ARHGAP20 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGAP20
Entrez Gene ID	57569
Full Name	Rho GTPase activating protein 20
Synonyms	RARHOGAP
General protein information	Preferred Names Rho GTPase activating protein 20 Names rho GTPase-activating protein 20 RA and RhoGAP domain containing protein rho GTPase activating protein 20 variant 2 rho-type GTPase-activating protein 20
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q22.3-q23.1
Summary	The protein encoded by this gene is an activator of RHO-type GTPases, transducing a signal from RAP1 to RHO and impacting neurite outgrowth. [provided by RefSeq, Sep 2016].
Disorder MIM:	609568

mRNA and Protein(s)

mRNA	Protein	Name
NM_001258416.1	NP_001245345.1	rho GTPase-activating protein 20 isoform 3
NM_001258415.2	NP_001245344.1	rho GTPase-activating protein 20 isoform 2
XM_006718893.3	XP_006718956.1	rho GTPase-activating protein 20 isoform X1
XM_005271628.3	XP_005271685.1	rho GTPase-activating protein 20 isoform X2
NM_001258415.1	NP_001245344.1	rho GTPase-activating protein 20 isoform 2
NM_001258418.1	NP_001245347.1	rho GTPase-activating protein 20 isoform 4
NM_020809.3	NP_065860.2	rho GTPase-activating protein 20 isoform 1
NM_001258417.1	NP_001245346.1	rho GTPase-activating protein 20 isoform 4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARHGAP20	XP_003313436.1
Mus musculus (house mouse)	Arhgap20	NP_780744.2
Gallus gallus (chicken)	ARHGAP20	XP_417154.2
Homo sapiens (human)	ARHGAP20	NP_065860.2
Bos taurus (cattle)	ARHGAP20	NP_001193662.1
	ARHGAP20	XP_001098860.2
Canis lupus familiaris (dog)	ARHGAP20	XP_005619862.1
Rattus norvegicus (Norway rat)	Arhgap20	NP_998794.1

Gene Ontology
Bibliography

Related articles in PubMed

Heritability and genome-wide association analysis of renal sinus fat accumulation in the Framingham Heart Study.
Foster MC, Yang Q, Hwang SJ, Hoffmann U, Fox CS
BMC medical genetics12148(2011 Nov)

Expression analysis of genes located in the minimally deleted regions of 13q14 and 11q22-23 in chronic lymphocytic leukemia-unexpected expression pattern of the RHO GTPase activator ARHGAP20.
Herold T, Jurinovic V, Mulaw M, Seiler T, Dufour A, Schneider S, Kakadia PM, Feuring-Buske M, Braess J, Spiekermann K, Mansmann U, Hiddemann W, Buske C, Bohlander SK
Genes, chromosomes & cancer50(7)546-58(2011 Jul)

RA-RhoGAP, Rap-activated Rho GTPase-activating protein implicated in neurite outgrowth through Rho.
Yamada T, Sakisaka T, Hisata S, Baba T, Takai Y
The Journal of biological chemistry280(38)33026-34(2005 Sep)

Translocation t(X;11)(q13;q23) in B-cell chronic lymphocytic leukemia disrupts two novel genes.
Kalla C, Nentwich H, Schlotter M, Mertens D, Wildenberger K, D?hner H, Stilgenbauer S, Lichter P
Genes, chromosomes & cancer42(2)128-43(2005 Feb)

Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male germ cells.
Curry BJ, Su H, Law EG, McLaughlin EA, Nixon B, Aitken RJ
Genomics84(2)406-18(2004 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The similar expression profiles of ARHGAP20 in 13q14 and 11q22-23 deleted CLL cases suggest a molecular connection and an intriguing mechanism of regulation
Title: Expression analysis of genes located in the minimally deleted regions of 13q14 and 11q22-23 in chronic lymphocytic leukemia-unexpected expression pattern of the RHO GTPase activator ARHGAP20.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

RA-RhoGAP transduces a signal from Rap1 to Rho and regulates the neurite outgrowth
Title: RA-RhoGAP, Rap-activated Rho GTPase-activating protein implicated in neurite outgrowth through Rho.

human KIAA1391 gene and mouse Kiaa1391 gene encode RhoGAP proteins with RA domain and two ANXL repeats
Title: Identification and characterization of human KIAA1391 and mouse Kiaa1391 genes encoding novel RhoGAP family proteins with RA domain and ANXL repeats.

The following ARHGAP20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGAP20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu45843	XM_005271628.3 Latest version!	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu08015	NM_001258416.1 Latest version!	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu08022	NM_001258418.1 Latest version!	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu08022	NM_001258417.1 Latest version!	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu08682	NM_020809.3 Latest version!	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu12330	NM_001258415.2 Latest version!	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu08682	XM_006718893.3 Latest version!	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu12330	NM_001258415.1	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGAP20 ( XM_005271628.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005271685.1 Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu45843
Clone ID Related Accession (Same CDS sequence)	XM_005271628.3
Accession Version	XM_005271628.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005271628.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAAG GAAATGATGA AGAGAAAATA AATACTGTTC AAAGGCTATT AGACCAGCTT 
CCGAGAGCCA ATGTTGTTCT CCTAAGGTAT CTTTTTGGGG TGTTACACAA CATTGAGCAA 
CATTCCTCAT CCAATCAGAT GACTGCATTT AATTTAGCTG TGTGTGTCGC TCCAAGTATT 
CTTTGGCCTC CTGCTTCCTC CAGCCCAGAA CTAGAAAACG AATTTACAAA AAAGGTTTCC 
CTGCTTATAC AATTTCTGAT TGAGAATTGC CTTAGGATAT TTGGAGAAGA AATCACTTCC 
CTCTTCAGAG AGGTTTCAGT GAGATGTGAC ACTAGAGAGA ATGCCTCAGA TATTTCTTGC 
TTTCAACTGA ATGACTCCTC CTATGACAGC TTGGAAAATG AGCTAAATGA GGATGTTGAT 
GCACCATGCA GTGACTTGGT AAAGAAACTT GGCCAGGGGA GCAGAAGCAT GGACTCTGTC 
TTAACCCTCA GTGACTATGA TCTTGACCAG CCCGAGGTGG AAGGCCTTTT AACCCTAAGC 
GACTTTGACT TGGCCCATTC TAAAGATGAA GATGTTCAAA TGAAACGGCC TCTTGAATCC 
AAGCCGGTGA ACATTTTAGT GTACACAAAG ATCCCACTGC GGGATCATGC CAGGGCCCCA 
TCTGCCATGT GCACACCCAG CTACCTGTCC ACAGCTGCAG CAAATGCTGC AAAAAGCCTG 
AGGCGACACC GGCGTTGCTC AGAGCCCAGC ATCGACTATC TGGATTCAAA GCTTTCCTAC 
CTCAGGGAGT TTTATCAGAA AAAGCTACGC AAGTCCAGCT GTGATGCAAT TCTTTCTCAA 
AAAGATGAAG ACTATCTGAA GCAGAATCAA CCCCTCCAGG AGGAAGGAAA GACATGTTTT 
AAACAGAGTT TAGTCACAGG CACTGATGTC AGCAAGAAAA ATGCCACTAC TCAAAACACT 
AAGAAGAAAA GCTTGTCTGG TAGTGAAGGA AATCACGTGA AACTTTTCCC TAAGTCTAAG 
CCAGTGGCCA TTTCTGTGGC ATCTTATAGT CCTATGTCCT CACAGGATCA TTCCAAGAAC 
CAGCCCTTTG ATGTGAATAC ATCTGGATAC TCCCCACCAC ACACAGCAGA TGCCCTCAAG 
GGTCCAAGGA CACATCGGCG CTGCTCAGAG CCCAACATAG AAGACCAGAA CCGCAAGCTG 
ACCTATCTCA GGGGAATTTA TTCAAAGAAA CAACATAAAA CCAGCTGTGA AGCTGGTCTC 
TTGCATGGAG AGGAGGATTA TCTCAAACGG CATAAGTCTT TGCAAATGGA GGGGCAGAAG 
CTCATTAATC AGAGTTTAGT CATGGGGATT GAGGTGGGCA AGAGTAGTGC CACAAACCAA 
AACACTGAGA AGGTTTTACC CCCAAGATTA AACCTTTGCC CAAGGACCAG CTATTCCAGC 
TTATCCTCCC CAGGCACTTC CCCATCCGGC TCATCAGTAA GCTCCCAAGA CAGTGCTTTT 
TCTCAGATTT CTGAACACTC TGTGTTTACA CCCACTGAGA CTTCCTCTCC AATAGATTGC 
ACTTTTCAGG CTCAGAGAAA ACGGGAAGAC CTTTCTCCTG ACTTTAGCAA TGCCAGCCAT 
GTTTCCGGAA TGCCCGGTCC CTCATCAGGG CAGGCTTGCA GCCGCCCAGC CTATACAAAG 
AAGGACACCA TGGAGTGGCA TTCACAAATG CATTCTGTAA CTCTTCATCC CAGCACATGG 
TTGAGAAATG GTGTGGCCAG TTTGAAAAAC TGGTCCCTCA AAAAGAAAGC AAAGGCAGCC 
AGACCAGAGG AAGAGAAAAT AGCTTCTCCA AAAGGACCCT TAGAGCCACC CCCACATGCT 
TCTGGTGTTC CAGAAGCCAA CTCACTGCAA GAGGAACAAA AAGACTTGCC CTTAAGGGCA 
GCTGAAGGAC TGTCCCCTGT GCAGTCAGCC CAAAGGTGTA GTTCTTCTCC CTTCCAGGAC 
TCAGAGAGAC ACTGTAGCTC TCCATTCAGC CTGGTGGAGA GCAGACTTAA GCTGTGCATG 
AAGTCACATG AGGAAATAGA GCCTGGTAGT CAGAGCTCTT CTGGTTCTCT GCCTTGGGAA 
AGAGCCTCAG CCAGCTCTTG GACTCTAGAG GATGCGACCA GCCCAGACTC AGGGCCTACA 
GTGGTCTGCG ACATTGAGGA CAGGTATTTA ACCAAAGACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005271685.1
CDS	331..2535
Translation	MDQGNDEEKINTVQRLLDQLPRANVVLLRYLFGVLHNIEQHSSSNQMTAFNLAVCVAPSILWPPASSSPELENEFTKKVSLLIQFLIENCLRIFGEEITSLFREVSVRCDTRENASDISCFQLNDSSYDSLENELNEDVDAPCSDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLAHSKDEDVQMKRPLESKPVNILVYTKIPLRDHARAPSAMCTPSYLSTAAANAAKSLRRHRRCSEPSIDYLDSKLSYLREFYQKKLRKSSCDAILSQKDEDYLKQNQPLQEEGKTCFKQSLVTGTDVSKKNATTQNTKKKSLSGSEGNHVKLFPKSKPVAISVASYSPMSSQDHSKNQPFDVNTSGYSPPHTADALKGPRTHRRCSEPNIEDQNRKLTYLRGIYSKKQHKTSCEAGLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNTEKVLPPRLNLCPRTSYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQAQRKREDLSPDFSNASHVSGMPGPSSGQACSRPAYTKKDTMEWHSQMHSVTLHPSTWLRNGVASLKNWSLKKKAKAARPEEEKIASPKGPLEPPPHASGVPEANSLQEEQKDLPLRAAEGLSPVQSAQRCSSSPFQDSERHCSSPFSLVESRLKLCMKSHEEIEPGSQSSSGSLPWERASASSWTLEDATSPDSGPTVVCDIEDRYLTKDI

Target ORF information:

RefSeq Version	XM_005271628.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271628.3

ORF Insert Sequence:

