LARS1 cDNA ORF clone, Homo sapiens(human)

The following LARS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LARS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu03199 NM_020117.11
Latest version!
Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu67988 NM_001317965.1
Latest version!
Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $559.30
$799.00
OHu67988 XM_011537656.3
Latest version!
Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $559.30
$799.00
OHu101697 NM_016460.3
Latest version!
Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu67987 NM_001317964.1
Latest version!
Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu67988 XM_011537656.2 Homo sapiens leucyl-tRNA synthetase (LARS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $559.30
$799.00
OHu03199 NM_020117.10 Homo sapiens leucyl-tRNA synthetase (LARS), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu03199 NM_020117.9 Homo sapiens leucyl-tRNA synthetase (LARS), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu03199
    Clone ID Related Accession (Same CDS sequence) NM_020117.10 , NM_020117.11 , NM_020117.9
    Accession Version NM_020117.10 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-11-02
    Organism Homo sapiens(human)
    Product leucine--tRNA ligase, cytoplasmic isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB091795.1, AB037773.1, AC091887.3 and CK902545.1. This sequence is a reference standard in the RefSeqGene project. On Dec 17, 2015 this sequence version replaced NM_020117.9. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.162211.1, SRR1803614.84918.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
    CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG
    ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT
    TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA
    GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGGCATGT
    GCTGATAAGT TGAAAAGAGA AATAGAGCTG TATGGTTGCC CCCCTGATTT TCCAGATGAA
    GAAGAGGAAG AGGAAGAAAC CAGTGTTAAA ACAGAAGATA TAATAATTAA GGATAAAGCT
    AAAGGAAAAA AGAGTAAAGC TGCTGCTAAA GCTGGATCTT CTAAATACCA GTGGGGCATT
    ATGAAATCCC TTGGCCTGTC TGATGAAGAG ATAGTAAAAT TTTCTGAAGC AGAACATTGG
    CTTGATTATT TCCCGCCACT GGCTATTCAG GATTTAAAAA GAATGGGTTT GAAGGTAGAC
    TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGATGG
    CAATTTTTAA CATTAAGAGA AAGAAACAAA ATTAAATTTG GGAAGCGGTA TACAATTTAC
    TCTCCGAAAG ATGGACAGCC TTGCATGGAT CATGATAGAC AAACTGGAGA GGGTGTTGGA
    CCTCAGGAAT ATACTTTACT CAAATTGAAG GTGCTTGAGC CATACCCATC TAAATTAAGT
    GGCCTGAAAG GTAAAAATAT TTTCTTGGTG GCTGCTACTC TCAGACCTGA GACCATGTTT
    GGGCAGACAA ATTGTTGGGT TCGTCCTGAT ATGAAGTACA TTGGATTTGA GACGGTGAAT
    GGTGATATAT TCATCTGTAC CCAAAAAGCA GCCAGGAATA TGTCATACCA GGGCTTTACC
    AAAGACAATG GCGTGGTGCC TGTTGTTAAG GAATTAATGG GGGAGGAAAT TCTTGGTGCA
    TCACTTTCTG CACCTTTAAC ATCATACAAG GTGATCTATG TTCTCCCAAT GCTAACTATT
    AAGGAGGATA AAGGCACTGG TGTGGTTACA AGTGTTCCTT CCGACTCCCC TGATGATATT
    GCTGCCCTCA GAGACTTGAA GAAAAAGCAA GCCTTACGAG CAAAATATGG AATTAGAGAT
    GACATGGTCT TGCCATTTGA GCCGGTGCCA GTCATTGAAA TCCCAGGTTT TGGAAATCTT
    TCTGCTGTAA CCATTTGTGA TGAGTTGAAA ATTCAGAGCC AGAATGACCG GGAAAAACTT
    GCAGAAGCAA AGGAGAAGAT ATATCTAAAA GGATTTTATG AGGGTATCAT GTTGGTGGAT
    GGATTTAAAG GACAGAAGGT TCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC
    GCTGGAGATG CACTTATTTA CATGGAACCA GAGAAACAAG TGATGTCCAG GTCGTCAGAT
    GAATGTGTTG TGGCTCTGTG TGACCAGTGG TACTTGGATT ATGGAGAAGA GAATTGGAAG
    AAACAGACAT CTCAGTGCTT GAAGAACCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT
    TTTGAAGCCA CCTTAGGTTG GCTACAAGAA CATGCTTGCT CAAGAACTTA TGGTCTAGGC
    ACTCACCTGC CTTGGGATGA GCAGTGGCTG ATTGAATCAC TTTCTGACTC CACTATTTAC
    ATGGCATTTT ACACAGTTGC ACACCTATTG CAGGGGGGTA ACTTGCATGG ACAGGCAGAG
    TCTCCGCTGG GCATTAGACC GCAACAGATG ACCAAGGAAG TTTGGGATTA TGTTTTCTTC
    AAGGAGGCTC CATTTCCTAA GACTCAGATT GCAAAGGAAA AATTAGATCA GTTAAAGCAG
    GAGTTTGAAT TCTGGTATCC TGTTGATCTT CGCGTCTCTG GCAAGGATCT TGTTCCAAAT
    CATCTTTCAT ATTACCTTTA TAATCATGTG GCTATGTGGC CGGAACAAAG TGACAAATGG
    CCTACAGCTG TGAGAGCAAA TGGACATCTC CTCCTGAACT CTGAGAAGAT GTCAAAATCC
    ACAGGCAACT TCCTCACTTT GACCCAAGCT ATTGACAAAT TTTCAGCAGA TGGAATGCGT
    TTGGCTCTGG CTGATGCTGG TGACACTGTA GAAGATGCCA ACTTTGTGGA AGCCATGGCA
    GATGCAGGTA TTCTCCGTCT GTACACCTGG GTAGAGTGGG TGAAAGAAAT GGTTGCCAAC
    TGGGACAGCC TAAGAAGTGG TCCTGCCAGC ACTTTCAATG ATAGAGTTTT TGCCAGTGAA
    TTGAATGCAG GAATTATAAA AACAGATCAA AACTATGAAA AGATGATGTT TAAAGAAGCT
    TTGAAAACAG GGTTTTTTGA GTTTCAGGCC GCAAAAGATA AGTACCGTGA ATTGGCTGTG
    GAAGGGATGC ACAGAGAACT TGTGTTCCGG TTTATTGAAG TTCAGACACT TCTCCTCGCT
    CCATTCTGTC CACATTTGTG TGAGCACATC TGGACACTCC TGGGAAAGCC TGACTCAATT
    ATGAATGCTT CATGGCCTGT GGCAGGTCCT GTTAATGAAG TTTTAATACA CTCCTCACAG
    TATCTTATGG AAGTAACACA TGACCTTAGA CTACGACTCA AGAACTATAT GATGCCAGCT
    AAAGGGAAGA AGACTGACAA ACAACCCCTG CAGAAGCCCT CACATTGCAC CATCTATGTG
    GCAAAGAACT ATCCACCTTG GCAACATACC ACCCTGTCTG TTCTACGTAA ACACTTTGAG
    GCCAATAACG GAAAACTGCC TGACAACAAA GTCATTGCTA GTGAACTAGG CAGTATGCCA
    GAACTGAAGA AATACATGAA GAAAGTCATG CCATTTGTTG CCATGATTAA GGAAAATCTG
    GAGAAGATGG GGCCTCGTAT TCTGGATTTG CAATTAGAAT TTGATGAAAA GGCTGTGCTT
    ATGGAGAATA TAGTCTATCT GACTAATTCG CTTGAGCTAG AACACATAGA AGTCAAGTTT
    GCCTCCGAAG CAGAAGATAA AATCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT
    TTTAGAATAG AACCTGGTGT GTCCGTTTCT CTGGTGAATC CCCAGCCATC CAATGGCCAC
    TTCTCAACCA AAATTGAAAT CAGGCAAGGA GATAACTGTG ATTCCATAAT CAGGCGTTTA
    ATGAAAATGA ATCGAGGAAT TAAAGACCTT TCCAAAGTGA AACTGATGAG ATTTGATGAT
    CCACTGTTGG GGCCTCGACG AGTTCCTGTC CTGGGAAAGG AGTACACCGA GAAGACCCCC
    ATTTCTGAGC ATGCTGTTTT CAATGTGGAC CTCATGAGCA AGAAAATTCA TCTGACTGAG
    AATGGGATAA GGGTGGATAT TGGCGATACA ATAATCTATC TGGTTCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_064502.9
    CDS295..3825
    Translation

    Target ORF information:

    RefSeq Version NM_020117.10
    Organism Homo sapiens(human)
    Definition Homo sapiens leucyl-tRNA synthetase (LARS), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020117.10

