ATP5PF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP5PF
Entrez Gene ID	522
Full Name	ATP synthase peripheral stalk subunit F6
Synonyms	ATP5,ATP5A,ATP5J,ATPM,CF6,F6
General protein information	Preferred Names ATP synthase peripheral stalk subunit F6 Names ATP synthase-coupling factor 6, mitochondrial ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 ATPase subunit F6 coupling factor 6 mitochondrial ATP synthase, coupling factor 6 mitochondrial ATP synthase, subunit F6 mitochondrial ATPase coupling factor 6 proliferation-inducing protein 36
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 21 Map Location: 21q21.3
Summary	Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes. [provided by RefSeq, Feb 2016].
Disorder MIM:	603152

mRNA and Protein(s)

mRNA	Protein	Name
XM_005260992.3	XP_005261049.1	ATP synthase-coupling factor 6, mitochondrial isoform X1
NM_001003703.1	NP_001003703.1	ATP synthase-coupling factor 6, mitochondrial isoform a precursor
NM_001003696.1	NP_001003696.1	ATP synthase-coupling factor 6, mitochondrial isoform a precursor
NM_001320267.1	NP_001307196.1	ATP synthase-coupling factor 6, mitochondrial isoform a precursor
NM_001320266.1	NP_001307195.1	ATP synthase-coupling factor 6, mitochondrial isoform a precursor
NM_001003701.1	NP_001003701.1	ATP synthase-coupling factor 6, mitochondrial isoform b precursor
NM_001003697.1	NP_001003697.1	ATP synthase-coupling factor 6, mitochondrial isoform a precursor
NM_001685.4	NP_001676.2	ATP synthase-coupling factor 6, mitochondrial isoform a precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ATP5J	NP_777142.1
Gallus gallus (chicken)	ATP5J	XP_001231639.1
Danio rerio (zebrafish)	atp5j	NP_998472.1
Homo sapiens (human)	ATP5J	NP_001003701.1
Pan troglodytes (chimpanzee)	ATP5J	XP_001158570.1
	ATP5J	XP_001105213.2
Xenopus tropicalis (tropical clawed frog)	atp5j	NP_001017042.1
Mus musculus (house mouse)	Atp5j	NP_058035.1
Canis lupus familiaris (dog)	ATP5J	XP_003434019.1
Rattus norvegicus (Norway rat)	Atp5j	NP_446054.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.
Floyd BJ, Wilkerson EM, Veling MT, Minogue CE, Xia C, Beebe ET, Wrobel RL, Cho H, Kremer LS, Alston CL, Gromek KA, Dolan BK, Ulbrich A, Stefely JA, Bohl SL, Werner KM, Jochem A, Westphall MS, Rensvold JW, Taylor RW, Prokisch H, Kim JP, Coon JJ, Pagliarini DJ
Molecular cell63(4)621-632(2016 Aug)

Mitochondrial Inhibitory Factor Protein 1 Functions as an Endogenous Inhibitor for Coupling Factor 6.
Kawai M, Osanai T, Tanaka M, Magota K, Tomita H, Okumura K
Journal of cellular biochemistry117(7)1680-7(2016 07)

Over-expression of the ATP5J gene correlates with cell migration and 5-fluorouracil sensitivity in colorectal cancer.
Zhu H, Chen L, Zhou W, Huang Z, Hu J, Dai S, Wang X, Huang X, He C
PloS one8(10)e76846(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CF6-induced increase in apoptotic cells was blocked by immature or mature IF1, being accompanied by protein kinase B (PKB) phosphorylation. IF1 antagonizes the pro-apoptotic action of CF6 by relief of intracellular acidification and resultant phosphorylation of PKB.
Title: Mitochondrial Inhibitory Factor Protein 1 Functions as an Endogenous Inhibitor for Coupling Factor 6.

Over-expression of the ATP5J gene correlates with cell migration and 5-fluorouracil sensitivity in colorectal cancer.
Title: Over-expression of the ATP5J gene correlates with cell migration and 5-fluorouracil sensitivity in colorectal cancer.

CF6 plays a crucial role in the development of insulin resistance and hypertension
Title: Coupling factor 6-induced activation of ecto-F1F(o) complex induces insulin resistance, mild glucose intolerance and elevated blood pressure in mice.

