SLC12A2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC12A2
Entrez Gene ID	286845
Full Name	solute carrier family 12 member 2
Synonyms	bsc2
General protein information	Preferred Names solute carrier family 12 member 2 Names solute carrier family 12 member 2 basolateral NaK(2Cl) cotransporter solute carrier family 12 (sodium/potassium/chloride transporters), member 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005209087.2	XP_005209144.1	solute carrier family 12 member 2 isoform X2
XM_005209087.3	XP_005209144.1	solute carrier family 12 member 2 isoform X2
XM_005209086.3	XP_005209143.1	solute carrier family 12 member 2 isoform X1
XM_005209086.2	XP_005209143.1	solute carrier family 12 member 2 isoform X1
NM_174782.3	NP_777207.1	solute carrier family 12 member 2

Pathways from BioSystems

Homologies

	SLC12A2	XP_001098624.1
Rattus norvegicus (Norway rat)	Slc12a2	NP_113986.1
Danio rerio (zebrafish)	slc12a2	NP_001002080.1
Drosophila melanogaster (fruit fly)	Ncc69	NP_648572.1
Homo sapiens (human)	SLC12A2	NP_001243390.1
Bos taurus (cattle)	SLC12A2	NP_777207.1
Gallus gallus (chicken)	SLC12A2	XP_004949435.1
Pan troglodytes (chimpanzee)	SLC12A2	XP_003310846.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001557	XP_321556.3
Xenopus tropicalis (tropical clawed frog)	slc12a2	XP_004910531.1
Canis lupus familiaris (dog)	SLC12A2	XP_538611.5
Mus musculus (house mouse)	Slc12a2	NP_033220.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Molecular characterization of the Na-K-Cl cotransporter of bovine aortic endothelial cells.
Yerby TR, Vibat CR, Sun D, Payne JA, O'Donnell ME
The American journal of physiology273(1 Pt 1)C188-97(1997 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SLC12A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa235787	XM_005209086.3 Latest version!	Bos taurus solute carrier family 12 member 2 (SLC12A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa235788	XM_005209087.3 Latest version!	Bos taurus solute carrier family 12 member 2 (SLC12A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa38982	NM_174782.3 Latest version!	Bos taurus solute carrier family 12 member 2 (SLC12A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa21259	XM_005209086.2 Latest version!	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa21260	XM_005209087.2 Latest version!	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC12A2 ( XM_005209086.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209143.1 Bos taurus solute carrier family 12 member 2 (SLC12A2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa235787
Clone ID Related Accession (Same CDS sequence)	XM_005209086.3
Accession Version	XM_005209086.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209086.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG GGCCCACGGC GCCCTCCTCC GGCGCCCCGA GGCTGGCCGG GGTTGGGGAG 
ACGCCTCCAG TCGCCCCGCT GGCCGCCGCC GGGATGGACC TGCCCGGCAC AGCCGTGCCC 
TCAGTGCCGG AGGATGCTGC GGCTGTGAGC CGTGGCGGCG GTGGGGTCCG CGGGGAGGGC 
GCCGCGGCGG CCGGCGACGG ACTGGGCAGA CCCCTGGGGC CCACCCCGAG TCAGAGCCGC 
TTCCAGGTGG ATCTGGTTTC CGAGAATGCC GGGCGGGCGG CGGCGGCGGC GGCTAGCGCT 
GGGGCGGGAG GCAAGGAGAC CCCCGCGGAT GGGAAAGCCA GCGTCGAAAG CGGGCAGGCT 
AAGGCCAGCG AGGAAGCCAA GGGCCGCTTC CGCGTGAACT TCGTGGACCC GGCTGCCTCT 
TCGTCGGCGG ACGAGAGCGT GTCGGATGCG GCAGGGATCG GAGGCGACGG GCCCAACGTG 
AGCTTCCAGA ACGGCGGGGA CACGGTGCTG AGCGAGGGGA GCAGCCTGCA CTCGGGCGGC 
GGCGGCGGCG GCAGTGGGCA CCACCAGCAC TACTACTATG ACACCCACAC CAACACCTAC 
TACCTGCGCA CCTTCGGCCA CAACACTATG GACGCCGTGC CCAGGATCGA CCACTATCGG 
CACACGGCAG CGCAGCTAGG CGAGAAGCTG CTCCGGCCCA GCCTGGCGGA ACTACACGAT 
GAGTTGGAAA AGGAACCTTT TGAGGATGGC TTTGCAAATG GAGAAGAAAG CACGCCAACC 
AGAGAGGCTG TGGTCACATA CACTGCGGAA AGTAAAGGAG TCGTGAAGTT TGGCTGGATT 
AAGGGTGTAC TAGTACGTTG TATGTTAAAC ATTTGGGGAG TGATGCTCTT TATTAGATTG 
TCATGGATTG TGGGTCAAGC TGGAATAGGT CTGTCAGTCA TTGTAATAGC AATGGCCACT 
GTCGTGACAA CTATCACAGG ATTGTCTACA TCAGCAATAG CTACTAATGG GTTTGTACGA 
GGAGGAGGAG CGTATTATTT AATATCTAGA AGTCTAGGGC CAGAATTTGG TGGTGCAATT 
GGCCTGATCT TCGCTTTTGC CAATGCCGTT GCAGTTGCTA TGTACGTGGT TGGATTTGCA 
GAAACTGTGG TGGAGTTGCT TAAGGAACAT TCCATACTTA TGATAGATGA AATCAATGAT 
ATCCGAATTA TTGGAGCCAT TACAGTGGTG ATCCTTTTGG GTATCTCAGT AGCCGGAATG 
GAGTGGGAGG CAAAAGCTCA GATTGTTCTT TTGGTGATTC TACTACTTGC TATAGCTGAT 
TTCGTCATAG GAACATTTAT CCCATTGGAG AGCAAGAAGC CCAAAGGTTT CTTTGGTTAT 
AAGTATGAAA TATTTAGTGA GAACTTTGGG CCAGATTTTC GAGAAGAAGA GACCTTTTTT 
TCTGTATTTG CCATCTTTTT TCCTGCTGCA ACTGGCATTC TGGCTGGAGC AAATATCTCA 
GGTGATCTTG CAGATCCTCA GTCAGCCATA CCCAAAGGAA CACTCTTAGC CATTTTAATC 
ACTACGCTGG TTTACATAGG AATTGCAGTA TCTGTAGGTT CGTGTGTTGT TCGGGACGCT 
ACTGGGAACG TTAATGACAC CATTGTAACA GAGCTGACAA ACTGCACTTC TGCAGCCTGC 
AAGTTAAACT TTGATTTTTC ATCCTGTGAA ACCAACCCTT GTTCCTATGG CCTAATGAAC 
AACTTCCAGG TAATGAGCAT GGTGTCAGGC TTTGCACCAT TAATTTCTGC AGGTATCTTC 
TCAGCCACTC TTTCTTCTGC CTTAGCATCT CTTGTGAGTG CACCCAAAAT ATTTCAGGCT 
CTATGTAAGG ACAACATCTA CCCAGCTTTC CAGATGTTTG CTAAAGGTTA TGGGAAAAAT 
AATGAACCTC TTCGTGGCTA CATCTTAACA TTTTTAATTG CACTTGGATT CATCTTAATT 
GCTGAATTGA ATGTTATTGC TCCAATTATC TCTAACTTCT TCCTTGCATC ATATGCACTG 
ATCAATTTTT CAGTATTTCA TGCATCACTT GCAAAATCTC CAGGGTGGCG TCCTGCATTC 
AAATACTACA ATATGTGGAT ATCACTTCTT GGAGCAATTC TCTGTTGCAT AGTGATGTTT 
GTCATTAACT GGTGGGCTGC ATTGCTAACA TATGTGATAG TTCTTGGACT TTACATTTAT 
GTTACCTACA AAAAACCAGA TGTGAACTGG GGATCCTCCA CACAAGCTCT GACCTACCTG 
AATGCACTGC AGCATTCGAT TCGTCTTTCT GGAGTGGAAG ACCACGTGAA AAACTTCAGG 
CCTCAGTGTC TTGTTATGAC AGGTGCGCCA AACTCACGCC CAGCTTTACT TCATCTTGTT 
CACGATTTCA CAAAAAATGT TGGCTTGATG ATCTGTGGTC ATGTGCATAT GGGCCCTCGA 
AGACAAGCTA TGAAAGAGAT GTCCATTGAT CAAGCCAAAT ATCAGCGGTG GCTTATTAAA 
AACAAAATGA AGGCTTTTTA TGCTCCAGTA CATGCAGATG ATTTGAGAGA GGGTGCACAA 
TATTTGATGC AGGCTGCTGG CCTTGGGCGT ATGAAACCAA ACACACTTGT CCTTGGATTT 
AAGAAAGACT GGTTGCAAGC AGATATGAGG GATGTAGATA TGTATATAAA CTTATTTCAT 
GATGCTTTTG ATATACAATA TGGAGTAGTA GTTATCCGCC TACAAGAAGG TCTGGATATA 
TCTCATCTTC AAGGACAAGA AGAATTACTG TCATCGCAAG AGAAATCTCC TGGTGTAAAG 
GATGTGGTAC TAAGTGTGGA GTATAGTAAA AAATCAGATT TAGATACTTC TAAACCATCC 
AGTGAGAAAT CCATTACACA TAAAGATGAG GAAGAGGATG GCAAGACTCC AACTCAACCA 
CTGTTGAAAA AAGAATCCAA AGGCCCTGCT GTGCCTTTAA ACGTAGCTGA CCAAAAGCTT 
CTTGAAGCTA GTACACAATT TCAGAAAAAA CAGGGAAAGA ATACTATTGA CGTCTGGTGG 
CTTTTTGATG ATGGAGGTTT GACTTTATTG ATACCTTATC TTCTGACTAC CAAGAAAAAG 
TGGAAAGATT GTAAGATCAG AGTATTCATT GGTGGAAAAA TAAATAGAAT AGACCATGAC 
CGAAGAGCGA TGGCTACTTT GCTTAGCAAG TTCCGGATAG ACTTCTCTGA TATCATGGTC 
TTAGGAGATA TTAATACTAA ACCAAAGAAA GAAAATATTG TAGCTTTTGA TGAAATGATT 
GAGCCATACA GACTTCATGA AGATGATAAA GAACAAGATA TTGCAGATAA AATGAAAGAA 
GATGAACCAT GGCGAATAAC AGATAATGAG CTTGAACTTT ATAAGACCAA GACATACCGG 
CAGATCAGGT TAAATGAATT ATTAAAGGAA CATTCAAACA CAGCTAATAT CATTGTCATG 
AGTCTCCCAG TTGCACGAAA AGGTGCTGTG TCTAGTGCTC TCTACATGGC ATGGTTAGAA 
GCTCTATCCA AGGACCTGCC ACCAATTCTC CTAGTCCGTG GGAATCATCA GAGTGTCCTT 
ACCTTCTATT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209143.1
CDS	480..4094
Translation	MEPGPTAPSSGAPRLAGVGETPPVAPLAAAGMDLPGTAVPSVPEDAAAVSRGGGGVRGEGAAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAASAGAGGKETPADGKASVESGQAKASEEAKGRFRVNFVDPAASSSADESVSDAAGIGGDGPNVSFQNGGDTVLSEGSSLHSGGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTREAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVIVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFIPLESKKPKGFFGYKYEIFSENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYIGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCETNPCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLQEGLDISHLQGQEELLSSQEKSPGVKDVVLSVEYSKKSDLDTSKPSSEKSITHKDEEEDGKTPTQPLLKKESKGPAVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIVAFDEMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSNTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	XM_005209086.3
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 member 2 (SLC12A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209086.3

