ARHGAP20 cDNA ORF clone, Homo sapiens(human)

The following ARHGAP20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGAP20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu45843 XM_005271628.3
Latest version!
Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu08015 NM_001258416.1
Latest version!
Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu08022 NM_001258418.1
Latest version!
Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu08022 NM_001258417.1
Latest version!
Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu08682 NM_020809.3
Latest version!
Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu12330 NM_001258415.2
Latest version!
Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu08682 XM_006718893.3
Latest version!
Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
View Old Accession Versions >>
OHu12330 NM_001258415.1 Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu45843
    Clone ID Related Accession (Same CDS sequence) XM_005271628.3
    Accession Version XM_005271628.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rho GTPase-activating protein 20 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005271628.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGATCAAG GAAATGATGA AGAGAAAATA AATACTGTTC AAAGGCTATT AGACCAGCTT 
    CCGAGAGCCA ATGTTGTTCT CCTAAGGTAT CTTTTTGGGG TGTTACACAA CATTGAGCAA
    CATTCCTCAT CCAATCAGAT GACTGCATTT AATTTAGCTG TGTGTGTCGC TCCAAGTATT
    CTTTGGCCTC CTGCTTCCTC CAGCCCAGAA CTAGAAAACG AATTTACAAA AAAGGTTTCC
    CTGCTTATAC AATTTCTGAT TGAGAATTGC CTTAGGATAT TTGGAGAAGA AATCACTTCC
    CTCTTCAGAG AGGTTTCAGT GAGATGTGAC ACTAGAGAGA ATGCCTCAGA TATTTCTTGC
    TTTCAACTGA ATGACTCCTC CTATGACAGC TTGGAAAATG AGCTAAATGA GGATGTTGAT
    GCACCATGCA GTGACTTGGT AAAGAAACTT GGCCAGGGGA GCAGAAGCAT GGACTCTGTC
    TTAACCCTCA GTGACTATGA TCTTGACCAG CCCGAGGTGG AAGGCCTTTT AACCCTAAGC
    GACTTTGACT TGGCCCATTC TAAAGATGAA GATGTTCAAA TGAAACGGCC TCTTGAATCC
    AAGCCGGTGA ACATTTTAGT GTACACAAAG ATCCCACTGC GGGATCATGC CAGGGCCCCA
    TCTGCCATGT GCACACCCAG CTACCTGTCC ACAGCTGCAG CAAATGCTGC AAAAAGCCTG
    AGGCGACACC GGCGTTGCTC AGAGCCCAGC ATCGACTATC TGGATTCAAA GCTTTCCTAC
    CTCAGGGAGT TTTATCAGAA AAAGCTACGC AAGTCCAGCT GTGATGCAAT TCTTTCTCAA
    AAAGATGAAG ACTATCTGAA GCAGAATCAA CCCCTCCAGG AGGAAGGAAA GACATGTTTT
    AAACAGAGTT TAGTCACAGG CACTGATGTC AGCAAGAAAA ATGCCACTAC TCAAAACACT
    AAGAAGAAAA GCTTGTCTGG TAGTGAAGGA AATCACGTGA AACTTTTCCC TAAGTCTAAG
    CCAGTGGCCA TTTCTGTGGC ATCTTATAGT CCTATGTCCT CACAGGATCA TTCCAAGAAC
    CAGCCCTTTG ATGTGAATAC ATCTGGATAC TCCCCACCAC ACACAGCAGA TGCCCTCAAG
    GGTCCAAGGA CACATCGGCG CTGCTCAGAG CCCAACATAG AAGACCAGAA CCGCAAGCTG
    ACCTATCTCA GGGGAATTTA TTCAAAGAAA CAACATAAAA CCAGCTGTGA AGCTGGTCTC
    TTGCATGGAG AGGAGGATTA TCTCAAACGG CATAAGTCTT TGCAAATGGA GGGGCAGAAG
    CTCATTAATC AGAGTTTAGT CATGGGGATT GAGGTGGGCA AGAGTAGTGC CACAAACCAA
    AACACTGAGA AGGTTTTACC CCCAAGATTA AACCTTTGCC CAAGGACCAG CTATTCCAGC
    TTATCCTCCC CAGGCACTTC CCCATCCGGC TCATCAGTAA GCTCCCAAGA CAGTGCTTTT
    TCTCAGATTT CTGAACACTC TGTGTTTACA CCCACTGAGA CTTCCTCTCC AATAGATTGC
    ACTTTTCAGG CTCAGAGAAA ACGGGAAGAC CTTTCTCCTG ACTTTAGCAA TGCCAGCCAT
    GTTTCCGGAA TGCCCGGTCC CTCATCAGGG CAGGCTTGCA GCCGCCCAGC CTATACAAAG
    AAGGACACCA TGGAGTGGCA TTCACAAATG CATTCTGTAA CTCTTCATCC CAGCACATGG
    TTGAGAAATG GTGTGGCCAG TTTGAAAAAC TGGTCCCTCA AAAAGAAAGC AAAGGCAGCC
    AGACCAGAGG AAGAGAAAAT AGCTTCTCCA AAAGGACCCT TAGAGCCACC CCCACATGCT
    TCTGGTGTTC CAGAAGCCAA CTCACTGCAA GAGGAACAAA AAGACTTGCC CTTAAGGGCA
    GCTGAAGGAC TGTCCCCTGT GCAGTCAGCC CAAAGGTGTA GTTCTTCTCC CTTCCAGGAC
    TCAGAGAGAC ACTGTAGCTC TCCATTCAGC CTGGTGGAGA GCAGACTTAA GCTGTGCATG
    AAGTCACATG AGGAAATAGA GCCTGGTAGT CAGAGCTCTT CTGGTTCTCT GCCTTGGGAA
    AGAGCCTCAG CCAGCTCTTG GACTCTAGAG GATGCGACCA GCCCAGACTC AGGGCCTACA
    GTGGTCTGCG ACATTGAGGA CAGGTATTTA ACCAAAGACA TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005271685.1
    CDS331..2535
    Translation

    Target ORF information:

    RefSeq Version XM_005271628.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005271628.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGATCAAG GAAATGATGA AGAGAAAATA AATACTGTTC AAAGGCTATT AGACCAGCTT 
    CCGAGAGCCA ATGTTGTTCT CCTAAGGTAT CTTTTTGGGG TGTTACACAA CATTGAGCAA
    CATTCCTCAT CCAATCAGAT GACTGCATTT AATTTAGCTG TGTGTGTCGC TCCAAGTATT
    CTTTGGCCTC CTGCTTCCTC CAGCCCAGAA CTAGAAAACG AATTTACAAA AAAGGTTTCC
    CTGCTTATAC AATTTCTGAT TGAGAATTGC CTTAGGATAT TTGGAGAAGA AATCACTTCC
    CTCTTCAGAG AGGTTTCAGT GAGATGTGAC ACTAGAGAGA ATGCCTCAGA TATTTCTTGC
    TTTCAACTGA ATGACTCCTC CTATGACAGC TTGGAAAATG AGCTAAATGA GGATGTTGAT
    GCACCATGCA GTGACTTGGT AAAGAAACTT GGCCAGGGGA GCAGAAGCAT GGACTCTGTC
    TTAACCCTCA GTGACTATGA TCTTGACCAG CCCGAGGTGG AAGGCCTTTT AACCCTAAGC
    GACTTTGACT TGGCCCATTC TAAAGATGAA GATGTTCAAA TGAAACGGCC TCTTGAATCC
    AAGCCGGTGA ACATTTTAGT GTACACAAAG ATCCCACTGC GGGATCATGC CAGGGCCCCA
    TCTGCCATGT GCACACCCAG CTACCTGTCC ACAGCTGCAG CAAATGCTGC AAAAAGCCTG
    AGGCGACACC GGCGTTGCTC AGAGCCCAGC ATCGACTATC TGGATTCAAA GCTTTCCTAC
    CTCAGGGAGT TTTATCAGAA AAAGCTACGC AAGTCCAGCT GTGATGCAAT TCTTTCTCAA
    AAAGATGAAG ACTATCTGAA GCAGAATCAA CCCCTCCAGG AGGAAGGAAA GACATGTTTT
    AAACAGAGTT TAGTCACAGG CACTGATGTC AGCAAGAAAA ATGCCACTAC TCAAAACACT
    AAGAAGAAAA GCTTGTCTGG TAGTGAAGGA AATCACGTGA AACTTTTCCC TAAGTCTAAG
    CCAGTGGCCA TTTCTGTGGC ATCTTATAGT CCTATGTCCT CACAGGATCA TTCCAAGAAC
    CAGCCCTTTG ATGTGAATAC ATCTGGATAC TCCCCACCAC ACACAGCAGA TGCCCTCAAG
    GGTCCAAGGA CACATCGGCG CTGCTCAGAG CCCAACATAG AAGACCAGAA CCGCAAGCTG
    ACCTATCTCA GGGGAATTTA TTCAAAGAAA CAACATAAAA CCAGCTGTGA AGCTGGTCTC
    TTGCATGGAG AGGAGGATTA TCTCAAACGG CATAAGTCTT TGCAAATGGA GGGGCAGAAG
    CTCATTAATC AGAGTTTAGT CATGGGGATT GAGGTGGGCA AGAGTAGTGC CACAAACCAA
    AACACTGAGA AGGTTTTACC CCCAAGATTA AACCTTTGCC CAAGGACCAG CTATTCCAGC
    TTATCCTCCC CAGGCACTTC CCCATCCGGC TCATCAGTAA GCTCCCAAGA CAGTGCTTTT
    TCTCAGATTT CTGAACACTC TGTGTTTACA CCCACTGAGA CTTCCTCTCC AATAGATTGC
    ACTTTTCAGG CTCAGAGAAA ACGGGAAGAC CTTTCTCCTG ACTTTAGCAA TGCCAGCCAT
    GTTTCCGGAA TGCCCGGTCC CTCATCAGGG CAGGCTTGCA GCCGCCCAGC CTATACAAAG
    AAGGACACCA TGGAGTGGCA TTCACAAATG CATTCTGTAA CTCTTCATCC CAGCACATGG
    TTGAGAAATG GTGTGGCCAG TTTGAAAAAC TGGTCCCTCA AAAAGAAAGC AAAGGCAGCC
    AGACCAGAGG AAGAGAAAAT AGCTTCTCCA AAAGGACCCT TAGAGCCACC CCCACATGCT
    TCTGGTGTTC CAGAAGCCAA CTCACTGCAA GAGGAACAAA AAGACTTGCC CTTAAGGGCA
    GCTGAAGGAC TGTCCCCTGT GCAGTCAGCC CAAAGGTGTA GTTCTTCTCC CTTCCAGGAC
    TCAGAGAGAC ACTGTAGCTC TCCATTCAGC CTGGTGGAGA GCAGACTTAA GCTGTGCATG
    AAGTCACATG AGGAAATAGA GCCTGGTAGT CAGAGCTCTT CTGGTTCTCT GCCTTGGGAA
    AGAGCCTCAG CCAGCTCTTG GACTCTAGAG GATGCGACCA GCCCAGACTC AGGGCCTACA
    GTGGTCTGCG ACATTGAGGA CAGGTATTTA ACCAAAGACA TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08015
    Clone ID Related Accession (Same CDS sequence) NM_001258416.1
    Accession Version NM_001258416.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-24
    Organism Homo sapiens(human)
    Product rho GTPase-activating protein 20 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY496267.1 and AP001883.6. Transcript Variant: This variant (3) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY496267.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACATTTT GGATTATAAT AAATAAGAAA ATGAAAACAC TAGCAGAAAG GAGGAGGAGC 
    GCTCCATCTC TTATCCTGGA TAAAGCCCTA CAAAAACGGC CTACTACCAG GGACAGTCCT
    TCTGCTAGTG TTGACACATG CACATTTCTG TCATCATTAG TGTGCTCCAA TAGGACTCTG
    CTGATTGATG GCCGGGCAGA ACTCAAAAGA GGCCTCCAGA GGCAGGAGCG GCATCTTTTC
    CTATTCAATG ATCTGTTTGT TGTGGCCAAA ATCAAATATA ACAATAACTT TAAGATAAAA
    AATAAAATTA AATTAACTGA TATGTGGACA GCAAGCTGTG TGGATGAAGT GGGAGAAGGC
    AACACCAATG CCATGAAATC CTTTGTTTTG GGCTGGCCCA CAGTGAACTT TGTGGCCACT
    TTCAGTTCTC CAGAACAAAA GGACAAATGG CTCTCTCTCC TTCAGAGATA CATCAATCTA
    GAGAAAGAAA AGGACTACCC GAAGAGCATT CCCCTCAAAA TCTTCGCCAA GGACATTGGG
    AATTGTGCCT ACTCTAAAAC TATAACAGTA ATGAATTCAG ATACAGCGAA TGAAGTTATC
    AACATGTCAT TACCAATGCT AGGGATAACT GGCTCTGAGA GAGATTACCA GTTGTGGGTC
    AATTCTGGCA AAGAAGAGGC TCCATACCCA CTCATTGGGC ATGAATATCC ATATGGAATT
    AAAATGAGCC ATCTTCGAGA CTCTGCACTC CTGACACCGG GATCAAAGGA CTCTACCACC
    CCTTTCAACC TCCAGGAGCC CTTCCTTATG GAACAGCTCC CCCGAGAGAT GCAGTGCCAG
    TTCATCCTGA AGCCCAGCCG CCTGGCTGCA GCCCAGCAAC TGAGTGATTC AGGTCATAAG
    ACATTTAAAA GGAGAAGATC TATCATAAAC TGGGCCTTCT GGCGAGGTTC TAGCACTCAC
    CTGGACAACT TGCCCTCATC GCCAACATCA CCTATGCCAG GACAGCTCTT TGGAATTTCT
    CTGCCAAATA TTTGTGAGAA TGACAATCTG CCCAAACCTG TCTTGGATAT GCTTTTCTTT
    CTTAATCAAA AAGGACCTCT CACCAAAGGT ATCTTCAGGC AATCAGCCAA TGTGAAATCC
    TGCAGAGAAC TAAAAGAGAA ATTGAATTCT GGAGTCGAAG TACACCTAGA CTGTGAATCT
    ATTTTTGTGA TAGCATCTGT CTTAAAGGAT TTTCTGCGAA ATATTCCAGG AAGTATTTTT
    TCATCAGATC TCTATGATCA CTGGGTCTCT GTAATGGATC AAGGAAATGA TGAAGAGAAA
    ATAAATACTG TTCAAAGGCT ATTAGACCAG CTTCCGAGAG CCAATGTTGT TCTCCTAAGG
    TATCTTTTTG GGGTGTTACA CAACATTGAG CAACATTCCT CATCCAATCA GATGACTGCA
    TTTAATTTAG CTGTGTGTGT CGCTCCAAGT ATTCTTTGGC CTCCTGCTTC CTCCAGCCCA
    GAACTAGAAA ACGAATTTAC AAAAAAGGTT TCCCTGCTTA TACAATTTCT GATTGAGAAT
    TGCCTTAGGA TATTTGGAGA AGAAATCACT TCCCTCTTCA GAGAGGTTTC AGTGAGATGT
    GACACTAGAG AGAATGCCTC AGATATTTCT TGCTTTCAAC TGAATGACTC CTCCTATGAC
    AGCTTGGAAA ATGAGCTAAA TGAGGATGTT GATGCACCAT GCAGTGACTT GGTAAAGAAA
    CTTGGCCAGG GGAGCAGAAG CATGGACTCT GTCTTAACCC TCAGTGACTA TGATCTTGAC
    CAGCCCGAGG TGGAAGGCCT TTTAACCCTA AGCGACTTTG ACTTGGCCCA TTCTAAAGAT
    GAAGATGTTC AAATGAAACG GCCTCTTGAA TCCAAGCCGG TGAACATTTT AGTGTACACA
    AAGATCCCAC TGCGGGATCA TGCCAGGGCC CCATCTGCCA TGTGCACACC CAGCTACCTG
    TCCACAGCTG CAGCAAATGC TGCAAAAAGC CTGAGGCGAC ACCGGCGTTG CTCAGAGCCC
    AGCATCGACT ATCTGGATTC AAAGCTTTCC TACCTCAGGG AGTTTTATCA GAAAAAGCTA
    CGCAAGTCCA GCTGTGATGC AATTCTTTCT CAAAAAGATG AAGACTATCT GAAGCAGAAT
    CAACCCCTCC AGGAGGAAGG AAAGACATGT TTTAAACAGA GTTTAGTCAC AGGCACTGAT
    GTCAGCAAGA AAAATGCCAC TACTCAAAAC ACTAAGAAGA AAAGCTTGTC TGGTAGTGAA
    GGAAATCACG TGAAACTTTT CCCTAAGTCT AAGCCAGTGG CCATTTCTGT GGCATCTTAT
    AGTCCTATGT CCTCACAGGA TCATTCCAAG AACCAGCCCT TTGATGTGAA TACATCTGGA
    TACTCCCCAC CACACACAGC AGATGCCCTC AAGGGTCCAA GGACACATCG GCGCTGCTCA
    GAGCCCAACA TAGAAGACCA GAACCGCAAG CTGACCTATC TCAGGGGAAT TTATTCAAAG
    AAACAACATA AAACCAGCTG TGAAGCTGGT CTCTTGCATG GAGAGGAGGA TTATCTCAAA
    CGGCATAAGT CTTTGCAAAT GGAGGGGCAG AAGCTCATTA ATCAGAGTTT AGTCATGGGG
    ATTGAGGTGG GCAAGAGTAG TGCCACAAAC CAAAACACTG AGAAGGTTTT ACCCCCAAGA
    TTAAACCTTT GCCCAAGGAC CAGCTATTCC AGCTTATCCT CCCCAGGCAC TTCCCCATCC
    GGCTCATCAG TAAGCTCCCA AGACAGTGCT TTTTCTCAGA TTTCTGAACA CTCTGTGTTT
    ACACCCACTG AGACTTCCTC TCCAATAGAT TGCACTTTTC AGGCTCAGAG AAAACGGGAA
    GACCTTTCTC CTGACTTTAG CAATGCCAGC CATGTTTCCG GAATGCCCGG TCCCTCATCA
    GGGCAGGCTT GCAGCCGCCC AGCCTATACA AAGAAGGACA CCATGGAGTG GCATTCACAA
    ATGCATTCTG TAACTCTTCA TCCCAGCACA TGGTTGAGAA ATGGTGTGGC CAGTTTGAAA
    AACTGGTCCC TCAAAAAGAA AGCAAAGGCA GCCAGACCAG AGGAAGAGAA AATAGCTTCT
    CCAAAAGGAC CCTTAGAGCC ACCCCCACAT GCTTCTGGTG TTCCAGAAGC CAACTCACTG
    CAAGAGGAAC AAAAAGACTT GCCCTTAAGG GCAGCTGAAG GACTGTCCCC TGTGCAGTCA
    GCCCAAAGGT GTAGTTCTTC TCCCTTCCAG GACTCAGAGA GACACTGTAG CTCTCCATTC
    AGCCTGGTGG AGAGCAGACT TAAGCTGTGC ATGAAGTCAC ATGAGGAAAT AGAGCCTGGT
    AGTCAGAGCT CTTCTGGTTC TCTGCCTTGG GAAAGAGCCT CAGCCAGCTC TTGGACTCTA
    GAGGATGCGA CCAGCCCAGA CTCAGGGCCT ACAGTGGTCT GCGACATTGA GGACAGGTAT
    TTAACCAAAG ACATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245345.1
    CDS253..3750
    Translation