The phenotypic range of retinal, peripheral and central nervous system disease expression is characterized in a single family with NARP syndrome from the ATPase 6 m.8993T>C mtDNA point mutation.
Title: Heterogeneous patterns of tissue injury in NARP syndrome.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following ATP5PF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP5PF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27076	NM_001320266.1 Latest version!	Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu27076	NM_001003703.1 Latest version!	Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu27076	NM_001320267.1 Latest version!	Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu27076	NM_001003697.1 Latest version!	Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu27076	NM_001003696.1 Latest version!	Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu27076	NM_001685.4 Latest version!	Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu27076	XM_005260992.3 Latest version!	Homo sapiens ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (ATP5J), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu26650	NM_001003701.1 Latest version!	Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP5PF ( NM_001003703.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001003703.1 Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27076
Clone ID Related Accession (Same CDS sequence)	XM_005260992.3 , NM_001003703.1 , NM_001003696.1 , NM_001320267.1 , NM_001320266.1 , NM_001003697.1 , NM_001685.4
Accession Version	NM_001003703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	ATP synthase-coupling factor 6, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC001178.1, BI093033.1, BQ268216.1, BC066310.1 and AI085837.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, 3, 4, 6 and 7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AV721945.1, SRR1163657.396030.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATTCTTC AGAGGCTCTT CAGGTTCTCC TCTGTCATTC GGTCAGCCGT CTCAGTCCAT 
TTGCGGAGGA ACATTGGTGT TACAGCAGTG GCATTTAATA AGGAACTTGA TCCTATACAG 
AAACTCTTTG TGGACAAGAT TAGAGAATAC AAATCTAAGC GACAGACATC TGGAGGACCT 
GTTGATGCTA GTTCAGAGTA TCAGCAAGAG CTGGAGAGGG AGCTTTTTAA GCTCAAGCAA 
ATGTTTGGTA ATGCAGACAT GAATACATTT CCCACCTTCA AATTTGAAGA TCCCAAATTT 
GAAGTCATCG AAAAACCCCA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003703.1
CDS	810..1136
Translation	MILQRLFRFSSVIRSAVSVHLRRNIGVTAVAFNKELDPIQKLFVDKIREYKSKRQTSGGPVDASSEYQQELERELFKLKQMFGNADMNTFPTFKFEDPKFEVIEKPQA

Target ORF information:

RefSeq Version	NM_001003703.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003703.1

ORF Insert Sequence:

ATGATTCTTC AGAGGCTCTT CAGGTTCTCC TCTGTCATTC GGTCAGCCGT CTCAGTCCAT 
TTGCGGAGGA ACATTGGTGT TACAGCAGTG GCATTTAATA AGGAACTTGA TCCTATACAG 
AAACTCTTTG TGGACAAGAT TAGAGAATAC AAATCTAAGC GACAGACATC TGGAGGACCT 
GTTGATGCTA GTTCAGAGTA TCAGCAAGAG CTGGAGAGGG AGCTTTTTAA GCTCAAGCAA 
ATGTTTGGTA ATGCAGACAT GAATACATTT CCCACCTTCA AATTTGAAGA TCCCAAATTT 
GAAGTCATCG AAAAACCCCA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP5PF ( NM_001003696.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001003696.1 Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu27076
Clone ID Related Accession (Same CDS sequence)	XM_005260992.3 , NM_001003703.1 , NM_001003696.1 , NM_001320267.1 , NM_001320266.1 , NM_001003697.1 , NM_001685.4
Accession Version	NM_001003696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	ATP synthase-coupling factor 6, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC001178.1, BU174560.1, BC066310.1 and AI085837.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, 4, 6 and 7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.443260.1, AU123234.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATTCTTC AGAGGCTCTT CAGGTTCTCC TCTGTCATTC GGTCAGCCGT CTCAGTCCAT 
TTGCGGAGGA ACATTGGTGT TACAGCAGTG GCATTTAATA AGGAACTTGA TCCTATACAG 
AAACTCTTTG TGGACAAGAT TAGAGAATAC AAATCTAAGC GACAGACATC TGGAGGACCT 
GTTGATGCTA GTTCAGAGTA TCAGCAAGAG CTGGAGAGGG AGCTTTTTAA GCTCAAGCAA 
ATGTTTGGTA ATGCAGACAT GAATACATTT CCCACCTTCA AATTTGAAGA TCCCAAATTT 
GAAGTCATCG AAAAACCCCA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003696.1
CDS	348..674
Translation	MILQRLFRFSSVIRSAVSVHLRRNIGVTAVAFNKELDPIQKLFVDKIREYKSKRQTSGGPVDASSEYQQELERELFKLKQMFGNADMNTFPTFKFEDPKFEVIEKPQA

