GAREM1 cDNA ORF clone, Homo sapiens(human)

The following GAREM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAREM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu21694 NM_001242409.2
Latest version!
Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu00218 NM_022751.3
Latest version!
Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
OHu71459 XM_017025919.1
Latest version!
Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu104569 XM_024451235.1
Latest version!
Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu104569 XM_024451234.1
Latest version!
Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu104570 XM_024451236.1
Latest version!
Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu21694 NM_001242409.1 Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu00218 NM_022751.2 Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu21694
    Clone ID Related Accession (Same CDS sequence) NM_001242409.1 , NM_001242409.2
    Accession Version NM_001242409.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2631bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product GRB2-associated and regulator of MAPK protein 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK025263.1, BC121068.1, AK025660.1, AC015563.11 and AI479499.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.258763.1, SRR1803617.223086.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467150 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
    GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC
    GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG
    TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA
    GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG
    GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG
    TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC
    ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA
    GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG
    ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT
    ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC
    ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG
    GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC
    GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG
    GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC
    TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA
    ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA
    CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT
    CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC
    CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG
    GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC
    CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA
    GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA
    GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG
    TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG
    GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT
    GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA
    AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG
    ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCAGCGTC
    ACTAAAACTG ACACAAATCC TTCTGAAAGC ACTCCTGTTT CCTGCTATCC ATGTAACCGA
    GTGAAAACTG ATTCTGTGGA CCTGAAATCC CCGTTTGGAA GTCCTTCTGC TGAAGCTGTG
    TCCTCTCGGC TCTCATGGCC TAACCATTAT TCAGGAGCAT CAGAAAGCCA GACCAGGAGT
    GACTTCCTGC TGGATCCAAG CAGGAGTTAT AGTTACCCTA GACAAAAGAC GCCAGGCACA
    CCAAAGAGAA ACTGCCCAGC ACCTTTTGAT TTTGATGGCT GTGAGCTCCT GGCCAGCCCC
    ACTAGCCCAG TCACTGCAGA ATTCAGTAGC AGCGTCTCTG GTTGTCCCAA GTCAGCCAGC
    TACTCTCTGG AGAGCACAGA TGTGAAATCT CTTGCAGCTG GTGTGACAAA GCAGAGTACG
    TCATGCCCTG CCTTACCCCC CAGGGCTCCA AAACTAGTGG AAGAGAAGGT CGCCTCCGAA
    ACATCTCCTT TGCCTCTGAA AATTGATGGT GCTGAGGAAG ACCCCAAGTC TGGGTCACCA
    GATCTCTCGG AGGACCAGTA TTTTGTTAAA AAGGGCATGC AGGACATCTT CTCTGCCTCC
    TACCCTTTCT CATCTCCGCT CCATCTCCAG CTGGCCCCCA GATCCTGTGG CGACGGTTCC
    CCATGGCAGC CACCTGCTGA CCTATCAGGA CTCTCTATAG AGGAAGTGTC CAAGTCACTA
    CGGTTCATTG GTTTGTCCGA AGATGTCATA TCATTCTTTG TTACTGAAAA GATTGATGGG
    AACCTGCTTG TTCAGCTAAC GGAAGAAATC CTCTCAGAGG ATTTCAAATT GAGCAAATTG
    CAGGTGAAGA AGATAATGCA ATTCATTAAT GGCTGGAGGC CCAAAATATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001229338.1
    CDS57..2687
    Translation

    Target ORF information:

    RefSeq Version NM_001242409.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001242409.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
    GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC
    GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG
    TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA
    GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG
    GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG
    TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC
    ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA
    GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG
    ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT
    ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC
    ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG
    GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC
    GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG
    GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC
    TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA
    ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA
    CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT
    CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC
    CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG
    GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC
    CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA
    GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA
    GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG
    TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG
    GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT
    GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA
    AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG
    ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCAGCGTC
    ACTAAAACTG ACACAAATCC TTCTGAAAGC ACTCCTGTTT CCTGCTATCC ATGTAACCGA
    GTGAAAACTG ATTCTGTGGA CCTGAAATCC CCGTTTGGAA GTCCTTCTGC TGAAGCTGTG
    TCCTCTCGGC TCTCATGGCC TAACCATTAT TCAGGAGCAT CAGAAAGCCA GACCAGGAGT
    GACTTCCTGC TGGATCCAAG CAGGAGTTAT AGTTACCCTA GACAAAAGAC GCCAGGCACA
    CCAAAGAGAA ACTGCCCAGC ACCTTTTGAT TTTGATGGCT GTGAGCTCCT GGCCAGCCCC
    ACTAGCCCAG TCACTGCAGA ATTCAGTAGC AGCGTCTCTG GTTGTCCCAA GTCAGCCAGC
    TACTCTCTGG AGAGCACAGA TGTGAAATCT CTTGCAGCTG GTGTGACAAA GCAGAGTACG
    TCATGCCCTG CCTTACCCCC CAGGGCTCCA AAACTAGTGG AAGAGAAGGT CGCCTCCGAA
    ACATCTCCTT TGCCTCTGAA AATTGATGGT GCTGAGGAAG ACCCCAAGTC TGGGTCACCA
    GATCTCTCGG AGGACCAGTA TTTTGTTAAA AAGGGCATGC AGGACATCTT CTCTGCCTCC
    TACCCTTTCT CATCTCCGCT CCATCTCCAG CTGGCCCCCA GATCCTGTGG CGACGGTTCC
    CCATGGCAGC CACCTGCTGA CCTATCAGGA CTCTCTATAG AGGAAGTGTC CAAGTCACTA
    CGGTTCATTG GTTTGTCCGA AGATGTCATA TCATTCTTTG TTACTGAAAA GATTGATGGG
    AACCTGCTTG TTCAGCTAAC GGAAGAAATC CTCTCAGAGG ATTTCAAATT GAGCAAATTG
    CAGGTGAAGA AGATAATGCA ATTCATTAAT GGCTGGAGGC CCAAAATATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21694
    Clone ID Related Accession (Same CDS sequence) NM_001242409.1 , NM_001242409.2
    Accession Version NM_001242409.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2631bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product GRB2-associated and regulator of MAPK protein 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009835.14, AK025263.1, BC121068.1, AK025660.1, AC015563.11 and AI479499.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001242409.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.258763.1, SRR1803617.223086.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467150 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000269209.7/ ENSP00000269209.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
    GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC
    GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG
    TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA
    GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG
    GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG
    TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC
    ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA
    GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG
    ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT
    ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC
    ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG
    GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC
    GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG
    GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC
    TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA
    ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA
    CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT
    CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC
    CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG
    GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC
    CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA
    GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA
    GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG
    TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG
    GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT
    GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA
    AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG
    ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCAGCGTC
    ACTAAAACTG ACACAAATCC TTCTGAAAGC ACTCCTGTTT CCTGCTATCC ATGTAACCGA
    GTGAAAACTG ATTCTGTGGA CCTGAAATCC CCGTTTGGAA GTCCTTCTGC TGAAGCTGTG
    TCCTCTCGGC TCTCATGGCC TAACCATTAT TCAGGAGCAT CAGAAAGCCA GACCAGGAGT
    GACTTCCTGC TGGATCCAAG CAGGAGTTAT AGTTACCCTA GACAAAAGAC GCCAGGCACA
    CCAAAGAGAA ACTGCCCAGC ACCTTTTGAT TTTGATGGCT GTGAGCTCCT GGCCAGCCCC
    ACTAGCCCAG TCACTGCAGA ATTCAGTAGC AGCGTCTCTG GTTGTCCCAA GTCAGCCAGC
    TACTCTCTGG AGAGCACAGA TGTGAAATCT CTTGCAGCTG GTGTGACAAA GCAGAGTACG
    TCATGCCCTG CCTTACCCCC CAGGGCTCCA AAACTAGTGG AAGAGAAGGT CGCCTCCGAA
    ACATCTCCTT TGCCTCTGAA AATTGATGGT GCTGAGGAAG ACCCCAAGTC TGGGTCACCA
    GATCTCTCGG AGGACCAGTA TTTTGTTAAA AAGGGCATGC AGGACATCTT CTCTGCCTCC
    TACCCTTTCT CATCTCCGCT CCATCTCCAG CTGGCCCCCA GATCCTGTGG CGACGGTTCC
    CCATGGCAGC CACCTGCTGA CCTATCAGGA CTCTCTATAG AGGAAGTGTC CAAGTCACTA
    CGGTTCATTG GTTTGTCCGA AGATGTCATA TCATTCTTTG TTACTGAAAA GATTGATGGG
    AACCTGCTTG TTCAGCTAAC GGAAGAAATC CTCTCAGAGG ATTTCAAATT GAGCAAATTG
    CAGGTGAAGA AGATAATGCA ATTCATTAAT GGCTGGAGGC CCAAAATATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001229338.1
    CDS455..3085
    Translation

    Target ORF information:

    RefSeq Version NM_001242409.2
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001242409.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
    GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC
    GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG
    TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA
    GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG
    GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG
    TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC
    ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA
    GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG
    ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT
    ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC
    ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG
    GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC
    GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG
    GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC
    TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA
    ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA
    CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT
    CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC
    CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG
    GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC
    CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA
    GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA
    GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG
    TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG
    GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT
    GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA
    AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG
    ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCAGCGTC
    ACTAAAACTG ACACAAATCC TTCTGAAAGC ACTCCTGTTT CCTGCTATCC ATGTAACCGA
    GTGAAAACTG ATTCTGTGGA CCTGAAATCC CCGTTTGGAA GTCCTTCTGC TGAAGCTGTG
    TCCTCTCGGC TCTCATGGCC TAACCATTAT TCAGGAGCAT CAGAAAGCCA GACCAGGAGT
    GACTTCCTGC TGGATCCAAG CAGGAGTTAT AGTTACCCTA GACAAAAGAC GCCAGGCACA
    CCAAAGAGAA ACTGCCCAGC ACCTTTTGAT TTTGATGGCT GTGAGCTCCT GGCCAGCCCC
    ACTAGCCCAG TCACTGCAGA ATTCAGTAGC AGCGTCTCTG GTTGTCCCAA GTCAGCCAGC
    TACTCTCTGG AGAGCACAGA TGTGAAATCT CTTGCAGCTG GTGTGACAAA GCAGAGTACG
    TCATGCCCTG CCTTACCCCC CAGGGCTCCA AAACTAGTGG AAGAGAAGGT CGCCTCCGAA
    ACATCTCCTT TGCCTCTGAA AATTGATGGT GCTGAGGAAG ACCCCAAGTC TGGGTCACCA
    GATCTCTCGG AGGACCAGTA TTTTGTTAAA AAGGGCATGC AGGACATCTT CTCTGCCTCC
    TACCCTTTCT CATCTCCGCT CCATCTCCAG CTGGCCCCCA GATCCTGTGG CGACGGTTCC
    CCATGGCAGC CACCTGCTGA CCTATCAGGA CTCTCTATAG AGGAAGTGTC CAAGTCACTA
    CGGTTCATTG GTTTGTCCGA AGATGTCATA TCATTCTTTG TTACTGAAAA GATTGATGGG
    AACCTGCTTG TTCAGCTAAC GGAAGAAATC CTCTCAGAGG ATTTCAAATT GAGCAAATTG
    CAGGTGAAGA AGATAATGCA ATTCATTAAT GGCTGGAGGC CCAAAATATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu00218
    Clone ID Related Accession (Same CDS sequence) NM_022751.2 , NM_022751.3
    Accession Version NM_022751.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product GRB2-associated and regulator of MAPK protein 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK025263.1, AK025660.1, AC015563.11 and AI479499.1. On Jun 3, 2011 this sequence version replaced NM_022751.1. Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at the 3' terminal exon compared to variant 1. This results in an isoform (2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK025263.1, SRR1803617.91166.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
    GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC
    GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG
    TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA
    GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG
    GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG
    TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC
    ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA
    GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG
    ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT
    ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC
    ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG
    GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC
    GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG
    GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC
    TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA
    ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA
    CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT
    CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC
    CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG
    GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC
    CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA
    GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA
    GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG
    TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG
    GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT
    GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA
    AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG
    ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCGTCACT
    AAAACTGACA CAAATCCTTC TGAAAGCACT CCTGTTTCCT GCTATCCATG TAACCGAGTG
    AAAACTGATT CTGTGGACCT GAAATCCCCG TTTGGAAGTC CTTCTGCTGA AGCTGTGTCC
    TCTCGGCTCT CATGGCCTAA CCATTATTCA GGAGCATCAG AAAGCCAGAC CAGGAGTGAC
    TTCCTGCTGG ATCCAAGCAG GAGTTATAGT TACCCTAGAC AAAAGACGCC AGGCACACCA
    AAGAGAAACT GCCCAGCACC TTTTGATTTT GATGGCTGTG AGCTCCTGGC CAGCCCCACT
    AGCCCAGTCA CTGCAGAATT CAGTAGCAGC GTCTCTGGTT GTCCCAAGTC AGCCAGCTAC
    TCTCTGGAGA GCACAGATGT GAAATCTCTT GCAGCTGGTG TGACAAAGCA GAGTACGTCA
    TGCCCTGCCT TACCCCCCAG GGCTCCAAAA CTAGTGGAAG AGAAGGTCGC CTCCGAAACA
    TCTCCTTTGC CTCTGAAAAT TGATGGTGCT GAGGAAGACC CCAAGTCTGG GTCACCAGAT
    CTCTCGGAGG ACCAGTATTT TGTTAAAAAG GGCATGCAGG ACATCTTCTC TGCCTCCTAC
    CCTTTCTCAT CTCCGCTCCA TCTCCAGCTG GCCCCCAGAT CCTGTGGCGA CGGTTCCCCA
    TGGCAGCCAC CTGCTGACCT ATCAGGACTC TCTATAGAGG AAGTGTCCAA GTCACTACGG
    TTCATTGGTT TGTCCGAAGA TGTCATATCA TTCTTTGTTA CTGAAAAGAT TGATGGGAAC
    CTGCTTGTTC AGCTAACGGA AGAAATCCTC TCAGAGGATT TCAAATTGAG CAAATTGCAG
    GTGAAGAAGA TAATGCAATT CATTAATGGC TGGAGGCCCA AAATATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_073588.1
    CDS57..2684
    Translation

    Target ORF information:

    RefSeq Version NM_022751.2
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_022751.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
    GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC
    GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG
    TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA
    GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG
    GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG
    TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC
    ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA
    GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG
    ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT
    ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC
    ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG
    GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC
    GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG
    GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC
    TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA
    ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA
    CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT
    CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC
    CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG
    GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC
    CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA
    GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA
    GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG
    TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG
    GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT
    GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA
    AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG
    ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCGTCACT
    AAAACTGACA CAAATCCTTC TGAAAGCACT CCTGTTTCCT GCTATCCATG TAACCGAGTG
    AAAACTGATT CTGTGGACCT GAAATCCCCG TTTGGAAGTC CTTCTGCTGA AGCTGTGTCC
    TCTCGGCTCT CATGGCCTAA CCATTATTCA GGAGCATCAG AAAGCCAGAC CAGGAGTGAC
    TTCCTGCTGG ATCCAAGCAG GAGTTATAGT TACCCTAGAC AAAAGACGCC AGGCACACCA
    AAGAGAAACT GCCCAGCACC TTTTGATTTT GATGGCTGTG AGCTCCTGGC CAGCCCCACT
    AGCCCAGTCA CTGCAGAATT CAGTAGCAGC GTCTCTGGTT GTCCCAAGTC AGCCAGCTAC
    TCTCTGGAGA GCACAGATGT GAAATCTCTT GCAGCTGGTG TGACAAAGCA GAGTACGTCA
    TGCCCTGCCT TACCCCCCAG GGCTCCAAAA CTAGTGGAAG AGAAGGTCGC CTCCGAAACA
    TCTCCTTTGC CTCTGAAAAT TGATGGTGCT GAGGAAGACC CCAAGTCTGG GTCACCAGAT
    CTCTCGGAGG ACCAGTATTT TGTTAAAAAG GGCATGCAGG ACATCTTCTC TGCCTCCTAC
    CCTTTCTCAT CTCCGCTCCA TCTCCAGCTG GCCCCCAGAT CCTGTGGCGA CGGTTCCCCA
    TGGCAGCCAC CTGCTGACCT ATCAGGACTC TCTATAGAGG AAGTGTCCAA GTCACTACGG
    TTCATTGGTT TGTCCGAAGA TGTCATATCA TTCTTTGTTA CTGAAAAGAT TGATGGGAAC
    CTGCTTGTTC AGCTAACGGA AGAAATCCTC TCAGAGGATT TCAAATTGAG CAAATTGCAG
    GTGAAGAAGA TAATGCAATT CATTAATGGC TGGAGGCCCA AAATATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu00218
    Clone ID Related Accession (Same CDS sequence) NM_022751.2 , NM_022751.3
    Accession Version NM_022751.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product GRB2-associated and regulator of MAPK protein 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009835.14, AK025263.1, AK025660.1, AC015563.11 and AI479499.1. On May 31, 2019 this sequence version replaced NM_022751.2. Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at the 3' terminal exon compared to variant 1. This results in an isoform (2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.91166.1, AK025263.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
    GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC
    GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG
    TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA
    GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG
    GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG
    TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC
    ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA
    GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG
    ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT
    ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC
    ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG
    GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC
    GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG
    GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC
    TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA
    ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA
    CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT
    CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC
    CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG
    GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC
    CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA
    GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA
    GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG
    TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG
    GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT
    GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA
    AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG
    ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCGTCACT
    AAAACTGACA CAAATCCTTC TGAAAGCACT CCTGTTTCCT GCTATCCATG TAACCGAGTG
    AAAACTGATT CTGTGGACCT GAAATCCCCG TTTGGAAGTC CTTCTGCTGA AGCTGTGTCC
    TCTCGGCTCT CATGGCCTAA CCATTATTCA GGAGCATCAG AAAGCCAGAC CAGGAGTGAC
    TTCCTGCTGG ATCCAAGCAG GAGTTATAGT TACCCTAGAC AAAAGACGCC AGGCACACCA
    AAGAGAAACT GCCCAGCACC TTTTGATTTT GATGGCTGTG AGCTCCTGGC CAGCCCCACT
    AGCCCAGTCA CTGCAGAATT CAGTAGCAGC GTCTCTGGTT GTCCCAAGTC AGCCAGCTAC
    TCTCTGGAGA GCACAGATGT GAAATCTCTT GCAGCTGGTG TGACAAAGCA GAGTACGTCA
    TGCCCTGCCT TACCCCCCAG GGCTCCAAAA CTAGTGGAAG AGAAGGTCGC CTCCGAAACA
    TCTCCTTTGC CTCTGAAAAT TGATGGTGCT GAGGAAGACC CCAAGTCTGG GTCACCAGAT
    CTCTCGGAGG ACCAGTATTT TGTTAAAAAG GGCATGCAGG ACATCTTCTC TGCCTCCTAC
    CCTTTCTCAT CTCCGCTCCA TCTCCAGCTG GCCCCCAGAT CCTGTGGCGA CGGTTCCCCA
    TGGCAGCCAC CTGCTGACCT ATCAGGACTC TCTATAGAGG AAGTGTCCAA GTCACTACGG
    TTCATTGGTT TGTCCGAAGA TGTCATATCA TTCTTTGTTA CTGAAAAGAT TGATGGGAAC
    CTGCTTGTTC AGCTAACGGA AGAAATCCTC TCAGAGGATT TCAAATTGAG CAAATTGCAG
    GTGAAGAAGA TAATGCAATT CATTAATGGC TGGAGGCCCA AAATATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_073588.1
    CDS455..3082
    Translation

    Target ORF information:

    RefSeq Version NM_022751.3
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_022751.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
    GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC
    GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG
    TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA
    GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG
    GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG
    TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC
    ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA
    GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG
    ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT
    ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC
    ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG
    GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC
    GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG
    GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC
    TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA
    ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA
    CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT
    CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC
    CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG
    GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC
    CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA
    GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA
    GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG
    TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG
    GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT
    GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA
    AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG
    ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCGTCACT
    AAAACTGACA CAAATCCTTC TGAAAGCACT CCTGTTTCCT GCTATCCATG TAACCGAGTG
    AAAACTGATT CTGTGGACCT GAAATCCCCG TTTGGAAGTC CTTCTGCTGA AGCTGTGTCC
    TCTCGGCTCT CATGGCCTAA CCATTATTCA GGAGCATCAG AAAGCCAGAC CAGGAGTGAC
    TTCCTGCTGG ATCCAAGCAG GAGTTATAGT TACCCTAGAC AAAAGACGCC AGGCACACCA
    AAGAGAAACT GCCCAGCACC TTTTGATTTT GATGGCTGTG AGCTCCTGGC CAGCCCCACT
    AGCCCAGTCA CTGCAGAATT CAGTAGCAGC GTCTCTGGTT GTCCCAAGTC AGCCAGCTAC
    TCTCTGGAGA GCACAGATGT GAAATCTCTT GCAGCTGGTG TGACAAAGCA GAGTACGTCA
    TGCCCTGCCT TACCCCCCAG GGCTCCAAAA CTAGTGGAAG AGAAGGTCGC CTCCGAAACA
    TCTCCTTTGC CTCTGAAAAT TGATGGTGCT GAGGAAGACC CCAAGTCTGG GTCACCAGAT
    CTCTCGGAGG ACCAGTATTT TGTTAAAAAG GGCATGCAGG ACATCTTCTC TGCCTCCTAC
    CCTTTCTCAT CTCCGCTCCA TCTCCAGCTG GCCCCCAGAT CCTGTGGCGA CGGTTCCCCA
    TGGCAGCCAC CTGCTGACCT ATCAGGACTC TCTATAGAGG AAGTGTCCAA GTCACTACGG
    TTCATTGGTT TGTCCGAAGA TGTCATATCA TTCTTTGTTA CTGAAAAGAT TGATGGGAAC
    CTGCTTGTTC AGCTAACGGA AGAAATCCTC TCAGAGGATT TCAAATTGAG CAAATTGCAG
    GTGAAGAAGA TAATGCAATT CATTAATGGC TGGAGGCCCA AAATATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71459
    Clone ID Related Accession (Same CDS sequence) XM_017025919.1
    Accession Version XM_017025919.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product GRB2-associated and regulator of MAPK protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
    GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC
    GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG
    TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA
    GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG
    GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG
    TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC
    ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA
    GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG
    ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT
    ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC
    ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG
    GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC
    GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG
    GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC
    TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA
    ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA
    CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT
    CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC
    CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG
    GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC
    CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA
    GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA
    GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG
    TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG
    GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT
    GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA
    AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG
    ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATAATGGAA
    GAGCCAATGT CTTGGGAAGA AAAGATGCCA CACCAGTCTG AGAAATTCAG AGGTAAAGGC
    CAACTAGATT CTCCTGGAAA CAACCCGGGG GCTGCCATCA GTTCTCACAA GTCAGCATCT
    GGTTCTTTCC ACTTTGACAC GAGCAGACAG CACCTTCAAG CAGACAACAC CTTCGACCAG
    GACCTAAAAT GGAAAGGGTC CCATTGGCTA TCTACTCTGA ATTTCCATCC AGACAGGAGT
    CCCCTTTTGA CCATCACTGT TGAGTGGTCA TTTCCTTTTG TCTGGATACT TCCAGTGCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016881408.1
    CDS91..2133
    Translation

    Target ORF information:

    RefSeq Version XM_017025919.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017025919.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
    GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC
    GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG
    TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA
    GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG
    GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG
    TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC
    ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA
    GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG
    ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT
    ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC
    ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG
    GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC
    GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG
    GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC
    TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA
    ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA
    CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT
    CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC
    CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG
    GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC
    CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA
    GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA
    GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG
    TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG
    GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT
    GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA
    AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG
    ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATAATGGAA
    GAGCCAATGT CTTGGGAAGA AAAGATGCCA CACCAGTCTG AGAAATTCAG AGGTAAAGGC
    CAACTAGATT CTCCTGGAAA CAACCCGGGG GCTGCCATCA GTTCTCACAA GTCAGCATCT
    GGTTCTTTCC ACTTTGACAC GAGCAGACAG CACCTTCAAG CAGACAACAC CTTCGACCAG
    GACCTAAAAT GGAAAGGGTC CCATTGGCTA TCTACTCTGA ATTTCCATCC AGACAGGAGT
    CCCCTTTTGA CCATCACTGT TGAGTGGTCA TTTCCTTTTG TCTGGATACT TCCAGTGCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu104569
    Clone ID Related Accession (Same CDS sequence) XM_024451234.1 , XM_024451235.1
    Accession Version XM_024451234.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product GRB2-associated and regulator of MAPK protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
    GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC
    CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG
    AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTCAT TTGTATGAAT
    CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA
    AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA
    ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC
    CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG
    GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT
    GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGAGCTGGCC CGAAACCCTG
    GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT
    GTCCGTGATG TGAAAACCGA CTGGAATGAA GAATGCAAGA GCCCCAAGAA GGGTCGGTGC
    TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC
    TTCCACCGAC TCTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC
    CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC
    TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA
    GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA GGAAGGCAAG
    CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGCGAGAAGA ACAGATGTGA TCAGTTTAGA
    GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA
    GCAGTGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC
    GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT
    TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC
    TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCAG CGTCACTAAA
    ACTGACACAA ATCCTTCTGA AAGCACTCCT GTTTCCTGCT ATCCATGTAA CCGAGTGAAA
    ACTGATTCTG TGGACCTGAA ATCCCCGTTT GGAAGTCCTT CTGCTGAAGC TGTGTCCTCT
    CGGCTCTCAT GGCCTAACCA TTATTCAGGA GCATCAGAAA GCCAGACCAG GAGTGACTTC
    CTGCTGGATC CAAGCAGGAG TTATAGTTAC CCTAGACAAA AGACGCCAGG CACACCAAAG
    AGAAACTGCC CAGCACCTTT TGATTTTGAT GGCTGTGAGC TCCTGGCCAG CCCCACTAGC
    CCAGTCACTG CAGAATTCAG TAGCAGCGTC TCTGGTTGTC CCAAGTCAGC CAGCTACTCT
    CTGGAGAGCA CAGATGTGAA ATCTCTTGCA GCTGGTGTGA CAAAGCAGAG TACGTCATGC
    CCTGCCTTAC CCCCCAGGGC TCCAAAACTA GTGGAAGAGA AGGTCGCCTC CGAAACATCT
    CCTTTGCCTC TGAAAATTGA TGGTGCTGAG GAAGACCCCA AGTCTGGGTC ACCAGATCTC
    TCGGAGGACC AGTATTTTGT TAAAAAGGGC ATGCAGGACA TCTTCTCTGC CTCCTACCCT
    TTCTCATCTC CGCTCCATCT CCAGCTGGCC CCCAGATCCT GTGGCGACGG TTCCCCATGG
    CAGCCACCTG CTGACCTATC AGGACTCTCT ATAGAGGAAG TGTCCAAGTC ACTACGGTTC
    ATTGGTTTGT CCGAAGATGT CATATCATTC TTTGTTACTG AAAAGATTGA TGGGAACCTG
    CTTGTTCAGC TAACGGAAGA AATCCTCTCA GAGGATTTCA AATTGAGCAA ATTGCAGGTG
    AAGAAGATAA TGCAATTCAT TAATGGCTGG AGGCCCAAAA TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024307002.1
    CDS387..2651
    Translation

