LARS cDNA ORF clone, Canis lupus familiaris(dog)

The following LARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe286738 XM_022409138.1
Latest version!
Canis lupus familiaris leucyl-tRNA synthetase (LARS), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe03059 XM_535229.6
Latest version!
Canis lupus familiaris leucyl-tRNA synthetase (LARS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe03060 XM_014108236.2
Latest version!
Canis lupus familiaris leucyl-tRNA synthetase (LARS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OCe03059 XM_535229.5 Canis lupus familiaris leucyl-tRNA synthetase (LARS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe03060 XM_014108236.1 Canis lupus familiaris leucyl-tRNA synthetase (LARS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe286738
    Clone ID Related Accession (Same CDS sequence) XM_022409138.1
    Accession Version XM_022409138.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product leucine--tRNA ligase, cytoplasmic isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTATCA AGGCATGTGC TGATAAGTTG AAAAGAGAAA TAGAGCTTTA TGGTTGCCCT 
    CCTGATTTTC CAGATGAAGA AGAGGAAGAG GAAGAAATCC CTGTTAAAAC AGAAGAAGTA
    ATAATTAAAG ATAAAGCTAA AGGAAAAAAG AGTAAGGCTG CTGCTAAAGC TGGATCTTCT
    AAATATCAGT GGGGCATTAT GAAATCCCTT GGTCTGTCTG ATGAAGAGAT AGTTAAATTT
    TCTGAAGCAG AACATTGGCT TGATTATTTC CCACCACTGG CTATTCAGGA TTTGAAGAGA
    ATGGGTTTGA AGGTAGACTG GCGTCGTTCC TTCATCACCA CTGATGTTAA TCCTTACTAT
    GATTCATTTG TCAGGTGGCA GTTTTTAACG TTAAGAGAAA GAAACAAAAT AAAATTTGGG
    AAGCGGTATA CCATTTATTC TCCAAAAGAT GGACAGCCTT GCATGGATCA TGACAGGCAA
    ACTGGAGAGG GTGTGGGACC TCAGGAATAT ACTTTAATCA AGTTGAAAGT GCTTGAGCCG
    TACCCATCCA AATTAAGTGG CCTGAAAGGG AAAAACATCT TCTTGGTAGC TGCTACTCTC
    AGACCTGAGA CCATGTTTGG ACAGACCAAC TGTTGGGTTC GCCCTGATAT GAAATACATT
    GGGTTTGAGA CAGTGAATGG TGATATATTC ATTTGCACCC AAAGAGCTGC CAGGAATATG
    TCATACCAGG GCTTTACCAA ACACAATGGA GTGGTGCCTG TTGTTAAGGA ATTAATGGGA
    GAGGAAATTC TTGGTGCATC ACTTTCTGCA CCTTTAACAT CTTATACGGT GGTCTATGTT
    CTTCCAATGC TCACCATTAA GGAGGATAAA GGTACTGGCG TGGTCACAAG TGTTCCTTCT
    GACTCCCCCG ATGATTTTGC TGCCCTCAGA GATCTGAAGA AAAAGCAAGC CTTACGAGCA
    AAGTATGGAA TTAGAGATGA CATGGTCTTG CCATTTGAGC CGGTGCCAAT CATCGAAATC
    CCAGGTTTTG GAAATCTTTC TGCTGTAACC ATTTGTGATG ATTTGAAAGT TCAAAGCCAG
    AATGACCGAG AAAAACTTGC AGAAGCAAAG GAGAAGTTAT ATTTAAAAGG GTTTTATGAT
    GGTATAATGT TGGTGGATGG ATTTAAAGGA CAGAAGGTCC AAGATGTAAA GAAGACTATT
    CAGAAAAAGA TGATTGACAC TGGAGATGCA TTTACTTACA TGGAACCAGA GAAACAAGTT
    ATGTCCAGAT CTTCAGATGA ATGTGTTGTG GCGCTGTGCG ACCAGTGGTA CTTGGATTAT
    GGAGAAGAAA ATTGGAAAAA ACAGACATCC CAGTGCTTGA AAGGCCTGGA AACATTCTGT
    GAGGAGACCA GGAGGAATTT TGAAGCTACC TTAGGTTGGC TACAAGAGCA TGCCTGCTCA
    AGAACTTACG GTTTAGGTAC TCGTCTGCCG TGGGATGAGC AGTGGCTGAT TGAATCGCTC
    TCTGATTCCA CTATTTACAT GGCATTTTAC ACAGTTGCAC ACCTCCTGCA GGGGGGTAAC
    TTGCGTGGAC AGACAGAGTC TCCACTTGGT ATCAGACCGC AACAGATGAC CAGGGAGGTT
    TGGGATTATG TTTTCTTCAA GGAGGCTCCA TTTCCTAAGA CTCAGATCCC AAAGGAAAAA
    TTAGATCAGT TAAAGCAGGA ATTTGAGTTC TGGTATCCTG TGGATCTCCG AGTCTCTGGA
    AAGGATCTTG TTCCAAATCA TCTCTCATAT TACCTTTATA ATCATGTGGC AATGTGGCCA
    GAACAAAGTG ACAAATGGCC TGCTGCTGTG AGAGCAAATG GACATCTCCT CCTCAACTCT
    GAGAAGATGT CAAAGTCCAC AGGCAACTTC CTCACTTTGA CCCAAGCTAT CGACAAATTT
    TCAGCTGATG GAATGCGTTT GGCTCTGGCT GATGCTGGCG ACACTGTTGA AGATGCAAAC
    TTTGTGGAAG CCATGGCAGA TGCAGGTATT CTCCGTCTGT ACACCTGGGT GGAGTGGGTG
    AAAGAAATGG TTGCCAACTG GGACAGCCTA AGAAGTGGGC CTGCCAGCAC CTTCAATGAT
    AGAGTTTTTG CCAGTGAGAT GAATGCAGGA ATTATAAAAA CAGATCAAAA TTATGAAAAG
    ATGATGTTTA AAGAAGCCCT GAAAACAGGG TTTTTTGAGT TTCAGGCTGC GAAAGATAAA
    TACCGTGAAT TGGCCATAGA AGGTATGCAC AGAGACCTTG TGTTCCGCTT TATTGAAGTT
    CAAACACTTC TCTTGGCTCC ATTCTGTCCA CACCTATGTG AACACATCTG GACACTCCTG
    GGAAAGCCTG ACTCCATTAT GAATGCTTCA TGGCCTCTGG CTGGTCCTGT GGATGAAGCC
    TTGATACGTT CCTCACAGTA TCTCATGGAA GTAGCACATG ACCTTAGATT GCGACTCAAG
    AACTATATGA TGCCAGCTAA GGGGAAGAAG ACTGACAAGC AACCACCTCA GAAACCTTCA
    CATTGCACCA TCTATGTGGC GAAGAACTAT CCTTCTTGGC AACATACCAC CCTGTCAGTG
    CTGCGTAATC ACTTTGAGGC CAACAGTGGA AAATTGCCTG ATAACAAAGT CATTGCTAGT
    GAACTGGGCA ATCTGCCAGA ATTGAAGAAA TATATGAAGA AAGTGATGCC GTTTGTTGCC
    ATGATTAAGG AAAATCTGGA GAAGATGGGA CCTCGTGTTC TGGATTTGCA GCTAGAATTT
    GATGAACAGG CGGTGCTGAT GGAGAATTTA GTTTACCTGA CCAATTCCCT TGAGCTAGAA
    CACATAGAAG TCAAGTTTGC CTCCGAAGCA GAAGATAAAG TCAGGGAAGA CTGCTGTCCT
    GGGAAACCAC TTAATGTTTT CAGGACAGAA CCTGGTGTGT CAGTCTCCCT AGTGAATCCC
    CAGCCATCAA ATGGCCACTT CACAACCAAA ATTGAAATCA GGCAAGGGGA TAACCGCGAT
    TCTATAATCA GGCGTTTAAT GAAAATGGAC CGAGGCATCA AAGACCTTTC TAAAGTGAAG
    CTGATGAGAT TTGATGATCC ACTGTTGGGA CCTCGGCGAG TTCCTGTCCT GGGAAAAGAG
    CACACTGGGA AGACTCCCAT TTCTGAGCAT GCTGTTTTCC ATGTGGACCT CATGAGCAAG
    AAAATTCATC TCACTGAAAA TGGGCTAGGG GCAGATATTG GTGATACAAT TATCTATTTG
    GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022264846.1
    CDS159..3407
    Translation

    Target ORF information:

    RefSeq Version XM_022409138.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris leucyl-tRNA synthetase (LARS), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022409138.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTATCA AGGCATGTGC TGATAAGTTG AAAAGAGAAA TAGAGCTTTA TGGTTGCCCT 
    CCTGATTTTC CAGATGAAGA AGAGGAAGAG GAAGAAATCC CTGTTAAAAC AGAAGAAGTA
    ATAATTAAAG ATAAAGCTAA AGGAAAAAAG AGTAAGGCTG CTGCTAAAGC TGGATCTTCT
    AAATATCAGT GGGGCATTAT GAAATCCCTT GGTCTGTCTG ATGAAGAGAT AGTTAAATTT
    TCTGAAGCAG AACATTGGCT TGATTATTTC CCACCACTGG CTATTCAGGA TTTGAAGAGA
    ATGGGTTTGA AGGTAGACTG GCGTCGTTCC TTCATCACCA CTGATGTTAA TCCTTACTAT
