ARHGAP20 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGAP20
Entrez Gene ID	515501
Full Name	Rho GTPase activating protein 20
General protein information	Preferred Names Rho GTPase activating protein 20 Names rho GTPase-activating protein 20
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001206733.1	NP_001193662.1	rho GTPase-activating protein 20
XM_010812320.2	XP_010810622.1	rho GTPase-activating protein 20 isoform X2
XM_005215783.4	XP_005215840.1	rho GTPase-activating protein 20 isoform X1
XM_024975250.1	XP_024831018.1	rho GTPase-activating protein 20 isoform X2
XM_005215783.3	XP_005215840.1	rho GTPase-activating protein 20 isoform X1
XM_024975249.1	XP_024831017.1	rho GTPase-activating protein 20 isoform X1
NM_001206733.2	NP_001193662.2	rho GTPase-activating protein 20

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARHGAP20	XP_003313436.1
Mus musculus (house mouse)	Arhgap20	NP_780744.2
Gallus gallus (chicken)	ARHGAP20	XP_417154.2
Homo sapiens (human)	ARHGAP20	NP_065860.2
Bos taurus (cattle)	ARHGAP20	NP_001193662.1
	ARHGAP20	XP_001098860.2
Canis lupus familiaris (dog)	ARHGAP20	XP_005619862.1
Rattus norvegicus (Norway rat)	Arhgap20	NP_998794.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ARHGAP20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGAP20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa49304	XM_024975250.1 Latest version!	Bos taurus Rho GTPase activating protein 20 (ARHGAP20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa49304	XM_010812320.2 Latest version!	Bos taurus Rho GTPase activating protein 20 (ARHGAP20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa238910	NM_001206733.2 Latest version!	Bos taurus Rho GTPase activating protein 20 (ARHGAP20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa36039	NM_001206733.1 Latest version!	Bos taurus Rho GTPase activating protein 20 (ARHGAP20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa26195	XM_024975249.1 Latest version!	Bos taurus Rho GTPase activating protein 20 (ARHGAP20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa26195	XM_005215783.4 Latest version!	Bos taurus Rho GTPase activating protein 20 (ARHGAP20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OBa26195	XM_005215783.3	Bos taurus Rho GTPase activating protein 20 (ARHGAP20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGAP20 ( XM_010812320.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810622.1 Bos taurus Rho GTPase activating protein 20 (ARHGAP20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa49304
Clone ID Related Accession (Same CDS sequence)	XM_010812320.2 , XM_024975250.1
Accession Version	XM_010812320.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104407.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812320.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTCT CTCCTTCAAA GTCTAAAACT ATAACAGTAA CGAATTCAGA TACAGCAAAT 
GAAGTTATCA ACATGTCACT ACCAATGCTA GGGATAACTG GCTCTGAGAA AGAGTACCAG 
TTATGGGTCA ATTCTGGCAA AGAAGAGGCA CCATACCCAC TTATTGGGCA TGAATATCCC 
TATGGAATTA AAATGAGCCA TCTTCGAGAC ACTGCACTCC TGACACAGGG ATCAAAGGAC 
TCCCCCACAC CTTCCAACTT CCAAGAGCCC TTCCTCATGG AGCAGCTGCC CCGAGAGATG 
CAGTGTCAGT TCATCCTGAA GCCCAGCCGC CTGGCTGTGA CCCAGCAGCT GAGTGATTCA 
GGTCAGAAGA CATTTAAAAG GAGAAGATCT ATCATAAACT GGGCCTTTTG GCGGGGTTCT 
AGCATTCACC TGGATAACTT GCCTGTGTCA CCAACGTCTC CTATCCCAGG ACAGCTCTTC 
GGGGTTTCTC TGCCAGATAT TTGTGAGAAT GACAATCTGC CCAAAGCTGT CTTGGATATG 
CTTTTATTTC TTAATCAGAA AGGACCTCTC ACAAAAGGCA TCTTCAGACA GTCGGCCAAT 
GTGAAATCCT GCAGAGAGCT GAAAGAAAAA CTGAATTCCG GAGCTGAAGT ACAACTAGAC 
TGTGAATCTA TTTTCGTAAT AGCATCTGTC TTAAAGGATT TTCTACGAAA TATTCCAGGA 
AGTATTTTTT CATCAGATCT CTATGATCAC TGGGTCTGTG TAATGGATCA AGGAAATGAT 
GAAGAGAAAA TAAATGCAGT TCAAAGGCTG TTAGACCAGC TTCCGAGAGC CAATGTTCTT 
CTCCTAAGGT ATCTTTTTGG GTTATTACAC AACATTGAGC AACATTCTTC ATCCAATCAG 
ATGACCGCAT TTAATTTAGC AGTGTGCATC GCCCCAAGCA TTCTTTGGCC TCCTACTTCC 
TCTAGCCCAG AACTAGAAAA CGAATTTACA AAAAAGGTTT CTCTGCTTAT ACAGTTTCTT 
ATTGAAAACT GCTGTCGGAT ATTTGGAGAA GAAATCACTT CCCTCTTGGG AGAGGTTTCA 
GTGAGATGTG ATACCAGAGA GAATGCCTCA GATATCTCTT GCTTCCAGCT GAATGACTCC 
TCCTATGACA GCTTGGAAAA TGAGCTGAAT GACGATGTTG ACGCCCCATG TCGTGACTTG 
GTAAAGAAAC TTGGTCAGGG TAGCAGAAGC ATGGACTCCG TGTTAACCCT CAGTGACTAT 
GACCTTGACC AGCCTGAGGT GGAAGGCCTT TTAACCCTGA GTGACTTTGA CTTAGGCCAT 
TCTAAAGATG AAGACATTCA AATGGGACAG CCCCTTGAGT CCAAGCCAGT GGACACTGCA 
GTGTACACCA AGGTCCCACG CGCCCAGGCC GCCTCTGGCG CGAGCACACC CAGCCGCCTG 
TCCACAGCTG CCGCAGATGC TCCAAAAGGC CTGAGGCGAC ACCGCCGCTG CTCGGAGCCC 
AGCATCGACT ATCTCGATTC AAAGCTTTCC TATCTCAGGG AGTTGTATCA GAAAAAGCTA 
CGCAAGTCCA GCTGTGATGC CATTCTCTCA AAAAAAGATG AGAGCCATCT GAATCAGAAT 
CAACCCCTTC AGGAAGGGGG TAAGACATGT TTTAAAAAGA GTTTAGTCAC AGGCATTGAT 
AGCAAGAAAA ATGCCGCTAG TAAAAATGTT AAGAAGAAAA GTTTGTCTGA TAATGAAGGA 
AGTCATGTAA AACTTTTTCC TAAATCCAAG CCAGTAGCCA TTTCCGTGGC ATCGTACAGT 
CACATGTCCT CCTGTGATCA TCCCAGGAGC CAGCCCTTTG ACATGGATGC ATCCGAATAC 
TCCCCGCCAC ACACTGCAGA CACCCTCAAG AGTTCAAGGA GGCAACGGCG CTGCTCAGAG 
CCCAGCATTG ATGACCAGCA CTGCAGACTG ACCTATCTCA GGGGGATTTA TCCAAAGAAG 
CAGCAGAAAG CCAGCTACGA AGCCAGTCTC TTGCATGGAG AGGAAGATTA TCTCAAGCGG 
CACAAGTCTT TACAAATGGA GGGCCAAAAG CTTATTAATC AGAGTTTGGT CATGGGGATT 
GAGGTGGGCA AGAGTAGTGC CACAAACCAA AACGTGGAGA AGGTTTTACC CCCAAGTTTA 
AACCTTTGCT CAAGGACTAG CTATTCCAGC TTGTCCTCCC CAGGCACTTC CCCATCTGGT 
TCATCAGTAA GCTCCCAAGA TAGTGCTTTT TCTCAGATTT CTGAACACTC TGTGTTTACA 
CCCACTGAAA CATCCTCTCC AATAGATTGT ACTTTTCAAA GAAAACAGGC AGAGCTTTCT 
TCTGACTTTA GCAGTTCTGG CCTCATTTCT GGAATTCCTG GTCCCTCATC AGGGCAGGCC 
AGCGGCCGCC TGGCCTATAC AAAAAAGGAC AGCGTAGAGT GGCATTCACA CATGCATTCT 
GTAACTCTTC ACCCCAGCAC ATGGTTGAAG AGTGGTGTAG CCAGTTTGAA AAACTGGTCT 
CTCAAAAAGA AGGCAAGGGC ACCCAGACCA GAGGAAAATA AAGTGGGTTC TCTAAAAGGA 
CCCACGGATC CTCCTGCACA TGCTTCTGGT GTTCCGGAAG CCATCACACT ACAAGAAAGG 
CAGAAAGACG TGCCCCAAAG GGCAGCTGAA GGACTGGGAG CTGTTCAGAC GGCCCGCTGG 
TATAGTTCCT ACCCCAGCCA GGACTCAGAG AAATACTGTA GCTCTCTGTT CAGCCTCGTG 
GAAGACAGAC TTGGGCTTCA TATGAAGTCG CCCGAGGAAG GAAAGGATAG CGGGCAGTGC 
TCTCCTGGTA TGCTGCCCCA GAGAAGATCC TCCCCCGGCT CTTTGACTGT GGATGATGCG 
TCCAGCCCAG ACTCAGGGCC GCCCGTGGTG TGTGATGCTG AGGATAGACA TTTGGCCAAA 
GACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810622.1
CDS	122..3070
Translation	MALSPSKSKTITVTNSDTANEVINMSLPMLGITGSEKEYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDTALLTQGSKDSPTPSNFQEPFLMEQLPREMQCQFILKPSRLAVTQQLSDSGQKTFKRRRSIINWAFWRGSSIHLDNLPVSPTSPIPGQLFGVSLPDICENDNLPKAVLDMLLFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGAEVQLDCESIFVIASVLKDFLRNIPGSIFSSDLYDHWVCVMDQGNDEEKINAVQRLLDQLPRANVLLLRYLFGLLHNIEQHSSSNQMTAFNLAVCIAPSILWPPTSSSPELENEFTKKVSLLIQFLIENCCRIFGEEITSLLGEVSVRCDTRENASDISCFQLNDSSYDSLENELNDDVDAPCRDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLGHSKDEDIQMGQPLESKPVDTAVYTKVPRAQAASGASTPSRLSTAAADAPKGLRRHRRCSEPSIDYLDSKLSYLRELYQKKLRKSSCDAILSKKDESHLNQNQPLQEGGKTCFKKSLVTGIDSKKNAASKNVKKKSLSDNEGSHVKLFPKSKPVAISVASYSHMSSCDHPRSQPFDMDASEYSPPHTADTLKSSRRQRRCSEPSIDDQHCRLTYLRGIYPKKQQKASYEASLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNVEKVLPPSLNLCSRTSYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQRKQAELSSDFSSSGLISGIPGPSSGQASGRLAYTKKDSVEWHSHMHSVTLHPSTWLKSGVASLKNWSLKKKARAPRPEENKVGSLKGPTDPPAHASGVPEAITLQERQKDVPQRAAEGLGAVQTARWYSSYPSQDSEKYCSSLFSLVEDRLGLHMKSPEEGKDSGQCSPGMLPQRRSSPGSLTVDDASSPDSGPPVVCDAEDRHLAKDM

Target ORF information:

RefSeq Version	XM_010812320.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 20 (ARHGAP20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812320.2

ORF Insert Sequence:

