ENPP3 cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ENPP3
Entrez Gene ID	5169
Full Name	ectonucleotide pyrophosphatase/phosphodiesterase 3
Synonyms	B10,CD203c,NPP3,PD-IBETA,PDNP3
General protein information	Preferred Names ectonucleotide pyrophosphatase/phosphodiesterase 3 Names ectonucleotide pyrophosphatase/phosphodiesterase family member 3 dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) gp130RB13-6 phosphodiesterase I/nucleotide pyrophosphatase 3 phosphodiesterase-I beta
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 6 Map Location: 6q23.2
Summary	The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Oct 2015].
Disorder MIM:	602182

mRNA and Protein(s)

mRNA	Protein	Name
XM_011535897.1	XP_011534199.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 3 isoform X2
NM_005021.4	NP_005012.2	ectonucleotide pyrophosphatase/phosphodiesterase family member 3
NM_005021.5	NP_005012.2	ectonucleotide pyrophosphatase/phosphodiesterase family member 3
XM_017010932.1	XP_016866421.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 3 isoform X1
NM_005021.3	NP_005012.2	ectonucleotide pyrophosphatase/phosphodiesterase family member 3

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT4G29690	NP_194698.1
Canis lupus familiaris (dog)	ENPP3	XP_005627149.1
Mus musculus (house mouse)	Enpp3	NP_598766.2
	ENPP3	XP_001103528.1
Gallus gallus (chicken)	ENPP3	XP_003641087.1
Arabidopsis thaliana (thale cress)	AT4G29700	NP_194699.1
Xenopus tropicalis (tropical clawed frog)	enpp3	XP_002936359.2
Pan troglodytes (chimpanzee)	ENPP3	XP_518741.2
Homo sapiens (human)	ENPP3	NP_005012.2
Bos taurus (cattle)	ENPP3	NP_001069391.1
Rattus norvegicus (Norway rat)	Enpp3	NP_062243.2

Gene Ontology
Bibliography

Related articles in PubMed

Blood basophil activation is a reliable biomarker of allergic bronchopulmonary aspergillosis in cystic fibrosis.
Gernez Y, Walters J, Mirkovi? B, Lavelle GM, Colleen DE, Davies ZA, Everson C, Tirouvanziam R, Silver E, Wallenstein S, Chotirmall SH, McElvaney NG, Herzenberg LA, Moss RB
The European respiratory journal47(1)177-85(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Basophil CD203c surface expression reliably discriminated cystic fibrosis with allergic bronchopulmonary aspergillosis from cystic fibrosis with Aspergillus colonization and cystic fibrosis over time.
Title: Blood basophil activation is a reliable biomarker of allergic bronchopulmonary aspergillosis in cystic fibrosis.

Both NPP1 and NPP3 ectoenzymes are expressed in N2a cells, their levels dramatically changing when cells differentiate into a neuronal-like phenotype
Title: Ectonucleotide pyrophosphatase/phosphodiesterase activity in Neuro-2a neuroblastoma cells: changes in expression associated with neuronal differentiation.

Anaphylactic transfusion reaction in homozygous haptoglobin deficiency detected by CD203c expression on basophils.
Title: Anaphylactic transfusion reaction in homozygous haptoglobin deficiency detected by CD203c expression on basophils.

Expression of CD203c on basophils as a marker of immunoglobulin E-mediated (L)-asparaginase allergy.
Title: Expression of CD203c on basophils as a marker of immunoglobulin E-mediated (L)-asparaginase allergy.

ENPP3 is a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb)
Title: Identification of ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb).

The following ENPP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ENPP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19400	NM_005021.5 Latest version!	Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu59605	XM_011535897.1 Latest version!	Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu96648	XM_017010932.1 Latest version!	Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu19400	NM_005021.4	Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu19400	NM_005021.3	Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ENPP3 ( NM_005021.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_005012.2 Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19400
Clone ID Related Accession (Same CDS sequence)	NM_005021.4 , NM_005021.5 , NM_005021.3
Accession Version	NM_005021.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY106781.1, AL121575.24, EF560735.1 and AK024899.1. This sequence is a reference standard in the RefSeqGene project. On Oct 10, 2015 this sequence version replaced NM_005021.3. Transcript Variant: This variant (1) represents the longer, protein-coding transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK024899.1, AK292903.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAATCTA CGTTGACTTT AGCAACGGAA CAACCTGTTA AGAAGAACAC TCTTAAGAAA 
TATAAAATAG CTTGCATTGT TCTTCTTGCT TTGCTGGTGA TCATGTCACT TGGATTAGGC 
CTGGGGCTTG GACTCAGGAA ACTGGAAAAG CAAGGCAGCT GCAGGAAGAA GTGCTTTGAT 
GCATCATTTA GAGGACTGGA GAACTGCCGG TGTGATGTGG CATGTAAAGA CCGAGGTGAT 
TGCTGCTGGG ATTTTGAAGA CACCTGTGTG GAATCAACTC GAATATGGAT GTGCAATAAA 
TTTCGTTGTG GAGAGACCAG ATTAGAGGCC AGCCTTTGCT CTTGTTCAGA TGACTGTTTG 
CAGAGGAAAG ATTGCTGTGC TGACTATAAG AGTGTTTGCC AAGGAGAAAC CTCATGGCTG 
GAAGAAAACT GTGACACAGC CCAGCAGTCT CAGTGCCCAG AAGGGTTTGA CCTGCCACCA 
GTTATCTTGT TTTCTATGGA TGGATTTAGA GCTGAATATT TATACACATG GGATACTTTA 
ATGCCAAATA TCAATAAACT GAAAACATGT GGAATTCATT CAAAATACAT GAGAGCTATG 
TATCCTACCA AAACCTTCCC AAATCATTAC ACCATTGTCA CGGGCTTGTA TCCAGAGTCA 
CATGGCATCA TTGACAATAA TATGTATGAT GTAAATCTCA ACAAGAATTT TTCACTTTCT 
TCAAAGGAAC AAAATAATCC AGCCTGGTGG CATGGGCAAC CAATGTGGCT GACAGCAATG 
TATCAAGGTT TAAAAGCCGC TACCTACTTT TGGCCCGGAT CAGAAGTGGC TATAAATGGC 
TCCTTTCCTT CCATATACAT GCCTTACAAC GGAAGTGTCC CATTTGAAGA GAGGATTTCT 
ACACTGTTAA AATGGCTGGA CCTGCCCAAA GCTGAAAGAC CCAGGTTTTA TACCATGTAT 
TTTGAAGAAC CTGATTCCTC TGGACATGCA GGTGGACCAG TCAGTGCCAG AGTAATTAAA 
GCCTTACAGG TAGTAGATCA TGCTTTTGGG ATGTTGATGG AAGGCCTGAA GCAGCGGAAT 
TTGCACAACT GTGTCAATAT CATCCTTCTG GCTGACCATG GAATGGACCA GACTTATTGT 
AACAAGATGG AATACATGAC TGATTATTTT CCCAGAATAA ACTTCTTCTA CATGTACGAA 
GGGCCTGCCC CCCGCATCCG AGCTCATAAT ATACCTCATG ACTTTTTTAG TTTTAATTCT 
GAGGAAATTG TTAGAAACCT CAGTTGCCGA AAACCTGATC AGCATTTCAA GCCCTATTTG 
ACTCCTGATT TGCCAAAGCG ACTGCACTAT GCCAAGAACG TCAGAATCGA CAAAGTTCAT 
CTCTTTGTGG ATCAACAGTG GCTGGCTGTT AGGAGTAAAT CAAATACAAA TTGTGGAGGA 
GGCAACCATG GTTATAACAA TGAGTTTAGG AGCATGGAGG CTATCTTTCT GGCACATGGA 
CCCAGTTTTA AAGAGAAGAC TGAAGTTGAA CCATTTGAAA ATATTGAAGT CTATAACCTA 
ATGTGTGATC TTCTACGCAT TCAACCAGCA CCAAACAATG GAACCCATGG TAGTTTAAAC 
CATCTTCTGA AGGTGCCTTT TTATGAGCCA TCCCATGCAG AGGAGGTGTC AAAGTTTTCT 
GTTTGTGGCT TTGCTAATCC ATTGCCCACA GAGTCTCTTG ACTGTTTCTG CCCTCACCTA 
CAAAATAGTA CTCAGCTGGA ACAAGTGAAT CAGATGCTAA ATCTCACCCA AGAAGAAATA 
ACAGCAACAG TGAAAGTAAA TTTGCCATTT GGGAGGCCTA GGGTACTGCA GAAGAACGTG 
GACCACTGTC TCCTTTACCA CAGGGAATAT GTCAGTGGAT TTGGAAAAGC TATGAGGATG 
CCCATGTGGA GTTCATACAC AGTCCCCCAG TTGGGAGACA CATCGCCTCT GCCTCCCACT 
GTCCCAGACT GTCTGCGGGC TGATGTCAGG GTTCCTCCTT CTGAGAGCCA AAAATGTTCC 
TTCTATTTAG CAGACAAGAA TATCACCCAC GGCTTCCTCT ATCCTCCTGC CAGCAATAGA 
ACATCAGATA GCCAATATGA TGCTTTAATT ACTAGCAATT TGGTACCTAT GTATGAAGAA 
TTCAGAAAAA TGTGGGACTA CTTCCACAGT GTTCTTCTTA TAAAACATGC CACAGAAAGA 
AATGGAGTAA ATGTGGTTAG TGGACCAATA TTTGATTATA ATTATGATGG CCATTTTGAT 
GCTCCAGATG AAATTACCAA ACATTTAGCC AACACTGATG TTCCCATCCC AACACACTAC 
TTTGTGGTGC TGACCAGTTG TAAAAACAAG AGCCACACAC CGGAAAACTG CCCTGGGTGG 
CTGGATGTCC TACCCTTTAT CATCCCTCAC CGACCTACCA ACGTGGAGAG CTGTCCTGAA 
GGTAAACCAG AAGCTCTTTG GGTTGAAGAA AGATTTACAG CTCACATTGC CCGGGTCCGT 
GATGTAGAAC TTCTCACTGG GCTTGACTTC TATCAGGATA AAGTGCAGCC TGTCTCTGAA 
ATTTTGCAAC TAAAGACATA TTTACCAACA TTTGAAACCA CTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005012.2
CDS	151..2778
Translation	MESTLTLATEQPVKKNTLKKYKIACIVLLALLVIMSLGLGLGLGLRKLEKQGSCRKKCFDASFRGLENCRCDVACKDRGDCCWDFEDTCVESTRIWMCNKFRCGETRLEASLCSCSDDCLQRKDCCADYKSVCQGETSWLEENCDTAQQSQCPEGFDLPPVILFSMDGFRAEYLYTWDTLMPNINKLKTCGIHSKYMRAMYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVNLNKNFSLSSKEQNNPAWWHGQPMWLTAMYQGLKAATYFWPGSEVAINGSFPSIYMPYNGSVPFEERISTLLKWLDLPKAERPRFYTMYFEEPDSSGHAGGPVSARVIKALQVVDHAFGMLMEGLKQRNLHNCVNIILLADHGMDQTYCNKMEYMTDYFPRINFFYMYEGPAPRIRAHNIPHDFFSFNSEEIVRNLSCRKPDQHFKPYLTPDLPKRLHYAKNVRIDKVHLFVDQQWLAVRSKSNTNCGGGNHGYNNEFRSMEAIFLAHGPSFKEKTEVEPFENIEVYNLMCDLLRIQPAPNNGTHGSLNHLLKVPFYEPSHAEEVSKFSVCGFANPLPTESLDCFCPHLQNSTQLEQVNQMLNLTQEEITATVKVNLPFGRPRVLQKNVDHCLLYHREYVSGFGKAMRMPMWSSYTVPQLGDTSPLPPTVPDCLRADVRVPPSESQKCSFYLADKNITHGFLYPPASNRTSDSQYDALITSNLVPMYEEFRKMWDYFHSVLLIKHATERNGVNVVSGPIFDYNYDGHFDAPDEITKHLANTDVPIPTHYFVVLTSCKNKSHTPENCPGWLDVLPFIIPHRPTNVESCPEGKPEALWVEERFTAHIARVRDVELLTGLDFYQDKVQPVSEILQLKTYLPTFETTI