    Target ORF information:

    RefSeq Version XM_024451234.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024451234.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
    GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC
    CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG
    AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTCAT TTGTATGAAT
    CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA
    AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA
    ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC
    CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG
    GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT
    GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGAGCTGGCC CGAAACCCTG
    GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT
    GTCCGTGATG TGAAAACCGA CTGGAATGAA GAATGCAAGA GCCCCAAGAA GGGTCGGTGC
    TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC
    TTCCACCGAC TCTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC
    CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC
    TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA
    GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA GGAAGGCAAG
    CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGCGAGAAGA ACAGATGTGA TCAGTTTAGA
    GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA
    GCAGTGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC
    GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT
    TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC
    TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCAG CGTCACTAAA
    ACTGACACAA ATCCTTCTGA AAGCACTCCT GTTTCCTGCT ATCCATGTAA CCGAGTGAAA
    ACTGATTCTG TGGACCTGAA ATCCCCGTTT GGAAGTCCTT CTGCTGAAGC TGTGTCCTCT
    CGGCTCTCAT GGCCTAACCA TTATTCAGGA GCATCAGAAA GCCAGACCAG GAGTGACTTC
    CTGCTGGATC CAAGCAGGAG TTATAGTTAC CCTAGACAAA AGACGCCAGG CACACCAAAG
    AGAAACTGCC CAGCACCTTT TGATTTTGAT GGCTGTGAGC TCCTGGCCAG CCCCACTAGC
    CCAGTCACTG CAGAATTCAG TAGCAGCGTC TCTGGTTGTC CCAAGTCAGC CAGCTACTCT
    CTGGAGAGCA CAGATGTGAA ATCTCTTGCA GCTGGTGTGA CAAAGCAGAG TACGTCATGC
    CCTGCCTTAC CCCCCAGGGC TCCAAAACTA GTGGAAGAGA AGGTCGCCTC CGAAACATCT
    CCTTTGCCTC TGAAAATTGA TGGTGCTGAG GAAGACCCCA AGTCTGGGTC ACCAGATCTC
    TCGGAGGACC AGTATTTTGT TAAAAAGGGC ATGCAGGACA TCTTCTCTGC CTCCTACCCT
    TTCTCATCTC CGCTCCATCT CCAGCTGGCC CCCAGATCCT GTGGCGACGG TTCCCCATGG
    CAGCCACCTG CTGACCTATC AGGACTCTCT ATAGAGGAAG TGTCCAAGTC ACTACGGTTC
    ATTGGTTTGT CCGAAGATGT CATATCATTC TTTGTTACTG AAAAGATTGA TGGGAACCTG
    CTTGTTCAGC TAACGGAAGA AATCCTCTCA GAGGATTTCA AATTGAGCAA ATTGCAGGTG
    AAGAAGATAA TGCAATTCAT TAATGGCTGG AGGCCCAAAA TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu104569
    Clone ID Related Accession (Same CDS sequence) XM_024451234.1 , XM_024451235.1
    Accession Version XM_024451235.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product GRB2-associated and regulator of MAPK protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
    GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC
    CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG
    AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTCAT TTGTATGAAT
    CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA
    AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA
    ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC
    CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG
    GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT
    GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGAGCTGGCC CGAAACCCTG
    GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT
    GTCCGTGATG TGAAAACCGA CTGGAATGAA GAATGCAAGA GCCCCAAGAA GGGTCGGTGC
    TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC
    TTCCACCGAC TCTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC
    CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC
    TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA
    GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA GGAAGGCAAG
    CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGCGAGAAGA ACAGATGTGA TCAGTTTAGA
    GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA
    GCAGTGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC
    GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT
    TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC
    TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCAG CGTCACTAAA
    ACTGACACAA ATCCTTCTGA AAGCACTCCT GTTTCCTGCT ATCCATGTAA CCGAGTGAAA
    ACTGATTCTG TGGACCTGAA ATCCCCGTTT GGAAGTCCTT CTGCTGAAGC TGTGTCCTCT
    CGGCTCTCAT GGCCTAACCA TTATTCAGGA GCATCAGAAA GCCAGACCAG GAGTGACTTC
    CTGCTGGATC CAAGCAGGAG TTATAGTTAC CCTAGACAAA AGACGCCAGG CACACCAAAG
    AGAAACTGCC CAGCACCTTT TGATTTTGAT GGCTGTGAGC TCCTGGCCAG CCCCACTAGC
    CCAGTCACTG CAGAATTCAG TAGCAGCGTC TCTGGTTGTC CCAAGTCAGC CAGCTACTCT
    CTGGAGAGCA CAGATGTGAA ATCTCTTGCA GCTGGTGTGA CAAAGCAGAG TACGTCATGC
    CCTGCCTTAC CCCCCAGGGC TCCAAAACTA GTGGAAGAGA AGGTCGCCTC CGAAACATCT
    CCTTTGCCTC TGAAAATTGA TGGTGCTGAG GAAGACCCCA AGTCTGGGTC ACCAGATCTC
    TCGGAGGACC AGTATTTTGT TAAAAAGGGC ATGCAGGACA TCTTCTCTGC CTCCTACCCT
    TTCTCATCTC CGCTCCATCT CCAGCTGGCC CCCAGATCCT GTGGCGACGG TTCCCCATGG
    CAGCCACCTG CTGACCTATC AGGACTCTCT ATAGAGGAAG TGTCCAAGTC ACTACGGTTC
    ATTGGTTTGT CCGAAGATGT CATATCATTC TTTGTTACTG AAAAGATTGA TGGGAACCTG
    CTTGTTCAGC TAACGGAAGA AATCCTCTCA GAGGATTTCA AATTGAGCAA ATTGCAGGTG
    AAGAAGATAA TGCAATTCAT TAATGGCTGG AGGCCCAAAA TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024307003.1
    CDS503..2767
    Translation

