garem1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following garem1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the garem1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa11586 XM_002934118.4
Latest version!
Xenopus tropicalis GRB2 associated regulator of MAPK1 subtype 1 (garem1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa11587 XM_004915201.3
Latest version!
Xenopus tropicalis GRB2 associated regulator of MAPK1 subtype 1 (garem1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa60002 XM_002934118.5
Latest version!
Xenopus tropicalis GRB2 associated regulator of MAPK1 subtype 1 (garem1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa60003 XM_004915201.4
Latest version!
Xenopus tropicalis GRB2 associated regulator of MAPK1 subtype 1 (garem1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa60004 XM_031903647.1
Latest version!
Xenopus tropicalis GRB2 associated regulator of MAPK1 subtype 1 (garem1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa60005 XM_031903648.1
Latest version!
Xenopus tropicalis GRB2 associated regulator of MAPK1 subtype 1 (garem1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa11586
    Clone ID Related Accession (Same CDS sequence) XM_002934118.4
    Accession Version XM_002934118.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2640bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GRB2-associated and regulator of MAPK protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934118.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAACCCC CTGTCCCCCC TCTGAAGGAT GTCAAGTGGA GCTCGGCCTC CTTCCCTCTG 
    GACCTGCTTG TGAGCTCCTA TCGGCTACCC CAGCTTGCCC GGGCAGACTG CGCAGGAGAA
    AACATTGAAG GCTTGCGGGA GAATGACTGT CTCTTGATTC ACTCCTGTCG GCAATGGACA
    ACCATCACTG CTCACAGTTT AGAAGAGGGG CATTATGTCA TTGGTCCAAA GATTGAGATT
    CCTGTGCATT ATGGAGGGCA GTTTAAGCTG CTGGAACAGG ACCGGGATAT AAAGGAGCCA
    GTACAGTATT TTAACAGCGT GGAGGAGGTG GCAAAAGCAT TTCCTGAGCG AGTATACGTC
    ATGGAGGAAA TAACATTTAA TGTTAAGGTA GCTTCAGGAG AGTGCAATGA AGATACTGAG
    GTTTACAATA TTACGCTGTG CACTGGGGAT GAGCTAACAT TAATGGGGCA GGCAGAAATT
    CTTTATGCAA AAACATCCAA GGAGAAGTCC CGACTCAATA CCATTTTTAA GAAGATTGGA
    AAGCTGAACT CGATCAGCAA ACTGGGCAAA GGCAAAATGC CCTGCCTCAT ATGCATGAAC
    CATAGAACGA ACGAAAGCAT CAGCCTCCCA TTTCAGTGTA AGGGAAGGTT CAGCACTCGC
    AGCCCTCTGG AGATTCAGAT GCAGGAAGGA GAACACACGA TACGCAACAT TGTGGAGAAA
    ACAAGATTGC CCGTAAATGT AACTGTGCCT AGCCCACCCC CTAGAAATCC CTATGACCTT
    CATTTCATAC GGGAAGGGCA TCGGTACAAA TTTGTCAACA TCCAGACCAA GACTGTTGTA
    GTTTGCTGTG TGTTACAGGG CAATAAAATA ATCCCCATGC ATTTTCCATT GCATTTATCA
    GTCCCAAAGT TTAGCCTTCC AGAAAATCTC GTCAAGGGAG AAGTGTGGCA AGACTCTGTA
    GTGCAGCACT GGTACAACAC ATGCCAAGAA CAATTTGATA TAGAAGAATA CTCTCGGGCA
    GTGAGGGATG TGAAAGCAGA CTGGAGTGAA GAGTGCAAAA GCCCTAAAAA GACATGGTGT
    TCTGGCCACA GCCATATACC AAATTCCCTC AGTTACGCTC GGGATGAACT GACTCAGTCA
    TTCCATAGGC TTTCAGTTTG TGTCTATGGA AACAATCTTC ACGGAAACAG TGAGGTGAAT
    CTGCATGGCT GCAGAGATCT TTGCGGGGAT TGGGCCGTTT TTCCTCAAGA TTCCCCACAG
    TATCAGGACT CGTGTGATAG TGGAAGTGAT TACCTTTTCC CTGAAAGCAG TGAAGAACTA
    ACAAGTCTAC CAGCCAAGCC AGAACTTCCT TATGAGGAGT TATGGCTAGA ACAAGGATCA
    GTTAAAAGGA GTGCGCAGCC TCTCACACGG TCACAAAGTG AGAAAAACAA ATGTGATCCA
    TTCAGAGGTT CACTTCCCTC TCGGTGCGGC ACGTCATCTC TTCCCTCTCC TGGATCTCTT
    CTGACAACTA AGAGTTTGGA TGTATCCCTA CCTCCACCTC CAGTGCCTCC AAAATCTGAA
    GCTGTAAAAG AAGAATGCAG ACTTCTGAAT GCTCCGCCTG TGCCTCCACG CAGTTCCAAG
    CCATCATCCC CCACTCCATC TGTTTCACCA GCGGCTGGAA AAATTGCACG GCAGCAAACA
    AGATCCCCCA GCCCTACCCT TTCTTACTAT TCATCAGGTC TGCATAGCAT AGGCATCACG
    GAAAGTGAAA CGACCAACTC GTCAGATAAT TCTCAAGTTT CCTGCTATCC ATGTAACTGG
    ATAAAAAACG AAAGCGTTGG TCTTGAAAGC GATTTAACTT GTGGTAGCAC ATCGTCAGAA
    GCCCTGCCTT CCCGATTATC CTGGCCAAAC CGATATTGTG GTGGGGCAGA AGGCCTTAAC
    GTGAATGATT TACCTATGGA TCAGTGTCGC AGTTATTATA GTTACCCTCG ACAAAAGACA
    CCAGGAACAC CAAAGAAAAA TTGTCCAGCT CCATTTGACT TTGGTGGCTG TGGTTCTTTT
    ACTGGGTGCA ACCAGGTGGC ACAAGGTGAC CTCTCAGACA TTGTCCCAGG CTGTCCAAAG
    TCTGCAAGCT ACTCACTAGA AAGTTCCACT GATAGAATGA TATCTGACAA TAGCACGAAA
    CAAAGCTTGT CATGTCCTGC CTTACCACCT CGTGCACCAA AATCCAGTGA AGGCAATGCT
    CTCTTGGAAC CCTTGTCTCT GCCTATGAAG ATTGATGGAG CCGAAGAGGA AATTCAGACT
    TGCTCTCCAG ACTTTTCAGA AGAACATTAT CTTGCTAAAA AGGGTGTTCA GGATATTTTC
    TCTATTTCCT ACCCATTCTC CTCACCACTT CACATGCAGC TAGCTCCTAG GTCTTGCGGG
    GATGGGTCTC CTTGGCAACC ACCAACTGAT CTTTCAGGAC TCTCGGTTGA AGAGGTGTCA
    AAATCCCTTC GATTCATTGG TTTGTCAGAG GACGTGGTGT CATTTTTTGT TTCGGAAAAA
    ATTGATGGAA ATTTACTTGT CCAGCTAACA GAAGAAATAC TCTCAGAGGA CTTTAAGTTA
    AGCAAGTTAC AGGTGAAAAA ATTATTACAA TTTATTAACG GCTGGCGACC GAAGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934164.1
    CDS424..3063
    Translation

    Target ORF information:

    RefSeq Version XM_002934118.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GRB2 associated regulator of MAPK1 subtype 1 (garem1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934118.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAACCCC CTGTCCCCCC TCTGAAGGAT GTCAAGTGGA GCTCGGCCTC CTTCCCTCTG 
    GACCTGCTTG TGAGCTCCTA TCGGCTACCC CAGCTTGCCC GGGCAGACTG CGCAGGAGAA
    AACATTGAAG GCTTGCGGGA GAATGACTGT CTCTTGATTC ACTCCTGTCG GCAATGGACA