ATGGCTCTCT CTCCTTCAAA GTCTAAAACT ATAACAGTAA CGAATTCAGA TACAGCAAAT 
GAAGTTATCA ACATGTCACT ACCAATGCTA GGGATAACTG GCTCTGAGAA AGAGTACCAG 
TTATGGGTCA ATTCTGGCAA AGAAGAGGCA CCATACCCAC TTATTGGGCA TGAATATCCC 
TATGGAATTA AAATGAGCCA TCTTCGAGAC ACTGCACTCC TGACACAGGG ATCAAAGGAC 
TCCCCCACAC CTTCCAACTT CCAAGAGCCC TTCCTCATGG AGCAGCTGCC CCGAGAGATG 
CAGTGTCAGT TCATCCTGAA GCCCAGCCGC CTGGCTGTGA CCCAGCAGCT GAGTGATTCA 
GGTCAGAAGA CATTTAAAAG GAGAAGATCT ATCATAAACT GGGCCTTTTG GCGGGGTTCT 
AGCATTCACC TGGATAACTT GCCTGTGTCA CCAACGTCTC CTATCCCAGG ACAGCTCTTC 
GGGGTTTCTC TGCCAGATAT TTGTGAGAAT GACAATCTGC CCAAAGCTGT CTTGGATATG 
CTTTTATTTC TTAATCAGAA AGGACCTCTC ACAAAAGGCA TCTTCAGACA GTCGGCCAAT 
GTGAAATCCT GCAGAGAGCT GAAAGAAAAA CTGAATTCCG GAGCTGAAGT ACAACTAGAC 
TGTGAATCTA TTTTCGTAAT AGCATCTGTC TTAAAGGATT TTCTACGAAA TATTCCAGGA 
AGTATTTTTT CATCAGATCT CTATGATCAC TGGGTCTGTG TAATGGATCA AGGAAATGAT 
GAAGAGAAAA TAAATGCAGT TCAAAGGCTG TTAGACCAGC TTCCGAGAGC CAATGTTCTT 
CTCCTAAGGT ATCTTTTTGG GTTATTACAC AACATTGAGC AACATTCTTC ATCCAATCAG 
ATGACCGCAT TTAATTTAGC AGTGTGCATC GCCCCAAGCA TTCTTTGGCC TCCTACTTCC 
TCTAGCCCAG AACTAGAAAA CGAATTTACA AAAAAGGTTT CTCTGCTTAT ACAGTTTCTT 
ATTGAAAACT GCTGTCGGAT ATTTGGAGAA GAAATCACTT CCCTCTTGGG AGAGGTTTCA 
GTGAGATGTG ATACCAGAGA GAATGCCTCA GATATCTCTT GCTTCCAGCT GAATGACTCC 
TCCTATGACA GCTTGGAAAA TGAGCTGAAT GACGATGTTG ACGCCCCATG TCGTGACTTG 
GTAAAGAAAC TTGGTCAGGG TAGCAGAAGC ATGGACTCCG TGTTAACCCT CAGTGACTAT 
GACCTTGACC AGCCTGAGGT GGAAGGCCTT TTAACCCTGA GTGACTTTGA CTTAGGCCAT 
TCTAAAGATG AAGACATTCA AATGGGACAG CCCCTTGAGT CCAAGCCAGT GGACACTGCA 
GTGTACACCA AGGTCCCACG CGCCCAGGCC GCCTCTGGCG CGAGCACACC CAGCCGCCTG 
TCCACAGCTG CCGCAGATGC TCCAAAAGGC CTGAGGCGAC ACCGCCGCTG CTCGGAGCCC 
AGCATCGACT ATCTCGATTC AAAGCTTTCC TATCTCAGGG AGTTGTATCA GAAAAAGCTA 
CGCAAGTCCA GCTGTGATGC CATTCTCTCA AAAAAAGATG AGAGCCATCT GAATCAGAAT 
CAACCCCTTC AGGAAGGGGG TAAGACATGT TTTAAAAAGA GTTTAGTCAC AGGCATTGAT 
AGCAAGAAAA ATGCCGCTAG TAAAAATGTT AAGAAGAAAA GTTTGTCTGA TAATGAAGGA 
AGTCATGTAA AACTTTTTCC TAAATCCAAG CCAGTAGCCA TTTCCGTGGC ATCGTACAGT 
CACATGTCCT CCTGTGATCA TCCCAGGAGC CAGCCCTTTG ACATGGATGC ATCCGAATAC 
TCCCCGCCAC ACACTGCAGA CACCCTCAAG AGTTCAAGGA GGCAACGGCG CTGCTCAGAG 
CCCAGCATTG ATGACCAGCA CTGCAGACTG ACCTATCTCA GGGGGATTTA TCCAAAGAAG 
CAGCAGAAAG CCAGCTACGA AGCCAGTCTC TTGCATGGAG AGGAAGATTA TCTCAAGCGG 
CACAAGTCTT TACAAATGGA GGGCCAAAAG CTTATTAATC AGAGTTTGGT CATGGGGATT 
GAGGTGGGCA AGAGTAGTGC CACAAACCAA AACGTGGAGA AGGTTTTACC CCCAAGTTTA 
AACCTTTGCT CAAGGACTAG CTATTCCAGC TTGTCCTCCC CAGGCACTTC CCCATCTGGT 
TCATCAGTAA GCTCCCAAGA TAGTGCTTTT TCTCAGATTT CTGAACACTC TGTGTTTACA 
CCCACTGAAA CATCCTCTCC AATAGATTGT ACTTTTCAAA GAAAACAGGC AGAGCTTTCT 
TCTGACTTTA GCAGTTCTGG CCTCATTTCT GGAATTCCTG GTCCCTCATC AGGGCAGGCC 
AGCGGCCGCC TGGCCTATAC AAAAAAGGAC AGCGTAGAGT GGCATTCACA CATGCATTCT 
GTAACTCTTC ACCCCAGCAC ATGGTTGAAG AGTGGTGTAG CCAGTTTGAA AAACTGGTCT 
CTCAAAAAGA AGGCAAGGGC ACCCAGACCA GAGGAAAATA AAGTGGGTTC TCTAAAAGGA 
CCCACGGATC CTCCTGCACA TGCTTCTGGT GTTCCGGAAG CCATCACACT ACAAGAAAGG 
CAGAAAGACG TGCCCCAAAG GGCAGCTGAA GGACTGGGAG CTGTTCAGAC GGCCCGCTGG 
TATAGTTCCT ACCCCAGCCA GGACTCAGAG AAATACTGTA GCTCTCTGTT CAGCCTCGTG 
GAAGACAGAC TTGGGCTTCA TATGAAGTCG CCCGAGGAAG GAAAGGATAG CGGGCAGTGC 
TCTCCTGGTA TGCTGCCCCA GAGAAGATCC TCCCCCGGCT CTTTGACTGT GGATGATGCG 
TCCAGCCCAG ACTCAGGGCC GCCCGTGGTG TGTGATGCTG AGGATAGACA TTTGGCCAAA 
GACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP20 ( XM_024975250.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831018.1 Bos taurus Rho GTPase activating protein 20 (ARHGAP20), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa49304
Clone ID Related Accession (Same CDS sequence)	XM_010812320.2 , XM_024975250.1
Accession Version	XM_024975250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTCT CTCCTTCAAA GTCTAAAACT ATAACAGTAA CGAATTCAGA TACAGCAAAT 
GAAGTTATCA ACATGTCACT ACCAATGCTA GGGATAACTG GCTCTGAGAA AGAGTACCAG 
TTATGGGTCA ATTCTGGCAA AGAAGAGGCA CCATACCCAC TTATTGGGCA TGAATATCCC 
TATGGAATTA AAATGAGCCA TCTTCGAGAC ACTGCACTCC TGACACAGGG ATCAAAGGAC 
TCCCCCACAC CTTCCAACTT CCAAGAGCCC TTCCTCATGG AGCAGCTGCC CCGAGAGATG 
CAGTGTCAGT TCATCCTGAA GCCCAGCCGC CTGGCTGTGA CCCAGCAGCT GAGTGATTCA 
GGTCAGAAGA CATTTAAAAG GAGAAGATCT ATCATAAACT GGGCCTTTTG GCGGGGTTCT 
AGCATTCACC TGGATAACTT GCCTGTGTCA CCAACGTCTC CTATCCCAGG ACAGCTCTTC 
GGGGTTTCTC TGCCAGATAT TTGTGAGAAT GACAATCTGC CCAAAGCTGT CTTGGATATG 
CTTTTATTTC TTAATCAGAA AGGACCTCTC ACAAAAGGCA TCTTCAGACA GTCGGCCAAT 
GTGAAATCCT GCAGAGAGCT GAAAGAAAAA CTGAATTCCG GAGCTGAAGT ACAACTAGAC 
TGTGAATCTA TTTTCGTAAT AGCATCTGTC TTAAAGGATT TTCTACGAAA TATTCCAGGA 
AGTATTTTTT CATCAGATCT CTATGATCAC TGGGTCTGTG TAATGGATCA AGGAAATGAT 
GAAGAGAAAA TAAATGCAGT TCAAAGGCTG TTAGACCAGC TTCCGAGAGC CAATGTTCTT 
CTCCTAAGGT ATCTTTTTGG GTTATTACAC AACATTGAGC AACATTCTTC ATCCAATCAG 
ATGACCGCAT TTAATTTAGC AGTGTGCATC GCCCCAAGCA TTCTTTGGCC TCCTACTTCC 
TCTAGCCCAG AACTAGAAAA CGAATTTACA AAAAAGGTTT CTCTGCTTAT ACAGTTTCTT 
ATTGAAAACT GCTGTCGGAT ATTTGGAGAA GAAATCACTT CCCTCTTGGG AGAGGTTTCA 
GTGAGATGTG ATACCAGAGA GAATGCCTCA GATATCTCTT GCTTCCAGCT GAATGACTCC 
TCCTATGACA GCTTGGAAAA TGAGCTGAAT GACGATGTTG ACGCCCCATG TCGTGACTTG 
GTAAAGAAAC TTGGTCAGGG TAGCAGAAGC ATGGACTCCG TGTTAACCCT CAGTGACTAT 
GACCTTGACC AGCCTGAGGT GGAAGGCCTT TTAACCCTGA GTGACTTTGA CTTAGGCCAT 
TCTAAAGATG AAGACATTCA AATGGGACAG CCCCTTGAGT CCAAGCCAGT GGACACTGCA 
GTGTACACCA AGGTCCCACG CGCCCAGGCC GCCTCTGGCG CGAGCACACC CAGCCGCCTG 
TCCACAGCTG CCGCAGATGC TCCAAAAGGC CTGAGGCGAC ACCGCCGCTG CTCGGAGCCC 
AGCATCGACT ATCTCGATTC AAAGCTTTCC TATCTCAGGG AGTTGTATCA GAAAAAGCTA 
CGCAAGTCCA GCTGTGATGC CATTCTCTCA AAAAAAGATG AGAGCCATCT GAATCAGAAT 
CAACCCCTTC AGGAAGGGGG TAAGACATGT TTTAAAAAGA GTTTAGTCAC AGGCATTGAT 
AGCAAGAAAA ATGCCGCTAG TAAAAATGTT AAGAAGAAAA GTTTGTCTGA TAATGAAGGA 
AGTCATGTAA AACTTTTTCC TAAATCCAAG CCAGTAGCCA TTTCCGTGGC ATCGTACAGT 
CACATGTCCT CCTGTGATCA TCCCAGGAGC CAGCCCTTTG ACATGGATGC ATCCGAATAC 
TCCCCGCCAC ACACTGCAGA CACCCTCAAG AGTTCAAGGA GGCAACGGCG CTGCTCAGAG 
CCCAGCATTG ATGACCAGCA CTGCAGACTG ACCTATCTCA GGGGGATTTA TCCAAAGAAG 
CAGCAGAAAG CCAGCTACGA AGCCAGTCTC TTGCATGGAG AGGAAGATTA TCTCAAGCGG 
CACAAGTCTT TACAAATGGA GGGCCAAAAG CTTATTAATC AGAGTTTGGT CATGGGGATT 
GAGGTGGGCA AGAGTAGTGC CACAAACCAA AACGTGGAGA AGGTTTTACC CCCAAGTTTA 
AACCTTTGCT CAAGGACTAG CTATTCCAGC TTGTCCTCCC CAGGCACTTC CCCATCTGGT 
TCATCAGTAA GCTCCCAAGA TAGTGCTTTT TCTCAGATTT CTGAACACTC TGTGTTTACA 
CCCACTGAAA CATCCTCTCC AATAGATTGT ACTTTTCAAA GAAAACAGGC AGAGCTTTCT 
TCTGACTTTA GCAGTTCTGG CCTCATTTCT GGAATTCCTG GTCCCTCATC AGGGCAGGCC 
AGCGGCCGCC TGGCCTATAC AAAAAAGGAC AGCGTAGAGT GGCATTCACA CATGCATTCT 
GTAACTCTTC ACCCCAGCAC ATGGTTGAAG AGTGGTGTAG CCAGTTTGAA AAACTGGTCT 
CTCAAAAAGA AGGCAAGGGC ACCCAGACCA GAGGAAAATA AAGTGGGTTC TCTAAAAGGA 
CCCACGGATC CTCCTGCACA TGCTTCTGGT GTTCCGGAAG CCATCACACT ACAAGAAAGG 
CAGAAAGACG TGCCCCAAAG GGCAGCTGAA GGACTGGGAG CTGTTCAGAC GGCCCGCTGG 
TATAGTTCCT ACCCCAGCCA GGACTCAGAG AAATACTGTA GCTCTCTGTT CAGCCTCGTG 
GAAGACAGAC TTGGGCTTCA TATGAAGTCG CCCGAGGAAG GAAAGGATAG CGGGCAGTGC 
TCTCCTGGTA TGCTGCCCCA GAGAAGATCC TCCCCCGGCT CTTTGACTGT GGATGATGCG 
TCCAGCCCAG ACTCAGGGCC GCCCGTGGTG TGTGATGCTG AGGATAGACA TTTGGCCAAA 
GACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831018.1
CDS	701..3649
Translation	MALSPSKSKTITVTNSDTANEVINMSLPMLGITGSEKEYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDTALLTQGSKDSPTPSNFQEPFLMEQLPREMQCQFILKPSRLAVTQQLSDSGQKTFKRRRSIINWAFWRGSSIHLDNLPVSPTSPIPGQLFGVSLPDICENDNLPKAVLDMLLFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGAEVQLDCESIFVIASVLKDFLRNIPGSIFSSDLYDHWVCVMDQGNDEEKINAVQRLLDQLPRANVLLLRYLFGLLHNIEQHSSSNQMTAFNLAVCIAPSILWPPTSSSPELENEFTKKVSLLIQFLIENCCRIFGEEITSLLGEVSVRCDTRENASDISCFQLNDSSYDSLENELNDDVDAPCRDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLGHSKDEDIQMGQPLESKPVDTAVYTKVPRAQAASGASTPSRLSTAAADAPKGLRRHRRCSEPSIDYLDSKLSYLRELYQKKLRKSSCDAILSKKDESHLNQNQPLQEGGKTCFKKSLVTGIDSKKNAASKNVKKKSLSDNEGSHVKLFPKSKPVAISVASYSHMSSCDHPRSQPFDMDASEYSPPHTADTLKSSRRQRRCSEPSIDDQHCRLTYLRGIYPKKQQKASYEASLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNVEKVLPPSLNLCSRTSYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQRKQAELSSDFSSSGLISGIPGPSSGQASGRLAYTKKDSVEWHSHMHSVTLHPSTWLKSGVASLKNWSLKKKARAPRPEENKVGSLKGPTDPPAHASGVPEAITLQERQKDVPQRAAEGLGAVQTARWYSSYPSQDSEKYCSSLFSLVEDRLGLHMKSPEEGKDSGQCSPGMLPQRRSSPGSLTVDDASSPDSGPPVVCDAEDRHLAKDM

Target ORF information:

RefSeq Version	XM_024975250.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 20 (ARHGAP20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975250.1

ORF Insert Sequence:

ATGGCTCTCT CTCCTTCAAA GTCTAAAACT ATAACAGTAA CGAATTCAGA TACAGCAAAT 
GAAGTTATCA ACATGTCACT ACCAATGCTA GGGATAACTG GCTCTGAGAA AGAGTACCAG 
TTATGGGTCA ATTCTGGCAA AGAAGAGGCA CCATACCCAC TTATTGGGCA TGAATATCCC 
TATGGAATTA AAATGAGCCA TCTTCGAGAC ACTGCACTCC TGACACAGGG ATCAAAGGAC 
TCCCCCACAC CTTCCAACTT CCAAGAGCCC TTCCTCATGG AGCAGCTGCC CCGAGAGATG 
CAGTGTCAGT TCATCCTGAA GCCCAGCCGC CTGGCTGTGA CCCAGCAGCT GAGTGATTCA 
GGTCAGAAGA CATTTAAAAG GAGAAGATCT ATCATAAACT GGGCCTTTTG GCGGGGTTCT 
AGCATTCACC TGGATAACTT GCCTGTGTCA CCAACGTCTC CTATCCCAGG ACAGCTCTTC 
GGGGTTTCTC TGCCAGATAT TTGTGAGAAT GACAATCTGC CCAAAGCTGT CTTGGATATG 
CTTTTATTTC TTAATCAGAA AGGACCTCTC ACAAAAGGCA TCTTCAGACA GTCGGCCAAT 
GTGAAATCCT GCAGAGAGCT GAAAGAAAAA CTGAATTCCG GAGCTGAAGT ACAACTAGAC 
TGTGAATCTA TTTTCGTAAT AGCATCTGTC TTAAAGGATT TTCTACGAAA TATTCCAGGA 
AGTATTTTTT CATCAGATCT CTATGATCAC TGGGTCTGTG TAATGGATCA AGGAAATGAT 
GAAGAGAAAA TAAATGCAGT TCAAAGGCTG TTAGACCAGC TTCCGAGAGC CAATGTTCTT 
CTCCTAAGGT ATCTTTTTGG GTTATTACAC AACATTGAGC AACATTCTTC ATCCAATCAG 
ATGACCGCAT TTAATTTAGC AGTGTGCATC GCCCCAAGCA TTCTTTGGCC TCCTACTTCC 
TCTAGCCCAG AACTAGAAAA CGAATTTACA AAAAAGGTTT CTCTGCTTAT ACAGTTTCTT 
ATTGAAAACT GCTGTCGGAT ATTTGGAGAA GAAATCACTT CCCTCTTGGG AGAGGTTTCA 
GTGAGATGTG ATACCAGAGA GAATGCCTCA GATATCTCTT GCTTCCAGCT GAATGACTCC 
TCCTATGACA GCTTGGAAAA TGAGCTGAAT GACGATGTTG ACGCCCCATG TCGTGACTTG 
GTAAAGAAAC TTGGTCAGGG TAGCAGAAGC ATGGACTCCG TGTTAACCCT CAGTGACTAT 
GACCTTGACC AGCCTGAGGT GGAAGGCCTT TTAACCCTGA GTGACTTTGA CTTAGGCCAT 
TCTAAAGATG AAGACATTCA AATGGGACAG CCCCTTGAGT CCAAGCCAGT GGACACTGCA 
GTGTACACCA AGGTCCCACG CGCCCAGGCC GCCTCTGGCG CGAGCACACC CAGCCGCCTG 
TCCACAGCTG CCGCAGATGC TCCAAAAGGC CTGAGGCGAC ACCGCCGCTG CTCGGAGCCC 
AGCATCGACT ATCTCGATTC AAAGCTTTCC TATCTCAGGG AGTTGTATCA GAAAAAGCTA 
CGCAAGTCCA GCTGTGATGC CATTCTCTCA AAAAAAGATG AGAGCCATCT GAATCAGAAT 
CAACCCCTTC AGGAAGGGGG TAAGACATGT TTTAAAAAGA GTTTAGTCAC AGGCATTGAT 
AGCAAGAAAA ATGCCGCTAG TAAAAATGTT AAGAAGAAAA GTTTGTCTGA TAATGAAGGA 
AGTCATGTAA AACTTTTTCC TAAATCCAAG CCAGTAGCCA TTTCCGTGGC ATCGTACAGT 
CACATGTCCT CCTGTGATCA TCCCAGGAGC CAGCCCTTTG ACATGGATGC ATCCGAATAC 
TCCCCGCCAC ACACTGCAGA CACCCTCAAG AGTTCAAGGA GGCAACGGCG CTGCTCAGAG 
CCCAGCATTG ATGACCAGCA CTGCAGACTG ACCTATCTCA GGGGGATTTA TCCAAAGAAG 
CAGCAGAAAG CCAGCTACGA AGCCAGTCTC TTGCATGGAG AGGAAGATTA TCTCAAGCGG 
CACAAGTCTT TACAAATGGA GGGCCAAAAG CTTATTAATC AGAGTTTGGT CATGGGGATT 
GAGGTGGGCA AGAGTAGTGC CACAAACCAA AACGTGGAGA AGGTTTTACC CCCAAGTTTA 
AACCTTTGCT CAAGGACTAG CTATTCCAGC TTGTCCTCCC CAGGCACTTC CCCATCTGGT 
TCATCAGTAA GCTCCCAAGA TAGTGCTTTT TCTCAGATTT CTGAACACTC TGTGTTTACA 
CCCACTGAAA CATCCTCTCC AATAGATTGT ACTTTTCAAA GAAAACAGGC AGAGCTTTCT 
TCTGACTTTA GCAGTTCTGG CCTCATTTCT GGAATTCCTG GTCCCTCATC AGGGCAGGCC 
AGCGGCCGCC TGGCCTATAC AAAAAAGGAC AGCGTAGAGT GGCATTCACA CATGCATTCT 
GTAACTCTTC ACCCCAGCAC ATGGTTGAAG AGTGGTGTAG CCAGTTTGAA AAACTGGTCT 
CTCAAAAAGA AGGCAAGGGC ACCCAGACCA GAGGAAAATA AAGTGGGTTC TCTAAAAGGA 
CCCACGGATC CTCCTGCACA TGCTTCTGGT GTTCCGGAAG CCATCACACT ACAAGAAAGG 
CAGAAAGACG TGCCCCAAAG GGCAGCTGAA GGACTGGGAG CTGTTCAGAC GGCCCGCTGG 
TATAGTTCCT ACCCCAGCCA GGACTCAGAG AAATACTGTA GCTCTCTGTT CAGCCTCGTG 
GAAGACAGAC TTGGGCTTCA TATGAAGTCG CCCGAGGAAG GAAAGGATAG CGGGCAGTGC 
TCTCCTGGTA TGCTGCCCCA GAGAAGATCC TCCCCCGGCT CTTTGACTGT GGATGATGCG 
TCCAGCCCAG ACTCAGGGCC GCCCGTGGTG TGTGATGCTG AGGATAGACA TTTGGCCAAA 
GACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP20 ( NM_001206733.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193662.2 Bos taurus Rho GTPase activating protein 20 (ARHGAP20), mRNA.