    Target ORF information:

    RefSeq Version XM_024451235.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024451235.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
    GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC
    CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG
    AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTCAT TTGTATGAAT
    CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA
    AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA
    ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC
    CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG
    GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT
    GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGAGCTGGCC CGAAACCCTG
    GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT
    GTCCGTGATG TGAAAACCGA CTGGAATGAA GAATGCAAGA GCCCCAAGAA GGGTCGGTGC
    TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC
    TTCCACCGAC TCTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC
    CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC
    TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA
    GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA GGAAGGCAAG
    CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGCGAGAAGA ACAGATGTGA TCAGTTTAGA
    GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA
    GCAGTGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC
    GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT
    TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC
    TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCAG CGTCACTAAA
    ACTGACACAA ATCCTTCTGA AAGCACTCCT GTTTCCTGCT ATCCATGTAA CCGAGTGAAA
    ACTGATTCTG TGGACCTGAA ATCCCCGTTT GGAAGTCCTT CTGCTGAAGC TGTGTCCTCT
    CGGCTCTCAT GGCCTAACCA TTATTCAGGA GCATCAGAAA GCCAGACCAG GAGTGACTTC
    CTGCTGGATC CAAGCAGGAG TTATAGTTAC CCTAGACAAA AGACGCCAGG CACACCAAAG
    AGAAACTGCC CAGCACCTTT TGATTTTGAT GGCTGTGAGC TCCTGGCCAG CCCCACTAGC
    CCAGTCACTG CAGAATTCAG TAGCAGCGTC TCTGGTTGTC CCAAGTCAGC CAGCTACTCT
    CTGGAGAGCA CAGATGTGAA ATCTCTTGCA GCTGGTGTGA CAAAGCAGAG TACGTCATGC
    CCTGCCTTAC CCCCCAGGGC TCCAAAACTA GTGGAAGAGA AGGTCGCCTC CGAAACATCT
    CCTTTGCCTC TGAAAATTGA TGGTGCTGAG GAAGACCCCA AGTCTGGGTC ACCAGATCTC
    TCGGAGGACC AGTATTTTGT TAAAAAGGGC ATGCAGGACA TCTTCTCTGC CTCCTACCCT
    TTCTCATCTC CGCTCCATCT CCAGCTGGCC CCCAGATCCT GTGGCGACGG TTCCCCATGG
    CAGCCACCTG CTGACCTATC AGGACTCTCT ATAGAGGAAG TGTCCAAGTC ACTACGGTTC
    ATTGGTTTGT CCGAAGATGT CATATCATTC TTTGTTACTG AAAAGATTGA TGGGAACCTG
    CTTGTTCAGC TAACGGAAGA AATCCTCTCA GAGGATTTCA AATTGAGCAA ATTGCAGGTG
    AAGAAGATAA TGCAATTCAT TAATGGCTGG AGGCCCAAAA TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu104570
    Clone ID Related Accession (Same CDS sequence) XM_024451236.1
    Accession Version XM_024451236.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product GRB2-associated and regulator of MAPK protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
    GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC
    CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG
    AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTCAT TTGTATGAAT
    CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA
    AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA
    ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC
    CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG
    GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT
    GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGAGCTGGCC CGAAACCCTG
    GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT
    GTCCGTGATG TGAAAACCGA CTGGAATGAA GAATGCAAGA GCCCCAAGAA GGGTCGGTGC
    TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC
    TTCCACCGAC TCTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC
    CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC
    TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA
    GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA GGAAGGCAAG
    CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGCGAGAAGA ACAGATGTGA TCAGTTTAGA
    GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA
    GCAGTGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC
    GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT
    TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC
    TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCGT CACTAAAACT
    GACACAAATC CTTCTGAAAG CACTCCTGTT TCCTGCTATC CATGTAACCG AGTGAAAACT
    GATTCTGTGG ACCTGAAATC CCCGTTTGGA AGTCCTTCTG CTGAAGCTGT GTCCTCTCGG
    CTCTCATGGC CTAACCATTA TTCAGGAGCA TCAGAAAGCC AGACCAGGAG TGACTTCCTG
    CTGGATCCAA GCAGGAGTTA TAGTTACCCT AGACAAAAGA CGCCAGGCAC ACCAAAGAGA
    AACTGCCCAG CACCTTTTGA TTTTGATGGC TGTGAGCTCC TGGCCAGCCC CACTAGCCCA
    GTCACTGCAG AATTCAGTAG CAGCGTCTCT GGTTGTCCCA AGTCAGCCAG CTACTCTCTG
    GAGAGCACAG ATGTGAAATC TCTTGCAGCT GGTGTGACAA AGCAGAGTAC GTCATGCCCT
    GCCTTACCCC CCAGGGCTCC AAAACTAGTG GAAGAGAAGG TCGCCTCCGA AACATCTCCT
    TTGCCTCTGA AAATTGATGG TGCTGAGGAA GACCCCAAGT CTGGGTCACC AGATCTCTCG
    GAGGACCAGT ATTTTGTTAA AAAGGGCATG CAGGACATCT TCTCTGCCTC CTACCCTTTC
    TCATCTCCGC TCCATCTCCA GCTGGCCCCC AGATCCTGTG GCGACGGTTC CCCATGGCAG
    CCACCTGCTG ACCTATCAGG ACTCTCTATA GAGGAAGTGT CCAAGTCACT ACGGTTCATT
    GGTTTGTCCG AAGATGTCAT ATCATTCTTT GTTACTGAAA AGATTGATGG GAACCTGCTT
    GTTCAGCTAA CGGAAGAAAT CCTCTCAGAG GATTTCAAAT TGAGCAAATT GCAGGTGAAG
    AAGATAATGC AATTCATTAA TGGCTGGAGG CCCAAAATAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024307004.1
    CDS569..2830
    Translation

