PCM1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCM1
Entrez Gene ID	5108
Full Name	pericentriolar material 1
Synonyms	PTC4,RET/PCM-1
General protein information	Preferred Names pericentriolar material 1 Names pericentriolar material 1 protein PCM-1 hPCM-1 pericentriolar material 1, PCM1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8p22
Summary	The protein encoded by this gene is a component of centriolar satellites, which are electron dense granules scattered around centrosomes. Inhibition studies show that this protein is essential for the correct localization of several centrosomal proteins, and for anchoring microtubules to the centrosome. Chromosomal aberrations involving this gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015].
Disorder MIM:	600299

mRNA and Protein(s)

mRNA	Protein	Name
XM_017013476.1	XP_016868965.1	pericentriolar material 1 protein isoform X11
XM_017013482.1	XP_016868971.1	pericentriolar material 1 protein isoform X16
XM_017013503.1	XP_016868992.1	pericentriolar material 1 protein isoform X30
XM_017013505.1	XP_016868994.1	pericentriolar material 1 protein isoform X31
XM_017013498.1	XP_016868987.1	pericentriolar material 1 protein isoform X28
XM_017013474.1	XP_016868963.1	pericentriolar material 1 protein isoform X10
XM_017013488.1	XP_016868977.1	pericentriolar material 1 protein isoform X21
XM_017013495.1	XP_016868984.1	pericentriolar material 1 protein isoform X26
XM_017013496.1	XP_016868985.1	pericentriolar material 1 protein isoform X26
NM_006197.3	NP_006188.3	pericentriolar material 1 protein isoform 1
NM_001352645.1	NP_001339574.1	pericentriolar material 1 protein isoform 16
NM_001352637.2	NP_001339566.2	pericentriolar material 1 protein isoform 9
NM_001352641.2	NP_001339570.2	pericentriolar material 1 protein isoform 13
XM_011544534.2	XP_011542836.1	pericentriolar material 1 protein isoform X4
XM_024447169.1	XP_024302937.1	pericentriolar material 1 protein isoform X11
XM_017013501.1	XP_016868990.1	pericentriolar material 1 protein isoform X18
NM_001352632.2	NP_001339561.2	pericentriolar material 1 protein isoform 4
NM_001352648.2	NP_001339577.2	pericentriolar material 1 protein isoform 18
NM_001352636.2	NP_001339565.2	pericentriolar material 1 protein isoform 8
NM_001352633.2	NP_001339562.2	pericentriolar material 1 protein isoform 5
NM_001352638.2	NP_001339567.2	pericentriolar material 1 protein isoform 10
NM_001315508.1	NP_001302437.1	pericentriolar material 1 protein isoform 3
NM_001352642.1	NP_001339571.1	pericentriolar material 1 protein isoform 14
NM_001352648.1	NP_001339577.1	pericentriolar material 1 protein isoform 18
NM_001352632.1	NP_001339561.1	pericentriolar material 1 protein isoform 4
NM_001352639.1	NP_001339568.1	pericentriolar material 1 protein isoform 11
NM_001352652.1	NP_001339581.1	pericentriolar material 1 protein isoform 20
XM_017013504.1	XP_016868993.1	pericentriolar material 1 protein isoform X31
XM_017013506.1	XP_016868995.1	pericentriolar material 1 protein isoform X32
XM_017013507.1	XP_016868996.1	pericentriolar material 1 protein isoform X32
XM_017013494.1	XP_016868983.1	pericentriolar material 1 protein isoform X26
XM_017013490.1	XP_016868979.1	pericentriolar material 1 protein isoform X22
XM_011544533.2	XP_011542835.1	pericentriolar material 1 protein isoform X3
XM_011544540.2	XP_011542842.1	pericentriolar material 1 protein isoform X9
XM_017013483.1	XP_016868972.1	pericentriolar material 1 protein isoform X17
NM_001352657.1	NP_001339586.1	pericentriolar material 1 protein isoform 22
NM_001352655.2	NP_001339584.2	pericentriolar material 1 protein isoform 21
NM_001352639.2	NP_001339568.2	pericentriolar material 1 protein isoform 11
NM_001352647.2	NP_001339576.2	pericentriolar material 1 protein isoform 17
NM_001352644.2	NP_001339573.2	pericentriolar material 1 protein isoform 16
XM_011544530.2	XP_011542832.1	pericentriolar material 1 protein isoform X1
XM_024447168.1	XP_024302936.1	pericentriolar material 1 protein isoform X8
XM_017013480.1	XP_016868969.1	pericentriolar material 1 protein isoform X10
XM_017013489.1	XP_016868978.1	pericentriolar material 1 protein isoform X13
XM_017013491.1	XP_016868980.1	pericentriolar material 1 protein isoform X15
XM_017013496.2	XP_016868985.1	pericentriolar material 1 protein isoform X17
NM_001352650.1	NP_001339579.1	pericentriolar material 1 protein isoform 19
XM_017013497.1	XP_016868986.1	pericentriolar material 1 protein isoform X27
XM_017013499.1	XP_016868988.1	pericentriolar material 1 protein isoform X28
XM_017013502.1	XP_016868991.1	pericentriolar material 1 protein isoform X29
XM_017013508.1	XP_016868997.1	pericentriolar material 1 protein isoform X32
XM_017013475.1	XP_016868964.1	pericentriolar material 1 protein isoform X11
XM_017013477.1	XP_016868966.1	pericentriolar material 1 protein isoform X12
NM_001352651.1	NP_001339580.1	pericentriolar material 1 protein isoform 19
NM_001352635.1	NP_001339564.1	pericentriolar material 1 protein isoform 7
NM_001352646.1	NP_001339575.1	pericentriolar material 1 protein isoform 17
NM_001352638.1	NP_001339567.1	pericentriolar material 1 protein isoform 10
NM_001352636.1	NP_001339565.1	pericentriolar material 1 protein isoform 8
NM_001352647.1	NP_001339576.1	pericentriolar material 1 protein isoform 17
NM_001315507.1	NP_001302436.1	pericentriolar material 1 protein isoform 2
NM_001352654.2	NP_001339583.2	pericentriolar material 1 protein isoform 21
NM_001352642.2	NP_001339571.2	pericentriolar material 1 protein isoform 14
NM_001352643.2	NP_001339572.2	pericentriolar material 1 protein isoform 15
NM_001352656.2	NP_001339585.2	pericentriolar material 1 protein isoform 21
NM_001352660.1	NP_001339589.1	pericentriolar material 1 protein isoform 23
NM_001352651.2	NP_001339580.2	pericentriolar material 1 protein isoform 19
NM_001352640.2	NP_001339569.2	pericentriolar material 1 protein isoform 12
NM_001352652.2	NP_001339581.2	pericentriolar material 1 protein isoform 20
NM_001315507.2	NP_001302436.2	pericentriolar material 1 protein isoform 2
XM_011544528.2	XP_011542830.1	pericentriolar material 1 protein isoform X1
XM_011544529.2	XP_011542831.1	pericentriolar material 1 protein isoform X1
XM_024447167.1	XP_024302935.1	pericentriolar material 1 protein isoform X1
XM_011544532.2	XP_011542834.1	pericentriolar material 1 protein isoform X2
XM_006716336.3	XP_006716399.1	pericentriolar material 1 protein isoform X3
XM_017013486.1	XP_016868975.1	pericentriolar material 1 protein isoform X12
XM_017013490.2	XP_016868979.1	pericentriolar material 1 protein isoform X14
XM_011544535.2	XP_011542837.1	pericentriolar material 1 protein isoform X7
XM_017013485.1	XP_016868974.1	pericentriolar material 1 protein isoform X18
XM_017013484.1	XP_016868973.1	pericentriolar material 1 protein isoform X17
XM_017013492.1	XP_016868981.1	pericentriolar material 1 protein isoform X24
XM_017013500.1	XP_016868989.1	pericentriolar material 1 protein isoform X29
XM_017013479.1	XP_016868968.1	pericentriolar material 1 protein isoform X14
NM_001352656.1	NP_001339585.1	pericentriolar material 1 protein isoform 21
NM_001352634.1	NP_001339563.1	pericentriolar material 1 protein isoform 6
NM_001352653.1	NP_001339582.1	pericentriolar material 1 protein isoform 20
NM_001352633.1	NP_001339562.1	pericentriolar material 1 protein isoform 5
NM_001352649.2	NP_001339578.2	pericentriolar material 1 protein isoform 18
NM_001352645.2	NP_001339574.2	pericentriolar material 1 protein isoform 16
NM_001352658.2	NP_001339587.2	pericentriolar material 1 protein isoform 22
NM_001352634.2	NP_001339563.2	pericentriolar material 1 protein isoform 6
NM_001352657.2	NP_001339586.2	pericentriolar material 1 protein isoform 22
NM_001352653.2	NP_001339582.2	pericentriolar material 1 protein isoform 20
NM_001352650.2	NP_001339579.2	pericentriolar material 1 protein isoform 19
NM_001352646.2	NP_001339575.2	pericentriolar material 1 protein isoform 17
XM_017013473.1	XP_016868962.1	pericentriolar material 1 protein isoform X7
XM_017013481.1	XP_016868970.1	pericentriolar material 1 protein isoform X10
NM_001352654.1	NP_001339583.1	pericentriolar material 1 protein isoform 21
NM_001352658.1	NP_001339587.1	pericentriolar material 1 protein isoform 22
NM_001352655.1	NP_001339584.1	pericentriolar material 1 protein isoform 21
NM_001352643.1	NP_001339572.1	pericentriolar material 1 protein isoform 15

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	pcm1	NP_001120252.1
	PCM1	XP_001097950.2
Rattus norvegicus (Norway rat)	Pcm1	NP_112338.1
Pan troglodytes (chimpanzee)	PCM1	XP_001143666.2
Gallus gallus (chicken)	PCM1	NP_989862.1
Homo sapiens (human)	PCM1	NP_006188.3
Canis lupus familiaris (dog)	PCM1	XP_005630005.1
Bos taurus (cattle)	PCM1	XP_005226076.1
Mus musculus (house mouse)	Pcm1	NP_076151.2
Danio rerio (zebrafish)	pcm1	NP_001108595.1

Gene Ontology
Bibliography

Related articles in PubMed

The X-linked deubiquitinase USP9X is an integral component of centrosome.
Wang Q, Tang Y, Xu Y, Xu S, Jiang Y, Dong Q, Zhou Y, Ge W
The Journal of biological chemistry292(31)12874-12884(2017 08)

Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy.
Joachim J, Razi M, Judith D, Wirth M, Calamita E, Encheva V, Dynlacht BD, Snijders AP, O'Reilly N, Jefferies HBJ, Tooze SA
Current biology : CB27(14)2123-2136.e7(2017 Jul)

The centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking.
Conkar D, Culfa E, Odabasi E, Rauniyar N, Yates JR 3rd, Firat-Karalar EN
Journal of cell science130(8)1450-1462(2017 04)

Tethering of an E3 ligase by PCM1 regulates the abundance of centrosomal KIAA0586/Talpid3 and promotes ciliogenesis.
Wang L, Lee K, Malonis R, Sanchez I, Dynlacht BD
eLife5(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that USP9X as an integral component of centrosome where it functions to stabilize PCM1 and CEP55 and to promote centrosome biogenesis; N-terminal domain of USP9X appears to be responsible for physical association of USP9X with PCM1 and CEP55. (USP9X = ubiquitin-specific protease 9X; PCM1 = pericentriolar material 1 protein; CEP55 = 55kDa centrosomal protein)
Title: The X-linked deubiquitinase USP9X is an integral component of centrosome.

In the absence of PCM1, Mib1 destabilizes Talpid3 through poly-ubiquitylation and suppresses cilium assembly.
Title: Tethering of an E3 ligase by PCM1 regulates the abundance of centrosomal KIAA0586/Talpid3 and promotes ciliogenesis.

a novel variant in the PCM1 3'UTR is significantly associated with ovarian cancer
Title: Targeted resequencing of the microRNAome and 3'UTRome reveals functional germline DNA variants with altered prevalence in epithelial ovarian cancer.

Haematological neoplasms associated with t(8;9)(p22;p24); PCM1-JAK2 have features in common and we suggest that they should be recognized as a specific entity in the WHO classification
Title: Should myeloid and lymphoid neoplasms with PCM1-JAK2 and other rearrangements of JAK2 be recognized as specific entities?

no association between the PCM1 gene and schizophrenia in a Japanese population
Title: Four polymorphisms of the pericentriolar material 1 (PCM1) gene are not associated with schizophrenia in a Japanese population.

The following PCM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu103171	NM_001352660.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OHu103163	NM_001352658.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu103163	NM_001352657.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu103174	NM_001352654.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu103174	NM_001352655.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu103174	NM_001352656.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu98037	NM_001352657.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu98037	NM_001352658.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu98038	NM_001352654.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu98038	NM_001352655.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu98038	NM_001352656.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu98037	XM_017013505.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X45, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu98037	XM_017013504.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X44, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu98038	XM_017013508.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X48, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu98038	XM_017013507.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X47, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu98038	XM_017013506.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X46, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu100526	NM_001315508.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu103164	NM_001352639.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu103166	NM_001352643.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu103167	NM_001352648.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu103169	NM_001352645.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu103172	NM_001352646.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu103172	NM_001352647.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu103175	NM_001352642.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu108289	NM_001352642.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98031	NM_001352639.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98032	NM_001352645.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98032	NM_001352644.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98033	NM_001352641.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98034	NM_001352649.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98034	NM_001352648.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98035	NM_001352647.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98035	NM_001352646.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98036	NM_001352643.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu98030	XM_017013491.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98031	XM_017013492.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98032	XM_017013494.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98032	XM_017013495.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98032	XM_017013496.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98033	XM_017013497.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98034	XM_017013498.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98034	XM_017013499.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98035	XM_017013501.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98035	XM_017013500.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98035	XM_017013502.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X42, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98036	XM_017013503.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X43, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu42081	NM_001352640.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu100520	NM_001315507.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu103165	NM_001352650.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu103165	NM_001352651.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu103173	NM_001352633.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu103176	NM_001352634.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu103178	NM_001352638.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu103195	NM_001352632.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu103196	NM_001352635.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu103198	NM_001352636.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu42080	NM_001315507.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98018	NM_001352632.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98019	NM_001352651.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98019	NM_001352650.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98020	NM_001352633.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98023	NM_001352634.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98024	NM_001352652.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98024	NM_001352653.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98027	NM_001352636.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98028	NM_001352637.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98029	NM_001352638.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98018	XM_017013474.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98019	XM_024447168.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98019	XM_017013475.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98019	XM_017013476.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98020	XM_017013477.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98022	XM_017013480.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98022	XM_017013481.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98023	XM_017013482.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98024	XM_017013483.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98024	XM_017013484.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98025	XM_024447169.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98025	XM_017013485.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98026	XM_017013486.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98028	XM_017013489.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98028	XM_017013488.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98029	XM_017013490.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu18014	NM_006197.3 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu18014	NM_001352653.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu18014	NM_001352652.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu42066	XM_006716336.3 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59523	XM_011544530.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59523	XM_024447167.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59523	XM_011544529.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59523	XM_011544528.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59524	XM_011544532.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59525	XM_011544533.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59526	XM_011544534.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59527	XM_011544535.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59532	XM_011544540.2 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59532	XM_017013473.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59533	XM_017013479.1 Latest version!	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu98032	XM_017013496.1	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98029	XM_017013490.1	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCM1 ( NM_001352660.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339589.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 32, mRNA.

Price & Availability↑

CloneID	OHu103171
Clone ID Related Accession (Same CDS sequence)	NM_001352660.1
Accession Version	NM_001352660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 23
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. Transcript Variant: This variant (32), as well as variant 31, encodes isoform 23. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.110692.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339589.1
CDS	760..2352
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQ

Target ORF information:

RefSeq Version	NM_001352660.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352660.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352657.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339586.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 29, mRNA.

Price & Availability↑

CloneID	OHu103163
Clone ID Related Accession (Same CDS sequence)	NM_001352657.1 , NM_001352658.1
Accession Version	NM_001352657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013505.1. Transcript Variant: This variant (29), as well as variant 30, encodes isoform 22. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.6830.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GTATTCATCC AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339586.1
CDS	760..5190
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	NM_001352657.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352657.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GTATTCATCC AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352658.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339587.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 30, mRNA.

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CloneID	OHu103163
Clone ID Related Accession (Same CDS sequence)	NM_001352657.1 , NM_001352658.1
Accession Version	NM_001352658.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013504.1. Transcript Variant: This variant (30), as well as variant 29, encodes isoform 22. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.255713.1, SRR1803611.136011.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GTATTCATCC AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339587.1
CDS	423..4853
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	NM_001352658.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352658.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GTATTCATCC AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352656.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339585.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 28, mRNA.

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CloneID	OHu103174
Clone ID Related Accession (Same CDS sequence)	NM_001352656.1 , NM_001352654.1 , NM_001352655.1
Accession Version	NM_001352656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013507.1. Transcript Variant: This variant (28), as well as variants 26 and 27, encodes isoform 21. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.272039.1, SRR1660803.247232.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339585.1
CDS	760..5025
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	NM_001352656.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352656.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352654.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339583.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 26, mRNA.

Price & Availability↑

CloneID	OHu103174
Clone ID Related Accession (Same CDS sequence)	NM_001352656.1 , NM_001352654.1 , NM_001352655.1
Accession Version	NM_001352654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013506.1. Transcript Variant: This variant (26), as well as variants 27 and 28, encodes isoform 21. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.21183.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339583.1
CDS	423..4688
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	NM_001352654.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352654.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352655.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339584.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 27, mRNA.

Price & Availability↑

CloneID	OHu103174
Clone ID Related Accession (Same CDS sequence)	NM_001352656.1 , NM_001352654.1 , NM_001352655.1
Accession Version	NM_001352655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013508.1. Transcript Variant: This variant (27), as well as variants 26 and 28, encodes isoform 21. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.285496.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339584.1
CDS	576..4841
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	NM_001352655.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352655.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352658.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339587.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 30, mRNA.

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CloneID	OHu98037
Clone ID Related Accession (Same CDS sequence)	XM_017013505.1 , XM_017013504.1 , NM_001352658.2 , NM_001352657.2
Accession Version	NM_001352658.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352658.1. Transcript Variant: This variant (30), as well as variant 29, encodes isoform 22. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.136011.1, SRR1660809.255713.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GTATTCATCC AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339587.2
CDS	292..4722
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	NM_001352658.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352658.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GTATTCATCC AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352657.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339586.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 29, mRNA.

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CloneID	OHu98037
Clone ID Related Accession (Same CDS sequence)	XM_017013505.1 , XM_017013504.1 , NM_001352658.2 , NM_001352657.2
Accession Version	NM_001352657.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352657.1. Transcript Variant: This variant (29), as well as variant 30, encodes isoform 22. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.6830.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GTATTCATCC AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339586.2
CDS	629..5059
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	NM_001352657.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352657.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GTATTCATCC AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352655.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339584.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 27, mRNA.

Price & Availability↑

CloneID	OHu98038
Clone ID Related Accession (Same CDS sequence)	XM_017013506.1 , XM_017013507.1 , NM_001352655.2 , XM_017013508.1 , NM_001352654.2 , NM_001352656.2
Accession Version	NM_001352655.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352655.1. Transcript Variant: This variant (27), as well as variants 26 and 28, encodes isoform 21. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.285496.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339584.2
CDS	445..4710
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	NM_001352655.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352655.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352654.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339583.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 26, mRNA.

