ORF » Species Summary » Danio rerio » pkma cDNA ORF clone

pkma cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following pkma gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pkma cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa37275 NM_199333.1
Latest version!		 Danio rerio pyruvate kinase M1/2a (pkma), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa40050 XM_009293364.2
Latest version!		 Danio rerio pyruvate kinase, muscle, a (pkma), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
View Old Accession Versions >>
ODa40050 XM_009293364.1 Danio rerio pyruvate kinase, muscle, a (pkma), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    pkma ( NM_199333.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_955365.1 Danio rerio pyruvate kinase M1/2a (pkma), mRNA.
    Price & Availability↑
    CloneID ODa37275
    Clone ID Related Accession (Same CDS sequence) NM_199333.1
    Accession Version NM_199333.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-22
    Organism Danio rerio(zebrafish)
    Product pyruvate kinase PKM
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045421.1. On Jul 6, 2005 this sequence version replaced XM_686210.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC045421.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCTCAAA CTAAAGCTCA GGATATGGGC TCTGCCTTCA TCCAGACGCA GCAGCTCAAT 
    GCTGCCATGG CCGACACTTT CCTGGAGCAC ATGTGTCTGC TGGACATCGA CTCCGAGCCC 
    ACCATCGCTC GCAACACTGG AATCATCTGC ACCATCGGAC CGGCCTCCCG CTCAGTGGAC 
    ACACTGAAGG AGATGATCAA GTCTGGCATG AACATCGCTC GCATGAACTT CTCTCACGGC 
    TCACACGAGT ATCATGGAGA AACTATTAAA AATGTACGTG AAGCTTGTGC CAGCTTTGAG 
    CCGGGCAGCA TCCAGTACAG GCCAGTGGGC ATCGCCCTCG ACACCAAAGG ACCAGAAATC 
    CGAACTGGGC TCATTAAAGG GAGTGGCACA GCTGAGGTGG AGCTGAAGAA GGGAAATAAG 
    ATCAAGGTGA CCTTAGATGA CTCCTTCATG GAGAACTGTG ATGAAGATAC CCTCTGGCTG 
    GACTACAAGA ACATCACCAA GGTGGTGGAG GTGGGCAGTA AAGTCTACAT CGATGACGGA 
    CTCATCTCTC TCCAGGTGCT GCAGATCGGG TCTGACTATC TGATCTGTGA GATTGAGAAT 
    GGAGGATCTC TGGGCAGTAA GAAGGGTGTG AATCTGCCGG GAGCCGCAGT GGATCTGCCT 
    GCAGTCTCAG AGAAGGACAT CAAGGATCTG CAGTTCGGTG TGGAGATGGG AGTCGATATG 
    ATCTTCGCCT CCTTCATCCG CAAGGCCGCT GATGTGCAGG CAGTCCGAAA AGTGCTGGGA 
    GAAAAGGGCA AAAACATCAA GATCATCAGC AAGCTGGAGA ACCATGAGGG AGTGCGCAGG 
    TTTGATGAGA TTATGGAGGC CAGCGACGGC ATCATGGTGG CTCGTGGTGA TCTAGGAATT 
    GAAATCCCCA CAGAAAAGGT GTTTCTGGCT CAGAAGATGA TGATTGGTCG TTGCAACAAG 