    Target ORF information:

    RefSeq Version NM_001258416.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258416.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACATTTT GGATTATAAT AAATAAGAAA ATGAAAACAC TAGCAGAAAG GAGGAGGAGC 
    GCTCCATCTC TTATCCTGGA TAAAGCCCTA CAAAAACGGC CTACTACCAG GGACAGTCCT
    TCTGCTAGTG TTGACACATG CACATTTCTG TCATCATTAG TGTGCTCCAA TAGGACTCTG
    CTGATTGATG GCCGGGCAGA ACTCAAAAGA GGCCTCCAGA GGCAGGAGCG GCATCTTTTC
    CTATTCAATG ATCTGTTTGT TGTGGCCAAA ATCAAATATA ACAATAACTT TAAGATAAAA
    AATAAAATTA AATTAACTGA TATGTGGACA GCAAGCTGTG TGGATGAAGT GGGAGAAGGC
    AACACCAATG CCATGAAATC CTTTGTTTTG GGCTGGCCCA CAGTGAACTT TGTGGCCACT
    TTCAGTTCTC CAGAACAAAA GGACAAATGG CTCTCTCTCC TTCAGAGATA CATCAATCTA
    GAGAAAGAAA AGGACTACCC GAAGAGCATT CCCCTCAAAA TCTTCGCCAA GGACATTGGG
    AATTGTGCCT ACTCTAAAAC TATAACAGTA ATGAATTCAG ATACAGCGAA TGAAGTTATC
    AACATGTCAT TACCAATGCT AGGGATAACT GGCTCTGAGA GAGATTACCA GTTGTGGGTC
    AATTCTGGCA AAGAAGAGGC TCCATACCCA CTCATTGGGC ATGAATATCC ATATGGAATT
    AAAATGAGCC ATCTTCGAGA CTCTGCACTC CTGACACCGG GATCAAAGGA CTCTACCACC
    CCTTTCAACC TCCAGGAGCC CTTCCTTATG GAACAGCTCC CCCGAGAGAT GCAGTGCCAG
    TTCATCCTGA AGCCCAGCCG CCTGGCTGCA GCCCAGCAAC TGAGTGATTC AGGTCATAAG
    ACATTTAAAA GGAGAAGATC TATCATAAAC TGGGCCTTCT GGCGAGGTTC TAGCACTCAC
    CTGGACAACT TGCCCTCATC GCCAACATCA CCTATGCCAG GACAGCTCTT TGGAATTTCT
    CTGCCAAATA TTTGTGAGAA TGACAATCTG CCCAAACCTG TCTTGGATAT GCTTTTCTTT
    CTTAATCAAA AAGGACCTCT CACCAAAGGT ATCTTCAGGC AATCAGCCAA TGTGAAATCC
    TGCAGAGAAC TAAAAGAGAA ATTGAATTCT GGAGTCGAAG TACACCTAGA CTGTGAATCT
    ATTTTTGTGA TAGCATCTGT CTTAAAGGAT TTTCTGCGAA ATATTCCAGG AAGTATTTTT
    TCATCAGATC TCTATGATCA CTGGGTCTCT GTAATGGATC AAGGAAATGA TGAAGAGAAA
    ATAAATACTG TTCAAAGGCT ATTAGACCAG CTTCCGAGAG CCAATGTTGT TCTCCTAAGG
    TATCTTTTTG GGGTGTTACA CAACATTGAG CAACATTCCT CATCCAATCA GATGACTGCA
    TTTAATTTAG CTGTGTGTGT CGCTCCAAGT ATTCTTTGGC CTCCTGCTTC CTCCAGCCCA
    GAACTAGAAA ACGAATTTAC AAAAAAGGTT TCCCTGCTTA TACAATTTCT GATTGAGAAT
    TGCCTTAGGA TATTTGGAGA AGAAATCACT TCCCTCTTCA GAGAGGTTTC AGTGAGATGT
    GACACTAGAG AGAATGCCTC AGATATTTCT TGCTTTCAAC TGAATGACTC CTCCTATGAC
    AGCTTGGAAA ATGAGCTAAA TGAGGATGTT GATGCACCAT GCAGTGACTT GGTAAAGAAA
    CTTGGCCAGG GGAGCAGAAG CATGGACTCT GTCTTAACCC TCAGTGACTA TGATCTTGAC
    CAGCCCGAGG TGGAAGGCCT TTTAACCCTA AGCGACTTTG ACTTGGCCCA TTCTAAAGAT
    GAAGATGTTC AAATGAAACG GCCTCTTGAA TCCAAGCCGG TGAACATTTT AGTGTACACA
    AAGATCCCAC TGCGGGATCA TGCCAGGGCC CCATCTGCCA TGTGCACACC CAGCTACCTG
    TCCACAGCTG CAGCAAATGC TGCAAAAAGC CTGAGGCGAC ACCGGCGTTG CTCAGAGCCC
    AGCATCGACT ATCTGGATTC AAAGCTTTCC TACCTCAGGG AGTTTTATCA GAAAAAGCTA
    CGCAAGTCCA GCTGTGATGC AATTCTTTCT CAAAAAGATG AAGACTATCT GAAGCAGAAT
    CAACCCCTCC AGGAGGAAGG AAAGACATGT TTTAAACAGA GTTTAGTCAC AGGCACTGAT
    GTCAGCAAGA AAAATGCCAC TACTCAAAAC ACTAAGAAGA AAAGCTTGTC TGGTAGTGAA
    GGAAATCACG TGAAACTTTT CCCTAAGTCT AAGCCAGTGG CCATTTCTGT GGCATCTTAT
    AGTCCTATGT CCTCACAGGA TCATTCCAAG AACCAGCCCT TTGATGTGAA TACATCTGGA
    TACTCCCCAC CACACACAGC AGATGCCCTC AAGGGTCCAA GGACACATCG GCGCTGCTCA
    GAGCCCAACA TAGAAGACCA GAACCGCAAG CTGACCTATC TCAGGGGAAT TTATTCAAAG
    AAACAACATA AAACCAGCTG TGAAGCTGGT CTCTTGCATG GAGAGGAGGA TTATCTCAAA
    CGGCATAAGT CTTTGCAAAT GGAGGGGCAG AAGCTCATTA ATCAGAGTTT AGTCATGGGG
    ATTGAGGTGG GCAAGAGTAG TGCCACAAAC CAAAACACTG AGAAGGTTTT ACCCCCAAGA
    TTAAACCTTT GCCCAAGGAC CAGCTATTCC AGCTTATCCT CCCCAGGCAC TTCCCCATCC
    GGCTCATCAG TAAGCTCCCA AGACAGTGCT TTTTCTCAGA TTTCTGAACA CTCTGTGTTT
    ACACCCACTG AGACTTCCTC TCCAATAGAT TGCACTTTTC AGGCTCAGAG AAAACGGGAA
    GACCTTTCTC CTGACTTTAG CAATGCCAGC CATGTTTCCG GAATGCCCGG TCCCTCATCA
    GGGCAGGCTT GCAGCCGCCC AGCCTATACA AAGAAGGACA CCATGGAGTG GCATTCACAA
    ATGCATTCTG TAACTCTTCA TCCCAGCACA TGGTTGAGAA ATGGTGTGGC CAGTTTGAAA
    AACTGGTCCC TCAAAAAGAA AGCAAAGGCA GCCAGACCAG AGGAAGAGAA AATAGCTTCT
    CCAAAAGGAC CCTTAGAGCC ACCCCCACAT GCTTCTGGTG TTCCAGAAGC CAACTCACTG
    CAAGAGGAAC AAAAAGACTT GCCCTTAAGG GCAGCTGAAG GACTGTCCCC TGTGCAGTCA
    GCCCAAAGGT GTAGTTCTTC TCCCTTCCAG GACTCAGAGA GACACTGTAG CTCTCCATTC
    AGCCTGGTGG AGAGCAGACT TAAGCTGTGC ATGAAGTCAC ATGAGGAAAT AGAGCCTGGT
    AGTCAGAGCT CTTCTGGTTC TCTGCCTTGG GAAAGAGCCT CAGCCAGCTC TTGGACTCTA
    GAGGATGCGA CCAGCCCAGA CTCAGGGCCT ACAGTGGTCT GCGACATTGA GGACAGGTAT
    TTAACCAAAG ACATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08022
    Clone ID Related Accession (Same CDS sequence) NM_001258418.1 , NM_001258417.1
    Accession Version NM_001258418.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product rho GTPase-activating protein 20 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY496265.1 and AP001883.6. Transcript Variant: This variant (5) differs in the 5' UTR and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 4 and 5 encode the same isoform (4), which has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY496265.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAAACAC TAGCAGAAAG GAGGAGGAGC GCTCCATCTC TTATCCTGGA TAAAGCCCTA 
    CAAAAACGGC CTACTACCAG GGACAGTCCT TCTGCTAGTG TTGACACATG CACATTTCTG
    TCATCATTAG TGTGCTCCAA TAGGACTCTG CTGATTGATG GCCGGGCAGA ACTCAAAAGA
    GGCCTCCAGA GGCAGGAGCG GCATCTTTTC CTATTCAATG ATCTGTTTGT TGTGGCCAAA
    ATCAAATATA ACAATAACTT TAAGATAAAA AATAAAATTA AATTAACTGA TATGTGGACA
    GCAAGCTGTG TGGATGAAGT GGGAGAAGGC AACACCAATG CCATGAAATC CTTTGTTTTG
    GGCTGGCCCA CAGTGAACTT TGTGGCCACT TTCAGTTCTC CAGAACAAAA GGACAAATGG
    CTCTCTCTCC TTCAGAGATA CATCAATCTA GAGAAAGAAA AGGACTACCC GAAGAGCATT
    CCCCTCAAAA TCTTCGCCAA GGACATTGGG AATTGTGCCT ACTCTAAAAC TATAACAGTA
    ATGAATTCAG ATACAGCGAA TGAAGTTATC AACATGTCAT TACCAATGCT AGGGATAACT
    GGCTCTGAGA GAGATTACCA GTTGTGGGTC AATTCTGGCA AAGAAGAGGC TCCATACCCA
    CTCATTGGGC ATGAATATCC ATATGGAATT AAAATGAGCC ATCTTCGAGA CTCTGCACTC
    CTGACACCGG GATCAAAGGA CTCTACCACC CCTTTCAACC TCCAGGAGCC CTTCCTTATG
    GAACAGCTCC CCCGAGAGAT GCAGTGCCAG TTCATCCTGA AGCCCAGCCG CCTGGCTGCA
    GCCCAGCAAC TGAGTGATTC AGGTCATAAG ACATTTAAAA GGAGAAGATC TATCATAAAC
    TGGGCCTTCT GGCGAGGTTC TAGCACTCAC CTGGACAACT TGCCCTCATC GCCAACATCA
    CCTATGCCAG GACAGCTCTT TGGAATTTCT CTGCCAAATA TTTGTGAGAA TGACAATCTG
    CCCAAACCTG TCTTGGATAT GCTTTTCTTT CTTAATCAAA AAGGACCTCT CACCAAAGGT
    ATCTTCAGGC AATCAGCCAA TGTGAAATCC TGCAGAGAAC TAAAAGAGAA ATTGAATTCT
    GGAGTCGAAG TACACCTAGA CTGTGAATCT ATTTTTGTGA TAGCATCTGT CTTAAAGGAT
    TTTCTGCGAA ATATTCCAGG AAGTATTTTT TCATCAGATC TCTATGATCA CTGGGTCTCT
    GTAATGGATC AAGGAAATGA TGAAGAGAAA ATAAATACTG TTCAAAGGCT ATTAGACCAG
    CTTCCGAGAG CCAATGTTGT TCTCCTAAGG TATCTTTTTG GGGTGTTACA CAACATTGAG
    CAACATTCCT CATCCAATCA GATGACTGCA TTTAATTTAG CTGTGTGTGT CGCTCCAAGT
    ATTCTTTGGC CTCCTGCTTC CTCCAGCCCA GAACTAGAAA ACGAATTTAC AAAAAAGGTT
    TCCCTGCTTA TACAATTTCT GATTGAGAAT TGCCTTAGGA TATTTGGAGA AGAAATCACT
    TCCCTCTTCA GAGAGGTTTC AGTGAGATGT GACACTAGAG AGAATGCCTC AGATATTTCT
    TGCTTTCAAC TGAATGACTC CTCCTATGAC AGCTTGGAAA ATGAGCTAAA TGAGGATGTT
    GATGCACCAT GCAGTGACTT GGTAAAGAAA CTTGGCCAGG GGAGCAGAAG CATGGACTCT
    GTCTTAACCC TCAGTGACTA TGATCTTGAC CAGCCCGAGG TGGAAGGCCT TTTAACCCTA
    AGCGACTTTG ACTTGGCCCA TTCTAAAGAT GAAGATGTTC AAATGAAACG GCCTCTTGAA
    TCCAAGCCGG TGAACATTTT AGTGTACACA AAGATCCCAC TGCGGGATCA TGCCAGGGCC
    CCATCTGCCA TGTGCACACC CAGCTACCTG TCCACAGCTG CAGCAAATGC TGCAAAAAGC
    CTGAGGCGAC ACCGGCGTTG CTCAGAGCCC AGCATCGACT ATCTGGATTC AAAGCTTTCC
    TACCTCAGGG AGTTTTATCA GAAAAAGCTA CGCAAGTCCA GCTGTGATGC AATTCTTTCT
    CAAAAAGATG AAGACTATCT GAAGCAGAAT CAACCCCTCC AGGAGGAAGG AAAGACATGT
    TTTAAACAGA GTTTAGTCAC AGGCACTGAT GTCAGCAAGA AAAATGCCAC TACTCAAAAC
    ACTAAGAAGA AAAGCTTGTC TGGTAGTGAA GGAAATCACG TGAAACTTTT CCCTAAGTCT
    AAGCCAGTGG CCATTTCTGT GGCATCTTAT AGTCCTATGT CCTCACAGGA TCATTCCAAG
    AACCAGCCCT TTGATGTGAA TACATCTGGA TACTCCCCAC CACACACAGC AGATGCCCTC
    AAGGGTCCAA GGACACATCG GCGCTGCTCA GAGCCCAACA TAGAAGACCA GAACCGCAAG
    CTGACCTATC TCAGGGGAAT TTATTCAAAG AAACAACATA AAACCAGCTG TGAAGCTGGT
    CTCTTGCATG GAGAGGAGGA TTATCTCAAA CGGCATAAGT CTTTGCAAAT GGAGGGGCAG
    AAGCTCATTA ATCAGAGTTT AGTCATGGGG ATTGAGGTGG GCAAGAGTAG TGCCACAAAC
    CAAAACACTG AGAAGGTTTT ACCCCCAAGA TTAAACCTTT GCCCAAGGAC CAGCTATTCC
    AGCTTATCCT CCCCAGGCAC TTCCCCATCC GGCTCATCAG TAAGCTCCCA AGACAGTGCT
    TTTTCTCAGA TTTCTGAACA CTCTGTGTTT ACACCCACTG AGACTTCCTC TCCAATAGAT
    TGCACTTTTC AGGCTCAGAG AAAACGGGAA GACCTTTCTC CTGACTTTAG CAATGCCAGC
    CATGTTTCCG GAATGCCCGG TCCCTCATCA GGGCAGGCTT GCAGCCGCCC AGCCTATACA
    AAGAAGGACA CCATGGAGTG GCATTCACAA ATGCATTCTG TAACTCTTCA TCCCAGCACA
    TGGTTGAGAA ATGGTGTGGC CAGTTTGAAA AACTGGTCCC TCAAAAAGAA AGCAAAGGCA
    GCCAGACCAG AGGAAGAGAA AATAGCTTCT CCAAAAGGAC CCTTAGAGCC ACCCCCACAT
    GCTTCTGGTG TTCCAGAAGC CAACTCACTG CAAGAGGAAC AAAAAGACTT GCCCTTAAGG
    GCAGCTGAAG GACTGTCCCC TGTGCAGTCA GCCCAAAGGT GTAGTTCTTC TCCCTTCCAG
    GACTCAGAGA GACACTGTAG CTCTCCATTC AGCCTGGTGG AGAGCAGACT TAAGCTGTGC
    ATGAAGTCAC ATGAGGAAAT AGAGCCTGGT AGTCAGAGCT CTTCTGGTTC TCTGCCTTGG
    GAAAGAGCCT CAGCCAGCTC TTGGACTCTA GAGGATGCGA CCAGCCCAGA CTCAGGGCCT
    ACAGTGGTCT GCGACATTGA GGACAGGTAT TTAACCAAAG ACATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245347.1
    CDS215..3682
    Translation