    GATTCATTTG TCAGGTGGCA GTTTTTAACG TTAAGAGAAA GAAACAAAAT AAAATTTGGG
    AAGCGGTATA CCATTTATTC TCCAAAAGAT GGACAGCCTT GCATGGATCA TGACAGGCAA
    ACTGGAGAGG GTGTGGGACC TCAGGAATAT ACTTTAATCA AGTTGAAAGT GCTTGAGCCG
    TACCCATCCA AATTAAGTGG CCTGAAAGGG AAAAACATCT TCTTGGTAGC TGCTACTCTC
    AGACCTGAGA CCATGTTTGG ACAGACCAAC TGTTGGGTTC GCCCTGATAT GAAATACATT
    GGGTTTGAGA CAGTGAATGG TGATATATTC ATTTGCACCC AAAGAGCTGC CAGGAATATG
    TCATACCAGG GCTTTACCAA ACACAATGGA GTGGTGCCTG TTGTTAAGGA ATTAATGGGA
    GAGGAAATTC TTGGTGCATC ACTTTCTGCA CCTTTAACAT CTTATACGGT GGTCTATGTT
    CTTCCAATGC TCACCATTAA GGAGGATAAA GGTACTGGCG TGGTCACAAG TGTTCCTTCT
    GACTCCCCCG ATGATTTTGC TGCCCTCAGA GATCTGAAGA AAAAGCAAGC CTTACGAGCA
    AAGTATGGAA TTAGAGATGA CATGGTCTTG CCATTTGAGC CGGTGCCAAT CATCGAAATC
    CCAGGTTTTG GAAATCTTTC TGCTGTAACC ATTTGTGATG ATTTGAAAGT TCAAAGCCAG
    AATGACCGAG AAAAACTTGC AGAAGCAAAG GAGAAGTTAT ATTTAAAAGG GTTTTATGAT
    GGTATAATGT TGGTGGATGG ATTTAAAGGA CAGAAGGTCC AAGATGTAAA GAAGACTATT
    CAGAAAAAGA TGATTGACAC TGGAGATGCA TTTACTTACA TGGAACCAGA GAAACAAGTT
    ATGTCCAGAT CTTCAGATGA ATGTGTTGTG GCGCTGTGCG ACCAGTGGTA CTTGGATTAT
    GGAGAAGAAA ATTGGAAAAA ACAGACATCC CAGTGCTTGA AAGGCCTGGA AACATTCTGT
    GAGGAGACCA GGAGGAATTT TGAAGCTACC TTAGGTTGGC TACAAGAGCA TGCCTGCTCA
    AGAACTTACG GTTTAGGTAC TCGTCTGCCG TGGGATGAGC AGTGGCTGAT TGAATCGCTC
    TCTGATTCCA CTATTTACAT GGCATTTTAC ACAGTTGCAC ACCTCCTGCA GGGGGGTAAC
    TTGCGTGGAC AGACAGAGTC TCCACTTGGT ATCAGACCGC AACAGATGAC CAGGGAGGTT
    TGGGATTATG TTTTCTTCAA GGAGGCTCCA TTTCCTAAGA CTCAGATCCC AAAGGAAAAA
    TTAGATCAGT TAAAGCAGGA ATTTGAGTTC TGGTATCCTG TGGATCTCCG AGTCTCTGGA
    AAGGATCTTG TTCCAAATCA TCTCTCATAT TACCTTTATA ATCATGTGGC AATGTGGCCA
    GAACAAAGTG ACAAATGGCC TGCTGCTGTG AGAGCAAATG GACATCTCCT CCTCAACTCT
    GAGAAGATGT CAAAGTCCAC AGGCAACTTC CTCACTTTGA CCCAAGCTAT CGACAAATTT
    TCAGCTGATG GAATGCGTTT GGCTCTGGCT GATGCTGGCG ACACTGTTGA AGATGCAAAC
    TTTGTGGAAG CCATGGCAGA TGCAGGTATT CTCCGTCTGT ACACCTGGGT GGAGTGGGTG
    AAAGAAATGG TTGCCAACTG GGACAGCCTA AGAAGTGGGC CTGCCAGCAC CTTCAATGAT
    AGAGTTTTTG CCAGTGAGAT GAATGCAGGA ATTATAAAAA CAGATCAAAA TTATGAAAAG
    ATGATGTTTA AAGAAGCCCT GAAAACAGGG TTTTTTGAGT TTCAGGCTGC GAAAGATAAA
    TACCGTGAAT TGGCCATAGA AGGTATGCAC AGAGACCTTG TGTTCCGCTT TATTGAAGTT
    CAAACACTTC TCTTGGCTCC ATTCTGTCCA CACCTATGTG AACACATCTG GACACTCCTG
    GGAAAGCCTG ACTCCATTAT GAATGCTTCA TGGCCTCTGG CTGGTCCTGT GGATGAAGCC
    TTGATACGTT CCTCACAGTA TCTCATGGAA GTAGCACATG ACCTTAGATT GCGACTCAAG
    AACTATATGA TGCCAGCTAA GGGGAAGAAG ACTGACAAGC AACCACCTCA GAAACCTTCA
    CATTGCACCA TCTATGTGGC GAAGAACTAT CCTTCTTGGC AACATACCAC CCTGTCAGTG
    CTGCGTAATC ACTTTGAGGC CAACAGTGGA AAATTGCCTG ATAACAAAGT CATTGCTAGT
    GAACTGGGCA ATCTGCCAGA ATTGAAGAAA TATATGAAGA AAGTGATGCC GTTTGTTGCC
    ATGATTAAGG AAAATCTGGA GAAGATGGGA CCTCGTGTTC TGGATTTGCA GCTAGAATTT
    GATGAACAGG CGGTGCTGAT GGAGAATTTA GTTTACCTGA CCAATTCCCT TGAGCTAGAA
    CACATAGAAG TCAAGTTTGC CTCCGAAGCA GAAGATAAAG TCAGGGAAGA CTGCTGTCCT
    GGGAAACCAC TTAATGTTTT CAGGACAGAA CCTGGTGTGT CAGTCTCCCT AGTGAATCCC
    CAGCCATCAA ATGGCCACTT CACAACCAAA ATTGAAATCA GGCAAGGGGA TAACCGCGAT
    TCTATAATCA GGCGTTTAAT GAAAATGGAC CGAGGCATCA AAGACCTTTC TAAAGTGAAG
    CTGATGAGAT TTGATGATCC ACTGTTGGGA CCTCGGCGAG TTCCTGTCCT GGGAAAAGAG
    CACACTGGGA AGACTCCCAT TTCTGAGCAT GCTGTTTTCC ATGTGGACCT CATGAGCAAG
    AAAATTCATC TCACTGAAAA TGGGCTAGGG GCAGATATTG GTGATACAAT TATCTATTTG
    GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03059
    Clone ID Related Accession (Same CDS sequence) XM_535229.5 , XM_535229.6
    Accession Version XM_535229.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product leucine--tRNA ligase, cytoplasmic isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_535229.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGGAAA GAAAAGGAAC AGCCAAAGTA GACTTTTTGA AGAAGATTGA GAAGGAAATC 
    CAACAGAAAT GGGCTACTGA GAAAGTATTT GAGGTTGATG CATCTAATTT AGAGAACCAG
    ACTGGCAAGG GCAAGTACTT TGTAACCTTC CCATATCCGT ATATGAATGG GCGCCTTCAT
    TTGGGACACA CATTTTCTTT ATCCAAATGT GAGTTTGCAG TAGGATACCA GAGACTGAAA
    GGAAAAACTT GTCTGTTTCC CTTTGGTTTA CACTGTACTG GAATGCCTAT CAAGGCATGT
    GCTGATAAGT TGAAAAGAGA AATAGAGCTT TATGGTTGCC CTCCTGATTT TCCAGATGAA
    GAAGAGGAAG AGGAAGAAAT CCCTGTTAAA ACAGAAGAAG TAATAATTAA AGATAAAGCT
    AAAGGAAAAA AGAGTAAGGC TGCTGCTAAA GCTGGATCTT CTAAATATCA GTGGGGCATT
    ATGAAATCCC TTGGTCTGTC TGATGAAGAG ATAGTTAAAT TTTCTGAAGC AGAACATTGG
    CTTGATTATT TCCCACCACT GGCTATTCAG GATTTGAAGA GAATGGGTTT GAAGGTAGAC
    TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGGTGG
    CAGTTTTTAA CGTTAAGAGA AAGAAACAAA ATAAAATTTG GGAAGCGGTA TACCATTTAT
    TCTCCAAAAG ATGGACAGCC TTGCATGGAT CATGACAGGC AAACTGGAGA GGGTGTGGGA
    CCTCAGGAAT ATACTTTAAT CAAGTTGAAA GTGCTTGAGC CGTACCCATC CAAATTAAGT
    GGCCTGAAAG GGAAAAACAT CTTCTTGGTA GCTGCTACTC TCAGACCTGA GACCATGTTT
    GGACAGACCA ACTGTTGGGT TCGCCCTGAT ATGAAATACA TTGGGTTTGA GACAGTGAAT
    GGTGATATAT TCATTTGCAC CCAAAGAGCT GCCAGGAATA TGTCATACCA GGGCTTTACC
    AAACACAATG GAGTGGTGCC TGTTGTTAAG GAATTAATGG GAGAGGAAAT TCTTGGTGCA
    TCACTTTCTG CACCTTTAAC ATCTTATACG GTGGTCTATG TTCTTCCAAT GCTCACCATT
    AAGGAGGATA AAGGTACTGG CGTGGTCACA AGTGTTCCTT CTGACTCCCC CGATGATTTT
    GCTGCCCTCA GAGATCTGAA GAAAAAGCAA GCCTTACGAG CAAAGTATGG AATTAGAGAT
    GACATGGTCT TGCCATTTGA GCCGGTGCCA ATCATCGAAA TCCCAGGTTT TGGAAATCTT
    TCTGCTGTAA CCATTTGTGA TGATTTGAAA GTTCAAAGCC AGAATGACCG AGAAAAACTT
    GCAGAAGCAA AGGAGAAGTT ATATTTAAAA GGGTTTTATG ATGGTATAAT GTTGGTGGAT
    GGATTTAAAG GACAGAAGGT CCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC
    ACTGGAGATG CATTTACTTA CATGGAACCA GAGAAACAAG TTATGTCCAG ATCTTCAGAT
    GAATGTGTTG TGGCGCTGTG CGACCAGTGG TACTTGGATT ATGGAGAAGA AAATTGGAAA