    ACCATCACTG CTCACAGTTT AGAAGAGGGG CATTATGTCA TTGGTCCAAA GATTGAGATT
    CCTGTGCATT ATGGAGGGCA GTTTAAGCTG CTGGAACAGG ACCGGGATAT AAAGGAGCCA
    GTACAGTATT TTAACAGCGT GGAGGAGGTG GCAAAAGCAT TTCCTGAGCG AGTATACGTC
    ATGGAGGAAA TAACATTTAA TGTTAAGGTA GCTTCAGGAG AGTGCAATGA AGATACTGAG
    GTTTACAATA TTACGCTGTG CACTGGGGAT GAGCTAACAT TAATGGGGCA GGCAGAAATT
    CTTTATGCAA AAACATCCAA GGAGAAGTCC CGACTCAATA CCATTTTTAA GAAGATTGGA
    AAGCTGAACT CGATCAGCAA ACTGGGCAAA GGCAAAATGC CCTGCCTCAT ATGCATGAAC
    CATAGAACGA ACGAAAGCAT CAGCCTCCCA TTTCAGTGTA AGGGAAGGTT CAGCACTCGC
    AGCCCTCTGG AGATTCAGAT GCAGGAAGGA GAACACACGA TACGCAACAT TGTGGAGAAA
    ACAAGATTGC CCGTAAATGT AACTGTGCCT AGCCCACCCC CTAGAAATCC CTATGACCTT
    CATTTCATAC GGGAAGGGCA TCGGTACAAA TTTGTCAACA TCCAGACCAA GACTGTTGTA
    GTTTGCTGTG TGTTACAGGG CAATAAAATA ATCCCCATGC ATTTTCCATT GCATTTATCA
    GTCCCAAAGT TTAGCCTTCC AGAAAATCTC GTCAAGGGAG AAGTGTGGCA AGACTCTGTA
    GTGCAGCACT GGTACAACAC ATGCCAAGAA CAATTTGATA TAGAAGAATA CTCTCGGGCA
    GTGAGGGATG TGAAAGCAGA CTGGAGTGAA GAGTGCAAAA GCCCTAAAAA GACATGGTGT
    TCTGGCCACA GCCATATACC AAATTCCCTC AGTTACGCTC GGGATGAACT GACTCAGTCA
    TTCCATAGGC TTTCAGTTTG TGTCTATGGA AACAATCTTC ACGGAAACAG TGAGGTGAAT
    CTGCATGGCT GCAGAGATCT TTGCGGGGAT TGGGCCGTTT TTCCTCAAGA TTCCCCACAG
    TATCAGGACT CGTGTGATAG TGGAAGTGAT TACCTTTTCC CTGAAAGCAG TGAAGAACTA
    ACAAGTCTAC CAGCCAAGCC AGAACTTCCT TATGAGGAGT TATGGCTAGA ACAAGGATCA
    GTTAAAAGGA GTGCGCAGCC TCTCACACGG TCACAAAGTG AGAAAAACAA ATGTGATCCA
    TTCAGAGGTT CACTTCCCTC TCGGTGCGGC ACGTCATCTC TTCCCTCTCC TGGATCTCTT
    CTGACAACTA AGAGTTTGGA TGTATCCCTA CCTCCACCTC CAGTGCCTCC AAAATCTGAA
    GCTGTAAAAG AAGAATGCAG ACTTCTGAAT GCTCCGCCTG TGCCTCCACG CAGTTCCAAG
    CCATCATCCC CCACTCCATC TGTTTCACCA GCGGCTGGAA AAATTGCACG GCAGCAAACA
    AGATCCCCCA GCCCTACCCT TTCTTACTAT TCATCAGGTC TGCATAGCAT AGGCATCACG
    GAAAGTGAAA CGACCAACTC GTCAGATAAT TCTCAAGTTT CCTGCTATCC ATGTAACTGG
    ATAAAAAACG AAAGCGTTGG TCTTGAAAGC GATTTAACTT GTGGTAGCAC ATCGTCAGAA
    GCCCTGCCTT CCCGATTATC CTGGCCAAAC CGATATTGTG GTGGGGCAGA AGGCCTTAAC
    GTGAATGATT TACCTATGGA TCAGTGTCGC AGTTATTATA GTTACCCTCG ACAAAAGACA
    CCAGGAACAC CAAAGAAAAA TTGTCCAGCT CCATTTGACT TTGGTGGCTG TGGTTCTTTT
    ACTGGGTGCA ACCAGGTGGC ACAAGGTGAC CTCTCAGACA TTGTCCCAGG CTGTCCAAAG
    TCTGCAAGCT ACTCACTAGA AAGTTCCACT GATAGAATGA TATCTGACAA TAGCACGAAA
    CAAAGCTTGT CATGTCCTGC CTTACCACCT CGTGCACCAA AATCCAGTGA AGGCAATGCT
    CTCTTGGAAC CCTTGTCTCT GCCTATGAAG ATTGATGGAG CCGAAGAGGA AATTCAGACT
    TGCTCTCCAG ACTTTTCAGA AGAACATTAT CTTGCTAAAA AGGGTGTTCA GGATATTTTC
    TCTATTTCCT ACCCATTCTC CTCACCACTT CACATGCAGC TAGCTCCTAG GTCTTGCGGG
    GATGGGTCTC CTTGGCAACC ACCAACTGAT CTTTCAGGAC TCTCGGTTGA AGAGGTGTCA
    AAATCCCTTC GATTCATTGG TTTGTCAGAG GACGTGGTGT CATTTTTTGT TTCGGAAAAA
    ATTGATGGAA ATTTACTTGT CCAGCTAACA GAAGAAATAC TCTCAGAGGA CTTTAAGTTA
    AGCAAGTTAC AGGTGAAAAA ATTATTACAA TTTATTAACG GCTGGCGACC GAAGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa11587
    Clone ID Related Accession (Same CDS sequence) XM_004915201.3
    Accession Version XM_004915201.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GRB2-associated and regulator of MAPK protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915201.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAACCCC CTGTCCCCCC TCTGAAGGAT GTCAAGTGGA GCTCGGCCTC CTTCCCTCTG 
    GACCTGCTTG TGAGCTCCTA TCGGCTACCC CAGCTTGCCC GGGCAGACTG CGGAGAAAAC
    ATTGAAGGCT TGCGGGAGAA TGACTGTCTC TTGATTCACT CCTGTCGGCA ATGGACAACC
    ATCACTGCTC ACAGTTTAGA AGAGGGGCAT TATGTCATTG GTCCAAAGAT TGAGATTCCT
    GTGCATTATG GAGGGCAGTT TAAGCTGCTG GAACAGGACC GGGATATAAA GGAGCCAGTA
    CAGTATTTTA ACAGCGTGGA GGAGGTGGCA AAAGCATTTC CTGAGCGAGT ATACGTCATG
    GAGGAAATAA CATTTAATGT TAAGGTAGCT TCAGGAGAGT GCAATGAAGA TACTGAGGTT
    TACAATATTA CGCTGTGCAC TGGGGATGAG CTAACATTAA TGGGGCAGGC AGAAATTCTT
    TATGCAAAAA CATCCAAGGA GAAGTCCCGA CTCAATACCA TTTTTAAGAA GATTGGAAAG
    CTGAACTCGA TCAGCAAACT GGGCAAAGGC AAAATGCCCT GCCTCATATG CATGAACCAT
    AGAACGAACG AAAGCATCAG CCTCCCATTT CAGTGTAAGG GAAGGTTCAG CACTCGCAGC
    CCTCTGGAGA TTCAGATGCA GGAAGGAGAA CACACGATAC GCAACATTGT GGAGAAAACA
    AGATTGCCCG TAAATGTAAC TGTGCCTAGC CCACCCCCTA GAAATCCCTA TGACCTTCAT
    TTCATACGGG AAGGGCATCG GTACAAATTT GTCAACATCC AGACCAAGAC TGTTGTAGTT
    TGCTGTGTGT TACAGGGCAA TAAAATAATC CCCATGCATT TTCCATTGCA TTTATCAGTC
    CCAAAGTTTA GCCTTCCAGA AAATCTCGTC AAGGGAGAAG TGTGGCAAGA CTCTGTAGTG
    CAGCACTGGT ACAACACATG CCAAGAACAA TTTGATATAG AAGAATACTC TCGGGCAGTG
    AGGGATGTGA AAGCAGACTG GAGTGAAGAG TGCAAAAGCC CTAAAAAGAC ATGGTGTTCT
    GGCCACAGCC ATATACCAAA TTCCCTCAGT TACGCTCGGG ATGAACTGAC TCAGTCATTC
    CATAGGCTTT CAGTTTGTGT CTATGGAAAC AATCTTCACG GAAACAGTGA GGTGAATCTG
    CATGGCTGCA GAGATCTTTG CGGGGATTGG GCCGTTTTTC CTCAAGATTC CCCACAGTAT
    CAGGACTCGT GTGATAGTGG AAGTGATTAC CTTTTCCCTG AAAGCAGTGA AGAACTAACA
    AGTCTACCAG CCAAGCCAGA ACTTCCTTAT GAGGAGTTAT GGCTAGAACA AGGATCAGTT
    AAAAGGAGTG CGCAGCCTCT CACACGGTCA CAAAGTGAGA AAAACAAATG TGATCCATTC
    AGAGGTTCAC TTCCCTCTCG GTGCGGCACG TCATCTCTTC CCTCTCCTGG ATCTCTTCTG
    ACAACTAAGA GTTTGGATGT ATCCCTACCT CCACCTCCAG TGCCTCCAAA ATCTGAAGCT
    GTAAAAGAAG AATGCAGACT TCTGAATGCT CCGCCTGTGC CTCCACGCAG TTCCAAGCCA