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CloneID	OBa238910
Clone ID Related Accession (Same CDS sequence)	NM_001206733.2
Accession Version	NM_001206733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 20
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000015.1. On May 29, 2018 this sequence version replaced NM_001206733.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145491 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGCGA TGTCCCCCCA GCAGGAGACT CTAGGGGGAC AGCCGGGGCG CTCCTCTTCC 
CTGACAGGAG TGTCTCGGAT CGCAGGCGGC CCCGGCACGA AGAAGAAAAT GAAAACATTA 
GCAGAAAGGA GGAGGAGTGC TCCATCTCTT ATCCTGGATA AAGCCCTGCA AAAGCGGCCC 
AGTACCAAAG AAAGTCCTTC TGCTAGCCTG GACACATGTG CATTTCTGTC ATCATTTGTG 
TGCTCCAGTA GGACTCTGCT TATTGATGGC CGTGTAGAAC TCAAGAGAGG CCTGCAGAGG 
CAGGAGCGAC ATCTTTTCCT GTTCAATGAT CTGTTTGTTG TGGCCAAAAT CAAATATAAC 
AATAACTTTA AGATAAAAAA TAAAATAAAA TTAAGTGACA TGTGGACAGC AAGCTGTGTG 
GATGAAGTGG GAGAAGGCAA CACCAATGCC ACGAAATCCT TTGTCTTGGG CTGGCCCACA 
GTGAACTTCG TGGCCACTTT CAGTTCTCCA GAACAGAAGG ACAAATGGCT CTCTCTCCTT 
CAAAGATACA TCAATCTAGA GAAAGAAAAG GACTACCCGA AGAGCATTCC CCTCAAAATC 
TTCGCCAAGG ACATTGGGAA TTGTGCCTAT TCTAAAACTA TAACAGTAAC GAATTCAGAT 
ACAGCAAATG AAGTTATCAA CATGTCACTA CCAATGCTAG GGATAACTGG CTCTGAGAAA 
GAGTACCAGT TATGGGTCAA TTCTGGCAAA GAAGAGGCAC CATACCCACT TATTGGGCAT 
GAATATCCCT ATGGAATTAA AATGAGCCAT CTTCGAGACA CTGCACTCCT GACACAGGGA 
TCAAAGGACT CCCCCACACC TTCCAACTTC CAAGAGCCCT TCCTCATGGA GCAGCTGCCC 
CGAGAGATGC AGTGTCAGTT CATCCTGAAG CCCAGCCGCC TGGCTGTGAC CCAGCAGCTG 
AGTGATTCAG GTCAGAAGAC ATTTAAAAGG AGAAGATCTA TCATAAACTG GGCCTTTTGG 
CGGGGTTCTA GCATTCACCT GGATAACTTG CCTGTGTCAC CAACGTCTCC TATCCCAGGA 
CAGCTCTTCG GGGTTTCTCT GCCAGATATT TGTGAGAATG ACAATCTGCC CAAAGCTGTC 
TTGGATATGC TTTTATTTCT TAATCAGAAA GGACCTCTCA CAAAAGGCAT CTTCAGACAG 
TCGGCCAATG TGAAATCCTG CAGAGAGCTG AAAGAAAAAC TGAATTCCGG AGCTGAAGTA 
CAACTAGACT GTGAATCTAT TTTCGTAATA GCATCTGTCT TAAAGGATTT TCTACGAAAT 
ATTCCAGGAA GTATTTTTTC ATCAGATCTC TATGATCACT GGGTCTGTGT AATGGATCAA 
GGAAATGATG AAGAGAAAAT AAATGCAGTT CAAAGGCTGT TAGACCAGCT TCCGAGAGCC 
AATGTTCTTC TCCTAAGGTA TCTTTTTGGG TTATTACACA ACATTGAGCA ACATTCTTCA 
TCCAATCAGA TGACCGCATT TAATTTAGCA GTGTGCATCG CCCCAAGCAT TCTTTGGCCT 
CCTACTTCCT CTAGCCCAGA ACTAGAAAAC GAATTTACAA AAAAGGTTTC TCTGCTTATA 
CAGTTTCTTA TTGAAAACTG CTGTCGGATA TTTGGAGAAG AAATCACTTC CCTCTTGGGA 
GAGGTTTCAG TGAGATGTGA TACCAGAGAG AATGCCTCAG ATATCTCTTG CTTCCAGCTG 
AATGACTCCT CCTATGACAG CTTGGAAAAT GAGCTGAATG ACGATGTTGA CGCCCCATGT 
CGTGACTTGG TAAAGAAACT TGGTCAGGGT AGCAGAAGCA TGGACTCCGT GTTAACCCTC 
AGTGACTATG ACCTTGACCA GCCTGAGGTG GAAGGCCTTT TAACCCTGAG TGACTTTGAC 
TTAGGCCATT CTAAAGATGA AGACATTCAA ATGGGACAGC CCCTTGAGTC CAAGCCAGTG 
GACACTGCAG TGTACACCAA GGTCCCACGC GCCCAGGCCG CCTCTGGCGC GAGCACACCC 
AGCCGCCTGT CCACAGCTGC CGCAGATGCT CCAAAAGGCC TGAGGCGACA CCGCCGCTGC 
TCGGAGCCCA GCATCGACTA TCTCGATTCA AAGCTTTCCT ATCTCAGGGA GTTGTATCAG 
AAAAAGCTAC GCAAGTCCAG CTGTGATGCC ATTCTCTCAA AAAAAGATGA GAGCCATCTG 
AATCAGAATC AACCCCTTCA GGAAGGGGGT AAGACATGTT TTAAAAAGAG TTTAGTCACA 
GGCATTGATA GCAAGAAAAA TGCCGCTAGT AAAAATGTTA AGAAGAAAAG TTTGTCTGAT 
AATGAAGGAA GTCATGTAAA ACTTTTTCCT AAATCCAAGC CAGTAGCCAT TTCCGTGGCA 
TCGTACAGTC ACATGTCCTC CTGTGATCAT CCCAGGAGCC AGCCCTTTGA CATGGATGCA 
TCCGAATACT CCCCGCCACA CACTGCAGAC ACCCTCAAGA GTTCAAGGAG GCAACGGCGC 
TGCTCAGAGC CCAGCATTGA TGACCAGCAC TGCAGACTGA CCTATCTCAG GGGGATTTAT 
CCAAAGAAGC AGCAGAAAGC CAGCTACGAA GCCAGTCTCT TGCATGGAGA GGAAGATTAT 
CTCAAGCGGC ACAAGTCTTT ACAAATGGAG GGCCAAAAGC TTATTAATCA GAGTTTGGTC 
ATGGGGATTG AGGTGGGCAA GAGTAGTGCC ACAAACCAAA ACGTGGAGAA GGTTTTACCC 
CCAAGTTTAA ACCTTTGCTC AAGGACTAGC TATTCCAGCT TGTCCTCCCC AGGCACTTCC 
CCATCTGGTT CATCAGTAAG CTCCCAAGAT AGTGCTTTTT CTCAGATTTC TGAACACTCT 
GTGTTTACAC CCACTGAAAC ATCCTCTCCA ATAGATTGTA CTTTTCAAAG AAAACAGGCA 
GAGCTTTCTT CTGACTTTAG CAGTTCTGGC CTCATTTCTG GAATTCCTGG TCCCTCATCA 
GGGCAGGCCA GCGGCCGCCT GGCCTATACA AAAAAGGACA GCGTAGAGTG GCATTCACAC 
ATGCATTCTG TAACTCTTCA CCCCAGCACA TGGTTGAAGA GTGGTGTAGC CAGTTTGAAA 
AACTGGTCTC TCAAAAAGAA GGCAAGGGCA CCCAGACCAG AGGAAAATAA AGTGGGTTCT 
CTAAAAGGAC CCACGGATCC TCCTGCACAT GCTTCTGGTG TTCCGGAAGC CATCACACTA 
CAAGAAAGGC AGAAAGACGT GCCCCAAAGG GCAGCTGAAG GACTGGGAGC TGTTCAGACG 
GCCCGCTGGT ATAGTTCCTA CCCCAGCCAG GACTCAGAGA AATACTGTAG CTCTCTGTTC 
AGCCTCGTGG AAGACAGACT TGGGCTTCAT ATGAAGTCGC CCGAGGAAGG AAAGGATAGC 
GGGCAGTGCT CTCCTGGTAT GCTGCCCCAG AGAAGATCCT CCCCCGGCTC TTTGACTGTG 
GATGATGCGT CCAGCCCAGA CTCAGGGCCG CCCGTGGTGT GTGATGCTGA GGATAGACAT 
TTGGCCAAAG ACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193662.2
CDS	1..3558
Translation	MEAMSPQQETLGGQPGRSSSLTGVSRIAGGPGTKKKMKTLAERRRSAPSLILDKALQKRPSTKESPSASLDTCAFLSSFVCSSRTLLIDGRVELKRGLQRQERHLFLFNDLFVVAKIKYNNNFKIKNKIKLSDMWTASCVDEVGEGNTNATKSFVLGWPTVNFVATFSSPEQKDKWLSLLQRYINLEKEKDYPKSIPLKIFAKDIGNCAYSKTITVTNSDTANEVINMSLPMLGITGSEKEYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDTALLTQGSKDSPTPSNFQEPFLMEQLPREMQCQFILKPSRLAVTQQLSDSGQKTFKRRRSIINWAFWRGSSIHLDNLPVSPTSPIPGQLFGVSLPDICENDNLPKAVLDMLLFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGAEVQLDCESIFVIASVLKDFLRNIPGSIFSSDLYDHWVCVMDQGNDEEKINAVQRLLDQLPRANVLLLRYLFGLLHNIEQHSSSNQMTAFNLAVCIAPSILWPPTSSSPELENEFTKKVSLLIQFLIENCCRIFGEEITSLLGEVSVRCDTRENASDISCFQLNDSSYDSLENELNDDVDAPCRDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLGHSKDEDIQMGQPLESKPVDTAVYTKVPRAQAASGASTPSRLSTAAADAPKGLRRHRRCSEPSIDYLDSKLSYLRELYQKKLRKSSCDAILSKKDESHLNQNQPLQEGGKTCFKKSLVTGIDSKKNAASKNVKKKSLSDNEGSHVKLFPKSKPVAISVASYSHMSSCDHPRSQPFDMDASEYSPPHTADTLKSSRRQRRCSEPSIDDQHCRLTYLRGIYPKKQQKASYEASLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNVEKVLPPSLNLCSRTSYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQRKQAELSSDFSSSGLISGIPGPSSGQASGRLAYTKKDSVEWHSHMHSVTLHPSTWLKSGVASLKNWSLKKKARAPRPEENKVGSLKGPTDPPAHASGVPEAITLQERQKDVPQRAAEGLGAVQTARWYSSYPSQDSEKYCSSLFSLVEDRLGLHMKSPEEGKDSGQCSPGMLPQRRSSPGSLTVDDASSPDSGPPVVCDAEDRHLAKDM

Target ORF information:

RefSeq Version	NM_001206733.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 20 (ARHGAP20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206733.2

ORF Insert Sequence:

ATGGAAGCGA TGTCCCCCCA GCAGGAGACT CTAGGGGGAC AGCCGGGGCG CTCCTCTTCC 
CTGACAGGAG TGTCTCGGAT CGCAGGCGGC CCCGGCACGA AGAAGAAAAT GAAAACATTA 
GCAGAAAGGA GGAGGAGTGC TCCATCTCTT ATCCTGGATA AAGCCCTGCA AAAGCGGCCC 
AGTACCAAAG AAAGTCCTTC TGCTAGCCTG GACACATGTG CATTTCTGTC ATCATTTGTG 
TGCTCCAGTA GGACTCTGCT TATTGATGGC CGTGTAGAAC TCAAGAGAGG CCTGCAGAGG 
CAGGAGCGAC ATCTTTTCCT GTTCAATGAT CTGTTTGTTG TGGCCAAAAT CAAATATAAC 
AATAACTTTA AGATAAAAAA TAAAATAAAA TTAAGTGACA TGTGGACAGC AAGCTGTGTG 
GATGAAGTGG GAGAAGGCAA CACCAATGCC ACGAAATCCT TTGTCTTGGG CTGGCCCACA 
GTGAACTTCG TGGCCACTTT CAGTTCTCCA GAACAGAAGG ACAAATGGCT CTCTCTCCTT 
CAAAGATACA TCAATCTAGA GAAAGAAAAG GACTACCCGA AGAGCATTCC CCTCAAAATC 
TTCGCCAAGG ACATTGGGAA TTGTGCCTAT TCTAAAACTA TAACAGTAAC GAATTCAGAT 
ACAGCAAATG AAGTTATCAA CATGTCACTA CCAATGCTAG GGATAACTGG CTCTGAGAAA 
GAGTACCAGT TATGGGTCAA TTCTGGCAAA GAAGAGGCAC CATACCCACT TATTGGGCAT 
GAATATCCCT ATGGAATTAA AATGAGCCAT CTTCGAGACA CTGCACTCCT GACACAGGGA 
TCAAAGGACT CCCCCACACC TTCCAACTTC CAAGAGCCCT TCCTCATGGA GCAGCTGCCC 
CGAGAGATGC AGTGTCAGTT CATCCTGAAG CCCAGCCGCC TGGCTGTGAC CCAGCAGCTG 
AGTGATTCAG GTCAGAAGAC ATTTAAAAGG AGAAGATCTA TCATAAACTG GGCCTTTTGG 
CGGGGTTCTA GCATTCACCT GGATAACTTG CCTGTGTCAC CAACGTCTCC TATCCCAGGA 
CAGCTCTTCG GGGTTTCTCT GCCAGATATT TGTGAGAATG ACAATCTGCC CAAAGCTGTC 
TTGGATATGC TTTTATTTCT TAATCAGAAA GGACCTCTCA CAAAAGGCAT CTTCAGACAG 
TCGGCCAATG TGAAATCCTG CAGAGAGCTG AAAGAAAAAC TGAATTCCGG AGCTGAAGTA 
CAACTAGACT GTGAATCTAT TTTCGTAATA GCATCTGTCT TAAAGGATTT TCTACGAAAT 
ATTCCAGGAA GTATTTTTTC ATCAGATCTC TATGATCACT GGGTCTGTGT AATGGATCAA 
GGAAATGATG AAGAGAAAAT AAATGCAGTT CAAAGGCTGT TAGACCAGCT TCCGAGAGCC 
AATGTTCTTC TCCTAAGGTA TCTTTTTGGG TTATTACACA ACATTGAGCA ACATTCTTCA 
TCCAATCAGA TGACCGCATT TAATTTAGCA GTGTGCATCG CCCCAAGCAT TCTTTGGCCT 
CCTACTTCCT CTAGCCCAGA ACTAGAAAAC GAATTTACAA AAAAGGTTTC TCTGCTTATA 
CAGTTTCTTA TTGAAAACTG CTGTCGGATA TTTGGAGAAG AAATCACTTC CCTCTTGGGA 
GAGGTTTCAG TGAGATGTGA TACCAGAGAG AATGCCTCAG ATATCTCTTG CTTCCAGCTG 
AATGACTCCT CCTATGACAG CTTGGAAAAT GAGCTGAATG ACGATGTTGA CGCCCCATGT 
CGTGACTTGG TAAAGAAACT TGGTCAGGGT AGCAGAAGCA TGGACTCCGT GTTAACCCTC 
AGTGACTATG ACCTTGACCA GCCTGAGGTG GAAGGCCTTT TAACCCTGAG TGACTTTGAC 
TTAGGCCATT CTAAAGATGA AGACATTCAA ATGGGACAGC CCCTTGAGTC CAAGCCAGTG 
GACACTGCAG TGTACACCAA GGTCCCACGC GCCCAGGCCG CCTCTGGCGC GAGCACACCC 
AGCCGCCTGT CCACAGCTGC CGCAGATGCT CCAAAAGGCC TGAGGCGACA CCGCCGCTGC 
TCGGAGCCCA GCATCGACTA TCTCGATTCA AAGCTTTCCT ATCTCAGGGA GTTGTATCAG 
AAAAAGCTAC GCAAGTCCAG CTGTGATGCC ATTCTCTCAA AAAAAGATGA GAGCCATCTG 
AATCAGAATC AACCCCTTCA GGAAGGGGGT AAGACATGTT TTAAAAAGAG TTTAGTCACA 
GGCATTGATA GCAAGAAAAA TGCCGCTAGT AAAAATGTTA AGAAGAAAAG TTTGTCTGAT 
AATGAAGGAA GTCATGTAAA ACTTTTTCCT AAATCCAAGC CAGTAGCCAT TTCCGTGGCA 
TCGTACAGTC ACATGTCCTC CTGTGATCAT CCCAGGAGCC AGCCCTTTGA CATGGATGCA 
TCCGAATACT CCCCGCCACA CACTGCAGAC ACCCTCAAGA GTTCAAGGAG GCAACGGCGC 
TGCTCAGAGC CCAGCATTGA TGACCAGCAC TGCAGACTGA CCTATCTCAG GGGGATTTAT 
CCAAAGAAGC AGCAGAAAGC CAGCTACGAA GCCAGTCTCT TGCATGGAGA GGAAGATTAT 
CTCAAGCGGC ACAAGTCTTT ACAAATGGAG GGCCAAAAGC TTATTAATCA GAGTTTGGTC 
ATGGGGATTG AGGTGGGCAA GAGTAGTGCC ACAAACCAAA ACGTGGAGAA GGTTTTACCC 
CCAAGTTTAA ACCTTTGCTC AAGGACTAGC TATTCCAGCT TGTCCTCCCC AGGCACTTCC 
CCATCTGGTT CATCAGTAAG CTCCCAAGAT AGTGCTTTTT CTCAGATTTC TGAACACTCT 
GTGTTTACAC CCACTGAAAC ATCCTCTCCA ATAGATTGTA CTTTTCAAAG AAAACAGGCA 
GAGCTTTCTT CTGACTTTAG CAGTTCTGGC CTCATTTCTG GAATTCCTGG TCCCTCATCA 
GGGCAGGCCA GCGGCCGCCT GGCCTATACA AAAAAGGACA GCGTAGAGTG GCATTCACAC 
ATGCATTCTG TAACTCTTCA CCCCAGCACA TGGTTGAAGA GTGGTGTAGC CAGTTTGAAA 
AACTGGTCTC TCAAAAAGAA GGCAAGGGCA CCCAGACCAG AGGAAAATAA AGTGGGTTCT 
CTAAAAGGAC CCACGGATCC TCCTGCACAT GCTTCTGGTG TTCCGGAAGC CATCACACTA 
CAAGAAAGGC AGAAAGACGT GCCCCAAAGG GCAGCTGAAG GACTGGGAGC TGTTCAGACG 
GCCCGCTGGT ATAGTTCCTA CCCCAGCCAG GACTCAGAGA AATACTGTAG CTCTCTGTTC 
AGCCTCGTGG AAGACAGACT TGGGCTTCAT ATGAAGTCGC CCGAGGAAGG AAAGGATAGC 
GGGCAGTGCT CTCCTGGTAT GCTGCCCCAG AGAAGATCCT CCCCCGGCTC TTTGACTGTG 
GATGATGCGT CCAGCCCAGA CTCAGGGCCG CCCGTGGTGT GTGATGCTGA GGATAGACAT 
TTGGCCAAAG ACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP20 ( NM_001206733.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193662.1 Bos taurus Rho GTPase activating protein 20 (ARHGAP20), mRNA.

Price & Availability↑

CloneID	OBa36039
Clone ID Related Accession (Same CDS sequence)	NM_001206733.1
Accession Version	NM_001206733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 20
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from DAAA02040254.1 and DAAA02040253.1. On or before May 5, 2011 this sequence version replaced XM_593534.4, XM_002692973.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN04019650 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGCGA TGTCCCCCCA GCAGGAGACT GTAGGGGGAC AGCCGGGGCG CTCCTCTTCC 
CTGACAGGAG TGTCTCGGTT CGCCCCCCCC CCCGGCACGA AGAAGAAAAT GAAAACATTA 
GCAGAAAGGA GGAGGAGTGC TCCATCTCTT ATCCTGGATA AAGCCCTGCA AAAGCGGCCC 
AGTACCAAAG AAAGTCCTTC TGCTAGCCTG GACACATGTG CATTTCTGTC ATCATTTGTG 
TGCTCCAGTA GGACTCTGCT TATTGATGGC CGTGTAGAAC TCAAGAGAGG CCTGCAGAGG 
CAGGAGCGAC ATCTTTTCCT GTTCAATGAT CTGTTTGTTG TGGCCAAAAT CAAATATAAC 
AATAACTTTA AGATAAAAAA TAAAATAAAA TTAAGTGACA TGTGGACAGC AAGCTGTGTG 
GATGAAGTGG GAGAAGGCAA CACCAATGCC ACGAAATCCT TTGTCTTGGG CTGGCCCACA 
GTGAACTTCG TGGCCACTTT CAGTTCTCCA GAACAGAAGG ACAAATGGCT CTCTCTCCTT 
CAAAGATACA TCAATCTAGA GAAAGAAAAG GACTACCCGA AGAGCATTCC CCTCAAAATC 
TTCGCCAAGG ACATTGGGAA TTGTGCCTAT TCTAAAACTA TAACAGTAAC GAATTCAGAT 
ACAGCAAATG AAGTTATCAA CATGTCACTA CCAATGCTAG GGATAACTGG CTCTGAGAAA 
GAGTACCAGT TATGGGTCAA TTCTGGCAAA GAAGAGGCAC CATACCCACT TATTGGGCAT 
GAATATCCCT ATGGAATTAA AATGAGCCAT CTTCGAGACA CTGCACTCCT GACACAGGGA 
TCAAAGGACT CCCCCACACC TTCCAACTTC CAAGAGCCCT TCCTCATGGA GCAGCTGCCC 
CGAGAGATGC AGTGTCAGTT CATCCTGAAG CCCAGCCGCC TGGCTGTGAC CCAGCAGCTG 
AGTGATTCAG GTCAGAAGAC ATTTAAAAGG AGAAGATCTA TCATAAACTG GGCCTTTTGG 
CGGGGTTCTA GCATTCACCT GGATAACTTG CCTGTGTCAC CAACGTCTCC TATCCCAGGA 
CAGCTCTTCG GGGTTTCTCT GCCAGATATT TGTGAGAATG ACAATCTGCC CAAAGCTGTC 
TTGGATATGC TTTTATTTCT TAATCAGAAA GGACCTCTCA CAAAAGGCAT CTTCAGACAG 
TCGGCCAATG TGAAATCCTG CAGAGAGCTG AAAGAAAAAC TGAATTCCGG AGCTGAAGTA 
CAACTAGACT GTGAATCTAT TTTCGTAATA GCATCTGTCT TAAAGGATTT TCTACGAAAT 
ATTCCAGGAA GTATTTTTTC ATCAGATCTC TATGATCACT GGGTCTGTGT AATGGATCAA 
GGAAATGATG AAGAGAAAAT AAATGCAGTT CAAAGGCTGT TAGACCAGCT TCCGAGAGCC 
AATGTTCTTC TCCTAAGGTA TCTTTTTGGG TTATTACACA ACATTGAGCA ACATTCTTCA 
TCCAATCAGA TGACCGCATT TAATTTAGCA GTGTGCATCG CCCCAAGCAT TCTTTGGCCT 
CCTACTTCCT CTAGCCCAGA ACTAGAAAAC GAATTTACAA AAAAGGTTTC TCTGCTTATA 
CAGTTTCTTA TTGAAAACTG CTGTCGGATA TTTGGAGAAG AAATCACTTC CCTCTTGGGA 
GAGGTTTCAG TGAGATGTGA TACCAGAGAG AATGCCTCAG ATATCTCTTG CTTCCAGCTG 
AATGACTCCT CCTATGACAG CTTGGAAAAT GAGCTGAATG ACGATGTTGA CGCCCCATGT 
CGTGACTTGG TAAAGAAACT TGGTCAGGGT AGCAGAAGCA TGGACTCCGT GTTAACCCTC 
AGTGACTATG ACCTTGACCA GCCTGAGGTG GAAGGCCTTT TAACCCTGAG TGACTTTGAC 
TTAGGCCATT CTAAAGATGA AGACATTCAA ATGGGACAGC CCCTTGAGTC CAAGCCAGTG 
GACACTGCAG TGTACACCAA GGTCCCACGC GCCCAGGCCG CCTCTGGCGC GAGCACACCC 
AGCCGCCTGT CCACAGCTGC CGCAGATGCT CCAAAAGGCC TGAGGCGACA CCGCCGCTGC 
TCGGAGCCCA GCATCGACTA TCTCGATTCA AAGCTTTCCT ATCTCAGGGA GTTGTATCAG 
AAAAAGCTAC GCAAGTCCAG CTGTGATGCC ATTCTCTCAA AAAAAGATGA GAGCCATCTG 
AATCAGAATC AACCCCTTCA GGAAGGGGGT AAGACATGTT TTAAAAAGAG TTTAGTCACA 
GGCATTGATA GCAAGAAAAA TGCCGCTAGT AAAAATGTTA AGAAGAAAAG TTTGTCTGAT 
AATGAAGGAA GTCATGTAAA ACTTTTTCCT AAATCCAAGC CAGTAGCCAT TTCCGTGGCA 
TCGTACAGTC ACATGTCCTC CTGTGATCAT CCCAGGAGCC AGCCCTTTGA CATGGATGCA 
TCCGAATACT CCCCGCCACA CACTGCAGAC ACCCTCAAGA GTTCAAGGAG GCAACGGCGC 
TGCTCAGAGC CCAGCATTGA TGACCAGCAC TGCAGACTGA CCTATCTCAG GGGGATTTAT 
CCAAAGAAGC AGCAGAAAGC CAGCTACGAA GCCAGTCTCT TGCATGGAGA GGAAGATTAT 
CTCAAGCGGC ACAAGTCTTT ACAAATGGAG GGCCAAAAGC TTATTAATCA GAGTTTGGTC 
ATGGGGATTG AGGTGGGCAA GAGTAGTGCC ACAAACCAAA ACGTGGAGAA GGTTTTACCC 
CCAAGTTTAA ACCTTTGCTC AAGGACTAGC TATTCCAGCT TGTCCTCCCC AGGCACTTCC 
CCATCTGGTT CATCAGTAAG CTCCCAAGAT AGTGCTTTTT CTCAGATTTC TGAACACTCT 
GTGTTTACAC CCACTGAAAC ATCCTCTCCA ATAGATTGTA CTTTTCAAAG AAAACAGGCA 
GAGCTTTCTT CTGACTTTAG CAGTTCTGGC CTCATTTCTG GAATTCCTGG TCCCTCATCA 
GGGCAGGCCA GCGGCCGCCT GGCCTATACA AAAAAGGACA GCGTAGAGTG GCATTCACAC 
ATGCATTCTG TAACTCTTCA CCCCAGCACA TGGTTGAAGA GTGGTGTAGC CAGTTTGAAA 
AACTGGTCTC TCAAAAAGAA GGCAAGGGCA CCCAGACCAG AGGAAAATAA AGTGGGTTCT 
CTAAAAGGAC CCACGGATCC TCCTGCACAT GCTTCTGGTG TTCCGGAAGC CATCACACTA 
CAAGAAAGGC AGAAAGACGT GCCCCAAAGG GCAGCTGAAG GACTGGGAGC TGTTCAGACG 
GCCCGCTGGT ATAGTTCCTA CCCCAGCCAG GACTCAGAGA AATACTGTAG CTCTCTGTTC 
AGCCTCGTGG AAGACAGACT TGGGCTTCAT ATGAAGTCGC CCGAGGAAGG AAAGGATAGC 
GGGCAGTGCT CTCCTGGTAT GCTGCCCCAG AGAAGATCCT CCCCCGGCTC TTTGACTGTG 
GATGATGCGT CCAGCCCAGA CTCAGGGCCG CCCGTGGTGT GTGATGCTGA GGATAGACAT 
TTGGCCAAAG ACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193662.1
CDS	1..3558
Translation	MEAMSPQQETVGGQPGRSSSLTGVSRFAPPPGTKKKMKTLAERRRSAPSLILDKALQKRPSTKESPSASLDTCAFLSSFVCSSRTLLIDGRVELKRGLQRQERHLFLFNDLFVVAKIKYNNNFKIKNKIKLSDMWTASCVDEVGEGNTNATKSFVLGWPTVNFVATFSSPEQKDKWLSLLQRYINLEKEKDYPKSIPLKIFAKDIGNCAYSKTITVTNSDTANEVINMSLPMLGITGSEKEYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDTALLTQGSKDSPTPSNFQEPFLMEQLPREMQCQFILKPSRLAVTQQLSDSGQKTFKRRRSIINWAFWRGSSIHLDNLPVSPTSPIPGQLFGVSLPDICENDNLPKAVLDMLLFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGAEVQLDCESIFVIASVLKDFLRNIPGSIFSSDLYDHWVCVMDQGNDEEKINAVQRLLDQLPRANVLLLRYLFGLLHNIEQHSSSNQMTAFNLAVCIAPSILWPPTSSSPELENEFTKKVSLLIQFLIENCCRIFGEEITSLLGEVSVRCDTRENASDISCFQLNDSSYDSLENELNDDVDAPCRDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLGHSKDEDIQMGQPLESKPVDTAVYTKVPRAQAASGASTPSRLSTAAADAPKGLRRHRRCSEPSIDYLDSKLSYLRELYQKKLRKSSCDAILSKKDESHLNQNQPLQEGGKTCFKKSLVTGIDSKKNAASKNVKKKSLSDNEGSHVKLFPKSKPVAISVASYSHMSSCDHPRSQPFDMDASEYSPPHTADTLKSSRRQRRCSEPSIDDQHCRLTYLRGIYPKKQQKASYEASLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNVEKVLPPSLNLCSRTSYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQRKQAELSSDFSSSGLISGIPGPSSGQASGRLAYTKKDSVEWHSHMHSVTLHPSTWLKSGVASLKNWSLKKKARAPRPEENKVGSLKGPTDPPAHASGVPEAITLQERQKDVPQRAAEGLGAVQTARWYSSYPSQDSEKYCSSLFSLVEDRLGLHMKSPEEGKDSGQCSPGMLPQRRSSPGSLTVDDASSPDSGPPVVCDAEDRHLAKDM

