lars-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	lars-1
Entrez Gene ID	175538
Full Name	Leucine--tRNA ligase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_065436.6	NP_497837.1	Leucine--tRNA ligase
NM_065436.5	NP_497837.1	Leucine--tRNA ligase

Pathways from BioSystems

Homologies

Homo sapiens (human)	LARS	NP_064502.9
Drosophila melanogaster (fruit fly)	CG33123	NP_787968.1
Neurospora crassa	NCU09463	XP_963218.1
Oryza sativa (rice)	Os09g0378300	NP_001063048.1
Rattus norvegicus (Norway rat)	Lars	NP_001009637.1
Danio rerio (zebrafish)	larsb	XP_698279.5
Caenorhabditis elegans (roundworm)	lars-1	NP_497837.1
Saccharomyces cerevisiae (baker's yeast)	CDC60	NP_015165.1
Arabidopsis thaliana (thale cress)	AT1G09620	NP_172433.2
Oryza sativa (rice)	Os09g0503400	NP_001063593.2
Xenopus tropicalis (tropical clawed frog)	lars	NP_001015831.1
Canis lupus familiaris (dog)	LARS	XP_535229.3
Mus musculus (house mouse)	Lars	NP_598898.2
Gallus gallus (chicken)	LARS	XP_414663.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008297	XP_317169.2
Kluyveromyces lactis	KLLA0D06105g	XP_453334.1
Magnaporthe oryzae (rice blast fungus)	MGG_02432	XP_003709219.1
Pan troglodytes (chimpanzee)	LARS	XP_001157096.1
Bos taurus (cattle)	LARS	NP_001095962.1
Eremothecium gossypii	AGOS_AAL088W	NP_982454.2
Schizosaccharomyces pombe (fission yeast)	lrs1	NP_594882.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following lars-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lars-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa341933	NM_065436.6 Latest version!	Caenorhabditis elegans Leucine--tRNA ligase (lars-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa341933	NM_065436.5	Caenorhabditis elegans Leucine--tRNA ligase (lars-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lars-1 ( NM_065436.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_497837.1 Caenorhabditis elegans Leucine--tRNA ligase (lars-1), mRNA.

Price & Availability↑

CloneID	OCa341933
Clone ID Related Accession (Same CDS sequence)	NM_065436.6 , NM_065436.5
Accession Version	NM_065436.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Leucine--tRNA ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_065436.5.