Target ORF information:

RefSeq Version	NM_005021.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005021.4

ORF Insert Sequence:

ATGGAATCTA CGTTGACTTT AGCAACGGAA CAACCTGTTA AGAAGAACAC TCTTAAGAAA 
TATAAAATAG CTTGCATTGT TCTTCTTGCT TTGCTGGTGA TCATGTCACT TGGATTAGGC 
CTGGGGCTTG GACTCAGGAA ACTGGAAAAG CAAGGCAGCT GCAGGAAGAA GTGCTTTGAT 
GCATCATTTA GAGGACTGGA GAACTGCCGG TGTGATGTGG CATGTAAAGA CCGAGGTGAT 
TGCTGCTGGG ATTTTGAAGA CACCTGTGTG GAATCAACTC GAATATGGAT GTGCAATAAA 
TTTCGTTGTG GAGAGACCAG ATTAGAGGCC AGCCTTTGCT CTTGTTCAGA TGACTGTTTG 
CAGAGGAAAG ATTGCTGTGC TGACTATAAG AGTGTTTGCC AAGGAGAAAC CTCATGGCTG 
GAAGAAAACT GTGACACAGC CCAGCAGTCT CAGTGCCCAG AAGGGTTTGA CCTGCCACCA 
GTTATCTTGT TTTCTATGGA TGGATTTAGA GCTGAATATT TATACACATG GGATACTTTA 
ATGCCAAATA TCAATAAACT GAAAACATGT GGAATTCATT CAAAATACAT GAGAGCTATG 
TATCCTACCA AAACCTTCCC AAATCATTAC ACCATTGTCA CGGGCTTGTA TCCAGAGTCA 
CATGGCATCA TTGACAATAA TATGTATGAT GTAAATCTCA ACAAGAATTT TTCACTTTCT 
TCAAAGGAAC AAAATAATCC AGCCTGGTGG CATGGGCAAC CAATGTGGCT GACAGCAATG 
TATCAAGGTT TAAAAGCCGC TACCTACTTT TGGCCCGGAT CAGAAGTGGC TATAAATGGC 
TCCTTTCCTT CCATATACAT GCCTTACAAC GGAAGTGTCC CATTTGAAGA GAGGATTTCT 
ACACTGTTAA AATGGCTGGA CCTGCCCAAA GCTGAAAGAC CCAGGTTTTA TACCATGTAT 
TTTGAAGAAC CTGATTCCTC TGGACATGCA GGTGGACCAG TCAGTGCCAG AGTAATTAAA 
GCCTTACAGG TAGTAGATCA TGCTTTTGGG ATGTTGATGG AAGGCCTGAA GCAGCGGAAT 
TTGCACAACT GTGTCAATAT CATCCTTCTG GCTGACCATG GAATGGACCA GACTTATTGT 
AACAAGATGG AATACATGAC TGATTATTTT CCCAGAATAA ACTTCTTCTA CATGTACGAA 
GGGCCTGCCC CCCGCATCCG AGCTCATAAT ATACCTCATG ACTTTTTTAG TTTTAATTCT 
GAGGAAATTG TTAGAAACCT CAGTTGCCGA AAACCTGATC AGCATTTCAA GCCCTATTTG 
ACTCCTGATT TGCCAAAGCG ACTGCACTAT GCCAAGAACG TCAGAATCGA CAAAGTTCAT 
CTCTTTGTGG ATCAACAGTG GCTGGCTGTT AGGAGTAAAT CAAATACAAA TTGTGGAGGA 
GGCAACCATG GTTATAACAA TGAGTTTAGG AGCATGGAGG CTATCTTTCT GGCACATGGA 
CCCAGTTTTA AAGAGAAGAC TGAAGTTGAA CCATTTGAAA ATATTGAAGT CTATAACCTA 
ATGTGTGATC TTCTACGCAT TCAACCAGCA CCAAACAATG GAACCCATGG TAGTTTAAAC 
CATCTTCTGA AGGTGCCTTT TTATGAGCCA TCCCATGCAG AGGAGGTGTC AAAGTTTTCT 
GTTTGTGGCT TTGCTAATCC ATTGCCCACA GAGTCTCTTG ACTGTTTCTG CCCTCACCTA 
CAAAATAGTA CTCAGCTGGA ACAAGTGAAT CAGATGCTAA ATCTCACCCA AGAAGAAATA 
ACAGCAACAG TGAAAGTAAA TTTGCCATTT GGGAGGCCTA GGGTACTGCA GAAGAACGTG 
GACCACTGTC TCCTTTACCA CAGGGAATAT GTCAGTGGAT TTGGAAAAGC TATGAGGATG 
CCCATGTGGA GTTCATACAC AGTCCCCCAG TTGGGAGACA CATCGCCTCT GCCTCCCACT 
GTCCCAGACT GTCTGCGGGC TGATGTCAGG GTTCCTCCTT CTGAGAGCCA AAAATGTTCC 
TTCTATTTAG CAGACAAGAA TATCACCCAC GGCTTCCTCT ATCCTCCTGC CAGCAATAGA 
ACATCAGATA GCCAATATGA TGCTTTAATT ACTAGCAATT TGGTACCTAT GTATGAAGAA 
TTCAGAAAAA TGTGGGACTA CTTCCACAGT GTTCTTCTTA TAAAACATGC CACAGAAAGA 
AATGGAGTAA ATGTGGTTAG TGGACCAATA TTTGATTATA ATTATGATGG CCATTTTGAT 
GCTCCAGATG AAATTACCAA ACATTTAGCC AACACTGATG TTCCCATCCC AACACACTAC 
TTTGTGGTGC TGACCAGTTG TAAAAACAAG AGCCACACAC CGGAAAACTG CCCTGGGTGG 
CTGGATGTCC TACCCTTTAT CATCCCTCAC CGACCTACCA ACGTGGAGAG CTGTCCTGAA 
GGTAAACCAG AAGCTCTTTG GGTTGAAGAA AGATTTACAG CTCACATTGC CCGGGTCCGT 
GATGTAGAAC TTCTCACTGG GCTTGACTTC TATCAGGATA AAGTGCAGCC TGTCTCTGAA 
ATTTTGCAAC TAAAGACATA TTTACCAACA TTTGAAACCA CTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENPP3 ( NM_005021.5 ) cDNA ORF clone, Homo sapiens(Human) -> NP_005012.2 Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19400
Clone ID Related Accession (Same CDS sequence)	NM_005021.4 , NM_005021.5 , NM_005021.3
Accession Version	NM_005021.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY106781.1, AL121575.24, EF560735.1 and AK024899.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005021.4. Transcript Variant: This variant (1) represents the longer, protein-coding transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK024899.1, AK292903.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000357639.8/ ENSP00000350265.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAATCTA CGTTGACTTT AGCAACGGAA CAACCTGTTA AGAAGAACAC TCTTAAGAAA 