    AAACAGACAT CCCAGTGCTT GAAAGGCCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT
    TTTGAAGCTA CCTTAGGTTG GCTACAAGAG CATGCCTGCT CAAGAACTTA CGGTTTAGGT
    ACTCGTCTGC CGTGGGATGA GCAGTGGCTG ATTGAATCGC TCTCTGATTC CACTATTTAC
    ATGGCATTTT ACACAGTTGC ACACCTCCTG CAGGGGGGTA ACTTGCGTGG ACAGACAGAG
    TCTCCACTTG GTATCAGACC GCAACAGATG ACCAGGGAGG TTTGGGATTA TGTTTTCTTC
    AAGGAGGCTC CATTTCCTAA GACTCAGATC CCAAAGGAAA AATTAGATCA GTTAAAGCAG
    GAATTTGAGT TCTGGTATCC TGTGGATCTC CGAGTCTCTG GAAAGGATCT TGTTCCAAAT
    CATCTCTCAT ATTACCTTTA TAATCATGTG GCAATGTGGC CAGAACAAAG TGACAAATGG
    CCTGCTGCTG TGAGAGCAAA TGGACATCTC CTCCTCAACT CTGAGAAGAT GTCAAAGTCC
    ACAGGCAACT TCCTCACTTT GACCCAAGCT ATCGACAAAT TTTCAGCTGA TGGAATGCGT
    TTGGCTCTGG CTGATGCTGG CGACACTGTT GAAGATGCAA ACTTTGTGGA AGCCATGGCA
    GATGCAGGTA TTCTCCGTCT GTACACCTGG GTGGAGTGGG TGAAAGAAAT GGTTGCCAAC
    TGGGACAGCC TAAGAAGTGG GCCTGCCAGC ACCTTCAATG ATAGAGTTTT TGCCAGTGAG
    ATGAATGCAG GAATTATAAA AACAGATCAA AATTATGAAA AGATGATGTT TAAAGAAGCC
    CTGAAAACAG GGTTTTTTGA GTTTCAGGCT GCGAAAGATA AATACCGTGA ATTGGCCATA
    GAAGGTATGC ACAGAGACCT TGTGTTCCGC TTTATTGAAG TTCAAACACT TCTCTTGGCT
    CCATTCTGTC CACACCTATG TGAACACATC TGGACACTCC TGGGAAAGCC TGACTCCATT
    ATGAATGCTT CATGGCCTCT GGCTGGTCCT GTGGATGAAG CCTTGATACG TTCCTCACAG
    TATCTCATGG AAGTAGCACA TGACCTTAGA TTGCGACTCA AGAACTATAT GATGCCAGCT
    AAGGGGAAGA AGACTGACAA GCAACCACCT CAGAAACCTT CACATTGCAC CATCTATGTG
    GCGAAGAACT ATCCTTCTTG GCAACATACC ACCCTGTCAG TGCTGCGTAA TCACTTTGAG
    GCCAACAGTG GAAAATTGCC TGATAACAAA GTCATTGCTA GTGAACTGGG CAATCTGCCA
    GAATTGAAGA AATATATGAA GAAAGTGATG CCGTTTGTTG CCATGATTAA GGAAAATCTG
    GAGAAGATGG GACCTCGTGT TCTGGATTTG CAGCTAGAAT TTGATGAACA GGCGGTGCTG
    ATGGAGAATT TAGTTTACCT GACCAATTCC CTTGAGCTAG AACACATAGA AGTCAAGTTT
    GCCTCCGAAG CAGAAGATAA AGTCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT
    TTCAGGACAG AACCTGGTGT GTCAGTCTCC CTAGTGAATC CCCAGCCATC AAATGGCCAC
    TTCACAACCA AAATTGAAAT CAGGCAAGGG GATAACCGCG ATTCTATAAT CAGGCGTTTA
    ATGAAAATGG ACCGAGGCAT CAAAGACCTT TCTAAAGTGA AGCTGATGAG ATTTGATGAT
    CCACTGTTGG GACCTCGGCG AGTTCCTGTC CTGGGAAAAG AGCACACTGG GAAGACTCCC
    ATTTCTGAGC ATGCTGTTTT CCATGTGGAC CTCATGAGCA AGAAAATTCA TCTCACTGAA
    AATGGGCTAG GGGCAGATAT TGGTGATACA ATTATCTATT TGGTTCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_535229.3
    CDS174..3704
    Translation

    Target ORF information:

    RefSeq Version XM_535229.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris leucyl-tRNA synthetase (LARS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_535229.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGGAAA GAAAAGGAAC AGCCAAAGTA GACTTTTTGA AGAAGATTGA GAAGGAAATC 
    CAACAGAAAT GGGCTACTGA GAAAGTATTT GAGGTTGATG CATCTAATTT AGAGAACCAG
    ACTGGCAAGG GCAAGTACTT TGTAACCTTC CCATATCCGT ATATGAATGG GCGCCTTCAT
    TTGGGACACA CATTTTCTTT ATCCAAATGT GAGTTTGCAG TAGGATACCA GAGACTGAAA
    GGAAAAACTT GTCTGTTTCC CTTTGGTTTA CACTGTACTG GAATGCCTAT CAAGGCATGT
    GCTGATAAGT TGAAAAGAGA AATAGAGCTT TATGGTTGCC CTCCTGATTT TCCAGATGAA
    GAAGAGGAAG AGGAAGAAAT CCCTGTTAAA ACAGAAGAAG TAATAATTAA AGATAAAGCT
    AAAGGAAAAA AGAGTAAGGC TGCTGCTAAA GCTGGATCTT CTAAATATCA GTGGGGCATT
    ATGAAATCCC TTGGTCTGTC TGATGAAGAG ATAGTTAAAT TTTCTGAAGC AGAACATTGG
    CTTGATTATT TCCCACCACT GGCTATTCAG GATTTGAAGA GAATGGGTTT GAAGGTAGAC
    TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGGTGG
    CAGTTTTTAA CGTTAAGAGA AAGAAACAAA ATAAAATTTG GGAAGCGGTA TACCATTTAT
    TCTCCAAAAG ATGGACAGCC TTGCATGGAT CATGACAGGC AAACTGGAGA GGGTGTGGGA
    CCTCAGGAAT ATACTTTAAT CAAGTTGAAA GTGCTTGAGC CGTACCCATC CAAATTAAGT
    GGCCTGAAAG GGAAAAACAT CTTCTTGGTA GCTGCTACTC TCAGACCTGA GACCATGTTT
    GGACAGACCA ACTGTTGGGT TCGCCCTGAT ATGAAATACA TTGGGTTTGA GACAGTGAAT
    GGTGATATAT TCATTTGCAC CCAAAGAGCT GCCAGGAATA TGTCATACCA GGGCTTTACC
    AAACACAATG GAGTGGTGCC TGTTGTTAAG GAATTAATGG GAGAGGAAAT TCTTGGTGCA
    TCACTTTCTG CACCTTTAAC ATCTTATACG GTGGTCTATG TTCTTCCAAT GCTCACCATT
    AAGGAGGATA AAGGTACTGG CGTGGTCACA AGTGTTCCTT CTGACTCCCC CGATGATTTT
    GCTGCCCTCA GAGATCTGAA GAAAAAGCAA GCCTTACGAG CAAAGTATGG AATTAGAGAT
    GACATGGTCT TGCCATTTGA GCCGGTGCCA ATCATCGAAA TCCCAGGTTT TGGAAATCTT
    TCTGCTGTAA CCATTTGTGA TGATTTGAAA GTTCAAAGCC AGAATGACCG AGAAAAACTT
    GCAGAAGCAA AGGAGAAGTT ATATTTAAAA GGGTTTTATG ATGGTATAAT GTTGGTGGAT
    GGATTTAAAG GACAGAAGGT CCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC
    ACTGGAGATG CATTTACTTA CATGGAACCA GAGAAACAAG TTATGTCCAG ATCTTCAGAT
    GAATGTGTTG TGGCGCTGTG CGACCAGTGG TACTTGGATT ATGGAGAAGA AAATTGGAAA
    AAACAGACAT CCCAGTGCTT GAAAGGCCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT
    TTTGAAGCTA CCTTAGGTTG GCTACAAGAG CATGCCTGCT CAAGAACTTA CGGTTTAGGT
    ACTCGTCTGC CGTGGGATGA GCAGTGGCTG ATTGAATCGC TCTCTGATTC CACTATTTAC
    ATGGCATTTT ACACAGTTGC ACACCTCCTG CAGGGGGGTA ACTTGCGTGG ACAGACAGAG
    TCTCCACTTG GTATCAGACC GCAACAGATG ACCAGGGAGG TTTGGGATTA TGTTTTCTTC
    AAGGAGGCTC CATTTCCTAA GACTCAGATC CCAAAGGAAA AATTAGATCA GTTAAAGCAG
    GAATTTGAGT TCTGGTATCC TGTGGATCTC CGAGTCTCTG GAAAGGATCT TGTTCCAAAT
    CATCTCTCAT ATTACCTTTA TAATCATGTG GCAATGTGGC CAGAACAAAG TGACAAATGG
    CCTGCTGCTG TGAGAGCAAA TGGACATCTC CTCCTCAACT CTGAGAAGAT GTCAAAGTCC
    ACAGGCAACT TCCTCACTTT GACCCAAGCT ATCGACAAAT TTTCAGCTGA TGGAATGCGT
    TTGGCTCTGG CTGATGCTGG CGACACTGTT GAAGATGCAA ACTTTGTGGA AGCCATGGCA
    GATGCAGGTA TTCTCCGTCT GTACACCTGG GTGGAGTGGG TGAAAGAAAT GGTTGCCAAC
    TGGGACAGCC TAAGAAGTGG GCCTGCCAGC ACCTTCAATG ATAGAGTTTT TGCCAGTGAG
    ATGAATGCAG GAATTATAAA AACAGATCAA AATTATGAAA AGATGATGTT TAAAGAAGCC
    CTGAAAACAG GGTTTTTTGA GTTTCAGGCT GCGAAAGATA AATACCGTGA ATTGGCCATA