ORF Insert Sequence:

ATGGAGCCGG GGCCCACGGC GCCCTCCTCC GGCGCCCCGA GGCTGGCCGG GGTTGGGGAG 
ACGCCTCCAG TCGCCCCGCT GGCCGCCGCC GGGATGGACC TGCCCGGCAC AGCCGTGCCC 
TCAGTGCCGG AGGATGCTGC GGCTGTGAGC CGTGGCGGCG GTGGGGTCCG CGGGGAGGGC 
GCCGCGGCGG CCGGCGACGG ACTGGGCAGA CCCCTGGGGC CCACCCCGAG TCAGAGCCGC 
TTCCAGGTGG ATCTGGTTTC CGAGAATGCC GGGCGGGCGG CGGCGGCGGC GGCTAGCGCT 
GGGGCGGGAG GCAAGGAGAC CCCCGCGGAT GGGAAAGCCA GCGTCGAAAG CGGGCAGGCT 
AAGGCCAGCG AGGAAGCCAA GGGCCGCTTC CGCGTGAACT TCGTGGACCC GGCTGCCTCT 
TCGTCGGCGG ACGAGAGCGT GTCGGATGCG GCAGGGATCG GAGGCGACGG GCCCAACGTG 
AGCTTCCAGA ACGGCGGGGA CACGGTGCTG AGCGAGGGGA GCAGCCTGCA CTCGGGCGGC 
GGCGGCGGCG GCAGTGGGCA CCACCAGCAC TACTACTATG ACACCCACAC CAACACCTAC 
TACCTGCGCA CCTTCGGCCA CAACACTATG GACGCCGTGC CCAGGATCGA CCACTATCGG 
CACACGGCAG CGCAGCTAGG CGAGAAGCTG CTCCGGCCCA GCCTGGCGGA ACTACACGAT 
GAGTTGGAAA AGGAACCTTT TGAGGATGGC TTTGCAAATG GAGAAGAAAG CACGCCAACC 
AGAGAGGCTG TGGTCACATA CACTGCGGAA AGTAAAGGAG TCGTGAAGTT TGGCTGGATT 
AAGGGTGTAC TAGTACGTTG TATGTTAAAC ATTTGGGGAG TGATGCTCTT TATTAGATTG 
TCATGGATTG TGGGTCAAGC TGGAATAGGT CTGTCAGTCA TTGTAATAGC AATGGCCACT 
GTCGTGACAA CTATCACAGG ATTGTCTACA TCAGCAATAG CTACTAATGG GTTTGTACGA 
GGAGGAGGAG CGTATTATTT AATATCTAGA AGTCTAGGGC CAGAATTTGG TGGTGCAATT 
GGCCTGATCT TCGCTTTTGC CAATGCCGTT GCAGTTGCTA TGTACGTGGT TGGATTTGCA 
GAAACTGTGG TGGAGTTGCT TAAGGAACAT TCCATACTTA TGATAGATGA AATCAATGAT 
ATCCGAATTA TTGGAGCCAT TACAGTGGTG ATCCTTTTGG GTATCTCAGT AGCCGGAATG 
GAGTGGGAGG CAAAAGCTCA GATTGTTCTT TTGGTGATTC TACTACTTGC TATAGCTGAT 
TTCGTCATAG GAACATTTAT CCCATTGGAG AGCAAGAAGC CCAAAGGTTT CTTTGGTTAT 
AAGTATGAAA TATTTAGTGA GAACTTTGGG CCAGATTTTC GAGAAGAAGA GACCTTTTTT 
TCTGTATTTG CCATCTTTTT TCCTGCTGCA ACTGGCATTC TGGCTGGAGC AAATATCTCA 
GGTGATCTTG CAGATCCTCA GTCAGCCATA CCCAAAGGAA CACTCTTAGC CATTTTAATC 
ACTACGCTGG TTTACATAGG AATTGCAGTA TCTGTAGGTT CGTGTGTTGT TCGGGACGCT 
ACTGGGAACG TTAATGACAC CATTGTAACA GAGCTGACAA ACTGCACTTC TGCAGCCTGC 
AAGTTAAACT TTGATTTTTC ATCCTGTGAA ACCAACCCTT GTTCCTATGG CCTAATGAAC 
AACTTCCAGG TAATGAGCAT GGTGTCAGGC TTTGCACCAT TAATTTCTGC AGGTATCTTC 
TCAGCCACTC TTTCTTCTGC CTTAGCATCT CTTGTGAGTG CACCCAAAAT ATTTCAGGCT 
CTATGTAAGG ACAACATCTA CCCAGCTTTC CAGATGTTTG CTAAAGGTTA TGGGAAAAAT 
AATGAACCTC TTCGTGGCTA CATCTTAACA TTTTTAATTG CACTTGGATT CATCTTAATT 
GCTGAATTGA ATGTTATTGC TCCAATTATC TCTAACTTCT TCCTTGCATC ATATGCACTG 
ATCAATTTTT CAGTATTTCA TGCATCACTT GCAAAATCTC CAGGGTGGCG TCCTGCATTC 
AAATACTACA ATATGTGGAT ATCACTTCTT GGAGCAATTC TCTGTTGCAT AGTGATGTTT 
GTCATTAACT GGTGGGCTGC ATTGCTAACA TATGTGATAG TTCTTGGACT TTACATTTAT 
GTTACCTACA AAAAACCAGA TGTGAACTGG GGATCCTCCA CACAAGCTCT GACCTACCTG 
AATGCACTGC AGCATTCGAT TCGTCTTTCT GGAGTGGAAG ACCACGTGAA AAACTTCAGG 
CCTCAGTGTC TTGTTATGAC AGGTGCGCCA AACTCACGCC CAGCTTTACT TCATCTTGTT 
CACGATTTCA CAAAAAATGT TGGCTTGATG ATCTGTGGTC ATGTGCATAT GGGCCCTCGA 
AGACAAGCTA TGAAAGAGAT GTCCATTGAT CAAGCCAAAT ATCAGCGGTG GCTTATTAAA 
AACAAAATGA AGGCTTTTTA TGCTCCAGTA CATGCAGATG ATTTGAGAGA GGGTGCACAA 
TATTTGATGC AGGCTGCTGG CCTTGGGCGT ATGAAACCAA ACACACTTGT CCTTGGATTT 
AAGAAAGACT GGTTGCAAGC AGATATGAGG GATGTAGATA TGTATATAAA CTTATTTCAT 
GATGCTTTTG ATATACAATA TGGAGTAGTA GTTATCCGCC TACAAGAAGG TCTGGATATA 
TCTCATCTTC AAGGACAAGA AGAATTACTG TCATCGCAAG AGAAATCTCC TGGTGTAAAG 
GATGTGGTAC TAAGTGTGGA GTATAGTAAA AAATCAGATT TAGATACTTC TAAACCATCC 
AGTGAGAAAT CCATTACACA TAAAGATGAG GAAGAGGATG GCAAGACTCC AACTCAACCA 
CTGTTGAAAA AAGAATCCAA AGGCCCTGCT GTGCCTTTAA ACGTAGCTGA CCAAAAGCTT 
CTTGAAGCTA GTACACAATT TCAGAAAAAA CAGGGAAAGA ATACTATTGA CGTCTGGTGG 
CTTTTTGATG ATGGAGGTTT GACTTTATTG ATACCTTATC TTCTGACTAC CAAGAAAAAG 
TGGAAAGATT GTAAGATCAG AGTATTCATT GGTGGAAAAA TAAATAGAAT AGACCATGAC 
CGAAGAGCGA TGGCTACTTT GCTTAGCAAG TTCCGGATAG ACTTCTCTGA TATCATGGTC 
TTAGGAGATA TTAATACTAA ACCAAAGAAA GAAAATATTG TAGCTTTTGA TGAAATGATT 
GAGCCATACA GACTTCATGA AGATGATAAA GAACAAGATA TTGCAGATAA AATGAAAGAA 
GATGAACCAT GGCGAATAAC AGATAATGAG CTTGAACTTT ATAAGACCAA GACATACCGG 
CAGATCAGGT TAAATGAATT ATTAAAGGAA CATTCAAACA CAGCTAATAT CATTGTCATG 
AGTCTCCCAG TTGCACGAAA AGGTGCTGTG TCTAGTGCTC TCTACATGGC ATGGTTAGAA 
GCTCTATCCA AGGACCTGCC ACCAATTCTC CTAGTCCGTG GGAATCATCA GAGTGTCCTT 
ACCTTCTATT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A2 ( XM_005209087.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209144.1 Bos taurus solute carrier family 12 member 2 (SLC12A2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa235788
Clone ID Related Accession (Same CDS sequence)	XM_005209087.3
Accession Version	XM_005209087.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209087.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG GGCCCACGGC GCCCTCCTCC GGCGCCCCGA GGCTGGCCGG GGTTGGGGAG 
ACGCCTCCAG TCGCCCCGCT GGCCGCCGCC GGGATGGACC TGCCCGGCAC AGCCGTGCCC 
TCAGTGCCGG AGGATGCTGC GGCTGTGAGC CGTGGCGGCG GTGGGGTCCG CGGGGAGGGC 
GCCGCGGCGG CCGGCGACGG ACTGGGCAGA CCCCTGGGGC CCACCCCGAG TCAGAGCCGC 
TTCCAGGTGG ATCTGGTTTC CGAGAATGCC GGGCGGGCGG CGGCGGCGGC GGCTAGCGCT 
GGGGCGGGAG GCAAGGAGAC CCCCGCGGAT GGGAAAGCCA GCGTCGAAAG CGGGCAGGCT 
AAGGCCAGCG AGGAAGCCAA GGGCCGCTTC CGCGTGAACT TCGTGGACCC GGCTGCCTCT 
TCGTCGGCGG ACGAGAGCGT GTCGGATGCG GCAGGGATCG GAGGCGACGG GCCCAACGTG 
AGCTTCCAGA ACGGCGGGGA CACGGTGCTG AGCGAGGGGA GCAGCCTGCA CTCGGGCGGC 
GGCGGCGGCG GCAGTGGGCA CCACCAGCAC TACTACTATG ACACCCACAC CAACACCTAC 
TACCTGCGCA CCTTCGGCCA CAACACTATG GACGCCGTGC CCAGGATCGA CCACTATCGG 
CACACGGCAG CGCAGCTAGG CGAGAAGCTG CTCCGGCCCA GCCTGGCGGA ACTACACGAT 
GAGTTGGAAA AGGAACCTTT TGAGGATGGC TTTGCAAATG GAGAAGAAAG CACGCCAACC 
AGAGAGGCTG TGGTCACATA CACTGCGGAA AGTAAAGGAG TCGTGAAGTT TGGCTGGATT 
AAGGGTGTAC TAGTACGTTG TATGTTAAAC ATTTGGGGAG TGATGCTCTT TATTAGATTG 
TCATGGATTG TGGGTCAAGC TGGAATAGGT CTGTCAGTCA TTGTAATAGC AATGGCCACT 
GTCGTGACAA CTATCACAGG ATTGTCTACA TCAGCAATAG CTACTAATGG GTTTGTACGA 
GGAGGAGGAG CGTATTATTT AATATCTAGA AGTCTAGGGC CAGAATTTGG TGGTGCAATT 
GGCCTGATCT TCGCTTTTGC CAATGCCGTT GCAGTTGCTA TGTACGTGGT TGGATTTGCA 
GAAACTGTGG TGGAGTTGCT TAAGGAACAT TCCATACTTA TGATAGATGA AATCAATGAT 
ATCCGAATTA TTGGAGCCAT TACAGTGGTG ATCCTTTTGG GTATCTCAGT AGCCGGAATG 
GAGTGGGAGG CAAAAGCTCA GATTGTTCTT TTGGTGATTC TACTACTTGC TATAGCTGAT 
TTCGTCATAG GAACATTTAT CCCATTGGAG AGCAAGAAGC CCAAAGGTTT CTTTGGTTAT 
AAGTATGAAA TATTTAGTGA GAACTTTGGG CCAGATTTTC GAGAAGAAGA GACCTTTTTT 
TCTGTATTTG CCATCTTTTT TCCTGCTGCA ACTGGCATTC TGGCTGGAGC AAATATCTCA 
GGTGATCTTG CAGATCCTCA GTCAGCCATA CCCAAAGGAA CACTCTTAGC CATTTTAATC 
ACTACGCTGG TTTACATAGG AATTGCAGTA TCTGTAGGTT CGTGTGTTGT TCGGGACGCT 
ACTGGGAACG TTAATGACAC CATTGTAACA GAGCTGACAA ACTGCACTTC TGCAGCCTGC 
AAGTTAAACT TTGATTTTTC ATCCTGTGAA ACCAACCCTT GTTCCTATGG CCTAATGAAC 
AACTTCCAGG TAATGAGCAT GGTGTCAGGC TTTGCACCAT TAATTTCTGC AGGTATCTTC 
TCAGCCACTC TTTCTTCTGC CTTAGCATCT CTTGTGAGTG CACCCAAAAT ATTTCAGGCT 
CTATGTAAGG ACAACATCTA CCCAGCTTTC CAGATGTTTG CTAAAGGTTA TGGGAAAAAT 
AATGAACCTC TTCGTGGCTA CATCTTAACA TTTTTAATTG CACTTGGATT CATCTTAATT 
GCTGAATTGA ATGTTATTGC TCCAATTATC TCTAACTTCT TCCTTGCATC ATATGCACTG 
ATCAATTTTT CAGTATTTCA TGCATCACTT GCAAAATCTC CAGGGTGGCG TCCTGCATTC 
AAATACTACA ATATGTGGAT ATCACTTCTT GGAGCAATTC TCTGTTGCAT AGTGATGTTT 
GTCATTAACT GGTGGGCTGC ATTGCTAACA TATGTGATAG TTCTTGGACT TTACATTTAT 
GTTACCTACA AAAAACCAGA TGTGAACTGG GGATCCTCCA CACAAGCTCT GACCTACCTG 
AATGCACTGC AGCATTCGAT TCGTCTTTCT GGAGTGGAAG ACCACGTGAA AAACTTCAGG 
CCTCAGTGTC TTGTTATGAC AGGTGCGCCA AACTCACGCC CAGCTTTACT TCATCTTGTT 
CACGATTTCA CAAAAAATGT TGGCTTGATG ATCTGTGGTC ATGTGCATAT GGGCCCTCGA 
AGACAAGCTA TGAAAGAGAT GTCCATTGAT CAAGCCAAAT ATCAGCGGTG GCTTATTAAA 
AACAAAATGA AGGCTTTTTA TGCTCCAGTA CATGCAGATG ATTTGAGAGA GGGTGCACAA 
TATTTGATGC AGGCTGCTGG CCTTGGGCGT ATGAAACCAA ACACACTTGT CCTTGGATTT 
AAGAAAGACT GGTTGCAAGC AGATATGAGG GATGTAGATA TGTATATAAA CTTATTTCAT 
GATGCTTTTG ATATACAATA TGGAGTAGTA GTTATCCGCC TACAAGAAGG TCTGGATATA 
TCTCATCTTC AAGGACAAGA AGAATTACTG TCATCGCAAG AGAAATCTCC TGGTGTAAAG 
GATGTGGTAC TAAGTGTGGA GTATAGTAAA AAATCAGATT TAGATACTTC TAAACCATCC 
AGTGAGAAAT CCATTACACA TAAAGAATCC AAAGGCCCTG CTGTGCCTTT AAACGTAGCT 
GACCAAAAGC TTCTTGAAGC TAGTACACAA TTTCAGAAAA AACAGGGAAA GAATACTATT 
GACGTCTGGT GGCTTTTTGA TGATGGAGGT TTGACTTTAT TGATACCTTA TCTTCTGACT 
ACCAAGAAAA AGTGGAAAGA TTGTAAGATC AGAGTATTCA TTGGTGGAAA AATAAATAGA 
ATAGACCATG ACCGAAGAGC GATGGCTACT TTGCTTAGCA AGTTCCGGAT AGACTTCTCT 
GATATCATGG TCTTAGGAGA TATTAATACT AAACCAAAGA AAGAAAATAT TGTAGCTTTT 
GATGAAATGA TTGAGCCATA CAGACTTCAT GAAGATGATA AAGAACAAGA TATTGCAGAT 
AAAATGAAAG AAGATGAACC ATGGCGAATA ACAGATAATG AGCTTGAACT TTATAAGACC 
AAGACATACC GGCAGATCAG GTTAAATGAA TTATTAAAGG AACATTCAAA CACAGCTAAT 
ATCATTGTCA TGAGTCTCCC AGTTGCACGA AAAGGTGCTG TGTCTAGTGC TCTCTACATG 
GCATGGTTAG AAGCTCTATC CAAGGACCTG CCACCAATTC TCCTAGTCCG TGGGAATCAT 
CAGAGTGTCC TTACCTTCTA TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209144.1
CDS	480..4046
Translation	MEPGPTAPSSGAPRLAGVGETPPVAPLAAAGMDLPGTAVPSVPEDAAAVSRGGGGVRGEGAAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAASAGAGGKETPADGKASVESGQAKASEEAKGRFRVNFVDPAASSSADESVSDAAGIGGDGPNVSFQNGGDTVLSEGSSLHSGGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTREAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVIVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFIPLESKKPKGFFGYKYEIFSENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYIGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCETNPCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLQEGLDISHLQGQEELLSSQEKSPGVKDVVLSVEYSKKSDLDTSKPSSEKSITHKESKGPAVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIVAFDEMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSNTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	XM_005209087.3
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 member 2 (SLC12A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209087.3