    Target ORF information:

    RefSeq Version NM_001258418.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258418.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAAACAC TAGCAGAAAG GAGGAGGAGC GCTCCATCTC TTATCCTGGA TAAAGCCCTA 
    CAAAAACGGC CTACTACCAG GGACAGTCCT TCTGCTAGTG TTGACACATG CACATTTCTG
    TCATCATTAG TGTGCTCCAA TAGGACTCTG CTGATTGATG GCCGGGCAGA ACTCAAAAGA
    GGCCTCCAGA GGCAGGAGCG GCATCTTTTC CTATTCAATG ATCTGTTTGT TGTGGCCAAA
    ATCAAATATA ACAATAACTT TAAGATAAAA AATAAAATTA AATTAACTGA TATGTGGACA
    GCAAGCTGTG TGGATGAAGT GGGAGAAGGC AACACCAATG CCATGAAATC CTTTGTTTTG
    GGCTGGCCCA CAGTGAACTT TGTGGCCACT TTCAGTTCTC CAGAACAAAA GGACAAATGG
    CTCTCTCTCC TTCAGAGATA CATCAATCTA GAGAAAGAAA AGGACTACCC GAAGAGCATT
    CCCCTCAAAA TCTTCGCCAA GGACATTGGG AATTGTGCCT ACTCTAAAAC TATAACAGTA
    ATGAATTCAG ATACAGCGAA TGAAGTTATC AACATGTCAT TACCAATGCT AGGGATAACT
    GGCTCTGAGA GAGATTACCA GTTGTGGGTC AATTCTGGCA AAGAAGAGGC TCCATACCCA
    CTCATTGGGC ATGAATATCC ATATGGAATT AAAATGAGCC ATCTTCGAGA CTCTGCACTC
    CTGACACCGG GATCAAAGGA CTCTACCACC CCTTTCAACC TCCAGGAGCC CTTCCTTATG
    GAACAGCTCC CCCGAGAGAT GCAGTGCCAG TTCATCCTGA AGCCCAGCCG CCTGGCTGCA
    GCCCAGCAAC TGAGTGATTC AGGTCATAAG ACATTTAAAA GGAGAAGATC TATCATAAAC
    TGGGCCTTCT GGCGAGGTTC TAGCACTCAC CTGGACAACT TGCCCTCATC GCCAACATCA
    CCTATGCCAG GACAGCTCTT TGGAATTTCT CTGCCAAATA TTTGTGAGAA TGACAATCTG
    CCCAAACCTG TCTTGGATAT GCTTTTCTTT CTTAATCAAA AAGGACCTCT CACCAAAGGT
    ATCTTCAGGC AATCAGCCAA TGTGAAATCC TGCAGAGAAC TAAAAGAGAA ATTGAATTCT
    GGAGTCGAAG TACACCTAGA CTGTGAATCT ATTTTTGTGA TAGCATCTGT CTTAAAGGAT
    TTTCTGCGAA ATATTCCAGG AAGTATTTTT TCATCAGATC TCTATGATCA CTGGGTCTCT
    GTAATGGATC AAGGAAATGA TGAAGAGAAA ATAAATACTG TTCAAAGGCT ATTAGACCAG
    CTTCCGAGAG CCAATGTTGT TCTCCTAAGG TATCTTTTTG GGGTGTTACA CAACATTGAG
    CAACATTCCT CATCCAATCA GATGACTGCA TTTAATTTAG CTGTGTGTGT CGCTCCAAGT
    ATTCTTTGGC CTCCTGCTTC CTCCAGCCCA GAACTAGAAA ACGAATTTAC AAAAAAGGTT
    TCCCTGCTTA TACAATTTCT GATTGAGAAT TGCCTTAGGA TATTTGGAGA AGAAATCACT
    TCCCTCTTCA GAGAGGTTTC AGTGAGATGT GACACTAGAG AGAATGCCTC AGATATTTCT
    TGCTTTCAAC TGAATGACTC CTCCTATGAC AGCTTGGAAA ATGAGCTAAA TGAGGATGTT
    GATGCACCAT GCAGTGACTT GGTAAAGAAA CTTGGCCAGG GGAGCAGAAG CATGGACTCT
    GTCTTAACCC TCAGTGACTA TGATCTTGAC CAGCCCGAGG TGGAAGGCCT TTTAACCCTA
    AGCGACTTTG ACTTGGCCCA TTCTAAAGAT GAAGATGTTC AAATGAAACG GCCTCTTGAA
    TCCAAGCCGG TGAACATTTT AGTGTACACA AAGATCCCAC TGCGGGATCA TGCCAGGGCC
    CCATCTGCCA TGTGCACACC CAGCTACCTG TCCACAGCTG CAGCAAATGC TGCAAAAAGC
    CTGAGGCGAC ACCGGCGTTG CTCAGAGCCC AGCATCGACT ATCTGGATTC AAAGCTTTCC
    TACCTCAGGG AGTTTTATCA GAAAAAGCTA CGCAAGTCCA GCTGTGATGC AATTCTTTCT
    CAAAAAGATG AAGACTATCT GAAGCAGAAT CAACCCCTCC AGGAGGAAGG AAAGACATGT
    TTTAAACAGA GTTTAGTCAC AGGCACTGAT GTCAGCAAGA AAAATGCCAC TACTCAAAAC
    ACTAAGAAGA AAAGCTTGTC TGGTAGTGAA GGAAATCACG TGAAACTTTT CCCTAAGTCT
    AAGCCAGTGG CCATTTCTGT GGCATCTTAT AGTCCTATGT CCTCACAGGA TCATTCCAAG
    AACCAGCCCT TTGATGTGAA TACATCTGGA TACTCCCCAC CACACACAGC AGATGCCCTC
    AAGGGTCCAA GGACACATCG GCGCTGCTCA GAGCCCAACA TAGAAGACCA GAACCGCAAG
    CTGACCTATC TCAGGGGAAT TTATTCAAAG AAACAACATA AAACCAGCTG TGAAGCTGGT
    CTCTTGCATG GAGAGGAGGA TTATCTCAAA CGGCATAAGT CTTTGCAAAT GGAGGGGCAG
    AAGCTCATTA ATCAGAGTTT AGTCATGGGG ATTGAGGTGG GCAAGAGTAG TGCCACAAAC
    CAAAACACTG AGAAGGTTTT ACCCCCAAGA TTAAACCTTT GCCCAAGGAC CAGCTATTCC
    AGCTTATCCT CCCCAGGCAC TTCCCCATCC GGCTCATCAG TAAGCTCCCA AGACAGTGCT
    TTTTCTCAGA TTTCTGAACA CTCTGTGTTT ACACCCACTG AGACTTCCTC TCCAATAGAT
    TGCACTTTTC AGGCTCAGAG AAAACGGGAA GACCTTTCTC CTGACTTTAG CAATGCCAGC
    CATGTTTCCG GAATGCCCGG TCCCTCATCA GGGCAGGCTT GCAGCCGCCC AGCCTATACA
    AAGAAGGACA CCATGGAGTG GCATTCACAA ATGCATTCTG TAACTCTTCA TCCCAGCACA
    TGGTTGAGAA ATGGTGTGGC CAGTTTGAAA AACTGGTCCC TCAAAAAGAA AGCAAAGGCA
    GCCAGACCAG AGGAAGAGAA AATAGCTTCT CCAAAAGGAC CCTTAGAGCC ACCCCCACAT
    GCTTCTGGTG TTCCAGAAGC CAACTCACTG CAAGAGGAAC AAAAAGACTT GCCCTTAAGG
    GCAGCTGAAG GACTGTCCCC TGTGCAGTCA GCCCAAAGGT GTAGTTCTTC TCCCTTCCAG
    GACTCAGAGA GACACTGTAG CTCTCCATTC AGCCTGGTGG AGAGCAGACT TAAGCTGTGC
    ATGAAGTCAC ATGAGGAAAT AGAGCCTGGT AGTCAGAGCT CTTCTGGTTC TCTGCCTTGG
    GAAAGAGCCT CAGCCAGCTC TTGGACTCTA GAGGATGCGA CCAGCCCAGA CTCAGGGCCT
    ACAGTGGTCT GCGACATTGA GGACAGGTAT TTAACCAAAG ACATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08022
    Clone ID Related Accession (Same CDS sequence) NM_001258418.1 , NM_001258417.1
    Accession Version NM_001258417.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-24
    Organism Homo sapiens(human)
    Product rho GTPase-activating protein 20 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY496264.1 and AP001883.6. Transcript Variant: This variant (4) differs in the 5' UTR and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 4 and 5 encode the same isoform (4), which has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY496264.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAAACAC TAGCAGAAAG GAGGAGGAGC GCTCCATCTC TTATCCTGGA TAAAGCCCTA 
    CAAAAACGGC CTACTACCAG GGACAGTCCT TCTGCTAGTG TTGACACATG CACATTTCTG
    TCATCATTAG TGTGCTCCAA TAGGACTCTG CTGATTGATG GCCGGGCAGA ACTCAAAAGA
    GGCCTCCAGA GGCAGGAGCG GCATCTTTTC CTATTCAATG ATCTGTTTGT TGTGGCCAAA
    ATCAAATATA ACAATAACTT TAAGATAAAA AATAAAATTA AATTAACTGA TATGTGGACA
    GCAAGCTGTG TGGATGAAGT GGGAGAAGGC AACACCAATG CCATGAAATC CTTTGTTTTG
    GGCTGGCCCA CAGTGAACTT TGTGGCCACT TTCAGTTCTC CAGAACAAAA GGACAAATGG
    CTCTCTCTCC TTCAGAGATA CATCAATCTA GAGAAAGAAA AGGACTACCC GAAGAGCATT
    CCCCTCAAAA TCTTCGCCAA GGACATTGGG AATTGTGCCT ACTCTAAAAC TATAACAGTA
    ATGAATTCAG ATACAGCGAA TGAAGTTATC AACATGTCAT TACCAATGCT AGGGATAACT
    GGCTCTGAGA GAGATTACCA GTTGTGGGTC AATTCTGGCA AAGAAGAGGC TCCATACCCA
    CTCATTGGGC ATGAATATCC ATATGGAATT AAAATGAGCC ATCTTCGAGA CTCTGCACTC
    CTGACACCGG GATCAAAGGA CTCTACCACC CCTTTCAACC TCCAGGAGCC CTTCCTTATG
    GAACAGCTCC CCCGAGAGAT GCAGTGCCAG TTCATCCTGA AGCCCAGCCG CCTGGCTGCA
    GCCCAGCAAC TGAGTGATTC AGGTCATAAG ACATTTAAAA GGAGAAGATC TATCATAAAC
    TGGGCCTTCT GGCGAGGTTC TAGCACTCAC CTGGACAACT TGCCCTCATC GCCAACATCA
    CCTATGCCAG GACAGCTCTT TGGAATTTCT CTGCCAAATA TTTGTGAGAA TGACAATCTG
    CCCAAACCTG TCTTGGATAT GCTTTTCTTT CTTAATCAAA AAGGACCTCT CACCAAAGGT
    ATCTTCAGGC AATCAGCCAA TGTGAAATCC TGCAGAGAAC TAAAAGAGAA ATTGAATTCT
    GGAGTCGAAG TACACCTAGA CTGTGAATCT ATTTTTGTGA TAGCATCTGT CTTAAAGGAT
    TTTCTGCGAA ATATTCCAGG AAGTATTTTT TCATCAGATC TCTATGATCA CTGGGTCTCT
    GTAATGGATC AAGGAAATGA TGAAGAGAAA ATAAATACTG TTCAAAGGCT ATTAGACCAG
    CTTCCGAGAG CCAATGTTGT TCTCCTAAGG TATCTTTTTG GGGTGTTACA CAACATTGAG
    CAACATTCCT CATCCAATCA GATGACTGCA TTTAATTTAG CTGTGTGTGT CGCTCCAAGT
    ATTCTTTGGC CTCCTGCTTC CTCCAGCCCA GAACTAGAAA ACGAATTTAC AAAAAAGGTT
    TCCCTGCTTA TACAATTTCT GATTGAGAAT TGCCTTAGGA TATTTGGAGA AGAAATCACT
    TCCCTCTTCA GAGAGGTTTC AGTGAGATGT GACACTAGAG AGAATGCCTC AGATATTTCT
    TGCTTTCAAC TGAATGACTC CTCCTATGAC AGCTTGGAAA ATGAGCTAAA TGAGGATGTT
    GATGCACCAT GCAGTGACTT GGTAAAGAAA CTTGGCCAGG GGAGCAGAAG CATGGACTCT
    GTCTTAACCC TCAGTGACTA TGATCTTGAC CAGCCCGAGG TGGAAGGCCT TTTAACCCTA
    AGCGACTTTG ACTTGGCCCA TTCTAAAGAT GAAGATGTTC AAATGAAACG GCCTCTTGAA
    TCCAAGCCGG TGAACATTTT AGTGTACACA AAGATCCCAC TGCGGGATCA TGCCAGGGCC
    CCATCTGCCA TGTGCACACC CAGCTACCTG TCCACAGCTG CAGCAAATGC TGCAAAAAGC
    CTGAGGCGAC ACCGGCGTTG CTCAGAGCCC AGCATCGACT ATCTGGATTC AAAGCTTTCC
    TACCTCAGGG AGTTTTATCA GAAAAAGCTA CGCAAGTCCA GCTGTGATGC AATTCTTTCT
    CAAAAAGATG AAGACTATCT GAAGCAGAAT CAACCCCTCC AGGAGGAAGG AAAGACATGT
    TTTAAACAGA GTTTAGTCAC AGGCACTGAT GTCAGCAAGA AAAATGCCAC TACTCAAAAC
    ACTAAGAAGA AAAGCTTGTC TGGTAGTGAA GGAAATCACG TGAAACTTTT CCCTAAGTCT
    AAGCCAGTGG CCATTTCTGT GGCATCTTAT AGTCCTATGT CCTCACAGGA TCATTCCAAG
    AACCAGCCCT TTGATGTGAA TACATCTGGA TACTCCCCAC CACACACAGC AGATGCCCTC
    AAGGGTCCAA GGACACATCG GCGCTGCTCA GAGCCCAACA TAGAAGACCA GAACCGCAAG
    CTGACCTATC TCAGGGGAAT TTATTCAAAG AAACAACATA AAACCAGCTG TGAAGCTGGT
    CTCTTGCATG GAGAGGAGGA TTATCTCAAA CGGCATAAGT CTTTGCAAAT GGAGGGGCAG
    AAGCTCATTA ATCAGAGTTT AGTCATGGGG ATTGAGGTGG GCAAGAGTAG TGCCACAAAC
    CAAAACACTG AGAAGGTTTT ACCCCCAAGA TTAAACCTTT GCCCAAGGAC CAGCTATTCC
    AGCTTATCCT CCCCAGGCAC TTCCCCATCC GGCTCATCAG TAAGCTCCCA AGACAGTGCT
    TTTTCTCAGA TTTCTGAACA CTCTGTGTTT ACACCCACTG AGACTTCCTC TCCAATAGAT
    TGCACTTTTC AGGCTCAGAG AAAACGGGAA GACCTTTCTC CTGACTTTAG CAATGCCAGC
    CATGTTTCCG GAATGCCCGG TCCCTCATCA GGGCAGGCTT GCAGCCGCCC AGCCTATACA
    AAGAAGGACA CCATGGAGTG GCATTCACAA ATGCATTCTG TAACTCTTCA TCCCAGCACA
    TGGTTGAGAA ATGGTGTGGC CAGTTTGAAA AACTGGTCCC TCAAAAAGAA AGCAAAGGCA
    GCCAGACCAG AGGAAGAGAA AATAGCTTCT CCAAAAGGAC CCTTAGAGCC ACCCCCACAT
    GCTTCTGGTG TTCCAGAAGC CAACTCACTG CAAGAGGAAC AAAAAGACTT GCCCTTAAGG
    GCAGCTGAAG GACTGTCCCC TGTGCAGTCA GCCCAAAGGT GTAGTTCTTC TCCCTTCCAG
    GACTCAGAGA GACACTGTAG CTCTCCATTC AGCCTGGTGG AGAGCAGACT TAAGCTGTGC
    ATGAAGTCAC ATGAGGAAAT AGAGCCTGGT AGTCAGAGCT CTTCTGGTTC TCTGCCTTGG
    GAAAGAGCCT CAGCCAGCTC TTGGACTCTA GAGGATGCGA CCAGCCCAGA CTCAGGGCCT
    ACAGTGGTCT GCGACATTGA GGACAGGTAT TTAACCAAAG ACATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245346.1
    CDS97..3564
    Translation