ATGGATCAAG GAAATGATGA AGAGAAAATA AATACTGTTC AAAGGCTATT AGACCAGCTT 
CCGAGAGCCA ATGTTGTTCT CCTAAGGTAT CTTTTTGGGG TGTTACACAA CATTGAGCAA 
CATTCCTCAT CCAATCAGAT GACTGCATTT AATTTAGCTG TGTGTGTCGC TCCAAGTATT 
CTTTGGCCTC CTGCTTCCTC CAGCCCAGAA CTAGAAAACG AATTTACAAA AAAGGTTTCC 
CTGCTTATAC AATTTCTGAT TGAGAATTGC CTTAGGATAT TTGGAGAAGA AATCACTTCC 
CTCTTCAGAG AGGTTTCAGT GAGATGTGAC ACTAGAGAGA ATGCCTCAGA TATTTCTTGC 
TTTCAACTGA ATGACTCCTC CTATGACAGC TTGGAAAATG AGCTAAATGA GGATGTTGAT 
GCACCATGCA GTGACTTGGT AAAGAAACTT GGCCAGGGGA GCAGAAGCAT GGACTCTGTC 
TTAACCCTCA GTGACTATGA TCTTGACCAG CCCGAGGTGG AAGGCCTTTT AACCCTAAGC 
GACTTTGACT TGGCCCATTC TAAAGATGAA GATGTTCAAA TGAAACGGCC TCTTGAATCC 
AAGCCGGTGA ACATTTTAGT GTACACAAAG ATCCCACTGC GGGATCATGC CAGGGCCCCA 
TCTGCCATGT GCACACCCAG CTACCTGTCC ACAGCTGCAG CAAATGCTGC AAAAAGCCTG 
AGGCGACACC GGCGTTGCTC AGAGCCCAGC ATCGACTATC TGGATTCAAA GCTTTCCTAC 
CTCAGGGAGT TTTATCAGAA AAAGCTACGC AAGTCCAGCT GTGATGCAAT TCTTTCTCAA 
AAAGATGAAG ACTATCTGAA GCAGAATCAA CCCCTCCAGG AGGAAGGAAA GACATGTTTT 
AAACAGAGTT TAGTCACAGG CACTGATGTC AGCAAGAAAA ATGCCACTAC TCAAAACACT 
AAGAAGAAAA GCTTGTCTGG TAGTGAAGGA AATCACGTGA AACTTTTCCC TAAGTCTAAG 
CCAGTGGCCA TTTCTGTGGC ATCTTATAGT CCTATGTCCT CACAGGATCA TTCCAAGAAC 
CAGCCCTTTG ATGTGAATAC ATCTGGATAC TCCCCACCAC ACACAGCAGA TGCCCTCAAG 
GGTCCAAGGA CACATCGGCG CTGCTCAGAG CCCAACATAG AAGACCAGAA CCGCAAGCTG 
ACCTATCTCA GGGGAATTTA TTCAAAGAAA CAACATAAAA CCAGCTGTGA AGCTGGTCTC 
TTGCATGGAG AGGAGGATTA TCTCAAACGG CATAAGTCTT TGCAAATGGA GGGGCAGAAG 
CTCATTAATC AGAGTTTAGT CATGGGGATT GAGGTGGGCA AGAGTAGTGC CACAAACCAA 
AACACTGAGA AGGTTTTACC CCCAAGATTA AACCTTTGCC CAAGGACCAG CTATTCCAGC 
TTATCCTCCC CAGGCACTTC CCCATCCGGC TCATCAGTAA GCTCCCAAGA CAGTGCTTTT 
TCTCAGATTT CTGAACACTC TGTGTTTACA CCCACTGAGA CTTCCTCTCC AATAGATTGC 
ACTTTTCAGG CTCAGAGAAA ACGGGAAGAC CTTTCTCCTG ACTTTAGCAA TGCCAGCCAT 
GTTTCCGGAA TGCCCGGTCC CTCATCAGGG CAGGCTTGCA GCCGCCCAGC CTATACAAAG 
AAGGACACCA TGGAGTGGCA TTCACAAATG CATTCTGTAA CTCTTCATCC CAGCACATGG 
TTGAGAAATG GTGTGGCCAG TTTGAAAAAC TGGTCCCTCA AAAAGAAAGC AAAGGCAGCC 
AGACCAGAGG AAGAGAAAAT AGCTTCTCCA AAAGGACCCT TAGAGCCACC CCCACATGCT 
TCTGGTGTTC CAGAAGCCAA CTCACTGCAA GAGGAACAAA AAGACTTGCC CTTAAGGGCA 
GCTGAAGGAC TGTCCCCTGT GCAGTCAGCC CAAAGGTGTA GTTCTTCTCC CTTCCAGGAC 
TCAGAGAGAC ACTGTAGCTC TCCATTCAGC CTGGTGGAGA GCAGACTTAA GCTGTGCATG 
AAGTCACATG AGGAAATAGA GCCTGGTAGT CAGAGCTCTT CTGGTTCTCT GCCTTGGGAA 
AGAGCCTCAG CCAGCTCTTG GACTCTAGAG GATGCGACCA GCCCAGACTC AGGGCCTACA 
GTGGTCTGCG ACATTGAGGA CAGGTATTTA ACCAAAGACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP20 ( NM_001258416.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001245345.1 Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu08015
Clone ID Related Accession (Same CDS sequence)	NM_001258416.1
Accession Version	NM_001258416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 20 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY496267.1 and AP001883.6. Transcript Variant: This variant (3) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY496267.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATTTT GGATTATAAT AAATAAGAAA ATGAAAACAC TAGCAGAAAG GAGGAGGAGC 
GCTCCATCTC TTATCCTGGA TAAAGCCCTA CAAAAACGGC CTACTACCAG GGACAGTCCT 
TCTGCTAGTG TTGACACATG CACATTTCTG TCATCATTAG TGTGCTCCAA TAGGACTCTG 
CTGATTGATG GCCGGGCAGA ACTCAAAAGA GGCCTCCAGA GGCAGGAGCG GCATCTTTTC 
CTATTCAATG ATCTGTTTGT TGTGGCCAAA ATCAAATATA ACAATAACTT TAAGATAAAA 
AATAAAATTA AATTAACTGA TATGTGGACA GCAAGCTGTG TGGATGAAGT GGGAGAAGGC 
AACACCAATG CCATGAAATC CTTTGTTTTG GGCTGGCCCA CAGTGAACTT TGTGGCCACT 
TTCAGTTCTC CAGAACAAAA GGACAAATGG CTCTCTCTCC TTCAGAGATA CATCAATCTA 
GAGAAAGAAA AGGACTACCC GAAGAGCATT CCCCTCAAAA TCTTCGCCAA GGACATTGGG 
AATTGTGCCT ACTCTAAAAC TATAACAGTA ATGAATTCAG ATACAGCGAA TGAAGTTATC 
AACATGTCAT TACCAATGCT AGGGATAACT GGCTCTGAGA GAGATTACCA GTTGTGGGTC 
AATTCTGGCA AAGAAGAGGC TCCATACCCA CTCATTGGGC ATGAATATCC ATATGGAATT 
AAAATGAGCC ATCTTCGAGA CTCTGCACTC CTGACACCGG GATCAAAGGA CTCTACCACC 
CCTTTCAACC TCCAGGAGCC CTTCCTTATG GAACAGCTCC CCCGAGAGAT GCAGTGCCAG 
TTCATCCTGA AGCCCAGCCG CCTGGCTGCA GCCCAGCAAC TGAGTGATTC AGGTCATAAG 
ACATTTAAAA GGAGAAGATC TATCATAAAC TGGGCCTTCT GGCGAGGTTC TAGCACTCAC 
CTGGACAACT TGCCCTCATC GCCAACATCA CCTATGCCAG GACAGCTCTT TGGAATTTCT 
CTGCCAAATA TTTGTGAGAA TGACAATCTG CCCAAACCTG TCTTGGATAT GCTTTTCTTT 
CTTAATCAAA AAGGACCTCT CACCAAAGGT ATCTTCAGGC AATCAGCCAA TGTGAAATCC 
TGCAGAGAAC TAAAAGAGAA ATTGAATTCT GGAGTCGAAG TACACCTAGA CTGTGAATCT 
ATTTTTGTGA TAGCATCTGT CTTAAAGGAT TTTCTGCGAA ATATTCCAGG AAGTATTTTT 
TCATCAGATC TCTATGATCA CTGGGTCTCT GTAATGGATC AAGGAAATGA TGAAGAGAAA 
ATAAATACTG TTCAAAGGCT ATTAGACCAG CTTCCGAGAG CCAATGTTGT TCTCCTAAGG 
TATCTTTTTG GGGTGTTACA CAACATTGAG CAACATTCCT CATCCAATCA GATGACTGCA 
TTTAATTTAG CTGTGTGTGT CGCTCCAAGT ATTCTTTGGC CTCCTGCTTC CTCCAGCCCA 
GAACTAGAAA ACGAATTTAC AAAAAAGGTT TCCCTGCTTA TACAATTTCT GATTGAGAAT 
TGCCTTAGGA TATTTGGAGA AGAAATCACT TCCCTCTTCA GAGAGGTTTC AGTGAGATGT 
GACACTAGAG AGAATGCCTC AGATATTTCT TGCTTTCAAC TGAATGACTC CTCCTATGAC 
AGCTTGGAAA ATGAGCTAAA TGAGGATGTT GATGCACCAT GCAGTGACTT GGTAAAGAAA 
CTTGGCCAGG GGAGCAGAAG CATGGACTCT GTCTTAACCC TCAGTGACTA TGATCTTGAC 
CAGCCCGAGG TGGAAGGCCT TTTAACCCTA AGCGACTTTG ACTTGGCCCA TTCTAAAGAT 
GAAGATGTTC AAATGAAACG GCCTCTTGAA TCCAAGCCGG TGAACATTTT AGTGTACACA 
AAGATCCCAC TGCGGGATCA TGCCAGGGCC CCATCTGCCA TGTGCACACC CAGCTACCTG 
TCCACAGCTG CAGCAAATGC TGCAAAAAGC CTGAGGCGAC ACCGGCGTTG CTCAGAGCCC 
AGCATCGACT ATCTGGATTC AAAGCTTTCC TACCTCAGGG AGTTTTATCA GAAAAAGCTA 
CGCAAGTCCA GCTGTGATGC AATTCTTTCT CAAAAAGATG AAGACTATCT GAAGCAGAAT 
CAACCCCTCC AGGAGGAAGG AAAGACATGT TTTAAACAGA GTTTAGTCAC AGGCACTGAT 
GTCAGCAAGA AAAATGCCAC TACTCAAAAC ACTAAGAAGA AAAGCTTGTC TGGTAGTGAA 
GGAAATCACG TGAAACTTTT CCCTAAGTCT AAGCCAGTGG CCATTTCTGT GGCATCTTAT 
AGTCCTATGT CCTCACAGGA TCATTCCAAG AACCAGCCCT TTGATGTGAA TACATCTGGA 
TACTCCCCAC CACACACAGC AGATGCCCTC AAGGGTCCAA GGACACATCG GCGCTGCTCA 
GAGCCCAACA TAGAAGACCA GAACCGCAAG CTGACCTATC TCAGGGGAAT TTATTCAAAG 
AAACAACATA AAACCAGCTG TGAAGCTGGT CTCTTGCATG GAGAGGAGGA TTATCTCAAA 
CGGCATAAGT CTTTGCAAAT GGAGGGGCAG AAGCTCATTA ATCAGAGTTT AGTCATGGGG 
ATTGAGGTGG GCAAGAGTAG TGCCACAAAC CAAAACACTG AGAAGGTTTT ACCCCCAAGA 
TTAAACCTTT GCCCAAGGAC CAGCTATTCC AGCTTATCCT CCCCAGGCAC TTCCCCATCC 
GGCTCATCAG TAAGCTCCCA AGACAGTGCT TTTTCTCAGA TTTCTGAACA CTCTGTGTTT 
ACACCCACTG AGACTTCCTC TCCAATAGAT TGCACTTTTC AGGCTCAGAG AAAACGGGAA 
GACCTTTCTC CTGACTTTAG CAATGCCAGC CATGTTTCCG GAATGCCCGG TCCCTCATCA 
GGGCAGGCTT GCAGCCGCCC AGCCTATACA AAGAAGGACA CCATGGAGTG GCATTCACAA 
ATGCATTCTG TAACTCTTCA TCCCAGCACA TGGTTGAGAA ATGGTGTGGC CAGTTTGAAA 
AACTGGTCCC TCAAAAAGAA AGCAAAGGCA GCCAGACCAG AGGAAGAGAA AATAGCTTCT 
CCAAAAGGAC CCTTAGAGCC ACCCCCACAT GCTTCTGGTG TTCCAGAAGC CAACTCACTG 
CAAGAGGAAC AAAAAGACTT GCCCTTAAGG GCAGCTGAAG GACTGTCCCC TGTGCAGTCA 
GCCCAAAGGT GTAGTTCTTC TCCCTTCCAG GACTCAGAGA GACACTGTAG CTCTCCATTC 
AGCCTGGTGG AGAGCAGACT TAAGCTGTGC ATGAAGTCAC ATGAGGAAAT AGAGCCTGGT 
AGTCAGAGCT CTTCTGGTTC TCTGCCTTGG GAAAGAGCCT CAGCCAGCTC TTGGACTCTA 
GAGGATGCGA CCAGCCCAGA CTCAGGGCCT ACAGTGGTCT GCGACATTGA GGACAGGTAT 
TTAACCAAAG ACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245345.1
CDS	253..3750
Translation	MTFWIIINKKMKTLAERRRSAPSLILDKALQKRPTTRDSPSASVDTCTFLSSLVCSNRTLLIDGRAELKRGLQRQERHLFLFNDLFVVAKIKYNNNFKIKNKIKLTDMWTASCVDEVGEGNTNAMKSFVLGWPTVNFVATFSSPEQKDKWLSLLQRYINLEKEKDYPKSIPLKIFAKDIGNCAYSKTITVMNSDTANEVINMSLPMLGITGSERDYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDSALLTPGSKDSTTPFNLQEPFLMEQLPREMQCQFILKPSRLAAAQQLSDSGHKTFKRRRSIINWAFWRGSSTHLDNLPSSPTSPMPGQLFGISLPNICENDNLPKPVLDMLFFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGVEVHLDCESIFVIASVLKDFLRNIPGSIFSSDLYDHWVSVMDQGNDEEKINTVQRLLDQLPRANVVLLRYLFGVLHNIEQHSSSNQMTAFNLAVCVAPSILWPPASSSPELENEFTKKVSLLIQFLIENCLRIFGEEITSLFREVSVRCDTRENASDISCFQLNDSSYDSLENELNEDVDAPCSDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLAHSKDEDVQMKRPLESKPVNILVYTKIPLRDHARAPSAMCTPSYLSTAAANAAKSLRRHRRCSEPSIDYLDSKLSYLREFYQKKLRKSSCDAILSQKDEDYLKQNQPLQEEGKTCFKQSLVTGTDVSKKNATTQNTKKKSLSGSEGNHVKLFPKSKPVAISVASYSPMSSQDHSKNQPFDVNTSGYSPPHTADALKGPRTHRRCSEPNIEDQNRKLTYLRGIYSKKQHKTSCEAGLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNTEKVLPPRLNLCPRTSYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQAQRKREDLSPDFSNASHVSGMPGPSSGQACSRPAYTKKDTMEWHSQMHSVTLHPSTWLRNGVASLKNWSLKKKAKAARPEEEKIASPKGPLEPPPHASGVPEANSLQEEQKDLPLRAAEGLSPVQSAQRCSSSPFQDSERHCSSPFSLVESRLKLCMKSHEEIEPGSQSSSGSLPWERASASSWTLEDATSPDSGPTVVCDIEDRYLTKDI

Target ORF information:

RefSeq Version	NM_001258416.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258416.1

ORF Insert Sequence:

ATGACATTTT GGATTATAAT AAATAAGAAA ATGAAAACAC TAGCAGAAAG GAGGAGGAGC 
GCTCCATCTC TTATCCTGGA TAAAGCCCTA CAAAAACGGC CTACTACCAG GGACAGTCCT 
TCTGCTAGTG TTGACACATG CACATTTCTG TCATCATTAG TGTGCTCCAA TAGGACTCTG 
CTGATTGATG GCCGGGCAGA ACTCAAAAGA GGCCTCCAGA GGCAGGAGCG GCATCTTTTC 
CTATTCAATG ATCTGTTTGT TGTGGCCAAA ATCAAATATA ACAATAACTT TAAGATAAAA 
AATAAAATTA AATTAACTGA TATGTGGACA GCAAGCTGTG TGGATGAAGT GGGAGAAGGC 
AACACCAATG CCATGAAATC CTTTGTTTTG GGCTGGCCCA CAGTGAACTT TGTGGCCACT 
TTCAGTTCTC CAGAACAAAA GGACAAATGG CTCTCTCTCC TTCAGAGATA CATCAATCTA 
GAGAAAGAAA AGGACTACCC GAAGAGCATT CCCCTCAAAA TCTTCGCCAA GGACATTGGG 
AATTGTGCCT ACTCTAAAAC TATAACAGTA ATGAATTCAG ATACAGCGAA TGAAGTTATC 
AACATGTCAT TACCAATGCT AGGGATAACT GGCTCTGAGA GAGATTACCA GTTGTGGGTC 
AATTCTGGCA AAGAAGAGGC TCCATACCCA CTCATTGGGC ATGAATATCC ATATGGAATT 
AAAATGAGCC ATCTTCGAGA CTCTGCACTC CTGACACCGG GATCAAAGGA CTCTACCACC 
CCTTTCAACC TCCAGGAGCC CTTCCTTATG GAACAGCTCC CCCGAGAGAT GCAGTGCCAG 
TTCATCCTGA AGCCCAGCCG CCTGGCTGCA GCCCAGCAAC TGAGTGATTC AGGTCATAAG 
ACATTTAAAA GGAGAAGATC TATCATAAAC TGGGCCTTCT GGCGAGGTTC TAGCACTCAC 
CTGGACAACT TGCCCTCATC GCCAACATCA CCTATGCCAG GACAGCTCTT TGGAATTTCT 
CTGCCAAATA TTTGTGAGAA TGACAATCTG CCCAAACCTG TCTTGGATAT GCTTTTCTTT 
CTTAATCAAA AAGGACCTCT CACCAAAGGT ATCTTCAGGC AATCAGCCAA TGTGAAATCC 
TGCAGAGAAC TAAAAGAGAA ATTGAATTCT GGAGTCGAAG TACACCTAGA CTGTGAATCT 
ATTTTTGTGA TAGCATCTGT CTTAAAGGAT TTTCTGCGAA ATATTCCAGG AAGTATTTTT 
TCATCAGATC TCTATGATCA CTGGGTCTCT GTAATGGATC AAGGAAATGA TGAAGAGAAA 
ATAAATACTG TTCAAAGGCT ATTAGACCAG CTTCCGAGAG CCAATGTTGT TCTCCTAAGG 
TATCTTTTTG GGGTGTTACA CAACATTGAG CAACATTCCT CATCCAATCA GATGACTGCA 
TTTAATTTAG CTGTGTGTGT CGCTCCAAGT ATTCTTTGGC CTCCTGCTTC CTCCAGCCCA 
GAACTAGAAA ACGAATTTAC AAAAAAGGTT TCCCTGCTTA TACAATTTCT GATTGAGAAT 
TGCCTTAGGA TATTTGGAGA AGAAATCACT TCCCTCTTCA GAGAGGTTTC AGTGAGATGT 
GACACTAGAG AGAATGCCTC AGATATTTCT TGCTTTCAAC TGAATGACTC CTCCTATGAC 
AGCTTGGAAA ATGAGCTAAA TGAGGATGTT GATGCACCAT GCAGTGACTT GGTAAAGAAA 
CTTGGCCAGG GGAGCAGAAG CATGGACTCT GTCTTAACCC TCAGTGACTA TGATCTTGAC 
CAGCCCGAGG TGGAAGGCCT TTTAACCCTA AGCGACTTTG ACTTGGCCCA TTCTAAAGAT 
GAAGATGTTC AAATGAAACG GCCTCTTGAA TCCAAGCCGG TGAACATTTT AGTGTACACA 
AAGATCCCAC TGCGGGATCA TGCCAGGGCC CCATCTGCCA TGTGCACACC CAGCTACCTG 
TCCACAGCTG CAGCAAATGC TGCAAAAAGC CTGAGGCGAC ACCGGCGTTG CTCAGAGCCC 
AGCATCGACT ATCTGGATTC AAAGCTTTCC TACCTCAGGG AGTTTTATCA GAAAAAGCTA 
CGCAAGTCCA GCTGTGATGC AATTCTTTCT CAAAAAGATG AAGACTATCT GAAGCAGAAT 
CAACCCCTCC AGGAGGAAGG AAAGACATGT TTTAAACAGA GTTTAGTCAC AGGCACTGAT 
GTCAGCAAGA AAAATGCCAC TACTCAAAAC ACTAAGAAGA AAAGCTTGTC TGGTAGTGAA 
GGAAATCACG TGAAACTTTT CCCTAAGTCT AAGCCAGTGG CCATTTCTGT GGCATCTTAT 
AGTCCTATGT CCTCACAGGA TCATTCCAAG AACCAGCCCT TTGATGTGAA TACATCTGGA 
TACTCCCCAC CACACACAGC AGATGCCCTC AAGGGTCCAA GGACACATCG GCGCTGCTCA 
GAGCCCAACA TAGAAGACCA GAACCGCAAG CTGACCTATC TCAGGGGAAT TTATTCAAAG 
AAACAACATA AAACCAGCTG TGAAGCTGGT CTCTTGCATG GAGAGGAGGA TTATCTCAAA 
CGGCATAAGT CTTTGCAAAT GGAGGGGCAG AAGCTCATTA ATCAGAGTTT AGTCATGGGG 
ATTGAGGTGG GCAAGAGTAG TGCCACAAAC CAAAACACTG AGAAGGTTTT ACCCCCAAGA 
TTAAACCTTT GCCCAAGGAC CAGCTATTCC AGCTTATCCT CCCCAGGCAC TTCCCCATCC 
GGCTCATCAG TAAGCTCCCA AGACAGTGCT TTTTCTCAGA TTTCTGAACA CTCTGTGTTT 
ACACCCACTG AGACTTCCTC TCCAATAGAT TGCACTTTTC AGGCTCAGAG AAAACGGGAA 
GACCTTTCTC CTGACTTTAG CAATGCCAGC CATGTTTCCG GAATGCCCGG TCCCTCATCA 
GGGCAGGCTT GCAGCCGCCC AGCCTATACA AAGAAGGACA CCATGGAGTG GCATTCACAA 
ATGCATTCTG TAACTCTTCA TCCCAGCACA TGGTTGAGAA ATGGTGTGGC CAGTTTGAAA 
AACTGGTCCC TCAAAAAGAA AGCAAAGGCA GCCAGACCAG AGGAAGAGAA AATAGCTTCT 
CCAAAAGGAC CCTTAGAGCC ACCCCCACAT GCTTCTGGTG TTCCAGAAGC CAACTCACTG 
CAAGAGGAAC AAAAAGACTT GCCCTTAAGG GCAGCTGAAG GACTGTCCCC TGTGCAGTCA 
GCCCAAAGGT GTAGTTCTTC TCCCTTCCAG GACTCAGAGA GACACTGTAG CTCTCCATTC 
AGCCTGGTGG AGAGCAGACT TAAGCTGTGC ATGAAGTCAC ATGAGGAAAT AGAGCCTGGT 
AGTCAGAGCT CTTCTGGTTC TCTGCCTTGG GAAAGAGCCT CAGCCAGCTC TTGGACTCTA 
GAGGATGCGA CCAGCCCAGA CTCAGGGCCT ACAGTGGTCT GCGACATTGA GGACAGGTAT 
TTAACCAAAG ACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP20 ( NM_001258418.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001245347.1 Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 5, mRNA.

