LARS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LARS1
Entrez Gene ID	51520
Full Name	leucyl-tRNA synthetase
Synonyms	HSPC192,ILFS1,LARS1,LEURS,LEUS,LFIS,LRS,PIG44,RNTLS,hr025Cl
General protein information	Preferred Names leucyl-tRNA synthetase Names leucine--tRNA ligase, cytoplasmic cytoplasmic leucyl-tRNA synthetase cytosolic leucyl-tRNA synthetase leucine tRNA ligase 1, cytoplasmic leucine translase proliferation-inducing gene 44
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q32
Summary	This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. A mutation in this gene was found in affected individuals with infantile liver failure syndrome 1. Alternatively spliced transcript variants of this gene have been observed. [provided by RefSeq, Dec 2015].
Disorder MIM:	151350
Disorder Html:	?Infantile liver failure syndrome 1, 615438 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001317965.1	NP_001304894.1	leucine--tRNA ligase, cytoplasmic isoform 4
XM_011537656.3	XP_011535958.1	leucine--tRNA ligase, cytoplasmic isoform X1
XM_011537656.2	XP_011535958.1	leucine--tRNA ligase, cytoplasmic isoform X1
NM_020117.10	NP_064502.9	leucine--tRNA ligase, cytoplasmic isoform 1
NM_001317964.1	NP_001304893.1	leucine--tRNA ligase, cytoplasmic isoform 3
NM_020117.11	NP_064502.9	leucine--tRNA ligase, cytoplasmic isoform 1
NM_020117.9	NP_064502.9	leucine--tRNA ligase, cytoplasmic
NM_016460.3	NP_057544.2	leucine--tRNA ligase, cytoplasmic isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	LARS	NP_064502.9
Drosophila melanogaster (fruit fly)	CG33123	NP_787968.1
Neurospora crassa	NCU09463	XP_963218.1
Oryza sativa (rice)	Os09g0378300	NP_001063048.1
Rattus norvegicus (Norway rat)	Lars	NP_001009637.1
Danio rerio (zebrafish)	larsb	XP_698279.5
Caenorhabditis elegans (roundworm)	lars-1	NP_497837.1
Saccharomyces cerevisiae (baker's yeast)	CDC60	NP_015165.1
Arabidopsis thaliana (thale cress)	AT1G09620	NP_172433.2
Oryza sativa (rice)	Os09g0503400	NP_001063593.2
Xenopus tropicalis (tropical clawed frog)	lars	NP_001015831.1
Canis lupus familiaris (dog)	LARS	XP_535229.3
Mus musculus (house mouse)	Lars	NP_598898.2
Gallus gallus (chicken)	LARS	XP_414663.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008297	XP_317169.2
Kluyveromyces lactis	KLLA0D06105g	XP_453334.1
Magnaporthe oryzae (rice blast fungus)	MGG_02432	XP_003709219.1
Pan troglodytes (chimpanzee)	LARS	XP_001157096.1
Bos taurus (cattle)	LARS	NP_001095962.1
Eremothecium gossypii	AGOS_AAL088W	NP_982454.2
Schizosaccharomyces pombe (fission yeast)	lrs1	NP_594882.1

Gene Ontology
Bibliography

Related articles in PubMed

Leucine-induced localization of Leucyl-tRNA synthetase in lysosome membrane.
Choi H, Son JB, Kang J, Kwon J, Kim JH, Jung M, Kim SK, Kim S, Mun JY
Biochemical and biophysical research communications493(2)1129-1135(2017 11)

Control of leucine-dependent mTORC1 pathway through chemical intervention of leucyl-tRNA synthetase and RagD interaction.
Kim JH, Lee C, Lee M, Wang H, Kim K, Park SJ, Yoon I, Jang J, Zhao H, Kim HK, Kwon NH, Jeong SJ, Yoo HC, Kim JH, Yang JS, Lee MY, Lee CW, Yun J, Oh SJ, Kang JS, Martinis SA, Hwang KY, Guo M, Han G, Han JM, Kim S
Nature communications8(1)732(2017 09)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

The crystal structure of human GlnRS provides basis for the development of neurological disorders.
Ognjenovi? J, Wu J, Matthies D, Baxa U, Subramaniam S, Ling J, Simonovi? M
Nucleic acids research44(7)3420-31(2016 Apr)

Modulation of Aminoacylation and Editing Properties of Leucyl-tRNA Synthetase by a Conserved Structural Module.
Yan W, Ye Q, Tan M, Chen X, Eriani G, Wang ED
The Journal of biological chemistry290(19)12256-67(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results showed a decrease in autophagy on addition of leucine, demonstrating crosstalk between leucine sensing, LRS translocation, RagD interaction, and mTORC1 activation.
Title: Leucine-induced localization of Leucyl-tRNA synthetase in lysosome membrane.

the KMSKS catalytic loop affects the aminoacylation and editing capacities of leucyl-tRNA synthetase
Title: Modulation of Aminoacylation and Editing Properties of Leucyl-tRNA Synthetase by a Conserved Structural Module.

Lack of a CP1 hairpin in LeuRS led to complete loss of aminoacylation, amino acid activation, and tRNA binding; however, the mutants retained post-transfer editing.
Title: A bridge between the aminoacylation and editing domains of leucyl-tRNA synthetase is crucial for its synthetic activity.

the carboxy-terminal domain of human mitochondrial (mt) leucyl-tRNA synthetase can be used to correct mt dysfunctions caused by mt-tRNA mutations.
Title: The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells.

Identification of a mutation in LARS as a novel cause of infantile hepatopathy
Title: Identification of a mutation in LARS as a novel cause of infantile hepatopathy.

The following LARS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LARS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03199	NM_020117.11 Latest version!	Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu67988	NM_001317965.1 Latest version!	Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu67988	XM_011537656.3 Latest version!	Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu101697	NM_016460.3 Latest version!	Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu67987	NM_001317964.1 Latest version!	Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu67988	XM_011537656.2	Homo sapiens leucyl-tRNA synthetase (LARS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu03199	NM_020117.10	Homo sapiens leucyl-tRNA synthetase (LARS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu03199	NM_020117.9	Homo sapiens leucyl-tRNA synthetase (LARS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LARS1 ( NM_020117.10 ) cDNA ORF clone, Homo sapiens(human) -> NP_064502.9 Homo sapiens leucyl-tRNA synthetase (LARS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03199
Clone ID Related Accession (Same CDS sequence)	NM_020117.10 , NM_020117.11 , NM_020117.9
Accession Version	NM_020117.10	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-02
Organism	Homo sapiens(human)
Product	leucine--tRNA ligase, cytoplasmic isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB091795.1, AB037773.1, AC091887.3 and CK902545.1. This sequence is a reference standard in the RefSeqGene project. On Dec 17, 2015 this sequence version replaced NM_020117.9. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.162211.1, SRR1803614.84918.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG 
ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT 
TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA 
GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGGCATGT 
GCTGATAAGT TGAAAAGAGA AATAGAGCTG TATGGTTGCC CCCCTGATTT TCCAGATGAA 
GAAGAGGAAG AGGAAGAAAC CAGTGTTAAA ACAGAAGATA TAATAATTAA GGATAAAGCT 
AAAGGAAAAA AGAGTAAAGC TGCTGCTAAA GCTGGATCTT CTAAATACCA GTGGGGCATT 
ATGAAATCCC TTGGCCTGTC TGATGAAGAG ATAGTAAAAT TTTCTGAAGC AGAACATTGG 
CTTGATTATT TCCCGCCACT GGCTATTCAG GATTTAAAAA GAATGGGTTT GAAGGTAGAC 
TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGATGG 
CAATTTTTAA CATTAAGAGA AAGAAACAAA ATTAAATTTG GGAAGCGGTA TACAATTTAC 
TCTCCGAAAG ATGGACAGCC TTGCATGGAT CATGATAGAC AAACTGGAGA GGGTGTTGGA 
CCTCAGGAAT ATACTTTACT CAAATTGAAG GTGCTTGAGC CATACCCATC TAAATTAAGT 
GGCCTGAAAG GTAAAAATAT TTTCTTGGTG GCTGCTACTC TCAGACCTGA GACCATGTTT 
GGGCAGACAA ATTGTTGGGT TCGTCCTGAT ATGAAGTACA TTGGATTTGA GACGGTGAAT 
GGTGATATAT TCATCTGTAC CCAAAAAGCA GCCAGGAATA TGTCATACCA GGGCTTTACC 
AAAGACAATG GCGTGGTGCC TGTTGTTAAG GAATTAATGG GGGAGGAAAT TCTTGGTGCA 
TCACTTTCTG CACCTTTAAC ATCATACAAG GTGATCTATG TTCTCCCAAT GCTAACTATT 
AAGGAGGATA AAGGCACTGG TGTGGTTACA AGTGTTCCTT CCGACTCCCC TGATGATATT 
GCTGCCCTCA GAGACTTGAA GAAAAAGCAA GCCTTACGAG CAAAATATGG AATTAGAGAT 
GACATGGTCT TGCCATTTGA GCCGGTGCCA GTCATTGAAA TCCCAGGTTT TGGAAATCTT 
TCTGCTGTAA CCATTTGTGA TGAGTTGAAA ATTCAGAGCC AGAATGACCG GGAAAAACTT 
GCAGAAGCAA AGGAGAAGAT ATATCTAAAA GGATTTTATG AGGGTATCAT GTTGGTGGAT 
GGATTTAAAG GACAGAAGGT TCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC 
GCTGGAGATG CACTTATTTA CATGGAACCA GAGAAACAAG TGATGTCCAG GTCGTCAGAT 
GAATGTGTTG TGGCTCTGTG TGACCAGTGG TACTTGGATT ATGGAGAAGA GAATTGGAAG 
AAACAGACAT CTCAGTGCTT GAAGAACCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT 
TTTGAAGCCA CCTTAGGTTG GCTACAAGAA CATGCTTGCT CAAGAACTTA TGGTCTAGGC 
ACTCACCTGC CTTGGGATGA GCAGTGGCTG ATTGAATCAC TTTCTGACTC CACTATTTAC 
ATGGCATTTT ACACAGTTGC ACACCTATTG CAGGGGGGTA ACTTGCATGG ACAGGCAGAG 
TCTCCGCTGG GCATTAGACC GCAACAGATG ACCAAGGAAG TTTGGGATTA TGTTTTCTTC 
AAGGAGGCTC CATTTCCTAA GACTCAGATT GCAAAGGAAA AATTAGATCA GTTAAAGCAG 
GAGTTTGAAT TCTGGTATCC TGTTGATCTT CGCGTCTCTG GCAAGGATCT TGTTCCAAAT 
CATCTTTCAT ATTACCTTTA TAATCATGTG GCTATGTGGC CGGAACAAAG TGACAAATGG 
CCTACAGCTG TGAGAGCAAA TGGACATCTC CTCCTGAACT CTGAGAAGAT GTCAAAATCC 
ACAGGCAACT TCCTCACTTT GACCCAAGCT ATTGACAAAT TTTCAGCAGA TGGAATGCGT 
TTGGCTCTGG CTGATGCTGG TGACACTGTA GAAGATGCCA ACTTTGTGGA AGCCATGGCA 
GATGCAGGTA TTCTCCGTCT GTACACCTGG GTAGAGTGGG TGAAAGAAAT GGTTGCCAAC 
TGGGACAGCC TAAGAAGTGG TCCTGCCAGC ACTTTCAATG ATAGAGTTTT TGCCAGTGAA 
TTGAATGCAG GAATTATAAA AACAGATCAA AACTATGAAA AGATGATGTT TAAAGAAGCT 
TTGAAAACAG GGTTTTTTGA GTTTCAGGCC GCAAAAGATA AGTACCGTGA ATTGGCTGTG 
GAAGGGATGC ACAGAGAACT TGTGTTCCGG TTTATTGAAG TTCAGACACT TCTCCTCGCT 
CCATTCTGTC CACATTTGTG TGAGCACATC TGGACACTCC TGGGAAAGCC TGACTCAATT 
ATGAATGCTT CATGGCCTGT GGCAGGTCCT GTTAATGAAG TTTTAATACA CTCCTCACAG 
TATCTTATGG AAGTAACACA TGACCTTAGA CTACGACTCA AGAACTATAT GATGCCAGCT 
AAAGGGAAGA AGACTGACAA ACAACCCCTG CAGAAGCCCT CACATTGCAC CATCTATGTG 
GCAAAGAACT ATCCACCTTG GCAACATACC ACCCTGTCTG TTCTACGTAA ACACTTTGAG 
GCCAATAACG GAAAACTGCC TGACAACAAA GTCATTGCTA GTGAACTAGG CAGTATGCCA 
GAACTGAAGA AATACATGAA GAAAGTCATG CCATTTGTTG CCATGATTAA GGAAAATCTG 
GAGAAGATGG GGCCTCGTAT TCTGGATTTG CAATTAGAAT TTGATGAAAA GGCTGTGCTT 
ATGGAGAATA TAGTCTATCT GACTAATTCG CTTGAGCTAG AACACATAGA AGTCAAGTTT 
GCCTCCGAAG CAGAAGATAA AATCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT 
TTTAGAATAG AACCTGGTGT GTCCGTTTCT CTGGTGAATC CCCAGCCATC CAATGGCCAC 
TTCTCAACCA AAATTGAAAT CAGGCAAGGA GATAACTGTG ATTCCATAAT CAGGCGTTTA 
ATGAAAATGA ATCGAGGAAT TAAAGACCTT TCCAAAGTGA AACTGATGAG ATTTGATGAT 
CCACTGTTGG GGCCTCGACG AGTTCCTGTC CTGGGAAAGG AGTACACCGA GAAGACCCCC 
ATTTCTGAGC ATGCTGTTTT CAATGTGGAC CTCATGAGCA AGAAAATTCA TCTGACTGAG 
AATGGGATAA GGGTGGATAT TGGCGATACA ATAATCTATC TGGTTCATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064502.9
CDS	295..3825
Translation	MAERKGTAKVDFLKKIEKEIQQKWDTERVFEVNASNLEKQTSKGKYFVTFPYPYMNGRLHLGHTFSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPDEEEEEEETSVKTEDIIIKDKAKGKKSKAAAKAGSSKYQWGIMKSLGLSDEEIVKFSEAEHWLDYFPPLAIQDLKRMGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILGASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIRDDMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDVKKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFCEETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSKSTGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVANWDSLRSGPASTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYRELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVNEVLIHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKLPDNKVIASELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAEDKIREDCCPGKPLNVFRIEPGVSVSLVNPQPSNGHFSTKIEIRQGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDDPLLGPRRVPVLGKEYTEKTPISEHAVFNVDLMSKKIHLTENGIRVDIGDTIIYLVH

Target ORF information:

RefSeq Version	NM_020117.10
Organism	Homo sapiens(human)
Definition	Homo sapiens leucyl-tRNA synthetase (LARS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020117.10

ORF Insert Sequence:

ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG 
ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT 
TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA 
GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGGCATGT 
GCTGATAAGT TGAAAAGAGA AATAGAGCTG TATGGTTGCC CCCCTGATTT TCCAGATGAA 
GAAGAGGAAG AGGAAGAAAC CAGTGTTAAA ACAGAAGATA TAATAATTAA GGATAAAGCT 
AAAGGAAAAA AGAGTAAAGC TGCTGCTAAA GCTGGATCTT CTAAATACCA GTGGGGCATT 
ATGAAATCCC TTGGCCTGTC TGATGAAGAG ATAGTAAAAT TTTCTGAAGC AGAACATTGG 
CTTGATTATT TCCCGCCACT GGCTATTCAG GATTTAAAAA GAATGGGTTT GAAGGTAGAC 
TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGATGG 
CAATTTTTAA CATTAAGAGA AAGAAACAAA ATTAAATTTG GGAAGCGGTA TACAATTTAC 
TCTCCGAAAG ATGGACAGCC TTGCATGGAT CATGATAGAC AAACTGGAGA GGGTGTTGGA 
CCTCAGGAAT ATACTTTACT CAAATTGAAG GTGCTTGAGC CATACCCATC TAAATTAAGT 
GGCCTGAAAG GTAAAAATAT TTTCTTGGTG GCTGCTACTC TCAGACCTGA GACCATGTTT 
GGGCAGACAA ATTGTTGGGT TCGTCCTGAT ATGAAGTACA TTGGATTTGA GACGGTGAAT 
GGTGATATAT TCATCTGTAC CCAAAAAGCA GCCAGGAATA TGTCATACCA GGGCTTTACC 
AAAGACAATG GCGTGGTGCC TGTTGTTAAG GAATTAATGG GGGAGGAAAT TCTTGGTGCA 
TCACTTTCTG CACCTTTAAC ATCATACAAG GTGATCTATG TTCTCCCAAT GCTAACTATT 
AAGGAGGATA AAGGCACTGG TGTGGTTACA AGTGTTCCTT CCGACTCCCC TGATGATATT 
GCTGCCCTCA GAGACTTGAA GAAAAAGCAA GCCTTACGAG CAAAATATGG AATTAGAGAT 
GACATGGTCT TGCCATTTGA GCCGGTGCCA GTCATTGAAA TCCCAGGTTT TGGAAATCTT 
TCTGCTGTAA CCATTTGTGA TGAGTTGAAA ATTCAGAGCC AGAATGACCG GGAAAAACTT 
GCAGAAGCAA AGGAGAAGAT ATATCTAAAA GGATTTTATG AGGGTATCAT GTTGGTGGAT 
GGATTTAAAG GACAGAAGGT TCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC 
GCTGGAGATG CACTTATTTA CATGGAACCA GAGAAACAAG TGATGTCCAG GTCGTCAGAT 
GAATGTGTTG TGGCTCTGTG TGACCAGTGG TACTTGGATT ATGGAGAAGA GAATTGGAAG 
AAACAGACAT CTCAGTGCTT GAAGAACCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT 
TTTGAAGCCA CCTTAGGTTG GCTACAAGAA CATGCTTGCT CAAGAACTTA TGGTCTAGGC 
ACTCACCTGC CTTGGGATGA GCAGTGGCTG ATTGAATCAC TTTCTGACTC CACTATTTAC 
ATGGCATTTT ACACAGTTGC ACACCTATTG CAGGGGGGTA ACTTGCATGG ACAGGCAGAG 
TCTCCGCTGG GCATTAGACC GCAACAGATG ACCAAGGAAG TTTGGGATTA TGTTTTCTTC 
AAGGAGGCTC CATTTCCTAA GACTCAGATT GCAAAGGAAA AATTAGATCA GTTAAAGCAG 
GAGTTTGAAT TCTGGTATCC TGTTGATCTT CGCGTCTCTG GCAAGGATCT TGTTCCAAAT 
CATCTTTCAT ATTACCTTTA TAATCATGTG GCTATGTGGC CGGAACAAAG TGACAAATGG 
CCTACAGCTG TGAGAGCAAA TGGACATCTC CTCCTGAACT CTGAGAAGAT GTCAAAATCC 
ACAGGCAACT TCCTCACTTT GACCCAAGCT ATTGACAAAT TTTCAGCAGA TGGAATGCGT 
TTGGCTCTGG CTGATGCTGG TGACACTGTA GAAGATGCCA ACTTTGTGGA AGCCATGGCA 
GATGCAGGTA TTCTCCGTCT GTACACCTGG GTAGAGTGGG TGAAAGAAAT GGTTGCCAAC 
TGGGACAGCC TAAGAAGTGG TCCTGCCAGC ACTTTCAATG ATAGAGTTTT TGCCAGTGAA 
TTGAATGCAG GAATTATAAA AACAGATCAA AACTATGAAA AGATGATGTT TAAAGAAGCT 
TTGAAAACAG GGTTTTTTGA GTTTCAGGCC GCAAAAGATA AGTACCGTGA ATTGGCTGTG 
GAAGGGATGC ACAGAGAACT TGTGTTCCGG TTTATTGAAG TTCAGACACT TCTCCTCGCT 
CCATTCTGTC CACATTTGTG TGAGCACATC TGGACACTCC TGGGAAAGCC TGACTCAATT 
ATGAATGCTT CATGGCCTGT GGCAGGTCCT GTTAATGAAG TTTTAATACA CTCCTCACAG 
TATCTTATGG AAGTAACACA TGACCTTAGA CTACGACTCA AGAACTATAT GATGCCAGCT 
AAAGGGAAGA AGACTGACAA ACAACCCCTG CAGAAGCCCT CACATTGCAC CATCTATGTG 
GCAAAGAACT ATCCACCTTG GCAACATACC ACCCTGTCTG TTCTACGTAA ACACTTTGAG 
GCCAATAACG GAAAACTGCC TGACAACAAA GTCATTGCTA GTGAACTAGG CAGTATGCCA 
GAACTGAAGA AATACATGAA GAAAGTCATG CCATTTGTTG CCATGATTAA GGAAAATCTG 
GAGAAGATGG GGCCTCGTAT TCTGGATTTG CAATTAGAAT TTGATGAAAA GGCTGTGCTT 
ATGGAGAATA TAGTCTATCT GACTAATTCG CTTGAGCTAG AACACATAGA AGTCAAGTTT 
GCCTCCGAAG CAGAAGATAA AATCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT 
TTTAGAATAG AACCTGGTGT GTCCGTTTCT CTGGTGAATC CCCAGCCATC CAATGGCCAC 
TTCTCAACCA AAATTGAAAT CAGGCAAGGA GATAACTGTG ATTCCATAAT CAGGCGTTTA 
ATGAAAATGA ATCGAGGAAT TAAAGACCTT TCCAAAGTGA AACTGATGAG ATTTGATGAT 
CCACTGTTGG GGCCTCGACG AGTTCCTGTC CTGGGAAAGG AGTACACCGA GAAGACCCCC 
ATTTCTGAGC ATGCTGTTTT CAATGTGGAC CTCATGAGCA AGAAAATTCA TCTGACTGAG 
AATGGGATAA GGGTGGATAT TGGCGATACA ATAATCTATC TGGTTCATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LARS1 ( NM_020117.11 ) cDNA ORF clone, Homo sapiens(human) -> NP_064502.9 Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03199
Clone ID Related Accession (Same CDS sequence)	NM_020117.10 , NM_020117.11 , NM_020117.9
Accession Version	NM_020117.11 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	leucine--tRNA ligase, cytoplasmic isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB037773.1, AC091887.3 and CK902545.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_020117.10. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.12971.1, SRR1803614.84918.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000394434.7/ ENSP00000377954.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG 
ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT 
TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA 
GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGGCATGT 
GCTGATAAGT TGAAAAGAGA AATAGAGCTG TATGGTTGCC CCCCTGATTT TCCAGATGAA 
GAAGAGGAAG AGGAAGAAAC CAGTGTTAAA ACAGAAGATA TAATAATTAA GGATAAAGCT 
AAAGGAAAAA AGAGTAAAGC TGCTGCTAAA GCTGGATCTT CTAAATACCA GTGGGGCATT 
ATGAAATCCC TTGGCCTGTC TGATGAAGAG ATAGTAAAAT TTTCTGAAGC AGAACATTGG 
CTTGATTATT TCCCGCCACT GGCTATTCAG GATTTAAAAA GAATGGGTTT GAAGGTAGAC 
TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGATGG 
CAATTTTTAA CATTAAGAGA AAGAAACAAA ATTAAATTTG GGAAGCGGTA TACAATTTAC 
TCTCCGAAAG ATGGACAGCC TTGCATGGAT CATGATAGAC AAACTGGAGA GGGTGTTGGA 
CCTCAGGAAT ATACTTTACT CAAATTGAAG GTGCTTGAGC CATACCCATC TAAATTAAGT 
GGCCTGAAAG GTAAAAATAT TTTCTTGGTG GCTGCTACTC TCAGACCTGA GACCATGTTT 
GGGCAGACAA ATTGTTGGGT TCGTCCTGAT ATGAAGTACA TTGGATTTGA GACGGTGAAT 
GGTGATATAT TCATCTGTAC CCAAAAAGCA GCCAGGAATA TGTCATACCA GGGCTTTACC 
AAAGACAATG GCGTGGTGCC TGTTGTTAAG GAATTAATGG GGGAGGAAAT TCTTGGTGCA 
TCACTTTCTG CACCTTTAAC ATCATACAAG GTGATCTATG TTCTCCCAAT GCTAACTATT 
AAGGAGGATA AAGGCACTGG TGTGGTTACA AGTGTTCCTT CCGACTCCCC TGATGATATT 
GCTGCCCTCA GAGACTTGAA GAAAAAGCAA GCCTTACGAG CAAAATATGG AATTAGAGAT 
GACATGGTCT TGCCATTTGA GCCGGTGCCA GTCATTGAAA TCCCAGGTTT TGGAAATCTT 
TCTGCTGTAA CCATTTGTGA TGAGTTGAAA ATTCAGAGCC AGAATGACCG GGAAAAACTT 
GCAGAAGCAA AGGAGAAGAT ATATCTAAAA GGATTTTATG AGGGTATCAT GTTGGTGGAT 
GGATTTAAAG GACAGAAGGT TCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC 
GCTGGAGATG CACTTATTTA CATGGAACCA GAGAAACAAG TGATGTCCAG GTCGTCAGAT 
GAATGTGTTG TGGCTCTGTG TGACCAGTGG TACTTGGATT ATGGAGAAGA GAATTGGAAG 
AAACAGACAT CTCAGTGCTT GAAGAACCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT 
TTTGAAGCCA CCTTAGGTTG GCTACAAGAA CATGCTTGCT CAAGAACTTA TGGTCTAGGC 
ACTCACCTGC CTTGGGATGA GCAGTGGCTG ATTGAATCAC TTTCTGACTC CACTATTTAC 
ATGGCATTTT ACACAGTTGC ACACCTATTG CAGGGGGGTA ACTTGCATGG ACAGGCAGAG 
TCTCCGCTGG GCATTAGACC GCAACAGATG ACCAAGGAAG TTTGGGATTA TGTTTTCTTC 
AAGGAGGCTC CATTTCCTAA GACTCAGATT GCAAAGGAAA AATTAGATCA GTTAAAGCAG 
GAGTTTGAAT TCTGGTATCC TGTTGATCTT CGCGTCTCTG GCAAGGATCT TGTTCCAAAT 
CATCTTTCAT ATTACCTTTA TAATCATGTG GCTATGTGGC CGGAACAAAG TGACAAATGG 
CCTACAGCTG TGAGAGCAAA TGGACATCTC CTCCTGAACT CTGAGAAGAT GTCAAAATCC 
ACAGGCAACT TCCTCACTTT GACCCAAGCT ATTGACAAAT TTTCAGCAGA TGGAATGCGT 
TTGGCTCTGG CTGATGCTGG TGACACTGTA GAAGATGCCA ACTTTGTGGA AGCCATGGCA 
GATGCAGGTA TTCTCCGTCT GTACACCTGG GTAGAGTGGG TGAAAGAAAT GGTTGCCAAC 
TGGGACAGCC TAAGAAGTGG TCCTGCCAGC ACTTTCAATG ATAGAGTTTT TGCCAGTGAA 
TTGAATGCAG GAATTATAAA AACAGATCAA AACTATGAAA AGATGATGTT TAAAGAAGCT 
TTGAAAACAG GGTTTTTTGA GTTTCAGGCC GCAAAAGATA AGTACCGTGA ATTGGCTGTG 
GAAGGGATGC ACAGAGAACT TGTGTTCCGG TTTATTGAAG TTCAGACACT TCTCCTCGCT 
CCATTCTGTC CACATTTGTG TGAGCACATC TGGACACTCC TGGGAAAGCC TGACTCAATT 
ATGAATGCTT CATGGCCTGT GGCAGGTCCT GTTAATGAAG TTTTAATACA CTCCTCACAG 
TATCTTATGG AAGTAACACA TGACCTTAGA CTACGACTCA AGAACTATAT GATGCCAGCT 
AAAGGGAAGA AGACTGACAA ACAACCCCTG CAGAAGCCCT CACATTGCAC CATCTATGTG 
GCAAAGAACT ATCCACCTTG GCAACATACC ACCCTGTCTG TTCTACGTAA ACACTTTGAG 
GCCAATAACG GAAAACTGCC TGACAACAAA GTCATTGCTA GTGAACTAGG CAGTATGCCA 
GAACTGAAGA AATACATGAA GAAAGTCATG CCATTTGTTG CCATGATTAA GGAAAATCTG 
GAGAAGATGG GGCCTCGTAT TCTGGATTTG CAATTAGAAT TTGATGAAAA GGCTGTGCTT 
ATGGAGAATA TAGTCTATCT GACTAATTCG CTTGAGCTAG AACACATAGA AGTCAAGTTT 
GCCTCCGAAG CAGAAGATAA AATCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT 
TTTAGAATAG AACCTGGTGT GTCCGTTTCT CTGGTGAATC CCCAGCCATC CAATGGCCAC 
TTCTCAACCA AAATTGAAAT CAGGCAAGGA GATAACTGTG ATTCCATAAT CAGGCGTTTA 
ATGAAAATGA ATCGAGGAAT TAAAGACCTT TCCAAAGTGA AACTGATGAG ATTTGATGAT 
CCACTGTTGG GGCCTCGACG AGTTCCTGTC CTGGGAAAGG AGTACACCGA GAAGACCCCC 
ATTTCTGAGC ATGCTGTTTT CAATGTGGAC CTCATGAGCA AGAAAATTCA TCTGACTGAG 
AATGGGATAA GGGTGGATAT TGGCGATACA ATAATCTATC TGGTTCATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064502.9
CDS	158..3688
Translation	MAERKGTAKVDFLKKIEKEIQQKWDTERVFEVNASNLEKQTSKGKYFVTFPYPYMNGRLHLGHTFSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPDEEEEEEETSVKTEDIIIKDKAKGKKSKAAAKAGSSKYQWGIMKSLGLSDEEIVKFSEAEHWLDYFPPLAIQDLKRMGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILGASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIRDDMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDVKKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFCEETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSKSTGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVANWDSLRSGPASTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYRELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVNEVLIHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKLPDNKVIASELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAEDKIREDCCPGKPLNVFRIEPGVSVSLVNPQPSNGHFSTKIEIRQGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDDPLLGPRRVPVLGKEYTEKTPISEHAVFNVDLMSKKIHLTENGIRVDIGDTIIYLVH

Target ORF information:

RefSeq Version	NM_020117.11
Organism	Homo sapiens(human)
Definition	Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020117.11

ORF Insert Sequence:

ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG 
ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT 
TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA 
GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGGCATGT 
GCTGATAAGT TGAAAAGAGA AATAGAGCTG TATGGTTGCC CCCCTGATTT TCCAGATGAA 
GAAGAGGAAG AGGAAGAAAC CAGTGTTAAA ACAGAAGATA TAATAATTAA GGATAAAGCT 
AAAGGAAAAA AGAGTAAAGC TGCTGCTAAA GCTGGATCTT CTAAATACCA GTGGGGCATT 
ATGAAATCCC TTGGCCTGTC TGATGAAGAG ATAGTAAAAT TTTCTGAAGC AGAACATTGG 
CTTGATTATT TCCCGCCACT GGCTATTCAG GATTTAAAAA GAATGGGTTT GAAGGTAGAC 
TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGATGG 
CAATTTTTAA CATTAAGAGA AAGAAACAAA ATTAAATTTG GGAAGCGGTA TACAATTTAC 
TCTCCGAAAG ATGGACAGCC TTGCATGGAT CATGATAGAC AAACTGGAGA GGGTGTTGGA 
CCTCAGGAAT ATACTTTACT CAAATTGAAG GTGCTTGAGC CATACCCATC TAAATTAAGT 
GGCCTGAAAG GTAAAAATAT TTTCTTGGTG GCTGCTACTC TCAGACCTGA GACCATGTTT 
GGGCAGACAA ATTGTTGGGT TCGTCCTGAT ATGAAGTACA TTGGATTTGA GACGGTGAAT 
GGTGATATAT TCATCTGTAC CCAAAAAGCA GCCAGGAATA TGTCATACCA GGGCTTTACC 
AAAGACAATG GCGTGGTGCC TGTTGTTAAG GAATTAATGG GGGAGGAAAT TCTTGGTGCA 
TCACTTTCTG CACCTTTAAC ATCATACAAG GTGATCTATG TTCTCCCAAT GCTAACTATT 
AAGGAGGATA AAGGCACTGG TGTGGTTACA AGTGTTCCTT CCGACTCCCC TGATGATATT 
GCTGCCCTCA GAGACTTGAA GAAAAAGCAA GCCTTACGAG CAAAATATGG AATTAGAGAT 
GACATGGTCT TGCCATTTGA GCCGGTGCCA GTCATTGAAA TCCCAGGTTT TGGAAATCTT 
TCTGCTGTAA CCATTTGTGA TGAGTTGAAA ATTCAGAGCC AGAATGACCG GGAAAAACTT 
GCAGAAGCAA AGGAGAAGAT ATATCTAAAA GGATTTTATG AGGGTATCAT GTTGGTGGAT 
GGATTTAAAG GACAGAAGGT TCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC 
GCTGGAGATG CACTTATTTA CATGGAACCA GAGAAACAAG TGATGTCCAG GTCGTCAGAT 
GAATGTGTTG TGGCTCTGTG TGACCAGTGG TACTTGGATT ATGGAGAAGA GAATTGGAAG 
AAACAGACAT CTCAGTGCTT GAAGAACCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT 
TTTGAAGCCA CCTTAGGTTG GCTACAAGAA CATGCTTGCT CAAGAACTTA TGGTCTAGGC 
ACTCACCTGC CTTGGGATGA GCAGTGGCTG ATTGAATCAC TTTCTGACTC CACTATTTAC 
ATGGCATTTT ACACAGTTGC ACACCTATTG CAGGGGGGTA ACTTGCATGG ACAGGCAGAG 
TCTCCGCTGG GCATTAGACC GCAACAGATG ACCAAGGAAG TTTGGGATTA TGTTTTCTTC 
AAGGAGGCTC CATTTCCTAA GACTCAGATT GCAAAGGAAA AATTAGATCA GTTAAAGCAG 
GAGTTTGAAT TCTGGTATCC TGTTGATCTT CGCGTCTCTG GCAAGGATCT TGTTCCAAAT 
CATCTTTCAT ATTACCTTTA TAATCATGTG GCTATGTGGC CGGAACAAAG TGACAAATGG 
CCTACAGCTG TGAGAGCAAA TGGACATCTC CTCCTGAACT CTGAGAAGAT GTCAAAATCC 
ACAGGCAACT TCCTCACTTT GACCCAAGCT ATTGACAAAT TTTCAGCAGA TGGAATGCGT 
TTGGCTCTGG CTGATGCTGG TGACACTGTA GAAGATGCCA ACTTTGTGGA AGCCATGGCA 
GATGCAGGTA TTCTCCGTCT GTACACCTGG GTAGAGTGGG TGAAAGAAAT GGTTGCCAAC 
TGGGACAGCC TAAGAAGTGG TCCTGCCAGC ACTTTCAATG ATAGAGTTTT TGCCAGTGAA 
TTGAATGCAG GAATTATAAA AACAGATCAA AACTATGAAA AGATGATGTT TAAAGAAGCT 
TTGAAAACAG GGTTTTTTGA GTTTCAGGCC GCAAAAGATA AGTACCGTGA ATTGGCTGTG 
GAAGGGATGC ACAGAGAACT TGTGTTCCGG TTTATTGAAG TTCAGACACT TCTCCTCGCT 
CCATTCTGTC CACATTTGTG TGAGCACATC TGGACACTCC TGGGAAAGCC TGACTCAATT 
ATGAATGCTT CATGGCCTGT GGCAGGTCCT GTTAATGAAG TTTTAATACA CTCCTCACAG 
TATCTTATGG AAGTAACACA TGACCTTAGA CTACGACTCA AGAACTATAT GATGCCAGCT 
AAAGGGAAGA AGACTGACAA ACAACCCCTG CAGAAGCCCT CACATTGCAC CATCTATGTG 
GCAAAGAACT ATCCACCTTG GCAACATACC ACCCTGTCTG TTCTACGTAA ACACTTTGAG 
GCCAATAACG GAAAACTGCC TGACAACAAA GTCATTGCTA GTGAACTAGG CAGTATGCCA 
GAACTGAAGA AATACATGAA GAAAGTCATG CCATTTGTTG CCATGATTAA GGAAAATCTG 
GAGAAGATGG GGCCTCGTAT TCTGGATTTG CAATTAGAAT TTGATGAAAA GGCTGTGCTT 
ATGGAGAATA TAGTCTATCT GACTAATTCG CTTGAGCTAG AACACATAGA AGTCAAGTTT 
GCCTCCGAAG CAGAAGATAA AATCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT 
TTTAGAATAG AACCTGGTGT GTCCGTTTCT CTGGTGAATC CCCAGCCATC CAATGGCCAC 
TTCTCAACCA AAATTGAAAT CAGGCAAGGA GATAACTGTG ATTCCATAAT CAGGCGTTTA 
ATGAAAATGA ATCGAGGAAT TAAAGACCTT TCCAAAGTGA AACTGATGAG ATTTGATGAT 
CCACTGTTGG GGCCTCGACG AGTTCCTGTC CTGGGAAAGG AGTACACCGA GAAGACCCCC 
ATTTCTGAGC ATGCTGTTTT CAATGTGGAC CTCATGAGCA AGAAAATTCA TCTGACTGAG 
AATGGGATAA GGGTGGATAT TGGCGATACA ATAATCTATC TGGTTCATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LARS1 ( NM_020117.9 ) cDNA ORF clone, Homo sapiens(human) -> NP_064502.9 Homo sapiens leucyl-tRNA synthetase (LARS), mRNA.