    Target ORF information:

    RefSeq Version NM_001258417.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258417.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAAACAC TAGCAGAAAG GAGGAGGAGC GCTCCATCTC TTATCCTGGA TAAAGCCCTA 
    CAAAAACGGC CTACTACCAG GGACAGTCCT TCTGCTAGTG TTGACACATG CACATTTCTG
    TCATCATTAG TGTGCTCCAA TAGGACTCTG CTGATTGATG GCCGGGCAGA ACTCAAAAGA
    GGCCTCCAGA GGCAGGAGCG GCATCTTTTC CTATTCAATG ATCTGTTTGT TGTGGCCAAA
    ATCAAATATA ACAATAACTT TAAGATAAAA AATAAAATTA AATTAACTGA TATGTGGACA
    GCAAGCTGTG TGGATGAAGT GGGAGAAGGC AACACCAATG CCATGAAATC CTTTGTTTTG
    GGCTGGCCCA CAGTGAACTT TGTGGCCACT TTCAGTTCTC CAGAACAAAA GGACAAATGG
    CTCTCTCTCC TTCAGAGATA CATCAATCTA GAGAAAGAAA AGGACTACCC GAAGAGCATT
    CCCCTCAAAA TCTTCGCCAA GGACATTGGG AATTGTGCCT ACTCTAAAAC TATAACAGTA
    ATGAATTCAG ATACAGCGAA TGAAGTTATC AACATGTCAT TACCAATGCT AGGGATAACT
    GGCTCTGAGA GAGATTACCA GTTGTGGGTC AATTCTGGCA AAGAAGAGGC TCCATACCCA
    CTCATTGGGC ATGAATATCC ATATGGAATT AAAATGAGCC ATCTTCGAGA CTCTGCACTC
    CTGACACCGG GATCAAAGGA CTCTACCACC CCTTTCAACC TCCAGGAGCC CTTCCTTATG
    GAACAGCTCC CCCGAGAGAT GCAGTGCCAG TTCATCCTGA AGCCCAGCCG CCTGGCTGCA
    GCCCAGCAAC TGAGTGATTC AGGTCATAAG ACATTTAAAA GGAGAAGATC TATCATAAAC
    TGGGCCTTCT GGCGAGGTTC TAGCACTCAC CTGGACAACT TGCCCTCATC GCCAACATCA
    CCTATGCCAG GACAGCTCTT TGGAATTTCT CTGCCAAATA TTTGTGAGAA TGACAATCTG
    CCCAAACCTG TCTTGGATAT GCTTTTCTTT CTTAATCAAA AAGGACCTCT CACCAAAGGT
    ATCTTCAGGC AATCAGCCAA TGTGAAATCC TGCAGAGAAC TAAAAGAGAA ATTGAATTCT
    GGAGTCGAAG TACACCTAGA CTGTGAATCT ATTTTTGTGA TAGCATCTGT CTTAAAGGAT
    TTTCTGCGAA ATATTCCAGG AAGTATTTTT TCATCAGATC TCTATGATCA CTGGGTCTCT
    GTAATGGATC AAGGAAATGA TGAAGAGAAA ATAAATACTG TTCAAAGGCT ATTAGACCAG
    CTTCCGAGAG CCAATGTTGT TCTCCTAAGG TATCTTTTTG GGGTGTTACA CAACATTGAG
    CAACATTCCT CATCCAATCA GATGACTGCA TTTAATTTAG CTGTGTGTGT CGCTCCAAGT
    ATTCTTTGGC CTCCTGCTTC CTCCAGCCCA GAACTAGAAA ACGAATTTAC AAAAAAGGTT
    TCCCTGCTTA TACAATTTCT GATTGAGAAT TGCCTTAGGA TATTTGGAGA AGAAATCACT
    TCCCTCTTCA GAGAGGTTTC AGTGAGATGT GACACTAGAG AGAATGCCTC AGATATTTCT
    TGCTTTCAAC TGAATGACTC CTCCTATGAC AGCTTGGAAA ATGAGCTAAA TGAGGATGTT
    GATGCACCAT GCAGTGACTT GGTAAAGAAA CTTGGCCAGG GGAGCAGAAG CATGGACTCT
    GTCTTAACCC TCAGTGACTA TGATCTTGAC CAGCCCGAGG TGGAAGGCCT TTTAACCCTA
    AGCGACTTTG ACTTGGCCCA TTCTAAAGAT GAAGATGTTC AAATGAAACG GCCTCTTGAA
    TCCAAGCCGG TGAACATTTT AGTGTACACA AAGATCCCAC TGCGGGATCA TGCCAGGGCC
    CCATCTGCCA TGTGCACACC CAGCTACCTG TCCACAGCTG CAGCAAATGC TGCAAAAAGC
    CTGAGGCGAC ACCGGCGTTG CTCAGAGCCC AGCATCGACT ATCTGGATTC AAAGCTTTCC
    TACCTCAGGG AGTTTTATCA GAAAAAGCTA CGCAAGTCCA GCTGTGATGC AATTCTTTCT
    CAAAAAGATG AAGACTATCT GAAGCAGAAT CAACCCCTCC AGGAGGAAGG AAAGACATGT
    TTTAAACAGA GTTTAGTCAC AGGCACTGAT GTCAGCAAGA AAAATGCCAC TACTCAAAAC
    ACTAAGAAGA AAAGCTTGTC TGGTAGTGAA GGAAATCACG TGAAACTTTT CCCTAAGTCT
    AAGCCAGTGG CCATTTCTGT GGCATCTTAT AGTCCTATGT CCTCACAGGA TCATTCCAAG
    AACCAGCCCT TTGATGTGAA TACATCTGGA TACTCCCCAC CACACACAGC AGATGCCCTC
    AAGGGTCCAA GGACACATCG GCGCTGCTCA GAGCCCAACA TAGAAGACCA GAACCGCAAG
    CTGACCTATC TCAGGGGAAT TTATTCAAAG AAACAACATA AAACCAGCTG TGAAGCTGGT
    CTCTTGCATG GAGAGGAGGA TTATCTCAAA CGGCATAAGT CTTTGCAAAT GGAGGGGCAG
    AAGCTCATTA ATCAGAGTTT AGTCATGGGG ATTGAGGTGG GCAAGAGTAG TGCCACAAAC
    CAAAACACTG AGAAGGTTTT ACCCCCAAGA TTAAACCTTT GCCCAAGGAC CAGCTATTCC
    AGCTTATCCT CCCCAGGCAC TTCCCCATCC GGCTCATCAG TAAGCTCCCA AGACAGTGCT
    TTTTCTCAGA TTTCTGAACA CTCTGTGTTT ACACCCACTG AGACTTCCTC TCCAATAGAT
    TGCACTTTTC AGGCTCAGAG AAAACGGGAA GACCTTTCTC CTGACTTTAG CAATGCCAGC
    CATGTTTCCG GAATGCCCGG TCCCTCATCA GGGCAGGCTT GCAGCCGCCC AGCCTATACA
    AAGAAGGACA CCATGGAGTG GCATTCACAA ATGCATTCTG TAACTCTTCA TCCCAGCACA
    TGGTTGAGAA ATGGTGTGGC CAGTTTGAAA AACTGGTCCC TCAAAAAGAA AGCAAAGGCA
    GCCAGACCAG AGGAAGAGAA AATAGCTTCT CCAAAAGGAC CCTTAGAGCC ACCCCCACAT
    GCTTCTGGTG TTCCAGAAGC CAACTCACTG CAAGAGGAAC AAAAAGACTT GCCCTTAAGG
    GCAGCTGAAG GACTGTCCCC TGTGCAGTCA GCCCAAAGGT GTAGTTCTTC TCCCTTCCAG
    GACTCAGAGA GACACTGTAG CTCTCCATTC AGCCTGGTGG AGAGCAGACT TAAGCTGTGC
    ATGAAGTCAC ATGAGGAAAT AGAGCCTGGT AGTCAGAGCT CTTCTGGTTC TCTGCCTTGG
    GAAAGAGCCT CAGCCAGCTC TTGGACTCTA GAGGATGCGA CCAGCCCAGA CTCAGGGCCT
    ACAGTGGTCT GCGACATTGA GGACAGGTAT TTAACCAAAG ACATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08682
    Clone ID Related Accession (Same CDS sequence) XM_006718893.3 , NM_020809.3
    Accession Version NM_020809.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product rho GTPase-activating protein 20 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY496263.1 and AP001883.6. This sequence is a reference standard in the RefSeqGene project. On May 2, 2012 this sequence version replaced NM_020809.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY496263.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAGCGA TGTCCCCCCA GCAGGAGACT CTAGGGGGAC AGCCGGGGCG CTCCTCTTCC 
    CTGACAGGAG TGTCTCGGCT CGCGGGAGGC AGCTGCACCA AGAAGAAAAT GAAAACACTA
    GCAGAAAGGA GGAGGAGCGC TCCATCTCTT ATCCTGGATA AAGCCCTACA AAAACGGCCT
    ACTACCAGGG ACAGTCCTTC TGCTAGTGTT GACACATGCA CATTTCTGTC ATCATTAGTG
    TGCTCCAATA GGACTCTGCT GATTGATGGC CGGGCAGAAC TCAAAAGAGG CCTCCAGAGG
    CAGGAGCGGC ATCTTTTCCT ATTCAATGAT CTGTTTGTTG TGGCCAAAAT CAAATATAAC
    AATAACTTTA AGATAAAAAA TAAAATTAAA TTAACTGATA TGTGGACAGC AAGCTGTGTG
    GATGAAGTGG GAGAAGGCAA CACCAATGCC ATGAAATCCT TTGTTTTGGG CTGGCCCACA
    GTGAACTTTG TGGCCACTTT CAGTTCTCCA GAACAAAAGG ACAAATGGCT CTCTCTCCTT
    CAGAGATACA TCAATCTAGA GAAAGAAAAG GACTACCCGA AGAGCATTCC CCTCAAAATC
    TTCGCCAAGG ACATTGGGAA TTGTGCCTAC TCTAAAACTA TAACAGTAAT GAATTCAGAT
    ACAGCGAATG AAGTTATCAA CATGTCATTA CCAATGCTAG GGATAACTGG CTCTGAGAGA
    GATTACCAGT TGTGGGTCAA TTCTGGCAAA GAAGAGGCTC CATACCCACT CATTGGGCAT
    GAATATCCAT ATGGAATTAA AATGAGCCAT CTTCGAGACT CTGCACTCCT GACACCGGGA
    TCAAAGGACT CTACCACCCC TTTCAACCTC CAGGAGCCCT TCCTTATGGA ACAGCTCCCC
    CGAGAGATGC AGTGCCAGTT CATCCTGAAG CCCAGCCGCC TGGCTGCAGC CCAGCAACTG
    AGTGATTCAG GTCATAAGAC ATTTAAAAGG AGAAGATCTA TCATAAACTG GGCCTTCTGG
    CGAGGTTCTA GCACTCACCT GGACAACTTG CCCTCATCGC CAACATCACC TATGCCAGGA
    CAGCTCTTTG GAATTTCTCT GCCAAATATT TGTGAGAATG ACAATCTGCC CAAACCTGTC
    TTGGATATGC TTTTCTTTCT TAATCAAAAA GGACCTCTCA CCAAAGGTAT CTTCAGGCAA
    TCAGCCAATG TGAAATCCTG CAGAGAACTA AAAGAGAAAT TGAATTCTGG AGTCGAAGTA
    CACCTAGACT GTGAATCTAT TTTTGTGATA GCATCTGTCT TAAAGGATTT TCTGCGAAAT
    ATTCCAGGAA GTATTTTTTC ATCAGATCTC TATGATCACT GGGTCTCTGT AATGGATCAA
    GGAAATGATG AAGAGAAAAT AAATACTGTT CAAAGGCTAT TAGACCAGCT TCCGAGAGCC
    AATGTTGTTC TCCTAAGGTA TCTTTTTGGG GTGTTACACA ACATTGAGCA ACATTCCTCA
    TCCAATCAGA TGACTGCATT TAATTTAGCT GTGTGTGTCG CTCCAAGTAT TCTTTGGCCT
    CCTGCTTCCT CCAGCCCAGA ACTAGAAAAC GAATTTACAA AAAAGGTTTC CCTGCTTATA
    CAATTTCTGA TTGAGAATTG CCTTAGGATA TTTGGAGAAG AAATCACTTC CCTCTTCAGA
    GAGGTTTCAG TGAGATGTGA CACTAGAGAG AATGCCTCAG ATATTTCTTG CTTTCAACTG
    AATGACTCCT CCTATGACAG CTTGGAAAAT GAGCTAAATG AGGATGTTGA TGCACCATGC
    AGTGACTTGG TAAAGAAACT TGGCCAGGGG AGCAGAAGCA TGGACTCTGT CTTAACCCTC
    AGTGACTATG ATCTTGACCA GCCCGAGGTG GAAGGCCTTT TAACCCTAAG CGACTTTGAC
    TTGGCCCATT CTAAAGATGA AGATGTTCAA ATGAAACGGC CTCTTGAATC CAAGCCGGTG
    AACATTTTAG TGTACACAAA GATCCCACTG CGGGATCATG CCAGGGCCCC ATCTGCCATG
    TGCACACCCA GCTACCTGTC CACAGCTGCA GCAAATGCTG CAAAAAGCCT GAGGCGACAC
    CGGCGTTGCT CAGAGCCCAG CATCGACTAT CTGGATTCAA AGCTTTCCTA CCTCAGGGAG
    TTTTATCAGA AAAAGCTACG CAAGTCCAGC TGTGATGCAA TTCTTTCTCA AAAAGATGAA
    GACTATCTGA AGCAGAATCA ACCCCTCCAG GAGGAAGGAA AGACATGTTT TAAACAGAGT
    TTAGTCACAG GCACTGATGT CAGCAAGAAA AATGCCACTA CTCAAAACAC TAAGAAGAAA
    AGCTTGTCTG GTAGTGAAGG AAATCACGTG AAACTTTTCC CTAAGTCTAA GCCAGTGGCC
    ATTTCTGTGG CATCTTATAG TCCTATGTCC TCACAGGATC ATTCCAAGAA CCAGCCCTTT
    GATGTGAATA CATCTGGATA CTCCCCACCA CACACAGCAG ATGCCCTCAA GGGTCCAAGG
    ACACATCGGC GCTGCTCAGA GCCCAACATA GAAGACCAGA ACCGCAAGCT GACCTATCTC
    AGGGGAATTT ATTCAAAGAA ACAACATAAA ACCAGCTGTG AAGCTGGTCT CTTGCATGGA
    GAGGAGGATT ATCTCAAACG GCATAAGTCT TTGCAAATGG AGGGGCAGAA GCTCATTAAT
    CAGAGTTTAG TCATGGGGAT TGAGGTGGGC AAGAGTAGTG CCACAAACCA AAACACTGAG
    AAGGTTTTAC CCCCAAGATT AAACCTTTGC CCAAGGACCA GCTATTCCAG CTTATCCTCC
    CCAGGCACTT CCCCATCCGG CTCATCAGTA AGCTCCCAAG ACAGTGCTTT TTCTCAGATT
    TCTGAACACT CTGTGTTTAC ACCCACTGAG ACTTCCTCTC CAATAGATTG CACTTTTCAG
    GCTCAGAGAA AACGGGAAGA CCTTTCTCCT GACTTTAGCA ATGCCAGCCA TGTTTCCGGA
    ATGCCCGGTC CCTCATCAGG GCAGGCTTGC AGCCGCCCAG CCTATACAAA GAAGGACACC
    ATGGAGTGGC ATTCACAAAT GCATTCTGTA ACTCTTCATC CCAGCACATG GTTGAGAAAT
    GGTGTGGCCA GTTTGAAAAA CTGGTCCCTC AAAAAGAAAG CAAAGGCAGC CAGACCAGAG
    GAAGAGAAAA TAGCTTCTCC AAAAGGACCC TTAGAGCCAC CCCCACATGC TTCTGGTGTT
    CCAGAAGCCA ACTCACTGCA AGAGGAACAA AAAGACTTGC CCTTAAGGGC AGCTGAAGGA
    CTGTCCCCTG TGCAGTCAGC CCAAAGGTGT AGTTCTTCTC CCTTCCAGGA CTCAGAGAGA
    CACTGTAGCT CTCCATTCAG CCTGGTGGAG AGCAGACTTA AGCTGTGCAT GAAGTCACAT
    GAGGAAATAG AGCCTGGTAG TCAGAGCTCT TCTGGTTCTC TGCCTTGGGA AAGAGCCTCA
    GCCAGCTCTT GGACTCTAGA GGATGCGACC AGCCCAGACT CAGGGCCTAC AGTGGTCTGC
    GACATTGAGG ACAGGTATTT AACCAAAGAC ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_065860.2
    CDS286..3861
    Translation