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CloneID	OHu08022
Clone ID Related Accession (Same CDS sequence)	NM_001258418.1 , NM_001258417.1
Accession Version	NM_001258418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 20 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY496265.1 and AP001883.6. Transcript Variant: This variant (5) differs in the 5' UTR and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 4 and 5 encode the same isoform (4), which has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY496265.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAACAC TAGCAGAAAG GAGGAGGAGC GCTCCATCTC TTATCCTGGA TAAAGCCCTA 
CAAAAACGGC CTACTACCAG GGACAGTCCT TCTGCTAGTG TTGACACATG CACATTTCTG 
TCATCATTAG TGTGCTCCAA TAGGACTCTG CTGATTGATG GCCGGGCAGA ACTCAAAAGA 
GGCCTCCAGA GGCAGGAGCG GCATCTTTTC CTATTCAATG ATCTGTTTGT TGTGGCCAAA 
ATCAAATATA ACAATAACTT TAAGATAAAA AATAAAATTA AATTAACTGA TATGTGGACA 
GCAAGCTGTG TGGATGAAGT GGGAGAAGGC AACACCAATG CCATGAAATC CTTTGTTTTG 
GGCTGGCCCA CAGTGAACTT TGTGGCCACT TTCAGTTCTC CAGAACAAAA GGACAAATGG 
CTCTCTCTCC TTCAGAGATA CATCAATCTA GAGAAAGAAA AGGACTACCC GAAGAGCATT 
CCCCTCAAAA TCTTCGCCAA GGACATTGGG AATTGTGCCT ACTCTAAAAC TATAACAGTA 
ATGAATTCAG ATACAGCGAA TGAAGTTATC AACATGTCAT TACCAATGCT AGGGATAACT 
GGCTCTGAGA GAGATTACCA GTTGTGGGTC AATTCTGGCA AAGAAGAGGC TCCATACCCA 
CTCATTGGGC ATGAATATCC ATATGGAATT AAAATGAGCC ATCTTCGAGA CTCTGCACTC 
CTGACACCGG GATCAAAGGA CTCTACCACC CCTTTCAACC TCCAGGAGCC CTTCCTTATG 
GAACAGCTCC CCCGAGAGAT GCAGTGCCAG TTCATCCTGA AGCCCAGCCG CCTGGCTGCA 
GCCCAGCAAC TGAGTGATTC AGGTCATAAG ACATTTAAAA GGAGAAGATC TATCATAAAC 
TGGGCCTTCT GGCGAGGTTC TAGCACTCAC CTGGACAACT TGCCCTCATC GCCAACATCA 
CCTATGCCAG GACAGCTCTT TGGAATTTCT CTGCCAAATA TTTGTGAGAA TGACAATCTG 
CCCAAACCTG TCTTGGATAT GCTTTTCTTT CTTAATCAAA AAGGACCTCT CACCAAAGGT 
ATCTTCAGGC AATCAGCCAA TGTGAAATCC TGCAGAGAAC TAAAAGAGAA ATTGAATTCT 
GGAGTCGAAG TACACCTAGA CTGTGAATCT ATTTTTGTGA TAGCATCTGT CTTAAAGGAT 
TTTCTGCGAA ATATTCCAGG AAGTATTTTT TCATCAGATC TCTATGATCA CTGGGTCTCT 
GTAATGGATC AAGGAAATGA TGAAGAGAAA ATAAATACTG TTCAAAGGCT ATTAGACCAG 
CTTCCGAGAG CCAATGTTGT TCTCCTAAGG TATCTTTTTG GGGTGTTACA CAACATTGAG 
CAACATTCCT CATCCAATCA GATGACTGCA TTTAATTTAG CTGTGTGTGT CGCTCCAAGT 
ATTCTTTGGC CTCCTGCTTC CTCCAGCCCA GAACTAGAAA ACGAATTTAC AAAAAAGGTT 
TCCCTGCTTA TACAATTTCT GATTGAGAAT TGCCTTAGGA TATTTGGAGA AGAAATCACT 
TCCCTCTTCA GAGAGGTTTC AGTGAGATGT GACACTAGAG AGAATGCCTC AGATATTTCT 
TGCTTTCAAC TGAATGACTC CTCCTATGAC AGCTTGGAAA ATGAGCTAAA TGAGGATGTT 
GATGCACCAT GCAGTGACTT GGTAAAGAAA CTTGGCCAGG GGAGCAGAAG CATGGACTCT 
GTCTTAACCC TCAGTGACTA TGATCTTGAC CAGCCCGAGG TGGAAGGCCT TTTAACCCTA 
AGCGACTTTG ACTTGGCCCA TTCTAAAGAT GAAGATGTTC AAATGAAACG GCCTCTTGAA 
TCCAAGCCGG TGAACATTTT AGTGTACACA AAGATCCCAC TGCGGGATCA TGCCAGGGCC 
CCATCTGCCA TGTGCACACC CAGCTACCTG TCCACAGCTG CAGCAAATGC TGCAAAAAGC 
CTGAGGCGAC ACCGGCGTTG CTCAGAGCCC AGCATCGACT ATCTGGATTC AAAGCTTTCC 
TACCTCAGGG AGTTTTATCA GAAAAAGCTA CGCAAGTCCA GCTGTGATGC AATTCTTTCT 
CAAAAAGATG AAGACTATCT GAAGCAGAAT CAACCCCTCC AGGAGGAAGG AAAGACATGT 
TTTAAACAGA GTTTAGTCAC AGGCACTGAT GTCAGCAAGA AAAATGCCAC TACTCAAAAC 
ACTAAGAAGA AAAGCTTGTC TGGTAGTGAA GGAAATCACG TGAAACTTTT CCCTAAGTCT 
AAGCCAGTGG CCATTTCTGT GGCATCTTAT AGTCCTATGT CCTCACAGGA TCATTCCAAG 
AACCAGCCCT TTGATGTGAA TACATCTGGA TACTCCCCAC CACACACAGC AGATGCCCTC 
AAGGGTCCAA GGACACATCG GCGCTGCTCA GAGCCCAACA TAGAAGACCA GAACCGCAAG 
CTGACCTATC TCAGGGGAAT TTATTCAAAG AAACAACATA AAACCAGCTG TGAAGCTGGT 
CTCTTGCATG GAGAGGAGGA TTATCTCAAA CGGCATAAGT CTTTGCAAAT GGAGGGGCAG 
AAGCTCATTA ATCAGAGTTT AGTCATGGGG ATTGAGGTGG GCAAGAGTAG TGCCACAAAC 
CAAAACACTG AGAAGGTTTT ACCCCCAAGA TTAAACCTTT GCCCAAGGAC CAGCTATTCC 
AGCTTATCCT CCCCAGGCAC TTCCCCATCC GGCTCATCAG TAAGCTCCCA AGACAGTGCT 
TTTTCTCAGA TTTCTGAACA CTCTGTGTTT ACACCCACTG AGACTTCCTC TCCAATAGAT 
TGCACTTTTC AGGCTCAGAG AAAACGGGAA GACCTTTCTC CTGACTTTAG CAATGCCAGC 
CATGTTTCCG GAATGCCCGG TCCCTCATCA GGGCAGGCTT GCAGCCGCCC AGCCTATACA 
AAGAAGGACA CCATGGAGTG GCATTCACAA ATGCATTCTG TAACTCTTCA TCCCAGCACA 
TGGTTGAGAA ATGGTGTGGC CAGTTTGAAA AACTGGTCCC TCAAAAAGAA AGCAAAGGCA 
GCCAGACCAG AGGAAGAGAA AATAGCTTCT CCAAAAGGAC CCTTAGAGCC ACCCCCACAT 
GCTTCTGGTG TTCCAGAAGC CAACTCACTG CAAGAGGAAC AAAAAGACTT GCCCTTAAGG 
GCAGCTGAAG GACTGTCCCC TGTGCAGTCA GCCCAAAGGT GTAGTTCTTC TCCCTTCCAG 
GACTCAGAGA GACACTGTAG CTCTCCATTC AGCCTGGTGG AGAGCAGACT TAAGCTGTGC 
ATGAAGTCAC ATGAGGAAAT AGAGCCTGGT AGTCAGAGCT CTTCTGGTTC TCTGCCTTGG 
GAAAGAGCCT CAGCCAGCTC TTGGACTCTA GAGGATGCGA CCAGCCCAGA CTCAGGGCCT 
ACAGTGGTCT GCGACATTGA GGACAGGTAT TTAACCAAAG ACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245347.1
CDS	215..3682
Translation	MKTLAERRRSAPSLILDKALQKRPTTRDSPSASVDTCTFLSSLVCSNRTLLIDGRAELKRGLQRQERHLFLFNDLFVVAKIKYNNNFKIKNKIKLTDMWTASCVDEVGEGNTNAMKSFVLGWPTVNFVATFSSPEQKDKWLSLLQRYINLEKEKDYPKSIPLKIFAKDIGNCAYSKTITVMNSDTANEVINMSLPMLGITGSERDYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDSALLTPGSKDSTTPFNLQEPFLMEQLPREMQCQFILKPSRLAAAQQLSDSGHKTFKRRRSIINWAFWRGSSTHLDNLPSSPTSPMPGQLFGISLPNICENDNLPKPVLDMLFFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGVEVHLDCESIFVIASVLKDFLRNIPGSIFSSDLYDHWVSVMDQGNDEEKINTVQRLLDQLPRANVVLLRYLFGVLHNIEQHSSSNQMTAFNLAVCVAPSILWPPASSSPELENEFTKKVSLLIQFLIENCLRIFGEEITSLFREVSVRCDTRENASDISCFQLNDSSYDSLENELNEDVDAPCSDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLAHSKDEDVQMKRPLESKPVNILVYTKIPLRDHARAPSAMCTPSYLSTAAANAAKSLRRHRRCSEPSIDYLDSKLSYLREFYQKKLRKSSCDAILSQKDEDYLKQNQPLQEEGKTCFKQSLVTGTDVSKKNATTQNTKKKSLSGSEGNHVKLFPKSKPVAISVASYSPMSSQDHSKNQPFDVNTSGYSPPHTADALKGPRTHRRCSEPNIEDQNRKLTYLRGIYSKKQHKTSCEAGLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNTEKVLPPRLNLCPRTSYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQAQRKREDLSPDFSNASHVSGMPGPSSGQACSRPAYTKKDTMEWHSQMHSVTLHPSTWLRNGVASLKNWSLKKKAKAARPEEEKIASPKGPLEPPPHASGVPEANSLQEEQKDLPLRAAEGLSPVQSAQRCSSSPFQDSERHCSSPFSLVESRLKLCMKSHEEIEPGSQSSSGSLPWERASASSWTLEDATSPDSGPTVVCDIEDRYLTKDI

Target ORF information:

RefSeq Version	NM_001258418.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258418.1

ORF Insert Sequence:

ATGAAAACAC TAGCAGAAAG GAGGAGGAGC GCTCCATCTC TTATCCTGGA TAAAGCCCTA 
CAAAAACGGC CTACTACCAG GGACAGTCCT TCTGCTAGTG TTGACACATG CACATTTCTG 
TCATCATTAG TGTGCTCCAA TAGGACTCTG CTGATTGATG GCCGGGCAGA ACTCAAAAGA 
GGCCTCCAGA GGCAGGAGCG GCATCTTTTC CTATTCAATG ATCTGTTTGT TGTGGCCAAA 
ATCAAATATA ACAATAACTT TAAGATAAAA AATAAAATTA AATTAACTGA TATGTGGACA 
GCAAGCTGTG TGGATGAAGT GGGAGAAGGC AACACCAATG CCATGAAATC CTTTGTTTTG 
GGCTGGCCCA CAGTGAACTT TGTGGCCACT TTCAGTTCTC CAGAACAAAA GGACAAATGG 
CTCTCTCTCC TTCAGAGATA CATCAATCTA GAGAAAGAAA AGGACTACCC GAAGAGCATT 
CCCCTCAAAA TCTTCGCCAA GGACATTGGG AATTGTGCCT ACTCTAAAAC TATAACAGTA 
ATGAATTCAG ATACAGCGAA TGAAGTTATC AACATGTCAT TACCAATGCT AGGGATAACT 
GGCTCTGAGA GAGATTACCA GTTGTGGGTC AATTCTGGCA AAGAAGAGGC TCCATACCCA 
CTCATTGGGC ATGAATATCC ATATGGAATT AAAATGAGCC ATCTTCGAGA CTCTGCACTC 
CTGACACCGG GATCAAAGGA CTCTACCACC CCTTTCAACC TCCAGGAGCC CTTCCTTATG 
GAACAGCTCC CCCGAGAGAT GCAGTGCCAG TTCATCCTGA AGCCCAGCCG CCTGGCTGCA 
GCCCAGCAAC TGAGTGATTC AGGTCATAAG ACATTTAAAA GGAGAAGATC TATCATAAAC 
TGGGCCTTCT GGCGAGGTTC TAGCACTCAC CTGGACAACT TGCCCTCATC GCCAACATCA 
CCTATGCCAG GACAGCTCTT TGGAATTTCT CTGCCAAATA TTTGTGAGAA TGACAATCTG 
CCCAAACCTG TCTTGGATAT GCTTTTCTTT CTTAATCAAA AAGGACCTCT CACCAAAGGT 
ATCTTCAGGC AATCAGCCAA TGTGAAATCC TGCAGAGAAC TAAAAGAGAA ATTGAATTCT 
GGAGTCGAAG TACACCTAGA CTGTGAATCT ATTTTTGTGA TAGCATCTGT CTTAAAGGAT 
TTTCTGCGAA ATATTCCAGG AAGTATTTTT TCATCAGATC TCTATGATCA CTGGGTCTCT 
GTAATGGATC AAGGAAATGA TGAAGAGAAA ATAAATACTG TTCAAAGGCT ATTAGACCAG 
CTTCCGAGAG CCAATGTTGT TCTCCTAAGG TATCTTTTTG GGGTGTTACA CAACATTGAG 
CAACATTCCT CATCCAATCA GATGACTGCA TTTAATTTAG CTGTGTGTGT CGCTCCAAGT 
ATTCTTTGGC CTCCTGCTTC CTCCAGCCCA GAACTAGAAA ACGAATTTAC AAAAAAGGTT 
TCCCTGCTTA TACAATTTCT GATTGAGAAT TGCCTTAGGA TATTTGGAGA AGAAATCACT 
TCCCTCTTCA GAGAGGTTTC AGTGAGATGT GACACTAGAG AGAATGCCTC AGATATTTCT 
TGCTTTCAAC TGAATGACTC CTCCTATGAC AGCTTGGAAA ATGAGCTAAA TGAGGATGTT 
GATGCACCAT GCAGTGACTT GGTAAAGAAA CTTGGCCAGG GGAGCAGAAG CATGGACTCT 
GTCTTAACCC TCAGTGACTA TGATCTTGAC CAGCCCGAGG TGGAAGGCCT TTTAACCCTA 
AGCGACTTTG ACTTGGCCCA TTCTAAAGAT GAAGATGTTC AAATGAAACG GCCTCTTGAA 
TCCAAGCCGG TGAACATTTT AGTGTACACA AAGATCCCAC TGCGGGATCA TGCCAGGGCC 
CCATCTGCCA TGTGCACACC CAGCTACCTG TCCACAGCTG CAGCAAATGC TGCAAAAAGC 
CTGAGGCGAC ACCGGCGTTG CTCAGAGCCC AGCATCGACT ATCTGGATTC AAAGCTTTCC 
TACCTCAGGG AGTTTTATCA GAAAAAGCTA CGCAAGTCCA GCTGTGATGC AATTCTTTCT 
CAAAAAGATG AAGACTATCT GAAGCAGAAT CAACCCCTCC AGGAGGAAGG AAAGACATGT 
TTTAAACAGA GTTTAGTCAC AGGCACTGAT GTCAGCAAGA AAAATGCCAC TACTCAAAAC 
ACTAAGAAGA AAAGCTTGTC TGGTAGTGAA GGAAATCACG TGAAACTTTT CCCTAAGTCT 
AAGCCAGTGG CCATTTCTGT GGCATCTTAT AGTCCTATGT CCTCACAGGA TCATTCCAAG 
AACCAGCCCT TTGATGTGAA TACATCTGGA TACTCCCCAC CACACACAGC AGATGCCCTC 
AAGGGTCCAA GGACACATCG GCGCTGCTCA GAGCCCAACA TAGAAGACCA GAACCGCAAG 
CTGACCTATC TCAGGGGAAT TTATTCAAAG AAACAACATA AAACCAGCTG TGAAGCTGGT 
CTCTTGCATG GAGAGGAGGA TTATCTCAAA CGGCATAAGT CTTTGCAAAT GGAGGGGCAG 
AAGCTCATTA ATCAGAGTTT AGTCATGGGG ATTGAGGTGG GCAAGAGTAG TGCCACAAAC 
CAAAACACTG AGAAGGTTTT ACCCCCAAGA TTAAACCTTT GCCCAAGGAC CAGCTATTCC 
AGCTTATCCT CCCCAGGCAC TTCCCCATCC GGCTCATCAG TAAGCTCCCA AGACAGTGCT 
TTTTCTCAGA TTTCTGAACA CTCTGTGTTT ACACCCACTG AGACTTCCTC TCCAATAGAT 
TGCACTTTTC AGGCTCAGAG AAAACGGGAA GACCTTTCTC CTGACTTTAG CAATGCCAGC 
CATGTTTCCG GAATGCCCGG TCCCTCATCA GGGCAGGCTT GCAGCCGCCC AGCCTATACA 
AAGAAGGACA CCATGGAGTG GCATTCACAA ATGCATTCTG TAACTCTTCA TCCCAGCACA 
TGGTTGAGAA ATGGTGTGGC CAGTTTGAAA AACTGGTCCC TCAAAAAGAA AGCAAAGGCA 
GCCAGACCAG AGGAAGAGAA AATAGCTTCT CCAAAAGGAC CCTTAGAGCC ACCCCCACAT 
GCTTCTGGTG TTCCAGAAGC CAACTCACTG CAAGAGGAAC AAAAAGACTT GCCCTTAAGG 
GCAGCTGAAG GACTGTCCCC TGTGCAGTCA GCCCAAAGGT GTAGTTCTTC TCCCTTCCAG 
GACTCAGAGA GACACTGTAG CTCTCCATTC AGCCTGGTGG AGAGCAGACT TAAGCTGTGC 
ATGAAGTCAC ATGAGGAAAT AGAGCCTGGT AGTCAGAGCT CTTCTGGTTC TCTGCCTTGG 
GAAAGAGCCT CAGCCAGCTC TTGGACTCTA GAGGATGCGA CCAGCCCAGA CTCAGGGCCT 
ACAGTGGTCT GCGACATTGA GGACAGGTAT TTAACCAAAG ACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP20 ( NM_001258417.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001245346.1 Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu08022
Clone ID Related Accession (Same CDS sequence)	NM_001258418.1 , NM_001258417.1
Accession Version	NM_001258417.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 20 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY496264.1 and AP001883.6. Transcript Variant: This variant (4) differs in the 5' UTR and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 4 and 5 encode the same isoform (4), which has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY496264.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAACAC TAGCAGAAAG GAGGAGGAGC GCTCCATCTC TTATCCTGGA TAAAGCCCTA 
CAAAAACGGC CTACTACCAG GGACAGTCCT TCTGCTAGTG TTGACACATG CACATTTCTG 
TCATCATTAG TGTGCTCCAA TAGGACTCTG CTGATTGATG GCCGGGCAGA ACTCAAAAGA 
GGCCTCCAGA GGCAGGAGCG GCATCTTTTC CTATTCAATG ATCTGTTTGT TGTGGCCAAA 
ATCAAATATA ACAATAACTT TAAGATAAAA AATAAAATTA AATTAACTGA TATGTGGACA 
GCAAGCTGTG TGGATGAAGT GGGAGAAGGC AACACCAATG CCATGAAATC CTTTGTTTTG 
GGCTGGCCCA CAGTGAACTT TGTGGCCACT TTCAGTTCTC CAGAACAAAA GGACAAATGG 
CTCTCTCTCC TTCAGAGATA CATCAATCTA GAGAAAGAAA AGGACTACCC GAAGAGCATT 
CCCCTCAAAA TCTTCGCCAA GGACATTGGG AATTGTGCCT ACTCTAAAAC TATAACAGTA 
ATGAATTCAG ATACAGCGAA TGAAGTTATC AACATGTCAT TACCAATGCT AGGGATAACT 
GGCTCTGAGA GAGATTACCA GTTGTGGGTC AATTCTGGCA AAGAAGAGGC TCCATACCCA 
CTCATTGGGC ATGAATATCC ATATGGAATT AAAATGAGCC ATCTTCGAGA CTCTGCACTC 
CTGACACCGG GATCAAAGGA CTCTACCACC CCTTTCAACC TCCAGGAGCC CTTCCTTATG 
GAACAGCTCC CCCGAGAGAT GCAGTGCCAG TTCATCCTGA AGCCCAGCCG CCTGGCTGCA 
GCCCAGCAAC TGAGTGATTC AGGTCATAAG ACATTTAAAA GGAGAAGATC TATCATAAAC 
TGGGCCTTCT GGCGAGGTTC TAGCACTCAC CTGGACAACT TGCCCTCATC GCCAACATCA 
CCTATGCCAG GACAGCTCTT TGGAATTTCT CTGCCAAATA TTTGTGAGAA TGACAATCTG 
CCCAAACCTG TCTTGGATAT GCTTTTCTTT CTTAATCAAA AAGGACCTCT CACCAAAGGT 
ATCTTCAGGC AATCAGCCAA TGTGAAATCC TGCAGAGAAC TAAAAGAGAA ATTGAATTCT 
GGAGTCGAAG TACACCTAGA CTGTGAATCT ATTTTTGTGA TAGCATCTGT CTTAAAGGAT 
TTTCTGCGAA ATATTCCAGG AAGTATTTTT TCATCAGATC TCTATGATCA CTGGGTCTCT 
GTAATGGATC AAGGAAATGA TGAAGAGAAA ATAAATACTG TTCAAAGGCT ATTAGACCAG 
CTTCCGAGAG CCAATGTTGT TCTCCTAAGG TATCTTTTTG GGGTGTTACA CAACATTGAG 
CAACATTCCT CATCCAATCA GATGACTGCA TTTAATTTAG CTGTGTGTGT CGCTCCAAGT 
ATTCTTTGGC CTCCTGCTTC CTCCAGCCCA GAACTAGAAA ACGAATTTAC AAAAAAGGTT 
TCCCTGCTTA TACAATTTCT GATTGAGAAT TGCCTTAGGA TATTTGGAGA AGAAATCACT 
TCCCTCTTCA GAGAGGTTTC AGTGAGATGT GACACTAGAG AGAATGCCTC AGATATTTCT 
TGCTTTCAAC TGAATGACTC CTCCTATGAC AGCTTGGAAA ATGAGCTAAA TGAGGATGTT 
GATGCACCAT GCAGTGACTT GGTAAAGAAA CTTGGCCAGG GGAGCAGAAG CATGGACTCT 
GTCTTAACCC TCAGTGACTA TGATCTTGAC CAGCCCGAGG TGGAAGGCCT TTTAACCCTA 
AGCGACTTTG ACTTGGCCCA TTCTAAAGAT GAAGATGTTC AAATGAAACG GCCTCTTGAA 
TCCAAGCCGG TGAACATTTT AGTGTACACA AAGATCCCAC TGCGGGATCA TGCCAGGGCC 
CCATCTGCCA TGTGCACACC CAGCTACCTG TCCACAGCTG CAGCAAATGC TGCAAAAAGC 
CTGAGGCGAC ACCGGCGTTG CTCAGAGCCC AGCATCGACT ATCTGGATTC AAAGCTTTCC 
TACCTCAGGG AGTTTTATCA GAAAAAGCTA CGCAAGTCCA GCTGTGATGC AATTCTTTCT 
CAAAAAGATG AAGACTATCT GAAGCAGAAT CAACCCCTCC AGGAGGAAGG AAAGACATGT 
TTTAAACAGA GTTTAGTCAC AGGCACTGAT GTCAGCAAGA AAAATGCCAC TACTCAAAAC 
ACTAAGAAGA AAAGCTTGTC TGGTAGTGAA GGAAATCACG TGAAACTTTT CCCTAAGTCT 
AAGCCAGTGG CCATTTCTGT GGCATCTTAT AGTCCTATGT CCTCACAGGA TCATTCCAAG 
AACCAGCCCT TTGATGTGAA TACATCTGGA TACTCCCCAC CACACACAGC AGATGCCCTC 
AAGGGTCCAA GGACACATCG GCGCTGCTCA GAGCCCAACA TAGAAGACCA GAACCGCAAG 
CTGACCTATC TCAGGGGAAT TTATTCAAAG AAACAACATA AAACCAGCTG TGAAGCTGGT 
CTCTTGCATG GAGAGGAGGA TTATCTCAAA CGGCATAAGT CTTTGCAAAT GGAGGGGCAG 
AAGCTCATTA ATCAGAGTTT AGTCATGGGG ATTGAGGTGG GCAAGAGTAG TGCCACAAAC 
CAAAACACTG AGAAGGTTTT ACCCCCAAGA TTAAACCTTT GCCCAAGGAC CAGCTATTCC 
AGCTTATCCT CCCCAGGCAC TTCCCCATCC GGCTCATCAG TAAGCTCCCA AGACAGTGCT 
TTTTCTCAGA TTTCTGAACA CTCTGTGTTT ACACCCACTG AGACTTCCTC TCCAATAGAT 
TGCACTTTTC AGGCTCAGAG AAAACGGGAA GACCTTTCTC CTGACTTTAG CAATGCCAGC 
CATGTTTCCG GAATGCCCGG TCCCTCATCA GGGCAGGCTT GCAGCCGCCC AGCCTATACA 
AAGAAGGACA CCATGGAGTG GCATTCACAA ATGCATTCTG TAACTCTTCA TCCCAGCACA 
TGGTTGAGAA ATGGTGTGGC CAGTTTGAAA AACTGGTCCC TCAAAAAGAA AGCAAAGGCA 
GCCAGACCAG AGGAAGAGAA AATAGCTTCT CCAAAAGGAC CCTTAGAGCC ACCCCCACAT 
GCTTCTGGTG TTCCAGAAGC CAACTCACTG CAAGAGGAAC AAAAAGACTT GCCCTTAAGG 
GCAGCTGAAG GACTGTCCCC TGTGCAGTCA GCCCAAAGGT GTAGTTCTTC TCCCTTCCAG 
GACTCAGAGA GACACTGTAG CTCTCCATTC AGCCTGGTGG AGAGCAGACT TAAGCTGTGC 
ATGAAGTCAC ATGAGGAAAT AGAGCCTGGT AGTCAGAGCT CTTCTGGTTC TCTGCCTTGG 
GAAAGAGCCT CAGCCAGCTC TTGGACTCTA GAGGATGCGA CCAGCCCAGA CTCAGGGCCT 
ACAGTGGTCT GCGACATTGA GGACAGGTAT TTAACCAAAG ACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245346.1
CDS	97..3564
Translation	MKTLAERRRSAPSLILDKALQKRPTTRDSPSASVDTCTFLSSLVCSNRTLLIDGRAELKRGLQRQERHLFLFNDLFVVAKIKYNNNFKIKNKIKLTDMWTASCVDEVGEGNTNAMKSFVLGWPTVNFVATFSSPEQKDKWLSLLQRYINLEKEKDYPKSIPLKIFAKDIGNCAYSKTITVMNSDTANEVINMSLPMLGITGSERDYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDSALLTPGSKDSTTPFNLQEPFLMEQLPREMQCQFILKPSRLAAAQQLSDSGHKTFKRRRSIINWAFWRGSSTHLDNLPSSPTSPMPGQLFGISLPNICENDNLPKPVLDMLFFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGVEVHLDCESIFVIASVLKDFLRNIPGSIFSSDLYDHWVSVMDQGNDEEKINTVQRLLDQLPRANVVLLRYLFGVLHNIEQHSSSNQMTAFNLAVCVAPSILWPPASSSPELENEFTKKVSLLIQFLIENCLRIFGEEITSLFREVSVRCDTRENASDISCFQLNDSSYDSLENELNEDVDAPCSDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLAHSKDEDVQMKRPLESKPVNILVYTKIPLRDHARAPSAMCTPSYLSTAAANAAKSLRRHRRCSEPSIDYLDSKLSYLREFYQKKLRKSSCDAILSQKDEDYLKQNQPLQEEGKTCFKQSLVTGTDVSKKNATTQNTKKKSLSGSEGNHVKLFPKSKPVAISVASYSPMSSQDHSKNQPFDVNTSGYSPPHTADALKGPRTHRRCSEPNIEDQNRKLTYLRGIYSKKQHKTSCEAGLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNTEKVLPPRLNLCPRTSYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQAQRKREDLSPDFSNASHVSGMPGPSSGQACSRPAYTKKDTMEWHSQMHSVTLHPSTWLRNGVASLKNWSLKKKAKAARPEEEKIASPKGPLEPPPHASGVPEANSLQEEQKDLPLRAAEGLSPVQSAQRCSSSPFQDSERHCSSPFSLVESRLKLCMKSHEEIEPGSQSSSGSLPWERASASSWTLEDATSPDSGPTVVCDIEDRYLTKDI