ATGTCGAAAA TCAATAAGGA GCGGAAGAAG GTTGCTCAAC TTCAAGAAAT TGAAAAGAGC 
ATTCAGGAGC TGTGGGAGAG CAAGAAGGCT TTCGAGGCGG ATGCTAGGGA TGATGGAAAA 
CCAAAGTACC TCGTCACGTT TCCCTTCCCA TACATGAATG GACGTCTTCA TTTGGGACAC 
ACATTCTCAG CTTCAAAATG TGAATTTGCC GCCGGTTTCC AACGTCTTCA AGGCAAAGAA 
GTGCTCTTTC CATTCGGATT TCATTGTACT GGAATGCCAA TTAAAGCATG TGCAGATAAA 
TTGAAAAGAG AAATGCAAGA TTTCGGATAT CCACCAAATT TCCCGGAAGA TGTTGTCGAG 
GAAGTTAAAG AAGAAGTTTC GGCTGTCGAT GAGATTATCA AGGATAAGAG TAAAGGAAAG 
AAGAGTAAAT TGGTGGCAAA AACTGGAAAT GCAAAATACC AATGGCAAAT TATGAAGTCT 
CTGGGATTGT GTGATGAGGA AATTAAGGAA TTCTCGGATC CAAATCATTG GCTATATTAT 
TTCCCACCAC ATTGCATTGC TGATCTTAAG AAAATGGGAC TAAAGGCTGA TTGGCGTCGT 
TCCTTCATCA CCACCGACGT GAATCCATAC TTCGACTCAT TCGTCCGTTG GCAGTTCAAC 
CTTCTTCGTG CAGCTAAGAA GATCGATTTC GGAAAGAGAT ATACAATTTA TTCACCGAAA 
GACGGTCAAC CATGCATGGA TCACGATCGT GCCAGTGGAG AAGGTGTTGG TCCACAAGAA 
TATACTCTTA TTAAACTGAA AGTTCTTGAT CCAAAACCTC AAGCTTTGAG CCATATCAAA 
GAGGATATTT ATCTCGTTGC GGCTACTTTG AGACCTGAAA CAATGTACGG ACAAACGAAT 
TGTTATCTCC ATCCAGATAT TCAATATTCC GTGTTTTATG CGACGGAAAA CGAGAAACAA 
GTATTTGTGG CAACTGCCAG ATCTGCTAGA ATCATGTCAT ATCAAGGATT GACTAAGGAA 
AATGGTAAAG TCAACTATGT CCTGGGACTC GAGAAAATCG CTGGAAGCAA GATTCTCGGA 
GCTCCACTCT CTGCGCCTCT CGCAAAATAC GAAAGAGTTT ATGCATTGCC AATGTTGACT 
ATCAAGGATG ATAAGGGAAC TGGTGTTGTT ACAAGTGTAC CATCAGACTC TCCAGATGAT 
TTTGCAGCTT TGTCGGATCT CAAGAAGAAG AAGCCATTGA GAGAAAAATA TGGATTGACT 
GATGAGATGG TTCTTCCATT CGAACCAGTT CCAATTATTA AAATCGAAGG ACTTGGGGAT 
TTGGCTGCAG TTGAAATGTG CTCAAGGCTG AAAATTGAGT CTCAAAATGA GAAGGACAAA 
CTGGAAGAGG CGAAGAAAGA AGTTTATTTG AAAGGATTCT ACGATGGAGT TATGCTCGTC 
GGAAAGTATG CTGGAAAGAA GACAGCCGAC GTCAAGAAAG TTATTCAAGA TGATTTGACA 
GCTGAAGGTC TTGCCACGAA ATATGTGGAG CCTGAGAAGA AGGTGATGAG CAGATCTGGA 
GATGAGTGCG TCGTTGCGTT GTGTGATCAA TGGTATTTGA ATTATGGAGA GGCTGAATGG 
AAGGCTGCTG CAAAGAAGGT TCTTGAGCCA CTCAGAACTT TCAATGATGA GACCCGCCGT 
GGATTGGAAA CCACAGTCGA TTGGCTCCAC GAGTATGCTT GCTCCAGAAG TTACGGTCTT 
GGAACCAAAC TTCCATGGGA TACTCAATAC CTCATCGAAT CTCTGTCAGA CTCTACAATC 
TACAATGCTT ATTATACAGT TGCTCATCTT CTCCAACAGG GAGCTTTTGA TGGATCAGTC 
GTCGGACCTG CTGGAATTAA AGCTGATCAG ATGACAGATG CTTCGTGGAG TTATGTATTC 
CTCGGAGAAA TTTACGATTC GAAAACCATG CCGGTTGAGG AGGAAAAGCT TAAATCATTG 
AGGAAAGAAT TCATGTATTG GTATCCAATT GATATGAGAG CATCTGGAAA AGATCTTATC 
GGAAATCATT TGACATATCT TCTGTTCAAT CATGCTGCAA TCTGGCCAAC AGACACTTCA 
AAATGGCCAA AAGGAATTCG GGCCAATGGA CATTTGCTCT TGAACAATGA GAAAATGTCG 
AAATCCACTG GAAACTTTAT GACATTGGAA GAAGCCATCG AAAAGTTCAG CGCAGATGGT 
ATGAGATTGA GTCTTGCCGA TGCTGGGGAT GGTTTGGAAG ATGCTAACTT TGTGTACGCA 
ATGGCAGATG CAGCTATTCT GAGACTATTC ACTATGATCG AATGGATCAA GGAAATGATT 
