Arhgap20 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arhgap20
Entrez Gene ID	244867
Full Name	Rho GTPase activating protein 20
Synonyms	6530403F17Rik,A530023E23Rik,RARhoGAP,mKIAA1391
General protein information	Preferred Names Rho GTPase activating protein 20 Names rho GTPase-activating protein 20 RA and RhoGAP domain-containing protein rho-type GTPase-activating protein 20
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A5.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_175535.3	NP_780744.2	rho GTPase-activating protein 20
NM_175535.4	NP_780744.2	rho GTPase-activating protein 20

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARHGAP20	XP_003313436.1
Mus musculus (house mouse)	Arhgap20	NP_780744.2
Gallus gallus (chicken)	ARHGAP20	XP_417154.2
Homo sapiens (human)	ARHGAP20	NP_065860.2
Bos taurus (cattle)	ARHGAP20	NP_001193662.1
	ARHGAP20	XP_001098860.2
Canis lupus familiaris (dog)	ARHGAP20	XP_005619862.1
Rattus norvegicus (Norway rat)	Arhgap20	NP_998794.1

Gene Ontology
Bibliography

Related articles in PubMed

cAMP-Epac2-mediated activation of Rap1 in developing male germ cells: RA-RhoGAP as a possible direct down-stream effector.
Aivatiadou E, Ripolone M, Brunetti F, Berruti G
Molecular reproduction and development76(4)407-16(2009 Apr)

A novel group of multi-GAP-domain proteins.
Ruan Y, Sensen CW, van der Hoorn FA
Molecular reproduction and development75(10)1578-89(2008 Oct)

Ovarian gene expression in the absence of FIGLA, an oocyte-specific transcription factor.
Joshi S, Davies H, Sims LP, Levy SE, Dean J
BMC developmental biology767(2007 Jun)

Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2.
Zhao Y, Ransom JF, Li A, Vedantham V, von Drehle M, Muth AN, Tsuchihashi T, McManus MT, Schwartz RJ, Srivastava D
Cell129(2)303-17(2007 Apr)

Characterization of the piRNA complex from rat testes.
Lau NC, Seto AG, Kim J, Kuramochi-Miyagawa S, Nakano T, Bartel DP, Kingston RE
Science (New York, N.Y.)313(5785)363-7(2006 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results are consistent with a cAMP/Epac2/Rap1-mediated signaling that could exert its action, among others, through RA-RhoGAP to promote the progression of spermatogenesis.
Title: cAMP-Epac2-mediated activation of Rap1 in developing male germ cells: RA-RhoGAP as a possible direct down-stream effector.

human KIAA1391 gene and mouse Kiaa1391 gene encode RhoGAP proteins with RA domain and two ANXL repeats
Title: Identification and characterization of human KIAA1391 and mouse Kiaa1391 genes encoding novel RhoGAP family proteins with RA domain and ANXL repeats.

The following Arhgap20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arhgap20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05533	NM_175535.4 Latest version!	Mus musculus Rho GTPase activating protein 20 (Arhgap20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu05533	NM_175535.3	Mus musculus Rho GTPase activating protein 20 (Arhgap20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arhgap20 ( NM_175535.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780744.2 Mus musculus Rho GTPase activating protein 20 (Arhgap20), mRNA.