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CloneID	OHu03199
Clone ID Related Accession (Same CDS sequence)	NM_020117.10 , NM_020117.11 , NM_020117.9
Accession Version	NM_020117.9	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-29
Organism	Homo sapiens(Human)
Product	leucine--tRNA ligase, cytoplasmic
Comment

ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG 
ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT 
TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA 
GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGGCATGT 
GCTGATAAGT TGAAAAGAGA AATAGAGCTG TATGGTTGCC CCCCTGATTT TCCAGATGAA 
GAAGAGGAAG AGGAAGAAAC CAGTGTTAAA ACAGAAGATA TAATAATTAA GGATAAAGCT 
AAAGGAAAAA AGAGTAAAGC TGCTGCTAAA GCTGGATCTT CTAAATACCA GTGGGGCATT 
ATGAAATCCC TTGGCCTGTC TGATGAAGAG ATAGTAAAAT TTTCTGAAGC AGAACATTGG 
CTTGATTATT TCCCGCCACT GGCTATTCAG GATTTAAAAA GAATGGGTTT GAAGGTAGAC 
TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGATGG 
CAATTTTTAA CATTAAGAGA AAGAAACAAA ATTAAATTTG GGAAGCGGTA TACAATTTAC 
TCTCCGAAAG ATGGACAGCC TTGCATGGAT CATGATAGAC AAACTGGAGA GGGTGTTGGA 
CCTCAGGAAT ATACTTTACT CAAATTGAAG GTGCTTGAGC CATACCCATC TAAATTAAGT 
GGCCTGAAAG GTAAAAATAT TTTCTTGGTG GCTGCTACTC TCAGACCTGA GACCATGTTT 
GGGCAGACAA ATTGTTGGGT TCGTCCTGAT ATGAAGTACA TTGGATTTGA GACGGTGAAT 
GGTGATATAT TCATCTGTAC CCAAAAAGCA GCCAGGAATA TGTCATACCA GGGCTTTACC 
AAAGACAATG GCGTGGTGCC TGTTGTTAAG GAATTAATGG GGGAGGAAAT TCTTGGTGCA 
TCACTTTCTG CACCTTTAAC ATCATACAAG GTGATCTATG TTCTCCCAAT GCTAACTATT 
AAGGAGGATA AAGGCACTGG TGTGGTTACA AGTGTTCCTT CCGACTCCCC TGATGATATT 
GCTGCCCTCA GAGACTTGAA GAAAAAGCAA GCCTTACGAG CAAAATATGG AATTAGAGAT 
GACATGGTCT TGCCATTTGA GCCGGTGCCA GTCATTGAAA TCCCAGGTTT TGGAAATCTT 
TCTGCTGTAA CCATTTGTGA TGAGTTGAAA ATTCAGAGCC AGAATGACCG GGAAAAACTT 
GCAGAAGCAA AGGAGAAGAT ATATCTAAAA GGATTTTATG AGGGTATCAT GTTGGTGGAT 
GGATTTAAAG GACAGAAGGT TCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC 
GCTGGAGATG CACTTATTTA CATGGAACCA GAGAAACAAG TGATGTCCAG GTCGTCAGAT 
GAATGTGTTG TGGCTCTGTG TGACCAGTGG TACTTGGATT ATGGAGAAGA GAATTGGAAG 
AAACAGACAT CTCAGTGCTT GAAGAACCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT 
TTTGAAGCCA CCTTAGGTTG GCTACAAGAA CATGCTTGCT CAAGAACTTA TGGTCTAGGC 
ACTCACCTGC CTTGGGATGA GCAGTGGCTG ATTGAATCAC TTTCTGACTC CACTATTTAC 
ATGGCATTTT ACACAGTTGC ACACCTATTG CAGGGGGGTA ACTTGCATGG ACAGGCAGAG 
TCTCCGCTGG GCATTAGACC GCAACAGATG ACCAAGGAAG TTTGGGATTA TGTTTTCTTC 
AAGGAGGCTC CATTTCCTAA GACTCAGATT GCAAAGGAAA AATTAGATCA GTTAAAGCAG 
GAGTTTGAAT TCTGGTATCC TGTTGATCTT CGCGTCTCTG GCAAGGATCT TGTTCCAAAT 
CATCTTTCAT ATTACCTTTA TAATCATGTG GCTATGTGGC CGGAACAAAG TGACAAATGG 
CCTACAGCTG TGAGAGCAAA TGGACATCTC CTCCTGAACT CTGAGAAGAT GTCAAAATCC 
ACAGGCAACT TCCTCACTTT GACCCAAGCT ATTGACAAAT TTTCAGCAGA TGGAATGCGT 
TTGGCTCTGG CTGATGCTGG TGACACTGTA GAAGATGCCA ACTTTGTGGA AGCCATGGCA 
GATGCAGGTA TTCTCCGTCT GTACACCTGG GTAGAGTGGG TGAAAGAAAT GGTTGCCAAC 
TGGGACAGCC TAAGAAGTGG TCCTGCCAGC ACTTTCAATG ATAGAGTTTT TGCCAGTGAA 
TTGAATGCAG GAATTATAAA AACAGATCAA AACTATGAAA AGATGATGTT TAAAGAAGCT 
TTGAAAACAG GGTTTTTTGA GTTTCAGGCC GCAAAAGATA AGTACCGTGA ATTGGCTGTG 
GAAGGGATGC ACAGAGAACT TGTGTTCCGG TTTATTGAAG TTCAGACACT TCTCCTCGCT 
CCATTCTGTC CACATTTGTG TGAGCACATC TGGACACTCC TGGGAAAGCC TGACTCAATT 
ATGAATGCTT CATGGCCTGT GGCAGGTCCT GTTAATGAAG TTTTAATACA CTCCTCACAG 
TATCTTATGG AAGTAACACA TGACCTTAGA CTACGACTCA AGAACTATAT GATGCCAGCT 
AAAGGGAAGA AGACTGACAA ACAACCCCTG CAGAAGCCCT CACATTGCAC CATCTATGTG 
GCAAAGAACT ATCCACCTTG GCAACATACC ACCCTGTCTG TTCTACGTAA ACACTTTGAG 
GCCAATAACG GAAAACTGCC TGACAACAAA GTCATTGCTA GTGAACTAGG CAGTATGCCA 
GAACTGAAGA AATACATGAA GAAAGTCATG CCATTTGTTG CCATGATTAA GGAAAATCTG 
GAGAAGATGG GGCCTCGTAT TCTGGATTTG CAATTAGAAT TTGATGAAAA GGCTGTGCTT 
ATGGAGAATA TAGTCTATCT GACTAATTCG CTTGAGCTAG AACACATAGA AGTCAAGTTT 
GCCTCCGAAG CAGAAGATAA AATCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT 
TTTAGAATAG AACCTGGTGT GTCCGTTTCT CTGGTGAATC CCCAGCCATC CAATGGCCAC 
TTCTCAACCA AAATTGAAAT CAGGCAAGGA GATAACTGTG ATTCCATAAT CAGGCGTTTA 
ATGAAAATGA ATCGAGGAAT TAAAGACCTT TCCAAAGTGA AACTGATGAG ATTTGATGAT 
CCACTGTTGG GGCCTCGACG AGTTCCTGTC CTGGGAAAGG AGTACACCGA GAAGACCCCC 
ATTTCTGAGC ATGCTGTTTT CAATGTGGAC CTCATGAGCA AGAAAATTCA TCTGACTGAG 
AATGGGATAA GGGTGGATAT TGGCGATACA ATAATCTATC TGGTTCATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_064502.9

Translation

MAERKGTAKVDFLKKIEKEIQQKWDTERVFEVNASNLEKQTSKGKYFVTFPYPYMNGRLHLGHTFSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPDEEEEEEETSVKTEDIIIKDKAKGKKSKAAAKAGSSKYQWGIMKSLGLSDEEIVKFSEAEHWLDYFPPLAIQDLKRMGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILGASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIRDDMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDVKKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFCEETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSKSTGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVANWDSLRSGPASTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYRELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVNEVLIHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKLPDNKVIASELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAEDKIREDCCPGKPLNVFRIEPGVSVSLVNPQPSNGHFSTKIEIRQGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDDPLLGPRRVPVLGKEYTEKTPISEHAVFNVDLMSKKIHLTENGIRVDIGDTIIYLVH

Target ORF information:

RefSeq Version	NM_020117.9
Organism	Homo sapiens(Human)
Definition	Homo sapiens leucyl-tRNA synthetase (LARS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020117.9

ORF Insert Sequence:

ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG 
ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT 
TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA 
GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGGCATGT 
GCTGATAAGT TGAAAAGAGA AATAGAGCTG TATGGTTGCC CCCCTGATTT TCCAGATGAA 
GAAGAGGAAG AGGAAGAAAC CAGTGTTAAA ACAGAAGATA TAATAATTAA GGATAAAGCT 
AAAGGAAAAA AGAGTAAAGC TGCTGCTAAA GCTGGATCTT CTAAATACCA GTGGGGCATT 
ATGAAATCCC TTGGCCTGTC TGATGAAGAG ATAGTAAAAT TTTCTGAAGC AGAACATTGG 
CTTGATTATT TCCCGCCACT GGCTATTCAG GATTTAAAAA GAATGGGTTT GAAGGTAGAC 
TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGATGG 
CAATTTTTAA CATTAAGAGA AAGAAACAAA ATTAAATTTG GGAAGCGGTA TACAATTTAC 
TCTCCGAAAG ATGGACAGCC TTGCATGGAT CATGATAGAC AAACTGGAGA GGGTGTTGGA 
CCTCAGGAAT ATACTTTACT CAAATTGAAG GTGCTTGAGC CATACCCATC TAAATTAAGT 
GGCCTGAAAG GTAAAAATAT TTTCTTGGTG GCTGCTACTC TCAGACCTGA GACCATGTTT 
GGGCAGACAA ATTGTTGGGT TCGTCCTGAT ATGAAGTACA TTGGATTTGA GACGGTGAAT 
GGTGATATAT TCATCTGTAC CCAAAAAGCA GCCAGGAATA TGTCATACCA GGGCTTTACC 
AAAGACAATG GCGTGGTGCC TGTTGTTAAG GAATTAATGG GGGAGGAAAT TCTTGGTGCA 
TCACTTTCTG CACCTTTAAC ATCATACAAG GTGATCTATG TTCTCCCAAT GCTAACTATT 
AAGGAGGATA AAGGCACTGG TGTGGTTACA AGTGTTCCTT CCGACTCCCC TGATGATATT 
GCTGCCCTCA GAGACTTGAA GAAAAAGCAA GCCTTACGAG CAAAATATGG AATTAGAGAT 
GACATGGTCT TGCCATTTGA GCCGGTGCCA GTCATTGAAA TCCCAGGTTT TGGAAATCTT 
TCTGCTGTAA CCATTTGTGA TGAGTTGAAA ATTCAGAGCC AGAATGACCG GGAAAAACTT 
GCAGAAGCAA AGGAGAAGAT ATATCTAAAA GGATTTTATG AGGGTATCAT GTTGGTGGAT 
GGATTTAAAG GACAGAAGGT TCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC 
GCTGGAGATG CACTTATTTA CATGGAACCA GAGAAACAAG TGATGTCCAG GTCGTCAGAT 
GAATGTGTTG TGGCTCTGTG TGACCAGTGG TACTTGGATT ATGGAGAAGA GAATTGGAAG 
AAACAGACAT CTCAGTGCTT GAAGAACCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT 
TTTGAAGCCA CCTTAGGTTG GCTACAAGAA CATGCTTGCT CAAGAACTTA TGGTCTAGGC 
ACTCACCTGC CTTGGGATGA GCAGTGGCTG ATTGAATCAC TTTCTGACTC CACTATTTAC 
ATGGCATTTT ACACAGTTGC ACACCTATTG CAGGGGGGTA ACTTGCATGG ACAGGCAGAG 
TCTCCGCTGG GCATTAGACC GCAACAGATG ACCAAGGAAG TTTGGGATTA TGTTTTCTTC 
AAGGAGGCTC CATTTCCTAA GACTCAGATT GCAAAGGAAA AATTAGATCA GTTAAAGCAG 
GAGTTTGAAT TCTGGTATCC TGTTGATCTT CGCGTCTCTG GCAAGGATCT TGTTCCAAAT 
CATCTTTCAT ATTACCTTTA TAATCATGTG GCTATGTGGC CGGAACAAAG TGACAAATGG 
CCTACAGCTG TGAGAGCAAA TGGACATCTC CTCCTGAACT CTGAGAAGAT GTCAAAATCC 
ACAGGCAACT TCCTCACTTT GACCCAAGCT ATTGACAAAT TTTCAGCAGA TGGAATGCGT 
TTGGCTCTGG CTGATGCTGG TGACACTGTA GAAGATGCCA ACTTTGTGGA AGCCATGGCA 
GATGCAGGTA TTCTCCGTCT GTACACCTGG GTAGAGTGGG TGAAAGAAAT GGTTGCCAAC 
TGGGACAGCC TAAGAAGTGG TCCTGCCAGC ACTTTCAATG ATAGAGTTTT TGCCAGTGAA 
TTGAATGCAG GAATTATAAA AACAGATCAA AACTATGAAA AGATGATGTT TAAAGAAGCT 
TTGAAAACAG GGTTTTTTGA GTTTCAGGCC GCAAAAGATA AGTACCGTGA ATTGGCTGTG 
GAAGGGATGC ACAGAGAACT TGTGTTCCGG TTTATTGAAG TTCAGACACT TCTCCTCGCT 
CCATTCTGTC CACATTTGTG TGAGCACATC TGGACACTCC TGGGAAAGCC TGACTCAATT 
ATGAATGCTT CATGGCCTGT GGCAGGTCCT GTTAATGAAG TTTTAATACA CTCCTCACAG 
TATCTTATGG AAGTAACACA TGACCTTAGA CTACGACTCA AGAACTATAT GATGCCAGCT 
AAAGGGAAGA AGACTGACAA ACAACCCCTG CAGAAGCCCT CACATTGCAC CATCTATGTG 
GCAAAGAACT ATCCACCTTG GCAACATACC ACCCTGTCTG TTCTACGTAA ACACTTTGAG 
GCCAATAACG GAAAACTGCC TGACAACAAA GTCATTGCTA GTGAACTAGG CAGTATGCCA 
GAACTGAAGA AATACATGAA GAAAGTCATG CCATTTGTTG CCATGATTAA GGAAAATCTG 
GAGAAGATGG GGCCTCGTAT TCTGGATTTG CAATTAGAAT TTGATGAAAA GGCTGTGCTT 
ATGGAGAATA TAGTCTATCT GACTAATTCG CTTGAGCTAG AACACATAGA AGTCAAGTTT 
GCCTCCGAAG CAGAAGATAA AATCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT 
TTTAGAATAG AACCTGGTGT GTCCGTTTCT CTGGTGAATC CCCAGCCATC CAATGGCCAC 
TTCTCAACCA AAATTGAAAT CAGGCAAGGA GATAACTGTG ATTCCATAAT CAGGCGTTTA 
ATGAAAATGA ATCGAGGAAT TAAAGACCTT TCCAAAGTGA AACTGATGAG ATTTGATGAT 
CCACTGTTGG GGCCTCGACG AGTTCCTGTC CTGGGAAAGG AGTACACCGA GAAGACCCCC 
ATTTCTGAGC ATGCTGTTTT CAATGTGGAC CTCATGAGCA AGAAAATTCA TCTGACTGAG 
AATGGGATAA GGGTGGATAT TGGCGATACA ATAATCTATC TGGTTCATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LARS1 ( NM_001317965.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304894.1 Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 4, mRNA.

