USE1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	USE1
Entrez Gene ID	55850
Full Name	unconventional SNARE in the ER 1
Synonyms	D12,MDS032,P31,SLT1
General protein information	Preferred Names unconventional SNARE in the ER 1 Names vesicle transport protein USE1 Q-SNARE SNARE-like tail-anchored protein 1 homolog USE1-like protein protein p31 putative MAPK activating protein PM26 unconventional SNARE in the ER 1 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.11
Disorder MIM:	610675

mRNA and Protein(s)

mRNA	Protein	Name
XM_017026975.1	XP_016882464.1	vesicle transport protein USE1 isoform X1
NM_018467.4	NP_060937.2	vesicle transport protein USE1
XM_017026976.1	XP_016882465.1	vesicle transport protein USE1 isoform X1
NM_018467.3	NP_060937.1	vesicle transport protein USE1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	USE1	XP_003432947.2
Danio rerio (zebrafish)	use1	NP_956618.1
Bos taurus (cattle)	USE1	NP_001069052.1
Mus musculus (house mouse)	Use1	NP_001139252.1
Drosophila melanogaster (fruit fly)	Use1	NP_648289.2
Xenopus tropicalis (tropical clawed frog)	use1	NP_001120491.1
Homo sapiens (human)	USE1	NP_060937.1
	USE1	XP_001113229.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005893	XP_315922.3
Pan troglodytes (chimpanzee)	USE1	XP_512478.1
Rattus norvegicus (Norway rat)	Use1	NP_001162571.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

LMO2 blocks the UBA6-USE1 interaction and downstream FAT10ylation by targeting the ubiquitin fold domain of UBA6.
Wu C, Liu Y, Gu X, Zhu T, Yang S, Sun W
Biochemical and biophysical research communications478(3)1442-8(2016 09)

Investigations into the auto-FAT10ylation of the bispecific E2 conjugating enzyme UBA6-specific E2 enzyme 1.
Aichem A, Catone N, Groettrup M
The FEBS journal281(7)1848-59(2014 Apr)

Grsf1-induced translation of the SNARE protein Use1 is required for expansion of the erythroid compartment.
Nieradka A, Ufer C, Thiadens K, Grech G, Horos R, van Coevorden-Hameete M, van den Akker E, Sofi S, Kuhn H, von Lindern M
PloS one9(9)e104631(2014)

USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis.
Aichem A, Pelzer C, Lukasiak S, Kalveram B, Sheppard PW, Rani N, Schmidtke G, Groettrup M
Nature communications113(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study revealed a novel function of LMO2 involving in the regulatory hierarchy of UBA6-USE1-FAT10ylation pathway by targeting the E1 enzyme UBA6.
Title: LMO2 blocks the UBA6-USE1 interaction and downstream FAT10ylation by targeting the ubiquitin fold domain of UBA6.

FAT10 ubiquitination of USE1 leads to its accelerated degradation by the proteasome.
Title: Investigations into the auto-FAT10ylation of the bispecific E2 conjugating enzyme UBA6-specific E2 enzyme 1.

Grsf1 controls translation of the protein Use1, possibly by positioning the 40S ribosomal subunit and associated translation factors in front of the translation start site.
Title: Grsf1-induced translation of the SNARE protein Use1 is required for expansion of the erythroid compartment.

USE1 is not only the first E2 enzyme but also the first known substrate of FAT10 conjugation, as it was efficiently auto-FAT10ylated in cis but not in trans.
Title: USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis.

Results together suggest that NAG links between p31 and ZW10-RINT-1 and is involved in Golgi-to-ER transport.
Title: Identification of the neuroblastoma-amplified gene product as a component of the syntaxin 18 complex implicated in Golgi-to-endoplasmic reticulum retrograde transport.