Target ORF information:

RefSeq Version	NM_001258417.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258417.1

ORF Insert Sequence:

ATGAAAACAC TAGCAGAAAG GAGGAGGAGC GCTCCATCTC TTATCCTGGA TAAAGCCCTA 
CAAAAACGGC CTACTACCAG GGACAGTCCT TCTGCTAGTG TTGACACATG CACATTTCTG 
TCATCATTAG TGTGCTCCAA TAGGACTCTG CTGATTGATG GCCGGGCAGA ACTCAAAAGA 
GGCCTCCAGA GGCAGGAGCG GCATCTTTTC CTATTCAATG ATCTGTTTGT TGTGGCCAAA 
ATCAAATATA ACAATAACTT TAAGATAAAA AATAAAATTA AATTAACTGA TATGTGGACA 
GCAAGCTGTG TGGATGAAGT GGGAGAAGGC AACACCAATG CCATGAAATC CTTTGTTTTG 
GGCTGGCCCA CAGTGAACTT TGTGGCCACT TTCAGTTCTC CAGAACAAAA GGACAAATGG 
CTCTCTCTCC TTCAGAGATA CATCAATCTA GAGAAAGAAA AGGACTACCC GAAGAGCATT 
CCCCTCAAAA TCTTCGCCAA GGACATTGGG AATTGTGCCT ACTCTAAAAC TATAACAGTA 
ATGAATTCAG ATACAGCGAA TGAAGTTATC AACATGTCAT TACCAATGCT AGGGATAACT 
GGCTCTGAGA GAGATTACCA GTTGTGGGTC AATTCTGGCA AAGAAGAGGC TCCATACCCA 
CTCATTGGGC ATGAATATCC ATATGGAATT AAAATGAGCC ATCTTCGAGA CTCTGCACTC 
CTGACACCGG GATCAAAGGA CTCTACCACC CCTTTCAACC TCCAGGAGCC CTTCCTTATG 
GAACAGCTCC CCCGAGAGAT GCAGTGCCAG TTCATCCTGA AGCCCAGCCG CCTGGCTGCA 
GCCCAGCAAC TGAGTGATTC AGGTCATAAG ACATTTAAAA GGAGAAGATC TATCATAAAC 
TGGGCCTTCT GGCGAGGTTC TAGCACTCAC CTGGACAACT TGCCCTCATC GCCAACATCA 
CCTATGCCAG GACAGCTCTT TGGAATTTCT CTGCCAAATA TTTGTGAGAA TGACAATCTG 
CCCAAACCTG TCTTGGATAT GCTTTTCTTT CTTAATCAAA AAGGACCTCT CACCAAAGGT 
ATCTTCAGGC AATCAGCCAA TGTGAAATCC TGCAGAGAAC TAAAAGAGAA ATTGAATTCT 
GGAGTCGAAG TACACCTAGA CTGTGAATCT ATTTTTGTGA TAGCATCTGT CTTAAAGGAT 
TTTCTGCGAA ATATTCCAGG AAGTATTTTT TCATCAGATC TCTATGATCA CTGGGTCTCT 
GTAATGGATC AAGGAAATGA TGAAGAGAAA ATAAATACTG TTCAAAGGCT ATTAGACCAG 
CTTCCGAGAG CCAATGTTGT TCTCCTAAGG TATCTTTTTG GGGTGTTACA CAACATTGAG 
CAACATTCCT CATCCAATCA GATGACTGCA TTTAATTTAG CTGTGTGTGT CGCTCCAAGT 
ATTCTTTGGC CTCCTGCTTC CTCCAGCCCA GAACTAGAAA ACGAATTTAC AAAAAAGGTT 
TCCCTGCTTA TACAATTTCT GATTGAGAAT TGCCTTAGGA TATTTGGAGA AGAAATCACT 
TCCCTCTTCA GAGAGGTTTC AGTGAGATGT GACACTAGAG AGAATGCCTC AGATATTTCT 
TGCTTTCAAC TGAATGACTC CTCCTATGAC AGCTTGGAAA ATGAGCTAAA TGAGGATGTT 
GATGCACCAT GCAGTGACTT GGTAAAGAAA CTTGGCCAGG GGAGCAGAAG CATGGACTCT 
GTCTTAACCC TCAGTGACTA TGATCTTGAC CAGCCCGAGG TGGAAGGCCT TTTAACCCTA 
AGCGACTTTG ACTTGGCCCA TTCTAAAGAT GAAGATGTTC AAATGAAACG GCCTCTTGAA 
TCCAAGCCGG TGAACATTTT AGTGTACACA AAGATCCCAC TGCGGGATCA TGCCAGGGCC 
CCATCTGCCA TGTGCACACC CAGCTACCTG TCCACAGCTG CAGCAAATGC TGCAAAAAGC 
CTGAGGCGAC ACCGGCGTTG CTCAGAGCCC AGCATCGACT ATCTGGATTC AAAGCTTTCC 
TACCTCAGGG AGTTTTATCA GAAAAAGCTA CGCAAGTCCA GCTGTGATGC AATTCTTTCT 
CAAAAAGATG AAGACTATCT GAAGCAGAAT CAACCCCTCC AGGAGGAAGG AAAGACATGT 
TTTAAACAGA GTTTAGTCAC AGGCACTGAT GTCAGCAAGA AAAATGCCAC TACTCAAAAC 
ACTAAGAAGA AAAGCTTGTC TGGTAGTGAA GGAAATCACG TGAAACTTTT CCCTAAGTCT 
AAGCCAGTGG CCATTTCTGT GGCATCTTAT AGTCCTATGT CCTCACAGGA TCATTCCAAG 
AACCAGCCCT TTGATGTGAA TACATCTGGA TACTCCCCAC CACACACAGC AGATGCCCTC 
AAGGGTCCAA GGACACATCG GCGCTGCTCA GAGCCCAACA TAGAAGACCA GAACCGCAAG 
CTGACCTATC TCAGGGGAAT TTATTCAAAG AAACAACATA AAACCAGCTG TGAAGCTGGT 
CTCTTGCATG GAGAGGAGGA TTATCTCAAA CGGCATAAGT CTTTGCAAAT GGAGGGGCAG 
AAGCTCATTA ATCAGAGTTT AGTCATGGGG ATTGAGGTGG GCAAGAGTAG TGCCACAAAC 
CAAAACACTG AGAAGGTTTT ACCCCCAAGA TTAAACCTTT GCCCAAGGAC CAGCTATTCC 
AGCTTATCCT CCCCAGGCAC TTCCCCATCC GGCTCATCAG TAAGCTCCCA AGACAGTGCT 
TTTTCTCAGA TTTCTGAACA CTCTGTGTTT ACACCCACTG AGACTTCCTC TCCAATAGAT 
TGCACTTTTC AGGCTCAGAG AAAACGGGAA GACCTTTCTC CTGACTTTAG CAATGCCAGC 
CATGTTTCCG GAATGCCCGG TCCCTCATCA GGGCAGGCTT GCAGCCGCCC AGCCTATACA 
AAGAAGGACA CCATGGAGTG GCATTCACAA ATGCATTCTG TAACTCTTCA TCCCAGCACA 
TGGTTGAGAA ATGGTGTGGC CAGTTTGAAA AACTGGTCCC TCAAAAAGAA AGCAAAGGCA 
GCCAGACCAG AGGAAGAGAA AATAGCTTCT CCAAAAGGAC CCTTAGAGCC ACCCCCACAT 
GCTTCTGGTG TTCCAGAAGC CAACTCACTG CAAGAGGAAC AAAAAGACTT GCCCTTAAGG 
GCAGCTGAAG GACTGTCCCC TGTGCAGTCA GCCCAAAGGT GTAGTTCTTC TCCCTTCCAG 
GACTCAGAGA GACACTGTAG CTCTCCATTC AGCCTGGTGG AGAGCAGACT TAAGCTGTGC 
ATGAAGTCAC ATGAGGAAAT AGAGCCTGGT AGTCAGAGCT CTTCTGGTTC TCTGCCTTGG 
GAAAGAGCCT CAGCCAGCTC TTGGACTCTA GAGGATGCGA CCAGCCCAGA CTCAGGGCCT 
ACAGTGGTCT GCGACATTGA GGACAGGTAT TTAACCAAAG ACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP20 ( NM_020809.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065860.2 Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 1, mRNA.