    TCATCCCCCA CTCCATCTGT TTCACCAGCG GCTGGAAAAA TTGCACGGCA GCAAACAAGA
    TCCCCCAGCC CTACCCTTTC TTACTATTCA TCAGGTCTGC ATAGCATAGG CATCACGGAA
    AGTGAAACGA CCAACTCGTC AGATAATTCT CAAGTTTCCT GCTATCCATG TAACTGGATA
    AAAAACGAAA GCGTTGGTCT TGAAAGCGAT TTAACTTGTG GTAGCACATC GTCAGAAGCC
    CTGCCTTCCC GATTATCCTG GCCAAACCGA TATTGTGGTG GGGCAGAAGG CCTTAACGTG
    AATGATTTAC CTATGGATCA GTGTCGCAGT TATTATAGTT ACCCTCGACA AAAGACACCA
    GGAACACCAA AGAAAAATTG TCCAGCTCCA TTTGACTTTG GTGGCTGTGG TTCTTTTACT
    GGGTGCAACC AGGTGGCACA AGGTGACCTC TCAGACATTG TCCCAGGCTG TCCAAAGTCT
    GCAAGCTACT CACTAGAAAG TTCCACTGAT AGAATGATAT CTGACAATAG CACGAAACAA
    AGCTTGTCAT GTCCTGCCTT ACCACCTCGT GCACCAAAAT CCAGTGAAGG CAATGCTCTC
    TTGGAACCCT TGTCTCTGCC TATGAAGATT GATGGAGCCG AAGAGGAAAT TCAGACTTGC
    TCTCCAGACT TTTCAGAAGA ACATTATCTT GCTAAAAAGG GTGTTCAGGA TATTTTCTCT
    ATTTCCTACC CATTCTCCTC ACCACTTCAC ATGCAGCTAG CTCCTAGGTC TTGCGGGGAT
    GGGTCTCCTT GGCAACCACC AACTGATCTT TCAGGACTCT CGGTTGAAGA GGTGTCAAAA
    TCCCTTCGAT TCATTGGTTT GTCAGAGGAC GTGGTGTCAT TTTTTGTTTC GGAAAAAATT
    GATGGAAATT TACTTGTCCA GCTAACAGAA GAAATACTCT CAGAGGACTT TAAGTTAAGC
    AAGTTACAGG TGAAAAAATT ATTACAATTT ATTAACGGCT GGCGACCGAA GATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004915258.1
    CDS425..3061
    Translation

    Target ORF information:

    RefSeq Version XM_004915201.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GRB2 associated regulator of MAPK1 subtype 1 (garem1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915201.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAACCCC CTGTCCCCCC TCTGAAGGAT GTCAAGTGGA GCTCGGCCTC CTTCCCTCTG 
    GACCTGCTTG TGAGCTCCTA TCGGCTACCC CAGCTTGCCC GGGCAGACTG CGGAGAAAAC
    ATTGAAGGCT TGCGGGAGAA TGACTGTCTC TTGATTCACT CCTGTCGGCA ATGGACAACC
    ATCACTGCTC ACAGTTTAGA AGAGGGGCAT TATGTCATTG GTCCAAAGAT TGAGATTCCT
    GTGCATTATG GAGGGCAGTT TAAGCTGCTG GAACAGGACC GGGATATAAA GGAGCCAGTA
    CAGTATTTTA ACAGCGTGGA GGAGGTGGCA AAAGCATTTC CTGAGCGAGT ATACGTCATG
    GAGGAAATAA CATTTAATGT TAAGGTAGCT TCAGGAGAGT GCAATGAAGA TACTGAGGTT
    TACAATATTA CGCTGTGCAC TGGGGATGAG CTAACATTAA TGGGGCAGGC AGAAATTCTT
    TATGCAAAAA CATCCAAGGA GAAGTCCCGA CTCAATACCA TTTTTAAGAA GATTGGAAAG
    CTGAACTCGA TCAGCAAACT GGGCAAAGGC AAAATGCCCT GCCTCATATG CATGAACCAT
    AGAACGAACG AAAGCATCAG CCTCCCATTT CAGTGTAAGG GAAGGTTCAG CACTCGCAGC
    CCTCTGGAGA TTCAGATGCA GGAAGGAGAA CACACGATAC GCAACATTGT GGAGAAAACA
    AGATTGCCCG TAAATGTAAC TGTGCCTAGC CCACCCCCTA GAAATCCCTA TGACCTTCAT
    TTCATACGGG AAGGGCATCG GTACAAATTT GTCAACATCC AGACCAAGAC TGTTGTAGTT
    TGCTGTGTGT TACAGGGCAA TAAAATAATC CCCATGCATT TTCCATTGCA TTTATCAGTC
    CCAAAGTTTA GCCTTCCAGA AAATCTCGTC AAGGGAGAAG TGTGGCAAGA CTCTGTAGTG
    CAGCACTGGT ACAACACATG CCAAGAACAA TTTGATATAG AAGAATACTC TCGGGCAGTG
    AGGGATGTGA AAGCAGACTG GAGTGAAGAG TGCAAAAGCC CTAAAAAGAC ATGGTGTTCT
    GGCCACAGCC ATATACCAAA TTCCCTCAGT TACGCTCGGG ATGAACTGAC TCAGTCATTC
    CATAGGCTTT CAGTTTGTGT CTATGGAAAC AATCTTCACG GAAACAGTGA GGTGAATCTG
    CATGGCTGCA GAGATCTTTG CGGGGATTGG GCCGTTTTTC CTCAAGATTC CCCACAGTAT
    CAGGACTCGT GTGATAGTGG AAGTGATTAC CTTTTCCCTG AAAGCAGTGA AGAACTAACA
    AGTCTACCAG CCAAGCCAGA ACTTCCTTAT GAGGAGTTAT GGCTAGAACA AGGATCAGTT
    AAAAGGAGTG CGCAGCCTCT CACACGGTCA CAAAGTGAGA AAAACAAATG TGATCCATTC
    AGAGGTTCAC TTCCCTCTCG GTGCGGCACG TCATCTCTTC CCTCTCCTGG ATCTCTTCTG
    ACAACTAAGA GTTTGGATGT ATCCCTACCT CCACCTCCAG TGCCTCCAAA ATCTGAAGCT
    GTAAAAGAAG AATGCAGACT TCTGAATGCT CCGCCTGTGC CTCCACGCAG TTCCAAGCCA
    TCATCCCCCA CTCCATCTGT TTCACCAGCG GCTGGAAAAA TTGCACGGCA GCAAACAAGA
    TCCCCCAGCC CTACCCTTTC TTACTATTCA TCAGGTCTGC ATAGCATAGG CATCACGGAA
    AGTGAAACGA CCAACTCGTC AGATAATTCT CAAGTTTCCT GCTATCCATG TAACTGGATA
    AAAAACGAAA GCGTTGGTCT TGAAAGCGAT TTAACTTGTG GTAGCACATC GTCAGAAGCC
    CTGCCTTCCC GATTATCCTG GCCAAACCGA TATTGTGGTG GGGCAGAAGG CCTTAACGTG
    AATGATTTAC CTATGGATCA GTGTCGCAGT TATTATAGTT ACCCTCGACA AAAGACACCA
    GGAACACCAA AGAAAAATTG TCCAGCTCCA TTTGACTTTG GTGGCTGTGG TTCTTTTACT
    GGGTGCAACC AGGTGGCACA AGGTGACCTC TCAGACATTG TCCCAGGCTG TCCAAAGTCT
    GCAAGCTACT CACTAGAAAG TTCCACTGAT AGAATGATAT CTGACAATAG CACGAAACAA
    AGCTTGTCAT GTCCTGCCTT ACCACCTCGT GCACCAAAAT CCAGTGAAGG CAATGCTCTC
    TTGGAACCCT TGTCTCTGCC TATGAAGATT GATGGAGCCG AAGAGGAAAT TCAGACTTGC
    TCTCCAGACT TTTCAGAAGA ACATTATCTT GCTAAAAAGG GTGTTCAGGA TATTTTCTCT
    ATTTCCTACC CATTCTCCTC ACCACTTCAC ATGCAGCTAG CTCCTAGGTC TTGCGGGGAT
    GGGTCTCCTT GGCAACCACC AACTGATCTT TCAGGACTCT CGGTTGAAGA GGTGTCAAAA
    TCCCTTCGAT TCATTGGTTT GTCAGAGGAC GTGGTGTCAT TTTTTGTTTC GGAAAAAATT
    GATGGAAATT TACTTGTCCA GCTAACAGAA GAAATACTCT CAGAGGACTT TAAGTTAAGC
    AAGTTACAGG TGAAAAAATT ATTACAATTT ATTAACGGCT GGCGACCGAA GATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60002
    Clone ID Related Accession (Same CDS sequence) XM_002934118.5
    Accession Version XM_002934118.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2640bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GRB2-associated and regulator of MAPK protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934118.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAACCCC CTGTCCCCCC TCTGAAGGAT GTCAAGTGGA GCTCGGCCTC CTTCCCTCTG 
    GACCTGCTTG TGAGCTCCTA TCGGCTACCC CAGCTTGCCC GGGCAGACTG CGCAGGAGAA