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
    CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG
    ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT
    TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA
    GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGGCATGT
    GCTGATAAGT TGAAAAGAGA AATAGAGCTG TATGGTTGCC CCCCTGATTT TCCAGATGAA
    GAAGAGGAAG AGGAAGAAAC CAGTGTTAAA ACAGAAGATA TAATAATTAA GGATAAAGCT
    AAAGGAAAAA AGAGTAAAGC TGCTGCTAAA GCTGGATCTT CTAAATACCA GTGGGGCATT
    ATGAAATCCC TTGGCCTGTC TGATGAAGAG ATAGTAAAAT TTTCTGAAGC AGAACATTGG
    CTTGATTATT TCCCGCCACT GGCTATTCAG GATTTAAAAA GAATGGGTTT GAAGGTAGAC
    TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGATGG
    CAATTTTTAA CATTAAGAGA AAGAAACAAA ATTAAATTTG GGAAGCGGTA TACAATTTAC
    TCTCCGAAAG ATGGACAGCC TTGCATGGAT CATGATAGAC AAACTGGAGA GGGTGTTGGA
    CCTCAGGAAT ATACTTTACT CAAATTGAAG GTGCTTGAGC CATACCCATC TAAATTAAGT
    GGCCTGAAAG GTAAAAATAT TTTCTTGGTG GCTGCTACTC TCAGACCTGA GACCATGTTT
    GGGCAGACAA ATTGTTGGGT TCGTCCTGAT ATGAAGTACA TTGGATTTGA GACGGTGAAT
    GGTGATATAT TCATCTGTAC CCAAAAAGCA GCCAGGAATA TGTCATACCA GGGCTTTACC
    AAAGACAATG GCGTGGTGCC TGTTGTTAAG GAATTAATGG GGGAGGAAAT TCTTGGTGCA
    TCACTTTCTG CACCTTTAAC ATCATACAAG GTGATCTATG TTCTCCCAAT GCTAACTATT
    AAGGAGGATA AAGGCACTGG TGTGGTTACA AGTGTTCCTT CCGACTCCCC TGATGATATT
    GCTGCCCTCA GAGACTTGAA GAAAAAGCAA GCCTTACGAG CAAAATATGG AATTAGAGAT
    GACATGGTCT TGCCATTTGA GCCGGTGCCA GTCATTGAAA TCCCAGGTTT TGGAAATCTT
    TCTGCTGTAA CCATTTGTGA TGAGTTGAAA ATTCAGAGCC AGAATGACCG GGAAAAACTT
    GCAGAAGCAA AGGAGAAGAT ATATCTAAAA GGATTTTATG AGGGTATCAT GTTGGTGGAT
    GGATTTAAAG GACAGAAGGT TCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC
    GCTGGAGATG CACTTATTTA CATGGAACCA GAGAAACAAG TGATGTCCAG GTCGTCAGAT
    GAATGTGTTG TGGCTCTGTG TGACCAGTGG TACTTGGATT ATGGAGAAGA GAATTGGAAG
    AAACAGACAT CTCAGTGCTT GAAGAACCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT
    TTTGAAGCCA CCTTAGGTTG GCTACAAGAA CATGCTTGCT CAAGAACTTA TGGTCTAGGC
    ACTCACCTGC CTTGGGATGA GCAGTGGCTG ATTGAATCAC TTTCTGACTC CACTATTTAC
    ATGGCATTTT ACACAGTTGC ACACCTATTG CAGGGGGGTA ACTTGCATGG ACAGGCAGAG
    TCTCCGCTGG GCATTAGACC GCAACAGATG ACCAAGGAAG TTTGGGATTA TGTTTTCTTC
    AAGGAGGCTC CATTTCCTAA GACTCAGATT GCAAAGGAAA AATTAGATCA GTTAAAGCAG
    GAGTTTGAAT TCTGGTATCC TGTTGATCTT CGCGTCTCTG GCAAGGATCT TGTTCCAAAT
    CATCTTTCAT ATTACCTTTA TAATCATGTG GCTATGTGGC CGGAACAAAG TGACAAATGG
    CCTACAGCTG TGAGAGCAAA TGGACATCTC CTCCTGAACT CTGAGAAGAT GTCAAAATCC
    ACAGGCAACT TCCTCACTTT GACCCAAGCT ATTGACAAAT TTTCAGCAGA TGGAATGCGT
    TTGGCTCTGG CTGATGCTGG TGACACTGTA GAAGATGCCA ACTTTGTGGA AGCCATGGCA
    GATGCAGGTA TTCTCCGTCT GTACACCTGG GTAGAGTGGG TGAAAGAAAT GGTTGCCAAC
    TGGGACAGCC TAAGAAGTGG TCCTGCCAGC ACTTTCAATG ATAGAGTTTT TGCCAGTGAA
    TTGAATGCAG GAATTATAAA AACAGATCAA AACTATGAAA AGATGATGTT TAAAGAAGCT
    TTGAAAACAG GGTTTTTTGA GTTTCAGGCC GCAAAAGATA AGTACCGTGA ATTGGCTGTG
    GAAGGGATGC ACAGAGAACT TGTGTTCCGG TTTATTGAAG TTCAGACACT TCTCCTCGCT
    CCATTCTGTC CACATTTGTG TGAGCACATC TGGACACTCC TGGGAAAGCC TGACTCAATT
    ATGAATGCTT CATGGCCTGT GGCAGGTCCT GTTAATGAAG TTTTAATACA CTCCTCACAG
    TATCTTATGG AAGTAACACA TGACCTTAGA CTACGACTCA AGAACTATAT GATGCCAGCT
    AAAGGGAAGA AGACTGACAA ACAACCCCTG CAGAAGCCCT CACATTGCAC CATCTATGTG
    GCAAAGAACT ATCCACCTTG GCAACATACC ACCCTGTCTG TTCTACGTAA ACACTTTGAG
    GCCAATAACG GAAAACTGCC TGACAACAAA GTCATTGCTA GTGAACTAGG CAGTATGCCA
    GAACTGAAGA AATACATGAA GAAAGTCATG CCATTTGTTG CCATGATTAA GGAAAATCTG
    GAGAAGATGG GGCCTCGTAT TCTGGATTTG CAATTAGAAT TTGATGAAAA GGCTGTGCTT
    ATGGAGAATA TAGTCTATCT GACTAATTCG CTTGAGCTAG AACACATAGA AGTCAAGTTT
    GCCTCCGAAG CAGAAGATAA AATCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT
    TTTAGAATAG AACCTGGTGT GTCCGTTTCT CTGGTGAATC CCCAGCCATC CAATGGCCAC
    TTCTCAACCA AAATTGAAAT CAGGCAAGGA GATAACTGTG ATTCCATAAT CAGGCGTTTA
    ATGAAAATGA ATCGAGGAAT TAAAGACCTT TCCAAAGTGA AACTGATGAG ATTTGATGAT
    CCACTGTTGG GGCCTCGACG AGTTCCTGTC CTGGGAAAGG AGTACACCGA GAAGACCCCC
    ATTTCTGAGC ATGCTGTTTT CAATGTGGAC CTCATGAGCA AGAAAATTCA TCTGACTGAG
    AATGGGATAA GGGTGGATAT TGGCGATACA ATAATCTATC TGGTTCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu03199
    Clone ID Related Accession (Same CDS sequence) NM_020117.10 , NM_020117.11 , NM_020117.9
    Accession Version NM_020117.11 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-16
    Organism Homo sapiens(human)
    Product leucine--tRNA ligase, cytoplasmic isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB037773.1, AC091887.3 and CK902545.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_020117.10. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.12971.1, SRR1803614.84918.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000394434.7/ ENSP00000377954.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
    CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG
    ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT
    TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA
    GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGGCATGT
    GCTGATAAGT TGAAAAGAGA AATAGAGCTG TATGGTTGCC CCCCTGATTT TCCAGATGAA
    GAAGAGGAAG AGGAAGAAAC CAGTGTTAAA ACAGAAGATA TAATAATTAA GGATAAAGCT
    AAAGGAAAAA AGAGTAAAGC TGCTGCTAAA GCTGGATCTT CTAAATACCA GTGGGGCATT
    ATGAAATCCC TTGGCCTGTC TGATGAAGAG ATAGTAAAAT TTTCTGAAGC AGAACATTGG
    CTTGATTATT TCCCGCCACT GGCTATTCAG GATTTAAAAA GAATGGGTTT GAAGGTAGAC
    TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGATGG
    CAATTTTTAA CATTAAGAGA AAGAAACAAA ATTAAATTTG GGAAGCGGTA TACAATTTAC
    TCTCCGAAAG ATGGACAGCC TTGCATGGAT CATGATAGAC AAACTGGAGA GGGTGTTGGA
    CCTCAGGAAT ATACTTTACT CAAATTGAAG GTGCTTGAGC CATACCCATC TAAATTAAGT
    GGCCTGAAAG GTAAAAATAT TTTCTTGGTG GCTGCTACTC TCAGACCTGA GACCATGTTT
    GGGCAGACAA ATTGTTGGGT TCGTCCTGAT ATGAAGTACA TTGGATTTGA GACGGTGAAT
    GGTGATATAT TCATCTGTAC CCAAAAAGCA GCCAGGAATA TGTCATACCA GGGCTTTACC
    AAAGACAATG GCGTGGTGCC TGTTGTTAAG GAATTAATGG GGGAGGAAAT TCTTGGTGCA
    TCACTTTCTG CACCTTTAAC ATCATACAAG GTGATCTATG TTCTCCCAAT GCTAACTATT
    AAGGAGGATA AAGGCACTGG TGTGGTTACA AGTGTTCCTT CCGACTCCCC TGATGATATT
    GCTGCCCTCA GAGACTTGAA GAAAAAGCAA GCCTTACGAG CAAAATATGG AATTAGAGAT
    GACATGGTCT TGCCATTTGA GCCGGTGCCA GTCATTGAAA TCCCAGGTTT TGGAAATCTT
    TCTGCTGTAA CCATTTGTGA TGAGTTGAAA ATTCAGAGCC AGAATGACCG GGAAAAACTT
    GCAGAAGCAA AGGAGAAGAT ATATCTAAAA GGATTTTATG AGGGTATCAT GTTGGTGGAT
    GGATTTAAAG GACAGAAGGT TCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC
    GCTGGAGATG CACTTATTTA CATGGAACCA GAGAAACAAG TGATGTCCAG GTCGTCAGAT
    GAATGTGTTG TGGCTCTGTG TGACCAGTGG TACTTGGATT ATGGAGAAGA GAATTGGAAG
    AAACAGACAT CTCAGTGCTT GAAGAACCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT
    TTTGAAGCCA CCTTAGGTTG GCTACAAGAA CATGCTTGCT CAAGAACTTA TGGTCTAGGC
    ACTCACCTGC CTTGGGATGA GCAGTGGCTG ATTGAATCAC TTTCTGACTC CACTATTTAC
    ATGGCATTTT ACACAGTTGC ACACCTATTG CAGGGGGGTA ACTTGCATGG ACAGGCAGAG
    TCTCCGCTGG GCATTAGACC GCAACAGATG ACCAAGGAAG TTTGGGATTA TGTTTTCTTC
    AAGGAGGCTC CATTTCCTAA GACTCAGATT GCAAAGGAAA AATTAGATCA GTTAAAGCAG
    GAGTTTGAAT TCTGGTATCC TGTTGATCTT CGCGTCTCTG GCAAGGATCT TGTTCCAAAT
    CATCTTTCAT ATTACCTTTA TAATCATGTG GCTATGTGGC CGGAACAAAG TGACAAATGG
    CCTACAGCTG TGAGAGCAAA TGGACATCTC CTCCTGAACT CTGAGAAGAT GTCAAAATCC
    ACAGGCAACT TCCTCACTTT GACCCAAGCT ATTGACAAAT TTTCAGCAGA TGGAATGCGT
    TTGGCTCTGG CTGATGCTGG TGACACTGTA GAAGATGCCA ACTTTGTGGA AGCCATGGCA
    GATGCAGGTA TTCTCCGTCT GTACACCTGG GTAGAGTGGG TGAAAGAAAT GGTTGCCAAC
    TGGGACAGCC TAAGAAGTGG TCCTGCCAGC ACTTTCAATG ATAGAGTTTT TGCCAGTGAA
    TTGAATGCAG GAATTATAAA AACAGATCAA AACTATGAAA AGATGATGTT TAAAGAAGCT
    TTGAAAACAG GGTTTTTTGA GTTTCAGGCC GCAAAAGATA AGTACCGTGA ATTGGCTGTG
    GAAGGGATGC ACAGAGAACT TGTGTTCCGG TTTATTGAAG TTCAGACACT TCTCCTCGCT
    CCATTCTGTC CACATTTGTG TGAGCACATC TGGACACTCC TGGGAAAGCC TGACTCAATT
    ATGAATGCTT CATGGCCTGT GGCAGGTCCT GTTAATGAAG TTTTAATACA CTCCTCACAG
    TATCTTATGG AAGTAACACA TGACCTTAGA CTACGACTCA AGAACTATAT GATGCCAGCT
    AAAGGGAAGA AGACTGACAA ACAACCCCTG CAGAAGCCCT CACATTGCAC CATCTATGTG
    GCAAAGAACT ATCCACCTTG GCAACATACC ACCCTGTCTG TTCTACGTAA ACACTTTGAG
    GCCAATAACG GAAAACTGCC TGACAACAAA GTCATTGCTA GTGAACTAGG CAGTATGCCA
    GAACTGAAGA AATACATGAA GAAAGTCATG CCATTTGTTG CCATGATTAA GGAAAATCTG
    GAGAAGATGG GGCCTCGTAT TCTGGATTTG CAATTAGAAT TTGATGAAAA GGCTGTGCTT
    ATGGAGAATA TAGTCTATCT GACTAATTCG CTTGAGCTAG AACACATAGA AGTCAAGTTT
    GCCTCCGAAG CAGAAGATAA AATCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT
    TTTAGAATAG AACCTGGTGT GTCCGTTTCT CTGGTGAATC CCCAGCCATC CAATGGCCAC
    TTCTCAACCA AAATTGAAAT CAGGCAAGGA GATAACTGTG ATTCCATAAT CAGGCGTTTA
    ATGAAAATGA ATCGAGGAAT TAAAGACCTT TCCAAAGTGA AACTGATGAG ATTTGATGAT
    CCACTGTTGG GGCCTCGACG AGTTCCTGTC CTGGGAAAGG AGTACACCGA GAAGACCCCC
    ATTTCTGAGC ATGCTGTTTT CAATGTGGAC CTCATGAGCA AGAAAATTCA TCTGACTGAG
    AATGGGATAA GGGTGGATAT TGGCGATACA ATAATCTATC TGGTTCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_064502.9