GAACAACGGG ATGCCGGACT TCTCCGAAAA GATGCAAAGA AATTCGCTGA TCGAGTTTTT 
GCAAATGAAA TGAATTCACT GATCGCTGCC ACAGAACAAA ATTATGAAGC TACCAACTTC 
AAGGATGCAC TGAAAACTGG ATTCTTCGAA TATCAAGCCA TTAGAGATAC ATACAGAGAA 
CTTTGCGCTG GAATTGATGA GCCAATGAGC GAATCGCTTG TCTTTAGATT CATTGAAACT 
CAGATGCTCA TCCTATCACC AATCTGCCCA CATATTGCTG AATACATCTG GCAATTGCTC 
GGAAAAGACG GCTTGATTGT AAATGCTCCA TGGCCAACTG TCGATCCTGT CGATGAGAAG 
CTCGCCATTG GTGCTCGTTT CATCACGGAA TCGCTGGCCG AATTCCGTGC CCGACTGAAA 
ACCTACATGA CTCCAAAGAA GAAGGCACTG AAGGAGATTC CAGAAGTTCC AACTGAAGCA 
GTGATCTATG TTGCTAAAGA ATATCCACCA TGGCAGAAGA CTATTCTTGA TATTCTTGAG 
AAACAAGCCA AGGCTAATAA TGGAGCACTT CCAGATAATA AAGCAATCAG TCAGCTCATT 
GGAAAAGAAG AATCACTCAA GAAATTCGCC AAAAAAGCGA TGCCATTCGT TCAAATGATC 
AAGGAAAGAT TCGAACAGAA GGGTGTTTCA GCATTGGCTT CTTCTAGTCC AGTTGATCAA 
ACTTCAATTC TCAACGAGAA TATTGATTTT ATTATGAACG CTTTGGATTT GGATCGTGTT 
ACTATTCGAC ATACTGATGA GGAGGGAATT GATGCGAATA TTGTTGAGAC TACAGTACCG 
CTGGTGCCAA TGTTGAATTT CACGCCGAAT AGACCAACAA TCAAACTTGT CGCCAGAAAT 
GTTCAAATCT GCAATGCAAT GTTCGATGTT GATGTACCAA TTGTTAATGG AGACAGTGTT 
TCAATGGTTA TTCGGAAGAT GAGACGCATC AGCAAGGCAA TCAAACCGAA ATTCGAAGTG 
AGCCTCTGGA GATACAAGAA CGCGGTTTGG GGAGATCGAC AGATGATTTC GTACAGAAAT 
CCATTCGAGG AGAACATTCA ATTGTCCGAC GCAGACATTT TCAATTTCGA GGCTGATAAC 
AAGATTTCTG TGACTTCTGG AAGTGAAAAG TTCGATCTTG GGCGTACTAT TGTGTACAAG 
GCTAACGTCC CAGAAAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_497837.1
CDS	42..3602
Translation	MSKINKERKKVAQLQEIEKSIQELWESKKAFEADARDDGKPKYLVTFPFPYMNGRLHLGHTFSASKCEFAAGFQRLQGKEVLFPFGFHCTGMPIKACADKLKREMQDFGYPPNFPEDVVEEVKEEVSAVDEIIKDKSKGKKSKLVAKTGNAKYQWQIMKSLGLCDEEIKEFSDPNHWLYYFPPHCIADLKKMGLKADWRRSFITTDVNPYFDSFVRWQFNLLRAAKKIDFGKRYTIYSPKDGQPCMDHDRASGEGVGPQEYTLIKLKVLDPKPQALSHIKEDIYLVAATLRPETMYGQTNCYLHPDIQYSVFYATENEKQVFVATARSARIMSYQGLTKENGKVNYVLGLEKIAGSKILGAPLSAPLAKYERVYALPMLTIKDDKGTGVVTSVPSDSPDDFAALSDLKKKKPLREKYGLTDEMVLPFEPVPIIKIEGLGDLAAVEMCSRLKIESQNEKDKLEEAKKEVYLKGFYDGVMLVGKYAGKKTADVKKVIQDDLTAEGLATKYVEPEKKVMSRSGDECVVALCDQWYLNYGEAEWKAAAKKVLEPLRTFNDETRRGLETTVDWLHEYACSRSYGLGTKLPWDTQYLIESLSDSTIYNAYYTVAHLLQQGAFDGSVVGPAGIKADQMTDASWSYVFLGEIYDSKTMPVEEEKLKSLRKEFMYWYPIDMRASGKDLIGNHLTYLLFNHAAIWPTDTSKWPKGIRANGHLLLNNEKMSKSTGNFMTLEEAIEKFSADGMRLSLADAGDGLEDANFVYAMADAAILRLFTMIEWIKEMIEQRDAGLLRKDAKKFADRVFANEMNSLIAATEQNYEATNFKDALKTGFFEYQAIRDTYRELCAGIDEPMSESLVFRFIETQMLILSPICPHIAEYIWQLLGKDGLIVNAPWPTVDPVDEKLAIGARFITESLAEFRARLKTYMTPKKKALKEIPEVPTEAVIYVAKEYPPWQKTILDILEKQAKANNGALPDNKAISQLIGKEESLKKFAKKAMPFVQMIKERFEQKGVSALASSSPVDQTSILNENIDFIMNALDLDRVTIRHTDEEGIDANIVETTVPLVPMLNFTPNRPTIKLVARNVQICNAMFDVDVPIVNGDSVSMVIRKMRRISKAIKPKFEVSLWRYKNAVWGDRQMISYRNPFEENIQLSDADIFNFEADNKISVTSGSEKFDLGRTIVYKANVPEN

