PKM cDNA ORF clone, Homo sapiens(human)

The following PKM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PKM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu20031 NM_182471.4
Latest version!
Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20031 NM_182470.3
Latest version!
Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20143 NM_001206796.3
Latest version!
Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu20299 NM_002654.6
Latest version!
Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20031 XM_017022313.2
Latest version!
Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20299 XM_005254445.5
Latest version!
Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu19800 NM_001206797.2
Latest version!
Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu20044 NM_001206798.2
Latest version!
Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu20230 NM_001206799.2
Latest version!
Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu59743 NM_001316318.2
Latest version!
Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu48374 XM_006720570.1
Latest version!
Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu48375 XM_005254443.1
Latest version!
Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu59742 XM_011521670.2
Latest version!
Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
View Old Accession Versions >>
OHu20031 NM_182471.2 Homo sapiens pyruvate kinase, muscle (PKM), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20299 XM_005254445.3 Homo sapiens pyruvate kinase, muscle (PKM), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu59743 NM_001316318.1 Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu20031 NM_182470.2 Homo sapiens pyruvate kinase, muscle (PKM), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20143 NM_001206796.2 Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu20299 NM_002654.4 Homo sapiens pyruvate kinase, muscle (PKM), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20031 NM_182471.3 Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20230 NM_001206799.1 Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu20299 XM_005254445.4 Homo sapiens pyruvate kinase, muscle (PKM), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20031 XM_017022313.1 Homo sapiens pyruvate kinase, muscle (PKM), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20299 NM_002654.5 Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu20031
    Clone ID Related Accession (Same CDS sequence) NM_182471.2 , NM_182471.3 , NM_182470.3 , NM_182470.2 , XM_017022313.1 , NM_182471.4 , XM_017022313.2
    Accession Version NM_182471.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product pyruvate kinase PKM isoform b
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATAGCTCGTG AGGCTGAGGC AGCCATGTTC CACCGCAAGC TGTTTGAAGA ACTTGTGCGA
    GCCTCAAGTC ACTCCACAGA CCTCATGGAA GCCATGGCCA TGGGCAGCGT GGAGGCTTCT
    TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTGACGGAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_872271.1
    Translation

    Target ORF information:

    RefSeq Version NM_182471.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens pyruvate kinase, muscle (PKM), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_182471.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATAGCTCGTG AGGCTGAGGC AGCCATGTTC CACCGCAAGC TGTTTGAAGA ACTTGTGCGA
    GCCTCAAGTC ACTCCACAGA CCTCATGGAA GCCATGGCCA TGGGCAGCGT GGAGGCTTCT
    TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTGACGGAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20031
    Clone ID Related Accession (Same CDS sequence) NM_182471.2 , NM_182471.3 , NM_182470.3 , NM_182470.2 , XM_017022313.1 , NM_182471.4 , XM_017022313.2
    Accession Version NM_182471.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC326232.1, AC020779.10, DC376054.1, BC023592.2 and BC035198.1. On Oct 14, 2015 this sequence version replaced NM_182471.2. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (b, also called M1) is shorter at the N-terminus compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.32715.1, SRR1803612.133436.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATAGCTCGTG AGGCTGAGGC AGCCATGTTC CACCGCAAGC TGTTTGAAGA ACTTGTGCGA
    GCCTCAAGTC ACTCCACAGA CCTCATGGAA GCCATGGCCA TGGGCAGCGT GGAGGCTTCT
    TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTGACGGAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_872271.1
    CDS523..2118
    Translation

    Target ORF information:

    RefSeq Version NM_182471.3
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_182471.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATAGCTCGTG AGGCTGAGGC AGCCATGTTC CACCGCAAGC TGTTTGAAGA ACTTGTGCGA
    GCCTCAAGTC ACTCCACAGA CCTCATGGAA GCCATGGCCA TGGGCAGCGT GGAGGCTTCT
    TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTGACGGAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20031
    Clone ID Related Accession (Same CDS sequence) NM_182471.2 , NM_182471.3 , NM_182470.3 , NM_182470.2 , XM_017022313.1 , NM_182471.4 , XM_017022313.2
    Accession Version NM_182470.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC326232.1, AC020779.10, DC376054.1, AK092369.1, BC023592.2 and BC035198.1. On Oct 14, 2015 this sequence version replaced NM_182470.2. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (b, also called M1) is shorter at the N-terminus compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK092369.1, SRR1660805.242291.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATAGCTCGTG AGGCTGAGGC AGCCATGTTC CACCGCAAGC TGTTTGAAGA ACTTGTGCGA
    GCCTCAAGTC ACTCCACAGA CCTCATGGAA GCCATGGCCA TGGGCAGCGT GGAGGCTTCT
    TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTGACGGAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_872270.1
    CDS739..2334
    Translation

    Target ORF information:

    RefSeq Version NM_182470.3
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_182470.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATAGCTCGTG AGGCTGAGGC AGCCATGTTC CACCGCAAGC TGTTTGAAGA ACTTGTGCGA
    GCCTCAAGTC ACTCCACAGA CCTCATGGAA GCCATGGCCA TGGGCAGCGT GGAGGCTTCT
    TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTGACGGAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20031
    Clone ID Related Accession (Same CDS sequence) NM_182471.2 , NM_182471.3 , NM_182470.3 , NM_182470.2 , XM_017022313.1 , NM_182471.4 , XM_017022313.2
    Accession Version NM_182470.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product pyruvate kinase PKM isoform b
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATAGCTCGTG AGGCTGAGGC AGCCATGTTC CACCGCAAGC TGTTTGAAGA ACTTGTGCGA
    GCCTCAAGTC ACTCCACAGA CCTCATGGAA GCCATGGCCA TGGGCAGCGT GGAGGCTTCT
    TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTGACGGAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_872270.1
    Translation

    Target ORF information:

    RefSeq Version NM_182470.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens pyruvate kinase, muscle (PKM), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_182470.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATAGCTCGTG AGGCTGAGGC AGCCATGTTC CACCGCAAGC TGTTTGAAGA ACTTGTGCGA
    GCCTCAAGTC ACTCCACAGA CCTCATGGAA GCCATGGCCA TGGGCAGCGT GGAGGCTTCT
    TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTGACGGAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20031
    Clone ID Related Accession (Same CDS sequence) NM_182471.2 , NM_182471.3 , NM_182470.3 , NM_182470.2 , XM_017022313.1 , NM_182471.4 , XM_017022313.2
    Accession Version NM_182471.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020779.10. On May 31, 2019 this sequence version replaced NM_182471.3. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (b, also called M1) is shorter at the N-terminus compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.985026.1, SRR3476690.840092.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATAGCTCGTG AGGCTGAGGC AGCCATGTTC CACCGCAAGC TGTTTGAAGA ACTTGTGCGA
    GCCTCAAGTC ACTCCACAGA CCTCATGGAA GCCATGGCCA TGGGCAGCGT GGAGGCTTCT
    TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTGACGGAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_872271.1
    CDS89..1684
    Translation

    Target ORF information:

    RefSeq Version NM_182471.4
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_182471.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATAGCTCGTG AGGCTGAGGC AGCCATGTTC CACCGCAAGC TGTTTGAAGA ACTTGTGCGA
    GCCTCAAGTC ACTCCACAGA CCTCATGGAA GCCATGGCCA TGGGCAGCGT GGAGGCTTCT
    TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTGACGGAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20143
    Clone ID Related Accession (Same CDS sequence) NM_001206796.3 , NM_001206796.2
    Accession Version NM_001206796.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020779.10. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001206796.2. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (c). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.569268.1, AK294315.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCAGTGGA GCTCAGAGAG AGGAGAACGG CTCCTCACGC CTGGGGCCTG CTCTTCAGAA 
    GTCCCCAGCG CCGTTCCTTC CAGATCAGGC GGCTCTCCAG GGCACACCGT ATTCAGCTCT
    GAGCGGTCTT TGCTAGTGAG GCCAAGGAGC CACCCTGAGC CAAAAGGGGA GCATTATGTC
    ACCGGAAGCC CAACCCCAGA GAACCAAAGG ACCTCAGCAG CCATGTCGAA GCCCCATAGT
    GAAGCCGGGA CTGCCTTCAT TCAGACCCAG CAGCTGCACG CAGCCATGGC TGACACATTC
    CTGGAGCACA TGTGCCGCCT GGACATTGAT TCACCACCCA TCACAGCCCG GAACACTGGC
    ATCATCTGTA CCATTGGCCC AGCTTCCCGA TCAGTGGAGA CGTTGAAGGA GATGATTAAG
    TCTGGAATGA ATGTGGCTCG TCTGAACTTC TCTCATGGAA CTCATGAGTA CCATGCGGAG
    ACCATCAAGA ATGTGCGCAC AGCCACGGAA AGCTTTGCTT CTGACCCCAT CCTCTACCGG
    CCCGTTGCTG TGGCTCTAGA CACTAAAGGA CCTGAGATCC GAACTGGGCT CATCAAGGGC
    AGCGGCACTG CAGAGGTGGA GCTGAAGAAG GGAGCCACTC TCAAAATCAC GCTGGATAAC
    GCCTACATGG AAAAGTGTGA CGAGAACATC CTGTGGCTGG ACTACAAGAA CATCTGCAAG
    GTGGTGGAAG TGGGCAGCAA GATCTACGTG GATGATGGGC TTATTTCTCT CCAGGTGAAG
    CAGAAAGGTG CCGACTTCCT GGTGACGGAG GTGGAAAATG GTGGCTCCTT GGGCAGCAAG
    AAGGGTGTGA ACCTTCCTGG GGCTGCTGTG GACTTGCCTG CTGTGTCGGA GAAGGACATC
    CAGGATCTGA AGTTTGGGGT CGAGCAGGAT GTTGATATGG TGTTTGCGTC ATTCATCCGC
    AAGGCATCTG ATGTCCATGA AGTTAGGAAG GTCCTGGGAG AGAAGGGAAA GAACATCAAG
    ATTATCAGCA AAATCGAGAA TCATGAGGGG GTTCGGAGGT TTGATGAAAT CCTGGAGGCC
    AGTGATGGGA TCATGGTGGC TCGTGGTGAT CTAGGCATTG AGATTCCTGC AGAGAAGGTC
    TTCCTTGCTC AGAAGATGAT GATTGGACGG TGCAACCGAG CTGGGAAGCC TGTCATCTGT
    GCTACTCAGA TGCTGGAGAG CATGATCAAG AAGCCCCGCC CCACTCGGGC TGAAGGCAGT
    GATGTGGCCA ATGCAGTCCT GGATGGAGCC GACTGCATCA TGCTGTCTGG AGAAACAGCC
    AAAGGGGACT ATCCTCTGGA GGCTGTGCGC ATGCAGCACC TGATAGCTCG TGAGGCTGAG
    GCAGCCATGT TCCACCGCAA GCTGTTTGAA GAACTTGTGC GAGCCTCAAG TCACTCCACA
    GACCTCATGG AAGCCATGGC CATGGGCAGC GTGGAGGCTT CTTATAAGTG TTTAGCAGCA
    GCTTTGATAG TTCTGACGGA GTCTGGCAGG TCTGCTCACC AGGTGGCCAG ATACCGCCCA
    CGTGCCCCCA TCATTGCTGT GACCCGGAAT CCCCAGACAG CTCGTCAGGC CCACCTGTAC
    CGTGGCATCT TCCCTGTGCT GTGCAAGGAC CCAGTCCAGG AGGCCTGGGC TGAGGACGTG
    GACCTCCGGG TGAACTTTGC CATGAATGTT GGCAAGGCCC GAGGCTTCTT CAAGAAGGGA
    GATGTGGTCA TTGTGCTGAC CGGATGGCGC CCTGGCTCCG GCTTCACCAA CACCATGCGT
    GTTGTTCCTG TGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193725.1
    CDS204..2021
    Translation

    Target ORF information:

    RefSeq Version NM_001206796.3
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206796.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCAGTGGA GCTCAGAGAG AGGAGAACGG CTCCTCACGC CTGGGGCCTG CTCTTCAGAA 
    GTCCCCAGCG CCGTTCCTTC CAGATCAGGC GGCTCTCCAG GGCACACCGT ATTCAGCTCT
    GAGCGGTCTT TGCTAGTGAG GCCAAGGAGC CACCCTGAGC CAAAAGGGGA GCATTATGTC
    ACCGGAAGCC CAACCCCAGA GAACCAAAGG ACCTCAGCAG CCATGTCGAA GCCCCATAGT
    GAAGCCGGGA CTGCCTTCAT TCAGACCCAG CAGCTGCACG CAGCCATGGC TGACACATTC
    CTGGAGCACA TGTGCCGCCT GGACATTGAT TCACCACCCA TCACAGCCCG GAACACTGGC
    ATCATCTGTA CCATTGGCCC AGCTTCCCGA TCAGTGGAGA CGTTGAAGGA GATGATTAAG
    TCTGGAATGA ATGTGGCTCG TCTGAACTTC TCTCATGGAA CTCATGAGTA CCATGCGGAG
    ACCATCAAGA ATGTGCGCAC AGCCACGGAA AGCTTTGCTT CTGACCCCAT CCTCTACCGG
    CCCGTTGCTG TGGCTCTAGA CACTAAAGGA CCTGAGATCC GAACTGGGCT CATCAAGGGC
    AGCGGCACTG CAGAGGTGGA GCTGAAGAAG GGAGCCACTC TCAAAATCAC GCTGGATAAC
    GCCTACATGG AAAAGTGTGA CGAGAACATC CTGTGGCTGG ACTACAAGAA CATCTGCAAG
    GTGGTGGAAG TGGGCAGCAA GATCTACGTG GATGATGGGC TTATTTCTCT CCAGGTGAAG
    CAGAAAGGTG CCGACTTCCT GGTGACGGAG GTGGAAAATG GTGGCTCCTT GGGCAGCAAG
    AAGGGTGTGA ACCTTCCTGG GGCTGCTGTG GACTTGCCTG CTGTGTCGGA GAAGGACATC
    CAGGATCTGA AGTTTGGGGT CGAGCAGGAT GTTGATATGG TGTTTGCGTC ATTCATCCGC
    AAGGCATCTG ATGTCCATGA AGTTAGGAAG GTCCTGGGAG AGAAGGGAAA GAACATCAAG
    ATTATCAGCA AAATCGAGAA TCATGAGGGG GTTCGGAGGT TTGATGAAAT CCTGGAGGCC
    AGTGATGGGA TCATGGTGGC TCGTGGTGAT CTAGGCATTG AGATTCCTGC AGAGAAGGTC
    TTCCTTGCTC AGAAGATGAT GATTGGACGG TGCAACCGAG CTGGGAAGCC TGTCATCTGT
    GCTACTCAGA TGCTGGAGAG CATGATCAAG AAGCCCCGCC CCACTCGGGC TGAAGGCAGT
    GATGTGGCCA ATGCAGTCCT GGATGGAGCC GACTGCATCA TGCTGTCTGG AGAAACAGCC
    AAAGGGGACT ATCCTCTGGA GGCTGTGCGC ATGCAGCACC TGATAGCTCG TGAGGCTGAG
    GCAGCCATGT TCCACCGCAA GCTGTTTGAA GAACTTGTGC GAGCCTCAAG TCACTCCACA
    GACCTCATGG AAGCCATGGC CATGGGCAGC GTGGAGGCTT CTTATAAGTG TTTAGCAGCA
    GCTTTGATAG TTCTGACGGA GTCTGGCAGG TCTGCTCACC AGGTGGCCAG ATACCGCCCA
    CGTGCCCCCA TCATTGCTGT GACCCGGAAT CCCCAGACAG CTCGTCAGGC CCACCTGTAC
    CGTGGCATCT TCCCTGTGCT GTGCAAGGAC CCAGTCCAGG AGGCCTGGGC TGAGGACGTG
    GACCTCCGGG TGAACTTTGC CATGAATGTT GGCAAGGCCC GAGGCTTCTT CAAGAAGGGA
    GATGTGGTCA TTGTGCTGAC CGGATGGCGC CCTGGCTCCG GCTTCACCAA CACCATGCGT
    GTTGTTCCTG TGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20143
    Clone ID Related Accession (Same CDS sequence) NM_001206796.3 , NM_001206796.2
    Accession Version NM_001206796.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC326232.1, AC020779.10, DC376054.1, DA382029.1, AK294315.1, BC000481.2 and BC035198.1. This sequence is a reference standard in the RefSeqGene project. On Oct 14, 2015 this sequence version replaced NM_001206796.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (c). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294315.1, SRR1660805.187254.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCAGTGGA GCTCAGAGAG AGGAGAACGG CTCCTCACGC CTGGGGCCTG CTCTTCAGAA 
    GTCCCCAGCG CCGTTCCTTC CAGATCAGGC GGCTCTCCAG GGCACACCGT ATTCAGCTCT
    GAGCGGTCTT TGCTAGTGAG GCCAAGGAGC CACCCTGAGC CAAAAGGGGA GCATTATGTC
    ACCGGAAGCC CAACCCCAGA GAACCAAAGG ACCTCAGCAG CCATGTCGAA GCCCCATAGT
    GAAGCCGGGA CTGCCTTCAT TCAGACCCAG CAGCTGCACG CAGCCATGGC TGACACATTC
    CTGGAGCACA TGTGCCGCCT GGACATTGAT TCACCACCCA TCACAGCCCG GAACACTGGC
    ATCATCTGTA CCATTGGCCC AGCTTCCCGA TCAGTGGAGA CGTTGAAGGA GATGATTAAG
    TCTGGAATGA ATGTGGCTCG TCTGAACTTC TCTCATGGAA CTCATGAGTA CCATGCGGAG
    ACCATCAAGA ATGTGCGCAC AGCCACGGAA AGCTTTGCTT CTGACCCCAT CCTCTACCGG
    CCCGTTGCTG TGGCTCTAGA CACTAAAGGA CCTGAGATCC GAACTGGGCT CATCAAGGGC
    AGCGGCACTG CAGAGGTGGA GCTGAAGAAG GGAGCCACTC TCAAAATCAC GCTGGATAAC
    GCCTACATGG AAAAGTGTGA CGAGAACATC CTGTGGCTGG ACTACAAGAA CATCTGCAAG
    GTGGTGGAAG TGGGCAGCAA GATCTACGTG GATGATGGGC TTATTTCTCT CCAGGTGAAG
    CAGAAAGGTG CCGACTTCCT GGTGACGGAG GTGGAAAATG GTGGCTCCTT GGGCAGCAAG
    AAGGGTGTGA ACCTTCCTGG GGCTGCTGTG GACTTGCCTG CTGTGTCGGA GAAGGACATC
    CAGGATCTGA AGTTTGGGGT CGAGCAGGAT GTTGATATGG TGTTTGCGTC ATTCATCCGC
    AAGGCATCTG ATGTCCATGA AGTTAGGAAG GTCCTGGGAG AGAAGGGAAA GAACATCAAG
    ATTATCAGCA AAATCGAGAA TCATGAGGGG GTTCGGAGGT TTGATGAAAT CCTGGAGGCC
    AGTGATGGGA TCATGGTGGC TCGTGGTGAT CTAGGCATTG AGATTCCTGC AGAGAAGGTC
    TTCCTTGCTC AGAAGATGAT GATTGGACGG TGCAACCGAG CTGGGAAGCC TGTCATCTGT
    GCTACTCAGA TGCTGGAGAG CATGATCAAG AAGCCCCGCC CCACTCGGGC TGAAGGCAGT
    GATGTGGCCA ATGCAGTCCT GGATGGAGCC GACTGCATCA TGCTGTCTGG AGAAACAGCC
    AAAGGGGACT ATCCTCTGGA GGCTGTGCGC ATGCAGCACC TGATAGCTCG TGAGGCTGAG
    GCAGCCATGT TCCACCGCAA GCTGTTTGAA GAACTTGTGC GAGCCTCAAG TCACTCCACA
    GACCTCATGG AAGCCATGGC CATGGGCAGC GTGGAGGCTT CTTATAAGTG TTTAGCAGCA
    GCTTTGATAG TTCTGACGGA GTCTGGCAGG TCTGCTCACC AGGTGGCCAG ATACCGCCCA
    CGTGCCCCCA TCATTGCTGT GACCCGGAAT CCCCAGACAG CTCGTCAGGC CCACCTGTAC
    CGTGGCATCT TCCCTGTGCT GTGCAAGGAC CCAGTCCAGG AGGCCTGGGC TGAGGACGTG
    GACCTCCGGG TGAACTTTGC CATGAATGTT GGCAAGGCCC GAGGCTTCTT CAAGAAGGGA
    GATGTGGTCA TTGTGCTGAC CGGATGGCGC CCTGGCTCCG GCTTCACCAA CACCATGCGT
    GTTGTTCCTG TGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193725.1
    CDS638..2455
    Translation

    Target ORF information:

    RefSeq Version NM_001206796.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206796.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCAGTGGA GCTCAGAGAG AGGAGAACGG CTCCTCACGC CTGGGGCCTG CTCTTCAGAA 
    GTCCCCAGCG CCGTTCCTTC CAGATCAGGC GGCTCTCCAG GGCACACCGT ATTCAGCTCT
    GAGCGGTCTT TGCTAGTGAG GCCAAGGAGC CACCCTGAGC CAAAAGGGGA GCATTATGTC
    ACCGGAAGCC CAACCCCAGA GAACCAAAGG ACCTCAGCAG CCATGTCGAA GCCCCATAGT
    GAAGCCGGGA CTGCCTTCAT TCAGACCCAG CAGCTGCACG CAGCCATGGC TGACACATTC
    CTGGAGCACA TGTGCCGCCT GGACATTGAT TCACCACCCA TCACAGCCCG GAACACTGGC
    ATCATCTGTA CCATTGGCCC AGCTTCCCGA TCAGTGGAGA CGTTGAAGGA GATGATTAAG
    TCTGGAATGA ATGTGGCTCG TCTGAACTTC TCTCATGGAA CTCATGAGTA CCATGCGGAG
    ACCATCAAGA ATGTGCGCAC AGCCACGGAA AGCTTTGCTT CTGACCCCAT CCTCTACCGG
    CCCGTTGCTG TGGCTCTAGA CACTAAAGGA CCTGAGATCC GAACTGGGCT CATCAAGGGC
    AGCGGCACTG CAGAGGTGGA GCTGAAGAAG GGAGCCACTC TCAAAATCAC GCTGGATAAC
    GCCTACATGG AAAAGTGTGA CGAGAACATC CTGTGGCTGG ACTACAAGAA CATCTGCAAG
    GTGGTGGAAG TGGGCAGCAA GATCTACGTG GATGATGGGC TTATTTCTCT CCAGGTGAAG
    CAGAAAGGTG CCGACTTCCT GGTGACGGAG GTGGAAAATG GTGGCTCCTT GGGCAGCAAG
    AAGGGTGTGA ACCTTCCTGG GGCTGCTGTG GACTTGCCTG CTGTGTCGGA GAAGGACATC
    CAGGATCTGA AGTTTGGGGT CGAGCAGGAT GTTGATATGG TGTTTGCGTC ATTCATCCGC
    AAGGCATCTG ATGTCCATGA AGTTAGGAAG GTCCTGGGAG AGAAGGGAAA GAACATCAAG
    ATTATCAGCA AAATCGAGAA TCATGAGGGG GTTCGGAGGT TTGATGAAAT CCTGGAGGCC
    AGTGATGGGA TCATGGTGGC TCGTGGTGAT CTAGGCATTG AGATTCCTGC AGAGAAGGTC
    TTCCTTGCTC AGAAGATGAT GATTGGACGG TGCAACCGAG CTGGGAAGCC TGTCATCTGT
    GCTACTCAGA TGCTGGAGAG CATGATCAAG AAGCCCCGCC CCACTCGGGC TGAAGGCAGT
    GATGTGGCCA ATGCAGTCCT GGATGGAGCC GACTGCATCA TGCTGTCTGG AGAAACAGCC
    AAAGGGGACT ATCCTCTGGA GGCTGTGCGC ATGCAGCACC TGATAGCTCG TGAGGCTGAG
    GCAGCCATGT TCCACCGCAA GCTGTTTGAA GAACTTGTGC GAGCCTCAAG TCACTCCACA
    GACCTCATGG AAGCCATGGC CATGGGCAGC GTGGAGGCTT CTTATAAGTG TTTAGCAGCA
    GCTTTGATAG TTCTGACGGA GTCTGGCAGG TCTGCTCACC AGGTGGCCAG ATACCGCCCA
    CGTGCCCCCA TCATTGCTGT GACCCGGAAT CCCCAGACAG CTCGTCAGGC CCACCTGTAC
    CGTGGCATCT TCCCTGTGCT GTGCAAGGAC CCAGTCCAGG AGGCCTGGGC TGAGGACGTG
    GACCTCCGGG TGAACTTTGC CATGAATGTT GGCAAGGCCC GAGGCTTCTT CAAGAAGGGA
    GATGTGGTCA TTGTGCTGAC CGGATGGCGC CCTGGCTCCG GCTTCACCAA CACCATGCGT
    GTTGTTCCTG TGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20299
    Clone ID Related Accession (Same CDS sequence) XM_005254445.5 , XM_005254445.3 , NM_002654.5 , NM_002654.4 , XM_005254445.4 , NM_002654.6
    Accession Version NM_002654.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-17
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC326232.1, AC020779.10, DC376054.1, BC000481.2 and BC035198.1. On Oct 14, 2015 this sequence version replaced NM_002654.4. Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence and has an alternate in-frame coding exon compared to variant 4. The resulting isoform (a, also called M2) is shorter at the N-terminus and has a different internal segment compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794631.1, BC000481.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATTGCCCGTG AGGCAGAGGC TGCCATCTAC CACTTGCAAT TATTTGAGGA ACTCCGCCGC
    CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCCACCGCCG TGGGTGCCGT GGAGGCCTCC
    TTCAAGTGCT GCAGTGGGGC CATAATCGTC CTCACCAAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002645.3
    CDS523..2118
    Translation