    GCAGGAAAGC CAATCATCTG TGCCACACAG ATGTTGGAGA GCATGATCAA GAAGCCTCGT 
    CCTACACGTG CGGAGGGCAG TGATGTGGCC AATGCAGTTC TGGACGGAGC CGACTGCATT 
    ATGCTGAGCG GAGAGACCGC TAAAGGAGAT TATCCTCTGG AGGCCGTCCG CACACAGCAC 
    ATGATTGCCC GTGAGGCAGA GGCCGCCACC TTCCACCGGC AGCTGTTTGA GGGTCTGAGA 
    CGCTCCTCTG TTCTGACCCG TGACCCTTCA GATGCAGTCG CTGTGGGAGC CGTCGAGGCT 
    TCCTTCAAAT GCTGCGCCAG CGGCATCATC ATACTCACCA AAACCGGCAG GTCTGCTCAT 
    CTGATCTCTC GGTATCGTCC TCGCGCTCCA ATCCTAGCGG TGACTCGTAA TGAACAGACA 
    GCTCGACAGG CTCATCTTTA CCGTGGTATT TTCCCCATAT ACTACAACAG CCCATCTAAT 
    GATGTGTGGG CCGAGGACGT GGACCTGAGG GTCAACTTCG CCATGGATGT CGGTAAAGCT 
    CGTGGATTCT TCAAGGCTGG TGATGTGGTG ATCGTTCTGA CTGGCTGGCG TCCCGGCTCT 
    GGATACACCA ACACCATGCG TGTCGTCCCA GTGCCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_955365.1
    CDS56..1654
    Translation

    Target ORF information:

    RefSeq Version NM_199333.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pyruvate kinase M1/2a (pkma), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_199333.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCTCAAA CTAAAGCTCA GGATATGGGC TCTGCCTTCA TCCAGACGCA GCAGCTCAAT 
    GCTGCCATGG CCGACACTTT CCTGGAGCAC ATGTGTCTGC TGGACATCGA CTCCGAGCCC 
    ACCATCGCTC GCAACACTGG AATCATCTGC ACCATCGGAC CGGCCTCCCG CTCAGTGGAC 
    ACACTGAAGG AGATGATCAA GTCTGGCATG AACATCGCTC GCATGAACTT CTCTCACGGC 
    TCACACGAGT ATCATGGAGA AACTATTAAA AATGTACGTG AAGCTTGTGC CAGCTTTGAG 
    CCGGGCAGCA TCCAGTACAG GCCAGTGGGC ATCGCCCTCG ACACCAAAGG ACCAGAAATC 
    CGAACTGGGC TCATTAAAGG GAGTGGCACA GCTGAGGTGG AGCTGAAGAA GGGAAATAAG 
    ATCAAGGTGA CCTTAGATGA CTCCTTCATG GAGAACTGTG ATGAAGATAC CCTCTGGCTG 
    GACTACAAGA ACATCACCAA GGTGGTGGAG GTGGGCAGTA AAGTCTACAT CGATGACGGA 
    CTCATCTCTC TCCAGGTGCT GCAGATCGGG TCTGACTATC TGATCTGTGA GATTGAGAAT 
    GGAGGATCTC TGGGCAGTAA GAAGGGTGTG AATCTGCCGG GAGCCGCAGT GGATCTGCCT 
    GCAGTCTCAG AGAAGGACAT CAAGGATCTG CAGTTCGGTG TGGAGATGGG AGTCGATATG 
    ATCTTCGCCT CCTTCATCCG CAAGGCCGCT GATGTGCAGG CAGTCCGAAA AGTGCTGGGA 
    GAAAAGGGCA AAAACATCAA GATCATCAGC AAGCTGGAGA ACCATGAGGG AGTGCGCAGG 
    TTTGATGAGA TTATGGAGGC CAGCGACGGC ATCATGGTGG CTCGTGGTGA TCTAGGAATT 
    GAAATCCCCA CAGAAAAGGT GTTTCTGGCT CAGAAGATGA TGATTGGTCG TTGCAACAAG 
    GCAGGAAAGC CAATCATCTG TGCCACACAG ATGTTGGAGA GCATGATCAA GAAGCCTCGT 
    CCTACACGTG CGGAGGGCAG TGATGTGGCC AATGCAGTTC TGGACGGAGC CGACTGCATT 
    ATGCTGAGCG GAGAGACCGC TAAAGGAGAT TATCCTCTGG AGGCCGTCCG CACACAGCAC 
    ATGATTGCCC GTGAGGCAGA GGCCGCCACC TTCCACCGGC AGCTGTTTGA GGGTCTGAGA 