ORF Insert Sequence:

ATGGAGCCGG GGCCCACGGC GCCCTCCTCC GGCGCCCCGA GGCTGGCCGG GGTTGGGGAG 
ACGCCTCCAG TCGCCCCGCT GGCCGCCGCC GGGATGGACC TGCCCGGCAC AGCCGTGCCC 
TCAGTGCCGG AGGATGCTGC GGCTGTGAGC CGTGGCGGCG GTGGGGTCCG CGGGGAGGGC 
GCCGCGGCGG CCGGCGACGG ACTGGGCAGA CCCCTGGGGC CCACCCCGAG TCAGAGCCGC 
TTCCAGGTGG ATCTGGTTTC CGAGAATGCC GGGCGGGCGG CGGCGGCGGC GGCTAGCGCT 
GGGGCGGGAG GCAAGGAGAC CCCCGCGGAT GGGAAAGCCA GCGTCGAAAG CGGGCAGGCT 
AAGGCCAGCG AGGAAGCCAA GGGCCGCTTC CGCGTGAACT TCGTGGACCC GGCTGCCTCT 
TCGTCGGCGG ACGAGAGCGT GTCGGATGCG GCAGGGATCG GAGGCGACGG GCCCAACGTG 
AGCTTCCAGA ACGGCGGGGA CACGGTGCTG AGCGAGGGGA GCAGCCTGCA CTCGGGCGGC 
GGCGGCGGCG GCAGTGGGCA CCACCAGCAC TACTACTATG ACACCCACAC CAACACCTAC 
TACCTGCGCA CCTTCGGCCA CAACACTATG GACGCCGTGC CCAGGATCGA CCACTATCGG 
CACACGGCAG CGCAGCTAGG CGAGAAGCTG CTCCGGCCCA GCCTGGCGGA ACTACACGAT 
GAGTTGGAAA AGGAACCTTT TGAGGATGGC TTTGCAAATG GAGAAGAAAG CACGCCAACC 
AGAGAGGCTG TGGTCACATA CACTGCGGAA AGTAAAGGAG TCGTGAAGTT TGGCTGGATT 
AAGGGTGTAC TAGTACGTTG TATGTTAAAC ATTTGGGGAG TGATGCTCTT TATTAGATTG 
TCATGGATTG TGGGTCAAGC TGGAATAGGT CTGTCAGTCA TTGTAATAGC AATGGCCACT 
GTCGTGACAA CTATCACAGG ATTGTCTACA TCAGCAATAG CTACTAATGG GTTTGTACGA 
GGAGGAGGAG CGTATTATTT AATATCTAGA AGTCTAGGGC CAGAATTTGG TGGTGCAATT 
GGCCTGATCT TCGCTTTTGC CAATGCCGTT GCAGTTGCTA TGTACGTGGT TGGATTTGCA 
GAAACTGTGG TGGAGTTGCT TAAGGAACAT TCCATACTTA TGATAGATGA AATCAATGAT 
ATCCGAATTA TTGGAGCCAT TACAGTGGTG ATCCTTTTGG GTATCTCAGT AGCCGGAATG 
GAGTGGGAGG CAAAAGCTCA GATTGTTCTT TTGGTGATTC TACTACTTGC TATAGCTGAT 
TTCGTCATAG GAACATTTAT CCCATTGGAG AGCAAGAAGC CCAAAGGTTT CTTTGGTTAT 
AAGTATGAAA TATTTAGTGA GAACTTTGGG CCAGATTTTC GAGAAGAAGA GACCTTTTTT 
TCTGTATTTG CCATCTTTTT TCCTGCTGCA ACTGGCATTC TGGCTGGAGC AAATATCTCA 
GGTGATCTTG CAGATCCTCA GTCAGCCATA CCCAAAGGAA CACTCTTAGC CATTTTAATC 
ACTACGCTGG TTTACATAGG AATTGCAGTA TCTGTAGGTT CGTGTGTTGT TCGGGACGCT 
ACTGGGAACG TTAATGACAC CATTGTAACA GAGCTGACAA ACTGCACTTC TGCAGCCTGC 
AAGTTAAACT TTGATTTTTC ATCCTGTGAA ACCAACCCTT GTTCCTATGG CCTAATGAAC 
AACTTCCAGG TAATGAGCAT GGTGTCAGGC TTTGCACCAT TAATTTCTGC AGGTATCTTC 
TCAGCCACTC TTTCTTCTGC CTTAGCATCT CTTGTGAGTG CACCCAAAAT ATTTCAGGCT 
CTATGTAAGG ACAACATCTA CCCAGCTTTC CAGATGTTTG CTAAAGGTTA TGGGAAAAAT 
AATGAACCTC TTCGTGGCTA CATCTTAACA TTTTTAATTG CACTTGGATT CATCTTAATT 
GCTGAATTGA ATGTTATTGC TCCAATTATC TCTAACTTCT TCCTTGCATC ATATGCACTG 
ATCAATTTTT CAGTATTTCA TGCATCACTT GCAAAATCTC CAGGGTGGCG TCCTGCATTC 
AAATACTACA ATATGTGGAT ATCACTTCTT GGAGCAATTC TCTGTTGCAT AGTGATGTTT 
GTCATTAACT GGTGGGCTGC ATTGCTAACA TATGTGATAG TTCTTGGACT TTACATTTAT 
GTTACCTACA AAAAACCAGA TGTGAACTGG GGATCCTCCA CACAAGCTCT GACCTACCTG 
AATGCACTGC AGCATTCGAT TCGTCTTTCT GGAGTGGAAG ACCACGTGAA AAACTTCAGG 
CCTCAGTGTC TTGTTATGAC AGGTGCGCCA AACTCACGCC CAGCTTTACT TCATCTTGTT 
CACGATTTCA CAAAAAATGT TGGCTTGATG ATCTGTGGTC ATGTGCATAT GGGCCCTCGA 
AGACAAGCTA TGAAAGAGAT GTCCATTGAT CAAGCCAAAT ATCAGCGGTG GCTTATTAAA 
AACAAAATGA AGGCTTTTTA TGCTCCAGTA CATGCAGATG ATTTGAGAGA GGGTGCACAA 
TATTTGATGC AGGCTGCTGG CCTTGGGCGT ATGAAACCAA ACACACTTGT CCTTGGATTT 
AAGAAAGACT GGTTGCAAGC AGATATGAGG GATGTAGATA TGTATATAAA CTTATTTCAT 
GATGCTTTTG ATATACAATA TGGAGTAGTA GTTATCCGCC TACAAGAAGG TCTGGATATA 
TCTCATCTTC AAGGACAAGA AGAATTACTG TCATCGCAAG AGAAATCTCC TGGTGTAAAG 
GATGTGGTAC TAAGTGTGGA GTATAGTAAA AAATCAGATT TAGATACTTC TAAACCATCC 
AGTGAGAAAT CCATTACACA TAAAGAATCC AAAGGCCCTG CTGTGCCTTT AAACGTAGCT 
GACCAAAAGC TTCTTGAAGC TAGTACACAA TTTCAGAAAA AACAGGGAAA GAATACTATT 
GACGTCTGGT GGCTTTTTGA TGATGGAGGT TTGACTTTAT TGATACCTTA TCTTCTGACT 
ACCAAGAAAA AGTGGAAAGA TTGTAAGATC AGAGTATTCA TTGGTGGAAA AATAAATAGA 
ATAGACCATG ACCGAAGAGC GATGGCTACT TTGCTTAGCA AGTTCCGGAT AGACTTCTCT 
GATATCATGG TCTTAGGAGA TATTAATACT AAACCAAAGA AAGAAAATAT TGTAGCTTTT 
GATGAAATGA TTGAGCCATA CAGACTTCAT GAAGATGATA AAGAACAAGA TATTGCAGAT 
AAAATGAAAG AAGATGAACC ATGGCGAATA ACAGATAATG AGCTTGAACT TTATAAGACC 
AAGACATACC GGCAGATCAG GTTAAATGAA TTATTAAAGG AACATTCAAA CACAGCTAAT 
ATCATTGTCA TGAGTCTCCC AGTTGCACGA AAAGGTGCTG TGTCTAGTGC TCTCTACATG 
GCATGGTTAG AAGCTCTATC CAAGGACCTG CCACCAATTC TCCTAGTCCG TGGGAATCAT 
CAGAGTGTCC TTACCTTCTA TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A2 ( NM_174782.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777207.1 Bos taurus solute carrier family 12 member 2 (SLC12A2), mRNA.