Target ORF information:

RefSeq Version	NM_001206733.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 20 (ARHGAP20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206733.1

ORF Insert Sequence:

ATGGAAGCGA TGTCCCCCCA GCAGGAGACT GTAGGGGGAC AGCCGGGGCG CTCCTCTTCC 
CTGACAGGAG TGTCTCGGTT CGCCCCCCCC CCCGGCACGA AGAAGAAAAT GAAAACATTA 
GCAGAAAGGA GGAGGAGTGC TCCATCTCTT ATCCTGGATA AAGCCCTGCA AAAGCGGCCC 
AGTACCAAAG AAAGTCCTTC TGCTAGCCTG GACACATGTG CATTTCTGTC ATCATTTGTG 
TGCTCCAGTA GGACTCTGCT TATTGATGGC CGTGTAGAAC TCAAGAGAGG CCTGCAGAGG 
CAGGAGCGAC ATCTTTTCCT GTTCAATGAT CTGTTTGTTG TGGCCAAAAT CAAATATAAC 
AATAACTTTA AGATAAAAAA TAAAATAAAA TTAAGTGACA TGTGGACAGC AAGCTGTGTG 
GATGAAGTGG GAGAAGGCAA CACCAATGCC ACGAAATCCT TTGTCTTGGG CTGGCCCACA 
GTGAACTTCG TGGCCACTTT CAGTTCTCCA GAACAGAAGG ACAAATGGCT CTCTCTCCTT 
CAAAGATACA TCAATCTAGA GAAAGAAAAG GACTACCCGA AGAGCATTCC CCTCAAAATC 
TTCGCCAAGG ACATTGGGAA TTGTGCCTAT TCTAAAACTA TAACAGTAAC GAATTCAGAT 
ACAGCAAATG AAGTTATCAA CATGTCACTA CCAATGCTAG GGATAACTGG CTCTGAGAAA 
GAGTACCAGT TATGGGTCAA TTCTGGCAAA GAAGAGGCAC CATACCCACT TATTGGGCAT 
GAATATCCCT ATGGAATTAA AATGAGCCAT CTTCGAGACA CTGCACTCCT GACACAGGGA 
TCAAAGGACT CCCCCACACC TTCCAACTTC CAAGAGCCCT TCCTCATGGA GCAGCTGCCC 
CGAGAGATGC AGTGTCAGTT CATCCTGAAG CCCAGCCGCC TGGCTGTGAC CCAGCAGCTG 
AGTGATTCAG GTCAGAAGAC ATTTAAAAGG AGAAGATCTA TCATAAACTG GGCCTTTTGG 
CGGGGTTCTA GCATTCACCT GGATAACTTG CCTGTGTCAC CAACGTCTCC TATCCCAGGA 
CAGCTCTTCG GGGTTTCTCT GCCAGATATT TGTGAGAATG ACAATCTGCC CAAAGCTGTC 
TTGGATATGC TTTTATTTCT TAATCAGAAA GGACCTCTCA CAAAAGGCAT CTTCAGACAG 
TCGGCCAATG TGAAATCCTG CAGAGAGCTG AAAGAAAAAC TGAATTCCGG AGCTGAAGTA 
CAACTAGACT GTGAATCTAT TTTCGTAATA GCATCTGTCT TAAAGGATTT TCTACGAAAT 
ATTCCAGGAA GTATTTTTTC ATCAGATCTC TATGATCACT GGGTCTGTGT AATGGATCAA 
GGAAATGATG AAGAGAAAAT AAATGCAGTT CAAAGGCTGT TAGACCAGCT TCCGAGAGCC 
AATGTTCTTC TCCTAAGGTA TCTTTTTGGG TTATTACACA ACATTGAGCA ACATTCTTCA 
TCCAATCAGA TGACCGCATT TAATTTAGCA GTGTGCATCG CCCCAAGCAT TCTTTGGCCT 
CCTACTTCCT CTAGCCCAGA ACTAGAAAAC GAATTTACAA AAAAGGTTTC TCTGCTTATA 
CAGTTTCTTA TTGAAAACTG CTGTCGGATA TTTGGAGAAG AAATCACTTC CCTCTTGGGA 
GAGGTTTCAG TGAGATGTGA TACCAGAGAG AATGCCTCAG ATATCTCTTG CTTCCAGCTG 
AATGACTCCT CCTATGACAG CTTGGAAAAT GAGCTGAATG ACGATGTTGA CGCCCCATGT 
CGTGACTTGG TAAAGAAACT TGGTCAGGGT AGCAGAAGCA TGGACTCCGT GTTAACCCTC 
AGTGACTATG ACCTTGACCA GCCTGAGGTG GAAGGCCTTT TAACCCTGAG TGACTTTGAC 
TTAGGCCATT CTAAAGATGA AGACATTCAA ATGGGACAGC CCCTTGAGTC CAAGCCAGTG 
GACACTGCAG TGTACACCAA GGTCCCACGC GCCCAGGCCG CCTCTGGCGC GAGCACACCC 
AGCCGCCTGT CCACAGCTGC CGCAGATGCT CCAAAAGGCC TGAGGCGACA CCGCCGCTGC 
TCGGAGCCCA GCATCGACTA TCTCGATTCA AAGCTTTCCT ATCTCAGGGA GTTGTATCAG 
AAAAAGCTAC GCAAGTCCAG CTGTGATGCC ATTCTCTCAA AAAAAGATGA GAGCCATCTG 
AATCAGAATC AACCCCTTCA GGAAGGGGGT AAGACATGTT TTAAAAAGAG TTTAGTCACA 
GGCATTGATA GCAAGAAAAA TGCCGCTAGT AAAAATGTTA AGAAGAAAAG TTTGTCTGAT 
AATGAAGGAA GTCATGTAAA ACTTTTTCCT AAATCCAAGC CAGTAGCCAT TTCCGTGGCA 
TCGTACAGTC ACATGTCCTC CTGTGATCAT CCCAGGAGCC AGCCCTTTGA CATGGATGCA 
TCCGAATACT CCCCGCCACA CACTGCAGAC ACCCTCAAGA GTTCAAGGAG GCAACGGCGC 
TGCTCAGAGC CCAGCATTGA TGACCAGCAC TGCAGACTGA CCTATCTCAG GGGGATTTAT 
CCAAAGAAGC AGCAGAAAGC CAGCTACGAA GCCAGTCTCT TGCATGGAGA GGAAGATTAT 
CTCAAGCGGC ACAAGTCTTT ACAAATGGAG GGCCAAAAGC TTATTAATCA GAGTTTGGTC 
ATGGGGATTG AGGTGGGCAA GAGTAGTGCC ACAAACCAAA ACGTGGAGAA GGTTTTACCC 
CCAAGTTTAA ACCTTTGCTC AAGGACTAGC TATTCCAGCT TGTCCTCCCC AGGCACTTCC 
CCATCTGGTT CATCAGTAAG CTCCCAAGAT AGTGCTTTTT CTCAGATTTC TGAACACTCT 
GTGTTTACAC CCACTGAAAC ATCCTCTCCA ATAGATTGTA CTTTTCAAAG AAAACAGGCA 
GAGCTTTCTT CTGACTTTAG CAGTTCTGGC CTCATTTCTG GAATTCCTGG TCCCTCATCA 
GGGCAGGCCA GCGGCCGCCT GGCCTATACA AAAAAGGACA GCGTAGAGTG GCATTCACAC 
ATGCATTCTG TAACTCTTCA CCCCAGCACA TGGTTGAAGA GTGGTGTAGC CAGTTTGAAA 
AACTGGTCTC TCAAAAAGAA GGCAAGGGCA CCCAGACCAG AGGAAAATAA AGTGGGTTCT 
CTAAAAGGAC CCACGGATCC TCCTGCACAT GCTTCTGGTG TTCCGGAAGC CATCACACTA 
CAAGAAAGGC AGAAAGACGT GCCCCAAAGG GCAGCTGAAG GACTGGGAGC TGTTCAGACG 
GCCCGCTGGT ATAGTTCCTA CCCCAGCCAG GACTCAGAGA AATACTGTAG CTCTCTGTTC 
AGCCTCGTGG AAGACAGACT TGGGCTTCAT ATGAAGTCGC CCGAGGAAGG AAAGGATAGC 
GGGCAGTGCT CTCCTGGTAT GCTGCCCCAG AGAAGATCCT CCCCCGGCTC TTTGACTGTG 
GATGATGCGT CCAGCCCAGA CTCAGGGCCG CCCGTGGTGT GTGATGCTGA GGATAGACAT 
TTGGCCAAAG ACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP20 ( XM_005215783.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215840.1 Bos taurus Rho GTPase activating protein 20 (ARHGAP20), transcript variant X2, mRNA.

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CloneID	OBa26195
Clone ID Related Accession (Same CDS sequence)	XM_005215783.4 , XM_005215783.3 , XM_024975249.1
Accession Version	XM_005215783.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215783.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACAT TAGCAGAAAG GAGGAGGAGT GCTCCATCTC TTATCCTGGA TAAAGCCCTG 
CAAAAGCGGC CCAGTACCAA AGAAAGTCCT TCTGCTAGCC TGGACACATG TGCATTTCTG 
TCATCATTTG TGTGCTCCAG TAGGACTCTG CTTATTGATG GCCGTGTAGA ACTCAAGAGA 
GGCCTGCAGA GGCAGGAGCG ACATCTTTTC CTGTTCAATG ATCTGTTTGT TGTGGCCAAA 
ATCAAATATA ACAATAACTT TAAGATAAAA AATAAAATAA AATTAAGTGA CATGTGGACA 
GCAAGCTGTG TGGATGAAGT GGGAGAAGGC AACACCAATG CCACGAAATC CTTTGTCTTG 
GGCTGGCCCA CAGTGAACTT CGTGGCCACT TTCAGTTCTC CAGAACAGAA GGACAAATGG 
CTCTCTCTCC TTCAAAGATA CATCAATCTA GAGAAAGAAA AGGACTACCC GAAGAGCATT 
CCCCTCAAAA TCTTCGCCAA GGACATTGGG AATTGTGCCT ATTCTAAAAC TATAACAGTA 
ACGAATTCAG ATACAGCAAA TGAAGTTATC AACATGTCAC TACCAATGCT AGGGATAACT 
GGCTCTGAGA AAGAGTACCA GTTATGGGTC AATTCTGGCA AAGAAGAGGC ACCATACCCA 
CTTATTGGGC ATGAATATCC CTATGGAATT AAAATGAGCC ATCTTCGAGA CACTGCACTC 
CTGACACAGG GATCAAAGGA CTCCCCCACA CCTTCCAACT TCCAAGAGCC CTTCCTCATG 
GAGCAGCTGC CCCGAGAGAT GCAGTGTCAG TTCATCCTGA AGCCCAGCCG CCTGGCTGTG 
ACCCAGCAGC TGAGTGATTC AGGTCAGAAG ACATTTAAAA GGAGAAGATC TATCATAAAC 
TGGGCCTTTT GGCGGGGTTC TAGCATTCAC CTGGATAACT TGCCTGTGTC ACCAACGTCT 
CCTATCCCAG GACAGCTCTT CGGGGTTTCT CTGCCAGATA TTTGTGAGAA TGACAATCTG 
CCCAAAGCTG TCTTGGATAT GCTTTTATTT CTTAATCAGA AAGGACCTCT CACAAAAGGC 
ATCTTCAGAC AGTCGGCCAA TGTGAAATCC TGCAGAGAGC TGAAAGAAAA ACTGAATTCC 
GGAGCTGAAG TACAACTAGA CTGTGAATCT ATTTTCGTAA TAGCATCTGT CTTAAAGGAT 
TTTCTACGAA ATATTCCAGG AAGTATTTTT TCATCAGATC TCTATGATCA CTGGGTCTGT 
GTAATGGATC AAGGAAATGA TGAAGAGAAA ATAAATGCAG TTCAAAGGCT GTTAGACCAG 
CTTCCGAGAG CCAATGTTCT TCTCCTAAGG TATCTTTTTG GGTTATTACA CAACATTGAG 
CAACATTCTT CATCCAATCA GATGACCGCA TTTAATTTAG CAGTGTGCAT CGCCCCAAGC 
ATTCTTTGGC CTCCTACTTC CTCTAGCCCA GAACTAGAAA ACGAATTTAC AAAAAAGGTT 
TCTCTGCTTA TACAGTTTCT TATTGAAAAC TGCTGTCGGA TATTTGGAGA AGAAATCACT 
TCCCTCTTGG GAGAGGTTTC AGTGAGATGT GATACCAGAG AGAATGCCTC AGATATCTCT 
TGCTTCCAGC TGAATGACTC CTCCTATGAC AGCTTGGAAA ATGAGCTGAA TGACGATGTT 
GACGCCCCAT GTCGTGACTT GGTAAAGAAA CTTGGTCAGG GTAGCAGAAG CATGGACTCC 
GTGTTAACCC TCAGTGACTA TGACCTTGAC CAGCCTGAGG TGGAAGGCCT TTTAACCCTG 
AGTGACTTTG ACTTAGGCCA TTCTAAAGAT GAAGACATTC AAATGGGACA GCCCCTTGAG 
TCCAAGCCAG TGGACACTGC AGTGTACACC AAGGTCCCAC GCGCCCAGGC CGCCTCTGGC 
GCGAGCACAC CCAGCCGCCT GTCCACAGCT GCCGCAGATG CTCCAAAAGG CCTGAGGCGA 
CACCGCCGCT GCTCGGAGCC CAGCATCGAC TATCTCGATT CAAAGCTTTC CTATCTCAGG 
GAGTTGTATC AGAAAAAGCT ACGCAAGTCC AGCTGTGATG CCATTCTCTC AAAAAAAGAT 
GAGAGCCATC TGAATCAGAA TCAACCCCTT CAGGAAGGGG GTAAGACATG TTTTAAAAAG 
AGTTTAGTCA CAGGCATTGA TAGCAAGAAA AATGCCGCTA GTAAAAATGT TAAGAAGAAA 
AGTTTGTCTG ATAATGAAGG AAGTCATGTA AAACTTTTTC CTAAATCCAA GCCAGTAGCC 
ATTTCCGTGG CATCGTACAG TCACATGTCC TCCTGTGATC ATCCCAGGAG CCAGCCCTTT 
GACATGGATG CATCCGAATA CTCCCCGCCA CACACTGCAG ACACCCTCAA GAGTTCAAGG 
AGGCAACGGC GCTGCTCAGA GCCCAGCATT GATGACCAGC ACTGCAGACT GACCTATCTC 
AGGGGGATTT ATCCAAAGAA GCAGCAGAAA GCCAGCTACG AAGCCAGTCT CTTGCATGGA 
GAGGAAGATT ATCTCAAGCG GCACAAGTCT TTACAAATGG AGGGCCAAAA GCTTATTAAT 
CAGAGTTTGG TCATGGGGAT TGAGGTGGGC AAGAGTAGTG CCACAAACCA AAACGTGGAG 
AAGGTTTTAC CCCCAAGTTT AAACCTTTGC TCAAGGACTA GCTATTCCAG CTTGTCCTCC 
CCAGGCACTT CCCCATCTGG TTCATCAGTA AGCTCCCAAG ATAGTGCTTT TTCTCAGATT 
TCTGAACACT CTGTGTTTAC ACCCACTGAA ACATCCTCTC CAATAGATTG TACTTTTCAA 
AGAAAACAGG CAGAGCTTTC TTCTGACTTT AGCAGTTCTG GCCTCATTTC TGGAATTCCT 
GGTCCCTCAT CAGGGCAGGC CAGCGGCCGC CTGGCCTATA CAAAAAAGGA CAGCGTAGAG 
TGGCATTCAC ACATGCATTC TGTAACTCTT CACCCCAGCA CATGGTTGAA GAGTGGTGTA 
GCCAGTTTGA AAAACTGGTC TCTCAAAAAG AAGGCAAGGG CACCCAGACC AGAGGAAAAT 
AAAGTGGGTT CTCTAAAAGG ACCCACGGAT CCTCCTGCAC ATGCTTCTGG TGTTCCGGAA 
GCCATCACAC TACAAGAAAG GCAGAAAGAC GTGCCCCAAA GGGCAGCTGA AGGACTGGGA 
GCTGTTCAGA CGGCCCGCTG GTATAGTTCC TACCCCAGCC AGGACTCAGA GAAATACTGT 
AGCTCTCTGT TCAGCCTCGT GGAAGACAGA CTTGGGCTTC ATATGAAGTC GCCCGAGGAA 
GGAAAGGATA GCGGGCAGTG CTCTCCTGGT ATGCTGCCCC AGAGAAGATC CTCCCCCGGC 
TCTTTGACTG TGGATGATGC GTCCAGCCCA GACTCAGGGC CGCCCGTGGT GTGTGATGCT 
GAGGATAGAC ATTTGGCCAA AGACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215840.1
CDS	133..3582
Translation	MKTLAERRRSAPSLILDKALQKRPSTKESPSASLDTCAFLSSFVCSSRTLLIDGRVELKRGLQRQERHLFLFNDLFVVAKIKYNNNFKIKNKIKLSDMWTASCVDEVGEGNTNATKSFVLGWPTVNFVATFSSPEQKDKWLSLLQRYINLEKEKDYPKSIPLKIFAKDIGNCAYSKTITVTNSDTANEVINMSLPMLGITGSEKEYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDTALLTQGSKDSPTPSNFQEPFLMEQLPREMQCQFILKPSRLAVTQQLSDSGQKTFKRRRSIINWAFWRGSSIHLDNLPVSPTSPIPGQLFGVSLPDICENDNLPKAVLDMLLFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGAEVQLDCESIFVIASVLKDFLRNIPGSIFSSDLYDHWVCVMDQGNDEEKINAVQRLLDQLPRANVLLLRYLFGLLHNIEQHSSSNQMTAFNLAVCIAPSILWPPTSSSPELENEFTKKVSLLIQFLIENCCRIFGEEITSLLGEVSVRCDTRENASDISCFQLNDSSYDSLENELNDDVDAPCRDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLGHSKDEDIQMGQPLESKPVDTAVYTKVPRAQAASGASTPSRLSTAAADAPKGLRRHRRCSEPSIDYLDSKLSYLRELYQKKLRKSSCDAILSKKDESHLNQNQPLQEGGKTCFKKSLVTGIDSKKNAASKNVKKKSLSDNEGSHVKLFPKSKPVAISVASYSHMSSCDHPRSQPFDMDASEYSPPHTADTLKSSRRQRRCSEPSIDDQHCRLTYLRGIYPKKQQKASYEASLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNVEKVLPPSLNLCSRTSYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQRKQAELSSDFSSSGLISGIPGPSSGQASGRLAYTKKDSVEWHSHMHSVTLHPSTWLKSGVASLKNWSLKKKARAPRPEENKVGSLKGPTDPPAHASGVPEAITLQERQKDVPQRAAEGLGAVQTARWYSSYPSQDSEKYCSSLFSLVEDRLGLHMKSPEEGKDSGQCSPGMLPQRRSSPGSLTVDDASSPDSGPPVVCDAEDRHLAKDM