TATAAAATAG CTTGCATTGT TCTTCTTGCT TTGCTGGTGA TCATGTCACT TGGATTAGGC 
CTGGGGCTTG GACTCAGGAA ACTGGAAAAG CAAGGCAGCT GCAGGAAGAA GTGCTTTGAT 
GCATCATTTA GAGGACTGGA GAACTGCCGG TGTGATGTGG CATGTAAAGA CCGAGGTGAT 
TGCTGCTGGG ATTTTGAAGA CACCTGTGTG GAATCAACTC GAATATGGAT GTGCAATAAA 
TTTCGTTGTG GAGAGACCAG ATTAGAGGCC AGCCTTTGCT CTTGTTCAGA TGACTGTTTG 
CAGAGGAAAG ATTGCTGTGC TGACTATAAG AGTGTTTGCC AAGGAGAAAC CTCATGGCTG 
GAAGAAAACT GTGACACAGC CCAGCAGTCT CAGTGCCCAG AAGGGTTTGA CCTGCCACCA 
GTTATCTTGT TTTCTATGGA TGGATTTAGA GCTGAATATT TATACACATG GGATACTTTA 
ATGCCAAATA TCAATAAACT GAAAACATGT GGAATTCATT CAAAATACAT GAGAGCTATG 
TATCCTACCA AAACCTTCCC AAATCATTAC ACCATTGTCA CGGGCTTGTA TCCAGAGTCA 
CATGGCATCA TTGACAATAA TATGTATGAT GTAAATCTCA ACAAGAATTT TTCACTTTCT 
TCAAAGGAAC AAAATAATCC AGCCTGGTGG CATGGGCAAC CAATGTGGCT GACAGCAATG 
TATCAAGGTT TAAAAGCCGC TACCTACTTT TGGCCCGGAT CAGAAGTGGC TATAAATGGC 
TCCTTTCCTT CCATATACAT GCCTTACAAC GGAAGTGTCC CATTTGAAGA GAGGATTTCT 
ACACTGTTAA AATGGCTGGA CCTGCCCAAA GCTGAAAGAC CCAGGTTTTA TACCATGTAT 
TTTGAAGAAC CTGATTCCTC TGGACATGCA GGTGGACCAG TCAGTGCCAG AGTAATTAAA 
GCCTTACAGG TAGTAGATCA TGCTTTTGGG ATGTTGATGG AAGGCCTGAA GCAGCGGAAT 
TTGCACAACT GTGTCAATAT CATCCTTCTG GCTGACCATG GAATGGACCA GACTTATTGT 
AACAAGATGG AATACATGAC TGATTATTTT CCCAGAATAA ACTTCTTCTA CATGTACGAA 
GGGCCTGCCC CCCGCATCCG AGCTCATAAT ATACCTCATG ACTTTTTTAG TTTTAATTCT 
GAGGAAATTG TTAGAAACCT CAGTTGCCGA AAACCTGATC AGCATTTCAA GCCCTATTTG 
ACTCCTGATT TGCCAAAGCG ACTGCACTAT GCCAAGAACG TCAGAATCGA CAAAGTTCAT 
CTCTTTGTGG ATCAACAGTG GCTGGCTGTT AGGAGTAAAT CAAATACAAA TTGTGGAGGA 
GGCAACCATG GTTATAACAA TGAGTTTAGG AGCATGGAGG CTATCTTTCT GGCACATGGA 
CCCAGTTTTA AAGAGAAGAC TGAAGTTGAA CCATTTGAAA ATATTGAAGT CTATAACCTA 
ATGTGTGATC TTCTACGCAT TCAACCAGCA CCAAACAATG GAACCCATGG TAGTTTAAAC 
CATCTTCTGA AGGTGCCTTT TTATGAGCCA TCCCATGCAG AGGAGGTGTC AAAGTTTTCT 
GTTTGTGGCT TTGCTAATCC ATTGCCCACA GAGTCTCTTG ACTGTTTCTG CCCTCACCTA 
CAAAATAGTA CTCAGCTGGA ACAAGTGAAT CAGATGCTAA ATCTCACCCA AGAAGAAATA 
ACAGCAACAG TGAAAGTAAA TTTGCCATTT GGGAGGCCTA GGGTACTGCA GAAGAACGTG 
GACCACTGTC TCCTTTACCA CAGGGAATAT GTCAGTGGAT TTGGAAAAGC TATGAGGATG 
CCCATGTGGA GTTCATACAC AGTCCCCCAG TTGGGAGACA CATCGCCTCT GCCTCCCACT 
GTCCCAGACT GTCTGCGGGC TGATGTCAGG GTTCCTCCTT CTGAGAGCCA AAAATGTTCC 
TTCTATTTAG CAGACAAGAA TATCACCCAC GGCTTCCTCT ATCCTCCTGC CAGCAATAGA 
ACATCAGATA GCCAATATGA TGCTTTAATT ACTAGCAATT TGGTACCTAT GTATGAAGAA 
TTCAGAAAAA TGTGGGACTA CTTCCACAGT GTTCTTCTTA TAAAACATGC CACAGAAAGA 
AATGGAGTAA ATGTGGTTAG TGGACCAATA TTTGATTATA ATTATGATGG CCATTTTGAT 
GCTCCAGATG AAATTACCAA ACATTTAGCC AACACTGATG TTCCCATCCC AACACACTAC 
TTTGTGGTGC TGACCAGTTG TAAAAACAAG AGCCACACAC CGGAAAACTG CCCTGGGTGG 
CTGGATGTCC TACCCTTTAT CATCCCTCAC CGACCTACCA ACGTGGAGAG CTGTCCTGAA 
GGTAAACCAG AAGCTCTTTG GGTTGAAGAA AGATTTACAG CTCACATTGC CCGGGTCCGT 
GATGTAGAAC TTCTCACTGG GCTTGACTTC TATCAGGATA AAGTGCAGCC TGTCTCTGAA 
ATTTTGCAAC TAAAGACATA TTTACCAACA TTTGAAACCA CTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005012.2
CDS	84..2711
Translation	MESTLTLATEQPVKKNTLKKYKIACIVLLALLVIMSLGLGLGLGLRKLEKQGSCRKKCFDASFRGLENCRCDVACKDRGDCCWDFEDTCVESTRIWMCNKFRCGETRLEASLCSCSDDCLQRKDCCADYKSVCQGETSWLEENCDTAQQSQCPEGFDLPPVILFSMDGFRAEYLYTWDTLMPNINKLKTCGIHSKYMRAMYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVNLNKNFSLSSKEQNNPAWWHGQPMWLTAMYQGLKAATYFWPGSEVAINGSFPSIYMPYNGSVPFEERISTLLKWLDLPKAERPRFYTMYFEEPDSSGHAGGPVSARVIKALQVVDHAFGMLMEGLKQRNLHNCVNIILLADHGMDQTYCNKMEYMTDYFPRINFFYMYEGPAPRIRAHNIPHDFFSFNSEEIVRNLSCRKPDQHFKPYLTPDLPKRLHYAKNVRIDKVHLFVDQQWLAVRSKSNTNCGGGNHGYNNEFRSMEAIFLAHGPSFKEKTEVEPFENIEVYNLMCDLLRIQPAPNNGTHGSLNHLLKVPFYEPSHAEEVSKFSVCGFANPLPTESLDCFCPHLQNSTQLEQVNQMLNLTQEEITATVKVNLPFGRPRVLQKNVDHCLLYHREYVSGFGKAMRMPMWSSYTVPQLGDTSPLPPTVPDCLRADVRVPPSESQKCSFYLADKNITHGFLYPPASNRTSDSQYDALITSNLVPMYEEFRKMWDYFHSVLLIKHATERNGVNVVSGPIFDYNYDGHFDAPDEITKHLANTDVPIPTHYFVVLTSCKNKSHTPENCPGWLDVLPFIIPHRPTNVESCPEGKPEALWVEERFTAHIARVRDVELLTGLDFYQDKVQPVSEILQLKTYLPTFETTI