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CloneID	OBa38982
Clone ID Related Accession (Same CDS sequence)	NM_174782.3
Accession Version	NM_174782.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U70138.1. On Aug 30, 2012 this sequence version replaced NM_174782.2. ##Evidence-Data-START## Transcript exon combination :: U70138.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCCGG GGCCCACGGC GCCCTCCTCC GGCGCCCCGA GGCTGGCCGG GGTTGGGGAG 
ACGCCTCCAG TCGCACCGCT GGCCGCCGCC GGGATGGACC TGCCCGGCAC AGCCGTGCCC 
TCAGTGCCGG AGGATGCTGC GGCTGTGAGC CGTGGCGGCG GTGGGGTCCG CGGGGAGGGC 
GCCGCGGCGG CCGGCGACGG ACTGGGCAGA CCCCTGGGGC CCACCCCGAG TCAGAGCCGC 
TTCCAGGTGG ATCTGGTTTC CGAGAATGCC GGGCGGGCGG CGGCGGCGGC GGCTAGCGCT 
GGGGCGGGAG GCAAGGAGAC CCCCGCGGAT GGGAAAGCCA GCGTCGAAAG CGGGCAGGCT 
AAGGCCAGCG AGGAAGCCAA GGGCCGCTTC CGCGTGAACT TCGTGGACCC GGCTGCCTCT 
TCGTCGGCGG ACGAGAGCGT GTCGGATGCG GCAGGGATCG GAGGCGACGG GCCCAACGTG 
AGCTTCCAGA ACGGCGGGGA CACGGTGCTG AGCGAGGGGA GCAGCCTGCA CTCGGGCGGC 
GGCGGCGGCG GCAGTGGGCA CCACCAGCAC TACTACTATG ACACCCACAC CAACACCTAC 
TACCTGCGCA CCTTCGGCCA CAACACTATG GACGCCGTGC CCAGGATCGA CCACTATCGG 
CACACGGCAG CGCAGCTAGG CGAGAAGCTG CTCCGGCCCA GCCTGGCGGA ACTACACGAT 
GAGTTGGAAA AGGAACCTTT TGAGGATGGC TTTGCAAATG GAGAAGAAAG CACGCCAACC 
AGAGAGGCTG TGGTCACATA CACTGCGGAA AGTAAAGGAG TCGTGAAGTT TGGCTGGATT 
AAGGGTGTAC TAGTACGTTG TATGTTAAAC ATTTGGGGAG TGATGCTCTT TATTAGATTG 
TCATGGATTG TGGGTCAAGC TGGAATAGGT CTGTCAGTCA TTGTAATAGC AATGGCCACT 
GTCGTGACAA CTATCACAGG ATTGTCTACA TCAGCAATAG CTACTAATGG GTTTGTACGA 
GGAGGAGGAG CGTATTATTT AATATCTAGA AGTCTAGGGC CAGAATTTGG TGGTGCAATT 
GGCCTGATCT TCGCTTTTGC CAATGCCGTT GCAGTTGCTA TGTACGTGGT TGGATTTGCA 
GAAACTGTGG TGGAGTTGCT TAAGGAACAT TCCATACTTA TGATAGATGA AATCAATGAT 
ATCCGAATTA TTGGAGCCAT TACAGTGGTG ATCCTTTTGG GTATCTCAGT AGCCGGAATG 
GAGTGGGAGG CAAAAGCTCA GATTGTTCTT TTGGTGATTC TACTACTTGC TATAGCTGAT 
TTCGTCATAG GAACATTTAT CCCATTGGAG AGCAAGAAGC CCAAAGGTTT CTTTGGTTAT 
AAGTATGAAA TATTTAGTGA GAACTTTGGG CCAGATTTTC GAGAAGAAGA GACCTTTTTT 
TCTGTATTTG CCATCTTTTT TCCTGCTGCA ACTGGCATTC TGGCTGGAGC AAATATCTCA 
GGTGATCTTG CAGATCCTCA GTCAGCCATA CCCAAAGGAA CACCCTTAGC CATTTTAATC 
ACTACGCTGG TTTACATAGG AATTGCAGTA TCTGTAGGTT CGTGTGTTGT TCGGGACGCT 
ACTGGGAACG TTAATGACAC CATTGTAACA GAGCTGACAA ACTGCACTTC TGCAGCCTGC 
AAGTTAAACT TTGATTTTTC ATCCTGTGAA ACCAACCCTT GTTCCTATGG CCTAATGAAC 
AACTTCCAGG TAATGAGCAT GGTGTCAGGC TTTGCACCAT TAATTTCTGC AGGTATCTTC 
TCAGCCACTC TTTCTTCTGC CTTAGCATCT CTTGTGAGTG CACCCAAAAT ATTTCAGGCT 
CTATGTAAGG ACAACATCTA CCCAGCTTTC CAGATGTTTG CTAAAGGTTA TGGGAAAAAT 
AATGAACCTC TTCGTGGCTA CATCTTAACA TTTTTAATTG CACTTGGATT CATCTTAATT 
GCTGAATTGA ATGTTATTGC TCCAATTATC TCTAACTTCT TCCTTGCATC ATATGCACTG 
ATCAATTTTT CAGTATTTCA TGCATCACTT GCAAAATCTC CAGGGTGGCG TCCTGCATTC 
AAATACTACA ATATGTGGAT ATCACTTCTT GGAGCAATTC TCTGTTGCAT AGTGATGTTT 
GTCATTAACT GGTGGGCTGC ATTGCTAACA TATGTGATAG TTCTTGGACT TTACATTTAT 
GTTACCTACA AAAAACCAGA TGTGAACTGG GGATCCTCCA CACAAGCTCT GACCTACCTG 
AATGCACTGC AGCATTCGAT TCGTCTTTCT GGAGTGGAAG ACCACGTGAA AAACTTCAGG 
CCTCAGTGTC TTGTTATGAC AGGTGCGCCA AACTCACGCC CAGCTTTACT TCATCTTGTT 
CACGATTTCA CAAAAAATGT TGGCTTGATG ATCTGTGGTC ATGTGCATAT GGGCCCTCGA 
AGACAAGCTA TGAAAGAGAT GTCCATTGAT CAAGCCAAAT ATCAGCGGTG GCTTATTAAA 
AACAAAATGA AGGCTTTTTA TGCTCCAGTA CATGCAGATG ATTTGAGAGA GGGTGCACAA 
TATTTGATGC AGGCTGCTGG CCTTGGGCGT ATGAAACCAA ACACACTTGT CCTTGGATTT 
AAGAAAGACT GGTTGCAAGC AGATATGAGG GATGTAGATA TGTATATAAA CTTATTTCAT 
GATGCTTTTG ATATACAATA TGGAGTAGTA GTTATCCGCC TACAAGAAGG TCTGGATATA 
TCTCATCTTC AAGGACAAGA AGAATTACTG TCATCGCAAG AGAAATCTCC TGGTGTAAAG 
GATGTGGTAC TAAGTGTGGA GTATAGTAAA AAATCAGATT TAGATACTTC TAAACCATCC 
AGTGAGAAAT CCATTACACA TAAAGAGGAT GGCAAGACTC CAATTCAACC ACTGTTGAAA 
AAAGAATCCA AAGGCCCTGC TGTGCCTTTA AACGTAGCTG ACCAAAAGCT TCTTGAAGCT 
AGTACACAAT TTCAGAAAAA ACAGGGAAAG AATACTATTG ACGTCTGGTG GCTTTTTGAT 
GATGGAGGTT TGACTTTATT GATACCTTAT CTTCTGACTA CCAAGAAAAA GTGGAAAGAT 
TGTAAGATCA GAGTATTCAT TGGTGGAAAA ATAAATAGAA TAGACCATGA CCGAAGAGCG 
ATGCCTACTT TGCTTACCAA GTTCCGGATA GACTTCTCTG ATATCATGGT CTTAGGAGAT 
ATTAATACTA AACCAAAGAA AGAAAATATT GTAGCTTTTG ATGAAATGAT TGAGCCATAC 
AGACTTCATG AAGATGATAA AGAACAAGAT ATTGCAGATA AAATGAAAGA AGATGAACCA 
TGGCGAATAA CAGATAATGA GCTTGAACTT TATAAGACCA AGACATACCG GCAGATCAGG 
TTAAATGAAT TATTAAAGGA ACATTCAAAC ACAGCTAATA TCATTGTCAT GAGTCTCCCA 
GTTGCACGAA AAGGTGCTGT GTCTAGTGCT CTCTACATGG CATGGTTAGA AGCTCTATCC 
AAGGACCTGC CACCAATTCT CCTAGTCCGT GGGAATCATC AGAGTGTCCT TACCTTCTAT 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777207.1
CDS	483..4088
Translation	MEPGPTAPSSGAPRLAGVGETPPVAPLAAAGMDLPGTAVPSVPEDAAAVSRGGGGVRGEGAAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAASAGAGGKETPADGKASVESGQAKASEEAKGRFRVNFVDPAASSSADESVSDAAGIGGDGPNVSFQNGGDTVLSEGSSLHSGGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTREAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVIVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFIPLESKKPKGFFGYKYEIFSENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTPLAILITTLVYIGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCETNPCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLQEGLDISHLQGQEELLSSQEKSPGVKDVVLSVEYSKKSDLDTSKPSSEKSITHKEDGKTPIQPLLKKESKGPAVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMPTLLTKFRIDFSDIMVLGDINTKPKKENIVAFDEMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSNTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	NM_174782.3
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 member 2 (SLC12A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174782.3