    AACATTGAAG GCTTGCGGGA GAATGACTGT CTCTTGATTC ACTCCTGTCG GCAATGGACA
    ACCATCACTG CTCACAGTTT AGAAGAGGGG CATTATGTCA TTGGTCCAAA GATTGAGATT
    CCTGTGCATT ATGGAGGGCA GTTTAAGCTG CTGGAACAGG ACCGGGATAT AAAGGAGCCA
    GTACAGTATT TTAACAGCGT GGAGGAGGTG GCAAAAGCAT TTCCTGAGCG AGTATACGTC
    ATGGAGGAAA TAACATTTAA TGTTAAGGTA GCTTCAGGAG AGTGCAATGA AGATACTGAG
    GTTTACAATA TTACGCTGTG CACTGGGGAT GAGCTAACAT TAATGGGGCA GGCAGAAATT
    CTTTATGCAA AAACATCCAA GGAGAAGTCC CGACTCAATA CCATTTTTAA GAAGATTGGA
    AAGCTGAACT CGATCAGCAA ACTGGGCAAA GGCAAAATGC CCTGCCTCAT ATGCATGAAC
    CATAGAACGA ACGAAAGCAT CAGCCTCCCG TTTCAGTGTA AGGGAAGGTT CAGCACTCGC
    AGCCCTCTGG AGATTCAGAT GCAGGAAGGA GAACACACGA TACGCAACAT TGTGGAGAAA
    ACAAGATTGC CCGTAAATGT AACTGTGCCT AGCCCACCCC CTAGAAATCC CTATGACCTT
    CATTTCATAC GGGAAGGGCA TCGGTACAAA TTTGTCAACA TCCAGACCAA GACTGTTGTA
    GTTTGCTGTG TGTTACAGGG CAATAAAATA ATCCCCATGC ATTTTCCATT GCATTTATCA
    GTCCCAAAGT TTAGCCTTCC AGAAAATCTC GTCAAGGGAG AAGTGTGGCA AGACTCTGTA
    GTGCAGCACT GGTACAACAC ATGCCAAGAA CAATTTGATA TAGAAGAATA CTCTCGGGCA
    GTGAGGGATG TGAAAGCAGA CTGGAGTGAA GAGTGCAAAA GCCCTAAAAA GACATGGTGT
    TCTGGCCACA GCCATATACC AAATTCCCTC AGTTACGCTC GGGATGAACT GACTCAGTCA
    TTCCATAGGC TTTCAGTTTG TGTCTATGGA AACAATCTTC ACGGAAACAG TGAGGTGAAT
    CTGCATGGCT GCAGAGATCT TTGCGGGGAT TGGGCCGTTT TTCCTCAAGA TTCCCCACAG
    TATCAGGACT CGTGTGATAG TGGAAGTGAT TACCTTTTCC CTGAAAGCAG TGAAGAACTA
    ACAAGTCTAC CAGCCAAGCC AGAACTTCCT TATGAGGAGT TATGGCTAGA ACAAGGATCA
    GTTAAAAGGA GTGCGCAGCC TCTCACACGG TCACAAAGTG AGAAAAACAA ATGTGATCCA
    TTCAGAGGTT CACTTCCCTC TCGGTGCGGC ACGTCATCTC TTCCCTCTCC TGGATCTCTT
    CTGACAACTA AGAGTTTGGA TGTATCCCTA CCTCCACCTC CAGTGCCTCC AAAATCTGAA
    GCTGTAAAAG AAGAATGCAG ACTTCTGAAT GCTCCGCCTG TGCCTCCACG CAGTTCCAAG
    CCATCATCCC CCACTCCATC TGTTTCACCA GCGGCTGGAA AAATTGCACG GCAGCAAACA
    AGATCCCCCA GCCCTACCCT TTCTTACTAT TCATCAGGTC TGCATAGCAT AGGCATCACG
    GAAAGTGAAA CGACCAACTC GTCAGATAAT TCTCAAGTTT CCTGCTATCC ATGTAACTGG
    ATAAAAAACG AAAGCGTTGG TCTTGAAAGC GATTTAACTT GTGGTAGCAC ATCGTCAGAA
    GCCCTGCCTT CCCGATTATC CTGGCCAAAC CGATATTGTG GTGGGGCAGA AGGCCTTAAC
    GTGAATGATT TACCTATGGA TCAGTGTCGC AGTTATTATA GTTACCCTCG ACAAAAGACA
    CCAGGAACAC CAAAGAAAAA TTGTCCAGCT CCATTTGACT TTGGTGGCTG TGGTTCTTTT
    ACTGGGTGCA ACCAGGTGGC ACAAGGTGAC CTCTCAGACA TTGTCCCAGG CTGTCCAAAG
    TCTGCAAGCT ACTCACTAGA AAGTTCCACT GATAGAATGA TATCTGACAA TAGCACGAAA
    CAAAGCTTGT CATGTCCTGC CTTACCACCT CGTGCACCAA AATCCAGTGA AGGCAATGCT
    CTCTTGGAAC CCTTGTCTCT GCCTATGAAG ATTGATGGAG CCGAAGAGGA AATTCAGACT
    TGCTCTCCAG ACTTTTCAGA AGAACATTAT CTTGCTAAAA AGGGTGTTCA GGATATTTTC
    TCTATTTCCT ACCCATTCTC CTCACCACTT CACATGCAGC TAGCTCCTAG GTCTTGCGGG
    GATGGGTCTC CTTGGCAACC ACCAACTGAT CTTTCAGGAC TCTCGGTTGA AGAGGTGTCA
    AAATCCCTTC GATTCATTGG TTTGTCAGAG GACGTGGTGT CATTTTTTGT TTCGGAAAAA
    ATTGATGGAA ATTTACTTGT CCAGCTAACA GAAGAAATAC TCTCAGAGGA CTTTAAGTTA
    AGCAAGTTAC AGGTGAAAAA ATTATTACAA TTTATTAACG GCTGGCGACC GAAGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934164.1
    CDS361..3000
    Translation

    Target ORF information:

    RefSeq Version XM_002934118.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GRB2 associated regulator of MAPK1 subtype 1 (garem1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934118.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAACCCC CTGTCCCCCC TCTGAAGGAT GTCAAGTGGA GCTCGGCCTC CTTCCCTCTG 
    GACCTGCTTG TGAGCTCCTA TCGGCTACCC CAGCTTGCCC GGGCAGACTG CGCAGGAGAA
    AACATTGAAG GCTTGCGGGA GAATGACTGT CTCTTGATTC ACTCCTGTCG GCAATGGACA
    ACCATCACTG CTCACAGTTT AGAAGAGGGG CATTATGTCA TTGGTCCAAA GATTGAGATT
    CCTGTGCATT ATGGAGGGCA GTTTAAGCTG CTGGAACAGG ACCGGGATAT AAAGGAGCCA
    GTACAGTATT TTAACAGCGT GGAGGAGGTG GCAAAAGCAT TTCCTGAGCG AGTATACGTC
    ATGGAGGAAA TAACATTTAA TGTTAAGGTA GCTTCAGGAG AGTGCAATGA AGATACTGAG
    GTTTACAATA TTACGCTGTG CACTGGGGAT GAGCTAACAT TAATGGGGCA GGCAGAAATT
    CTTTATGCAA AAACATCCAA GGAGAAGTCC CGACTCAATA CCATTTTTAA GAAGATTGGA
    AAGCTGAACT CGATCAGCAA ACTGGGCAAA GGCAAAATGC CCTGCCTCAT ATGCATGAAC
    CATAGAACGA ACGAAAGCAT CAGCCTCCCG TTTCAGTGTA AGGGAAGGTT CAGCACTCGC
    AGCCCTCTGG AGATTCAGAT GCAGGAAGGA GAACACACGA TACGCAACAT TGTGGAGAAA
    ACAAGATTGC CCGTAAATGT AACTGTGCCT AGCCCACCCC CTAGAAATCC CTATGACCTT
    CATTTCATAC GGGAAGGGCA TCGGTACAAA TTTGTCAACA TCCAGACCAA GACTGTTGTA
    GTTTGCTGTG TGTTACAGGG CAATAAAATA ATCCCCATGC ATTTTCCATT GCATTTATCA
    GTCCCAAAGT TTAGCCTTCC AGAAAATCTC GTCAAGGGAG AAGTGTGGCA AGACTCTGTA
    GTGCAGCACT GGTACAACAC ATGCCAAGAA CAATTTGATA TAGAAGAATA CTCTCGGGCA
    GTGAGGGATG TGAAAGCAGA CTGGAGTGAA GAGTGCAAAA GCCCTAAAAA GACATGGTGT
    TCTGGCCACA GCCATATACC AAATTCCCTC AGTTACGCTC GGGATGAACT GACTCAGTCA
    TTCCATAGGC TTTCAGTTTG TGTCTATGGA AACAATCTTC ACGGAAACAG TGAGGTGAAT
    CTGCATGGCT GCAGAGATCT TTGCGGGGAT TGGGCCGTTT TTCCTCAAGA TTCCCCACAG
    TATCAGGACT CGTGTGATAG TGGAAGTGAT TACCTTTTCC CTGAAAGCAG TGAAGAACTA
    ACAAGTCTAC CAGCCAAGCC AGAACTTCCT TATGAGGAGT TATGGCTAGA ACAAGGATCA
    GTTAAAAGGA GTGCGCAGCC TCTCACACGG TCACAAAGTG AGAAAAACAA ATGTGATCCA
    TTCAGAGGTT CACTTCCCTC TCGGTGCGGC ACGTCATCTC TTCCCTCTCC TGGATCTCTT
    CTGACAACTA AGAGTTTGGA TGTATCCCTA CCTCCACCTC CAGTGCCTCC AAAATCTGAA
    GCTGTAAAAG AAGAATGCAG ACTTCTGAAT GCTCCGCCTG TGCCTCCACG CAGTTCCAAG