    GAAGGTATGC ACAGAGACCT TGTGTTCCGC TTTATTGAAG TTCAAACACT TCTCTTGGCT
    CCATTCTGTC CACACCTATG TGAACACATC TGGACACTCC TGGGAAAGCC TGACTCCATT
    ATGAATGCTT CATGGCCTCT GGCTGGTCCT GTGGATGAAG CCTTGATACG TTCCTCACAG
    TATCTCATGG AAGTAGCACA TGACCTTAGA TTGCGACTCA AGAACTATAT GATGCCAGCT
    AAGGGGAAGA AGACTGACAA GCAACCACCT CAGAAACCTT CACATTGCAC CATCTATGTG
    GCGAAGAACT ATCCTTCTTG GCAACATACC ACCCTGTCAG TGCTGCGTAA TCACTTTGAG
    GCCAACAGTG GAAAATTGCC TGATAACAAA GTCATTGCTA GTGAACTGGG CAATCTGCCA
    GAATTGAAGA AATATATGAA GAAAGTGATG CCGTTTGTTG CCATGATTAA GGAAAATCTG
    GAGAAGATGG GACCTCGTGT TCTGGATTTG CAGCTAGAAT TTGATGAACA GGCGGTGCTG
    ATGGAGAATT TAGTTTACCT GACCAATTCC CTTGAGCTAG AACACATAGA AGTCAAGTTT
    GCCTCCGAAG CAGAAGATAA AGTCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT
    TTCAGGACAG AACCTGGTGT GTCAGTCTCC CTAGTGAATC CCCAGCCATC AAATGGCCAC
    TTCACAACCA AAATTGAAAT CAGGCAAGGG GATAACCGCG ATTCTATAAT CAGGCGTTTA
    ATGAAAATGG ACCGAGGCAT CAAAGACCTT TCTAAAGTGA AGCTGATGAG ATTTGATGAT
    CCACTGTTGG GACCTCGGCG AGTTCCTGTC CTGGGAAAAG AGCACACTGG GAAGACTCCC
    ATTTCTGAGC ATGCTGTTTT CCATGTGGAC CTCATGAGCA AGAAAATTCA TCTCACTGAA
    AATGGGCTAG GGGCAGATAT TGGTGATACA ATTATCTATT TGGTTCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03059
    Clone ID Related Accession (Same CDS sequence) XM_535229.5 , XM_535229.6
    Accession Version XM_535229.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product leucine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_535229.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGGAAA GAAAAGGAAC AGCCAAAGTA GACTTTTTGA AGAAGATTGA GAAGGAAATC 
    CAACAGAAAT GGGCTACTGA GAAAGTATTT GAGGTTGATG CATCTAATTT AGAGAACCAG
    ACTGGCAAGG GCAAGTACTT TGTAACCTTC CCATATCCGT ATATGAATGG GCGCCTTCAT
    TTGGGACACA CATTTTCTTT ATCCAAATGT GAGTTTGCAG TAGGATACCA GAGACTGAAA
    GGAAAAACTT GTCTGTTTCC CTTTGGTTTA CACTGTACTG GAATGCCTAT CAAGGCATGT
    GCTGATAAGT TGAAAAGAGA AATAGAGCTT TATGGTTGCC CTCCTGATTT TCCAGATGAA
    GAAGAGGAAG AGGAAGAAAT CCCTGTTAAA ACAGAAGAAG TAATAATTAA AGATAAAGCT
    AAAGGAAAAA AGAGTAAGGC TGCTGCTAAA GCTGGATCTT CTAAATATCA GTGGGGCATT
    ATGAAATCCC TTGGTCTGTC TGATGAAGAG ATAGTTAAAT TTTCTGAAGC AGAACATTGG
    CTTGATTATT TCCCACCACT GGCTATTCAG GATTTGAAGA GAATGGGTTT GAAGGTAGAC
    TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGGTGG
    CAGTTTTTAA CGTTAAGAGA AAGAAACAAA ATAAAATTTG GGAAGCGGTA TACCATTTAT
    TCTCCAAAAG ATGGACAGCC TTGCATGGAT CATGACAGGC AAACTGGAGA GGGTGTGGGA
    CCTCAGGAAT ATACTTTAAT CAAGTTGAAA GTGCTTGAGC CGTACCCATC CAAATTAAGT
    GGCCTGAAAG GGAAAAACAT CTTCTTGGTA GCTGCTACTC TCAGACCTGA GACCATGTTT
    GGACAGACCA ACTGTTGGGT TCGCCCTGAT ATGAAATACA TTGGGTTTGA GACAGTGAAT
    GGTGATATAT TCATTTGCAC CCAAAGAGCT GCCAGGAATA TGTCATACCA GGGCTTTACC
    AAACACAATG GAGTGGTGCC TGTTGTTAAG GAATTAATGG GAGAGGAAAT TCTTGGTGCA
    TCACTTTCTG CACCTTTAAC ATCTTATACG GTGGTCTATG TTCTTCCAAT GCTCACCATT
    AAGGAGGATA AAGGTACTGG CGTGGTCACA AGTGTTCCTT CTGACTCCCC CGATGATTTT
    GCTGCCCTCA GAGATCTGAA GAAAAAGCAA GCCTTACGAG CAAAGTATGG AATTAGAGAT
    GACATGGTCT TGCCATTTGA GCCGGTGCCA ATCATCGAAA TCCCAGGTTT TGGAAATCTT
    TCTGCTGTAA CCATTTGTGA TGATTTGAAA GTTCAAAGCC AGAATGACCG AGAAAAACTT
    GCAGAAGCAA AGGAGAAGTT ATATTTAAAA GGGTTTTATG ATGGTATAAT GTTGGTGGAT
    GGATTTAAAG GACAGAAGGT CCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC
    ACTGGAGATG CATTTACTTA CATGGAACCA GAGAAACAAG TTATGTCCAG ATCTTCAGAT
    GAATGTGTTG TGGCGCTGTG CGACCAGTGG TACTTGGATT ATGGAGAAGA AAATTGGAAA
    AAACAGACAT CCCAGTGCTT GAAAGGCCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT
    TTTGAAGCTA CCTTAGGTTG GCTACAAGAG CATGCCTGCT CAAGAACTTA CGGTTTAGGT
    ACTCGTCTGC CGTGGGATGA GCAGTGGCTG ATTGAATCGC TCTCTGATTC CACTATTTAC
    ATGGCATTTT ACACAGTTGC ACACCTCCTG CAGGGGGGTA ACTTGCGTGG ACAGACAGAG
    TCTCCACTTG GTATCAGACC GCAACAGATG ACCAGGGAGG TTTGGGATTA TGTTTTCTTC
    AAGGAGGCTC CATTTCCTAA GACTCAGATC CCAAAGGAAA AATTAGATCA GTTAAAGCAG
    GAATTTGAGT TCTGGTATCC TGTGGATCTC CGAGTCTCTG GAAAGGATCT TGTTCCAAAT
    CATCTCTCAT ATTACCTTTA TAATCATGTG GCAATGTGGC CAGAACAAAG TGACAAATGG
    CCTGCTGCTG TGAGAGCAAA TGGACATCTC CTCCTCAACT CTGAGAAGAT GTCAAAGTCC
    ACAGGCAACT TCCTCACTTT GACCCAAGCT ATCGACAAAT TTTCAGCTGA TGGAATGCGT
    TTGGCTCTGG CTGATGCTGG CGACACTGTT GAAGATGCAA ACTTTGTGGA AGCCATGGCA
    GATGCAGGTA TTCTCCGTCT GTACACCTGG GTGGAGTGGG TGAAAGAAAT GGTTGCCAAC
    TGGGACAGCC TAAGAAGTGG GCCTGCCAGC ACCTTCAATG ATAGAGTTTT TGCCAGTGAG
    ATGAATGCAG GAATTATAAA AACAGATCAA AATTATGAAA AGATGATGTT TAAAGAAGCC
    CTGAAAACAG GGTTTTTTGA GTTTCAGGCT GCGAAAGATA AATACCGTGA ATTGGCCATA
    GAAGGTATGC ACAGAGACCT TGTGTTCCGC TTTATTGAAG TTCAAACACT TCTCTTGGCT
    CCATTCTGTC CACACCTATG TGAACACATC TGGACACTCC TGGGAAAGCC TGACTCCATT
    ATGAATGCTT CATGGCCTCT GGCTGGTCCT GTGGATGAAG CCTTGATACG TTCCTCACAG
    TATCTCATGG AAGTAGCACA TGACCTTAGA TTGCGACTCA AGAACTATAT GATGCCAGCT
    AAGGGGAAGA AGACTGACAA GCAACCACCT CAGAAACCTT CACATTGCAC CATCTATGTG
    GCGAAGAACT ATCCTTCTTG GCAACATACC ACCCTGTCAG TGCTGCGTAA TCACTTTGAG
    GCCAACAGTG GAAAATTGCC TGATAACAAA GTCATTGCTA GTGAACTGGG CAATCTGCCA
    GAATTGAAGA AATATATGAA GAAAGTGATG CCGTTTGTTG CCATGATTAA GGAAAATCTG
    GAGAAGATGG GACCTCGTGT TCTGGATTTG CAGCTAGAAT TTGATGAACA GGCGGTGCTG
    ATGGAGAATT TAGTTTACCT GACCAATTCC CTTGAGCTAG AACACATAGA AGTCAAGTTT
    GCCTCCGAAG CAGAAGATAA AGTCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT
    TTCAGGACAG AACCTGGTGT GTCAGTCTCC CTAGTGAATC CCCAGCCATC AAATGGCCAC
    TTCACAACCA AAATTGAAAT CAGGCAAGGG GATAACCGCG ATTCTATAAT CAGGCGTTTA
    ATGAAAATGG ACCGAGGCAT CAAAGACCTT TCTAAAGTGA AGCTGATGAG ATTTGATGAT
    CCACTGTTGG GACCTCGGCG AGTTCCTGTC CTGGGAAAAG AGCACACTGG GAAGACTCCC
    ATTTCTGAGC ATGCTGTTTT CCATGTGGAC CTCATGAGCA AGAAAATTCA TCTCACTGAA