Price & Availability↑

CloneID	OMu05533
Clone ID Related Accession (Same CDS sequence)	NM_175535.3 , NM_175535.4
Accession Version	NM_175535.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 20
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BK004077.1. On May 24, 2004 this sequence version replaced NM_175535.2. ##Evidence-Data-START## Transcript exon combination :: BK004077.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN00849390 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGCGA TGTCTCCGCA GCAGGATGCT CTAGGGGCGC AGCCGGGGCG CTCCTCCTCG 
CTGACAGGCA TGTCTCGCAT CGCGGGAGGC CCTGGCACCA AGAAGAAAAT GAAAACACTC 
GCAGAAAGGA GGAGAAGCGC CCCGTCCCTC ATCCTGGACA AAGCCTTACA GAAGCGGCCG 
AGCACCAGAG ACAGCCATTC TGCCAGCATT GACACGTGTG CATTCCTCTC GTCCTTCATG 
TGCTCCAGTC GCACTCTGCT GATTGATGGT CCAGTGGAAC TTAAAAGAGG CCTGCAGAGG 
CAGGAGAGGC ATCTTTTCCT ATTCAACGAT CTGTTTGTCT CAGCCAAAAT CAAATATAAC 
AACAACTTTA AGATAAAGAA TAAAATAAGA CTAACTGACA TGTGGACAGC TAGCTGTGTG 
GAGGAAGTGG GAGAAGGCAA CATGAATGCT CAGAAGTCCT TTGTCCTGGG CTGGCCCACC 
GTGAACTTTG TGGCTACTTT CAGTTCTCCA GAACAAAAGG ACAAATGGCT GTCTCTCCTT 
CAGAGATACA TCGCTCTAGA AAAGGAGAAA GACTACCCGA AGAGCATCCC CCTCAAAATC 
TTTGCCAAGG ACATTGGAAA CTGTGCCTAT TTTAAAACTA TAACAGTGAT GAATTCAGAC 
ACAGCGAGTG AAGTTATCAA CATGTCACTG CAAATGTTAG GAATAACTGG CTCTGAGAGA 
GATTACCAAC TGTGGGTAAA TTCTGGAAAA GAAGCGGCAC CGTACCCACT CATTGGACAC 
GAGTATCCTT ATGGAATTAA AATGAGCCAC CTCCGAGACA CTGCCCTTCT GACGCAGGGA 
TCAAGAGACT CTGCCAGCCC ATCCCAGCTC CAAGAGCCTT TCCTCATGGA GCAGCTGCCC 
CGCGAGATGC AATGCCAGTT CATCCTGAAA CCTACCCGCC TGGCGACAGC CCAGCAACTG 
AGCGATTCCA GCCAGAAGAC ATTTAAAAGG AGGCGCTCTA TCATCAACTG GGCTTTTTGG 
CGGGGTTCTA GCACACACCT GGACAACCTG CCCATGTCAC CAACATCCCC TATGCCAGGA 
CAGCTCTTTG GAGTTTCTTT ACCAGATCTT TGTGAGAACG ACAATCTGCC AAAGCCTATC 
TTGGATATGC TTTCCTTCCT TAACCAAAAA GGCCCCCTCA CAAAAGGCAT CTTCAGGCAG 
TCGGCCAACA TGAAATCTTG CAGAGAGCTA AAGGAGAAAC TGAATTCTGG AATCGAAGTC 
CACCTTGACT GTGAATCCAT CTTTGTGATA GCATCTGTGT TGAAGGATTT TCTGAGAAAT 
ATCCCAGAAA GTATCTTTTC ATCAGATCTA TATGATCACT GGGTCTGTGT AATGGATCAA 
GGAAATGATG AAGAAAAAAT AAATATAATT CAAAGGCTAT TAGATCAGCT TCCCAGAGCC 
AATGTTGTTT TCCTAAGGTA TCTGTTTGGG GTATTACACA ACATTGAACA ACATTCCTTG 
TCTAATCAGA TGACTGCATT TAACTTAGCA GTGTGTATCG CTCCAAGCAT TCTTTGGCCT 
CCTGCTTCCT CCAGTCCAGA GCTAGAAAAT GAATTTACAA AAAAGGTTTC TCTTCTTATA 
CAATTTCTGA TTGAAAACTG CTGTAGGGTA TTTGGAGAAG AAATCGCTTC TCTCTTGGGG 
GAGCTTTCGG AGAGAAGCGA CAGAGAACAC ACCCCAGATA TAACTTGCTT CCAAATGAAC 
GACTCCTCCT ATGACAGCTT GGAAAACGAG CTCAATGAGG AAGCTGATGC TCCATGCAGT 
GACCTGGTCA AAAAGCTTGG CCAGGGTAGC AGGAGCATGG ACTCTGTCTT AACCCTCAGC 
GACTATGACC TTGAACAGCC TGAGGTGGAA GGCCTCTTAA CCCTGAGCAA CTTTGACTTA 
GATCAGTCTA AAGAGGAGCA CATTCCGATA AAGCCACCTC TGGAGCCCAA GCCAGTGAAT 
GTTTTCGTAG GCTACAGGAA GGTTTCACTA GGGGAGCACG CTAGGGCCCC AGCTGGCCCA 
GGCACACTCA GCTGCCTGCC TGTGGCTGCA GCAGATGCCC CGAAAGTCCT GCGGCGGCAC 
