTACAAAAA ACCAGATGTG AATTGGGGAT CCTCTACACA AGCCCTGACT 
TACCTGAATG CACTGCAGCA TTCAATTCGT CTTTCTGGAG TGGAAGACCA CGTGAAAAAC 
TTTAGGCCAC AGTGTCTTGT TATGACAGGT GCTCCAAACT CACGTCCAGC TTTACTTCAT 
CTTGTTCATG ATTTCACAAA AAATGTTGGT TTGATGATCT GTGGCCATGT ACATATGGGT 
CCTCGAAGAC AAGCCATGAA AGAGATGTCC ATCGATCAAG CCAAATATCA GCGATGGCTT 
ATTAAGAACA AAATGAAGGC ATTTTATGCT CCAGTACATG CAGATGACTT GAGAGAAGGT 
GCACAGTATT TGATGCAGGC TGCTGGTCTT GGTCGTATGA AGCCAAACAC ACTTGTCCTT 
GGATTTAAGA AAGATTGGTT GCAAGCAGAT ATGAGGGATG TGGATATGTA TATAAACTTA 
TTTCATGATG CTTTTGACAT ACAATATGGA GTAGTGGTTA TTCGCCTAAA AGAAGGTCTG 
GATATATCTC ATCTTCAAGG ACAAGAAGAA TTATTGTCAT CACAAGAGAA ATCTCCTGGC 
ACCAAGGATG TGGTAGTAAG TGTGGAATAT AGTAAAAAGT CCGATTTAGA TACTTCCAAA 
CCACTCAGTG AAAAACCAAT TACACACAAA GTTGAGGAAG AGGATGGCAA GACTGCAACT 
CAACCACTGT TGAAAAAAGA ATCCAAAGGC CCTATTGTGC CTTTAAATGT AGCTGACCAA 
AAGCTTCTTG AAGCTAGTAC ACAGTTTCAG AAAAAACAAG GAAAGAATAC TATTGATGTC 
TGGTGGCTTT TTGATGATGG AGGTTTGACC TTATTGATAC CTTACCTTCT GACGACCAAG 
AAAAAATGGA AAGACTGTAA GATCAGAGTA TTCATTGGTG GAAAGATAAA CAGAATAGAC 
CATGACCGGA GAGCGATGGC TACTTTGCTT AGCAAGTTCC GGATAGACTT TTCTGATATC 
ATGGTTCTAG GAGATATCAA TACCAAACCA AAGAAAGAAA ATATTATAGC TTTTGAGGAA 
ATCATTGAGC CATACAGACT TCATGAAGAT GATAAAGAGC AAGATATTGC AGATAAAATG 
AAAGAAGATG AACCATGGCG AATAACAGAT AATGAGCTTG AACTTTATAA GACCAAGACA 
TACCGGCAGA TCAGGTTAAA TGAGTTATTA AAGGAACATT CAAGCACAGC TAATATTATT 
GTCATGAGTC TCCCAGTTGC ACGAAAAGGT GCTGTGTCTA GTGCTCTCTA CATGGCATGG 
TTAGAAGCTC TATCTAAGGA CCTACCACCA ATCCTCCTAG TTCGTGGGAA TCATCAGAGT 
GTCCTTACCT TCTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A2 ( NM_001256461.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243390.1 Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant 2, mRNA.

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CloneID	OHu28209
Clone ID Related Accession (Same CDS sequence)	NM_001256461.2 , NM_001256461.1
Accession Version	NM_001256461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011332.8, AY280459.1, BC144221.1, AC008577.7 and AI493213.1. On May 31, 2019 this sequence version replaced NM_001256461.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC146839.1, SRR1803617.119671.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCGC GGCCCACGGC GCCCTCCTCC GGCGCCCCGG GACTGGCCGG GGTCGGGGAG 
ACGCCGTCAG CCGCTGCGCT GGCCGCAGCC AGGGTGGAAC TGCCCGGCAC GGCTGTGCCC 
TCGGTGCCGG AGGATGCTGC GCCCGCGAGC CGGGACGGCG GCGGGGTCCG CGATGAGGGC 
CCCGCGGCGG CCGGGGACGG GCTGGGCAGA CCCTTGGGGC CCACCCCGAG CCAGAGCCGT 
TTCCAGGTGG ACCTGGTTTC CGAGAACGCC GGGCGGGCCG CTGCTGCGGC GGCGGCGGCG 
GCGGCGGCAG CGGCGGCGGC TGGTGCTGGG GCGGGGGCCA AGCAGACCCC CGCGGACGGG 
GAAGCCAGCG GCGAGAGCGA GCCGGCTAAA GGCAGCGAGG AAGCCAAGGG CCGCTTCCGC 
GTGAACTTCG TGGACCCAGC TGCCTCCTCG TCGGCTGAAG ACAGCCTGTC AGATGCTGCC 
GGGGTCGGAG TCGACGGGCC CAACGTGAGC TTCCAGAACG GCGGGGACAC GGTGCTGAGC 
GAGGGCAGCA GCCTGCACTC CGGCGGCGGC GGCGGCAGTG GGCACCACCA GCACTACTAT 
TATGATACCC ACACCAACAC CTACTACCTG CGCACCTTCG GCCACAACAC CATGGACGCT 
GTGCCCAGGA TCGATCACTA CCGGCACACA GCCGCGCAGC TGGGCGAGAA GCTGCTCCGG 
CCTAGCCTGG CGGAGCTCCA CGACGAGCTG GAAAAGGAAC CTTTTGAGGA TGGCTTTGCA 
AATGGGGAAG AAAGTACTCC AACCAGAGAT GCTGTGGTCA CGTATACTGC AGAAAGTAAA 
GGAGTCGTGA AGTTTGGCTG GATCAAGGGT GTATTAGTAC GTTGTATGTT AAACATTTGG 
GGTGTGATGC TTTTCATTAG ATTGTCATGG ATTGTGGGTC AAGCTGGAAT AGGTCTATCA 
GTCCTTGTAA TAATGATGGC CACTGTTGTG ACAACTATCA CAGGATTGTC TACTTCAGCA 