    AATGGGCTAG GGGCAGATAT TGGTGATACA ATTATCTATT TGGTTCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_535229.3
    CDS175..3705
    Translation

    Target ORF information:

    RefSeq Version XM_535229.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris leucyl-tRNA synthetase (LARS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_535229.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGGAAA GAAAAGGAAC AGCCAAAGTA GACTTTTTGA AGAAGATTGA GAAGGAAATC 
    CAACAGAAAT GGGCTACTGA GAAAGTATTT GAGGTTGATG CATCTAATTT AGAGAACCAG
    ACTGGCAAGG GCAAGTACTT TGTAACCTTC CCATATCCGT ATATGAATGG GCGCCTTCAT
    TTGGGACACA CATTTTCTTT ATCCAAATGT GAGTTTGCAG TAGGATACCA GAGACTGAAA
    GGAAAAACTT GTCTGTTTCC CTTTGGTTTA CACTGTACTG GAATGCCTAT CAAGGCATGT
    GCTGATAAGT TGAAAAGAGA AATAGAGCTT TATGGTTGCC CTCCTGATTT TCCAGATGAA
    GAAGAGGAAG AGGAAGAAAT CCCTGTTAAA ACAGAAGAAG TAATAATTAA AGATAAAGCT
    AAAGGAAAAA AGAGTAAGGC TGCTGCTAAA GCTGGATCTT CTAAATATCA GTGGGGCATT
    ATGAAATCCC TTGGTCTGTC TGATGAAGAG ATAGTTAAAT TTTCTGAAGC AGAACATTGG
    CTTGATTATT TCCCACCACT GGCTATTCAG GATTTGAAGA GAATGGGTTT GAAGGTAGAC
    TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGGTGG
    CAGTTTTTAA CGTTAAGAGA AAGAAACAAA ATAAAATTTG GGAAGCGGTA TACCATTTAT
    TCTCCAAAAG ATGGACAGCC TTGCATGGAT CATGACAGGC AAACTGGAGA GGGTGTGGGA
    CCTCAGGAAT ATACTTTAAT CAAGTTGAAA GTGCTTGAGC CGTACCCATC CAAATTAAGT
    GGCCTGAAAG GGAAAAACAT CTTCTTGGTA GCTGCTACTC TCAGACCTGA GACCATGTTT
    GGACAGACCA ACTGTTGGGT TCGCCCTGAT ATGAAATACA TTGGGTTTGA GACAGTGAAT
    GGTGATATAT TCATTTGCAC CCAAAGAGCT GCCAGGAATA TGTCATACCA GGGCTTTACC
    AAACACAATG GAGTGGTGCC TGTTGTTAAG GAATTAATGG GAGAGGAAAT TCTTGGTGCA
    TCACTTTCTG CACCTTTAAC ATCTTATACG GTGGTCTATG TTCTTCCAAT GCTCACCATT
    AAGGAGGATA AAGGTACTGG CGTGGTCACA AGTGTTCCTT CTGACTCCCC CGATGATTTT
    GCTGCCCTCA GAGATCTGAA GAAAAAGCAA GCCTTACGAG CAAAGTATGG AATTAGAGAT
    GACATGGTCT TGCCATTTGA GCCGGTGCCA ATCATCGAAA TCCCAGGTTT TGGAAATCTT
    TCTGCTGTAA CCATTTGTGA TGATTTGAAA GTTCAAAGCC AGAATGACCG AGAAAAACTT
    GCAGAAGCAA AGGAGAAGTT ATATTTAAAA GGGTTTTATG ATGGTATAAT GTTGGTGGAT
    GGATTTAAAG GACAGAAGGT CCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC
    ACTGGAGATG CATTTACTTA CATGGAACCA GAGAAACAAG TTATGTCCAG ATCTTCAGAT
    GAATGTGTTG TGGCGCTGTG CGACCAGTGG TACTTGGATT ATGGAGAAGA AAATTGGAAA
    AAACAGACAT CCCAGTGCTT GAAAGGCCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT
    TTTGAAGCTA CCTTAGGTTG GCTACAAGAG CATGCCTGCT CAAGAACTTA CGGTTTAGGT
    ACTCGTCTGC CGTGGGATGA GCAGTGGCTG ATTGAATCGC TCTCTGATTC CACTATTTAC
    ATGGCATTTT ACACAGTTGC ACACCTCCTG CAGGGGGGTA ACTTGCGTGG ACAGACAGAG
    TCTCCACTTG GTATCAGACC GCAACAGATG ACCAGGGAGG TTTGGGATTA TGTTTTCTTC
    AAGGAGGCTC CATTTCCTAA GACTCAGATC CCAAAGGAAA AATTAGATCA GTTAAAGCAG
    GAATTTGAGT TCTGGTATCC TGTGGATCTC CGAGTCTCTG GAAAGGATCT TGTTCCAAAT
    CATCTCTCAT ATTACCTTTA TAATCATGTG GCAATGTGGC CAGAACAAAG TGACAAATGG
    CCTGCTGCTG TGAGAGCAAA TGGACATCTC CTCCTCAACT CTGAGAAGAT GTCAAAGTCC
    ACAGGCAACT TCCTCACTTT GACCCAAGCT ATCGACAAAT TTTCAGCTGA TGGAATGCGT
    TTGGCTCTGG CTGATGCTGG CGACACTGTT GAAGATGCAA ACTTTGTGGA AGCCATGGCA
    GATGCAGGTA TTCTCCGTCT GTACACCTGG GTGGAGTGGG TGAAAGAAAT GGTTGCCAAC
    TGGGACAGCC TAAGAAGTGG GCCTGCCAGC ACCTTCAATG ATAGAGTTTT TGCCAGTGAG
    ATGAATGCAG GAATTATAAA AACAGATCAA AATTATGAAA AGATGATGTT TAAAGAAGCC
    CTGAAAACAG GGTTTTTTGA GTTTCAGGCT GCGAAAGATA AATACCGTGA ATTGGCCATA
    GAAGGTATGC ACAGAGACCT TGTGTTCCGC TTTATTGAAG TTCAAACACT TCTCTTGGCT
    CCATTCTGTC CACACCTATG TGAACACATC TGGACACTCC TGGGAAAGCC TGACTCCATT
    ATGAATGCTT CATGGCCTCT GGCTGGTCCT GTGGATGAAG CCTTGATACG TTCCTCACAG
    TATCTCATGG AAGTAGCACA TGACCTTAGA TTGCGACTCA AGAACTATAT GATGCCAGCT
    AAGGGGAAGA AGACTGACAA GCAACCACCT CAGAAACCTT CACATTGCAC CATCTATGTG
    GCGAAGAACT ATCCTTCTTG GCAACATACC ACCCTGTCAG TGCTGCGTAA TCACTTTGAG
    GCCAACAGTG GAAAATTGCC TGATAACAAA GTCATTGCTA GTGAACTGGG CAATCTGCCA
    GAATTGAAGA AATATATGAA GAAAGTGATG CCGTTTGTTG CCATGATTAA GGAAAATCTG
    GAGAAGATGG GACCTCGTGT TCTGGATTTG CAGCTAGAAT TTGATGAACA GGCGGTGCTG
    ATGGAGAATT TAGTTTACCT GACCAATTCC CTTGAGCTAG AACACATAGA AGTCAAGTTT
    GCCTCCGAAG CAGAAGATAA AGTCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT
    TTCAGGACAG AACCTGGTGT GTCAGTCTCC CTAGTGAATC CCCAGCCATC AAATGGCCAC
    TTCACAACCA AAATTGAAAT CAGGCAAGGG GATAACCGCG ATTCTATAAT CAGGCGTTTA
    ATGAAAATGG ACCGAGGCAT CAAAGACCTT TCTAAAGTGA AGCTGATGAG ATTTGATGAT
    CCACTGTTGG GACCTCGGCG AGTTCCTGTC CTGGGAAAAG AGCACACTGG GAAGACTCCC
    ATTTCTGAGC ATGCTGTTTT CCATGTGGAC CTCATGAGCA AGAAAATTCA TCTCACTGAA
    AATGGGCTAG GGGCAGATAT TGGTGATACA ATTATCTATT TGGTTCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03060
    Clone ID Related Accession (Same CDS sequence) XM_014108236.1 , XM_014108236.2
    Accession Version XM_014108236.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product leucine--tRNA ligase, cytoplasmic isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAAGAA AAGGAACAGC CAAAGTAGAC TTTTTGAAGA AGATTGAGAA GGAAATCCAA 
    CAGAAATGGG CTACTGAGAA AGTATTTGAG GTTGATGCAT CTAATTTAGA GAACCAGACT
    GGCAAGGGCA AGTACTTTGT AACCTTCCCA TATCCGTATA TGAATGGGCG CCTTCATTTG
    GGACACACAT TTTCTTTATC CAAATGTGAG TTTGCAGTAG GATACCAGAG ACTGAAAGGA
    AAAACTTGTC TGTTTCCCTT TGGTTTACAC TGTACTGGAA TGCCTATCAA GGCATGTGCT
    GATAAGTTGA AAAGAGAAAT AGAGCTTTAT GGTTGCCCTC CTGATTTTCC AGATGAAGAA
    GAGGAAGAGG AAGAAATCCC TGTTAAAACA GAAGAAGTAA TAATTAAAGA TAAAGCTAAA
    GGAAAAAAGA GTAAGGCTGC TGCTAAAGCT GGATCTTCTA AATATCAGTG GGGCATTATG
    AAATCCCTTG GTCTGTCTGA TGAAGAGATA GTTAAATTTT CTGAAGCAGA ACATTGGCTT
    GATTATTTCC CACCACTGGC TATTCAGGAT TTGAAGAGAA TGGGTTTGAA GGTAGACTGG
    CGTCGTTCCT TCATCACCAC TGATGTTAAT CCTTACTATG ATTCATTTGT CAGGTGGCAG