CGGCGCTCCT CAGAACCCAG CATTGACTAT CTAGACACAA AGCTTTCCTA TCTCCGGGAG 
TTTTACCAGA AAAAGCTGCG CAAGTCCAGC TGCGATGCTG TCCTCTCTAG AAAAGATGAA 
GATTACCTGA AGCAGACCCA GCCTCAGAAG AAAGGGGATA AGGTGTGTCT TAAACAGAGT 
TCAGTGACAG GCACTGACGT CAGCAAGAGA AACACAGCTA ATGAAAACAT TAAGAAGAAA 
AGCTTGTCTG GTCATGAAGG AACTCAAGTG ACACTTTTTA CTAAGTCCAA GCCAGTACCC 
ATCTCTGTGG CATCTTACAG CCACGGGTCC TCCCAGGATC ATCCCAGGAA GCAAGCCTTT 
GATGCAGACC CATGCCGGTT CTCCCCACCA CACCTAACAG ATGCCCAAAA GAGCTCAAGG 
GTGCAACATC GGCGCTGTTC AGAACCCAGC ATAGATGACC AGAACTACAA GCTGTCCTAT 
CTCAGGGGGA TCTATTCAAT GAAACAAAAT AAAGCCAGCT GTGAGGCTGG CCTCTTGCAT 
GGGGAGGACG ACTATCTCAG AAGGCATAAG TCTTTGCAAA TCGAGGGCCA AAAGCTTATT 
AATCAGAGTT TGGTCATGGG CATTGAGGTG GGCAAGAGCA GCAGCTCTCA CCAAAGCACT 
GAGAAGGTTT TGCCCCCAAG ACTAAACCTT TGCCCAAGGG CAAGCTATTC AAGCTTATCT 
TCTCCAGGCT CGTCCCCATC CGGCTCCTCT GTAAGCTCCC AGGACAGCGC TTTCTCTCAG 
ATCTCTGAAC ACTCCGTGTT TACGCCCACC GAAACCTCCT CACCAATAGA TTGCACTTTC 
CAGACACAAA GAAAACAAGA AGAGCTGTCT TCAGACTTTG ACAGTCCCAG CCGCCTTTCC 
GGAATGCCCG GTCCCTCCAT GGGTCAGGCC AGCAGCCACC TAGCTTATTT AAGAAAGGGT 
ACCACGGAGC AGCCATCACA AATGCATTCT GTAACCCTTC ACCCCAGTGC GTGGTTGAGA 
AGTGGCTTGG TCACTTTAAA AAACTGGTCC CTCAAAAAGA AAACAAAAGC AGCCAGACCA 
GAAGACAGGA AAGTCTGTTC CCTGAAAGAG CCTCTGGAGT TGCCGTCATG TGCTTCCGGT 
ACCCCAGAGG CTGACTCTCT ACAAGAGAGT CAGGATGACT TACAGGGAGA TGAAGGACCT 
GGGCAAACAG CCTGTGGATT CAGTTCTTAT GCCTGTCAGG ACTCAGAGCA ACATGCCGGC 
TCTCCATTCC ACCTGGCAGA AAGCAGACTC AAGCCTTGTA TGAAGTTATA TAAGGGAGAA 
GAGAGTGGAG GGCAGTACCC TTGTGATAAC CCTTGGGAAG GAGCCTCTTC AAGTCTGGAG 
ACCACGGAGG ATACAGCCAA CCCAGGTGCA GAACCTACAA CATTTGCGAT GACGGGGACA 
GATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780744.2
CDS	157..3705
Translation	MEAMSPQQDALGAQPGRSSSLTGMSRIAGGPGTKKKMKTLAERRRSAPSLILDKALQKRPSTRDSHSASIDTCAFLSSFMCSSRTLLIDGPVELKRGLQRQERHLFLFNDLFVSAKIKYNNNFKIKNKIRLTDMWTASCVEEVGEGNMNAQKSFVLGWPTVNFVATFSSPEQKDKWLSLLQRYIALEKEKDYPKSIPLKIFAKDIGNCAYFKTITVMNSDTASEVINMSLQMLGITGSERDYQLWVNSGKEAAPYPLIGHEYPYGIKMSHLRDTALLTQGSRDSASPSQLQEPFLMEQLPREMQCQFILKPTRLATAQQLSDSSQKTFKRRRSIINWAFWRGSSTHLDNLPMSPTSPMPGQLFGVSLPDLCENDNLPKPILDMLSFLNQKGPLTKGIFRQSANMKSCRELKEKLNSGIEVHLDCESIFVIASVLKDFLRNIPESIFSSDLYDHWVCVMDQGNDEEKINIIQRLLDQLPRANVVFLRYLFGVLHNIEQHSLSNQMTAFNLAVCIAPSILWPPASSSPELENEFTKKVSLLIQFLIENCCRVFGEEIASLLGELSERSDREHTPDITCFQMNDSSYDSLENELNEEADAPCSDLVKKLGQGSRSMDSVLTLSDYDLEQPEVEGLLTLSNFDLDQSKEEHIPIKPPLEPKPVNVFVGYRKVSLGEHARAPAGPGTLSCLPVAAADAPKVLRRHRRSSEPSIDYLDTKLSYLREFYQKKLRKSSCDAVLSRKDEDYLKQTQPQKKGDKVCLKQSSVTGTDVSKRNTANENIKKKSLSGHEGTQVTLFTKSKPVPISVASYSHGSSQDHPRKQAFDADPCRFSPPHLTDAQKSSRVQHRRCSEPSIDDQNYKLSYLRGIYSMKQNKASCEAGLLHGEDDYLRRHKSLQIEGQKLINQSLVMGIEVGKSSSSHQSTEKVLPPRLNLCPRASYSSLSSPGSSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQTQRKQEELSSDFDSPSRLSGMPGPSMGQASSHLAYLRKGTTEQPSQMHSVTLHPSAWLRSGLVTLKNWSLKKKTKAARPEDRKVCSLKEPLELPSCASGTPEADSLQESQDDLQGDEGPGQTACGFSSYACQDSEQHAGSPFHLAESRLKPCMKLYKGEESGGQYPCDNPWEGASSSLETTEDTANPGAEPTTFAMTGTDI