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CloneID	OHu08682
Clone ID Related Accession (Same CDS sequence)	XM_006718893.3 , NM_020809.3
Accession Version	NM_020809.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 20 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY496263.1 and AP001883.6. This sequence is a reference standard in the RefSeqGene project. On May 2, 2012 this sequence version replaced NM_020809.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY496263.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCGA TGTCCCCCCA GCAGGAGACT CTAGGGGGAC AGCCGGGGCG CTCCTCTTCC 
CTGACAGGAG TGTCTCGGCT CGCGGGAGGC AGCTGCACCA AGAAGAAAAT GAAAACACTA 
GCAGAAAGGA GGAGGAGCGC TCCATCTCTT ATCCTGGATA AAGCCCTACA AAAACGGCCT 
ACTACCAGGG ACAGTCCTTC TGCTAGTGTT GACACATGCA CATTTCTGTC ATCATTAGTG 
TGCTCCAATA GGACTCTGCT GATTGATGGC CGGGCAGAAC TCAAAAGAGG CCTCCAGAGG 
CAGGAGCGGC ATCTTTTCCT ATTCAATGAT CTGTTTGTTG TGGCCAAAAT CAAATATAAC 
AATAACTTTA AGATAAAAAA TAAAATTAAA TTAACTGATA TGTGGACAGC AAGCTGTGTG 
GATGAAGTGG GAGAAGGCAA CACCAATGCC ATGAAATCCT TTGTTTTGGG CTGGCCCACA 
GTGAACTTTG TGGCCACTTT CAGTTCTCCA GAACAAAAGG ACAAATGGCT CTCTCTCCTT 
CAGAGATACA TCAATCTAGA GAAAGAAAAG GACTACCCGA AGAGCATTCC CCTCAAAATC 
TTCGCCAAGG ACATTGGGAA TTGTGCCTAC TCTAAAACTA TAACAGTAAT GAATTCAGAT 
ACAGCGAATG AAGTTATCAA CATGTCATTA CCAATGCTAG GGATAACTGG CTCTGAGAGA 
GATTACCAGT TGTGGGTCAA TTCTGGCAAA GAAGAGGCTC CATACCCACT CATTGGGCAT 
GAATATCCAT ATGGAATTAA AATGAGCCAT CTTCGAGACT CTGCACTCCT GACACCGGGA 
TCAAAGGACT CTACCACCCC TTTCAACCTC CAGGAGCCCT TCCTTATGGA ACAGCTCCCC 
CGAGAGATGC AGTGCCAGTT CATCCTGAAG CCCAGCCGCC TGGCTGCAGC CCAGCAACTG 
AGTGATTCAG GTCATAAGAC ATTTAAAAGG AGAAGATCTA TCATAAACTG GGCCTTCTGG 
CGAGGTTCTA GCACTCACCT GGACAACTTG CCCTCATCGC CAACATCACC TATGCCAGGA 
CAGCTCTTTG GAATTTCTCT GCCAAATATT TGTGAGAATG ACAATCTGCC CAAACCTGTC 
TTGGATATGC TTTTCTTTCT TAATCAAAAA GGACCTCTCA CCAAAGGTAT CTTCAGGCAA 
TCAGCCAATG TGAAATCCTG CAGAGAACTA AAAGAGAAAT TGAATTCTGG AGTCGAAGTA 
CACCTAGACT GTGAATCTAT TTTTGTGATA GCATCTGTCT TAAAGGATTT TCTGCGAAAT 
ATTCCAGGAA GTATTTTTTC ATCAGATCTC TATGATCACT GGGTCTCTGT AATGGATCAA 
GGAAATGATG AAGAGAAAAT AAATACTGTT CAAAGGCTAT TAGACCAGCT TCCGAGAGCC 
AATGTTGTTC TCCTAAGGTA TCTTTTTGGG GTGTTACACA ACATTGAGCA ACATTCCTCA 
TCCAATCAGA TGACTGCATT TAATTTAGCT GTGTGTGTCG CTCCAAGTAT TCTTTGGCCT 
CCTGCTTCCT CCAGCCCAGA ACTAGAAAAC GAATTTACAA AAAAGGTTTC CCTGCTTATA 
CAATTTCTGA TTGAGAATTG CCTTAGGATA TTTGGAGAAG AAATCACTTC CCTCTTCAGA 
GAGGTTTCAG TGAGATGTGA CACTAGAGAG AATGCCTCAG ATATTTCTTG CTTTCAACTG 
AATGACTCCT CCTATGACAG CTTGGAAAAT GAGCTAAATG AGGATGTTGA TGCACCATGC 
AGTGACTTGG TAAAGAAACT TGGCCAGGGG AGCAGAAGCA TGGACTCTGT CTTAACCCTC 
AGTGACTATG ATCTTGACCA GCCCGAGGTG GAAGGCCTTT TAACCCTAAG CGACTTTGAC 
TTGGCCCATT CTAAAGATGA AGATGTTCAA ATGAAACGGC CTCTTGAATC CAAGCCGGTG 
AACATTTTAG TGTACACAAA GATCCCACTG CGGGATCATG CCAGGGCCCC ATCTGCCATG 
TGCACACCCA GCTACCTGTC CACAGCTGCA GCAAATGCTG CAAAAAGCCT GAGGCGACAC 
CGGCGTTGCT CAGAGCCCAG CATCGACTAT CTGGATTCAA AGCTTTCCTA CCTCAGGGAG 
TTTTATCAGA AAAAGCTACG CAAGTCCAGC TGTGATGCAA TTCTTTCTCA AAAAGATGAA 
GACTATCTGA AGCAGAATCA ACCCCTCCAG GAGGAAGGAA AGACATGTTT TAAACAGAGT 
TTAGTCACAG GCACTGATGT CAGCAAGAAA AATGCCACTA CTCAAAACAC TAAGAAGAAA 
AGCTTGTCTG GTAGTGAAGG AAATCACGTG AAACTTTTCC CTAAGTCTAA GCCAGTGGCC 
ATTTCTGTGG CATCTTATAG TCCTATGTCC TCACAGGATC ATTCCAAGAA CCAGCCCTTT 
GATGTGAATA CATCTGGATA CTCCCCACCA CACACAGCAG ATGCCCTCAA GGGTCCAAGG 
ACACATCGGC GCTGCTCAGA GCCCAACATA GAAGACCAGA ACCGCAAGCT GACCTATCTC 
AGGGGAATTT ATTCAAAGAA ACAACATAAA ACCAGCTGTG AAGCTGGTCT CTTGCATGGA 
GAGGAGGATT ATCTCAAACG GCATAAGTCT TTGCAAATGG AGGGGCAGAA GCTCATTAAT 
CAGAGTTTAG TCATGGGGAT TGAGGTGGGC AAGAGTAGTG CCACAAACCA AAACACTGAG 
AAGGTTTTAC CCCCAAGATT AAACCTTTGC CCAAGGACCA GCTATTCCAG CTTATCCTCC 
CCAGGCACTT CCCCATCCGG CTCATCAGTA AGCTCCCAAG ACAGTGCTTT TTCTCAGATT 
TCTGAACACT CTGTGTTTAC ACCCACTGAG ACTTCCTCTC CAATAGATTG CACTTTTCAG 
GCTCAGAGAA AACGGGAAGA CCTTTCTCCT GACTTTAGCA ATGCCAGCCA TGTTTCCGGA 
ATGCCCGGTC CCTCATCAGG GCAGGCTTGC AGCCGCCCAG CCTATACAAA GAAGGACACC 
ATGGAGTGGC ATTCACAAAT GCATTCTGTA ACTCTTCATC CCAGCACATG GTTGAGAAAT 
GGTGTGGCCA GTTTGAAAAA CTGGTCCCTC AAAAAGAAAG CAAAGGCAGC CAGACCAGAG 
GAAGAGAAAA TAGCTTCTCC AAAAGGACCC TTAGAGCCAC CCCCACATGC TTCTGGTGTT 
CCAGAAGCCA ACTCACTGCA AGAGGAACAA AAAGACTTGC CCTTAAGGGC AGCTGAAGGA 
CTGTCCCCTG TGCAGTCAGC CCAAAGGTGT AGTTCTTCTC CCTTCCAGGA CTCAGAGAGA 
CACTGTAGCT CTCCATTCAG CCTGGTGGAG AGCAGACTTA AGCTGTGCAT GAAGTCACAT 
GAGGAAATAG AGCCTGGTAG TCAGAGCTCT TCTGGTTCTC TGCCTTGGGA AAGAGCCTCA 
GCCAGCTCTT GGACTCTAGA GGATGCGACC AGCCCAGACT CAGGGCCTAC AGTGGTCTGC 
GACATTGAGG ACAGGTATTT AACCAAAGAC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065860.2
CDS	286..3861
Translation	MEAMSPQQETLGGQPGRSSSLTGVSRLAGGSCTKKKMKTLAERRRSAPSLILDKALQKRPTTRDSPSASVDTCTFLSSLVCSNRTLLIDGRAELKRGLQRQERHLFLFNDLFVVAKIKYNNNFKIKNKIKLTDMWTASCVDEVGEGNTNAMKSFVLGWPTVNFVATFSSPEQKDKWLSLLQRYINLEKEKDYPKSIPLKIFAKDIGNCAYSKTITVMNSDTANEVINMSLPMLGITGSERDYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDSALLTPGSKDSTTPFNLQEPFLMEQLPREMQCQFILKPSRLAAAQQLSDSGHKTFKRRRSIINWAFWRGSSTHLDNLPSSPTSPMPGQLFGISLPNICENDNLPKPVLDMLFFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGVEVHLDCESIFVIASVLKDFLRNIPGSIFSSDLYDHWVSVMDQGNDEEKINTVQRLLDQLPRANVVLLRYLFGVLHNIEQHSSSNQMTAFNLAVCVAPSILWPPASSSPELENEFTKKVSLLIQFLIENCLRIFGEEITSLFREVSVRCDTRENASDISCFQLNDSSYDSLENELNEDVDAPCSDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLAHSKDEDVQMKRPLESKPVNILVYTKIPLRDHARAPSAMCTPSYLSTAAANAAKSLRRHRRCSEPSIDYLDSKLSYLREFYQKKLRKSSCDAILSQKDEDYLKQNQPLQEEGKTCFKQSLVTGTDVSKKNATTQNTKKKSLSGSEGNHVKLFPKSKPVAISVASYSPMSSQDHSKNQPFDVNTSGYSPPHTADALKGPRTHRRCSEPNIEDQNRKLTYLRGIYSKKQHKTSCEAGLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNTEKVLPPRLNLCPRTSYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQAQRKREDLSPDFSNASHVSGMPGPSSGQACSRPAYTKKDTMEWHSQMHSVTLHPSTWLRNGVASLKNWSLKKKAKAARPEEEKIASPKGPLEPPPHASGVPEANSLQEEQKDLPLRAAEGLSPVQSAQRCSSSPFQDSERHCSSPFSLVESRLKLCMKSHEEIEPGSQSSSGSLPWERASASSWTLEDATSPDSGPTVVCDIEDRYLTKDI

Target ORF information:

RefSeq Version	NM_020809.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020809.3

ORF Insert Sequence:

ATGGAAGCGA TGTCCCCCCA GCAGGAGACT CTAGGGGGAC AGCCGGGGCG CTCCTCTTCC 
CTGACAGGAG TGTCTCGGCT CGCGGGAGGC AGCTGCACCA AGAAGAAAAT GAAAACACTA 
GCAGAAAGGA GGAGGAGCGC TCCATCTCTT ATCCTGGATA AAGCCCTACA AAAACGGCCT 
ACTACCAGGG ACAGTCCTTC TGCTAGTGTT GACACATGCA CATTTCTGTC ATCATTAGTG 
TGCTCCAATA GGACTCTGCT GATTGATGGC CGGGCAGAAC TCAAAAGAGG CCTCCAGAGG 
CAGGAGCGGC ATCTTTTCCT ATTCAATGAT CTGTTTGTTG TGGCCAAAAT CAAATATAAC 
AATAACTTTA AGATAAAAAA TAAAATTAAA TTAACTGATA TGTGGACAGC AAGCTGTGTG 
GATGAAGTGG GAGAAGGCAA CACCAATGCC ATGAAATCCT TTGTTTTGGG CTGGCCCACA 
GTGAACTTTG TGGCCACTTT CAGTTCTCCA GAACAAAAGG ACAAATGGCT CTCTCTCCTT 
CAGAGATACA TCAATCTAGA GAAAGAAAAG GACTACCCGA AGAGCATTCC CCTCAAAATC 
TTCGCCAAGG ACATTGGGAA TTGTGCCTAC TCTAAAACTA TAACAGTAAT GAATTCAGAT 
ACAGCGAATG AAGTTATCAA CATGTCATTA CCAATGCTAG GGATAACTGG CTCTGAGAGA 
GATTACCAGT TGTGGGTCAA TTCTGGCAAA GAAGAGGCTC CATACCCACT CATTGGGCAT 
GAATATCCAT ATGGAATTAA AATGAGCCAT CTTCGAGACT CTGCACTCCT GACACCGGGA 
TCAAAGGACT CTACCACCCC TTTCAACCTC CAGGAGCCCT TCCTTATGGA ACAGCTCCCC 
CGAGAGATGC AGTGCCAGTT CATCCTGAAG CCCAGCCGCC TGGCTGCAGC CCAGCAACTG 
AGTGATTCAG GTCATAAGAC ATTTAAAAGG AGAAGATCTA TCATAAACTG GGCCTTCTGG 
CGAGGTTCTA GCACTCACCT GGACAACTTG CCCTCATCGC CAACATCACC TATGCCAGGA 
CAGCTCTTTG GAATTTCTCT GCCAAATATT TGTGAGAATG ACAATCTGCC CAAACCTGTC 
TTGGATATGC TTTTCTTTCT TAATCAAAAA GGACCTCTCA CCAAAGGTAT CTTCAGGCAA 
TCAGCCAATG TGAAATCCTG CAGAGAACTA AAAGAGAAAT TGAATTCTGG AGTCGAAGTA 
CACCTAGACT GTGAATCTAT TTTTGTGATA GCATCTGTCT TAAAGGATTT TCTGCGAAAT 
ATTCCAGGAA GTATTTTTTC ATCAGATCTC TATGATCACT GGGTCTCTGT AATGGATCAA 
GGAAATGATG AAGAGAAAAT AAATACTGTT CAAAGGCTAT TAGACCAGCT TCCGAGAGCC 
AATGTTGTTC TCCTAAGGTA TCTTTTTGGG GTGTTACACA ACATTGAGCA ACATTCCTCA 
TCCAATCAGA TGACTGCATT TAATTTAGCT GTGTGTGTCG CTCCAAGTAT TCTTTGGCCT 
CCTGCTTCCT CCAGCCCAGA ACTAGAAAAC GAATTTACAA AAAAGGTTTC CCTGCTTATA 
CAATTTCTGA TTGAGAATTG CCTTAGGATA TTTGGAGAAG AAATCACTTC CCTCTTCAGA 
GAGGTTTCAG TGAGATGTGA CACTAGAGAG AATGCCTCAG ATATTTCTTG CTTTCAACTG 
AATGACTCCT CCTATGACAG CTTGGAAAAT GAGCTAAATG AGGATGTTGA TGCACCATGC 
AGTGACTTGG TAAAGAAACT TGGCCAGGGG AGCAGAAGCA TGGACTCTGT CTTAACCCTC 
AGTGACTATG ATCTTGACCA GCCCGAGGTG GAAGGCCTTT TAACCCTAAG CGACTTTGAC 
TTGGCCCATT CTAAAGATGA AGATGTTCAA ATGAAACGGC CTCTTGAATC CAAGCCGGTG 
AACATTTTAG TGTACACAAA GATCCCACTG CGGGATCATG CCAGGGCCCC ATCTGCCATG 
TGCACACCCA GCTACCTGTC CACAGCTGCA GCAAATGCTG CAAAAAGCCT GAGGCGACAC 
CGGCGTTGCT CAGAGCCCAG CATCGACTAT CTGGATTCAA AGCTTTCCTA CCTCAGGGAG 
TTTTATCAGA AAAAGCTACG CAAGTCCAGC TGTGATGCAA TTCTTTCTCA AAAAGATGAA 
GACTATCTGA AGCAGAATCA ACCCCTCCAG GAGGAAGGAA AGACATGTTT TAAACAGAGT 
TTAGTCACAG GCACTGATGT CAGCAAGAAA AATGCCACTA CTCAAAACAC TAAGAAGAAA 
AGCTTGTCTG GTAGTGAAGG AAATCACGTG AAACTTTTCC CTAAGTCTAA GCCAGTGGCC 
ATTTCTGTGG CATCTTATAG TCCTATGTCC TCACAGGATC ATTCCAAGAA CCAGCCCTTT 
GATGTGAATA CATCTGGATA CTCCCCACCA CACACAGCAG ATGCCCTCAA GGGTCCAAGG 
ACACATCGGC GCTGCTCAGA GCCCAACATA GAAGACCAGA ACCGCAAGCT GACCTATCTC 
AGGGGAATTT ATTCAAAGAA ACAACATAAA ACCAGCTGTG AAGCTGGTCT CTTGCATGGA 
GAGGAGGATT ATCTCAAACG GCATAAGTCT TTGCAAATGG AGGGGCAGAA GCTCATTAAT 
CAGAGTTTAG TCATGGGGAT TGAGGTGGGC AAGAGTAGTG CCACAAACCA AAACACTGAG 
AAGGTTTTAC CCCCAAGATT AAACCTTTGC CCAAGGACCA GCTATTCCAG CTTATCCTCC 
CCAGGCACTT CCCCATCCGG CTCATCAGTA AGCTCCCAAG ACAGTGCTTT TTCTCAGATT 
TCTGAACACT CTGTGTTTAC ACCCACTGAG ACTTCCTCTC CAATAGATTG CACTTTTCAG 
GCTCAGAGAA AACGGGAAGA CCTTTCTCCT GACTTTAGCA ATGCCAGCCA TGTTTCCGGA 
ATGCCCGGTC CCTCATCAGG GCAGGCTTGC AGCCGCCCAG CCTATACAAA GAAGGACACC 
ATGGAGTGGC ATTCACAAAT GCATTCTGTA ACTCTTCATC CCAGCACATG GTTGAGAAAT 
GGTGTGGCCA GTTTGAAAAA CTGGTCCCTC AAAAAGAAAG CAAAGGCAGC CAGACCAGAG 
GAAGAGAAAA TAGCTTCTCC AAAAGGACCC TTAGAGCCAC CCCCACATGC TTCTGGTGTT 
CCAGAAGCCA ACTCACTGCA AGAGGAACAA AAAGACTTGC CCTTAAGGGC AGCTGAAGGA 
CTGTCCCCTG TGCAGTCAGC CCAAAGGTGT AGTTCTTCTC CCTTCCAGGA CTCAGAGAGA 
CACTGTAGCT CTCCATTCAG CCTGGTGGAG AGCAGACTTA AGCTGTGCAT GAAGTCACAT 
GAGGAAATAG AGCCTGGTAG TCAGAGCTCT TCTGGTTCTC TGCCTTGGGA AAGAGCCTCA 
GCCAGCTCTT GGACTCTAGA GGATGCGACC AGCCCAGACT CAGGGCCTAC AGTGGTCTGC 
GACATTGAGG ACAGGTATTT AACCAAAGAC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP20 ( NM_001258415.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001245344.1 Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 2, mRNA.