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CloneID	OHu98038
Clone ID Related Accession (Same CDS sequence)	XM_017013506.1 , XM_017013507.1 , NM_001352655.2 , XM_017013508.1 , NM_001352654.2 , NM_001352656.2
Accession Version	NM_001352654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352654.1. Transcript Variant: This variant (26), as well as variants 27 and 28, encodes isoform 21. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.21183.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339583.2
CDS	292..4557
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	NM_001352654.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352654.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352656.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339585.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 28, mRNA.

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CloneID	OHu98038
Clone ID Related Accession (Same CDS sequence)	XM_017013506.1 , XM_017013507.1 , NM_001352655.2 , XM_017013508.1 , NM_001352654.2 , NM_001352656.2
Accession Version	NM_001352656.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352656.1. Transcript Variant: This variant (28), as well as variants 26 and 27, encodes isoform 21. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.272039.1, SRR1660803.247232.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339585.2
CDS	629..4894
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	NM_001352656.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352656.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013505.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868994.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X45, mRNA.

Price & Availability↑

CloneID	OHu98037
Clone ID Related Accession (Same CDS sequence)	XM_017013505.1 , XM_017013504.1 , NM_001352658.2 , NM_001352657.2
Accession Version	XM_017013505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X31
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GTATTCATCC AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868994.1
CDS	636..5066
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	XM_017013505.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X45, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013505.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GTATTCATCC AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013504.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868993.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X44, mRNA.

Price & Availability↑

CloneID	OHu98037
Clone ID Related Accession (Same CDS sequence)	XM_017013505.1 , XM_017013504.1 , NM_001352658.2 , NM_001352657.2
Accession Version	XM_017013504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X31
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GTATTCATCC AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868993.1
CDS	517..4947
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	XM_017013504.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X44, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013504.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GTATTCATCC AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013506.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868995.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X46, mRNA.

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CloneID	OHu98038
Clone ID Related Accession (Same CDS sequence)	XM_017013506.1 , XM_017013507.1 , NM_001352655.2 , XM_017013508.1 , NM_001352654.2 , NM_001352656.2
Accession Version	XM_017013506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X32
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868995.1
CDS	496..4761
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	XM_017013506.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X46, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013506.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013507.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868996.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X47, mRNA.

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CloneID	OHu98038
Clone ID Related Accession (Same CDS sequence)	XM_017013506.1 , XM_017013507.1 , NM_001352655.2 , XM_017013508.1 , NM_001352654.2 , NM_001352656.2
Accession Version	XM_017013507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X32
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868996.1
CDS	800..5065
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	XM_017013507.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X47, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013507.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013508.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868997.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X48, mRNA.

Price & Availability↑

CloneID	OHu98038
Clone ID Related Accession (Same CDS sequence)	XM_017013506.1 , XM_017013507.1 , NM_001352655.2 , XM_017013508.1 , NM_001352654.2 , NM_001352656.2
Accession Version	XM_017013508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X32
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868997.1
CDS	440..4705
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	XM_017013508.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X48, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013508.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGTATT CATCCAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001315508.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001302437.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu100526
Clone ID Related Accession (Same CDS sequence)	NM_001315508.1
Accession Version	NM_001315508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BP282954.1, BC133052.1, L27841.1, AC087625.12 and AW237120.1. Transcript Variant: This variant (3) lacks two alternate in-frame segments compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC133052.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGGTACTA CAGTTAATAA TTTAGAAGAA ACTCCCGTTA TTGAAAATCG TAGTTCACAA 
CAACCTGTAA GTGAAGTTTC TACCATCCCA TGTCCTAGAA TTGATACTCA GCAGCTGGAC 
CGGCAAATTA AAGCAATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGAGCA CATGGATGAA 
GTATGCTCCT CGCAGCTTCT AACTTCAGTA AGGCGCATGG TTTTGACCCT TACCCAGCAA 
AATGATGAGA GCAAAGAGTT TGTAAAGTTC TTTCATAAAC AACTTGGAAG TATATTACAG 
GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAAGACT GTGGAGAAGA TCTTCTTGTA 
GAGATATCTG AAGTGTTGTT CAATGAATTG GCTTTCTTTA AGCTTATGCA AGATTTGGAT 
AATAATAGTA TAACTGTTAA ACAGAGATGC AAAAGGAAAA TAGAAGCAAC TGGAGTGATA 
CAATCTTGTG CCAAAGAGGC TAAAAGGATT CTTGAAGATC ATGGCTCACC TGCTGGAGAG 
ATTGATGATG AAGACAAAGA CAAGGATGAA ACTGAAACAG TTAAGCAGAC TCAAACATCT 
GAGGTGTATG ATGGTCCCAA AAATGTAAGA TCTGATATTT CTGATCAAGA GGAAGATGAA 
GAAAGTGAAG GATGTCCAGT GTCTATTAAT TTGTCTAAAG CTGAAACTCA GGCTTTAACT 
AATTATGGAA GTGGAGAAGA TGAAAATGAG GATGAAGAAA TGGAAGAATT TGAAGAAGGC 
CCTGTGGATG TCCAGACTTC CCTCCAGGCT AACACTGAAG CTACTGAAGA AAATGAACAT 
GATGAACAGG AAGCAGAATC TGGTAATATA AGTCAAAAGT CTGATGAAGA AGATTTTGTA 
AAAGTTGAAG ATTTACCACT GAAACTGACA ATATATTCAG AGGCAGATCT AAGAAAGAAA 
ATGGTAGAAG AAGAACAGAA AAACCATTTA TCTGGTGAAA TATGTGAAAT GCAGACCGAA 
GAATTAGCTG GAAATTCTGA GACACTAAAA GAACCTGAAA CGGTGGGAGC CCAGAGTATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001302437.1
CDS	423..6005
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001315508.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001315508.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGGTACTA CAGTTAATAA TTTAGAAGAA ACTCCCGTTA TTGAAAATCG TAGTTCACAA 
CAACCTGTAA GTGAAGTTTC TACCATCCCA TGTCCTAGAA TTGATACTCA GCAGCTGGAC 
CGGCAAATTA AAGCAATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGAGCA CATGGATGAA 
GTATGCTCCT CGCAGCTTCT AACTTCAGTA AGGCGCATGG TTTTGACCCT TACCCAGCAA 
AATGATGAGA GCAAAGAGTT TGTAAAGTTC TTTCATAAAC AACTTGGAAG TATATTACAG 
GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAAGACT GTGGAGAAGA TCTTCTTGTA 
GAGATATCTG AAGTGTTGTT CAATGAATTG GCTTTCTTTA AGCTTATGCA AGATTTGGAT 
AATAATAGTA TAACTGTTAA ACAGAGATGC AAAAGGAAAA TAGAAGCAAC TGGAGTGATA 
CAATCTTGTG CCAAAGAGGC TAAAAGGATT CTTGAAGATC ATGGCTCACC TGCTGGAGAG 
ATTGATGATG AAGACAAAGA CAAGGATGAA ACTGAAACAG TTAAGCAGAC TCAAACATCT 
GAGGTGTATG ATGGTCCCAA AAATGTAAGA TCTGATATTT CTGATCAAGA GGAAGATGAA 
GAAAGTGAAG GATGTCCAGT GTCTATTAAT TTGTCTAAAG CTGAAACTCA GGCTTTAACT 
AATTATGGAA GTGGAGAAGA TGAAAATGAG GATGAAGAAA TGGAAGAATT TGAAGAAGGC 
CCTGTGGATG TCCAGACTTC CCTCCAGGCT AACACTGAAG CTACTGAAGA AAATGAACAT 
GATGAACAGG AAGCAGAATC TGGTAATATA AGTCAAAAGT CTGATGAAGA AGATTTTGTA 
AAAGTTGAAG ATTTACCACT GAAACTGACA ATATATTCAG AGGCAGATCT AAGAAAGAAA 
ATGGTAGAAG AAGAACAGAA AAACCATTTA TCTGGTGAAA TATGTGAAAT GCAGACCGAA 
GAATTAGCTG GAAATTCTGA GACACTAAAA GAACCTGAAA CGGTGGGAGC CCAGAGTATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352639.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339568.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 11, mRNA.

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CloneID	OHu103164
Clone ID Related Accession (Same CDS sequence)	NM_001352639.1
Accession Version	NM_001352639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 11
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013492.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.29208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGATTTTTC TCAGGAGCAC 
ATGGATGAAG TATGCTCCTC GCAGCTTCTA ACTTCAGTAA GGCGCATGGT TTTGACCCTT 
ACCCAGCAAA ATGATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTC AATGAATTGG CTTTCTTTAA GCTTATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCAACT 
GGAGTGATAC AATCTTGTGC CAAAGAGGCT AAAAGGATTC TTGAAGATCA TGGCTCACCT 
GCTGGAGAGA TTGATGATGA AGACAAAGAC AAGGATGAAA CTGAAACAGT TAAGCAGACT 
CAAACATCTG AGGTGTATGA TGGTCCCAAA AATGTAAGAT CTGATATTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGG ATGTCCAGTG TCTATTAATT TGTCTAAAGC TGAAACTCAG 
GCTTTAACTA ATTATGGAAG TGGAGAAGAT GAAAATGAGG ATGAAGAAAT GGAAGAATTT 
GAAGAAGGCC CTGTGGATGT CCAGACTTCC CTCCAGGCTA ACACTGAAGC TACTGAAGAA 
AATGAACATG ATGAACAGGT CCTACAACGT GACTTTAAAA AGACAGCAGA AAGCAAAAAT 
GTCCCATTGG AACGAGAAGC CACTAGTAAA AATGACCAAA ATAACTGTCC TGTGAAACCC 
TGTTACCTCA ATATCTTGGA AGATGAGCAA CCTTTAAATA GTGCTGCCCA TAAGGAGTCA 
CCTCCTACTG TTGATTCAAC TCAACAGCCT AACCCTTTGC CGTTACGTTT ACCTGAAATG 
GAACCCTTAG TGCCTAGAGT CAAAGAAGTT AAATCTGCTC AGGAAACTCC TGAAAGCTCT 
CTGGCTGGAA GTCCTGATAC TGAATCTCCA GTGTTAGTGA ATGACTATGA AGCAGAATCT 
GGTAATATAA GTCAAAAGTC TGATGAAGAA GATTTTGTAA AAGTTGAAGA TTTACCACTG 
AAACTGACAA TATATTCAGA GGCAGATCTA AGAAAGAAAA TGGTAGAAGA AGAACAGAAA 
AACCATTTAT CTGGTGAAAT ATGTGAAATG CAGACCGAAG AATTAGCTGG AAATTCTGAG 
ACACTAAAAG AACCTGAAAC GGTGGGAGCC CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339568.1
CDS	423..6344
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352639.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352639.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGATTTTTC TCAGGAGCAC 
ATGGATGAAG TATGCTCCTC GCAGCTTCTA ACTTCAGTAA GGCGCATGGT TTTGACCCTT 
ACCCAGCAAA ATGATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTC AATGAATTGG CTTTCTTTAA GCTTATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCAACT 
GGAGTGATAC AATCTTGTGC CAAAGAGGCT AAAAGGATTC TTGAAGATCA TGGCTCACCT 
GCTGGAGAGA TTGATGATGA AGACAAAGAC AAGGATGAAA CTGAAACAGT TAAGCAGACT 
CAAACATCTG AGGTGTATGA TGGTCCCAAA AATGTAAGAT CTGATATTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGG ATGTCCAGTG TCTATTAATT TGTCTAAAGC TGAAACTCAG 
GCTTTAACTA ATTATGGAAG TGGAGAAGAT GAAAATGAGG ATGAAGAAAT GGAAGAATTT 
GAAGAAGGCC CTGTGGATGT CCAGACTTCC CTCCAGGCTA ACACTGAAGC TACTGAAGAA 
AATGAACATG ATGAACAGGT CCTACAACGT GACTTTAAAA AGACAGCAGA AAGCAAAAAT 
GTCCCATTGG AACGAGAAGC CACTAGTAAA AATGACCAAA ATAACTGTCC TGTGAAACCC 
TGTTACCTCA ATATCTTGGA AGATGAGCAA CCTTTAAATA GTGCTGCCCA TAAGGAGTCA 
CCTCCTACTG TTGATTCAAC TCAACAGCCT AACCCTTTGC CGTTACGTTT ACCTGAAATG 
GAACCCTTAG TGCCTAGAGT CAAAGAAGTT AAATCTGCTC AGGAAACTCC TGAAAGCTCT 
CTGGCTGGAA GTCCTGATAC TGAATCTCCA GTGTTAGTGA ATGACTATGA AGCAGAATCT 
GGTAATATAA GTCAAAAGTC TGATGAAGAA GATTTTGTAA AAGTTGAAGA TTTACCACTG 
AAACTGACAA TATATTCAGA GGCAGATCTA AGAAAGAAAA TGGTAGAAGA AGAACAGAAA 
AACCATTTAT CTGGTGAAAT ATGTGAAATG CAGACCGAAG AATTAGCTGG AAATTCTGAG 
ACACTAAAAG AACCTGAAAC GGTGGGAGCC CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352643.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339572.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 15, mRNA.

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CloneID	OHu103166
Clone ID Related Accession (Same CDS sequence)	NM_001352643.1
Accession Version	NM_001352643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 15
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013503.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.97789.1, SRR1803613.146740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGTA TTCATCCAAG AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339572.1
CDS	423..4970
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	NM_001352643.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352643.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGTA TTCATCCAAG AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352648.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339577.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 20, mRNA.

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CloneID	OHu103167
Clone ID Related Accession (Same CDS sequence)	NM_001352648.1
Accession Version	NM_001352648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 18
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013499.1. Transcript Variant: This variant (20), as well as variant 21, encodes isoform 18. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.157260.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339577.1
CDS	760..6648
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352648.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352648.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352645.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339574.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 17, mRNA.

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CloneID	OHu103169
Clone ID Related Accession (Same CDS sequence)	NM_001352645.1
Accession Version	NM_001352645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 16
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013495.1. Transcript Variant: This variant (17), as well as variant 16, encodes isoform 16. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.13490.1, SRR1803613.135289.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339574.1
CDS	760..6669
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352645.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352645.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352646.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339575.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 18, mRNA.

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CloneID	OHu103172
Clone ID Related Accession (Same CDS sequence)	NM_001352646.1 , NM_001352647.1
Accession Version	NM_001352646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 17
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013500.1. Transcript Variant: This variant (18), as well as variant 19, encodes isoform 17. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.248542.1, SRR1660805.170938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CCTCGCAGCT TCTAACTTCA 
GTAAGGCGCA TGGTTTTGAC CCTTACCCAG CAAAATGATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTCAATGAA 
TTGGCTTTCT TTAAGCTTAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC AACTGGAGTG ATACAATCTT GTGCCAAAGA GGCTAAAAGG 
ATTCTTGAAG ATCATGGCTC ACCTGCTGGA GAGATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCA GACTCAAACA TCTGAGGTGT ATGATGGTCC CAAAAATGTA 
AGATCTGATA TTTCTGATCA AGAGGAAGAT GAAGAAAGTG AAGGATGTCC AGTGTCTATT 
AATTTGTCTA AAGCTGAAAC TCAGGCTTTA ACTAATTATG GAAGTGGAGA AGATGAAAAT 
GAGGATGAAG AAATGGAAGA ATTTGAAGAA GGCCCTGTGG ATGTCCAGAC TTCCCTCCAG 
GCTAACACTG AAGCTACTGA AGAAAATGAA CATGATGAAC AGGTCCTACA ACGTGACTTT 
AAAAAGACAG CAGAAAGCAA AAATGTCCCA TTGGAACGAG AAGCCACTAG TAAAAATGAC 
CAAAATAACT GTCCTGTGAA ACCCTGTTAC CTCAATATCT TGGAAGATGA GCAACCTTTA 
AATAGTGCTG CCCATAAGGA GTCACCTCCT ACTGTTGATT CAACTCAACA GCCTAACCCT 
TTGCCGTTAC GTTTACCTGA AATGGAACCC TTAGTGCCTA GAGTCAAAGA AGTTAAATCT 
GCTCAGGAAA CTCCTGAAAG CTCTCTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAT ATAAGTCAAA AGTCTGATGA AGAAGATTTT 
GTAAAAGTTG AAGATTTACC ACTGAAACTG ACAATATATT CAGAGGCAGA TCTAAGAAAG 
AAAATGGTAG AAGAAGAACA GAAAAACCAT TTATCTGGTG AAATATGTGA AATGCAGACC 
GAAGAATTAG CTGGAAATTC TGAGACACTA AAAGAACCTG AAACGGTGGG AGCCCAGAGT 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339575.1
CDS	423..6308
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352646.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352646.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CCTCGCAGCT TCTAACTTCA 
GTAAGGCGCA TGGTTTTGAC CCTTACCCAG CAAAATGATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTCAATGAA 
TTGGCTTTCT TTAAGCTTAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC AACTGGAGTG ATACAATCTT GTGCCAAAGA GGCTAAAAGG 
ATTCTTGAAG ATCATGGCTC ACCTGCTGGA GAGATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCA GACTCAAACA TCTGAGGTGT ATGATGGTCC CAAAAATGTA 
AGATCTGATA TTTCTGATCA AGAGGAAGAT GAAGAAAGTG AAGGATGTCC AGTGTCTATT 
AATTTGTCTA AAGCTGAAAC TCAGGCTTTA ACTAATTATG GAAGTGGAGA AGATGAAAAT 
GAGGATGAAG AAATGGAAGA ATTTGAAGAA GGCCCTGTGG ATGTCCAGAC TTCCCTCCAG 
GCTAACACTG AAGCTACTGA AGAAAATGAA CATGATGAAC AGGTCCTACA ACGTGACTTT 
AAAAAGACAG CAGAAAGCAA AAATGTCCCA TTGGAACGAG AAGCCACTAG TAAAAATGAC 
CAAAATAACT GTCCTGTGAA ACCCTGTTAC CTCAATATCT TGGAAGATGA GCAACCTTTA 
AATAGTGCTG CCCATAAGGA GTCACCTCCT ACTGTTGATT CAACTCAACA GCCTAACCCT 
TTGCCGTTAC GTTTACCTGA AATGGAACCC TTAGTGCCTA GAGTCAAAGA AGTTAAATCT 
GCTCAGGAAA CTCCTGAAAG CTCTCTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAT ATAAGTCAAA AGTCTGATGA AGAAGATTTT 
GTAAAAGTTG AAGATTTACC ACTGAAACTG ACAATATATT CAGAGGCAGA TCTAAGAAAG 
AAAATGGTAG AAGAAGAACA GAAAAACCAT TTATCTGGTG AAATATGTGA AATGCAGACC 
GAAGAATTAG CTGGAAATTC TGAGACACTA AAAGAACCTG AAACGGTGGG AGCCCAGAGT 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352647.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339576.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 19, mRNA.