Target ORF information:

RefSeq Version	NM_175535.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 20 (Arhgap20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175535.3

ORF Insert Sequence:

ATGGAAGCGA TGTCTCCGCA GCAGGATGCT CTAGGGGCGC AGCCGGGGCG CTCCTCCTCG 
CTGACAGGCA TGTCTCGCAT CGCGGGAGGC CCTGGCACCA AGAAGAAAAT GAAAACACTC 
GCAGAAAGGA GGAGAAGCGC CCCGTCCCTC ATCCTGGACA AAGCCTTACA GAAGCGGCCG 
AGCACCAGAG ACAGCCATTC TGCCAGCATT GACACGTGTG CATTCCTCTC GTCCTTCATG 
TGCTCCAGTC GCACTCTGCT GATTGATGGT CCAGTGGAAC TTAAAAGAGG CCTGCAGAGG 
CAGGAGAGGC ATCTTTTCCT ATTCAACGAT CTGTTTGTCT CAGCCAAAAT CAAATATAAC 
AACAACTTTA AGATAAAGAA TAAAATAAGA CTAACTGACA TGTGGACAGC TAGCTGTGTG 
GAGGAAGTGG GAGAAGGCAA CATGAATGCT CAGAAGTCCT TTGTCCTGGG CTGGCCCACC 
GTGAACTTTG TGGCTACTTT CAGTTCTCCA GAACAAAAGG ACAAATGGCT GTCTCTCCTT 
CAGAGATACA TCGCTCTAGA AAAGGAGAAA GACTACCCGA AGAGCATCCC CCTCAAAATC 
TTTGCCAAGG ACATTGGAAA CTGTGCCTAT TTTAAAACTA TAACAGTGAT GAATTCAGAC 
ACAGCGAGTG AAGTTATCAA CATGTCACTG CAAATGTTAG GAATAACTGG CTCTGAGAGA 
GATTACCAAC TGTGGGTAAA TTCTGGAAAA GAAGCGGCAC CGTACCCACT CATTGGACAC 
GAGTATCCTT ATGGAATTAA AATGAGCCAC CTCCGAGACA CTGCCCTTCT GACGCAGGGA 
TCAAGAGACT CTGCCAGCCC ATCCCAGCTC CAAGAGCCTT TCCTCATGGA GCAGCTGCCC 
CGCGAGATGC AATGCCAGTT CATCCTGAAA CCTACCCGCC TGGCGACAGC CCAGCAACTG 
AGCGATTCCA GCCAGAAGAC ATTTAAAAGG AGGCGCTCTA TCATCAACTG GGCTTTTTGG 
CGGGGTTCTA GCACACACCT GGACAACCTG CCCATGTCAC CAACATCCCC TATGCCAGGA 
CAGCTCTTTG GAGTTTCTTT ACCAGATCTT TGTGAGAACG ACAATCTGCC AAAGCCTATC 
TTGGATATGC TTTCCTTCCT TAACCAAAAA GGCCCCCTCA CAAAAGGCAT CTTCAGGCAG 
TCGGCCAACA TGAAATCTTG CAGAGAGCTA AAGGAGAAAC TGAATTCTGG AATCGAAGTC 
CACCTTGACT GTGAATCCAT CTTTGTGATA GCATCTGTGT TGAAGGATTT TCTGAGAAAT 
ATCCCAGAAA GTATCTTTTC ATCAGATCTA TATGATCACT GGGTCTGTGT AATGGATCAA 
GGAAATGATG AAGAAAAAAT AAATATAATT CAAAGGCTAT TAGATCAGCT TCCCAGAGCC 
AATGTTGTTT TCCTAAGGTA TCTGTTTGGG GTATTACACA ACATTGAACA ACATTCCTTG 
TCTAATCAGA TGACTGCATT TAACTTAGCA GTGTGTATCG CTCCAAGCAT TCTTTGGCCT 
CCTGCTTCCT CCAGTCCAGA GCTAGAAAAT GAATTTACAA AAAAGGTTTC TCTTCTTATA 
CAATTTCTGA TTGAAAACTG CTGTAGGGTA TTTGGAGAAG AAATCGCTTC TCTCTTGGGG 
GAGCTTTCGG AGAGAAGCGA CAGAGAACAC ACCCCAGATA TAACTTGCTT CCAAATGAAC 
GACTCCTCCT ATGACAGCTT GGAAAACGAG CTCAATGAGG AAGCTGATGC TCCATGCAGT 
GACCTGGTCA AAAAGCTTGG CCAGGGTAGC AGGAGCATGG ACTCTGTCTT AACCCTCAGC 
GACTATGACC TTGAACAGCC TGAGGTGGAA GGCCTCTTAA CCCTGAGCAA CTTTGACTTA 
GATCAGTCTA AAGAGGAGCA CATTCCGATA AAGCCACCTC TGGAGCCCAA GCCAGTGAAT 
GTTTTCGTAG GCTACAGGAA GGTTTCACTA GGGGAGCACG CTAGGGCCCC AGCTGGCCCA 
GGCACACTCA GCTGCCTGCC TGTGGCTGCA GCAGATGCCC CGAAAGTCCT GCGGCGGCAC 
CGGCGCTCCT CAGAACCCAG CATTGACTAT CTAGACACAA AGCTTTCCTA TCTCCGGGAG 
TTTTACCAGA AAAAGCTGCG CAAGTCCAGC TGCGATGCTG TCCTCTCTAG AAAAGATGAA 
GATTACCTGA AGCAGACCCA GCCTCAGAAG AAAGGGGATA AGGTGTGTCT TAAACAGAGT 
TCAGTGACAG GCACTGACGT CAGCAAGAGA AACACAGCTA ATGAAAACAT TAAGAAGAAA 
AGCTTGTCTG GTCATGAAGG AACTCAAGTG ACACTTTTTA CTAAGTCCAA GCCAGTACCC 
ATCTCTGTGG CATCTTACAG CCACGGGTCC TCCCAGGATC ATCCCAGGAA GCAAGCCTTT 
GATGCAGACC CATGCCGGTT CTCCCCACCA CACCTAACAG ATGCCCAAAA GAGCTCAAGG 
GTGCAACATC GGCGCTGTTC AGAACCCAGC ATAGATGACC AGAACTACAA GCTGTCCTAT 
CTCAGGGGGA TCTATTCAAT GAAACAAAAT AAAGCCAGCT GTGAGGCTGG CCTCTTGCAT 
GGGGAGGACG ACTATCTCAG AAGGCATAAG TCTTTGCAAA TCGAGGGCCA AAAGCTTATT 
AATCAGAGTT TGGTCATGGG CATTGAGGTG GGCAAGAGCA GCAGCTCTCA CCAAAGCACT 
GAGAAGGTTT TGCCCCCAAG ACTAAACCTT TGCCCAAGGG CAAGCTATTC AAGCTTATCT 
TCTCCAGGCT CGTCCCCATC CGGCTCCTCT GTAAGCTCCC AGGACAGCGC TTTCTCTCAG 
ATCTCTGAAC ACTCCGTGTT TACGCCCACC GAAACCTCCT CACCAATAGA TTGCACTTTC 
CAGACACAAA GAAAACAAGA AGAGCTGTCT TCAGACTTTG ACAGTCCCAG CCGCCTTTCC 
GGAATGCCCG GTCCCTCCAT GGGTCAGGCC AGCAGCCACC TAGCTTATTT AAGAAAGGGT 
ACCACGGAGC AGCCATCACA AATGCATTCT GTAACCCTTC ACCCCAGTGC GTGGTTGAGA 
AGTGGCTTGG TCACTTTAAA AAACTGGTCC CTCAAAAAGA AAACAAAAGC AGCCAGACCA 
GAAGACAGGA AAGTCTGTTC CCTGAAAGAG CCTCTGGAGT TGCCGTCATG TGCTTCCGGT 
ACCCCAGAGG CTGACTCTCT ACAAGAGAGT CAGGATGACT TACAGGGAGA TGAAGGACCT 
GGGCAAACAG CCTGTGGATT CAGTTCTTAT GCCTGTCAGG ACTCAGAGCA ACATGCCGGC 
TCTCCATTCC ACCTGGCAGA AAGCAGACTC AAGCCTTGTA TGAAGTTATA TAAGGGAGAA 
GAGAGTGGAG GGCAGTACCC TTGTGATAAC CCTTGGGAAG GAGCCTCTTC AAGTCTGGAG 
ACCACGGAGG ATACAGCCAA CCCAGGTGCA GAACCTACAA CATTTGCGAT GACGGGGACA 
GATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap20 ( NM_175535.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780744.2 Mus musculus Rho GTPase activating protein 20 (Arhgap20), mRNA.