ATAGCAACTA ATGGATTTGT AAGAGGAGGA GGAGCATATT ATTTAATATC TAGAAGTCTA 
GGGCCAGAAT TTGGTGGTGC AATTGGTCTA ATCTTCGCCT TTGCCAACGC TGTTGCAGTT 
GCTATGTATG TGGTTGGATT TGCAGAAACC GTGGTGGAGT TGCTTAAGGA ACATTCCATA 
CTTATGATAG ATGAAATCAA TGATATCCGA ATTATTGGAG CCATTACAGT CGTGATTCTT 
TTAGGTATCT CAGTAGCTGG AATGGAGTGG GAAGCAAAAG CTCAGATTGT TCTTTTGGTG 
ATCCTACTTC TTGCTATTGG TGATTTCGTC ATAGGAACAT TTATCCCACT GGAGAGCAAG 
AAGCCAAAAG GGTTTTTTGG TTATAAATCT GAAATATTTA ATGAGAACTT TGGGCCCGAT 
TTTCGAGAGG AAGAGACTTT CTTTTCTGTA TTTGCCATCT TTTTTCCTGC TGCAACTGGT 
ATTCTGGCTG GAGCAAATAT CTCAGGTGAT CTTGCAGATC CTCAGTCAGC CATACCCAAA 
GGAACACTCC TAGCCATTTT AATTACTACA TTGGTTTACG TAGGAATTGC AGTATCTGTA 
GGTTCTTGTG TTGTTCGAGA TGCCACTGGA AACGTTAATG ACACTATCGT AACAGAGCTA 
ACAAACTGTA CTTCTGCAGC CTGCAAATTA AACTTTGATT TTTCATCTTG TGAAAGCAGT 
CCTTGTTCCT ATGGCCTAAT GAACAACTTC CAGGTAATGA GTATGGTGTC AGGATTTACA 
CCACTAATTT CTGCAGGTAT ATTTTCAGCC ACTCTTTCTT CAGCATTAGC ATCCCTAGTG 
AGTGCTCCCA AAATATTTCA GGCTCTATGT AAGGACAACA TCTACCCAGC TTTCCAGATG 
TTTGCTAAAG GTTATGGGAA AAATAATGAA CCTCTTCGTG GCTACATCTT AACATTCTTA 
ATTGCACTTG GATTCATCTT AATTGCTGAA CTGAATGTTA TTGCACCAAT TATCTCAAAC 
TTCTTCCTTG CATCATATGC ATTGATCAAT TTTTCAGTAT TCCATGCATC ACTTGCAAAA 
TCTCCAGGAT GGCGTCCTGC ATTCAAATAC TACAACATGT GGATATCACT TCTTGGAGCA 
ATTCTTTGTT GCATAGTAAT GTTCGTCATT AACTGGTGGG CTGCATTGCT AACATATGTG 
ATAGTCCTTG GGCTGTATAT TTATGTTACC TACAAAAAAC CAGATGTGAA TTGGGGATCC 
TCTACACAAG CCCTGACTTA CCTGAATGCA CTGCAGCATT CAATTCGTCT TTCTGGAGTG 
GAAGACCACG TGAAAAACTT TAGGCCACAG TGTCTTGTTA TGACAGGTGC TCCAAACTCA 
CGTCCAGCTT TACTTCATCT TGTTCATGAT TTCACAAAAA ATGTTGGTTT GATGATCTGT 
GGCCATGTAC ATATGGGTCC TCGAAGACAA GCCATGAAAG AGATGTCCAT CGATCAAGCC 
AAATATCAGC GATGGCTTAT TAAGAACAAA ATGAAGGCAT TTTATGCTCC AGTACATGCA 
GATGACTTGA GAGAAGGTGC ACAGTATTTG ATGCAGGCTG CTGGTCTTGG TCGTATGAAG 
CCAAACACAC TTGTCCTTGG ATTTAAGAAA GATTGGTTGC AAGCAGATAT GAGGGATGTG 
GATATGTATA TAAACTTATT TCATGATGCT TTTGACATAC AATATGGAGT AGTGGTTATT 
CGCCTAAAAG AAGGTCTGGA TATATCTCAT CTTCAAGGAC AAGAAGAATT ATTGTCATCA 
CAAGAGAAAT CTCCTGGCAC CAAGGATGTG GTAGTAAGTG TGGAATATAG TAAAAAGTCC 
GATTTAGATA CTTCCAAACC ACTCAGTGAA AAACCAATTA CACACAAAGA ATCCAAAGGC 
CCTATTGTGC CTTTAAATGT AGCTGACCAA AAGCTTCTTG AAGCTAGTAC ACAGTTTCAG 
AAAAAACAAG GAAAGAATAC TATTGATGTC TGGTGGCTTT TTGATGATGG AGGTTTGACC 
TTATTGATAC CTTACCTTCT GACGACCAAG AAAAAATGGA AAGACTGTAA GATCAGAGTA 
TTCATTGGTG GAAAGATAAA CAGAATAGAC CATGACCGGA GAGCGATGGC TACTTTGCTT 
AGCAAGTTCC GGATAGACTT TTCTGATATC ATGGTTCTAG GAGATATCAA TACCAAACCA 
AAGAAAGAAA ATATTATAGC TTTTGAGGAA ATCATTGAGC CATACAGACT TCATGAAGAT 
GATAAAGAGC AAGATATTGC AGATAAAATG AAAGAAGATG AACCATGGCG AATAACAGAT 
AATGAGCTTG AACTTTATAA GACCAAGACA TACCGGCAGA TCAGGTTAAA TGAGTTATTA 
AAGGAACATT CAAGCACAGC TAATATTATT GTCATGAGTC TCCCAGTTGC ACGAAAAGGT 
GCTGTGTCTA GTGCTCTCTA CATGGCATGG TTAGAAGCTC TATCTAAGGA CCTACCACCA 
ATCCTCCTAG TTCGTGGGAA TCATCAGAGT GTCCTTACCT TCTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243390.1
CDS	190..3780
Translation	MEPRPTAPSSGAPGLAGVGETPSAAALAAARVELPGTAVPSVPEDAAPASRDGGGVRDEGPAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAAAAAAAAAAAGAGAGAKQTPADGEASGESEPAKGSEEAKGRFRVNFVDPAASSSAEDSLSDAAGVGVDGPNVSFQNGGDTVLSEGSSLHSGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFIPLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	NM_001256461.2
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256461.2