    CDS158..3688
    Translation

    Target ORF information:

    RefSeq Version NM_020117.11
    Organism Homo sapiens(human)
    Definition Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020117.11

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
    CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG
    ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT
    TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA
    GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGGCATGT
    GCTGATAAGT TGAAAAGAGA AATAGAGCTG TATGGTTGCC CCCCTGATTT TCCAGATGAA
    GAAGAGGAAG AGGAAGAAAC CAGTGTTAAA ACAGAAGATA TAATAATTAA GGATAAAGCT
    AAAGGAAAAA AGAGTAAAGC TGCTGCTAAA GCTGGATCTT CTAAATACCA GTGGGGCATT
    ATGAAATCCC TTGGCCTGTC TGATGAAGAG ATAGTAAAAT TTTCTGAAGC AGAACATTGG
    CTTGATTATT TCCCGCCACT GGCTATTCAG GATTTAAAAA GAATGGGTTT GAAGGTAGAC
    TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGATGG
    CAATTTTTAA CATTAAGAGA AAGAAACAAA ATTAAATTTG GGAAGCGGTA TACAATTTAC
    TCTCCGAAAG ATGGACAGCC TTGCATGGAT CATGATAGAC AAACTGGAGA GGGTGTTGGA
    CCTCAGGAAT ATACTTTACT CAAATTGAAG GTGCTTGAGC CATACCCATC TAAATTAAGT
    GGCCTGAAAG GTAAAAATAT TTTCTTGGTG GCTGCTACTC TCAGACCTGA GACCATGTTT
    GGGCAGACAA ATTGTTGGGT TCGTCCTGAT ATGAAGTACA TTGGATTTGA GACGGTGAAT
    GGTGATATAT TCATCTGTAC CCAAAAAGCA GCCAGGAATA TGTCATACCA GGGCTTTACC
    AAAGACAATG GCGTGGTGCC TGTTGTTAAG GAATTAATGG GGGAGGAAAT TCTTGGTGCA
    TCACTTTCTG CACCTTTAAC ATCATACAAG GTGATCTATG TTCTCCCAAT GCTAACTATT
    AAGGAGGATA AAGGCACTGG TGTGGTTACA AGTGTTCCTT CCGACTCCCC TGATGATATT
    GCTGCCCTCA GAGACTTGAA GAAAAAGCAA GCCTTACGAG CAAAATATGG AATTAGAGAT
    GACATGGTCT TGCCATTTGA GCCGGTGCCA GTCATTGAAA TCCCAGGTTT TGGAAATCTT
    TCTGCTGTAA CCATTTGTGA TGAGTTGAAA ATTCAGAGCC AGAATGACCG GGAAAAACTT
    GCAGAAGCAA AGGAGAAGAT ATATCTAAAA GGATTTTATG AGGGTATCAT GTTGGTGGAT
    GGATTTAAAG GACAGAAGGT TCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC
    GCTGGAGATG CACTTATTTA CATGGAACCA GAGAAACAAG TGATGTCCAG GTCGTCAGAT
    GAATGTGTTG TGGCTCTGTG TGACCAGTGG TACTTGGATT ATGGAGAAGA GAATTGGAAG
    AAACAGACAT CTCAGTGCTT GAAGAACCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT
    TTTGAAGCCA CCTTAGGTTG GCTACAAGAA CATGCTTGCT CAAGAACTTA TGGTCTAGGC
    ACTCACCTGC CTTGGGATGA GCAGTGGCTG ATTGAATCAC TTTCTGACTC CACTATTTAC
    ATGGCATTTT ACACAGTTGC ACACCTATTG CAGGGGGGTA ACTTGCATGG ACAGGCAGAG
    TCTCCGCTGG GCATTAGACC GCAACAGATG ACCAAGGAAG TTTGGGATTA TGTTTTCTTC
    AAGGAGGCTC CATTTCCTAA GACTCAGATT GCAAAGGAAA AATTAGATCA GTTAAAGCAG
    GAGTTTGAAT TCTGGTATCC TGTTGATCTT CGCGTCTCTG GCAAGGATCT TGTTCCAAAT
    CATCTTTCAT ATTACCTTTA TAATCATGTG GCTATGTGGC CGGAACAAAG TGACAAATGG
    CCTACAGCTG TGAGAGCAAA TGGACATCTC CTCCTGAACT CTGAGAAGAT GTCAAAATCC
    ACAGGCAACT TCCTCACTTT GACCCAAGCT ATTGACAAAT TTTCAGCAGA TGGAATGCGT
    TTGGCTCTGG CTGATGCTGG TGACACTGTA GAAGATGCCA ACTTTGTGGA AGCCATGGCA
    GATGCAGGTA TTCTCCGTCT GTACACCTGG GTAGAGTGGG TGAAAGAAAT GGTTGCCAAC
    TGGGACAGCC TAAGAAGTGG TCCTGCCAGC ACTTTCAATG ATAGAGTTTT TGCCAGTGAA
    TTGAATGCAG GAATTATAAA AACAGATCAA AACTATGAAA AGATGATGTT TAAAGAAGCT
    TTGAAAACAG GGTTTTTTGA GTTTCAGGCC GCAAAAGATA AGTACCGTGA ATTGGCTGTG
    GAAGGGATGC ACAGAGAACT TGTGTTCCGG TTTATTGAAG TTCAGACACT TCTCCTCGCT
    CCATTCTGTC CACATTTGTG TGAGCACATC TGGACACTCC TGGGAAAGCC TGACTCAATT
    ATGAATGCTT CATGGCCTGT GGCAGGTCCT GTTAATGAAG TTTTAATACA CTCCTCACAG
    TATCTTATGG AAGTAACACA TGACCTTAGA CTACGACTCA AGAACTATAT GATGCCAGCT
    AAAGGGAAGA AGACTGACAA ACAACCCCTG CAGAAGCCCT CACATTGCAC CATCTATGTG
    GCAAAGAACT ATCCACCTTG GCAACATACC ACCCTGTCTG TTCTACGTAA ACACTTTGAG
    GCCAATAACG GAAAACTGCC TGACAACAAA GTCATTGCTA GTGAACTAGG CAGTATGCCA
    GAACTGAAGA AATACATGAA GAAAGTCATG CCATTTGTTG CCATGATTAA GGAAAATCTG
    GAGAAGATGG GGCCTCGTAT TCTGGATTTG CAATTAGAAT TTGATGAAAA GGCTGTGCTT
    ATGGAGAATA TAGTCTATCT GACTAATTCG CTTGAGCTAG AACACATAGA AGTCAAGTTT
    GCCTCCGAAG CAGAAGATAA AATCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT
    TTTAGAATAG AACCTGGTGT GTCCGTTTCT CTGGTGAATC CCCAGCCATC CAATGGCCAC
    TTCTCAACCA AAATTGAAAT CAGGCAAGGA GATAACTGTG ATTCCATAAT CAGGCGTTTA
    ATGAAAATGA ATCGAGGAAT TAAAGACCTT TCCAAAGTGA AACTGATGAG ATTTGATGAT
    CCACTGTTGG GGCCTCGACG AGTTCCTGTC CTGGGAAAGG AGTACACCGA GAAGACCCCC
    ATTTCTGAGC ATGCTGTTTT CAATGTGGAC CTCATGAGCA AGAAAATTCA TCTGACTGAG
    AATGGGATAA GGGTGGATAT TGGCGATACA ATAATCTATC TGGTTCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu03199
    Clone ID Related Accession (Same CDS sequence) NM_020117.10 , NM_020117.11 , NM_020117.9
    Accession Version NM_020117.9 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-07-29
    Organism Homo sapiens(Human)
    Product leucine--tRNA ligase, cytoplasmic
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
    CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG
    ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT
    TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA
    GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGGCATGT
    GCTGATAAGT TGAAAAGAGA AATAGAGCTG TATGGTTGCC CCCCTGATTT TCCAGATGAA
    GAAGAGGAAG AGGAAGAAAC CAGTGTTAAA ACAGAAGATA TAATAATTAA GGATAAAGCT
    AAAGGAAAAA AGAGTAAAGC TGCTGCTAAA GCTGGATCTT CTAAATACCA GTGGGGCATT
    ATGAAATCCC TTGGCCTGTC TGATGAAGAG ATAGTAAAAT TTTCTGAAGC AGAACATTGG
    CTTGATTATT TCCCGCCACT GGCTATTCAG GATTTAAAAA GAATGGGTTT GAAGGTAGAC
    TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGATGG
    CAATTTTTAA CATTAAGAGA AAGAAACAAA ATTAAATTTG GGAAGCGGTA TACAATTTAC
    TCTCCGAAAG ATGGACAGCC TTGCATGGAT CATGATAGAC AAACTGGAGA GGGTGTTGGA
    CCTCAGGAAT ATACTTTACT CAAATTGAAG GTGCTTGAGC CATACCCATC TAAATTAAGT
    GGCCTGAAAG GTAAAAATAT TTTCTTGGTG GCTGCTACTC TCAGACCTGA GACCATGTTT
    GGGCAGACAA ATTGTTGGGT TCGTCCTGAT ATGAAGTACA TTGGATTTGA GACGGTGAAT
    GGTGATATAT TCATCTGTAC CCAAAAAGCA GCCAGGAATA TGTCATACCA GGGCTTTACC
    AAAGACAATG GCGTGGTGCC TGTTGTTAAG GAATTAATGG GGGAGGAAAT TCTTGGTGCA
    TCACTTTCTG CACCTTTAAC ATCATACAAG GTGATCTATG TTCTCCCAAT GCTAACTATT
    AAGGAGGATA AAGGCACTGG TGTGGTTACA AGTGTTCCTT CCGACTCCCC TGATGATATT
    GCTGCCCTCA GAGACTTGAA GAAAAAGCAA GCCTTACGAG CAAAATATGG AATTAGAGAT
    GACATGGTCT TGCCATTTGA GCCGGTGCCA GTCATTGAAA TCCCAGGTTT TGGAAATCTT
    TCTGCTGTAA CCATTTGTGA TGAGTTGAAA ATTCAGAGCC AGAATGACCG GGAAAAACTT
    GCAGAAGCAA AGGAGAAGAT ATATCTAAAA GGATTTTATG AGGGTATCAT GTTGGTGGAT
    GGATTTAAAG GACAGAAGGT TCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC
    GCTGGAGATG CACTTATTTA CATGGAACCA GAGAAACAAG TGATGTCCAG GTCGTCAGAT
    GAATGTGTTG TGGCTCTGTG TGACCAGTGG TACTTGGATT ATGGAGAAGA GAATTGGAAG
    AAACAGACAT CTCAGTGCTT GAAGAACCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT
    TTTGAAGCCA CCTTAGGTTG GCTACAAGAA CATGCTTGCT CAAGAACTTA TGGTCTAGGC
    ACTCACCTGC CTTGGGATGA GCAGTGGCTG ATTGAATCAC TTTCTGACTC CACTATTTAC
    ATGGCATTTT ACACAGTTGC ACACCTATTG CAGGGGGGTA ACTTGCATGG ACAGGCAGAG
    TCTCCGCTGG GCATTAGACC GCAACAGATG ACCAAGGAAG TTTGGGATTA TGTTTTCTTC
    AAGGAGGCTC CATTTCCTAA GACTCAGATT GCAAAGGAAA AATTAGATCA GTTAAAGCAG
    GAGTTTGAAT TCTGGTATCC TGTTGATCTT CGCGTCTCTG GCAAGGATCT TGTTCCAAAT
    CATCTTTCAT ATTACCTTTA TAATCATGTG GCTATGTGGC CGGAACAAAG TGACAAATGG
    CCTACAGCTG TGAGAGCAAA TGGACATCTC CTCCTGAACT CTGAGAAGAT GTCAAAATCC
    ACAGGCAACT TCCTCACTTT GACCCAAGCT ATTGACAAAT TTTCAGCAGA TGGAATGCGT
    TTGGCTCTGG CTGATGCTGG TGACACTGTA GAAGATGCCA ACTTTGTGGA AGCCATGGCA
    GATGCAGGTA TTCTCCGTCT GTACACCTGG GTAGAGTGGG TGAAAGAAAT GGTTGCCAAC
    TGGGACAGCC TAAGAAGTGG TCCTGCCAGC ACTTTCAATG ATAGAGTTTT TGCCAGTGAA
    TTGAATGCAG GAATTATAAA AACAGATCAA AACTATGAAA AGATGATGTT TAAAGAAGCT
    TTGAAAACAG GGTTTTTTGA GTTTCAGGCC GCAAAAGATA AGTACCGTGA ATTGGCTGTG
    GAAGGGATGC ACAGAGAACT TGTGTTCCGG TTTATTGAAG TTCAGACACT TCTCCTCGCT
    CCATTCTGTC CACATTTGTG TGAGCACATC TGGACACTCC TGGGAAAGCC TGACTCAATT
    ATGAATGCTT CATGGCCTGT GGCAGGTCCT GTTAATGAAG TTTTAATACA CTCCTCACAG
    TATCTTATGG AAGTAACACA TGACCTTAGA CTACGACTCA AGAACTATAT GATGCCAGCT
    AAAGGGAAGA AGACTGACAA ACAACCCCTG CAGAAGCCCT CACATTGCAC CATCTATGTG
    GCAAAGAACT ATCCACCTTG GCAACATACC ACCCTGTCTG TTCTACGTAA ACACTTTGAG
    GCCAATAACG GAAAACTGCC TGACAACAAA GTCATTGCTA GTGAACTAGG CAGTATGCCA
    GAACTGAAGA AATACATGAA GAAAGTCATG CCATTTGTTG CCATGATTAA GGAAAATCTG
    GAGAAGATGG GGCCTCGTAT TCTGGATTTG CAATTAGAAT TTGATGAAAA GGCTGTGCTT
    ATGGAGAATA TAGTCTATCT GACTAATTCG CTTGAGCTAG AACACATAGA AGTCAAGTTT
    GCCTCCGAAG CAGAAGATAA AATCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT
    TTTAGAATAG AACCTGGTGT GTCCGTTTCT CTGGTGAATC CCCAGCCATC CAATGGCCAC
    TTCTCAACCA AAATTGAAAT CAGGCAAGGA GATAACTGTG ATTCCATAAT CAGGCGTTTA
    ATGAAAATGA ATCGAGGAAT TAAAGACCTT TCCAAAGTGA AACTGATGAG ATTTGATGAT
    CCACTGTTGG GGCCTCGACG AGTTCCTGTC CTGGGAAAGG AGTACACCGA GAAGACCCCC
    ATTTCTGAGC ATGCTGTTTT CAATGTGGAC CTCATGAGCA AGAAAATTCA TCTGACTGAG
    AATGGGATAA GGGTGGATAT TGGCGATACA ATAATCTATC TGGTTCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_064502.9
    Translation