    CGCTCCTCTG TTCTGACCCG TGACCCTTCA GATGCAGTCG CTGTGGGAGC CGTCGAGGCT 
    TCCTTCAAAT GCTGCGCCAG CGGCATCATC ATACTCACCA AAACCGGCAG GTCTGCTCAT 
    CTGATCTCTC GGTATCGTCC TCGCGCTCCA ATCCTAGCGG TGACTCGTAA TGAACAGACA 
    GCTCGACAGG CTCATCTTTA CCGTGGTATT TTCCCCATAT ACTACAACAG CCCATCTAAT 
    GATGTGTGGG CCGAGGACGT GGACCTGAGG GTCAACTTCG CCATGGATGT CGGTAAAGCT 
    CGTGGATTCT TCAAGGCTGG TGATGTGGTG ATCGTTCTGA CTGGCTGGCG TCCCGGCTCT 
    GGATACACCA ACACCATGCG TGTCGTCCCA GTGCCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pkma ( XM_009293364.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291639.1 Danio rerio pyruvate kinase, muscle, a (pkma), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa40050
    Clone ID Related Accession (Same CDS sequence) XM_009293364.1 , XM_009293364.2
    Accession Version XM_009293364.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pyruvate kinase PKM isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCTCAAA CTAAAGCTCA GGATATGGGC TCTGCCTTCA TCCAGACGCA GCAGCTCAAT 
    GCTGCCATGG CCGACACTTT CCTGGAGCAC ATGTGTCTGC TGGACATCGA CTCCGAGCCC 
    ACCATAGCTC GCAACACTGG AATCATCTGC ACCATCGGAC CGGCCTCCCG CTCAGTGGAC 
    ACACTGAAGG AGATGATCAA GTCTGGCATG AACATCGCTC GCATGAACTT CTCTCACGGC 
    TCACACGAGT ATCATGGAGA AACTATTAAA AATGTACGTG AGGCTTGTGC CAGCTTTGAG 
    CCGGGCAGCA TCCAGTACAG GCCAGTGGGC ATCGCCCTCG ACACCAAAGG ACCAGAAATC 
    CGAACTGGGC TCATTAAAGG GAGTGGCACA GCTGAGGTGG AGCTGAAGAA GGGAAATAAG 
    ATCAAGGTGA CCTTAGATGA CTCCTTCATG GAGAACTGTG ATGAAGATAC CCTCTGGCTG 
    GACTACAAGA ACATCACCAA GGTGGTGGAG GTGGGCAGTA AAGTCTACAT CGATGACGGA 
    CTCATCTCTC TCCAGGTGCT GCAGATCGGG TCTGACTATC TGATCTGTGA GATTGAGAAT 
    GGAGGATCTC TGGGCAGTAA GAAGGGTGTG AATCTGCCGG GAGCCGCAGT GGATCTGCCT 
    GCAGTCTCAG AGAAGGACAT CAAGGATCTG CAGTTCGGTG TGGAGATGGG AGTCGACATG 
    ATCTTCGCCT CCTTCATCCG CAAGGCCGCT GATGTGCAGG CAGTCCGAAA AGTGCTGGGA 
    GAAAAGGGCA AAAACATCAA GATCATCAGC AAGCTGGAGA ACCATGAGGG AGTGCGCAGG 
    TTTGATGAGA TTATGGAGGC CAGCGACGGC ATCATGGTGG CTCGTGGTGA TCTAGGAATT 
    GAAATCCCCA CGGAAAAGGT GTTTCTGGCT CAGAAGATGA TGATTGGTCG TTGCAACAAG 
    GCAGGAAAGC CAATCATCTG TGCCACACAG ATGTTGGAGA GCATGATCAA GAAGCCTCGT 
    CCTACACGTG CGGAGGGCAG TGATGTGGCC AATGCAGTTC TGGACGGAGC CGACTGCATT 
    ATGCTGAGCG GAGAGACCGC TAAAGGAGAT TATCCTCTGG AGGCCGTCCG CACACAGCAC 
    ATGATCGCTC GGGAGGCAGA GGCTGCCATG TTTCATCGGC AGGTGTTTGA GGACCTGCGG 
    CGCTGTTTGC CACTCTCCAC CGACCCCGCC GAGGCCATCG CCATCGGGGC AGTGGAGGCC 