Price & Availability↑

CloneID	OMu05533
Clone ID Related Accession (Same CDS sequence)	NM_175535.3 , NM_175535.4
Accession Version	NM_175535.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 20
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC162806.4 and AC140363.3. On Aug 15, 2018 this sequence version replaced NM_175535.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BK004077.1, SRR7345562.2294737.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN00849390 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGCGA TGTCTCCGCA GCAGGATGCT CTAGGGGCGC AGCCGGGGCG CTCCTCCTCG 
CTGACAGGCA TGTCTCGCAT CGCGGGAGGC CCTGGCACCA AGAAGAAAAT GAAAACACTC 
GCAGAAAGGA GGAGAAGCGC CCCGTCCCTC ATCCTGGACA AAGCCTTACA GAAGCGGCCG 
AGCACCAGAG ACAGCCATTC TGCCAGCATT GACACGTGTG CATTCCTCTC GTCCTTCATG 
TGCTCCAGTC GCACTCTGCT GATTGATGGT CCAGTGGAAC TTAAAAGAGG CCTGCAGAGG 
CAGGAGAGGC ATCTTTTCCT ATTCAACGAT CTGTTTGTCT CAGCCAAAAT CAAATATAAC 
AACAACTTTA AGATAAAGAA TAAAATAAGA CTAACTGACA TGTGGACAGC TAGCTGTGTG 
GAGGAAGTGG GAGAAGGCAA CATGAATGCT CAGAAGTCCT TTGTCCTGGG CTGGCCCACC 
GTGAACTTTG TGGCTACTTT CAGTTCTCCA GAACAAAAGG ACAAATGGCT GTCTCTCCTT 
CAGAGATACA TCGCTCTAGA AAAGGAGAAA GACTACCCGA AGAGCATCCC CCTCAAAATC 
TTTGCCAAGG ACATTGGAAA CTGTGCCTAT TTTAAAACTA TAACAGTGAT GAATTCAGAC 
ACAGCGAGTG AAGTTATCAA CATGTCACTG CAAATGTTAG GAATAACTGG CTCTGAGAGA 
GATTACCAAC TGTGGGTAAA TTCTGGAAAA GAAGCGGCAC CGTACCCACT CATTGGACAC 
GAGTATCCTT ATGGAATTAA AATGAGCCAC CTCCGAGACA CTGCCCTTCT GACGCAGGGA 
TCAAGAGACT CTGCCAGCCC ATCCCAGCTC CAAGAGCCTT TCCTCATGGA GCAGCTGCCC 
CGCGAGATGC AATGCCAGTT CATCCTGAAA CCTACCCGCC TGGCGACAGC CCAGCAACTG 
AGCGATTCCA GCCAGAAGAC ATTTAAAAGG AGGCGCTCTA TCATCAACTG GGCTTTTTGG 
CGGGGTTCTA GCACACACCT GGACAACCTG CCCATGTCAC CAACATCCCC TATGCCAGGA 
CAGCTCTTTG GAGTTTCTTT ACCAGATCTT TGTGAGAACG ACAATCTGCC AAAGCCTATC 
TTGGATATGC TTTCCTTCCT TAACCAAAAA GGCCCCCTCA CAAAAGGCAT CTTCAGGCAG 
TCGGCCAACA TGAAATCTTG CAGAGAGCTA AAGGAGAAAC TGAATTCTGG AATCGAAGTC 
CACCTTGACT GTGAATCCAT CTTTGTGATA GCATCTGTGT TGAAGGATTT TCTGAGAAAT 
ATCCCAGAAA GTATCTTTTC ATCAGATCTA TATGATCACT GGGTCTGTGT AATGGATCAA 
GGAAATGATG AAGAAAAAAT AAATATAATT CAAAGGCTAT TAGATCAGCT TCCCAGAGCC 
AATGTTGTTT TCCTAAGGTA TCTGTTTGGG GTATTACACA ACATTGAACA ACATTCCTTG 
TCTAATCAGA TGACTGCATT TAACTTAGCA GTGTGTATCG CTCCAAGCAT TCTTTGGCCT 
CCTGCTTCCT CCAGTCCAGA GCTAGAAAAT GAATTTACAA AAAAGGTTTC TCTTCTTATA 
CAATTTCTGA TTGAAAACTG CTGTAGGGTA TTTGGAGAAG AAATCGCTTC TCTCTTGGGG 
GAGCTTTCGG AGAGAAGCGA CAGAGAACAC ACCCCAGATA TAACTTGCTT CCAAATGAAC 
GACTCCTCCT ATGACAGCTT GGAAAACGAG CTCAATGAGG AAGCTGATGC TCCATGCAGT 
GACCTGGTCA AAAAGCTTGG CCAGGGTAGC AGGAGCATGG ACTCTGTCTT AACCCTCAGC 
GACTATGACC TTGAACAGCC TGAGGTGGAA GGCCTCTTAA CCCTGAGCAA CTTTGACTTA 
GATCAGTCTA AAGAGGAGCA CATTCCGATA AAGCCACCTC TGGAGCCCAA GCCAGTGAAT 
GTTTTCGTAG GCTACAGGAA GGTTTCACTA GGGGAGCACG CTAGGGCCCC AGCTGGCCCA 
GGCACACTCA GCTGCCTGCC TGTGGCTGCA GCAGATGCCC CGAAAGTCCT GCGGCGGCAC 