    Target ORF information:

    RefSeq Version NM_002654.5
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002654.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATTGCCCGTG AGGCAGAGGC TGCCATCTAC CACTTGCAAT TATTTGAGGA ACTCCGCCGC
    CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCCACCGCCG TGGGTGCCGT GGAGGCCTCC
    TTCAAGTGCT GCAGTGGGGC CATAATCGTC CTCACCAAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20299
    Clone ID Related Accession (Same CDS sequence) XM_005254445.5 , XM_005254445.3 , NM_002654.5 , NM_002654.4 , XM_005254445.4 , NM_002654.6
    Accession Version NM_002654.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product pyruvate kinase PKM isoform a
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATTGCCCGTG AGGCAGAGGC TGCCATCTAC CACTTGCAAT TATTTGAGGA ACTCCGCCGC
    CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCCACCGCCG TGGGTGCCGT GGAGGCCTCC
    TTCAAGTGCT GCAGTGGGGC CATAATCGTC CTCACCAAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002645.3
    Translation

    Target ORF information:

    RefSeq Version NM_002654.4
    Organism Homo sapiens(Human)
    Definition Homo sapiens pyruvate kinase, muscle (PKM), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002654.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATTGCCCGTG AGGCAGAGGC TGCCATCTAC CACTTGCAAT TATTTGAGGA ACTCCGCCGC
    CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCCACCGCCG TGGGTGCCGT GGAGGCCTCC
    TTCAAGTGCT GCAGTGGGGC CATAATCGTC CTCACCAAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20299
    Clone ID Related Accession (Same CDS sequence) XM_005254445.5 , XM_005254445.3 , NM_002654.5 , NM_002654.4 , XM_005254445.4 , NM_002654.6
    Accession Version NM_002654.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020779.10. On Nov 23, 2018 this sequence version replaced NM_002654.5. Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence and has an alternate in-frame coding exon compared to variant 4. The resulting isoform (a, also called M2) is shorter at the N-terminus and has a different internal segment compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794631.1, SRR3476690.254571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology MANE Ensembl match :: ENST00000335181.10/ ENSP00000334983.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATTGCCCGTG AGGCAGAGGC TGCCATCTAC CACTTGCAAT TATTTGAGGA ACTCCGCCGC
    CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCCACCGCCG TGGGTGCCGT GGAGGCCTCC
    TTCAAGTGCT GCAGTGGGGC CATAATCGTC CTCACCAAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002645.3
    CDS89..1684
    Translation

    Target ORF information:

    RefSeq Version NM_002654.6
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002654.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATTGCCCGTG AGGCAGAGGC TGCCATCTAC CACTTGCAAT TATTTGAGGA ACTCCGCCGC
    CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCCACCGCCG TGGGTGCCGT GGAGGCCTCC
    TTCAAGTGCT GCAGTGGGGC CATAATCGTC CTCACCAAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20031
    Clone ID Related Accession (Same CDS sequence) NM_182471.2 , NM_182471.3 , NM_182470.3 , NM_182470.2 , XM_017022313.1 , NM_182471.4 , XM_017022313.2
    Accession Version XM_017022313.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATAGCTCGTG AGGCTGAGGC AGCCATGTTC CACCGCAAGC TGTTTGAAGA ACTTGTGCGA
    GCCTCAAGTC ACTCCACAGA CCTCATGGAA GCCATGGCCA TGGGCAGCGT GGAGGCTTCT
    TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTGACGGAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877802.1
    CDS84..1679
    Translation

    Target ORF information:

    RefSeq Version XM_017022313.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase, muscle (PKM), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATAGCTCGTG AGGCTGAGGC AGCCATGTTC CACCGCAAGC TGTTTGAAGA ACTTGTGCGA
    GCCTCAAGTC ACTCCACAGA CCTCATGGAA GCCATGGCCA TGGGCAGCGT GGAGGCTTCT
    TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTGACGGAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20031
    Clone ID Related Accession (Same CDS sequence) NM_182471.2 , NM_182471.3 , NM_182470.3 , NM_182470.2 , XM_017022313.1 , NM_182471.4 , XM_017022313.2
    Accession Version XM_017022313.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022313.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATAGCTCGTG AGGCTGAGGC AGCCATGTTC CACCGCAAGC TGTTTGAAGA ACTTGTGCGA
    GCCTCAAGTC ACTCCACAGA CCTCATGGAA GCCATGGCCA TGGGCAGCGT GGAGGCTTCT
    TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTGACGGAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877802.1
    CDS80..1675
    Translation

    Target ORF information:

    RefSeq Version XM_017022313.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022313.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATAGCTCGTG AGGCTGAGGC AGCCATGTTC CACCGCAAGC TGTTTGAAGA ACTTGTGCGA
    GCCTCAAGTC ACTCCACAGA CCTCATGGAA GCCATGGCCA TGGGCAGCGT GGAGGCTTCT
    TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTGACGGAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20299
    Clone ID Related Accession (Same CDS sequence) XM_005254445.5 , XM_005254445.3 , NM_002654.5 , NM_002654.4 , XM_005254445.4 , NM_002654.6
    Accession Version XM_005254445.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005254445.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATTGCCCGTG AGGCAGAGGC TGCCATCTAC CACTTGCAAT TATTTGAGGA ACTCCGCCGC
    CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCCACCGCCG TGGGTGCCGT GGAGGCCTCC
    TTCAAGTGCT GCAGTGGGGC CATAATCGTC CTCACCAAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005254502.1
    CDS80..1675
    Translation

    Target ORF information:

    RefSeq Version XM_005254445.5
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005254445.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATTGCCCGTG AGGCAGAGGC TGCCATCTAC CACTTGCAAT TATTTGAGGA ACTCCGCCGC
    CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCCACCGCCG TGGGTGCCGT GGAGGCCTCC
    TTCAAGTGCT GCAGTGGGGC CATAATCGTC CTCACCAAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20299
    Clone ID Related Accession (Same CDS sequence) XM_005254445.5 , XM_005254445.3 , NM_002654.5 , NM_002654.4 , XM_005254445.4 , NM_002654.6
    Accession Version XM_005254445.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product pyruvate kinase PKM isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATTGCCCGTG AGGCAGAGGC TGCCATCTAC CACTTGCAAT TATTTGAGGA ACTCCGCCGC
    CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCCACCGCCG TGGGTGCCGT GGAGGCCTCC
    TTCAAGTGCT GCAGTGGGGC CATAATCGTC CTCACCAAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005254502.1
    Translation

    Target ORF information:

    RefSeq Version XM_005254445.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens pyruvate kinase, muscle (PKM), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005254445.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATTGCCCGTG AGGCAGAGGC TGCCATCTAC CACTTGCAAT TATTTGAGGA ACTCCGCCGC
    CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCCACCGCCG TGGGTGCCGT GGAGGCCTCC
    TTCAAGTGCT GCAGTGGGGC CATAATCGTC CTCACCAAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20299
    Clone ID Related Accession (Same CDS sequence) XM_005254445.5 , XM_005254445.3 , NM_002654.5 , NM_002654.4 , XM_005254445.4 , NM_002654.6
    Accession Version XM_005254445.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254445.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATTGCCCGTG AGGCAGAGGC TGCCATCTAC CACTTGCAAT TATTTGAGGA ACTCCGCCGC
    CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCCACCGCCG TGGGTGCCGT GGAGGCCTCC
    TTCAAGTGCT GCAGTGGGGC CATAATCGTC CTCACCAAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005254502.1
    CDS84..1679
    Translation

    Target ORF information:

    RefSeq Version XM_005254445.4
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase, muscle (PKM), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005254445.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATTGCCCGTG AGGCAGAGGC TGCCATCTAC CACTTGCAAT TATTTGAGGA ACTCCGCCGC
    CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCCACCGCCG TGGGTGCCGT GGAGGCCTCC
    TTCAAGTGCT GCAGTGGGGC CATAATCGTC CTCACCAAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19800
    Clone ID Related Accession (Same CDS sequence) NM_001206797.2
    Accession Version NM_001206797.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC326232.1, AC020779.10, DC376054.1, AK297951.1, BC000481.2 and BC035198.1. On Oct 14, 2015 this sequence version replaced NM_001206797.1. Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence, lacks an alternate in-frame segment, and has an alternate in-frame coding exon compared to variant 4. The resulting isoform (d) is shorter at the N-terminus, lacks an alternate internal segment, and has a different internal segment compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297951.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA2145893 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGCTG
    AAGAAGGGAG CCACTCTCAA AATCACGCTG GATAACGCCT ACATGGAAAA GTGTGACGAG
    AACATCCTGT GGCTGGACTA CAAGAACATC TGCAAGGTGG TGGAAGTGGG CAGCAAGATC
    TACGTGGATG ATGGGCTTAT TTCTCTCCAG GTGAAGCAGA AAGGTGCCGA CTTCCTGGTG
    ACGGAGGTGG AAAATGGTGG CTCCTTGGGC AGCAAGAAGG GTGTGAACCT TCCTGGGGCT
    GCTGTGGACT TGCCTGCTGT GTCGGAGAAG GACATCCAGG ATCTGAAGTT TGGGGTCGAG
    CAGGATGTTG ATATGGTGTT TGCGTCATTC ATCCGCAAGG CATCTGATGT CCATGAAGTT
    AGGAAGGTCC TGGGAGAGAA GGGAAAGAAC ATCAAGATTA TCAGCAAAAT CGAGAATCAT
    GAGGGGGTTC GGAGGTTTGA TGAAATCCTG GAGGCCAGTG ATGGGATCAT GGTGGCTCGT
    GGTGATCTAG GCATTGAGAT TCCTGCAGAG AAGGTCTTCC TTGCTCAGAA GATGATGATT
    GGACGGTGCA ACCGAGCTGG GAAGCCTGTC ATCTGTGCTA CTCAGATGCT GGAGAGCATG
    ATCAAGAAGC CCCGCCCCAC TCGGGCTGAA GGCAGTGATG TGGCCAATGC AGTCCTGGAT
    GGAGCCGACT GCATCATGCT GTCTGGAGAA ACAGCCAAAG GGGACTATCC TCTGGAGGCT
    GTGCGCATGC AGCACCTGAT TGCCCGTGAG GCAGAGGCTG CCATCTACCA CTTGCAATTA
    TTTGAGGAAC TCCGCCGCCT GGCGCCCATT ACCAGCGACC CCACAGAAGC CACCGCCGTG
    GGTGCCGTGG AGGCCTCCTT CAAGTGCTGC AGTGGGGCCA TAATCGTCCT CACCAAGTCT
    GGCAGGTCTG CTCACCAGGT GGCCAGATAC CGCCCACGTG CCCCCATCAT TGCTGTGACC
    CGGAATCCCC AGACAGCTCG TCAGGCCCAC CTGTACCGTG GCATCTTCCC TGTGCTGTGC
    AAGGACCCAG TCCAGGAGGC CTGGGCTGAG GACGTGGACC TCCGGGTGAA CTTTGCCATG
    AATGTTGGCA AGGCCCGAGG CTTCTTCAAG AAGGGAGATG TGGTCATTGT GCTGACCGGA
    TGGCGCCCTG GCTCCGGCTT CACCAACACC ATGCGTGTTG TTCCTGTGCC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193726.1
    CDS523..1896
    Translation

    Target ORF information:

    RefSeq Version NM_001206797.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206797.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA 
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGCTG
    AAGAAGGGAG CCACTCTCAA AATCACGCTG GATAACGCCT ACATGGAAAA GTGTGACGAG
    AACATCCTGT GGCTGGACTA CAAGAACATC TGCAAGGTGG TGGAAGTGGG CAGCAAGATC
    TACGTGGATG ATGGGCTTAT TTCTCTCCAG GTGAAGCAGA AAGGTGCCGA CTTCCTGGTG
    ACGGAGGTGG AAAATGGTGG CTCCTTGGGC AGCAAGAAGG GTGTGAACCT TCCTGGGGCT
    GCTGTGGACT TGCCTGCTGT GTCGGAGAAG GACATCCAGG ATCTGAAGTT TGGGGTCGAG
    CAGGATGTTG ATATGGTGTT TGCGTCATTC ATCCGCAAGG CATCTGATGT CCATGAAGTT
    AGGAAGGTCC TGGGAGAGAA GGGAAAGAAC ATCAAGATTA TCAGCAAAAT CGAGAATCAT
    GAGGGGGTTC GGAGGTTTGA TGAAATCCTG GAGGCCAGTG ATGGGATCAT GGTGGCTCGT
    GGTGATCTAG GCATTGAGAT TCCTGCAGAG AAGGTCTTCC TTGCTCAGAA GATGATGATT
    GGACGGTGCA ACCGAGCTGG GAAGCCTGTC ATCTGTGCTA CTCAGATGCT GGAGAGCATG
    ATCAAGAAGC CCCGCCCCAC TCGGGCTGAA GGCAGTGATG TGGCCAATGC AGTCCTGGAT
    GGAGCCGACT GCATCATGCT GTCTGGAGAA ACAGCCAAAG GGGACTATCC TCTGGAGGCT
    GTGCGCATGC AGCACCTGAT TGCCCGTGAG GCAGAGGCTG CCATCTACCA CTTGCAATTA
    TTTGAGGAAC TCCGCCGCCT GGCGCCCATT ACCAGCGACC CCACAGAAGC CACCGCCGTG
    GGTGCCGTGG AGGCCTCCTT CAAGTGCTGC AGTGGGGCCA TAATCGTCCT CACCAAGTCT
    GGCAGGTCTG CTCACCAGGT GGCCAGATAC CGCCCACGTG CCCCCATCAT TGCTGTGACC
    CGGAATCCCC AGACAGCTCG TCAGGCCCAC CTGTACCGTG GCATCTTCCC TGTGCTGTGC
    AAGGACCCAG TCCAGGAGGC CTGGGCTGAG GACGTGGACC TCCGGGTGAA CTTTGCCATG
    AATGTTGGCA AGGCCCGAGG CTTCTTCAAG AAGGGAGATG TGGTCATTGT GCTGACCGGA
    TGGCGCCCTG GCTCCGGCTT CACCAACACC ATGCGTGTTG TTCCTGTGCC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20044
    Clone ID Related Accession (Same CDS sequence) NM_001206798.2
    Accession Version NM_001206798.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC326232.1, AC020779.10, DC376054.1, AK300800.1, BC000481.2 and BC035198.1. On Oct 14, 2015 this sequence version replaced NM_001206798.1. Transcript Variant: This variant (6) differs in the 5' UTR and coding sequence, lacks an alternate exon, and has an alternate in-frame coding exon compared to variant 4. The resulting isoform (e) has a shorter and distinct N-terminus, lacks an alternate internal segment, and has a different internal segment compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300800.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145893, SAMN01820691 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCACCGG AAGCCCAACC CCAGAGAACC AAAGGACCTC AGCAGCCATG TCGAAGCCCC 
    ATAGTGAAGC CGGGACTGCC TTCATTCAGA CCCAGCAGCT GCACGCAGCC ATGGCTGACA
    CATTCCTGGA GCACATGTGC CGCCTGGACA TTGATTCACC ACCCATCACA GCCCGGAACA
    CTGGCATCAT CTGTACCATT GTACCATGCG GAGACCATCA AGAATGTGCG CACAGCCACG
    GAAAGCTTTG CTTCTGACCC CATCCTCTAC CGGCCCGTTG CTGTGGCTCT AGACACTAAA
    GGACCTGAGA TCCGAACTGG GCTCATCAAG GGCAGCGGCA CTGCAGAGGT GGAGCTGAAG
    AAGGGAGCCA CTCTCAAAAT CACGCTGGAT AACGCCTACA TGGAAAAGTG TGACGAGAAC
    ATCCTGTGGC TGGACTACAA GAACATCTGC AAGGTGGTGG AAGTGGGCAG CAAGATCTAC
    GTGGATGATG GGCTTATTTC TCTCCAGGTG AAGCAGAAAG GTGCCGACTT CCTGGTGACG
    GAGGTGGAAA ATGGTGGCTC CTTGGGCAGC AAGAAGGGTG TGAACCTTCC TGGGGCTGCT
    GTGGACTTGC CTGCTGTGTC GGAGAAGGAC ATCCAGGATC TGAAGTTTGG GGTCGAGCAG
    GATGTTGATA TGGTGTTTGC GTCATTCATC CGCAAGGCAT CTGATGTCCA TGAAGTTAGG
    AAGGTCCTGG GAGAGAAGGG AAAGAACATC AAGATTATCA GCAAAATCGA GAATCATGAG
    GGGGTTCGGA GGTTTGATGA AATCCTGGAG GCCAGTGATG GGATCATGGT GGCTCGTGGT
    GATCTAGGCA TTGAGATTCC TGCAGAGAAG GTCTTCCTTG CTCAGAAGAT GATGATTGGA
    CGGTGCAACC GAGCTGGGAA GCCTGTCATC TGTGCTACTC AGATGCTGGA GAGCATGATC
    AAGAAGCCCC GCCCCACTCG GGCTGAAGGC AGTGATGTGG CCAATGCAGT CCTGGATGGA
    GCCGACTGCA TCATGCTGTC TGGAGAAACA GCCAAAGGGG ACTATCCTCT GGAGGCTGTG
    CGCATGCAGC ACCTGATTGC CCGTGAGGCA GAGGCTGCCA TCTACCACTT GCAATTATTT
    GAGGAACTCC GCCGCCTGGC GCCCATTACC AGCGACCCCA CAGAAGCCAC CGCCGTGGGT
    GCCGTGGAGG CCTCCTTCAA GTGCTGCAGT GGGGCCATAA TCGTCCTCAC CAAGTCTGGC
    AGGTCTGCTC ACCAGGTGGC CAGATACCGC CCACGTGCCC CCATCATTGC TGTGACCCGG
    AATCCCCAGA CAGCTCGTCA GGCCCACCTG TACCGTGGCA TCTTCCCTGT GCTGTGCAAG
    GACCCAGTCC AGGAGGCCTG GGCTGAGGAC GTGGACCTCC GGGTGAACTT TGCCATGAAT
    GTTGGCAAGG CCCGAGGCTT CTTCAAGAAG GGAGATGTGG TCATTGTGCT GACCGGATGG
    CGCCCTGGCT CCGGCTTCAC CAACACCATG CGTGTTGTTC CTGTGCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193727.1
    CDS593..2143
    Translation

    Target ORF information:

    RefSeq Version NM_001206798.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206798.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCACCGG AAGCCCAACC CCAGAGAACC AAAGGACCTC AGCAGCCATG TCGAAGCCCC 
    ATAGTGAAGC CGGGACTGCC TTCATTCAGA CCCAGCAGCT GCACGCAGCC ATGGCTGACA
    CATTCCTGGA GCACATGTGC CGCCTGGACA TTGATTCACC ACCCATCACA GCCCGGAACA
    CTGGCATCAT CTGTACCATT GTACCATGCG GAGACCATCA AGAATGTGCG CACAGCCACG
    GAAAGCTTTG CTTCTGACCC CATCCTCTAC CGGCCCGTTG CTGTGGCTCT AGACACTAAA
    GGACCTGAGA TCCGAACTGG GCTCATCAAG GGCAGCGGCA CTGCAGAGGT GGAGCTGAAG
    AAGGGAGCCA CTCTCAAAAT CACGCTGGAT AACGCCTACA TGGAAAAGTG TGACGAGAAC
    ATCCTGTGGC TGGACTACAA GAACATCTGC AAGGTGGTGG AAGTGGGCAG CAAGATCTAC
    GTGGATGATG GGCTTATTTC TCTCCAGGTG AAGCAGAAAG GTGCCGACTT CCTGGTGACG
    GAGGTGGAAA ATGGTGGCTC CTTGGGCAGC AAGAAGGGTG TGAACCTTCC TGGGGCTGCT
    GTGGACTTGC CTGCTGTGTC GGAGAAGGAC ATCCAGGATC TGAAGTTTGG GGTCGAGCAG
    GATGTTGATA TGGTGTTTGC GTCATTCATC CGCAAGGCAT CTGATGTCCA TGAAGTTAGG
    AAGGTCCTGG GAGAGAAGGG AAAGAACATC AAGATTATCA GCAAAATCGA GAATCATGAG
    GGGGTTCGGA GGTTTGATGA AATCCTGGAG GCCAGTGATG GGATCATGGT GGCTCGTGGT
    GATCTAGGCA TTGAGATTCC TGCAGAGAAG GTCTTCCTTG CTCAGAAGAT GATGATTGGA
    CGGTGCAACC GAGCTGGGAA GCCTGTCATC TGTGCTACTC AGATGCTGGA GAGCATGATC
    AAGAAGCCCC GCCCCACTCG GGCTGAAGGC AGTGATGTGG CCAATGCAGT CCTGGATGGA
    GCCGACTGCA TCATGCTGTC TGGAGAAACA GCCAAAGGGG ACTATCCTCT GGAGGCTGTG
    CGCATGCAGC ACCTGATTGC CCGTGAGGCA GAGGCTGCCA TCTACCACTT GCAATTATTT
    GAGGAACTCC GCCGCCTGGC GCCCATTACC AGCGACCCCA CAGAAGCCAC CGCCGTGGGT
    GCCGTGGAGG CCTCCTTCAA GTGCTGCAGT GGGGCCATAA TCGTCCTCAC CAAGTCTGGC
    AGGTCTGCTC ACCAGGTGGC CAGATACCGC CCACGTGCCC CCATCATTGC TGTGACCCGG
    AATCCCCAGA CAGCTCGTCA GGCCCACCTG TACCGTGGCA TCTTCCCTGT GCTGTGCAAG
    GACCCAGTCC AGGAGGCCTG GGCTGAGGAC GTGGACCTCC GGGTGAACTT TGCCATGAAT
    GTTGGCAAGG CCCGAGGCTT CTTCAAGAAG GGAGATGTGG TCATTGTGCT GACCGGATGG
    CGCCCTGGCT CCGGCTTCAC CAACACCATG CGTGTTGTTC CTGTGCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20230
    Clone ID Related Accession (Same CDS sequence) NM_001206799.2 , NM_001206799.1
    Accession Version NM_001206799.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020779.10, AK309781.1, AK294315.1, BC000481.2 and BC035198.1. On May 31, 2019 this sequence version replaced NM_001206799.1. Transcript Variant: This variant (7) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (f) has a shorter and distinct N-terminus compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK309781.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGACCTCAG CAGCCATGTC GAAGCCCCAT AGTGAAGCCG GGACTGCCTT CATTCAGACC 
    CAGCAGCTGC ACGCAGCCAT GGCTGACACA TTCCTGGAGC ACATGTGCCG CCTGGACATT
    GATTCACCAC CCATCACAGC CCGGAACACT GGCATCATCT GTACCATTGG CCCAGCTTCC
    CGATCAGTGG AGACGTTGAA GGAGATGATT AAGTCTGGAA TGAATGTGGC TCGTCTGAAC
    TTCTCTCATG GAACTCATGA GTACCATGCG GAGACCATCA AGAATGTGCG CACAGCCACG
    GAAAGCTTTG CTTCTGACCC CATCCTCTAC CGGCCCGTTG CTGTGGCTCT AGACACTAAA
    GGACCTGAGA TCCGAACTGG GCTCATCAAG GGCAGCGGCA CTGCAGAGGT GGAGCTGAAG
    AAGGGAGCCA CTCTCAAAAT CACGCTGGAT AACGCCTACA TGGAAAAGTG TGACGAGAAC
    ATCCTGTGGC TGGACTACAA GAACATCTGC AAGGTGGTGG AAGTGGGCAG CAAGATCTAC
    GTGGATGATG GGCTTATTTC TCTCCAGGTG AAGCAGAAAG GTGCCGACTT CCTGGTGACG
    GAGGTGGAAA ATGGTGGCTC CTTGGGCAGC AAGAAGGGTG TGAACCTTCC TGGGGCTGCT
    GTGGACTTGC CTGCTGTGTC GGAGAAGGAC ATCCAGGATC TGAAGTTTGG GGTCGAGCAG
    GATGTTGATA TGGTGTTTGC GTCATTCATC CGCAAGGCAT CTGATGTCCA TGAAGTTAGG
    AAGGTCCTGG GAGAGAAGGG AAAGAACATC AAGATTATCA GCAAAATCGA GAATCATGAG
    GGGGTTCGGA GGTTTGATGA AATCCTGGAG GCCAGTGATG GGATCATGGT GGCTCGTGGT
    GATCTAGGCA TTGAGATTCC TGCAGAGAAG GTCTTCCTTG CTCAGAAGAT GATGATTGGA
    CGGTGCAACC GAGCTGGGAA GCCTGTCATC TGTGCTACTC AGATGCTGGA GAGCATGATC
    AAGAAGCCCC GCCCCACTCG GGCTGAAGGC AGTGATGTGG CCAATGCAGT CCTGGATGGA
    GCCGACTGCA TCATGCTGTC TGGAGAAACA GCCAAAGGGG ACTATCCTCT GGAGGCTGTG
    CGCATGCAGC ACCTGATAGC TCGTGAGGCT GAGGCAGCCA TGTTCCACCG CAAGCTGTTT
    GAAGAACTTG TGCGAGCCTC AAGTCACTCC ACAGACCTCA TGGAAGCCAT GGCCATGGGC
    AGCGTGGAGG CTTCTTATAA GTGTTTAGCA GCAGCTTTGA TAGTTCTGAC GGAGTCTGGC
    AGGTCTGCTC ACCAGGTGGC CAGATACCGC CCACGTGCCC CCATCATTGC TGTGACCCGG
    AATCCCCAGA CAGCTCGTCA GGCCCACCTG TACCGTGGCA TCTTCCCTGT GCTGTGCAAG
    GACCCAGTCC AGGAGGCCTG GGCTGAGGAC GTGGACCTCC GGGTGAACTT TGCCATGAAT
    GTTGGCAAGG CCCGAGGCTT CTTCAAGAAG GGAGATGTGG TCATTGTGCT GACCGGATGG
    CGCCCTGGCT CCGGCTTCAC CAACACCATG CGTGTTGTTC CTGTGCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193728.1
    CDS364..1974
    Translation