The following USE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31134	NM_018467.3 Latest version!	Homo sapiens unconventional SNARE in the ER 1 (USE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu88557	NM_018467.4 Latest version!	Homo sapiens unconventional SNARE in the ER 1 (USE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu88557	XM_017026975.1 Latest version!	Homo sapiens unconventional SNARE in the ER 1 (USE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu88558	XM_017026976.1 Latest version!	Homo sapiens unconventional SNARE in the ER 1 (USE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USE1 ( NM_018467.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060937.1 Homo sapiens unconventional SNARE in the ER 1 (USE1), mRNA.

Price & Availability↑

CloneID	OHu31134
Clone ID Related Accession (Same CDS sequence)	NM_018467.3
Accession Version	NM_018467.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	vesicle transport protein USE1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA506853.1 and BC008455.1. On Aug 1, 2007 this sequence version replaced NM_018467.2. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.596951.1, SRR1163658.374106.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGT CGAGGCTGGA GCTAAACCTG GTGCGGCTGC TATCCCGCTG CGAGGCGATG 
GCAGCGGAGA AACGGGACCC GGACGAGTGG CGCCTGGAGA AGTACGTGGG AGCCCTAGAG 
GACATGTTGC AGGCCCTGAA GGTCCACGCG AGCAAACCGG CCTCTGAGGT GATCAATGAA 
TATTCCTGGA AGGTGGATTT TCTGAAGGGG ATGCTGCAAG CCGAGAAGCT GACCTCCTCC 
TCAGAGAAAG CACTGGCCAA CCAGTTCCTG GCCCCTGGCC GTGTGCCAAC CACAGCCAGA 
GAGCGAGTGC CCGCCACAAA GACGGTGCAT CTGCAGTCAC GGGCGCGGTA CACCAGCGAG 
ATGCGGAGTG AGCTACTAGG CACGGACTCT GCAGAGCCTG AGATGGACGT AAGGAAGAGA 
ACTGGAGTGG CAGGGTCCCA GCCAGTGAGT GAGAAGCAGT CGGCAGCTGA GCTAGACCTC 
GTCCTGCAGC GACATCAGAA CCTCCAGGAA AAGCTGGCGG AAGAGATGCT AGGACTGGCC 
CGGAGCCTCA AGACCAATAC CCTGGCCGCC CAGAGTGTCA TCAAGAAGGA CAACCAGACC 
CTGTCACACT CACTGAAAAT GGCGGACCAG AACCTGGAGA AACTGAAGAC GGAGTCAGAG 
CGTCTGGAGC AGCACACGCA GAAGTCAGTC AACTGGCTGC TCTGGGCCAT GCTCATTATC 
GTCTGCTTCA TCTTCATTAG CATGATCCTC TTCATTCGAA TCATGCCTAA ACTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060937.1
CDS	61..840
Translation	MAASRLELNLVRLLSRCEAMAAEKRDPDEWRLEKYVGALEDMLQALKVHASKPASEVINEYSWKVDFLKGMLQAEKLTSSSEKALANQFLAPGRVPTTARERVPATKTVHLQSRARYTSEMRSELLGTDSAEPEMDVRKRTGVAGSQPVSEKQSAAELDLVLQRHQNLQEKLAEEMLGLARSLKTNTLAAQSVIKKDNQTLSHSLKMADQNLEKLKTESERLEQHTQKSVNWLLWAMLIIVCFIFISMILFIRIMPKLK

Target ORF information:

RefSeq Version	NM_018467.3
Organism	Homo sapiens(human)
Definition	Homo sapiens unconventional SNARE in the ER 1 (USE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018467.3

ORF Insert Sequence:

ATGGCGGCGT CGAGGCTGGA GCTAAACCTG GTGCGGCTGC TATCCCGCTG CGAGGCGATG 
GCAGCGGAGA AACGGGACCC GGACGAGTGG CGCCTGGAGA AGTACGTGGG AGCCCTAGAG 
GACATGTTGC AGGCCCTGAA GGTCCACGCG AGCAAACCGG CCTCTGAGGT GATCAATGAA 
TATTCCTGGA AGGTGGATTT TCTGAAGGGG ATGCTGCAAG CCGAGAAGCT GACCTCCTCC 
TCAGAGAAAG CACTGGCCAA CCAGTTCCTG GCCCCTGGCC GTGTGCCAAC CACAGCCAGA 
GAGCGAGTGC CCGCCACAAA GACGGTGCAT CTGCAGTCAC GGGCGCGGTA CACCAGCGAG 
ATGCGGAGTG AGCTACTAGG CACGGACTCT GCAGAGCCTG AGATGGACGT AAGGAAGAGA 
ACTGGAGTGG CAGGGTCCCA GCCAGTGAGT GAGAAGCAGT CGGCAGCTGA GCTAGACCTC 
GTCCTGCAGC GACATCAGAA CCTCCAGGAA AAGCTGGCGG AAGAGATGCT AGGACTGGCC 
CGGAGCCTCA AGACCAATAC CCTGGCCGCC CAGAGTGTCA TCAAGAAGGA CAACCAGACC 
CTGTCACACT CACTGAAAAT GGCGGACCAG AACCTGGAGA AACTGAAGAC GGAGTCAGAG 
CGTCTGGAGC AGCACACGCA GAAGTCAGTC AACTGGCTGC TCTGGGCCAT GCTCATTATC 
GTCTGCTTCA TCTTCATTAG CATGATCCTC TTCATTCGAA TCATGCCTAA ACTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USE1 ( NM_018467.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060937.2 Homo sapiens unconventional SNARE in the ER 1 (USE1), mRNA.

Price & Availability↑

CloneID	OHu88557
Clone ID Related Accession (Same CDS sequence)	XM_017026975.1 , NM_018467.4
Accession Version	NM_018467.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	vesicle transport protein USE1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC020913.9. On Nov 23, 2018 this sequence version replaced NM_018467.3. ##Evidence-Data-START## Transcript exon combination :: SRR5189652.251445.1, SRR5189667.84173.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263897.10/ ENSP00000263897.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGT CGAGGCTGGA GCTAAACCTG GTGCGGCTGC TATCCCGCTG CGAGGCGATG 
GCAGCGGAGA AACGGGACCC GGACGAGTGG CGCCTGGAGA AGTACGTGGG AGCCCTAGAG 
GACATGTTGC AGGCCCTGAA GGTCCACGCG AGCAAACCGG CCTCTGAGGT GATCAATGAA 
TATTCCTGGA AGGTGGATTT TCTGAAGGGG ATGCTGCAAG CCGAGAAGCT GACCTCCTCC 
TCAGAGAAAG CACTGGCCAA CCAGTTCCTG GCCCCTGGCC GTGTGCCAAC CACAGCCAGA 
GAGCGAGTGC CCGCCACAAA GACGGTGCAT CTGCAGTCAC GGGCGCGGTA CACCAGCGAG 
ATGCGGAGTG AGCTACTAGG CACGGACTCT GCAGAGCCTG AGATGGACGT AAGGAAGAGA 
ACTGGAGTGG CAGGGTCCCA GCCAGTGAGT GAGAAGCAGT TGGCAGCTGA GCTAGACCTC 
GTCCTGCAGC GACATCAGAA CCTCCAGGAA AAGCTGGCGG AAGAGATGCT AGGACTGGCC 
CGGAGCCTCA AGACCAATAC CCTGGCCGCC CAGAGTGTCA TCAAGAAGGA CAACCAGACC 
CTGTCACACT CACTGAAAAT GGCGGACCAG AACCTGGAGA AACTGAAGAC GGAGTCAGAG 
CGTCTGGAGC AGCACACGCA GAAGTCAGTC AACTGGCTGC TCTGGGCCAT GCTCATTATC 
GTCTGCTTCA TCTTCATTAG CATGATCCTC TTCATTCGAA TCATGCCTAA ACTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060937.2
CDS	50..829
Translation	MAASRLELNLVRLLSRCEAMAAEKRDPDEWRLEKYVGALEDMLQALKVHASKPASEVINEYSWKVDFLKGMLQAEKLTSSSEKALANQFLAPGRVPTTARERVPATKTVHLQSRARYTSEMRSELLGTDSAEPEMDVRKRTGVAGSQPVSEKQLAAELDLVLQRHQNLQEKLAEEMLGLARSLKTNTLAAQSVIKKDNQTLSHSLKMADQNLEKLKTESERLEQHTQKSVNWLLWAMLIIVCFIFISMILFIRIMPKLK