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CloneID	OHu103172
Clone ID Related Accession (Same CDS sequence)	NM_001352646.1 , NM_001352647.1
Accession Version	NM_001352647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 17
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013502.1. Transcript Variant: This variant (19), as well as variant 18, encodes isoform 17. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.224478.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CCTCGCAGCT TCTAACTTCA 
GTAAGGCGCA TGGTTTTGAC CCTTACCCAG CAAAATGATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTCAATGAA 
TTGGCTTTCT TTAAGCTTAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC AACTGGAGTG ATACAATCTT GTGCCAAAGA GGCTAAAAGG 
ATTCTTGAAG ATCATGGCTC ACCTGCTGGA GAGATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCA GACTCAAACA TCTGAGGTGT ATGATGGTCC CAAAAATGTA 
AGATCTGATA TTTCTGATCA AGAGGAAGAT GAAGAAAGTG AAGGATGTCC AGTGTCTATT 
AATTTGTCTA AAGCTGAAAC TCAGGCTTTA ACTAATTATG GAAGTGGAGA AGATGAAAAT 
GAGGATGAAG AAATGGAAGA ATTTGAAGAA GGCCCTGTGG ATGTCCAGAC TTCCCTCCAG 
GCTAACACTG AAGCTACTGA AGAAAATGAA CATGATGAAC AGGTCCTACA ACGTGACTTT 
AAAAAGACAG CAGAAAGCAA AAATGTCCCA TTGGAACGAG AAGCCACTAG TAAAAATGAC 
CAAAATAACT GTCCTGTGAA ACCCTGTTAC CTCAATATCT TGGAAGATGA GCAACCTTTA 
AATAGTGCTG CCCATAAGGA GTCACCTCCT ACTGTTGATT CAACTCAACA GCCTAACCCT 
TTGCCGTTAC GTTTACCTGA AATGGAACCC TTAGTGCCTA GAGTCAAAGA AGTTAAATCT 
GCTCAGGAAA CTCCTGAAAG CTCTCTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAT ATAAGTCAAA AGTCTGATGA AGAAGATTTT 
GTAAAAGTTG AAGATTTACC ACTGAAACTG ACAATATATT CAGAGGCAGA TCTAAGAAAG 
AAAATGGTAG AAGAAGAACA GAAAAACCAT TTATCTGGTG AAATATGTGA AATGCAGACC 
GAAGAATTAG CTGGAAATTC TGAGACACTA AAAGAACCTG AAACGGTGGG AGCCCAGAGT 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339576.1
CDS	355..6240
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352647.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352647.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CCTCGCAGCT TCTAACTTCA 
GTAAGGCGCA TGGTTTTGAC CCTTACCCAG CAAAATGATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTCAATGAA 
TTGGCTTTCT TTAAGCTTAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC AACTGGAGTG ATACAATCTT GTGCCAAAGA GGCTAAAAGG 
ATTCTTGAAG ATCATGGCTC ACCTGCTGGA GAGATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCA GACTCAAACA TCTGAGGTGT ATGATGGTCC CAAAAATGTA 
AGATCTGATA TTTCTGATCA AGAGGAAGAT GAAGAAAGTG AAGGATGTCC AGTGTCTATT 
AATTTGTCTA AAGCTGAAAC TCAGGCTTTA ACTAATTATG GAAGTGGAGA AGATGAAAAT 
GAGGATGAAG AAATGGAAGA ATTTGAAGAA GGCCCTGTGG ATGTCCAGAC TTCCCTCCAG 
GCTAACACTG AAGCTACTGA AGAAAATGAA CATGATGAAC AGGTCCTACA ACGTGACTTT 
AAAAAGACAG CAGAAAGCAA AAATGTCCCA TTGGAACGAG AAGCCACTAG TAAAAATGAC 
CAAAATAACT GTCCTGTGAA ACCCTGTTAC CTCAATATCT TGGAAGATGA GCAACCTTTA 
AATAGTGCTG CCCATAAGGA GTCACCTCCT ACTGTTGATT CAACTCAACA GCCTAACCCT 
TTGCCGTTAC GTTTACCTGA AATGGAACCC TTAGTGCCTA GAGTCAAAGA AGTTAAATCT 
GCTCAGGAAA CTCCTGAAAG CTCTCTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAT ATAAGTCAAA AGTCTGATGA AGAAGATTTT 
GTAAAAGTTG AAGATTTACC ACTGAAACTG ACAATATATT CAGAGGCAGA TCTAAGAAAG 
AAAATGGTAG AAGAAGAACA GAAAAACCAT TTATCTGGTG AAATATGTGA AATGCAGACC 
GAAGAATTAG CTGGAAATTC TGAGACACTA AAAGAACCTG AAACGGTGGG AGCCCAGAGT 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352642.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339571.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 14, mRNA.

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CloneID	OHu103175
Clone ID Related Accession (Same CDS sequence)	NM_001352642.1
Accession Version	NM_001352642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 14
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.123567.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGAAG CAGAATCTGG TAATATAAGT CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA 
GTTGAAGATT TACCACTGAA ACTGACAATA TATTCAGAGG CAGATCTAAG AAAGAAAATG 
GTAGAAGAAG AACAGAAAAA CCATTTATCT GGTGAAATAT GTGAAATGCA GACCGAAGAA 
TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339571.1
CDS	760..6339
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352642.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352642.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGAAG CAGAATCTGG TAATATAAGT CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA 
GTTGAAGATT TACCACTGAA ACTGACAATA TATTCAGAGG CAGATCTAAG AAAGAAAATG 
GTAGAAGAAG AACAGAAAAA CCATTTATCT GGTGAAATAT GTGAAATGCA GACCGAAGAA 
TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352642.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339571.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 14, mRNA.

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CloneID	OHu108289
Clone ID Related Accession (Same CDS sequence)	NM_001352642.2
Accession Version	NM_001352642.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 14
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352642.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.123567.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGAAG CAGAATCTGG TAATATAAGT CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA 
GTTGAAGATT TACCACTGAA ACTGACAATA TATTCAGAGG CAGATCTAAG AAAGAAAATG 
GTAGAAGAAG AACAGAAAAA CCATTTATCT GGTGAAATAT GTGAAATGCA GACCGAAGAA 
TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339571.2
CDS	629..6208
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352642.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352642.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGAAG CAGAATCTGG TAATATAAGT CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA 
GTTGAAGATT TACCACTGAA ACTGACAATA TATTCAGAGG CAGATCTAAG AAAGAAAATG 
GTAGAAGAAG AACAGAAAAA CCATTTATCT GGTGAAATAT GTGAAATGCA GACCGAAGAA 
TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352639.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339568.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 11, mRNA.

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CloneID	OHu98031
Clone ID Related Accession (Same CDS sequence)	NM_001352639.2 , XM_017013492.1
Accession Version	NM_001352639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 11
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352639.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.29208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGATTTTTC TCAGGAGCAC 
ATGGATGAAG TATGCTCCTC GCAGCTTCTA ACTTCAGTAA GGCGCATGGT TTTGACCCTT 
ACCCAGCAAA ATGATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTC AATGAATTGG CTTTCTTTAA GCTTATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCAACT 
GGAGTGATAC AATCTTGTGC CAAAGAGGCT AAAAGGATTC TTGAAGATCA TGGCTCACCT 
GCTGGAGAGA TTGATGATGA AGACAAAGAC AAGGATGAAA CTGAAACAGT TAAGCAGACT 
CAAACATCTG AGGTGTATGA TGGTCCCAAA AATGTAAGAT CTGATATTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGG ATGTCCAGTG TCTATTAATT TGTCTAAAGC TGAAACTCAG 
GCTTTAACTA ATTATGGAAG TGGAGAAGAT GAAAATGAGG ATGAAGAAAT GGAAGAATTT 
GAAGAAGGCC CTGTGGATGT CCAGACTTCC CTCCAGGCTA ACACTGAAGC TACTGAAGAA 
AATGAACATG ATGAACAGGT CCTACAACGT GACTTTAAAA AGACAGCAGA AAGCAAAAAT 
GTCCCATTGG AACGAGAAGC CACTAGTAAA AATGACCAAA ATAACTGTCC TGTGAAACCC 
TGTTACCTCA ATATCTTGGA AGATGAGCAA CCTTTAAATA GTGCTGCCCA TAAGGAGTCA 
CCTCCTACTG TTGATTCAAC TCAACAGCCT AACCCTTTGC CGTTACGTTT ACCTGAAATG 
GAACCCTTAG TGCCTAGAGT CAAAGAAGTT AAATCTGCTC AGGAAACTCC TGAAAGCTCT 
CTGGCTGGAA GTCCTGATAC TGAATCTCCA GTGTTAGTGA ATGACTATGA AGCAGAATCT 
GGTAATATAA GTCAAAAGTC TGATGAAGAA GATTTTGTAA AAGTTGAAGA TTTACCACTG 
AAACTGACAA TATATTCAGA GGCAGATCTA AGAAAGAAAA TGGTAGAAGA AGAACAGAAA 
AACCATTTAT CTGGTGAAAT ATGTGAAATG CAGACCGAAG AATTAGCTGG AAATTCTGAG 
ACACTAAAAG AACCTGAAAC GGTGGGAGCC CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339568.2
CDS	292..6213
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352639.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352639.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGATTTTTC TCAGGAGCAC 
ATGGATGAAG TATGCTCCTC GCAGCTTCTA ACTTCAGTAA GGCGCATGGT TTTGACCCTT 
ACCCAGCAAA ATGATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTC AATGAATTGG CTTTCTTTAA GCTTATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCAACT 
GGAGTGATAC AATCTTGTGC CAAAGAGGCT AAAAGGATTC TTGAAGATCA TGGCTCACCT 
GCTGGAGAGA TTGATGATGA AGACAAAGAC AAGGATGAAA CTGAAACAGT TAAGCAGACT 
CAAACATCTG AGGTGTATGA TGGTCCCAAA AATGTAAGAT CTGATATTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGG ATGTCCAGTG TCTATTAATT TGTCTAAAGC TGAAACTCAG 
GCTTTAACTA ATTATGGAAG TGGAGAAGAT GAAAATGAGG ATGAAGAAAT GGAAGAATTT 
GAAGAAGGCC CTGTGGATGT CCAGACTTCC CTCCAGGCTA ACACTGAAGC TACTGAAGAA 
AATGAACATG ATGAACAGGT CCTACAACGT GACTTTAAAA AGACAGCAGA AAGCAAAAAT 
GTCCCATTGG AACGAGAAGC CACTAGTAAA AATGACCAAA ATAACTGTCC TGTGAAACCC 
TGTTACCTCA ATATCTTGGA AGATGAGCAA CCTTTAAATA GTGCTGCCCA TAAGGAGTCA 
CCTCCTACTG TTGATTCAAC TCAACAGCCT AACCCTTTGC CGTTACGTTT ACCTGAAATG 
GAACCCTTAG TGCCTAGAGT CAAAGAAGTT AAATCTGCTC AGGAAACTCC TGAAAGCTCT 
CTGGCTGGAA GTCCTGATAC TGAATCTCCA GTGTTAGTGA ATGACTATGA AGCAGAATCT 
GGTAATATAA GTCAAAAGTC TGATGAAGAA GATTTTGTAA AAGTTGAAGA TTTACCACTG 
AAACTGACAA TATATTCAGA GGCAGATCTA AGAAAGAAAA TGGTAGAAGA AGAACAGAAA 
AACCATTTAT CTGGTGAAAT ATGTGAAATG CAGACCGAAG AATTAGCTGG AAATTCTGAG 
ACACTAAAAG AACCTGAAAC GGTGGGAGCC CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352644.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339573.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 16, mRNA.

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CloneID	OHu98032
Clone ID Related Accession (Same CDS sequence)	XM_017013495.1 , XM_017013496.1 , XM_017013494.1 , NM_001352644.2 , XM_017013496.2 , NM_001352645.2
Accession Version	NM_001352644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 16
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352644.1. Transcript Variant: This variant (16), as well as variant 17, encodes isoform 16. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.121961.1, SRR1803611.181152.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339573.2
CDS	292..6201
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352644.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352644.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352645.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339574.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 17, mRNA.

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CloneID	OHu98032
Clone ID Related Accession (Same CDS sequence)	XM_017013495.1 , XM_017013496.1 , XM_017013494.1 , NM_001352644.2 , XM_017013496.2 , NM_001352645.2
Accession Version	NM_001352645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 16
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352645.1. Transcript Variant: This variant (17), as well as variant 16, encodes isoform 16. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.13490.1, SRR1803613.246568.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339574.2
CDS	629..6538
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352645.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352645.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352641.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339570.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 13, mRNA.

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CloneID	OHu98033
Clone ID Related Accession (Same CDS sequence)	NM_001352641.2 , XM_017013497.1
Accession Version	NM_001352641.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 13
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352641.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.111685.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA TTTTTCTCAG GAGCACATGG ATGAAGTATG CTCCTCGCAG 
CTTCTAACTT CAGTAAGGCG CATGGTTTTG ACCCTTACCC AGCAAAATGA TGAGAGCAAA 
GAGTTTGTAA AGTTCTTTCA TAAACAACTT GGAAGTATAT TACAGGATTC ACTGGCAAAA 
TTTGCTGGCA GAAAACTGAA AGACTGTGGA GAAGATCTTC TTGTAGAGAT ATCTGAAGTG 
TTGTTCAATG AATTGGCTTT CTTTAAGCTT ATGCAAGATT TGGATAATAA TAGTATAACT 
GTTAAACAGA GATGCAAAAG GAAAATAGAA GCAACTGGAG TGATACAATC TTGTGCCAAA 
GAGGCTAAAA GGATTCTTGA AGATCATGGC TCACCTGCTG GAGAGATTGA TGATGAAGAC 
AAAGACAAGG ATGAAACTGA AACAGTTAAG CAGACTCAAA CATCTGAGGT GTATGATGGT 
CCCAAAAATG TAAGATCTGA TATTTCTGAT CAAGAGGAAG ATGAAGAAAG TGAAGGATGT 
CCAGTGTCTA TTAATTTGTC TAAAGCTGAA ACTCAGGCTT TAACTAATTA TGGAAGTGGA 
GAAGATGAAA ATGAGGATGA AGAAATGGAA GAATTTGAAG AAGGCCCTGT GGATGTCCAG 
ACTTCCCTCC AGGCTAACAC TGAAGCTACT GAAGAAAATG AACATGATGA ACAGGTCCTA 
CAACGTGACT TTAAAAAGAC AGCAGAAAGC AAAAATGTCC CATTGGAACG AGAAGCCACT 
AGTAAAAATG ACCAAAATAA CTGTCCTGTG AAACCCTGTT ACCTCAATAT CTTGGAAGAT 
GAGCAACCTT TAAATAGTGC TGCCCATAAG GAGTCACCTC CTACTGTTGA TTCAACTCAA 
CAGCCTAACC CTTTGCCGTT ACGTTTACCT GAAATGGAAC CCTTAGTGCC TAGAGTCAAA 
GAAGTTAAAT CTGCTCAGGA AACTCCTGAA AGCTCTCTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ATATAAGTCA AAAGTCTGAT 
GAAGAAGATT TTGTAAAAGT TGAAGATTTA CCACTGAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGAA AGAAAATGGT AGAAGAAGAA CAGAAAAACC ATTTATCTGG TGAAATATGT 
GAAATGCAGA CCGAAGAATT AGCTGGAAAT TCTGAGACAC TAAAAGAACC TGAAACGGTG 
GGAGCCCAGA GTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339570.2
CDS	292..6189
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352641.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352641.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA TTTTTCTCAG GAGCACATGG ATGAAGTATG CTCCTCGCAG 
CTTCTAACTT CAGTAAGGCG CATGGTTTTG ACCCTTACCC AGCAAAATGA TGAGAGCAAA 
GAGTTTGTAA AGTTCTTTCA TAAACAACTT GGAAGTATAT TACAGGATTC ACTGGCAAAA 
TTTGCTGGCA GAAAACTGAA AGACTGTGGA GAAGATCTTC TTGTAGAGAT ATCTGAAGTG 
TTGTTCAATG AATTGGCTTT CTTTAAGCTT ATGCAAGATT TGGATAATAA TAGTATAACT 
GTTAAACAGA GATGCAAAAG GAAAATAGAA GCAACTGGAG TGATACAATC TTGTGCCAAA 
GAGGCTAAAA GGATTCTTGA AGATCATGGC TCACCTGCTG GAGAGATTGA TGATGAAGAC 
AAAGACAAGG ATGAAACTGA AACAGTTAAG CAGACTCAAA CATCTGAGGT GTATGATGGT 
CCCAAAAATG TAAGATCTGA TATTTCTGAT CAAGAGGAAG ATGAAGAAAG TGAAGGATGT 
CCAGTGTCTA TTAATTTGTC TAAAGCTGAA ACTCAGGCTT TAACTAATTA TGGAAGTGGA 
GAAGATGAAA ATGAGGATGA AGAAATGGAA GAATTTGAAG AAGGCCCTGT GGATGTCCAG 
ACTTCCCTCC AGGCTAACAC TGAAGCTACT GAAGAAAATG AACATGATGA ACAGGTCCTA 
CAACGTGACT TTAAAAAGAC AGCAGAAAGC AAAAATGTCC CATTGGAACG AGAAGCCACT 
AGTAAAAATG ACCAAAATAA CTGTCCTGTG AAACCCTGTT ACCTCAATAT CTTGGAAGAT 
GAGCAACCTT TAAATAGTGC TGCCCATAAG GAGTCACCTC CTACTGTTGA TTCAACTCAA 
CAGCCTAACC CTTTGCCGTT ACGTTTACCT GAAATGGAAC CCTTAGTGCC TAGAGTCAAA 
GAAGTTAAAT CTGCTCAGGA AACTCCTGAA AGCTCTCTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ATATAAGTCA AAAGTCTGAT 
GAAGAAGATT TTGTAAAAGT TGAAGATTTA CCACTGAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGAA AGAAAATGGT AGAAGAAGAA CAGAAAAACC ATTTATCTGG TGAAATATGT 
GAAATGCAGA CCGAAGAATT AGCTGGAAAT TCTGAGACAC TAAAAGAACC TGAAACGGTG 
GGAGCCCAGA GTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352648.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339577.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 20, mRNA.

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CloneID	OHu98034
Clone ID Related Accession (Same CDS sequence)	XM_017013498.1 , NM_001352648.2 , XM_017013499.1 , NM_001352649.2
Accession Version	NM_001352648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 18
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352648.1. Transcript Variant: This variant (20), as well as variant 21, encodes isoform 18. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.157260.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339577.2
CDS	629..6517
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352648.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352648.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352649.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339578.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 21, mRNA.

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CloneID	OHu98034
Clone ID Related Accession (Same CDS sequence)	XM_017013498.1 , NM_001352648.2 , XM_017013499.1 , NM_001352649.2
Accession Version	NM_001352649.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 18
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352649.1. Transcript Variant: This variant (21), as well as variant 20, encodes isoform 18. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.79159.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339578.2
CDS	292..6180
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352649.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352649.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352647.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339576.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 19, mRNA.