ORF Insert Sequence:

ATGGAGCCGG GGCCCACGGC GCCCTCCTCC GGCGCCCCGA GGCTGGCCGG GGTTGGGGAG 
ACGCCTCCAG TCGCACCGCT GGCCGCCGCC GGGATGGACC TGCCCGGCAC AGCCGTGCCC 
TCAGTGCCGG AGGATGCTGC GGCTGTGAGC CGTGGCGGCG GTGGGGTCCG CGGGGAGGGC 
GCCGCGGCGG CCGGCGACGG ACTGGGCAGA CCCCTGGGGC CCACCCCGAG TCAGAGCCGC 
TTCCAGGTGG ATCTGGTTTC CGAGAATGCC GGGCGGGCGG CGGCGGCGGC GGCTAGCGCT 
GGGGCGGGAG GCAAGGAGAC CCCCGCGGAT GGGAAAGCCA GCGTCGAAAG CGGGCAGGCT 
AAGGCCAGCG AGGAAGCCAA GGGCCGCTTC CGCGTGAACT TCGTGGACCC GGCTGCCTCT 
TCGTCGGCGG ACGAGAGCGT GTCGGATGCG GCAGGGATCG GAGGCGACGG GCCCAACGTG 
AGCTTCCAGA ACGGCGGGGA CACGGTGCTG AGCGAGGGGA GCAGCCTGCA CTCGGGCGGC 
GGCGGCGGCG GCAGTGGGCA CCACCAGCAC TACTACTATG ACACCCACAC CAACACCTAC 
TACCTGCGCA CCTTCGGCCA CAACACTATG GACGCCGTGC CCAGGATCGA CCACTATCGG 
CACACGGCAG CGCAGCTAGG CGAGAAGCTG CTCCGGCCCA GCCTGGCGGA ACTACACGAT 
GAGTTGGAAA AGGAACCTTT TGAGGATGGC TTTGCAAATG GAGAAGAAAG CACGCCAACC 
AGAGAGGCTG TGGTCACATA CACTGCGGAA AGTAAAGGAG TCGTGAAGTT TGGCTGGATT 
AAGGGTGTAC TAGTACGTTG TATGTTAAAC ATTTGGGGAG TGATGCTCTT TATTAGATTG 
TCATGGATTG TGGGTCAAGC TGGAATAGGT CTGTCAGTCA TTGTAATAGC AATGGCCACT 
GTCGTGACAA CTATCACAGG ATTGTCTACA TCAGCAATAG CTACTAATGG GTTTGTACGA 
GGAGGAGGAG CGTATTATTT AATATCTAGA AGTCTAGGGC CAGAATTTGG TGGTGCAATT 
GGCCTGATCT TCGCTTTTGC CAATGCCGTT GCAGTTGCTA TGTACGTGGT TGGATTTGCA 
GAAACTGTGG TGGAGTTGCT TAAGGAACAT TCCATACTTA TGATAGATGA AATCAATGAT 
ATCCGAATTA TTGGAGCCAT TACAGTGGTG ATCCTTTTGG GTATCTCAGT AGCCGGAATG 
GAGTGGGAGG CAAAAGCTCA GATTGTTCTT TTGGTGATTC TACTACTTGC TATAGCTGAT 
TTCGTCATAG GAACATTTAT CCCATTGGAG AGCAAGAAGC CCAAAGGTTT CTTTGGTTAT 
AAGTATGAAA TATTTAGTGA GAACTTTGGG CCAGATTTTC GAGAAGAAGA GACCTTTTTT 
TCTGTATTTG CCATCTTTTT TCCTGCTGCA ACTGGCATTC TGGCTGGAGC AAATATCTCA 
GGTGATCTTG CAGATCCTCA GTCAGCCATA CCCAAAGGAA CACCCTTAGC CATTTTAATC 
ACTACGCTGG TTTACATAGG AATTGCAGTA TCTGTAGGTT CGTGTGTTGT TCGGGACGCT 
ACTGGGAACG TTAATGACAC CATTGTAACA GAGCTGACAA ACTGCACTTC TGCAGCCTGC 
AAGTTAAACT TTGATTTTTC ATCCTGTGAA ACCAACCCTT GTTCCTATGG CCTAATGAAC 
AACTTCCAGG TAATGAGCAT GGTGTCAGGC TTTGCACCAT TAATTTCTGC AGGTATCTTC 
TCAGCCACTC TTTCTTCTGC CTTAGCATCT CTTGTGAGTG CACCCAAAAT ATTTCAGGCT 
CTATGTAAGG ACAACATCTA CCCAGCTTTC CAGATGTTTG CTAAAGGTTA TGGGAAAAAT 
AATGAACCTC TTCGTGGCTA CATCTTAACA TTTTTAATTG CACTTGGATT CATCTTAATT 
GCTGAATTGA ATGTTATTGC TCCAATTATC TCTAACTTCT TCCTTGCATC ATATGCACTG 
ATCAATTTTT CAGTATTTCA TGCATCACTT GCAAAATCTC CAGGGTGGCG TCCTGCATTC 
AAATACTACA ATATGTGGAT ATCACTTCTT GGAGCAATTC TCTGTTGCAT AGTGATGTTT 
GTCATTAACT GGTGGGCTGC ATTGCTAACA TATGTGATAG TTCTTGGACT TTACATTTAT 
GTTACCTACA AAAAACCAGA TGTGAACTGG GGATCCTCCA CACAAGCTCT GACCTACCTG 
AATGCACTGC AGCATTCGAT TCGTCTTTCT GGAGTGGAAG ACCACGTGAA AAACTTCAGG 
CCTCAGTGTC TTGTTATGAC AGGTGCGCCA AACTCACGCC CAGCTTTACT TCATCTTGTT 
CACGATTTCA CAAAAAATGT TGGCTTGATG ATCTGTGGTC ATGTGCATAT GGGCCCTCGA 
AGACAAGCTA TGAAAGAGAT GTCCATTGAT CAAGCCAAAT ATCAGCGGTG GCTTATTAAA 
AACAAAATGA AGGCTTTTTA TGCTCCAGTA CATGCAGATG ATTTGAGAGA GGGTGCACAA 
TATTTGATGC AGGCTGCTGG CCTTGGGCGT ATGAAACCAA ACACACTTGT CCTTGGATTT 
AAGAAAGACT GGTTGCAAGC AGATATGAGG GATGTAGATA TGTATATAAA CTTATTTCAT 
GATGCTTTTG ATATACAATA TGGAGTAGTA GTTATCCGCC TACAAGAAGG TCTGGATATA 
TCTCATCTTC AAGGACAAGA AGAATTACTG TCATCGCAAG AGAAATCTCC TGGTGTAAAG 
GATGTGGTAC TAAGTGTGGA GTATAGTAAA AAATCAGATT TAGATACTTC TAAACCATCC 
AGTGAGAAAT CCATTACACA TAAAGAGGAT GGCAAGACTC CAATTCAACC ACTGTTGAAA 
AAAGAATCCA AAGGCCCTGC TGTGCCTTTA AACGTAGCTG ACCAAAAGCT TCTTGAAGCT 
AGTACACAAT TTCAGAAAAA ACAGGGAAAG AATACTATTG ACGTCTGGTG GCTTTTTGAT 
GATGGAGGTT TGACTTTATT GATACCTTAT CTTCTGACTA CCAAGAAAAA GTGGAAAGAT 
TGTAAGATCA GAGTATTCAT TGGTGGAAAA ATAAATAGAA TAGACCATGA CCGAAGAGCG 
ATGCCTACTT TGCTTACCAA GTTCCGGATA GACTTCTCTG ATATCATGGT CTTAGGAGAT 
ATTAATACTA AACCAAAGAA AGAAAATATT GTAGCTTTTG ATGAAATGAT TGAGCCATAC 
AGACTTCATG AAGATGATAA AGAACAAGAT ATTGCAGATA AAATGAAAGA AGATGAACCA 
TGGCGAATAA CAGATAATGA GCTTGAACTT TATAAGACCA AGACATACCG GCAGATCAGG 
TTAAATGAAT TATTAAAGGA ACATTCAAAC ACAGCTAATA TCATTGTCAT GAGTCTCCCA 
GTTGCACGAA AAGGTGCTGT GTCTAGTGCT CTCTACATGG CATGGTTAGA AGCTCTATCC 
AAGGACCTGC CACCAATTCT CCTAGTCCGT GGGAATCATC AGAGTGTCCT TACCTTCTAT 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A2 ( XM_005209086.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209143.1 Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa21259
Clone ID Related Accession (Same CDS sequence)	XM_005209086.2
Accession Version	XM_005209086.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104020.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005209086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG GGCCCACGGC GCCCTCCTCC GGCGCCCCGA GGCTGGCCGG GGTTGGGGAG 
ACGCCTCCAG TCGCCCCGCT GGCCGCCGCC GGGATGGACC TGCCCGGCAC AGCCGTGCCC 
TCAGTGCCGG AGGATGCTGC GGCTGTGAGC CGTGGCGGCG GTGGGGTCCG CGGGGAGGGC 
GCCGCGGCGG CCGGCGACGG ACTGGGCAGA CCCCTGGGGC CCACCCCGAG TCAGAGCCGC 