    Target ORF information:

    RefSeq Version NM_020809.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020809.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAGCGA TGTCCCCCCA GCAGGAGACT CTAGGGGGAC AGCCGGGGCG CTCCTCTTCC 
    CTGACAGGAG TGTCTCGGCT CGCGGGAGGC AGCTGCACCA AGAAGAAAAT GAAAACACTA
    GCAGAAAGGA GGAGGAGCGC TCCATCTCTT ATCCTGGATA AAGCCCTACA AAAACGGCCT
    ACTACCAGGG ACAGTCCTTC TGCTAGTGTT GACACATGCA CATTTCTGTC ATCATTAGTG
    TGCTCCAATA GGACTCTGCT GATTGATGGC CGGGCAGAAC TCAAAAGAGG CCTCCAGAGG
    CAGGAGCGGC ATCTTTTCCT ATTCAATGAT CTGTTTGTTG TGGCCAAAAT CAAATATAAC
    AATAACTTTA AGATAAAAAA TAAAATTAAA TTAACTGATA TGTGGACAGC AAGCTGTGTG
    GATGAAGTGG GAGAAGGCAA CACCAATGCC ATGAAATCCT TTGTTTTGGG CTGGCCCACA
    GTGAACTTTG TGGCCACTTT CAGTTCTCCA GAACAAAAGG ACAAATGGCT CTCTCTCCTT
    CAGAGATACA TCAATCTAGA GAAAGAAAAG GACTACCCGA AGAGCATTCC CCTCAAAATC
    TTCGCCAAGG ACATTGGGAA TTGTGCCTAC TCTAAAACTA TAACAGTAAT GAATTCAGAT
    ACAGCGAATG AAGTTATCAA CATGTCATTA CCAATGCTAG GGATAACTGG CTCTGAGAGA
    GATTACCAGT TGTGGGTCAA TTCTGGCAAA GAAGAGGCTC CATACCCACT CATTGGGCAT
    GAATATCCAT ATGGAATTAA AATGAGCCAT CTTCGAGACT CTGCACTCCT GACACCGGGA
    TCAAAGGACT CTACCACCCC TTTCAACCTC CAGGAGCCCT TCCTTATGGA ACAGCTCCCC
    CGAGAGATGC AGTGCCAGTT CATCCTGAAG CCCAGCCGCC TGGCTGCAGC CCAGCAACTG
    AGTGATTCAG GTCATAAGAC ATTTAAAAGG AGAAGATCTA TCATAAACTG GGCCTTCTGG
    CGAGGTTCTA GCACTCACCT GGACAACTTG CCCTCATCGC CAACATCACC TATGCCAGGA
    CAGCTCTTTG GAATTTCTCT GCCAAATATT TGTGAGAATG ACAATCTGCC CAAACCTGTC
    TTGGATATGC TTTTCTTTCT TAATCAAAAA GGACCTCTCA CCAAAGGTAT CTTCAGGCAA
    TCAGCCAATG TGAAATCCTG CAGAGAACTA AAAGAGAAAT TGAATTCTGG AGTCGAAGTA
    CACCTAGACT GTGAATCTAT TTTTGTGATA GCATCTGTCT TAAAGGATTT TCTGCGAAAT
    ATTCCAGGAA GTATTTTTTC ATCAGATCTC TATGATCACT GGGTCTCTGT AATGGATCAA
    GGAAATGATG AAGAGAAAAT AAATACTGTT CAAAGGCTAT TAGACCAGCT TCCGAGAGCC
    AATGTTGTTC TCCTAAGGTA TCTTTTTGGG GTGTTACACA ACATTGAGCA ACATTCCTCA
    TCCAATCAGA TGACTGCATT TAATTTAGCT GTGTGTGTCG CTCCAAGTAT TCTTTGGCCT
    CCTGCTTCCT CCAGCCCAGA ACTAGAAAAC GAATTTACAA AAAAGGTTTC CCTGCTTATA
    CAATTTCTGA TTGAGAATTG CCTTAGGATA TTTGGAGAAG AAATCACTTC CCTCTTCAGA
    GAGGTTTCAG TGAGATGTGA CACTAGAGAG AATGCCTCAG ATATTTCTTG CTTTCAACTG
    AATGACTCCT CCTATGACAG CTTGGAAAAT GAGCTAAATG AGGATGTTGA TGCACCATGC
    AGTGACTTGG TAAAGAAACT TGGCCAGGGG AGCAGAAGCA TGGACTCTGT CTTAACCCTC
    AGTGACTATG ATCTTGACCA GCCCGAGGTG GAAGGCCTTT TAACCCTAAG CGACTTTGAC
    TTGGCCCATT CTAAAGATGA AGATGTTCAA ATGAAACGGC CTCTTGAATC CAAGCCGGTG
    AACATTTTAG TGTACACAAA GATCCCACTG CGGGATCATG CCAGGGCCCC ATCTGCCATG
    TGCACACCCA GCTACCTGTC CACAGCTGCA GCAAATGCTG CAAAAAGCCT GAGGCGACAC
    CGGCGTTGCT CAGAGCCCAG CATCGACTAT CTGGATTCAA AGCTTTCCTA CCTCAGGGAG
    TTTTATCAGA AAAAGCTACG CAAGTCCAGC TGTGATGCAA TTCTTTCTCA AAAAGATGAA
    GACTATCTGA AGCAGAATCA ACCCCTCCAG GAGGAAGGAA AGACATGTTT TAAACAGAGT
    TTAGTCACAG GCACTGATGT CAGCAAGAAA AATGCCACTA CTCAAAACAC TAAGAAGAAA
    AGCTTGTCTG GTAGTGAAGG AAATCACGTG AAACTTTTCC CTAAGTCTAA GCCAGTGGCC
    ATTTCTGTGG CATCTTATAG TCCTATGTCC TCACAGGATC ATTCCAAGAA CCAGCCCTTT
    GATGTGAATA CATCTGGATA CTCCCCACCA CACACAGCAG ATGCCCTCAA GGGTCCAAGG
    ACACATCGGC GCTGCTCAGA GCCCAACATA GAAGACCAGA ACCGCAAGCT GACCTATCTC
    AGGGGAATTT ATTCAAAGAA ACAACATAAA ACCAGCTGTG AAGCTGGTCT CTTGCATGGA
    GAGGAGGATT ATCTCAAACG GCATAAGTCT TTGCAAATGG AGGGGCAGAA GCTCATTAAT
    CAGAGTTTAG TCATGGGGAT TGAGGTGGGC AAGAGTAGTG CCACAAACCA AAACACTGAG
    AAGGTTTTAC CCCCAAGATT AAACCTTTGC CCAAGGACCA GCTATTCCAG CTTATCCTCC
    CCAGGCACTT CCCCATCCGG CTCATCAGTA AGCTCCCAAG ACAGTGCTTT TTCTCAGATT
    TCTGAACACT CTGTGTTTAC ACCCACTGAG ACTTCCTCTC CAATAGATTG CACTTTTCAG
    GCTCAGAGAA AACGGGAAGA CCTTTCTCCT GACTTTAGCA ATGCCAGCCA TGTTTCCGGA
    ATGCCCGGTC CCTCATCAGG GCAGGCTTGC AGCCGCCCAG CCTATACAAA GAAGGACACC
    ATGGAGTGGC ATTCACAAAT GCATTCTGTA ACTCTTCATC CCAGCACATG GTTGAGAAAT
    GGTGTGGCCA GTTTGAAAAA CTGGTCCCTC AAAAAGAAAG CAAAGGCAGC CAGACCAGAG
    GAAGAGAAAA TAGCTTCTCC AAAAGGACCC TTAGAGCCAC CCCCACATGC TTCTGGTGTT
    CCAGAAGCCA ACTCACTGCA AGAGGAACAA AAAGACTTGC CCTTAAGGGC AGCTGAAGGA
    CTGTCCCCTG TGCAGTCAGC CCAAAGGTGT AGTTCTTCTC CCTTCCAGGA CTCAGAGAGA
    CACTGTAGCT CTCCATTCAG CCTGGTGGAG AGCAGACTTA AGCTGTGCAT GAAGTCACAT
    GAGGAAATAG AGCCTGGTAG TCAGAGCTCT TCTGGTTCTC TGCCTTGGGA AAGAGCCTCA
    GCCAGCTCTT GGACTCTAGA GGATGCGACC AGCCCAGACT CAGGGCCTAC AGTGGTCTGC
    GACATTGAGG ACAGGTATTT AACCAAAGAC ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12330
    Clone ID Related Accession (Same CDS sequence) NM_001258415.2 , NM_001258415.1
    Accession Version NM_001258415.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-01
    Organism Homo sapiens(human)
    Product rho GTPase-activating protein 20 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY496266.1 and AP001883.6. On Jun 2, 2019 this sequence version replaced NM_001258415.1. Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY496266.1, SRR1803616.252833.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCTGCCC GGGAGCGGCA GCCGGCGCTG AAGAAGAAAA TGAAAACACT AGCAGAAAGG 
    AGGAGGAGCG CTCCATCTCT TATCCTGGAT AAAGCCCTAC AAAAACGGCC TACTACCAGG
    GACAGTCCTT CTGCTAGTGT TGACACATGC ACATTTCTGT CATCATTAGT GTGCTCCAAT
    AGGACTCTGC TGATTGATGG CCGGGCAGAA CTCAAAAGAG GCCTCCAGAG GCAGGAGCGG
    CATCTTTTCC TATTCAATGA TCTGTTTGTT GTGGCCAAAA TCAAATATAA CAATAACTTT
    AAGATAAAAA ATAAAATTAA ATTAACTGAT ATGTGGACAG CAAGCTGTGT GGATGAAGTG
    GGAGAAGGCA ACACCAATGC CATGAAATCC TTTGTTTTGG GCTGGCCCAC AGTGAACTTT
    GTGGCCACTT TCAGTTCTCC AGAACAAAAG GACAAATGGC TCTCTCTCCT TCAGAGATAC
    ATCAATCTAG AGAAAGAAAA GGACTACCCG AAGAGCATTC CCCTCAAAAT CTTCGCCAAG
    GACATTGGGA ATTGTGCCTA CTCTAAAACT ATAACAGTAA TGAATTCAGA TACAGCGAAT
    GAAGTTATCA ACATGTCATT ACCAATGCTA GGGATAACTG GCTCTGAGAG AGATTACCAG
    TTGTGGGTCA ATTCTGGCAA AGAAGAGGCT CCATACCCAC TCATTGGGCA TGAATATCCA
    TATGGAATTA AAATGAGCCA TCTTCGAGAC TCTGCACTCC TGACACCGGG ATCAAAGGAC
    TCTACCACCC CTTTCAACCT CCAGGAGCCC TTCCTTATGG AACAGCTCCC CCGAGAGATG
    CAGTGCCAGT TCATCCTGAA GCCCAGCCGC CTGGCTGCAG CCCAGCAACT GAGTGATTCA
    GGTCATAAGA CATTTAAAAG GAGAAGATCT ATCATAAACT GGGCCTTCTG GCGAGGTTCT
    AGCACTCACC TGGACAACTT GCCCTCATCG CCAACATCAC CTATGCCAGG ACAGCTCTTT
    GGAATTTCTC TGCCAAATAT TTGTGAGAAT GACAATCTGC CCAAACCTGT CTTGGATATG
    CTTTTCTTTC TTAATCAAAA AGGACCTCTC ACCAAAGGTA TCTTCAGGCA ATCAGCCAAT
    GTGAAATCCT GCAGAGAACT AAAAGAGAAA TTGAATTCTG GAGTCGAAGT ACACCTAGAC
    TGTGAATCTA TTTTTGTGAT AGCATCTGTC TTAAAGGATT TTCTGCGAAA TATTCCAGGA
    AGTATTTTTT CATCAGATCT CTATGATCAC TGGGTCTCTG TAATGGATCA AGGAAATGAT
    GAAGAGAAAA TAAATACTGT TCAAAGGCTA TTAGACCAGC TTCCGAGAGC CAATGTTGTT
    CTCCTAAGGT ATCTTTTTGG GGTGTTACAC AACATTGAGC AACATTCCTC ATCCAATCAG
    ATGACTGCAT TTAATTTAGC TGTGTGTGTC GCTCCAAGTA TTCTTTGGCC TCCTGCTTCC
    TCCAGCCCAG AACTAGAAAA CGAATTTACA AAAAAGGTTT CCCTGCTTAT ACAATTTCTG
    ATTGAGAATT GCCTTAGGAT ATTTGGAGAA GAAATCACTT CCCTCTTCAG AGAGGTTTCA
    GTGAGATGTG ACACTAGAGA GAATGCCTCA GATATTTCTT GCTTTCAACT GAATGACTCC
    TCCTATGACA GCTTGGAAAA TGAGCTAAAT GAGGATGTTG ATGCACCATG CAGTGACTTG
    GTAAAGAAAC TTGGCCAGGG GAGCAGAAGC ATGGACTCTG TCTTAACCCT CAGTGACTAT
    GATCTTGACC AGCCCGAGGT GGAAGGCCTT TTAACCCTAA GCGACTTTGA CTTGGCCCAT
    TCTAAAGATG AAGATGTTCA AATGAAACGG CCTCTTGAAT CCAAGCCGGT GAACATTTTA
    GTGTACACAA AGATCCCACT GCGGGATCAT GCCAGGGCCC CATCTGCCAT GTGCACACCC
    AGCTACCTGT CCACAGCTGC AGCAAATGCT GCAAAAAGCC TGAGGCGACA CCGGCGTTGC
    TCAGAGCCCA GCATCGACTA TCTGGATTCA AAGCTTTCCT ACCTCAGGGA GTTTTATCAG
    AAAAAGCTAC GCAAGTCCAG CTGTGATGCA ATTCTTTCTC AAAAAGATGA AGACTATCTG
    AAGCAGAATC AACCCCTCCA GGAGGAAGGA AAGACATGTT TTAAACAGAG TTTAGTCACA
    GGCACTGATG TCAGCAAGAA AAATGCCACT ACTCAAAACA CTAAGAAGAA AAGCTTGTCT
    GGTAGTGAAG GAAATCACGT GAAACTTTTC CCTAAGTCTA AGCCAGTGGC CATTTCTGTG
    GCATCTTATA GTCCTATGTC CTCACAGGAT CATTCCAAGA ACCAGCCCTT TGATGTGAAT
    ACATCTGGAT ACTCCCCACC ACACACAGCA GATGCCCTCA AGGGTCCAAG GACACATCGG
    CGCTGCTCAG AGCCCAACAT AGAAGACCAG AACCGCAAGC TGACCTATCT CAGGGGAATT
    TATTCAAAGA AACAACATAA AACCAGCTGT GAAGCTGGTC TCTTGCATGG AGAGGAGGAT
    TATCTCAAAC GGCATAAGTC TTTGCAAATG GAGGGGCAGA AGCTCATTAA TCAGAGTTTA
    GTCATGGGGA TTGAGGTGGG CAAGAGTAGT GCCACAAACC AAAACACTGA GAAGGTTTTA
    CCCCCAAGAT TAAACCTTTG CCCAAGGACC AGCTATTCCA GCTTATCCTC CCCAGGCACT
    TCCCCATCCG GCTCATCAGT AAGCTCCCAA GACAGTGCTT TTTCTCAGAT TTCTGAACAC
    TCTGTGTTTA CACCCACTGA GACTTCCTCT CCAATAGATT GCACTTTTCA GGCTCAGAGA
    AAACGGGAAG ACCTTTCTCC TGACTTTAGC AATGCCAGCC ATGTTTCCGG AATGCCCGGT
    CCCTCATCAG GGCAGGCTTG CAGCCGCCCA GCCTATACAA AGAAGGACAC CATGGAGTGG
    CATTCACAAA TGCATTCTGT AACTCTTCAT CCCAGCACAT GGTTGAGAAA TGGTGTGGCC
    AGTTTGAAAA ACTGGTCCCT CAAAAAGAAA GCAAAGGCAG CCAGACCAGA GGAAGAGAAA
    ATAGCTTCTC CAAAAGGACC CTTAGAGCCA CCCCCACATG CTTCTGGTGT TCCAGAAGCC
    AACTCACTGC AAGAGGAACA AAAAGACTTG CCCTTAAGGG CAGCTGAAGG ACTGTCCCCT
    GTGCAGTCAG CCCAAAGGTG TAGTTCTTCT CCCTTCCAGG ACTCAGAGAG ACACTGTAGC
    TCTCCATTCA GCCTGGTGGA GAGCAGACTT AAGCTGTGCA TGAAGTCACA TGAGGAAATA
    GAGCCTGGTA GTCAGAGCTC TTCTGGTTCT CTGCCTTGGG AAAGAGCCTC AGCCAGCTCT
    TGGACTCTAG AGGATGCGAC CAGCCCAGAC TCAGGGCCTA CAGTGGTCTG CGACATTGAG
    GACAGGTATT TAACCAAAGA CATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245344.1
    CDS133..3639
    Translation