Target ORF information:

RefSeq Version	NM_065436.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Leucine--tRNA ligase (lars-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065436.6

ORF Insert Sequence:

ATGTCGAAAA TCAATAAGGA GCGGAAGAAG GTTGCTCAAC TTCAAGAAAT TGAAAAGAGC 
ATTCAGGAGC TGTGGGAGAG CAAGAAGGCT TTCGAGGCGG ATGCTAGGGA TGATGGAAAA 
CCAAAGTACC TCGTCACGTT TCCCTTCCCA TACATGAATG GACGTCTTCA TTTGGGACAC 
ACATTCTCAG CTTCAAAATG TGAATTTGCC GCCGGTTTCC AACGTCTTCA AGGCAAAGAA 
GTGCTCTTTC CATTCGGATT TCATTGTACT GGAATGCCAA TTAAAGCATG TGCAGATAAA 
TTGAAAAGAG AAATGCAAGA TTTCGGATAT CCACCAAATT TCCCGGAAGA TGTTGTCGAG 
GAAGTTAAAG AAGAAGTTTC GGCTGTCGAT GAGATTATCA AGGATAAGAG TAAAGGAAAG 
AAGAGTAAAT TGGTGGCAAA AACTGGAAAT GCAAAATACC AATGGCAAAT TATGAAGTCT 
CTGGGATTGT GTGATGAGGA AATTAAGGAA TTCTCGGATC CAAATCATTG GCTATATTAT 
TTCCCACCAC ATTGCATTGC TGATCTTAAG AAAATGGGAC TAAAGGCTGA TTGGCGTCGT 
TCCTTCATCA CCACCGACGT GAATCCATAC TTCGACTCAT TCGTCCGTTG GCAGTTCAAC 
CTTCTTCGTG CAGCTAAGAA GATCGATTTC GGAAAGAGAT ATACAATTTA TTCACCGAAA 
GACGGTCAAC CATGCATGGA TCACGATCGT GCCAGTGGAG AAGGTGTTGG TCCACAAGAA 
TATACTCTTA TTAAACTGAA AGTTCTTGAT CCAAAACCTC AAGCTTTGAG CCATATCAAA 
GAGGATATTT ATCTCGTTGC GGCTACTTTG AGACCTGAAA CAATGTACGG ACAAACGAAT 
TGTTATCTCC ATCCAGATAT TCAATATTCC GTGTTTTATG CGACGGAAAA CGAGAAACAA 
GTATTTGTGG CAACTGCCAG ATCTGCTAGA ATCATGTCAT ATCAAGGATT GACTAAGGAA 
AATGGTAAAG TCAACTATGT CCTGGGACTC GAGAAAATCG CTGGAAGCAA GATTCTCGGA 
GCTCCACTCT CTGCGCCTCT CGCAAAATAC GAAAGAGTTT ATGCATTGCC AATGTTGACT 
ATCAAGGATG ATAAGGGAAC TGGTGTTGTT ACAAGTGTAC CATCAGACTC TCCAGATGAT 
TTTGCAGCTT TGTCGGATCT CAAGAAGAAG AAGCCATTGA GAGAAAAATA TGGATTGACT 
GATGAGATGG TTCTTCCATT CGAACCAGTT CCAATTATTA AAATCGAAGG ACTTGGGGAT 
TTGGCTGCAG TTGAAATGTG CTCAAGGCTG AAAATTGAGT CTCAAAATGA GAAGGACAAA 
CTGGAAGAGG CGAAGAAAGA AGTTTATTTG AAAGGATTCT ACGATGGAGT TATGCTCGTC 
GGAAAGTATG CTGGAAAGAA GACAGCCGAC GTCAAGAAAG TTATTCAAGA TGATTTGACA 
GCTGAAGGTC TTGCCACGAA ATATGTGGAG CCTGAGAAGA AGGTGATGAG CAGATCTGGA 
GATGAGTGCG TCGTTGCGTT GTGTGATCAA TGGTATTTGA ATTATGGAGA GGCTGAATGG 
AAGGCTGCTG CAAAGAAGGT TCTTGAGCCA CTCAGAACTT TCAATGATGA GACCCGCCGT 
GGATTGGAAA CCACAGTCGA TTGGCTCCAC GAGTATGCTT GCTCCAGAAG TTACGGTCTT 
GGAACCAAAC TTCCATGGGA TACTCAATAC CTCATCGAAT CTCTGTCAGA CTCTACAATC 