ORF Insert Sequence:

ATGGAGCCGC GGCCCACGGC GCCCTCCTCC GGCGCCCCGG GACTGGCCGG GGTCGGGGAG 
ACGCCGTCAG CCGCTGCGCT GGCCGCAGCC AGGGTGGAAC TGCCCGGCAC GGCTGTGCCC 
TCGGTGCCGG AGGATGCTGC GCCCGCGAGC CGGGACGGCG GCGGGGTCCG CGATGAGGGC 
CCCGCGGCGG CCGGGGACGG GCTGGGCAGA CCCTTGGGGC CCACCCCGAG CCAGAGCCGT 
TTCCAGGTGG ACCTGGTTTC CGAGAACGCC GGGCGGGCCG CTGCTGCGGC GGCGGCGGCG 
GCGGCGGCAG CGGCGGCGGC TGGTGCTGGG GCGGGGGCCA AGCAGACCCC CGCGGACGGG 
GAAGCCAGCG GCGAGAGCGA GCCGGCTAAA GGCAGCGAGG AAGCCAAGGG CCGCTTCCGC 
GTGAACTTCG TGGACCCAGC TGCCTCCTCG TCGGCTGAAG ACAGCCTGTC AGATGCTGCC 
GGGGTCGGAG TCGACGGGCC CAACGTGAGC TTCCAGAACG GCGGGGACAC GGTGCTGAGC 
GAGGGCAGCA GCCTGCACTC CGGCGGCGGC GGCGGCAGTG GGCACCACCA GCACTACTAT 
TATGATACCC ACACCAACAC CTACTACCTG CGCACCTTCG GCCACAACAC CATGGACGCT 
GTGCCCAGGA TCGATCACTA CCGGCACACA GCCGCGCAGC TGGGCGAGAA GCTGCTCCGG 
CCTAGCCTGG CGGAGCTCCA CGACGAGCTG GAAAAGGAAC CTTTTGAGGA TGGCTTTGCA 
AATGGGGAAG AAAGTACTCC AACCAGAGAT GCTGTGGTCA CGTATACTGC AGAAAGTAAA 
GGAGTCGTGA AGTTTGGCTG GATCAAGGGT GTATTAGTAC GTTGTATGTT AAACATTTGG 
GGTGTGATGC TTTTCATTAG ATTGTCATGG ATTGTGGGTC AAGCTGGAAT AGGTCTATCA 
GTCCTTGTAA TAATGATGGC CACTGTTGTG ACAACTATCA CAGGATTGTC TACTTCAGCA 
ATAGCAACTA ATGGATTTGT AAGAGGAGGA GGAGCATATT ATTTAATATC TAGAAGTCTA 
GGGCCAGAAT TTGGTGGTGC AATTGGTCTA ATCTTCGCCT TTGCCAACGC TGTTGCAGTT 
GCTATGTATG TGGTTGGATT TGCAGAAACC GTGGTGGAGT TGCTTAAGGA ACATTCCATA 
CTTATGATAG ATGAAATCAA TGATATCCGA ATTATTGGAG CCATTACAGT CGTGATTCTT 
TTAGGTATCT CAGTAGCTGG AATGGAGTGG GAAGCAAAAG CTCAGATTGT TCTTTTGGTG 
ATCCTACTTC TTGCTATTGG TGATTTCGTC ATAGGAACAT TTATCCCACT GGAGAGCAAG 
AAGCCAAAAG GGTTTTTTGG TTATAAATCT GAAATATTTA ATGAGAACTT TGGGCCCGAT 
TTTCGAGAGG AAGAGACTTT CTTTTCTGTA TTTGCCATCT TTTTTCCTGC TGCAACTGGT 
ATTCTGGCTG GAGCAAATAT CTCAGGTGAT CTTGCAGATC CTCAGTCAGC CATACCCAAA 
GGAACACTCC TAGCCATTTT AATTACTACA TTGGTTTACG TAGGAATTGC AGTATCTGTA 
GGTTCTTGTG TTGTTCGAGA TGCCACTGGA AACGTTAATG ACACTATCGT AACAGAGCTA 
ACAAACTGTA CTTCTGCAGC CTGCAAATTA AACTTTGATT TTTCATCTTG TGAAAGCAGT 
CCTTGTTCCT ATGGCCTAAT GAACAACTTC CAGGTAATGA GTATGGTGTC AGGATTTACA 
CCACTAATTT CTGCAGGTAT ATTTTCAGCC ACTCTTTCTT CAGCATTAGC ATCCCTAGTG 
AGTGCTCCCA AAATATTTCA GGCTCTATGT AAGGACAACA TCTACCCAGC TTTCCAGATG 
TTTGCTAAAG GTTATGGGAA AAATAATGAA CCTCTTCGTG GCTACATCTT AACATTCTTA 
ATTGCACTTG GATTCATCTT AATTGCTGAA CTGAATGTTA TTGCACCAAT TATCTCAAAC 
TTCTTCCTTG CATCATATGC ATTGATCAAT TTTTCAGTAT TCCATGCATC ACTTGCAAAA 
TCTCCAGGAT GGCGTCCTGC ATTCAAATAC TACAACATGT GGATATCACT TCTTGGAGCA 
ATTCTTTGTT GCATAGTAAT GTTCGTCATT AACTGGTGGG CTGCATTGCT AACATATGTG 
ATAGTCCTTG GGCTGTATAT TTATGTTACC TACAAAAAAC CAGATGTGAA TTGGGGATCC 
TCTACACAAG CCCTGACTTA CCTGAATGCA CTGCAGCATT CAATTCGTCT TTCTGGAGTG 
GAAGACCACG TGAAAAACTT TAGGCCACAG TGTCTTGTTA TGACAGGTGC TCCAAACTCA 
CGTCCAGCTT TACTTCATCT TGTTCATGAT TTCACAAAAA ATGTTGGTTT GATGATCTGT 
GGCCATGTAC ATATGGGTCC TCGAAGACAA GCCATGAAAG AGATGTCCAT CGATCAAGCC 
AAATATCAGC GATGGCTTAT TAAGAACAAA ATGAAGGCAT TTTATGCTCC AGTACATGCA 
GATGACTTGA GAGAAGGTGC ACAGTATTTG ATGCAGGCTG CTGGTCTTGG TCGTATGAAG 
CCAAACACAC TTGTCCTTGG ATTTAAGAAA GATTGGTTGC AAGCAGATAT GAGGGATGTG 
GATATGTATA TAAACTTATT TCATGATGCT TTTGACATAC AATATGGAGT AGTGGTTATT 
CGCCTAAAAG AAGGTCTGGA TATATCTCAT CTTCAAGGAC AAGAAGAATT ATTGTCATCA 
CAAGAGAAAT CTCCTGGCAC CAAGGATGTG GTAGTAAGTG TGGAATATAG TAAAAAGTCC 
GATTTAGATA CTTCCAAACC ACTCAGTGAA AAACCAATTA CACACAAAGA ATCCAAAGGC 