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CloneID	OHu12330
Clone ID Related Accession (Same CDS sequence)	NM_001258415.2 , NM_001258415.1
Accession Version	NM_001258415.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 20 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY496266.1 and AP001883.6. On Jun 2, 2019 this sequence version replaced NM_001258415.1. Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY496266.1, SRR1803616.252833.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCCC GGGAGCGGCA GCCGGCGCTG AAGAAGAAAA TGAAAACACT AGCAGAAAGG 
AGGAGGAGCG CTCCATCTCT TATCCTGGAT AAAGCCCTAC AAAAACGGCC TACTACCAGG 
GACAGTCCTT CTGCTAGTGT TGACACATGC ACATTTCTGT CATCATTAGT GTGCTCCAAT 
AGGACTCTGC TGATTGATGG CCGGGCAGAA CTCAAAAGAG GCCTCCAGAG GCAGGAGCGG 
CATCTTTTCC TATTCAATGA TCTGTTTGTT GTGGCCAAAA TCAAATATAA CAATAACTTT 
AAGATAAAAA ATAAAATTAA ATTAACTGAT ATGTGGACAG CAAGCTGTGT GGATGAAGTG 
GGAGAAGGCA ACACCAATGC CATGAAATCC TTTGTTTTGG GCTGGCCCAC AGTGAACTTT 
GTGGCCACTT TCAGTTCTCC AGAACAAAAG GACAAATGGC TCTCTCTCCT TCAGAGATAC 
ATCAATCTAG AGAAAGAAAA GGACTACCCG AAGAGCATTC CCCTCAAAAT CTTCGCCAAG 
GACATTGGGA ATTGTGCCTA CTCTAAAACT ATAACAGTAA TGAATTCAGA TACAGCGAAT 
GAAGTTATCA ACATGTCATT ACCAATGCTA GGGATAACTG GCTCTGAGAG AGATTACCAG 
TTGTGGGTCA ATTCTGGCAA AGAAGAGGCT CCATACCCAC TCATTGGGCA TGAATATCCA 
TATGGAATTA AAATGAGCCA TCTTCGAGAC TCTGCACTCC TGACACCGGG ATCAAAGGAC 
TCTACCACCC CTTTCAACCT CCAGGAGCCC TTCCTTATGG AACAGCTCCC CCGAGAGATG 
CAGTGCCAGT TCATCCTGAA GCCCAGCCGC CTGGCTGCAG CCCAGCAACT GAGTGATTCA 
GGTCATAAGA CATTTAAAAG GAGAAGATCT ATCATAAACT GGGCCTTCTG GCGAGGTTCT 
AGCACTCACC TGGACAACTT GCCCTCATCG CCAACATCAC CTATGCCAGG ACAGCTCTTT 
GGAATTTCTC TGCCAAATAT TTGTGAGAAT GACAATCTGC CCAAACCTGT CTTGGATATG 
CTTTTCTTTC TTAATCAAAA AGGACCTCTC ACCAAAGGTA TCTTCAGGCA ATCAGCCAAT 
GTGAAATCCT GCAGAGAACT AAAAGAGAAA TTGAATTCTG GAGTCGAAGT ACACCTAGAC 
TGTGAATCTA TTTTTGTGAT AGCATCTGTC TTAAAGGATT TTCTGCGAAA TATTCCAGGA 
AGTATTTTTT CATCAGATCT CTATGATCAC TGGGTCTCTG TAATGGATCA AGGAAATGAT 
GAAGAGAAAA TAAATACTGT TCAAAGGCTA TTAGACCAGC TTCCGAGAGC CAATGTTGTT 
CTCCTAAGGT ATCTTTTTGG GGTGTTACAC AACATTGAGC AACATTCCTC ATCCAATCAG 
ATGACTGCAT TTAATTTAGC TGTGTGTGTC GCTCCAAGTA TTCTTTGGCC TCCTGCTTCC 
TCCAGCCCAG AACTAGAAAA CGAATTTACA AAAAAGGTTT CCCTGCTTAT ACAATTTCTG 
ATTGAGAATT GCCTTAGGAT ATTTGGAGAA GAAATCACTT CCCTCTTCAG AGAGGTTTCA 
GTGAGATGTG ACACTAGAGA GAATGCCTCA GATATTTCTT GCTTTCAACT GAATGACTCC 
TCCTATGACA GCTTGGAAAA TGAGCTAAAT GAGGATGTTG ATGCACCATG CAGTGACTTG 
GTAAAGAAAC TTGGCCAGGG GAGCAGAAGC ATGGACTCTG TCTTAACCCT CAGTGACTAT 
GATCTTGACC AGCCCGAGGT GGAAGGCCTT TTAACCCTAA GCGACTTTGA CTTGGCCCAT 
TCTAAAGATG AAGATGTTCA AATGAAACGG CCTCTTGAAT CCAAGCCGGT GAACATTTTA 
GTGTACACAA AGATCCCACT GCGGGATCAT GCCAGGGCCC CATCTGCCAT GTGCACACCC 
AGCTACCTGT CCACAGCTGC AGCAAATGCT GCAAAAAGCC TGAGGCGACA CCGGCGTTGC 
TCAGAGCCCA GCATCGACTA TCTGGATTCA AAGCTTTCCT ACCTCAGGGA GTTTTATCAG 
AAAAAGCTAC GCAAGTCCAG CTGTGATGCA ATTCTTTCTC AAAAAGATGA AGACTATCTG 
AAGCAGAATC AACCCCTCCA GGAGGAAGGA AAGACATGTT TTAAACAGAG TTTAGTCACA 
GGCACTGATG TCAGCAAGAA AAATGCCACT ACTCAAAACA CTAAGAAGAA AAGCTTGTCT 
GGTAGTGAAG GAAATCACGT GAAACTTTTC CCTAAGTCTA AGCCAGTGGC CATTTCTGTG 
GCATCTTATA GTCCTATGTC CTCACAGGAT CATTCCAAGA ACCAGCCCTT TGATGTGAAT 
ACATCTGGAT ACTCCCCACC ACACACAGCA GATGCCCTCA AGGGTCCAAG GACACATCGG 
CGCTGCTCAG AGCCCAACAT AGAAGACCAG AACCGCAAGC TGACCTATCT CAGGGGAATT 
TATTCAAAGA AACAACATAA AACCAGCTGT GAAGCTGGTC TCTTGCATGG AGAGGAGGAT 
TATCTCAAAC GGCATAAGTC TTTGCAAATG GAGGGGCAGA AGCTCATTAA TCAGAGTTTA 
GTCATGGGGA TTGAGGTGGG CAAGAGTAGT GCCACAAACC AAAACACTGA GAAGGTTTTA 
CCCCCAAGAT TAAACCTTTG CCCAAGGACC AGCTATTCCA GCTTATCCTC CCCAGGCACT 
TCCCCATCCG GCTCATCAGT AAGCTCCCAA GACAGTGCTT TTTCTCAGAT TTCTGAACAC 
TCTGTGTTTA CACCCACTGA GACTTCCTCT CCAATAGATT GCACTTTTCA GGCTCAGAGA 
AAACGGGAAG ACCTTTCTCC TGACTTTAGC AATGCCAGCC ATGTTTCCGG AATGCCCGGT 
CCCTCATCAG GGCAGGCTTG CAGCCGCCCA GCCTATACAA AGAAGGACAC CATGGAGTGG 
CATTCACAAA TGCATTCTGT AACTCTTCAT CCCAGCACAT GGTTGAGAAA TGGTGTGGCC 
AGTTTGAAAA ACTGGTCCCT CAAAAAGAAA GCAAAGGCAG CCAGACCAGA GGAAGAGAAA 
ATAGCTTCTC CAAAAGGACC CTTAGAGCCA CCCCCACATG CTTCTGGTGT TCCAGAAGCC 
AACTCACTGC AAGAGGAACA AAAAGACTTG CCCTTAAGGG CAGCTGAAGG ACTGTCCCCT 
GTGCAGTCAG CCCAAAGGTG TAGTTCTTCT CCCTTCCAGG ACTCAGAGAG ACACTGTAGC 
TCTCCATTCA GCCTGGTGGA GAGCAGACTT AAGCTGTGCA TGAAGTCACA TGAGGAAATA 
GAGCCTGGTA GTCAGAGCTC TTCTGGTTCT CTGCCTTGGG AAAGAGCCTC AGCCAGCTCT 
TGGACTCTAG AGGATGCGAC CAGCCCAGAC TCAGGGCCTA CAGTGGTCTG CGACATTGAG 
GACAGGTATT TAACCAAAGA CATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245344.1
CDS	133..3639
Translation	MSARERQPALKKKMKTLAERRRSAPSLILDKALQKRPTTRDSPSASVDTCTFLSSLVCSNRTLLIDGRAELKRGLQRQERHLFLFNDLFVVAKIKYNNNFKIKNKIKLTDMWTASCVDEVGEGNTNAMKSFVLGWPTVNFVATFSSPEQKDKWLSLLQRYINLEKEKDYPKSIPLKIFAKDIGNCAYSKTITVMNSDTANEVINMSLPMLGITGSERDYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDSALLTPGSKDSTTPFNLQEPFLMEQLPREMQCQFILKPSRLAAAQQLSDSGHKTFKRRRSIINWAFWRGSSTHLDNLPSSPTSPMPGQLFGISLPNICENDNLPKPVLDMLFFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGVEVHLDCESIFVIASVLKDFLRNIPGSIFSSDLYDHWVSVMDQGNDEEKINTVQRLLDQLPRANVVLLRYLFGVLHNIEQHSSSNQMTAFNLAVCVAPSILWPPASSSPELENEFTKKVSLLIQFLIENCLRIFGEEITSLFREVSVRCDTRENASDISCFQLNDSSYDSLENELNEDVDAPCSDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLAHSKDEDVQMKRPLESKPVNILVYTKIPLRDHARAPSAMCTPSYLSTAAANAAKSLRRHRRCSEPSIDYLDSKLSYLREFYQKKLRKSSCDAILSQKDEDYLKQNQPLQEEGKTCFKQSLVTGTDVSKKNATTQNTKKKSLSGSEGNHVKLFPKSKPVAISVASYSPMSSQDHSKNQPFDVNTSGYSPPHTADALKGPRTHRRCSEPNIEDQNRKLTYLRGIYSKKQHKTSCEAGLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNTEKVLPPRLNLCPRTSYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQAQRKREDLSPDFSNASHVSGMPGPSSGQACSRPAYTKKDTMEWHSQMHSVTLHPSTWLRNGVASLKNWSLKKKAKAARPEEEKIASPKGPLEPPPHASGVPEANSLQEEQKDLPLRAAEGLSPVQSAQRCSSSPFQDSERHCSSPFSLVESRLKLCMKSHEEIEPGSQSSSGSLPWERASASSWTLEDATSPDSGPTVVCDIEDRYLTKDI

Target ORF information:

RefSeq Version	NM_001258415.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258415.2

ORF Insert Sequence:

ATGTCTGCCC GGGAGCGGCA GCCGGCGCTG AAGAAGAAAA TGAAAACACT AGCAGAAAGG 
AGGAGGAGCG CTCCATCTCT TATCCTGGAT AAAGCCCTAC AAAAACGGCC TACTACCAGG 
GACAGTCCTT CTGCTAGTGT TGACACATGC ACATTTCTGT CATCATTAGT GTGCTCCAAT 
AGGACTCTGC TGATTGATGG CCGGGCAGAA CTCAAAAGAG GCCTCCAGAG GCAGGAGCGG 
CATCTTTTCC TATTCAATGA TCTGTTTGTT GTGGCCAAAA TCAAATATAA CAATAACTTT 
AAGATAAAAA ATAAAATTAA ATTAACTGAT ATGTGGACAG CAAGCTGTGT GGATGAAGTG 
GGAGAAGGCA ACACCAATGC CATGAAATCC TTTGTTTTGG GCTGGCCCAC AGTGAACTTT 
GTGGCCACTT TCAGTTCTCC AGAACAAAAG GACAAATGGC TCTCTCTCCT TCAGAGATAC 
ATCAATCTAG AGAAAGAAAA GGACTACCCG AAGAGCATTC CCCTCAAAAT CTTCGCCAAG 
GACATTGGGA ATTGTGCCTA CTCTAAAACT ATAACAGTAA TGAATTCAGA TACAGCGAAT 
GAAGTTATCA ACATGTCATT ACCAATGCTA GGGATAACTG GCTCTGAGAG AGATTACCAG 
TTGTGGGTCA ATTCTGGCAA AGAAGAGGCT CCATACCCAC TCATTGGGCA TGAATATCCA 
TATGGAATTA AAATGAGCCA TCTTCGAGAC TCTGCACTCC TGACACCGGG ATCAAAGGAC 
TCTACCACCC CTTTCAACCT CCAGGAGCCC TTCCTTATGG AACAGCTCCC CCGAGAGATG 
CAGTGCCAGT TCATCCTGAA GCCCAGCCGC CTGGCTGCAG CCCAGCAACT GAGTGATTCA 
GGTCATAAGA CATTTAAAAG GAGAAGATCT ATCATAAACT GGGCCTTCTG GCGAGGTTCT 
AGCACTCACC TGGACAACTT GCCCTCATCG CCAACATCAC CTATGCCAGG ACAGCTCTTT 
GGAATTTCTC TGCCAAATAT TTGTGAGAAT GACAATCTGC CCAAACCTGT CTTGGATATG 
CTTTTCTTTC TTAATCAAAA AGGACCTCTC ACCAAAGGTA TCTTCAGGCA ATCAGCCAAT 
GTGAAATCCT GCAGAGAACT AAAAGAGAAA TTGAATTCTG GAGTCGAAGT ACACCTAGAC 
TGTGAATCTA TTTTTGTGAT AGCATCTGTC TTAAAGGATT TTCTGCGAAA TATTCCAGGA 
AGTATTTTTT CATCAGATCT CTATGATCAC TGGGTCTCTG TAATGGATCA AGGAAATGAT 
GAAGAGAAAA TAAATACTGT TCAAAGGCTA TTAGACCAGC TTCCGAGAGC CAATGTTGTT 
CTCCTAAGGT ATCTTTTTGG GGTGTTACAC AACATTGAGC AACATTCCTC ATCCAATCAG 
ATGACTGCAT TTAATTTAGC TGTGTGTGTC GCTCCAAGTA TTCTTTGGCC TCCTGCTTCC 
TCCAGCCCAG AACTAGAAAA CGAATTTACA AAAAAGGTTT CCCTGCTTAT ACAATTTCTG 
ATTGAGAATT GCCTTAGGAT ATTTGGAGAA GAAATCACTT CCCTCTTCAG AGAGGTTTCA 
GTGAGATGTG ACACTAGAGA GAATGCCTCA GATATTTCTT GCTTTCAACT GAATGACTCC 
TCCTATGACA GCTTGGAAAA TGAGCTAAAT GAGGATGTTG ATGCACCATG CAGTGACTTG 
GTAAAGAAAC TTGGCCAGGG GAGCAGAAGC ATGGACTCTG TCTTAACCCT CAGTGACTAT 
GATCTTGACC AGCCCGAGGT GGAAGGCCTT TTAACCCTAA GCGACTTTGA CTTGGCCCAT 
TCTAAAGATG AAGATGTTCA AATGAAACGG CCTCTTGAAT CCAAGCCGGT GAACATTTTA 
GTGTACACAA AGATCCCACT GCGGGATCAT GCCAGGGCCC CATCTGCCAT GTGCACACCC 
AGCTACCTGT CCACAGCTGC AGCAAATGCT GCAAAAAGCC TGAGGCGACA CCGGCGTTGC 
TCAGAGCCCA GCATCGACTA TCTGGATTCA AAGCTTTCCT ACCTCAGGGA GTTTTATCAG 
AAAAAGCTAC GCAAGTCCAG CTGTGATGCA ATTCTTTCTC AAAAAGATGA AGACTATCTG 
AAGCAGAATC AACCCCTCCA GGAGGAAGGA AAGACATGTT TTAAACAGAG TTTAGTCACA 
GGCACTGATG TCAGCAAGAA AAATGCCACT ACTCAAAACA CTAAGAAGAA AAGCTTGTCT 
GGTAGTGAAG GAAATCACGT GAAACTTTTC CCTAAGTCTA AGCCAGTGGC CATTTCTGTG 
GCATCTTATA GTCCTATGTC CTCACAGGAT CATTCCAAGA ACCAGCCCTT TGATGTGAAT 
ACATCTGGAT ACTCCCCACC ACACACAGCA GATGCCCTCA AGGGTCCAAG GACACATCGG 
CGCTGCTCAG AGCCCAACAT AGAAGACCAG AACCGCAAGC TGACCTATCT CAGGGGAATT 
TATTCAAAGA AACAACATAA AACCAGCTGT GAAGCTGGTC TCTTGCATGG AGAGGAGGAT 
TATCTCAAAC GGCATAAGTC TTTGCAAATG GAGGGGCAGA AGCTCATTAA TCAGAGTTTA 
GTCATGGGGA TTGAGGTGGG CAAGAGTAGT GCCACAAACC AAAACACTGA GAAGGTTTTA 
CCCCCAAGAT TAAACCTTTG CCCAAGGACC AGCTATTCCA GCTTATCCTC CCCAGGCACT 
TCCCCATCCG GCTCATCAGT AAGCTCCCAA GACAGTGCTT TTTCTCAGAT TTCTGAACAC 
TCTGTGTTTA CACCCACTGA GACTTCCTCT CCAATAGATT GCACTTTTCA GGCTCAGAGA 
AAACGGGAAG ACCTTTCTCC TGACTTTAGC AATGCCAGCC ATGTTTCCGG AATGCCCGGT 
CCCTCATCAG GGCAGGCTTG CAGCCGCCCA GCCTATACAA AGAAGGACAC CATGGAGTGG 
CATTCACAAA TGCATTCTGT AACTCTTCAT CCCAGCACAT GGTTGAGAAA TGGTGTGGCC 
AGTTTGAAAA ACTGGTCCCT CAAAAAGAAA GCAAAGGCAG CCAGACCAGA GGAAGAGAAA 
ATAGCTTCTC CAAAAGGACC CTTAGAGCCA CCCCCACATG CTTCTGGTGT TCCAGAAGCC 
AACTCACTGC AAGAGGAACA AAAAGACTTG CCCTTAAGGG CAGCTGAAGG ACTGTCCCCT 
GTGCAGTCAG CCCAAAGGTG TAGTTCTTCT CCCTTCCAGG ACTCAGAGAG ACACTGTAGC 
TCTCCATTCA GCCTGGTGGA GAGCAGACTT AAGCTGTGCA TGAAGTCACA TGAGGAAATA 
GAGCCTGGTA GTCAGAGCTC TTCTGGTTCT CTGCCTTGGG AAAGAGCCTC AGCCAGCTCT 
TGGACTCTAG AGGATGCGAC CAGCCCAGAC TCAGGGCCTA CAGTGGTCTG CGACATTGAG 
GACAGGTATT TAACCAAAGA CATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP20 ( NM_001258415.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001245344.1 Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12330
Clone ID Related Accession (Same CDS sequence)	NM_001258415.2 , NM_001258415.1
Accession Version	NM_001258415.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 20 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY496266.1 and AP001883.6. Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY496266.1, SRR1803616.252833.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCCC GGGAGCGGCA GCCGGCGCTG AAGAAGAAAA TGAAAACACT AGCAGAAAGG 
AGGAGGAGCG CTCCATCTCT TATCCTGGAT AAAGCCCTAC AAAAACGGCC TACTACCAGG 
GACAGTCCTT CTGCTAGTGT TGACACATGC ACATTTCTGT CATCATTAGT GTGCTCCAAT 
AGGACTCTGC TGATTGATGG CCGGGCAGAA CTCAAAAGAG GCCTCCAGAG GCAGGAGCGG 
CATCTTTTCC TATTCAATGA TCTGTTTGTT GTGGCCAAAA TCAAATATAA CAATAACTTT 
AAGATAAAAA ATAAAATTAA ATTAACTGAT ATGTGGACAG CAAGCTGTGT GGATGAAGTG 
GGAGAAGGCA ACACCAATGC CATGAAATCC TTTGTTTTGG GCTGGCCCAC AGTGAACTTT 
GTGGCCACTT TCAGTTCTCC AGAACAAAAG GACAAATGGC TCTCTCTCCT TCAGAGATAC 
ATCAATCTAG AGAAAGAAAA GGACTACCCG AAGAGCATTC CCCTCAAAAT CTTCGCCAAG 
GACATTGGGA ATTGTGCCTA CTCTAAAACT ATAACAGTAA TGAATTCAGA TACAGCGAAT 
GAAGTTATCA ACATGTCATT ACCAATGCTA GGGATAACTG GCTCTGAGAG AGATTACCAG 
TTGTGGGTCA ATTCTGGCAA AGAAGAGGCT CCATACCCAC TCATTGGGCA TGAATATCCA 
TATGGAATTA AAATGAGCCA TCTTCGAGAC TCTGCACTCC TGACACCGGG ATCAAAGGAC 
TCTACCACCC CTTTCAACCT CCAGGAGCCC TTCCTTATGG AACAGCTCCC CCGAGAGATG 
CAGTGCCAGT TCATCCTGAA GCCCAGCCGC CTGGCTGCAG CCCAGCAACT GAGTGATTCA 
GGTCATAAGA CATTTAAAAG GAGAAGATCT ATCATAAACT GGGCCTTCTG GCGAGGTTCT 
AGCACTCACC TGGACAACTT GCCCTCATCG CCAACATCAC CTATGCCAGG ACAGCTCTTT 
GGAATTTCTC TGCCAAATAT TTGTGAGAAT GACAATCTGC CCAAACCTGT CTTGGATATG 
CTTTTCTTTC TTAATCAAAA AGGACCTCTC ACCAAAGGTA TCTTCAGGCA ATCAGCCAAT 
GTGAAATCCT GCAGAGAACT AAAAGAGAAA TTGAATTCTG GAGTCGAAGT ACACCTAGAC 
TGTGAATCTA TTTTTGTGAT AGCATCTGTC TTAAAGGATT TTCTGCGAAA TATTCCAGGA 
AGTATTTTTT CATCAGATCT CTATGATCAC TGGGTCTCTG TAATGGATCA AGGAAATGAT 
GAAGAGAAAA TAAATACTGT TCAAAGGCTA TTAGACCAGC TTCCGAGAGC CAATGTTGTT 
CTCCTAAGGT ATCTTTTTGG GGTGTTACAC AACATTGAGC AACATTCCTC ATCCAATCAG 
ATGACTGCAT TTAATTTAGC TGTGTGTGTC GCTCCAAGTA TTCTTTGGCC TCCTGCTTCC 
TCCAGCCCAG AACTAGAAAA CGAATTTACA AAAAAGGTTT CCCTGCTTAT ACAATTTCTG 
ATTGAGAATT GCCTTAGGAT ATTTGGAGAA GAAATCACTT CCCTCTTCAG AGAGGTTTCA 
GTGAGATGTG ACACTAGAGA GAATGCCTCA GATATTTCTT GCTTTCAACT GAATGACTCC 
TCCTATGACA GCTTGGAAAA TGAGCTAAAT GAGGATGTTG ATGCACCATG CAGTGACTTG 
GTAAAGAAAC TTGGCCAGGG GAGCAGAAGC ATGGACTCTG TCTTAACCCT CAGTGACTAT 
GATCTTGACC AGCCCGAGGT GGAAGGCCTT TTAACCCTAA GCGACTTTGA CTTGGCCCAT 
TCTAAAGATG AAGATGTTCA AATGAAACGG CCTCTTGAAT CCAAGCCGGT GAACATTTTA 
GTGTACACAA AGATCCCACT GCGGGATCAT GCCAGGGCCC CATCTGCCAT GTGCACACCC 
AGCTACCTGT CCACAGCTGC AGCAAATGCT GCAAAAAGCC TGAGGCGACA CCGGCGTTGC 
TCAGAGCCCA GCATCGACTA TCTGGATTCA AAGCTTTCCT ACCTCAGGGA GTTTTATCAG 
AAAAAGCTAC GCAAGTCCAG CTGTGATGCA ATTCTTTCTC AAAAAGATGA AGACTATCTG 
AAGCAGAATC AACCCCTCCA GGAGGAAGGA AAGACATGTT TTAAACAGAG TTTAGTCACA 
GGCACTGATG TCAGCAAGAA AAATGCCACT ACTCAAAACA CTAAGAAGAA AAGCTTGTCT 
GGTAGTGAAG GAAATCACGT GAAACTTTTC CCTAAGTCTA AGCCAGTGGC CATTTCTGTG 
GCATCTTATA GTCCTATGTC CTCACAGGAT CATTCCAAGA ACCAGCCCTT TGATGTGAAT 
ACATCTGGAT ACTCCCCACC ACACACAGCA GATGCCCTCA AGGGTCCAAG GACACATCGG 
CGCTGCTCAG AGCCCAACAT AGAAGACCAG AACCGCAAGC TGACCTATCT CAGGGGAATT 
TATTCAAAGA AACAACATAA AACCAGCTGT GAAGCTGGTC TCTTGCATGG AGAGGAGGAT 
TATCTCAAAC GGCATAAGTC TTTGCAAATG GAGGGGCAGA AGCTCATTAA TCAGAGTTTA 
GTCATGGGGA TTGAGGTGGG CAAGAGTAGT GCCACAAACC AAAACACTGA GAAGGTTTTA 
CCCCCAAGAT TAAACCTTTG CCCAAGGACC AGCTATTCCA GCTTATCCTC CCCAGGCACT 
TCCCCATCCG GCTCATCAGT AAGCTCCCAA GACAGTGCTT TTTCTCAGAT TTCTGAACAC 
TCTGTGTTTA CACCCACTGA GACTTCCTCT CCAATAGATT GCACTTTTCA GGCTCAGAGA 
AAACGGGAAG ACCTTTCTCC TGACTTTAGC AATGCCAGCC ATGTTTCCGG AATGCCCGGT 
CCCTCATCAG GGCAGGCTTG CAGCCGCCCA GCCTATACAA AGAAGGACAC CATGGAGTGG 
CATTCACAAA TGCATTCTGT AACTCTTCAT CCCAGCACAT GGTTGAGAAA TGGTGTGGCC 
AGTTTGAAAA ACTGGTCCCT CAAAAAGAAA GCAAAGGCAG CCAGACCAGA GGAAGAGAAA 
ATAGCTTCTC CAAAAGGACC CTTAGAGCCA CCCCCACATG CTTCTGGTGT TCCAGAAGCC 
AACTCACTGC AAGAGGAACA AAAAGACTTG CCCTTAAGGG CAGCTGAAGG ACTGTCCCCT 
GTGCAGTCAG CCCAAAGGTG TAGTTCTTCT CCCTTCCAGG ACTCAGAGAG ACACTGTAGC 
TCTCCATTCA GCCTGGTGGA GAGCAGACTT AAGCTGTGCA TGAAGTCACA TGAGGAAATA 
GAGCCTGGTA GTCAGAGCTC TTCTGGTTCT CTGCCTTGGG AAAGAGCCTC AGCCAGCTCT 
TGGACTCTAG AGGATGCGAC CAGCCCAGAC TCAGGGCCTA CAGTGGTCTG CGACATTGAG 
GACAGGTATT TAACCAAAGA CATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245344.1
CDS	358..3864
Translation	MSARERQPALKKKMKTLAERRRSAPSLILDKALQKRPTTRDSPSASVDTCTFLSSLVCSNRTLLIDGRAELKRGLQRQERHLFLFNDLFVVAKIKYNNNFKIKNKIKLTDMWTASCVDEVGEGNTNAMKSFVLGWPTVNFVATFSSPEQKDKWLSLLQRYINLEKEKDYPKSIPLKIFAKDIGNCAYSKTITVMNSDTANEVINMSLPMLGITGSERDYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDSALLTPGSKDSTTPFNLQEPFLMEQLPREMQCQFILKPSRLAAAQQLSDSGHKTFKRRRSIINWAFWRGSSTHLDNLPSSPTSPMPGQLFGISLPNICENDNLPKPVLDMLFFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGVEVHLDCESIFVIASVLKDFLRNIPGSIFSSDLYDHWVSVMDQGNDEEKINTVQRLLDQLPRANVVLLRYLFGVLHNIEQHSSSNQMTAFNLAVCVAPSILWPPASSSPELENEFTKKVSLLIQFLIENCLRIFGEEITSLFREVSVRCDTRENASDISCFQLNDSSYDSLENELNEDVDAPCSDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLAHSKDEDVQMKRPLESKPVNILVYTKIPLRDHARAPSAMCTPSYLSTAAANAAKSLRRHRRCSEPSIDYLDSKLSYLREFYQKKLRKSSCDAILSQKDEDYLKQNQPLQEEGKTCFKQSLVTGTDVSKKNATTQNTKKKSLSGSEGNHVKLFPKSKPVAISVASYSPMSSQDHSKNQPFDVNTSGYSPPHTADALKGPRTHRRCSEPNIEDQNRKLTYLRGIYSKKQHKTSCEAGLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNTEKVLPPRLNLCPRTSYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQAQRKREDLSPDFSNASHVSGMPGPSSGQACSRPAYTKKDTMEWHSQMHSVTLHPSTWLRNGVASLKNWSLKKKAKAARPEEEKIASPKGPLEPPPHASGVPEANSLQEEQKDLPLRAAEGLSPVQSAQRCSSSPFQDSERHCSSPFSLVESRLKLCMKSHEEIEPGSQSSSGSLPWERASASSWTLEDATSPDSGPTVVCDIEDRYLTKDI