Target ORF information:

RefSeq Version	NM_001003696.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003696.1

ORF Insert Sequence:

ATGATTCTTC AGAGGCTCTT CAGGTTCTCC TCTGTCATTC GGTCAGCCGT CTCAGTCCAT 
TTGCGGAGGA ACATTGGTGT TACAGCAGTG GCATTTAATA AGGAACTTGA TCCTATACAG 
AAACTCTTTG TGGACAAGAT TAGAGAATAC AAATCTAAGC GACAGACATC TGGAGGACCT 
GTTGATGCTA GTTCAGAGTA TCAGCAAGAG CTGGAGAGGG AGCTTTTTAA GCTCAAGCAA 
ATGTTTGGTA ATGCAGACAT GAATACATTT CCCACCTTCA AATTTGAAGA TCCCAAATTT 
GAAGTCATCG AAAAACCCCA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP5PF ( NM_001320267.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307196.1 Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu27076
Clone ID Related Accession (Same CDS sequence)	XM_005260992.3 , NM_001003703.1 , NM_001003696.1 , NM_001320267.1 , NM_001320266.1 , NM_001003697.1 , NM_001685.4
Accession Version	NM_001320267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	ATP synthase-coupling factor 6, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC001178.1, HY064673.1, BQ268216.1, CN260182.1, HY146471.1 and AI085837.1. Transcript Variant: This variant (7) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, 4, 6 and 7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.157429.1, SRR1163658.228465.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATTCTTC AGAGGCTCTT CAGGTTCTCC TCTGTCATTC GGTCAGCCGT CTCAGTCCAT 
TTGCGGAGGA ACATTGGTGT TACAGCAGTG GCATTTAATA AGGAACTTGA TCCTATACAG 
AAACTCTTTG TGGACAAGAT TAGAGAATAC AAATCTAAGC GACAGACATC TGGAGGACCT 
GTTGATGCTA GTTCAGAGTA TCAGCAAGAG CTGGAGAGGG AGCTTTTTAA GCTCAAGCAA 
ATGTTTGGTA ATGCAGACAT GAATACATTT CCCACCTTCA AATTTGAAGA TCCCAAATTT 
GAAGTCATCG AAAAACCCCA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307196.1
CDS	1330..1656
Translation	MILQRLFRFSSVIRSAVSVHLRRNIGVTAVAFNKELDPIQKLFVDKIREYKSKRQTSGGPVDASSEYQQELERELFKLKQMFGNADMNTFPTFKFEDPKFEVIEKPQA

Target ORF information:

RefSeq Version	NM_001320267.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320267.1

ORF Insert Sequence:

ATGATTCTTC AGAGGCTCTT CAGGTTCTCC TCTGTCATTC GGTCAGCCGT CTCAGTCCAT 
TTGCGGAGGA ACATTGGTGT TACAGCAGTG GCATTTAATA AGGAACTTGA TCCTATACAG 
AAACTCTTTG TGGACAAGAT TAGAGAATAC AAATCTAAGC GACAGACATC TGGAGGACCT 
GTTGATGCTA GTTCAGAGTA TCAGCAAGAG CTGGAGAGGG AGCTTTTTAA GCTCAAGCAA 
ATGTTTGGTA ATGCAGACAT GAATACATTT CCCACCTTCA AATTTGAAGA TCCCAAATTT 
GAAGTCATCG AAAAACCCCA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP5PF ( NM_001320266.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307195.1 Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu27076
Clone ID Related Accession (Same CDS sequence)	XM_005260992.3 , NM_001003703.1 , NM_001003696.1 , NM_001320267.1 , NM_001320266.1 , NM_001003697.1 , NM_001685.4
Accession Version	NM_001320266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	ATP synthase-coupling factor 6, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC001178.1, HY064673.1, BQ268216.1, HY146471.1, AA364611.1 and AI085837.1. On Feb 18, 2016 this sequence version replaced XM_005260992.3. Transcript Variant: This variant (6) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, 4, 6 and 7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.63872.1, ERR279828.9182.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATTCTTC AGAGGCTCTT CAGGTTCTCC TCTGTCATTC GGTCAGCCGT CTCAGTCCAT 
TTGCGGAGGA ACATTGGTGT TACAGCAGTG GCATTTAATA AGGAACTTGA TCCTATACAG 
AAACTCTTTG TGGACAAGAT TAGAGAATAC AAATCTAAGC GACAGACATC TGGAGGACCT 
GTTGATGCTA GTTCAGAGTA TCAGCAAGAG CTGGAGAGGG AGCTTTTTAA GCTCAAGCAA 
ATGTTTGGTA ATGCAGACAT GAATACATTT CCCACCTTCA AATTTGAAGA TCCCAAATTT 
GAAGTCATCG AAAAACCCCA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307195.1
CDS	853..1179
Translation	MILQRLFRFSSVIRSAVSVHLRRNIGVTAVAFNKELDPIQKLFVDKIREYKSKRQTSGGPVDASSEYQQELERELFKLKQMFGNADMNTFPTFKFEDPKFEVIEKPQA