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CloneID	OHu98035
Clone ID Related Accession (Same CDS sequence)	XM_017013501.1 , NM_001352647.2 , XM_017013502.1 , XM_017013500.1 , NM_001352646.2
Accession Version	NM_001352647.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 17
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352647.1. Transcript Variant: This variant (19), as well as variant 18, encodes isoform 17. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.224478.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CCTCGCAGCT TCTAACTTCA 
GTAAGGCGCA TGGTTTTGAC CCTTACCCAG CAAAATGATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTCAATGAA 
TTGGCTTTCT TTAAGCTTAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC AACTGGAGTG ATACAATCTT GTGCCAAAGA GGCTAAAAGG 
ATTCTTGAAG ATCATGGCTC ACCTGCTGGA GAGATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCA GACTCAAACA TCTGAGGTGT ATGATGGTCC CAAAAATGTA 
AGATCTGATA TTTCTGATCA AGAGGAAGAT GAAGAAAGTG AAGGATGTCC AGTGTCTATT 
AATTTGTCTA AAGCTGAAAC TCAGGCTTTA ACTAATTATG GAAGTGGAGA AGATGAAAAT 
GAGGATGAAG AAATGGAAGA ATTTGAAGAA GGCCCTGTGG ATGTCCAGAC TTCCCTCCAG 
GCTAACACTG AAGCTACTGA AGAAAATGAA CATGATGAAC AGGTCCTACA ACGTGACTTT 
AAAAAGACAG CAGAAAGCAA AAATGTCCCA TTGGAACGAG AAGCCACTAG TAAAAATGAC 
CAAAATAACT GTCCTGTGAA ACCCTGTTAC CTCAATATCT TGGAAGATGA GCAACCTTTA 
AATAGTGCTG CCCATAAGGA GTCACCTCCT ACTGTTGATT CAACTCAACA GCCTAACCCT 
TTGCCGTTAC GTTTACCTGA AATGGAACCC TTAGTGCCTA GAGTCAAAGA AGTTAAATCT 
GCTCAGGAAA CTCCTGAAAG CTCTCTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAT ATAAGTCAAA AGTCTGATGA AGAAGATTTT 
GTAAAAGTTG AAGATTTACC ACTGAAACTG ACAATATATT CAGAGGCAGA TCTAAGAAAG 
AAAATGGTAG AAGAAGAACA GAAAAACCAT TTATCTGGTG AAATATGTGA AATGCAGACC 
GAAGAATTAG CTGGAAATTC TGAGACACTA AAAGAACCTG AAACGGTGGG AGCCCAGAGT 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339576.2
CDS	224..6109
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352647.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352647.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CCTCGCAGCT TCTAACTTCA 
GTAAGGCGCA TGGTTTTGAC CCTTACCCAG CAAAATGATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTCAATGAA 
TTGGCTTTCT TTAAGCTTAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC AACTGGAGTG ATACAATCTT GTGCCAAAGA GGCTAAAAGG 
ATTCTTGAAG ATCATGGCTC ACCTGCTGGA GAGATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCA GACTCAAACA TCTGAGGTGT ATGATGGTCC CAAAAATGTA 
AGATCTGATA TTTCTGATCA AGAGGAAGAT GAAGAAAGTG AAGGATGTCC AGTGTCTATT 
AATTTGTCTA AAGCTGAAAC TCAGGCTTTA ACTAATTATG GAAGTGGAGA AGATGAAAAT 
GAGGATGAAG AAATGGAAGA ATTTGAAGAA GGCCCTGTGG ATGTCCAGAC TTCCCTCCAG 
GCTAACACTG AAGCTACTGA AGAAAATGAA CATGATGAAC AGGTCCTACA ACGTGACTTT 
AAAAAGACAG CAGAAAGCAA AAATGTCCCA TTGGAACGAG AAGCCACTAG TAAAAATGAC 
CAAAATAACT GTCCTGTGAA ACCCTGTTAC CTCAATATCT TGGAAGATGA GCAACCTTTA 
AATAGTGCTG CCCATAAGGA GTCACCTCCT ACTGTTGATT CAACTCAACA GCCTAACCCT 
TTGCCGTTAC GTTTACCTGA AATGGAACCC TTAGTGCCTA GAGTCAAAGA AGTTAAATCT 
GCTCAGGAAA CTCCTGAAAG CTCTCTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAT ATAAGTCAAA AGTCTGATGA AGAAGATTTT 
GTAAAAGTTG AAGATTTACC ACTGAAACTG ACAATATATT CAGAGGCAGA TCTAAGAAAG 
AAAATGGTAG AAGAAGAACA GAAAAACCAT TTATCTGGTG AAATATGTGA AATGCAGACC 
GAAGAATTAG CTGGAAATTC TGAGACACTA AAAGAACCTG AAACGGTGGG AGCCCAGAGT 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352646.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339575.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 18, mRNA.

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CloneID	OHu98035
Clone ID Related Accession (Same CDS sequence)	XM_017013501.1 , NM_001352647.2 , XM_017013502.1 , XM_017013500.1 , NM_001352646.2
Accession Version	NM_001352646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 17
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352646.1. Transcript Variant: This variant (18), as well as variant 19, encodes isoform 17. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.248542.1, SRR1660805.170938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CCTCGCAGCT TCTAACTTCA 
GTAAGGCGCA TGGTTTTGAC CCTTACCCAG CAAAATGATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTCAATGAA 
TTGGCTTTCT TTAAGCTTAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC AACTGGAGTG ATACAATCTT GTGCCAAAGA GGCTAAAAGG 
ATTCTTGAAG ATCATGGCTC ACCTGCTGGA GAGATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCA GACTCAAACA TCTGAGGTGT ATGATGGTCC CAAAAATGTA 
AGATCTGATA TTTCTGATCA AGAGGAAGAT GAAGAAAGTG AAGGATGTCC AGTGTCTATT 
AATTTGTCTA AAGCTGAAAC TCAGGCTTTA ACTAATTATG GAAGTGGAGA AGATGAAAAT 
GAGGATGAAG AAATGGAAGA ATTTGAAGAA GGCCCTGTGG ATGTCCAGAC TTCCCTCCAG 
GCTAACACTG AAGCTACTGA AGAAAATGAA CATGATGAAC AGGTCCTACA ACGTGACTTT 
AAAAAGACAG CAGAAAGCAA AAATGTCCCA TTGGAACGAG AAGCCACTAG TAAAAATGAC 
CAAAATAACT GTCCTGTGAA ACCCTGTTAC CTCAATATCT TGGAAGATGA GCAACCTTTA 
AATAGTGCTG CCCATAAGGA GTCACCTCCT ACTGTTGATT CAACTCAACA GCCTAACCCT 
TTGCCGTTAC GTTTACCTGA AATGGAACCC TTAGTGCCTA GAGTCAAAGA AGTTAAATCT 
GCTCAGGAAA CTCCTGAAAG CTCTCTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAT ATAAGTCAAA AGTCTGATGA AGAAGATTTT 
GTAAAAGTTG AAGATTTACC ACTGAAACTG ACAATATATT CAGAGGCAGA TCTAAGAAAG 
AAAATGGTAG AAGAAGAACA GAAAAACCAT TTATCTGGTG AAATATGTGA AATGCAGACC 
GAAGAATTAG CTGGAAATTC TGAGACACTA AAAGAACCTG AAACGGTGGG AGCCCAGAGT 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339575.2
CDS	292..6177
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352646.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352646.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CCTCGCAGCT TCTAACTTCA 
GTAAGGCGCA TGGTTTTGAC CCTTACCCAG CAAAATGATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTCAATGAA 
TTGGCTTTCT TTAAGCTTAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC AACTGGAGTG ATACAATCTT GTGCCAAAGA GGCTAAAAGG 
ATTCTTGAAG ATCATGGCTC ACCTGCTGGA GAGATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCA GACTCAAACA TCTGAGGTGT ATGATGGTCC CAAAAATGTA 
AGATCTGATA TTTCTGATCA AGAGGAAGAT GAAGAAAGTG AAGGATGTCC AGTGTCTATT 
AATTTGTCTA AAGCTGAAAC TCAGGCTTTA ACTAATTATG GAAGTGGAGA AGATGAAAAT 
GAGGATGAAG AAATGGAAGA ATTTGAAGAA GGCCCTGTGG ATGTCCAGAC TTCCCTCCAG 
GCTAACACTG AAGCTACTGA AGAAAATGAA CATGATGAAC AGGTCCTACA ACGTGACTTT 
AAAAAGACAG CAGAAAGCAA AAATGTCCCA TTGGAACGAG AAGCCACTAG TAAAAATGAC 
CAAAATAACT GTCCTGTGAA ACCCTGTTAC CTCAATATCT TGGAAGATGA GCAACCTTTA 
AATAGTGCTG CCCATAAGGA GTCACCTCCT ACTGTTGATT CAACTCAACA GCCTAACCCT 
TTGCCGTTAC GTTTACCTGA AATGGAACCC TTAGTGCCTA GAGTCAAAGA AGTTAAATCT 
GCTCAGGAAA CTCCTGAAAG CTCTCTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAT ATAAGTCAAA AGTCTGATGA AGAAGATTTT 
GTAAAAGTTG AAGATTTACC ACTGAAACTG ACAATATATT CAGAGGCAGA TCTAAGAAAG 
AAAATGGTAG AAGAAGAACA GAAAAACCAT TTATCTGGTG AAATATGTGA AATGCAGACC 
GAAGAATTAG CTGGAAATTC TGAGACACTA AAAGAACCTG AAACGGTGGG AGCCCAGAGT 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352643.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339572.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 15, mRNA.

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CloneID	OHu98036
Clone ID Related Accession (Same CDS sequence)	XM_017013503.1 , NM_001352643.2
Accession Version	NM_001352643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 15
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352643.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.218544.1, SRR1803613.146740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGTA TTCATCCAAG AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339572.2
CDS	292..4839
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	NM_001352643.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352643.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGTA TTCATCCAAG AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013491.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868980.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X21, mRNA.

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CloneID	OHu98030
Clone ID Related Accession (Same CDS sequence)	XM_017013491.1
Accession Version	XM_017013491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGGTACTA CAGTTAATAA TTTAGAAGAA ACTCCCGTTA TTGAAAATCG TAGTTCACAA 
CAACCTGTAA GTGAAGTTTC TACCATCCCA TGTCCTAGAA TTGATACTCA GCAGCTGGAC 
CGGCAAATTA AAGCAATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGATTT TTCTCAGGAG 
CACATGGATG AAGTATGCTC CTCGCAGCTT CTAACTTCAG TAAGGCGCAT GGTTTTGACC 
CTTACCCAGC AAAATGATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA ACAACTTGGA 
AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAAGA CTGTGGAGAA 
GATCTTCTTG TAGAGATATC TGAAGTGTTG TTCAATGAAT TGGCTTTCTT TAAGCTTATG 
CAAGATTTGG ATAATAATAG TATAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCA 
ACTGGAGTGA TACAATCTTG TGCCAAAGAG GCTAAAAGGA TTCTTGAAGA TCATGGCTCA 
CCTGCTGGAG AGATTGATGA TGAAGACAAA GACAAGGATG AAACTGAAAC AGTTAAGCAG 
ACTCAAACAT CTGAGGTGTA TGATGGTCCC AAAAATGTAA GATCTGATAT TTCTGATCAA 
GAGGAAGATG AAGAAAGTGA AGGATGTCCA GTGTCTATTA ATTTGTCTAA AGCTGAAACT 
CAGGCTTTAA CTAATTATGG AAGTGGAGAA GATGAAAATG AGGATGAAGA AATGGAAGAA 
TTTGAAGAAG GCCCTGTGGA TGTCCAGACT TCCCTCCAGG CTAACACTGA AGCTACTGAA 
GAAAATGAAC ATGATGAACA GGTCCTACAA CGTGACTTTA AAAAGACAGC AGAAAGCAAA 
AATGTCCCAT TGGAACGAGA AGCCACTAGT AAAAATGACC AAAATAACTG TCCTGTGAAA 
CCCTGTTACC TCAATATCTT GGAAGATGAG CAACCTTTAA ATAGTGCTGC CCATAAGGAG 
TCACCTCCTA CTGTTGATTC AACTCAACAG CCTAACCCTT TGCCGTTACG TTTACCTGAA 
ATGGAACCCT TAGTGCCTAG AGTCAAAGAA GTTAAATCTG CTCAGGAAAC TCCTGAAAGC 
TCTCTGGCTG GAAGTCCTGA TACTGAATCT CCAGTGTTAG TGAATGACTA TGAAGCAGAA 
TCTGGTAATA TAAGTCAAAA GTCTGATGAA GAAGATTTTG TAAAAGTTGA AGATTTACCA 
CTGAAACTGA CAATATATTC AGAGGCAGAT CTAAGAAAGA AAATGGTAGA AGAAGAACAG 
AAAAACCATT TATCTGGTGA AATATGTGAA ATGCAGACCG AAGAATTAGC TGGAAATTCT 
GAGACACTAA AAGAACCTGA AACGGTGGGA GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868980.1
CDS	496..6420
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013491.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013491.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGGTACTA CAGTTAATAA TTTAGAAGAA ACTCCCGTTA TTGAAAATCG TAGTTCACAA 
CAACCTGTAA GTGAAGTTTC TACCATCCCA TGTCCTAGAA TTGATACTCA GCAGCTGGAC 
CGGCAAATTA AAGCAATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGATTT TTCTCAGGAG 
CACATGGATG AAGTATGCTC CTCGCAGCTT CTAACTTCAG TAAGGCGCAT GGTTTTGACC 
CTTACCCAGC AAAATGATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA ACAACTTGGA 
AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAAGA CTGTGGAGAA 
GATCTTCTTG TAGAGATATC TGAAGTGTTG TTCAATGAAT TGGCTTTCTT TAAGCTTATG 
CAAGATTTGG ATAATAATAG TATAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCA 
ACTGGAGTGA TACAATCTTG TGCCAAAGAG GCTAAAAGGA TTCTTGAAGA TCATGGCTCA 
CCTGCTGGAG AGATTGATGA TGAAGACAAA GACAAGGATG AAACTGAAAC AGTTAAGCAG 
ACTCAAACAT CTGAGGTGTA TGATGGTCCC AAAAATGTAA GATCTGATAT TTCTGATCAA 
GAGGAAGATG AAGAAAGTGA AGGATGTCCA GTGTCTATTA ATTTGTCTAA AGCTGAAACT 
CAGGCTTTAA CTAATTATGG AAGTGGAGAA GATGAAAATG AGGATGAAGA AATGGAAGAA 
TTTGAAGAAG GCCCTGTGGA TGTCCAGACT TCCCTCCAGG CTAACACTGA AGCTACTGAA 
GAAAATGAAC ATGATGAACA GGTCCTACAA CGTGACTTTA AAAAGACAGC AGAAAGCAAA 
AATGTCCCAT TGGAACGAGA AGCCACTAGT AAAAATGACC AAAATAACTG TCCTGTGAAA 
CCCTGTTACC TCAATATCTT GGAAGATGAG CAACCTTTAA ATAGTGCTGC CCATAAGGAG 
TCACCTCCTA CTGTTGATTC AACTCAACAG CCTAACCCTT TGCCGTTACG TTTACCTGAA 
ATGGAACCCT TAGTGCCTAG AGTCAAAGAA GTTAAATCTG CTCAGGAAAC TCCTGAAAGC 
TCTCTGGCTG GAAGTCCTGA TACTGAATCT CCAGTGTTAG TGAATGACTA TGAAGCAGAA 
TCTGGTAATA TAAGTCAAAA GTCTGATGAA GAAGATTTTG TAAAAGTTGA AGATTTACCA 
CTGAAACTGA CAATATATTC AGAGGCAGAT CTAAGAAAGA AAATGGTAGA AGAAGAACAG 
AAAAACCATT TATCTGGTGA AATATGTGAA ATGCAGACCG AAGAATTAGC TGGAAATTCT 
GAGACACTAA AAGAACCTGA AACGGTGGGA GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013492.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868981.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X31, mRNA.

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CloneID	OHu98031
Clone ID Related Accession (Same CDS sequence)	NM_001352639.2 , XM_017013492.1
Accession Version	XM_017013492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X24
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGATTTTTC TCAGGAGCAC 
ATGGATGAAG TATGCTCCTC GCAGCTTCTA ACTTCAGTAA GGCGCATGGT TTTGACCCTT 
ACCCAGCAAA ATGATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTC AATGAATTGG CTTTCTTTAA GCTTATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCAACT 
GGAGTGATAC AATCTTGTGC CAAAGAGGCT AAAAGGATTC TTGAAGATCA TGGCTCACCT 
GCTGGAGAGA TTGATGATGA AGACAAAGAC AAGGATGAAA CTGAAACAGT TAAGCAGACT 
CAAACATCTG AGGTGTATGA TGGTCCCAAA AATGTAAGAT CTGATATTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGG ATGTCCAGTG TCTATTAATT TGTCTAAAGC TGAAACTCAG 
GCTTTAACTA ATTATGGAAG TGGAGAAGAT GAAAATGAGG ATGAAGAAAT GGAAGAATTT 
GAAGAAGGCC CTGTGGATGT CCAGACTTCC CTCCAGGCTA ACACTGAAGC TACTGAAGAA 
AATGAACATG ATGAACAGGT CCTACAACGT GACTTTAAAA AGACAGCAGA AAGCAAAAAT 
GTCCCATTGG AACGAGAAGC CACTAGTAAA AATGACCAAA ATAACTGTCC TGTGAAACCC 
TGTTACCTCA ATATCTTGGA AGATGAGCAA CCTTTAAATA GTGCTGCCCA TAAGGAGTCA 
CCTCCTACTG TTGATTCAAC TCAACAGCCT AACCCTTTGC CGTTACGTTT ACCTGAAATG 
GAACCCTTAG TGCCTAGAGT CAAAGAAGTT AAATCTGCTC AGGAAACTCC TGAAAGCTCT 
CTGGCTGGAA GTCCTGATAC TGAATCTCCA GTGTTAGTGA ATGACTATGA AGCAGAATCT 
GGTAATATAA GTCAAAAGTC TGATGAAGAA GATTTTGTAA AAGTTGAAGA TTTACCACTG 
AAACTGACAA TATATTCAGA GGCAGATCTA AGAAAGAAAA TGGTAGAAGA AGAACAGAAA 
AACCATTTAT CTGGTGAAAT ATGTGAAATG CAGACCGAAG AATTAGCTGG AAATTCTGAG 
ACACTAAAAG AACCTGAAAC GGTGGGAGCC CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868981.1
CDS	496..6417
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013492.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013492.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGATTTTTC TCAGGAGCAC 
ATGGATGAAG TATGCTCCTC GCAGCTTCTA ACTTCAGTAA GGCGCATGGT TTTGACCCTT 
ACCCAGCAAA ATGATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTC AATGAATTGG CTTTCTTTAA GCTTATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCAACT 
GGAGTGATAC AATCTTGTGC CAAAGAGGCT AAAAGGATTC TTGAAGATCA TGGCTCACCT 
GCTGGAGAGA TTGATGATGA AGACAAAGAC AAGGATGAAA CTGAAACAGT TAAGCAGACT 
CAAACATCTG AGGTGTATGA TGGTCCCAAA AATGTAAGAT CTGATATTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGG ATGTCCAGTG TCTATTAATT TGTCTAAAGC TGAAACTCAG 
GCTTTAACTA ATTATGGAAG TGGAGAAGAT GAAAATGAGG ATGAAGAAAT GGAAGAATTT 
GAAGAAGGCC CTGTGGATGT CCAGACTTCC CTCCAGGCTA ACACTGAAGC TACTGAAGAA 
AATGAACATG ATGAACAGGT CCTACAACGT GACTTTAAAA AGACAGCAGA AAGCAAAAAT 
GTCCCATTGG AACGAGAAGC CACTAGTAAA AATGACCAAA ATAACTGTCC TGTGAAACCC 
TGTTACCTCA ATATCTTGGA AGATGAGCAA CCTTTAAATA GTGCTGCCCA TAAGGAGTCA 
CCTCCTACTG TTGATTCAAC TCAACAGCCT AACCCTTTGC CGTTACGTTT ACCTGAAATG 
GAACCCTTAG TGCCTAGAGT CAAAGAAGTT AAATCTGCTC AGGAAACTCC TGAAAGCTCT 
CTGGCTGGAA GTCCTGATAC TGAATCTCCA GTGTTAGTGA ATGACTATGA AGCAGAATCT 
GGTAATATAA GTCAAAAGTC TGATGAAGAA GATTTTGTAA AAGTTGAAGA TTTACCACTG 
AAACTGACAA TATATTCAGA GGCAGATCTA AGAAAGAAAA TGGTAGAAGA AGAACAGAAA 
AACCATTTAT CTGGTGAAAT ATGTGAAATG CAGACCGAAG AATTAGCTGG AAATTCTGAG 
ACACTAAAAG AACCTGAAAC GGTGGGAGCC CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013495.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868984.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X35, mRNA.