    CCATCATCCC CCACTCCATC TGTTTCACCA GCGGCTGGAA AAATTGCACG GCAGCAAACA
    AGATCCCCCA GCCCTACCCT TTCTTACTAT TCATCAGGTC TGCATAGCAT AGGCATCACG
    GAAAGTGAAA CGACCAACTC GTCAGATAAT TCTCAAGTTT CCTGCTATCC ATGTAACTGG
    ATAAAAAACG AAAGCGTTGG TCTTGAAAGC GATTTAACTT GTGGTAGCAC ATCGTCAGAA
    GCCCTGCCTT CCCGATTATC CTGGCCAAAC CGATATTGTG GTGGGGCAGA AGGCCTTAAC
    GTGAATGATT TACCTATGGA TCAGTGTCGC AGTTATTATA GTTACCCTCG ACAAAAGACA
    CCAGGAACAC CAAAGAAAAA TTGTCCAGCT CCATTTGACT TTGGTGGCTG TGGTTCTTTT
    ACTGGGTGCA ACCAGGTGGC ACAAGGTGAC CTCTCAGACA TTGTCCCAGG CTGTCCAAAG
    TCTGCAAGCT ACTCACTAGA AAGTTCCACT GATAGAATGA TATCTGACAA TAGCACGAAA
    CAAAGCTTGT CATGTCCTGC CTTACCACCT CGTGCACCAA AATCCAGTGA AGGCAATGCT
    CTCTTGGAAC CCTTGTCTCT GCCTATGAAG ATTGATGGAG CCGAAGAGGA AATTCAGACT
    TGCTCTCCAG ACTTTTCAGA AGAACATTAT CTTGCTAAAA AGGGTGTTCA GGATATTTTC
    TCTATTTCCT ACCCATTCTC CTCACCACTT CACATGCAGC TAGCTCCTAG GTCTTGCGGG
    GATGGGTCTC CTTGGCAACC ACCAACTGAT CTTTCAGGAC TCTCGGTTGA AGAGGTGTCA
    AAATCCCTTC GATTCATTGG TTTGTCAGAG GACGTGGTGT CATTTTTTGT TTCGGAAAAA
    ATTGATGGAA ATTTACTTGT CCAGCTAACA GAAGAAATAC TCTCAGAGGA CTTTAAGTTA
    AGCAAGTTAC AGGTGAAAAA ATTATTACAA TTTATTAACG GCTGGCGACC GAAGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60003
    Clone ID Related Accession (Same CDS sequence) XM_004915201.4
    Accession Version XM_004915201.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GRB2-associated and regulator of MAPK protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915201.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAACCCC CTGTCCCCCC TCTGAAGGAT GTCAAGTGGA GCTCGGCCTC CTTCCCTCTG 
    GACCTGCTTG TGAGCTCCTA TCGGCTACCC CAGCTTGCCC GGGCAGACTG CGGAGAAAAC
    ATTGAAGGCT TGCGGGAGAA TGACTGTCTC TTGATTCACT CCTGTCGGCA ATGGACAACC
    ATCACTGCTC ACAGTTTAGA AGAGGGGCAT TATGTCATTG GTCCAAAGAT TGAGATTCCT
    GTGCATTATG GAGGGCAGTT TAAGCTGCTG GAACAGGACC GGGATATAAA GGAGCCAGTA
    CAGTATTTTA ACAGCGTGGA GGAGGTGGCA AAAGCATTTC CTGAGCGAGT ATACGTCATG
    GAGGAAATAA CATTTAATGT TAAGGTAGCT TCAGGAGAGT GCAATGAAGA TACTGAGGTT
    TACAATATTA CGCTGTGCAC TGGGGATGAG CTAACATTAA TGGGGCAGGC AGAAATTCTT
    TATGCAAAAA CATCCAAGGA GAAGTCCCGA CTCAATACCA TTTTTAAGAA GATTGGAAAG
    CTGAACTCGA TCAGCAAACT GGGCAAAGGC AAAATGCCCT GCCTCATATG CATGAACCAT
    AGAACGAACG AAAGCATCAG CCTCCCGTTT CAGTGTAAGG GAAGGTTCAG CACTCGCAGC
    CCTCTGGAGA TTCAGATGCA GGAAGGAGAA CACACGATAC GCAACATTGT GGAGAAAACA
    AGATTGCCCG TAAATGTAAC TGTGCCTAGC CCACCCCCTA GAAATCCCTA TGACCTTCAT
    TTCATACGGG AAGGGCATCG GTACAAATTT GTCAACATCC AGACCAAGAC TGTTGTAGTT
    TGCTGTGTGT TACAGGGCAA TAAAATAATC CCCATGCATT TTCCATTGCA TTTATCAGTC
    CCAAAGTTTA GCCTTCCAGA AAATCTCGTC AAGGGAGAAG TGTGGCAAGA CTCTGTAGTG
    CAGCACTGGT ACAACACATG CCAAGAACAA TTTGATATAG AAGAATACTC TCGGGCAGTG
    AGGGATGTGA AAGCAGACTG GAGTGAAGAG TGCAAAAGCC CTAAAAAGAC ATGGTGTTCT
    GGCCACAGCC ATATACCAAA TTCCCTCAGT TACGCTCGGG ATGAACTGAC TCAGTCATTC
    CATAGGCTTT CAGTTTGTGT CTATGGAAAC AATCTTCACG GAAACAGTGA GGTGAATCTG
    CATGGCTGCA GAGATCTTTG CGGGGATTGG GCCGTTTTTC CTCAAGATTC CCCACAGTAT
    CAGGACTCGT GTGATAGTGG AAGTGATTAC CTTTTCCCTG AAAGCAGTGA AGAACTAACA
    AGTCTACCAG CCAAGCCAGA ACTTCCTTAT GAGGAGTTAT GGCTAGAACA AGGATCAGTT
    AAAAGGAGTG CGCAGCCTCT CACACGGTCA CAAAGTGAGA AAAACAAATG TGATCCATTC
    AGAGGTTCAC TTCCCTCTCG GTGCGGCACG TCATCTCTTC CCTCTCCTGG ATCTCTTCTG
    ACAACTAAGA GTTTGGATGT ATCCCTACCT CCACCTCCAG TGCCTCCAAA ATCTGAAGCT
    GTAAAAGAAG AATGCAGACT TCTGAATGCT CCGCCTGTGC CTCCACGCAG TTCCAAGCCA
    TCATCCCCCA CTCCATCTGT TTCACCAGCG GCTGGAAAAA TTGCACGGCA GCAAACAAGA
    TCCCCCAGCC CTACCCTTTC TTACTATTCA TCAGGTCTGC ATAGCATAGG CATCACGGAA
    AGTGAAACGA CCAACTCGTC AGATAATTCT CAAGTTTCCT GCTATCCATG TAACTGGATA
    AAAAACGAAA GCGTTGGTCT TGAAAGCGAT TTAACTTGTG GTAGCACATC GTCAGAAGCC
    CTGCCTTCCC GATTATCCTG GCCAAACCGA TATTGTGGTG GGGCAGAAGG CCTTAACGTG
    AATGATTTAC CTATGGATCA GTGTCGCAGT TATTATAGTT ACCCTCGACA AAAGACACCA
    GGAACACCAA AGAAAAATTG TCCAGCTCCA TTTGACTTTG GTGGCTGTGG TTCTTTTACT
    GGGTGCAACC AGGTGGCACA AGGTGACCTC TCAGACATTG TCCCAGGCTG TCCAAAGTCT
    GCAAGCTACT CACTAGAAAG TTCCACTGAT AGAATGATAT CTGACAATAG CACGAAACAA
    AGCTTGTCAT GTCCTGCCTT ACCACCTCGT GCACCAAAAT CCAGTGAAGG CAATGCTCTC
    TTGGAACCCT TGTCTCTGCC TATGAAGATT GATGGAGCCG AAGAGGAAAT TCAGACTTGC
    TCTCCAGACT TTTCAGAAGA ACATTATCTT GCTAAAAAGG GTGTTCAGGA TATTTTCTCT
    ATTTCCTACC CATTCTCCTC ACCACTTCAC ATGCAGCTAG CTCCTAGGTC TTGCGGGGAT
    GGGTCTCCTT GGCAACCACC AACTGATCTT TCAGGACTCT CGGTTGAAGA GGTGTCAAAA
    TCCCTTCGAT TCATTGGTTT GTCAGAGGAC GTGGTGTCAT TTTTTGTTTC GGAAAAAATT
    GATGGAAATT TACTTGTCCA GCTAACAGAA GAAATACTCT CAGAGGACTT TAAGTTAAGC
    AAGTTACAGG TGAAAAAATT ATTACAATTT ATTAACGGCT GGCGACCGAA GATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004915258.1
    CDS361..2997
    Translation

    Target ORF information:

    RefSeq Version XM_004915201.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GRB2 associated regulator of MAPK1 subtype 1 (garem1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915201.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAACCCC CTGTCCCCCC TCTGAAGGAT GTCAAGTGGA GCTCGGCCTC CTTCCCTCTG 
    GACCTGCTTG TGAGCTCCTA TCGGCTACCC CAGCTTGCCC GGGCAGACTG CGGAGAAAAC