    TTTTTAACGT TAAGAGAAAG AAACAAAATA AAATTTGGGA AGCGGTATAC CATTTATTCT
    CCAAAAGATG GACAGCCTTG CATGGATCAT GACAGGCAAA CTGGAGAGGG TGTGGGACCT
    CAGGAATATA CTTTAATCAA GTTGAAAGTG CTTGAGCCGT ACCCATCCAA ATTAAGTGGC
    CTGAAAGGGA AAAACATCTT CTTGGTAGCT GCTACTCTCA GACCTGAGAC CATGTTTGGA
    CAGACCAACT GTTGGGTTCG CCCTGATATG AAATACATTG GGTTTGAGAC AGTGAATGGT
    GATATATTCA TTTGCACCCA AAGAGCTGCC AGGAATATGT CATACCAGGG CTTTACCAAA
    CACAATGGAG TGGTGCCTGT TGTTAAGGAA TTAATGGGAG AGGAAATTCT TGGTGCATCA
    CTTTCTGCAC CTTTAACATC TTATACGGTG GTCTATGTTC TTCCAATGCT CACCATTAAG
    GAGGATAAAG GTACTGGCGT GGTCACAAGT GTTCCTTCTG ACTCCCCCGA TGATTTTGCT
    GCCCTCAGAG ATCTGAAGAA AAAGCAAGCC TTACGAGCAA AGTATGGAAT TAGAGATGAC
    ATGGTCTTGC CATTTGAGCC GGTGCCAATC ATCGAAATCC CAGGTTTTGG AAATCTTTCT
    GCTGTAACCA TTTGTGATGA TTTGAAAGTT CAAAGCCAGA ATGACCGAGA AAAACTTGCA
    GAAGCAAAGG AGAAGTTATA TTTAAAAGGG TTTTATGATG GTATAATGTT GGTGGATGGA
    TTTAAAGGAC AGAAGGTCCA AGATGTAAAG AAGACTATTC AGAAAAAGAT GATTGACACT
    GGAGATGCAT TTACTTACAT GGAACCAGAG AAACAAGTTA TGTCCAGATC TTCAGATGAA
    TGTGTTGTGG CGCTGTGCGA CCAGTGGTAC TTGGATTATG GAGAAGAAAA TTGGAAAAAA
    CAGACATCCC AGTGCTTGAA AGGCCTGGAA ACATTCTGTG AGGAGACCAG GAGGAATTTT
    GAAGCTACCT TAGGTTGGCT ACAAGAGCAT GCCTGCTCAA GAACTTACGG TTTAGGTACT
    CGTCTGCCGT GGGATGAGCA GTGGCTGATT GAATCGCTCT CTGATTCCAC TATTTACATG
    GCATTTTACA CAGTTGCACA CCTCCTGCAG GGGGGTAACT TGCGTGGACA GACAGAGTCT
    CCACTTGGTA TCAGACCGCA ACAGATGACC AGGGAGGTTT GGGATTATGT TTTCTTCAAG
    GAGGCTCCAT TTCCTAAGAC TCAGATCCCA AAGGAAAAAT TAGATCAGTT AAAGCAGGAA
    TTTGAGTTCT GGTATCCTGT GGATCTCCGA GTCTCTGGAA AGGATCTTGT TCCAAATCAT
    CTCTCATATT ACCTTTATAA TCATGTGGCA ATGTGGCCAG AACAAAGTGA CAAATGGCCT
    GCTGCTGTGA GAGCAAATGG ACATCTCCTC CTCAACTCTG AGAAGATGTC AAAGTCCACA
    GGCAACTTCC TCACTTTGAC CCAAGCTATC GACAAATTTT CAGCTGATGG AATGCGTTTG
    GCTCTGGCTG ATGCTGGCGA CACTGTTGAA GATGCAAACT TTGTGGAAGC CATGGCAGAT
    GCAGGTATTC TCCGTCTGTA CACCTGGGTG GAGTGGGTGA AAGAAATGGT TGCCAACTGG
    GACAGCCTAA GAAGTGGGCC TGCCAGCACC TTCAATGATA GAGTTTTTGC CAGTGAGATG
    AATGCAGGAA TTATAAAAAC AGATCAAAAT TATGAAAAGA TGATGTTTAA AGAAGCCCTG
    AAAACAGGGT TTTTTGAGTT TCAGGCTGCG AAAGATAAAT ACCGTGAATT GGCCATAGAA
    GGTATGCACA GAGACCTTGT GTTCCGCTTT ATTGAAGTTC AAACACTTCT CTTGGCTCCA
    TTCTGTCCAC ACCTATGTGA ACACATCTGG ACACTCCTGG GAAAGCCTGA CTCCATTATG
    AATGCTTCAT GGCCTCTGGC TGGTCCTGTG GATGAAGCCT TGATACGTTC CTCACAGTAT
    CTCATGGAAG TAGCACATGA CCTTAGATTG CGACTCAAGA ACTATATGAT GCCAGCTAAG
    GGGAAGAAGA CTGACAAGCA ACCACCTCAG AAACCTTCAC ATTGCACCAT CTATGTGGCG
    AAGAACTATC CTTCTTGGCA ACATACCACC CTGTCAGTGC TGCGTAATCA CTTTGAGGCC
    AACAGTGGAA AATTGCCTGA TAACAAAGTC ATTGCTAGTG AACTGGGCAA TCTGCCAGAA
    TTGAAGAAAT ATATGAAGAA AGTGATGCCG TTTGTTGCCA TGATTAAGGA AAATCTGGAG
    AAGATGGGAC CTCGTGTTCT GGATTTGCAG CTAGAATTTG ATGAACAGGC GGTGCTGATG
    GAGAATTTAG TTTACCTGAC CAATTCCCTT GAGCTAGAAC ACATAGAAGT CAAGTTTGCC
    TCCGAAGCAG AAGATAAAGT CAGGGAAGAC TGCTGTCCTG GGAAACCACT TAATGTTTTC
    AGGACAGAAC CTGGTGTGTC AGTCTCCCTA GTGAATCCCC AGCCATCAAA TGGCCACTTC
    ACAACCAAAA TTGAAATCAG GCAAGGGGAT AACCGCGATT CTATAATCAG GCGTTTAATG
    AAAATGGACC GAGGCATCAA AGACCTTTCT AAAGTGAAGC TGATGAGATT TGATGATCCA
    CTGTTGGGAC CTCGGCGAGT TCCTGTCCTG GGAAAAGAGC ACACTGGGAA GACTCCCATT
    TCTGAGCATG CTGTTTTCCA TGTGGACCTC ATGAGCAAGA AAATTCATCT CACTGAAAAT
    GGGCTAGGGG CAGATATTGG TGATACAATT ATCTATTTGG TTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963711.1
    CDS333..3860
    Translation

    Target ORF information:

    RefSeq Version XM_014108236.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris leucyl-tRNA synthetase (LARS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108236.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAAGAA AAGGAACAGC CAAAGTAGAC TTTTTGAAGA AGATTGAGAA GGAAATCCAA 
    CAGAAATGGG CTACTGAGAA AGTATTTGAG GTTGATGCAT CTAATTTAGA GAACCAGACT
    GGCAAGGGCA AGTACTTTGT AACCTTCCCA TATCCGTATA TGAATGGGCG CCTTCATTTG
    GGACACACAT TTTCTTTATC CAAATGTGAG TTTGCAGTAG GATACCAGAG ACTGAAAGGA
    AAAACTTGTC TGTTTCCCTT TGGTTTACAC TGTACTGGAA TGCCTATCAA GGCATGTGCT
    GATAAGTTGA AAAGAGAAAT AGAGCTTTAT GGTTGCCCTC CTGATTTTCC AGATGAAGAA
    GAGGAAGAGG AAGAAATCCC TGTTAAAACA GAAGAAGTAA TAATTAAAGA TAAAGCTAAA
    GGAAAAAAGA GTAAGGCTGC TGCTAAAGCT GGATCTTCTA AATATCAGTG GGGCATTATG
    AAATCCCTTG GTCTGTCTGA TGAAGAGATA GTTAAATTTT CTGAAGCAGA ACATTGGCTT
    GATTATTTCC CACCACTGGC TATTCAGGAT TTGAAGAGAA TGGGTTTGAA GGTAGACTGG
    CGTCGTTCCT TCATCACCAC TGATGTTAAT CCTTACTATG ATTCATTTGT CAGGTGGCAG
    TTTTTAACGT TAAGAGAAAG AAACAAAATA AAATTTGGGA AGCGGTATAC CATTTATTCT
    CCAAAAGATG GACAGCCTTG CATGGATCAT GACAGGCAAA CTGGAGAGGG TGTGGGACCT
    CAGGAATATA CTTTAATCAA GTTGAAAGTG CTTGAGCCGT ACCCATCCAA ATTAAGTGGC
    CTGAAAGGGA AAAACATCTT CTTGGTAGCT GCTACTCTCA GACCTGAGAC CATGTTTGGA
    CAGACCAACT GTTGGGTTCG CCCTGATATG AAATACATTG GGTTTGAGAC AGTGAATGGT
    GATATATTCA TTTGCACCCA AAGAGCTGCC AGGAATATGT CATACCAGGG CTTTACCAAA
    CACAATGGAG TGGTGCCTGT TGTTAAGGAA TTAATGGGAG AGGAAATTCT TGGTGCATCA
    CTTTCTGCAC CTTTAACATC TTATACGGTG GTCTATGTTC TTCCAATGCT CACCATTAAG
    GAGGATAAAG GTACTGGCGT GGTCACAAGT GTTCCTTCTG ACTCCCCCGA TGATTTTGCT
    GCCCTCAGAG ATCTGAAGAA AAAGCAAGCC TTACGAGCAA AGTATGGAAT TAGAGATGAC
    ATGGTCTTGC CATTTGAGCC GGTGCCAATC ATCGAAATCC CAGGTTTTGG AAATCTTTCT
    GCTGTAACCA TTTGTGATGA TTTGAAAGTT CAAAGCCAGA ATGACCGAGA AAAACTTGCA
    GAAGCAAAGG AGAAGTTATA TTTAAAAGGG TTTTATGATG GTATAATGTT GGTGGATGGA
    TTTAAAGGAC AGAAGGTCCA AGATGTAAAG AAGACTATTC AGAAAAAGAT GATTGACACT
    GGAGATGCAT TTACTTACAT GGAACCAGAG AAACAAGTTA TGTCCAGATC TTCAGATGAA
    TGTGTTGTGG CGCTGTGCGA CCAGTGGTAC TTGGATTATG GAGAAGAAAA TTGGAAAAAA
