Pkm cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pkm
Entrez Gene ID	18746
Full Name	pyruvate kinase, muscle
Synonyms	AA414905,AL024370,AL024424,Pk-2,Pk-3,Pk3,Pkm2
General protein information	Preferred Names pyruvate kinase, muscle Names pyruvate kinase PKM pyruvate kinase 3 pyruvate kinase isozymes M1/M2 pyruvate kinase muscle isozyme
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 B\|9 32.03 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011242674.1	XP_011240976.1	pyruvate kinase PKM isoform X2
XM_011242673.2	XP_011240975.2	pyruvate kinase PKM isoform X2
XM_011242673.1	XP_011240975.1	pyruvate kinase PKM isoform X1
XM_011242674.2	XP_011240976.2	pyruvate kinase PKM isoform X1
XM_006510855.1	XP_006510918.1	pyruvate kinase PKM isoform X3
XM_006510856.2	XP_006510919.1	pyruvate kinase PKM isoform X4
NM_011099.3	NP_035229.2	pyruvate kinase PKM isoform M2
NM_001253883.1	NP_001240812.1	pyruvate kinase PKM isoform M1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PKM	NP_001193725.1
Gallus gallus (chicken)	PKM2	NP_990800.1
Drosophila melanogaster (fruit fly)	PyK	NP_524448.3
Schizosaccharomyces pombe (fission yeast)	pyk1	NP_594346.1
Arabidopsis thaliana (thale cress)	AT5G63680	NP_201173.1
Arabidopsis thaliana (thale cress)	AT5G08570	NP_196474.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004596	XP_315228.5
Kluyveromyces lactis	KLLA0F23397g	XP_456122.1
	PKM	XP_001099473.2
Rattus norvegicus (Norway rat)	Pkm	NP_445749.1
Magnaporthe oryzae (rice blast fungus)	MGG_08063	XP_003715015.1
Pan troglodytes (chimpanzee)	PKM	XP_003314790.1
Canis lupus familiaris (dog)	PKM	XP_005638614.1
Caenorhabditis elegans (roundworm)	pyk-1	NP_001251014.1
Caenorhabditis elegans (roundworm)	pyk-2	NP_001255515.1
Saccharomyces cerevisiae (baker's yeast)	CDC19	NP_009362.1
Neurospora crassa	NCU06075	XP_959838.1
Oryza sativa (rice)	Os04g0677500	NP_001054265.1
Bos taurus (cattle)	PKM	NP_001192656.1
Mus musculus (house mouse)	Pkm	NP_035229.2
Danio rerio (zebrafish)	pkma	NP_955365.1
Eremothecium gossypii	AGOS_ADR368W	NP_984464.1
Xenopus tropicalis (tropical clawed frog)	pkm	NP_001072532.1

Gene Ontology

The following Pkm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pkm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05678	NM_001253883.1 Latest version!	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu05678	XM_011242673.1 Latest version!	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu42581	XM_006510856.2 Latest version!	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu68439	XM_011242674.1 Latest version!	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu15721	NM_011099.3 Latest version!	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu15721	XM_006510855.1 Latest version!	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu90104	XM_011242674.2 Latest version!	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu90105	XM_011242673.2 Latest version!	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pkm ( NM_001253883.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001240812.1 Mus musculus pyruvate kinase, muscle (Pkm), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05678
Clone ID Related Accession (Same CDS sequence)	XM_011242673.1 , NM_001253883.1
Accession Version	NM_001253883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	pyruvate kinase PKM isoform M1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB846337.1, AK155655.1, BI985789.1, AK135397.1 and BB819292.1. Transcript Variant: This variant (1) encodes isoform M1. Isoforms M1 and M2 are identical in length but have different sequences. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCCGAAGC CACACAGTGA AGCAGGGACT GCCTTCATTC AGACCCAGCA GCTCCATGCA 
GCCATGGCTG ACACCTTCCT GGAACACATG TGCCGCCTGG ACATTGACTC TGCCCCCATC 
ACGGCCCGCA ACACTGGCAT CATTTGTACC ATTGGGCCTG CTTCCCGATC TGTGGAGATG 
CTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGGC TGAATTTCTC TCATGGAACC 
CATGAGTACC ATGCAGAGAC CATCAAGAAT GTCCGTGAAG CCACAGAAAG CTTTGCATCT 
GATCCCATTC TCTACCGTCC TGTTGCGGTG GCTCTGGATA CAAAGGGACC TGAGATCCGG 
ACTGGACTCA TCAAGGGCAG CGGCACCGCT GAGGTGGAGC TGAAGAAGGG AGCCACTCTG 
AAGATCACCC TGGACAACGC TTACATGGAG AAGTGTGACG AGAACATCCT GTGGCTGGAC 
TACAAGAACA TCTGCAAGGT GGTGGAGGTG GGCAGCAAGA TCTACGTGGA CGATGGGCTC 
ATCTCACTGC AGGTGAAGGA GAAAGGCGCT GACTTCCTGG TGACGGAGGT GGAGAATGGT 
GGCTCCTTGG GCAGCAAGAA GGGCGTGAAC CTGCCGGGCG CTGCTGTGGA TCTCCCCGCT 
GTGTCGGAAA AGGACATCCA GGACCTGAAG TTTGGGGTGG AGCAGGATGT GGACATGGTG 
TTTGCATCTT TCATCCGCAA GGCAGCCGAC GTGCATGAAG TCAGGAAGGT GCTGGGAGAG 
AAGGGCAAGA ACATCAAGAT CATCAGCAAA ATCGAGAACC ATGAAGGCGT CCGCAGGTTT 
GATGAGATCT TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGACCT GGGCATTGAG 
ATTCCTGCAG AGAAGGTCTT CCTGGCTCAG AAGATGATGA TCGGGCGATG CAACCGAGCT 
GGGAAGCCTG TCATCTGTGC CACACAGATG CTGGAGAGCA TGATCAAGAA GCCACGCCCC 
ACCCGTGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCAGA CTGCATCATG 
CTGTCTGGAG AAACAGCCAA GGGGGACTAC CCTCTGGAGG CTGTTCGCAT GCAGCACCTG 
ATAGCTCGGG AGGCTGAGGC AGCCATGTTC CACCGTCTGC TGTTTGAAGA GCTTGTGCGA 
GCCTCCAGTC ACTCCACAGA CCTCATGGAG GCCATGGCCA TGGGCAGCGT GGAGGCCTCT 
TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTCACGGAGT CTGGCAGGAG TGCTCACCAA 
GTGGCCAGGT ACCGCCCTCG GGCTCCTATC ATTGCCGTGA CTCGAAATCC CCAGACTGCT 
CGCCAGGCCC ATCTGTACCG TGGCATCTTC CCTGTGCTGT GTAAGGATGC CGTGCTGAAT 
GCCTGGGCTG AGGATGTCGA CCTTCGTGTA AACTTGGCCA TGGATGTTGG CAAGGCCCGA 
GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GGTGGCGCCC TGGCTCTGGA 
TTCACCAACA CCATGCGTGT AGTGCCTGTA CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001240812.1
CDS	296..1891
Translation	MPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRLLFEELVRASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

Target ORF information:

RefSeq Version	NM_001253883.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001253883.1

ORF Insert Sequence:

ATGCCGAAGC CACACAGTGA AGCAGGGACT GCCTTCATTC AGACCCAGCA GCTCCATGCA 
GCCATGGCTG ACACCTTCCT GGAACACATG TGCCGCCTGG ACATTGACTC TGCCCCCATC 
ACGGCCCGCA ACACTGGCAT CATTTGTACC ATTGGGCCTG CTTCCCGATC TGTGGAGATG 
CTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGGC TGAATTTCTC TCATGGAACC 
CATGAGTACC ATGCAGAGAC CATCAAGAAT GTCCGTGAAG CCACAGAAAG CTTTGCATCT 
GATCCCATTC TCTACCGTCC TGTTGCGGTG GCTCTGGATA CAAAGGGACC TGAGATCCGG 
ACTGGACTCA TCAAGGGCAG CGGCACCGCT GAGGTGGAGC TGAAGAAGGG AGCCACTCTG 
AAGATCACCC TGGACAACGC TTACATGGAG AAGTGTGACG AGAACATCCT GTGGCTGGAC 
TACAAGAACA TCTGCAAGGT GGTGGAGGTG GGCAGCAAGA TCTACGTGGA CGATGGGCTC 
ATCTCACTGC AGGTGAAGGA GAAAGGCGCT GACTTCCTGG TGACGGAGGT GGAGAATGGT 
GGCTCCTTGG GCAGCAAGAA GGGCGTGAAC CTGCCGGGCG CTGCTGTGGA TCTCCCCGCT 
GTGTCGGAAA AGGACATCCA GGACCTGAAG TTTGGGGTGG AGCAGGATGT GGACATGGTG 
TTTGCATCTT TCATCCGCAA GGCAGCCGAC GTGCATGAAG TCAGGAAGGT GCTGGGAGAG 
AAGGGCAAGA ACATCAAGAT CATCAGCAAA ATCGAGAACC ATGAAGGCGT CCGCAGGTTT 
GATGAGATCT TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGACCT GGGCATTGAG 
ATTCCTGCAG AGAAGGTCTT CCTGGCTCAG AAGATGATGA TCGGGCGATG CAACCGAGCT 
GGGAAGCCTG TCATCTGTGC CACACAGATG CTGGAGAGCA TGATCAAGAA GCCACGCCCC 
ACCCGTGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCAGA CTGCATCATG 
CTGTCTGGAG AAACAGCCAA GGGGGACTAC CCTCTGGAGG CTGTTCGCAT GCAGCACCTG 
ATAGCTCGGG AGGCTGAGGC AGCCATGTTC CACCGTCTGC TGTTTGAAGA GCTTGTGCGA 
GCCTCCAGTC ACTCCACAGA CCTCATGGAG GCCATGGCCA TGGGCAGCGT GGAGGCCTCT 
TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTCACGGAGT CTGGCAGGAG TGCTCACCAA 
GTGGCCAGGT ACCGCCCTCG GGCTCCTATC ATTGCCGTGA CTCGAAATCC CCAGACTGCT 
CGCCAGGCCC ATCTGTACCG TGGCATCTTC CCTGTGCTGT GTAAGGATGC CGTGCTGAAT 
GCCTGGGCTG AGGATGTCGA CCTTCGTGTA AACTTGGCCA TGGATGTTGG CAAGGCCCGA 
GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GGTGGCGCCC TGGCTCTGGA 
TTCACCAACA CCATGCGTGT AGTGCCTGTA CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkm ( XM_011242673.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240975.1 Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu05678
Clone ID Related Accession (Same CDS sequence)	XM_011242673.1 , NM_001253883.1
Accession Version	XM_011242673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	pyruvate kinase PKM isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CACACAGTGA AGCAGGGACT GCCTTCATTC AGACCCAGCA GCTCCATGCA 
GCCATGGCTG ACACCTTCCT GGAACACATG TGCCGCCTGG ACATTGACTC TGCCCCCATC 
ACGGCCCGCA ACACTGGCAT CATTTGTACC ATTGGGCCTG CTTCCCGATC TGTGGAGATG 
CTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGGC TGAATTTCTC TCATGGAACC 
CATGAGTACC ATGCAGAGAC CATCAAGAAT GTCCGTGAAG CCACAGAAAG CTTTGCATCT 
GATCCCATTC TCTACCGTCC TGTTGCGGTG GCTCTGGATA CAAAGGGACC TGAGATCCGG 
ACTGGACTCA TCAAGGGCAG CGGCACCGCT GAGGTGGAGC TGAAGAAGGG AGCCACTCTG 
AAGATCACCC TGGACAACGC TTACATGGAG AAGTGTGACG AGAACATCCT GTGGCTGGAC 
TACAAGAACA TCTGCAAGGT GGTGGAGGTG GGCAGCAAGA TCTACGTGGA CGATGGGCTC 
ATCTCACTGC AGGTGAAGGA GAAAGGCGCT GACTTCCTGG TGACGGAGGT GGAGAATGGT 
GGCTCCTTGG GCAGCAAGAA GGGCGTGAAC CTGCCGGGCG CTGCTGTGGA TCTCCCCGCT 
GTGTCGGAAA AGGACATCCA GGACCTGAAG TTTGGGGTGG AGCAGGATGT GGACATGGTG 
TTTGCATCTT TCATCCGCAA GGCAGCCGAC GTGCATGAAG TCAGGAAGGT GCTGGGAGAG 
AAGGGCAAGA ACATCAAGAT CATCAGCAAA ATCGAGAACC ATGAAGGCGT CCGCAGGTTT 
GATGAGATCT TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGACCT GGGCATTGAG 
ATTCCTGCAG AGAAGGTCTT CCTGGCTCAG AAGATGATGA TCGGGCGATG CAACCGAGCT 
GGGAAGCCTG TCATCTGTGC CACACAGATG CTGGAGAGCA TGATCAAGAA GCCACGCCCC 
ACCCGTGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCAGA CTGCATCATG 
CTGTCTGGAG AAACAGCCAA GGGGGACTAC CCTCTGGAGG CTGTTCGCAT GCAGCACCTG 
ATAGCTCGGG AGGCTGAGGC AGCCATGTTC CACCGTCTGC TGTTTGAAGA GCTTGTGCGA 
GCCTCCAGTC ACTCCACAGA CCTCATGGAG GCCATGGCCA TGGGCAGCGT GGAGGCCTCT 
TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTCACGGAGT CTGGCAGGAG TGCTCACCAA 
GTGGCCAGGT ACCGCCCTCG GGCTCCTATC ATTGCCGTGA CTCGAAATCC CCAGACTGCT 
CGCCAGGCCC ATCTGTACCG TGGCATCTTC CCTGTGCTGT GTAAGGATGC CGTGCTGAAT 
GCCTGGGCTG AGGATGTCGA CCTTCGTGTA AACTTGGCCA TGGATGTTGG CAAGGCCCGA 
GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GGTGGCGCCC TGGCTCTGGA 
TTCACCAACA CCATGCGTGT AGTGCCTGTA CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240975.1
CDS	126..1721
Translation	MPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRLLFEELVRASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

Target ORF information:

RefSeq Version	XM_011242673.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242673.1

ORF Insert Sequence:

ATGCCGAAGC CACACAGTGA AGCAGGGACT GCCTTCATTC AGACCCAGCA GCTCCATGCA 
GCCATGGCTG ACACCTTCCT GGAACACATG TGCCGCCTGG ACATTGACTC TGCCCCCATC 
ACGGCCCGCA ACACTGGCAT CATTTGTACC ATTGGGCCTG CTTCCCGATC TGTGGAGATG 
CTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGGC TGAATTTCTC TCATGGAACC 
CATGAGTACC ATGCAGAGAC CATCAAGAAT GTCCGTGAAG CCACAGAAAG CTTTGCATCT 
GATCCCATTC TCTACCGTCC TGTTGCGGTG GCTCTGGATA CAAAGGGACC TGAGATCCGG 
ACTGGACTCA TCAAGGGCAG CGGCACCGCT GAGGTGGAGC TGAAGAAGGG AGCCACTCTG 
AAGATCACCC TGGACAACGC TTACATGGAG AAGTGTGACG AGAACATCCT GTGGCTGGAC 
TACAAGAACA TCTGCAAGGT GGTGGAGGTG GGCAGCAAGA TCTACGTGGA CGATGGGCTC 
ATCTCACTGC AGGTGAAGGA GAAAGGCGCT GACTTCCTGG TGACGGAGGT GGAGAATGGT 
GGCTCCTTGG GCAGCAAGAA GGGCGTGAAC CTGCCGGGCG CTGCTGTGGA TCTCCCCGCT 
GTGTCGGAAA AGGACATCCA GGACCTGAAG TTTGGGGTGG AGCAGGATGT GGACATGGTG 
TTTGCATCTT TCATCCGCAA GGCAGCCGAC GTGCATGAAG TCAGGAAGGT GCTGGGAGAG 
AAGGGCAAGA ACATCAAGAT CATCAGCAAA ATCGAGAACC ATGAAGGCGT CCGCAGGTTT 
GATGAGATCT TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGACCT GGGCATTGAG 
ATTCCTGCAG AGAAGGTCTT CCTGGCTCAG AAGATGATGA TCGGGCGATG CAACCGAGCT 
GGGAAGCCTG TCATCTGTGC CACACAGATG CTGGAGAGCA TGATCAAGAA GCCACGCCCC 
ACCCGTGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCAGA CTGCATCATG 
CTGTCTGGAG AAACAGCCAA GGGGGACTAC CCTCTGGAGG CTGTTCGCAT GCAGCACCTG 
ATAGCTCGGG AGGCTGAGGC AGCCATGTTC CACCGTCTGC TGTTTGAAGA GCTTGTGCGA 
GCCTCCAGTC ACTCCACAGA CCTCATGGAG GCCATGGCCA TGGGCAGCGT GGAGGCCTCT 
TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTCACGGAGT CTGGCAGGAG TGCTCACCAA 
GTGGCCAGGT ACCGCCCTCG GGCTCCTATC ATTGCCGTGA CTCGAAATCC CCAGACTGCT 
CGCCAGGCCC ATCTGTACCG TGGCATCTTC CCTGTGCTGT GTAAGGATGC CGTGCTGAAT 
GCCTGGGCTG AGGATGTCGA CCTTCGTGTA AACTTGGCCA TGGATGTTGG CAAGGCCCGA 
GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GGTGGCGCCC TGGCTCTGGA 
TTCACCAACA CCATGCGTGT AGTGCCTGTA CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkm ( XM_006510856.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510919.1 Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu42581
Clone ID Related Accession (Same CDS sequence)	XM_006510856.2
Accession Version	XM_006510856.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	pyruvate kinase PKM isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006510856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATCAG AAGCCCAGAC CCAGAGAACC AAAGGACTTC AGGAACCATG CCGAAGCCAC 
ACAGTGAAGC AGGGACTGCC TTCATTCAGA CCCAGCAGCT CCATGCAGCC ATGGCTGACA 
CCTTCCTGGA ACACATGTGC CGCCTGGACA TTGACTCTGC CCCCATCACG GCCCGCAACA 
CTGGCATCAT TTGTACCATT GTACCATGCA GAGACCATCA AGAATGTCCG TGAAGCCACA 
GAAAGCTTTG CATCTGATCC CATTCTCTAC CGTCCTGTTG CGGTGGCTCT GGATACAAAG 
GGACCTGAGA TCCGGACTGG ACTCATCAAG GGCAGCGGCA CCGCTGAGGT GGAGCTGAAG 
AAGGGAGCCA CTCTGAAGAT CACCCTGGAC AACGCTTACA TGGAGAAGTG TGACGAGAAC 
ATCCTGTGGC TGGACTACAA GAACATCTGC AAGGTGGTGG AGGTGGGCAG CAAGATCTAC 
GTGGACGATG GGCTCATCTC ACTGCAGGTG AAGGAGAAAG GCGCTGACTT CCTGGTGACG 
GAGGTGGAGA ATGGTGGCTC CTTGGGCAGC AAGAAGGGCG TGAACCTGCC GGGCGCTGCT 
GTGGATCTCC CCGCTGTGTC GGAAAAGGAC ATCCAGGACC TGAAGTTTGG GGTGGAGCAG 
GATGTGGACA TGGTGTTTGC ATCTTTCATC CGCAAGGCAG CCGACGTGCA TGAAGTCAGG 
AAGGTGCTGG GAGAGAAGGG CAAGAACATC AAGATCATCA GCAAAATCGA GAACCATGAA 
GGCGTCCGCA GGTTTGATGA GATCTTGGAG GCCAGTGATG GGATCATGGT GGCTCGTGGT 
GACCTGGGCA TTGAGATTCC TGCAGAGAAG GTCTTCCTGG CTCAGAAGAT GATGATCGGG 
CGATGCAACC GAGCTGGGAA GCCTGTCATC TGTGCCACAC AGATGCTGGA GAGCATGATC 
AAGAAGCCAC GCCCCACCCG TGCTGAAGGC AGTGATGTGG CCAATGCAGT CCTGGATGGA 
GCAGACTGCA TCATGCTGTC TGGAGAAACA GCCAAGGGGG ACTACCCTCT GGAGGCTGTT 
CGCATGCAGC ACCTGATTGC CCGAGAGGCA GAGGCTGCCA TCTACCACTT GCAGCTATTC 
GAGGAACTCC GCCGCCTGGC GCCCATTACC AGCGACCCCA CAGAAGCTGC CGCCGTGGGT 
GCCGTGGAGG CCTCCTTCAA GTGCTGCAGT GGGGCCATTA TCGTGCTCAC CAAGTCTGGC 
AGGAGTGCTC ACCAAGTGGC CAGGTACCGC CCTCGGGCTC CTATCATTGC CGTGACTCGA 
AATCCCCAGA CTGCTCGCCA GGCCCATCTG TACCGTGGCA TCTTCCCTGT GCTGTGTAAG 
GATGCCGTGC TGAATGCCTG GGCTGAGGAT GTCGACCTTC GTGTAAACTT GGCCATGGAT 
GTTGGCAAGG CCCGAGGCTT CTTCAAGAAG GGAGATGTGG TCATTGTGCT GACCGGGTGG 
CGCCCTGGCT CTGGATTCAC CAACACCATG CGTGTAGTGC CTGTACCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510919.1
CDS	16..1566
Translation	MPSEAQTQRTKGLQEPCRSHTVKQGLPSFRPSSSMQPWLTPSWNTCAAWTLTLPPSRPATLASFVPLYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

Target ORF information:

RefSeq Version	XM_006510856.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510856.2

ORF Insert Sequence:

ATGCCATCAG AAGCCCAGAC CCAGAGAACC AAAGGACTTC AGGAACCATG CCGAAGCCAC 
ACAGTGAAGC AGGGACTGCC TTCATTCAGA CCCAGCAGCT CCATGCAGCC ATGGCTGACA 
CCTTCCTGGA ACACATGTGC CGCCTGGACA TTGACTCTGC CCCCATCACG GCCCGCAACA 
CTGGCATCAT TTGTACCATT GTACCATGCA GAGACCATCA AGAATGTCCG TGAAGCCACA 
GAAAGCTTTG CATCTGATCC CATTCTCTAC CGTCCTGTTG CGGTGGCTCT GGATACAAAG 
GGACCTGAGA TCCGGACTGG ACTCATCAAG GGCAGCGGCA CCGCTGAGGT GGAGCTGAAG 
AAGGGAGCCA CTCTGAAGAT CACCCTGGAC AACGCTTACA TGGAGAAGTG TGACGAGAAC 
ATCCTGTGGC TGGACTACAA GAACATCTGC AAGGTGGTGG AGGTGGGCAG CAAGATCTAC 
GTGGACGATG GGCTCATCTC ACTGCAGGTG AAGGAGAAAG GCGCTGACTT CCTGGTGACG 
GAGGTGGAGA ATGGTGGCTC CTTGGGCAGC AAGAAGGGCG TGAACCTGCC GGGCGCTGCT 
GTGGATCTCC CCGCTGTGTC GGAAAAGGAC ATCCAGGACC TGAAGTTTGG GGTGGAGCAG 
GATGTGGACA TGGTGTTTGC ATCTTTCATC CGCAAGGCAG CCGACGTGCA TGAAGTCAGG 
AAGGTGCTGG GAGAGAAGGG CAAGAACATC AAGATCATCA GCAAAATCGA GAACCATGAA 
GGCGTCCGCA GGTTTGATGA GATCTTGGAG GCCAGTGATG GGATCATGGT GGCTCGTGGT 
GACCTGGGCA TTGAGATTCC TGCAGAGAAG GTCTTCCTGG CTCAGAAGAT GATGATCGGG 
CGATGCAACC GAGCTGGGAA GCCTGTCATC TGTGCCACAC AGATGCTGGA GAGCATGATC 
AAGAAGCCAC GCCCCACCCG TGCTGAAGGC AGTGATGTGG CCAATGCAGT CCTGGATGGA 
GCAGACTGCA TCATGCTGTC TGGAGAAACA GCCAAGGGGG ACTACCCTCT GGAGGCTGTT 
CGCATGCAGC ACCTGATTGC CCGAGAGGCA GAGGCTGCCA TCTACCACTT GCAGCTATTC 
GAGGAACTCC GCCGCCTGGC GCCCATTACC AGCGACCCCA CAGAAGCTGC CGCCGTGGGT 
GCCGTGGAGG CCTCCTTCAA GTGCTGCAGT GGGGCCATTA TCGTGCTCAC CAAGTCTGGC 
AGGAGTGCTC ACCAAGTGGC CAGGTACCGC CCTCGGGCTC CTATCATTGC CGTGACTCGA 
AATCCCCAGA CTGCTCGCCA GGCCCATCTG TACCGTGGCA TCTTCCCTGT GCTGTGTAAG 
GATGCCGTGC TGAATGCCTG GGCTGAGGAT GTCGACCTTC GTGTAAACTT GGCCATGGAT 
GTTGGCAAGG CCCGAGGCTT CTTCAAGAAG GGAGATGTGG TCATTGTGCT GACCGGGTGG 
CGCCCTGGCT CTGGATTCAC CAACACCATG CGTGTAGTGC CTGTACCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkm ( XM_011242674.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240976.1 Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X2, mRNA.