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CloneID	OHu67988
Clone ID Related Accession (Same CDS sequence)	NM_001317965.1 , XM_011537656.3 , XM_011537656.2
Accession Version	NM_001317965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	leucine--tRNA ligase, cytoplasmic isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB091795.1, AK295874.1, AB037773.1, AC091887.3 and CK902545.1. Transcript Variant: This variant (4) lacks an alternate exon in the 5' region and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (4) has a shorter N-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295874.1, SRR1803615.42742.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATGGAC GCCTTCATTT GGGACACACG TTTTCTTTAT CCAAATGTGA GTTTGCTGTA 
GGGTACCAGC GATTGAAAGG AAAATGTTGT CTGTTTCCCT TTGGCCTGCA CTGTACTGGA 
ATGCCTATTA AGGCATGTGC TGATAAGTTG AAAAGAGAAA TAGAGCTGTA TGGTTGCCCC 
CCTGATTTTC CAGATGAAGA AGAGGAAGAG GAAGAAACCA GTGTTAAAAC AGAAGATATA 
ATAATTAAGG ATAAAGCTAA AGGAAAAAAG AGTAAAGCTG CTGCTAAAGC TGGATCTTCT 
AAATACCAGT GGGGCATTAT GAAATCCCTT GGCCTGTCTG ATGAAGAGAT AGTAAAATTT 
TCTGAAGCAG AACATTGGCT TGATTATTTC CCGCCACTGG CTATTCAGGA TTTAAAAAGA 
ATGGGTTTGA AGGTAGACTG GCGTCGTTCC TTCATCACCA CTGATGTTAA TCCTTACTAT 
GATTCATTTG TCAGATGGCA ATTTTTAACA TTAAGAGAAA GAAACAAAAT TAAATTTGGG 
AAGCGGTATA CAATTTACTC TCCGAAAGAT GGACAGCCTT GCATGGATCA TGATAGACAA 
ACTGGAGAGG GTGTTGGACC TCAGGAATAT ACTTTACTCA AATTGAAGGT GCTTGAGCCA 
TACCCATCTA AATTAAGTGG CCTGAAAGGT AAAAATATTT TCTTGGTGGC TGCTACTCTC 
AGACCTGAGA CCATGTTTGG GCAGACAAAT TGTTGGGTTC GTCCTGATAT GAAGTACATT 
GGATTTGAGA CGGTGAATGG TGATATATTC ATCTGTACCC AAAAAGCAGC CAGGAATATG 
TCATACCAGG GCTTTACCAA AGACAATGGC GTGGTGCCTG TTGTTAAGGA ATTAATGGGG 
GAGGAAATTC TTGGTGCATC ACTTTCTGCA CCTTTAACAT CATACAAGGT GATCTATGTT 
CTCCCAATGC TAACTATTAA GGAGGATAAA GGCACTGGTG TGGTTACAAG TGTTCCTTCC 
GACTCCCCTG ATGATATTGC TGCCCTCAGA GACTTGAAGA AAAAGCAAGC CTTACGAGCA 
AAATATGGAA TTAGAGATGA CATGGTCTTG CCATTTGAGC CGGTGCCAGT CATTGAAATC 
CCAGGTTTTG GAAATCTTTC TGCTGTAACC ATTTGTGATG AGTTGAAAAT TCAGAGCCAG 
AATGACCGGG AAAAACTTGC AGAAGCAAAG GAGAAGATAT ATCTAAAAGG ATTTTATGAG 
GGTATCATGT TGGTGGATGG ATTTAAAGGA CAGAAGGTTC AAGATGTAAA GAAGACTATT 
CAGAAAAAGA TGATTGACGC TGGAGATGCA CTTATTTACA TGGAACCAGA GAAACAAGTG 
ATGTCCAGGT CGTCAGATGA ATGTGTTGTG GCTCTGTGTG ACCAGTGGTA CTTGGATTAT 
GGAGAAGAGA ATTGGAAGAA ACAGACATCT CAGTGCTTGA AGAACCTGGA AACATTCTGT 
GAGGAGACCA GGAGGAATTT TGAAGCCACC TTAGGTTGGC TACAAGAACA TGCTTGCTCA 
AGAACTTATG GTCTAGGCAC TCACCTGCCT TGGGATGAGC AGTGGCTGAT TGAATCACTT 
TCTGACTCCA CTATTTACAT GGCATTTTAC ACAGTTGCAC ACCTATTGCA GGGGGGTAAC 
TTGCATGGAC AGGCAGAGTC TCCGCTGGGC ATTAGACCGC AACAGATGAC CAAGGAAGTT 
TGGGATTATG TTTTCTTCAA GGAGGCTCCA TTTCCTAAGA CTCAGATTGC AAAGGAAAAA 
TTAGATCAGT TAAAGCAGGA GTTTGAATTC TGGTATCCTG TTGATCTTCG CGTCTCTGGC 
AAGGATCTTG TTCCAAATCA TCTTTCATAT TACCTTTATA ATCATGTGGC TATGTGGCCG 
GAACAAAGTG ACAAATGGCC TACAGCTGTG AGAGCAAATG GACATCTCCT CCTGAACTCT 
GAGAAGATGT CAAAATCCAC AGGCAACTTC CTCACTTTGA CCCAAGCTAT TGACAAATTT 
TCAGCAGATG GAATGCGTTT GGCTCTGGCT GATGCTGGTG ACACTGTAGA AGATGCCAAC 
TTTGTGGAAG CCATGGCAGA TGCAGGTATT CTCCGTCTGT ACACCTGGGT AGAGTGGGTG 
AAAGAAATGG TTGCCAACTG GGACAGCCTA AGAAGTGGTC CTGCCAGCAC TTTCAATGAT 
AGAGTTTTTG CCAGTGAATT GAATGCAGGA ATTATAAAAA CAGATCAAAA CTATGAAAAG 
ATGATGTTTA AAGAAGCTTT GAAAACAGGG TTTTTTGAGT TTCAGGCCGC AAAAGATAAG 
TACCGTGAAT TGGCTGTGGA AGGGATGCAC AGAGAACTTG TGTTCCGGTT TATTGAAGTT 
CAGACACTTC TCCTCGCTCC ATTCTGTCCA CATTTGTGTG AGCACATCTG GACACTCCTG 
GGAAAGCCTG ACTCAATTAT GAATGCTTCA TGGCCTGTGG CAGGTCCTGT TAATGAAGTT 
TTAATACACT CCTCACAGTA TCTTATGGAA GTAACACATG ACCTTAGACT ACGACTCAAG 
AACTATATGA TGCCAGCTAA AGGGAAGAAG ACTGACAAAC AACCCCTGCA GAAGCCCTCA 
CATTGCACCA TCTATGTGGC AAAGAACTAT CCACCTTGGC AACATACCAC CCTGTCTGTT 
CTACGTAAAC ACTTTGAGGC CAATAACGGA AAACTGCCTG ACAACAAAGT CATTGCTAGT 
GAACTAGGCA GTATGCCAGA ACTGAAGAAA TACATGAAGA AAGTCATGCC ATTTGTTGCC 
ATGATTAAGG AAAATCTGGA GAAGATGGGG CCTCGTATTC TGGATTTGCA ATTAGAATTT 
GATGAAAAGG CTGTGCTTAT GGAGAATATA GTCTATCTGA CTAATTCGCT TGAGCTAGAA 
CACATAGAAG TCAAGTTTGC CTCCGAAGCA GAAGATAAAA TCAGGGAAGA CTGCTGTCCT 
GGGAAACCAC TTAATGTTTT TAGAATAGAA CCTGGTGTGT CCGTTTCTCT GGTGAATCCC 
CAGCCATCCA ATGGCCACTT CTCAACCAAA ATTGAAATCA GGCAAGGAGA TAACTGTGAT 
TCCATAATCA GGCGTTTAAT GAAAATGAAT CGAGGAATTA AAGACCTTTC CAAAGTGAAA 
CTGATGAGAT TTGATGATCC ACTGTTGGGG CCTCGACGAG TTCCTGTCCT GGGAAAGGAG 
TACACCGAGA AGACCCCCAT TTCTGAGCAT GCTGTTTTCA ATGTGGACCT CATGAGCAAG 
AAAATTCATC TGACTGAGAA TGGGATAAGG GTGGATATTG GCGATACAAT AATCTATCTG 
GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304894.1
CDS	338..3706
Translation	MNGRLHLGHTFSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPDEEEEEEETSVKTEDIIIKDKAKGKKSKAAAKAGSSKYQWGIMKSLGLSDEEIVKFSEAEHWLDYFPPLAIQDLKRMGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILGASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIRDDMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDVKKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFCEETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSKSTGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVANWDSLRSGPASTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYRELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVNEVLIHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKLPDNKVIASELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAEDKIREDCCPGKPLNVFRIEPGVSVSLVNPQPSNGHFSTKIEIRQGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDDPLLGPRRVPVLGKEYTEKTPISEHAVFNVDLMSKKIHLTENGIRVDIGDTIIYLVH

Target ORF information:

RefSeq Version	NM_001317965.1
Organism	Homo sapiens(human)
Definition	Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317965.1

ORF Insert Sequence:

ATGAATGGAC GCCTTCATTT GGGACACACG TTTTCTTTAT CCAAATGTGA GTTTGCTGTA 
GGGTACCAGC GATTGAAAGG AAAATGTTGT CTGTTTCCCT TTGGCCTGCA CTGTACTGGA 
ATGCCTATTA AGGCATGTGC TGATAAGTTG AAAAGAGAAA TAGAGCTGTA TGGTTGCCCC 
CCTGATTTTC CAGATGAAGA AGAGGAAGAG GAAGAAACCA GTGTTAAAAC AGAAGATATA 
ATAATTAAGG ATAAAGCTAA AGGAAAAAAG AGTAAAGCTG CTGCTAAAGC TGGATCTTCT 
AAATACCAGT GGGGCATTAT GAAATCCCTT GGCCTGTCTG ATGAAGAGAT AGTAAAATTT 
TCTGAAGCAG AACATTGGCT TGATTATTTC CCGCCACTGG CTATTCAGGA TTTAAAAAGA 
ATGGGTTTGA AGGTAGACTG GCGTCGTTCC TTCATCACCA CTGATGTTAA TCCTTACTAT 
GATTCATTTG TCAGATGGCA ATTTTTAACA TTAAGAGAAA GAAACAAAAT TAAATTTGGG 
AAGCGGTATA CAATTTACTC TCCGAAAGAT GGACAGCCTT GCATGGATCA TGATAGACAA 
ACTGGAGAGG GTGTTGGACC TCAGGAATAT ACTTTACTCA AATTGAAGGT GCTTGAGCCA 
TACCCATCTA AATTAAGTGG CCTGAAAGGT AAAAATATTT TCTTGGTGGC TGCTACTCTC 
AGACCTGAGA CCATGTTTGG GCAGACAAAT TGTTGGGTTC GTCCTGATAT GAAGTACATT 
GGATTTGAGA CGGTGAATGG TGATATATTC ATCTGTACCC AAAAAGCAGC CAGGAATATG 
TCATACCAGG GCTTTACCAA AGACAATGGC GTGGTGCCTG TTGTTAAGGA ATTAATGGGG 
GAGGAAATTC TTGGTGCATC ACTTTCTGCA CCTTTAACAT CATACAAGGT GATCTATGTT 
CTCCCAATGC TAACTATTAA GGAGGATAAA GGCACTGGTG TGGTTACAAG TGTTCCTTCC 
GACTCCCCTG ATGATATTGC TGCCCTCAGA GACTTGAAGA AAAAGCAAGC CTTACGAGCA 
AAATATGGAA TTAGAGATGA CATGGTCTTG CCATTTGAGC CGGTGCCAGT CATTGAAATC 
CCAGGTTTTG GAAATCTTTC TGCTGTAACC ATTTGTGATG AGTTGAAAAT TCAGAGCCAG 
AATGACCGGG AAAAACTTGC AGAAGCAAAG GAGAAGATAT ATCTAAAAGG ATTTTATGAG 
GGTATCATGT TGGTGGATGG ATTTAAAGGA CAGAAGGTTC AAGATGTAAA GAAGACTATT 
CAGAAAAAGA TGATTGACGC TGGAGATGCA CTTATTTACA TGGAACCAGA GAAACAAGTG 
ATGTCCAGGT CGTCAGATGA ATGTGTTGTG GCTCTGTGTG ACCAGTGGTA CTTGGATTAT 
GGAGAAGAGA ATTGGAAGAA ACAGACATCT CAGTGCTTGA AGAACCTGGA AACATTCTGT 
GAGGAGACCA GGAGGAATTT TGAAGCCACC TTAGGTTGGC TACAAGAACA TGCTTGCTCA 
AGAACTTATG GTCTAGGCAC TCACCTGCCT TGGGATGAGC AGTGGCTGAT TGAATCACTT 
TCTGACTCCA CTATTTACAT GGCATTTTAC ACAGTTGCAC ACCTATTGCA GGGGGGTAAC 
TTGCATGGAC AGGCAGAGTC TCCGCTGGGC ATTAGACCGC AACAGATGAC CAAGGAAGTT 
TGGGATTATG TTTTCTTCAA GGAGGCTCCA TTTCCTAAGA CTCAGATTGC AAAGGAAAAA 
TTAGATCAGT TAAAGCAGGA GTTTGAATTC TGGTATCCTG TTGATCTTCG CGTCTCTGGC 
AAGGATCTTG TTCCAAATCA TCTTTCATAT TACCTTTATA ATCATGTGGC TATGTGGCCG 
GAACAAAGTG ACAAATGGCC TACAGCTGTG AGAGCAAATG GACATCTCCT CCTGAACTCT 
GAGAAGATGT CAAAATCCAC AGGCAACTTC CTCACTTTGA CCCAAGCTAT TGACAAATTT 
TCAGCAGATG GAATGCGTTT GGCTCTGGCT GATGCTGGTG ACACTGTAGA AGATGCCAAC 
TTTGTGGAAG CCATGGCAGA TGCAGGTATT CTCCGTCTGT ACACCTGGGT AGAGTGGGTG 
AAAGAAATGG TTGCCAACTG GGACAGCCTA AGAAGTGGTC CTGCCAGCAC TTTCAATGAT 
AGAGTTTTTG CCAGTGAATT GAATGCAGGA ATTATAAAAA CAGATCAAAA CTATGAAAAG 
ATGATGTTTA AAGAAGCTTT GAAAACAGGG TTTTTTGAGT TTCAGGCCGC AAAAGATAAG 
TACCGTGAAT TGGCTGTGGA AGGGATGCAC AGAGAACTTG TGTTCCGGTT TATTGAAGTT 
CAGACACTTC TCCTCGCTCC ATTCTGTCCA CATTTGTGTG AGCACATCTG GACACTCCTG 
GGAAAGCCTG ACTCAATTAT GAATGCTTCA TGGCCTGTGG CAGGTCCTGT TAATGAAGTT 
TTAATACACT CCTCACAGTA TCTTATGGAA GTAACACATG ACCTTAGACT ACGACTCAAG 
AACTATATGA TGCCAGCTAA AGGGAAGAAG ACTGACAAAC AACCCCTGCA GAAGCCCTCA 
CATTGCACCA TCTATGTGGC AAAGAACTAT CCACCTTGGC AACATACCAC CCTGTCTGTT 
CTACGTAAAC ACTTTGAGGC CAATAACGGA AAACTGCCTG ACAACAAAGT CATTGCTAGT 
GAACTAGGCA GTATGCCAGA ACTGAAGAAA TACATGAAGA AAGTCATGCC ATTTGTTGCC 
ATGATTAAGG AAAATCTGGA GAAGATGGGG CCTCGTATTC TGGATTTGCA ATTAGAATTT 
GATGAAAAGG CTGTGCTTAT GGAGAATATA GTCTATCTGA CTAATTCGCT TGAGCTAGAA 
CACATAGAAG TCAAGTTTGC CTCCGAAGCA GAAGATAAAA TCAGGGAAGA CTGCTGTCCT 
GGGAAACCAC TTAATGTTTT TAGAATAGAA CCTGGTGTGT CCGTTTCTCT GGTGAATCCC 
CAGCCATCCA ATGGCCACTT CTCAACCAAA ATTGAAATCA GGCAAGGAGA TAACTGTGAT 
TCCATAATCA GGCGTTTAAT GAAAATGAAT CGAGGAATTA AAGACCTTTC CAAAGTGAAA 
CTGATGAGAT TTGATGATCC ACTGTTGGGG CCTCGACGAG TTCCTGTCCT GGGAAAGGAG 
TACACCGAGA AGACCCCCAT TTCTGAGCAT GCTGTTTTCA ATGTGGACCT CATGAGCAAG 
AAAATTCATC TGACTGAGAA TGGGATAAGG GTGGATATTG GCGATACAAT AATCTATCTG 
GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LARS1 ( XM_011537656.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535958.1 Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant X1, mRNA.