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CloneID	OHu98032
Clone ID Related Accession (Same CDS sequence)	XM_017013495.1 , XM_017013496.1 , XM_017013494.1 , NM_001352644.2 , XM_017013496.2 , NM_001352645.2
Accession Version	XM_017013495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X26
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868984.1
CDS	636..6545
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013495.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013495.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013496.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868985.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X36, mRNA.

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CloneID	OHu98032
Clone ID Related Accession (Same CDS sequence)	XM_017013495.1 , XM_017013496.1 , XM_017013494.1 , NM_001352644.2 , XM_017013496.2 , NM_001352645.2
Accession Version	XM_017013496.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868985.1
CDS	219..6128
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013496.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013496.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013494.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868983.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X34, mRNA.

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CloneID	OHu98032
Clone ID Related Accession (Same CDS sequence)	XM_017013495.1 , XM_017013496.1 , XM_017013494.1 , NM_001352644.2 , XM_017013496.2 , NM_001352645.2
Accession Version	XM_017013494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X26
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868983.1
CDS	496..6405
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013494.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013494.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013496.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868985.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X23, mRNA.

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CloneID	OHu98032
Clone ID Related Accession (Same CDS sequence)	XM_017013495.1 , XM_017013496.1 , XM_017013494.1 , NM_001352644.2 , XM_017013496.2 , NM_001352645.2
Accession Version	XM_017013496.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017013496.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868985.1
CDS	393..6302
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013496.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013496.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013497.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868986.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X37, mRNA.

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CloneID	OHu98033
Clone ID Related Accession (Same CDS sequence)	NM_001352641.2 , XM_017013497.1
Accession Version	XM_017013497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X27
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA TTTTTCTCAG GAGCACATGG ATGAAGTATG CTCCTCGCAG 
CTTCTAACTT CAGTAAGGCG CATGGTTTTG ACCCTTACCC AGCAAAATGA TGAGAGCAAA 
GAGTTTGTAA AGTTCTTTCA TAAACAACTT GGAAGTATAT TACAGGATTC ACTGGCAAAA 
TTTGCTGGCA GAAAACTGAA AGACTGTGGA GAAGATCTTC TTGTAGAGAT ATCTGAAGTG 
TTGTTCAATG AATTGGCTTT CTTTAAGCTT ATGCAAGATT TGGATAATAA TAGTATAACT 
GTTAAACAGA GATGCAAAAG GAAAATAGAA GCAACTGGAG TGATACAATC TTGTGCCAAA 
GAGGCTAAAA GGATTCTTGA AGATCATGGC TCACCTGCTG GAGAGATTGA TGATGAAGAC 
AAAGACAAGG ATGAAACTGA AACAGTTAAG CAGACTCAAA CATCTGAGGT GTATGATGGT 
CCCAAAAATG TAAGATCTGA TATTTCTGAT CAAGAGGAAG ATGAAGAAAG TGAAGGATGT 
CCAGTGTCTA TTAATTTGTC TAAAGCTGAA ACTCAGGCTT TAACTAATTA TGGAAGTGGA 
GAAGATGAAA ATGAGGATGA AGAAATGGAA GAATTTGAAG AAGGCCCTGT GGATGTCCAG 
ACTTCCCTCC AGGCTAACAC TGAAGCTACT GAAGAAAATG AACATGATGA ACAGGTCCTA 
CAACGTGACT TTAAAAAGAC AGCAGAAAGC AAAAATGTCC CATTGGAACG AGAAGCCACT 
AGTAAAAATG ACCAAAATAA CTGTCCTGTG AAACCCTGTT ACCTCAATAT CTTGGAAGAT 
GAGCAACCTT TAAATAGTGC TGCCCATAAG GAGTCACCTC CTACTGTTGA TTCAACTCAA 
CAGCCTAACC CTTTGCCGTT ACGTTTACCT GAAATGGAAC CCTTAGTGCC TAGAGTCAAA 
GAAGTTAAAT CTGCTCAGGA AACTCCTGAA AGCTCTCTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ATATAAGTCA AAAGTCTGAT 
GAAGAAGATT TTGTAAAAGT TGAAGATTTA CCACTGAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGAA AGAAAATGGT AGAAGAAGAA CAGAAAAACC ATTTATCTGG TGAAATATGT 
GAAATGCAGA CCGAAGAATT AGCTGGAAAT TCTGAGACAC TAAAAGAACC TGAAACGGTG 
GGAGCCCAGA GTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868986.1
CDS	496..6393
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013497.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013497.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA TTTTTCTCAG GAGCACATGG ATGAAGTATG CTCCTCGCAG 
CTTCTAACTT CAGTAAGGCG CATGGTTTTG ACCCTTACCC AGCAAAATGA TGAGAGCAAA 
GAGTTTGTAA AGTTCTTTCA TAAACAACTT GGAAGTATAT TACAGGATTC ACTGGCAAAA 
TTTGCTGGCA GAAAACTGAA AGACTGTGGA GAAGATCTTC TTGTAGAGAT ATCTGAAGTG 
TTGTTCAATG AATTGGCTTT CTTTAAGCTT ATGCAAGATT TGGATAATAA TAGTATAACT 
GTTAAACAGA GATGCAAAAG GAAAATAGAA GCAACTGGAG TGATACAATC TTGTGCCAAA 
GAGGCTAAAA GGATTCTTGA AGATCATGGC TCACCTGCTG GAGAGATTGA TGATGAAGAC 
AAAGACAAGG ATGAAACTGA AACAGTTAAG CAGACTCAAA CATCTGAGGT GTATGATGGT 
CCCAAAAATG TAAGATCTGA TATTTCTGAT CAAGAGGAAG ATGAAGAAAG TGAAGGATGT 
CCAGTGTCTA TTAATTTGTC TAAAGCTGAA ACTCAGGCTT TAACTAATTA TGGAAGTGGA 
GAAGATGAAA ATGAGGATGA AGAAATGGAA GAATTTGAAG AAGGCCCTGT GGATGTCCAG 
ACTTCCCTCC AGGCTAACAC TGAAGCTACT GAAGAAAATG AACATGATGA ACAGGTCCTA 
CAACGTGACT TTAAAAAGAC AGCAGAAAGC AAAAATGTCC CATTGGAACG AGAAGCCACT 
AGTAAAAATG ACCAAAATAA CTGTCCTGTG AAACCCTGTT ACCTCAATAT CTTGGAAGAT 
GAGCAACCTT TAAATAGTGC TGCCCATAAG GAGTCACCTC CTACTGTTGA TTCAACTCAA 
CAGCCTAACC CTTTGCCGTT ACGTTTACCT GAAATGGAAC CCTTAGTGCC TAGAGTCAAA 
GAAGTTAAAT CTGCTCAGGA AACTCCTGAA AGCTCTCTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ATATAAGTCA AAAGTCTGAT 
GAAGAAGATT TTGTAAAAGT TGAAGATTTA CCACTGAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGAA AGAAAATGGT AGAAGAAGAA CAGAAAAACC ATTTATCTGG TGAAATATGT 
GAAATGCAGA CCGAAGAATT AGCTGGAAAT TCTGAGACAC TAAAAGAACC TGAAACGGTG 
GGAGCCCAGA GTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013498.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868987.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X38, mRNA.

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CloneID	OHu98034
Clone ID Related Accession (Same CDS sequence)	XM_017013498.1 , NM_001352648.2 , XM_017013499.1 , NM_001352649.2
Accession Version	XM_017013498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X28
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868987.1
CDS	496..6384
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013498.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013498.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013499.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868988.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X39, mRNA.

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CloneID	OHu98034
Clone ID Related Accession (Same CDS sequence)	XM_017013498.1 , NM_001352648.2 , XM_017013499.1 , NM_001352649.2
Accession Version	XM_017013499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X28
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868988.1
CDS	636..6524
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013499.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013499.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013501.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868990.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X24, mRNA.

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CloneID	OHu98035
Clone ID Related Accession (Same CDS sequence)	XM_017013501.1 , NM_001352647.2 , XM_017013502.1 , XM_017013500.1 , NM_001352646.2
Accession Version	XM_017013501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CCTCGCAGCT TCTAACTTCA 
GTAAGGCGCA TGGTTTTGAC CCTTACCCAG CAAAATGATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTCAATGAA 
TTGGCTTTCT TTAAGCTTAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC AACTGGAGTG ATACAATCTT GTGCCAAAGA GGCTAAAAGG 
ATTCTTGAAG ATCATGGCTC ACCTGCTGGA GAGATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCA GACTCAAACA TCTGAGGTGT ATGATGGTCC CAAAAATGTA 
AGATCTGATA TTTCTGATCA AGAGGAAGAT GAAGAAAGTG AAGGATGTCC AGTGTCTATT 
AATTTGTCTA AAGCTGAAAC TCAGGCTTTA ACTAATTATG GAAGTGGAGA AGATGAAAAT 
GAGGATGAAG AAATGGAAGA ATTTGAAGAA GGCCCTGTGG ATGTCCAGAC TTCCCTCCAG 
GCTAACACTG AAGCTACTGA AGAAAATGAA CATGATGAAC AGGTCCTACA ACGTGACTTT 
AAAAAGACAG CAGAAAGCAA AAATGTCCCA TTGGAACGAG AAGCCACTAG TAAAAATGAC 
CAAAATAACT GTCCTGTGAA ACCCTGTTAC CTCAATATCT TGGAAGATGA GCAACCTTTA 
AATAGTGCTG CCCATAAGGA GTCACCTCCT ACTGTTGATT CAACTCAACA GCCTAACCCT 
TTGCCGTTAC GTTTACCTGA AATGGAACCC TTAGTGCCTA GAGTCAAAGA AGTTAAATCT 
GCTCAGGAAA CTCCTGAAAG CTCTCTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAT ATAAGTCAAA AGTCTGATGA AGAAGATTTT 
GTAAAAGTTG AAGATTTACC ACTGAAACTG ACAATATATT CAGAGGCAGA TCTAAGAAAG 
AAAATGGTAG AAGAAGAACA GAAAAACCAT TTATCTGGTG AAATATGTGA AATGCAGACC 
GAAGAATTAG CTGGAAATTC TGAGACACTA AAAGAACCTG AAACGGTGGG AGCCCAGAGT 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868990.1
CDS	798..6683
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013501.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013501.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CCTCGCAGCT TCTAACTTCA 
GTAAGGCGCA TGGTTTTGAC CCTTACCCAG CAAAATGATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTCAATGAA 
TTGGCTTTCT TTAAGCTTAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC AACTGGAGTG ATACAATCTT GTGCCAAAGA GGCTAAAAGG 
ATTCTTGAAG ATCATGGCTC ACCTGCTGGA GAGATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCA GACTCAAACA TCTGAGGTGT ATGATGGTCC CAAAAATGTA 
AGATCTGATA TTTCTGATCA AGAGGAAGAT GAAGAAAGTG AAGGATGTCC AGTGTCTATT 
AATTTGTCTA AAGCTGAAAC TCAGGCTTTA ACTAATTATG GAAGTGGAGA AGATGAAAAT 
GAGGATGAAG AAATGGAAGA ATTTGAAGAA GGCCCTGTGG ATGTCCAGAC TTCCCTCCAG 
GCTAACACTG AAGCTACTGA AGAAAATGAA CATGATGAAC AGGTCCTACA ACGTGACTTT 
AAAAAGACAG CAGAAAGCAA AAATGTCCCA TTGGAACGAG AAGCCACTAG TAAAAATGAC 
CAAAATAACT GTCCTGTGAA ACCCTGTTAC CTCAATATCT TGGAAGATGA GCAACCTTTA 
AATAGTGCTG CCCATAAGGA GTCACCTCCT ACTGTTGATT CAACTCAACA GCCTAACCCT 
TTGCCGTTAC GTTTACCTGA AATGGAACCC TTAGTGCCTA GAGTCAAAGA AGTTAAATCT 
GCTCAGGAAA CTCCTGAAAG CTCTCTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAT ATAAGTCAAA AGTCTGATGA AGAAGATTTT 
GTAAAAGTTG AAGATTTACC ACTGAAACTG ACAATATATT CAGAGGCAGA TCTAAGAAAG 
AAAATGGTAG AAGAAGAACA GAAAAACCAT TTATCTGGTG AAATATGTGA AATGCAGACC 
GAAGAATTAG CTGGAAATTC TGAGACACTA AAAGAACCTG AAACGGTGGG AGCCCAGAGT 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013502.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868991.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X42, mRNA.

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CloneID	OHu98035
Clone ID Related Accession (Same CDS sequence)	XM_017013501.1 , NM_001352647.2 , XM_017013502.1 , XM_017013500.1 , NM_001352646.2
Accession Version	XM_017013502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X29
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CCTCGCAGCT TCTAACTTCA 
GTAAGGCGCA TGGTTTTGAC CCTTACCCAG CAAAATGATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTCAATGAA 
TTGGCTTTCT TTAAGCTTAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC AACTGGAGTG ATACAATCTT GTGCCAAAGA GGCTAAAAGG 
ATTCTTGAAG ATCATGGCTC ACCTGCTGGA GAGATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCA GACTCAAACA TCTGAGGTGT ATGATGGTCC CAAAAATGTA 
AGATCTGATA TTTCTGATCA AGAGGAAGAT GAAGAAAGTG AAGGATGTCC AGTGTCTATT 
AATTTGTCTA AAGCTGAAAC TCAGGCTTTA ACTAATTATG GAAGTGGAGA AGATGAAAAT 
GAGGATGAAG AAATGGAAGA ATTTGAAGAA GGCCCTGTGG ATGTCCAGAC TTCCCTCCAG 
GCTAACACTG AAGCTACTGA AGAAAATGAA CATGATGAAC AGGTCCTACA ACGTGACTTT 
AAAAAGACAG CAGAAAGCAA AAATGTCCCA TTGGAACGAG AAGCCACTAG TAAAAATGAC 
CAAAATAACT GTCCTGTGAA ACCCTGTTAC CTCAATATCT TGGAAGATGA GCAACCTTTA 
AATAGTGCTG CCCATAAGGA GTCACCTCCT ACTGTTGATT CAACTCAACA GCCTAACCCT 
TTGCCGTTAC GTTTACCTGA AATGGAACCC TTAGTGCCTA GAGTCAAAGA AGTTAAATCT 
GCTCAGGAAA CTCCTGAAAG CTCTCTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAT ATAAGTCAAA AGTCTGATGA AGAAGATTTT 
GTAAAAGTTG AAGATTTACC ACTGAAACTG ACAATATATT CAGAGGCAGA TCTAAGAAAG 
AAAATGGTAG AAGAAGAACA GAAAAACCAT TTATCTGGTG AAATATGTGA AATGCAGACC 
GAAGAATTAG CTGGAAATTC TGAGACACTA AAAGAACCTG AAACGGTGGG AGCCCAGAGT 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868991.1
CDS	219..6104
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013502.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X42, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013502.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CCTCGCAGCT TCTAACTTCA 
GTAAGGCGCA TGGTTTTGAC CCTTACCCAG CAAAATGATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTCAATGAA 
TTGGCTTTCT TTAAGCTTAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC AACTGGAGTG ATACAATCTT GTGCCAAAGA GGCTAAAAGG 
ATTCTTGAAG ATCATGGCTC ACCTGCTGGA GAGATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCA GACTCAAACA TCTGAGGTGT ATGATGGTCC CAAAAATGTA 
AGATCTGATA TTTCTGATCA AGAGGAAGAT GAAGAAAGTG AAGGATGTCC AGTGTCTATT 
AATTTGTCTA AAGCTGAAAC TCAGGCTTTA ACTAATTATG GAAGTGGAGA AGATGAAAAT 
GAGGATGAAG AAATGGAAGA ATTTGAAGAA GGCCCTGTGG ATGTCCAGAC TTCCCTCCAG 
GCTAACACTG AAGCTACTGA AGAAAATGAA CATGATGAAC AGGTCCTACA ACGTGACTTT 
AAAAAGACAG CAGAAAGCAA AAATGTCCCA TTGGAACGAG AAGCCACTAG TAAAAATGAC 
CAAAATAACT GTCCTGTGAA ACCCTGTTAC CTCAATATCT TGGAAGATGA GCAACCTTTA 
AATAGTGCTG CCCATAAGGA GTCACCTCCT ACTGTTGATT CAACTCAACA GCCTAACCCT 
TTGCCGTTAC GTTTACCTGA AATGGAACCC TTAGTGCCTA GAGTCAAAGA AGTTAAATCT 
GCTCAGGAAA CTCCTGAAAG CTCTCTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAT ATAAGTCAAA AGTCTGATGA AGAAGATTTT 
GTAAAAGTTG AAGATTTACC ACTGAAACTG ACAATATATT CAGAGGCAGA TCTAAGAAAG 
AAAATGGTAG AAGAAGAACA GAAAAACCAT TTATCTGGTG AAATATGTGA AATGCAGACC 
GAAGAATTAG CTGGAAATTC TGAGACACTA AAAGAACCTG AAACGGTGGG AGCCCAGAGT 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013500.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868989.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X40, mRNA.