CGGCGCTCCT CAGAACCCAG CATTGACTAT CTAGACACAA AGCTTTCCTA TCTCCGGGAG 
TTTTACCAGA AAAAGCTGCG CAAGTCCAGC TGCGATGCTG TCCTCTCTAG AAAAGATGAA 
GATTACCTGA AGCAGACCCA GCCTCAGAAG AAAGGGGATA AGGTGTGTCT TAAACAGAGT 
TCAGTGACAG GCACTGACGT CAGCAAGAGA AACACAGCTA ATGAAAACAT TAAGAAGAAA 
AGCTTGTCTG GTCATGAAGG AACTCAAGTG ACACTTTTTA CTAAGTCCAA GCCAGTACCC 
ATCTCTGTGG CATCTTACAG CCACGGGTCC TCCCAGGATC ATCCCAGGAA GCAAGCCTTT 
GATGCAGACC CATGCCGGTT CTCCCCACCA CACCTAACAG ATGCCCAAAA GAGCTCAAGG 
GTGCAACATC GGCGCTGTTC AGAACCCAGC ATAGATGACC AGAACTACAA GCTGTCCTAT 
CTCAGGGGGA TCTATTCAAT GAAACAAAAT AAAGCCAGCT GTGAGGCTGG CCTCTTGCAT 
GGGGAGGACG ACTATCTCAG AAGGCATAAG TCTTTGCAAA TCGAGGGCCA AAAGCTTATT 
AATCAGAGTT TGGTCATGGG CATTGAGGTG GGCAAGAGCA GCAGCTCTCA CCAAAGCACT 
GAGAAGGTTT TGCCCCCAAG ACTAAACCTT TGCCCAAGGG CAAGCTATTC AAGCTTATCT 
TCTCCAGGCT CGTCCCCATC CGGCTCCTCT GTAAGCTCCC AGGACAGCGC TTTCTCTCAG 
ATCTCTGAAC ACTCCGTGTT TACGCCCACC GAAACCTCCT CACCAATAGA TTGCACTTTC 
CAGACACAAA GAAAACAAGA AGAGCTGTCT TCAGACTTTG ACAGTCCCAG CCGCCTTTCC 
GGAATGCCCG GTCCCTCCAT GGGTCAGGCC AGCAGCCACC TAGCTTATTT AAGAAAGGGT 
ACCACGGAGC AGCCATCACA AATGCATTCT GTAACCCTTC ACCCCAGTGC GTGGTTGAGA 
AGTGGCTTGG TCACTTTAAA AAACTGGTCC CTCAAAAAGA AAACAAAAGC AGCCAGACCA 
GAAGACAGGA AAGTCTGTTC CCTGAAAGAG CCTCTGGAGT TGCCGTCATG TGCTTCCGGT 
ACCCCAGAGG CTGACTCTCT ACAAGAGAGT CAGGATGACT TACAGGGAGA TGAAGGACCT 
GGGCAAACAG CCTGTGGATT CAGTTCTTAT GCCTGTCAGG ACTCAGAGCA ACATGCCGGC 
TCTCCATTCC ACCTGGCAGA AAGCAGACTC AAGCCTTGTA TGAAGTTATA TAAGGGAGAA 
GAGAGTGGAG GGCAGTACCC TTGTGATAAC CCTTGGGAAG GAGCCTCTTC AAGTCTGGAG 
ACCACGGAGG ATACAGCCAA CCCAGGTGCA GAACCTACAA CATTTGCGAT GACGGGGACA 
GATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780744.2
CDS	157..3705
Translation	MEAMSPQQDALGAQPGRSSSLTGMSRIAGGPGTKKKMKTLAERRRSAPSLILDKALQKRPSTRDSHSASIDTCAFLSSFMCSSRTLLIDGPVELKRGLQRQERHLFLFNDLFVSAKIKYNNNFKIKNKIRLTDMWTASCVEEVGEGNMNAQKSFVLGWPTVNFVATFSSPEQKDKWLSLLQRYIALEKEKDYPKSIPLKIFAKDIGNCAYFKTITVMNSDTASEVINMSLQMLGITGSERDYQLWVNSGKEAAPYPLIGHEYPYGIKMSHLRDTALLTQGSRDSASPSQLQEPFLMEQLPREMQCQFILKPTRLATAQQLSDSSQKTFKRRRSIINWAFWRGSSTHLDNLPMSPTSPMPGQLFGVSLPDLCENDNLPKPILDMLSFLNQKGPLTKGIFRQSANMKSCRELKEKLNSGIEVHLDCESIFVIASVLKDFLRNIPESIFSSDLYDHWVCVMDQGNDEEKINIIQRLLDQLPRANVVFLRYLFGVLHNIEQHSLSNQMTAFNLAVCIAPSILWPPASSSPELENEFTKKVSLLIQFLIENCCRVFGEEIASLLGELSERSDREHTPDITCFQMNDSSYDSLENELNEEADAPCSDLVKKLGQGSRSMDSVLTLSDYDLEQPEVEGLLTLSNFDLDQSKEEHIPIKPPLEPKPVNVFVGYRKVSLGEHARAPAGPGTLSCLPVAAADAPKVLRRHRRSSEPSIDYLDTKLSYLREFYQKKLRKSSCDAVLSRKDEDYLKQTQPQKKGDKVCLKQSSVTGTDVSKRNTANENIKKKSLSGHEGTQVTLFTKSKPVPISVASYSHGSSQDHPRKQAFDADPCRFSPPHLTDAQKSSRVQHRRCSEPSIDDQNYKLSYLRGIYSMKQNKASCEAGLLHGEDDYLRRHKSLQIEGQKLINQSLVMGIEVGKSSSSHQSTEKVLPPRLNLCPRASYSSLSSPGSSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQTQRKQEELSSDFDSPSRLSGMPGPSMGQASSHLAYLRKGTTEQPSQMHSVTLHPSAWLRSGLVTLKNWSLKKKTKAARPEDRKVCSLKEPLELPSCASGTPEADSLQESQDDLQGDEGPGQTACGFSSYACQDSEQHAGSPFHLAESRLKPCMKLYKGEESGGQYPCDNPWEGASSSLETTEDTANPGAEPTTFAMTGTDI