    Target ORF information:

    RefSeq Version NM_001258415.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258415.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCTGCCC GGGAGCGGCA GCCGGCGCTG AAGAAGAAAA TGAAAACACT AGCAGAAAGG 
    AGGAGGAGCG CTCCATCTCT TATCCTGGAT AAAGCCCTAC AAAAACGGCC TACTACCAGG
    GACAGTCCTT CTGCTAGTGT TGACACATGC ACATTTCTGT CATCATTAGT GTGCTCCAAT
    AGGACTCTGC TGATTGATGG CCGGGCAGAA CTCAAAAGAG GCCTCCAGAG GCAGGAGCGG
    CATCTTTTCC TATTCAATGA TCTGTTTGTT GTGGCCAAAA TCAAATATAA CAATAACTTT
    AAGATAAAAA ATAAAATTAA ATTAACTGAT ATGTGGACAG CAAGCTGTGT GGATGAAGTG
    GGAGAAGGCA ACACCAATGC CATGAAATCC TTTGTTTTGG GCTGGCCCAC AGTGAACTTT
    GTGGCCACTT TCAGTTCTCC AGAACAAAAG GACAAATGGC TCTCTCTCCT TCAGAGATAC
    ATCAATCTAG AGAAAGAAAA GGACTACCCG AAGAGCATTC CCCTCAAAAT CTTCGCCAAG
    GACATTGGGA ATTGTGCCTA CTCTAAAACT ATAACAGTAA TGAATTCAGA TACAGCGAAT
    GAAGTTATCA ACATGTCATT ACCAATGCTA GGGATAACTG GCTCTGAGAG AGATTACCAG
    TTGTGGGTCA ATTCTGGCAA AGAAGAGGCT CCATACCCAC TCATTGGGCA TGAATATCCA
    TATGGAATTA AAATGAGCCA TCTTCGAGAC TCTGCACTCC TGACACCGGG ATCAAAGGAC
    TCTACCACCC CTTTCAACCT CCAGGAGCCC TTCCTTATGG AACAGCTCCC CCGAGAGATG
    CAGTGCCAGT TCATCCTGAA GCCCAGCCGC CTGGCTGCAG CCCAGCAACT GAGTGATTCA
    GGTCATAAGA CATTTAAAAG GAGAAGATCT ATCATAAACT GGGCCTTCTG GCGAGGTTCT
    AGCACTCACC TGGACAACTT GCCCTCATCG CCAACATCAC CTATGCCAGG ACAGCTCTTT
    GGAATTTCTC TGCCAAATAT TTGTGAGAAT GACAATCTGC CCAAACCTGT CTTGGATATG
    CTTTTCTTTC TTAATCAAAA AGGACCTCTC ACCAAAGGTA TCTTCAGGCA ATCAGCCAAT
    GTGAAATCCT GCAGAGAACT AAAAGAGAAA TTGAATTCTG GAGTCGAAGT ACACCTAGAC
    TGTGAATCTA TTTTTGTGAT AGCATCTGTC TTAAAGGATT TTCTGCGAAA TATTCCAGGA
    AGTATTTTTT CATCAGATCT CTATGATCAC TGGGTCTCTG TAATGGATCA AGGAAATGAT
    GAAGAGAAAA TAAATACTGT TCAAAGGCTA TTAGACCAGC TTCCGAGAGC CAATGTTGTT
    CTCCTAAGGT ATCTTTTTGG GGTGTTACAC AACATTGAGC AACATTCCTC ATCCAATCAG
    ATGACTGCAT TTAATTTAGC TGTGTGTGTC GCTCCAAGTA TTCTTTGGCC TCCTGCTTCC
    TCCAGCCCAG AACTAGAAAA CGAATTTACA AAAAAGGTTT CCCTGCTTAT ACAATTTCTG
    ATTGAGAATT GCCTTAGGAT ATTTGGAGAA GAAATCACTT CCCTCTTCAG AGAGGTTTCA
    GTGAGATGTG ACACTAGAGA GAATGCCTCA GATATTTCTT GCTTTCAACT GAATGACTCC
    TCCTATGACA GCTTGGAAAA TGAGCTAAAT GAGGATGTTG ATGCACCATG CAGTGACTTG
    GTAAAGAAAC TTGGCCAGGG GAGCAGAAGC ATGGACTCTG TCTTAACCCT CAGTGACTAT
    GATCTTGACC AGCCCGAGGT GGAAGGCCTT TTAACCCTAA GCGACTTTGA CTTGGCCCAT
    TCTAAAGATG AAGATGTTCA AATGAAACGG CCTCTTGAAT CCAAGCCGGT GAACATTTTA
    GTGTACACAA AGATCCCACT GCGGGATCAT GCCAGGGCCC CATCTGCCAT GTGCACACCC
    AGCTACCTGT CCACAGCTGC AGCAAATGCT GCAAAAAGCC TGAGGCGACA CCGGCGTTGC
    TCAGAGCCCA GCATCGACTA TCTGGATTCA AAGCTTTCCT ACCTCAGGGA GTTTTATCAG
    AAAAAGCTAC GCAAGTCCAG CTGTGATGCA ATTCTTTCTC AAAAAGATGA AGACTATCTG
    AAGCAGAATC AACCCCTCCA GGAGGAAGGA AAGACATGTT TTAAACAGAG TTTAGTCACA
    GGCACTGATG TCAGCAAGAA AAATGCCACT ACTCAAAACA CTAAGAAGAA AAGCTTGTCT
    GGTAGTGAAG GAAATCACGT GAAACTTTTC CCTAAGTCTA AGCCAGTGGC CATTTCTGTG
    GCATCTTATA GTCCTATGTC CTCACAGGAT CATTCCAAGA ACCAGCCCTT TGATGTGAAT
    ACATCTGGAT ACTCCCCACC ACACACAGCA GATGCCCTCA AGGGTCCAAG GACACATCGG
    CGCTGCTCAG AGCCCAACAT AGAAGACCAG AACCGCAAGC TGACCTATCT CAGGGGAATT
    TATTCAAAGA AACAACATAA AACCAGCTGT GAAGCTGGTC TCTTGCATGG AGAGGAGGAT
    TATCTCAAAC GGCATAAGTC TTTGCAAATG GAGGGGCAGA AGCTCATTAA TCAGAGTTTA
    GTCATGGGGA TTGAGGTGGG CAAGAGTAGT GCCACAAACC AAAACACTGA GAAGGTTTTA
    CCCCCAAGAT TAAACCTTTG CCCAAGGACC AGCTATTCCA GCTTATCCTC CCCAGGCACT
    TCCCCATCCG GCTCATCAGT AAGCTCCCAA GACAGTGCTT TTTCTCAGAT TTCTGAACAC
    TCTGTGTTTA CACCCACTGA GACTTCCTCT CCAATAGATT GCACTTTTCA GGCTCAGAGA
    AAACGGGAAG ACCTTTCTCC TGACTTTAGC AATGCCAGCC ATGTTTCCGG AATGCCCGGT
    CCCTCATCAG GGCAGGCTTG CAGCCGCCCA GCCTATACAA AGAAGGACAC CATGGAGTGG
    CATTCACAAA TGCATTCTGT AACTCTTCAT CCCAGCACAT GGTTGAGAAA TGGTGTGGCC
    AGTTTGAAAA ACTGGTCCCT CAAAAAGAAA GCAAAGGCAG CCAGACCAGA GGAAGAGAAA
    ATAGCTTCTC CAAAAGGACC CTTAGAGCCA CCCCCACATG CTTCTGGTGT TCCAGAAGCC
    AACTCACTGC AAGAGGAACA AAAAGACTTG CCCTTAAGGG CAGCTGAAGG ACTGTCCCCT
    GTGCAGTCAG CCCAAAGGTG TAGTTCTTCT CCCTTCCAGG ACTCAGAGAG ACACTGTAGC
    TCTCCATTCA GCCTGGTGGA GAGCAGACTT AAGCTGTGCA TGAAGTCACA TGAGGAAATA
    GAGCCTGGTA GTCAGAGCTC TTCTGGTTCT CTGCCTTGGG AAAGAGCCTC AGCCAGCTCT
    TGGACTCTAG AGGATGCGAC CAGCCCAGAC TCAGGGCCTA CAGTGGTCTG CGACATTGAG
    GACAGGTATT TAACCAAAGA CATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12330
    Clone ID Related Accession (Same CDS sequence) NM_001258415.2 , NM_001258415.1
    Accession Version NM_001258415.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-24
    Organism Homo sapiens(human)
    Product rho GTPase-activating protein 20 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY496266.1 and AP001883.6. Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY496266.1, SRR1803616.252833.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCTGCCC GGGAGCGGCA GCCGGCGCTG AAGAAGAAAA TGAAAACACT AGCAGAAAGG 
    AGGAGGAGCG CTCCATCTCT TATCCTGGAT AAAGCCCTAC AAAAACGGCC TACTACCAGG
    GACAGTCCTT CTGCTAGTGT TGACACATGC ACATTTCTGT CATCATTAGT GTGCTCCAAT
    AGGACTCTGC TGATTGATGG CCGGGCAGAA CTCAAAAGAG GCCTCCAGAG GCAGGAGCGG
    CATCTTTTCC TATTCAATGA TCTGTTTGTT GTGGCCAAAA TCAAATATAA CAATAACTTT
    AAGATAAAAA ATAAAATTAA ATTAACTGAT ATGTGGACAG CAAGCTGTGT GGATGAAGTG
    GGAGAAGGCA ACACCAATGC CATGAAATCC TTTGTTTTGG GCTGGCCCAC AGTGAACTTT
    GTGGCCACTT TCAGTTCTCC AGAACAAAAG GACAAATGGC TCTCTCTCCT TCAGAGATAC
    ATCAATCTAG AGAAAGAAAA GGACTACCCG AAGAGCATTC CCCTCAAAAT CTTCGCCAAG
    GACATTGGGA ATTGTGCCTA CTCTAAAACT ATAACAGTAA TGAATTCAGA TACAGCGAAT
    GAAGTTATCA ACATGTCATT ACCAATGCTA GGGATAACTG GCTCTGAGAG AGATTACCAG
    TTGTGGGTCA ATTCTGGCAA AGAAGAGGCT CCATACCCAC TCATTGGGCA TGAATATCCA
    TATGGAATTA AAATGAGCCA TCTTCGAGAC TCTGCACTCC TGACACCGGG ATCAAAGGAC
    TCTACCACCC CTTTCAACCT CCAGGAGCCC TTCCTTATGG AACAGCTCCC CCGAGAGATG
    CAGTGCCAGT TCATCCTGAA GCCCAGCCGC CTGGCTGCAG CCCAGCAACT GAGTGATTCA
    GGTCATAAGA CATTTAAAAG GAGAAGATCT ATCATAAACT GGGCCTTCTG GCGAGGTTCT
    AGCACTCACC TGGACAACTT GCCCTCATCG CCAACATCAC CTATGCCAGG ACAGCTCTTT
    GGAATTTCTC TGCCAAATAT TTGTGAGAAT GACAATCTGC CCAAACCTGT CTTGGATATG
    CTTTTCTTTC TTAATCAAAA AGGACCTCTC ACCAAAGGTA TCTTCAGGCA ATCAGCCAAT
    GTGAAATCCT GCAGAGAACT AAAAGAGAAA TTGAATTCTG GAGTCGAAGT ACACCTAGAC
    TGTGAATCTA TTTTTGTGAT AGCATCTGTC TTAAAGGATT TTCTGCGAAA TATTCCAGGA
    AGTATTTTTT CATCAGATCT CTATGATCAC TGGGTCTCTG TAATGGATCA AGGAAATGAT
    GAAGAGAAAA TAAATACTGT TCAAAGGCTA TTAGACCAGC TTCCGAGAGC CAATGTTGTT
    CTCCTAAGGT ATCTTTTTGG GGTGTTACAC AACATTGAGC AACATTCCTC ATCCAATCAG
    ATGACTGCAT TTAATTTAGC TGTGTGTGTC GCTCCAAGTA TTCTTTGGCC TCCTGCTTCC
    TCCAGCCCAG AACTAGAAAA CGAATTTACA AAAAAGGTTT CCCTGCTTAT ACAATTTCTG
    ATTGAGAATT GCCTTAGGAT ATTTGGAGAA GAAATCACTT CCCTCTTCAG AGAGGTTTCA
    GTGAGATGTG ACACTAGAGA GAATGCCTCA GATATTTCTT GCTTTCAACT GAATGACTCC
    TCCTATGACA GCTTGGAAAA TGAGCTAAAT GAGGATGTTG ATGCACCATG CAGTGACTTG
    GTAAAGAAAC TTGGCCAGGG GAGCAGAAGC ATGGACTCTG TCTTAACCCT CAGTGACTAT
    GATCTTGACC AGCCCGAGGT GGAAGGCCTT TTAACCCTAA GCGACTTTGA CTTGGCCCAT
    TCTAAAGATG AAGATGTTCA AATGAAACGG CCTCTTGAAT CCAAGCCGGT GAACATTTTA
    GTGTACACAA AGATCCCACT GCGGGATCAT GCCAGGGCCC CATCTGCCAT GTGCACACCC
    AGCTACCTGT CCACAGCTGC AGCAAATGCT GCAAAAAGCC TGAGGCGACA CCGGCGTTGC
    TCAGAGCCCA GCATCGACTA TCTGGATTCA AAGCTTTCCT ACCTCAGGGA GTTTTATCAG
    AAAAAGCTAC GCAAGTCCAG CTGTGATGCA ATTCTTTCTC AAAAAGATGA AGACTATCTG
    AAGCAGAATC AACCCCTCCA GGAGGAAGGA AAGACATGTT TTAAACAGAG TTTAGTCACA
    GGCACTGATG TCAGCAAGAA AAATGCCACT ACTCAAAACA CTAAGAAGAA AAGCTTGTCT
    GGTAGTGAAG GAAATCACGT GAAACTTTTC CCTAAGTCTA AGCCAGTGGC CATTTCTGTG
    GCATCTTATA GTCCTATGTC CTCACAGGAT CATTCCAAGA ACCAGCCCTT TGATGTGAAT
    ACATCTGGAT ACTCCCCACC ACACACAGCA GATGCCCTCA AGGGTCCAAG GACACATCGG
    CGCTGCTCAG AGCCCAACAT AGAAGACCAG AACCGCAAGC TGACCTATCT CAGGGGAATT
    TATTCAAAGA AACAACATAA AACCAGCTGT GAAGCTGGTC TCTTGCATGG AGAGGAGGAT
    TATCTCAAAC GGCATAAGTC TTTGCAAATG GAGGGGCAGA AGCTCATTAA TCAGAGTTTA
    GTCATGGGGA TTGAGGTGGG CAAGAGTAGT GCCACAAACC AAAACACTGA GAAGGTTTTA
    CCCCCAAGAT TAAACCTTTG CCCAAGGACC AGCTATTCCA GCTTATCCTC CCCAGGCACT
    TCCCCATCCG GCTCATCAGT AAGCTCCCAA GACAGTGCTT TTTCTCAGAT TTCTGAACAC
    TCTGTGTTTA CACCCACTGA GACTTCCTCT CCAATAGATT GCACTTTTCA GGCTCAGAGA
    AAACGGGAAG ACCTTTCTCC TGACTTTAGC AATGCCAGCC ATGTTTCCGG AATGCCCGGT
    CCCTCATCAG GGCAGGCTTG CAGCCGCCCA GCCTATACAA AGAAGGACAC CATGGAGTGG
    CATTCACAAA TGCATTCTGT AACTCTTCAT CCCAGCACAT GGTTGAGAAA TGGTGTGGCC
    AGTTTGAAAA ACTGGTCCCT CAAAAAGAAA GCAAAGGCAG CCAGACCAGA GGAAGAGAAA
    ATAGCTTCTC CAAAAGGACC CTTAGAGCCA CCCCCACATG CTTCTGGTGT TCCAGAAGCC
    AACTCACTGC AAGAGGAACA AAAAGACTTG CCCTTAAGGG CAGCTGAAGG ACTGTCCCCT
    GTGCAGTCAG CCCAAAGGTG TAGTTCTTCT CCCTTCCAGG ACTCAGAGAG ACACTGTAGC
    TCTCCATTCA GCCTGGTGGA GAGCAGACTT AAGCTGTGCA TGAAGTCACA TGAGGAAATA
    GAGCCTGGTA GTCAGAGCTC TTCTGGTTCT CTGCCTTGGG AAAGAGCCTC AGCCAGCTCT
    TGGACTCTAG AGGATGCGAC CAGCCCAGAC TCAGGGCCTA CAGTGGTCTG CGACATTGAG
    GACAGGTATT TAACCAAAGA CATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245344.1
    CDS358..3864
    Translation