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CloneID	OMu68439
Clone ID Related Accession (Same CDS sequence)	XM_011242674.1
Accession Version	XM_011242674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	pyruvate kinase PKM isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCATT TGTCTTTCAG TTTCTCAATC ATCTCAAATA CCCACAGGCA AGTTAACTGC 
TCTTCAGGGC AAAGCATACT CCACTCTGAG CGATCTTTCC AAGTGAGGCC ACCAAGCCAC 
ACTGAGTCAA CAAAACAGCA ATATGCCATC AGAAGCCCAG ACCCAGAGAA CCAAAGGACT 
TCAGGAACCA TGCCGAAGCC ACACAGTGAA GCAGGGACTG CCTTCATTCA GACCCAGCAG 
CTCCATGCAG CCATGGCTGA CACCTTCCTG GAACACATGT GCCGCCTGGA CATTGACTCT 
GCCCCCATCA CGGCCCGCAA CACTGGCATC ATTTGTACCA TTGGGCCTGC TTCCCGATCT 
GTGGAGATGC TGAAGGAGAT GATTAAGTCT GGAATGAATG TGGCTCGGCT GAATTTCTCT 
CATGGAACCC ATGAGTACCA TGCAGAGACC ATCAAGAATG TCCGTGAAGC CACAGAAAGC 
TTTGCATCTG ATCCCATTCT CTACCGTCCT GTTGCGGTGG CTCTGGATAC AAAGGGACCT 
GAGATCCGGA CTGGACTCAT CAAGGGCAGC GGCACCGCTG AGGTGGAGCT GAAGAAGGGA 
GCCACTCTGA AGATCACCCT GGACAACGCT TACATGGAGA AGTGTGACGA GAACATCCTG 
TGGCTGGACT ACAAGAACAT CTGCAAGGTG GTGGAGGTGG GCAGCAAGAT CTACGTGGAC 
GATGGGCTCA TCTCACTGCA GGTGAAGGAG AAAGGCGCTG ACTTCCTGGT GACGGAGGTG 
GAGAATGGTG GCTCCTTGGG CAGCAAGAAG GGCGTGAACC TGCCGGGCGC TGCTGTGGAT 
CTCCCCGCTG TGTCGGAAAA GGACATCCAG GACCTGAAGT TTGGGGTGGA GCAGGATGTG 
GACATGGTGT TTGCATCTTT CATCCGCAAG GCAGCCGACG TGCATGAAGT CAGGAAGGTG 
CTGGGAGAGA AGGGCAAGAA CATCAAGATC ATCAGCAAAA TCGAGAACCA TGAAGGCGTC 
CGCAGGTTTG ATGAGATCTT GGAGGCCAGT GATGGGATCA TGGTGGCTCG TGGTGACCTG 
GGCATTGAGA TTCCTGCAGA GAAGGTCTTC CTGGCTCAGA AGATGATGAT CGGGCGATGC 
AACCGAGCTG GGAAGCCTGT CATCTGTGCC ACACAGATGC TGGAGAGCAT GATCAAGAAG 
CCACGCCCCA CCCGTGCTGA AGGCAGTGAT GTGGCCAATG CAGTCCTGGA TGGAGCAGAC 
TGCATCATGC TGTCTGGAGA AACAGCCAAG GGGGACTACC CTCTGGAGGC TGTTCGCATG 
CAGCACCTGA TTGCCCGAGA GGCAGAGGCT GCCATCTACC ACTTGCAGCT ATTCGAGGAA 
CTCCGCCGCC TGGCGCCCAT TACCAGCGAC CCCACAGAAG CTGCCGCCGT GGGTGCCGTG 
GAGGCCTCCT TCAAGTGCTG CAGTGGGGCC ATTATCGTGC TCACCAAGTC TGGCAGGAGT 
GCTCACCAAG TGGCCAGGTA CCGCCCTCGG GCTCCTATCA TTGCCGTGAC TCGAAATCCC 
CAGACTGCTC GCCAGGCCCA TCTGTACCGT GGCATCTTCC CTGTGCTGTG TAAGGATGCC 
GTGCTGAATG CCTGGGCTGA GGATGTCGAC CTTCGTGTAA ACTTGGCCAT GGATGTTGGC 
AAGGCCCGAG GCTTCTTCAA GAAGGGAGAT GTGGTCATTG TGCTGACCGG GTGGCGCCCT 
GGCTCTGGAT TCACCAACAC CATGCGTGTA GTGCCTGTAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240976.1
CDS	48..1832
Translation	MTHLSFSFSIISNTHRQVNCSSGQSILHSERSFQVRPPSHTESTKQQYAIRSPDPENQRTSGTMPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

Target ORF information:

RefSeq Version	XM_011242674.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242674.1

ORF Insert Sequence:

ATGACTCATT TGTCTTTCAG TTTCTCAATC ATCTCAAATA CCCACAGGCA AGTTAACTGC 
TCTTCAGGGC AAAGCATACT CCACTCTGAG CGATCTTTCC AAGTGAGGCC ACCAAGCCAC 
ACTGAGTCAA CAAAACAGCA ATATGCCATC AGAAGCCCAG ACCCAGAGAA CCAAAGGACT 
TCAGGAACCA TGCCGAAGCC ACACAGTGAA GCAGGGACTG CCTTCATTCA GACCCAGCAG 
CTCCATGCAG CCATGGCTGA CACCTTCCTG GAACACATGT GCCGCCTGGA CATTGACTCT 
GCCCCCATCA CGGCCCGCAA CACTGGCATC ATTTGTACCA TTGGGCCTGC TTCCCGATCT 
GTGGAGATGC TGAAGGAGAT GATTAAGTCT GGAATGAATG TGGCTCGGCT GAATTTCTCT 
CATGGAACCC ATGAGTACCA TGCAGAGACC ATCAAGAATG TCCGTGAAGC CACAGAAAGC 
TTTGCATCTG ATCCCATTCT CTACCGTCCT GTTGCGGTGG CTCTGGATAC AAAGGGACCT 
GAGATCCGGA CTGGACTCAT CAAGGGCAGC GGCACCGCTG AGGTGGAGCT GAAGAAGGGA 
GCCACTCTGA AGATCACCCT GGACAACGCT TACATGGAGA AGTGTGACGA GAACATCCTG 
TGGCTGGACT ACAAGAACAT CTGCAAGGTG GTGGAGGTGG GCAGCAAGAT CTACGTGGAC 
GATGGGCTCA TCTCACTGCA GGTGAAGGAG AAAGGCGCTG ACTTCCTGGT GACGGAGGTG 
GAGAATGGTG GCTCCTTGGG CAGCAAGAAG GGCGTGAACC TGCCGGGCGC TGCTGTGGAT 
CTCCCCGCTG TGTCGGAAAA GGACATCCAG GACCTGAAGT TTGGGGTGGA GCAGGATGTG 
GACATGGTGT TTGCATCTTT CATCCGCAAG GCAGCCGACG TGCATGAAGT CAGGAAGGTG 
CTGGGAGAGA AGGGCAAGAA CATCAAGATC ATCAGCAAAA TCGAGAACCA TGAAGGCGTC 
CGCAGGTTTG ATGAGATCTT GGAGGCCAGT GATGGGATCA TGGTGGCTCG TGGTGACCTG 
GGCATTGAGA TTCCTGCAGA GAAGGTCTTC CTGGCTCAGA AGATGATGAT CGGGCGATGC 
AACCGAGCTG GGAAGCCTGT CATCTGTGCC ACACAGATGC TGGAGAGCAT GATCAAGAAG 
CCACGCCCCA CCCGTGCTGA AGGCAGTGAT GTGGCCAATG CAGTCCTGGA TGGAGCAGAC 
TGCATCATGC TGTCTGGAGA AACAGCCAAG GGGGACTACC CTCTGGAGGC TGTTCGCATG 
CAGCACCTGA TTGCCCGAGA GGCAGAGGCT GCCATCTACC ACTTGCAGCT ATTCGAGGAA 
CTCCGCCGCC TGGCGCCCAT TACCAGCGAC CCCACAGAAG CTGCCGCCGT GGGTGCCGTG 
GAGGCCTCCT TCAAGTGCTG CAGTGGGGCC ATTATCGTGC TCACCAAGTC TGGCAGGAGT 
GCTCACCAAG TGGCCAGGTA CCGCCCTCGG GCTCCTATCA TTGCCGTGAC TCGAAATCCC 
CAGACTGCTC GCCAGGCCCA TCTGTACCGT GGCATCTTCC CTGTGCTGTG TAAGGATGCC 
GTGCTGAATG CCTGGGCTGA GGATGTCGAC CTTCGTGTAA ACTTGGCCAT GGATGTTGGC 
AAGGCCCGAG GCTTCTTCAA GAAGGGAGAT GTGGTCATTG TGCTGACCGG GTGGCGCCCT 
GGCTCTGGAT TCACCAACAC CATGCGTGTA GTGCCTGTAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkm ( NM_011099.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035229.2 Mus musculus pyruvate kinase, muscle (Pkm), transcript variant 2, mRNA.