Target ORF information:

RefSeq Version	NM_005021.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005021.5

ORF Insert Sequence:

ATGGAATCTA CGTTGACTTT AGCAACGGAA CAACCTGTTA AGAAGAACAC TCTTAAGAAA 
TATAAAATAG CTTGCATTGT TCTTCTTGCT TTGCTGGTGA TCATGTCACT TGGATTAGGC 
CTGGGGCTTG GACTCAGGAA ACTGGAAAAG CAAGGCAGCT GCAGGAAGAA GTGCTTTGAT 
GCATCATTTA GAGGACTGGA GAACTGCCGG TGTGATGTGG CATGTAAAGA CCGAGGTGAT 
TGCTGCTGGG ATTTTGAAGA CACCTGTGTG GAATCAACTC GAATATGGAT GTGCAATAAA 
TTTCGTTGTG GAGAGACCAG ATTAGAGGCC AGCCTTTGCT CTTGTTCAGA TGACTGTTTG 
CAGAGGAAAG ATTGCTGTGC TGACTATAAG AGTGTTTGCC AAGGAGAAAC CTCATGGCTG 
GAAGAAAACT GTGACACAGC CCAGCAGTCT CAGTGCCCAG AAGGGTTTGA CCTGCCACCA 
GTTATCTTGT TTTCTATGGA TGGATTTAGA GCTGAATATT TATACACATG GGATACTTTA 
ATGCCAAATA TCAATAAACT GAAAACATGT GGAATTCATT CAAAATACAT GAGAGCTATG 
TATCCTACCA AAACCTTCCC AAATCATTAC ACCATTGTCA CGGGCTTGTA TCCAGAGTCA 
CATGGCATCA TTGACAATAA TATGTATGAT GTAAATCTCA ACAAGAATTT TTCACTTTCT 
TCAAAGGAAC AAAATAATCC AGCCTGGTGG CATGGGCAAC CAATGTGGCT GACAGCAATG 
TATCAAGGTT TAAAAGCCGC TACCTACTTT TGGCCCGGAT CAGAAGTGGC TATAAATGGC 
TCCTTTCCTT CCATATACAT GCCTTACAAC GGAAGTGTCC CATTTGAAGA GAGGATTTCT 
ACACTGTTAA AATGGCTGGA CCTGCCCAAA GCTGAAAGAC CCAGGTTTTA TACCATGTAT 
TTTGAAGAAC CTGATTCCTC TGGACATGCA GGTGGACCAG TCAGTGCCAG AGTAATTAAA 
GCCTTACAGG TAGTAGATCA TGCTTTTGGG ATGTTGATGG AAGGCCTGAA GCAGCGGAAT 
TTGCACAACT GTGTCAATAT CATCCTTCTG GCTGACCATG GAATGGACCA GACTTATTGT 
AACAAGATGG AATACATGAC TGATTATTTT CCCAGAATAA ACTTCTTCTA CATGTACGAA 
GGGCCTGCCC CCCGCATCCG AGCTCATAAT ATACCTCATG ACTTTTTTAG TTTTAATTCT 
GAGGAAATTG TTAGAAACCT CAGTTGCCGA AAACCTGATC AGCATTTCAA GCCCTATTTG 
ACTCCTGATT TGCCAAAGCG ACTGCACTAT GCCAAGAACG TCAGAATCGA CAAAGTTCAT 
CTCTTTGTGG ATCAACAGTG GCTGGCTGTT AGGAGTAAAT CAAATACAAA TTGTGGAGGA 
GGCAACCATG GTTATAACAA TGAGTTTAGG AGCATGGAGG CTATCTTTCT GGCACATGGA 
CCCAGTTTTA AAGAGAAGAC TGAAGTTGAA CCATTTGAAA ATATTGAAGT CTATAACCTA 
ATGTGTGATC TTCTACGCAT TCAACCAGCA CCAAACAATG GAACCCATGG TAGTTTAAAC 
CATCTTCTGA AGGTGCCTTT TTATGAGCCA TCCCATGCAG AGGAGGTGTC AAAGTTTTCT 
GTTTGTGGCT TTGCTAATCC ATTGCCCACA GAGTCTCTTG ACTGTTTCTG CCCTCACCTA 
CAAAATAGTA CTCAGCTGGA ACAAGTGAAT CAGATGCTAA ATCTCACCCA AGAAGAAATA 
ACAGCAACAG TGAAAGTAAA TTTGCCATTT GGGAGGCCTA GGGTACTGCA GAAGAACGTG 
GACCACTGTC TCCTTTACCA CAGGGAATAT GTCAGTGGAT TTGGAAAAGC TATGAGGATG 
CCCATGTGGA GTTCATACAC AGTCCCCCAG TTGGGAGACA CATCGCCTCT GCCTCCCACT 
GTCCCAGACT GTCTGCGGGC TGATGTCAGG GTTCCTCCTT CTGAGAGCCA AAAATGTTCC 
TTCTATTTAG CAGACAAGAA TATCACCCAC GGCTTCCTCT ATCCTCCTGC CAGCAATAGA 
ACATCAGATA GCCAATATGA TGCTTTAATT ACTAGCAATT TGGTACCTAT GTATGAAGAA 
TTCAGAAAAA TGTGGGACTA CTTCCACAGT GTTCTTCTTA TAAAACATGC CACAGAAAGA 
AATGGAGTAA ATGTGGTTAG TGGACCAATA TTTGATTATA ATTATGATGG CCATTTTGAT 
GCTCCAGATG AAATTACCAA ACATTTAGCC AACACTGATG TTCCCATCCC AACACACTAC 
TTTGTGGTGC TGACCAGTTG TAAAAACAAG AGCCACACAC CGGAAAACTG CCCTGGGTGG 
CTGGATGTCC TACCCTTTAT CATCCCTCAC CGACCTACCA ACGTGGAGAG CTGTCCTGAA 
GGTAAACCAG AAGCTCTTTG GGTTGAAGAA AGATTTACAG CTCACATTGC CCGGGTCCGT 
GATGTAGAAC TTCTCACTGG GCTTGACTTC TATCAGGATA AAGTGCAGCC TGTCTCTGAA 
ATTTTGCAAC TAAAGACATA TTTACCAACA TTTGAAACCA CTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENPP3 ( NM_005021.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_005012.2 Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), mRNA.

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CloneID	OHu19400
Clone ID Related Accession (Same CDS sequence)	NM_005021.4 , NM_005021.5 , NM_005021.3
Accession Version	NM_005021.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 3
Comment