TTCCAGGTGG ATCTGGTTTC CGAGAATGCC GGGCGGGCGG CGGCGGCGGC GGCTAGCGCT 
GGGGCGGGAG GCAAGGAGAC CCCCGCGGAT GGGAAAGCCA GCGTCGAAAG CGGGCAGGCT 
AAGGCCAGCG AGGAAGCCAA GGGCCGCTTC CGCGTGAACT TCGTGGACCC GGCTGCCTCT 
TCGTCGGCGG ACGAGAGCGT GTCGGATGCG GCAGGGATCG GAGGCGACGG GCCCAACGTG 
AGCTTCCAGA ACGGCGGGGA CACGGTGCTG AGCGAGGGGA GCAGCCTGCA CTCGGGCGGC 
GGCGGCGGCG GCAGTGGGCA CCACCAGCAC TACTACTATG ACACCCACAC CAACACCTAC 
TACCTGCGCA CCTTCGGCCA CAACACTATG GACGCCGTGC CCAGGATCGA CCACTATCGG 
CACACGGCAG CGCAGCTAGG CGAGAAGCTG CTCCGGCCCA GCCTGGCGGA ACTACACGAT 
GAGTTGGAAA AGGAACCTTT TGAGGATGGC TTTGCAAATG GAGAAGAAAG CACGCCAACC 
AGAGAGGCTG TGGTCACATA CACTGCGGAA AGTAAAGGAG TCGTGAAGTT TGGCTGGATT 
AAGGGTGTAC TAGTACGTTG TATGTTAAAC ATTTGGGGAG TGATGCTCTT TATTAGATTG 
TCATGGATTG TGGGTCAAGC TGGAATAGGT CTGTCAGTCA TTGTAATAGC AATGGCCACT 
GTCGTGACAA CTATCACAGG ATTGTCTACA TCAGCAATAG CTACTAATGG GTTTGTACGA 
GGAGGAGGAG CGTATTATTT AATATCTAGA AGTCTAGGGC CAGAATTTGG TGGTGCAATT 
GGCCTGATCT TCGCTTTTGC CAATGCCGTT GCAGTTGCTA TGTACGTGGT TGGATTTGCA 
GAAACTGTGG TGGAGTTGCT TAAGGAACAT TCCATACTTA TGATAGATGA AATCAATGAT 
ATCCGAATTA TTGGAGCCAT TACAGTGGTG ATCCTTTTGG GTATCTCAGT AGCCGGAATG 
GAGTGGGAGG CAAAAGCTCA GATTGTTCTT TTGGTGATTC TACTACTTGC TATAGCTGAT 
TTCGTCATAG GAACATTTAT CCCATTGGAG AGCAAGAAGC CCAAAGGTTT CTTTGGTTAT 
AAGTATGAAA TATTTAGTGA GAACTTTGGG CCAGATTTTC GAGAAGAAGA GACCTTTTTT 
TCTGTATTTG CCATCTTTTT TCCTGCTGCA ACTGGCATTC TGGCTGGAGC AAATATCTCA 
GGTGATCTTG CAGATCCTCA GTCAGCCATA CCCAAAGGAA CACTCTTAGC CATTTTAATC 
ACTACGCTGG TTTACATAGG AATTGCAGTA TCTGTAGGTT CGTGTGTTGT TCGGGACGCT 
ACTGGGAACG TTAATGACAC CATTGTAACA GAGCTGACAA ACTGCACTTC TGCAGCCTGC 
AAGTTAAACT TTGATTTTTC ATCCTGTGAA ACCAACCCTT GTTCCTATGG CCTAATGAAC 
AACTTCCAGG TAATGAGCAT GGTGTCAGGC TTTGCACCAT TAATTTCTGC AGGTATCTTC 
TCAGCCACTC TTTCTTCTGC CTTAGCATCT CTTGTGAGTG CACCCAAAAT ATTTCAGGCT 
CTATGTAAGG ACAACATCTA CCCAGCTTTC CAGATGTTTG CTAAAGGTTA TGGGAAAAAT 
AATGAACCTC TTCGTGGCTA CATCTTAACA TTTTTAATTG CACTTGGATT CATCTTAATT 
GCTGAATTGA ATGTTATTGC TCCAATTATC TCTAACTTCT TCCTTGCATC ATATGCACTG 
ATCAATTTTT CAGTATTTCA TGCATCACTT GCAAAATCTC CAGGGTGGCG TCCTGCATTC 
AAATACTACA ATATGTGGAT ATCACTTCTT GGAGCAATTC TCTGTTGCAT AGTGATGTTT 
GTCATTAACT GGTGGGCTGC ATTGCTAACA TATGTGATAG TTCTTGGACT TTACATTTAT 
GTTACCTACA AAAAACCAGA TGTGAACTGG GGATCCTCCA CACAAGCTCT GACCTACCTG 
AATGCACTGC AGCATTCGAT TCGTCTTTCT GGAGTGGAAG ACCACGTGAA AAACTTCAGG 
CCTCAGTGTC TTGTTATGAC AGGTGCGCCA AACTCACGCC CAGCTTTACT TCATCTTGTT 
CACGATTTCA CAAAAAATGT TGGCTTGATG ATCTGTGGTC ATGTGCATAT GGGCCCTCGA 
AGACAAGCTA TGAAAGAGAT GTCCATTGAT CAAGCCAAAT ATCAGCGGTG GCTTATTAAA 
AACAAAATGA AGGCTTTTTA TGCTCCAGTA CATGCAGATG ATTTGAGAGA GGGTGCACAA 
TATTTGATGC AGGCTGCTGG CCTTGGGCGT ATGAAACCAA ACACACTTGT CCTTGGATTT 
AAGAAAGACT GGTTGCAAGC AGATATGAGG GATGTAGATA TGTATATAAA CTTATTTCAT 
GATGCTTTTG ATATACAATA TGGAGTAGTA GTTATCCGCC TACAAGAAGG TCTGGATATA 
TCTCATCTTC AAGGACAAGA AGAATTACTA TCATCGCAAG AGAAATCTCC TGGTGTAAAG 
GATGTGGTAC TAAGTGTGGA GTATAGTAAA AAATCAGATT TAGATACTTC TAAACCATCC 
AGTGAGAAAT CCATTACACA TAAAGATGAG GAAGAGGATG GCAAGACTCC AACTCAACCA 
CTGTTGAAAA AAGAATCCAA AGGCCCTGCT GTGCCTTTAA ACGTAGCTGA CCAAAAGCTT 
CTTGAAGCTA GTACACAATT TCAGAAAAAA CAGGGAAAGA ATACTATTGA CGTCTGGTGG 
CTTTTTGATG ATGGAGGTTT GACTTTATTG ATACCTTATC TTCTGACTAC CAAGAAAAAG 
TGGAAAGATT GTAAGATCAG AGTATTCATT GGTGGAAAAA TAAATAGAAT AGACCATGAC 
CGAAGAGCGA TGGCTACTTT GCTTAGCAAG TTCCGGATAG ACTTCTCTGA TATCATGGTC 
TTAGGAGATA TTAATACTAA ACCAAAGAAA GAAAATATTG TAGCTTTTGA TGAAATGATT 
GAGCCATACA GACTTCATGA AGATGATAAA GAACAAGATA TTGCAGATAA AATGAAAGAA 
GATGAACCAT GGCGAATAAC AGATAATGAG CTTGAACTTT ATAAGACCAA GACATACCGG 
CAGATCAGGT TAAATGAATT ATTAAAGGAA CATTCAAACA CAGCTAATAT CATTGTCATG 
AGTCTCCCAG TTGCACGAAA AGGTGCTGTG TCTAGTGCTC TCTACATGGC ATGGTTAGAA 
GCTCTATCCA AGGACCTGCC ACCAATTCTC CTAGTCCGTG GGAATCATCA GAGTGTCCTT 
ACCTTCTATT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209143.1
CDS	480..4094
Translation	MEPGPTAPSSGAPRLAGVGETPPVAPLAAAGMDLPGTAVPSVPEDAAAVSRGGGGVRGEGAAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAASAGAGGKETPADGKASVESGQAKASEEAKGRFRVNFVDPAASSSADESVSDAAGIGGDGPNVSFQNGGDTVLSEGSSLHSGGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTREAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVIVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFIPLESKKPKGFFGYKYEIFSENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYIGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCETNPCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLQEGLDISHLQGQEELLSSQEKSPGVKDVVLSVEYSKKSDLDTSKPSSEKSITHKDEEEDGKTPTQPLLKKESKGPAVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIVAFDEMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSNTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	XM_005209086.2
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209086.2