CCTATTGTGC CTTTAAATGT AGCTGACCAA AAGCTTCTTG AAGCTAGTAC ACAGTTTCAG 
AAAAAACAAG GAAAGAATAC TATTGATGTC TGGTGGCTTT TTGATGATGG AGGTTTGACC 
TTATTGATAC CTTACCTTCT GACGACCAAG AAAAAATGGA AAGACTGTAA GATCAGAGTA 
TTCATTGGTG GAAAGATAAA CAGAATAGAC CATGACCGGA GAGCGATGGC TACTTTGCTT 
AGCAAGTTCC GGATAGACTT TTCTGATATC ATGGTTCTAG GAGATATCAA TACCAAACCA 
AAGAAAGAAA ATATTATAGC TTTTGAGGAA ATCATTGAGC CATACAGACT TCATGAAGAT 
GATAAAGAGC AAGATATTGC AGATAAAATG AAAGAAGATG AACCATGGCG AATAACAGAT 
AATGAGCTTG AACTTTATAA GACCAAGACA TACCGGCAGA TCAGGTTAAA TGAGTTATTA 
AAGGAACATT CAAGCACAGC TAATATTATT GTCATGAGTC TCCCAGTTGC ACGAAAAGGT 
GCTGTGTCTA GTGCTCTCTA CATGGCATGG TTAGAAGCTC TATCTAAGGA CCTACCACCA 
ATCCTCCTAG TTCGTGGGAA TCATCAGAGT GTCCTTACCT TCTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A2 ( NM_001256461.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243390.1 Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant 2, mRNA.

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CloneID	OHu28209
Clone ID Related Accession (Same CDS sequence)	NM_001256461.2 , NM_001256461.1
Accession Version	NM_001256461.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY280459.1, AC011332.8, BC144221.1, AC008577.7 and AI493213.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144221.1, SRR1803617.119671.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCGC GGCCCACGGC GCCCTCCTCC GGCGCCCCGG GACTGGCCGG GGTCGGGGAG 
ACGCCGTCAG CCGCTGCGCT GGCCGCAGCC AGGGTGGAAC TGCCCGGCAC GGCTGTGCCC 
TCGGTGCCGG AGGATGCTGC GCCCGCGAGC CGGGACGGCG GCGGGGTCCG CGATGAGGGC 
CCCGCGGCGG CCGGGGACGG GCTGGGCAGA CCCTTGGGGC CCACCCCGAG CCAGAGCCGT 
TTCCAGGTGG ACCTGGTTTC CGAGAACGCC GGGCGGGCCG CTGCTGCGGC GGCGGCGGCG 
GCGGCGGCAG CGGCGGCGGC TGGTGCTGGG GCGGGGGCCA AGCAGACCCC CGCGGACGGG 
GAAGCCAGCG GCGAGAGCGA GCCGGCTAAA GGCAGCGAGG AAGCCAAGGG CCGCTTCCGC 
GTGAACTTCG TGGACCCAGC TGCCTCCTCG TCGGCTGAAG ACAGCCTGTC AGATGCTGCC 
GGGGTCGGAG TCGACGGGCC CAACGTGAGC TTCCAGAACG GCGGGGACAC GGTGCTGAGC 
GAGGGCAGCA GCCTGCACTC CGGCGGCGGC GGCGGCAGTG GGCACCACCA GCACTACTAT 
TATGATACCC ACACCAACAC CTACTACCTG CGCACCTTCG GCCACAACAC CATGGACGCT 
GTGCCCAGGA TCGATCACTA CCGGCACACA GCCGCGCAGC TGGGCGAGAA GCTGCTCCGG 
CCTAGCCTGG CGGAGCTCCA CGACGAGCTG GAAAAGGAAC CTTTTGAGGA TGGCTTTGCA 
AATGGGGAAG AAAGTACTCC AACCAGAGAT GCTGTGGTCA CGTATACTGC AGAAAGTAAA 
GGAGTCGTGA AGTTTGGCTG GATCAAGGGT GTATTAGTAC GTTGTATGTT AAACATTTGG 
GGTGTGATGC TTTTCATTAG ATTGTCATGG ATTGTGGGTC AAGCTGGAAT AGGTCTATCA 
GTCCTTGTAA TAATGATGGC CACTGTTGTG ACAACTATCA CAGGATTGTC TACTTCAGCA 
ATAGCAACTA ATGGATTTGT AAGAGGAGGA GGAGCATATT ATTTAATATC TAGAAGTCTA 
GGGCCAGAAT TTGGTGGTGC AATTGGTCTA ATCTTCGCCT TTGCCAACGC TGTTGCAGTT 
GCTATGTATG TGGTTGGATT TGCAGAAACC GTGGTGGAGT TGCTTAAGGA ACATTCCATA 
CTTATGATAG ATGAAATCAA TGATATCCGA ATTATTGGAG CCATTACAGT CGTGATTCTT 
TTAGGTATCT CAGTAGCTGG AATGGAGTGG GAAGCAAAAG CTCAGATTGT TCTTTTGGTG 
ATCCTACTTC TTGCTATTGG TGATTTCGTC ATAGGAACAT TTATCCCACT GGAGAGCAAG 
AAGCCAAAAG GGTTTTTTGG TTATAAATCT GAAATATTTA ATGAGAACTT TGGGCCCGAT 
TTTCGAGAGG AAGAGACTTT CTTTTCTGTA TTTGCCATCT TTTTTCCTGC TGCAACTGGT 
ATTCTGGCTG GAGCAAATAT CTCAGGTGAT CTTGCAGATC CTCAGTCAGC CATACCCAAA 
GGAACACTCC TAGCCATTTT AATTACTACA TTGGTTTACG TAGGAATTGC AGTATCTGTA 
GGTTCTTGTG TTGTTCGAGA TGCCACTGGA AACGTTAATG ACACTATCGT AACAGAGCTA 
ACAAACTGTA CTTCTGCAGC CTGCAAATTA AACTTTGATT TTTCATCTTG TGAAAGCAGT 
CCTTGTTCCT ATGGCCTAAT GAACAACTTC CAGGTAATGA GTATGGTGTC AGGATTTACA 