    CAGACATCCC AGTGCTTGAA AGGCCTGGAA ACATTCTGTG AGGAGACCAG GAGGAATTTT
    GAAGCTACCT TAGGTTGGCT ACAAGAGCAT GCCTGCTCAA GAACTTACGG TTTAGGTACT
    CGTCTGCCGT GGGATGAGCA GTGGCTGATT GAATCGCTCT CTGATTCCAC TATTTACATG
    GCATTTTACA CAGTTGCACA CCTCCTGCAG GGGGGTAACT TGCGTGGACA GACAGAGTCT
    CCACTTGGTA TCAGACCGCA ACAGATGACC AGGGAGGTTT GGGATTATGT TTTCTTCAAG
    GAGGCTCCAT TTCCTAAGAC TCAGATCCCA AAGGAAAAAT TAGATCAGTT AAAGCAGGAA
    TTTGAGTTCT GGTATCCTGT GGATCTCCGA GTCTCTGGAA AGGATCTTGT TCCAAATCAT
    CTCTCATATT ACCTTTATAA TCATGTGGCA ATGTGGCCAG AACAAAGTGA CAAATGGCCT
    GCTGCTGTGA GAGCAAATGG ACATCTCCTC CTCAACTCTG AGAAGATGTC AAAGTCCACA
    GGCAACTTCC TCACTTTGAC CCAAGCTATC GACAAATTTT CAGCTGATGG AATGCGTTTG
    GCTCTGGCTG ATGCTGGCGA CACTGTTGAA GATGCAAACT TTGTGGAAGC CATGGCAGAT
    GCAGGTATTC TCCGTCTGTA CACCTGGGTG GAGTGGGTGA AAGAAATGGT TGCCAACTGG
    GACAGCCTAA GAAGTGGGCC TGCCAGCACC TTCAATGATA GAGTTTTTGC CAGTGAGATG
    AATGCAGGAA TTATAAAAAC AGATCAAAAT TATGAAAAGA TGATGTTTAA AGAAGCCCTG
    AAAACAGGGT TTTTTGAGTT TCAGGCTGCG AAAGATAAAT ACCGTGAATT GGCCATAGAA
    GGTATGCACA GAGACCTTGT GTTCCGCTTT ATTGAAGTTC AAACACTTCT CTTGGCTCCA
    TTCTGTCCAC ACCTATGTGA ACACATCTGG ACACTCCTGG GAAAGCCTGA CTCCATTATG
    AATGCTTCAT GGCCTCTGGC TGGTCCTGTG GATGAAGCCT TGATACGTTC CTCACAGTAT
    CTCATGGAAG TAGCACATGA CCTTAGATTG CGACTCAAGA ACTATATGAT GCCAGCTAAG
    GGGAAGAAGA CTGACAAGCA ACCACCTCAG AAACCTTCAC ATTGCACCAT CTATGTGGCG
    AAGAACTATC CTTCTTGGCA ACATACCACC CTGTCAGTGC TGCGTAATCA CTTTGAGGCC
    AACAGTGGAA AATTGCCTGA TAACAAAGTC ATTGCTAGTG AACTGGGCAA TCTGCCAGAA
    TTGAAGAAAT ATATGAAGAA AGTGATGCCG TTTGTTGCCA TGATTAAGGA AAATCTGGAG
    AAGATGGGAC CTCGTGTTCT GGATTTGCAG CTAGAATTTG ATGAACAGGC GGTGCTGATG
    GAGAATTTAG TTTACCTGAC CAATTCCCTT GAGCTAGAAC ACATAGAAGT CAAGTTTGCC
    TCCGAAGCAG AAGATAAAGT CAGGGAAGAC TGCTGTCCTG GGAAACCACT TAATGTTTTC
    AGGACAGAAC CTGGTGTGTC AGTCTCCCTA GTGAATCCCC AGCCATCAAA TGGCCACTTC
    ACAACCAAAA TTGAAATCAG GCAAGGGGAT AACCGCGATT CTATAATCAG GCGTTTAATG
    AAAATGGACC GAGGCATCAA AGACCTTTCT AAAGTGAAGC TGATGAGATT TGATGATCCA
    CTGTTGGGAC CTCGGCGAGT TCCTGTCCTG GGAAAAGAGC ACACTGGGAA GACTCCCATT
    TCTGAGCATG CTGTTTTCCA TGTGGACCTC ATGAGCAAGA AAATTCATCT CACTGAAAAT
    GGGCTAGGGG CAGATATTGG TGATACAATT ATCTATTTGG TTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03060
    Clone ID Related Accession (Same CDS sequence) XM_014108236.1 , XM_014108236.2
    Accession Version XM_014108236.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product leucine--tRNA ligase, cytoplasmic isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108236.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAAGAA AAGGAACAGC CAAAGTAGAC TTTTTGAAGA AGATTGAGAA GGAAATCCAA 
    CAGAAATGGG CTACTGAGAA AGTATTTGAG GTTGATGCAT CTAATTTAGA GAACCAGACT
    GGCAAGGGCA AGTACTTTGT AACCTTCCCA TATCCGTATA TGAATGGGCG CCTTCATTTG
    GGACACACAT TTTCTTTATC CAAATGTGAG TTTGCAGTAG GATACCAGAG ACTGAAAGGA
    AAAACTTGTC TGTTTCCCTT TGGTTTACAC TGTACTGGAA TGCCTATCAA GGCATGTGCT
    GATAAGTTGA AAAGAGAAAT AGAGCTTTAT GGTTGCCCTC CTGATTTTCC AGATGAAGAA
    GAGGAAGAGG AAGAAATCCC TGTTAAAACA GAAGAAGTAA TAATTAAAGA TAAAGCTAAA
    GGAAAAAAGA GTAAGGCTGC TGCTAAAGCT GGATCTTCTA AATATCAGTG GGGCATTATG
    AAATCCCTTG GTCTGTCTGA TGAAGAGATA GTTAAATTTT CTGAAGCAGA ACATTGGCTT
    GATTATTTCC CACCACTGGC TATTCAGGAT TTGAAGAGAA TGGGTTTGAA GGTAGACTGG
    CGTCGTTCCT TCATCACCAC TGATGTTAAT CCTTACTATG ATTCATTTGT CAGGTGGCAG
    TTTTTAACGT TAAGAGAAAG AAACAAAATA AAATTTGGGA AGCGGTATAC CATTTATTCT
    CCAAAAGATG GACAGCCTTG CATGGATCAT GACAGGCAAA CTGGAGAGGG TGTGGGACCT
    CAGGAATATA CTTTAATCAA GTTGAAAGTG CTTGAGCCGT ACCCATCCAA ATTAAGTGGC
    CTGAAAGGGA AAAACATCTT CTTGGTAGCT GCTACTCTCA GACCTGAGAC CATGTTTGGA
    CAGACCAACT GTTGGGTTCG CCCTGATATG AAATACATTG GGTTTGAGAC AGTGAATGGT
    GATATATTCA TTTGCACCCA AAGAGCTGCC AGGAATATGT CATACCAGGG CTTTACCAAA
    CACAATGGAG TGGTGCCTGT TGTTAAGGAA TTAATGGGAG AGGAAATTCT TGGTGCATCA
    CTTTCTGCAC CTTTAACATC TTATACGGTG GTCTATGTTC TTCCAATGCT CACCATTAAG
    GAGGATAAAG GTACTGGCGT GGTCACAAGT GTTCCTTCTG ACTCCCCCGA TGATTTTGCT
    GCCCTCAGAG ATCTGAAGAA AAAGCAAGCC TTACGAGCAA AGTATGGAAT TAGAGATGAC
    ATGGTCTTGC CATTTGAGCC GGTGCCAATC ATCGAAATCC CAGGTTTTGG AAATCTTTCT
    GCTGTAACCA TTTGTGATGA TTTGAAAGTT CAAAGCCAGA ATGACCGAGA AAAACTTGCA
    GAAGCAAAGG AGAAGTTATA TTTAAAAGGG TTTTATGATG GTATAATGTT GGTGGATGGA
    TTTAAAGGAC AGAAGGTCCA AGATGTAAAG AAGACTATTC AGAAAAAGAT GATTGACACT
    GGAGATGCAT TTACTTACAT GGAACCAGAG AAACAAGTTA TGTCCAGATC TTCAGATGAA
    TGTGTTGTGG CGCTGTGCGA CCAGTGGTAC TTGGATTATG GAGAAGAAAA TTGGAAAAAA
    CAGACATCCC AGTGCTTGAA AGGCCTGGAA ACATTCTGTG AGGAGACCAG GAGGAATTTT
    GAAGCTACCT TAGGTTGGCT ACAAGAGCAT GCCTGCTCAA GAACTTACGG TTTAGGTACT
    CGTCTGCCGT GGGATGAGCA GTGGCTGATT GAATCGCTCT CTGATTCCAC TATTTACATG
    GCATTTTACA CAGTTGCACA CCTCCTGCAG GGGGGTAACT TGCGTGGACA GACAGAGTCT
    CCACTTGGTA TCAGACCGCA ACAGATGACC AGGGAGGTTT GGGATTATGT TTTCTTCAAG
    GAGGCTCCAT TTCCTAAGAC TCAGATCCCA AAGGAAAAAT TAGATCAGTT AAAGCAGGAA
    TTTGAGTTCT GGTATCCTGT GGATCTCCGA GTCTCTGGAA AGGATCTTGT TCCAAATCAT
    CTCTCATATT ACCTTTATAA TCATGTGGCA ATGTGGCCAG AACAAAGTGA CAAATGGCCT
    GCTGCTGTGA GAGCAAATGG ACATCTCCTC CTCAACTCTG AGAAGATGTC AAAGTCCACA
    GGCAACTTCC TCACTTTGAC CCAAGCTATC GACAAATTTT CAGCTGATGG AATGCGTTTG
    GCTCTGGCTG ATGCTGGCGA CACTGTTGAA GATGCAAACT TTGTGGAAGC CATGGCAGAT
    GCAGGTATTC TCCGTCTGTA CACCTGGGTG GAGTGGGTGA AAGAAATGGT TGCCAACTGG
    GACAGCCTAA GAAGTGGGCC TGCCAGCACC TTCAATGATA GAGTTTTTGC CAGTGAGATG
    AATGCAGGAA TTATAAAAAC AGATCAAAAT TATGAAAAGA TGATGTTTAA AGAAGCCCTG
    AAAACAGGGT TTTTTGAGTT TCAGGCTGCG AAAGATAAAT ACCGTGAATT GGCCATAGAA
    GGTATGCACA GAGACCTTGT GTTCCGCTTT ATTGAAGTTC AAACACTTCT CTTGGCTCCA