ORF Insert Sequence:

ATGGAGCCGG GGCCCACGGC GCCCTCCTCC GGCGCCCCGA GGCTGGCCGG GGTTGGGGAG 
ACGCCTCCAG TCGCCCCGCT GGCCGCCGCC GGGATGGACC TGCCCGGCAC AGCCGTGCCC 
TCAGTGCCGG AGGATGCTGC GGCTGTGAGC CGTGGCGGCG GTGGGGTCCG CGGGGAGGGC 
GCCGCGGCGG CCGGCGACGG ACTGGGCAGA CCCCTGGGGC CCACCCCGAG TCAGAGCCGC 
TTCCAGGTGG ATCTGGTTTC CGAGAATGCC GGGCGGGCGG CGGCGGCGGC GGCTAGCGCT 
GGGGCGGGAG GCAAGGAGAC CCCCGCGGAT GGGAAAGCCA GCGTCGAAAG CGGGCAGGCT 
AAGGCCAGCG AGGAAGCCAA GGGCCGCTTC CGCGTGAACT TCGTGGACCC GGCTGCCTCT 
TCGTCGGCGG ACGAGAGCGT GTCGGATGCG GCAGGGATCG GAGGCGACGG GCCCAACGTG 
AGCTTCCAGA ACGGCGGGGA CACGGTGCTG AGCGAGGGGA GCAGCCTGCA CTCGGGCGGC 
GGCGGCGGCG GCAGTGGGCA CCACCAGCAC TACTACTATG ACACCCACAC CAACACCTAC 
TACCTGCGCA CCTTCGGCCA CAACACTATG GACGCCGTGC CCAGGATCGA CCACTATCGG 
CACACGGCAG CGCAGCTAGG CGAGAAGCTG CTCCGGCCCA GCCTGGCGGA ACTACACGAT 
GAGTTGGAAA AGGAACCTTT TGAGGATGGC TTTGCAAATG GAGAAGAAAG CACGCCAACC 
AGAGAGGCTG TGGTCACATA CACTGCGGAA AGTAAAGGAG TCGTGAAGTT TGGCTGGATT 
AAGGGTGTAC TAGTACGTTG TATGTTAAAC ATTTGGGGAG TGATGCTCTT TATTAGATTG 
TCATGGATTG TGGGTCAAGC TGGAATAGGT CTGTCAGTCA TTGTAATAGC AATGGCCACT 
GTCGTGACAA CTATCACAGG ATTGTCTACA TCAGCAATAG CTACTAATGG GTTTGTACGA 
GGAGGAGGAG CGTATTATTT AATATCTAGA AGTCTAGGGC CAGAATTTGG TGGTGCAATT 
GGCCTGATCT TCGCTTTTGC CAATGCCGTT GCAGTTGCTA TGTACGTGGT TGGATTTGCA 
GAAACTGTGG TGGAGTTGCT TAAGGAACAT TCCATACTTA TGATAGATGA AATCAATGAT 
ATCCGAATTA TTGGAGCCAT TACAGTGGTG ATCCTTTTGG GTATCTCAGT AGCCGGAATG 
GAGTGGGAGG CAAAAGCTCA GATTGTTCTT TTGGTGATTC TACTACTTGC TATAGCTGAT 
TTCGTCATAG GAACATTTAT CCCATTGGAG AGCAAGAAGC CCAAAGGTTT CTTTGGTTAT 
AAGTATGAAA TATTTAGTGA GAACTTTGGG CCAGATTTTC GAGAAGAAGA GACCTTTTTT 
TCTGTATTTG CCATCTTTTT TCCTGCTGCA ACTGGCATTC TGGCTGGAGC AAATATCTCA 
GGTGATCTTG CAGATCCTCA GTCAGCCATA CCCAAAGGAA CACTCTTAGC CATTTTAATC 
ACTACGCTGG TTTACATAGG AATTGCAGTA TCTGTAGGTT CGTGTGTTGT TCGGGACGCT 
ACTGGGAACG TTAATGACAC CATTGTAACA GAGCTGACAA ACTGCACTTC TGCAGCCTGC 
AAGTTAAACT TTGATTTTTC ATCCTGTGAA ACCAACCCTT GTTCCTATGG CCTAATGAAC 
AACTTCCAGG TAATGAGCAT GGTGTCAGGC TTTGCACCAT TAATTTCTGC AGGTATCTTC 
TCAGCCACTC TTTCTTCTGC CTTAGCATCT CTTGTGAGTG CACCCAAAAT ATTTCAGGCT 
CTATGTAAGG ACAACATCTA CCCAGCTTTC CAGATGTTTG CTAAAGGTTA TGGGAAAAAT 
AATGAACCTC TTCGTGGCTA CATCTTAACA TTTTTAATTG CACTTGGATT CATCTTAATT 
GCTGAATTGA ATGTTATTGC TCCAATTATC TCTAACTTCT TCCTTGCATC ATATGCACTG 
ATCAATTTTT CAGTATTTCA TGCATCACTT GCAAAATCTC CAGGGTGGCG TCCTGCATTC 
AAATACTACA ATATGTGGAT ATCACTTCTT GGAGCAATTC TCTGTTGCAT AGTGATGTTT 
GTCATTAACT GGTGGGCTGC ATTGCTAACA TATGTGATAG TTCTTGGACT TTACATTTAT 
GTTACCTACA AAAAACCAGA TGTGAACTGG GGATCCTCCA CACAAGCTCT GACCTACCTG 
AATGCACTGC AGCATTCGAT TCGTCTTTCT GGAGTGGAAG ACCACGTGAA AAACTTCAGG 
CCTCAGTGTC TTGTTATGAC AGGTGCGCCA AACTCACGCC CAGCTTTACT TCATCTTGTT 
CACGATTTCA CAAAAAATGT TGGCTTGATG ATCTGTGGTC ATGTGCATAT GGGCCCTCGA 
AGACAAGCTA TGAAAGAGAT GTCCATTGAT CAAGCCAAAT ATCAGCGGTG GCTTATTAAA 
AACAAAATGA AGGCTTTTTA TGCTCCAGTA CATGCAGATG ATTTGAGAGA GGGTGCACAA 
TATTTGATGC AGGCTGCTGG CCTTGGGCGT ATGAAACCAA ACACACTTGT CCTTGGATTT 
AAGAAAGACT GGTTGCAAGC AGATATGAGG GATGTAGATA TGTATATAAA CTTATTTCAT 
GATGCTTTTG ATATACAATA TGGAGTAGTA GTTATCCGCC TACAAGAAGG TCTGGATATA 
TCTCATCTTC AAGGACAAGA AGAATTACTA TCATCGCAAG AGAAATCTCC TGGTGTAAAG 
GATGTGGTAC TAAGTGTGGA GTATAGTAAA AAATCAGATT TAGATACTTC TAAACCATCC 
AGTGAGAAAT CCATTACACA TAAAGATGAG GAAGAGGATG GCAAGACTCC AACTCAACCA 
CTGTTGAAAA AAGAATCCAA AGGCCCTGCT GTGCCTTTAA ACGTAGCTGA CCAAAAGCTT 
CTTGAAGCTA GTACACAATT TCAGAAAAAA CAGGGAAAGA ATACTATTGA CGTCTGGTGG 
CTTTTTGATG ATGGAGGTTT GACTTTATTG ATACCTTATC TTCTGACTAC CAAGAAAAAG 
TGGAAAGATT GTAAGATCAG AGTATTCATT GGTGGAAAAA TAAATAGAAT AGACCATGAC 
CGAAGAGCGA TGGCTACTTT GCTTAGCAAG TTCCGGATAG ACTTCTCTGA TATCATGGTC 
TTAGGAGATA TTAATACTAA ACCAAAGAAA GAAAATATTG TAGCTTTTGA TGAAATGATT 
GAGCCATACA GACTTCATGA AGATGATAAA GAACAAGATA TTGCAGATAA AATGAAAGAA 
GATGAACCAT GGCGAATAAC AGATAATGAG CTTGAACTTT ATAAGACCAA GACATACCGG 
CAGATCAGGT TAAATGAATT ATTAAAGGAA CATTCAAACA CAGCTAATAT CATTGTCATG 
AGTCTCCCAG TTGCACGAAA AGGTGCTGTG TCTAGTGCTC TCTACATGGC ATGGTTAGAA 
GCTCTATCCA AGGACCTGCC ACCAATTCTC CTAGTCCGTG GGAATCATCA GAGTGTCCTT 
ACCTTCTATT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A2 ( XM_005209087.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209144.1 Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa21260
Clone ID Related Accession (Same CDS sequence)	XM_005209087.2
Accession Version	XM_005209087.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104020.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005209087.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG GGCCCACGGC GCCCTCCTCC GGCGCCCCGA GGCTGGCCGG GGTTGGGGAG 
ACGCCTCCAG TCGCCCCGCT GGCCGCCGCC GGGATGGACC TGCCCGGCAC AGCCGTGCCC 
TCAGTGCCGG AGGATGCTGC GGCTGTGAGC CGTGGCGGCG GTGGGGTCCG CGGGGAGGGC 
GCCGCGGCGG CCGGCGACGG ACTGGGCAGA CCCCTGGGGC CCACCCCGAG TCAGAGCCGC 
TTCCAGGTGG ATCTGGTTTC CGAGAATGCC GGGCGGGCGG CGGCGGCGGC GGCTAGCGCT 
GGGGCGGGAG GCAAGGAGAC CCCCGCGGAT GGGAAAGCCA GCGTCGAAAG CGGGCAGGCT 
AAGGCCAGCG AGGAAGCCAA GGGCCGCTTC CGCGTGAACT TCGTGGACCC GGCTGCCTCT 
TCGTCGGCGG ACGAGAGCGT GTCGGATGCG GCAGGGATCG GAGGCGACGG GCCCAACGTG 
AGCTTCCAGA ACGGCGGGGA CACGGTGCTG AGCGAGGGGA GCAGCCTGCA CTCGGGCGGC 
GGCGGCGGCG GCAGTGGGCA CCACCAGCAC TACTACTATG ACACCCACAC CAACACCTAC 
TACCTGCGCA CCTTCGGCCA CAACACTATG GACGCCGTGC CCAGGATCGA CCACTATCGG 
CACACGGCAG CGCAGCTAGG CGAGAAGCTG CTCCGGCCCA GCCTGGCGGA ACTACACGAT 
GAGTTGGAAA AGGAACCTTT TGAGGATGGC TTTGCAAATG GAGAAGAAAG CACGCCAACC 
AGAGAGGCTG TGGTCACATA CACTGCGGAA AGTAAAGGAG TCGTGAAGTT TGGCTGGATT 
AAGGGTGTAC TAGTACGTTG TATGTTAAAC ATTTGGGGAG TGATGCTCTT TATTAGATTG 
TCATGGATTG TGGGTCAAGC TGGAATAGGT CTGTCAGTCA TTGTAATAGC AATGGCCACT 
GTCGTGACAA CTATCACAGG ATTGTCTACA TCAGCAATAG CTACTAATGG GTTTGTACGA 
GGAGGAGGAG CGTATTATTT AATATCTAGA AGTCTAGGGC CAGAATTTGG TGGTGCAATT 
GGCCTGATCT TCGCTTTTGC CAATGCCGTT GCAGTTGCTA TGTACGTGGT TGGATTTGCA 
GAAACTGTGG TGGAGTTGCT TAAGGAACAT TCCATACTTA TGATAGATGA AATCAATGAT 
ATCCGAATTA TTGGAGCCAT TACAGTGGTG ATCCTTTTGG GTATCTCAGT AGCCGGAATG 
GAGTGGGAGG CAAAAGCTCA GATTGTTCTT TTGGTGATTC TACTACTTGC TATAGCTGAT 
TTCGTCATAG GAACATTTAT CCCATTGGAG AGCAAGAAGC CCAAAGGTTT CTTTGGTTAT 
AAGTATGAAA TATTTAGTGA GAACTTTGGG CCAGATTTTC GAGAAGAAGA GACCTTTTTT 
TCTGTATTTG CCATCTTTTT TCCTGCTGCA ACTGGCATTC TGGCTGGAGC AAATATCTCA 
GGTGATCTTG CAGATCCTCA GTCAGCCATA CCCAAAGGAA CACTCTTAGC CATTTTAATC 
ACTACGCTGG TTTACATAGG AATTGCAGTA TCTGTAGGTT CGTGTGTTGT TCGGGACGCT 
ACTGGGAACG TTAATGACAC CATTGTAACA GAGCTGACAA ACTGCACTTC TGCAGCCTGC 
AAGTTAAACT TTGATTTTTC ATCCTGTGAA ACCAACCCTT GTTCCTATGG CCTAATGAAC 
AACTTCCAGG TAATGAGCAT GGTGTCAGGC TTTGCACCAT TAATTTCTGC AGGTATCTTC 
TCAGCCACTC TTTCTTCTGC CTTAGCATCT CTTGTGAGTG CACCCAAAAT ATTTCAGGCT 
CTATGTAAGG ACAACATCTA CCCAGCTTTC CAGATGTTTG CTAAAGGTTA TGGGAAAAAT 
AATGAACCTC TTCGTGGCTA CATCTTAACA TTTTTAATTG CACTTGGATT CATCTTAATT 
GCTGAATTGA ATGTTATTGC TCCAATTATC TCTAACTTCT TCCTTGCATC ATATGCACTG 
ATCAATTTTT CAGTATTTCA TGCATCACTT GCAAAATCTC CAGGGTGGCG TCCTGCATTC 
AAATACTACA ATATGTGGAT ATCACTTCTT GGAGCAATTC TCTGTTGCAT AGTGATGTTT 
GTCATTAACT GGTGGGCTGC ATTGCTAACA TATGTGATAG TTCTTGGACT TTACATTTAT 
GTTACCTACA AAAAACCAGA TGTGAACTGG GGATCCTCCA CACAAGCTCT GACCTACCTG 
AATGCACTGC AGCATTCGAT TCGTCTTTCT GGAGTGGAAG ACCACGTGAA AAACTTCAGG 
CCTCAGTGTC TTGTTATGAC AGGTGCGCCA AACTCACGCC CAGCTTTACT TCATCTTGTT 
CACGATTTCA CAAAAAATGT TGGCTTGATG ATCTGTGGTC ATGTGCATAT GGGCCCTCGA 
AGACAAGCTA TGAAAGAGAT GTCCATTGAT CAAGCCAAAT ATCAGCGGTG GCTTATTAAA 
AACAAAATGA AGGCTTTTTA TGCTCCAGTA CATGCAGATG ATTTGAGAGA GGGTGCACAA 
TATTTGATGC AGGCTGCTGG CCTTGGGCGT ATGAAACCAA ACACACTTGT CCTTGGATTT 
AAGAAAGACT GGTTGCAAGC AGATATGAGG GATGTAGATA TGTATATAAA CTTATTTCAT 
GATGCTTTTG ATATACAATA TGGAGTAGTA GTTATCCGCC TACAAGAAGG TCTGGATATA 
TCTCATCTTC AAGGACAAGA AGAATTACTA TCATCGCAAG AGAAATCTCC TGGTGTAAAG 
GATGTGGTAC TAAGTGTGGA GTATAGTAAA AAATCAGATT TAGATACTTC TAAACCATCC 
AGTGAGAAAT CCATTACACA TAAAGAATCC AAAGGCCCTG CTGTGCCTTT AAACGTAGCT 
GACCAAAAGC TTCTTGAAGC TAGTACACAA TTTCAGAAAA AACAGGGAAA GAATACTATT 
GACGTCTGGT GGCTTTTTGA TGATGGAGGT TTGACTTTAT TGATACCTTA TCTTCTGACT 
ACCAAGAAAA AGTGGAAAGA TTGTAAGATC AGAGTATTCA TTGGTGGAAA AATAAATAGA 
ATAGACCATG ACCGAAGAGC GATGGCTACT TTGCTTAGCA AGTTCCGGAT AGACTTCTCT 
GATATCATGG TCTTAGGAGA TATTAATACT AAACCAAAGA AAGAAAATAT TGTAGCTTTT 
GATGAAATGA TTGAGCCATA CAGACTTCAT GAAGATGATA AAGAACAAGA TATTGCAGAT 
AAAATGAAAG AAGATGAACC ATGGCGAATA ACAGATAATG AGCTTGAACT TTATAAGACC 
AAGACATACC GGCAGATCAG GTTAAATGAA TTATTAAAGG AACATTCAAA CACAGCTAAT 
ATCATTGTCA TGAGTCTCCC AGTTGCACGA AAAGGTGCTG TGTCTAGTGC TCTCTACATG 
GCATGGTTAG AAGCTCTATC CAAGGACCTG CCACCAATTC TCCTAGTCCG TGGGAATCAT 
CAGAGTGTCC TTACCTTCTA TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209144.1
CDS	480..4046
Translation	MEPGPTAPSSGAPRLAGVGETPPVAPLAAAGMDLPGTAVPSVPEDAAAVSRGGGGVRGEGAAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAASAGAGGKETPADGKASVESGQAKASEEAKGRFRVNFVDPAASSSADESVSDAAGIGGDGPNVSFQNGGDTVLSEGSSLHSGGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTREAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVIVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFIPLESKKPKGFFGYKYEIFSENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYIGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCETNPCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLQEGLDISHLQGQEELLSSQEKSPGVKDVVLSVEYSKKSDLDTSKPSSEKSITHKESKGPAVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIVAFDEMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSNTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	XM_005209087.2
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209087.2