CCACTAATTT CTGCAGGTAT ATTTTCAGCC ACTCTTTCTT CAGCATTAGC ATCCCTAGTG 
AGTGCTCCCA AAATATTTCA GGCTCTATGT AAGGACAACA TCTACCCAGC TTTCCAGATG 
TTTGCTAAAG GTTATGGGAA AAATAATGAA CCTCTTCGTG GCTACATCTT AACATTCTTA 
ATTGCACTTG GATTCATCTT AATTGCTGAA CTGAATGTTA TTGCACCAAT TATCTCAAAC 
TTCTTCCTTG CATCATATGC ATTGATCAAT TTTTCAGTAT TCCATGCATC ACTTGCAAAA 
TCTCCAGGAT GGCGTCCTGC ATTCAAATAC TACAACATGT GGATATCACT TCTTGGAGCA 
ATTCTTTGTT GCATAGTAAT GTTCGTCATT AACTGGTGGG CTGCATTGCT AACATATGTG 
ATAGTCCTTG GGCTGTATAT TTATGTTACC TACAAAAAAC CAGATGTGAA TTGGGGATCC 
TCTACACAAG CCCTGACTTA CCTGAATGCA CTGCAGCATT CAATTCGTCT TTCTGGAGTG 
GAAGACCACG TGAAAAACTT TAGGCCACAG TGTCTTGTTA TGACAGGTGC TCCAAACTCA 
CGTCCAGCTT TACTTCATCT TGTTCATGAT TTCACAAAAA ATGTTGGTTT GATGATCTGT 
GGCCATGTAC ATATGGGTCC TCGAAGACAA GCCATGAAAG AGATGTCCAT CGATCAAGCC 
AAATATCAGC GATGGCTTAT TAAGAACAAA ATGAAGGCAT TTTATGCTCC AGTACATGCA 
GATGACTTGA GAGAAGGTGC ACAGTATTTG ATGCAGGCTG CTGGTCTTGG TCGTATGAAG 
CCAAACACAC TTGTCCTTGG ATTTAAGAAA GATTGGTTGC AAGCAGATAT GAGGGATGTG 
GATATGTATA TAAACTTATT TCATGATGCT TTTGACATAC AATATGGAGT AGTGGTTATT 
CGCCTAAAAG AAGGTCTGGA TATATCTCAT CTTCAAGGAC AAGAAGAATT ATTGTCATCA 
CAAGAGAAAT CTCCTGGCAC CAAGGATGTG GTAGTAAGTG TGGAATATAG TAAAAAGTCC 
GATTTAGATA CTTCCAAACC ACTCAGTGAA AAACCAATTA CACACAAAGA ATCCAAAGGC 
CCTATTGTGC CTTTAAATGT AGCTGACCAA AAGCTTCTTG AAGCTAGTAC ACAGTTTCAG 
AAAAAACAAG GAAAGAATAC TATTGATGTC TGGTGGCTTT TTGATGATGG AGGTTTGACC 
TTATTGATAC CTTACCTTCT GACGACCAAG AAAAAATGGA AAGACTGTAA GATCAGAGTA 
TTCATTGGTG GAAAGATAAA CAGAATAGAC CATGACCGGA GAGCGATGGC TACTTTGCTT 
AGCAAGTTCC GGATAGACTT TTCTGATATC ATGGTTCTAG GAGATATCAA TACCAAACCA 
AAGAAAGAAA ATATTATAGC TTTTGAGGAA ATCATTGAGC CATACAGACT TCATGAAGAT 
GATAAAGAGC AAGATATTGC AGATAAAATG AAAGAAGATG AACCATGGCG AATAACAGAT 
AATGAGCTTG AACTTTATAA GACCAAGACA TACCGGCAGA TCAGGTTAAA TGAGTTATTA 
AAGGAACATT CAAGCACAGC TAATATTATT GTCATGAGTC TCCCAGTTGC ACGAAAAGGT 
GCTGTGTCTA GTGCTCTCTA CATGGCATGG TTAGAAGCTC TATCTAAGGA CCTACCACCA 
ATCCTCCTAG TTCGTGGGAA TCATCAGAGT GTCCTTACCT TCTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243390.1
CDS	165..3755
Translation	MEPRPTAPSSGAPGLAGVGETPSAAALAAARVELPGTAVPSVPEDAAPASRDGGGVRDEGPAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAAAAAAAAAAAGAGAGAKQTPADGEASGESEPAKGSEEAKGRFRVNFVDPAASSSAEDSLSDAAGVGVDGPNVSFQNGGDTVLSEGSSLHSGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFIPLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	NM_001256461.1
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256461.1

ORF Insert Sequence:

ATGGAGCCGC GGCCCACGGC GCCCTCCTCC GGCGCCCCGG GACTGGCCGG GGTCGGGGAG 
ACGCCGTCAG CCGCTGCGCT GGCCGCAGCC AGGGTGGAAC TGCCCGGCAC GGCTGTGCCC 
TCGGTGCCGG AGGATGCTGC GCCCGCGAGC CGGGACGGCG GCGGGGTCCG CGATGAGGGC 
CCCGCGGCGG CCGGGGACGG GCTGGGCAGA CCCTTGGGGC CCACCCCGAG CCAGAGCCGT 
TTCCAGGTGG ACCTGGTTTC CGAGAACGCC GGGCGGGCCG CTGCTGCGGC GGCGGCGGCG 
GCGGCGGCAG CGGCGGCGGC TGGTGCTGGG GCGGGGGCCA AGCAGACCCC CGCGGACGGG 
GAAGCCAGCG GCGAGAGCGA GCCGGCTAAA GGCAGCGAGG AAGCCAAGGG CCGCTTCCGC 
GTGAACTTCG TGGACCCAGC TGCCTCCTCG TCGGCTGAAG ACAGCCTGTC AGATGCTGCC 
GGGGTCGGAG TCGACGGGCC CAACGTGAGC TTCCAGAACG GCGGGGACAC GGTGCTGAGC 
GAGGGCAGCA GCCTGCACTC CGGCGGCGGC GGCGGCAGTG GGCACCACCA GCACTACTAT 
TATGATACCC ACACCAACAC CTACTACCTG CGCACCTTCG GCCACAACAC CATGGACGCT 
GTGCCCAGGA TCGATCACTA CCGGCACACA GCCGCGCAGC TGGGCGAGAA GCTGCTCCGG 
CCTAGCCTGG CGGAGCTCCA CGACGAGCTG GAAAAGGAAC CTTTTGAGGA TGGCTTTGCA 