    TTCTGTCCAC ACCTATGTGA ACACATCTGG ACACTCCTGG GAAAGCCTGA CTCCATTATG
    AATGCTTCAT GGCCTCTGGC TGGTCCTGTG GATGAAGCCT TGATACGTTC CTCACAGTAT
    CTCATGGAAG TAGCACATGA CCTTAGATTG CGACTCAAGA ACTATATGAT GCCAGCTAAG
    GGGAAGAAGA CTGACAAGCA ACCACCTCAG AAACCTTCAC ATTGCACCAT CTATGTGGCG
    AAGAACTATC CTTCTTGGCA ACATACCACC CTGTCAGTGC TGCGTAATCA CTTTGAGGCC
    AACAGTGGAA AATTGCCTGA TAACAAAGTC ATTGCTAGTG AACTGGGCAA TCTGCCAGAA
    TTGAAGAAAT ATATGAAGAA AGTGATGCCG TTTGTTGCCA TGATTAAGGA AAATCTGGAG
    AAGATGGGAC CTCGTGTTCT GGATTTGCAG CTAGAATTTG ATGAACAGGC GGTGCTGATG
    GAGAATTTAG TTTACCTGAC CAATTCCCTT GAGCTAGAAC ACATAGAAGT CAAGTTTGCC
    TCCGAAGCAG AAGATAAAGT CAGGGAAGAC TGCTGTCCTG GGAAACCACT TAATGTTTTC
    AGGACAGAAC CTGGTGTGTC AGTCTCCCTA GTGAATCCCC AGCCATCAAA TGGCCACTTC
    ACAACCAAAA TTGAAATCAG GCAAGGGGAT AACCGCGATT CTATAATCAG GCGTTTAATG
    AAAATGGACC GAGGCATCAA AGACCTTTCT AAAGTGAAGC TGATGAGATT TGATGATCCA
    CTGTTGGGAC CTCGGCGAGT TCCTGTCCTG GGAAAAGAGC ACACTGGGAA GACTCCCATT
    TCTGAGCATG CTGTTTTCCA TGTGGACCTC ATGAGCAAGA AAATTCATCT CACTGAAAAT
    GGGCTAGGGG CAGATATTGG TGATACAATT ATCTATTTGG TTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963711.1
    CDS334..3861
    Translation

    Target ORF information:

    RefSeq Version XM_014108236.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris leucyl-tRNA synthetase (LARS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108236.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAAGAA AAGGAACAGC CAAAGTAGAC TTTTTGAAGA AGATTGAGAA GGAAATCCAA 
    CAGAAATGGG CTACTGAGAA AGTATTTGAG GTTGATGCAT CTAATTTAGA GAACCAGACT
    GGCAAGGGCA AGTACTTTGT AACCTTCCCA TATCCGTATA TGAATGGGCG CCTTCATTTG
    GGACACACAT TTTCTTTATC CAAATGTGAG TTTGCAGTAG GATACCAGAG ACTGAAAGGA
    AAAACTTGTC TGTTTCCCTT TGGTTTACAC TGTACTGGAA TGCCTATCAA GGCATGTGCT
    GATAAGTTGA AAAGAGAAAT AGAGCTTTAT GGTTGCCCTC CTGATTTTCC AGATGAAGAA
    GAGGAAGAGG AAGAAATCCC TGTTAAAACA GAAGAAGTAA TAATTAAAGA TAAAGCTAAA
    GGAAAAAAGA GTAAGGCTGC TGCTAAAGCT GGATCTTCTA AATATCAGTG GGGCATTATG
    AAATCCCTTG GTCTGTCTGA TGAAGAGATA GTTAAATTTT CTGAAGCAGA ACATTGGCTT
    GATTATTTCC CACCACTGGC TATTCAGGAT TTGAAGAGAA TGGGTTTGAA GGTAGACTGG
    CGTCGTTCCT TCATCACCAC TGATGTTAAT CCTTACTATG ATTCATTTGT CAGGTGGCAG
    TTTTTAACGT TAAGAGAAAG AAACAAAATA AAATTTGGGA AGCGGTATAC CATTTATTCT
    CCAAAAGATG GACAGCCTTG CATGGATCAT GACAGGCAAA CTGGAGAGGG TGTGGGACCT
    CAGGAATATA CTTTAATCAA GTTGAAAGTG CTTGAGCCGT ACCCATCCAA ATTAAGTGGC
    CTGAAAGGGA AAAACATCTT CTTGGTAGCT GCTACTCTCA GACCTGAGAC CATGTTTGGA
    CAGACCAACT GTTGGGTTCG CCCTGATATG AAATACATTG GGTTTGAGAC AGTGAATGGT
    GATATATTCA TTTGCACCCA AAGAGCTGCC AGGAATATGT CATACCAGGG CTTTACCAAA
    CACAATGGAG TGGTGCCTGT TGTTAAGGAA TTAATGGGAG AGGAAATTCT TGGTGCATCA
    CTTTCTGCAC CTTTAACATC TTATACGGTG GTCTATGTTC TTCCAATGCT CACCATTAAG
    GAGGATAAAG GTACTGGCGT GGTCACAAGT GTTCCTTCTG ACTCCCCCGA TGATTTTGCT
    GCCCTCAGAG ATCTGAAGAA AAAGCAAGCC TTACGAGCAA AGTATGGAAT TAGAGATGAC
    ATGGTCTTGC CATTTGAGCC GGTGCCAATC ATCGAAATCC CAGGTTTTGG AAATCTTTCT
    GCTGTAACCA TTTGTGATGA TTTGAAAGTT CAAAGCCAGA ATGACCGAGA AAAACTTGCA
    GAAGCAAAGG AGAAGTTATA TTTAAAAGGG TTTTATGATG GTATAATGTT GGTGGATGGA
    TTTAAAGGAC AGAAGGTCCA AGATGTAAAG AAGACTATTC AGAAAAAGAT GATTGACACT
    GGAGATGCAT TTACTTACAT GGAACCAGAG AAACAAGTTA TGTCCAGATC TTCAGATGAA
    TGTGTTGTGG CGCTGTGCGA CCAGTGGTAC TTGGATTATG GAGAAGAAAA TTGGAAAAAA
    CAGACATCCC AGTGCTTGAA AGGCCTGGAA ACATTCTGTG AGGAGACCAG GAGGAATTTT
    GAAGCTACCT TAGGTTGGCT ACAAGAGCAT GCCTGCTCAA GAACTTACGG TTTAGGTACT
    CGTCTGCCGT GGGATGAGCA GTGGCTGATT GAATCGCTCT CTGATTCCAC TATTTACATG
    GCATTTTACA CAGTTGCACA CCTCCTGCAG GGGGGTAACT TGCGTGGACA GACAGAGTCT
    CCACTTGGTA TCAGACCGCA ACAGATGACC AGGGAGGTTT GGGATTATGT TTTCTTCAAG
    GAGGCTCCAT TTCCTAAGAC TCAGATCCCA AAGGAAAAAT TAGATCAGTT AAAGCAGGAA
    TTTGAGTTCT GGTATCCTGT GGATCTCCGA GTCTCTGGAA AGGATCTTGT TCCAAATCAT
    CTCTCATATT ACCTTTATAA TCATGTGGCA ATGTGGCCAG AACAAAGTGA CAAATGGCCT
    GCTGCTGTGA GAGCAAATGG ACATCTCCTC CTCAACTCTG AGAAGATGTC AAAGTCCACA
    GGCAACTTCC TCACTTTGAC CCAAGCTATC GACAAATTTT CAGCTGATGG AATGCGTTTG
    GCTCTGGCTG ATGCTGGCGA CACTGTTGAA GATGCAAACT TTGTGGAAGC CATGGCAGAT
    GCAGGTATTC TCCGTCTGTA CACCTGGGTG GAGTGGGTGA AAGAAATGGT TGCCAACTGG
    GACAGCCTAA GAAGTGGGCC TGCCAGCACC TTCAATGATA GAGTTTTTGC CAGTGAGATG
    AATGCAGGAA TTATAAAAAC AGATCAAAAT TATGAAAAGA TGATGTTTAA AGAAGCCCTG
    AAAACAGGGT TTTTTGAGTT TCAGGCTGCG AAAGATAAAT ACCGTGAATT GGCCATAGAA
    GGTATGCACA GAGACCTTGT GTTCCGCTTT ATTGAAGTTC AAACACTTCT CTTGGCTCCA
    TTCTGTCCAC ACCTATGTGA ACACATCTGG ACACTCCTGG GAAAGCCTGA CTCCATTATG
    AATGCTTCAT GGCCTCTGGC TGGTCCTGTG GATGAAGCCT TGATACGTTC CTCACAGTAT
    CTCATGGAAG TAGCACATGA CCTTAGATTG CGACTCAAGA ACTATATGAT GCCAGCTAAG
    GGGAAGAAGA CTGACAAGCA ACCACCTCAG AAACCTTCAC ATTGCACCAT CTATGTGGCG
    AAGAACTATC CTTCTTGGCA ACATACCACC CTGTCAGTGC TGCGTAATCA CTTTGAGGCC
    AACAGTGGAA AATTGCCTGA TAACAAAGTC ATTGCTAGTG AACTGGGCAA TCTGCCAGAA
    TTGAAGAAAT ATATGAAGAA AGTGATGCCG TTTGTTGCCA TGATTAAGGA AAATCTGGAG
    AAGATGGGAC CTCGTGTTCT GGATTTGCAG CTAGAATTTG ATGAACAGGC GGTGCTGATG
    GAGAATTTAG TTTACCTGAC CAATTCCCTT GAGCTAGAAC ACATAGAAGT CAAGTTTGCC
    TCCGAAGCAG AAGATAAAGT CAGGGAAGAC TGCTGTCCTG GGAAACCACT TAATGTTTTC
    AGGACAGAAC CTGGTGTGTC AGTCTCCCTA GTGAATCCCC AGCCATCAAA TGGCCACTTC
    ACAACCAAAA TTGAAATCAG GCAAGGGGAT AACCGCGATT CTATAATCAG GCGTTTAATG
    AAAATGGACC GAGGCATCAA AGACCTTTCT AAAGTGAAGC TGATGAGATT TGATGATCCA
    CTGTTGGGAC CTCGGCGAGT TCCTGTCCTG GGAAAAGAGC ACACTGGGAA GACTCCCATT
    TCTGAGCATG CTGTTTTCCA TGTGGACCTC ATGAGCAAGA AAATTCATCT CACTGAAAAT
    GGGCTAGGGG CAGATATTGG TGATACAATT ATCTATTTGG TTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.