Target ORF information:

RefSeq Version	NM_018467.4
Organism	Homo sapiens(human)
Definition	Homo sapiens unconventional SNARE in the ER 1 (USE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018467.4

ORF Insert Sequence:

ATGGCGGCGT CGAGGCTGGA GCTAAACCTG GTGCGGCTGC TATCCCGCTG CGAGGCGATG 
GCAGCGGAGA AACGGGACCC GGACGAGTGG CGCCTGGAGA AGTACGTGGG AGCCCTAGAG 
GACATGTTGC AGGCCCTGAA GGTCCACGCG AGCAAACCGG CCTCTGAGGT GATCAATGAA 
TATTCCTGGA AGGTGGATTT TCTGAAGGGG ATGCTGCAAG CCGAGAAGCT GACCTCCTCC 
TCAGAGAAAG CACTGGCCAA CCAGTTCCTG GCCCCTGGCC GTGTGCCAAC CACAGCCAGA 
GAGCGAGTGC CCGCCACAAA GACGGTGCAT CTGCAGTCAC GGGCGCGGTA CACCAGCGAG 
ATGCGGAGTG AGCTACTAGG CACGGACTCT GCAGAGCCTG AGATGGACGT AAGGAAGAGA 
ACTGGAGTGG CAGGGTCCCA GCCAGTGAGT GAGAAGCAGT TGGCAGCTGA GCTAGACCTC 
GTCCTGCAGC GACATCAGAA CCTCCAGGAA AAGCTGGCGG AAGAGATGCT AGGACTGGCC 
CGGAGCCTCA AGACCAATAC CCTGGCCGCC CAGAGTGTCA TCAAGAAGGA CAACCAGACC 
CTGTCACACT CACTGAAAAT GGCGGACCAG AACCTGGAGA AACTGAAGAC GGAGTCAGAG 
CGTCTGGAGC AGCACACGCA GAAGTCAGTC AACTGGCTGC TCTGGGCCAT GCTCATTATC 
GTCTGCTTCA TCTTCATTAG CATGATCCTC TTCATTCGAA TCATGCCTAA ACTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USE1 ( XM_017026975.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882464.1 Homo sapiens unconventional SNARE in the ER 1 (USE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu88557
Clone ID Related Accession (Same CDS sequence)	XM_017026975.1 , NM_018467.4
Accession Version	XM_017026975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	vesicle transport protein USE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGT CGAGGCTGGA GCTAAACCTG GTGCGGCTGC TATCCCGCTG CGAGGCGATG 
GCAGCGGAGA AACGGGACCC GGACGAGTGG CGCCTGGAGA AGTACGTGGG AGCCCTAGAG 
GACATGTTGC AGGCCCTGAA GGTCCACGCG AGCAAACCGG CCTCTGAGGT GATCAATGAA 
TATTCCTGGA AGGTGGATTT TCTGAAGGGG ATGCTGCAAG CCGAGAAGCT GACCTCCTCC 
TCAGAGAAAG CACTGGCCAA CCAGTTCCTG GCCCCTGGCC GTGTGCCAAC CACAGCCAGA 
GAGCGAGTGC CCGCCACAAA GACGGTGCAT CTGCAGTCAC GGGCGCGGTA CACCAGCGAG 
ATGCGGAGTG AGCTACTAGG CACGGACTCT GCAGAGCCTG AGATGGACGT AAGGAAGAGA 
ACTGGAGTGG CAGGGTCCCA GCCAGTGAGT GAGAAGCAGT TGGCAGCTGA GCTAGACCTC 
GTCCTGCAGC GACATCAGAA CCTCCAGGAA AAGCTGGCGG AAGAGATGCT AGGACTGGCC 
CGGAGCCTCA AGACCAATAC CCTGGCCGCC CAGAGTGTCA TCAAGAAGGA CAACCAGACC 
CTGTCACACT CACTGAAAAT GGCGGACCAG AACCTGGAGA AACTGAAGAC GGAGTCAGAG 
CGTCTGGAGC AGCACACGCA GAAGTCAGTC AACTGGCTGC TCTGGGCCAT GCTCATTATC 
GTCTGCTTCA TCTTCATTAG CATGATCCTC TTCATTCGAA TCATGCCTAA ACTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882464.1
CDS	771..1550
Translation	MAASRLELNLVRLLSRCEAMAAEKRDPDEWRLEKYVGALEDMLQALKVHASKPASEVINEYSWKVDFLKGMLQAEKLTSSSEKALANQFLAPGRVPTTARERVPATKTVHLQSRARYTSEMRSELLGTDSAEPEMDVRKRTGVAGSQPVSEKQLAAELDLVLQRHQNLQEKLAEEMLGLARSLKTNTLAAQSVIKKDNQTLSHSLKMADQNLEKLKTESERLEQHTQKSVNWLLWAMLIIVCFIFISMILFIRIMPKLK