Target ORF information:

RefSeq Version	XM_005215783.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 20 (ARHGAP20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215783.4

ORF Insert Sequence:

ATGAAAACAT TAGCAGAAAG GAGGAGGAGT GCTCCATCTC TTATCCTGGA TAAAGCCCTG 
CAAAAGCGGC CCAGTACCAA AGAAAGTCCT TCTGCTAGCC TGGACACATG TGCATTTCTG 
TCATCATTTG TGTGCTCCAG TAGGACTCTG CTTATTGATG GCCGTGTAGA ACTCAAGAGA 
GGCCTGCAGA GGCAGGAGCG ACATCTTTTC CTGTTCAATG ATCTGTTTGT TGTGGCCAAA 
ATCAAATATA ACAATAACTT TAAGATAAAA AATAAAATAA AATTAAGTGA CATGTGGACA 
GCAAGCTGTG TGGATGAAGT GGGAGAAGGC AACACCAATG CCACGAAATC CTTTGTCTTG 
GGCTGGCCCA CAGTGAACTT CGTGGCCACT TTCAGTTCTC CAGAACAGAA GGACAAATGG 
CTCTCTCTCC TTCAAAGATA CATCAATCTA GAGAAAGAAA AGGACTACCC GAAGAGCATT 
CCCCTCAAAA TCTTCGCCAA GGACATTGGG AATTGTGCCT ATTCTAAAAC TATAACAGTA 
ACGAATTCAG ATACAGCAAA TGAAGTTATC AACATGTCAC TACCAATGCT AGGGATAACT 
GGCTCTGAGA AAGAGTACCA GTTATGGGTC AATTCTGGCA AAGAAGAGGC ACCATACCCA 
CTTATTGGGC ATGAATATCC CTATGGAATT AAAATGAGCC ATCTTCGAGA CACTGCACTC 
CTGACACAGG GATCAAAGGA CTCCCCCACA CCTTCCAACT TCCAAGAGCC CTTCCTCATG 
GAGCAGCTGC CCCGAGAGAT GCAGTGTCAG TTCATCCTGA AGCCCAGCCG CCTGGCTGTG 
ACCCAGCAGC TGAGTGATTC AGGTCAGAAG ACATTTAAAA GGAGAAGATC TATCATAAAC 
TGGGCCTTTT GGCGGGGTTC TAGCATTCAC CTGGATAACT TGCCTGTGTC ACCAACGTCT 
CCTATCCCAG GACAGCTCTT CGGGGTTTCT CTGCCAGATA TTTGTGAGAA TGACAATCTG 
CCCAAAGCTG TCTTGGATAT GCTTTTATTT CTTAATCAGA AAGGACCTCT CACAAAAGGC 
ATCTTCAGAC AGTCGGCCAA TGTGAAATCC TGCAGAGAGC TGAAAGAAAA ACTGAATTCC 
GGAGCTGAAG TACAACTAGA CTGTGAATCT ATTTTCGTAA TAGCATCTGT CTTAAAGGAT 
TTTCTACGAA ATATTCCAGG AAGTATTTTT TCATCAGATC TCTATGATCA CTGGGTCTGT 
GTAATGGATC AAGGAAATGA TGAAGAGAAA ATAAATGCAG TTCAAAGGCT GTTAGACCAG 
CTTCCGAGAG CCAATGTTCT TCTCCTAAGG TATCTTTTTG GGTTATTACA CAACATTGAG 
CAACATTCTT CATCCAATCA GATGACCGCA TTTAATTTAG CAGTGTGCAT CGCCCCAAGC 
ATTCTTTGGC CTCCTACTTC CTCTAGCCCA GAACTAGAAA ACGAATTTAC AAAAAAGGTT 
TCTCTGCTTA TACAGTTTCT TATTGAAAAC TGCTGTCGGA TATTTGGAGA AGAAATCACT 
TCCCTCTTGG GAGAGGTTTC AGTGAGATGT GATACCAGAG AGAATGCCTC AGATATCTCT 
TGCTTCCAGC TGAATGACTC CTCCTATGAC AGCTTGGAAA ATGAGCTGAA TGACGATGTT 
GACGCCCCAT GTCGTGACTT GGTAAAGAAA CTTGGTCAGG GTAGCAGAAG CATGGACTCC 
GTGTTAACCC TCAGTGACTA TGACCTTGAC CAGCCTGAGG TGGAAGGCCT TTTAACCCTG 
AGTGACTTTG ACTTAGGCCA TTCTAAAGAT GAAGACATTC AAATGGGACA GCCCCTTGAG 
TCCAAGCCAG TGGACACTGC AGTGTACACC AAGGTCCCAC GCGCCCAGGC CGCCTCTGGC 
GCGAGCACAC CCAGCCGCCT GTCCACAGCT GCCGCAGATG CTCCAAAAGG CCTGAGGCGA 
CACCGCCGCT GCTCGGAGCC CAGCATCGAC TATCTCGATT CAAAGCTTTC CTATCTCAGG 
GAGTTGTATC AGAAAAAGCT ACGCAAGTCC AGCTGTGATG CCATTCTCTC AAAAAAAGAT 
GAGAGCCATC TGAATCAGAA TCAACCCCTT CAGGAAGGGG GTAAGACATG TTTTAAAAAG 
AGTTTAGTCA CAGGCATTGA TAGCAAGAAA AATGCCGCTA GTAAAAATGT TAAGAAGAAA 
AGTTTGTCTG ATAATGAAGG AAGTCATGTA AAACTTTTTC CTAAATCCAA GCCAGTAGCC 
ATTTCCGTGG CATCGTACAG TCACATGTCC TCCTGTGATC ATCCCAGGAG CCAGCCCTTT 
GACATGGATG CATCCGAATA CTCCCCGCCA CACACTGCAG ACACCCTCAA GAGTTCAAGG 
AGGCAACGGC GCTGCTCAGA GCCCAGCATT GATGACCAGC ACTGCAGACT GACCTATCTC 
AGGGGGATTT ATCCAAAGAA GCAGCAGAAA GCCAGCTACG AAGCCAGTCT CTTGCATGGA 
GAGGAAGATT ATCTCAAGCG GCACAAGTCT TTACAAATGG AGGGCCAAAA GCTTATTAAT 
CAGAGTTTGG TCATGGGGAT TGAGGTGGGC AAGAGTAGTG CCACAAACCA AAACGTGGAG 
AAGGTTTTAC CCCCAAGTTT AAACCTTTGC TCAAGGACTA GCTATTCCAG CTTGTCCTCC 
CCAGGCACTT CCCCATCTGG TTCATCAGTA AGCTCCCAAG ATAGTGCTTT TTCTCAGATT 
TCTGAACACT CTGTGTTTAC ACCCACTGAA ACATCCTCTC CAATAGATTG TACTTTTCAA 
AGAAAACAGG CAGAGCTTTC TTCTGACTTT AGCAGTTCTG GCCTCATTTC TGGAATTCCT 
GGTCCCTCAT CAGGGCAGGC CAGCGGCCGC CTGGCCTATA CAAAAAAGGA CAGCGTAGAG 
TGGCATTCAC ACATGCATTC TGTAACTCTT CACCCCAGCA CATGGTTGAA GAGTGGTGTA 
GCCAGTTTGA AAAACTGGTC TCTCAAAAAG AAGGCAAGGG CACCCAGACC AGAGGAAAAT 
AAAGTGGGTT CTCTAAAAGG ACCCACGGAT CCTCCTGCAC ATGCTTCTGG TGTTCCGGAA 
GCCATCACAC TACAAGAAAG GCAGAAAGAC GTGCCCCAAA GGGCAGCTGA AGGACTGGGA 
GCTGTTCAGA CGGCCCGCTG GTATAGTTCC TACCCCAGCC AGGACTCAGA GAAATACTGT 
AGCTCTCTGT TCAGCCTCGT GGAAGACAGA CTTGGGCTTC ATATGAAGTC GCCCGAGGAA 
GGAAAGGATA GCGGGCAGTG CTCTCCTGGT ATGCTGCCCC AGAGAAGATC CTCCCCCGGC 
TCTTTGACTG TGGATGATGC GTCCAGCCCA GACTCAGGGC CGCCCGTGGT GTGTGATGCT 
GAGGATAGAC ATTTGGCCAA AGACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP20 ( XM_005215783.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215840.1 Bos taurus Rho GTPase activating protein 20 (ARHGAP20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa26195
Clone ID Related Accession (Same CDS sequence)	XM_005215783.4 , XM_005215783.3 , XM_024975249.1
Accession Version	XM_005215783.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104407.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215783.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACAT TAGCAGAAAG GAGGAGGAGT GCTCCATCTC TTATCCTGGA TAAAGCCCTG 
CAAAAGCGGC CCAGTACCAA AGAAAGTCCT TCTGCTAGCC TGGACACATG TGCATTTCTG 
TCATCATTTG TGTGCTCCAG TAGGACTCTG CTTATTGATG GCCGTGTAGA ACTCAAGAGA 
GGCCTGCAGA GGCAGGAGCG ACATCTTTTC CTGTTCAATG ATCTGTTTGT TGTGGCCAAA 
ATCAAATATA ACAATAACTT TAAGATAAAA AATAAAATAA AATTAAGTGA CATGTGGACA 
GCAAGCTGTG TGGATGAAGT GGGAGAAGGC AACACCAATG CCACGAAATC CTTTGTCTTG 
GGCTGGCCCA CAGTGAACTT CGTGGCCACT TTCAGTTCTC CAGAACAGAA GGACAAATGG 
CTCTCTCTCC TTCAAAGATA CATCAATCTA GAGAAAGAAA AGGACTACCC GAAGAGCATT 
CCCCTCAAAA TCTTCGCCAA GGACATTGGG AATTGTGCCT ATTCTAAAAC TATAACAGTA 
ACGAATTCAG ATACAGCAAA TGAAGTTATC AACATGTCAC TACCAATGCT AGGGATAACT 
GGCTCTGAGA AAGAGTACCA GTTATGGGTC AATTCTGGCA AAGAAGAGGC ACCATACCCA 
CTTATTGGGC ATGAATATCC CTATGGAATT AAAATGAGCC ATCTTCGAGA CACTGCACTC 
CTGACACAGG GATCAAAGGA CTCCCCCACA CCTTCCAACT TCCAAGAGCC CTTCCTCATG 
GAGCAGCTGC CCCGAGAGAT GCAGTGTCAG TTCATCCTGA AGCCCAGCCG CCTGGCTGTG 
ACCCAGCAGC TGAGTGATTC AGGTCAGAAG ACATTTAAAA GGAGAAGATC TATCATAAAC 
TGGGCCTTTT GGCGGGGTTC TAGCATTCAC CTGGATAACT TGCCTGTGTC ACCAACGTCT 
CCTATCCCAG GACAGCTCTT CGGGGTTTCT CTGCCAGATA TTTGTGAGAA TGACAATCTG 
CCCAAAGCTG TCTTGGATAT GCTTTTATTT CTTAATCAGA AAGGACCTCT CACAAAAGGC 
ATCTTCAGAC AGTCGGCCAA TGTGAAATCC TGCAGAGAGC TGAAAGAAAA ACTGAATTCC 
GGAGCTGAAG TACAACTAGA CTGTGAATCT ATTTTCGTAA TAGCATCTGT CTTAAAGGAT 
TTTCTACGAA ATATTCCAGG AAGTATTTTT TCATCAGATC TCTATGATCA CTGGGTCTGT 
GTAATGGATC AAGGAAATGA TGAAGAGAAA ATAAATGCAG TTCAAAGGCT GTTAGACCAG 
CTTCCGAGAG CCAATGTTCT TCTCCTAAGG TATCTTTTTG GGTTATTACA CAACATTGAG 
CAACATTCTT CATCCAATCA GATGACCGCA TTTAATTTAG CAGTGTGCAT CGCCCCAAGC 
ATTCTTTGGC CTCCTACTTC CTCTAGCCCA GAACTAGAAA ACGAATTTAC AAAAAAGGTT 
TCTCTGCTTA TACAGTTTCT TATTGAAAAC TGCTGTCGGA TATTTGGAGA AGAAATCACT 
TCCCTCTTGG GAGAGGTTTC AGTGAGATGT GATACCAGAG AGAATGCCTC AGATATCTCT 
TGCTTCCAGC TGAATGACTC CTCCTATGAC AGCTTGGAAA ATGAGCTGAA TGACGATGTT 
GACGCCCCAT GTCGTGACTT GGTAAAGAAA CTTGGTCAGG GTAGCAGAAG CATGGACTCC 
GTGTTAACCC TCAGTGACTA TGACCTTGAC CAGCCTGAGG TGGAAGGCCT TTTAACCCTG 
AGTGACTTTG ACTTAGGCCA TTCTAAAGAT GAAGACATTC AAATGGGACA GCCCCTTGAG 
TCCAAGCCAG TGGACACTGC AGTGTACACC AAGGTCCCAC GCGCCCAGGC CGCCTCTGGC 
GCGAGCACAC CCAGCCGCCT GTCCACAGCT GCCGCAGATG CTCCAAAAGG CCTGAGGCGA 
CACCGCCGCT GCTCGGAGCC CAGCATCGAC TATCTCGATT CAAAGCTTTC CTATCTCAGG 
GAGTTGTATC AGAAAAAGCT ACGCAAGTCC AGCTGTGATG CCATTCTCTC AAAAAAAGAT 
GAGAGCCATC TGAATCAGAA TCAACCCCTT CAGGAAGGGG GTAAGACATG TTTTAAAAAG 
AGTTTAGTCA CAGGCATTGA TAGCAAGAAA AATGCCGCTA GTAAAAATGT TAAGAAGAAA 
AGTTTGTCTG ATAATGAAGG AAGTCATGTA AAACTTTTTC CTAAATCCAA GCCAGTAGCC 
ATTTCCGTGG CATCGTACAG TCACATGTCC TCCTGTGATC ATCCCAGGAG CCAGCCCTTT 
GACATGGATG CATCCGAATA CTCCCCGCCA CACACTGCAG ACACCCTCAA GAGTTCAAGG 
AGGCAACGGC GCTGCTCAGA GCCCAGCATT GATGACCAGC ACTGCAGACT GACCTATCTC 
AGGGGGATTT ATCCAAAGAA GCAGCAGAAA GCCAGCTACG AAGCCAGTCT CTTGCATGGA 
GAGGAAGATT ATCTCAAGCG GCACAAGTCT TTACAAATGG AGGGCCAAAA GCTTATTAAT 
CAGAGTTTGG TCATGGGGAT TGAGGTGGGC AAGAGTAGTG CCACAAACCA AAACGTGGAG 
AAGGTTTTAC CCCCAAGTTT AAACCTTTGC TCAAGGACTA GCTATTCCAG CTTGTCCTCC 
CCAGGCACTT CCCCATCTGG TTCATCAGTA AGCTCCCAAG ATAGTGCTTT TTCTCAGATT 
TCTGAACACT CTGTGTTTAC ACCCACTGAA ACATCCTCTC CAATAGATTG TACTTTTCAA 
AGAAAACAGG CAGAGCTTTC TTCTGACTTT AGCAGTTCTG GCCTCATTTC TGGAATTCCT 
GGTCCCTCAT CAGGGCAGGC CAGCGGCCGC CTGGCCTATA CAAAAAAGGA CAGCGTAGAG 
TGGCATTCAC ACATGCATTC TGTAACTCTT CACCCCAGCA CATGGTTGAA GAGTGGTGTA 
GCCAGTTTGA AAAACTGGTC TCTCAAAAAG AAGGCAAGGG CACCCAGACC AGAGGAAAAT 
AAAGTGGGTT CTCTAAAAGG ACCCACGGAT CCTCCTGCAC ATGCTTCTGG TGTTCCGGAA 
GCCATCACAC TACAAGAAAG GCAGAAAGAC GTGCCCCAAA GGGCAGCTGA AGGACTGGGA 
GCTGTTCAGA CGGCCCGCTG GTATAGTTCC TACCCCAGCC AGGACTCAGA GAAATACTGT 
AGCTCTCTGT TCAGCCTCGT GGAAGACAGA CTTGGGCTTC ATATGAAGTC GCCCGAGGAA 
GGAAAGGATA GCGGGCAGTG CTCTCCTGGT ATGCTGCCCC AGAGAAGATC CTCCCCCGGC 
TCTTTGACTG TGGATGATGC GTCCAGCCCA GACTCAGGGC CGCCCGTGGT GTGTGATGCT 
GAGGATAGAC ATTTGGCCAA AGACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215840.1
CDS	181..3630
Translation	MKTLAERRRSAPSLILDKALQKRPSTKESPSASLDTCAFLSSFVCSSRTLLIDGRVELKRGLQRQERHLFLFNDLFVVAKIKYNNNFKIKNKIKLSDMWTASCVDEVGEGNTNATKSFVLGWPTVNFVATFSSPEQKDKWLSLLQRYINLEKEKDYPKSIPLKIFAKDIGNCAYSKTITVTNSDTANEVINMSLPMLGITGSEKEYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDTALLTQGSKDSPTPSNFQEPFLMEQLPREMQCQFILKPSRLAVTQQLSDSGQKTFKRRRSIINWAFWRGSSIHLDNLPVSPTSPIPGQLFGVSLPDICENDNLPKAVLDMLLFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGAEVQLDCESIFVIASVLKDFLRNIPGSIFSSDLYDHWVCVMDQGNDEEKINAVQRLLDQLPRANVLLLRYLFGLLHNIEQHSSSNQMTAFNLAVCIAPSILWPPTSSSPELENEFTKKVSLLIQFLIENCCRIFGEEITSLLGEVSVRCDTRENASDISCFQLNDSSYDSLENELNDDVDAPCRDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLGHSKDEDIQMGQPLESKPVDTAVYTKVPRAQAASGASTPSRLSTAAADAPKGLRRHRRCSEPSIDYLDSKLSYLRELYQKKLRKSSCDAILSKKDESHLNQNQPLQEGGKTCFKKSLVTGIDSKKNAASKNVKKKSLSDNEGSHVKLFPKSKPVAISVASYSHMSSCDHPRSQPFDMDASEYSPPHTADTLKSSRRQRRCSEPSIDDQHCRLTYLRGIYPKKQQKASYEASLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNVEKVLPPSLNLCSRTSYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQRKQAELSSDFSSSGLISGIPGPSSGQASGRLAYTKKDSVEWHSHMHSVTLHPSTWLKSGVASLKNWSLKKKARAPRPEENKVGSLKGPTDPPAHASGVPEAITLQERQKDVPQRAAEGLGAVQTARWYSSYPSQDSEKYCSSLFSLVEDRLGLHMKSPEEGKDSGQCSPGMLPQRRSSPGSLTVDDASSPDSGPPVVCDAEDRHLAKDM