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CloneID	OMu15721
Clone ID Related Accession (Same CDS sequence)	XM_006510855.1 , NM_011099.3
Accession Version	NM_011099.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	pyruvate kinase PKM isoform M2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB846337.1, AK135397.1 and BB819292.1. On Dec 9, 2011 this sequence version replaced NM_011099.2. Transcript Variant: This variant (2) lacks an in-frame exon and contains an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (M2) is identical in length to isoform M1, but has a different sequence. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016619.1, SRR1660815.6385.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCCGAAGC CACACAGTGA AGCAGGGACT GCCTTCATTC AGACCCAGCA GCTCCATGCA 
GCCATGGCTG ACACCTTCCT GGAACACATG TGCCGCCTGG ACATTGACTC TGCCCCCATC 
ACGGCCCGCA ACACTGGCAT CATTTGTACC ATTGGGCCTG CTTCCCGATC TGTGGAGATG 
CTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGGC TGAATTTCTC TCATGGAACC 
CATGAGTACC ATGCAGAGAC CATCAAGAAT GTCCGTGAAG CCACAGAAAG CTTTGCATCT 
GATCCCATTC TCTACCGTCC TGTTGCGGTG GCTCTGGATA CAAAGGGACC TGAGATCCGG 
ACTGGACTCA TCAAGGGCAG CGGCACCGCT GAGGTGGAGC TGAAGAAGGG AGCCACTCTG 
AAGATCACCC TGGACAACGC TTACATGGAG AAGTGTGACG AGAACATCCT GTGGCTGGAC 
TACAAGAACA TCTGCAAGGT GGTGGAGGTG GGCAGCAAGA TCTACGTGGA CGATGGGCTC 
ATCTCACTGC AGGTGAAGGA GAAAGGCGCT GACTTCCTGG TGACGGAGGT GGAGAATGGT 
GGCTCCTTGG GCAGCAAGAA GGGCGTGAAC CTGCCGGGCG CTGCTGTGGA TCTCCCCGCT 
GTGTCGGAAA AGGACATCCA GGACCTGAAG TTTGGGGTGG AGCAGGATGT GGACATGGTG 
TTTGCATCTT TCATCCGCAA GGCAGCCGAC GTGCATGAAG TCAGGAAGGT GCTGGGAGAG 
AAGGGCAAGA ACATCAAGAT CATCAGCAAA ATCGAGAACC ATGAAGGCGT CCGCAGGTTT 
GATGAGATCT TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGACCT GGGCATTGAG 
ATTCCTGCAG AGAAGGTCTT CCTGGCTCAG AAGATGATGA TCGGGCGATG CAACCGAGCT 
GGGAAGCCTG TCATCTGTGC CACACAGATG CTGGAGAGCA TGATCAAGAA GCCACGCCCC 
ACCCGTGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCAGA CTGCATCATG 
CTGTCTGGAG AAACAGCCAA GGGGGACTAC CCTCTGGAGG CTGTTCGCAT GCAGCACCTG 
ATTGCCCGAG AGGCAGAGGC TGCCATCTAC CACTTGCAGC TATTCGAGGA ACTCCGCCGC 
CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCTGCCGCCG TGGGTGCCGT GGAGGCCTCC 
TTCAAGTGCT GCAGTGGGGC CATTATCGTG CTCACCAAGT CTGGCAGGAG TGCTCACCAA 
GTGGCCAGGT ACCGCCCTCG GGCTCCTATC ATTGCCGTGA CTCGAAATCC CCAGACTGCT 
CGCCAGGCCC ATCTGTACCG TGGCATCTTC CCTGTGCTGT GTAAGGATGC CGTGCTGAAT 
GCCTGGGCTG AGGATGTCGA CCTTCGTGTA AACTTGGCCA TGGATGTTGG CAAGGCCCGA 
GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GGTGGCGCCC TGGCTCTGGA 
TTCACCAACA CCATGCGTGT AGTGCCTGTA CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035229.2
CDS	296..1891
Translation	MPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

Target ORF information:

RefSeq Version	NM_011099.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011099.3

ORF Insert Sequence:

ATGCCGAAGC CACACAGTGA AGCAGGGACT GCCTTCATTC AGACCCAGCA GCTCCATGCA 
GCCATGGCTG ACACCTTCCT GGAACACATG TGCCGCCTGG ACATTGACTC TGCCCCCATC 
ACGGCCCGCA ACACTGGCAT CATTTGTACC ATTGGGCCTG CTTCCCGATC TGTGGAGATG 
CTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGGC TGAATTTCTC TCATGGAACC 
CATGAGTACC ATGCAGAGAC CATCAAGAAT GTCCGTGAAG CCACAGAAAG CTTTGCATCT 
GATCCCATTC TCTACCGTCC TGTTGCGGTG GCTCTGGATA CAAAGGGACC TGAGATCCGG 
ACTGGACTCA TCAAGGGCAG CGGCACCGCT GAGGTGGAGC TGAAGAAGGG AGCCACTCTG 
AAGATCACCC TGGACAACGC TTACATGGAG AAGTGTGACG AGAACATCCT GTGGCTGGAC 
TACAAGAACA TCTGCAAGGT GGTGGAGGTG GGCAGCAAGA TCTACGTGGA CGATGGGCTC 
ATCTCACTGC AGGTGAAGGA GAAAGGCGCT GACTTCCTGG TGACGGAGGT GGAGAATGGT 
GGCTCCTTGG GCAGCAAGAA GGGCGTGAAC CTGCCGGGCG CTGCTGTGGA TCTCCCCGCT 
GTGTCGGAAA AGGACATCCA GGACCTGAAG TTTGGGGTGG AGCAGGATGT GGACATGGTG 
TTTGCATCTT TCATCCGCAA GGCAGCCGAC GTGCATGAAG TCAGGAAGGT GCTGGGAGAG 
AAGGGCAAGA ACATCAAGAT CATCAGCAAA ATCGAGAACC ATGAAGGCGT CCGCAGGTTT 
GATGAGATCT TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGACCT GGGCATTGAG 
ATTCCTGCAG AGAAGGTCTT CCTGGCTCAG AAGATGATGA TCGGGCGATG CAACCGAGCT 
GGGAAGCCTG TCATCTGTGC CACACAGATG CTGGAGAGCA TGATCAAGAA GCCACGCCCC 
ACCCGTGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCAGA CTGCATCATG 
CTGTCTGGAG AAACAGCCAA GGGGGACTAC CCTCTGGAGG CTGTTCGCAT GCAGCACCTG 
ATTGCCCGAG AGGCAGAGGC TGCCATCTAC CACTTGCAGC TATTCGAGGA ACTCCGCCGC 
CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCTGCCGCCG TGGGTGCCGT GGAGGCCTCC 
TTCAAGTGCT GCAGTGGGGC CATTATCGTG CTCACCAAGT CTGGCAGGAG TGCTCACCAA 
GTGGCCAGGT ACCGCCCTCG GGCTCCTATC ATTGCCGTGA CTCGAAATCC CCAGACTGCT 
CGCCAGGCCC ATCTGTACCG TGGCATCTTC CCTGTGCTGT GTAAGGATGC CGTGCTGAAT 
GCCTGGGCTG AGGATGTCGA CCTTCGTGTA AACTTGGCCA TGGATGTTGG CAAGGCCCGA 
GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GGTGGCGCCC TGGCTCTGGA 
TTCACCAACA CCATGCGTGT AGTGCCTGTA CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkm ( XM_006510855.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510918.1 Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X3, mRNA.