ATGGAATCTA CGTTGACTTT AGCAACGGAA CAACCTGTTA AGAAGAACAC TCTTAAGAAA 
TATAAAATAG CTTGCATTGT TCTTCTTGCT TTGCTGGTGA TCATGTCACT TGGATTAGGC 
CTGGGGCTTG GACTCAGGAA ACTGGAAAAG CAAGGCAGCT GCAGGAAGAA GTGCTTTGAT 
GCATCATTTA GAGGACTGGA GAACTGCCGG TGTGATGTGG CATGTAAAGA CCGAGGTGAT 
TGCTGCTGGG ATTTTGAAGA CACCTGTGTG GAATCAACTC GAATATGGAT GTGCAATAAA 
TTTCGTTGTG GAGAGACCAG ATTAGAGGCC AGCCTTTGCT CTTGTTCAGA TGACTGTTTG 
CAGAGGAAAG ATTGCTGTGC TGACTATAAG AGTGTTTGCC AAGGAGAAAC CTCATGGCTG 
GAAGAAAACT GTGACACAGC CCAGCAGTCT CAGTGCCCAG AAGGGTTTGA CCTGCCACCA 
GTTATCTTGT TTTCTATGGA TGGATTTAGA GCTGAATATT TATACACATG GGATACTTTA 
ATGCCAAATA TCAATAAACT GAAAACATGT GGAATTCATT CAAAATACAT GAGAGCTATG 
TATCCTACCA AAACCTTCCC AAATCATTAC ACCATTGTCA CGGGCTTGTA TCCAGAGTCA 
CATGGCATCA TTGACAATAA TATGTATGAT GTAAATCTCA ACAAGAATTT TTCACTTTCT 
TCAAAGGAAC AAAATAATCC AGCCTGGTGG CATGGGCAAC CAATGTGGCT GACAGCAATG 
TATCAAGGTT TAAAAGCCGC TACCTACTTT TGGCCCGGAT CAGAAGTGGC TATAAATGGC 
TCCTTTCCTT CCATATACAT GCCTTACAAC GGAAGTGTCC CATTTGAAGA GAGGATTTCT 
ACACTGTTAA AATGGCTGGA CCTGCCCAAA GCTGAAAGAC CCAGGTTTTA TACCATGTAT 
TTTGAAGAAC CTGATTCCTC TGGACATGCA GGTGGACCAG TCAGTGCCAG AGTAATTAAA 
GCCTTACAGG TAGTAGATCA TGCTTTTGGG ATGTTGATGG AAGGCCTGAA GCAGCGGAAT 
TTGCACAACT GTGTCAATAT CATCCTTCTG GCTGACCATG GAATGGACCA GACTTATTGT 
AACAAGATGG AATACATGAC TGATTATTTT CCCAGAATAA ACTTCTTCTA CATGTACGAA 
GGGCCTGCCC CCCGCATCCG AGCTCATAAT ATACCTCATG ACTTTTTTAG TTTTAATTCT 
GAGGAAATTG TTAGAAACCT CAGTTGCCGA AAACCTGATC AGCATTTCAA GCCCTATTTG 
ACTCCTGATT TGCCAAAGCG ACTGCACTAT GCCAAGAACG TCAGAATCGA CAAAGTTCAT 
CTCTTTGTGG ATCAACAGTG GCTGGCTGTT AGGAGTAAAT CAAATACAAA TTGTGGAGGA 
GGCAACCATG GTTATAACAA TGAGTTTAGG AGCATGGAGG CTATCTTTCT GGCACATGGA 
CCCAGTTTTA AAGAGAAGAC TGAAGTTGAA CCATTTGAAA ATATTGAAGT CTATAACCTA 
ATGTGTGATC TTCTACGCAT TCAACCAGCA CCAAACAATG GAACCCATGG TAGTTTAAAC 
CATCTTCTGA AGGTGCCTTT TTATGAGCCA TCCCATGCAG AGGAGGTGTC AAAGTTTTCT 
GTTTGTGGCT TTGCTAATCC ATTGCCCACA GAGTCTCTTG ACTGTTTCTG CCCTCACCTA 
CAAAATAGTA CTCAGCTGGA ACAAGTGAAT CAGATGCTAA ATCTCACCCA AGAAGAAATA 
ACAGCAACAG TGAAAGTAAA TTTGCCATTT GGGAGGCCTA GGGTACTGCA GAAGAACGTG 
GACCACTGTC TCCTTTACCA CAGGGAATAT GTCAGTGGAT TTGGAAAAGC TATGAGGATG 
CCCATGTGGA GTTCATACAC AGTCCCCCAG TTGGGAGACA CATCGCCTCT GCCTCCCACT 
GTCCCAGACT GTCTGCGGGC TGATGTCAGG GTTCCTCCTT CTGAGAGCCA AAAATGTTCC 
TTCTATTTAG CAGACAAGAA TATCACCCAC GGCTTCCTCT ATCCTCCTGC CAGCAATAGA 
ACATCAGATA GCCAATATGA TGCTTTAATT ACTAGCAATT TGGTACCTAT GTATGAAGAA 
TTCAGAAAAA TGTGGGACTA CTTCCACAGT GTTCTTCTTA TAAAACATGC CACAGAAAGA 
AATGGAGTAA ATGTGGTTAG TGGACCAATA TTTGATTATA ATTATGATGG CCATTTTGAT 
GCTCCAGATG AAATTACCAA ACATTTAGCC AACACTGATG TTCCCATCCC AACACACTAC 
TTTGTGGTGC TGACCAGTTG TAAAAACAAG AGCCACACAC CGGAAAACTG CCCTGGGTGG 
CTGGATGTCC TACCCTTTAT CATCCCTCAC CGACCTACCA ACGTGGAGAG CTGTCCTGAA 
GGTAAACCAG AAGCTCTTTG GGTTGAAGAA AGATTTACAG CTCACATTGC CCGGGTCCGT 
GATGTAGAAC TTCTCACTGG GCTTGACTTC TATCAGGATA AAGTGCAGCC TGTCTCTGAA 
ATTTTGCAAC TAAAGACATA TTTACCAACA TTTGAAACCA CTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_005012.2

Translation

MESTLTLATEQPVKKNTLKKYKIACIVLLALLVIMSLGLGLGLGLRKLEKQGSCRKKCFDASFRGLENCRCDVACKDRGDCCWDFEDTCVESTRIWMCNKFRCGETRLEASLCSCSDDCLQRKDCCADYKSVCQGETSWLEENCDTAQQSQCPEGFDLPPVILFSMDGFRAEYLYTWDTLMPNINKLKTCGIHSKYMRAMYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVNLNKNFSLSSKEQNNPAWWHGQPMWLTAMYQGLKAATYFWPGSEVAINGSFPSIYMPYNGSVPFEERISTLLKWLDLPKAERPRFYTMYFEEPDSSGHAGGPVSARVIKALQVVDHAFGMLMEGLKQRNLHNCVNIILLADHGMDQTYCNKMEYMTDYFPRINFFYMYEGPAPRIRAHNIPHDFFSFNSEEIVRNLSCRKPDQHFKPYLTPDLPKRLHYAKNVRIDKVHLFVDQQWLAVRSKSNTNCGGGNHGYNNEFRSMEAIFLAHGPSFKEKTEVEPFENIEVYNLMCDLLRIQPAPNNGTHGSLNHLLKVPFYEPSHAEEVSKFSVCGFANPLPTESLDCFCPHLQNSTQLEQVNQMLNLTQEEITATVKVNLPFGRPRVLQKNVDHCLLYHREYVSGFGKAMRMPMWSSYTVPQLGDTSPLPPTVPDCLRADVRVPPSESQKCSFYLADKNITHGFLYPPASNRTSDSQYDALITSNLVPMYEEFRKMWDYFHSVLLIKHATERNGVNVVSGPIFDYNYDGHFDAPDEITKHLANTDVPIPTHYFVVLTSCKNKSHTPENCPGWLDVLPFIIPHRPTNVESCPEGKPEALWVEERFTAHIARVRDVELLTGLDFYQDKVQPVSEILQLKTYLPTFETTI

Target ORF information:

RefSeq Version	NM_005021.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005021.3

ORF Insert Sequence:

ATGGAATCTA CGTTGACTTT AGCAACGGAA CAACCTGTTA AGAAGAACAC TCTTAAGAAA 
TATAAAATAG CTTGCATTGT TCTTCTTGCT TTGCTGGTGA TCATGTCACT TGGATTAGGC 
CTGGGGCTTG GACTCAGGAA ACTGGAAAAG CAAGGCAGCT GCAGGAAGAA GTGCTTTGAT 
GCATCATTTA GAGGACTGGA GAACTGCCGG TGTGATGTGG CATGTAAAGA CCGAGGTGAT 
TGCTGCTGGG ATTTTGAAGA CACCTGTGTG GAATCAACTC GAATATGGAT GTGCAATAAA 
TTTCGTTGTG GAGAGACCAG ATTAGAGGCC AGCCTTTGCT CTTGTTCAGA TGACTGTTTG 
CAGAGGAAAG ATTGCTGTGC TGACTATAAG AGTGTTTGCC AAGGAGAAAC CTCATGGCTG 
GAAGAAAACT GTGACACAGC CCAGCAGTCT CAGTGCCCAG AAGGGTTTGA CCTGCCACCA 
GTTATCTTGT TTTCTATGGA TGGATTTAGA GCTGAATATT TATACACATG GGATACTTTA 
ATGCCAAATA TCAATAAACT GAAAACATGT GGAATTCATT CAAAATACAT GAGAGCTATG 
TATCCTACCA AAACCTTCCC AAATCATTAC ACCATTGTCA CGGGCTTGTA TCCAGAGTCA 
CATGGCATCA TTGACAATAA TATGTATGAT GTAAATCTCA ACAAGAATTT TTCACTTTCT 
TCAAAGGAAC AAAATAATCC AGCCTGGTGG CATGGGCAAC CAATGTGGCT GACAGCAATG 
TATCAAGGTT TAAAAGCCGC TACCTACTTT TGGCCCGGAT CAGAAGTGGC TATAAATGGC 
TCCTTTCCTT CCATATACAT GCCTTACAAC GGAAGTGTCC CATTTGAAGA GAGGATTTCT 
ACACTGTTAA AATGGCTGGA CCTGCCCAAA GCTGAAAGAC CCAGGTTTTA TACCATGTAT 
TTTGAAGAAC CTGATTCCTC TGGACATGCA GGTGGACCAG TCAGTGCCAG AGTAATTAAA 
GCCTTACAGG TAGTAGATCA TGCTTTTGGG ATGTTGATGG AAGGCCTGAA GCAGCGGAAT 
TTGCACAACT GTGTCAATAT CATCCTTCTG GCTGACCATG GAATGGACCA GACTTATTGT 
AACAAGATGG AATACATGAC TGATTATTTT CCCAGAATAA ACTTCTTCTA CATGTACGAA 
GGGCCTGCCC CCCGCATCCG AGCTCATAAT ATACCTCATG ACTTTTTTAG TTTTAATTCT 
GAGGAAATTG TTAGAAACCT CAGTTGCCGA AAACCTGATC AGCATTTCAA GCCCTATTTG 
ACTCCTGATT TGCCAAAGCG ACTGCACTAT GCCAAGAACG TCAGAATCGA CAAAGTTCAT 
CTCTTTGTGG ATCAACAGTG GCTGGCTGTT AGGAGTAAAT CAAATACAAA TTGTGGAGGA 
GGCAACCATG GTTATAACAA TGAGTTTAGG AGCATGGAGG CTATCTTTCT GGCACATGGA 
CCCAGTTTTA AAGAGAAGAC TGAAGTTGAA CCATTTGAAA ATATTGAAGT CTATAACCTA 
ATGTGTGATC TTCTACGCAT TCAACCAGCA CCAAACAATG GAACCCATGG TAGTTTAAAC 
CATCTTCTGA AGGTGCCTTT TTATGAGCCA TCCCATGCAG AGGAGGTGTC AAAGTTTTCT 
GTTTGTGGCT TTGCTAATCC ATTGCCCACA GAGTCTCTTG ACTGTTTCTG CCCTCACCTA 
CAAAATAGTA CTCAGCTGGA ACAAGTGAAT CAGATGCTAA ATCTCACCCA AGAAGAAATA 
ACAGCAACAG TGAAAGTAAA TTTGCCATTT GGGAGGCCTA GGGTACTGCA GAAGAACGTG 
GACCACTGTC TCCTTTACCA CAGGGAATAT GTCAGTGGAT TTGGAAAAGC TATGAGGATG 
CCCATGTGGA GTTCATACAC AGTCCCCCAG TTGGGAGACA CATCGCCTCT GCCTCCCACT 
GTCCCAGACT GTCTGCGGGC TGATGTCAGG GTTCCTCCTT CTGAGAGCCA AAAATGTTCC 
TTCTATTTAG CAGACAAGAA TATCACCCAC GGCTTCCTCT ATCCTCCTGC CAGCAATAGA 
ACATCAGATA GCCAATATGA TGCTTTAATT ACTAGCAATT TGGTACCTAT GTATGAAGAA 
TTCAGAAAAA TGTGGGACTA CTTCCACAGT GTTCTTCTTA TAAAACATGC CACAGAAAGA 
AATGGAGTAA ATGTGGTTAG TGGACCAATA TTTGATTATA ATTATGATGG CCATTTTGAT 
GCTCCAGATG AAATTACCAA ACATTTAGCC AACACTGATG TTCCCATCCC AACACACTAC 
TTTGTGGTGC TGACCAGTTG TAAAAACAAG AGCCACACAC CGGAAAACTG CCCTGGGTGG 
CTGGATGTCC TACCCTTTAT CATCCCTCAC CGACCTACCA ACGTGGAGAG CTGTCCTGAA 
GGTAAACCAG AAGCTCTTTG GGTTGAAGAA AGATTTACAG CTCACATTGC CCGGGTCCGT 
GATGTAGAAC TTCTCACTGG GCTTGACTTC TATCAGGATA AAGTGCAGCC TGTCTCTGAA 
ATTTTGCAAC TAAAGACATA TTTACCAACA TTTGAAACCA CTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENPP3 ( XM_011535897.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011534199.1 Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), transcript variant X3, mRNA.