TACAATGCTT ATTATACAGT TGCTCATCTT CTCCAACAGG GAGCTTTTGA TGGATCAGTC 
GTCGGACCTG CTGGAATTAA AGCTGATCAG ATGACAGATG CTTCGTGGAG TTATGTATTC 
CTCGGAGAAA TTTACGATTC GAAAACCATG CCGGTTGAGG AGGAAAAGCT TAAATCATTG 
AGGAAAGAAT TCATGTATTG GTATCCAATT GATATGAGAG CATCTGGAAA AGATCTTATC 
GGAAATCATT TGACATATCT TCTGTTCAAT CATGCTGCAA TCTGGCCAAC AGACACTTCA 
AAATGGCCAA AAGGAATTCG GGCCAATGGA CATTTGCTCT TGAACAATGA GAAAATGTCG 
AAATCCACTG GAAACTTTAT GACATTGGAA GAAGCCATCG AAAAGTTCAG CGCAGATGGT 
ATGAGATTGA GTCTTGCCGA TGCTGGGGAT GGTTTGGAAG ATGCTAACTT TGTGTACGCA 
ATGGCAGATG CAGCTATTCT GAGACTATTC ACTATGATCG AATGGATCAA GGAAATGATT 
GAACAACGGG ATGCCGGACT TCTCCGAAAA GATGCAAAGA AATTCGCTGA TCGAGTTTTT 
GCAAATGAAA TGAATTCACT GATCGCTGCC ACAGAACAAA ATTATGAAGC TACCAACTTC 
AAGGATGCAC TGAAAACTGG ATTCTTCGAA TATCAAGCCA TTAGAGATAC ATACAGAGAA 
CTTTGCGCTG GAATTGATGA GCCAATGAGC GAATCGCTTG TCTTTAGATT CATTGAAACT 
CAGATGCTCA TCCTATCACC AATCTGCCCA CATATTGCTG AATACATCTG GCAATTGCTC 
GGAAAAGACG GCTTGATTGT AAATGCTCCA TGGCCAACTG TCGATCCTGT CGATGAGAAG 
CTCGCCATTG GTGCTCGTTT CATCACGGAA TCGCTGGCCG AATTCCGTGC CCGACTGAAA 
ACCTACATGA CTCCAAAGAA GAAGGCACTG AAGGAGATTC CAGAAGTTCC AACTGAAGCA 
GTGATCTATG TTGCTAAAGA ATATCCACCA TGGCAGAAGA CTATTCTTGA TATTCTTGAG 
AAACAAGCCA AGGCTAATAA TGGAGCACTT CCAGATAATA AAGCAATCAG TCAGCTCATT 
GGAAAAGAAG AATCACTCAA GAAATTCGCC AAAAAAGCGA TGCCATTCGT TCAAATGATC 
AAGGAAAGAT TCGAACAGAA GGGTGTTTCA GCATTGGCTT CTTCTAGTCC AGTTGATCAA 
ACTTCAATTC TCAACGAGAA TATTGATTTT ATTATGAACG CTTTGGATTT GGATCGTGTT 
ACTATTCGAC ATACTGATGA GGAGGGAATT GATGCGAATA TTGTTGAGAC TACAGTACCG 
CTGGTGCCAA TGTTGAATTT CACGCCGAAT AGACCAACAA TCAAACTTGT CGCCAGAAAT 
GTTCAAATCT GCAATGCAAT GTTCGATGTT GATGTACCAA TTGTTAATGG AGACAGTGTT 
TCAATGGTTA TTCGGAAGAT GAGACGCATC AGCAAGGCAA TCAAACCGAA ATTCGAAGTG 
AGCCTCTGGA GATACAAGAA CGCGGTTTGG GGAGATCGAC AGATGATTTC GTACAGAAAT 
CCATTCGAGG AGAACATTCA ATTGTCCGAC GCAGACATTT TCAATTTCGA GGCTGATAAC 
AAGATTTCTG TGACTTCTGG AAGTGAAAAG TTCGATCTTG GGCGTACTAT TGTGTACAAG 
GCTAACGTCC CAGAAAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lars-1 ( NM_065436.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_497837.1 Caenorhabditis elegans Leucine--tRNA ligase (lars-1), partial mRNA.