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CloneID	OHu98035
Clone ID Related Accession (Same CDS sequence)	XM_017013501.1 , NM_001352647.2 , XM_017013502.1 , XM_017013500.1 , NM_001352646.2
Accession Version	XM_017013500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X29
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CCTCGCAGCT TCTAACTTCA 
GTAAGGCGCA TGGTTTTGAC CCTTACCCAG CAAAATGATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTCAATGAA 
TTGGCTTTCT TTAAGCTTAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC AACTGGAGTG ATACAATCTT GTGCCAAAGA GGCTAAAAGG 
ATTCTTGAAG ATCATGGCTC ACCTGCTGGA GAGATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCA GACTCAAACA TCTGAGGTGT ATGATGGTCC CAAAAATGTA 
AGATCTGATA TTTCTGATCA AGAGGAAGAT GAAGAAAGTG AAGGATGTCC AGTGTCTATT 
AATTTGTCTA AAGCTGAAAC TCAGGCTTTA ACTAATTATG GAAGTGGAGA AGATGAAAAT 
GAGGATGAAG AAATGGAAGA ATTTGAAGAA GGCCCTGTGG ATGTCCAGAC TTCCCTCCAG 
GCTAACACTG AAGCTACTGA AGAAAATGAA CATGATGAAC AGGTCCTACA ACGTGACTTT 
AAAAAGACAG CAGAAAGCAA AAATGTCCCA TTGGAACGAG AAGCCACTAG TAAAAATGAC 
CAAAATAACT GTCCTGTGAA ACCCTGTTAC CTCAATATCT TGGAAGATGA GCAACCTTTA 
AATAGTGCTG CCCATAAGGA GTCACCTCCT ACTGTTGATT CAACTCAACA GCCTAACCCT 
TTGCCGTTAC GTTTACCTGA AATGGAACCC TTAGTGCCTA GAGTCAAAGA AGTTAAATCT 
GCTCAGGAAA CTCCTGAAAG CTCTCTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAT ATAAGTCAAA AGTCTGATGA AGAAGATTTT 
GTAAAAGTTG AAGATTTACC ACTGAAACTG ACAATATATT CAGAGGCAGA TCTAAGAAAG 
AAAATGGTAG AAGAAGAACA GAAAAACCAT TTATCTGGTG AAATATGTGA AATGCAGACC 
GAAGAATTAG CTGGAAATTC TGAGACACTA AAAGAACCTG AAACGGTGGG AGCCCAGAGT 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868989.1
CDS	496..6381
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013500.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013500.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GAAACTCCCG TTATTGAAAA TCGTAGTTCA CAACAACCTG TAAGTGAAGT TTCTACCATC 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CCTCGCAGCT TCTAACTTCA 
GTAAGGCGCA TGGTTTTGAC CCTTACCCAG CAAAATGATG AGAGCAAAGA GTTTGTAAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTCAATGAA 
TTGGCTTTCT TTAAGCTTAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAGA 
TGCAAAAGGA AAATAGAAGC AACTGGAGTG ATACAATCTT GTGCCAAAGA GGCTAAAAGG 
ATTCTTGAAG ATCATGGCTC ACCTGCTGGA GAGATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCA GACTCAAACA TCTGAGGTGT ATGATGGTCC CAAAAATGTA 
AGATCTGATA TTTCTGATCA AGAGGAAGAT GAAGAAAGTG AAGGATGTCC AGTGTCTATT 
AATTTGTCTA AAGCTGAAAC TCAGGCTTTA ACTAATTATG GAAGTGGAGA AGATGAAAAT 
GAGGATGAAG AAATGGAAGA ATTTGAAGAA GGCCCTGTGG ATGTCCAGAC TTCCCTCCAG 
GCTAACACTG AAGCTACTGA AGAAAATGAA CATGATGAAC AGGTCCTACA ACGTGACTTT 
AAAAAGACAG CAGAAAGCAA AAATGTCCCA TTGGAACGAG AAGCCACTAG TAAAAATGAC 
CAAAATAACT GTCCTGTGAA ACCCTGTTAC CTCAATATCT TGGAAGATGA GCAACCTTTA 
AATAGTGCTG CCCATAAGGA GTCACCTCCT ACTGTTGATT CAACTCAACA GCCTAACCCT 
TTGCCGTTAC GTTTACCTGA AATGGAACCC TTAGTGCCTA GAGTCAAAGA AGTTAAATCT 
GCTCAGGAAA CTCCTGAAAG CTCTCTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA 
GTGAATGACT ATGAAGCAGA ATCTGGTAAT ATAAGTCAAA AGTCTGATGA AGAAGATTTT 
GTAAAAGTTG AAGATTTACC ACTGAAACTG ACAATATATT CAGAGGCAGA TCTAAGAAAG 
AAAATGGTAG AAGAAGAACA GAAAAACCAT TTATCTGGTG AAATATGTGA AATGCAGACC 
GAAGAATTAG CTGGAAATTC TGAGACACTA AAAGAACCTG AAACGGTGGG AGCCCAGAGT 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013503.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868992.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X43, mRNA.

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CloneID	OHu98036
Clone ID Related Accession (Same CDS sequence)	XM_017013503.1 , NM_001352643.2
Accession Version	XM_017013503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X30
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGTA TTCATCCAAG AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868992.1
CDS	496..5043
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDVFIQEK

Target ORF information:

RefSeq Version	XM_017013503.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X43, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013503.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGTA TTCATCCAAG AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352640.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339569.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 12, mRNA.

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CloneID	OHu42081
Clone ID Related Accession (Same CDS sequence)	NM_001352640.2
Accession Version	NM_001352640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352640.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.130124.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGGTACTA CAGTTAATAA TTTAGAAGAA ACTCCCGTTA TTGAAAATCG TAGTTCACAA 
CAACCTGTAA GTGAAGTTTC TACCATCCCA TGTCCTAGAA TTGATACTCA GCAGCTGGAC 
CGGCAAATTA AAGCAATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGAGCA CATGGATGAA 
GTATGCTCCT CGCAGCTTCT AACTTCAGTA AGGCGCATGG TTTTGACCCT TACCCAGCAA 
AATGATGAGA GCAAAGAGTT TGTAAAGTTC TTTCATAAAC AACTTGGAAG TATATTACAG 
GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAAGACT GTGGAGAAGA TCTTCTTGTA 
GAGATATCTG AAGTGTTGTT CAATGAATTG GCTTTCTTTA AGCTTATGCA AGATTTGGAT 
AATAATAGTA TAACTGTTAA ACAGAGATGC AAAAGGAAAA TAGAAGCAAC TGGAGTGATA 
CAATCTTGTG CCAAAGAGGC TAAAAGGATT CTTGAAGATC ATGGCTCACC TGCTGGAGAG 
ATTGATGATG AAGACAAAGA CAAGGATGAA ACTGAAACAG TTAAGCAGAC TCAAACATCT 
GAGGTGTATG ATGGTCCCAA AAATGTAAGA TCTGATATTT CTGATCAAGA GGAAGATGAA 
GAAAGTGAAG GATGTCCAGT GTCTATTAAT TTGTCTAAAG CTGAAACTCA GGCTTTAACT 
AATTATGGAA GTGGAGAAGA TGAAAATGAG GATGAAGAAA TGGAAGAATT TGAAGAAGGC 
CCTGTGGATG TCCAGACTTC CCTCCAGGCT AACACTGAAG CTACTGAAGA AAATGAACAT 
GATGAACAGG TCCTACAACG TGACTTTAAA AAGACAGCAG AAAGCAAAAA TGTCCCATTG 
GAACGAGAAG CCACTAGTAA AAATGACCAA AATAACTGTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAGATGAGCA ACCTTTAAAT AGTGCTGCCC ATAAGGAGTC ACCTCCTACT 
GTTGATTCAA CTCAACAGCC TAACCCTTTG CCGTTACGTT TACCTGAAAT GGAACCCTTA 
GTGCCTAGAG TCAAAGAAGT TAAATCTGCT CAGGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAATATA 
AGTCAAAAGT CTGATGAAGA AGATTTTGTA AAAGTTGAAG ATTTACCACT GAAACTGACA 
ATATATTCAG AGGCAGATCT AAGAAAGAAA ATGGTAGAAG AAGAACAGAA AAACCATTTA 
TCTGGTGAAA TATGTGAAAT GCAGACCGAA GAATTAGCTG GAAATTCTGA GACACTAAAA 
GAACCTGAAA CGGTGGGAGC CCAGAGTATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339569.2
CDS	292..6204
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352640.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352640.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGGTACTA CAGTTAATAA TTTAGAAGAA ACTCCCGTTA TTGAAAATCG TAGTTCACAA 
CAACCTGTAA GTGAAGTTTC TACCATCCCA TGTCCTAGAA TTGATACTCA GCAGCTGGAC 
CGGCAAATTA AAGCAATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGAGCA CATGGATGAA 
GTATGCTCCT CGCAGCTTCT AACTTCAGTA AGGCGCATGG TTTTGACCCT TACCCAGCAA 
AATGATGAGA GCAAAGAGTT TGTAAAGTTC TTTCATAAAC AACTTGGAAG TATATTACAG 
GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAAGACT GTGGAGAAGA TCTTCTTGTA 
GAGATATCTG AAGTGTTGTT CAATGAATTG GCTTTCTTTA AGCTTATGCA AGATTTGGAT 
AATAATAGTA TAACTGTTAA ACAGAGATGC AAAAGGAAAA TAGAAGCAAC TGGAGTGATA 
CAATCTTGTG CCAAAGAGGC TAAAAGGATT CTTGAAGATC ATGGCTCACC TGCTGGAGAG 
ATTGATGATG AAGACAAAGA CAAGGATGAA ACTGAAACAG TTAAGCAGAC TCAAACATCT 
GAGGTGTATG ATGGTCCCAA AAATGTAAGA TCTGATATTT CTGATCAAGA GGAAGATGAA 
GAAAGTGAAG GATGTCCAGT GTCTATTAAT TTGTCTAAAG CTGAAACTCA GGCTTTAACT 
AATTATGGAA GTGGAGAAGA TGAAAATGAG GATGAAGAAA TGGAAGAATT TGAAGAAGGC 
CCTGTGGATG TCCAGACTTC CCTCCAGGCT AACACTGAAG CTACTGAAGA AAATGAACAT 
GATGAACAGG TCCTACAACG TGACTTTAAA AAGACAGCAG AAAGCAAAAA TGTCCCATTG 
GAACGAGAAG CCACTAGTAA AAATGACCAA AATAACTGTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAGATGAGCA ACCTTTAAAT AGTGCTGCCC ATAAGGAGTC ACCTCCTACT 
GTTGATTCAA CTCAACAGCC TAACCCTTTG CCGTTACGTT TACCTGAAAT GGAACCCTTA 
GTGCCTAGAG TCAAAGAAGT TAAATCTGCT CAGGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAATATA 
AGTCAAAAGT CTGATGAAGA AGATTTTGTA AAAGTTGAAG ATTTACCACT GAAACTGACA 
ATATATTCAG AGGCAGATCT AAGAAAGAAA ATGGTAGAAG AAGAACAGAA AAACCATTTA 
TCTGGTGAAA TATGTGAAAT GCAGACCGAA GAATTAGCTG GAAATTCTGA GACACTAAAA 
GAACCTGAAA CGGTGGGAGC CCAGAGTATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001315507.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001302436.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 2, mRNA.

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CloneID	OHu100520
Clone ID Related Accession (Same CDS sequence)	NM_001315507.1
Accession Version	NM_001315507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC140946.1, AC087625.12 and AW237120.1. On Oct 8, 2015 this sequence version replaced XM_005273521.3. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.100904.1, BC140946.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGAAAC TCCCGTTATT 
GAAAATCGTA GTTCACAACA ACCTGTAAGT GAAGTTTCTA CCATCCCATG TCCTAGAATT 
GATACTCAGC AGCTGGACCG GCAAATTAAA GCAATTATGA AAGAAGTCAT TCCTTTTTTG 
AAGGAGCACA TGGATGAAGT ATGCTCCTCG CAGCTTCTAA CTTCAGTAAG GCGCATGGTT 
TTGACCCTTA CCCAGCAAAA TGATGAGAGC AAAGAGTTTG TAAAGTTCTT TCATAAACAA 
CTTGGAAGTA TATTACAGGA TTCACTGGCA AAATTTGCTG GCAGAAAACT GAAAGACTGT 
GGAGAAGATC TTCTTGTAGA GATATCTGAA GTGTTGTTCA ATGAATTGGC TTTCTTTAAG 
CTTATGCAAG ATTTGGATAA TAATAGTATA ACTGTTAAAC AGAGATGCAA AAGGAAAATA 
GAAGCAACTG GAGTGATACA ATCTTGTGCC AAAGAGGCTA AAAGGATTCT TGAAGATCAT 
GGCTCACCTG CTGGAGAGAT TGATGATGAA GACAAAGACA AGGATGAAAC TGAAACAGTT 
AAGCAGACTC AAACATCTGA GGTGTATGAT GGTCCCAAAA ATGTAAGATC TGATATTTCT 
GATCAAGAGG AAGATGAAGA AAGTGAAGGA TGTCCAGTGT CTATTAATTT GTCTAAAGCT 
GAAACTCAGG CTTTAACTAA TTATGGAAGT GGAGAAGATG AAAATGAGGA TGAAGAAATG 
GAAGAATTTG AAGAAGGCCC TGTGGATGTC CAGACTTCCC TCCAGGCTAA CACTGAAGCT 
ACTGAAGAAA ATGAACATGA TGAACAGGTC CTACAACGTG ACTTTAAAAA GACAGCAGAA 
AGCAAAAATG TCCCATTGGA ACGAGAAGCC ACTAGTAAAA ATGACCAAAA TAACTGTCCT 
GTGAAACCCT GTTACCTCAA TATCTTGGAA GATGAGCAAC CTTTAAATAG TGCTGCCCAT 
AAGGAGTCAC CTCCTACTGT TGATTCAACT CAACAGCCTA ACCCTTTGCC GTTACGTTTA 
CCTGAAATGG AACCCTTAGT GCCTAGAGTC AAAGAAGTTA AATCTGCTCA GGAAACTCCT 
GAAAGCTCTC TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA 
GCAGAATCTG GTAATATAAG TCAAAAGTCT GATGAAGAAG ATTTTGTAAA AGTTGAAGAT 
TTACCACTGA AACTGACAAT ATATTCAGAG GCAGATCTAA GAAAGAAAAT GGTAGAAGAA 
GAACAGAAAA ACCATTTATC TGGTGAAATA TGTGAAATGC AGACCGAAGA ATTAGCTGGA 
AATTCTGAGA CACTAAAAGA ACCTGAAACG GTGGGAGCCC AGAGTATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001302436.1
CDS	423..6473
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001315507.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001315507.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGAAAC TCCCGTTATT 
GAAAATCGTA GTTCACAACA ACCTGTAAGT GAAGTTTCTA CCATCCCATG TCCTAGAATT 
GATACTCAGC AGCTGGACCG GCAAATTAAA GCAATTATGA AAGAAGTCAT TCCTTTTTTG 
AAGGAGCACA TGGATGAAGT ATGCTCCTCG CAGCTTCTAA CTTCAGTAAG GCGCATGGTT 
TTGACCCTTA CCCAGCAAAA TGATGAGAGC AAAGAGTTTG TAAAGTTCTT TCATAAACAA 
CTTGGAAGTA TATTACAGGA TTCACTGGCA AAATTTGCTG GCAGAAAACT GAAAGACTGT 
GGAGAAGATC TTCTTGTAGA GATATCTGAA GTGTTGTTCA ATGAATTGGC TTTCTTTAAG 
CTTATGCAAG ATTTGGATAA TAATAGTATA ACTGTTAAAC AGAGATGCAA AAGGAAAATA 
GAAGCAACTG GAGTGATACA ATCTTGTGCC AAAGAGGCTA AAAGGATTCT TGAAGATCAT 
GGCTCACCTG CTGGAGAGAT TGATGATGAA GACAAAGACA AGGATGAAAC TGAAACAGTT 
AAGCAGACTC AAACATCTGA GGTGTATGAT GGTCCCAAAA ATGTAAGATC TGATATTTCT 
GATCAAGAGG AAGATGAAGA AAGTGAAGGA TGTCCAGTGT CTATTAATTT GTCTAAAGCT 
GAAACTCAGG CTTTAACTAA TTATGGAAGT GGAGAAGATG AAAATGAGGA TGAAGAAATG 
GAAGAATTTG AAGAAGGCCC TGTGGATGTC CAGACTTCCC TCCAGGCTAA CACTGAAGCT 
ACTGAAGAAA ATGAACATGA TGAACAGGTC CTACAACGTG ACTTTAAAAA GACAGCAGAA 
AGCAAAAATG TCCCATTGGA ACGAGAAGCC ACTAGTAAAA ATGACCAAAA TAACTGTCCT 
GTGAAACCCT GTTACCTCAA TATCTTGGAA GATGAGCAAC CTTTAAATAG TGCTGCCCAT 
AAGGAGTCAC CTCCTACTGT TGATTCAACT CAACAGCCTA ACCCTTTGCC GTTACGTTTA 
CCTGAAATGG AACCCTTAGT GCCTAGAGTC AAAGAAGTTA AATCTGCTCA GGAAACTCCT 
GAAAGCTCTC TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA 
GCAGAATCTG GTAATATAAG TCAAAAGTCT GATGAAGAAG ATTTTGTAAA AGTTGAAGAT 
TTACCACTGA AACTGACAAT ATATTCAGAG GCAGATCTAA GAAAGAAAAT GGTAGAAGAA 
GAACAGAAAA ACCATTTATC TGGTGAAATA TGTGAAATGC AGACCGAAGA ATTAGCTGGA 
AATTCTGAGA CACTAAAAGA ACCTGAAACG GTGGGAGCCC AGAGTATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352650.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339579.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 22, mRNA.

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CloneID	OHu103165
Clone ID Related Accession (Same CDS sequence)	NM_001352650.1 , NM_001352651.1
Accession Version	NM_001352650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 19
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013475.1. Transcript Variant: This variant (22), as well as variant 23, encodes isoform 19. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.26289.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCCTC GCAGCTTCTA 
ACTTCAGTAA GGCGCATGGT TTTGACCCTT ACCCAGCAAA ATGATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTC 
AATGAATTGG CTTTCTTTAA GCTTATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCAACT GGAGTGATAC AATCTTGTGC CAAAGAGGCT 
AAAAGGATTC TTGAAGATCA TGGCTCACCT GCTGGAGAGA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCAGACT CAAACATCTG AGGTGTATGA TGGTCCCAAA 
AATGTAAGAT CTGATATTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGG ATGTCCAGTG 
TCTATTAATT TGTCTAAAGC TGAAACTCAG GCTTTAACTA ATTATGGAAG TGGAGAAGAT 
GAAAATGAGG ATGAAGAAAT GGAAGAATTT GAAGAAGGCC CTGTGGATGT CCAGACTTCC 
CTCCAGGCTA ACACTGAAGC TACTGAAGAA AATGAACATG ATGAACAGGT CCTACAACGT 
GACTTTAAAA AGACAGCAGA AAGCAAAAAT GTCCCATTGG AACGAGAAGC CACTAGTAAA 
AATGACCAAA ATAACTGTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AGATGAGCAA 
CCTTTAAATA GTGCTGCCCA TAAGGAGTCA CCTCCTACTG TTGATTCAAC TCAACAGCCT 
AACCCTTTGC CGTTACGTTT ACCTGAAATG GAACCCTTAG TGCCTAGAGT CAAAGAAGTT 
AAATCTGCTC AGGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAATATAA GTCAAAAGTC TGATGAAGAA 
GATTTTGTAA AAGTTGAAGA TTTACCACTG AAACTGACAA TATATTCAGA GGCAGATCTA 
AGAAAGAAAA TGGTAGAAGA AGAACAGAAA AACCATTTAT CTGGTGAAAT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG AAATTCTGAG ACACTAAAAG AACCTGAAAC GGTGGGAGCC 
CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339579.1
CDS	760..6951
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352650.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352650.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCCTC GCAGCTTCTA 
ACTTCAGTAA GGCGCATGGT TTTGACCCTT ACCCAGCAAA ATGATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTC 
AATGAATTGG CTTTCTTTAA GCTTATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCAACT GGAGTGATAC AATCTTGTGC CAAAGAGGCT 
AAAAGGATTC TTGAAGATCA TGGCTCACCT GCTGGAGAGA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCAGACT CAAACATCTG AGGTGTATGA TGGTCCCAAA 
AATGTAAGAT CTGATATTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGG ATGTCCAGTG 
TCTATTAATT TGTCTAAAGC TGAAACTCAG GCTTTAACTA ATTATGGAAG TGGAGAAGAT 
GAAAATGAGG ATGAAGAAAT GGAAGAATTT GAAGAAGGCC CTGTGGATGT CCAGACTTCC 
CTCCAGGCTA ACACTGAAGC TACTGAAGAA AATGAACATG ATGAACAGGT CCTACAACGT 
GACTTTAAAA AGACAGCAGA AAGCAAAAAT GTCCCATTGG AACGAGAAGC CACTAGTAAA 
AATGACCAAA ATAACTGTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AGATGAGCAA 
CCTTTAAATA GTGCTGCCCA TAAGGAGTCA CCTCCTACTG TTGATTCAAC TCAACAGCCT 
AACCCTTTGC CGTTACGTTT ACCTGAAATG GAACCCTTAG TGCCTAGAGT CAAAGAAGTT 
AAATCTGCTC AGGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAATATAA GTCAAAAGTC TGATGAAGAA 
GATTTTGTAA AAGTTGAAGA TTTACCACTG AAACTGACAA TATATTCAGA GGCAGATCTA 
AGAAAGAAAA TGGTAGAAGA AGAACAGAAA AACCATTTAT CTGGTGAAAT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG AAATTCTGAG ACACTAAAAG AACCTGAAAC GGTGGGAGCC 
CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352651.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339580.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 23, mRNA.