    Target ORF information:

    RefSeq Version NM_001258415.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258415.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCTGCCC GGGAGCGGCA GCCGGCGCTG AAGAAGAAAA TGAAAACACT AGCAGAAAGG 
    AGGAGGAGCG CTCCATCTCT TATCCTGGAT AAAGCCCTAC AAAAACGGCC TACTACCAGG
    GACAGTCCTT CTGCTAGTGT TGACACATGC ACATTTCTGT CATCATTAGT GTGCTCCAAT
    AGGACTCTGC TGATTGATGG CCGGGCAGAA CTCAAAAGAG GCCTCCAGAG GCAGGAGCGG
    CATCTTTTCC TATTCAATGA TCTGTTTGTT GTGGCCAAAA TCAAATATAA CAATAACTTT
    AAGATAAAAA ATAAAATTAA ATTAACTGAT ATGTGGACAG CAAGCTGTGT GGATGAAGTG
    GGAGAAGGCA ACACCAATGC CATGAAATCC TTTGTTTTGG GCTGGCCCAC AGTGAACTTT
    GTGGCCACTT TCAGTTCTCC AGAACAAAAG GACAAATGGC TCTCTCTCCT TCAGAGATAC
    ATCAATCTAG AGAAAGAAAA GGACTACCCG AAGAGCATTC CCCTCAAAAT CTTCGCCAAG
    GACATTGGGA ATTGTGCCTA CTCTAAAACT ATAACAGTAA TGAATTCAGA TACAGCGAAT
    GAAGTTATCA ACATGTCATT ACCAATGCTA GGGATAACTG GCTCTGAGAG AGATTACCAG
    TTGTGGGTCA ATTCTGGCAA AGAAGAGGCT CCATACCCAC TCATTGGGCA TGAATATCCA
    TATGGAATTA AAATGAGCCA TCTTCGAGAC TCTGCACTCC TGACACCGGG ATCAAAGGAC
    TCTACCACCC CTTTCAACCT CCAGGAGCCC TTCCTTATGG AACAGCTCCC CCGAGAGATG
    CAGTGCCAGT TCATCCTGAA GCCCAGCCGC CTGGCTGCAG CCCAGCAACT GAGTGATTCA
    GGTCATAAGA CATTTAAAAG GAGAAGATCT ATCATAAACT GGGCCTTCTG GCGAGGTTCT
    AGCACTCACC TGGACAACTT GCCCTCATCG CCAACATCAC CTATGCCAGG ACAGCTCTTT
    GGAATTTCTC TGCCAAATAT TTGTGAGAAT GACAATCTGC CCAAACCTGT CTTGGATATG
    CTTTTCTTTC TTAATCAAAA AGGACCTCTC ACCAAAGGTA TCTTCAGGCA ATCAGCCAAT
    GTGAAATCCT GCAGAGAACT AAAAGAGAAA TTGAATTCTG GAGTCGAAGT ACACCTAGAC
    TGTGAATCTA TTTTTGTGAT AGCATCTGTC TTAAAGGATT TTCTGCGAAA TATTCCAGGA
    AGTATTTTTT CATCAGATCT CTATGATCAC TGGGTCTCTG TAATGGATCA AGGAAATGAT
    GAAGAGAAAA TAAATACTGT TCAAAGGCTA TTAGACCAGC TTCCGAGAGC CAATGTTGTT
    CTCCTAAGGT ATCTTTTTGG GGTGTTACAC AACATTGAGC AACATTCCTC ATCCAATCAG
    ATGACTGCAT TTAATTTAGC TGTGTGTGTC GCTCCAAGTA TTCTTTGGCC TCCTGCTTCC
    TCCAGCCCAG AACTAGAAAA CGAATTTACA AAAAAGGTTT CCCTGCTTAT ACAATTTCTG
    ATTGAGAATT GCCTTAGGAT ATTTGGAGAA GAAATCACTT CCCTCTTCAG AGAGGTTTCA
    GTGAGATGTG ACACTAGAGA GAATGCCTCA GATATTTCTT GCTTTCAACT GAATGACTCC
    TCCTATGACA GCTTGGAAAA TGAGCTAAAT GAGGATGTTG ATGCACCATG CAGTGACTTG
    GTAAAGAAAC TTGGCCAGGG GAGCAGAAGC ATGGACTCTG TCTTAACCCT CAGTGACTAT
    GATCTTGACC AGCCCGAGGT GGAAGGCCTT TTAACCCTAA GCGACTTTGA CTTGGCCCAT
    TCTAAAGATG AAGATGTTCA AATGAAACGG CCTCTTGAAT CCAAGCCGGT GAACATTTTA
    GTGTACACAA AGATCCCACT GCGGGATCAT GCCAGGGCCC CATCTGCCAT GTGCACACCC
    AGCTACCTGT CCACAGCTGC AGCAAATGCT GCAAAAAGCC TGAGGCGACA CCGGCGTTGC
    TCAGAGCCCA GCATCGACTA TCTGGATTCA AAGCTTTCCT ACCTCAGGGA GTTTTATCAG
    AAAAAGCTAC GCAAGTCCAG CTGTGATGCA ATTCTTTCTC AAAAAGATGA AGACTATCTG
    AAGCAGAATC AACCCCTCCA GGAGGAAGGA AAGACATGTT TTAAACAGAG TTTAGTCACA
    GGCACTGATG TCAGCAAGAA AAATGCCACT ACTCAAAACA CTAAGAAGAA AAGCTTGTCT
    GGTAGTGAAG GAAATCACGT GAAACTTTTC CCTAAGTCTA AGCCAGTGGC CATTTCTGTG
    GCATCTTATA GTCCTATGTC CTCACAGGAT CATTCCAAGA ACCAGCCCTT TGATGTGAAT
    ACATCTGGAT ACTCCCCACC ACACACAGCA GATGCCCTCA AGGGTCCAAG GACACATCGG
    CGCTGCTCAG AGCCCAACAT AGAAGACCAG AACCGCAAGC TGACCTATCT CAGGGGAATT
    TATTCAAAGA AACAACATAA AACCAGCTGT GAAGCTGGTC TCTTGCATGG AGAGGAGGAT
    TATCTCAAAC GGCATAAGTC TTTGCAAATG GAGGGGCAGA AGCTCATTAA TCAGAGTTTA
    GTCATGGGGA TTGAGGTGGG CAAGAGTAGT GCCACAAACC AAAACACTGA GAAGGTTTTA
    CCCCCAAGAT TAAACCTTTG CCCAAGGACC AGCTATTCCA GCTTATCCTC CCCAGGCACT
    TCCCCATCCG GCTCATCAGT AAGCTCCCAA GACAGTGCTT TTTCTCAGAT TTCTGAACAC
    TCTGTGTTTA CACCCACTGA GACTTCCTCT CCAATAGATT GCACTTTTCA GGCTCAGAGA
    AAACGGGAAG ACCTTTCTCC TGACTTTAGC AATGCCAGCC ATGTTTCCGG AATGCCCGGT
    CCCTCATCAG GGCAGGCTTG CAGCCGCCCA GCCTATACAA AGAAGGACAC CATGGAGTGG
    CATTCACAAA TGCATTCTGT AACTCTTCAT CCCAGCACAT GGTTGAGAAA TGGTGTGGCC
    AGTTTGAAAA ACTGGTCCCT CAAAAAGAAA GCAAAGGCAG CCAGACCAGA GGAAGAGAAA
    ATAGCTTCTC CAAAAGGACC CTTAGAGCCA CCCCCACATG CTTCTGGTGT TCCAGAAGCC
    AACTCACTGC AAGAGGAACA AAAAGACTTG CCCTTAAGGG CAGCTGAAGG ACTGTCCCCT
    GTGCAGTCAG CCCAAAGGTG TAGTTCTTCT CCCTTCCAGG ACTCAGAGAG ACACTGTAGC
    TCTCCATTCA GCCTGGTGGA GAGCAGACTT AAGCTGTGCA TGAAGTCACA TGAGGAAATA
    GAGCCTGGTA GTCAGAGCTC TTCTGGTTCT CTGCCTTGGG AAAGAGCCTC AGCCAGCTCT
    TGGACTCTAG AGGATGCGAC CAGCCCAGAC TCAGGGCCTA CAGTGGTCTG CGACATTGAG
    GACAGGTATT TAACCAAAGA CATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08682
    Clone ID Related Accession (Same CDS sequence) XM_006718893.3 , NM_020809.3
    Accession Version XM_006718893.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rho GTPase-activating protein 20 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718893.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAGCGA TGTCCCCCCA GCAGGAGACT CTAGGGGGAC AGCCGGGGCG CTCCTCTTCC 
    CTGACAGGAG TGTCTCGGCT CGCGGGAGGC AGCTGCACCA AGAAGAAAAT GAAAACACTA
    GCAGAAAGGA GGAGGAGCGC TCCATCTCTT ATCCTGGATA AAGCCCTACA AAAACGGCCT
    ACTACCAGGG ACAGTCCTTC TGCTAGTGTT GACACATGCA CATTTCTGTC ATCATTAGTG
    TGCTCCAATA GGACTCTGCT GATTGATGGC CGGGCAGAAC TCAAAAGAGG CCTCCAGAGG
    CAGGAGCGGC ATCTTTTCCT ATTCAATGAT CTGTTTGTTG TGGCCAAAAT CAAATATAAC
    AATAACTTTA AGATAAAAAA TAAAATTAAA TTAACTGATA TGTGGACAGC AAGCTGTGTG
    GATGAAGTGG GAGAAGGCAA CACCAATGCC ATGAAATCCT TTGTTTTGGG CTGGCCCACA
    GTGAACTTTG TGGCCACTTT CAGTTCTCCA GAACAAAAGG ACAAATGGCT CTCTCTCCTT
    CAGAGATACA TCAATCTAGA GAAAGAAAAG GACTACCCGA AGAGCATTCC CCTCAAAATC
    TTCGCCAAGG ACATTGGGAA TTGTGCCTAC TCTAAAACTA TAACAGTAAT GAATTCAGAT
    ACAGCGAATG AAGTTATCAA CATGTCATTA CCAATGCTAG GGATAACTGG CTCTGAGAGA
    GATTACCAGT TGTGGGTCAA TTCTGGCAAA GAAGAGGCTC CATACCCACT CATTGGGCAT
    GAATATCCAT ATGGAATTAA AATGAGCCAT CTTCGAGACT CTGCACTCCT GACACCGGGA
    TCAAAGGACT CTACCACCCC TTTCAACCTC CAGGAGCCCT TCCTTATGGA ACAGCTCCCC
    CGAGAGATGC AGTGCCAGTT CATCCTGAAG CCCAGCCGCC TGGCTGCAGC CCAGCAACTG
    AGTGATTCAG GTCATAAGAC ATTTAAAAGG AGAAGATCTA TCATAAACTG GGCCTTCTGG
    CGAGGTTCTA GCACTCACCT GGACAACTTG CCCTCATCGC CAACATCACC TATGCCAGGA
    CAGCTCTTTG GAATTTCTCT GCCAAATATT TGTGAGAATG ACAATCTGCC CAAACCTGTC
    TTGGATATGC TTTTCTTTCT TAATCAAAAA GGACCTCTCA CCAAAGGTAT CTTCAGGCAA
    TCAGCCAATG TGAAATCCTG CAGAGAACTA AAAGAGAAAT TGAATTCTGG AGTCGAAGTA
    CACCTAGACT GTGAATCTAT TTTTGTGATA GCATCTGTCT TAAAGGATTT TCTGCGAAAT
    ATTCCAGGAA GTATTTTTTC ATCAGATCTC TATGATCACT GGGTCTCTGT AATGGATCAA
    GGAAATGATG AAGAGAAAAT AAATACTGTT CAAAGGCTAT TAGACCAGCT TCCGAGAGCC
    AATGTTGTTC TCCTAAGGTA TCTTTTTGGG GTGTTACACA ACATTGAGCA ACATTCCTCA
    TCCAATCAGA TGACTGCATT TAATTTAGCT GTGTGTGTCG CTCCAAGTAT TCTTTGGCCT
    CCTGCTTCCT CCAGCCCAGA ACTAGAAAAC GAATTTACAA AAAAGGTTTC CCTGCTTATA
    CAATTTCTGA TTGAGAATTG CCTTAGGATA TTTGGAGAAG AAATCACTTC CCTCTTCAGA
    GAGGTTTCAG TGAGATGTGA CACTAGAGAG AATGCCTCAG ATATTTCTTG CTTTCAACTG
    AATGACTCCT CCTATGACAG CTTGGAAAAT GAGCTAAATG AGGATGTTGA TGCACCATGC
    AGTGACTTGG TAAAGAAACT TGGCCAGGGG AGCAGAAGCA TGGACTCTGT CTTAACCCTC
    AGTGACTATG ATCTTGACCA GCCCGAGGTG GAAGGCCTTT TAACCCTAAG CGACTTTGAC
    TTGGCCCATT CTAAAGATGA AGATGTTCAA ATGAAACGGC CTCTTGAATC CAAGCCGGTG
    AACATTTTAG TGTACACAAA GATCCCACTG CGGGATCATG CCAGGGCCCC ATCTGCCATG
    TGCACACCCA GCTACCTGTC CACAGCTGCA GCAAATGCTG CAAAAAGCCT GAGGCGACAC
    CGGCGTTGCT CAGAGCCCAG CATCGACTAT CTGGATTCAA AGCTTTCCTA CCTCAGGGAG
    TTTTATCAGA AAAAGCTACG CAAGTCCAGC TGTGATGCAA TTCTTTCTCA AAAAGATGAA
    GACTATCTGA AGCAGAATCA ACCCCTCCAG GAGGAAGGAA AGACATGTTT TAAACAGAGT
    TTAGTCACAG GCACTGATGT CAGCAAGAAA AATGCCACTA CTCAAAACAC TAAGAAGAAA
    AGCTTGTCTG GTAGTGAAGG AAATCACGTG AAACTTTTCC CTAAGTCTAA GCCAGTGGCC
    ATTTCTGTGG CATCTTATAG TCCTATGTCC TCACAGGATC ATTCCAAGAA CCAGCCCTTT
    GATGTGAATA CATCTGGATA CTCCCCACCA CACACAGCAG ATGCCCTCAA GGGTCCAAGG
    ACACATCGGC GCTGCTCAGA GCCCAACATA GAAGACCAGA ACCGCAAGCT GACCTATCTC
    AGGGGAATTT ATTCAAAGAA ACAACATAAA ACCAGCTGTG AAGCTGGTCT CTTGCATGGA
    GAGGAGGATT ATCTCAAACG GCATAAGTCT TTGCAAATGG AGGGGCAGAA GCTCATTAAT
    CAGAGTTTAG TCATGGGGAT TGAGGTGGGC AAGAGTAGTG CCACAAACCA AAACACTGAG
    AAGGTTTTAC CCCCAAGATT AAACCTTTGC CCAAGGACCA GCTATTCCAG CTTATCCTCC
    CCAGGCACTT CCCCATCCGG CTCATCAGTA AGCTCCCAAG ACAGTGCTTT TTCTCAGATT
    TCTGAACACT CTGTGTTTAC ACCCACTGAG ACTTCCTCTC CAATAGATTG CACTTTTCAG
    GCTCAGAGAA AACGGGAAGA CCTTTCTCCT GACTTTAGCA ATGCCAGCCA TGTTTCCGGA
    ATGCCCGGTC CCTCATCAGG GCAGGCTTGC AGCCGCCCAG CCTATACAAA GAAGGACACC
    ATGGAGTGGC ATTCACAAAT GCATTCTGTA ACTCTTCATC CCAGCACATG GTTGAGAAAT
    GGTGTGGCCA GTTTGAAAAA CTGGTCCCTC AAAAAGAAAG CAAAGGCAGC CAGACCAGAG
    GAAGAGAAAA TAGCTTCTCC AAAAGGACCC TTAGAGCCAC CCCCACATGC TTCTGGTGTT
    CCAGAAGCCA ACTCACTGCA AGAGGAACAA AAAGACTTGC CCTTAAGGGC AGCTGAAGGA
    CTGTCCCCTG TGCAGTCAGC CCAAAGGTGT AGTTCTTCTC CCTTCCAGGA CTCAGAGAGA
    CACTGTAGCT CTCCATTCAG CCTGGTGGAG AGCAGACTTA AGCTGTGCAT GAAGTCACAT
    GAGGAAATAG AGCCTGGTAG TCAGAGCTCT TCTGGTTCTC TGCCTTGGGA AAGAGCCTCA
    GCCAGCTCTT GGACTCTAGA GGATGCGACC AGCCCAGACT CAGGGCCTAC AGTGGTCTGC
    GACATTGAGG ACAGGTATTT AACCAAAGAC ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718956.1
    CDS195..3770
    Translation