Target ORF information:

RefSeq Version	XM_005215783.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 20 (ARHGAP20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215783.3

ORF Insert Sequence:

ATGAAAACAT TAGCAGAAAG GAGGAGGAGT GCTCCATCTC TTATCCTGGA TAAAGCCCTG 
CAAAAGCGGC CCAGTACCAA AGAAAGTCCT TCTGCTAGCC TGGACACATG TGCATTTCTG 
TCATCATTTG TGTGCTCCAG TAGGACTCTG CTTATTGATG GCCGTGTAGA ACTCAAGAGA 
GGCCTGCAGA GGCAGGAGCG ACATCTTTTC CTGTTCAATG ATCTGTTTGT TGTGGCCAAA 
ATCAAATATA ACAATAACTT TAAGATAAAA AATAAAATAA AATTAAGTGA CATGTGGACA 
GCAAGCTGTG TGGATGAAGT GGGAGAAGGC AACACCAATG CCACGAAATC CTTTGTCTTG 
GGCTGGCCCA CAGTGAACTT CGTGGCCACT TTCAGTTCTC CAGAACAGAA GGACAAATGG 
CTCTCTCTCC TTCAAAGATA CATCAATCTA GAGAAAGAAA AGGACTACCC GAAGAGCATT 
CCCCTCAAAA TCTTCGCCAA GGACATTGGG AATTGTGCCT ATTCTAAAAC TATAACAGTA 
ACGAATTCAG ATACAGCAAA TGAAGTTATC AACATGTCAC TACCAATGCT AGGGATAACT 
GGCTCTGAGA AAGAGTACCA GTTATGGGTC AATTCTGGCA AAGAAGAGGC ACCATACCCA 
CTTATTGGGC ATGAATATCC CTATGGAATT AAAATGAGCC ATCTTCGAGA CACTGCACTC 
CTGACACAGG GATCAAAGGA CTCCCCCACA CCTTCCAACT TCCAAGAGCC CTTCCTCATG 
GAGCAGCTGC CCCGAGAGAT GCAGTGTCAG TTCATCCTGA AGCCCAGCCG CCTGGCTGTG 
ACCCAGCAGC TGAGTGATTC AGGTCAGAAG ACATTTAAAA GGAGAAGATC TATCATAAAC 
TGGGCCTTTT GGCGGGGTTC TAGCATTCAC CTGGATAACT TGCCTGTGTC ACCAACGTCT 
CCTATCCCAG GACAGCTCTT CGGGGTTTCT CTGCCAGATA TTTGTGAGAA TGACAATCTG 
CCCAAAGCTG TCTTGGATAT GCTTTTATTT CTTAATCAGA AAGGACCTCT CACAAAAGGC 
ATCTTCAGAC AGTCGGCCAA TGTGAAATCC TGCAGAGAGC TGAAAGAAAA ACTGAATTCC 
GGAGCTGAAG TACAACTAGA CTGTGAATCT ATTTTCGTAA TAGCATCTGT CTTAAAGGAT 
TTTCTACGAA ATATTCCAGG AAGTATTTTT TCATCAGATC TCTATGATCA CTGGGTCTGT 
GTAATGGATC AAGGAAATGA TGAAGAGAAA ATAAATGCAG TTCAAAGGCT GTTAGACCAG 
CTTCCGAGAG CCAATGTTCT TCTCCTAAGG TATCTTTTTG GGTTATTACA CAACATTGAG 
CAACATTCTT CATCCAATCA GATGACCGCA TTTAATTTAG CAGTGTGCAT CGCCCCAAGC 
ATTCTTTGGC CTCCTACTTC CTCTAGCCCA GAACTAGAAA ACGAATTTAC AAAAAAGGTT 
TCTCTGCTTA TACAGTTTCT TATTGAAAAC TGCTGTCGGA TATTTGGAGA AGAAATCACT 
TCCCTCTTGG GAGAGGTTTC AGTGAGATGT GATACCAGAG AGAATGCCTC AGATATCTCT 
TGCTTCCAGC TGAATGACTC CTCCTATGAC AGCTTGGAAA ATGAGCTGAA TGACGATGTT 
GACGCCCCAT GTCGTGACTT GGTAAAGAAA CTTGGTCAGG GTAGCAGAAG CATGGACTCC 
GTGTTAACCC TCAGTGACTA TGACCTTGAC CAGCCTGAGG TGGAAGGCCT TTTAACCCTG 
AGTGACTTTG ACTTAGGCCA TTCTAAAGAT GAAGACATTC AAATGGGACA GCCCCTTGAG 
TCCAAGCCAG TGGACACTGC AGTGTACACC AAGGTCCCAC GCGCCCAGGC CGCCTCTGGC 
GCGAGCACAC CCAGCCGCCT GTCCACAGCT GCCGCAGATG CTCCAAAAGG CCTGAGGCGA 
CACCGCCGCT GCTCGGAGCC CAGCATCGAC TATCTCGATT CAAAGCTTTC CTATCTCAGG 
GAGTTGTATC AGAAAAAGCT ACGCAAGTCC AGCTGTGATG CCATTCTCTC AAAAAAAGAT 
GAGAGCCATC TGAATCAGAA TCAACCCCTT CAGGAAGGGG GTAAGACATG TTTTAAAAAG 
AGTTTAGTCA CAGGCATTGA TAGCAAGAAA AATGCCGCTA GTAAAAATGT TAAGAAGAAA 
AGTTTGTCTG ATAATGAAGG AAGTCATGTA AAACTTTTTC CTAAATCCAA GCCAGTAGCC 
ATTTCCGTGG CATCGTACAG TCACATGTCC TCCTGTGATC ATCCCAGGAG CCAGCCCTTT 
GACATGGATG CATCCGAATA CTCCCCGCCA CACACTGCAG ACACCCTCAA GAGTTCAAGG 
AGGCAACGGC GCTGCTCAGA GCCCAGCATT GATGACCAGC ACTGCAGACT GACCTATCTC 
AGGGGGATTT ATCCAAAGAA GCAGCAGAAA GCCAGCTACG AAGCCAGTCT CTTGCATGGA 
GAGGAAGATT ATCTCAAGCG GCACAAGTCT TTACAAATGG AGGGCCAAAA GCTTATTAAT 
CAGAGTTTGG TCATGGGGAT TGAGGTGGGC AAGAGTAGTG CCACAAACCA AAACGTGGAG 
AAGGTTTTAC CCCCAAGTTT AAACCTTTGC TCAAGGACTA GCTATTCCAG CTTGTCCTCC 
CCAGGCACTT CCCCATCTGG TTCATCAGTA AGCTCCCAAG ATAGTGCTTT TTCTCAGATT 
TCTGAACACT CTGTGTTTAC ACCCACTGAA ACATCCTCTC CAATAGATTG TACTTTTCAA 
AGAAAACAGG CAGAGCTTTC TTCTGACTTT AGCAGTTCTG GCCTCATTTC TGGAATTCCT 
GGTCCCTCAT CAGGGCAGGC CAGCGGCCGC CTGGCCTATA CAAAAAAGGA CAGCGTAGAG 
TGGCATTCAC ACATGCATTC TGTAACTCTT CACCCCAGCA CATGGTTGAA GAGTGGTGTA 
GCCAGTTTGA AAAACTGGTC TCTCAAAAAG AAGGCAAGGG CACCCAGACC AGAGGAAAAT 
AAAGTGGGTT CTCTAAAAGG ACCCACGGAT CCTCCTGCAC ATGCTTCTGG TGTTCCGGAA 
GCCATCACAC TACAAGAAAG GCAGAAAGAC GTGCCCCAAA GGGCAGCTGA AGGACTGGGA 
GCTGTTCAGA CGGCCCGCTG GTATAGTTCC TACCCCAGCC AGGACTCAGA GAAATACTGT 
AGCTCTCTGT TCAGCCTCGT GGAAGACAGA CTTGGGCTTC ATATGAAGTC GCCCGAGGAA 
GGAAAGGATA GCGGGCAGTG CTCTCCTGGT ATGCTGCCCC AGAGAAGATC CTCCCCCGGC 
TCTTTGACTG TGGATGATGC GTCCAGCCCA GACTCAGGGC CGCCCGTGGT GTGTGATGCT 
GAGGATAGAC ATTTGGCCAA AGACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP20 ( XM_024975249.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831017.1 Bos taurus Rho GTPase activating protein 20 (ARHGAP20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa26195
Clone ID Related Accession (Same CDS sequence)	XM_005215783.4 , XM_005215783.3 , XM_024975249.1
Accession Version	XM_024975249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACAT TAGCAGAAAG GAGGAGGAGT GCTCCATCTC TTATCCTGGA TAAAGCCCTG 
CAAAAGCGGC CCAGTACCAA AGAAAGTCCT TCTGCTAGCC TGGACACATG TGCATTTCTG 
TCATCATTTG TGTGCTCCAG TAGGACTCTG CTTATTGATG GCCGTGTAGA ACTCAAGAGA 
GGCCTGCAGA GGCAGGAGCG ACATCTTTTC CTGTTCAATG ATCTGTTTGT TGTGGCCAAA 
ATCAAATATA ACAATAACTT TAAGATAAAA AATAAAATAA AATTAAGTGA CATGTGGACA 
GCAAGCTGTG TGGATGAAGT GGGAGAAGGC AACACCAATG CCACGAAATC CTTTGTCTTG 
GGCTGGCCCA CAGTGAACTT CGTGGCCACT TTCAGTTCTC CAGAACAGAA GGACAAATGG 
CTCTCTCTCC TTCAAAGATA CATCAATCTA GAGAAAGAAA AGGACTACCC GAAGAGCATT 
CCCCTCAAAA TCTTCGCCAA GGACATTGGG AATTGTGCCT ATTCTAAAAC TATAACAGTA 
ACGAATTCAG ATACAGCAAA TGAAGTTATC AACATGTCAC TACCAATGCT AGGGATAACT 
GGCTCTGAGA AAGAGTACCA GTTATGGGTC AATTCTGGCA AAGAAGAGGC ACCATACCCA 
CTTATTGGGC ATGAATATCC CTATGGAATT AAAATGAGCC ATCTTCGAGA CACTGCACTC 
CTGACACAGG GATCAAAGGA CTCCCCCACA CCTTCCAACT TCCAAGAGCC CTTCCTCATG 
GAGCAGCTGC CCCGAGAGAT GCAGTGTCAG TTCATCCTGA AGCCCAGCCG CCTGGCTGTG 
ACCCAGCAGC TGAGTGATTC AGGTCAGAAG ACATTTAAAA GGAGAAGATC TATCATAAAC 
TGGGCCTTTT GGCGGGGTTC TAGCATTCAC CTGGATAACT TGCCTGTGTC ACCAACGTCT 
CCTATCCCAG GACAGCTCTT CGGGGTTTCT CTGCCAGATA TTTGTGAGAA TGACAATCTG 
CCCAAAGCTG TCTTGGATAT GCTTTTATTT CTTAATCAGA AAGGACCTCT CACAAAAGGC 
ATCTTCAGAC AGTCGGCCAA TGTGAAATCC TGCAGAGAGC TGAAAGAAAA ACTGAATTCC 
GGAGCTGAAG TACAACTAGA CTGTGAATCT ATTTTCGTAA TAGCATCTGT CTTAAAGGAT 
TTTCTACGAA ATATTCCAGG AAGTATTTTT TCATCAGATC TCTATGATCA CTGGGTCTGT 
GTAATGGATC AAGGAAATGA TGAAGAGAAA ATAAATGCAG TTCAAAGGCT GTTAGACCAG 
CTTCCGAGAG CCAATGTTCT TCTCCTAAGG TATCTTTTTG GGTTATTACA CAACATTGAG 
CAACATTCTT CATCCAATCA GATGACCGCA TTTAATTTAG CAGTGTGCAT CGCCCCAAGC 
ATTCTTTGGC CTCCTACTTC CTCTAGCCCA GAACTAGAAA ACGAATTTAC AAAAAAGGTT 
TCTCTGCTTA TACAGTTTCT TATTGAAAAC TGCTGTCGGA TATTTGGAGA AGAAATCACT 
TCCCTCTTGG GAGAGGTTTC AGTGAGATGT GATACCAGAG AGAATGCCTC AGATATCTCT 
TGCTTCCAGC TGAATGACTC CTCCTATGAC AGCTTGGAAA ATGAGCTGAA TGACGATGTT 
GACGCCCCAT GTCGTGACTT GGTAAAGAAA CTTGGTCAGG GTAGCAGAAG CATGGACTCC 
GTGTTAACCC TCAGTGACTA TGACCTTGAC CAGCCTGAGG TGGAAGGCCT TTTAACCCTG 
AGTGACTTTG ACTTAGGCCA TTCTAAAGAT GAAGACATTC AAATGGGACA GCCCCTTGAG 
TCCAAGCCAG TGGACACTGC AGTGTACACC AAGGTCCCAC GCGCCCAGGC CGCCTCTGGC 
GCGAGCACAC CCAGCCGCCT GTCCACAGCT GCCGCAGATG CTCCAAAAGG CCTGAGGCGA 
CACCGCCGCT GCTCGGAGCC CAGCATCGAC TATCTCGATT CAAAGCTTTC CTATCTCAGG 
GAGTTGTATC AGAAAAAGCT ACGCAAGTCC AGCTGTGATG CCATTCTCTC AAAAAAAGAT 
GAGAGCCATC TGAATCAGAA TCAACCCCTT CAGGAAGGGG GTAAGACATG TTTTAAAAAG 
AGTTTAGTCA CAGGCATTGA TAGCAAGAAA AATGCCGCTA GTAAAAATGT TAAGAAGAAA 
AGTTTGTCTG ATAATGAAGG AAGTCATGTA AAACTTTTTC CTAAATCCAA GCCAGTAGCC 
ATTTCCGTGG CATCGTACAG TCACATGTCC TCCTGTGATC ATCCCAGGAG CCAGCCCTTT 
GACATGGATG CATCCGAATA CTCCCCGCCA CACACTGCAG ACACCCTCAA GAGTTCAAGG 
AGGCAACGGC GCTGCTCAGA GCCCAGCATT GATGACCAGC ACTGCAGACT GACCTATCTC 
AGGGGGATTT ATCCAAAGAA GCAGCAGAAA GCCAGCTACG AAGCCAGTCT CTTGCATGGA 
GAGGAAGATT ATCTCAAGCG GCACAAGTCT TTACAAATGG AGGGCCAAAA GCTTATTAAT 
CAGAGTTTGG TCATGGGGAT TGAGGTGGGC AAGAGTAGTG CCACAAACCA AAACGTGGAG 
AAGGTTTTAC CCCCAAGTTT AAACCTTTGC TCAAGGACTA GCTATTCCAG CTTGTCCTCC 
CCAGGCACTT CCCCATCTGG TTCATCAGTA AGCTCCCAAG ATAGTGCTTT TTCTCAGATT 
TCTGAACACT CTGTGTTTAC ACCCACTGAA ACATCCTCTC CAATAGATTG TACTTTTCAA 
AGAAAACAGG CAGAGCTTTC TTCTGACTTT AGCAGTTCTG GCCTCATTTC TGGAATTCCT 
GGTCCCTCAT CAGGGCAGGC CAGCGGCCGC CTGGCCTATA CAAAAAAGGA CAGCGTAGAG 
TGGCATTCAC ACATGCATTC TGTAACTCTT CACCCCAGCA CATGGTTGAA GAGTGGTGTA 
GCCAGTTTGA AAAACTGGTC TCTCAAAAAG AAGGCAAGGG CACCCAGACC AGAGGAAAAT 
AAAGTGGGTT CTCTAAAAGG ACCCACGGAT CCTCCTGCAC ATGCTTCTGG TGTTCCGGAA 
GCCATCACAC TACAAGAAAG GCAGAAAGAC GTGCCCCAAA GGGCAGCTGA AGGACTGGGA 
GCTGTTCAGA CGGCCCGCTG GTATAGTTCC TACCCCAGCC AGGACTCAGA GAAATACTGT 
AGCTCTCTGT TCAGCCTCGT GGAAGACAGA CTTGGGCTTC ATATGAAGTC GCCCGAGGAA 
GGAAAGGATA GCGGGCAGTG CTCTCCTGGT ATGCTGCCCC AGAGAAGATC CTCCCCCGGC 
TCTTTGACTG TGGATGATGC GTCCAGCCCA GACTCAGGGC CGCCCGTGGT GTGTGATGCT 
GAGGATAGAC ATTTGGCCAA AGACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831017.1
CDS	465..3914
Translation	MKTLAERRRSAPSLILDKALQKRPSTKESPSASLDTCAFLSSFVCSSRTLLIDGRVELKRGLQRQERHLFLFNDLFVVAKIKYNNNFKIKNKIKLSDMWTASCVDEVGEGNTNATKSFVLGWPTVNFVATFSSPEQKDKWLSLLQRYINLEKEKDYPKSIPLKIFAKDIGNCAYSKTITVTNSDTANEVINMSLPMLGITGSEKEYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDTALLTQGSKDSPTPSNFQEPFLMEQLPREMQCQFILKPSRLAVTQQLSDSGQKTFKRRRSIINWAFWRGSSIHLDNLPVSPTSPIPGQLFGVSLPDICENDNLPKAVLDMLLFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGAEVQLDCESIFVIASVLKDFLRNIPGSIFSSDLYDHWVCVMDQGNDEEKINAVQRLLDQLPRANVLLLRYLFGLLHNIEQHSSSNQMTAFNLAVCIAPSILWPPTSSSPELENEFTKKVSLLIQFLIENCCRIFGEEITSLLGEVSVRCDTRENASDISCFQLNDSSYDSLENELNDDVDAPCRDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLGHSKDEDIQMGQPLESKPVDTAVYTKVPRAQAASGASTPSRLSTAAADAPKGLRRHRRCSEPSIDYLDSKLSYLRELYQKKLRKSSCDAILSKKDESHLNQNQPLQEGGKTCFKKSLVTGIDSKKNAASKNVKKKSLSDNEGSHVKLFPKSKPVAISVASYSHMSSCDHPRSQPFDMDASEYSPPHTADTLKSSRRQRRCSEPSIDDQHCRLTYLRGIYPKKQQKASYEASLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNVEKVLPPSLNLCSRTSYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQRKQAELSSDFSSSGLISGIPGPSSGQASGRLAYTKKDSVEWHSHMHSVTLHPSTWLKSGVASLKNWSLKKKARAPRPEENKVGSLKGPTDPPAHASGVPEAITLQERQKDVPQRAAEGLGAVQTARWYSSYPSQDSEKYCSSLFSLVEDRLGLHMKSPEEGKDSGQCSPGMLPQRRSSPGSLTVDDASSPDSGPPVVCDAEDRHLAKDM