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CloneID	OHu103165
Clone ID Related Accession (Same CDS sequence)	NM_001352650.1 , NM_001352651.1
Accession Version	NM_001352651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 19
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013476.1. Transcript Variant: This variant (23), as well as variant 22, encodes isoform 19. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.211119.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCCTC GCAGCTTCTA 
ACTTCAGTAA GGCGCATGGT TTTGACCCTT ACCCAGCAAA ATGATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTC 
AATGAATTGG CTTTCTTTAA GCTTATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCAACT GGAGTGATAC AATCTTGTGC CAAAGAGGCT 
AAAAGGATTC TTGAAGATCA TGGCTCACCT GCTGGAGAGA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCAGACT CAAACATCTG AGGTGTATGA TGGTCCCAAA 
AATGTAAGAT CTGATATTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGG ATGTCCAGTG 
TCTATTAATT TGTCTAAAGC TGAAACTCAG GCTTTAACTA ATTATGGAAG TGGAGAAGAT 
GAAAATGAGG ATGAAGAAAT GGAAGAATTT GAAGAAGGCC CTGTGGATGT CCAGACTTCC 
CTCCAGGCTA ACACTGAAGC TACTGAAGAA AATGAACATG ATGAACAGGT CCTACAACGT 
GACTTTAAAA AGACAGCAGA AAGCAAAAAT GTCCCATTGG AACGAGAAGC CACTAGTAAA 
AATGACCAAA ATAACTGTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AGATGAGCAA 
CCTTTAAATA GTGCTGCCCA TAAGGAGTCA CCTCCTACTG TTGATTCAAC TCAACAGCCT 
AACCCTTTGC CGTTACGTTT ACCTGAAATG GAACCCTTAG TGCCTAGAGT CAAAGAAGTT 
AAATCTGCTC AGGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAATATAA GTCAAAAGTC TGATGAAGAA 
GATTTTGTAA AAGTTGAAGA TTTACCACTG AAACTGACAA TATATTCAGA GGCAGATCTA 
AGAAAGAAAA TGGTAGAAGA AGAACAGAAA AACCATTTAT CTGGTGAAAT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG AAATTCTGAG ACACTAAAAG AACCTGAAAC GGTGGGAGCC 
CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339580.1
CDS	355..6546
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352651.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352651.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCCTC GCAGCTTCTA 
ACTTCAGTAA GGCGCATGGT TTTGACCCTT ACCCAGCAAA ATGATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTC 
AATGAATTGG CTTTCTTTAA GCTTATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCAACT GGAGTGATAC AATCTTGTGC CAAAGAGGCT 
AAAAGGATTC TTGAAGATCA TGGCTCACCT GCTGGAGAGA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCAGACT CAAACATCTG AGGTGTATGA TGGTCCCAAA 
AATGTAAGAT CTGATATTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGG ATGTCCAGTG 
TCTATTAATT TGTCTAAAGC TGAAACTCAG GCTTTAACTA ATTATGGAAG TGGAGAAGAT 
GAAAATGAGG ATGAAGAAAT GGAAGAATTT GAAGAAGGCC CTGTGGATGT CCAGACTTCC 
CTCCAGGCTA ACACTGAAGC TACTGAAGAA AATGAACATG ATGAACAGGT CCTACAACGT 
GACTTTAAAA AGACAGCAGA AAGCAAAAAT GTCCCATTGG AACGAGAAGC CACTAGTAAA 
AATGACCAAA ATAACTGTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AGATGAGCAA 
CCTTTAAATA GTGCTGCCCA TAAGGAGTCA CCTCCTACTG TTGATTCAAC TCAACAGCCT 
AACCCTTTGC CGTTACGTTT ACCTGAAATG GAACCCTTAG TGCCTAGAGT CAAAGAAGTT 
AAATCTGCTC AGGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAATATAA GTCAAAAGTC TGATGAAGAA 
GATTTTGTAA AAGTTGAAGA TTTACCACTG AAACTGACAA TATATTCAGA GGCAGATCTA 
AGAAAGAAAA TGGTAGAAGA AGAACAGAAA AACCATTTAT CTGGTGAAAT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG AAATTCTGAG ACACTAAAAG AACCTGAAAC GGTGGGAGCC 
CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352633.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339562.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 5, mRNA.

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CloneID	OHu103173
Clone ID Related Accession (Same CDS sequence)	NM_001352633.1
Accession Version	NM_001352633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013477.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.36010.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA TTGTCACTTT TTTTACTCAA 
GGGTATGAAA GTGCCAGTAT GTCTAGCACA TGTGAACCTT GCAAAAGTAG GAACAGACAT 
TCAGCCCAGA CTGAAGAGCC TGTTCAAGCA AAAGTATTCA GCAGAAAGAA TCATGAGCAA 
CTGGAAAAAA TAATAAAATG TAATAGGTCT ACAGAAATAT CTTCAGAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339562.1
CDS	760..6948
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIIVTFFTQGYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352633.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352633.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA TTGTCACTTT TTTTACTCAA 
GGGTATGAAA GTGCCAGTAT GTCTAGCACA TGTGAACCTT GCAAAAGTAG GAACAGACAT 
TCAGCCCAGA CTGAAGAGCC TGTTCAAGCA AAAGTATTCA GCAGAAAGAA TCATGAGCAA 
CTGGAAAAAA TAATAAAATG TAATAGGTCT ACAGAAATAT CTTCAGAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352634.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339563.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 6, mRNA.

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CloneID	OHu103176
Clone ID Related Accession (Same CDS sequence)	NM_001352634.1
Accession Version	NM_001352634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013482.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.198445.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAT TTTTCTCAGG AGCACATGGA TGAAGTATGC 
TCCTCGCAGC TTCTAACTTC AGTAAGGCGC ATGGTTTTGA CCCTTACCCA GCAAAATGAT 
GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT AAACAACTTG GAAGTATATT ACAGGATTCA 
CTGGCAAAAT TTGCTGGCAG AAAACTGAAA GACTGTGGAG AAGATCTTCT TGTAGAGATA 
TCTGAAGTGT TGTTCAATGA ATTGGCTTTC TTTAAGCTTA TGCAAGATTT GGATAATAAT 
AGTATAACTG TTAAACAGAG ATGCAAAAGG AAAATAGAAG CAACTGGAGT GATACAATCT 
TGTGCCAAAG AGGCTAAAAG GATTCTTGAA GATCATGGCT CACCTGCTGG AGAGATTGAT 
GATGAAGACA AAGACAAGGA TGAAACTGAA ACAGTTAAGC AGACTCAAAC ATCTGAGGTG 
TATGATGGTC CCAAAAATGT AAGATCTGAT ATTTCTGATC AAGAGGAAGA TGAAGAAAGT 
GAAGGATGTC CAGTGTCTAT TAATTTGTCT AAAGCTGAAA CTCAGGCTTT AACTAATTAT 
GGAAGTGGAG AAGATGAAAA TGAGGATGAA GAAATGGAAG AATTTGAAGA AGGCCCTGTG 
GATGTCCAGA CTTCCCTCCA GGCTAACACT GAAGCTACTG AAGAAAATGA ACATGATGAA 
CAGGTCCTAC AACGTGACTT TAAAAAGACA GCAGAAAGCA AAAATGTCCC ATTGGAACGA 
GAAGCCACTA GTAAAAATGA CCAAAATAAC TGTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAGATG AGCAACCTTT AAATAGTGCT GCCCATAAGG AGTCACCTCC TACTGTTGAT 
TCAACTCAAC AGCCTAACCC TTTGCCGTTA CGTTTACCTG AAATGGAACC CTTAGTGCCT 
AGAGTCAAAG AAGTTAAATC TGCTCAGGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA TATAAGTCAA 
AAGTCTGATG AAGAAGATTT TGTAAAAGTT GAAGATTTAC CACTGAAACT GACAATATAT 
TCAGAGGCAG ATCTAAGAAA GAAAATGGTA GAAGAAGAAC AGAAAAACCA TTTATCTGGT 
GAAATATGTG AAATGCAGAC CGAAGAATTA GCTGGAAATT CTGAGACACT AAAAGAACCT 
GAAACGGTGG GAGCCCAGAG TATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339563.1
CDS	423..6509
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352634.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352634.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAT TTTTCTCAGG AGCACATGGA TGAAGTATGC 
TCCTCGCAGC TTCTAACTTC AGTAAGGCGC ATGGTTTTGA CCCTTACCCA GCAAAATGAT 
GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT AAACAACTTG GAAGTATATT ACAGGATTCA 
CTGGCAAAAT TTGCTGGCAG AAAACTGAAA GACTGTGGAG AAGATCTTCT TGTAGAGATA 
TCTGAAGTGT TGTTCAATGA ATTGGCTTTC TTTAAGCTTA TGCAAGATTT GGATAATAAT 
AGTATAACTG TTAAACAGAG ATGCAAAAGG AAAATAGAAG CAACTGGAGT GATACAATCT 
TGTGCCAAAG AGGCTAAAAG GATTCTTGAA GATCATGGCT CACCTGCTGG AGAGATTGAT 
GATGAAGACA AAGACAAGGA TGAAACTGAA ACAGTTAAGC AGACTCAAAC ATCTGAGGTG 
TATGATGGTC CCAAAAATGT AAGATCTGAT ATTTCTGATC AAGAGGAAGA TGAAGAAAGT 
GAAGGATGTC CAGTGTCTAT TAATTTGTCT AAAGCTGAAA CTCAGGCTTT AACTAATTAT 
GGAAGTGGAG AAGATGAAAA TGAGGATGAA GAAATGGAAG AATTTGAAGA AGGCCCTGTG 
GATGTCCAGA CTTCCCTCCA GGCTAACACT GAAGCTACTG AAGAAAATGA ACATGATGAA 
CAGGTCCTAC AACGTGACTT TAAAAAGACA GCAGAAAGCA AAAATGTCCC ATTGGAACGA 
GAAGCCACTA GTAAAAATGA CCAAAATAAC TGTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAGATG AGCAACCTTT AAATAGTGCT GCCCATAAGG AGTCACCTCC TACTGTTGAT 
TCAACTCAAC AGCCTAACCC TTTGCCGTTA CGTTTACCTG AAATGGAACC CTTAGTGCCT 
AGAGTCAAAG AAGTTAAATC TGCTCAGGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA TATAAGTCAA 
AAGTCTGATG AAGAAGATTT TGTAAAAGTT GAAGATTTAC CACTGAAACT GACAATATAT 
TCAGAGGCAG ATCTAAGAAA GAAAATGGTA GAAGAAGAAC AGAAAAACCA TTTATCTGGT 
GAAATATGTG AAATGCAGAC CGAAGAATTA GCTGGAAATT CTGAGACACT AAAAGAACCT 
GAAACGGTGG GAGCCCAGAG TATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352638.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339567.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 10, mRNA.

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CloneID	OHu103178
Clone ID Related Accession (Same CDS sequence)	NM_001352638.1
Accession Version	NM_001352638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.55097.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149178, SAMN02400288 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGAA 
ACTCCCGTTA TTGAAAATCG TAGTTCACAA CAACCTGTAA GTGAAGTTTC TACCATCCCA 
TGTCCTAGAA TTGATACTCA GCAGCTGGAC CGGCAAATTA AAGCAATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGCTCCT CGCAGCTTCT AACTTCAGTA 
AGGCGCATGG TTTTGACCCT TACCCAGCAA AATGATGAGA GCAAAGAGTT TGTAAAGTTC 
TTTCATAAAC AACTTGGAAG TATATTACAG GATTCACTGG CAAAATTTGC TGGCAGAAAA 
CTGAAAGACT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTGTT CAATGAATTG 
GCTTTCTTTA AGCTTATGCA AGATTTGGAT AATAATAGTA TAACTGTTAA ACAGAGATGC 
AAAAGGAAAA TAGAAGCAAC TGGAGTGATA CAATCTTGTG CCAAAGAGGC TAAAAGGATT 
CTTGAAGATC ATGGCTCACC TGCTGGAGAG ATTGATGATG AAGACAAAGA CAAGGATGAA 
ACTGAAACAG TTAAGCAGAC TCAAACATCT GAGGTGTATG ATGGTCCCAA AAATGTAAGA 
TCTGATATTT CTGATCAAGA GGAAGATGAA GAAAGTGAAG GATGTCCAGT GTCTATTAAT 
TTGTCTAAAG CTGAAACTCA GGCTTTAACT AATTATGGAA GTGGAGAAGA TGAAAATGAG 
GATGAAGAAA TGGAAGAATT TGAAGAAGGC CCTGTGGATG TCCAGACTTC CCTCCAGGCT 
AACACTGAAG CTACTGAAGA AAATGAACAT GATGAACAGG TCCTACAACG TGACTTTAAA 
AAGACAGCAG AAAGCAAAAA TGTCCCATTG GAACGAGAAG CCACTAGTAA AAATGACCAA 
AATAACTGTC CTGTGAAACC CTGTTACCTC AATATCTTGG AAGATGAGCA ACCTTTAAAT 
AGTGCTGCCC ATAAGGAGTC ACCTCCTACT GTTGATTCAA CTCAACAGCC TAACCCTTTG 
CCGTTACGTT TACCTGAAAT GGAACCCTTA GTGCCTAGAG TCAAAGAAGT TAAATCTGCT 
CAGGAAACTC CTGAAAGCTC TCTGGCTGGA AGTCCTGATA CTGAATCTCC AGTGTTAGTG 
AATGACTATG AAGCAGAATC TGGTAATATA AGTCAAAAGT CTGATGAAGA AGATTTTGTA 
AAAGTTGAAG ATTTACCACT GAAACTGACA ATATATTCAG AGGCAGATCT AAGAAAGAAA 
ATGGTAGAAG AAGAACAGAA AAACCATTTA TCTGGTGAAA TATGTGAAAT GCAGACCGAA 
GAATTAGCTG GAAATTCTGA GACACTAAAA GAACCTGAAA CGGTGGGAGC CCAGAGTATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339567.1
CDS	692..6694
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352638.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352638.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGAA 
ACTCCCGTTA TTGAAAATCG TAGTTCACAA CAACCTGTAA GTGAAGTTTC TACCATCCCA 
TGTCCTAGAA TTGATACTCA GCAGCTGGAC CGGCAAATTA AAGCAATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGCTCCT CGCAGCTTCT AACTTCAGTA 
AGGCGCATGG TTTTGACCCT TACCCAGCAA AATGATGAGA GCAAAGAGTT TGTAAAGTTC 
TTTCATAAAC AACTTGGAAG TATATTACAG GATTCACTGG CAAAATTTGC TGGCAGAAAA 
CTGAAAGACT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTGTT CAATGAATTG 
GCTTTCTTTA AGCTTATGCA AGATTTGGAT AATAATAGTA TAACTGTTAA ACAGAGATGC 
AAAAGGAAAA TAGAAGCAAC TGGAGTGATA CAATCTTGTG CCAAAGAGGC TAAAAGGATT 
CTTGAAGATC ATGGCTCACC TGCTGGAGAG ATTGATGATG AAGACAAAGA CAAGGATGAA 
ACTGAAACAG TTAAGCAGAC TCAAACATCT GAGGTGTATG ATGGTCCCAA AAATGTAAGA 
TCTGATATTT CTGATCAAGA GGAAGATGAA GAAAGTGAAG GATGTCCAGT GTCTATTAAT 
TTGTCTAAAG CTGAAACTCA GGCTTTAACT AATTATGGAA GTGGAGAAGA TGAAAATGAG 
GATGAAGAAA TGGAAGAATT TGAAGAAGGC CCTGTGGATG TCCAGACTTC CCTCCAGGCT 
AACACTGAAG CTACTGAAGA AAATGAACAT GATGAACAGG TCCTACAACG TGACTTTAAA 
AAGACAGCAG AAAGCAAAAA TGTCCCATTG GAACGAGAAG CCACTAGTAA AAATGACCAA 
AATAACTGTC CTGTGAAACC CTGTTACCTC AATATCTTGG AAGATGAGCA ACCTTTAAAT 
AGTGCTGCCC ATAAGGAGTC ACCTCCTACT GTTGATTCAA CTCAACAGCC TAACCCTTTG 
CCGTTACGTT TACCTGAAAT GGAACCCTTA GTGCCTAGAG TCAAAGAAGT TAAATCTGCT 
CAGGAAACTC CTGAAAGCTC TCTGGCTGGA AGTCCTGATA CTGAATCTCC AGTGTTAGTG 
AATGACTATG AAGCAGAATC TGGTAATATA AGTCAAAAGT CTGATGAAGA AGATTTTGTA 
AAAGTTGAAG ATTTACCACT GAAACTGACA ATATATTCAG AGGCAGATCT AAGAAAGAAA 
ATGGTAGAAG AAGAACAGAA AAACCATTTA TCTGGTGAAA TATGTGAAAT GCAGACCGAA 
GAATTAGCTG GAAATTCTGA GACACTAAAA GAACCTGAAA CGGTGGGAGC CCAGAGTATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352632.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339561.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 4, mRNA.