    Target ORF information:

    RefSeq Version NM_020117.9
    Organism Homo sapiens(Human)
    Definition Homo sapiens leucyl-tRNA synthetase (LARS), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020117.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
    CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG
    ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT
    TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA
    GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGGCATGT
    GCTGATAAGT TGAAAAGAGA AATAGAGCTG TATGGTTGCC CCCCTGATTT TCCAGATGAA
    GAAGAGGAAG AGGAAGAAAC CAGTGTTAAA ACAGAAGATA TAATAATTAA GGATAAAGCT
    AAAGGAAAAA AGAGTAAAGC TGCTGCTAAA GCTGGATCTT CTAAATACCA GTGGGGCATT
    ATGAAATCCC TTGGCCTGTC TGATGAAGAG ATAGTAAAAT TTTCTGAAGC AGAACATTGG
    CTTGATTATT TCCCGCCACT GGCTATTCAG GATTTAAAAA GAATGGGTTT GAAGGTAGAC
    TGGCGTCGTT CCTTCATCAC CACTGATGTT AATCCTTACT ATGATTCATT TGTCAGATGG
    CAATTTTTAA CATTAAGAGA AAGAAACAAA ATTAAATTTG GGAAGCGGTA TACAATTTAC
    TCTCCGAAAG ATGGACAGCC TTGCATGGAT CATGATAGAC AAACTGGAGA GGGTGTTGGA
    CCTCAGGAAT ATACTTTACT CAAATTGAAG GTGCTTGAGC CATACCCATC TAAATTAAGT
    GGCCTGAAAG GTAAAAATAT TTTCTTGGTG GCTGCTACTC TCAGACCTGA GACCATGTTT
    GGGCAGACAA ATTGTTGGGT TCGTCCTGAT ATGAAGTACA TTGGATTTGA GACGGTGAAT
    GGTGATATAT TCATCTGTAC CCAAAAAGCA GCCAGGAATA TGTCATACCA GGGCTTTACC
    AAAGACAATG GCGTGGTGCC TGTTGTTAAG GAATTAATGG GGGAGGAAAT TCTTGGTGCA
    TCACTTTCTG CACCTTTAAC ATCATACAAG GTGATCTATG TTCTCCCAAT GCTAACTATT
    AAGGAGGATA AAGGCACTGG TGTGGTTACA AGTGTTCCTT CCGACTCCCC TGATGATATT
    GCTGCCCTCA GAGACTTGAA GAAAAAGCAA GCCTTACGAG CAAAATATGG AATTAGAGAT
    GACATGGTCT TGCCATTTGA GCCGGTGCCA GTCATTGAAA TCCCAGGTTT TGGAAATCTT
    TCTGCTGTAA CCATTTGTGA TGAGTTGAAA ATTCAGAGCC AGAATGACCG GGAAAAACTT
    GCAGAAGCAA AGGAGAAGAT ATATCTAAAA GGATTTTATG AGGGTATCAT GTTGGTGGAT
    GGATTTAAAG GACAGAAGGT TCAAGATGTA AAGAAGACTA TTCAGAAAAA GATGATTGAC
    GCTGGAGATG CACTTATTTA CATGGAACCA GAGAAACAAG TGATGTCCAG GTCGTCAGAT
    GAATGTGTTG TGGCTCTGTG TGACCAGTGG TACTTGGATT ATGGAGAAGA GAATTGGAAG
    AAACAGACAT CTCAGTGCTT GAAGAACCTG GAAACATTCT GTGAGGAGAC CAGGAGGAAT
    TTTGAAGCCA CCTTAGGTTG GCTACAAGAA CATGCTTGCT CAAGAACTTA TGGTCTAGGC
    ACTCACCTGC CTTGGGATGA GCAGTGGCTG ATTGAATCAC TTTCTGACTC CACTATTTAC
    ATGGCATTTT ACACAGTTGC ACACCTATTG CAGGGGGGTA ACTTGCATGG ACAGGCAGAG
    TCTCCGCTGG GCATTAGACC GCAACAGATG ACCAAGGAAG TTTGGGATTA TGTTTTCTTC
    AAGGAGGCTC CATTTCCTAA GACTCAGATT GCAAAGGAAA AATTAGATCA GTTAAAGCAG
    GAGTTTGAAT TCTGGTATCC TGTTGATCTT CGCGTCTCTG GCAAGGATCT TGTTCCAAAT
    CATCTTTCAT ATTACCTTTA TAATCATGTG GCTATGTGGC CGGAACAAAG TGACAAATGG
    CCTACAGCTG TGAGAGCAAA TGGACATCTC CTCCTGAACT CTGAGAAGAT GTCAAAATCC
    ACAGGCAACT TCCTCACTTT GACCCAAGCT ATTGACAAAT TTTCAGCAGA TGGAATGCGT
    TTGGCTCTGG CTGATGCTGG TGACACTGTA GAAGATGCCA ACTTTGTGGA AGCCATGGCA
    GATGCAGGTA TTCTCCGTCT GTACACCTGG GTAGAGTGGG TGAAAGAAAT GGTTGCCAAC
    TGGGACAGCC TAAGAAGTGG TCCTGCCAGC ACTTTCAATG ATAGAGTTTT TGCCAGTGAA
    TTGAATGCAG GAATTATAAA AACAGATCAA AACTATGAAA AGATGATGTT TAAAGAAGCT
    TTGAAAACAG GGTTTTTTGA GTTTCAGGCC GCAAAAGATA AGTACCGTGA ATTGGCTGTG
    GAAGGGATGC ACAGAGAACT TGTGTTCCGG TTTATTGAAG TTCAGACACT TCTCCTCGCT
    CCATTCTGTC CACATTTGTG TGAGCACATC TGGACACTCC TGGGAAAGCC TGACTCAATT
    ATGAATGCTT CATGGCCTGT GGCAGGTCCT GTTAATGAAG TTTTAATACA CTCCTCACAG
    TATCTTATGG AAGTAACACA TGACCTTAGA CTACGACTCA AGAACTATAT GATGCCAGCT
    AAAGGGAAGA AGACTGACAA ACAACCCCTG CAGAAGCCCT CACATTGCAC CATCTATGTG
    GCAAAGAACT ATCCACCTTG GCAACATACC ACCCTGTCTG TTCTACGTAA ACACTTTGAG
    GCCAATAACG GAAAACTGCC TGACAACAAA GTCATTGCTA GTGAACTAGG CAGTATGCCA
    GAACTGAAGA AATACATGAA GAAAGTCATG CCATTTGTTG CCATGATTAA GGAAAATCTG
    GAGAAGATGG GGCCTCGTAT TCTGGATTTG CAATTAGAAT TTGATGAAAA GGCTGTGCTT
    ATGGAGAATA TAGTCTATCT GACTAATTCG CTTGAGCTAG AACACATAGA AGTCAAGTTT
    GCCTCCGAAG CAGAAGATAA AATCAGGGAA GACTGCTGTC CTGGGAAACC ACTTAATGTT
    TTTAGAATAG AACCTGGTGT GTCCGTTTCT CTGGTGAATC CCCAGCCATC CAATGGCCAC
    TTCTCAACCA AAATTGAAAT CAGGCAAGGA GATAACTGTG ATTCCATAAT CAGGCGTTTA
    ATGAAAATGA ATCGAGGAAT TAAAGACCTT TCCAAAGTGA AACTGATGAG ATTTGATGAT
    CCACTGTTGG GGCCTCGACG AGTTCCTGTC CTGGGAAAGG AGTACACCGA GAAGACCCCC
    ATTTCTGAGC ATGCTGTTTT CAATGTGGAC CTCATGAGCA AGAAAATTCA TCTGACTGAG
    AATGGGATAA GGGTGGATAT TGGCGATACA ATAATCTATC TGGTTCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu67988
    Clone ID Related Accession (Same CDS sequence) NM_001317965.1 , XM_011537656.3 , XM_011537656.2
    Accession Version NM_001317965.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-15
    Organism Homo sapiens(human)
    Product leucine--tRNA ligase, cytoplasmic isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB091795.1, AK295874.1, AB037773.1, AC091887.3 and CK902545.1. Transcript Variant: This variant (4) lacks an alternate exon in the 5' region and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (4) has a shorter N-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295874.1, SRR1803615.42742.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAATGGAC GCCTTCATTT GGGACACACG TTTTCTTTAT CCAAATGTGA GTTTGCTGTA 
    GGGTACCAGC GATTGAAAGG AAAATGTTGT CTGTTTCCCT TTGGCCTGCA CTGTACTGGA
    ATGCCTATTA AGGCATGTGC TGATAAGTTG AAAAGAGAAA TAGAGCTGTA TGGTTGCCCC
    CCTGATTTTC CAGATGAAGA AGAGGAAGAG GAAGAAACCA GTGTTAAAAC AGAAGATATA
    ATAATTAAGG ATAAAGCTAA AGGAAAAAAG AGTAAAGCTG CTGCTAAAGC TGGATCTTCT
    AAATACCAGT GGGGCATTAT GAAATCCCTT GGCCTGTCTG ATGAAGAGAT AGTAAAATTT
    TCTGAAGCAG AACATTGGCT TGATTATTTC CCGCCACTGG CTATTCAGGA TTTAAAAAGA
    ATGGGTTTGA AGGTAGACTG GCGTCGTTCC TTCATCACCA CTGATGTTAA TCCTTACTAT
    GATTCATTTG TCAGATGGCA ATTTTTAACA TTAAGAGAAA GAAACAAAAT TAAATTTGGG
    AAGCGGTATA CAATTTACTC TCCGAAAGAT GGACAGCCTT GCATGGATCA TGATAGACAA
    ACTGGAGAGG GTGTTGGACC TCAGGAATAT ACTTTACTCA AATTGAAGGT GCTTGAGCCA
    TACCCATCTA AATTAAGTGG CCTGAAAGGT AAAAATATTT TCTTGGTGGC TGCTACTCTC
    AGACCTGAGA CCATGTTTGG GCAGACAAAT TGTTGGGTTC GTCCTGATAT GAAGTACATT
    GGATTTGAGA CGGTGAATGG TGATATATTC ATCTGTACCC AAAAAGCAGC CAGGAATATG
    TCATACCAGG GCTTTACCAA AGACAATGGC GTGGTGCCTG TTGTTAAGGA ATTAATGGGG
    GAGGAAATTC TTGGTGCATC ACTTTCTGCA CCTTTAACAT CATACAAGGT GATCTATGTT
    CTCCCAATGC TAACTATTAA GGAGGATAAA GGCACTGGTG TGGTTACAAG TGTTCCTTCC
    GACTCCCCTG ATGATATTGC TGCCCTCAGA GACTTGAAGA AAAAGCAAGC CTTACGAGCA
    AAATATGGAA TTAGAGATGA CATGGTCTTG CCATTTGAGC CGGTGCCAGT CATTGAAATC
    CCAGGTTTTG GAAATCTTTC TGCTGTAACC ATTTGTGATG AGTTGAAAAT TCAGAGCCAG
    AATGACCGGG AAAAACTTGC AGAAGCAAAG GAGAAGATAT ATCTAAAAGG ATTTTATGAG
    GGTATCATGT TGGTGGATGG ATTTAAAGGA CAGAAGGTTC AAGATGTAAA GAAGACTATT
    CAGAAAAAGA TGATTGACGC TGGAGATGCA CTTATTTACA TGGAACCAGA GAAACAAGTG
    ATGTCCAGGT CGTCAGATGA ATGTGTTGTG GCTCTGTGTG ACCAGTGGTA CTTGGATTAT
    GGAGAAGAGA ATTGGAAGAA ACAGACATCT CAGTGCTTGA AGAACCTGGA AACATTCTGT
    GAGGAGACCA GGAGGAATTT TGAAGCCACC TTAGGTTGGC TACAAGAACA TGCTTGCTCA
    AGAACTTATG GTCTAGGCAC TCACCTGCCT TGGGATGAGC AGTGGCTGAT TGAATCACTT
    TCTGACTCCA CTATTTACAT GGCATTTTAC ACAGTTGCAC ACCTATTGCA GGGGGGTAAC
    TTGCATGGAC AGGCAGAGTC TCCGCTGGGC ATTAGACCGC AACAGATGAC CAAGGAAGTT
    TGGGATTATG TTTTCTTCAA GGAGGCTCCA TTTCCTAAGA CTCAGATTGC AAAGGAAAAA
    TTAGATCAGT TAAAGCAGGA GTTTGAATTC TGGTATCCTG TTGATCTTCG CGTCTCTGGC
    AAGGATCTTG TTCCAAATCA TCTTTCATAT TACCTTTATA ATCATGTGGC TATGTGGCCG
    GAACAAAGTG ACAAATGGCC TACAGCTGTG AGAGCAAATG GACATCTCCT CCTGAACTCT
    GAGAAGATGT CAAAATCCAC AGGCAACTTC CTCACTTTGA CCCAAGCTAT TGACAAATTT
    TCAGCAGATG GAATGCGTTT GGCTCTGGCT GATGCTGGTG ACACTGTAGA AGATGCCAAC
    TTTGTGGAAG CCATGGCAGA TGCAGGTATT CTCCGTCTGT ACACCTGGGT AGAGTGGGTG
    AAAGAAATGG TTGCCAACTG GGACAGCCTA AGAAGTGGTC CTGCCAGCAC TTTCAATGAT
    AGAGTTTTTG CCAGTGAATT GAATGCAGGA ATTATAAAAA CAGATCAAAA CTATGAAAAG
    ATGATGTTTA AAGAAGCTTT GAAAACAGGG TTTTTTGAGT TTCAGGCCGC AAAAGATAAG
    TACCGTGAAT TGGCTGTGGA AGGGATGCAC AGAGAACTTG TGTTCCGGTT TATTGAAGTT
    CAGACACTTC TCCTCGCTCC ATTCTGTCCA CATTTGTGTG AGCACATCTG GACACTCCTG
    GGAAAGCCTG ACTCAATTAT GAATGCTTCA TGGCCTGTGG CAGGTCCTGT TAATGAAGTT
    TTAATACACT CCTCACAGTA TCTTATGGAA GTAACACATG ACCTTAGACT ACGACTCAAG
    AACTATATGA TGCCAGCTAA AGGGAAGAAG ACTGACAAAC AACCCCTGCA GAAGCCCTCA
    CATTGCACCA TCTATGTGGC AAAGAACTAT CCACCTTGGC AACATACCAC CCTGTCTGTT
    CTACGTAAAC ACTTTGAGGC CAATAACGGA AAACTGCCTG ACAACAAAGT CATTGCTAGT
    GAACTAGGCA GTATGCCAGA ACTGAAGAAA TACATGAAGA AAGTCATGCC ATTTGTTGCC
    ATGATTAAGG AAAATCTGGA GAAGATGGGG CCTCGTATTC TGGATTTGCA ATTAGAATTT
    GATGAAAAGG CTGTGCTTAT GGAGAATATA GTCTATCTGA CTAATTCGCT TGAGCTAGAA
    CACATAGAAG TCAAGTTTGC CTCCGAAGCA GAAGATAAAA TCAGGGAAGA CTGCTGTCCT
    GGGAAACCAC TTAATGTTTT TAGAATAGAA CCTGGTGTGT CCGTTTCTCT GGTGAATCCC
    CAGCCATCCA ATGGCCACTT CTCAACCAAA ATTGAAATCA GGCAAGGAGA TAACTGTGAT
    TCCATAATCA GGCGTTTAAT GAAAATGAAT CGAGGAATTA AAGACCTTTC CAAAGTGAAA
    CTGATGAGAT TTGATGATCC ACTGTTGGGG CCTCGACGAG TTCCTGTCCT GGGAAAGGAG
    TACACCGAGA AGACCCCCAT TTCTGAGCAT GCTGTTTTCA ATGTGGACCT CATGAGCAAG
    AAAATTCATC TGACTGAGAA TGGGATAAGG GTGGATATTG GCGATACAAT AATCTATCTG
    GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001304894.1
    CDS338..3706
    Translation