    TCCTTTAAAA TCCTGTCCTC AGCGTTTATA GTGCTCACTG GGTCCGGCAG GTCTGCGCAT 
    CTGATCTCTC GGTATCGTCC TCGCGCTCCA ATCCTAGCGG TGACTCGTAA TGAACAGACG 
    GCTCGACAGG CTCATCTTTA CCGTGGTATT TTCCCCATAT ACTACAACAG CCCATCTAAT 
    GATGTGTGGG CCGAGGACGT GGACCTGAGG GTCAACTTCG CCATGGATGT CGGTAAAGCT 
    CGTGGATTCT TCAAGGCTGG TGATGTGGTG ATCGTTCTGA CTGGCTGGCG TCCCGGCTCT 
    GGATACACCA ACACCATGCG TGTCGTCCCA GTGCCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291639.1
    CDS125..1723
    Translation

    Target ORF information:

    RefSeq Version XM_009293364.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pyruvate kinase, muscle, a (pkma), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009293364.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCTCAAA CTAAAGCTCA GGATATGGGC TCTGCCTTCA TCCAGACGCA GCAGCTCAAT 
    GCTGCCATGG CCGACACTTT CCTGGAGCAC ATGTGTCTGC TGGACATCGA CTCCGAGCCC 
    ACCATAGCTC GCAACACTGG AATCATCTGC ACCATCGGAC CGGCCTCCCG CTCAGTGGAC 
    ACACTGAAGG AGATGATCAA GTCTGGCATG AACATCGCTC GCATGAACTT CTCTCACGGC 
    TCACACGAGT ATCATGGAGA AACTATTAAA AATGTACGTG AGGCTTGTGC CAGCTTTGAG 
    CCGGGCAGCA TCCAGTACAG GCCAGTGGGC ATCGCCCTCG ACACCAAAGG ACCAGAAATC 
    CGAACTGGGC TCATTAAAGG GAGTGGCACA GCTGAGGTGG AGCTGAAGAA GGGAAATAAG 
    ATCAAGGTGA CCTTAGATGA CTCCTTCATG GAGAACTGTG ATGAAGATAC CCTCTGGCTG 
    GACTACAAGA ACATCACCAA GGTGGTGGAG GTGGGCAGTA AAGTCTACAT CGATGACGGA 
    CTCATCTCTC TCCAGGTGCT GCAGATCGGG TCTGACTATC TGATCTGTGA GATTGAGAAT 
    GGAGGATCTC TGGGCAGTAA GAAGGGTGTG AATCTGCCGG GAGCCGCAGT GGATCTGCCT 
    GCAGTCTCAG AGAAGGACAT CAAGGATCTG CAGTTCGGTG TGGAGATGGG AGTCGACATG 
    ATCTTCGCCT CCTTCATCCG CAAGGCCGCT GATGTGCAGG CAGTCCGAAA AGTGCTGGGA 
    GAAAAGGGCA AAAACATCAA GATCATCAGC AAGCTGGAGA ACCATGAGGG AGTGCGCAGG 
    TTTGATGAGA TTATGGAGGC CAGCGACGGC ATCATGGTGG CTCGTGGTGA TCTAGGAATT 
    GAAATCCCCA CGGAAAAGGT GTTTCTGGCT CAGAAGATGA TGATTGGTCG TTGCAACAAG 
    GCAGGAAAGC CAATCATCTG TGCCACACAG ATGTTGGAGA GCATGATCAA GAAGCCTCGT 
    CCTACACGTG CGGAGGGCAG TGATGTGGCC AATGCAGTTC TGGACGGAGC CGACTGCATT 
    ATGCTGAGCG GAGAGACCGC TAAAGGAGAT TATCCTCTGG AGGCCGTCCG CACACAGCAC 
    ATGATCGCTC GGGAGGCAGA GGCTGCCATG TTTCATCGGC AGGTGTTTGA GGACCTGCGG 
    CGCTGTTTGC CACTCTCCAC CGACCCCGCC GAGGCCATCG CCATCGGGGC AGTGGAGGCC 
    TCCTTTAAAA TCCTGTCCTC AGCGTTTATA GTGCTCACTG GGTCCGGCAG GTCTGCGCAT 
    CTGATCTCTC GGTATCGTCC TCGCGCTCCA ATCCTAGCGG TGACTCGTAA TGAACAGACG 
    GCTCGACAGG CTCATCTTTA CCGTGGTATT TTCCCCATAT ACTACAACAG CCCATCTAAT 