    ATTGAAGGCT TGCGGGAGAA TGACTGTCTC TTGATTCACT CCTGTCGGCA ATGGACAACC
    ATCACTGCTC ACAGTTTAGA AGAGGGGCAT TATGTCATTG GTCCAAAGAT TGAGATTCCT
    GTGCATTATG GAGGGCAGTT TAAGCTGCTG GAACAGGACC GGGATATAAA GGAGCCAGTA
    CAGTATTTTA ACAGCGTGGA GGAGGTGGCA AAAGCATTTC CTGAGCGAGT ATACGTCATG
    GAGGAAATAA CATTTAATGT TAAGGTAGCT TCAGGAGAGT GCAATGAAGA TACTGAGGTT
    TACAATATTA CGCTGTGCAC TGGGGATGAG CTAACATTAA TGGGGCAGGC AGAAATTCTT
    TATGCAAAAA CATCCAAGGA GAAGTCCCGA CTCAATACCA TTTTTAAGAA GATTGGAAAG
    CTGAACTCGA TCAGCAAACT GGGCAAAGGC AAAATGCCCT GCCTCATATG CATGAACCAT
    AGAACGAACG AAAGCATCAG CCTCCCGTTT CAGTGTAAGG GAAGGTTCAG CACTCGCAGC
    CCTCTGGAGA TTCAGATGCA GGAAGGAGAA CACACGATAC GCAACATTGT GGAGAAAACA
    AGATTGCCCG TAAATGTAAC TGTGCCTAGC CCACCCCCTA GAAATCCCTA TGACCTTCAT
    TTCATACGGG AAGGGCATCG GTACAAATTT GTCAACATCC AGACCAAGAC TGTTGTAGTT
    TGCTGTGTGT TACAGGGCAA TAAAATAATC CCCATGCATT TTCCATTGCA TTTATCAGTC
    CCAAAGTTTA GCCTTCCAGA AAATCTCGTC AAGGGAGAAG TGTGGCAAGA CTCTGTAGTG
    CAGCACTGGT ACAACACATG CCAAGAACAA TTTGATATAG AAGAATACTC TCGGGCAGTG
    AGGGATGTGA AAGCAGACTG GAGTGAAGAG TGCAAAAGCC CTAAAAAGAC ATGGTGTTCT
    GGCCACAGCC ATATACCAAA TTCCCTCAGT TACGCTCGGG ATGAACTGAC TCAGTCATTC
    CATAGGCTTT CAGTTTGTGT CTATGGAAAC AATCTTCACG GAAACAGTGA GGTGAATCTG
    CATGGCTGCA GAGATCTTTG CGGGGATTGG GCCGTTTTTC CTCAAGATTC CCCACAGTAT
    CAGGACTCGT GTGATAGTGG AAGTGATTAC CTTTTCCCTG AAAGCAGTGA AGAACTAACA
    AGTCTACCAG CCAAGCCAGA ACTTCCTTAT GAGGAGTTAT GGCTAGAACA AGGATCAGTT
    AAAAGGAGTG CGCAGCCTCT CACACGGTCA CAAAGTGAGA AAAACAAATG TGATCCATTC
    AGAGGTTCAC TTCCCTCTCG GTGCGGCACG TCATCTCTTC CCTCTCCTGG ATCTCTTCTG
    ACAACTAAGA GTTTGGATGT ATCCCTACCT CCACCTCCAG TGCCTCCAAA ATCTGAAGCT
    GTAAAAGAAG AATGCAGACT TCTGAATGCT CCGCCTGTGC CTCCACGCAG TTCCAAGCCA
    TCATCCCCCA CTCCATCTGT TTCACCAGCG GCTGGAAAAA TTGCACGGCA GCAAACAAGA
    TCCCCCAGCC CTACCCTTTC TTACTATTCA TCAGGTCTGC ATAGCATAGG CATCACGGAA
    AGTGAAACGA CCAACTCGTC AGATAATTCT CAAGTTTCCT GCTATCCATG TAACTGGATA
    AAAAACGAAA GCGTTGGTCT TGAAAGCGAT TTAACTTGTG GTAGCACATC GTCAGAAGCC
    CTGCCTTCCC GATTATCCTG GCCAAACCGA TATTGTGGTG GGGCAGAAGG CCTTAACGTG
    AATGATTTAC CTATGGATCA GTGTCGCAGT TATTATAGTT ACCCTCGACA AAAGACACCA
    GGAACACCAA AGAAAAATTG TCCAGCTCCA TTTGACTTTG GTGGCTGTGG TTCTTTTACT
    GGGTGCAACC AGGTGGCACA AGGTGACCTC TCAGACATTG TCCCAGGCTG TCCAAAGTCT
    GCAAGCTACT CACTAGAAAG TTCCACTGAT AGAATGATAT CTGACAATAG CACGAAACAA
    AGCTTGTCAT GTCCTGCCTT ACCACCTCGT GCACCAAAAT CCAGTGAAGG CAATGCTCTC
    TTGGAACCCT TGTCTCTGCC TATGAAGATT GATGGAGCCG AAGAGGAAAT TCAGACTTGC
    TCTCCAGACT TTTCAGAAGA ACATTATCTT GCTAAAAAGG GTGTTCAGGA TATTTTCTCT
    ATTTCCTACC CATTCTCCTC ACCACTTCAC ATGCAGCTAG CTCCTAGGTC TTGCGGGGAT
    GGGTCTCCTT GGCAACCACC AACTGATCTT TCAGGACTCT CGGTTGAAGA GGTGTCAAAA
    TCCCTTCGAT TCATTGGTTT GTCAGAGGAC GTGGTGTCAT TTTTTGTTTC GGAAAAAATT
    GATGGAAATT TACTTGTCCA GCTAACAGAA GAAATACTCT CAGAGGACTT TAAGTTAAGC
    AAGTTACAGG TGAAAAAATT ATTACAATTT ATTAACGGCT GGCGACCGAA GATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60004
    Clone ID Related Accession (Same CDS sequence) XM_031903647.1
    Accession Version XM_031903647.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2595bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GRB2-associated and regulator of MAPK protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTTGACAA GCGAGATTAT CAGCAAGACA GCGGTGTCAC CCCTAGAGGA TAGAGCTTTA 
    CTCTATGCAG GAGAAAACAT TGAAGGCTTG CGGGAGAATG ACTGTCTCTT GATTCACTCC
    TGTCGGCAAT GGACAACCAT CACTGCTCAC AGTTTAGAAG AGGGGCATTA TGTCATTGGT
    CCAAAGATTG AGATTCCTGT GCATTATGGA GGGCAGTTTA AGCTGCTGGA ACAGGACCGG
    GATATAAAGG AGCCAGTACA GTATTTTAAC AGCGTGGAGG AGGTGGCAAA AGCATTTCCT
    GAGCGAGTAT ACGTCATGGA GGAAATAACA TTTAATGTTA AGGTAGCTTC AGGAGAGTGC
    AATGAAGATA CTGAGGTTTA CAATATTACG CTGTGCACTG GGGATGAGCT AACATTAATG
    GGGCAGGCAG AAATTCTTTA TGCAAAAACA TCCAAGGAGA AGTCCCGACT CAATACCATT
    TTTAAGAAGA TTGGAAAGCT GAACTCGATC AGCAAACTGG GCAAAGGCAA AATGCCCTGC
    CTCATATGCA TGAACCATAG AACGAACGAA AGCATCAGCC TCCCGTTTCA GTGTAAGGGA
    AGGTTCAGCA CTCGCAGCCC TCTGGAGATT CAGATGCAGG AAGGAGAACA CACGATACGC
    AACATTGTGG AGAAAACAAG ATTGCCCGTA AATGTAACTG TGCCTAGCCC ACCCCCTAGA
    AATCCCTATG ACCTTCATTT CATACGGGAA GGGCATCGGT ACAAATTTGT CAACATCCAG
    ACCAAGACTG TTGTAGTTTG CTGTGTGTTA CAGGGCAATA AAATAATCCC CATGCATTTT
    CCATTGCATT TATCAGTCCC AAAGTTTAGC CTTCCAGAAA ATCTCGTCAA GGGAGAAGTG
    TGGCAAGACT CTGTAGTGCA GCACTGGTAC AACACATGCC AAGAACAATT TGATATAGAA
    GAATACTCTC GGGCAGTGAG GGATGTGAAA GCAGACTGGA GTGAAGAGTG CAAAAGCCCT
    AAAAAGACAT GGTGTTCTGG CCACAGCCAT ATACCAAATT CCCTCAGTTA CGCTCGGGAT
    GAACTGACTC AGTCATTCCA TAGGCTTTCA GTTTGTGTCT ATGGAAACAA TCTTCACGGA
    AACAGTGAGG TGAATCTGCA TGGCTGCAGA GATCTTTGCG GGGATTGGGC CGTTTTTCCT
    CAAGATTCCC CACAGTATCA GGACTCGTGT GATAGTGGAA GTGATTACCT TTTCCCTGAA
    AGCAGTGAAG AACTAACAAG TCTACCAGCC AAGCCAGAAC TTCCTTATGA GGAGTTATGG
    CTAGAACAAG GATCAGTTAA AAGGAGTGCG CAGCCTCTCA CACGGTCACA AAGTGAGAAA
    AACAAATGTG ATCCATTCAG AGGTTCACTT CCCTCTCGGT GCGGCACGTC ATCTCTTCCC
    TCTCCTGGAT CTCTTCTGAC AACTAAGAGT TTGGATGTAT CCCTACCTCC ACCTCCAGTG
    CCTCCAAAAT CTGAAGCTGT AAAAGAAGAA TGCAGACTTC TGAATGCTCC GCCTGTGCCT
    CCACGCAGTT CCAAGCCATC ATCCCCCACT CCATCTGTTT CACCAGCGGC TGGAAAAATT