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CloneID	OHu59605
Clone ID Related Accession (Same CDS sequence)	XM_011535897.1
Accession Version	XM_011535897.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGA CAGCAATGTA TCAAGGTTTA AAAGCCGCTA CCTACTTTTG GCCCGGATCA 
GAAGTGGCTA TAAATGGCTC CTTTCCTTCC ATATACATGC CTTACAACGG AAGTGTCCCA 
TTTGAAGAGA GGATTTCTAC ACTGTTAAAA TGGCTGGACC TGCCCAAAGC TGAAAGACCC 
AGGTTTTATA CCATGTATTT TGAAGAACCT GATTCCTCTG GACATGCAGG TGGACCAGTC 
AGTGCCAGAG TAATTAAAGC CTTACAGGTA GTAGATCATG CTTTTGGGAT GTTGATGGAA 
GGCCTGAAGC AGCGGAATTT GCACAACTGT GTCAATATCA TCCTTCTGGC TGACCATGGA 
ATGGACCAGA CTTATTGTAA CAAGATGGAA TACATGACTG ATTATTTTCC CAGAATAAAC 
TTCTTCTACA TGTACGAAGG GCCTGCCCCC CGCATCCGAG CTCATAATAT ACCTCATGAC 
TTTTTTAGTT TTAATTCTGA GGAAATTGTT AGAAACCTCA GTTGCCGAAA ACCTGATCAG 
CATTTCAAGC CCTATTTGAC TCCTGATTTG CCAAAGCGAC TGCACTATGC CAAGAACGTC 
AGAATCGACA AAGTTCATCT CTTTGTGGAT CAACAGTGGC TGGCTGTTAG GAGTAAATCA 
AATACAAATT GTGGAGGAGG CAACCATGGT TATAACAATG AGTTTAGGAG CATGGAGGCT 
ATCTTTCTGG CACATGGACC CAGTTTTAAA GAGAAGACTG AAGTTGAACC ATTTGAAAAT 
ATTGAAGTCT ATAACCTAAT GTGTGATCTT CTACGCATTC AACCAGCACC AAACAATGGA 
ACCCATGGTA GTTTAAACCA TCTTCTGAAG GTGCCTTTTT ATGAGCCATC CCATGCAGAG 
GAGGTGTCAA AGTTTTCTGT TTGTGGCTTT GCTAATCCAT TGCCCACAGA GTCTCTTGAC 
TGTTTCTGCC CTCACCTACA AAATAGTACT CAGCTGGAAC AAGTGAATCA GATGCTAAAT 
CTCACCCAAG AAGAAATAAC AGCAACAGTG AAAGTAAATT TGCCATTTGG GAGGCCTAGG 
GTACTGCAGA AGAACGTGGA CCACTGTCTC CTTTACCACA GGGAATATGT CAGTGGATTT 
GGAAAAGCTA TGAGGATGCC CATGTGGAGT TCATACACAG TCCCCCAGTT GGGAGACACA 
TCGCCTCTGC CTCCCACTGT CCCAGACTGT CTGCGGGCTG ATGTCAGGGT TCCTCCTTCT 
GAGAGCCAAA AATGTTCCTT CTATTTAGCA GACAAGAATA TCACCCACGG CTTCCTCTAT 
CCTCCTGCCA GCAATAGAAC ATCAGATAGC CAATATGATG CTTTAATTAC TAGCAATTTG 
GTACCTATGT ATGAAGAATT CAGAAAAATG TGGGACTACT TCCACAGTGT TCTTCTTATA 
AAACATGCCA CAGAAAGAAA TGGAGTAAAT GTGGTTAGTG GACCAATATT TGATTATAAT 
TATGATGGCC ATTTTGATGC TCCAGATGAA ATTACCAAAC ATTTAGCCAA CACTGATGTT 
CCCATCCCAA CACACTACTT TGTGGTGCTG ACCAGTTGTA AAAACAAGAG CCACACACCG 
GAAAACTGCC CTGGGTGGCT GGATGTCCTA CCCTTTATCA TCCCTCACCG ACCTACCAAC 
GTGGAGAGCT GTCCTGAAGG TAAACCAGAA GCTCTTTGGG TTGAAGAAAG ATTTACAGCT 
CACATTGCCC GGGTCCGTGA TGTAGAACTT CTCACTGGGC TTGACTTCTA TCAGGATAAA 
GTGCAGCCTG TCTCTGAAAT TTTGCAACTA AAGACATATT TACCAACATT TGAAACCACT 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534199.1
CDS	738..2603
Translation	MWLTAMYQGLKAATYFWPGSEVAINGSFPSIYMPYNGSVPFEERISTLLKWLDLPKAERPRFYTMYFEEPDSSGHAGGPVSARVIKALQVVDHAFGMLMEGLKQRNLHNCVNIILLADHGMDQTYCNKMEYMTDYFPRINFFYMYEGPAPRIRAHNIPHDFFSFNSEEIVRNLSCRKPDQHFKPYLTPDLPKRLHYAKNVRIDKVHLFVDQQWLAVRSKSNTNCGGGNHGYNNEFRSMEAIFLAHGPSFKEKTEVEPFENIEVYNLMCDLLRIQPAPNNGTHGSLNHLLKVPFYEPSHAEEVSKFSVCGFANPLPTESLDCFCPHLQNSTQLEQVNQMLNLTQEEITATVKVNLPFGRPRVLQKNVDHCLLYHREYVSGFGKAMRMPMWSSYTVPQLGDTSPLPPTVPDCLRADVRVPPSESQKCSFYLADKNITHGFLYPPASNRTSDSQYDALITSNLVPMYEEFRKMWDYFHSVLLIKHATERNGVNVVSGPIFDYNYDGHFDAPDEITKHLANTDVPIPTHYFVVLTSCKNKSHTPENCPGWLDVLPFIIPHRPTNVESCPEGKPEALWVEERFTAHIARVRDVELLTGLDFYQDKVQPVSEILQLKTYLPTFETTI

Target ORF information:

RefSeq Version	XM_011535897.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535897.1

ORF Insert Sequence:

ATGTGGCTGA CAGCAATGTA TCAAGGTTTA AAAGCCGCTA CCTACTTTTG GCCCGGATCA 
GAAGTGGCTA TAAATGGCTC CTTTCCTTCC ATATACATGC CTTACAACGG AAGTGTCCCA 
TTTGAAGAGA GGATTTCTAC ACTGTTAAAA TGGCTGGACC TGCCCAAAGC TGAAAGACCC 
AGGTTTTATA CCATGTATTT TGAAGAACCT GATTCCTCTG GACATGCAGG TGGACCAGTC 
AGTGCCAGAG TAATTAAAGC CTTACAGGTA GTAGATCATG CTTTTGGGAT GTTGATGGAA 
GGCCTGAAGC AGCGGAATTT GCACAACTGT GTCAATATCA TCCTTCTGGC TGACCATGGA 
ATGGACCAGA CTTATTGTAA CAAGATGGAA TACATGACTG ATTATTTTCC CAGAATAAAC 
TTCTTCTACA TGTACGAAGG GCCTGCCCCC CGCATCCGAG CTCATAATAT ACCTCATGAC 
TTTTTTAGTT TTAATTCTGA GGAAATTGTT AGAAACCTCA GTTGCCGAAA ACCTGATCAG 
CATTTCAAGC CCTATTTGAC TCCTGATTTG CCAAAGCGAC TGCACTATGC CAAGAACGTC 
AGAATCGACA AAGTTCATCT CTTTGTGGAT CAACAGTGGC TGGCTGTTAG GAGTAAATCA 
AATACAAATT GTGGAGGAGG CAACCATGGT TATAACAATG AGTTTAGGAG CATGGAGGCT 
ATCTTTCTGG CACATGGACC CAGTTTTAAA GAGAAGACTG AAGTTGAACC ATTTGAAAAT 
ATTGAAGTCT ATAACCTAAT GTGTGATCTT CTACGCATTC AACCAGCACC AAACAATGGA 
ACCCATGGTA GTTTAAACCA TCTTCTGAAG GTGCCTTTTT ATGAGCCATC CCATGCAGAG 
GAGGTGTCAA AGTTTTCTGT TTGTGGCTTT GCTAATCCAT TGCCCACAGA GTCTCTTGAC 
TGTTTCTGCC CTCACCTACA AAATAGTACT CAGCTGGAAC AAGTGAATCA GATGCTAAAT 
CTCACCCAAG AAGAAATAAC AGCAACAGTG AAAGTAAATT TGCCATTTGG GAGGCCTAGG 
GTACTGCAGA AGAACGTGGA CCACTGTCTC CTTTACCACA GGGAATATGT CAGTGGATTT 
GGAAAAGCTA TGAGGATGCC CATGTGGAGT TCATACACAG TCCCCCAGTT GGGAGACACA 
TCGCCTCTGC CTCCCACTGT CCCAGACTGT CTGCGGGCTG ATGTCAGGGT TCCTCCTTCT 
GAGAGCCAAA AATGTTCCTT CTATTTAGCA GACAAGAATA TCACCCACGG CTTCCTCTAT 
CCTCCTGCCA GCAATAGAAC ATCAGATAGC CAATATGATG CTTTAATTAC TAGCAATTTG 
GTACCTATGT ATGAAGAATT CAGAAAAATG TGGGACTACT TCCACAGTGT TCTTCTTATA 
AAACATGCCA CAGAAAGAAA TGGAGTAAAT GTGGTTAGTG GACCAATATT TGATTATAAT 
TATGATGGCC ATTTTGATGC TCCAGATGAA ATTACCAAAC ATTTAGCCAA CACTGATGTT 
CCCATCCCAA CACACTACTT TGTGGTGCTG ACCAGTTGTA AAAACAAGAG CCACACACCG 
GAAAACTGCC CTGGGTGGCT GGATGTCCTA CCCTTTATCA TCCCTCACCG ACCTACCAAC 
GTGGAGAGCT GTCCTGAAGG TAAACCAGAA GCTCTTTGGG TTGAAGAAAG ATTTACAGCT 
CACATTGCCC GGGTCCGTGA TGTAGAACTT CTCACTGGGC TTGACTTCTA TCAGGATAAA 
GTGCAGCCTG TCTCTGAAAT TTTGCAACTA AAGACATATT TACCAACATT TGAAACCACT 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENPP3 ( XM_017010932.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_016866421.1 Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), transcript variant X1, mRNA.

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CloneID	OHu96648
Clone ID Related Accession (Same CDS sequence)	XM_017010932.1
Accession Version	XM_017010932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTAT TCCACAGGGC ACAACTTGAG AAATGCTGTT CTCTAGCTCG AATATGGATG 
TGCAATAAAT TTCGTTGTGG AGAGACCAGA TTAGAGGCCA GCCTTTGCTC TTGTTCAGAT 
GACTGTTTGC AGAGGAAAGA TTGCTGTGCT GACTATAAGA GTGTTTGCCA AGGAGAAACC 
TCATGGCTGG AAGAAAACTG TGACACAGCC CAGCAGTCTC AGTGCCCAGA AGGGTTTGAC 
CTGCCACCAG TTATCTTGTT TTCTATGGAT GGATTTAGAG CTGAATATTT ATACACATGG 
GATACTTTAA TGCCAAATAT CAATAAACTG AAAACATGTG GAATTCATTC AAAATACATG 
AGAGCTATGT ATCCTACCAA AACCTTCCCA AATCATTACA CCATTGTCAC GGGCTTGTAT 
CCAGAGTCAC ATGGCATCAT TGACAATAAT ATGTATGATG TAAATCTCAA CAAGAATTTT 
TCACTTTCTT CAAAGGAACA AAATAATCCA GCCTGGTGGC ATGGGCAACC AATGTGGCTG 
ACAGCAATGT ATCAAGGTTT AAAAGCCGCT ACCTACTTTT GGCCCGGATC AGAAGTGGCT 
ATAAATGGCT CCTTTCCTTC CATATACATG CCTTACAACG GAAGTGTCCC ATTTGAAGAG 
AGGATTTCTA CACTGTTAAA ATGGCTGGAC CTGCCCAAAG CTGAAAGACC CAGGTTTTAT 
ACCATGTATT TTGAAGAACC TGATTCCTCT GGACATGCAG GTGGACCAGT CAGTGCCAGA 
GTAATTAAAG CCTTACAGGT AGTAGATCAT GCTTTTGGGA TGTTGATGGA AGGCCTGAAG 
CAGCGGAATT TGCACAACTG TGTCAATATC ATCCTTCTGG CTGACCATGG AATGGACCAG 
ACTTATTGTA ACAAGATGGA ATACATGACT GATTATTTTC CCAGAATAAA CTTCTTCTAC 
ATGTACGAAG GGCCTGCCCC CCGCATCCGA GCTCATAATA TACCTCATGA CTTTTTTAGT 
TTTAATTCTG AGGAAATTGT TAGAAACCTC AGTTGCCGAA AACCTGATCA GCATTTCAAG 
CCCTATTTGA CTCCTGATTT GCCAAAGCGA CTGCACTATG CCAAGAACGT CAGAATCGAC 
AAAGTTCATC TCTTTGTGGA TCAACAGTGG CTGGCTGTTA GGAGTAAATC AAATACAAAT 
TGTGGAGGAG GCAACCATGG TTATAACAAT GAGTTTAGGA GCATGGAGGC TATCTTTCTG 
GCACATGGAC CCAGTTTTAA AGAGAAGACT GAAGTTGAAC CATTTGAAAA TATTGAAGTC 
TATAACCTAA TGTGTGATCT TCTACGCATT CAACCAGCAC CAAACAATGG AACCCATGGT 
AGTTTAAACC ATCTTCTGAA GGTGCCTTTT TATGAGCCAT CCCATGCAGA GGAGGTGTCA 
AAGTTTTCTG TTTGTGGCTT TGCTAATCCA TTGCCCACAG AGTCTCTTGA CTGTTTCTGC 
CCTCACCTAC AAAATAGTAC TCAGCTGGAA CAAGTGAATC AGATGCTAAA TCTCACCCAA 
GAAGAAATAA CAGCAACAGT GAAAGTAAAT TTGCCATTTG GGAGGCCTAG GGTACTGCAG 
AAGAACGTGG ACCACTGTCT CCTTTACCAC AGGGAATATG TCAGTGGATT TGGAAAAGCT 
ATGAGGATGC CCATGTGGAG TTCATACACA GTCCCCCAGT TGGGAGACAC ATCGCCTCTG 
CCTCCCACTG TCCCAGACTG TCTGCGGGCT GATGTCAGGG TTCCTCCTTC TGAGAGCCAA 
AAATGTTCCT TCTATTTAGC AGACAAGAAT ATCACCCACG GCTTCCTCTA TCCTCCTGCC 
AGCAATAGAA CATCAGATAG CCAATATGAT GCTTTAATTA CTAGCAATTT GGTACCTATG 
TATGAAGAAT TCAGAAAAAT GTGGGACTAC TTCCACAGTG TTCTTCTTAT AAAACATGCC 
ACAGAAAGAA ATGGAGTAAA TGTGGTTAGT GGACCAATAT TTGATTATAA TTATGATGGC 
CATTTTGATG CTCCAGATGA AATTACCAAA CATTTAGCCA ACACTGATGT TCCCATCCCA 
ACACACTACT TTGTGGTGCT GACCAGTTGT AAAAACAAGA GCCACACACC GGAAAACTGC 
CCTGGGTGGC TGGATGTCCT ACCCTTTATC ATCCCTCACC GACCTACCAA CGTGGAGAGC 
TGTCCTGAAG GTAAACCAGA AGCTCTTTGG GTTGAAGAAA GATTTACAGC TCACATTGCC 
CGGGTCCGTG ATGTAGAACT TCTCACTGGG CTTGACTTCT ATCAGGATAA AGTGCAGCCT 
GTCTCTGAAA TTTTGCAACT AAAGACATAT TTACCAACAT TTGAAACCAC TATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866421.1
CDS	427..2823
Translation	MPVFHRAQLEKCCSLARIWMCNKFRCGETRLEASLCSCSDDCLQRKDCCADYKSVCQGETSWLEENCDTAQQSQCPEGFDLPPVILFSMDGFRAEYLYTWDTLMPNINKLKTCGIHSKYMRAMYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVNLNKNFSLSSKEQNNPAWWHGQPMWLTAMYQGLKAATYFWPGSEVAINGSFPSIYMPYNGSVPFEERISTLLKWLDLPKAERPRFYTMYFEEPDSSGHAGGPVSARVIKALQVVDHAFGMLMEGLKQRNLHNCVNIILLADHGMDQTYCNKMEYMTDYFPRINFFYMYEGPAPRIRAHNIPHDFFSFNSEEIVRNLSCRKPDQHFKPYLTPDLPKRLHYAKNVRIDKVHLFVDQQWLAVRSKSNTNCGGGNHGYNNEFRSMEAIFLAHGPSFKEKTEVEPFENIEVYNLMCDLLRIQPAPNNGTHGSLNHLLKVPFYEPSHAEEVSKFSVCGFANPLPTESLDCFCPHLQNSTQLEQVNQMLNLTQEEITATVKVNLPFGRPRVLQKNVDHCLLYHREYVSGFGKAMRMPMWSSYTVPQLGDTSPLPPTVPDCLRADVRVPPSESQKCSFYLADKNITHGFLYPPASNRTSDSQYDALITSNLVPMYEEFRKMWDYFHSVLLIKHATERNGVNVVSGPIFDYNYDGHFDAPDEITKHLANTDVPIPTHYFVVLTSCKNKSHTPENCPGWLDVLPFIIPHRPTNVESCPEGKPEALWVEERFTAHIARVRDVELLTGLDFYQDKVQPVSEILQLKTYLPTFETTI