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CloneID	OMu15721
Clone ID Related Accession (Same CDS sequence)	XM_006510855.1 , NM_011099.3
Accession Version	XM_006510855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	pyruvate kinase PKM isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC CACACAGTGA AGCAGGGACT GCCTTCATTC AGACCCAGCA GCTCCATGCA 
GCCATGGCTG ACACCTTCCT GGAACACATG TGCCGCCTGG ACATTGACTC TGCCCCCATC 
ACGGCCCGCA ACACTGGCAT CATTTGTACC ATTGGGCCTG CTTCCCGATC TGTGGAGATG 
CTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGGC TGAATTTCTC TCATGGAACC 
CATGAGTACC ATGCAGAGAC CATCAAGAAT GTCCGTGAAG CCACAGAAAG CTTTGCATCT 
GATCCCATTC TCTACCGTCC TGTTGCGGTG GCTCTGGATA CAAAGGGACC TGAGATCCGG 
ACTGGACTCA TCAAGGGCAG CGGCACCGCT GAGGTGGAGC TGAAGAAGGG AGCCACTCTG 
AAGATCACCC TGGACAACGC TTACATGGAG AAGTGTGACG AGAACATCCT GTGGCTGGAC 
TACAAGAACA TCTGCAAGGT GGTGGAGGTG GGCAGCAAGA TCTACGTGGA CGATGGGCTC 
ATCTCACTGC AGGTGAAGGA GAAAGGCGCT GACTTCCTGG TGACGGAGGT GGAGAATGGT 
GGCTCCTTGG GCAGCAAGAA GGGCGTGAAC CTGCCGGGCG CTGCTGTGGA TCTCCCCGCT 
GTGTCGGAAA AGGACATCCA GGACCTGAAG TTTGGGGTGG AGCAGGATGT GGACATGGTG 
TTTGCATCTT TCATCCGCAA GGCAGCCGAC GTGCATGAAG TCAGGAAGGT GCTGGGAGAG 
AAGGGCAAGA ACATCAAGAT CATCAGCAAA ATCGAGAACC ATGAAGGCGT CCGCAGGTTT 
GATGAGATCT TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGACCT GGGCATTGAG 
ATTCCTGCAG AGAAGGTCTT CCTGGCTCAG AAGATGATGA TCGGGCGATG CAACCGAGCT 
GGGAAGCCTG TCATCTGTGC CACACAGATG CTGGAGAGCA TGATCAAGAA GCCACGCCCC 
ACCCGTGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCAGA CTGCATCATG 
CTGTCTGGAG AAACAGCCAA GGGGGACTAC CCTCTGGAGG CTGTTCGCAT GCAGCACCTG 
ATTGCCCGAG AGGCAGAGGC TGCCATCTAC CACTTGCAGC TATTCGAGGA ACTCCGCCGC 
CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCTGCCGCCG TGGGTGCCGT GGAGGCCTCC 
TTCAAGTGCT GCAGTGGGGC CATTATCGTG CTCACCAAGT CTGGCAGGAG TGCTCACCAA 
GTGGCCAGGT ACCGCCCTCG GGCTCCTATC ATTGCCGTGA CTCGAAATCC CCAGACTGCT 
CGCCAGGCCC ATCTGTACCG TGGCATCTTC CCTGTGCTGT GTAAGGATGC CGTGCTGAAT 
GCCTGGGCTG AGGATGTCGA CCTTCGTGTA AACTTGGCCA TGGATGTTGG CAAGGCCCGA 
GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GGTGGCGCCC TGGCTCTGGA 
TTCACCAACA CCATGCGTGT AGTGCCTGTA CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510918.1
CDS	242..1837
Translation	MPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

Target ORF information:

RefSeq Version	XM_006510855.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510855.1

ORF Insert Sequence:

ATGCCGAAGC CACACAGTGA AGCAGGGACT GCCTTCATTC AGACCCAGCA GCTCCATGCA 
GCCATGGCTG ACACCTTCCT GGAACACATG TGCCGCCTGG ACATTGACTC TGCCCCCATC 
ACGGCCCGCA ACACTGGCAT CATTTGTACC ATTGGGCCTG CTTCCCGATC TGTGGAGATG 
CTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGGC TGAATTTCTC TCATGGAACC 
CATGAGTACC ATGCAGAGAC CATCAAGAAT GTCCGTGAAG CCACAGAAAG CTTTGCATCT 
GATCCCATTC TCTACCGTCC TGTTGCGGTG GCTCTGGATA CAAAGGGACC TGAGATCCGG 
ACTGGACTCA TCAAGGGCAG CGGCACCGCT GAGGTGGAGC TGAAGAAGGG AGCCACTCTG 
AAGATCACCC TGGACAACGC TTACATGGAG AAGTGTGACG AGAACATCCT GTGGCTGGAC 
TACAAGAACA TCTGCAAGGT GGTGGAGGTG GGCAGCAAGA TCTACGTGGA CGATGGGCTC 
ATCTCACTGC AGGTGAAGGA GAAAGGCGCT GACTTCCTGG TGACGGAGGT GGAGAATGGT 
GGCTCCTTGG GCAGCAAGAA GGGCGTGAAC CTGCCGGGCG CTGCTGTGGA TCTCCCCGCT 
GTGTCGGAAA AGGACATCCA GGACCTGAAG TTTGGGGTGG AGCAGGATGT GGACATGGTG 
TTTGCATCTT TCATCCGCAA GGCAGCCGAC GTGCATGAAG TCAGGAAGGT GCTGGGAGAG 
AAGGGCAAGA ACATCAAGAT CATCAGCAAA ATCGAGAACC ATGAAGGCGT CCGCAGGTTT 
GATGAGATCT TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGACCT GGGCATTGAG 
ATTCCTGCAG AGAAGGTCTT CCTGGCTCAG AAGATGATGA TCGGGCGATG CAACCGAGCT 
GGGAAGCCTG TCATCTGTGC CACACAGATG CTGGAGAGCA TGATCAAGAA GCCACGCCCC 
ACCCGTGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCAGA CTGCATCATG 
CTGTCTGGAG AAACAGCCAA GGGGGACTAC CCTCTGGAGG CTGTTCGCAT GCAGCACCTG 
ATTGCCCGAG AGGCAGAGGC TGCCATCTAC CACTTGCAGC TATTCGAGGA ACTCCGCCGC 
CTGGCGCCCA TTACCAGCGA CCCCACAGAA GCTGCCGCCG TGGGTGCCGT GGAGGCCTCC 
TTCAAGTGCT GCAGTGGGGC CATTATCGTG CTCACCAAGT CTGGCAGGAG TGCTCACCAA 
GTGGCCAGGT ACCGCCCTCG GGCTCCTATC ATTGCCGTGA CTCGAAATCC CCAGACTGCT 
CGCCAGGCCC ATCTGTACCG TGGCATCTTC CCTGTGCTGT GTAAGGATGC CGTGCTGAAT 
GCCTGGGCTG AGGATGTCGA CCTTCGTGTA AACTTGGCCA TGGATGTTGG CAAGGCCCGA 
GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GGTGGCGCCC TGGCTCTGGA 
TTCACCAACA CCATGCGTGT AGTGCCTGTA CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkm ( XM_011242674.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240976.2 Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X1, mRNA.