    GCACGGCAGC AAACAAGATC CCCCAGCCCT ACCCTTTCTT ACTATTCATC AGGTCTGCAT
    AGCATAGGCA TCACGGAAAG TGAAACGACC AACTCGTCAG ATAATTCTCA AGTTTCCTGC
    TATCCATGTA ACTGGATAAA AAACGAAAGC GTTGGTCTTG AAAGCGATTT AACTTGTGGT
    AGCACATCGT CAGAAGCCCT GCCTTCCCGA TTATCCTGGC CAAACCGATA TTGTGGTGGG
    GCAGAAGGCC TTAACGTGAA TGATTTACCT ATGGATCAGT GTCGCAGTTA TTATAGTTAC
    CCTCGACAAA AGACACCAGG AACACCAAAG AAAAATTGTC CAGCTCCATT TGACTTTGGT
    GGCTGTGGTT CTTTTACTGG GTGCAACCAG GTGGCACAAG GTGACCTCTC AGACATTGTC
    CCAGGCTGTC CAAAGTCTGC AAGCTACTCA CTAGAAAGTT CCACTGATAG AATGATATCT
    GACAATAGCA CGAAACAAAG CTTGTCATGT CCTGCCTTAC CACCTCGTGC ACCAAAATCC
    AGTGAAGGCA ATGCTCTCTT GGAACCCTTG TCTCTGCCTA TGAAGATTGA TGGAGCCGAA
    GAGGAAATTC AGACTTGCTC TCCAGACTTT TCAGAAGAAC ATTATCTTGC TAAAAAGGGT
    GTTCAGGATA TTTTCTCTAT TTCCTACCCA TTCTCCTCAC CACTTCACAT GCAGCTAGCT
    CCTAGGTCTT GCGGGGATGG GTCTCCTTGG CAACCACCAA CTGATCTTTC AGGACTCTCG
    GTTGAAGAGG TGTCAAAATC CCTTCGATTC ATTGGTTTGT CAGAGGACGT GGTGTCATTT
    TTTGTTTCGG AAAAAATTGA TGGAAATTTA CTTGTCCAGC TAACAGAAGA AATACTCTCA
    GAGGACTTTA AGTTAAGCAA GTTACAGGTG AAAAAATTAT TACAATTTAT TAACGGCTGG
    CGACCGAAGA TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759507.1
    CDS40..2634
    Translation

    Target ORF information:

    RefSeq Version XM_031903647.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GRB2 associated regulator of MAPK1 subtype 1 (garem1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903647.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTTGACAA GCGAGATTAT CAGCAAGACA GCGGTGTCAC CCCTAGAGGA TAGAGCTTTA 
    CTCTATGCAG GAGAAAACAT TGAAGGCTTG CGGGAGAATG ACTGTCTCTT GATTCACTCC
    TGTCGGCAAT GGACAACCAT CACTGCTCAC AGTTTAGAAG AGGGGCATTA TGTCATTGGT
    CCAAAGATTG AGATTCCTGT GCATTATGGA GGGCAGTTTA AGCTGCTGGA ACAGGACCGG
    GATATAAAGG AGCCAGTACA GTATTTTAAC AGCGTGGAGG AGGTGGCAAA AGCATTTCCT
    GAGCGAGTAT ACGTCATGGA GGAAATAACA TTTAATGTTA AGGTAGCTTC AGGAGAGTGC
    AATGAAGATA CTGAGGTTTA CAATATTACG CTGTGCACTG GGGATGAGCT AACATTAATG
    GGGCAGGCAG AAATTCTTTA TGCAAAAACA TCCAAGGAGA AGTCCCGACT CAATACCATT
    TTTAAGAAGA TTGGAAAGCT GAACTCGATC AGCAAACTGG GCAAAGGCAA AATGCCCTGC
    CTCATATGCA TGAACCATAG AACGAACGAA AGCATCAGCC TCCCGTTTCA GTGTAAGGGA
    AGGTTCAGCA CTCGCAGCCC TCTGGAGATT CAGATGCAGG AAGGAGAACA CACGATACGC
    AACATTGTGG AGAAAACAAG ATTGCCCGTA AATGTAACTG TGCCTAGCCC ACCCCCTAGA
    AATCCCTATG ACCTTCATTT CATACGGGAA GGGCATCGGT ACAAATTTGT CAACATCCAG
    ACCAAGACTG TTGTAGTTTG CTGTGTGTTA CAGGGCAATA AAATAATCCC CATGCATTTT
    CCATTGCATT TATCAGTCCC AAAGTTTAGC CTTCCAGAAA ATCTCGTCAA GGGAGAAGTG
    TGGCAAGACT CTGTAGTGCA GCACTGGTAC AACACATGCC AAGAACAATT TGATATAGAA
    GAATACTCTC GGGCAGTGAG GGATGTGAAA GCAGACTGGA GTGAAGAGTG CAAAAGCCCT
    AAAAAGACAT GGTGTTCTGG CCACAGCCAT ATACCAAATT CCCTCAGTTA CGCTCGGGAT
    GAACTGACTC AGTCATTCCA TAGGCTTTCA GTTTGTGTCT ATGGAAACAA TCTTCACGGA
    AACAGTGAGG TGAATCTGCA TGGCTGCAGA GATCTTTGCG GGGATTGGGC CGTTTTTCCT
    CAAGATTCCC CACAGTATCA GGACTCGTGT GATAGTGGAA GTGATTACCT TTTCCCTGAA
    AGCAGTGAAG AACTAACAAG TCTACCAGCC AAGCCAGAAC TTCCTTATGA GGAGTTATGG
    CTAGAACAAG GATCAGTTAA AAGGAGTGCG CAGCCTCTCA CACGGTCACA AAGTGAGAAA
    AACAAATGTG ATCCATTCAG AGGTTCACTT CCCTCTCGGT GCGGCACGTC ATCTCTTCCC
    TCTCCTGGAT CTCTTCTGAC AACTAAGAGT TTGGATGTAT CCCTACCTCC ACCTCCAGTG
    CCTCCAAAAT CTGAAGCTGT AAAAGAAGAA TGCAGACTTC TGAATGCTCC GCCTGTGCCT
    CCACGCAGTT CCAAGCCATC ATCCCCCACT CCATCTGTTT CACCAGCGGC TGGAAAAATT
    GCACGGCAGC AAACAAGATC CCCCAGCCCT ACCCTTTCTT ACTATTCATC AGGTCTGCAT
    AGCATAGGCA TCACGGAAAG TGAAACGACC AACTCGTCAG ATAATTCTCA AGTTTCCTGC
    TATCCATGTA ACTGGATAAA AAACGAAAGC GTTGGTCTTG AAAGCGATTT AACTTGTGGT
    AGCACATCGT CAGAAGCCCT GCCTTCCCGA TTATCCTGGC CAAACCGATA TTGTGGTGGG
    GCAGAAGGCC TTAACGTGAA TGATTTACCT ATGGATCAGT GTCGCAGTTA TTATAGTTAC
    CCTCGACAAA AGACACCAGG AACACCAAAG AAAAATTGTC CAGCTCCATT TGACTTTGGT
    GGCTGTGGTT CTTTTACTGG GTGCAACCAG GTGGCACAAG GTGACCTCTC AGACATTGTC
    CCAGGCTGTC CAAAGTCTGC AAGCTACTCA CTAGAAAGTT CCACTGATAG AATGATATCT
    GACAATAGCA CGAAACAAAG CTTGTCATGT CCTGCCTTAC CACCTCGTGC ACCAAAATCC
    AGTGAAGGCA ATGCTCTCTT GGAACCCTTG TCTCTGCCTA TGAAGATTGA TGGAGCCGAA
    GAGGAAATTC AGACTTGCTC TCCAGACTTT TCAGAAGAAC ATTATCTTGC TAAAAAGGGT
    GTTCAGGATA TTTTCTCTAT TTCCTACCCA TTCTCCTCAC CACTTCACAT GCAGCTAGCT
    CCTAGGTCTT GCGGGGATGG GTCTCCTTGG CAACCACCAA CTGATCTTTC AGGACTCTCG
    GTTGAAGAGG TGTCAAAATC CCTTCGATTC ATTGGTTTGT CAGAGGACGT GGTGTCATTT
    TTTGTTTCGG AAAAAATTGA TGGAAATTTA CTTGTCCAGC TAACAGAAGA AATACTCTCA
    GAGGACTTTA AGTTAAGCAA GTTACAGGTG AAAAAATTAT TACAATTTAT TAACGGCTGG
    CGACCGAAGA TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60005
    Clone ID Related Accession (Same CDS sequence) XM_031903648.1
    Accession Version XM_031903648.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GRB2-associated and regulator of MAPK protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGGCAGG CAGAAATTCT TTATGCAAAA ACATCCAAGG AGAAGTCCCG ACTCAATACC 
    ATTTTTAAGA AGATTGGAAA GCTGAACTCG ATCAGCAAAC TGGGCAAAGG CAAAATGCCC
    TGCCTCATAT GCATGAACCA TAGAACGAAC GAAAGCATCA GCCTCCCGTT TCAGTGTAAG
    GGAAGGTTCA GCACTCGCAG CCCTCTGGAG ATTCAGATGC AGGAAGGAGA ACACACGATA
    CGCAACATTG TGGAGAAAAC AAGATTGCCC GTAAATGTAA CTGTGCCTAG CCCACCCCCT