    Target ORF information:

    RefSeq Version XM_024451236.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024451236.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
    GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC
    CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG
    AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTCAT TTGTATGAAT
    CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA
    AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA
    ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC
    CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG
    GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT
    GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGAGCTGGCC CGAAACCCTG
    GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT
    GTCCGTGATG TGAAAACCGA CTGGAATGAA GAATGCAAGA GCCCCAAGAA GGGTCGGTGC
    TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC
    TTCCACCGAC TCTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC
    CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC
    TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA
    GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA GGAAGGCAAG
    CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGCGAGAAGA ACAGATGTGA TCAGTTTAGA
    GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA
    GCAGTGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC
    GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT
    TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC
    TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCGT CACTAAAACT
    GACACAAATC CTTCTGAAAG CACTCCTGTT TCCTGCTATC CATGTAACCG AGTGAAAACT
    GATTCTGTGG ACCTGAAATC CCCGTTTGGA AGTCCTTCTG CTGAAGCTGT GTCCTCTCGG
    CTCTCATGGC CTAACCATTA TTCAGGAGCA TCAGAAAGCC AGACCAGGAG TGACTTCCTG
    CTGGATCCAA GCAGGAGTTA TAGTTACCCT AGACAAAAGA CGCCAGGCAC ACCAAAGAGA
    AACTGCCCAG CACCTTTTGA TTTTGATGGC TGTGAGCTCC TGGCCAGCCC CACTAGCCCA
    GTCACTGCAG AATTCAGTAG CAGCGTCTCT GGTTGTCCCA AGTCAGCCAG CTACTCTCTG
    GAGAGCACAG ATGTGAAATC TCTTGCAGCT GGTGTGACAA AGCAGAGTAC GTCATGCCCT
    GCCTTACCCC CCAGGGCTCC AAAACTAGTG GAAGAGAAGG TCGCCTCCGA AACATCTCCT
    TTGCCTCTGA AAATTGATGG TGCTGAGGAA GACCCCAAGT CTGGGTCACC AGATCTCTCG
    GAGGACCAGT ATTTTGTTAA AAAGGGCATG CAGGACATCT TCTCTGCCTC CTACCCTTTC
    TCATCTCCGC TCCATCTCCA GCTGGCCCCC AGATCCTGTG GCGACGGTTC CCCATGGCAG
    CCACCTGCTG ACCTATCAGG ACTCTCTATA GAGGAAGTGT CCAAGTCACT ACGGTTCATT
    GGTTTGTCCG AAGATGTCAT ATCATTCTTT GTTACTGAAA AGATTGATGG GAACCTGCTT
    GTTCAGCTAA CGGAAGAAAT CCTCTCAGAG GATTTCAAAT TGAGCAAATT GCAGGTGAAG
    AAGATAATGC AATTCATTAA TGGCTGGAGG CCCAAAATAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.