    GATGTGTGGG CCGAGGACGT GGACCTGAGG GTCAACTTCG CCATGGATGT CGGTAAAGCT 
    CGTGGATTCT TCAAGGCTGG TGATGTGGTG ATCGTTCTGA CTGGCTGGCG TCCCGGCTCT 
    GGATACACCA ACACCATGCG TGTCGTCCCA GTGCCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pkma ( XM_009293364.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291639.1 Danio rerio pyruvate kinase, muscle, a (pkma), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa40050
    Clone ID Related Accession (Same CDS sequence) XM_009293364.1 , XM_009293364.2
    Accession Version XM_009293364.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pyruvate kinase PKM isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293364.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCTCAAA CTAAAGCTCA GGATATGGGC TCTGCCTTCA TCCAGACGCA GCAGCTCAAT 
    GCTGCCATGG CCGACACTTT CCTGGAGCAC ATGTGTCTGC TGGACATCGA CTCCGAGCCC 
    ACCATAGCTC GCAACACTGG AATCATCTGC ACCATCGGAC CGGCCTCCCG CTCAGTGGAC 
    ACACTGAAGG AGATGATCAA GTCTGGCATG AACATCGCTC GCATGAACTT CTCTCACGGC 
    TCACACGAGT ATCATGGAGA AACTATTAAA AATGTACGTG AGGCTTGTGC CAGCTTTGAG 
    CCGGGCAGCA TCCAGTACAG GCCAGTGGGC ATCGCCCTCG ACACCAAAGG ACCAGAAATC 
    CGAACTGGGC TCATTAAAGG GAGTGGCACA GCTGAGGTGG AGCTGAAGAA GGGAAATAAG 
    ATCAAGGTGA CCTTAGATGA CTCCTTCATG GAGAACTGTG ATGAAGATAC CCTCTGGCTG 
    GACTACAAGA ACATCACCAA GGTGGTGGAG GTGGGCAGTA AAGTCTACAT CGATGACGGA 
    CTCATCTCTC TCCAGGTGCT GCAGATCGGG TCTGACTATC TGATCTGTGA GATTGAGAAT 
    GGAGGATCTC TGGGCAGTAA GAAGGGTGTG AATCTGCCGG GAGCCGCAGT GGATCTGCCT 
    GCAGTCTCAG AGAAGGACAT CAAGGATCTG CAGTTCGGTG TGGAGATGGG AGTCGACATG 
    ATCTTCGCCT CCTTCATCCG CAAGGCCGCT GATGTGCAGG CAGTCCGAAA AGTGCTGGGA 
    GAAAAGGGCA AAAACATCAA GATCATCAGC AAGCTGGAGA ACCATGAGGG AGTGCGCAGG 
    TTTGATGAGA TTATGGAGGC CAGCGACGGC ATCATGGTGG CTCGTGGTGA TCTAGGAATT 
    GAAATCCCCA CGGAAAAGGT GTTTCTGGCT CAGAAGATGA TGATTGGTCG TTGCAACAAG 
    GCAGGAAAGC CAATCATCTG TGCCACACAG ATGTTGGAGA GCATGATCAA GAAGCCTCGT 
    CCTACACGTG CGGAGGGCAG TGATGTGGCC AATGCAGTTC TGGACGGAGC CGACTGCATT 
    ATGCTGAGCG GAGAGACCGC TAAAGGAGAT TATCCTCTGG AGGCCGTCCG CACACAGCAC 
    ATGATCGCTC GGGAGGCAGA GGCTGCCATG TTTCATCGGC AGGTGTTTGA GGACCTGCGG 
    CGCTGTTTGC CACTCTCCAC CGACCCCGCC GAGGCCATCG CCATCGGGGC AGTGGAGGCC 
    TCCTTTAAAA TCCTGTCCTC AGCGTTTATA GTGCTCACTG GGTCCGGCAG GTCTGCGCAT 
    CTGATCTCTC GGTATCGTCC TCGCGCTCCA ATCCTAGCGG TGACTCGTAA TGAACAGACG 
    GCTCGACAGG CTCATCTTTA CCGTGGTATT TTCCCCATAT ACTACAACAG CCCATCTAAT 
    GATGTGTGGG CCGAGGACGT GGACCTGAGG GTCAACTTCG CCATGGATGT CGGTAAAGCT 