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CloneID	OHu67988
Clone ID Related Accession (Same CDS sequence)	NM_001317965.1 , XM_011537656.3 , XM_011537656.2
Accession Version	XM_011537656.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	leucine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011537656.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAC GCCTTCATTT GGGACACACG TTTTCTTTAT CCAAATGTGA GTTTGCTGTA 
GGGTACCAGC GATTGAAAGG AAAATGTTGT CTGTTTCCCT TTGGCCTGCA CTGTACTGGA 
ATGCCTATTA AGGCATGTGC TGATAAGTTG AAAAGAGAAA TAGAGCTGTA TGGTTGCCCC 
CCTGATTTTC CAGATGAAGA AGAGGAAGAG GAAGAAACCA GTGTTAAAAC AGAAGATATA 
ATAATTAAGG ATAAAGCTAA AGGAAAAAAG AGTAAAGCTG CTGCTAAAGC TGGATCTTCT 
AAATACCAGT GGGGCATTAT GAAATCCCTT GGCCTGTCTG ATGAAGAGAT AGTAAAATTT 
TCTGAAGCAG AACATTGGCT TGATTATTTC CCGCCACTGG CTATTCAGGA TTTAAAAAGA 
ATGGGTTTGA AGGTAGACTG GCGTCGTTCC TTCATCACCA CTGATGTTAA TCCTTACTAT 
GATTCATTTG TCAGATGGCA ATTTTTAACA TTAAGAGAAA GAAACAAAAT TAAATTTGGG 
AAGCGGTATA CAATTTACTC TCCGAAAGAT GGACAGCCTT GCATGGATCA TGATAGACAA 
ACTGGAGAGG GTGTTGGACC TCAGGAATAT ACTTTACTCA AATTGAAGGT GCTTGAGCCA 
TACCCATCTA AATTAAGTGG CCTGAAAGGT AAAAATATTT TCTTGGTGGC TGCTACTCTC 
AGACCTGAGA CCATGTTTGG GCAGACAAAT TGTTGGGTTC GTCCTGATAT GAAGTACATT 
GGATTTGAGA CGGTGAATGG TGATATATTC ATCTGTACCC AAAAAGCAGC CAGGAATATG 
TCATACCAGG GCTTTACCAA AGACAATGGC GTGGTGCCTG TTGTTAAGGA ATTAATGGGG 
GAGGAAATTC TTGGTGCATC ACTTTCTGCA CCTTTAACAT CATACAAGGT GATCTATGTT 
CTCCCAATGC TAACTATTAA GGAGGATAAA GGCACTGGTG TGGTTACAAG TGTTCCTTCC 
GACTCCCCTG ATGATATTGC TGCCCTCAGA GACTTGAAGA AAAAGCAAGC CTTACGAGCA 
AAATATGGAA TTAGAGATGA CATGGTCTTG CCATTTGAGC CGGTGCCAGT CATTGAAATC 
CCAGGTTTTG GAAATCTTTC TGCTGTAACC ATTTGTGATG AGTTGAAAAT TCAGAGCCAG 
AATGACCGGG AAAAACTTGC AGAAGCAAAG GAGAAGATAT ATCTAAAAGG ATTTTATGAG 
GGTATCATGT TGGTGGATGG ATTTAAAGGA CAGAAGGTTC AAGATGTAAA GAAGACTATT 
CAGAAAAAGA TGATTGACGC TGGAGATGCA CTTATTTACA TGGAACCAGA GAAACAAGTG 
ATGTCCAGGT CGTCAGATGA ATGTGTTGTG GCTCTGTGTG ACCAGTGGTA CTTGGATTAT 
GGAGAAGAGA ATTGGAAGAA ACAGACATCT CAGTGCTTGA AGAACCTGGA AACATTCTGT 
GAGGAGACCA GGAGGAATTT TGAAGCCACC TTAGGTTGGC TACAAGAACA TGCTTGCTCA 
AGAACTTATG GTCTAGGCAC TCACCTGCCT TGGGATGAGC AGTGGCTGAT TGAATCACTT 
TCTGACTCCA CTATTTACAT GGCATTTTAC ACAGTTGCAC ACCTATTGCA GGGGGGTAAC 
TTGCATGGAC AGGCAGAGTC TCCGCTGGGC ATTAGACCGC AACAGATGAC CAAGGAAGTT 
TGGGATTATG TTTTCTTCAA GGAGGCTCCA TTTCCTAAGA CTCAGATTGC AAAGGAAAAA 
TTAGATCAGT TAAAGCAGGA GTTTGAATTC TGGTATCCTG TTGATCTTCG CGTCTCTGGC 
AAGGATCTTG TTCCAAATCA TCTTTCATAT TACCTTTATA ATCATGTGGC TATGTGGCCG 
GAACAAAGTG ACAAATGGCC TACAGCTGTG AGAGCAAATG GACATCTCCT CCTGAACTCT 
GAGAAGATGT CAAAATCCAC AGGCAACTTC CTCACTTTGA CCCAAGCTAT TGACAAATTT 
TCAGCAGATG GAATGCGTTT GGCTCTGGCT GATGCTGGTG ACACTGTAGA AGATGCCAAC 
TTTGTGGAAG CCATGGCAGA TGCAGGTATT CTCCGTCTGT ACACCTGGGT AGAGTGGGTG 
AAAGAAATGG TTGCCAACTG GGACAGCCTA AGAAGTGGTC CTGCCAGCAC TTTCAATGAT 
AGAGTTTTTG CCAGTGAATT GAATGCAGGA ATTATAAAAA CAGATCAAAA CTATGAAAAG 
ATGATGTTTA AAGAAGCTTT GAAAACAGGG TTTTTTGAGT TTCAGGCCGC AAAAGATAAG 
TACCGTGAAT TGGCTGTGGA AGGGATGCAC AGAGAACTTG TGTTCCGGTT TATTGAAGTT 
CAGACACTTC TCCTCGCTCC ATTCTGTCCA CATTTGTGTG AGCACATCTG GACACTCCTG 
GGAAAGCCTG ACTCAATTAT GAATGCTTCA TGGCCTGTGG CAGGTCCTGT TAATGAAGTT 
TTAATACACT CCTCACAGTA TCTTATGGAA GTAACACATG ACCTTAGACT ACGACTCAAG 
AACTATATGA TGCCAGCTAA AGGGAAGAAG ACTGACAAAC AACCCCTGCA GAAGCCCTCA 
CATTGCACCA TCTATGTGGC AAAGAACTAT CCACCTTGGC AACATACCAC CCTGTCTGTT 
CTACGTAAAC ACTTTGAGGC CAATAACGGA AAACTGCCTG ACAACAAAGT CATTGCTAGT 
GAACTAGGCA GTATGCCAGA ACTGAAGAAA TACATGAAGA AAGTCATGCC ATTTGTTGCC 
ATGATTAAGG AAAATCTGGA GAAGATGGGG CCTCGTATTC TGGATTTGCA ATTAGAATTT 
GATGAAAAGG CTGTGCTTAT GGAGAATATA GTCTATCTGA CTAATTCGCT TGAGCTAGAA 
CACATAGAAG TCAAGTTTGC CTCCGAAGCA GAAGATAAAA TCAGGGAAGA CTGCTGTCCT 
GGGAAACCAC TTAATGTTTT TAGAATAGAA CCTGGTGTGT CCGTTTCTCT GGTGAATCCC 
CAGCCATCCA ATGGCCACTT CTCAACCAAA ATTGAAATCA GGCAAGGAGA TAACTGTGAT 
TCCATAATCA GGCGTTTAAT GAAAATGAAT CGAGGAATTA AAGACCTTTC CAAAGTGAAA 
CTGATGAGAT TTGATGATCC ACTGTTGGGG CCTCGACGAG TTCCTGTCCT GGGAAAGGAG 
TACACCGAGA AGACCCCCAT TTCTGAGCAT GCTGTTTTCA ATGTGGACCT CATGAGCAAG 
AAAATTCATC TGACTGAGAA TGGGATAAGG GTGGATATTG GCGATACAAT AATCTATCTG 
GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535958.1
CDS	267..3635
Translation	MNGRLHLGHTFSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPDEEEEEEETSVKTEDIIIKDKAKGKKSKAAAKAGSSKYQWGIMKSLGLSDEEIVKFSEAEHWLDYFPPLAIQDLKRMGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILGASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIRDDMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDVKKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFCEETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSKSTGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVANWDSLRSGPASTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYRELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVNEVLIHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKLPDNKVIASELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAEDKIREDCCPGKPLNVFRIEPGVSVSLVNPQPSNGHFSTKIEIRQGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDDPLLGPRRVPVLGKEYTEKTPISEHAVFNVDLMSKKIHLTENGIRVDIGDTIIYLVH

Target ORF information:

RefSeq Version	XM_011537656.3
Organism	Homo sapiens(human)
Definition	Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537656.3

ORF Insert Sequence:

ATGAATGGAC GCCTTCATTT GGGACACACG TTTTCTTTAT CCAAATGTGA GTTTGCTGTA 
GGGTACCAGC GATTGAAAGG AAAATGTTGT CTGTTTCCCT TTGGCCTGCA CTGTACTGGA 
ATGCCTATTA AGGCATGTGC TGATAAGTTG AAAAGAGAAA TAGAGCTGTA TGGTTGCCCC 
CCTGATTTTC CAGATGAAGA AGAGGAAGAG GAAGAAACCA GTGTTAAAAC AGAAGATATA 
ATAATTAAGG ATAAAGCTAA AGGAAAAAAG AGTAAAGCTG CTGCTAAAGC TGGATCTTCT 
AAATACCAGT GGGGCATTAT GAAATCCCTT GGCCTGTCTG ATGAAGAGAT AGTAAAATTT 
TCTGAAGCAG AACATTGGCT TGATTATTTC CCGCCACTGG CTATTCAGGA TTTAAAAAGA 
ATGGGTTTGA AGGTAGACTG GCGTCGTTCC TTCATCACCA CTGATGTTAA TCCTTACTAT 
GATTCATTTG TCAGATGGCA ATTTTTAACA TTAAGAGAAA GAAACAAAAT TAAATTTGGG 
AAGCGGTATA CAATTTACTC TCCGAAAGAT GGACAGCCTT GCATGGATCA TGATAGACAA 
ACTGGAGAGG GTGTTGGACC TCAGGAATAT ACTTTACTCA AATTGAAGGT GCTTGAGCCA 
TACCCATCTA AATTAAGTGG CCTGAAAGGT AAAAATATTT TCTTGGTGGC TGCTACTCTC 
AGACCTGAGA CCATGTTTGG GCAGACAAAT TGTTGGGTTC GTCCTGATAT GAAGTACATT 
GGATTTGAGA CGGTGAATGG TGATATATTC ATCTGTACCC AAAAAGCAGC CAGGAATATG 
TCATACCAGG GCTTTACCAA AGACAATGGC GTGGTGCCTG TTGTTAAGGA ATTAATGGGG 
GAGGAAATTC TTGGTGCATC ACTTTCTGCA CCTTTAACAT CATACAAGGT GATCTATGTT 
CTCCCAATGC TAACTATTAA GGAGGATAAA GGCACTGGTG TGGTTACAAG TGTTCCTTCC 
GACTCCCCTG ATGATATTGC TGCCCTCAGA GACTTGAAGA AAAAGCAAGC CTTACGAGCA 
AAATATGGAA TTAGAGATGA CATGGTCTTG CCATTTGAGC CGGTGCCAGT CATTGAAATC 
CCAGGTTTTG GAAATCTTTC TGCTGTAACC ATTTGTGATG AGTTGAAAAT TCAGAGCCAG 
AATGACCGGG AAAAACTTGC AGAAGCAAAG GAGAAGATAT ATCTAAAAGG ATTTTATGAG 
GGTATCATGT TGGTGGATGG ATTTAAAGGA CAGAAGGTTC AAGATGTAAA GAAGACTATT 
CAGAAAAAGA TGATTGACGC TGGAGATGCA CTTATTTACA TGGAACCAGA GAAACAAGTG 
ATGTCCAGGT CGTCAGATGA ATGTGTTGTG GCTCTGTGTG ACCAGTGGTA CTTGGATTAT 
GGAGAAGAGA ATTGGAAGAA ACAGACATCT CAGTGCTTGA AGAACCTGGA AACATTCTGT 
GAGGAGACCA GGAGGAATTT TGAAGCCACC TTAGGTTGGC TACAAGAACA TGCTTGCTCA 
AGAACTTATG GTCTAGGCAC TCACCTGCCT TGGGATGAGC AGTGGCTGAT TGAATCACTT 
TCTGACTCCA CTATTTACAT GGCATTTTAC ACAGTTGCAC ACCTATTGCA GGGGGGTAAC 
TTGCATGGAC AGGCAGAGTC TCCGCTGGGC ATTAGACCGC AACAGATGAC CAAGGAAGTT 
TGGGATTATG TTTTCTTCAA GGAGGCTCCA TTTCCTAAGA CTCAGATTGC AAAGGAAAAA 
TTAGATCAGT TAAAGCAGGA GTTTGAATTC TGGTATCCTG TTGATCTTCG CGTCTCTGGC 
AAGGATCTTG TTCCAAATCA TCTTTCATAT TACCTTTATA ATCATGTGGC TATGTGGCCG 
GAACAAAGTG ACAAATGGCC TACAGCTGTG AGAGCAAATG GACATCTCCT CCTGAACTCT 
GAGAAGATGT CAAAATCCAC AGGCAACTTC CTCACTTTGA CCCAAGCTAT TGACAAATTT 
TCAGCAGATG GAATGCGTTT GGCTCTGGCT GATGCTGGTG ACACTGTAGA AGATGCCAAC 
TTTGTGGAAG CCATGGCAGA TGCAGGTATT CTCCGTCTGT ACACCTGGGT AGAGTGGGTG 
AAAGAAATGG TTGCCAACTG GGACAGCCTA AGAAGTGGTC CTGCCAGCAC TTTCAATGAT 
AGAGTTTTTG CCAGTGAATT GAATGCAGGA ATTATAAAAA CAGATCAAAA CTATGAAAAG 
ATGATGTTTA AAGAAGCTTT GAAAACAGGG TTTTTTGAGT TTCAGGCCGC AAAAGATAAG 
TACCGTGAAT TGGCTGTGGA AGGGATGCAC AGAGAACTTG TGTTCCGGTT TATTGAAGTT 
CAGACACTTC TCCTCGCTCC ATTCTGTCCA CATTTGTGTG AGCACATCTG GACACTCCTG 
GGAAAGCCTG ACTCAATTAT GAATGCTTCA TGGCCTGTGG CAGGTCCTGT TAATGAAGTT 
TTAATACACT CCTCACAGTA TCTTATGGAA GTAACACATG ACCTTAGACT ACGACTCAAG 
AACTATATGA TGCCAGCTAA AGGGAAGAAG ACTGACAAAC AACCCCTGCA GAAGCCCTCA 
CATTGCACCA TCTATGTGGC AAAGAACTAT CCACCTTGGC AACATACCAC CCTGTCTGTT 
CTACGTAAAC ACTTTGAGGC CAATAACGGA AAACTGCCTG ACAACAAAGT CATTGCTAGT 
GAACTAGGCA GTATGCCAGA ACTGAAGAAA TACATGAAGA AAGTCATGCC ATTTGTTGCC 
ATGATTAAGG AAAATCTGGA GAAGATGGGG CCTCGTATTC TGGATTTGCA ATTAGAATTT 
GATGAAAAGG CTGTGCTTAT GGAGAATATA GTCTATCTGA CTAATTCGCT TGAGCTAGAA 
CACATAGAAG TCAAGTTTGC CTCCGAAGCA GAAGATAAAA TCAGGGAAGA CTGCTGTCCT 
GGGAAACCAC TTAATGTTTT TAGAATAGAA CCTGGTGTGT CCGTTTCTCT GGTGAATCCC 
CAGCCATCCA ATGGCCACTT CTCAACCAAA ATTGAAATCA GGCAAGGAGA TAACTGTGAT 
TCCATAATCA GGCGTTTAAT GAAAATGAAT CGAGGAATTA AAGACCTTTC CAAAGTGAAA 
CTGATGAGAT TTGATGATCC ACTGTTGGGG CCTCGACGAG TTCCTGTCCT GGGAAAGGAG 
TACACCGAGA AGACCCCCAT TTCTGAGCAT GCTGTTTTCA ATGTGGACCT CATGAGCAAG 
AAAATTCATC TGACTGAGAA TGGGATAAGG GTGGATATTG GCGATACAAT AATCTATCTG 
GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LARS1 ( XM_011537656.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535958.1 Homo sapiens leucyl-tRNA synthetase (LARS), transcript variant X1, mRNA.