    Target ORF information:

    RefSeq Version NM_001317965.1
    Organism Homo sapiens(human)
    Definition Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001317965.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAATGGAC GCCTTCATTT GGGACACACG TTTTCTTTAT CCAAATGTGA GTTTGCTGTA 
    GGGTACCAGC GATTGAAAGG AAAATGTTGT CTGTTTCCCT TTGGCCTGCA CTGTACTGGA
    ATGCCTATTA AGGCATGTGC TGATAAGTTG AAAAGAGAAA TAGAGCTGTA TGGTTGCCCC
    CCTGATTTTC CAGATGAAGA AGAGGAAGAG GAAGAAACCA GTGTTAAAAC AGAAGATATA
    ATAATTAAGG ATAAAGCTAA AGGAAAAAAG AGTAAAGCTG CTGCTAAAGC TGGATCTTCT
    AAATACCAGT GGGGCATTAT GAAATCCCTT GGCCTGTCTG ATGAAGAGAT AGTAAAATTT
    TCTGAAGCAG AACATTGGCT TGATTATTTC CCGCCACTGG CTATTCAGGA TTTAAAAAGA
    ATGGGTTTGA AGGTAGACTG GCGTCGTTCC TTCATCACCA CTGATGTTAA TCCTTACTAT
    GATTCATTTG TCAGATGGCA ATTTTTAACA TTAAGAGAAA GAAACAAAAT TAAATTTGGG
    AAGCGGTATA CAATTTACTC TCCGAAAGAT GGACAGCCTT GCATGGATCA TGATAGACAA
    ACTGGAGAGG GTGTTGGACC TCAGGAATAT ACTTTACTCA AATTGAAGGT GCTTGAGCCA
    TACCCATCTA AATTAAGTGG CCTGAAAGGT AAAAATATTT TCTTGGTGGC TGCTACTCTC
    AGACCTGAGA CCATGTTTGG GCAGACAAAT TGTTGGGTTC GTCCTGATAT GAAGTACATT
    GGATTTGAGA CGGTGAATGG TGATATATTC ATCTGTACCC AAAAAGCAGC CAGGAATATG
    TCATACCAGG GCTTTACCAA AGACAATGGC GTGGTGCCTG TTGTTAAGGA ATTAATGGGG
    GAGGAAATTC TTGGTGCATC ACTTTCTGCA CCTTTAACAT CATACAAGGT GATCTATGTT
    CTCCCAATGC TAACTATTAA GGAGGATAAA GGCACTGGTG TGGTTACAAG TGTTCCTTCC
    GACTCCCCTG ATGATATTGC TGCCCTCAGA GACTTGAAGA AAAAGCAAGC CTTACGAGCA
    AAATATGGAA TTAGAGATGA CATGGTCTTG CCATTTGAGC CGGTGCCAGT CATTGAAATC
    CCAGGTTTTG GAAATCTTTC TGCTGTAACC ATTTGTGATG AGTTGAAAAT TCAGAGCCAG
    AATGACCGGG AAAAACTTGC AGAAGCAAAG GAGAAGATAT ATCTAAAAGG ATTTTATGAG
    GGTATCATGT TGGTGGATGG ATTTAAAGGA CAGAAGGTTC AAGATGTAAA GAAGACTATT
    CAGAAAAAGA TGATTGACGC TGGAGATGCA CTTATTTACA TGGAACCAGA GAAACAAGTG
    ATGTCCAGGT CGTCAGATGA ATGTGTTGTG GCTCTGTGTG ACCAGTGGTA CTTGGATTAT
    GGAGAAGAGA ATTGGAAGAA ACAGACATCT CAGTGCTTGA AGAACCTGGA AACATTCTGT
    GAGGAGACCA GGAGGAATTT TGAAGCCACC TTAGGTTGGC TACAAGAACA TGCTTGCTCA
    AGAACTTATG GTCTAGGCAC TCACCTGCCT TGGGATGAGC AGTGGCTGAT TGAATCACTT
    TCTGACTCCA CTATTTACAT GGCATTTTAC ACAGTTGCAC ACCTATTGCA GGGGGGTAAC
    TTGCATGGAC AGGCAGAGTC TCCGCTGGGC ATTAGACCGC AACAGATGAC CAAGGAAGTT
    TGGGATTATG TTTTCTTCAA GGAGGCTCCA TTTCCTAAGA CTCAGATTGC AAAGGAAAAA
    TTAGATCAGT TAAAGCAGGA GTTTGAATTC TGGTATCCTG TTGATCTTCG CGTCTCTGGC
    AAGGATCTTG TTCCAAATCA TCTTTCATAT TACCTTTATA ATCATGTGGC TATGTGGCCG
    GAACAAAGTG ACAAATGGCC TACAGCTGTG AGAGCAAATG GACATCTCCT CCTGAACTCT
    GAGAAGATGT CAAAATCCAC AGGCAACTTC CTCACTTTGA CCCAAGCTAT TGACAAATTT
    TCAGCAGATG GAATGCGTTT GGCTCTGGCT GATGCTGGTG ACACTGTAGA AGATGCCAAC
    TTTGTGGAAG CCATGGCAGA TGCAGGTATT CTCCGTCTGT ACACCTGGGT AGAGTGGGTG
    AAAGAAATGG TTGCCAACTG GGACAGCCTA AGAAGTGGTC CTGCCAGCAC TTTCAATGAT
    AGAGTTTTTG CCAGTGAATT GAATGCAGGA ATTATAAAAA CAGATCAAAA CTATGAAAAG
    ATGATGTTTA AAGAAGCTTT GAAAACAGGG TTTTTTGAGT TTCAGGCCGC AAAAGATAAG
    TACCGTGAAT TGGCTGTGGA AGGGATGCAC AGAGAACTTG TGTTCCGGTT TATTGAAGTT
    CAGACACTTC TCCTCGCTCC ATTCTGTCCA CATTTGTGTG AGCACATCTG GACACTCCTG
    GGAAAGCCTG ACTCAATTAT GAATGCTTCA TGGCCTGTGG CAGGTCCTGT TAATGAAGTT
    TTAATACACT CCTCACAGTA TCTTATGGAA GTAACACATG ACCTTAGACT ACGACTCAAG
    AACTATATGA TGCCAGCTAA AGGGAAGAAG ACTGACAAAC AACCCCTGCA GAAGCCCTCA
    CATTGCACCA TCTATGTGGC AAAGAACTAT CCACCTTGGC AACATACCAC CCTGTCTGTT
    CTACGTAAAC ACTTTGAGGC CAATAACGGA AAACTGCCTG ACAACAAAGT CATTGCTAGT
    GAACTAGGCA GTATGCCAGA ACTGAAGAAA TACATGAAGA AAGTCATGCC ATTTGTTGCC
    ATGATTAAGG AAAATCTGGA GAAGATGGGG CCTCGTATTC TGGATTTGCA ATTAGAATTT
    GATGAAAAGG CTGTGCTTAT GGAGAATATA GTCTATCTGA CTAATTCGCT TGAGCTAGAA
    CACATAGAAG TCAAGTTTGC CTCCGAAGCA GAAGATAAAA TCAGGGAAGA CTGCTGTCCT
    GGGAAACCAC TTAATGTTTT TAGAATAGAA CCTGGTGTGT CCGTTTCTCT GGTGAATCCC
    CAGCCATCCA ATGGCCACTT CTCAACCAAA ATTGAAATCA GGCAAGGAGA TAACTGTGAT
    TCCATAATCA GGCGTTTAAT GAAAATGAAT CGAGGAATTA AAGACCTTTC CAAAGTGAAA
    CTGATGAGAT TTGATGATCC ACTGTTGGGG CCTCGACGAG TTCCTGTCCT GGGAAAGGAG
    TACACCGAGA AGACCCCCAT TTCTGAGCAT GCTGTTTTCA ATGTGGACCT CATGAGCAAG
    AAAATTCATC TGACTGAGAA TGGGATAAGG GTGGATATTG GCGATACAAT AATCTATCTG
    GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu67988
    Clone ID Related Accession (Same CDS sequence) NM_001317965.1 , XM_011537656.3 , XM_011537656.2
    Accession Version XM_011537656.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product leucine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011537656.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAATGGAC GCCTTCATTT GGGACACACG TTTTCTTTAT CCAAATGTGA GTTTGCTGTA 
    GGGTACCAGC GATTGAAAGG AAAATGTTGT CTGTTTCCCT TTGGCCTGCA CTGTACTGGA
    ATGCCTATTA AGGCATGTGC TGATAAGTTG AAAAGAGAAA TAGAGCTGTA TGGTTGCCCC
    CCTGATTTTC CAGATGAAGA AGAGGAAGAG GAAGAAACCA GTGTTAAAAC AGAAGATATA
    ATAATTAAGG ATAAAGCTAA AGGAAAAAAG AGTAAAGCTG CTGCTAAAGC TGGATCTTCT
    AAATACCAGT GGGGCATTAT GAAATCCCTT GGCCTGTCTG ATGAAGAGAT AGTAAAATTT
    TCTGAAGCAG AACATTGGCT TGATTATTTC CCGCCACTGG CTATTCAGGA TTTAAAAAGA
    ATGGGTTTGA AGGTAGACTG GCGTCGTTCC TTCATCACCA CTGATGTTAA TCCTTACTAT
    GATTCATTTG TCAGATGGCA ATTTTTAACA TTAAGAGAAA GAAACAAAAT TAAATTTGGG
    AAGCGGTATA CAATTTACTC TCCGAAAGAT GGACAGCCTT GCATGGATCA TGATAGACAA
    ACTGGAGAGG GTGTTGGACC TCAGGAATAT ACTTTACTCA AATTGAAGGT GCTTGAGCCA
    TACCCATCTA AATTAAGTGG CCTGAAAGGT AAAAATATTT TCTTGGTGGC TGCTACTCTC
    AGACCTGAGA CCATGTTTGG GCAGACAAAT TGTTGGGTTC GTCCTGATAT GAAGTACATT
    GGATTTGAGA CGGTGAATGG TGATATATTC ATCTGTACCC AAAAAGCAGC CAGGAATATG
    TCATACCAGG GCTTTACCAA AGACAATGGC GTGGTGCCTG TTGTTAAGGA ATTAATGGGG
    GAGGAAATTC TTGGTGCATC ACTTTCTGCA CCTTTAACAT CATACAAGGT GATCTATGTT
    CTCCCAATGC TAACTATTAA GGAGGATAAA GGCACTGGTG TGGTTACAAG TGTTCCTTCC
    GACTCCCCTG ATGATATTGC TGCCCTCAGA GACTTGAAGA AAAAGCAAGC CTTACGAGCA
    AAATATGGAA TTAGAGATGA CATGGTCTTG CCATTTGAGC CGGTGCCAGT CATTGAAATC
    CCAGGTTTTG GAAATCTTTC TGCTGTAACC ATTTGTGATG AGTTGAAAAT TCAGAGCCAG
    AATGACCGGG AAAAACTTGC AGAAGCAAAG GAGAAGATAT ATCTAAAAGG ATTTTATGAG
    GGTATCATGT TGGTGGATGG ATTTAAAGGA CAGAAGGTTC AAGATGTAAA GAAGACTATT
    CAGAAAAAGA TGATTGACGC TGGAGATGCA CTTATTTACA TGGAACCAGA GAAACAAGTG
    ATGTCCAGGT CGTCAGATGA ATGTGTTGTG GCTCTGTGTG ACCAGTGGTA CTTGGATTAT
    GGAGAAGAGA ATTGGAAGAA ACAGACATCT CAGTGCTTGA AGAACCTGGA AACATTCTGT
    GAGGAGACCA GGAGGAATTT TGAAGCCACC TTAGGTTGGC TACAAGAACA TGCTTGCTCA
    AGAACTTATG GTCTAGGCAC TCACCTGCCT TGGGATGAGC AGTGGCTGAT TGAATCACTT
    TCTGACTCCA CTATTTACAT GGCATTTTAC ACAGTTGCAC ACCTATTGCA GGGGGGTAAC
    TTGCATGGAC AGGCAGAGTC TCCGCTGGGC ATTAGACCGC AACAGATGAC CAAGGAAGTT
    TGGGATTATG TTTTCTTCAA GGAGGCTCCA TTTCCTAAGA CTCAGATTGC AAAGGAAAAA
    TTAGATCAGT TAAAGCAGGA GTTTGAATTC TGGTATCCTG TTGATCTTCG CGTCTCTGGC
    AAGGATCTTG TTCCAAATCA TCTTTCATAT TACCTTTATA ATCATGTGGC TATGTGGCCG
    GAACAAAGTG ACAAATGGCC TACAGCTGTG AGAGCAAATG GACATCTCCT CCTGAACTCT
    GAGAAGATGT CAAAATCCAC AGGCAACTTC CTCACTTTGA CCCAAGCTAT TGACAAATTT
    TCAGCAGATG GAATGCGTTT GGCTCTGGCT GATGCTGGTG ACACTGTAGA AGATGCCAAC
    TTTGTGGAAG CCATGGCAGA TGCAGGTATT CTCCGTCTGT ACACCTGGGT AGAGTGGGTG
    AAAGAAATGG TTGCCAACTG GGACAGCCTA AGAAGTGGTC CTGCCAGCAC TTTCAATGAT
    AGAGTTTTTG CCAGTGAATT GAATGCAGGA ATTATAAAAA CAGATCAAAA CTATGAAAAG
    ATGATGTTTA AAGAAGCTTT GAAAACAGGG TTTTTTGAGT TTCAGGCCGC AAAAGATAAG
    TACCGTGAAT TGGCTGTGGA AGGGATGCAC AGAGAACTTG TGTTCCGGTT TATTGAAGTT
    CAGACACTTC TCCTCGCTCC ATTCTGTCCA CATTTGTGTG AGCACATCTG GACACTCCTG
    GGAAAGCCTG ACTCAATTAT GAATGCTTCA TGGCCTGTGG CAGGTCCTGT TAATGAAGTT
    TTAATACACT CCTCACAGTA TCTTATGGAA GTAACACATG ACCTTAGACT ACGACTCAAG
    AACTATATGA TGCCAGCTAA AGGGAAGAAG ACTGACAAAC AACCCCTGCA GAAGCCCTCA
    CATTGCACCA TCTATGTGGC AAAGAACTAT CCACCTTGGC AACATACCAC CCTGTCTGTT
    CTACGTAAAC ACTTTGAGGC CAATAACGGA AAACTGCCTG ACAACAAAGT CATTGCTAGT
    GAACTAGGCA GTATGCCAGA ACTGAAGAAA TACATGAAGA AAGTCATGCC ATTTGTTGCC
    ATGATTAAGG AAAATCTGGA GAAGATGGGG CCTCGTATTC TGGATTTGCA ATTAGAATTT
    GATGAAAAGG CTGTGCTTAT GGAGAATATA GTCTATCTGA CTAATTCGCT TGAGCTAGAA
    CACATAGAAG TCAAGTTTGC CTCCGAAGCA GAAGATAAAA TCAGGGAAGA CTGCTGTCCT
    GGGAAACCAC TTAATGTTTT TAGAATAGAA CCTGGTGTGT CCGTTTCTCT GGTGAATCCC
    CAGCCATCCA ATGGCCACTT CTCAACCAAA ATTGAAATCA GGCAAGGAGA TAACTGTGAT
    TCCATAATCA GGCGTTTAAT GAAAATGAAT CGAGGAATTA AAGACCTTTC CAAAGTGAAA
    CTGATGAGAT TTGATGATCC ACTGTTGGGG CCTCGACGAG TTCCTGTCCT GGGAAAGGAG
    TACACCGAGA AGACCCCCAT TTCTGAGCAT GCTGTTTTCA ATGTGGACCT CATGAGCAAG
    AAAATTCATC TGACTGAGAA TGGGATAAGG GTGGATATTG GCGATACAAT AATCTATCTG
    GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011535958.1
    CDS267..3635
    Translation