Target ORF information:

RefSeq Version	XM_017010932.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010932.1

ORF Insert Sequence:

ATGCCAGTAT TCCACAGGGC ACAACTTGAG AAATGCTGTT CTCTAGCTCG AATATGGATG 
TGCAATAAAT TTCGTTGTGG AGAGACCAGA TTAGAGGCCA GCCTTTGCTC TTGTTCAGAT 
GACTGTTTGC AGAGGAAAGA TTGCTGTGCT GACTATAAGA GTGTTTGCCA AGGAGAAACC 
TCATGGCTGG AAGAAAACTG TGACACAGCC CAGCAGTCTC AGTGCCCAGA AGGGTTTGAC 
CTGCCACCAG TTATCTTGTT TTCTATGGAT GGATTTAGAG CTGAATATTT ATACACATGG 
GATACTTTAA TGCCAAATAT CAATAAACTG AAAACATGTG GAATTCATTC AAAATACATG 
AGAGCTATGT ATCCTACCAA AACCTTCCCA AATCATTACA CCATTGTCAC GGGCTTGTAT 
CCAGAGTCAC ATGGCATCAT TGACAATAAT ATGTATGATG TAAATCTCAA CAAGAATTTT 
TCACTTTCTT CAAAGGAACA AAATAATCCA GCCTGGTGGC ATGGGCAACC AATGTGGCTG 
ACAGCAATGT ATCAAGGTTT AAAAGCCGCT ACCTACTTTT GGCCCGGATC AGAAGTGGCT 
ATAAATGGCT CCTTTCCTTC CATATACATG CCTTACAACG GAAGTGTCCC ATTTGAAGAG 
AGGATTTCTA CACTGTTAAA ATGGCTGGAC CTGCCCAAAG CTGAAAGACC CAGGTTTTAT 
ACCATGTATT TTGAAGAACC TGATTCCTCT GGACATGCAG GTGGACCAGT CAGTGCCAGA 
GTAATTAAAG CCTTACAGGT AGTAGATCAT GCTTTTGGGA TGTTGATGGA AGGCCTGAAG 
CAGCGGAATT TGCACAACTG TGTCAATATC ATCCTTCTGG CTGACCATGG AATGGACCAG 
ACTTATTGTA ACAAGATGGA ATACATGACT GATTATTTTC CCAGAATAAA CTTCTTCTAC 
ATGTACGAAG GGCCTGCCCC CCGCATCCGA GCTCATAATA TACCTCATGA CTTTTTTAGT 
TTTAATTCTG AGGAAATTGT TAGAAACCTC AGTTGCCGAA AACCTGATCA GCATTTCAAG 
CCCTATTTGA CTCCTGATTT GCCAAAGCGA CTGCACTATG CCAAGAACGT CAGAATCGAC 
AAAGTTCATC TCTTTGTGGA TCAACAGTGG CTGGCTGTTA GGAGTAAATC AAATACAAAT 
TGTGGAGGAG GCAACCATGG TTATAACAAT GAGTTTAGGA GCATGGAGGC TATCTTTCTG 
GCACATGGAC CCAGTTTTAA AGAGAAGACT GAAGTTGAAC CATTTGAAAA TATTGAAGTC 
TATAACCTAA TGTGTGATCT TCTACGCATT CAACCAGCAC CAAACAATGG AACCCATGGT 
AGTTTAAACC ATCTTCTGAA GGTGCCTTTT TATGAGCCAT CCCATGCAGA GGAGGTGTCA 
AAGTTTTCTG TTTGTGGCTT TGCTAATCCA TTGCCCACAG AGTCTCTTGA CTGTTTCTGC 
CCTCACCTAC AAAATAGTAC TCAGCTGGAA CAAGTGAATC AGATGCTAAA TCTCACCCAA 
GAAGAAATAA CAGCAACAGT GAAAGTAAAT TTGCCATTTG GGAGGCCTAG GGTACTGCAG 
AAGAACGTGG ACCACTGTCT CCTTTACCAC AGGGAATATG TCAGTGGATT TGGAAAAGCT 
ATGAGGATGC CCATGTGGAG TTCATACACA GTCCCCCAGT TGGGAGACAC ATCGCCTCTG 
CCTCCCACTG TCCCAGACTG TCTGCGGGCT GATGTCAGGG TTCCTCCTTC TGAGAGCCAA 
AAATGTTCCT TCTATTTAGC AGACAAGAAT ATCACCCACG GCTTCCTCTA TCCTCCTGCC 
AGCAATAGAA CATCAGATAG CCAATATGAT GCTTTAATTA CTAGCAATTT GGTACCTATG 
TATGAAGAAT TCAGAAAAAT GTGGGACTAC TTCCACAGTG TTCTTCTTAT AAAACATGCC 
ACAGAAAGAA ATGGAGTAAA TGTGGTTAGT GGACCAATAT TTGATTATAA TTATGATGGC 
CATTTTGATG CTCCAGATGA AATTACCAAA CATTTAGCCA ACACTGATGT TCCCATCCCA 
ACACACTACT TTGTGGTGCT GACCAGTTGT AAAAACAAGA GCCACACACC GGAAAACTGC 
CCTGGGTGGC TGGATGTCCT ACCCTTTATC ATCCCTCACC GACCTACCAA CGTGGAGAGC 
TGTCCTGAAG GTAAACCAGA AGCTCTTTGG GTTGAAGAAA GATTTACAGC TCACATTGCC 
CGGGTCCGTG ATGTAGAACT TCTCACTGGG CTTGACTTCT ATCAGGATAA AGTGCAGCCT 
GTCTCTGAAA TTTTGCAACT AAAGACATAT TTACCAACAT TTGAAACCAC TATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Blood basophil activation is a reliable biomarker of allergic bronchopulmonary aspergillosis in cystic fibrosis.
The European respiratory journal47(1)177-85(2016 Jan)
Gernez Y,Walters J,Mirkovi? B,Lavelle GM,Colleen DE,Davies ZA,Everson C,Tirouvanziam R,Silver E,Wallenstein S,Chotirmall SH,McElvaney NG,Herzenberg LA,Moss RB

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ENPP3 cDNA ORF clone, Homo sapiens(Human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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