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CloneID	OHu67988
Clone ID Related Accession (Same CDS sequence)	NM_001317965.1 , XM_011537656.3 , XM_011537656.2
Accession Version	XM_011537656.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	leucine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAC GCCTTCATTT GGGACACACG TTTTCTTTAT CCAAATGTGA GTTTGCTGTA 
GGGTACCAGC GATTGAAAGG AAAATGTTGT CTGTTTCCCT TTGGCCTGCA CTGTACTGGA 
ATGCCTATTA AGGCATGTGC TGATAAGTTG AAAAGAGAAA TAGAGCTGTA TGGTTGCCCC 
CCTGATTTTC CAGATGAAGA AGAGGAAGAG GAAGAAACCA GTGTTAAAAC AGAAGATATA 
ATAATTAAGG ATAAAGCTAA AGGAAAAAAG AGTAAAGCTG CTGCTAAAGC TGGATCTTCT 
AAATACCAGT GGGGCATTAT GAAATCCCTT GGCCTGTCTG ATGAAGAGAT AGTAAAATTT 
TCTGAAGCAG AACATTGGCT TGATTATTTC CCGCCACTGG CTATTCAGGA TTTAAAAAGA 
ATGGGTTTGA AGGTAGACTG GCGTCGTTCC TTCATCACCA CTGATGTTAA TCCTTACTAT 
GATTCATTTG TCAGATGGCA ATTTTTAACA TTAAGAGAAA GAAACAAAAT TAAATTTGGG 
AAGCGGTATA CAATTTACTC TCCGAAAGAT GGACAGCCTT GCATGGATCA TGATAGACAA 
ACTGGAGAGG GTGTTGGACC TCAGGAATAT ACTTTACTCA AATTGAAGGT GCTTGAGCCA 
TACCCATCTA AATTAAGTGG CCTGAAAGGT AAAAATATTT TCTTGGTGGC TGCTACTCTC 
AGACCTGAGA CCATGTTTGG GCAGACAAAT TGTTGGGTTC GTCCTGATAT GAAGTACATT 
GGATTTGAGA CGGTGAATGG TGATATATTC ATCTGTACCC AAAAAGCAGC CAGGAATATG 
TCATACCAGG GCTTTACCAA AGACAATGGC GTGGTGCCTG TTGTTAAGGA ATTAATGGGG 
GAGGAAATTC TTGGTGCATC ACTTTCTGCA CCTTTAACAT CATACAAGGT GATCTATGTT 
CTCCCAATGC TAACTATTAA GGAGGATAAA GGCACTGGTG TGGTTACAAG TGTTCCTTCC 
GACTCCCCTG ATGATATTGC TGCCCTCAGA GACTTGAAGA AAAAGCAAGC CTTACGAGCA 
AAATATGGAA TTAGAGATGA CATGGTCTTG CCATTTGAGC CGGTGCCAGT CATTGAAATC 
CCAGGTTTTG GAAATCTTTC TGCTGTAACC ATTTGTGATG AGTTGAAAAT TCAGAGCCAG 
AATGACCGGG AAAAACTTGC AGAAGCAAAG GAGAAGATAT ATCTAAAAGG ATTTTATGAG 
GGTATCATGT TGGTGGATGG ATTTAAAGGA CAGAAGGTTC AAGATGTAAA GAAGACTATT 
CAGAAAAAGA TGATTGACGC TGGAGATGCA CTTATTTACA TGGAACCAGA GAAACAAGTG 
ATGTCCAGGT CGTCAGATGA ATGTGTTGTG GCTCTGTGTG ACCAGTGGTA CTTGGATTAT 
GGAGAAGAGA ATTGGAAGAA ACAGACATCT CAGTGCTTGA AGAACCTGGA AACATTCTGT 
GAGGAGACCA GGAGGAATTT TGAAGCCACC TTAGGTTGGC TACAAGAACA TGCTTGCTCA 
AGAACTTATG GTCTAGGCAC TCACCTGCCT TGGGATGAGC AGTGGCTGAT TGAATCACTT 
TCTGACTCCA CTATTTACAT GGCATTTTAC ACAGTTGCAC ACCTATTGCA GGGGGGTAAC 
TTGCATGGAC AGGCAGAGTC TCCGCTGGGC ATTAGACCGC AACAGATGAC CAAGGAAGTT 
TGGGATTATG TTTTCTTCAA GGAGGCTCCA TTTCCTAAGA CTCAGATTGC AAAGGAAAAA 
TTAGATCAGT TAAAGCAGGA GTTTGAATTC TGGTATCCTG TTGATCTTCG CGTCTCTGGC 
AAGGATCTTG TTCCAAATCA TCTTTCATAT TACCTTTATA ATCATGTGGC TATGTGGCCG 
GAACAAAGTG ACAAATGGCC TACAGCTGTG AGAGCAAATG GACATCTCCT CCTGAACTCT 
GAGAAGATGT CAAAATCCAC AGGCAACTTC CTCACTTTGA CCCAAGCTAT TGACAAATTT 
TCAGCAGATG GAATGCGTTT GGCTCTGGCT GATGCTGGTG ACACTGTAGA AGATGCCAAC 
TTTGTGGAAG CCATGGCAGA TGCAGGTATT CTCCGTCTGT ACACCTGGGT AGAGTGGGTG 
AAAGAAATGG TTGCCAACTG GGACAGCCTA AGAAGTGGTC CTGCCAGCAC TTTCAATGAT 
AGAGTTTTTG CCAGTGAATT GAATGCAGGA ATTATAAAAA CAGATCAAAA CTATGAAAAG 
ATGATGTTTA AAGAAGCTTT GAAAACAGGG TTTTTTGAGT TTCAGGCCGC AAAAGATAAG 
TACCGTGAAT TGGCTGTGGA AGGGATGCAC AGAGAACTTG TGTTCCGGTT TATTGAAGTT 
CAGACACTTC TCCTCGCTCC ATTCTGTCCA CATTTGTGTG AGCACATCTG GACACTCCTG 
GGAAAGCCTG ACTCAATTAT GAATGCTTCA TGGCCTGTGG CAGGTCCTGT TAATGAAGTT 
TTAATACACT CCTCACAGTA TCTTATGGAA GTAACACATG ACCTTAGACT ACGACTCAAG 
AACTATATGA TGCCAGCTAA AGGGAAGAAG ACTGACAAAC AACCCCTGCA GAAGCCCTCA 
CATTGCACCA TCTATGTGGC AAAGAACTAT CCACCTTGGC AACATACCAC CCTGTCTGTT 
CTACGTAAAC ACTTTGAGGC CAATAACGGA AAACTGCCTG ACAACAAAGT CATTGCTAGT 
GAACTAGGCA GTATGCCAGA ACTGAAGAAA TACATGAAGA AAGTCATGCC ATTTGTTGCC 
ATGATTAAGG AAAATCTGGA GAAGATGGGG CCTCGTATTC TGGATTTGCA ATTAGAATTT 
GATGAAAAGG CTGTGCTTAT GGAGAATATA GTCTATCTGA CTAATTCGCT TGAGCTAGAA 
CACATAGAAG TCAAGTTTGC CTCCGAAGCA GAAGATAAAA TCAGGGAAGA CTGCTGTCCT 
GGGAAACCAC TTAATGTTTT TAGAATAGAA CCTGGTGTGT CCGTTTCTCT GGTGAATCCC 
CAGCCATCCA ATGGCCACTT CTCAACCAAA ATTGAAATCA GGCAAGGAGA TAACTGTGAT 
TCCATAATCA GGCGTTTAAT GAAAATGAAT CGAGGAATTA AAGACCTTTC CAAAGTGAAA 
CTGATGAGAT TTGATGATCC ACTGTTGGGG CCTCGACGAG TTCCTGTCCT GGGAAAGGAG 
TACACCGAGA AGACCCCCAT TTCTGAGCAT GCTGTTTTCA ATGTGGACCT CATGAGCAAG 
AAAATTCATC TGACTGAGAA TGGGATAAGG GTGGATATTG GCGATACAAT AATCTATCTG 
GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535958.1
CDS	323..3691
Translation	MNGRLHLGHTFSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPDEEEEEEETSVKTEDIIIKDKAKGKKSKAAAKAGSSKYQWGIMKSLGLSDEEIVKFSEAEHWLDYFPPLAIQDLKRMGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILGASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIRDDMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDVKKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFCEETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSKSTGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVANWDSLRSGPASTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYRELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVNEVLIHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKLPDNKVIASELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAEDKIREDCCPGKPLNVFRIEPGVSVSLVNPQPSNGHFSTKIEIRQGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDDPLLGPRRVPVLGKEYTEKTPISEHAVFNVDLMSKKIHLTENGIRVDIGDTIIYLVH

Target ORF information:

RefSeq Version	XM_011537656.2
Organism	Homo sapiens(human)
Definition	Homo sapiens leucyl-tRNA synthetase (LARS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537656.2

ORF Insert Sequence:

ATGAATGGAC GCCTTCATTT GGGACACACG TTTTCTTTAT CCAAATGTGA GTTTGCTGTA 
GGGTACCAGC GATTGAAAGG AAAATGTTGT CTGTTTCCCT TTGGCCTGCA CTGTACTGGA 
ATGCCTATTA AGGCATGTGC TGATAAGTTG AAAAGAGAAA TAGAGCTGTA TGGTTGCCCC 
CCTGATTTTC CAGATGAAGA AGAGGAAGAG GAAGAAACCA GTGTTAAAAC AGAAGATATA 
ATAATTAAGG ATAAAGCTAA AGGAAAAAAG AGTAAAGCTG CTGCTAAAGC TGGATCTTCT 
AAATACCAGT GGGGCATTAT GAAATCCCTT GGCCTGTCTG ATGAAGAGAT AGTAAAATTT 
TCTGAAGCAG AACATTGGCT TGATTATTTC CCGCCACTGG CTATTCAGGA TTTAAAAAGA 
ATGGGTTTGA AGGTAGACTG GCGTCGTTCC TTCATCACCA CTGATGTTAA TCCTTACTAT 
GATTCATTTG TCAGATGGCA ATTTTTAACA TTAAGAGAAA GAAACAAAAT TAAATTTGGG 
AAGCGGTATA CAATTTACTC TCCGAAAGAT GGACAGCCTT GCATGGATCA TGATAGACAA 
ACTGGAGAGG GTGTTGGACC TCAGGAATAT ACTTTACTCA AATTGAAGGT GCTTGAGCCA 
TACCCATCTA AATTAAGTGG CCTGAAAGGT AAAAATATTT TCTTGGTGGC TGCTACTCTC 
AGACCTGAGA CCATGTTTGG GCAGACAAAT TGTTGGGTTC GTCCTGATAT GAAGTACATT 
GGATTTGAGA CGGTGAATGG TGATATATTC ATCTGTACCC AAAAAGCAGC CAGGAATATG 
TCATACCAGG GCTTTACCAA AGACAATGGC GTGGTGCCTG TTGTTAAGGA ATTAATGGGG 
GAGGAAATTC TTGGTGCATC ACTTTCTGCA CCTTTAACAT CATACAAGGT GATCTATGTT 
CTCCCAATGC TAACTATTAA GGAGGATAAA GGCACTGGTG TGGTTACAAG TGTTCCTTCC 
GACTCCCCTG ATGATATTGC TGCCCTCAGA GACTTGAAGA AAAAGCAAGC CTTACGAGCA 
AAATATGGAA TTAGAGATGA CATGGTCTTG CCATTTGAGC CGGTGCCAGT CATTGAAATC 
CCAGGTTTTG GAAATCTTTC TGCTGTAACC ATTTGTGATG AGTTGAAAAT TCAGAGCCAG 
AATGACCGGG AAAAACTTGC AGAAGCAAAG GAGAAGATAT ATCTAAAAGG ATTTTATGAG 
GGTATCATGT TGGTGGATGG ATTTAAAGGA CAGAAGGTTC AAGATGTAAA GAAGACTATT 
CAGAAAAAGA TGATTGACGC TGGAGATGCA CTTATTTACA TGGAACCAGA GAAACAAGTG 
ATGTCCAGGT CGTCAGATGA ATGTGTTGTG GCTCTGTGTG ACCAGTGGTA CTTGGATTAT 
GGAGAAGAGA ATTGGAAGAA ACAGACATCT CAGTGCTTGA AGAACCTGGA AACATTCTGT 
GAGGAGACCA GGAGGAATTT TGAAGCCACC TTAGGTTGGC TACAAGAACA TGCTTGCTCA 
AGAACTTATG GTCTAGGCAC TCACCTGCCT TGGGATGAGC AGTGGCTGAT TGAATCACTT 
TCTGACTCCA CTATTTACAT GGCATTTTAC ACAGTTGCAC ACCTATTGCA GGGGGGTAAC 
TTGCATGGAC AGGCAGAGTC TCCGCTGGGC ATTAGACCGC AACAGATGAC CAAGGAAGTT 
TGGGATTATG TTTTCTTCAA GGAGGCTCCA TTTCCTAAGA CTCAGATTGC AAAGGAAAAA 
TTAGATCAGT TAAAGCAGGA GTTTGAATTC TGGTATCCTG TTGATCTTCG CGTCTCTGGC 
AAGGATCTTG TTCCAAATCA TCTTTCATAT TACCTTTATA ATCATGTGGC TATGTGGCCG 
GAACAAAGTG ACAAATGGCC TACAGCTGTG AGAGCAAATG GACATCTCCT CCTGAACTCT 
GAGAAGATGT CAAAATCCAC AGGCAACTTC CTCACTTTGA CCCAAGCTAT TGACAAATTT 
TCAGCAGATG GAATGCGTTT GGCTCTGGCT GATGCTGGTG ACACTGTAGA AGATGCCAAC 
TTTGTGGAAG CCATGGCAGA TGCAGGTATT CTCCGTCTGT ACACCTGGGT AGAGTGGGTG 
AAAGAAATGG TTGCCAACTG GGACAGCCTA AGAAGTGGTC CTGCCAGCAC TTTCAATGAT 
AGAGTTTTTG CCAGTGAATT GAATGCAGGA ATTATAAAAA CAGATCAAAA CTATGAAAAG 
ATGATGTTTA AAGAAGCTTT GAAAACAGGG TTTTTTGAGT TTCAGGCCGC AAAAGATAAG 
TACCGTGAAT TGGCTGTGGA AGGGATGCAC AGAGAACTTG TGTTCCGGTT TATTGAAGTT 
CAGACACTTC TCCTCGCTCC ATTCTGTCCA CATTTGTGTG AGCACATCTG GACACTCCTG 
GGAAAGCCTG ACTCAATTAT GAATGCTTCA TGGCCTGTGG CAGGTCCTGT TAATGAAGTT 
TTAATACACT CCTCACAGTA TCTTATGGAA GTAACACATG ACCTTAGACT ACGACTCAAG 
AACTATATGA TGCCAGCTAA AGGGAAGAAG ACTGACAAAC AACCCCTGCA GAAGCCCTCA 
CATTGCACCA TCTATGTGGC AAAGAACTAT CCACCTTGGC AACATACCAC CCTGTCTGTT 
CTACGTAAAC ACTTTGAGGC CAATAACGGA AAACTGCCTG ACAACAAAGT CATTGCTAGT 
GAACTAGGCA GTATGCCAGA ACTGAAGAAA TACATGAAGA AAGTCATGCC ATTTGTTGCC 
ATGATTAAGG AAAATCTGGA GAAGATGGGG CCTCGTATTC TGGATTTGCA ATTAGAATTT 
GATGAAAAGG CTGTGCTTAT GGAGAATATA GTCTATCTGA CTAATTCGCT TGAGCTAGAA 
CACATAGAAG TCAAGTTTGC CTCCGAAGCA GAAGATAAAA TCAGGGAAGA CTGCTGTCCT 
GGGAAACCAC TTAATGTTTT TAGAATAGAA CCTGGTGTGT CCGTTTCTCT GGTGAATCCC 
CAGCCATCCA ATGGCCACTT CTCAACCAAA ATTGAAATCA GGCAAGGAGA TAACTGTGAT 
TCCATAATCA GGCGTTTAAT GAAAATGAAT CGAGGAATTA AAGACCTTTC CAAAGTGAAA 
CTGATGAGAT TTGATGATCC ACTGTTGGGG CCTCGACGAG TTCCTGTCCT GGGAAAGGAG 
TACACCGAGA AGACCCCCAT TTCTGAGCAT GCTGTTTTCA ATGTGGACCT CATGAGCAAG 
AAAATTCATC TGACTGAGAA TGGGATAAGG GTGGATATTG GCGATACAAT AATCTATCTG 
GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LARS1 ( NM_016460.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057544.2 Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 2, mRNA.