AATGGGGAAG AAAGTACTCC AACCAGAGAT GCTGTGGTCA CGTATACTGC AGAAAGTAAA 
GGAGTCGTGA AGTTTGGCTG GATCAAGGGT GTATTAGTAC GTTGTATGTT AAACATTTGG 
GGTGTGATGC TTTTCATTAG ATTGTCATGG ATTGTGGGTC AAGCTGGAAT AGGTCTATCA 
GTCCTTGTAA TAATGATGGC CACTGTTGTG ACAACTATCA CAGGATTGTC TACTTCAGCA 
ATAGCAACTA ATGGATTTGT AAGAGGAGGA GGAGCATATT ATTTAATATC TAGAAGTCTA 
GGGCCAGAAT TTGGTGGTGC AATTGGTCTA ATCTTCGCCT TTGCCAACGC TGTTGCAGTT 
GCTATGTATG TGGTTGGATT TGCAGAAACC GTGGTGGAGT TGCTTAAGGA ACATTCCATA 
CTTATGATAG ATGAAATCAA TGATATCCGA ATTATTGGAG CCATTACAGT CGTGATTCTT 
TTAGGTATCT CAGTAGCTGG AATGGAGTGG GAAGCAAAAG CTCAGATTGT TCTTTTGGTG 
ATCCTACTTC TTGCTATTGG TGATTTCGTC ATAGGAACAT TTATCCCACT GGAGAGCAAG 
AAGCCAAAAG GGTTTTTTGG TTATAAATCT GAAATATTTA ATGAGAACTT TGGGCCCGAT 
TTTCGAGAGG AAGAGACTTT CTTTTCTGTA TTTGCCATCT TTTTTCCTGC TGCAACTGGT 
ATTCTGGCTG GAGCAAATAT CTCAGGTGAT CTTGCAGATC CTCAGTCAGC CATACCCAAA 
GGAACACTCC TAGCCATTTT AATTACTACA TTGGTTTACG TAGGAATTGC AGTATCTGTA 
GGTTCTTGTG TTGTTCGAGA TGCCACTGGA AACGTTAATG ACACTATCGT AACAGAGCTA 
ACAAACTGTA CTTCTGCAGC CTGCAAATTA AACTTTGATT TTTCATCTTG TGAAAGCAGT 
CCTTGTTCCT ATGGCCTAAT GAACAACTTC CAGGTAATGA GTATGGTGTC AGGATTTACA 
CCACTAATTT CTGCAGGTAT ATTTTCAGCC ACTCTTTCTT CAGCATTAGC ATCCCTAGTG 
AGTGCTCCCA AAATATTTCA GGCTCTATGT AAGGACAACA TCTACCCAGC TTTCCAGATG 
TTTGCTAAAG GTTATGGGAA AAATAATGAA CCTCTTCGTG GCTACATCTT AACATTCTTA 
ATTGCACTTG GATTCATCTT AATTGCTGAA CTGAATGTTA TTGCACCAAT TATCTCAAAC 
TTCTTCCTTG CATCATATGC ATTGATCAAT TTTTCAGTAT TCCATGCATC ACTTGCAAAA 
TCTCCAGGAT GGCGTCCTGC ATTCAAATAC TACAACATGT GGATATCACT TCTTGGAGCA 
ATTCTTTGTT GCATAGTAAT GTTCGTCATT AACTGGTGGG CTGCATTGCT AACATATGTG 
ATAGTCCTTG GGCTGTATAT TTATGTTACC TACAAAAAAC CAGATGTGAA TTGGGGATCC 
TCTACACAAG CCCTGACTTA CCTGAATGCA CTGCAGCATT CAATTCGTCT TTCTGGAGTG 
GAAGACCACG TGAAAAACTT TAGGCCACAG TGTCTTGTTA TGACAGGTGC TCCAAACTCA 
CGTCCAGCTT TACTTCATCT TGTTCATGAT TTCACAAAAA ATGTTGGTTT GATGATCTGT 
GGCCATGTAC ATATGGGTCC TCGAAGACAA GCCATGAAAG AGATGTCCAT CGATCAAGCC 
AAATATCAGC GATGGCTTAT TAAGAACAAA ATGAAGGCAT TTTATGCTCC AGTACATGCA 
GATGACTTGA GAGAAGGTGC ACAGTATTTG ATGCAGGCTG CTGGTCTTGG TCGTATGAAG 
CCAAACACAC TTGTCCTTGG ATTTAAGAAA GATTGGTTGC AAGCAGATAT GAGGGATGTG 
GATATGTATA TAAACTTATT TCATGATGCT TTTGACATAC AATATGGAGT AGTGGTTATT 
CGCCTAAAAG AAGGTCTGGA TATATCTCAT CTTCAAGGAC AAGAAGAATT ATTGTCATCA 
CAAGAGAAAT CTCCTGGCAC CAAGGATGTG GTAGTAAGTG TGGAATATAG TAAAAAGTCC 
GATTTAGATA CTTCCAAACC ACTCAGTGAA AAACCAATTA CACACAAAGA ATCCAAAGGC 
CCTATTGTGC CTTTAAATGT AGCTGACCAA AAGCTTCTTG AAGCTAGTAC ACAGTTTCAG 
AAAAAACAAG GAAAGAATAC TATTGATGTC TGGTGGCTTT TTGATGATGG AGGTTTGACC 
TTATTGATAC CTTACCTTCT GACGACCAAG AAAAAATGGA AAGACTGTAA GATCAGAGTA 
TTCATTGGTG GAAAGATAAA CAGAATAGAC CATGACCGGA GAGCGATGGC TACTTTGCTT 
AGCAAGTTCC GGATAGACTT TTCTGATATC ATGGTTCTAG GAGATATCAA TACCAAACCA 
AAGAAAGAAA ATATTATAGC TTTTGAGGAA ATCATTGAGC CATACAGACT TCATGAAGAT 
GATAAAGAGC AAGATATTGC AGATAAAATG AAAGAAGATG AACCATGGCG AATAACAGAT 
AATGAGCTTG AACTTTATAA GACCAAGACA TACCGGCAGA TCAGGTTAAA TGAGTTATTA 
AAGGAACATT CAAGCACAGC TAATATTATT GTCATGAGTC TCCCAGTTGC ACGAAAAGGT 
GCTGTGTCTA GTGCTCTCTA CATGGCATGG TTAGAAGCTC TATCTAAGGA CCTACCACCA 
ATCCTCCTAG TTCGTGGGAA TCATCAGAGT GTCCTTACCT TCTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A2 ( XM_011543588.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541890.1 Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant X2, mRNA.