    AGAAATCCCT ATGACCTTCA TTTCATACGG GAAGGGCATC GGTACAAATT TGTCAACATC
    CAGACCAAGA CTGTTGTAGT TTGCTGTGTG TTACAGGGCA ATAAAATAAT CCCCATGCAT
    TTTCCATTGC ATTTATCAGT CCCAAAGTTT AGCCTTCCAG AAAATCTCGT CAAGGGAGAA
    GTGTGGCAAG ACTCTGTAGT GCAGCACTGG TACAACACAT GCCAAGAACA ATTTGATATA
    GAAGAATACT CTCGGGCAGT GAGGGATGTG AAAGCAGACT GGAGTGAAGA GTGCAAAAGC
    CCTAAAAAGA CATGGTGTTC TGGCCACAGC CATATACCAA ATTCCCTCAG TTACGCTCGG
    GATGAACTGA CTCAGTCATT CCATAGGCTT TCAGTTTGTG TCTATGGAAA CAATCTTCAC
    GGAAACAGTG AGGTGAATCT GCATGGCTGC AGAGATCTTT GCGGGGATTG GGCCGTTTTT
    CCTCAAGATT CCCCACAGTA TCAGGACTCG TGTGATAGTG GAAGTGATTA CCTTTTCCCT
    GAAAGCAGTG AAGAACTAAC AAGTCTACCA GCCAAGCCAG AACTTCCTTA TGAGGAGTTA
    TGGCTAGAAC AAGGATCAGT TAAAAGGAGT GCGCAGCCTC TCACACGGTC ACAAAGTGAG
    AAAAACAAAT GTGATCCATT CAGAGGTTCA CTTCCCTCTC GGTGCGGCAC GTCATCTCTT
    CCCTCTCCTG GATCTCTTCT GACAACTAAG AGTTTGGATG TATCCCTACC TCCACCTCCA
    GTGCCTCCAA AATCTGAAGC TGTAAAAGAA GAATGCAGAC TTCTGAATGC TCCGCCTGTG
    CCTCCACGCA GTTCCAAGCC ATCATCCCCC ACTCCATCTG TTTCACCAGC GGCTGGAAAA
    ATTGCACGGC AGCAAACAAG ATCCCCCAGC CCTACCCTTT CTTACTATTC ATCAGGTCTG
    CATAGCATAG GCATCACGGA AAGTGAAACG ACCAACTCGT CAGATAATTC TCAAGTTTCC
    TGCTATCCAT GTAACTGGAT AAAAAACGAA AGCGTTGGTC TTGAAAGCGA TTTAACTTGT
    GGTAGCACAT CGTCAGAAGC CCTGCCTTCC CGATTATCCT GGCCAAACCG ATATTGTGGT
    GGGGCAGAAG GCCTTAACGT GAATGATTTA CCTATGGATC AGTGTCGCAG TTATTATAGT
    TACCCTCGAC AAAAGACACC AGGAACACCA AAGAAAAATT GTCCAGCTCC ATTTGACTTT
    GGTGGCTGTG GTTCTTTTAC TGGGTGCAAC CAGGTGGCAC AAGGTGACCT CTCAGACATT
    GTCCCAGGCT GTCCAAAGTC TGCAAGCTAC TCACTAGAAA GTTCCACTGA TAGAATGATA
    TCTGACAATA GCACGAAACA AAGCTTGTCA TGTCCTGCCT TACCACCTCG TGCACCAAAA
    TCCAGTGAAG GCAATGCTCT CTTGGAACCC TTGTCTCTGC CTATGAAGAT TGATGGAGCC
    GAAGAGGAAA TTCAGACTTG CTCTCCAGAC TTTTCAGAAG AACATTATCT TGCTAAAAAG
    GGTGTTCAGG ATATTTTCTC TATTTCCTAC CCATTCTCCT CACCACTTCA CATGCAGCTA
    GCTCCTAGGT CTTGCGGGGA TGGGTCTCCT TGGCAACCAC CAACTGATCT TTCAGGACTC
    TCGGTTGAAG AGGTGTCAAA ATCCCTTCGA TTCATTGGTT TGTCAGAGGA CGTGGTGTCA
    TTTTTTGTTT CGGAAAAAAT TGATGGAAAT TTACTTGTCC AGCTAACAGA AGAAATACTC
    TCAGAGGACT TTAAGTTAAG CAAGTTACAG GTGAAAAAAT TATTACAATT TATTAACGGC
    TGGCGACCGA AGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759508.1
    CDS159..2336
    Translation

    Target ORF information:

    RefSeq Version XM_031903648.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GRB2 associated regulator of MAPK1 subtype 1 (garem1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903648.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGGCAGG CAGAAATTCT TTATGCAAAA ACATCCAAGG AGAAGTCCCG ACTCAATACC 
    ATTTTTAAGA AGATTGGAAA GCTGAACTCG ATCAGCAAAC TGGGCAAAGG CAAAATGCCC
    TGCCTCATAT GCATGAACCA TAGAACGAAC GAAAGCATCA GCCTCCCGTT TCAGTGTAAG
    GGAAGGTTCA GCACTCGCAG CCCTCTGGAG ATTCAGATGC AGGAAGGAGA ACACACGATA
    CGCAACATTG TGGAGAAAAC AAGATTGCCC GTAAATGTAA CTGTGCCTAG CCCACCCCCT
    AGAAATCCCT ATGACCTTCA TTTCATACGG GAAGGGCATC GGTACAAATT TGTCAACATC
    CAGACCAAGA CTGTTGTAGT TTGCTGTGTG TTACAGGGCA ATAAAATAAT CCCCATGCAT
    TTTCCATTGC ATTTATCAGT CCCAAAGTTT AGCCTTCCAG AAAATCTCGT CAAGGGAGAA
    GTGTGGCAAG ACTCTGTAGT GCAGCACTGG TACAACACAT GCCAAGAACA ATTTGATATA
    GAAGAATACT CTCGGGCAGT GAGGGATGTG AAAGCAGACT GGAGTGAAGA GTGCAAAAGC
    CCTAAAAAGA CATGGTGTTC TGGCCACAGC CATATACCAA ATTCCCTCAG TTACGCTCGG
    GATGAACTGA CTCAGTCATT CCATAGGCTT TCAGTTTGTG TCTATGGAAA CAATCTTCAC
    GGAAACAGTG AGGTGAATCT GCATGGCTGC AGAGATCTTT GCGGGGATTG GGCCGTTTTT
    CCTCAAGATT CCCCACAGTA TCAGGACTCG TGTGATAGTG GAAGTGATTA CCTTTTCCCT
    GAAAGCAGTG AAGAACTAAC AAGTCTACCA GCCAAGCCAG AACTTCCTTA TGAGGAGTTA
    TGGCTAGAAC AAGGATCAGT TAAAAGGAGT GCGCAGCCTC TCACACGGTC ACAAAGTGAG
    AAAAACAAAT GTGATCCATT CAGAGGTTCA CTTCCCTCTC GGTGCGGCAC GTCATCTCTT
    CCCTCTCCTG GATCTCTTCT GACAACTAAG AGTTTGGATG TATCCCTACC TCCACCTCCA
    GTGCCTCCAA AATCTGAAGC TGTAAAAGAA GAATGCAGAC TTCTGAATGC TCCGCCTGTG
    CCTCCACGCA GTTCCAAGCC ATCATCCCCC ACTCCATCTG TTTCACCAGC GGCTGGAAAA
    ATTGCACGGC AGCAAACAAG ATCCCCCAGC CCTACCCTTT CTTACTATTC ATCAGGTCTG
    CATAGCATAG GCATCACGGA AAGTGAAACG ACCAACTCGT CAGATAATTC TCAAGTTTCC
    TGCTATCCAT GTAACTGGAT AAAAAACGAA AGCGTTGGTC TTGAAAGCGA TTTAACTTGT
    GGTAGCACAT CGTCAGAAGC CCTGCCTTCC CGATTATCCT GGCCAAACCG ATATTGTGGT
    GGGGCAGAAG GCCTTAACGT GAATGATTTA CCTATGGATC AGTGTCGCAG TTATTATAGT
    TACCCTCGAC AAAAGACACC AGGAACACCA AAGAAAAATT GTCCAGCTCC ATTTGACTTT
    GGTGGCTGTG GTTCTTTTAC TGGGTGCAAC CAGGTGGCAC AAGGTGACCT CTCAGACATT
    GTCCCAGGCT GTCCAAAGTC TGCAAGCTAC TCACTAGAAA GTTCCACTGA TAGAATGATA
    TCTGACAATA GCACGAAACA AAGCTTGTCA TGTCCTGCCT TACCACCTCG TGCACCAAAA
    TCCAGTGAAG GCAATGCTCT CTTGGAACCC TTGTCTCTGC CTATGAAGAT TGATGGAGCC
    GAAGAGGAAA TTCAGACTTG CTCTCCAGAC TTTTCAGAAG AACATTATCT TGCTAAAAAG
    GGTGTTCAGG ATATTTTCTC TATTTCCTAC CCATTCTCCT CACCACTTCA CATGCAGCTA
    GCTCCTAGGT CTTGCGGGGA TGGGTCTCCT TGGCAACCAC CAACTGATCT TTCAGGACTC
    TCGGTTGAAG AGGTGTCAAA ATCCCTTCGA TTCATTGGTT TGTCAGAGGA CGTGGTGTCA
    TTTTTTGTTT CGGAAAAAAT TGATGGAAAT TTACTTGTCC AGCTAACAGA AGAAATACTC
    TCAGAGGACT TTAAGTTAAG CAAGTTACAG GTGAAAAAAT TATTACAATT TATTAACGGC
    TGGCGACCGA AGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.