    Target ORF information:

    RefSeq Version NM_001206799.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206799.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGACCTCAG CAGCCATGTC GAAGCCCCAT AGTGAAGCCG GGACTGCCTT CATTCAGACC 
    CAGCAGCTGC ACGCAGCCAT GGCTGACACA TTCCTGGAGC ACATGTGCCG CCTGGACATT
    GATTCACCAC CCATCACAGC CCGGAACACT GGCATCATCT GTACCATTGG CCCAGCTTCC
    CGATCAGTGG AGACGTTGAA GGAGATGATT AAGTCTGGAA TGAATGTGGC TCGTCTGAAC
    TTCTCTCATG GAACTCATGA GTACCATGCG GAGACCATCA AGAATGTGCG CACAGCCACG
    GAAAGCTTTG CTTCTGACCC CATCCTCTAC CGGCCCGTTG CTGTGGCTCT AGACACTAAA
    GGACCTGAGA TCCGAACTGG GCTCATCAAG GGCAGCGGCA CTGCAGAGGT GGAGCTGAAG
    AAGGGAGCCA CTCTCAAAAT CACGCTGGAT AACGCCTACA TGGAAAAGTG TGACGAGAAC
    ATCCTGTGGC TGGACTACAA GAACATCTGC AAGGTGGTGG AAGTGGGCAG CAAGATCTAC
    GTGGATGATG GGCTTATTTC TCTCCAGGTG AAGCAGAAAG GTGCCGACTT CCTGGTGACG
    GAGGTGGAAA ATGGTGGCTC CTTGGGCAGC AAGAAGGGTG TGAACCTTCC TGGGGCTGCT
    GTGGACTTGC CTGCTGTGTC GGAGAAGGAC ATCCAGGATC TGAAGTTTGG GGTCGAGCAG
    GATGTTGATA TGGTGTTTGC GTCATTCATC CGCAAGGCAT CTGATGTCCA TGAAGTTAGG
    AAGGTCCTGG GAGAGAAGGG AAAGAACATC AAGATTATCA GCAAAATCGA GAATCATGAG
    GGGGTTCGGA GGTTTGATGA AATCCTGGAG GCCAGTGATG GGATCATGGT GGCTCGTGGT
    GATCTAGGCA TTGAGATTCC TGCAGAGAAG GTCTTCCTTG CTCAGAAGAT GATGATTGGA
    CGGTGCAACC GAGCTGGGAA GCCTGTCATC TGTGCTACTC AGATGCTGGA GAGCATGATC
    AAGAAGCCCC GCCCCACTCG GGCTGAAGGC AGTGATGTGG CCAATGCAGT CCTGGATGGA
    GCCGACTGCA TCATGCTGTC TGGAGAAACA GCCAAAGGGG ACTATCCTCT GGAGGCTGTG
    CGCATGCAGC ACCTGATAGC TCGTGAGGCT GAGGCAGCCA TGTTCCACCG CAAGCTGTTT
    GAAGAACTTG TGCGAGCCTC AAGTCACTCC ACAGACCTCA TGGAAGCCAT GGCCATGGGC
    AGCGTGGAGG CTTCTTATAA GTGTTTAGCA GCAGCTTTGA TAGTTCTGAC GGAGTCTGGC
    AGGTCTGCTC ACCAGGTGGC CAGATACCGC CCACGTGCCC CCATCATTGC TGTGACCCGG
    AATCCCCAGA CAGCTCGTCA GGCCCACCTG TACCGTGGCA TCTTCCCTGT GCTGTGCAAG
    GACCCAGTCC AGGAGGCCTG GGCTGAGGAC GTGGACCTCC GGGTGAACTT TGCCATGAAT
    GTTGGCAAGG CCCGAGGCTT CTTCAAGAAG GGAGATGTGG TCATTGTGCT GACCGGATGG
    CGCCCTGGCT CCGGCTTCAC CAACACCATG CGTGTTGTTC CTGTGCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20230
    Clone ID Related Accession (Same CDS sequence) NM_001206799.2 , NM_001206799.1
    Accession Version NM_001206799.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK309781.1, AK294315.1, BC000481.2 and BC035198.1. Transcript Variant: This variant (7) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (f) has a shorter and distinct N-terminus compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK309781.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGACCTCAG CAGCCATGTC GAAGCCCCAT AGTGAAGCCG GGACTGCCTT CATTCAGACC 
    CAGCAGCTGC ACGCAGCCAT GGCTGACACA TTCCTGGAGC ACATGTGCCG CCTGGACATT
    GATTCACCAC CCATCACAGC CCGGAACACT GGCATCATCT GTACCATTGG CCCAGCTTCC
    CGATCAGTGG AGACGTTGAA GGAGATGATT AAGTCTGGAA TGAATGTGGC TCGTCTGAAC
    TTCTCTCATG GAACTCATGA GTACCATGCG GAGACCATCA AGAATGTGCG CACAGCCACG
    GAAAGCTTTG CTTCTGACCC CATCCTCTAC CGGCCCGTTG CTGTGGCTCT AGACACTAAA
    GGACCTGAGA TCCGAACTGG GCTCATCAAG GGCAGCGGCA CTGCAGAGGT GGAGCTGAAG
    AAGGGAGCCA CTCTCAAAAT CACGCTGGAT AACGCCTACA TGGAAAAGTG TGACGAGAAC
    ATCCTGTGGC TGGACTACAA GAACATCTGC AAGGTGGTGG AAGTGGGCAG CAAGATCTAC
    GTGGATGATG GGCTTATTTC TCTCCAGGTG AAGCAGAAAG GTGCCGACTT CCTGGTGACG
    GAGGTGGAAA ATGGTGGCTC CTTGGGCAGC AAGAAGGGTG TGAACCTTCC TGGGGCTGCT
    GTGGACTTGC CTGCTGTGTC GGAGAAGGAC ATCCAGGATC TGAAGTTTGG GGTCGAGCAG
    GATGTTGATA TGGTGTTTGC GTCATTCATC CGCAAGGCAT CTGATGTCCA TGAAGTTAGG
    AAGGTCCTGG GAGAGAAGGG AAAGAACATC AAGATTATCA GCAAAATCGA GAATCATGAG
    GGGGTTCGGA GGTTTGATGA AATCCTGGAG GCCAGTGATG GGATCATGGT GGCTCGTGGT
    GATCTAGGCA TTGAGATTCC TGCAGAGAAG GTCTTCCTTG CTCAGAAGAT GATGATTGGA
    CGGTGCAACC GAGCTGGGAA GCCTGTCATC TGTGCTACTC AGATGCTGGA GAGCATGATC
    AAGAAGCCCC GCCCCACTCG GGCTGAAGGC AGTGATGTGG CCAATGCAGT CCTGGATGGA
    GCCGACTGCA TCATGCTGTC TGGAGAAACA GCCAAAGGGG ACTATCCTCT GGAGGCTGTG
    CGCATGCAGC ACCTGATAGC TCGTGAGGCT GAGGCAGCCA TGTTCCACCG CAAGCTGTTT
    GAAGAACTTG TGCGAGCCTC AAGTCACTCC ACAGACCTCA TGGAAGCCAT GGCCATGGGC
    AGCGTGGAGG CTTCTTATAA GTGTTTAGCA GCAGCTTTGA TAGTTCTGAC GGAGTCTGGC
    AGGTCTGCTC ACCAGGTGGC CAGATACCGC CCACGTGCCC CCATCATTGC TGTGACCCGG
    AATCCCCAGA CAGCTCGTCA GGCCCACCTG TACCGTGGCA TCTTCCCTGT GCTGTGCAAG
    GACCCAGTCC AGGAGGCCTG GGCTGAGGAC GTGGACCTCC GGGTGAACTT TGCCATGAAT
    GTTGGCAAGG CCCGAGGCTT CTTCAAGAAG GGAGATGTGG TCATTGTGCT GACCGGATGG
    CGCCCTGGCT CCGGCTTCAC CAACACCATG CGTGTTGTTC CTGTGCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193728.1
    CDS175..1785
    Translation

    Target ORF information:

    RefSeq Version NM_001206799.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206799.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGACCTCAG CAGCCATGTC GAAGCCCCAT AGTGAAGCCG GGACTGCCTT CATTCAGACC 
    CAGCAGCTGC ACGCAGCCAT GGCTGACACA TTCCTGGAGC ACATGTGCCG CCTGGACATT
    GATTCACCAC CCATCACAGC CCGGAACACT GGCATCATCT GTACCATTGG CCCAGCTTCC
    CGATCAGTGG AGACGTTGAA GGAGATGATT AAGTCTGGAA TGAATGTGGC TCGTCTGAAC
    TTCTCTCATG GAACTCATGA GTACCATGCG GAGACCATCA AGAATGTGCG CACAGCCACG
    GAAAGCTTTG CTTCTGACCC CATCCTCTAC CGGCCCGTTG CTGTGGCTCT AGACACTAAA
    GGACCTGAGA TCCGAACTGG GCTCATCAAG GGCAGCGGCA CTGCAGAGGT GGAGCTGAAG
    AAGGGAGCCA CTCTCAAAAT CACGCTGGAT AACGCCTACA TGGAAAAGTG TGACGAGAAC
    ATCCTGTGGC TGGACTACAA GAACATCTGC AAGGTGGTGG AAGTGGGCAG CAAGATCTAC
    GTGGATGATG GGCTTATTTC TCTCCAGGTG AAGCAGAAAG GTGCCGACTT CCTGGTGACG
    GAGGTGGAAA ATGGTGGCTC CTTGGGCAGC AAGAAGGGTG TGAACCTTCC TGGGGCTGCT
    GTGGACTTGC CTGCTGTGTC GGAGAAGGAC ATCCAGGATC TGAAGTTTGG GGTCGAGCAG
    GATGTTGATA TGGTGTTTGC GTCATTCATC CGCAAGGCAT CTGATGTCCA TGAAGTTAGG
    AAGGTCCTGG GAGAGAAGGG AAAGAACATC AAGATTATCA GCAAAATCGA GAATCATGAG
    GGGGTTCGGA GGTTTGATGA AATCCTGGAG GCCAGTGATG GGATCATGGT GGCTCGTGGT
    GATCTAGGCA TTGAGATTCC TGCAGAGAAG GTCTTCCTTG CTCAGAAGAT GATGATTGGA
    CGGTGCAACC GAGCTGGGAA GCCTGTCATC TGTGCTACTC AGATGCTGGA GAGCATGATC
    AAGAAGCCCC GCCCCACTCG GGCTGAAGGC AGTGATGTGG CCAATGCAGT CCTGGATGGA
    GCCGACTGCA TCATGCTGTC TGGAGAAACA GCCAAAGGGG ACTATCCTCT GGAGGCTGTG
    CGCATGCAGC ACCTGATAGC TCGTGAGGCT GAGGCAGCCA TGTTCCACCG CAAGCTGTTT
    GAAGAACTTG TGCGAGCCTC AAGTCACTCC ACAGACCTCA TGGAAGCCAT GGCCATGGGC
    AGCGTGGAGG CTTCTTATAA GTGTTTAGCA GCAGCTTTGA TAGTTCTGAC GGAGTCTGGC
    AGGTCTGCTC ACCAGGTGGC CAGATACCGC CCACGTGCCC CCATCATTGC TGTGACCCGG
    AATCCCCAGA CAGCTCGTCA GGCCCACCTG TACCGTGGCA TCTTCCCTGT GCTGTGCAAG
    GACCCAGTCC AGGAGGCCTG GGCTGAGGAC GTGGACCTCC GGGTGAACTT TGCCATGAAT
    GTTGGCAAGG CCCGAGGCTT CTTCAAGAAG GGAGATGTGG TCATTGTGCT GACCGGATGG
    CGCCCTGGCT CCGGCTTCAC CAACACCATG CGTGTTGTTC CTGTGCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59743
    Clone ID Related Accession (Same CDS sequence) NM_001316318.2 , NM_001316318.1
    Accession Version NM_001316318.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform g
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020779.10. On May 31, 2019 this sequence version replaced NM_001316318.1. Transcript Variant: This variant (8) lacks an alternate in-frame exon in the 5' end of the coding sequence compared to variant 4. The resulting isoform (g) has the same N- and C-termini but is shorter compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1124222.1, BC096823.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCAGTGGA GCTCAGAGAG AGGAGAACGG CTCCTCACGC CTGGGGCCTG CTCTTCAGAA 
    GTCCCCAGCG CCGTTCCTTC CAGATCAGGC GGGACCTCAG CAGCCATGTC GAAGCCCCAT
    AGTGAAGCCG GGACTGCCTT CATTCAGACC CAGCAGCTGC ACGCAGCCAT GGCTGACACA
    TTCCTGGAGC ACATGTGCCG CCTGGACATT GATTCACCAC CCATCACAGC CCGGAACACT
    GGCATCATCT GTACCATTGG CCCAGCTTCC CGATCAGTGG AGACGTTGAA GGAGATGATT
    AAGTCTGGAA TGAATGTGGC TCGTCTGAAC TTCTCTCATG GAACTCATGA GTACCATGCG
    GAGACCATCA AGAATGTGCG CACAGCCACG GAAAGCTTTG CTTCTGACCC CATCCTCTAC
    CGGCCCGTTG CTGTGGCTCT AGACACTAAA GGACCTGAGA TCCGAACTGG GCTCATCAAG
    GGCAGCGGCA CTGCAGAGGT GGAGCTGAAG AAGGGAGCCA CTCTCAAAAT CACGCTGGAT
    AACGCCTACA TGGAAAAGTG TGACGAGAAC ATCCTGTGGC TGGACTACAA GAACATCTGC
    AAGGTGGTGG AAGTGGGCAG CAAGATCTAC GTGGATGATG GGCTTATTTC TCTCCAGGTG
    AAGCAGAAAG GTGCCGACTT CCTGGTGACG GAGGTGGAAA ATGGTGGCTC CTTGGGCAGC
    AAGAAGGGTG TGAACCTTCC TGGGGCTGCT GTGGACTTGC CTGCTGTGTC GGAGAAGGAC
    ATCCAGGATC TGAAGTTTGG GGTCGAGCAG GATGTTGATA TGGTGTTTGC GTCATTCATC
    CGCAAGGCAT CTGATGTCCA TGAAGTTAGG AAGGTCCTGG GAGAGAAGGG AAAGAACATC
    AAGATTATCA GCAAAATCGA GAATCATGAG GGGGTTCGGA GGTTTGATGA AATCCTGGAG
    GCCAGTGATG GGATCATGGT GGCTCGTGGT GATCTAGGCA TTGAGATTCC TGCAGAGAAG
    GTCTTCCTTG CTCAGAAGAT GATGATTGGA CGGTGCAACC GAGCTGGGAA GCCTGTCATC
    TGTGCTACTC AGATGCTGGA GAGCATGATC AAGAAGCCCC GCCCCACTCG GGCTGAAGGC
    AGTGATGTGG CCAATGCAGT CCTGGATGGA GCCGACTGCA TCATGCTGTC TGGAGAAACA
    GCCAAAGGGG ACTATCCTCT GGAGGCTGTG CGCATGCAGC ACCTGATAGC TCGTGAGGCT
    GAGGCAGCCA TGTTCCACCG CAAGCTGTTT GAAGAACTTG TGCGAGCCTC AAGTCACTCC
    ACAGACCTCA TGGAAGCCAT GGCCATGGGC AGCGTGGAGG CTTCTTATAA GTGTTTAGCA
    GCAGCTTTGA TAGTTCTGAC GGAGTCTGGC AGGTCTGCTC ACCAGGTGGC CAGATACCGC
    CCACGTGCCC CCATCATTGC TGTGACCCGG AATCCCCAGA CAGCTCGTCA GGCCCACCTG
    TACCGTGGCA TCTTCCCTGT GCTGTGCAAG GACCCAGTCC AGGAGGCCTG GGCTGAGGAC
    GTGGACCTCC GGGTGAACTT TGCCATGAAT GTTGGCAAGG CCCGAGGCTT CTTCAAGAAG
    GGAGATGTGG TCATTGTGCT GACCGGATGG CGCCCTGGCT CCGGCTTCAC CAACACCATG
    CGTGTTGTTC CTGTGCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001303247.1
    CDS204..1904
    Translation

    Target ORF information:

    RefSeq Version NM_001316318.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001316318.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCAGTGGA GCTCAGAGAG AGGAGAACGG CTCCTCACGC CTGGGGCCTG CTCTTCAGAA 
    GTCCCCAGCG CCGTTCCTTC CAGATCAGGC GGGACCTCAG CAGCCATGTC GAAGCCCCAT
    AGTGAAGCCG GGACTGCCTT CATTCAGACC CAGCAGCTGC ACGCAGCCAT GGCTGACACA
    TTCCTGGAGC ACATGTGCCG CCTGGACATT GATTCACCAC CCATCACAGC CCGGAACACT
    GGCATCATCT GTACCATTGG CCCAGCTTCC CGATCAGTGG AGACGTTGAA GGAGATGATT
    AAGTCTGGAA TGAATGTGGC TCGTCTGAAC TTCTCTCATG GAACTCATGA GTACCATGCG
    GAGACCATCA AGAATGTGCG CACAGCCACG GAAAGCTTTG CTTCTGACCC CATCCTCTAC
    CGGCCCGTTG CTGTGGCTCT AGACACTAAA GGACCTGAGA TCCGAACTGG GCTCATCAAG
    GGCAGCGGCA CTGCAGAGGT GGAGCTGAAG AAGGGAGCCA CTCTCAAAAT CACGCTGGAT
    AACGCCTACA TGGAAAAGTG TGACGAGAAC ATCCTGTGGC TGGACTACAA GAACATCTGC
    AAGGTGGTGG AAGTGGGCAG CAAGATCTAC GTGGATGATG GGCTTATTTC TCTCCAGGTG
    AAGCAGAAAG GTGCCGACTT CCTGGTGACG GAGGTGGAAA ATGGTGGCTC CTTGGGCAGC
    AAGAAGGGTG TGAACCTTCC TGGGGCTGCT GTGGACTTGC CTGCTGTGTC GGAGAAGGAC
    ATCCAGGATC TGAAGTTTGG GGTCGAGCAG GATGTTGATA TGGTGTTTGC GTCATTCATC
    CGCAAGGCAT CTGATGTCCA TGAAGTTAGG AAGGTCCTGG GAGAGAAGGG AAAGAACATC
    AAGATTATCA GCAAAATCGA GAATCATGAG GGGGTTCGGA GGTTTGATGA AATCCTGGAG
    GCCAGTGATG GGATCATGGT GGCTCGTGGT GATCTAGGCA TTGAGATTCC TGCAGAGAAG
    GTCTTCCTTG CTCAGAAGAT GATGATTGGA CGGTGCAACC GAGCTGGGAA GCCTGTCATC
    TGTGCTACTC AGATGCTGGA GAGCATGATC AAGAAGCCCC GCCCCACTCG GGCTGAAGGC
    AGTGATGTGG CCAATGCAGT CCTGGATGGA GCCGACTGCA TCATGCTGTC TGGAGAAACA
    GCCAAAGGGG ACTATCCTCT GGAGGCTGTG CGCATGCAGC ACCTGATAGC TCGTGAGGCT
    GAGGCAGCCA TGTTCCACCG CAAGCTGTTT GAAGAACTTG TGCGAGCCTC AAGTCACTCC
    ACAGACCTCA TGGAAGCCAT GGCCATGGGC AGCGTGGAGG CTTCTTATAA GTGTTTAGCA
    GCAGCTTTGA TAGTTCTGAC GGAGTCTGGC AGGTCTGCTC ACCAGGTGGC CAGATACCGC
    CCACGTGCCC CCATCATTGC TGTGACCCGG AATCCCCAGA CAGCTCGTCA GGCCCACCTG
    TACCGTGGCA TCTTCCCTGT GCTGTGCAAG GACCCAGTCC AGGAGGCCTG GGCTGAGGAC
    GTGGACCTCC GGGTGAACTT TGCCATGAAT GTTGGCAAGG CCCGAGGCTT CTTCAAGAAG
    GGAGATGTGG TCATTGTGCT GACCGGATGG CGCCCTGGCT CCGGCTTCAC CAACACCATG
    CGTGTTGTTC CTGTGCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59743
    Clone ID Related Accession (Same CDS sequence) NM_001316318.2 , NM_001316318.1
    Accession Version NM_001316318.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform g
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC326232.1, AC020779.10, DC376054.1, BC096823.1, AK294315.1, BC000481.2 and BC035198.1. On Oct 14, 2015 this sequence version replaced XM_011521671.1. Transcript Variant: This variant (8) lacks an alternate in-frame exon in the 5' end of the coding sequence compared to variant 4. The resulting isoform (g) has the same N- and C-termini but is shorter compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC096823.1, SRR1660805.115596.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCAGTGGA GCTCAGAGAG AGGAGAACGG CTCCTCACGC CTGGGGCCTG CTCTTCAGAA 
    GTCCCCAGCG CCGTTCCTTC CAGATCAGGC GGGACCTCAG CAGCCATGTC GAAGCCCCAT
    AGTGAAGCCG GGACTGCCTT CATTCAGACC CAGCAGCTGC ACGCAGCCAT GGCTGACACA
    TTCCTGGAGC ACATGTGCCG CCTGGACATT GATTCACCAC CCATCACAGC CCGGAACACT
    GGCATCATCT GTACCATTGG CCCAGCTTCC CGATCAGTGG AGACGTTGAA GGAGATGATT
    AAGTCTGGAA TGAATGTGGC TCGTCTGAAC TTCTCTCATG GAACTCATGA GTACCATGCG
    GAGACCATCA AGAATGTGCG CACAGCCACG GAAAGCTTTG CTTCTGACCC CATCCTCTAC
    CGGCCCGTTG CTGTGGCTCT AGACACTAAA GGACCTGAGA TCCGAACTGG GCTCATCAAG
    GGCAGCGGCA CTGCAGAGGT GGAGCTGAAG AAGGGAGCCA CTCTCAAAAT CACGCTGGAT
    AACGCCTACA TGGAAAAGTG TGACGAGAAC ATCCTGTGGC TGGACTACAA GAACATCTGC
    AAGGTGGTGG AAGTGGGCAG CAAGATCTAC GTGGATGATG GGCTTATTTC TCTCCAGGTG
    AAGCAGAAAG GTGCCGACTT CCTGGTGACG GAGGTGGAAA ATGGTGGCTC CTTGGGCAGC
    AAGAAGGGTG TGAACCTTCC TGGGGCTGCT GTGGACTTGC CTGCTGTGTC GGAGAAGGAC
    ATCCAGGATC TGAAGTTTGG GGTCGAGCAG GATGTTGATA TGGTGTTTGC GTCATTCATC
    CGCAAGGCAT CTGATGTCCA TGAAGTTAGG AAGGTCCTGG GAGAGAAGGG AAAGAACATC
    AAGATTATCA GCAAAATCGA GAATCATGAG GGGGTTCGGA GGTTTGATGA AATCCTGGAG
    GCCAGTGATG GGATCATGGT GGCTCGTGGT GATCTAGGCA TTGAGATTCC TGCAGAGAAG
    GTCTTCCTTG CTCAGAAGAT GATGATTGGA CGGTGCAACC GAGCTGGGAA GCCTGTCATC
    TGTGCTACTC AGATGCTGGA GAGCATGATC AAGAAGCCCC GCCCCACTCG GGCTGAAGGC
    AGTGATGTGG CCAATGCAGT CCTGGATGGA GCCGACTGCA TCATGCTGTC TGGAGAAACA
    GCCAAAGGGG ACTATCCTCT GGAGGCTGTG CGCATGCAGC ACCTGATAGC TCGTGAGGCT
    GAGGCAGCCA TGTTCCACCG CAAGCTGTTT GAAGAACTTG TGCGAGCCTC AAGTCACTCC
    ACAGACCTCA TGGAAGCCAT GGCCATGGGC AGCGTGGAGG CTTCTTATAA GTGTTTAGCA
    GCAGCTTTGA TAGTTCTGAC GGAGTCTGGC AGGTCTGCTC ACCAGGTGGC CAGATACCGC
    CCACGTGCCC CCATCATTGC TGTGACCCGG AATCCCCAGA CAGCTCGTCA GGCCCACCTG
    TACCGTGGCA TCTTCCCTGT GCTGTGCAAG GACCCAGTCC AGGAGGCCTG GGCTGAGGAC
    GTGGACCTCC GGGTGAACTT TGCCATGAAT GTTGGCAAGG CCCGAGGCTT CTTCAAGAAG
    GGAGATGTGG TCATTGTGCT GACCGGATGG CGCCCTGGCT CCGGCTTCAC CAACACCATG
    CGTGTTGTTC CTGTGCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001303247.1
    CDS638..2338
    Translation

    Target ORF information:

    RefSeq Version NM_001316318.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001316318.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCAGTGGA GCTCAGAGAG AGGAGAACGG CTCCTCACGC CTGGGGCCTG CTCTTCAGAA 
    GTCCCCAGCG CCGTTCCTTC CAGATCAGGC GGGACCTCAG CAGCCATGTC GAAGCCCCAT
    AGTGAAGCCG GGACTGCCTT CATTCAGACC CAGCAGCTGC ACGCAGCCAT GGCTGACACA
    TTCCTGGAGC ACATGTGCCG CCTGGACATT GATTCACCAC CCATCACAGC CCGGAACACT
    GGCATCATCT GTACCATTGG CCCAGCTTCC CGATCAGTGG AGACGTTGAA GGAGATGATT
    AAGTCTGGAA TGAATGTGGC TCGTCTGAAC TTCTCTCATG GAACTCATGA GTACCATGCG
    GAGACCATCA AGAATGTGCG CACAGCCACG GAAAGCTTTG CTTCTGACCC CATCCTCTAC
    CGGCCCGTTG CTGTGGCTCT AGACACTAAA GGACCTGAGA TCCGAACTGG GCTCATCAAG
    GGCAGCGGCA CTGCAGAGGT GGAGCTGAAG AAGGGAGCCA CTCTCAAAAT CACGCTGGAT
    AACGCCTACA TGGAAAAGTG TGACGAGAAC ATCCTGTGGC TGGACTACAA GAACATCTGC
    AAGGTGGTGG AAGTGGGCAG CAAGATCTAC GTGGATGATG GGCTTATTTC TCTCCAGGTG
    AAGCAGAAAG GTGCCGACTT CCTGGTGACG GAGGTGGAAA ATGGTGGCTC CTTGGGCAGC
    AAGAAGGGTG TGAACCTTCC TGGGGCTGCT GTGGACTTGC CTGCTGTGTC GGAGAAGGAC
    ATCCAGGATC TGAAGTTTGG GGTCGAGCAG GATGTTGATA TGGTGTTTGC GTCATTCATC
    CGCAAGGCAT CTGATGTCCA TGAAGTTAGG AAGGTCCTGG GAGAGAAGGG AAAGAACATC
    AAGATTATCA GCAAAATCGA GAATCATGAG GGGGTTCGGA GGTTTGATGA AATCCTGGAG
    GCCAGTGATG GGATCATGGT GGCTCGTGGT GATCTAGGCA TTGAGATTCC TGCAGAGAAG
    GTCTTCCTTG CTCAGAAGAT GATGATTGGA CGGTGCAACC GAGCTGGGAA GCCTGTCATC
    TGTGCTACTC AGATGCTGGA GAGCATGATC AAGAAGCCCC GCCCCACTCG GGCTGAAGGC
    AGTGATGTGG CCAATGCAGT CCTGGATGGA GCCGACTGCA TCATGCTGTC TGGAGAAACA
    GCCAAAGGGG ACTATCCTCT GGAGGCTGTG CGCATGCAGC ACCTGATAGC TCGTGAGGCT
    GAGGCAGCCA TGTTCCACCG CAAGCTGTTT GAAGAACTTG TGCGAGCCTC AAGTCACTCC
    ACAGACCTCA TGGAAGCCAT GGCCATGGGC AGCGTGGAGG CTTCTTATAA GTGTTTAGCA
    GCAGCTTTGA TAGTTCTGAC GGAGTCTGGC AGGTCTGCTC ACCAGGTGGC CAGATACCGC
    CCACGTGCCC CCATCATTGC TGTGACCCGG AATCCCCAGA CAGCTCGTCA GGCCCACCTG
    TACCGTGGCA TCTTCCCTGT GCTGTGCAAG GACCCAGTCC AGGAGGCCTG GGCTGAGGAC
    GTGGACCTCC GGGTGAACTT TGCCATGAAT GTTGGCAAGG CCCGAGGCTT CTTCAAGAAG
    GGAGATGTGG TCATTGTGCT GACCGGATGG CGCCCTGGCT CCGGCTTCAC CAACACCATG
    CGTGTTGTTC CTGTGCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48374
    Clone ID Related Accession (Same CDS sequence) XM_006720570.1
    Accession Version XM_006720570.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCAGTGGA GCTCAGAGAG AGGAGAACGG CTCCTCACGC CTGGGGCCTG CTCTTCAGAA 
    GTCCCCAGCG CCGTTCCTTC CAGATCAGGC GGCTCTCCAG GGCACACCGT ATTCAGCTCT
    GAGCGGTCTT TGCTAGTGAG GCCAAGGAGC CACCCTGAGC CAAAAGGGGA GCATTATGTC
    ACCGGAAGCC CAACCCCAGA GAACCAAAGG ACCTCAGCAG CCATGTCGAA GCCCCATAGT
    GAAGCCGGGA CTGCCTTCAT TCAGACCCAG CAGCTGCACG CAGCCATGGC TGACACATTC
    CTGGAGCACA TGTGCCGCCT GGACATTGAT TCACCACCCA TCACAGCCCG GAACACTGGC
    ATCATCTGTA CCATTGGCCC AGCTTCCCGA TCAGTGGAGA CGTTGAAGGA GATGATTAAG
    TCTGGAATGA ATGTGGCTCG TCTGAACTTC TCTCATGGAA CTCATGAGTA CCATGCGGAG
    ACCATCAAGA ATGTGCGCAC AGCCACGGAA AGCTTTGCTT CTGACCCCAT CCTCTACCGG
    CCCGTTGCTG TGGCTCTAGA CACTAAAGGA CCTGAGATCC GAACTGGGCT CATCAAGGGC
    AGCGGCACTG CAGAGGTGGA GCTGAAGAAG GGAGCCACTC TCAAAATCAC GCTGGATAAC
    GCCTACATGG AAAAGTGTGA CGAGAACATC CTGTGGCTGG ACTACAAGAA CATCTGCAAG
    GTGGTGGAAG TGGGCAGCAA GATCTACGTG GATGATGGGC TTATTTCTCT CCAGGTGAAG
    CAGAAAGGTG CCGACTTCCT GGTGACGGAG GTGGAAAATG GTGGCTCCTT GGGCAGCAAG
    AAGGGTGTGA ACCTTCCTGG GGCTGCTGTG GACTTGCCTG CTGTGTCGGA GAAGGACATC
    CAGGATCTGA AGTTTGGGGT CGAGCAGGAT GTTGATATGG TGTTTGCGTC ATTCATCCGC
    AAGGCATCTG ATGTCCATGA AGTTAGGAAG GTCCTGGGAG AGAAGGGAAA GAACATCAAG
    ATTATCAGCA AAATCGAGAA TCATGAGGGG GTTCGGAGGT TTGATGAAAT CCTGGAGGCC
    AGTGATGGGA TCATGGTGGC TCGTGGTGAT CTAGGCATTG AGATTCCTGC AGAGAAGGTC
    TTCCTTGCTC AGAAGATGAT GATTGGACGG TGCAACCGAG CTGGGAAGCC TGTCATCTGT
    GCTACTCAGA TGCTGGAGAG CATGATCAAG AAGCCCCGCC CCACTCGGGC TGAAGGCAGT
    GATGTGGCCA ATGCAGTCCT GGATGGAGCC GACTGCATCA TGCTGTCTGG AGAAACAGCC
    AAAGGGGACT ATCCTCTGGA GGCTGTGCGC ATGCAGCACC TGATTGCCCG TGAGGCAGAG
    GCTGCCATCT ACCACTTGCA ATTATTTGAG GAACTCCGCC GCCTGGCGCC CATTACCAGC
    GACCCCACAG AAGCCACCGC CGTGGGTGCC GTGGAGGCCT CCTTCAAGTG CTGCAGTGGG
    GCCATAATCG TCCTCACCAA GTCTGGCAGG TCTGCTCACC AGGTGGCCAG ATACCGCCCA
    CGTGCCCCCA TCATTGCTGT GACCCGGAAT CCCCAGACAG CTCGTCAGGC CCACCTGTAC
    CGTGGCATCT TCCCTGTGCT GTGCAAGGAC CCAGTCCAGG AGGCCTGGGC TGAGGACGTG
    GACCTCCGGG TGAACTTTGC CATGAATGTT GGCAAGGCCC GAGGCTTCTT CAAGAAGGGA
    GATGTGGTCA TTGTGCTGAC CGGATGGCGC CCTGGCTCCG GCTTCACCAA CACCATGCGT
    GTTGTTCCTG TGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720633.1
    CDS122..1939
    Translation

    Target ORF information:

    RefSeq Version XM_006720570.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720570.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCAGTGGA GCTCAGAGAG AGGAGAACGG CTCCTCACGC CTGGGGCCTG CTCTTCAGAA 
    GTCCCCAGCG CCGTTCCTTC CAGATCAGGC GGCTCTCCAG GGCACACCGT ATTCAGCTCT
    GAGCGGTCTT TGCTAGTGAG GCCAAGGAGC CACCCTGAGC CAAAAGGGGA GCATTATGTC
    ACCGGAAGCC CAACCCCAGA GAACCAAAGG ACCTCAGCAG CCATGTCGAA GCCCCATAGT
    GAAGCCGGGA CTGCCTTCAT TCAGACCCAG CAGCTGCACG CAGCCATGGC TGACACATTC
    CTGGAGCACA TGTGCCGCCT GGACATTGAT TCACCACCCA TCACAGCCCG GAACACTGGC
    ATCATCTGTA CCATTGGCCC AGCTTCCCGA TCAGTGGAGA CGTTGAAGGA GATGATTAAG
    TCTGGAATGA ATGTGGCTCG TCTGAACTTC TCTCATGGAA CTCATGAGTA CCATGCGGAG
    ACCATCAAGA ATGTGCGCAC AGCCACGGAA AGCTTTGCTT CTGACCCCAT CCTCTACCGG
    CCCGTTGCTG TGGCTCTAGA CACTAAAGGA CCTGAGATCC GAACTGGGCT CATCAAGGGC
    AGCGGCACTG CAGAGGTGGA GCTGAAGAAG GGAGCCACTC TCAAAATCAC GCTGGATAAC
    GCCTACATGG AAAAGTGTGA CGAGAACATC CTGTGGCTGG ACTACAAGAA CATCTGCAAG
    GTGGTGGAAG TGGGCAGCAA GATCTACGTG GATGATGGGC TTATTTCTCT CCAGGTGAAG
    CAGAAAGGTG CCGACTTCCT GGTGACGGAG GTGGAAAATG GTGGCTCCTT GGGCAGCAAG
    AAGGGTGTGA ACCTTCCTGG GGCTGCTGTG GACTTGCCTG CTGTGTCGGA GAAGGACATC
    CAGGATCTGA AGTTTGGGGT CGAGCAGGAT GTTGATATGG TGTTTGCGTC ATTCATCCGC
    AAGGCATCTG ATGTCCATGA AGTTAGGAAG GTCCTGGGAG AGAAGGGAAA GAACATCAAG
    ATTATCAGCA AAATCGAGAA TCATGAGGGG GTTCGGAGGT TTGATGAAAT CCTGGAGGCC
    AGTGATGGGA TCATGGTGGC TCGTGGTGAT CTAGGCATTG AGATTCCTGC AGAGAAGGTC
    TTCCTTGCTC AGAAGATGAT GATTGGACGG TGCAACCGAG CTGGGAAGCC TGTCATCTGT
    GCTACTCAGA TGCTGGAGAG CATGATCAAG AAGCCCCGCC CCACTCGGGC TGAAGGCAGT
    GATGTGGCCA ATGCAGTCCT GGATGGAGCC GACTGCATCA TGCTGTCTGG AGAAACAGCC
    AAAGGGGACT ATCCTCTGGA GGCTGTGCGC ATGCAGCACC TGATTGCCCG TGAGGCAGAG
    GCTGCCATCT ACCACTTGCA ATTATTTGAG GAACTCCGCC GCCTGGCGCC CATTACCAGC
    GACCCCACAG AAGCCACCGC CGTGGGTGCC GTGGAGGCCT CCTTCAAGTG CTGCAGTGGG
    GCCATAATCG TCCTCACCAA GTCTGGCAGG TCTGCTCACC AGGTGGCCAG ATACCGCCCA
    CGTGCCCCCA TCATTGCTGT GACCCGGAAT CCCCAGACAG CTCGTCAGGC CCACCTGTAC
    CGTGGCATCT TCCCTGTGCT GTGCAAGGAC CCAGTCCAGG AGGCCTGGGC TGAGGACGTG
    GACCTCCGGG TGAACTTTGC CATGAATGTT GGCAAGGCCC GAGGCTTCTT CAAGAAGGGA
    GATGTGGTCA TTGTGCTGAC CGGATGGCGC CCTGGCTCCG GCTTCACCAA CACCATGCGT
    GTTGTTCCTG TGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48375
    Clone ID Related Accession (Same CDS sequence) XM_005254443.1
    Accession Version XM_005254443.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGACTCAAC CTTTCAATTA TTCTGCTTTT GAGAGAATTA TCTTCGCTGG CTCTCCAGGG 
    CACACCGTAT TCAGCTCTGA GCGGTCTTTG CTAGTGAGGC CAAGGAGCCA CCCTGAGCCA
    AAAGGGGAGC ATTATGTCAC CGGAAGCCCA ACCCCAGAGA ACCAAAGGAC CTCAGCAGCC
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATTGCCCGTG AGGCAGAGGC TGCCATCTAC CACTTGCAAT TATTTGAGGA ACTCCGCCGC
    CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCCACCGCCG TGGGTGCCGT GGAGGCCTCC
    TTCAAGTGCT GCAGTGGGGC CATAATCGTC CTCACCAAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005254500.1
    CDS67..1842
    Translation