ORF Insert Sequence:

ATGGAGCCGG GGCCCACGGC GCCCTCCTCC GGCGCCCCGA GGCTGGCCGG GGTTGGGGAG 
ACGCCTCCAG TCGCCCCGCT GGCCGCCGCC GGGATGGACC TGCCCGGCAC AGCCGTGCCC 
TCAGTGCCGG AGGATGCTGC GGCTGTGAGC CGTGGCGGCG GTGGGGTCCG CGGGGAGGGC 
GCCGCGGCGG CCGGCGACGG ACTGGGCAGA CCCCTGGGGC CCACCCCGAG TCAGAGCCGC 
TTCCAGGTGG ATCTGGTTTC CGAGAATGCC GGGCGGGCGG CGGCGGCGGC GGCTAGCGCT 
GGGGCGGGAG GCAAGGAGAC CCCCGCGGAT GGGAAAGCCA GCGTCGAAAG CGGGCAGGCT 
AAGGCCAGCG AGGAAGCCAA GGGCCGCTTC CGCGTGAACT TCGTGGACCC GGCTGCCTCT 
TCGTCGGCGG ACGAGAGCGT GTCGGATGCG GCAGGGATCG GAGGCGACGG GCCCAACGTG 
AGCTTCCAGA ACGGCGGGGA CACGGTGCTG AGCGAGGGGA GCAGCCTGCA CTCGGGCGGC 
GGCGGCGGCG GCAGTGGGCA CCACCAGCAC TACTACTATG ACACCCACAC CAACACCTAC 
TACCTGCGCA CCTTCGGCCA CAACACTATG GACGCCGTGC CCAGGATCGA CCACTATCGG 
CACACGGCAG CGCAGCTAGG CGAGAAGCTG CTCCGGCCCA GCCTGGCGGA ACTACACGAT 
GAGTTGGAAA AGGAACCTTT TGAGGATGGC TTTGCAAATG GAGAAGAAAG CACGCCAACC 
AGAGAGGCTG TGGTCACATA CACTGCGGAA AGTAAAGGAG TCGTGAAGTT TGGCTGGATT 
AAGGGTGTAC TAGTACGTTG TATGTTAAAC ATTTGGGGAG TGATGCTCTT TATTAGATTG 
TCATGGATTG TGGGTCAAGC TGGAATAGGT CTGTCAGTCA TTGTAATAGC AATGGCCACT 
GTCGTGACAA CTATCACAGG ATTGTCTACA TCAGCAATAG CTACTAATGG GTTTGTACGA 
GGAGGAGGAG CGTATTATTT AATATCTAGA AGTCTAGGGC CAGAATTTGG TGGTGCAATT 
GGCCTGATCT TCGCTTTTGC CAATGCCGTT GCAGTTGCTA TGTACGTGGT TGGATTTGCA 
GAAACTGTGG TGGAGTTGCT TAAGGAACAT TCCATACTTA TGATAGATGA AATCAATGAT 
ATCCGAATTA TTGGAGCCAT TACAGTGGTG ATCCTTTTGG GTATCTCAGT AGCCGGAATG 
GAGTGGGAGG CAAAAGCTCA GATTGTTCTT TTGGTGATTC TACTACTTGC TATAGCTGAT 
TTCGTCATAG GAACATTTAT CCCATTGGAG AGCAAGAAGC CCAAAGGTTT CTTTGGTTAT 
AAGTATGAAA TATTTAGTGA GAACTTTGGG CCAGATTTTC GAGAAGAAGA GACCTTTTTT 
TCTGTATTTG CCATCTTTTT TCCTGCTGCA ACTGGCATTC TGGCTGGAGC AAATATCTCA 
GGTGATCTTG CAGATCCTCA GTCAGCCATA CCCAAAGGAA CACTCTTAGC CATTTTAATC 
ACTACGCTGG TTTACATAGG AATTGCAGTA TCTGTAGGTT CGTGTGTTGT TCGGGACGCT 
ACTGGGAACG TTAATGACAC CATTGTAACA GAGCTGACAA ACTGCACTTC TGCAGCCTGC 
AAGTTAAACT TTGATTTTTC ATCCTGTGAA ACCAACCCTT GTTCCTATGG CCTAATGAAC 
AACTTCCAGG TAATGAGCAT GGTGTCAGGC TTTGCACCAT TAATTTCTGC AGGTATCTTC 
TCAGCCACTC TTTCTTCTGC CTTAGCATCT CTTGTGAGTG CACCCAAAAT ATTTCAGGCT 
CTATGTAAGG ACAACATCTA CCCAGCTTTC CAGATGTTTG CTAAAGGTTA TGGGAAAAAT 
AATGAACCTC TTCGTGGCTA CATCTTAACA TTTTTAATTG CACTTGGATT CATCTTAATT 
GCTGAATTGA ATGTTATTGC TCCAATTATC TCTAACTTCT TCCTTGCATC ATATGCACTG 
ATCAATTTTT CAGTATTTCA TGCATCACTT GCAAAATCTC CAGGGTGGCG TCCTGCATTC 
AAATACTACA ATATGTGGAT ATCACTTCTT GGAGCAATTC TCTGTTGCAT AGTGATGTTT 
GTCATTAACT GGTGGGCTGC ATTGCTAACA TATGTGATAG TTCTTGGACT TTACATTTAT 
GTTACCTACA AAAAACCAGA TGTGAACTGG GGATCCTCCA CACAAGCTCT GACCTACCTG 
AATGCACTGC AGCATTCGAT TCGTCTTTCT GGAGTGGAAG ACCACGTGAA AAACTTCAGG 
CCTCAGTGTC TTGTTATGAC AGGTGCGCCA AACTCACGCC CAGCTTTACT TCATCTTGTT 
CACGATTTCA CAAAAAATGT TGGCTTGATG ATCTGTGGTC ATGTGCATAT GGGCCCTCGA 
AGACAAGCTA TGAAAGAGAT GTCCATTGAT CAAGCCAAAT ATCAGCGGTG GCTTATTAAA 
AACAAAATGA AGGCTTTTTA TGCTCCAGTA CATGCAGATG ATTTGAGAGA GGGTGCACAA 
TATTTGATGC AGGCTGCTGG CCTTGGGCGT ATGAAACCAA ACACACTTGT CCTTGGATTT 
AAGAAAGACT GGTTGCAAGC AGATATGAGG GATGTAGATA TGTATATAAA CTTATTTCAT 
GATGCTTTTG ATATACAATA TGGAGTAGTA GTTATCCGCC TACAAGAAGG TCTGGATATA 
TCTCATCTTC AAGGACAAGA AGAATTACTA TCATCGCAAG AGAAATCTCC TGGTGTAAAG 
GATGTGGTAC TAAGTGTGGA GTATAGTAAA AAATCAGATT TAGATACTTC TAAACCATCC 
AGTGAGAAAT CCATTACACA TAAAGAATCC AAAGGCCCTG CTGTGCCTTT AAACGTAGCT 
GACCAAAAGC TTCTTGAAGC TAGTACACAA TTTCAGAAAA AACAGGGAAA GAATACTATT 
GACGTCTGGT GGCTTTTTGA TGATGGAGGT TTGACTTTAT TGATACCTTA TCTTCTGACT 
ACCAAGAAAA AGTGGAAAGA TTGTAAGATC AGAGTATTCA TTGGTGGAAA AATAAATAGA 
ATAGACCATG ACCGAAGAGC GATGGCTACT TTGCTTAGCA AGTTCCGGAT AGACTTCTCT 
GATATCATGG TCTTAGGAGA TATTAATACT AAACCAAAGA AAGAAAATAT TGTAGCTTTT 
GATGAAATGA TTGAGCCATA CAGACTTCAT GAAGATGATA AAGAACAAGA TATTGCAGAT 
AAAATGAAAG AAGATGAACC ATGGCGAATA ACAGATAATG AGCTTGAACT TTATAAGACC 
AAGACATACC GGCAGATCAG GTTAAATGAA TTATTAAAGG AACATTCAAA CACAGCTAAT 
ATCATTGTCA TGAGTCTCCC AGTTGCACGA AAAGGTGCTG TGTCTAGTGC TCTCTACATG 
GCATGGTTAG AAGCTCTATC CAAGGACCTG CCACCAATTC TCCTAGTCCG TGGGAATCAT 
CAGAGTGTCC TTACCTTCTA TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Molecular characterization of the Na-K-Cl cotransporter of bovine aortic endothelial cells.
The American journal of physiology273(1 Pt 1)C188-97(1997 Jul)
Yerby TR,Vibat CR,Sun D,Payne JA,O'Donnell ME

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SLC12A2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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