Target ORF information:

RefSeq Version	NM_175535.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 20 (Arhgap20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175535.4

ORF Insert Sequence:

ATGGAAGCGA TGTCTCCGCA GCAGGATGCT CTAGGGGCGC AGCCGGGGCG CTCCTCCTCG 
CTGACAGGCA TGTCTCGCAT CGCGGGAGGC CCTGGCACCA AGAAGAAAAT GAAAACACTC 
GCAGAAAGGA GGAGAAGCGC CCCGTCCCTC ATCCTGGACA AAGCCTTACA GAAGCGGCCG 
AGCACCAGAG ACAGCCATTC TGCCAGCATT GACACGTGTG CATTCCTCTC GTCCTTCATG 
TGCTCCAGTC GCACTCTGCT GATTGATGGT CCAGTGGAAC TTAAAAGAGG CCTGCAGAGG 
CAGGAGAGGC ATCTTTTCCT ATTCAACGAT CTGTTTGTCT CAGCCAAAAT CAAATATAAC 
AACAACTTTA AGATAAAGAA TAAAATAAGA CTAACTGACA TGTGGACAGC TAGCTGTGTG 
GAGGAAGTGG GAGAAGGCAA CATGAATGCT CAGAAGTCCT TTGTCCTGGG CTGGCCCACC 
GTGAACTTTG TGGCTACTTT CAGTTCTCCA GAACAAAAGG ACAAATGGCT GTCTCTCCTT 
CAGAGATACA TCGCTCTAGA AAAGGAGAAA GACTACCCGA AGAGCATCCC CCTCAAAATC 
TTTGCCAAGG ACATTGGAAA CTGTGCCTAT TTTAAAACTA TAACAGTGAT GAATTCAGAC 
ACAGCGAGTG AAGTTATCAA CATGTCACTG CAAATGTTAG GAATAACTGG CTCTGAGAGA 
GATTACCAAC TGTGGGTAAA TTCTGGAAAA GAAGCGGCAC CGTACCCACT CATTGGACAC 
GAGTATCCTT ATGGAATTAA AATGAGCCAC CTCCGAGACA CTGCCCTTCT GACGCAGGGA 
TCAAGAGACT CTGCCAGCCC ATCCCAGCTC CAAGAGCCTT TCCTCATGGA GCAGCTGCCC 
CGCGAGATGC AATGCCAGTT CATCCTGAAA CCTACCCGCC TGGCGACAGC CCAGCAACTG 
AGCGATTCCA GCCAGAAGAC ATTTAAAAGG AGGCGCTCTA TCATCAACTG GGCTTTTTGG 
CGGGGTTCTA GCACACACCT GGACAACCTG CCCATGTCAC CAACATCCCC TATGCCAGGA 
CAGCTCTTTG GAGTTTCTTT ACCAGATCTT TGTGAGAACG ACAATCTGCC AAAGCCTATC 
TTGGATATGC TTTCCTTCCT TAACCAAAAA GGCCCCCTCA CAAAAGGCAT CTTCAGGCAG 
TCGGCCAACA TGAAATCTTG CAGAGAGCTA AAGGAGAAAC TGAATTCTGG AATCGAAGTC 
CACCTTGACT GTGAATCCAT CTTTGTGATA GCATCTGTGT TGAAGGATTT TCTGAGAAAT 
ATCCCAGAAA GTATCTTTTC ATCAGATCTA TATGATCACT GGGTCTGTGT AATGGATCAA 
GGAAATGATG AAGAAAAAAT AAATATAATT CAAAGGCTAT TAGATCAGCT TCCCAGAGCC 
AATGTTGTTT TCCTAAGGTA TCTGTTTGGG GTATTACACA ACATTGAACA ACATTCCTTG 
TCTAATCAGA TGACTGCATT TAACTTAGCA GTGTGTATCG CTCCAAGCAT TCTTTGGCCT 
CCTGCTTCCT CCAGTCCAGA GCTAGAAAAT GAATTTACAA AAAAGGTTTC TCTTCTTATA 
CAATTTCTGA TTGAAAACTG CTGTAGGGTA TTTGGAGAAG AAATCGCTTC TCTCTTGGGG 
GAGCTTTCGG AGAGAAGCGA CAGAGAACAC ACCCCAGATA TAACTTGCTT CCAAATGAAC 
GACTCCTCCT ATGACAGCTT GGAAAACGAG CTCAATGAGG AAGCTGATGC TCCATGCAGT 
GACCTGGTCA AAAAGCTTGG CCAGGGTAGC AGGAGCATGG ACTCTGTCTT AACCCTCAGC 
GACTATGACC TTGAACAGCC TGAGGTGGAA GGCCTCTTAA CCCTGAGCAA CTTTGACTTA 
GATCAGTCTA AAGAGGAGCA CATTCCGATA AAGCCACCTC TGGAGCCCAA GCCAGTGAAT 
GTTTTCGTAG GCTACAGGAA GGTTTCACTA GGGGAGCACG CTAGGGCCCC AGCTGGCCCA 
GGCACACTCA GCTGCCTGCC TGTGGCTGCA GCAGATGCCC CGAAAGTCCT GCGGCGGCAC 
CGGCGCTCCT CAGAACCCAG CATTGACTAT CTAGACACAA AGCTTTCCTA TCTCCGGGAG 