Target ORF information:

RefSeq Version	NM_001320266.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320266.1

ORF Insert Sequence:

ATGATTCTTC AGAGGCTCTT CAGGTTCTCC TCTGTCATTC GGTCAGCCGT CTCAGTCCAT 
TTGCGGAGGA ACATTGGTGT TACAGCAGTG GCATTTAATA AGGAACTTGA TCCTATACAG 
AAACTCTTTG TGGACAAGAT TAGAGAATAC AAATCTAAGC GACAGACATC TGGAGGACCT 
GTTGATGCTA GTTCAGAGTA TCAGCAAGAG CTGGAGAGGG AGCTTTTTAA GCTCAAGCAA 
ATGTTTGGTA ATGCAGACAT GAATACATTT CCCACCTTCA AATTTGAAGA TCCCAAATTT 
GAAGTCATCG AAAAACCCCA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP5PF ( NM_001003697.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001003697.1 Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu27076
Clone ID Related Accession (Same CDS sequence)	XM_005260992.3 , NM_001003703.1 , NM_001003696.1 , NM_001320267.1 , NM_001320266.1 , NM_001003697.1 , NM_001685.4
Accession Version	NM_001003697.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Homo sapiens(human)
Product	ATP synthase-coupling factor 6, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC001178.1, BG938438.1, BC066310.1 and AI085837.1. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, 4, 6 and 7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.52765.1, SRR1163655.462939.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATTCTTC AGAGGCTCTT CAGGTTCTCC TCTGTCATTC GGTCAGCCGT CTCAGTCCAT 
TTGCGGAGGA ACATTGGTGT TACAGCAGTG GCATTTAATA AGGAACTTGA TCCTATACAG 
AAACTCTTTG TGGACAAGAT TAGAGAATAC AAATCTAAGC GACAGACATC TGGAGGACCT 
GTTGATGCTA GTTCAGAGTA TCAGCAAGAG CTGGAGAGGG AGCTTTTTAA GCTCAAGCAA 
ATGTTTGGTA ATGCAGACAT GAATACATTT CCCACCTTCA AATTTGAAGA TCCCAAATTT 
GAAGTCATCG AAAAACCCCA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003697.1
CDS	176..502
Translation	MILQRLFRFSSVIRSAVSVHLRRNIGVTAVAFNKELDPIQKLFVDKIREYKSKRQTSGGPVDASSEYQQELERELFKLKQMFGNADMNTFPTFKFEDPKFEVIEKPQA

Target ORF information:

RefSeq Version	NM_001003697.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003697.1

ORF Insert Sequence:

ATGATTCTTC AGAGGCTCTT CAGGTTCTCC TCTGTCATTC GGTCAGCCGT CTCAGTCCAT 
TTGCGGAGGA ACATTGGTGT TACAGCAGTG GCATTTAATA AGGAACTTGA TCCTATACAG 
AAACTCTTTG TGGACAAGAT TAGAGAATAC AAATCTAAGC GACAGACATC TGGAGGACCT 
GTTGATGCTA GTTCAGAGTA TCAGCAAGAG CTGGAGAGGG AGCTTTTTAA GCTCAAGCAA 
ATGTTTGGTA ATGCAGACAT GAATACATTT CCCACCTTCA AATTTGAAGA TCCCAAATTT 
GAAGTCATCG AAAAACCCCA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP5PF ( NM_001685.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001676.2 Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 2, mRNA.