Price & Availability↑

CloneID	OCa341933
Clone ID Related Accession (Same CDS sequence)	NM_065436.6 , NM_065436.5
Accession Version	NM_065436.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Leucine--tRNA ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_065436.4. COMPLETENESS: incomplete on both ends.

ATGTCGAAAA TCAATAAGGA GCGGAAGAAG GTTGCTCAAC TTCAAGAAAT TGAAAAGAGC 
ATTCAGGAGC TGTGGGAGAG CAAGAAGGCT TTCGAGGCGG ATGCTAGGGA TGATGGAAAA 
CCAAAGTACC TCGTCACGTT TCCCTTCCCA TACATGAATG GACGTCTTCA TTTGGGACAC 
ACATTCTCAG CTTCAAAATG TGAATTTGCC GCCGGTTTCC AACGTCTTCA AGGCAAAGAA 
GTGCTCTTTC CATTCGGATT TCATTGTACT GGAATGCCAA TTAAAGCATG TGCAGATAAA 
TTGAAAAGAG AAATGCAAGA TTTCGGATAT CCACCAAATT TCCCGGAAGA TGTTGTCGAG 
GAAGTTAAAG AAGAAGTTTC GGCTGTCGAT GAGATTATCA AGGATAAGAG TAAAGGAAAG 
AAGAGTAAAT TGGTGGCAAA AACTGGAAAT GCAAAATACC AATGGCAAAT TATGAAGTCT 
CTGGGATTGT GTGATGAGGA AATTAAGGAA TTCTCGGATC CAAATCATTG GCTATATTAT 
TTCCCACCAC ATTGCATTGC TGATCTTAAG AAAATGGGAC TAAAGGCTGA TTGGCGTCGT 
TCCTTCATCA CCACCGACGT GAATCCATAC TTCGACTCAT TCGTCCGTTG GCAGTTCAAC 
CTTCTTCGTG CAGCTAAGAA GATCGATTTC GGAAAGAGAT ATACAATTTA TTCACCGAAA 
GACGGTCAAC CATGCATGGA TCACGATCGT GCCAGTGGAG AAGGTGTTGG TCCACAAGAA 
TATACTCTTA TTAAACTGAA AGTTCTTGAT CCAAAACCTC AAGCTTTGAG CCATATCAAA 
GAGGATATTT ATCTCGTTGC GGCTACTTTG AGACCTGAAA CAATGTACGG ACAAACGAAT 
TGTTATCTCC ATCCAGATAT TCAATATTCC GTGTTTTATG CGACGGAAAA CGAGAAACAA 
GTATTTGTGG CAACTGCCAG ATCTGCTAGA ATCATGTCAT ATCAAGGATT GACTAAGGAA 
AATGGTAAAG TCAACTATGT CCTGGGACTC GAGAAAATCG CTGGAAGCAA GATTCTCGGA 
GCTCCACTCT CTGCGCCTCT CGCAAAATAC GAAAGAGTTT ATGCATTGCC AATGTTGACT 
ATCAAGGATG ATAAGGGAAC TGGTGTTGTT ACAAGTGTAC CATCAGACTC TCCAGATGAT 
TTTGCAGCTT TGTCGGATCT CAAGAAGAAG AAGCCATTGA GAGAAAAATA TGGATTGACT 
GATGAGATGG TTCTTCCATT CGAACCAGTT CCAATTATTA AAATCGAAGG ACTTGGGGAT 
TTGGCTGCAG TTGAAATGTG CTCAAGGCTG AAAATTGAGT CTCAAAATGA GAAGGACAAA 
CTGGAAGAGG CGAAGAAAGA AGTTTATTTG AAAGGATTCT ACGATGGAGT TATGCTCGTC 
GGAAAGTATG CTGGAAAGAA GACAGCCGAC GTCAAGAAAG TTATTCAAGA TGATTTGACA 
GCTGAAGGTC TTGCCACGAA ATATGTGGAG CCTGAGAAGA AGGTGATGAG CAGATCTGGA 
GATGAGTGCG TCGTTGCGTT GTGTGATCAA TGGTATTTGA ATTATGGAGA GGCTGAATGG 
AAGGCTGCTG CAAAGAAGGT TCTTGAGCCA CTCAGAACTT TCAATGATGA GACCCGCCGT 
GGATTGGAAA CCACAGTCGA TTGGCTCCAC GAGTATGCTT GCTCCAGAAG TTACGGTCTT 
GGAACCAAAC TTCCATGGGA TACTCAATAC CTCATCGAAT CTCTGTCAGA CTCTACAATC 
TACAATGCTT ATTATACAGT TGCTCATCTT CTCCAACAGG GAGCTTTTGA TGGATCAGTC 
GTCGGACCTG CTGGAATTAA AGCTGATCAG ATGACAGATG CTTCGTGGAG TTATGTATTC 
CTCGGAGAAA TTTACGATTC GAAAACCATG CCGGTTGAGG AGGAAAAGCT TAAATCATTG 
AGGAAAGAAT TCATGTATTG GTATCCAATT GATATGAGAG CATCTGGAAA AGATCTTATC 
GGAAATCATT TGACATATCT TCTGTTCAAT CATGCTGCAA TCTGGCCAAC AGACACTTCA 
AAATGGCCAA AAGGAATTCG GGCCAATGGA CATTTGCTCT TGAACAATGA GAAAATGTCG 
AAATCCACTG GAAACTTTAT GACATTGGAA GAAGCCATCG AAAAGTTCAG CGCAGATGGT 