    Target ORF information:

    RefSeq Version XM_011537656.3
    Organism Homo sapiens(human)
    Definition Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011537656.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAATGGAC GCCTTCATTT GGGACACACG TTTTCTTTAT CCAAATGTGA GTTTGCTGTA 
    GGGTACCAGC GATTGAAAGG AAAATGTTGT CTGTTTCCCT TTGGCCTGCA CTGTACTGGA
    ATGCCTATTA AGGCATGTGC TGATAAGTTG AAAAGAGAAA TAGAGCTGTA TGGTTGCCCC
    CCTGATTTTC CAGATGAAGA AGAGGAAGAG GAAGAAACCA GTGTTAAAAC AGAAGATATA
    ATAATTAAGG ATAAAGCTAA AGGAAAAAAG AGTAAAGCTG CTGCTAAAGC TGGATCTTCT
    AAATACCAGT GGGGCATTAT GAAATCCCTT GGCCTGTCTG ATGAAGAGAT AGTAAAATTT
    TCTGAAGCAG AACATTGGCT TGATTATTTC CCGCCACTGG CTATTCAGGA TTTAAAAAGA
    ATGGGTTTGA AGGTAGACTG GCGTCGTTCC TTCATCACCA CTGATGTTAA TCCTTACTAT
    GATTCATTTG TCAGATGGCA ATTTTTAACA TTAAGAGAAA GAAACAAAAT TAAATTTGGG
    AAGCGGTATA CAATTTACTC TCCGAAAGAT GGACAGCCTT GCATGGATCA TGATAGACAA
    ACTGGAGAGG GTGTTGGACC TCAGGAATAT ACTTTACTCA AATTGAAGGT GCTTGAGCCA
    TACCCATCTA AATTAAGTGG CCTGAAAGGT AAAAATATTT TCTTGGTGGC TGCTACTCTC
    AGACCTGAGA CCATGTTTGG GCAGACAAAT TGTTGGGTTC GTCCTGATAT GAAGTACATT
    GGATTTGAGA CGGTGAATGG TGATATATTC ATCTGTACCC AAAAAGCAGC CAGGAATATG
    TCATACCAGG GCTTTACCAA AGACAATGGC GTGGTGCCTG TTGTTAAGGA ATTAATGGGG
    GAGGAAATTC TTGGTGCATC ACTTTCTGCA CCTTTAACAT CATACAAGGT GATCTATGTT
    CTCCCAATGC TAACTATTAA GGAGGATAAA GGCACTGGTG TGGTTACAAG TGTTCCTTCC
    GACTCCCCTG ATGATATTGC TGCCCTCAGA GACTTGAAGA AAAAGCAAGC CTTACGAGCA
    AAATATGGAA TTAGAGATGA CATGGTCTTG CCATTTGAGC CGGTGCCAGT CATTGAAATC
    CCAGGTTTTG GAAATCTTTC TGCTGTAACC ATTTGTGATG AGTTGAAAAT TCAGAGCCAG
    AATGACCGGG AAAAACTTGC AGAAGCAAAG GAGAAGATAT ATCTAAAAGG ATTTTATGAG
    GGTATCATGT TGGTGGATGG ATTTAAAGGA CAGAAGGTTC AAGATGTAAA GAAGACTATT
    CAGAAAAAGA TGATTGACGC TGGAGATGCA CTTATTTACA TGGAACCAGA GAAACAAGTG
    ATGTCCAGGT CGTCAGATGA ATGTGTTGTG GCTCTGTGTG ACCAGTGGTA CTTGGATTAT
    GGAGAAGAGA ATTGGAAGAA ACAGACATCT CAGTGCTTGA AGAACCTGGA AACATTCTGT
    GAGGAGACCA GGAGGAATTT TGAAGCCACC TTAGGTTGGC TACAAGAACA TGCTTGCTCA
    AGAACTTATG GTCTAGGCAC TCACCTGCCT TGGGATGAGC AGTGGCTGAT TGAATCACTT
    TCTGACTCCA CTATTTACAT GGCATTTTAC ACAGTTGCAC ACCTATTGCA GGGGGGTAAC
    TTGCATGGAC AGGCAGAGTC TCCGCTGGGC ATTAGACCGC AACAGATGAC CAAGGAAGTT
    TGGGATTATG TTTTCTTCAA GGAGGCTCCA TTTCCTAAGA CTCAGATTGC AAAGGAAAAA
    TTAGATCAGT TAAAGCAGGA GTTTGAATTC TGGTATCCTG TTGATCTTCG CGTCTCTGGC
    AAGGATCTTG TTCCAAATCA TCTTTCATAT TACCTTTATA ATCATGTGGC TATGTGGCCG
    GAACAAAGTG ACAAATGGCC TACAGCTGTG AGAGCAAATG GACATCTCCT CCTGAACTCT
    GAGAAGATGT CAAAATCCAC AGGCAACTTC CTCACTTTGA CCCAAGCTAT TGACAAATTT
    TCAGCAGATG GAATGCGTTT GGCTCTGGCT GATGCTGGTG ACACTGTAGA AGATGCCAAC
    TTTGTGGAAG CCATGGCAGA TGCAGGTATT CTCCGTCTGT ACACCTGGGT AGAGTGGGTG
    AAAGAAATGG TTGCCAACTG GGACAGCCTA AGAAGTGGTC CTGCCAGCAC TTTCAATGAT
    AGAGTTTTTG CCAGTGAATT GAATGCAGGA ATTATAAAAA CAGATCAAAA CTATGAAAAG
    ATGATGTTTA AAGAAGCTTT GAAAACAGGG TTTTTTGAGT TTCAGGCCGC AAAAGATAAG
    TACCGTGAAT TGGCTGTGGA AGGGATGCAC AGAGAACTTG TGTTCCGGTT TATTGAAGTT
    CAGACACTTC TCCTCGCTCC ATTCTGTCCA CATTTGTGTG AGCACATCTG GACACTCCTG
    GGAAAGCCTG ACTCAATTAT GAATGCTTCA TGGCCTGTGG CAGGTCCTGT TAATGAAGTT
    TTAATACACT CCTCACAGTA TCTTATGGAA GTAACACATG ACCTTAGACT ACGACTCAAG
    AACTATATGA TGCCAGCTAA AGGGAAGAAG ACTGACAAAC AACCCCTGCA GAAGCCCTCA
    CATTGCACCA TCTATGTGGC AAAGAACTAT CCACCTTGGC AACATACCAC CCTGTCTGTT
    CTACGTAAAC ACTTTGAGGC CAATAACGGA AAACTGCCTG ACAACAAAGT CATTGCTAGT
    GAACTAGGCA GTATGCCAGA ACTGAAGAAA TACATGAAGA AAGTCATGCC ATTTGTTGCC
    ATGATTAAGG AAAATCTGGA GAAGATGGGG CCTCGTATTC TGGATTTGCA ATTAGAATTT
    GATGAAAAGG CTGTGCTTAT GGAGAATATA GTCTATCTGA CTAATTCGCT TGAGCTAGAA
    CACATAGAAG TCAAGTTTGC CTCCGAAGCA GAAGATAAAA TCAGGGAAGA CTGCTGTCCT
    GGGAAACCAC TTAATGTTTT TAGAATAGAA CCTGGTGTGT CCGTTTCTCT GGTGAATCCC
    CAGCCATCCA ATGGCCACTT CTCAACCAAA ATTGAAATCA GGCAAGGAGA TAACTGTGAT
    TCCATAATCA GGCGTTTAAT GAAAATGAAT CGAGGAATTA AAGACCTTTC CAAAGTGAAA
    CTGATGAGAT TTGATGATCC ACTGTTGGGG CCTCGACGAG TTCCTGTCCT GGGAAAGGAG
    TACACCGAGA AGACCCCCAT TTCTGAGCAT GCTGTTTTCA ATGTGGACCT CATGAGCAAG
    AAAATTCATC TGACTGAGAA TGGGATAAGG GTGGATATTG GCGATACAAT AATCTATCTG
    GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu67988
    Clone ID Related Accession (Same CDS sequence) NM_001317965.1 , XM_011537656.3 , XM_011537656.2
    Accession Version XM_011537656.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product leucine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAATGGAC GCCTTCATTT GGGACACACG TTTTCTTTAT CCAAATGTGA GTTTGCTGTA 
    GGGTACCAGC GATTGAAAGG AAAATGTTGT CTGTTTCCCT TTGGCCTGCA CTGTACTGGA
    ATGCCTATTA AGGCATGTGC TGATAAGTTG AAAAGAGAAA TAGAGCTGTA TGGTTGCCCC
    CCTGATTTTC CAGATGAAGA AGAGGAAGAG GAAGAAACCA GTGTTAAAAC AGAAGATATA
    ATAATTAAGG ATAAAGCTAA AGGAAAAAAG AGTAAAGCTG CTGCTAAAGC TGGATCTTCT
    AAATACCAGT GGGGCATTAT GAAATCCCTT GGCCTGTCTG ATGAAGAGAT AGTAAAATTT
    TCTGAAGCAG AACATTGGCT TGATTATTTC CCGCCACTGG CTATTCAGGA TTTAAAAAGA
    ATGGGTTTGA AGGTAGACTG GCGTCGTTCC TTCATCACCA CTGATGTTAA TCCTTACTAT
    GATTCATTTG TCAGATGGCA ATTTTTAACA TTAAGAGAAA GAAACAAAAT TAAATTTGGG
    AAGCGGTATA CAATTTACTC TCCGAAAGAT GGACAGCCTT GCATGGATCA TGATAGACAA
    ACTGGAGAGG GTGTTGGACC TCAGGAATAT ACTTTACTCA AATTGAAGGT GCTTGAGCCA
    TACCCATCTA AATTAAGTGG CCTGAAAGGT AAAAATATTT TCTTGGTGGC TGCTACTCTC
    AGACCTGAGA CCATGTTTGG GCAGACAAAT TGTTGGGTTC GTCCTGATAT GAAGTACATT
    GGATTTGAGA CGGTGAATGG TGATATATTC ATCTGTACCC AAAAAGCAGC CAGGAATATG
    TCATACCAGG GCTTTACCAA AGACAATGGC GTGGTGCCTG TTGTTAAGGA ATTAATGGGG
    GAGGAAATTC TTGGTGCATC ACTTTCTGCA CCTTTAACAT CATACAAGGT GATCTATGTT
    CTCCCAATGC TAACTATTAA GGAGGATAAA GGCACTGGTG TGGTTACAAG TGTTCCTTCC
    GACTCCCCTG ATGATATTGC TGCCCTCAGA GACTTGAAGA AAAAGCAAGC CTTACGAGCA
    AAATATGGAA TTAGAGATGA CATGGTCTTG CCATTTGAGC CGGTGCCAGT CATTGAAATC
    CCAGGTTTTG GAAATCTTTC TGCTGTAACC ATTTGTGATG AGTTGAAAAT TCAGAGCCAG
    AATGACCGGG AAAAACTTGC AGAAGCAAAG GAGAAGATAT ATCTAAAAGG ATTTTATGAG
    GGTATCATGT TGGTGGATGG ATTTAAAGGA CAGAAGGTTC AAGATGTAAA GAAGACTATT
    CAGAAAAAGA TGATTGACGC TGGAGATGCA CTTATTTACA TGGAACCAGA GAAACAAGTG
    ATGTCCAGGT CGTCAGATGA ATGTGTTGTG GCTCTGTGTG ACCAGTGGTA CTTGGATTAT
    GGAGAAGAGA ATTGGAAGAA ACAGACATCT CAGTGCTTGA AGAACCTGGA AACATTCTGT
    GAGGAGACCA GGAGGAATTT TGAAGCCACC TTAGGTTGGC TACAAGAACA TGCTTGCTCA
    AGAACTTATG GTCTAGGCAC TCACCTGCCT TGGGATGAGC AGTGGCTGAT TGAATCACTT
    TCTGACTCCA CTATTTACAT GGCATTTTAC ACAGTTGCAC ACCTATTGCA GGGGGGTAAC
    TTGCATGGAC AGGCAGAGTC TCCGCTGGGC ATTAGACCGC AACAGATGAC CAAGGAAGTT
    TGGGATTATG TTTTCTTCAA GGAGGCTCCA TTTCCTAAGA CTCAGATTGC AAAGGAAAAA
    TTAGATCAGT TAAAGCAGGA GTTTGAATTC TGGTATCCTG TTGATCTTCG CGTCTCTGGC
    AAGGATCTTG TTCCAAATCA TCTTTCATAT TACCTTTATA ATCATGTGGC TATGTGGCCG
    GAACAAAGTG ACAAATGGCC TACAGCTGTG AGAGCAAATG GACATCTCCT CCTGAACTCT
    GAGAAGATGT CAAAATCCAC AGGCAACTTC CTCACTTTGA CCCAAGCTAT TGACAAATTT
    TCAGCAGATG GAATGCGTTT GGCTCTGGCT GATGCTGGTG ACACTGTAGA AGATGCCAAC
    TTTGTGGAAG CCATGGCAGA TGCAGGTATT CTCCGTCTGT ACACCTGGGT AGAGTGGGTG
    AAAGAAATGG TTGCCAACTG GGACAGCCTA AGAAGTGGTC CTGCCAGCAC TTTCAATGAT
    AGAGTTTTTG CCAGTGAATT GAATGCAGGA ATTATAAAAA CAGATCAAAA CTATGAAAAG
    ATGATGTTTA AAGAAGCTTT GAAAACAGGG TTTTTTGAGT TTCAGGCCGC AAAAGATAAG
    TACCGTGAAT TGGCTGTGGA AGGGATGCAC AGAGAACTTG TGTTCCGGTT TATTGAAGTT
    CAGACACTTC TCCTCGCTCC ATTCTGTCCA CATTTGTGTG AGCACATCTG GACACTCCTG
    GGAAAGCCTG ACTCAATTAT GAATGCTTCA TGGCCTGTGG CAGGTCCTGT TAATGAAGTT
    TTAATACACT CCTCACAGTA TCTTATGGAA GTAACACATG ACCTTAGACT ACGACTCAAG
    AACTATATGA TGCCAGCTAA AGGGAAGAAG ACTGACAAAC AACCCCTGCA GAAGCCCTCA
    CATTGCACCA TCTATGTGGC AAAGAACTAT CCACCTTGGC AACATACCAC CCTGTCTGTT
    CTACGTAAAC ACTTTGAGGC CAATAACGGA AAACTGCCTG ACAACAAAGT CATTGCTAGT
    GAACTAGGCA GTATGCCAGA ACTGAAGAAA TACATGAAGA AAGTCATGCC ATTTGTTGCC
    ATGATTAAGG AAAATCTGGA GAAGATGGGG CCTCGTATTC TGGATTTGCA ATTAGAATTT
    GATGAAAAGG CTGTGCTTAT GGAGAATATA GTCTATCTGA CTAATTCGCT TGAGCTAGAA
    CACATAGAAG TCAAGTTTGC CTCCGAAGCA GAAGATAAAA TCAGGGAAGA CTGCTGTCCT
    GGGAAACCAC TTAATGTTTT TAGAATAGAA CCTGGTGTGT CCGTTTCTCT GGTGAATCCC
    CAGCCATCCA ATGGCCACTT CTCAACCAAA ATTGAAATCA GGCAAGGAGA TAACTGTGAT
    TCCATAATCA GGCGTTTAAT GAAAATGAAT CGAGGAATTA AAGACCTTTC CAAAGTGAAA
    CTGATGAGAT TTGATGATCC ACTGTTGGGG CCTCGACGAG TTCCTGTCCT GGGAAAGGAG
    TACACCGAGA AGACCCCCAT TTCTGAGCAT GCTGTTTTCA ATGTGGACCT CATGAGCAAG
    AAAATTCATC TGACTGAGAA TGGGATAAGG GTGGATATTG GCGATACAAT AATCTATCTG
    GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011535958.1
    CDS323..3691
    Translation