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CloneID	OMu90104
Clone ID Related Accession (Same CDS sequence)	XM_011242674.2
Accession Version	XM_011242674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	pyruvate kinase PKM isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCAAC CTTTCAACTT TGCGGCTTTT GAGAAAATTT TCTTCCTTGG TTTCTCAATC 
ATCTCAAATA CCCACAGGCA AGTTAACTGC TCTTCAGGGC AAAGCATACT CCACTCTGAG 
CGATCTTTCC AAGTGAGGCC ACCAAGCCAC ACTGAGTCAA CAAAACAGCA ATATGCCATC 
AGAAGCCCAG ACCCAGAGAA CCAAAGGACT TCAGGAACCA TGCCGAAGCC ACACAGTGAA 
GCAGGGACTG CCTTCATTCA GACCCAGCAG CTCCATGCAG CCATGGCTGA CACCTTCCTG 
GAACACATGT GCCGCCTGGA CATTGACTCT GCCCCCATCA CGGCCCGCAA CACTGGCATC 
ATTTGTACCA TTGGGCCTGC TTCCCGATCT GTGGAGATGC TGAAGGAGAT GATTAAGTCT 
GGAATGAATG TGGCTCGGCT GAATTTCTCT CATGGAACCC ATGAGTACCA TGCAGAGACC 
ATCAAGAATG TCCGTGAAGC CACAGAAAGC TTTGCATCTG ATCCCATTCT CTACCGTCCT 
GTTGCGGTGG CTCTGGATAC AAAGGGACCT GAGATCCGGA CTGGACTCAT CAAGGGCAGC 
GGCACCGCTG AGGTGGAGCT GAAGAAGGGA GCCACTCTGA AGATCACCCT GGACAACGCT 
TACATGGAGA AGTGTGACGA GAACATCCTG TGGCTGGACT ACAAGAACAT CTGCAAGGTG 
GTGGAGGTGG GCAGCAAGAT CTACGTGGAC GATGGGCTCA TCTCACTGCA GGTGAAGGAG 
AAAGGCGCTG ACTTCCTGGT GACGGAGGTG GAGAATGGTG GCTCCTTGGG CAGCAAGAAG 
GGCGTGAACC TGCCGGGCGC TGCTGTGGAT CTCCCCGCTG TGTCGGAAAA GGACATCCAG 
GACCTGAAGT TTGGGGTGGA GCAGGATGTG GACATGGTGT TTGCATCTTT CATCCGCAAG 
GCAGCCGACG TGCATGAAGT CAGGAAGGTG CTGGGAGAGA AGGGCAAGAA CATCAAGATC 
ATCAGCAAAA TCGAGAACCA TGAAGGCGTC CGCAGGTTTG ATGAGATCTT GGAGGCCAGT 
GATGGGATCA TGGTGGCTCG TGGTGACCTG GGCATTGAGA TTCCTGCAGA GAAGGTCTTC 
CTGGCTCAGA AGATGATGAT CGGGCGATGC AACCGAGCTG GGAAGCCTGT CATCTGTGCC 
ACACAGATGC TGGAGAGCAT GATCAAGAAG CCACGCCCCA CCCGTGCTGA AGGCAGTGAT 
GTGGCCAATG CAGTCCTGGA TGGAGCAGAC TGCATCATGC TGTCTGGAGA AACAGCCAAG 
GGGGACTACC CTCTGGAGGC TGTTCGCATG CAGCACCTGA TTGCCCGAGA GGCAGAGGCT 
GCCATCTACC ACTTGCAGCT ATTCGAGGAA CTCCGCCGCC TGGCGCCCAT TACCAGCGAC 
CCCACAGAAG CTGCCGCCGT GGGTGCCGTG GAGGCCTCCT TCAAGTGCTG CAGTGGGGCC 
ATTATCGTGC TCACCAAGTC TGGCAGGAGT GCTCACCAAG TGGCCAGGTA CCGCCCTCGG 
GCTCCTATCA TTGCCGTGAC TCGAAATCCC CAGACTGCTC GCCAGGCCCA TCTGTACCGT 
GGCATCTTCC CTGTGCTGTG TAAGGATGCC GTGCTGAATG CCTGGGCTGA GGATGTCGAC 
CTTCGTGTAA ACTTGGCCAT GGATGTTGGC AAGGCCCGAG GCTTCTTCAA GAAGGGAGAT 
GTGGTCATTG TGCTGACCGG GTGGCGCCCT GGCTCTGGAT TCACCAACAC CATGCGTGTA 
GTGCCTGTAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240976.2
CDS	1485..3299
Translation	MTQPFNFAAFEKIFFLGFSIISNTHRQVNCSSGQSILHSERSFQVRPPSHTESTKQQYAIRSPDPENQRTSGTMPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

Target ORF information:

RefSeq Version	XM_011242674.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242674.2

ORF Insert Sequence:

ATGACTCAAC CTTTCAACTT TGCGGCTTTT GAGAAAATTT TCTTCCTTGG TTTCTCAATC 
ATCTCAAATA CCCACAGGCA AGTTAACTGC TCTTCAGGGC AAAGCATACT CCACTCTGAG 
CGATCTTTCC AAGTGAGGCC ACCAAGCCAC ACTGAGTCAA CAAAACAGCA ATATGCCATC 
AGAAGCCCAG ACCCAGAGAA CCAAAGGACT TCAGGAACCA TGCCGAAGCC ACACAGTGAA 
GCAGGGACTG CCTTCATTCA GACCCAGCAG CTCCATGCAG CCATGGCTGA CACCTTCCTG 
GAACACATGT GCCGCCTGGA CATTGACTCT GCCCCCATCA CGGCCCGCAA CACTGGCATC 
ATTTGTACCA TTGGGCCTGC TTCCCGATCT GTGGAGATGC TGAAGGAGAT GATTAAGTCT 
GGAATGAATG TGGCTCGGCT GAATTTCTCT CATGGAACCC ATGAGTACCA TGCAGAGACC 
ATCAAGAATG TCCGTGAAGC CACAGAAAGC TTTGCATCTG ATCCCATTCT CTACCGTCCT 
GTTGCGGTGG CTCTGGATAC AAAGGGACCT GAGATCCGGA CTGGACTCAT CAAGGGCAGC 
GGCACCGCTG AGGTGGAGCT GAAGAAGGGA GCCACTCTGA AGATCACCCT GGACAACGCT 
TACATGGAGA AGTGTGACGA GAACATCCTG TGGCTGGACT ACAAGAACAT CTGCAAGGTG 
GTGGAGGTGG GCAGCAAGAT CTACGTGGAC GATGGGCTCA TCTCACTGCA GGTGAAGGAG 
AAAGGCGCTG ACTTCCTGGT GACGGAGGTG GAGAATGGTG GCTCCTTGGG CAGCAAGAAG 
GGCGTGAACC TGCCGGGCGC TGCTGTGGAT CTCCCCGCTG TGTCGGAAAA GGACATCCAG 
GACCTGAAGT TTGGGGTGGA GCAGGATGTG GACATGGTGT TTGCATCTTT CATCCGCAAG 
GCAGCCGACG TGCATGAAGT CAGGAAGGTG CTGGGAGAGA AGGGCAAGAA CATCAAGATC 
ATCAGCAAAA TCGAGAACCA TGAAGGCGTC CGCAGGTTTG ATGAGATCTT GGAGGCCAGT 
GATGGGATCA TGGTGGCTCG TGGTGACCTG GGCATTGAGA TTCCTGCAGA GAAGGTCTTC 
CTGGCTCAGA AGATGATGAT CGGGCGATGC AACCGAGCTG GGAAGCCTGT CATCTGTGCC 
ACACAGATGC TGGAGAGCAT GATCAAGAAG CCACGCCCCA CCCGTGCTGA AGGCAGTGAT 
GTGGCCAATG CAGTCCTGGA TGGAGCAGAC TGCATCATGC TGTCTGGAGA AACAGCCAAG 
GGGGACTACC CTCTGGAGGC TGTTCGCATG CAGCACCTGA TTGCCCGAGA GGCAGAGGCT 
GCCATCTACC ACTTGCAGCT ATTCGAGGAA CTCCGCCGCC TGGCGCCCAT TACCAGCGAC 
CCCACAGAAG CTGCCGCCGT GGGTGCCGTG GAGGCCTCCT TCAAGTGCTG CAGTGGGGCC 
ATTATCGTGC TCACCAAGTC TGGCAGGAGT GCTCACCAAG TGGCCAGGTA CCGCCCTCGG 
GCTCCTATCA TTGCCGTGAC TCGAAATCCC CAGACTGCTC GCCAGGCCCA TCTGTACCGT 
GGCATCTTCC CTGTGCTGTG TAAGGATGCC GTGCTGAATG CCTGGGCTGA GGATGTCGAC 
CTTCGTGTAA ACTTGGCCAT GGATGTTGGC AAGGCCCGAG GCTTCTTCAA GAAGGGAGAT 
GTGGTCATTG TGCTGACCGG GTGGCGCCCT GGCTCTGGAT TCACCAACAC CATGCGTGTA 
GTGCCTGTAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkm ( XM_011242673.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240975.2 Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu90105
Clone ID Related Accession (Same CDS sequence)	XM_011242673.2
Accession Version	XM_011242673.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	pyruvate kinase PKM isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCAAC CTTTCAACTT TGCGGCTTTT GAGAAAATTT TCTTCCTTGG CTCTTCAGGG 
CAAAGCATAC TCCACTCTGA GCGATCTTTC CAAGTGAGGC CACCAAGCCA CACTGAGTCA 
ACAAAACAGC AATATGCCAT CAGAAGCCCA GACCCAGAGA ACCAAAGGAC TTCAGGAACC 
ATGCCGAAGC CACACAGTGA AGCAGGGACT GCCTTCATTC AGACCCAGCA GCTCCATGCA 
GCCATGGCTG ACACCTTCCT GGAACACATG TGCCGCCTGG ACATTGACTC TGCCCCCATC 
ACGGCCCGCA ACACTGGCAT CATTTGTACC ATTGGGCCTG CTTCCCGATC TGTGGAGATG 
CTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGGC TGAATTTCTC TCATGGAACC 
CATGAGTACC ATGCAGAGAC CATCAAGAAT GTCCGTGAAG CCACAGAAAG CTTTGCATCT 
GATCCCATTC TCTACCGTCC TGTTGCGGTG GCTCTGGATA CAAAGGGACC TGAGATCCGG 
ACTGGACTCA TCAAGGGCAG CGGCACCGCT GAGGTGGAGC TGAAGAAGGG AGCCACTCTG 
AAGATCACCC TGGACAACGC TTACATGGAG AAGTGTGACG AGAACATCCT GTGGCTGGAC 
TACAAGAACA TCTGCAAGGT GGTGGAGGTG GGCAGCAAGA TCTACGTGGA CGATGGGCTC 
ATCTCACTGC AGGTGAAGGA GAAAGGCGCT GACTTCCTGG TGACGGAGGT GGAGAATGGT 
GGCTCCTTGG GCAGCAAGAA GGGCGTGAAC CTGCCGGGCG CTGCTGTGGA TCTCCCCGCT 
GTGTCGGAAA AGGACATCCA GGACCTGAAG TTTGGGGTGG AGCAGGATGT GGACATGGTG 
TTTGCATCTT TCATCCGCAA GGCAGCCGAC GTGCATGAAG TCAGGAAGGT GCTGGGAGAG 
AAGGGCAAGA ACATCAAGAT CATCAGCAAA ATCGAGAACC ATGAAGGCGT CCGCAGGTTT 
GATGAGATCT TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGACCT GGGCATTGAG 
ATTCCTGCAG AGAAGGTCTT CCTGGCTCAG AAGATGATGA TCGGGCGATG CAACCGAGCT 
GGGAAGCCTG TCATCTGTGC CACACAGATG CTGGAGAGCA TGATCAAGAA GCCACGCCCC 
ACCCGTGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCAGA CTGCATCATG 
CTGTCTGGAG AAACAGCCAA GGGGGACTAC CCTCTGGAGG CTGTTCGCAT GCAGCACCTG 
ATAGCTCGGG AGGCTGAGGC AGCCATGTTC CACCGTCTGC TGTTTGAAGA GCTTGTGCGA 
GCCTCCAGTC ACTCCACAGA CCTCATGGAG GCCATGGCCA TGGGCAGCGT GGAGGCCTCT 
TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTCACGGAGT CTGGCAGGAG TGCTCACCAA 
GTGGCCAGGT ACCGCCCTCG GGCTCCTATC ATTGCCGTGA CTCGAAATCC CCAGACTGCT 
CGCCAGGCCC ATCTGTACCG TGGCATCTTC CCTGTGCTGT GTAAGGATGC CGTGCTGAAT 
GCCTGGGCTG AGGATGTCGA CCTTCGTGTA AACTTGGCCA TGGATGTTGG CAAGGCCCGA 
GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GGTGGCGCCC TGGCTCTGGA 
TTCACCAACA CCATGCGTGT AGTGCCTGTA CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240975.2
CDS	1485..3260
Translation	MTQPFNFAAFEKIFFLGSSGQSILHSERSFQVRPPSHTESTKQQYAIRSPDPENQRTSGTMPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRLLFEELVRASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