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CloneID	OHu60598
Clone ID Related Accession (Same CDS sequence)	XM_011543588.2
Accession Version	XM_011543588.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543588.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC GGCCCACGGC GCCCTCCTCC GGCGCCCCGG GACTGGCCGG GGTCGGGGAG 
ACGCCGTCAG CCGCTGCGCT GGCCGCAGCC AGGGTGGAAC TGCCCGGCAC GGCTGTGCCC 
TCGGTGCCGG AGGATGCTGC GCCCGCGAGC CGGGACGGCG GCGGGGTCCG CGATGAGGGC 
CCCGCGGCGG CCGGGGACGG GCTGGGCAGA CCCTTGGGGC CCACCCCGAG CCAGAGCCGT 
TTCCAGGTGG ACCTGGTTTC CGAGAACGCC GGGCGGGCCG CTGCTGCGGC GGCGGCGGCG 
GCGGCGGCAG CGGCGGCGGC TGGTGCTGGG GCGGGGGCCA AGCAGACCCC CGCGGACGGG 
GAAGCCAGCG GCGAGAGCGA GCCGGCTAAA GGCAGCGAGG AAGCCAAGGG CCGCTTCCGC 
GTGAACTTCG TGGACCCAGC TGCCTCCTCG TCGGCTGAAG ACAGCCTGTC AGATGCTGCC 
GGGGTCGGAG TCGACGGGCC CAACGTGAGC TTCCAGAACG GCGGGGACAC GGTGCTGAGC 
GAGGGCAGCA GCCTGCACTC CGGCGGCGGC GGCGGCAGTG GGCACCACCA GCACTACTAT 
TATGATACCC ACACCAACAC CTACTACCTG CGCACCTTCG GCCACAACAC CATGGACGCT 
GTGCCCAGGA TCGATCACTA CCGGCACACA GCCGCGCAGC TGGGCGAGAA GCTGCTCCGG 
CCTAGCCTGG CGGAGCTCCA CGACGAGCTG GAAAAGGAAC CTTTTGAGGA TGGCTTTGCA 
AATGGGGAAG AAAGTACTCC AACCAGAGAT GCTGTGGTCA CGTATACTGC AGAAAGTAAA 
GGAGTCGTGA AGTTTGGCTG GATCAAGGGT GTATTAGTAC GTTGTATGTT AAACATTTGG 
GGTGTGATGC TTTTCATTAG ATTGTCATGG ATTGTGGGTC AAGCTGGAAT AGGTCTATCA 
GTCCTTGTAA TAATGATGGC CACTGTTGTG ACAACTATCA CAGGATTGTC TACTTCAGCA 
ATAGCAACTA ATGGATTTGT AAGAGGAGGA GGAGCATATT ATTTAATATC TAGAAGTCTA 
GGGCCAGAAT TTGGTGGTGC AATTGGTCTA ATCTTCGCCT TTGCCAACGC TGTTGCAGTT 
GCTATGTATG TGGTTGGATT TGCAGAAACC GTGGTGGAGT TGCTTAAGGA ACATTCCATA 
CTTATGATAG ATGAAATCAA TGATATCCGA ATTATTGGAG CCATTACAGT CGTGATTCTT 
TTAGGTATCT CAGTAGCTGG AATGGAGTGG GAAGCAAAAG CTCAGATTGT TCTTTTGGTG 
ATCCTACTTC TTGCTATTGG TGATTTCGTC ATAGGAACAT TTATCCCACT GGAGAGCAAG 
AAGCCAAAAG GGTTTTTTGG TTATAAATCT GAAATATTTA ATGAGAACTT TGGGCCCGAT 
TTTCGAGAGG AAGAGACTTT CTTTTCTGTA TTTGCCATCT TTTTTCCTGC TGCAACTGGT 
ATTCTGGCTG GAGCAAATAT CTCAGGTGAT CTTGCAGATC CTCAGTCAGC CATACCCAAA 
GGAACACTCC TAGCCATTTT AATTACTACA TTGGTTTACG TAGGAATTGC AGTATCTGTA 
GGTTCTTGTG TTGTTCGAGA TGCCACTGGA AACGTTAATG ACACTATCGT AACAGAGCTA 
ACAAACTGTA CTTCTGCAGC CTGCAAATTA AACTTTGATT TTTCATCTTG TGAAAGCAGT 
CCTTGTTCCT ATGGCCTAAT GAACAACTTC CAGTCAGCAT TTTGTAGTTT TCAGCATATA 
CGTCCTGTAT GTATTTTGTT AGATTTACGA GTAAGTATTT TTATTTTGCT CAATTTAAAC 
CCATACCAAT TAAGTTCAGT TTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541890.1
CDS	159..2045
Translation	MEPRPTAPSSGAPGLAGVGETPSAAALAAARVELPGTAVPSVPEDAAPASRDGGGVRDEGPAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAAAAAAAAAAAGAGAGAKQTPADGEASGESEPAKGSEEAKGRFRVNFVDPAASSSAEDSLSDAAGVGVDGPNVSFQNGGDTVLSEGSSLHSGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFIPLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQSAFCSFQHIRPVCILLDLRVSIFILLNLNPYQLSSVF

Target ORF information:

RefSeq Version	XM_011543588.2