    Target ORF information:

    RefSeq Version XM_006718893.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718893.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAGCGA TGTCCCCCCA GCAGGAGACT CTAGGGGGAC AGCCGGGGCG CTCCTCTTCC 
    CTGACAGGAG TGTCTCGGCT CGCGGGAGGC AGCTGCACCA AGAAGAAAAT GAAAACACTA
    GCAGAAAGGA GGAGGAGCGC TCCATCTCTT ATCCTGGATA AAGCCCTACA AAAACGGCCT
    ACTACCAGGG ACAGTCCTTC TGCTAGTGTT GACACATGCA CATTTCTGTC ATCATTAGTG
    TGCTCCAATA GGACTCTGCT GATTGATGGC CGGGCAGAAC TCAAAAGAGG CCTCCAGAGG
    CAGGAGCGGC ATCTTTTCCT ATTCAATGAT CTGTTTGTTG TGGCCAAAAT CAAATATAAC
    AATAACTTTA AGATAAAAAA TAAAATTAAA TTAACTGATA TGTGGACAGC AAGCTGTGTG
    GATGAAGTGG GAGAAGGCAA CACCAATGCC ATGAAATCCT TTGTTTTGGG CTGGCCCACA
    GTGAACTTTG TGGCCACTTT CAGTTCTCCA GAACAAAAGG ACAAATGGCT CTCTCTCCTT
    CAGAGATACA TCAATCTAGA GAAAGAAAAG GACTACCCGA AGAGCATTCC CCTCAAAATC
    TTCGCCAAGG ACATTGGGAA TTGTGCCTAC TCTAAAACTA TAACAGTAAT GAATTCAGAT
    ACAGCGAATG AAGTTATCAA CATGTCATTA CCAATGCTAG GGATAACTGG CTCTGAGAGA
    GATTACCAGT TGTGGGTCAA TTCTGGCAAA GAAGAGGCTC CATACCCACT CATTGGGCAT
    GAATATCCAT ATGGAATTAA AATGAGCCAT CTTCGAGACT CTGCACTCCT GACACCGGGA
    TCAAAGGACT CTACCACCCC TTTCAACCTC CAGGAGCCCT TCCTTATGGA ACAGCTCCCC
    CGAGAGATGC AGTGCCAGTT CATCCTGAAG CCCAGCCGCC TGGCTGCAGC CCAGCAACTG
    AGTGATTCAG GTCATAAGAC ATTTAAAAGG AGAAGATCTA TCATAAACTG GGCCTTCTGG
    CGAGGTTCTA GCACTCACCT GGACAACTTG CCCTCATCGC CAACATCACC TATGCCAGGA
    CAGCTCTTTG GAATTTCTCT GCCAAATATT TGTGAGAATG ACAATCTGCC CAAACCTGTC
    TTGGATATGC TTTTCTTTCT TAATCAAAAA GGACCTCTCA CCAAAGGTAT CTTCAGGCAA
    TCAGCCAATG TGAAATCCTG CAGAGAACTA AAAGAGAAAT TGAATTCTGG AGTCGAAGTA
    CACCTAGACT GTGAATCTAT TTTTGTGATA GCATCTGTCT TAAAGGATTT TCTGCGAAAT
    ATTCCAGGAA GTATTTTTTC ATCAGATCTC TATGATCACT GGGTCTCTGT AATGGATCAA
    GGAAATGATG AAGAGAAAAT AAATACTGTT CAAAGGCTAT TAGACCAGCT TCCGAGAGCC
    AATGTTGTTC TCCTAAGGTA TCTTTTTGGG GTGTTACACA ACATTGAGCA ACATTCCTCA
    TCCAATCAGA TGACTGCATT TAATTTAGCT GTGTGTGTCG CTCCAAGTAT TCTTTGGCCT
    CCTGCTTCCT CCAGCCCAGA ACTAGAAAAC GAATTTACAA AAAAGGTTTC CCTGCTTATA
    CAATTTCTGA TTGAGAATTG CCTTAGGATA TTTGGAGAAG AAATCACTTC CCTCTTCAGA
    GAGGTTTCAG TGAGATGTGA CACTAGAGAG AATGCCTCAG ATATTTCTTG CTTTCAACTG
    AATGACTCCT CCTATGACAG CTTGGAAAAT GAGCTAAATG AGGATGTTGA TGCACCATGC
    AGTGACTTGG TAAAGAAACT TGGCCAGGGG AGCAGAAGCA TGGACTCTGT CTTAACCCTC
    AGTGACTATG ATCTTGACCA GCCCGAGGTG GAAGGCCTTT TAACCCTAAG CGACTTTGAC
    TTGGCCCATT CTAAAGATGA AGATGTTCAA ATGAAACGGC CTCTTGAATC CAAGCCGGTG
    AACATTTTAG TGTACACAAA GATCCCACTG CGGGATCATG CCAGGGCCCC ATCTGCCATG
    TGCACACCCA GCTACCTGTC CACAGCTGCA GCAAATGCTG CAAAAAGCCT GAGGCGACAC
    CGGCGTTGCT CAGAGCCCAG CATCGACTAT CTGGATTCAA AGCTTTCCTA CCTCAGGGAG
    TTTTATCAGA AAAAGCTACG CAAGTCCAGC TGTGATGCAA TTCTTTCTCA AAAAGATGAA
    GACTATCTGA AGCAGAATCA ACCCCTCCAG GAGGAAGGAA AGACATGTTT TAAACAGAGT
    TTAGTCACAG GCACTGATGT CAGCAAGAAA AATGCCACTA CTCAAAACAC TAAGAAGAAA
    AGCTTGTCTG GTAGTGAAGG AAATCACGTG AAACTTTTCC CTAAGTCTAA GCCAGTGGCC
    ATTTCTGTGG CATCTTATAG TCCTATGTCC TCACAGGATC ATTCCAAGAA CCAGCCCTTT
    GATGTGAATA CATCTGGATA CTCCCCACCA CACACAGCAG ATGCCCTCAA GGGTCCAAGG
    ACACATCGGC GCTGCTCAGA GCCCAACATA GAAGACCAGA ACCGCAAGCT GACCTATCTC
    AGGGGAATTT ATTCAAAGAA ACAACATAAA ACCAGCTGTG AAGCTGGTCT CTTGCATGGA
    GAGGAGGATT ATCTCAAACG GCATAAGTCT TTGCAAATGG AGGGGCAGAA GCTCATTAAT
    CAGAGTTTAG TCATGGGGAT TGAGGTGGGC AAGAGTAGTG CCACAAACCA AAACACTGAG
    AAGGTTTTAC CCCCAAGATT AAACCTTTGC CCAAGGACCA GCTATTCCAG CTTATCCTCC
    CCAGGCACTT CCCCATCCGG CTCATCAGTA AGCTCCCAAG ACAGTGCTTT TTCTCAGATT
    TCTGAACACT CTGTGTTTAC ACCCACTGAG ACTTCCTCTC CAATAGATTG CACTTTTCAG
    GCTCAGAGAA AACGGGAAGA CCTTTCTCCT GACTTTAGCA ATGCCAGCCA TGTTTCCGGA
    ATGCCCGGTC CCTCATCAGG GCAGGCTTGC AGCCGCCCAG CCTATACAAA GAAGGACACC
    ATGGAGTGGC ATTCACAAAT GCATTCTGTA ACTCTTCATC CCAGCACATG GTTGAGAAAT
    GGTGTGGCCA GTTTGAAAAA CTGGTCCCTC AAAAAGAAAG CAAAGGCAGC CAGACCAGAG
    GAAGAGAAAA TAGCTTCTCC AAAAGGACCC TTAGAGCCAC CCCCACATGC TTCTGGTGTT
    CCAGAAGCCA ACTCACTGCA AGAGGAACAA AAAGACTTGC CCTTAAGGGC AGCTGAAGGA
    CTGTCCCCTG TGCAGTCAGC CCAAAGGTGT AGTTCTTCTC CCTTCCAGGA CTCAGAGAGA
    CACTGTAGCT CTCCATTCAG CCTGGTGGAG AGCAGACTTA AGCTGTGCAT GAAGTCACAT
    GAGGAAATAG AGCCTGGTAG TCAGAGCTCT TCTGGTTCTC TGCCTTGGGA AAGAGCCTCA
    GCCAGCTCTT GGACTCTAGA GGATGCGACC AGCCCAGACT CAGGGCCTAC AGTGGTCTGC
    GACATTGAGG ACAGGTATTT AACCAAAGAC ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.