    CGTGGATTCT TCAAGGCTGG TGATGTGGTG ATCGTTCTGA CTGGCTGGCG TCCCGGCTCT 
    GGATACACCA ACACCATGCG TGTCGTCCCA GTGCCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291639.1
    CDS169..1767
    Translation

    Target ORF information:

    RefSeq Version XM_009293364.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pyruvate kinase, muscle, a (pkma), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009293364.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCTCAAA CTAAAGCTCA GGATATGGGC TCTGCCTTCA TCCAGACGCA GCAGCTCAAT 
    GCTGCCATGG CCGACACTTT CCTGGAGCAC ATGTGTCTGC TGGACATCGA CTCCGAGCCC 
    ACCATAGCTC GCAACACTGG AATCATCTGC ACCATCGGAC CGGCCTCCCG CTCAGTGGAC 
    ACACTGAAGG AGATGATCAA GTCTGGCATG AACATCGCTC GCATGAACTT CTCTCACGGC 
    TCACACGAGT ATCATGGAGA AACTATTAAA AATGTACGTG AGGCTTGTGC CAGCTTTGAG 
    CCGGGCAGCA TCCAGTACAG GCCAGTGGGC ATCGCCCTCG ACACCAAAGG ACCAGAAATC 
    CGAACTGGGC TCATTAAAGG GAGTGGCACA GCTGAGGTGG AGCTGAAGAA GGGAAATAAG 
    ATCAAGGTGA CCTTAGATGA CTCCTTCATG GAGAACTGTG ATGAAGATAC CCTCTGGCTG 
    GACTACAAGA ACATCACCAA GGTGGTGGAG GTGGGCAGTA AAGTCTACAT CGATGACGGA 
    CTCATCTCTC TCCAGGTGCT GCAGATCGGG TCTGACTATC TGATCTGTGA GATTGAGAAT 
    GGAGGATCTC TGGGCAGTAA GAAGGGTGTG AATCTGCCGG GAGCCGCAGT GGATCTGCCT 
    GCAGTCTCAG AGAAGGACAT CAAGGATCTG CAGTTCGGTG TGGAGATGGG AGTCGACATG 
    ATCTTCGCCT CCTTCATCCG CAAGGCCGCT GATGTGCAGG CAGTCCGAAA AGTGCTGGGA 
    GAAAAGGGCA AAAACATCAA GATCATCAGC AAGCTGGAGA ACCATGAGGG AGTGCGCAGG 
    TTTGATGAGA TTATGGAGGC CAGCGACGGC ATCATGGTGG CTCGTGGTGA TCTAGGAATT 
    GAAATCCCCA CGGAAAAGGT GTTTCTGGCT CAGAAGATGA TGATTGGTCG TTGCAACAAG 
    GCAGGAAAGC CAATCATCTG TGCCACACAG ATGTTGGAGA GCATGATCAA GAAGCCTCGT 
    CCTACACGTG CGGAGGGCAG TGATGTGGCC AATGCAGTTC TGGACGGAGC CGACTGCATT 
    ATGCTGAGCG GAGAGACCGC TAAAGGAGAT TATCCTCTGG AGGCCGTCCG CACACAGCAC 
    ATGATCGCTC GGGAGGCAGA GGCTGCCATG TTTCATCGGC AGGTGTTTGA GGACCTGCGG 
    CGCTGTTTGC CACTCTCCAC CGACCCCGCC GAGGCCATCG CCATCGGGGC AGTGGAGGCC 
    TCCTTTAAAA TCCTGTCCTC AGCGTTTATA GTGCTCACTG GGTCCGGCAG GTCTGCGCAT 
    CTGATCTCTC GGTATCGTCC TCGCGCTCCA ATCCTAGCGG TGACTCGTAA TGAACAGACG 
    GCTCGACAGG CTCATCTTTA CCGTGGTATT TTCCCCATAT ACTACAACAG CCCATCTAAT 
    GATGTGTGGG CCGAGGACGT GGACCTGAGG GTCAACTTCG CCATGGATGT CGGTAAAGCT 
    CGTGGATTCT TCAAGGCTGG TGATGTGGTG ATCGTTCTGA CTGGCTGGCG TCCCGGCTCT 
    GGATACACCA ACACCATGCG TGTCGTCCCA GTGCCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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