Target ORF information:

RefSeq Version	XM_017026975.1
Organism	Homo sapiens(human)
Definition	Homo sapiens unconventional SNARE in the ER 1 (USE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026975.1

ORF Insert Sequence:

ATGGCGGCGT CGAGGCTGGA GCTAAACCTG GTGCGGCTGC TATCCCGCTG CGAGGCGATG 
GCAGCGGAGA AACGGGACCC GGACGAGTGG CGCCTGGAGA AGTACGTGGG AGCCCTAGAG 
GACATGTTGC AGGCCCTGAA GGTCCACGCG AGCAAACCGG CCTCTGAGGT GATCAATGAA 
TATTCCTGGA AGGTGGATTT TCTGAAGGGG ATGCTGCAAG CCGAGAAGCT GACCTCCTCC 
TCAGAGAAAG CACTGGCCAA CCAGTTCCTG GCCCCTGGCC GTGTGCCAAC CACAGCCAGA 
GAGCGAGTGC CCGCCACAAA GACGGTGCAT CTGCAGTCAC GGGCGCGGTA CACCAGCGAG 
ATGCGGAGTG AGCTACTAGG CACGGACTCT GCAGAGCCTG AGATGGACGT AAGGAAGAGA 
ACTGGAGTGG CAGGGTCCCA GCCAGTGAGT GAGAAGCAGT TGGCAGCTGA GCTAGACCTC 
GTCCTGCAGC GACATCAGAA CCTCCAGGAA AAGCTGGCGG AAGAGATGCT AGGACTGGCC 
CGGAGCCTCA AGACCAATAC CCTGGCCGCC CAGAGTGTCA TCAAGAAGGA CAACCAGACC 
CTGTCACACT CACTGAAAAT GGCGGACCAG AACCTGGAGA AACTGAAGAC GGAGTCAGAG 
CGTCTGGAGC AGCACACGCA GAAGTCAGTC AACTGGCTGC TCTGGGCCAT GCTCATTATC 
GTCTGCTTCA TCTTCATTAG CATGATCCTC TTCATTCGAA TCATGCCTAA ACTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USE1 ( XM_017026976.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882465.1 Homo sapiens unconventional SNARE in the ER 1 (USE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu88558
Clone ID Related Accession (Same CDS sequence)	XM_017026976.1
Accession Version	XM_017026976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	vesicle transport protein USE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAAG CCGAGAAGCT GACCTCCTCC TCAGAGAAAG CACTGGCCAA CCAGTTCCTG 
GCCCCTGGCC GTGTGCCAAC CACAGCCAGA GAGCGAGTGC CCGCCACAAA GACGGTGCAT 
CTGCAGTCAC GGGCGCGGTA CACCAGCGAG ATGCGGAGTG AGCTACTAGG CACGGACTCT 
GCAGAGCCTG AGATGGACGT AAGGAAGAGA ACTGGAGTGG CAGGGTCCCA GCCAGTGAGT 
GAGAAGCAGT TGGCAGCTGA GCTAGACCTC GTCCTGCAGC GACATCAGAA CCTCCAGGAA 
AAGCTGGCGG AAGAGATGCT AGGACTGGCC CGGAGCCTCA AGACCAATAC CCTGGCCGCC 
CAGAGTGTCA TCAAGAAGGA CAACCAGACC CTGTCACACT CACTGAAAAT GGCGGACCAG 
AACCTGGAGA AACTGAAGAC GGAGTCAGAG CGTCTGGAGC AGCACACGCA GAAGTCAGTC 
AACTGGCTGC TCTGGGCCAT GCTCATTATC GTCTGCTTCA TCTTCATTAG CATGATCCTC 
TTCATTCGAA TCATGCCTAA ACTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882465.1
CDS	221..790
Translation	MLQAEKLTSSSEKALANQFLAPGRVPTTARERVPATKTVHLQSRARYTSEMRSELLGTDSAEPEMDVRKRTGVAGSQPVSEKQLAAELDLVLQRHQNLQEKLAEEMLGLARSLKTNTLAAQSVIKKDNQTLSHSLKMADQNLEKLKTESERLEQHTQKSVNWLLWAMLIIVCFIFISMILFIRIMPKLK