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CloneID	OHu101697
Clone ID Related Accession (Same CDS sequence)	NM_016460.3
Accession Version	NM_016460.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	leucine--tRNA ligase, cytoplasmic isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB091795.1, AB037773.1, AK293750.1, AC091887.3 and CK902545.1. On Dec 17, 2015 this sequence version replaced NM_016460.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.170620.1, AK293750.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG 
ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT 
TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGGCATGTG CTGATAAGTT GAAAAGAGAA 
ATAGAGCTGT ATGGTTGCCC CCCTGATTTT CCAGATGAAG AAGAGGAAGA GGAAGAAACC 
AGTGTTAAAA CAGAAGATAT AATAATTAAG GATAAAGCTA AAGGAAAAAA GAGTAAAGCT 
GCTGCTAAAG CTGGATCTTC TAAATACCAG TGGGGCATTA TGAAATCCCT TGGCCTGTCT 
GATGAAGAGA TAGTAAAATT TTCTGAAGCA GAACATTGGC TTGATTATTT CCCGCCACTG 
GCTATTCAGG ATTTAAAAAG AATGGGTTTG AAGGTAGACT GGCGTCGTTC CTTCATCACC 
ACTGATGTTA ATCCTTACTA TGATTCATTT GTCAGATGGC AATTTTTAAC ATTAAGAGAA 
AGAAACAAAA TTAAATTTGG GAAGCGGTAT ACAATTTACT CTCCGAAAGA TGGACAGCCT 
TGCATGGATC ATGATAGACA AACTGGAGAG GGTGTTGGAC CTCAGGAATA TACTTTACTC 
AAATTGAAGG TGCTTGAGCC ATACCCATCT AAATTAAGTG GCCTGAAAGG TAAAAATATT 
TTCTTGGTGG CTGCTACTCT CAGACCTGAG ACCATGTTTG GGCAGACAAA TTGTTGGGTT 
CGTCCTGATA TGAAGTACAT TGGATTTGAG ACGGTGAATG GTGATATATT CATCTGTACC 
CAAAAAGCAG CCAGGAATAT GTCATACCAG GGCTTTACCA AAGACAATGG CGTGGTGCCT 
GTTGTTAAGG AATTAATGGG GGAGGAAATT CTTGGTGCAT CACTTTCTGC ACCTTTAACA 
TCATACAAGG TGATCTATGT TCTCCCAATG CTAACTATTA AGGAGGATAA AGGCACTGGT 
GTGGTTACAA GTGTTCCTTC CGACTCCCCT GATGATATTG CTGCCCTCAG AGACTTGAAG 
AAAAAGCAAG CCTTACGAGC AAAATATGGA ATTAGAGATG ACATGGTCTT GCCATTTGAG 
CCGGTGCCAG TCATTGAAAT CCCAGGTTTT GGAAATCTTT CTGCTGTAAC CATTTGTGAT 
GAGTTGAAAA TTCAGAGCCA GAATGACCGG GAAAAACTTG CAGAAGCAAA GGAGAAGATA 
TATCTAAAAG GATTTTATGA GGGTATCATG TTGGTGGATG GATTTAAAGG ACAGAAGGTT 
CAAGATGTAA AGAAGACTAT TCAGAAAAAG ATGATTGACG CTGGAGATGC ACTTATTTAC 
ATGGAACCAG AGAAACAAGT GATGTCCAGG TCGTCAGATG AATGTGTTGT GGCTCTGTGT 
GACCAGTGGT ACTTGGATTA TGGAGAAGAG AATTGGAAGA AACAGACATC TCAGTGCTTG 
AAGAACCTGG AAACATTCTG TGAGGAGACC AGGAGGAATT TTGAAGCCAC CTTAGGTTGG 
CTACAAGAAC ATGCTTGCTC AAGAACTTAT GGTCTAGGCA CTCACCTGCC TTGGGATGAG 
CAGTGGCTGA TTGAATCACT TTCTGACTCC ACTATTTACA TGGCATTTTA CACAGTTGCA 
CACCTATTGC AGGGGGGTAA CTTGCATGGA CAGGCAGAGT CTCCGCTGGG CATTAGACCG 
CAACAGATGA CCAAGGAAGT TTGGGATTAT GTTTTCTTCA AGGAGGCTCC ATTTCCTAAG 
ACTCAGATTG CAAAGGAAAA ATTAGATCAG TTAAAGCAGG AGTTTGAATT CTGGTATCCT 
GTTGATCTTC GCGTCTCTGG CAAGGATCTT GTTCCAAATC ATCTTTCATA TTACCTTTAT 
AATCATGTGG CTATGTGGCC GGAACAAAGT GACAAATGGC CTACAGCTGT GAGAGCAAAT 
GGACATCTCC TCCTGAACTC TGAGAAGATG TCAAAATCCA CAGGCAACTT CCTCACTTTG 
ACCCAAGCTA TTGACAAATT TTCAGCAGAT GGAATGCGTT TGGCTCTGGC TGATGCTGGT 
GACACTGTAG AAGATGCCAA CTTTGTGGAA GCCATGGCAG ATGCAGGTAT TCTCCGTCTG 
TACACCTGGG TAGAGTGGGT GAAAGAAATG GTTGCCAACT GGGACAGCCT AAGAAGTGGT 
CCTGCCAGCA CTTTCAATGA TAGAGTTTTT GCCAGTGAAT TGAATGCAGG AATTATAAAA 
ACAGATCAAA ACTATGAAAA GATGATGTTT AAAGAAGCTT TGAAAACAGG GTTTTTTGAG 
TTTCAGGCCG CAAAAGATAA GTACCGTGAA TTGGCTGTGG AAGGGATGCA CAGAGAACTT 
GTGTTCCGGT TTATTGAAGT TCAGACACTT CTCCTCGCTC CATTCTGTCC ACATTTGTGT 
GAGCACATCT GGACACTCCT GGGAAAGCCT GACTCAATTA TGAATGCTTC ATGGCCTGTG 
GCAGGTCCTG TTAATGAAGT TTTAATACAC TCCTCACAGT ATCTTATGGA AGTAACACAT 
GACCTTAGAC TACGACTCAA GAACTATATG ATGCCAGCTA AAGGGAAGAA GACTGACAAA 
CAACCCCTGC AGAAGCCCTC ACATTGCACC ATCTATGTGG CAAAGAACTA TCCACCTTGG 
CAACATACCA CCCTGTCTGT TCTACGTAAA CACTTTGAGG CCAATAACGG AAAACTGCCT 
GACAACAAAG TCATTGCTAG TGAACTAGGC AGTATGCCAG AACTGAAGAA ATACATGAAG 
AAAGTCATGC CATTTGTTGC CATGATTAAG GAAAATCTGG AGAAGATGGG GCCTCGTATT 
CTGGATTTGC AATTAGAATT TGATGAAAAG GCTGTGCTTA TGGAGAATAT AGTCTATCTG 
ACTAATTCGC TTGAGCTAGA ACACATAGAA GTCAAGTTTG CCTCCGAAGC AGAAGATAAA 
ATCAGGGAAG ACTGCTGTCC TGGGAAACCA CTTAATGTTT TTAGAATAGA ACCTGGTGTG 
TCCGTTTCTC TGGTGAATCC CCAGCCATCC AATGGCCACT TCTCAACCAA AATTGAAATC 
AGGCAAGGAG ATAACTGTGA TTCCATAATC AGGCGTTTAA TGAAAATGAA TCGAGGAATT 
AAAGACCTTT CCAAAGTGAA ACTGATGAGA TTTGATGATC CACTGTTGGG GCCTCGACGA 
GTTCCTGTCC TGGGAAAGGA GTACACCGAG AAGACCCCCA TTTCTGAGCA TGCTGTTTTC 
AATGTGGACC TCATGAGCAA GAAAATTCAT CTGACTGAGA ATGGGATAAG GGTGGATATT 
GGCGATACAA TAATCTATCT GGTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057544.2
CDS	295..3744
Translation	MAERKGTAKVDFLKKIEKEIQQKWDTERVFEVNASNLEKQTSKGKYFVTFPYPYMNGRLHLGHTFSLSKCEACADKLKREIELYGCPPDFPDEEEEEEETSVKTEDIIIKDKAKGKKSKAAAKAGSSKYQWGIMKSLGLSDEEIVKFSEAEHWLDYFPPLAIQDLKRMGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILGASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIRDDMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDVKKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFCEETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSKSTGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVANWDSLRSGPASTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYRELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVNEVLIHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKLPDNKVIASELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAEDKIREDCCPGKPLNVFRIEPGVSVSLVNPQPSNGHFSTKIEIRQGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDDPLLGPRRVPVLGKEYTEKTPISEHAVFNVDLMSKKIHLTENGIRVDIGDTIIYLVH

Target ORF information:

RefSeq Version	NM_016460.3
Organism	Homo sapiens(human)
Definition	Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016460.3

ORF Insert Sequence:

ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG 
ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT 
TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGGCATGTG CTGATAAGTT GAAAAGAGAA 
ATAGAGCTGT ATGGTTGCCC CCCTGATTTT CCAGATGAAG AAGAGGAAGA GGAAGAAACC 
AGTGTTAAAA CAGAAGATAT AATAATTAAG GATAAAGCTA AAGGAAAAAA GAGTAAAGCT 
GCTGCTAAAG CTGGATCTTC TAAATACCAG TGGGGCATTA TGAAATCCCT TGGCCTGTCT 
GATGAAGAGA TAGTAAAATT TTCTGAAGCA GAACATTGGC TTGATTATTT CCCGCCACTG 
GCTATTCAGG ATTTAAAAAG AATGGGTTTG AAGGTAGACT GGCGTCGTTC CTTCATCACC 
ACTGATGTTA ATCCTTACTA TGATTCATTT GTCAGATGGC AATTTTTAAC ATTAAGAGAA 
AGAAACAAAA TTAAATTTGG GAAGCGGTAT ACAATTTACT CTCCGAAAGA TGGACAGCCT 
TGCATGGATC ATGATAGACA AACTGGAGAG GGTGTTGGAC CTCAGGAATA TACTTTACTC 
AAATTGAAGG TGCTTGAGCC ATACCCATCT AAATTAAGTG GCCTGAAAGG TAAAAATATT 
TTCTTGGTGG CTGCTACTCT CAGACCTGAG ACCATGTTTG GGCAGACAAA TTGTTGGGTT 
CGTCCTGATA TGAAGTACAT TGGATTTGAG ACGGTGAATG GTGATATATT CATCTGTACC 
CAAAAAGCAG CCAGGAATAT GTCATACCAG GGCTTTACCA AAGACAATGG CGTGGTGCCT 
GTTGTTAAGG AATTAATGGG GGAGGAAATT CTTGGTGCAT CACTTTCTGC ACCTTTAACA 
TCATACAAGG TGATCTATGT TCTCCCAATG CTAACTATTA AGGAGGATAA AGGCACTGGT 
GTGGTTACAA GTGTTCCTTC CGACTCCCCT GATGATATTG CTGCCCTCAG AGACTTGAAG 
AAAAAGCAAG CCTTACGAGC AAAATATGGA ATTAGAGATG ACATGGTCTT GCCATTTGAG 
CCGGTGCCAG TCATTGAAAT CCCAGGTTTT GGAAATCTTT CTGCTGTAAC CATTTGTGAT 
GAGTTGAAAA TTCAGAGCCA GAATGACCGG GAAAAACTTG CAGAAGCAAA GGAGAAGATA 
TATCTAAAAG GATTTTATGA GGGTATCATG TTGGTGGATG GATTTAAAGG ACAGAAGGTT 
CAAGATGTAA AGAAGACTAT TCAGAAAAAG ATGATTGACG CTGGAGATGC ACTTATTTAC 
ATGGAACCAG AGAAACAAGT GATGTCCAGG TCGTCAGATG AATGTGTTGT GGCTCTGTGT 
GACCAGTGGT ACTTGGATTA TGGAGAAGAG AATTGGAAGA AACAGACATC TCAGTGCTTG 
AAGAACCTGG AAACATTCTG TGAGGAGACC AGGAGGAATT TTGAAGCCAC CTTAGGTTGG 
CTACAAGAAC ATGCTTGCTC AAGAACTTAT GGTCTAGGCA CTCACCTGCC TTGGGATGAG 
CAGTGGCTGA TTGAATCACT TTCTGACTCC ACTATTTACA TGGCATTTTA CACAGTTGCA 
CACCTATTGC AGGGGGGTAA CTTGCATGGA CAGGCAGAGT CTCCGCTGGG CATTAGACCG 
CAACAGATGA CCAAGGAAGT TTGGGATTAT GTTTTCTTCA AGGAGGCTCC ATTTCCTAAG 
ACTCAGATTG CAAAGGAAAA ATTAGATCAG TTAAAGCAGG AGTTTGAATT CTGGTATCCT 
GTTGATCTTC GCGTCTCTGG CAAGGATCTT GTTCCAAATC ATCTTTCATA TTACCTTTAT 
AATCATGTGG CTATGTGGCC GGAACAAAGT GACAAATGGC CTACAGCTGT GAGAGCAAAT 
GGACATCTCC TCCTGAACTC TGAGAAGATG TCAAAATCCA CAGGCAACTT CCTCACTTTG 
ACCCAAGCTA TTGACAAATT TTCAGCAGAT GGAATGCGTT TGGCTCTGGC TGATGCTGGT 
GACACTGTAG AAGATGCCAA CTTTGTGGAA GCCATGGCAG ATGCAGGTAT TCTCCGTCTG 
TACACCTGGG TAGAGTGGGT GAAAGAAATG GTTGCCAACT GGGACAGCCT AAGAAGTGGT 
CCTGCCAGCA CTTTCAATGA TAGAGTTTTT GCCAGTGAAT TGAATGCAGG AATTATAAAA 
ACAGATCAAA ACTATGAAAA GATGATGTTT AAAGAAGCTT TGAAAACAGG GTTTTTTGAG 
TTTCAGGCCG CAAAAGATAA GTACCGTGAA TTGGCTGTGG AAGGGATGCA CAGAGAACTT 
GTGTTCCGGT TTATTGAAGT TCAGACACTT CTCCTCGCTC CATTCTGTCC ACATTTGTGT 
GAGCACATCT GGACACTCCT GGGAAAGCCT GACTCAATTA TGAATGCTTC ATGGCCTGTG 
GCAGGTCCTG TTAATGAAGT TTTAATACAC TCCTCACAGT ATCTTATGGA AGTAACACAT 
GACCTTAGAC TACGACTCAA GAACTATATG ATGCCAGCTA AAGGGAAGAA GACTGACAAA 
CAACCCCTGC AGAAGCCCTC ACATTGCACC ATCTATGTGG CAAAGAACTA TCCACCTTGG 
CAACATACCA CCCTGTCTGT TCTACGTAAA CACTTTGAGG CCAATAACGG AAAACTGCCT 
GACAACAAAG TCATTGCTAG TGAACTAGGC AGTATGCCAG AACTGAAGAA ATACATGAAG 
AAAGTCATGC CATTTGTTGC CATGATTAAG GAAAATCTGG AGAAGATGGG GCCTCGTATT 
CTGGATTTGC AATTAGAATT TGATGAAAAG GCTGTGCTTA TGGAGAATAT AGTCTATCTG 
ACTAATTCGC TTGAGCTAGA ACACATAGAA GTCAAGTTTG CCTCCGAAGC AGAAGATAAA 
ATCAGGGAAG ACTGCTGTCC TGGGAAACCA CTTAATGTTT TTAGAATAGA ACCTGGTGTG 
TCCGTTTCTC TGGTGAATCC CCAGCCATCC AATGGCCACT TCTCAACCAA AATTGAAATC 
AGGCAAGGAG ATAACTGTGA TTCCATAATC AGGCGTTTAA TGAAAATGAA TCGAGGAATT 
AAAGACCTTT CCAAAGTGAA ACTGATGAGA TTTGATGATC CACTGTTGGG GCCTCGACGA 
GTTCCTGTCC TGGGAAAGGA GTACACCGAG AAGACCCCCA TTTCTGAGCA TGCTGTTTTC 
AATGTGGACC TCATGAGCAA GAAAATTCAT CTGACTGAGA ATGGGATAAG GGTGGATATT 
GGCGATACAA TAATCTATCT GGTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LARS1 ( NM_001317964.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304893.1 Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu67987
Clone ID Related Accession (Same CDS sequence)	NM_001317964.1
Accession Version	NM_001317964.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	leucine--tRNA ligase, cytoplasmic isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB091795.1, AB037773.1, AK304134.1, AC091887.3 and CK902545.1. On Dec 17, 2015 this sequence version replaced XM_011537655.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.120976.1, SRR1803613.226077.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG 
ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT 
TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA 
GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGAGTAAA 
GCTGCTGCTA AAGCTGGATC TTCTAAATAC CAGTGGGGCA TTATGAAATC CCTTGGCCTG 
TCTGATGAAG AGATAGTAAA ATTTTCTGAA GCAGAACATT GGCTTGATTA TTTCCCGCCA 
CTGGCTATTC AGGATTTAAA AAGAATGGGT TTGAAGGTAG ACTGGCGTCG TTCCTTCATC 
ACCACTGATG TTAATCCTTA CTATGATTCA TTTGTCAGAT GGCAATTTTT AACATTAAGA 
GAAAGAAACA AAATTAAATT TGGGAAGCGG TATACAATTT ACTCTCCGAA AGATGGACAG 
CCTTGCATGG ATCATGATAG ACAAACTGGA GAGGGTGTTG GACCTCAGGA ATATACTTTA 
CTCAAATTGA AGGTGCTTGA GCCATACCCA TCTAAATTAA GTGGCCTGAA AGGTAAAAAT 
ATTTTCTTGG TGGCTGCTAC TCTCAGACCT GAGACCATGT TTGGGCAGAC AAATTGTTGG 
GTTCGTCCTG ATATGAAGTA CATTGGATTT GAGACGGTGA ATGGTGATAT ATTCATCTGT 
ACCCAAAAAG CAGCCAGGAA TATGTCATAC CAGGGCTTTA CCAAAGACAA TGGCGTGGTG 
CCTGTTGTTA AGGAATTAAT GGGGGAGGAA ATTCTTGGTG CATCACTTTC TGCACCTTTA 
ACATCATACA AGGTGATCTA TGTTCTCCCA ATGCTAACTA TTAAGGAGGA TAAAGGCACT 
GGTGTGGTTA CAAGTGTTCC TTCCGACTCC CCTGATGATA TTGCTGCCCT CAGAGACTTG 
AAGAAAAAGC AAGCCTTACG AGCAAAATAT GGAATTAGAG ATGACATGGT CTTGCCATTT 
GAGCCGGTGC CAGTCATTGA AATCCCAGGT TTTGGAAATC TTTCTGCTGT AACCATTTGT 
GATGAGTTGA AAATTCAGAG CCAGAATGAC CGGGAAAAAC TTGCAGAAGC AAAGGAGAAG 
ATATATCTAA AAGGATTTTA TGAGGGTATC ATGTTGGTGG ATGGATTTAA AGGACAGAAG 
GTTCAAGATG TAAAGAAGAC TATTCAGAAA AAGATGATTG ACGCTGGAGA TGCACTTATT 
TACATGGAAC CAGAGAAACA AGTGATGTCC AGGTCGTCAG ATGAATGTGT TGTGGCTCTG 
TGTGACCAGT GGTACTTGGA TTATGGAGAA GAGAATTGGA AGAAACAGAC ATCTCAGTGC 
TTGAAGAACC TGGAAACATT CTGTGAGGAG ACCAGGAGGA ATTTTGAAGC CACCTTAGGT 
TGGCTACAAG AACATGCTTG CTCAAGAACT TATGGTCTAG GCACTCACCT GCCTTGGGAT 
GAGCAGTGGC TGATTGAATC ACTTTCTGAC TCCACTATTT ACATGGCATT TTACACAGTT 
GCACACCTAT TGCAGGGGGG TAACTTGCAT GGACAGGCAG AGTCTCCGCT GGGCATTAGA 
CCGCAACAGA TGACCAAGGA AGTTTGGGAT TATGTTTTCT TCAAGGAGGC TCCATTTCCT 
AAGACTCAGA TTGCAAAGGA AAAATTAGAT CAGTTAAAGC AGGAGTTTGA ATTCTGGTAT 
CCTGTTGATC TTCGCGTCTC TGGCAAGGAT CTTGTTCCAA ATCATCTTTC ATATTACCTT 
TATAATCATG TGGCTATGTG GCCGGAACAA AGTGACAAAT GGCCTACAGC TGTGAGAGCA 
AATGGACATC TCCTCCTGAA CTCTGAGAAG ATGTCAAAAT CCACAGGCAA CTTCCTCACT 
TTGACCCAAG CTATTGACAA ATTTTCAGCA GATGGAATGC GTTTGGCTCT GGCTGATGCT 
GGTGACACTG TAGAAGATGC CAACTTTGTG GAAGCCATGG CAGATGCAGG TATTCTCCGT 
CTGTACACCT GGGTAGAGTG GGTGAAAGAA ATGGTTGCCA ACTGGGACAG CCTAAGAAGT 
GGTCCTGCCA GCACTTTCAA TGATAGAGTT TTTGCCAGTG AATTGAATGC AGGAATTATA 
AAAACAGATC AAAACTATGA AAAGATGATG TTTAAAGAAG CTTTGAAAAC AGGGTTTTTT 
GAGTTTCAGG CCGCAAAAGA TAAGTACCGT GAATTGGCTG TGGAAGGGAT GCACAGAGAA 
CTTGTGTTCC GGTTTATTGA AGTTCAGACA CTTCTCCTCG CTCCATTCTG TCCACATTTG 
TGTGAGCACA TCTGGACACT CCTGGGAAAG CCTGACTCAA TTATGAATGC TTCATGGCCT 
GTGGCAGGTC CTGTTAATGA AGTTTTAATA CACTCCTCAC AGTATCTTAT GGAAGTAACA 
CATGACCTTA GACTACGACT CAAGAACTAT ATGATGCCAG CTAAAGGGAA GAAGACTGAC 
AAACAACCCC TGCAGAAGCC CTCACATTGC ACCATCTATG TGGCAAAGAA CTATCCACCT 
TGGCAACATA CCACCCTGTC TGTTCTACGT AAACACTTTG AGGCCAATAA CGGAAAACTG 
CCTGACAACA AAGTCATTGC TAGTGAACTA GGCAGTATGC CAGAACTGAA GAAATACATG 
AAGAAAGTCA TGCCATTTGT TGCCATGATT AAGGAAAATC TGGAGAAGAT GGGGCCTCGT 
ATTCTGGATT TGCAATTAGA ATTTGATGAA AAGGCTGTGC TTATGGAGAA TATAGTCTAT 
CTGACTAATT CGCTTGAGCT AGAACACATA GAAGTCAAGT TTGCCTCCGA AGCAGAAGAT 
AAAATCAGGG AAGACTGCTG TCCTGGGAAA CCACTTAATG TTTTTAGAAT AGAACCTGGT 
GTGTCCGTTT CTCTGGTGAA TCCCCAGCCA TCCAATGGCC ACTTCTCAAC CAAAATTGAA 
ATCAGGCAAG GAGATAACTG TGATTCCATA ATCAGGCGTT TAATGAAAAT GAATCGAGGA 
ATTAAAGACC TTTCCAAAGT GAAACTGATG AGATTTGATG ATCCACTGTT GGGGCCTCGA 
CGAGTTCCTG TCCTGGGAAA GGAGTACACC GAGAAGACCC CCATTTCTGA GCATGCTGTT 
TTCAATGTGG ACCTCATGAG CAAGAAAATT CATCTGACTG AGAATGGGAT AAGGGTGGAT 
ATTGGCGATA CAATAATCTA TCTGGTTCAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304893.1
CDS	295..3687
Translation	MAERKGTAKVDFLKKIEKEIQQKWDTERVFEVNASNLEKQTSKGKYFVTFPYPYMNGRLHLGHTFSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIKSKAAAKAGSSKYQWGIMKSLGLSDEEIVKFSEAEHWLDYFPPLAIQDLKRMGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILGASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIRDDMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDVKKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFCEETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSKSTGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVANWDSLRSGPASTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYRELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVNEVLIHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKLPDNKVIASELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAEDKIREDCCPGKPLNVFRIEPGVSVSLVNPQPSNGHFSTKIEIRQGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDDPLLGPRRVPVLGKEYTEKTPISEHAVFNVDLMSKKIHLTENGIRVDIGDTIIYLVH

Target ORF information:

RefSeq Version	NM_001317964.1
Organism	Homo sapiens(human)
Definition	Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317964.1

ORF Insert Sequence:

ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG 
ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT 
TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA 
GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGAGTAAA 
GCTGCTGCTA AAGCTGGATC TTCTAAATAC CAGTGGGGCA TTATGAAATC CCTTGGCCTG 
TCTGATGAAG AGATAGTAAA ATTTTCTGAA GCAGAACATT GGCTTGATTA TTTCCCGCCA 
CTGGCTATTC AGGATTTAAA AAGAATGGGT TTGAAGGTAG ACTGGCGTCG TTCCTTCATC 
ACCACTGATG TTAATCCTTA CTATGATTCA TTTGTCAGAT GGCAATTTTT AACATTAAGA 
GAAAGAAACA AAATTAAATT TGGGAAGCGG TATACAATTT ACTCTCCGAA AGATGGACAG 
CCTTGCATGG ATCATGATAG ACAAACTGGA GAGGGTGTTG GACCTCAGGA ATATACTTTA 
CTCAAATTGA AGGTGCTTGA GCCATACCCA TCTAAATTAA GTGGCCTGAA AGGTAAAAAT 
ATTTTCTTGG TGGCTGCTAC TCTCAGACCT GAGACCATGT TTGGGCAGAC AAATTGTTGG 
GTTCGTCCTG ATATGAAGTA CATTGGATTT GAGACGGTGA ATGGTGATAT ATTCATCTGT 
ACCCAAAAAG CAGCCAGGAA TATGTCATAC CAGGGCTTTA CCAAAGACAA TGGCGTGGTG 
CCTGTTGTTA AGGAATTAAT GGGGGAGGAA ATTCTTGGTG CATCACTTTC TGCACCTTTA 
ACATCATACA AGGTGATCTA TGTTCTCCCA ATGCTAACTA TTAAGGAGGA TAAAGGCACT 
GGTGTGGTTA CAAGTGTTCC TTCCGACTCC CCTGATGATA TTGCTGCCCT CAGAGACTTG 
AAGAAAAAGC AAGCCTTACG AGCAAAATAT GGAATTAGAG ATGACATGGT CTTGCCATTT 
GAGCCGGTGC CAGTCATTGA AATCCCAGGT TTTGGAAATC TTTCTGCTGT AACCATTTGT 
GATGAGTTGA AAATTCAGAG CCAGAATGAC CGGGAAAAAC TTGCAGAAGC AAAGGAGAAG 
ATATATCTAA AAGGATTTTA TGAGGGTATC ATGTTGGTGG ATGGATTTAA AGGACAGAAG 
GTTCAAGATG TAAAGAAGAC TATTCAGAAA AAGATGATTG ACGCTGGAGA TGCACTTATT 
TACATGGAAC CAGAGAAACA AGTGATGTCC AGGTCGTCAG ATGAATGTGT TGTGGCTCTG 
TGTGACCAGT GGTACTTGGA TTATGGAGAA GAGAATTGGA AGAAACAGAC ATCTCAGTGC 
TTGAAGAACC TGGAAACATT CTGTGAGGAG ACCAGGAGGA ATTTTGAAGC CACCTTAGGT 
TGGCTACAAG AACATGCTTG CTCAAGAACT TATGGTCTAG GCACTCACCT GCCTTGGGAT 
GAGCAGTGGC TGATTGAATC ACTTTCTGAC TCCACTATTT ACATGGCATT TTACACAGTT 
GCACACCTAT TGCAGGGGGG TAACTTGCAT GGACAGGCAG AGTCTCCGCT GGGCATTAGA 
CCGCAACAGA TGACCAAGGA AGTTTGGGAT TATGTTTTCT TCAAGGAGGC TCCATTTCCT 
AAGACTCAGA TTGCAAAGGA AAAATTAGAT CAGTTAAAGC AGGAGTTTGA ATTCTGGTAT 
CCTGTTGATC TTCGCGTCTC TGGCAAGGAT CTTGTTCCAA ATCATCTTTC ATATTACCTT 
TATAATCATG TGGCTATGTG GCCGGAACAA AGTGACAAAT GGCCTACAGC TGTGAGAGCA 
AATGGACATC TCCTCCTGAA CTCTGAGAAG ATGTCAAAAT CCACAGGCAA CTTCCTCACT 
TTGACCCAAG CTATTGACAA ATTTTCAGCA GATGGAATGC GTTTGGCTCT GGCTGATGCT 
GGTGACACTG TAGAAGATGC CAACTTTGTG GAAGCCATGG CAGATGCAGG TATTCTCCGT 
CTGTACACCT GGGTAGAGTG GGTGAAAGAA ATGGTTGCCA ACTGGGACAG CCTAAGAAGT 
GGTCCTGCCA GCACTTTCAA TGATAGAGTT TTTGCCAGTG AATTGAATGC AGGAATTATA 
AAAACAGATC AAAACTATGA AAAGATGATG TTTAAAGAAG CTTTGAAAAC AGGGTTTTTT 
GAGTTTCAGG CCGCAAAAGA TAAGTACCGT GAATTGGCTG TGGAAGGGAT GCACAGAGAA 
CTTGTGTTCC GGTTTATTGA AGTTCAGACA CTTCTCCTCG CTCCATTCTG TCCACATTTG 
TGTGAGCACA TCTGGACACT CCTGGGAAAG CCTGACTCAA TTATGAATGC TTCATGGCCT 
GTGGCAGGTC CTGTTAATGA AGTTTTAATA CACTCCTCAC AGTATCTTAT GGAAGTAACA 
CATGACCTTA GACTACGACT CAAGAACTAT ATGATGCCAG CTAAAGGGAA GAAGACTGAC 
AAACAACCCC TGCAGAAGCC CTCACATTGC ACCATCTATG TGGCAAAGAA CTATCCACCT 
TGGCAACATA CCACCCTGTC TGTTCTACGT AAACACTTTG AGGCCAATAA CGGAAAACTG 
CCTGACAACA AAGTCATTGC TAGTGAACTA GGCAGTATGC CAGAACTGAA GAAATACATG 
AAGAAAGTCA TGCCATTTGT TGCCATGATT AAGGAAAATC TGGAGAAGAT GGGGCCTCGT 
ATTCTGGATT TGCAATTAGA ATTTGATGAA AAGGCTGTGC TTATGGAGAA TATAGTCTAT 
CTGACTAATT CGCTTGAGCT AGAACACATA GAAGTCAAGT TTGCCTCCGA AGCAGAAGAT 
AAAATCAGGG AAGACTGCTG TCCTGGGAAA CCACTTAATG TTTTTAGAAT AGAACCTGGT 
GTGTCCGTTT CTCTGGTGAA TCCCCAGCCA TCCAATGGCC ACTTCTCAAC CAAAATTGAA 
ATCAGGCAAG GAGATAACTG TGATTCCATA ATCAGGCGTT TAATGAAAAT GAATCGAGGA 
ATTAAAGACC TTTCCAAAGT GAAACTGATG AGATTTGATG ATCCACTGTT GGGGCCTCGA 
CGAGTTCCTG TCCTGGGAAA GGAGTACACC GAGAAGACCC CCATTTCTGA GCATGCTGTT 
TTCAATGTGG ACCTCATGAG CAAGAAAATT CATCTGACTG AGAATGGGAT AAGGGTGGAT 
ATTGGCGATA CAATAATCTA TCTGGTTCAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Leucine-induced localization of Leucyl-tRNA synthetase in lysosome membrane.
Biochemical and biophysical research communications493(2)1129-1135(2017 11)
Choi H,Son JB,Kang J,Kwon J,Kim JH,Jung M,Kim SK,Kim S,Mun JY

Control of leucine-dependent mTORC1 pathway through chemical intervention of leucyl-tRNA synthetase and RagD interaction.
Nature communications8(1)732(2017 09)
Kim JH,Lee C,Lee M,Wang H,Kim K,Park SJ,Yoon I,Jang J,Zhao H,Kim HK,Kwon NH,Jeong SJ,Yoo HC,Kim JH,Yang JS,Lee MY,Lee CW,Yun J,Oh SJ,Kang JS,Martinis SA,Hwang KY,Guo M,Han G,Han JM,Kim S

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

The crystal structure of human GlnRS provides basis for the development of neurological disorders.
Nucleic acids research44(7)3420-31(2016 Apr)
Ognjenovi? J,Wu J,Matthies D,Baxa U,Subramaniam S,Ling J,Simonovi? M

Modulation of Aminoacylation and Editing Properties of Leucyl-tRNA Synthetase by a Conserved Structural Module.
The Journal of biological chemistry290(19)12256-67(2015 May)
Yan W,Ye Q,Tan M,Chen X,Eriani G,Wang ED

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LARS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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