TTTTACCAGA AAAAGCTGCG CAAGTCCAGC TGCGATGCTG TCCTCTCTAG AAAAGATGAA 
GATTACCTGA AGCAGACCCA GCCTCAGAAG AAAGGGGATA AGGTGTGTCT TAAACAGAGT 
TCAGTGACAG GCACTGACGT CAGCAAGAGA AACACAGCTA ATGAAAACAT TAAGAAGAAA 
AGCTTGTCTG GTCATGAAGG AACTCAAGTG ACACTTTTTA CTAAGTCCAA GCCAGTACCC 
ATCTCTGTGG CATCTTACAG CCACGGGTCC TCCCAGGATC ATCCCAGGAA GCAAGCCTTT 
GATGCAGACC CATGCCGGTT CTCCCCACCA CACCTAACAG ATGCCCAAAA GAGCTCAAGG 
GTGCAACATC GGCGCTGTTC AGAACCCAGC ATAGATGACC AGAACTACAA GCTGTCCTAT 
CTCAGGGGGA TCTATTCAAT GAAACAAAAT AAAGCCAGCT GTGAGGCTGG CCTCTTGCAT 
GGGGAGGACG ACTATCTCAG AAGGCATAAG TCTTTGCAAA TCGAGGGCCA AAAGCTTATT 
AATCAGAGTT TGGTCATGGG CATTGAGGTG GGCAAGAGCA GCAGCTCTCA CCAAAGCACT 
GAGAAGGTTT TGCCCCCAAG ACTAAACCTT TGCCCAAGGG CAAGCTATTC AAGCTTATCT 
TCTCCAGGCT CGTCCCCATC CGGCTCCTCT GTAAGCTCCC AGGACAGCGC TTTCTCTCAG 
ATCTCTGAAC ACTCCGTGTT TACGCCCACC GAAACCTCCT CACCAATAGA TTGCACTTTC 
CAGACACAAA GAAAACAAGA AGAGCTGTCT TCAGACTTTG ACAGTCCCAG CCGCCTTTCC 
GGAATGCCCG GTCCCTCCAT GGGTCAGGCC AGCAGCCACC TAGCTTATTT AAGAAAGGGT 
ACCACGGAGC AGCCATCACA AATGCATTCT GTAACCCTTC ACCCCAGTGC GTGGTTGAGA 
AGTGGCTTGG TCACTTTAAA AAACTGGTCC CTCAAAAAGA AAACAAAAGC AGCCAGACCA 
GAAGACAGGA AAGTCTGTTC CCTGAAAGAG CCTCTGGAGT TGCCGTCATG TGCTTCCGGT 
ACCCCAGAGG CTGACTCTCT ACAAGAGAGT CAGGATGACT TACAGGGAGA TGAAGGACCT 
GGGCAAACAG CCTGTGGATT CAGTTCTTAT GCCTGTCAGG ACTCAGAGCA ACATGCCGGC 
TCTCCATTCC ACCTGGCAGA AAGCAGACTC AAGCCTTGTA TGAAGTTATA TAAGGGAGAA 
GAGAGTGGAG GGCAGTACCC TTGTGATAAC CCTTGGGAAG GAGCCTCTTC AAGTCTGGAG 
ACCACGGAGG ATACAGCCAA CCCAGGTGCA GAACCTACAA CATTTGCGAT GACGGGGACA 
GATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

cAMP-Epac2-mediated activation of Rap1 in developing male germ cells: RA-RhoGAP as a possible direct down-stream effector.
Molecular reproduction and development76(4)407-16(2009 Apr)
Aivatiadou E,Ripolone M,Brunetti F,Berruti G

A novel group of multi-GAP-domain proteins.
Molecular reproduction and development75(10)1578-89(2008 Oct)
Ruan Y,Sensen CW,van der Hoorn FA

Ovarian gene expression in the absence of FIGLA, an oocyte-specific transcription factor.
BMC developmental biology767(2007 Jun)
Joshi S,Davies H,Sims LP,Levy SE,Dean J

Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2.
Cell129(2)303-17(2007 Apr)
Zhao Y,Ransom JF,Li A,Vedantham V,von Drehle M,Muth AN,Tsuchihashi T,McManus MT,Schwartz RJ,Srivastava D

Characterization of the piRNA complex from rat testes.
Science (New York, N.Y.)313(5785)363-7(2006 Jul)
Lau NC,Seto AG,Kim J,Kuramochi-Miyagawa S,Nakano T,Bartel DP,Kingston RE

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Arhgap20 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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