ATGAGATTGA GTCTTGCCGA TGCTGGGGAT GGTTTGGAAG ATGCTAACTT TGTGTACGCA 
ATGGCAGATG CAGCTATTCT GAGACTATTC ACTATGATCG AATGGATCAA GGAAATGATT 
GAACAACGGG ATGCCGGACT TCTCCGAAAA GATGCAAAGA AATTCGCTGA TCGAGTTTTT 
GCAAATGAAA TGAATTCACT GATCGCTGCC ACAGAACAAA ATTATGAAGC TACCAACTTC 
AAGGATGCAC TGAAAACTGG ATTCTTCGAA TATCAAGCCA TTAGAGATAC ATACAGAGAA 
CTTTGCGCTG GAATTGATGA GCCAATGAGC GAATCGCTTG TCTTTAGATT CATTGAAACT 
CAGATGCTCA TCCTATCACC AATCTGCCCA CATATTGCTG AATACATCTG GCAATTGCTC 
GGAAAAGACG GCTTGATTGT AAATGCTCCA TGGCCAACTG TCGATCCTGT CGATGAGAAG 
CTCGCCATTG GTGCTCGTTT CATCACGGAA TCGCTGGCCG AATTCCGTGC CCGACTGAAA 
ACCTACATGA CTCCAAAGAA GAAGGCACTG AAGGAGATTC CAGAAGTTCC AACTGAAGCA 
GTGATCTATG TTGCTAAAGA ATATCCACCA TGGCAGAAGA CTATTCTTGA TATTCTTGAG 
AAACAAGCCA AGGCTAATAA TGGAGCACTT CCAGATAATA AAGCAATCAG TCAGCTCATT 
GGAAAAGAAG AATCACTCAA GAAATTCGCC AAAAAAGCGA TGCCATTCGT TCAAATGATC 
AAGGAAAGAT TCGAACAGAA GGGTGTTTCA GCATTGGCTT CTTCTAGTCC AGTTGATCAA 
ACTTCAATTC TCAACGAGAA TATTGATTTT ATTATGAACG CTTTGGATTT GGATCGTGTT 
ACTATTCGAC ATACTGATGA GGAGGGAATT GATGCGAATA TTGTTGAGAC TACAGTACCG 
CTGGTGCCAA TGTTGAATTT CACGCCGAAT AGACCAACAA TCAAACTTGT CGCCAGAAAT 
GTTCAAATCT GCAATGCAAT GTTCGATGTT GATGTACCAA TTGTTAATGG AGACAGTGTT 
TCAATGGTTA TTCGGAAGAT GAGACGCATC AGCAAGGCAA TCAAACCGAA ATTCGAAGTG 
AGCCTCTGGA GATACAAGAA CGCGGTTTGG GGAGATCGAC AGATGATTTC GTACAGAAAT 
CCATTCGAGG AGAACATTCA ATTGTCCGAC GCAGACATTT TCAATTTCGA GGCTGATAAC 
AAGATTTCTG TGACTTCTGG AAGTGAAAAG TTCGATCTTG GGCGTACTAT TGTGTACAAG 
GCTAACGTCC CAGAAAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_497837.1
CDS	1..3561
Translation	MSKINKERKKVAQLQEIEKSIQELWESKKAFEADARDDGKPKYLVTFPFPYMNGRLHLGHTFSASKCEFAAGFQRLQGKEVLFPFGFHCTGMPIKACADKLKREMQDFGYPPNFPEDVVEEVKEEVSAVDEIIKDKSKGKKSKLVAKTGNAKYQWQIMKSLGLCDEEIKEFSDPNHWLYYFPPHCIADLKKMGLKADWRRSFITTDVNPYFDSFVRWQFNLLRAAKKIDFGKRYTIYSPKDGQPCMDHDRASGEGVGPQEYTLIKLKVLDPKPQALSHIKEDIYLVAATLRPETMYGQTNCYLHPDIQYSVFYATENEKQVFVATARSARIMSYQGLTKENGKVNYVLGLEKIAGSKILGAPLSAPLAKYERVYALPMLTIKDDKGTGVVTSVPSDSPDDFAALSDLKKKKPLREKYGLTDEMVLPFEPVPIIKIEGLGDLAAVEMCSRLKIESQNEKDKLEEAKKEVYLKGFYDGVMLVGKYAGKKTADVKKVIQDDLTAEGLATKYVEPEKKVMSRSGDECVVALCDQWYLNYGEAEWKAAAKKVLEPLRTFNDETRRGLETTVDWLHEYACSRSYGLGTKLPWDTQYLIESLSDSTIYNAYYTVAHLLQQGAFDGSVVGPAGIKADQMTDASWSYVFLGEIYDSKTMPVEEEKLKSLRKEFMYWYPIDMRASGKDLIGNHLTYLLFNHAAIWPTDTSKWPKGIRANGHLLLNNEKMSKSTGNFMTLEEAIEKFSADGMRLSLADAGDGLEDANFVYAMADAAILRLFTMIEWIKEMIEQRDAGLLRKDAKKFADRVFANEMNSLIAATEQNYEATNFKDALKTGFFEYQAIRDTYRELCAGIDEPMSESLVFRFIETQMLILSPICPHIAEYIWQLLGKDGLIVNAPWPTVDPVDEKLAIGARFITESLAEFRARLKTYMTPKKKALKEIPEVPTEAVIYVAKEYPPWQKTILDILEKQAKANNGALPDNKAISQLIGKEESLKKFAKKAMPFVQMIKERFEQKGVSALASSSPVDQTSILNENIDFIMNALDLDRVTIRHTDEEGIDANIVETTVPLVPMLNFTPNRPTIKLVARNVQICNAMFDVDVPIVNGDSVSMVIRKMRRISKAIKPKFEVSLWRYKNAVWGDRQMISYRNPFEENIQLSDADIFNFEADNKISVTSGSEKFDLGRTIVYKANVPEN