Target ORF information:

RefSeq Version	XM_024975249.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 20 (ARHGAP20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975249.1

ORF Insert Sequence:

ATGAAAACAT TAGCAGAAAG GAGGAGGAGT GCTCCATCTC TTATCCTGGA TAAAGCCCTG 
CAAAAGCGGC CCAGTACCAA AGAAAGTCCT TCTGCTAGCC TGGACACATG TGCATTTCTG 
TCATCATTTG TGTGCTCCAG TAGGACTCTG CTTATTGATG GCCGTGTAGA ACTCAAGAGA 
GGCCTGCAGA GGCAGGAGCG ACATCTTTTC CTGTTCAATG ATCTGTTTGT TGTGGCCAAA 
ATCAAATATA ACAATAACTT TAAGATAAAA AATAAAATAA AATTAAGTGA CATGTGGACA 
GCAAGCTGTG TGGATGAAGT GGGAGAAGGC AACACCAATG CCACGAAATC CTTTGTCTTG 
GGCTGGCCCA CAGTGAACTT CGTGGCCACT TTCAGTTCTC CAGAACAGAA GGACAAATGG 
CTCTCTCTCC TTCAAAGATA CATCAATCTA GAGAAAGAAA AGGACTACCC GAAGAGCATT 
CCCCTCAAAA TCTTCGCCAA GGACATTGGG AATTGTGCCT ATTCTAAAAC TATAACAGTA 
ACGAATTCAG ATACAGCAAA TGAAGTTATC AACATGTCAC TACCAATGCT AGGGATAACT 
GGCTCTGAGA AAGAGTACCA GTTATGGGTC AATTCTGGCA AAGAAGAGGC ACCATACCCA 
CTTATTGGGC ATGAATATCC CTATGGAATT AAAATGAGCC ATCTTCGAGA CACTGCACTC 
CTGACACAGG GATCAAAGGA CTCCCCCACA CCTTCCAACT TCCAAGAGCC CTTCCTCATG 
GAGCAGCTGC CCCGAGAGAT GCAGTGTCAG TTCATCCTGA AGCCCAGCCG CCTGGCTGTG 
ACCCAGCAGC TGAGTGATTC AGGTCAGAAG ACATTTAAAA GGAGAAGATC TATCATAAAC 
TGGGCCTTTT GGCGGGGTTC TAGCATTCAC CTGGATAACT TGCCTGTGTC ACCAACGTCT 
CCTATCCCAG GACAGCTCTT CGGGGTTTCT CTGCCAGATA TTTGTGAGAA TGACAATCTG 
CCCAAAGCTG TCTTGGATAT GCTTTTATTT CTTAATCAGA AAGGACCTCT CACAAAAGGC 
ATCTTCAGAC AGTCGGCCAA TGTGAAATCC TGCAGAGAGC TGAAAGAAAA ACTGAATTCC 
GGAGCTGAAG TACAACTAGA CTGTGAATCT ATTTTCGTAA TAGCATCTGT CTTAAAGGAT 
TTTCTACGAA ATATTCCAGG AAGTATTTTT TCATCAGATC TCTATGATCA CTGGGTCTGT 
GTAATGGATC AAGGAAATGA TGAAGAGAAA ATAAATGCAG TTCAAAGGCT GTTAGACCAG 
CTTCCGAGAG CCAATGTTCT TCTCCTAAGG TATCTTTTTG GGTTATTACA CAACATTGAG 
CAACATTCTT CATCCAATCA GATGACCGCA TTTAATTTAG CAGTGTGCAT CGCCCCAAGC 
ATTCTTTGGC CTCCTACTTC CTCTAGCCCA GAACTAGAAA ACGAATTTAC AAAAAAGGTT 
TCTCTGCTTA TACAGTTTCT TATTGAAAAC TGCTGTCGGA TATTTGGAGA AGAAATCACT 
TCCCTCTTGG GAGAGGTTTC AGTGAGATGT GATACCAGAG AGAATGCCTC AGATATCTCT 
TGCTTCCAGC TGAATGACTC CTCCTATGAC AGCTTGGAAA ATGAGCTGAA TGACGATGTT 
GACGCCCCAT GTCGTGACTT GGTAAAGAAA CTTGGTCAGG GTAGCAGAAG CATGGACTCC 
GTGTTAACCC TCAGTGACTA TGACCTTGAC CAGCCTGAGG TGGAAGGCCT TTTAACCCTG 
AGTGACTTTG ACTTAGGCCA TTCTAAAGAT GAAGACATTC AAATGGGACA GCCCCTTGAG 
TCCAAGCCAG TGGACACTGC AGTGTACACC AAGGTCCCAC GCGCCCAGGC CGCCTCTGGC 
GCGAGCACAC CCAGCCGCCT GTCCACAGCT GCCGCAGATG CTCCAAAAGG CCTGAGGCGA 
CACCGCCGCT GCTCGGAGCC CAGCATCGAC TATCTCGATT CAAAGCTTTC CTATCTCAGG 
GAGTTGTATC AGAAAAAGCT ACGCAAGTCC AGCTGTGATG CCATTCTCTC AAAAAAAGAT 
GAGAGCCATC TGAATCAGAA TCAACCCCTT CAGGAAGGGG GTAAGACATG TTTTAAAAAG 
AGTTTAGTCA CAGGCATTGA TAGCAAGAAA AATGCCGCTA GTAAAAATGT TAAGAAGAAA 
AGTTTGTCTG ATAATGAAGG AAGTCATGTA AAACTTTTTC CTAAATCCAA GCCAGTAGCC 
ATTTCCGTGG CATCGTACAG TCACATGTCC TCCTGTGATC ATCCCAGGAG CCAGCCCTTT 
GACATGGATG CATCCGAATA CTCCCCGCCA CACACTGCAG ACACCCTCAA GAGTTCAAGG 
AGGCAACGGC GCTGCTCAGA GCCCAGCATT GATGACCAGC ACTGCAGACT GACCTATCTC 
AGGGGGATTT ATCCAAAGAA GCAGCAGAAA GCCAGCTACG AAGCCAGTCT CTTGCATGGA 
GAGGAAGATT ATCTCAAGCG GCACAAGTCT TTACAAATGG AGGGCCAAAA GCTTATTAAT 
CAGAGTTTGG TCATGGGGAT TGAGGTGGGC AAGAGTAGTG CCACAAACCA AAACGTGGAG 
AAGGTTTTAC CCCCAAGTTT AAACCTTTGC TCAAGGACTA GCTATTCCAG CTTGTCCTCC 
CCAGGCACTT CCCCATCTGG TTCATCAGTA AGCTCCCAAG ATAGTGCTTT TTCTCAGATT 
TCTGAACACT CTGTGTTTAC ACCCACTGAA ACATCCTCTC CAATAGATTG TACTTTTCAA 
AGAAAACAGG CAGAGCTTTC TTCTGACTTT AGCAGTTCTG GCCTCATTTC TGGAATTCCT 
GGTCCCTCAT CAGGGCAGGC CAGCGGCCGC CTGGCCTATA CAAAAAAGGA CAGCGTAGAG 
TGGCATTCAC ACATGCATTC TGTAACTCTT CACCCCAGCA CATGGTTGAA GAGTGGTGTA 
GCCAGTTTGA AAAACTGGTC TCTCAAAAAG AAGGCAAGGG CACCCAGACC AGAGGAAAAT 
AAAGTGGGTT CTCTAAAAGG ACCCACGGAT CCTCCTGCAC ATGCTTCTGG TGTTCCGGAA 
GCCATCACAC TACAAGAAAG GCAGAAAGAC GTGCCCCAAA GGGCAGCTGA AGGACTGGGA 
GCTGTTCAGA CGGCCCGCTG GTATAGTTCC TACCCCAGCC AGGACTCAGA GAAATACTGT 
AGCTCTCTGT TCAGCCTCGT GGAAGACAGA CTTGGGCTTC ATATGAAGTC GCCCGAGGAA 
GGAAAGGATA GCGGGCAGTG CTCTCCTGGT ATGCTGCCCC AGAGAAGATC CTCCCCCGGC 
TCTTTGACTG TGGATGATGC GTCCAGCCCA GACTCAGGGC CGCCCGTGGT GTGTGATGCT 
GAGGATAGAC ATTTGGCCAA AGACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ARHGAP20 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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