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 2 (SLC12A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543588.2

ORF Insert Sequence:

ATGGAGCCGC GGCCCACGGC GCCCTCCTCC GGCGCCCCGG GACTGGCCGG GGTCGGGGAG 
ACGCCGTCAG CCGCTGCGCT GGCCGCAGCC AGGGTGGAAC TGCCCGGCAC GGCTGTGCCC 
TCGGTGCCGG AGGATGCTGC GCCCGCGAGC CGGGACGGCG GCGGGGTCCG CGATGAGGGC 
CCCGCGGCGG CCGGGGACGG GCTGGGCAGA CCCTTGGGGC CCACCCCGAG CCAGAGCCGT 
TTCCAGGTGG ACCTGGTTTC CGAGAACGCC GGGCGGGCCG CTGCTGCGGC GGCGGCGGCG 
GCGGCGGCAG CGGCGGCGGC TGGTGCTGGG GCGGGGGCCA AGCAGACCCC CGCGGACGGG 
GAAGCCAGCG GCGAGAGCGA GCCGGCTAAA GGCAGCGAGG AAGCCAAGGG CCGCTTCCGC 
GTGAACTTCG TGGACCCAGC TGCCTCCTCG TCGGCTGAAG ACAGCCTGTC AGATGCTGCC 
GGGGTCGGAG TCGACGGGCC CAACGTGAGC TTCCAGAACG GCGGGGACAC GGTGCTGAGC 
GAGGGCAGCA GCCTGCACTC CGGCGGCGGC GGCGGCAGTG GGCACCACCA GCACTACTAT 
TATGATACCC ACACCAACAC CTACTACCTG CGCACCTTCG GCCACAACAC CATGGACGCT 
GTGCCCAGGA TCGATCACTA CCGGCACACA GCCGCGCAGC TGGGCGAGAA GCTGCTCCGG 
CCTAGCCTGG CGGAGCTCCA CGACGAGCTG GAAAAGGAAC CTTTTGAGGA TGGCTTTGCA 
AATGGGGAAG AAAGTACTCC AACCAGAGAT GCTGTGGTCA CGTATACTGC AGAAAGTAAA 
GGAGTCGTGA AGTTTGGCTG GATCAAGGGT GTATTAGTAC GTTGTATGTT AAACATTTGG 
GGTGTGATGC TTTTCATTAG ATTGTCATGG ATTGTGGGTC AAGCTGGAAT AGGTCTATCA 
GTCCTTGTAA TAATGATGGC CACTGTTGTG ACAACTATCA CAGGATTGTC TACTTCAGCA 
ATAGCAACTA ATGGATTTGT AAGAGGAGGA GGAGCATATT ATTTAATATC TAGAAGTCTA 
GGGCCAGAAT TTGGTGGTGC AATTGGTCTA ATCTTCGCCT TTGCCAACGC TGTTGCAGTT 
GCTATGTATG TGGTTGGATT TGCAGAAACC GTGGTGGAGT TGCTTAAGGA ACATTCCATA 
CTTATGATAG ATGAAATCAA TGATATCCGA ATTATTGGAG CCATTACAGT CGTGATTCTT 
TTAGGTATCT CAGTAGCTGG AATGGAGTGG GAAGCAAAAG CTCAGATTGT TCTTTTGGTG 
ATCCTACTTC TTGCTATTGG TGATTTCGTC ATAGGAACAT TTATCCCACT GGAGAGCAAG 
AAGCCAAAAG GGTTTTTTGG TTATAAATCT GAAATATTTA ATGAGAACTT TGGGCCCGAT 
TTTCGAGAGG AAGAGACTTT CTTTTCTGTA TTTGCCATCT TTTTTCCTGC TGCAACTGGT 
ATTCTGGCTG GAGCAAATAT CTCAGGTGAT CTTGCAGATC CTCAGTCAGC CATACCCAAA 
GGAACACTCC TAGCCATTTT AATTACTACA TTGGTTTACG TAGGAATTGC AGTATCTGTA 
GGTTCTTGTG TTGTTCGAGA TGCCACTGGA AACGTTAATG ACACTATCGT AACAGAGCTA 
ACAAACTGTA CTTCTGCAGC CTGCAAATTA AACTTTGATT TTTCATCTTG TGAAAGCAGT 
CCTTGTTCCT ATGGCCTAAT GAACAACTTC CAGTCAGCAT TTTGTAGTTT TCAGCATATA 
CGTCCTGTAT GTATTTTGTT AGATTTACGA GTAAGTATTT TTATTTTGCT CAATTTAAAC 
CCATACCAAT TAAGTTCAGT TTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NKCC1 Regulates Migration Ability of Glioblastoma Cells by Modulation of Actin Dynamics and Interacting with Cofilin.
EBioMedicine2194-103(2017 Jul)
Schiapparelli P,Guerrero-Cazares H,Maga?a-Maldonado R,Hamilla SM,Ganaha S,Goulin Lippi Fernandes E,Huang CH,Aranda-Espinoza H,Devreotes P,Quinones-Hinojosa A

A High-Throughput Screening Assay for NKCC1 Cotransporter Using Nonradioactive Rubidium Flux Technology.
Assay and drug development technologies15(4)167-177(2017 May/Jun)
Gill S,Gill R,Wen Y,Enderle T,Roth D,Liang D

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SLC12A2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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