Target ORF information:

RefSeq Version	NM_001258415.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258415.1

ORF Insert Sequence:

ATGTCTGCCC GGGAGCGGCA GCCGGCGCTG AAGAAGAAAA TGAAAACACT AGCAGAAAGG 
AGGAGGAGCG CTCCATCTCT TATCCTGGAT AAAGCCCTAC AAAAACGGCC TACTACCAGG 
GACAGTCCTT CTGCTAGTGT TGACACATGC ACATTTCTGT CATCATTAGT GTGCTCCAAT 
AGGACTCTGC TGATTGATGG CCGGGCAGAA CTCAAAAGAG GCCTCCAGAG GCAGGAGCGG 
CATCTTTTCC TATTCAATGA TCTGTTTGTT GTGGCCAAAA TCAAATATAA CAATAACTTT 
AAGATAAAAA ATAAAATTAA ATTAACTGAT ATGTGGACAG CAAGCTGTGT GGATGAAGTG 
GGAGAAGGCA ACACCAATGC CATGAAATCC TTTGTTTTGG GCTGGCCCAC AGTGAACTTT 
GTGGCCACTT TCAGTTCTCC AGAACAAAAG GACAAATGGC TCTCTCTCCT TCAGAGATAC 
ATCAATCTAG AGAAAGAAAA GGACTACCCG AAGAGCATTC CCCTCAAAAT CTTCGCCAAG 
GACATTGGGA ATTGTGCCTA CTCTAAAACT ATAACAGTAA TGAATTCAGA TACAGCGAAT 
GAAGTTATCA ACATGTCATT ACCAATGCTA GGGATAACTG GCTCTGAGAG AGATTACCAG 
TTGTGGGTCA ATTCTGGCAA AGAAGAGGCT CCATACCCAC TCATTGGGCA TGAATATCCA 
TATGGAATTA AAATGAGCCA TCTTCGAGAC TCTGCACTCC TGACACCGGG ATCAAAGGAC 
TCTACCACCC CTTTCAACCT CCAGGAGCCC TTCCTTATGG AACAGCTCCC CCGAGAGATG 
CAGTGCCAGT TCATCCTGAA GCCCAGCCGC CTGGCTGCAG CCCAGCAACT GAGTGATTCA 
GGTCATAAGA CATTTAAAAG GAGAAGATCT ATCATAAACT GGGCCTTCTG GCGAGGTTCT 
AGCACTCACC TGGACAACTT GCCCTCATCG CCAACATCAC CTATGCCAGG ACAGCTCTTT 
GGAATTTCTC TGCCAAATAT TTGTGAGAAT GACAATCTGC CCAAACCTGT CTTGGATATG 
CTTTTCTTTC TTAATCAAAA AGGACCTCTC ACCAAAGGTA TCTTCAGGCA ATCAGCCAAT 
GTGAAATCCT GCAGAGAACT AAAAGAGAAA TTGAATTCTG GAGTCGAAGT ACACCTAGAC 
TGTGAATCTA TTTTTGTGAT AGCATCTGTC TTAAAGGATT TTCTGCGAAA TATTCCAGGA 
AGTATTTTTT CATCAGATCT CTATGATCAC TGGGTCTCTG TAATGGATCA AGGAAATGAT 
GAAGAGAAAA TAAATACTGT TCAAAGGCTA TTAGACCAGC TTCCGAGAGC CAATGTTGTT 
CTCCTAAGGT ATCTTTTTGG GGTGTTACAC AACATTGAGC AACATTCCTC ATCCAATCAG 
ATGACTGCAT TTAATTTAGC TGTGTGTGTC GCTCCAAGTA TTCTTTGGCC TCCTGCTTCC 
TCCAGCCCAG AACTAGAAAA CGAATTTACA AAAAAGGTTT CCCTGCTTAT ACAATTTCTG 
ATTGAGAATT GCCTTAGGAT ATTTGGAGAA GAAATCACTT CCCTCTTCAG AGAGGTTTCA 
GTGAGATGTG ACACTAGAGA GAATGCCTCA GATATTTCTT GCTTTCAACT GAATGACTCC 
TCCTATGACA GCTTGGAAAA TGAGCTAAAT GAGGATGTTG ATGCACCATG CAGTGACTTG 
GTAAAGAAAC TTGGCCAGGG GAGCAGAAGC ATGGACTCTG TCTTAACCCT CAGTGACTAT 
GATCTTGACC AGCCCGAGGT GGAAGGCCTT TTAACCCTAA GCGACTTTGA CTTGGCCCAT 
TCTAAAGATG AAGATGTTCA AATGAAACGG CCTCTTGAAT CCAAGCCGGT GAACATTTTA 
GTGTACACAA AGATCCCACT GCGGGATCAT GCCAGGGCCC CATCTGCCAT GTGCACACCC 
AGCTACCTGT CCACAGCTGC AGCAAATGCT GCAAAAAGCC TGAGGCGACA CCGGCGTTGC 
TCAGAGCCCA GCATCGACTA TCTGGATTCA AAGCTTTCCT ACCTCAGGGA GTTTTATCAG 
AAAAAGCTAC GCAAGTCCAG CTGTGATGCA ATTCTTTCTC AAAAAGATGA AGACTATCTG 
AAGCAGAATC AACCCCTCCA GGAGGAAGGA AAGACATGTT TTAAACAGAG TTTAGTCACA 
GGCACTGATG TCAGCAAGAA AAATGCCACT ACTCAAAACA CTAAGAAGAA AAGCTTGTCT 
GGTAGTGAAG GAAATCACGT GAAACTTTTC CCTAAGTCTA AGCCAGTGGC CATTTCTGTG 
GCATCTTATA GTCCTATGTC CTCACAGGAT CATTCCAAGA ACCAGCCCTT TGATGTGAAT 
ACATCTGGAT ACTCCCCACC ACACACAGCA GATGCCCTCA AGGGTCCAAG GACACATCGG 
CGCTGCTCAG AGCCCAACAT AGAAGACCAG AACCGCAAGC TGACCTATCT CAGGGGAATT 
TATTCAAAGA AACAACATAA AACCAGCTGT GAAGCTGGTC TCTTGCATGG AGAGGAGGAT 
TATCTCAAAC GGCATAAGTC TTTGCAAATG GAGGGGCAGA AGCTCATTAA TCAGAGTTTA 
GTCATGGGGA TTGAGGTGGG CAAGAGTAGT GCCACAAACC AAAACACTGA GAAGGTTTTA 
CCCCCAAGAT TAAACCTTTG CCCAAGGACC AGCTATTCCA GCTTATCCTC CCCAGGCACT 
TCCCCATCCG GCTCATCAGT AAGCTCCCAA GACAGTGCTT TTTCTCAGAT TTCTGAACAC 
TCTGTGTTTA CACCCACTGA GACTTCCTCT CCAATAGATT GCACTTTTCA GGCTCAGAGA 
AAACGGGAAG ACCTTTCTCC TGACTTTAGC AATGCCAGCC ATGTTTCCGG AATGCCCGGT 
CCCTCATCAG GGCAGGCTTG CAGCCGCCCA GCCTATACAA AGAAGGACAC CATGGAGTGG 
CATTCACAAA TGCATTCTGT AACTCTTCAT CCCAGCACAT GGTTGAGAAA TGGTGTGGCC 
AGTTTGAAAA ACTGGTCCCT CAAAAAGAAA GCAAAGGCAG CCAGACCAGA GGAAGAGAAA 
ATAGCTTCTC CAAAAGGACC CTTAGAGCCA CCCCCACATG CTTCTGGTGT TCCAGAAGCC 
AACTCACTGC AAGAGGAACA AAAAGACTTG CCCTTAAGGG CAGCTGAAGG ACTGTCCCCT 
GTGCAGTCAG CCCAAAGGTG TAGTTCTTCT CCCTTCCAGG ACTCAGAGAG ACACTGTAGC 
TCTCCATTCA GCCTGGTGGA GAGCAGACTT AAGCTGTGCA TGAAGTCACA TGAGGAAATA 
GAGCCTGGTA GTCAGAGCTC TTCTGGTTCT CTGCCTTGGG AAAGAGCCTC AGCCAGCTCT 
TGGACTCTAG AGGATGCGAC CAGCCCAGAC TCAGGGCCTA CAGTGGTCTG CGACATTGAG 
GACAGGTATT TAACCAAAGA CATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP20 ( XM_006718893.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718956.1 Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant X1, mRNA.