Target ORF information:

RefSeq Version	XM_017026976.1
Organism	Homo sapiens(human)
Definition	Homo sapiens unconventional SNARE in the ER 1 (USE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026976.1

ORF Insert Sequence:

ATGCTGCAAG CCGAGAAGCT GACCTCCTCC TCAGAGAAAG CACTGGCCAA CCAGTTCCTG 
GCCCCTGGCC GTGTGCCAAC CACAGCCAGA GAGCGAGTGC CCGCCACAAA GACGGTGCAT 
CTGCAGTCAC GGGCGCGGTA CACCAGCGAG ATGCGGAGTG AGCTACTAGG CACGGACTCT 
GCAGAGCCTG AGATGGACGT AAGGAAGAGA ACTGGAGTGG CAGGGTCCCA GCCAGTGAGT 
GAGAAGCAGT TGGCAGCTGA GCTAGACCTC GTCCTGCAGC GACATCAGAA CCTCCAGGAA 
AAGCTGGCGG AAGAGATGCT AGGACTGGCC CGGAGCCTCA AGACCAATAC CCTGGCCGCC 
CAGAGTGTCA TCAAGAAGGA CAACCAGACC CTGTCACACT CACTGAAAAT GGCGGACCAG 
AACCTGGAGA AACTGAAGAC GGAGTCAGAG CGTCTGGAGC AGCACACGCA GAAGTCAGTC 
AACTGGCTGC TCTGGGCCAT GCTCATTATC GTCTGCTTCA TCTTCATTAG CATGATCCTC 
TTCATTCGAA TCATGCCTAA ACTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

LMO2 blocks the UBA6-USE1 interaction and downstream FAT10ylation by targeting the ubiquitin fold domain of UBA6.
Biochemical and biophysical research communications478(3)1442-8(2016 09)
Wu C,Liu Y,Gu X,Zhu T,Yang S,Sun W

Investigations into the auto-FAT10ylation of the bispecific E2 conjugating enzyme UBA6-specific E2 enzyme 1.
The FEBS journal281(7)1848-59(2014 Apr)
Aichem A,Catone N,Groettrup M

Grsf1-induced translation of the SNARE protein Use1 is required for expansion of the erythroid compartment.
PloS one9(9)e104631(2014)
Nieradka A,Ufer C,Thiadens K,Grech G,Horos R,van Coevorden-Hameete M,van den Akker E,Sofi S,Kuhn H,von Lindern M

USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis.
Nature communications113(2010 May)
Aichem A,Pelzer C,Lukasiak S,Kalveram B,Sheppard PW,Rani N,Schmidtke G,Groettrup M

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USE1 cDNA ORF clone, Homo sapiens(human)

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