Target ORF information:

RefSeq Version	XM_011242673.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate kinase, muscle (Pkm), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242673.2

ORF Insert Sequence:

ATGACTCAAC CTTTCAACTT TGCGGCTTTT GAGAAAATTT TCTTCCTTGG CTCTTCAGGG 
CAAAGCATAC TCCACTCTGA GCGATCTTTC CAAGTGAGGC CACCAAGCCA CACTGAGTCA 
ACAAAACAGC AATATGCCAT CAGAAGCCCA GACCCAGAGA ACCAAAGGAC TTCAGGAACC 
ATGCCGAAGC CACACAGTGA AGCAGGGACT GCCTTCATTC AGACCCAGCA GCTCCATGCA 
GCCATGGCTG ACACCTTCCT GGAACACATG TGCCGCCTGG ACATTGACTC TGCCCCCATC 
ACGGCCCGCA ACACTGGCAT CATTTGTACC ATTGGGCCTG CTTCCCGATC TGTGGAGATG 
CTGAAGGAGA TGATTAAGTC TGGAATGAAT GTGGCTCGGC TGAATTTCTC TCATGGAACC 
CATGAGTACC ATGCAGAGAC CATCAAGAAT GTCCGTGAAG CCACAGAAAG CTTTGCATCT 
GATCCCATTC TCTACCGTCC TGTTGCGGTG GCTCTGGATA CAAAGGGACC TGAGATCCGG 
ACTGGACTCA TCAAGGGCAG CGGCACCGCT GAGGTGGAGC TGAAGAAGGG AGCCACTCTG 
AAGATCACCC TGGACAACGC TTACATGGAG AAGTGTGACG AGAACATCCT GTGGCTGGAC 
TACAAGAACA TCTGCAAGGT GGTGGAGGTG GGCAGCAAGA TCTACGTGGA CGATGGGCTC 
ATCTCACTGC AGGTGAAGGA GAAAGGCGCT GACTTCCTGG TGACGGAGGT GGAGAATGGT 
GGCTCCTTGG GCAGCAAGAA GGGCGTGAAC CTGCCGGGCG CTGCTGTGGA TCTCCCCGCT 
GTGTCGGAAA AGGACATCCA GGACCTGAAG TTTGGGGTGG AGCAGGATGT GGACATGGTG 
TTTGCATCTT TCATCCGCAA GGCAGCCGAC GTGCATGAAG TCAGGAAGGT GCTGGGAGAG 
AAGGGCAAGA ACATCAAGAT CATCAGCAAA ATCGAGAACC ATGAAGGCGT CCGCAGGTTT 
GATGAGATCT TGGAGGCCAG TGATGGGATC ATGGTGGCTC GTGGTGACCT GGGCATTGAG 
ATTCCTGCAG AGAAGGTCTT CCTGGCTCAG AAGATGATGA TCGGGCGATG CAACCGAGCT 
GGGAAGCCTG TCATCTGTGC CACACAGATG CTGGAGAGCA TGATCAAGAA GCCACGCCCC 
ACCCGTGCTG AAGGCAGTGA TGTGGCCAAT GCAGTCCTGG ATGGAGCAGA CTGCATCATG 
CTGTCTGGAG AAACAGCCAA GGGGGACTAC CCTCTGGAGG CTGTTCGCAT GCAGCACCTG 
ATAGCTCGGG AGGCTGAGGC AGCCATGTTC CACCGTCTGC TGTTTGAAGA GCTTGTGCGA 
GCCTCCAGTC ACTCCACAGA CCTCATGGAG GCCATGGCCA TGGGCAGCGT GGAGGCCTCT 
TATAAGTGTT TAGCAGCAGC TTTGATAGTT CTCACGGAGT CTGGCAGGAG TGCTCACCAA 
GTGGCCAGGT ACCGCCCTCG GGCTCCTATC ATTGCCGTGA CTCGAAATCC CCAGACTGCT 
CGCCAGGCCC ATCTGTACCG TGGCATCTTC CCTGTGCTGT GTAAGGATGC CGTGCTGAAT 
GCCTGGGCTG AGGATGTCGA CCTTCGTGTA AACTTGGCCA TGGATGTTGG CAAGGCCCGA 
GGCTTCTTCA AGAAGGGAGA TGTGGTCATT GTGCTGACCG GGTGGCGCCC TGGCTCTGGA 
TTCACCAACA CCATGCGTGT AGTGCCTGTA CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Pkm cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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