Target ORF information:

RefSeq Version	NM_065436.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Leucine--tRNA ligase (lars-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065436.5

ORF Insert Sequence:

ATGTCGAAAA TCAATAAGGA GCGGAAGAAG GTTGCTCAAC TTCAAGAAAT TGAAAAGAGC 
ATTCAGGAGC TGTGGGAGAG CAAGAAGGCT TTCGAGGCGG ATGCTAGGGA TGATGGAAAA 
CCAAAGTACC TCGTCACGTT TCCCTTCCCA TACATGAATG GACGTCTTCA TTTGGGACAC 
ACATTCTCAG CTTCAAAATG TGAATTTGCC GCCGGTTTCC AACGTCTTCA AGGCAAAGAA 
GTGCTCTTTC CATTCGGATT TCATTGTACT GGAATGCCAA TTAAAGCATG TGCAGATAAA 
TTGAAAAGAG AAATGCAAGA TTTCGGATAT CCACCAAATT TCCCGGAAGA TGTTGTCGAG 
GAAGTTAAAG AAGAAGTTTC GGCTGTCGAT GAGATTATCA AGGATAAGAG TAAAGGAAAG 
AAGAGTAAAT TGGTGGCAAA AACTGGAAAT GCAAAATACC AATGGCAAAT TATGAAGTCT 
CTGGGATTGT GTGATGAGGA AATTAAGGAA TTCTCGGATC CAAATCATTG GCTATATTAT 
TTCCCACCAC ATTGCATTGC TGATCTTAAG AAAATGGGAC TAAAGGCTGA TTGGCGTCGT 
TCCTTCATCA CCACCGACGT GAATCCATAC TTCGACTCAT TCGTCCGTTG GCAGTTCAAC 
CTTCTTCGTG CAGCTAAGAA GATCGATTTC GGAAAGAGAT ATACAATTTA TTCACCGAAA 
GACGGTCAAC CATGCATGGA TCACGATCGT GCCAGTGGAG AAGGTGTTGG TCCACAAGAA 
TATACTCTTA TTAAACTGAA AGTTCTTGAT CCAAAACCTC AAGCTTTGAG CCATATCAAA 
GAGGATATTT ATCTCGTTGC GGCTACTTTG AGACCTGAAA CAATGTACGG ACAAACGAAT 
TGTTATCTCC ATCCAGATAT TCAATATTCC GTGTTTTATG CGACGGAAAA CGAGAAACAA 
GTATTTGTGG CAACTGCCAG ATCTGCTAGA ATCATGTCAT ATCAAGGATT GACTAAGGAA 
AATGGTAAAG TCAACTATGT CCTGGGACTC GAGAAAATCG CTGGAAGCAA GATTCTCGGA 
GCTCCACTCT CTGCGCCTCT CGCAAAATAC GAAAGAGTTT ATGCATTGCC AATGTTGACT 
ATCAAGGATG ATAAGGGAAC TGGTGTTGTT ACAAGTGTAC CATCAGACTC TCCAGATGAT 
TTTGCAGCTT TGTCGGATCT CAAGAAGAAG AAGCCATTGA GAGAAAAATA TGGATTGACT 
GATGAGATGG TTCTTCCATT CGAACCAGTT CCAATTATTA AAATCGAAGG ACTTGGGGAT 
TTGGCTGCAG TTGAAATGTG CTCAAGGCTG AAAATTGAGT CTCAAAATGA GAAGGACAAA 
CTGGAAGAGG CGAAGAAAGA AGTTTATTTG AAAGGATTCT ACGATGGAGT TATGCTCGTC 
GGAAAGTATG CTGGAAAGAA GACAGCCGAC GTCAAGAAAG TTATTCAAGA TGATTTGACA 
GCTGAAGGTC TTGCCACGAA ATATGTGGAG CCTGAGAAGA AGGTGATGAG CAGATCTGGA 
GATGAGTGCG TCGTTGCGTT GTGTGATCAA TGGTATTTGA ATTATGGAGA GGCTGAATGG 
AAGGCTGCTG CAAAGAAGGT TCTTGAGCCA CTCAGAACTT TCAATGATGA GACCCGCCGT 
GGATTGGAAA CCACAGTCGA TTGGCTCCAC GAGTATGCTT GCTCCAGAAG TTACGGTCTT 
GGAACCAAAC TTCCATGGGA TACTCAATAC CTCATCGAAT CTCTGTCAGA CTCTACAATC 
TACAATGCTT ATTATACAGT TGCTCATCTT CTCCAACAGG GAGCTTTTGA TGGATCAGTC 
GTCGGACCTG CTGGAATTAA AGCTGATCAG ATGACAGATG CTTCGTGGAG TTATGTATTC 
CTCGGAGAAA TTTACGATTC GAAAACCATG CCGGTTGAGG AGGAAAAGCT TAAATCATTG 
AGGAAAGAAT TCATGTATTG GTATCCAATT GATATGAGAG CATCTGGAAA AGATCTTATC 
GGAAATCATT TGACATATCT TCTGTTCAAT CATGCTGCAA TCTGGCCAAC AGACACTTCA 
AAATGGCCAA AAGGAATTCG GGCCAATGGA CATTTGCTCT TGAACAATGA GAAAATGTCG 
AAATCCACTG GAAACTTTAT GACATTGGAA GAAGCCATCG AAAAGTTCAG CGCAGATGGT 
ATGAGATTGA GTCTTGCCGA TGCTGGGGAT GGTTTGGAAG ATGCTAACTT TGTGTACGCA 
ATGGCAGATG CAGCTATTCT GAGACTATTC ACTATGATCG AATGGATCAA GGAAATGATT 
GAACAACGGG ATGCCGGACT TCTCCGAAAA GATGCAAAGA AATTCGCTGA TCGAGTTTTT 
GCAAATGAAA TGAATTCACT GATCGCTGCC ACAGAACAAA ATTATGAAGC TACCAACTTC 
AAGGATGCAC TGAAAACTGG ATTCTTCGAA TATCAAGCCA TTAGAGATAC ATACAGAGAA 
CTTTGCGCTG GAATTGATGA GCCAATGAGC GAATCGCTTG TCTTTAGATT CATTGAAACT 
CAGATGCTCA TCCTATCACC AATCTGCCCA CATATTGCTG AATACATCTG GCAATTGCTC 
GGAAAAGACG GCTTGATTGT AAATGCTCCA TGGCCAACTG TCGATCCTGT CGATGAGAAG 
CTCGCCATTG GTGCTCGTTT CATCACGGAA TCGCTGGCCG AATTCCGTGC CCGACTGAAA 
ACCTACATGA CTCCAAAGAA GAAGGCACTG AAGGAGATTC CAGAAGTTCC AACTGAAGCA 
GTGATCTATG TTGCTAAAGA ATATCCACCA TGGCAGAAGA CTATTCTTGA TATTCTTGAG 
AAACAAGCCA AGGCTAATAA TGGAGCACTT CCAGATAATA AAGCAATCAG TCAGCTCATT 
GGAAAAGAAG AATCACTCAA GAAATTCGCC AAAAAAGCGA TGCCATTCGT TCAAATGATC 
AAGGAAAGAT TCGAACAGAA GGGTGTTTCA GCATTGGCTT CTTCTAGTCC AGTTGATCAA 
ACTTCAATTC TCAACGAGAA TATTGATTTT ATTATGAACG CTTTGGATTT GGATCGTGTT 
ACTATTCGAC ATACTGATGA GGAGGGAATT GATGCGAATA TTGTTGAGAC TACAGTACCG 
CTGGTGCCAA TGTTGAATTT CACGCCGAAT AGACCAACAA TCAAACTTGT CGCCAGAAAT 
GTTCAAATCT GCAATGCAAT GTTCGATGTT GATGTACCAA TTGTTAATGG AGACAGTGTT 
TCAATGGTTA TTCGGAAGAT GAGACGCATC AGCAAGGCAA TCAAACCGAA ATTCGAAGTG 
AGCCTCTGGA GATACAAGAA CGCGGTTTGG GGAGATCGAC AGATGATTTC GTACAGAAAT 
CCATTCGAGG AGAACATTCA ATTGTCCGAC GCAGACATTT TCAATTTCGA GGCTGATAAC 
AAGATTTCTG TGACTTCTGG AAGTGAAAAG TTCGATCTTG GGCGTACTAT TGTGTACAAG 
GCTAACGTCC CAGAAAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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lars-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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