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CloneID	OHu08682
Clone ID Related Accession (Same CDS sequence)	XM_006718893.3 , NM_020809.3
Accession Version	XM_006718893.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718893.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCGA TGTCCCCCCA GCAGGAGACT CTAGGGGGAC AGCCGGGGCG CTCCTCTTCC 
CTGACAGGAG TGTCTCGGCT CGCGGGAGGC AGCTGCACCA AGAAGAAAAT GAAAACACTA 
GCAGAAAGGA GGAGGAGCGC TCCATCTCTT ATCCTGGATA AAGCCCTACA AAAACGGCCT 
ACTACCAGGG ACAGTCCTTC TGCTAGTGTT GACACATGCA CATTTCTGTC ATCATTAGTG 
TGCTCCAATA GGACTCTGCT GATTGATGGC CGGGCAGAAC TCAAAAGAGG CCTCCAGAGG 
CAGGAGCGGC ATCTTTTCCT ATTCAATGAT CTGTTTGTTG TGGCCAAAAT CAAATATAAC 
AATAACTTTA AGATAAAAAA TAAAATTAAA TTAACTGATA TGTGGACAGC AAGCTGTGTG 
GATGAAGTGG GAGAAGGCAA CACCAATGCC ATGAAATCCT TTGTTTTGGG CTGGCCCACA 
GTGAACTTTG TGGCCACTTT CAGTTCTCCA GAACAAAAGG ACAAATGGCT CTCTCTCCTT 
CAGAGATACA TCAATCTAGA GAAAGAAAAG GACTACCCGA AGAGCATTCC CCTCAAAATC 
TTCGCCAAGG ACATTGGGAA TTGTGCCTAC TCTAAAACTA TAACAGTAAT GAATTCAGAT 
ACAGCGAATG AAGTTATCAA CATGTCATTA CCAATGCTAG GGATAACTGG CTCTGAGAGA 
GATTACCAGT TGTGGGTCAA TTCTGGCAAA GAAGAGGCTC CATACCCACT CATTGGGCAT 
GAATATCCAT ATGGAATTAA AATGAGCCAT CTTCGAGACT CTGCACTCCT GACACCGGGA 
TCAAAGGACT CTACCACCCC TTTCAACCTC CAGGAGCCCT TCCTTATGGA ACAGCTCCCC 
CGAGAGATGC AGTGCCAGTT CATCCTGAAG CCCAGCCGCC TGGCTGCAGC CCAGCAACTG 
AGTGATTCAG GTCATAAGAC ATTTAAAAGG AGAAGATCTA TCATAAACTG GGCCTTCTGG 
CGAGGTTCTA GCACTCACCT GGACAACTTG CCCTCATCGC CAACATCACC TATGCCAGGA 
CAGCTCTTTG GAATTTCTCT GCCAAATATT TGTGAGAATG ACAATCTGCC CAAACCTGTC 
TTGGATATGC TTTTCTTTCT TAATCAAAAA GGACCTCTCA CCAAAGGTAT CTTCAGGCAA 
TCAGCCAATG TGAAATCCTG CAGAGAACTA AAAGAGAAAT TGAATTCTGG AGTCGAAGTA 
CACCTAGACT GTGAATCTAT TTTTGTGATA GCATCTGTCT TAAAGGATTT TCTGCGAAAT 
ATTCCAGGAA GTATTTTTTC ATCAGATCTC TATGATCACT GGGTCTCTGT AATGGATCAA 
GGAAATGATG AAGAGAAAAT AAATACTGTT CAAAGGCTAT TAGACCAGCT TCCGAGAGCC 
AATGTTGTTC TCCTAAGGTA TCTTTTTGGG GTGTTACACA ACATTGAGCA ACATTCCTCA 
TCCAATCAGA TGACTGCATT TAATTTAGCT GTGTGTGTCG CTCCAAGTAT TCTTTGGCCT 
CCTGCTTCCT CCAGCCCAGA ACTAGAAAAC GAATTTACAA AAAAGGTTTC CCTGCTTATA 
CAATTTCTGA TTGAGAATTG CCTTAGGATA TTTGGAGAAG AAATCACTTC CCTCTTCAGA 
GAGGTTTCAG TGAGATGTGA CACTAGAGAG AATGCCTCAG ATATTTCTTG CTTTCAACTG 
AATGACTCCT CCTATGACAG CTTGGAAAAT GAGCTAAATG AGGATGTTGA TGCACCATGC 
AGTGACTTGG TAAAGAAACT TGGCCAGGGG AGCAGAAGCA TGGACTCTGT CTTAACCCTC 
AGTGACTATG ATCTTGACCA GCCCGAGGTG GAAGGCCTTT TAACCCTAAG CGACTTTGAC 
TTGGCCCATT CTAAAGATGA AGATGTTCAA ATGAAACGGC CTCTTGAATC CAAGCCGGTG 
AACATTTTAG TGTACACAAA GATCCCACTG CGGGATCATG CCAGGGCCCC ATCTGCCATG 
TGCACACCCA GCTACCTGTC CACAGCTGCA GCAAATGCTG CAAAAAGCCT GAGGCGACAC 
CGGCGTTGCT CAGAGCCCAG CATCGACTAT CTGGATTCAA AGCTTTCCTA CCTCAGGGAG 
TTTTATCAGA AAAAGCTACG CAAGTCCAGC TGTGATGCAA TTCTTTCTCA AAAAGATGAA 
GACTATCTGA AGCAGAATCA ACCCCTCCAG GAGGAAGGAA AGACATGTTT TAAACAGAGT 
TTAGTCACAG GCACTGATGT CAGCAAGAAA AATGCCACTA CTCAAAACAC TAAGAAGAAA 
AGCTTGTCTG GTAGTGAAGG AAATCACGTG AAACTTTTCC CTAAGTCTAA GCCAGTGGCC 
ATTTCTGTGG CATCTTATAG TCCTATGTCC TCACAGGATC ATTCCAAGAA CCAGCCCTTT 
GATGTGAATA CATCTGGATA CTCCCCACCA CACACAGCAG ATGCCCTCAA GGGTCCAAGG 
ACACATCGGC GCTGCTCAGA GCCCAACATA GAAGACCAGA ACCGCAAGCT GACCTATCTC 
AGGGGAATTT ATTCAAAGAA ACAACATAAA ACCAGCTGTG AAGCTGGTCT CTTGCATGGA 
GAGGAGGATT ATCTCAAACG GCATAAGTCT TTGCAAATGG AGGGGCAGAA GCTCATTAAT 
CAGAGTTTAG TCATGGGGAT TGAGGTGGGC AAGAGTAGTG CCACAAACCA AAACACTGAG 
AAGGTTTTAC CCCCAAGATT AAACCTTTGC CCAAGGACCA GCTATTCCAG CTTATCCTCC 
CCAGGCACTT CCCCATCCGG CTCATCAGTA AGCTCCCAAG ACAGTGCTTT TTCTCAGATT 
TCTGAACACT CTGTGTTTAC ACCCACTGAG ACTTCCTCTC CAATAGATTG CACTTTTCAG 
GCTCAGAGAA AACGGGAAGA CCTTTCTCCT GACTTTAGCA ATGCCAGCCA TGTTTCCGGA 
ATGCCCGGTC CCTCATCAGG GCAGGCTTGC AGCCGCCCAG CCTATACAAA GAAGGACACC 
ATGGAGTGGC ATTCACAAAT GCATTCTGTA ACTCTTCATC CCAGCACATG GTTGAGAAAT 
GGTGTGGCCA GTTTGAAAAA CTGGTCCCTC AAAAAGAAAG CAAAGGCAGC CAGACCAGAG 
GAAGAGAAAA TAGCTTCTCC AAAAGGACCC TTAGAGCCAC CCCCACATGC TTCTGGTGTT 
CCAGAAGCCA ACTCACTGCA AGAGGAACAA AAAGACTTGC CCTTAAGGGC AGCTGAAGGA 
CTGTCCCCTG TGCAGTCAGC CCAAAGGTGT AGTTCTTCTC CCTTCCAGGA CTCAGAGAGA 
CACTGTAGCT CTCCATTCAG CCTGGTGGAG AGCAGACTTA AGCTGTGCAT GAAGTCACAT 
GAGGAAATAG AGCCTGGTAG TCAGAGCTCT TCTGGTTCTC TGCCTTGGGA AAGAGCCTCA 
GCCAGCTCTT GGACTCTAGA GGATGCGACC AGCCCAGACT CAGGGCCTAC AGTGGTCTGC 
GACATTGAGG ACAGGTATTT AACCAAAGAC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718956.1
CDS	195..3770
Translation	MEAMSPQQETLGGQPGRSSSLTGVSRLAGGSCTKKKMKTLAERRRSAPSLILDKALQKRPTTRDSPSASVDTCTFLSSLVCSNRTLLIDGRAELKRGLQRQERHLFLFNDLFVVAKIKYNNNFKIKNKIKLTDMWTASCVDEVGEGNTNAMKSFVLGWPTVNFVATFSSPEQKDKWLSLLQRYINLEKEKDYPKSIPLKIFAKDIGNCAYSKTITVMNSDTANEVINMSLPMLGITGSERDYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDSALLTPGSKDSTTPFNLQEPFLMEQLPREMQCQFILKPSRLAAAQQLSDSGHKTFKRRRSIINWAFWRGSSTHLDNLPSSPTSPMPGQLFGISLPNICENDNLPKPVLDMLFFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGVEVHLDCESIFVIASVLKDFLRNIPGSIFSSDLYDHWVSVMDQGNDEEKINTVQRLLDQLPRANVVLLRYLFGVLHNIEQHSSSNQMTAFNLAVCVAPSILWPPASSSPELENEFTKKVSLLIQFLIENCLRIFGEEITSLFREVSVRCDTRENASDISCFQLNDSSYDSLENELNEDVDAPCSDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLAHSKDEDVQMKRPLESKPVNILVYTKIPLRDHARAPSAMCTPSYLSTAAANAAKSLRRHRRCSEPSIDYLDSKLSYLREFYQKKLRKSSCDAILSQKDEDYLKQNQPLQEEGKTCFKQSLVTGTDVSKKNATTQNTKKKSLSGSEGNHVKLFPKSKPVAISVASYSPMSSQDHSKNQPFDVNTSGYSPPHTADALKGPRTHRRCSEPNIEDQNRKLTYLRGIYSKKQHKTSCEAGLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNTEKVLPPRLNLCPRTSYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQAQRKREDLSPDFSNASHVSGMPGPSSGQACSRPAYTKKDTMEWHSQMHSVTLHPSTWLRNGVASLKNWSLKKKAKAARPEEEKIASPKGPLEPPPHASGVPEANSLQEEQKDLPLRAAEGLSPVQSAQRCSSSPFQDSERHCSSPFSLVESRLKLCMKSHEEIEPGSQSSSGSLPWERASASSWTLEDATSPDSGPTVVCDIEDRYLTKDI

Target ORF information:

RefSeq Version	XM_006718893.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718893.3

ORF Insert Sequence:

ATGGAAGCGA TGTCCCCCCA GCAGGAGACT CTAGGGGGAC AGCCGGGGCG CTCCTCTTCC 
CTGACAGGAG TGTCTCGGCT CGCGGGAGGC AGCTGCACCA AGAAGAAAAT GAAAACACTA 
GCAGAAAGGA GGAGGAGCGC TCCATCTCTT ATCCTGGATA AAGCCCTACA AAAACGGCCT 
ACTACCAGGG ACAGTCCTTC TGCTAGTGTT GACACATGCA CATTTCTGTC ATCATTAGTG 
TGCTCCAATA GGACTCTGCT GATTGATGGC CGGGCAGAAC TCAAAAGAGG CCTCCAGAGG 
CAGGAGCGGC ATCTTTTCCT ATTCAATGAT CTGTTTGTTG TGGCCAAAAT CAAATATAAC 
AATAACTTTA AGATAAAAAA TAAAATTAAA TTAACTGATA TGTGGACAGC AAGCTGTGTG 
GATGAAGTGG GAGAAGGCAA CACCAATGCC ATGAAATCCT TTGTTTTGGG CTGGCCCACA 
GTGAACTTTG TGGCCACTTT CAGTTCTCCA GAACAAAAGG ACAAATGGCT CTCTCTCCTT 
CAGAGATACA TCAATCTAGA GAAAGAAAAG GACTACCCGA AGAGCATTCC CCTCAAAATC 
TTCGCCAAGG ACATTGGGAA TTGTGCCTAC TCTAAAACTA TAACAGTAAT GAATTCAGAT 
ACAGCGAATG AAGTTATCAA CATGTCATTA CCAATGCTAG GGATAACTGG CTCTGAGAGA 
GATTACCAGT TGTGGGTCAA TTCTGGCAAA GAAGAGGCTC CATACCCACT CATTGGGCAT 
GAATATCCAT ATGGAATTAA AATGAGCCAT CTTCGAGACT CTGCACTCCT GACACCGGGA 
TCAAAGGACT CTACCACCCC TTTCAACCTC CAGGAGCCCT TCCTTATGGA ACAGCTCCCC 
CGAGAGATGC AGTGCCAGTT CATCCTGAAG CCCAGCCGCC TGGCTGCAGC CCAGCAACTG 
AGTGATTCAG GTCATAAGAC ATTTAAAAGG AGAAGATCTA TCATAAACTG GGCCTTCTGG 
CGAGGTTCTA GCACTCACCT GGACAACTTG CCCTCATCGC CAACATCACC TATGCCAGGA 
CAGCTCTTTG GAATTTCTCT GCCAAATATT TGTGAGAATG ACAATCTGCC CAAACCTGTC 
TTGGATATGC TTTTCTTTCT TAATCAAAAA GGACCTCTCA CCAAAGGTAT CTTCAGGCAA 
TCAGCCAATG TGAAATCCTG CAGAGAACTA AAAGAGAAAT TGAATTCTGG AGTCGAAGTA 
CACCTAGACT GTGAATCTAT TTTTGTGATA GCATCTGTCT TAAAGGATTT TCTGCGAAAT 
ATTCCAGGAA GTATTTTTTC ATCAGATCTC TATGATCACT GGGTCTCTGT AATGGATCAA 
GGAAATGATG AAGAGAAAAT AAATACTGTT CAAAGGCTAT TAGACCAGCT TCCGAGAGCC 
AATGTTGTTC TCCTAAGGTA TCTTTTTGGG GTGTTACACA ACATTGAGCA ACATTCCTCA 
TCCAATCAGA TGACTGCATT TAATTTAGCT GTGTGTGTCG CTCCAAGTAT TCTTTGGCCT 
CCTGCTTCCT CCAGCCCAGA ACTAGAAAAC GAATTTACAA AAAAGGTTTC CCTGCTTATA 
CAATTTCTGA TTGAGAATTG CCTTAGGATA TTTGGAGAAG AAATCACTTC CCTCTTCAGA 
GAGGTTTCAG TGAGATGTGA CACTAGAGAG AATGCCTCAG ATATTTCTTG CTTTCAACTG 
AATGACTCCT CCTATGACAG CTTGGAAAAT GAGCTAAATG AGGATGTTGA TGCACCATGC 
AGTGACTTGG TAAAGAAACT TGGCCAGGGG AGCAGAAGCA TGGACTCTGT CTTAACCCTC 
AGTGACTATG ATCTTGACCA GCCCGAGGTG GAAGGCCTTT TAACCCTAAG CGACTTTGAC 
TTGGCCCATT CTAAAGATGA AGATGTTCAA ATGAAACGGC CTCTTGAATC CAAGCCGGTG 
AACATTTTAG TGTACACAAA GATCCCACTG CGGGATCATG CCAGGGCCCC ATCTGCCATG 
TGCACACCCA GCTACCTGTC CACAGCTGCA GCAAATGCTG CAAAAAGCCT GAGGCGACAC 
CGGCGTTGCT CAGAGCCCAG CATCGACTAT CTGGATTCAA AGCTTTCCTA CCTCAGGGAG 
TTTTATCAGA AAAAGCTACG CAAGTCCAGC TGTGATGCAA TTCTTTCTCA AAAAGATGAA 
GACTATCTGA AGCAGAATCA ACCCCTCCAG GAGGAAGGAA AGACATGTTT TAAACAGAGT 
TTAGTCACAG GCACTGATGT CAGCAAGAAA AATGCCACTA CTCAAAACAC TAAGAAGAAA 
AGCTTGTCTG GTAGTGAAGG AAATCACGTG AAACTTTTCC CTAAGTCTAA GCCAGTGGCC 
ATTTCTGTGG CATCTTATAG TCCTATGTCC TCACAGGATC ATTCCAAGAA CCAGCCCTTT 
GATGTGAATA CATCTGGATA CTCCCCACCA CACACAGCAG ATGCCCTCAA GGGTCCAAGG 
ACACATCGGC GCTGCTCAGA GCCCAACATA GAAGACCAGA ACCGCAAGCT GACCTATCTC 
AGGGGAATTT ATTCAAAGAA ACAACATAAA ACCAGCTGTG AAGCTGGTCT CTTGCATGGA 
GAGGAGGATT ATCTCAAACG GCATAAGTCT TTGCAAATGG AGGGGCAGAA GCTCATTAAT 
CAGAGTTTAG TCATGGGGAT TGAGGTGGGC AAGAGTAGTG CCACAAACCA AAACACTGAG 
AAGGTTTTAC CCCCAAGATT AAACCTTTGC CCAAGGACCA GCTATTCCAG CTTATCCTCC 
CCAGGCACTT CCCCATCCGG CTCATCAGTA AGCTCCCAAG ACAGTGCTTT TTCTCAGATT 
TCTGAACACT CTGTGTTTAC ACCCACTGAG ACTTCCTCTC CAATAGATTG CACTTTTCAG 
GCTCAGAGAA AACGGGAAGA CCTTTCTCCT GACTTTAGCA ATGCCAGCCA TGTTTCCGGA 
ATGCCCGGTC CCTCATCAGG GCAGGCTTGC AGCCGCCCAG CCTATACAAA GAAGGACACC 
ATGGAGTGGC ATTCACAAAT GCATTCTGTA ACTCTTCATC CCAGCACATG GTTGAGAAAT 
GGTGTGGCCA GTTTGAAAAA CTGGTCCCTC AAAAAGAAAG CAAAGGCAGC CAGACCAGAG 
GAAGAGAAAA TAGCTTCTCC AAAAGGACCC TTAGAGCCAC CCCCACATGC TTCTGGTGTT 
CCAGAAGCCA ACTCACTGCA AGAGGAACAA AAAGACTTGC CCTTAAGGGC AGCTGAAGGA 
CTGTCCCCTG TGCAGTCAGC CCAAAGGTGT AGTTCTTCTC CCTTCCAGGA CTCAGAGAGA 
CACTGTAGCT CTCCATTCAG CCTGGTGGAG AGCAGACTTA AGCTGTGCAT GAAGTCACAT 
GAGGAAATAG AGCCTGGTAG TCAGAGCTCT TCTGGTTCTC TGCCTTGGGA AAGAGCCTCA 
GCCAGCTCTT GGACTCTAGA GGATGCGACC AGCCCAGACT CAGGGCCTAC AGTGGTCTGC 
GACATTGAGG ACAGGTATTT AACCAAAGAC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heritability and genome-wide association analysis of renal sinus fat accumulation in the Framingham Heart Study.
BMC medical genetics12148(2011 Nov)
Foster MC,Yang Q,Hwang SJ,Hoffmann U,Fox CS

Expression analysis of genes located in the minimally deleted regions of 13q14 and 11q22-23 in chronic lymphocytic leukemia-unexpected expression pattern of the RHO GTPase activator ARHGAP20.
Genes, chromosomes & cancer50(7)546-58(2011 Jul)
Herold T,Jurinovic V,Mulaw M,Seiler T,Dufour A,Schneider S,Kakadia PM,Feuring-Buske M,Braess J,Spiekermann K,Mansmann U,Hiddemann W,Buske C,Bohlander SK

RA-RhoGAP, Rap-activated Rho GTPase-activating protein implicated in neurite outgrowth through Rho.
The Journal of biological chemistry280(38)33026-34(2005 Sep)
Yamada T,Sakisaka T,Hisata S,Baba T,Takai Y

Translocation t(X;11)(q13;q23) in B-cell chronic lymphocytic leukemia disrupts two novel genes.
Genes, chromosomes & cancer42(2)128-43(2005 Feb)
Kalla C,Nentwich H,Schlotter M,Mertens D,Wildenberger K,D?hner H,Stilgenbauer S,Lichter P

Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male germ cells.
Genomics84(2)406-18(2004 Aug)
Curry BJ,Su H,Law EG,McLaughlin EA,Nixon B,Aitken RJ

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ARHGAP20 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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