    Target ORF information:

    RefSeq Version XM_005254443.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005254443.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGACTCAAC CTTTCAATTA TTCTGCTTTT GAGAGAATTA TCTTCGCTGG CTCTCCAGGG 
    CACACCGTAT TCAGCTCTGA GCGGTCTTTG CTAGTGAGGC CAAGGAGCCA CCCTGAGCCA
    AAAGGGGAGC ATTATGTCAC CGGAAGCCCA ACCCCAGAGA ACCAAAGGAC CTCAGCAGCC
    ATGTCGAAGC CCCATAGTGA AGCCGGGACT GCCTTCATTC AGACCCAGCA GCTGCACGCA
    GCCATGGCTG ACACATTCCT GGAGCACATG TGCCGCCTGG ACATTGATTC ACCACCCATC
    ACAGCCCGGA ACACTGGCAT CATCTGTACC ATTGGCCCAG CTTCCCGATC AGTGGAGACG
    TTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGTC TGAACTTCTC TCATGGAACT
    CATGAGTACC ATGCGGAGAC CATCAAGAAT GTGCGCACAG CCACGGAAAG CTTTGCTTCT
    GACCCCATCC TCTACCGGCC CGTTGCTGTG GCTCTAGACA CTAAAGGACC TGAGATCCGA
    ACTGGGCTCA TCAAGGGCAG CGGCACTGCA GAGGTGGAGC TGAAGAAGGG AGCCACTCTC
    AAAATCACGC TGGATAACGC CTACATGGAA AAGTGTGACG AGAACATCCT GTGGCTGGAC
    TACAAGAACA TCTGCAAGGT GGTGGAAGTG GGCAGCAAGA TCTACGTGGA TGATGGGCTT
    ATTTCTCTCC AGGTGAAGCA GAAAGGTGCC GACTTCCTGG TGACGGAGGT GGAAAATGGT
    GGCTCCTTGG GCAGCAAGAA GGGTGTGAAC CTTCCTGGGG CTGCTGTGGA CTTGCCTGCT
    GTGTCGGAGA AGGACATCCA GGATCTGAAG TTTGGGGTCG AGCAGGATGT TGATATGGTG
    TTTGCGTCAT TCATCCGCAA GGCATCTGAT GTCCATGAAG TTAGGAAGGT CCTGGGAGAG
    AAGGGAAAGA ACATCAAGAT TATCAGCAAA ATCGAGAATC ATGAGGGGGT TCGGAGGTTT
    GATGAAATCC TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGATCT AGGCATTGAG
    ATTCCTGCAG AGAAGGTCTT CCTTGCTCAG AAGATGATGA TTGGACGGTG CAACCGAGCT
    GGGAAGCCTG TCATCTGTGC TACTCAGATG CTGGAGAGCA TGATCAAGAA GCCCCGCCCC
    ACTCGGGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCCGA CTGCATCATG
    CTGTCTGGAG AAACAGCCAA AGGGGACTAT CCTCTGGAGG CTGTGCGCAT GCAGCACCTG
    ATTGCCCGTG AGGCAGAGGC TGCCATCTAC CACTTGCAAT TATTTGAGGA ACTCCGCCGC
    CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCCACCGCCG TGGGTGCCGT GGAGGCCTCC
    TTCAAGTGCT GCAGTGGGGC CATAATCGTC CTCACCAAGT CTGGCAGGTC TGCTCACCAG
    GTGGCCAGAT ACCGCCCACG TGCCCCCATC ATTGCTGTGA CCCGGAATCC CCAGACAGCT
    CGTCAGGCCC ACCTGTACCG TGGCATCTTC CCTGTGCTGT GCAAGGACCC AGTCCAGGAG
    GCCTGGGCTG AGGACGTGGA CCTCCGGGTG AACTTTGCCA TGAATGTTGG CAAGGCCCGA
    GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GATGGCGCCC TGGCTCCGGC
    TTCACCAACA CCATGCGTGT TGTTCCTGTG CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59742
    Clone ID Related Accession (Same CDS sequence) XM_011521670.2
    Accession Version XM_011521670.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pyruvate kinase PKM isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521670.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCAGTGGA GCTCAGAGAG AGGAGAACGG CTCCTCACGC CTGGGGCCTG CTCTTCAGAA 
    GTCCCCAGCG CCGTTCCTTC CAGATCAGGC GGGACCTCAG CAGCCATGTC GAAGCCCCAT
    AGTGAAGCCG GGACTGCCTT CATTCAGACC CAGCAGCTGC ACGCAGCCAT GGCTGACACA
    TTCCTGGAGC ACATGTGCCG CCTGGACATT GATTCACCAC CCATCACAGC CCGGAACACT
    GGCATCATCT GTACCATTGG CCCAGCTTCC CGATCAGTGG AGACGTTGAA GGAGATGATT
    AAGTCTGGAA TGAATGTGGC TCGTCTGAAC TTCTCTCATG GAACTCATGA GTACCATGCG
    GAGACCATCA AGAATGTGCG CACAGCCACG GAAAGCTTTG CTTCTGACCC CATCCTCTAC
    CGGCCCGTTG CTGTGGCTCT AGACACTAAA GGACCTGAGA TCCGAACTGG GCTCATCAAG
    GGCAGCGGCA CTGCAGAGGT GGAGCTGAAG AAGGGAGCCA CTCTCAAAAT CACGCTGGAT
    AACGCCTACA TGGAAAAGTG TGACGAGAAC ATCCTGTGGC TGGACTACAA GAACATCTGC
    AAGGTGGTGG AAGTGGGCAG CAAGATCTAC GTGGATGATG GGCTTATTTC TCTCCAGGTG
    AAGCAGAAAG GTGCCGACTT CCTGGTGACG GAGGTGGAAA ATGGTGGCTC CTTGGGCAGC
    AAGAAGGGTG TGAACCTTCC TGGGGCTGCT GTGGACTTGC CTGCTGTGTC GGAGAAGGAC
    ATCCAGGATC TGAAGTTTGG GGTCGAGCAG GATGTTGATA TGGTGTTTGC GTCATTCATC
    CGCAAGGCAT CTGATGTCCA TGAAGTTAGG AAGGTCCTGG GAGAGAAGGG AAAGAACATC
    AAGATTATCA GCAAAATCGA GAATCATGAG GGGGTTCGGA GGTTTGATGA AATCCTGGAG
    GCCAGTGATG GGATCATGGT GGCTCGTGGT GATCTAGGCA TTGAGATTCC TGCAGAGAAG
    GTCTTCCTTG CTCAGAAGAT GATGATTGGA CGGTGCAACC GAGCTGGGAA GCCTGTCATC
    TGTGCTACTC AGATGCTGGA GAGCATGATC AAGAAGCCCC GCCCCACTCG GGCTGAAGGC
    AGTGATGTGG CCAATGCAGT CCTGGATGGA GCCGACTGCA TCATGCTGTC TGGAGAAACA
    GCCAAAGGGG ACTATCCTCT GGAGGCTGTG CGCATGCAGC ACCTGATTGC CCGTGAGGCA
    GAGGCTGCCA TCTACCACTT GCAATTATTT GAGGAACTCC GCCGCCTGGC GCCCATTACC
    AGCGACCCCA CAGAAGCCAC CGCCGTGGGT GCCGTGGAGG CCTCCTTCAA GTGCTGCAGT
    GGGGCCATAA TCGTCCTCAC CAAGTCTGGC AGGTCTGCTC ACCAGGTGGC CAGATACCGC
    CCACGTGCCC CCATCATTGC TGTGACCCGG AATCCCCAGA CAGCTCGTCA GGCCCACCTG
    TACCGTGGCA TCTTCCCTGT GCTGTGCAAG GACCCAGTCC AGGAGGCCTG GGCTGAGGAC
    GTGGACCTCC GGGTGAACTT TGCCATGAAT GTTGGCAAGG CCCGAGGCTT CTTCAAGAAG
    GGAGATGTGG TCATTGTGCT GACCGGATGG CGCCCTGGCT CCGGCTTCAC CAACACCATG
    CGTGTTGTTC CTGTGCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519972.1
    CDS204..1904
    Translation

    Target ORF information:

    RefSeq Version XM_011521670.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate kinase M1/2 (PKM), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521670.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCAGTGGA GCTCAGAGAG AGGAGAACGG CTCCTCACGC CTGGGGCCTG CTCTTCAGAA 
    GTCCCCAGCG CCGTTCCTTC CAGATCAGGC GGGACCTCAG CAGCCATGTC GAAGCCCCAT
    AGTGAAGCCG GGACTGCCTT CATTCAGACC CAGCAGCTGC ACGCAGCCAT GGCTGACACA
    TTCCTGGAGC ACATGTGCCG CCTGGACATT GATTCACCAC CCATCACAGC CCGGAACACT
    GGCATCATCT GTACCATTGG CCCAGCTTCC CGATCAGTGG AGACGTTGAA GGAGATGATT
    AAGTCTGGAA TGAATGTGGC TCGTCTGAAC TTCTCTCATG GAACTCATGA GTACCATGCG
    GAGACCATCA AGAATGTGCG CACAGCCACG GAAAGCTTTG CTTCTGACCC CATCCTCTAC
    CGGCCCGTTG CTGTGGCTCT AGACACTAAA GGACCTGAGA TCCGAACTGG GCTCATCAAG
    GGCAGCGGCA CTGCAGAGGT GGAGCTGAAG AAGGGAGCCA CTCTCAAAAT CACGCTGGAT
    AACGCCTACA TGGAAAAGTG TGACGAGAAC ATCCTGTGGC TGGACTACAA GAACATCTGC
    AAGGTGGTGG AAGTGGGCAG CAAGATCTAC GTGGATGATG GGCTTATTTC TCTCCAGGTG
    AAGCAGAAAG GTGCCGACTT CCTGGTGACG GAGGTGGAAA ATGGTGGCTC CTTGGGCAGC
    AAGAAGGGTG TGAACCTTCC TGGGGCTGCT GTGGACTTGC CTGCTGTGTC GGAGAAGGAC
    ATCCAGGATC TGAAGTTTGG GGTCGAGCAG GATGTTGATA TGGTGTTTGC GTCATTCATC
    CGCAAGGCAT CTGATGTCCA TGAAGTTAGG AAGGTCCTGG GAGAGAAGGG AAAGAACATC
    AAGATTATCA GCAAAATCGA GAATCATGAG GGGGTTCGGA GGTTTGATGA AATCCTGGAG
    GCCAGTGATG GGATCATGGT GGCTCGTGGT GATCTAGGCA TTGAGATTCC TGCAGAGAAG
    GTCTTCCTTG CTCAGAAGAT GATGATTGGA CGGTGCAACC GAGCTGGGAA GCCTGTCATC
    TGTGCTACTC AGATGCTGGA GAGCATGATC AAGAAGCCCC GCCCCACTCG GGCTGAAGGC
    AGTGATGTGG CCAATGCAGT CCTGGATGGA GCCGACTGCA TCATGCTGTC TGGAGAAACA
    GCCAAAGGGG ACTATCCTCT GGAGGCTGTG CGCATGCAGC ACCTGATTGC CCGTGAGGCA
    GAGGCTGCCA TCTACCACTT GCAATTATTT GAGGAACTCC GCCGCCTGGC GCCCATTACC
    AGCGACCCCA CAGAAGCCAC CGCCGTGGGT GCCGTGGAGG CCTCCTTCAA GTGCTGCAGT
    GGGGCCATAA TCGTCCTCAC CAAGTCTGGC AGGTCTGCTC ACCAGGTGGC CAGATACCGC
    CCACGTGCCC CCATCATTGC TGTGACCCGG AATCCCCAGA CAGCTCGTCA GGCCCACCTG
    TACCGTGGCA TCTTCCCTGT GCTGTGCAAG GACCCAGTCC AGGAGGCCTG GGCTGAGGAC
    GTGGACCTCC GGGTGAACTT TGCCATGAAT GTTGGCAAGG CCCGAGGCTT CTTCAAGAAG
    GGAGATGTGG TCATTGTGCT GACCGGATGG CGCCCTGGCT CCGGCTTCAC CAACACCATG
    CGTGTTGTTC CTGTGCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.