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CloneID	OHu27076
Clone ID Related Accession (Same CDS sequence)	XM_005260992.3 , NM_001003703.1 , NM_001003696.1 , NM_001320267.1 , NM_001320266.1 , NM_001003697.1 , NM_001685.4
Accession Version	NM_001685.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	ATP synthase-coupling factor 6, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC001178.1, BC066310.1 and AI085837.1. On Aug 20, 2004 this sequence version replaced NM_001685.3. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, 4, 6 and 7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279872.1517.1, SRR1163658.514135.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03568911, SAMN03568912 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATTCTTC AGAGGCTCTT CAGGTTCTCC TCTGTCATTC GGTCAGCCGT CTCAGTCCAT 
TTGCGGAGGA ACATTGGTGT TACAGCAGTG GCATTTAATA AGGAACTTGA TCCTATACAG 
AAACTCTTTG TGGACAAGAT TAGAGAATAC AAATCTAAGC GACAGACATC TGGAGGACCT 
GTTGATGCTA GTTCAGAGTA TCAGCAAGAG CTGGAGAGGG AGCTTTTTAA GCTCAAGCAA 
ATGTTTGGTA ATGCAGACAT GAATACATTT CCCACCTTCA AATTTGAAGA TCCCAAATTT 
GAAGTCATCG AAAAACCCCA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001676.2
CDS	674..1000
Translation	MILQRLFRFSSVIRSAVSVHLRRNIGVTAVAFNKELDPIQKLFVDKIREYKSKRQTSGGPVDASSEYQQELERELFKLKQMFGNADMNTFPTFKFEDPKFEVIEKPQA

Target ORF information:

RefSeq Version	NM_001685.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001685.4

ORF Insert Sequence:

ATGATTCTTC AGAGGCTCTT CAGGTTCTCC TCTGTCATTC GGTCAGCCGT CTCAGTCCAT 
TTGCGGAGGA ACATTGGTGT TACAGCAGTG GCATTTAATA AGGAACTTGA TCCTATACAG 
AAACTCTTTG TGGACAAGAT TAGAGAATAC AAATCTAAGC GACAGACATC TGGAGGACCT 
GTTGATGCTA GTTCAGAGTA TCAGCAAGAG CTGGAGAGGG AGCTTTTTAA GCTCAAGCAA 
ATGTTTGGTA ATGCAGACAT GAATACATTT CCCACCTTCA AATTTGAAGA TCCCAAATTT 
GAAGTCATCG AAAAACCCCA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP5PF ( XM_005260992.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005261049.1 Homo sapiens ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (ATP5J), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu27076
Clone ID Related Accession (Same CDS sequence)	XM_005260992.3 , NM_001003703.1 , NM_001003696.1 , NM_001320267.1 , NM_001320266.1 , NM_001003697.1 , NM_001685.4
Accession Version	XM_005260992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	ATP synthase-coupling factor 6, mitochondrial isoform X1
Comment

ATGATTCTTC AGAGGCTCTT CAGGTTCTCC TCTGTCATTC GGTCAGCCGT CTCAGTCCAT 
TTGCGGAGGA ACATTGGTGT TACAGCAGTG GCATTTAATA AGGAACTTGA TCCTATACAG 
AAACTCTTTG TGGACAAGAT TAGAGAATAC AAATCTAAGC GACAGACATC TGGAGGACCT 
GTTGATGCTA GTTCAGAGTA TCAGCAAGAG CTGGAGAGGG AGCTTTTTAA GCTCAAGCAA 
ATGTTTGGTA ATGCAGACAT GAATACATTT CCCACCTTCA AATTTGAAGA TCCCAAATTT 
GAAGTCATCG AAAAACCCCA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005261049.1
Translation	MILQRLFRFSSVIRSAVSVHLRRNIGVTAVAFNKELDPIQKLFVDKIREYKSKRQTSGGPVDASSEYQQELERELFKLKQMFGNADMNTFPTFKFEDPKFEVIEKPQA

Target ORF information:

RefSeq Version	XM_005260992.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (ATP5J), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005260992.3

ORF Insert Sequence:

ATGATTCTTC AGAGGCTCTT CAGGTTCTCC TCTGTCATTC GGTCAGCCGT CTCAGTCCAT 
TTGCGGAGGA ACATTGGTGT TACAGCAGTG GCATTTAATA AGGAACTTGA TCCTATACAG 
AAACTCTTTG TGGACAAGAT TAGAGAATAC AAATCTAAGC GACAGACATC TGGAGGACCT 
GTTGATGCTA GTTCAGAGTA TCAGCAAGAG CTGGAGAGGG AGCTTTTTAA GCTCAAGCAA 
ATGTTTGGTA ATGCAGACAT GAATACATTT CCCACCTTCA AATTTGAAGA TCCCAAATTT 
GAAGTCATCG AAAAACCCCA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP5PF ( NM_001003701.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001003701.1 Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu26650
Clone ID Related Accession (Same CDS sequence)	NM_001003701.1
Accession Version	NM_001003701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Homo sapiens(human)
Product	ATP synthase-coupling factor 6, mitochondrial isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC001178.1, BI093033.1, BC066310.1 and AI085837.1. Transcript Variant: This variant (5) differs in the 5' UTR and the 5' coding region, compared to variant 1. The predicted isoform (b) is longer, and it contains a distinct N-terminus, compared to isoform a. The translation initiation site for this transcript is inferred. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DT217787.1, BG563080.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155371 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCATTGCG ATGGCGGAAT CAGCATGATT CTTCAGAGGC TCTTCAGGTT CTCCTCTGTC 
ATTCGGTCAG CCGTCTCAGT CCATTTGCGG AGGAACATTG GTGTTACAGC AGTGGCATTT 
AATAAGGAAC TTGATCCTAT ACAGAAACTC TTTGTGGACA AGATTAGAGA ATACAAATCT 
AAGCGACAGA CATCTGGAGG ACCTGTTGAT GCTAGTTCAG AGTATCAGCA AGAGCTGGAG 
AGGGAGCTTT TTAAGCTCAA GCAAATGTTT GGTAATGCAG ACATGAATAC ATTTCCCACC 
TTCAAATTTG AAGATCCCAA ATTTGAAGTC ATCGAAAAAC CCCAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003701.1
CDS	700..1050
Translation	MHCDGGISMILQRLFRFSSVIRSAVSVHLRRNIGVTAVAFNKELDPIQKLFVDKIREYKSKRQTSGGPVDASSEYQQELERELFKLKQMFGNADMNTFPTFKFEDPKFEVIEKPQA

Target ORF information:

RefSeq Version	NM_001003701.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003701.1

ORF Insert Sequence:

ATGCATTGCG ATGGCGGAAT CAGCATGATT CTTCAGAGGC TCTTCAGGTT CTCCTCTGTC 
ATTCGGTCAG CCGTCTCAGT CCATTTGCGG AGGAACATTG GTGTTACAGC AGTGGCATTT 
AATAAGGAAC TTGATCCTAT ACAGAAACTC TTTGTGGACA AGATTAGAGA ATACAAATCT 
AAGCGACAGA CATCTGGAGG ACCTGTTGAT GCTAGTTCAG AGTATCAGCA AGAGCTGGAG 
AGGGAGCTTT TTAAGCTCAA GCAAATGTTT GGTAATGCAG ACATGAATAC ATTTCCCACC 
TTCAAATTTG AAGATCCCAA ATTTGAAGTC ATCGAAAAAC CCCAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.
Molecular cell63(4)621-632(2016 Aug)
Floyd BJ,Wilkerson EM,Veling MT,Minogue CE,Xia C,Beebe ET,Wrobel RL,Cho H,Kremer LS,Alston CL,Gromek KA,Dolan BK,Ulbrich A,Stefely JA,Bohl SL,Werner KM,Jochem A,Westphall MS,Rensvold JW,Taylor RW,Prokisch H,Kim JP,Coon JJ,Pagliarini DJ

Mitochondrial Inhibitory Factor Protein 1 Functions as an Endogenous Inhibitor for Coupling Factor 6.
Journal of cellular biochemistry117(7)1680-7(2016 07)
Kawai M,Osanai T,Tanaka M,Magota K,Tomita H,Okumura K

Over-expression of the ATP5J gene correlates with cell migration and 5-fluorouracil sensitivity in colorectal cancer.
PloS one8(10)e76846(2013)
Zhu H,Chen L,Zhou W,Huang Z,Hu J,Dai S,Wang X,Huang X,He C

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ATP5PF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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ORF Insert Sequence:

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