    Target ORF information:

    RefSeq Version XM_011537656.2
    Organism Homo sapiens(human)
    Definition Homo sapiens leucyl-tRNA synthetase (LARS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011537656.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAATGGAC GCCTTCATTT GGGACACACG TTTTCTTTAT CCAAATGTGA GTTTGCTGTA 
    GGGTACCAGC GATTGAAAGG AAAATGTTGT CTGTTTCCCT TTGGCCTGCA CTGTACTGGA
    ATGCCTATTA AGGCATGTGC TGATAAGTTG AAAAGAGAAA TAGAGCTGTA TGGTTGCCCC
    CCTGATTTTC CAGATGAAGA AGAGGAAGAG GAAGAAACCA GTGTTAAAAC AGAAGATATA
    ATAATTAAGG ATAAAGCTAA AGGAAAAAAG AGTAAAGCTG CTGCTAAAGC TGGATCTTCT
    AAATACCAGT GGGGCATTAT GAAATCCCTT GGCCTGTCTG ATGAAGAGAT AGTAAAATTT
    TCTGAAGCAG AACATTGGCT TGATTATTTC CCGCCACTGG CTATTCAGGA TTTAAAAAGA
    ATGGGTTTGA AGGTAGACTG GCGTCGTTCC TTCATCACCA CTGATGTTAA TCCTTACTAT
    GATTCATTTG TCAGATGGCA ATTTTTAACA TTAAGAGAAA GAAACAAAAT TAAATTTGGG
    AAGCGGTATA CAATTTACTC TCCGAAAGAT GGACAGCCTT GCATGGATCA TGATAGACAA
    ACTGGAGAGG GTGTTGGACC TCAGGAATAT ACTTTACTCA AATTGAAGGT GCTTGAGCCA
    TACCCATCTA AATTAAGTGG CCTGAAAGGT AAAAATATTT TCTTGGTGGC TGCTACTCTC
    AGACCTGAGA CCATGTTTGG GCAGACAAAT TGTTGGGTTC GTCCTGATAT GAAGTACATT
    GGATTTGAGA CGGTGAATGG TGATATATTC ATCTGTACCC AAAAAGCAGC CAGGAATATG
    TCATACCAGG GCTTTACCAA AGACAATGGC GTGGTGCCTG TTGTTAAGGA ATTAATGGGG
    GAGGAAATTC TTGGTGCATC ACTTTCTGCA CCTTTAACAT CATACAAGGT GATCTATGTT
    CTCCCAATGC TAACTATTAA GGAGGATAAA GGCACTGGTG TGGTTACAAG TGTTCCTTCC
    GACTCCCCTG ATGATATTGC TGCCCTCAGA GACTTGAAGA AAAAGCAAGC CTTACGAGCA
    AAATATGGAA TTAGAGATGA CATGGTCTTG CCATTTGAGC CGGTGCCAGT CATTGAAATC
    CCAGGTTTTG GAAATCTTTC TGCTGTAACC ATTTGTGATG AGTTGAAAAT TCAGAGCCAG
    AATGACCGGG AAAAACTTGC AGAAGCAAAG GAGAAGATAT ATCTAAAAGG ATTTTATGAG
    GGTATCATGT TGGTGGATGG ATTTAAAGGA CAGAAGGTTC AAGATGTAAA GAAGACTATT
    CAGAAAAAGA TGATTGACGC TGGAGATGCA CTTATTTACA TGGAACCAGA GAAACAAGTG
    ATGTCCAGGT CGTCAGATGA ATGTGTTGTG GCTCTGTGTG ACCAGTGGTA CTTGGATTAT
    GGAGAAGAGA ATTGGAAGAA ACAGACATCT CAGTGCTTGA AGAACCTGGA AACATTCTGT
    GAGGAGACCA GGAGGAATTT TGAAGCCACC TTAGGTTGGC TACAAGAACA TGCTTGCTCA
    AGAACTTATG GTCTAGGCAC TCACCTGCCT TGGGATGAGC AGTGGCTGAT TGAATCACTT
    TCTGACTCCA CTATTTACAT GGCATTTTAC ACAGTTGCAC ACCTATTGCA GGGGGGTAAC
    TTGCATGGAC AGGCAGAGTC TCCGCTGGGC ATTAGACCGC AACAGATGAC CAAGGAAGTT
    TGGGATTATG TTTTCTTCAA GGAGGCTCCA TTTCCTAAGA CTCAGATTGC AAAGGAAAAA
    TTAGATCAGT TAAAGCAGGA GTTTGAATTC TGGTATCCTG TTGATCTTCG CGTCTCTGGC
    AAGGATCTTG TTCCAAATCA TCTTTCATAT TACCTTTATA ATCATGTGGC TATGTGGCCG
    GAACAAAGTG ACAAATGGCC TACAGCTGTG AGAGCAAATG GACATCTCCT CCTGAACTCT
    GAGAAGATGT CAAAATCCAC AGGCAACTTC CTCACTTTGA CCCAAGCTAT TGACAAATTT
    TCAGCAGATG GAATGCGTTT GGCTCTGGCT GATGCTGGTG ACACTGTAGA AGATGCCAAC
    TTTGTGGAAG CCATGGCAGA TGCAGGTATT CTCCGTCTGT ACACCTGGGT AGAGTGGGTG
    AAAGAAATGG TTGCCAACTG GGACAGCCTA AGAAGTGGTC CTGCCAGCAC TTTCAATGAT
    AGAGTTTTTG CCAGTGAATT GAATGCAGGA ATTATAAAAA CAGATCAAAA CTATGAAAAG
    ATGATGTTTA AAGAAGCTTT GAAAACAGGG TTTTTTGAGT TTCAGGCCGC AAAAGATAAG
    TACCGTGAAT TGGCTGTGGA AGGGATGCAC AGAGAACTTG TGTTCCGGTT TATTGAAGTT
    CAGACACTTC TCCTCGCTCC ATTCTGTCCA CATTTGTGTG AGCACATCTG GACACTCCTG
    GGAAAGCCTG ACTCAATTAT GAATGCTTCA TGGCCTGTGG CAGGTCCTGT TAATGAAGTT
    TTAATACACT CCTCACAGTA TCTTATGGAA GTAACACATG ACCTTAGACT ACGACTCAAG
    AACTATATGA TGCCAGCTAA AGGGAAGAAG ACTGACAAAC AACCCCTGCA GAAGCCCTCA
    CATTGCACCA TCTATGTGGC AAAGAACTAT CCACCTTGGC AACATACCAC CCTGTCTGTT
    CTACGTAAAC ACTTTGAGGC CAATAACGGA AAACTGCCTG ACAACAAAGT CATTGCTAGT
    GAACTAGGCA GTATGCCAGA ACTGAAGAAA TACATGAAGA AAGTCATGCC ATTTGTTGCC
    ATGATTAAGG AAAATCTGGA GAAGATGGGG CCTCGTATTC TGGATTTGCA ATTAGAATTT
    GATGAAAAGG CTGTGCTTAT GGAGAATATA GTCTATCTGA CTAATTCGCT TGAGCTAGAA
    CACATAGAAG TCAAGTTTGC CTCCGAAGCA GAAGATAAAA TCAGGGAAGA CTGCTGTCCT
    GGGAAACCAC TTAATGTTTT TAGAATAGAA CCTGGTGTGT CCGTTTCTCT GGTGAATCCC
    CAGCCATCCA ATGGCCACTT CTCAACCAAA ATTGAAATCA GGCAAGGAGA TAACTGTGAT
    TCCATAATCA GGCGTTTAAT GAAAATGAAT CGAGGAATTA AAGACCTTTC CAAAGTGAAA
    CTGATGAGAT TTGATGATCC ACTGTTGGGG CCTCGACGAG TTCCTGTCCT GGGAAAGGAG
    TACACCGAGA AGACCCCCAT TTCTGAGCAT GCTGTTTTCA ATGTGGACCT CATGAGCAAG
    AAAATTCATC TGACTGAGAA TGGGATAAGG GTGGATATTG GCGATACAAT AATCTATCTG
    GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101697
    Clone ID Related Accession (Same CDS sequence) NM_016460.3
    Accession Version NM_016460.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-15
    Organism Homo sapiens(human)
    Product leucine--tRNA ligase, cytoplasmic isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB091795.1, AB037773.1, AK293750.1, AC091887.3 and CK902545.1. On Dec 17, 2015 this sequence version replaced NM_016460.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.170620.1, AK293750.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
    CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG
    ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT
    TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGGCATGTG CTGATAAGTT GAAAAGAGAA
    ATAGAGCTGT ATGGTTGCCC CCCTGATTTT CCAGATGAAG AAGAGGAAGA GGAAGAAACC
    AGTGTTAAAA CAGAAGATAT AATAATTAAG GATAAAGCTA AAGGAAAAAA GAGTAAAGCT
    GCTGCTAAAG CTGGATCTTC TAAATACCAG TGGGGCATTA TGAAATCCCT TGGCCTGTCT
    GATGAAGAGA TAGTAAAATT TTCTGAAGCA GAACATTGGC TTGATTATTT CCCGCCACTG
    GCTATTCAGG ATTTAAAAAG AATGGGTTTG AAGGTAGACT GGCGTCGTTC CTTCATCACC
    ACTGATGTTA ATCCTTACTA TGATTCATTT GTCAGATGGC AATTTTTAAC ATTAAGAGAA
    AGAAACAAAA TTAAATTTGG GAAGCGGTAT ACAATTTACT CTCCGAAAGA TGGACAGCCT
    TGCATGGATC ATGATAGACA AACTGGAGAG GGTGTTGGAC CTCAGGAATA TACTTTACTC
    AAATTGAAGG TGCTTGAGCC ATACCCATCT AAATTAAGTG GCCTGAAAGG TAAAAATATT
    TTCTTGGTGG CTGCTACTCT CAGACCTGAG ACCATGTTTG GGCAGACAAA TTGTTGGGTT
    CGTCCTGATA TGAAGTACAT TGGATTTGAG ACGGTGAATG GTGATATATT CATCTGTACC
    CAAAAAGCAG CCAGGAATAT GTCATACCAG GGCTTTACCA AAGACAATGG CGTGGTGCCT
    GTTGTTAAGG AATTAATGGG GGAGGAAATT CTTGGTGCAT CACTTTCTGC ACCTTTAACA
    TCATACAAGG TGATCTATGT TCTCCCAATG CTAACTATTA AGGAGGATAA AGGCACTGGT
    GTGGTTACAA GTGTTCCTTC CGACTCCCCT GATGATATTG CTGCCCTCAG AGACTTGAAG
    AAAAAGCAAG CCTTACGAGC AAAATATGGA ATTAGAGATG ACATGGTCTT GCCATTTGAG
    CCGGTGCCAG TCATTGAAAT CCCAGGTTTT GGAAATCTTT CTGCTGTAAC CATTTGTGAT
    GAGTTGAAAA TTCAGAGCCA GAATGACCGG GAAAAACTTG CAGAAGCAAA GGAGAAGATA
    TATCTAAAAG GATTTTATGA GGGTATCATG TTGGTGGATG GATTTAAAGG ACAGAAGGTT
    CAAGATGTAA AGAAGACTAT TCAGAAAAAG ATGATTGACG CTGGAGATGC ACTTATTTAC
    ATGGAACCAG AGAAACAAGT GATGTCCAGG TCGTCAGATG AATGTGTTGT GGCTCTGTGT
    GACCAGTGGT ACTTGGATTA TGGAGAAGAG AATTGGAAGA AACAGACATC TCAGTGCTTG
    AAGAACCTGG AAACATTCTG TGAGGAGACC AGGAGGAATT TTGAAGCCAC CTTAGGTTGG
    CTACAAGAAC ATGCTTGCTC AAGAACTTAT GGTCTAGGCA CTCACCTGCC TTGGGATGAG
    CAGTGGCTGA TTGAATCACT TTCTGACTCC ACTATTTACA TGGCATTTTA CACAGTTGCA
    CACCTATTGC AGGGGGGTAA CTTGCATGGA CAGGCAGAGT CTCCGCTGGG CATTAGACCG
    CAACAGATGA CCAAGGAAGT TTGGGATTAT GTTTTCTTCA AGGAGGCTCC ATTTCCTAAG
    ACTCAGATTG CAAAGGAAAA ATTAGATCAG TTAAAGCAGG AGTTTGAATT CTGGTATCCT
    GTTGATCTTC GCGTCTCTGG CAAGGATCTT GTTCCAAATC ATCTTTCATA TTACCTTTAT
    AATCATGTGG CTATGTGGCC GGAACAAAGT GACAAATGGC CTACAGCTGT GAGAGCAAAT
    GGACATCTCC TCCTGAACTC TGAGAAGATG TCAAAATCCA CAGGCAACTT CCTCACTTTG
    ACCCAAGCTA TTGACAAATT TTCAGCAGAT GGAATGCGTT TGGCTCTGGC TGATGCTGGT
    GACACTGTAG AAGATGCCAA CTTTGTGGAA GCCATGGCAG ATGCAGGTAT TCTCCGTCTG
    TACACCTGGG TAGAGTGGGT GAAAGAAATG GTTGCCAACT GGGACAGCCT AAGAAGTGGT
    CCTGCCAGCA CTTTCAATGA TAGAGTTTTT GCCAGTGAAT TGAATGCAGG AATTATAAAA
    ACAGATCAAA ACTATGAAAA GATGATGTTT AAAGAAGCTT TGAAAACAGG GTTTTTTGAG
    TTTCAGGCCG CAAAAGATAA GTACCGTGAA TTGGCTGTGG AAGGGATGCA CAGAGAACTT
    GTGTTCCGGT TTATTGAAGT TCAGACACTT CTCCTCGCTC CATTCTGTCC ACATTTGTGT
    GAGCACATCT GGACACTCCT GGGAAAGCCT GACTCAATTA TGAATGCTTC ATGGCCTGTG
    GCAGGTCCTG TTAATGAAGT TTTAATACAC TCCTCACAGT ATCTTATGGA AGTAACACAT
    GACCTTAGAC TACGACTCAA GAACTATATG ATGCCAGCTA AAGGGAAGAA GACTGACAAA
    CAACCCCTGC AGAAGCCCTC ACATTGCACC ATCTATGTGG CAAAGAACTA TCCACCTTGG
    CAACATACCA CCCTGTCTGT TCTACGTAAA CACTTTGAGG CCAATAACGG AAAACTGCCT
    GACAACAAAG TCATTGCTAG TGAACTAGGC AGTATGCCAG AACTGAAGAA ATACATGAAG
    AAAGTCATGC CATTTGTTGC CATGATTAAG GAAAATCTGG AGAAGATGGG GCCTCGTATT
    CTGGATTTGC AATTAGAATT TGATGAAAAG GCTGTGCTTA TGGAGAATAT AGTCTATCTG
    ACTAATTCGC TTGAGCTAGA ACACATAGAA GTCAAGTTTG CCTCCGAAGC AGAAGATAAA
    ATCAGGGAAG ACTGCTGTCC TGGGAAACCA CTTAATGTTT TTAGAATAGA ACCTGGTGTG
    TCCGTTTCTC TGGTGAATCC CCAGCCATCC AATGGCCACT TCTCAACCAA AATTGAAATC
    AGGCAAGGAG ATAACTGTGA TTCCATAATC AGGCGTTTAA TGAAAATGAA TCGAGGAATT
    AAAGACCTTT CCAAAGTGAA ACTGATGAGA TTTGATGATC CACTGTTGGG GCCTCGACGA
    GTTCCTGTCC TGGGAAAGGA GTACACCGAG AAGACCCCCA TTTCTGAGCA TGCTGTTTTC
    AATGTGGACC TCATGAGCAA GAAAATTCAT CTGACTGAGA ATGGGATAAG GGTGGATATT
    GGCGATACAA TAATCTATCT GGTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_057544.2
    CDS295..3744
    Translation

    Target ORF information:

    RefSeq Version NM_016460.3
    Organism Homo sapiens(human)
    Definition Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_016460.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
    CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG
    ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT
    TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGGCATGTG CTGATAAGTT GAAAAGAGAA
    ATAGAGCTGT ATGGTTGCCC CCCTGATTTT CCAGATGAAG AAGAGGAAGA GGAAGAAACC
    AGTGTTAAAA CAGAAGATAT AATAATTAAG GATAAAGCTA AAGGAAAAAA GAGTAAAGCT
    GCTGCTAAAG CTGGATCTTC TAAATACCAG TGGGGCATTA TGAAATCCCT TGGCCTGTCT
    GATGAAGAGA TAGTAAAATT TTCTGAAGCA GAACATTGGC TTGATTATTT CCCGCCACTG
    GCTATTCAGG ATTTAAAAAG AATGGGTTTG AAGGTAGACT GGCGTCGTTC CTTCATCACC
    ACTGATGTTA ATCCTTACTA TGATTCATTT GTCAGATGGC AATTTTTAAC ATTAAGAGAA
    AGAAACAAAA TTAAATTTGG GAAGCGGTAT ACAATTTACT CTCCGAAAGA TGGACAGCCT
    TGCATGGATC ATGATAGACA AACTGGAGAG GGTGTTGGAC CTCAGGAATA TACTTTACTC
    AAATTGAAGG TGCTTGAGCC ATACCCATCT AAATTAAGTG GCCTGAAAGG TAAAAATATT
    TTCTTGGTGG CTGCTACTCT CAGACCTGAG ACCATGTTTG GGCAGACAAA TTGTTGGGTT
    CGTCCTGATA TGAAGTACAT TGGATTTGAG ACGGTGAATG GTGATATATT CATCTGTACC
    CAAAAAGCAG CCAGGAATAT GTCATACCAG GGCTTTACCA AAGACAATGG CGTGGTGCCT
    GTTGTTAAGG AATTAATGGG GGAGGAAATT CTTGGTGCAT CACTTTCTGC ACCTTTAACA
    TCATACAAGG TGATCTATGT TCTCCCAATG CTAACTATTA AGGAGGATAA AGGCACTGGT
    GTGGTTACAA GTGTTCCTTC CGACTCCCCT GATGATATTG CTGCCCTCAG AGACTTGAAG
    AAAAAGCAAG CCTTACGAGC AAAATATGGA ATTAGAGATG ACATGGTCTT GCCATTTGAG
    CCGGTGCCAG TCATTGAAAT CCCAGGTTTT GGAAATCTTT CTGCTGTAAC CATTTGTGAT
    GAGTTGAAAA TTCAGAGCCA GAATGACCGG GAAAAACTTG CAGAAGCAAA GGAGAAGATA
    TATCTAAAAG GATTTTATGA GGGTATCATG TTGGTGGATG GATTTAAAGG ACAGAAGGTT
    CAAGATGTAA AGAAGACTAT TCAGAAAAAG ATGATTGACG CTGGAGATGC ACTTATTTAC
    ATGGAACCAG AGAAACAAGT GATGTCCAGG TCGTCAGATG AATGTGTTGT GGCTCTGTGT
    GACCAGTGGT ACTTGGATTA TGGAGAAGAG AATTGGAAGA AACAGACATC TCAGTGCTTG
    AAGAACCTGG AAACATTCTG TGAGGAGACC AGGAGGAATT TTGAAGCCAC CTTAGGTTGG
    CTACAAGAAC ATGCTTGCTC AAGAACTTAT GGTCTAGGCA CTCACCTGCC TTGGGATGAG
    CAGTGGCTGA TTGAATCACT TTCTGACTCC ACTATTTACA TGGCATTTTA CACAGTTGCA
    CACCTATTGC AGGGGGGTAA CTTGCATGGA CAGGCAGAGT CTCCGCTGGG CATTAGACCG
    CAACAGATGA CCAAGGAAGT TTGGGATTAT GTTTTCTTCA AGGAGGCTCC ATTTCCTAAG
    ACTCAGATTG CAAAGGAAAA ATTAGATCAG TTAAAGCAGG AGTTTGAATT CTGGTATCCT
    GTTGATCTTC GCGTCTCTGG CAAGGATCTT GTTCCAAATC ATCTTTCATA TTACCTTTAT
    AATCATGTGG CTATGTGGCC GGAACAAAGT GACAAATGGC CTACAGCTGT GAGAGCAAAT
    GGACATCTCC TCCTGAACTC TGAGAAGATG TCAAAATCCA CAGGCAACTT CCTCACTTTG
    ACCCAAGCTA TTGACAAATT TTCAGCAGAT GGAATGCGTT TGGCTCTGGC TGATGCTGGT
    GACACTGTAG AAGATGCCAA CTTTGTGGAA GCCATGGCAG ATGCAGGTAT TCTCCGTCTG
    TACACCTGGG TAGAGTGGGT GAAAGAAATG GTTGCCAACT GGGACAGCCT AAGAAGTGGT
    CCTGCCAGCA CTTTCAATGA TAGAGTTTTT GCCAGTGAAT TGAATGCAGG AATTATAAAA
    ACAGATCAAA ACTATGAAAA GATGATGTTT AAAGAAGCTT TGAAAACAGG GTTTTTTGAG
    TTTCAGGCCG CAAAAGATAA GTACCGTGAA TTGGCTGTGG AAGGGATGCA CAGAGAACTT
    GTGTTCCGGT TTATTGAAGT TCAGACACTT CTCCTCGCTC CATTCTGTCC ACATTTGTGT
    GAGCACATCT GGACACTCCT GGGAAAGCCT GACTCAATTA TGAATGCTTC ATGGCCTGTG
    GCAGGTCCTG TTAATGAAGT TTTAATACAC TCCTCACAGT ATCTTATGGA AGTAACACAT
    GACCTTAGAC TACGACTCAA GAACTATATG ATGCCAGCTA AAGGGAAGAA GACTGACAAA
    CAACCCCTGC AGAAGCCCTC ACATTGCACC ATCTATGTGG CAAAGAACTA TCCACCTTGG
    CAACATACCA CCCTGTCTGT TCTACGTAAA CACTTTGAGG CCAATAACGG AAAACTGCCT
    GACAACAAAG TCATTGCTAG TGAACTAGGC AGTATGCCAG AACTGAAGAA ATACATGAAG
    AAAGTCATGC CATTTGTTGC CATGATTAAG GAAAATCTGG AGAAGATGGG GCCTCGTATT
    CTGGATTTGC AATTAGAATT TGATGAAAAG GCTGTGCTTA TGGAGAATAT AGTCTATCTG
    ACTAATTCGC TTGAGCTAGA ACACATAGAA GTCAAGTTTG CCTCCGAAGC AGAAGATAAA
    ATCAGGGAAG ACTGCTGTCC TGGGAAACCA CTTAATGTTT TTAGAATAGA ACCTGGTGTG
    TCCGTTTCTC TGGTGAATCC CCAGCCATCC AATGGCCACT TCTCAACCAA AATTGAAATC
    AGGCAAGGAG ATAACTGTGA TTCCATAATC AGGCGTTTAA TGAAAATGAA TCGAGGAATT
    AAAGACCTTT CCAAAGTGAA ACTGATGAGA TTTGATGATC CACTGTTGGG GCCTCGACGA
    GTTCCTGTCC TGGGAAAGGA GTACACCGAG AAGACCCCCA TTTCTGAGCA TGCTGTTTTC
    AATGTGGACC TCATGAGCAA GAAAATTCAT CTGACTGAGA ATGGGATAAG GGTGGATATT
    GGCGATACAA TAATCTATCT GGTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu67987
    Clone ID Related Accession (Same CDS sequence) NM_001317964.1
    Accession Version NM_001317964.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-15
    Organism Homo sapiens(human)
    Product leucine--tRNA ligase, cytoplasmic isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB091795.1, AB037773.1, AK304134.1, AC091887.3 and CK902545.1. On Dec 17, 2015 this sequence version replaced XM_011537655.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.120976.1, SRR1803613.226077.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
    CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG
    ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT
    TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA
    GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGAGTAAA
    GCTGCTGCTA AAGCTGGATC TTCTAAATAC CAGTGGGGCA TTATGAAATC CCTTGGCCTG
    TCTGATGAAG AGATAGTAAA ATTTTCTGAA GCAGAACATT GGCTTGATTA TTTCCCGCCA
    CTGGCTATTC AGGATTTAAA AAGAATGGGT TTGAAGGTAG ACTGGCGTCG TTCCTTCATC
    ACCACTGATG TTAATCCTTA CTATGATTCA TTTGTCAGAT GGCAATTTTT AACATTAAGA
    GAAAGAAACA AAATTAAATT TGGGAAGCGG TATACAATTT ACTCTCCGAA AGATGGACAG
    CCTTGCATGG ATCATGATAG ACAAACTGGA GAGGGTGTTG GACCTCAGGA ATATACTTTA
    CTCAAATTGA AGGTGCTTGA GCCATACCCA TCTAAATTAA GTGGCCTGAA AGGTAAAAAT
    ATTTTCTTGG TGGCTGCTAC TCTCAGACCT GAGACCATGT TTGGGCAGAC AAATTGTTGG
    GTTCGTCCTG ATATGAAGTA CATTGGATTT GAGACGGTGA ATGGTGATAT ATTCATCTGT
    ACCCAAAAAG CAGCCAGGAA TATGTCATAC CAGGGCTTTA CCAAAGACAA TGGCGTGGTG
    CCTGTTGTTA AGGAATTAAT GGGGGAGGAA ATTCTTGGTG CATCACTTTC TGCACCTTTA
    ACATCATACA AGGTGATCTA TGTTCTCCCA ATGCTAACTA TTAAGGAGGA TAAAGGCACT
    GGTGTGGTTA CAAGTGTTCC TTCCGACTCC CCTGATGATA TTGCTGCCCT CAGAGACTTG
    AAGAAAAAGC AAGCCTTACG AGCAAAATAT GGAATTAGAG ATGACATGGT CTTGCCATTT
    GAGCCGGTGC CAGTCATTGA AATCCCAGGT TTTGGAAATC TTTCTGCTGT AACCATTTGT
    GATGAGTTGA AAATTCAGAG CCAGAATGAC CGGGAAAAAC TTGCAGAAGC AAAGGAGAAG
    ATATATCTAA AAGGATTTTA TGAGGGTATC ATGTTGGTGG ATGGATTTAA AGGACAGAAG
    GTTCAAGATG TAAAGAAGAC TATTCAGAAA AAGATGATTG ACGCTGGAGA TGCACTTATT
    TACATGGAAC CAGAGAAACA AGTGATGTCC AGGTCGTCAG ATGAATGTGT TGTGGCTCTG
    TGTGACCAGT GGTACTTGGA TTATGGAGAA GAGAATTGGA AGAAACAGAC ATCTCAGTGC
    TTGAAGAACC TGGAAACATT CTGTGAGGAG ACCAGGAGGA ATTTTGAAGC CACCTTAGGT
    TGGCTACAAG AACATGCTTG CTCAAGAACT TATGGTCTAG GCACTCACCT GCCTTGGGAT
    GAGCAGTGGC TGATTGAATC ACTTTCTGAC TCCACTATTT ACATGGCATT TTACACAGTT
    GCACACCTAT TGCAGGGGGG TAACTTGCAT GGACAGGCAG AGTCTCCGCT GGGCATTAGA
    CCGCAACAGA TGACCAAGGA AGTTTGGGAT TATGTTTTCT TCAAGGAGGC TCCATTTCCT
    AAGACTCAGA TTGCAAAGGA AAAATTAGAT CAGTTAAAGC AGGAGTTTGA ATTCTGGTAT
    CCTGTTGATC TTCGCGTCTC TGGCAAGGAT CTTGTTCCAA ATCATCTTTC ATATTACCTT
    TATAATCATG TGGCTATGTG GCCGGAACAA AGTGACAAAT GGCCTACAGC TGTGAGAGCA
    AATGGACATC TCCTCCTGAA CTCTGAGAAG ATGTCAAAAT CCACAGGCAA CTTCCTCACT
    TTGACCCAAG CTATTGACAA ATTTTCAGCA GATGGAATGC GTTTGGCTCT GGCTGATGCT
    GGTGACACTG TAGAAGATGC CAACTTTGTG GAAGCCATGG CAGATGCAGG TATTCTCCGT
    CTGTACACCT GGGTAGAGTG GGTGAAAGAA ATGGTTGCCA ACTGGGACAG CCTAAGAAGT
    GGTCCTGCCA GCACTTTCAA TGATAGAGTT TTTGCCAGTG AATTGAATGC AGGAATTATA
    AAAACAGATC AAAACTATGA AAAGATGATG TTTAAAGAAG CTTTGAAAAC AGGGTTTTTT
    GAGTTTCAGG CCGCAAAAGA TAAGTACCGT GAATTGGCTG TGGAAGGGAT GCACAGAGAA
    CTTGTGTTCC GGTTTATTGA AGTTCAGACA CTTCTCCTCG CTCCATTCTG TCCACATTTG
    TGTGAGCACA TCTGGACACT CCTGGGAAAG CCTGACTCAA TTATGAATGC TTCATGGCCT
    GTGGCAGGTC CTGTTAATGA AGTTTTAATA CACTCCTCAC AGTATCTTAT GGAAGTAACA
    CATGACCTTA GACTACGACT CAAGAACTAT ATGATGCCAG CTAAAGGGAA GAAGACTGAC
    AAACAACCCC TGCAGAAGCC CTCACATTGC ACCATCTATG TGGCAAAGAA CTATCCACCT
    TGGCAACATA CCACCCTGTC TGTTCTACGT AAACACTTTG AGGCCAATAA CGGAAAACTG
    CCTGACAACA AAGTCATTGC TAGTGAACTA GGCAGTATGC CAGAACTGAA GAAATACATG
    AAGAAAGTCA TGCCATTTGT TGCCATGATT AAGGAAAATC TGGAGAAGAT GGGGCCTCGT
    ATTCTGGATT TGCAATTAGA ATTTGATGAA AAGGCTGTGC TTATGGAGAA TATAGTCTAT
    CTGACTAATT CGCTTGAGCT AGAACACATA GAAGTCAAGT TTGCCTCCGA AGCAGAAGAT
    AAAATCAGGG AAGACTGCTG TCCTGGGAAA CCACTTAATG TTTTTAGAAT AGAACCTGGT
    GTGTCCGTTT CTCTGGTGAA TCCCCAGCCA TCCAATGGCC ACTTCTCAAC CAAAATTGAA
    ATCAGGCAAG GAGATAACTG TGATTCCATA ATCAGGCGTT TAATGAAAAT GAATCGAGGA
    ATTAAAGACC TTTCCAAAGT GAAACTGATG AGATTTGATG ATCCACTGTT GGGGCCTCGA
    CGAGTTCCTG TCCTGGGAAA GGAGTACACC GAGAAGACCC CCATTTCTGA GCATGCTGTT
    TTCAATGTGG ACCTCATGAG CAAGAAAATT CATCTGACTG AGAATGGGAT AAGGGTGGAT
    ATTGGCGATA CAATAATCTA TCTGGTTCAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001304893.1
    CDS295..3687
    Translation

    Target ORF information:

    RefSeq Version NM_001317964.1
    Organism Homo sapiens(human)
    Definition Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001317964.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCGGAAA GAAAAGGAAC AGCCAAAGTG GACTTTTTGA AGAAGATTGA GAAAGAAATC 
    CAACAGAAAT GGGATACTGA GAGAGTGTTT GAGGTCAATG CATCTAATTT AGAGAAACAG
    ACCAGCAAGG GCAAGTATTT TGTAACCTTC CCATATCCAT ATATGAATGG ACGCCTTCAT
    TTGGGACACA CGTTTTCTTT ATCCAAATGT GAGTTTGCTG TAGGGTACCA GCGATTGAAA
    GGAAAATGTT GTCTGTTTCC CTTTGGCCTG CACTGTACTG GAATGCCTAT TAAGAGTAAA
    GCTGCTGCTA AAGCTGGATC TTCTAAATAC CAGTGGGGCA TTATGAAATC CCTTGGCCTG
    TCTGATGAAG AGATAGTAAA ATTTTCTGAA GCAGAACATT GGCTTGATTA TTTCCCGCCA
    CTGGCTATTC AGGATTTAAA AAGAATGGGT TTGAAGGTAG ACTGGCGTCG TTCCTTCATC
    ACCACTGATG TTAATCCTTA CTATGATTCA TTTGTCAGAT GGCAATTTTT AACATTAAGA
    GAAAGAAACA AAATTAAATT TGGGAAGCGG TATACAATTT ACTCTCCGAA AGATGGACAG
    CCTTGCATGG ATCATGATAG ACAAACTGGA GAGGGTGTTG GACCTCAGGA ATATACTTTA
    CTCAAATTGA AGGTGCTTGA GCCATACCCA TCTAAATTAA GTGGCCTGAA AGGTAAAAAT
    ATTTTCTTGG TGGCTGCTAC TCTCAGACCT GAGACCATGT TTGGGCAGAC AAATTGTTGG
    GTTCGTCCTG ATATGAAGTA CATTGGATTT GAGACGGTGA ATGGTGATAT ATTCATCTGT
    ACCCAAAAAG CAGCCAGGAA TATGTCATAC CAGGGCTTTA CCAAAGACAA TGGCGTGGTG
    CCTGTTGTTA AGGAATTAAT GGGGGAGGAA ATTCTTGGTG CATCACTTTC TGCACCTTTA
    ACATCATACA AGGTGATCTA TGTTCTCCCA ATGCTAACTA TTAAGGAGGA TAAAGGCACT
    GGTGTGGTTA CAAGTGTTCC TTCCGACTCC CCTGATGATA TTGCTGCCCT CAGAGACTTG
    AAGAAAAAGC AAGCCTTACG AGCAAAATAT GGAATTAGAG ATGACATGGT CTTGCCATTT
    GAGCCGGTGC CAGTCATTGA AATCCCAGGT TTTGGAAATC TTTCTGCTGT AACCATTTGT
    GATGAGTTGA AAATTCAGAG CCAGAATGAC CGGGAAAAAC TTGCAGAAGC AAAGGAGAAG
    ATATATCTAA AAGGATTTTA TGAGGGTATC ATGTTGGTGG ATGGATTTAA AGGACAGAAG
    GTTCAAGATG TAAAGAAGAC TATTCAGAAA AAGATGATTG ACGCTGGAGA TGCACTTATT
    TACATGGAAC CAGAGAAACA AGTGATGTCC AGGTCGTCAG ATGAATGTGT TGTGGCTCTG
    TGTGACCAGT GGTACTTGGA TTATGGAGAA GAGAATTGGA AGAAACAGAC ATCTCAGTGC
    TTGAAGAACC TGGAAACATT CTGTGAGGAG ACCAGGAGGA ATTTTGAAGC CACCTTAGGT
    TGGCTACAAG AACATGCTTG CTCAAGAACT TATGGTCTAG GCACTCACCT GCCTTGGGAT
    GAGCAGTGGC TGATTGAATC ACTTTCTGAC TCCACTATTT ACATGGCATT TTACACAGTT
    GCACACCTAT TGCAGGGGGG TAACTTGCAT GGACAGGCAG AGTCTCCGCT GGGCATTAGA
    CCGCAACAGA TGACCAAGGA AGTTTGGGAT TATGTTTTCT TCAAGGAGGC TCCATTTCCT
    AAGACTCAGA TTGCAAAGGA AAAATTAGAT CAGTTAAAGC AGGAGTTTGA ATTCTGGTAT
    CCTGTTGATC TTCGCGTCTC TGGCAAGGAT CTTGTTCCAA ATCATCTTTC ATATTACCTT
    TATAATCATG TGGCTATGTG GCCGGAACAA AGTGACAAAT GGCCTACAGC TGTGAGAGCA
    AATGGACATC TCCTCCTGAA CTCTGAGAAG ATGTCAAAAT CCACAGGCAA CTTCCTCACT
    TTGACCCAAG CTATTGACAA ATTTTCAGCA GATGGAATGC GTTTGGCTCT GGCTGATGCT
    GGTGACACTG TAGAAGATGC CAACTTTGTG GAAGCCATGG CAGATGCAGG TATTCTCCGT
    CTGTACACCT GGGTAGAGTG GGTGAAAGAA ATGGTTGCCA ACTGGGACAG CCTAAGAAGT
    GGTCCTGCCA GCACTTTCAA TGATAGAGTT TTTGCCAGTG AATTGAATGC AGGAATTATA
    AAAACAGATC AAAACTATGA AAAGATGATG TTTAAAGAAG CTTTGAAAAC AGGGTTTTTT
    GAGTTTCAGG CCGCAAAAGA TAAGTACCGT GAATTGGCTG TGGAAGGGAT GCACAGAGAA
    CTTGTGTTCC GGTTTATTGA AGTTCAGACA CTTCTCCTCG CTCCATTCTG TCCACATTTG
    TGTGAGCACA TCTGGACACT CCTGGGAAAG CCTGACTCAA TTATGAATGC TTCATGGCCT
    GTGGCAGGTC CTGTTAATGA AGTTTTAATA CACTCCTCAC AGTATCTTAT GGAAGTAACA
    CATGACCTTA GACTACGACT CAAGAACTAT ATGATGCCAG CTAAAGGGAA GAAGACTGAC
    AAACAACCCC TGCAGAAGCC CTCACATTGC ACCATCTATG TGGCAAAGAA CTATCCACCT
    TGGCAACATA CCACCCTGTC TGTTCTACGT AAACACTTTG AGGCCAATAA CGGAAAACTG
    CCTGACAACA AAGTCATTGC TAGTGAACTA GGCAGTATGC CAGAACTGAA GAAATACATG
    AAGAAAGTCA TGCCATTTGT TGCCATGATT AAGGAAAATC TGGAGAAGAT GGGGCCTCGT
    ATTCTGGATT TGCAATTAGA ATTTGATGAA AAGGCTGTGC TTATGGAGAA TATAGTCTAT
    CTGACTAATT CGCTTGAGCT AGAACACATA GAAGTCAAGT TTGCCTCCGA AGCAGAAGAT
    AAAATCAGGG AAGACTGCTG TCCTGGGAAA CCACTTAATG TTTTTAGAAT AGAACCTGGT
    GTGTCCGTTT CTCTGGTGAA TCCCCAGCCA TCCAATGGCC ACTTCTCAAC CAAAATTGAA
    ATCAGGCAAG GAGATAACTG TGATTCCATA ATCAGGCGTT TAATGAAAAT GAATCGAGGA
    ATTAAAGACC TTTCCAAAGT GAAACTGATG AGATTTGATG ATCCACTGTT GGGGCCTCGA
    CGAGTTCCTG TCCTGGGAAA GGAGTACACC GAGAAGACCC CCATTTCTGA GCATGCTGTT
    TTCAATGTGG ACCTCATGAG CAAGAAAATT CATCTGACTG AGAATGGGAT AAGGGTGGAT
    ATTGGCGATA CAATAATCTA TCTGGTTCAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.