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CloneID	OHu103195
Clone ID Related Accession (Same CDS sequence)	NM_001352632.1
Accession Version	NM_001352632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013474.1. Transcript Variant: This variant (4) encodes the longest isoform (4). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.246957.1, SRR1803612.19531.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGATTTT TCTCAGGAGC ACATGGATGA AGTATGCTCC 
TCGCAGCTTC TAACTTCAGT AAGGCGCATG GTTTTGACCC TTACCCAGCA AAATGATGAG 
AGCAAAGAGT TTGTAAAGTT CTTTCATAAA CAACTTGGAA GTATATTACA GGATTCACTG 
GCAAAATTTG CTGGCAGAAA ACTGAAAGAC TGTGGAGAAG ATCTTCTTGT AGAGATATCT 
GAAGTGTTGT TCAATGAATT GGCTTTCTTT AAGCTTATGC AAGATTTGGA TAATAATAGT 
ATAACTGTTA AACAGAGATG CAAAAGGAAA ATAGAAGCAA CTGGAGTGAT ACAATCTTGT 
GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT CATGGCTCAC CTGCTGGAGA GATTGATGAT 
GAAGACAAAG ACAAGGATGA AACTGAAACA GTTAAGCAGA CTCAAACATC TGAGGTGTAT 
GATGGTCCCA AAAATGTAAG ATCTGATATT TCTGATCAAG AGGAAGATGA AGAAAGTGAA 
GGATGTCCAG TGTCTATTAA TTTGTCTAAA GCTGAAACTC AGGCTTTAAC TAATTATGGA 
AGTGGAGAAG ATGAAAATGA GGATGAAGAA ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT 
GTCCAGACTT CCCTCCAGGC TAACACTGAA GCTACTGAAG AAAATGAACA TGATGAACAG 
GTCCTACAAC GTGACTTTAA AAAGACAGCA GAAAGCAAAA ATGTCCCATT GGAACGAGAA 
GCCACTAGTA AAAATGACCA AAATAACTGT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAGATGAGC AACCTTTAAA TAGTGCTGCC CATAAGGAGT CACCTCCTAC TGTTGATTCA 
ACTCAACAGC CTAACCCTTT GCCGTTACGT TTACCTGAAA TGGAACCCTT AGTGCCTAGA 
GTCAAAGAAG TTAAATCTGC TCAGGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAATAT AAGTCAAAAG 
TCTGATGAAG AAGATTTTGT AAAAGTTGAA GATTTACCAC TGAAACTGAC AATATATTCA 
GAGGCAGATC TAAGAAAGAA AATGGTAGAA GAAGAACAGA AAAACCATTT ATCTGGTGAA 
ATATGTGAAA TGCAGACCGA AGAATTAGCT GGAAATTCTG AGACACTAAA AGAACCTGAA 
ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339561.1
CDS	423..6626
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352632.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352632.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGATTTT TCTCAGGAGC ACATGGATGA AGTATGCTCC 
TCGCAGCTTC TAACTTCAGT AAGGCGCATG GTTTTGACCC TTACCCAGCA AAATGATGAG 
AGCAAAGAGT TTGTAAAGTT CTTTCATAAA CAACTTGGAA GTATATTACA GGATTCACTG 
GCAAAATTTG CTGGCAGAAA ACTGAAAGAC TGTGGAGAAG ATCTTCTTGT AGAGATATCT 
GAAGTGTTGT TCAATGAATT GGCTTTCTTT AAGCTTATGC AAGATTTGGA TAATAATAGT 
ATAACTGTTA AACAGAGATG CAAAAGGAAA ATAGAAGCAA CTGGAGTGAT ACAATCTTGT 
GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT CATGGCTCAC CTGCTGGAGA GATTGATGAT 
GAAGACAAAG ACAAGGATGA AACTGAAACA GTTAAGCAGA CTCAAACATC TGAGGTGTAT 
GATGGTCCCA AAAATGTAAG ATCTGATATT TCTGATCAAG AGGAAGATGA AGAAAGTGAA 
GGATGTCCAG TGTCTATTAA TTTGTCTAAA GCTGAAACTC AGGCTTTAAC TAATTATGGA 
AGTGGAGAAG ATGAAAATGA GGATGAAGAA ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT 
GTCCAGACTT CCCTCCAGGC TAACACTGAA GCTACTGAAG AAAATGAACA TGATGAACAG 
GTCCTACAAC GTGACTTTAA AAAGACAGCA GAAAGCAAAA ATGTCCCATT GGAACGAGAA 
GCCACTAGTA AAAATGACCA AAATAACTGT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAGATGAGC AACCTTTAAA TAGTGCTGCC CATAAGGAGT CACCTCCTAC TGTTGATTCA 
ACTCAACAGC CTAACCCTTT GCCGTTACGT TTACCTGAAA TGGAACCCTT AGTGCCTAGA 
GTCAAAGAAG TTAAATCTGC TCAGGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAATAT AAGTCAAAAG 
TCTGATGAAG AAGATTTTGT AAAAGTTGAA GATTTACCAC TGAAACTGAC AATATATTCA 
GAGGCAGATC TAAGAAAGAA AATGGTAGAA GAAGAACAGA AAAACCATTT ATCTGGTGAA 
ATATGTGAAA TGCAGACCGA AGAATTAGCT GGAAATTCTG AGACACTAAA AGAACCTGAA 
ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352635.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339564.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 7, mRNA.

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CloneID	OHu103196
Clone ID Related Accession (Same CDS sequence)	NM_001352635.1
Accession Version	NM_001352635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013485.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.266811.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAGGTA TGAAAGTGCC 
AGTATGTCTA GCACATGTGA ACCTTGCAAA AGTAGGAACA GACATTCAGC CCAGACTGAA 
GAGCCTGTTC AAGCAAAAGT ATTCAGCAGA AAGAATCATG AGCAACTGGA AAAAATAATA 
AAATGTAATA GGTCTACAGA AATATCTTCA GAAACTGGGA GTGATTTTTC CATGTTTGAA 
GCTTTGCGAG ATACTATTTA TTCTGAAGTA GCTACATTAA TTTCTCAAAA TGAATCTCGT 
CCACATTTTC TTATTGAACT CTTCCATGAG CTGCAGCTAC TAAACACAGA CTACTTGAGA 
CAGAGGGCTT TATATGCATT GCAGGACATA GTATCCAGAC ATATTTCTGA GAGCCATGAA 
AAAGGAGAAA ATGTAAAGTC AGTAAACTCT GGTACTTGGA TAGCATCAAA CTCAGAACTT 
ACTCCTAGTG AGAGCCTTGC TACTACTGAT GATGAAACTT TTGAGAAGAA CTTTGAAAGA 
GAAACCCATA AAATAAGTGA GCAAAATGAT GCTGATAATG CTAGTGTCCT GTCTGTATCA 
TCAAATTTTG AGCCTTTTGC AACAGATGAT CTAGGTAACA CCGTGATTCA CTTAGATCAA 
GCATTAGCCA GAATGAGAGA ATATGAGCGT ATGAAGACTG AGGCTGAAAG TAACTCAAAT 
ATGAGATGCA CCTGCAGGAT TATTGAGGAT GGAGATGGTG CTGGTGCAGA AACTCCCGTT 
ATTGAAAATC GTAGTTCACA ACAACCTGTA AGTGAAGTTT CTACCATCCC ATGTCCTAGA 
ATTGATACTC AGCAGCTGGA CCGGCAAATT AAAGCAATTA TGAAAGAAGT CATTCCTTTT 
TTGAAGGAGC ACATGGATGA AGTATGCTCC TCGCAGCTTC TAACTTCAGT AAGGCGCATG 
GTTTTGACCC TTACCCAGCA AAATGATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TCAATGAATT GGCTTTCTTT 
AAGCTTATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCAA CTGGAGTGAT ACAATCTTGT GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT 
CATGGCTCAC CTGCTGGAGA GATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCAGA CTCAAACATC TGAGGTGTAT GATGGTCCCA AAAATGTAAG ATCTGATATT 
TCTGATCAAG AGGAAGATGA AGAAAGTGAA GGATGTCCAG TGTCTATTAA TTTGTCTAAA 
GCTGAAACTC AGGCTTTAAC TAATTATGGA AGTGGAGAAG ATGAAAATGA GGATGAAGAA 
ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT GTCCAGACTT CCCTCCAGGC TAACACTGAA 
GCTACTGAAG AAAATGAACA TGATGAACAG GTCCTACAAC GTGACTTTAA AAAGACAGCA 
GAAAGCAAAA ATGTCCCATT GGAACGAGAA GCCACTAGTA AAAATGACCA AAATAACTGT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAGATGAGC AACCTTTAAA TAGTGCTGCC 
CATAAGGAGT CACCTCCTAC TGTTGATTCA ACTCAACAGC CTAACCCTTT GCCGTTACGT 
TTACCTGAAA TGGAACCCTT AGTGCCTAGA GTCAAAGAAG TTAAATCTGC TCAGGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAATAT AAGTCAAAAG TCTGATGAAG AAGATTTTGT AAAAGTTGAA 
GATTTACCAC TGAAACTGAC AATATATTCA GAGGCAGATC TAAGAAAGAA AATGGTAGAA 
GAAGAACAGA AAAACCATTT ATCTGGTGAA ATATGTGAAA TGCAGACCGA AGAATTAGCT 
GGAAATTCTG AGACACTAAA AGAACCTGAA ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339564.1
CDS	760..6813
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352635.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352635.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAGGTA TGAAAGTGCC 
AGTATGTCTA GCACATGTGA ACCTTGCAAA AGTAGGAACA GACATTCAGC CCAGACTGAA 
GAGCCTGTTC AAGCAAAAGT ATTCAGCAGA AAGAATCATG AGCAACTGGA AAAAATAATA 
AAATGTAATA GGTCTACAGA AATATCTTCA GAAACTGGGA GTGATTTTTC CATGTTTGAA 
GCTTTGCGAG ATACTATTTA TTCTGAAGTA GCTACATTAA TTTCTCAAAA TGAATCTCGT 
CCACATTTTC TTATTGAACT CTTCCATGAG CTGCAGCTAC TAAACACAGA CTACTTGAGA 
CAGAGGGCTT TATATGCATT GCAGGACATA GTATCCAGAC ATATTTCTGA GAGCCATGAA 
AAAGGAGAAA ATGTAAAGTC AGTAAACTCT GGTACTTGGA TAGCATCAAA CTCAGAACTT 
ACTCCTAGTG AGAGCCTTGC TACTACTGAT GATGAAACTT TTGAGAAGAA CTTTGAAAGA 
GAAACCCATA AAATAAGTGA GCAAAATGAT GCTGATAATG CTAGTGTCCT GTCTGTATCA 
TCAAATTTTG AGCCTTTTGC AACAGATGAT CTAGGTAACA CCGTGATTCA CTTAGATCAA 
GCATTAGCCA GAATGAGAGA ATATGAGCGT ATGAAGACTG AGGCTGAAAG TAACTCAAAT 
ATGAGATGCA CCTGCAGGAT TATTGAGGAT GGAGATGGTG CTGGTGCAGA AACTCCCGTT 
ATTGAAAATC GTAGTTCACA ACAACCTGTA AGTGAAGTTT CTACCATCCC ATGTCCTAGA 
ATTGATACTC AGCAGCTGGA CCGGCAAATT AAAGCAATTA TGAAAGAAGT CATTCCTTTT 
TTGAAGGAGC ACATGGATGA AGTATGCTCC TCGCAGCTTC TAACTTCAGT AAGGCGCATG 
GTTTTGACCC TTACCCAGCA AAATGATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TCAATGAATT GGCTTTCTTT 
AAGCTTATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCAA CTGGAGTGAT ACAATCTTGT GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT 
CATGGCTCAC CTGCTGGAGA GATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCAGA CTCAAACATC TGAGGTGTAT GATGGTCCCA AAAATGTAAG ATCTGATATT 
TCTGATCAAG AGGAAGATGA AGAAAGTGAA GGATGTCCAG TGTCTATTAA TTTGTCTAAA 
GCTGAAACTC AGGCTTTAAC TAATTATGGA AGTGGAGAAG ATGAAAATGA GGATGAAGAA 
ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT GTCCAGACTT CCCTCCAGGC TAACACTGAA 
GCTACTGAAG AAAATGAACA TGATGAACAG GTCCTACAAC GTGACTTTAA AAAGACAGCA 
GAAAGCAAAA ATGTCCCATT GGAACGAGAA GCCACTAGTA AAAATGACCA AAATAACTGT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAGATGAGC AACCTTTAAA TAGTGCTGCC 
CATAAGGAGT CACCTCCTAC TGTTGATTCA ACTCAACAGC CTAACCCTTT GCCGTTACGT 
TTACCTGAAA TGGAACCCTT AGTGCCTAGA GTCAAAGAAG TTAAATCTGC TCAGGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAATAT AAGTCAAAAG TCTGATGAAG AAGATTTTGT AAAAGTTGAA 
GATTTACCAC TGAAACTGAC AATATATTCA GAGGCAGATC TAAGAAAGAA AATGGTAGAA 
GAAGAACAGA AAAACCATTT ATCTGGTGAA ATATGTGAAA TGCAGACCGA AGAATTAGCT 
GGAAATTCTG AGACACTAAA AGAACCTGAA ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352636.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339565.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 8, mRNA.

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CloneID	OHu103198
Clone ID Related Accession (Same CDS sequence)	NM_001352636.1
Accession Version	NM_001352636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013487.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.5959.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GCAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339565.1
CDS	423..6452
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352636.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352636.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GCAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001315507.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001302436.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 2, mRNA.

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CloneID	OHu42080
Clone ID Related Accession (Same CDS sequence)	NM_001315507.2
Accession Version	NM_001315507.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001315507.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.100904.1, BC140946.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGAAAC TCCCGTTATT 
GAAAATCGTA GTTCACAACA ACCTGTAAGT GAAGTTTCTA CCATCCCATG TCCTAGAATT 
GATACTCAGC AGCTGGACCG GCAAATTAAA GCAATTATGA AAGAAGTCAT TCCTTTTTTG 
AAGGAGCACA TGGATGAAGT ATGCTCCTCG CAGCTTCTAA CTTCAGTAAG GCGCATGGTT 
TTGACCCTTA CCCAGCAAAA TGATGAGAGC AAAGAGTTTG TAAAGTTCTT TCATAAACAA 
CTTGGAAGTA TATTACAGGA TTCACTGGCA AAATTTGCTG GCAGAAAACT GAAAGACTGT 
GGAGAAGATC TTCTTGTAGA GATATCTGAA GTGTTGTTCA ATGAATTGGC TTTCTTTAAG 
CTTATGCAAG ATTTGGATAA TAATAGTATA ACTGTTAAAC AGAGATGCAA AAGGAAAATA 
GAAGCAACTG GAGTGATACA ATCTTGTGCC AAAGAGGCTA AAAGGATTCT TGAAGATCAT 
GGCTCACCTG CTGGAGAGAT TGATGATGAA GACAAAGACA AGGATGAAAC TGAAACAGTT 
AAGCAGACTC AAACATCTGA GGTGTATGAT GGTCCCAAAA ATGTAAGATC TGATATTTCT 
GATCAAGAGG AAGATGAAGA AAGTGAAGGA TGTCCAGTGT CTATTAATTT GTCTAAAGCT 
GAAACTCAGG CTTTAACTAA TTATGGAAGT GGAGAAGATG AAAATGAGGA TGAAGAAATG 
GAAGAATTTG AAGAAGGCCC TGTGGATGTC CAGACTTCCC TCCAGGCTAA CACTGAAGCT 
ACTGAAGAAA ATGAACATGA TGAACAGGTC CTACAACGTG ACTTTAAAAA GACAGCAGAA 
AGCAAAAATG TCCCATTGGA ACGAGAAGCC ACTAGTAAAA ATGACCAAAA TAACTGTCCT 
GTGAAACCCT GTTACCTCAA TATCTTGGAA GATGAGCAAC CTTTAAATAG TGCTGCCCAT 
AAGGAGTCAC CTCCTACTGT TGATTCAACT CAACAGCCTA ACCCTTTGCC GTTACGTTTA 
CCTGAAATGG AACCCTTAGT GCCTAGAGTC AAAGAAGTTA AATCTGCTCA GGAAACTCCT 
GAAAGCTCTC TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA 
GCAGAATCTG GTAATATAAG TCAAAAGTCT GATGAAGAAG ATTTTGTAAA AGTTGAAGAT 
TTACCACTGA AACTGACAAT ATATTCAGAG GCAGATCTAA GAAAGAAAAT GGTAGAAGAA 
GAACAGAAAA ACCATTTATC TGGTGAAATA TGTGAAATGC AGACCGAAGA ATTAGCTGGA 
AATTCTGAGA CACTAAAAGA ACCTGAAACG GTGGGAGCCC AGAGTATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001302436.2
CDS	292..6342
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001315507.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001315507.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGAAAC TCCCGTTATT 
GAAAATCGTA GTTCACAACA ACCTGTAAGT GAAGTTTCTA CCATCCCATG TCCTAGAATT 
GATACTCAGC AGCTGGACCG GCAAATTAAA GCAATTATGA AAGAAGTCAT TCCTTTTTTG 
AAGGAGCACA TGGATGAAGT ATGCTCCTCG CAGCTTCTAA CTTCAGTAAG GCGCATGGTT 
TTGACCCTTA CCCAGCAAAA TGATGAGAGC AAAGAGTTTG TAAAGTTCTT TCATAAACAA 
CTTGGAAGTA TATTACAGGA TTCACTGGCA AAATTTGCTG GCAGAAAACT GAAAGACTGT 
GGAGAAGATC TTCTTGTAGA GATATCTGAA GTGTTGTTCA ATGAATTGGC TTTCTTTAAG 
CTTATGCAAG ATTTGGATAA TAATAGTATA ACTGTTAAAC AGAGATGCAA AAGGAAAATA 
GAAGCAACTG GAGTGATACA ATCTTGTGCC AAAGAGGCTA AAAGGATTCT TGAAGATCAT 
GGCTCACCTG CTGGAGAGAT TGATGATGAA GACAAAGACA AGGATGAAAC TGAAACAGTT 
AAGCAGACTC AAACATCTGA GGTGTATGAT GGTCCCAAAA ATGTAAGATC TGATATTTCT 
GATCAAGAGG AAGATGAAGA AAGTGAAGGA TGTCCAGTGT CTATTAATTT GTCTAAAGCT 
GAAACTCAGG CTTTAACTAA TTATGGAAGT GGAGAAGATG AAAATGAGGA TGAAGAAATG 
GAAGAATTTG AAGAAGGCCC TGTGGATGTC CAGACTTCCC TCCAGGCTAA CACTGAAGCT 
ACTGAAGAAA ATGAACATGA TGAACAGGTC CTACAACGTG ACTTTAAAAA GACAGCAGAA 
AGCAAAAATG TCCCATTGGA ACGAGAAGCC ACTAGTAAAA ATGACCAAAA TAACTGTCCT 
GTGAAACCCT GTTACCTCAA TATCTTGGAA GATGAGCAAC CTTTAAATAG TGCTGCCCAT 
AAGGAGTCAC CTCCTACTGT TGATTCAACT CAACAGCCTA ACCCTTTGCC GTTACGTTTA 
CCTGAAATGG AACCCTTAGT GCCTAGAGTC AAAGAAGTTA AATCTGCTCA GGAAACTCCT 
GAAAGCTCTC TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA 
GCAGAATCTG GTAATATAAG TCAAAAGTCT GATGAAGAAG ATTTTGTAAA AGTTGAAGAT 
TTACCACTGA AACTGACAAT ATATTCAGAG GCAGATCTAA GAAAGAAAAT GGTAGAAGAA 
GAACAGAAAA ACCATTTATC TGGTGAAATA TGTGAAATGC AGACCGAAGA ATTAGCTGGA 
AATTCTGAGA CACTAAAAGA ACCTGAAACG GTGGGAGCCC AGAGTATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352632.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339561.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 4, mRNA.

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CloneID	OHu98018
Clone ID Related Accession (Same CDS sequence)	XM_017013474.1 , NM_001352632.2
Accession Version	NM_001352632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352632.1. Transcript Variant: This variant (4) encodes the longest isoform (4). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.246957.1, SRR1803612.19531.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGATTTT TCTCAGGAGC ACATGGATGA AGTATGCTCC 
TCGCAGCTTC TAACTTCAGT AAGGCGCATG GTTTTGACCC TTACCCAGCA AAATGATGAG 
AGCAAAGAGT TTGTAAAGTT CTTTCATAAA CAACTTGGAA GTATATTACA GGATTCACTG 
GCAAAATTTG CTGGCAGAAA ACTGAAAGAC TGTGGAGAAG ATCTTCTTGT AGAGATATCT 
GAAGTGTTGT TCAATGAATT GGCTTTCTTT AAGCTTATGC AAGATTTGGA TAATAATAGT 
ATAACTGTTA AACAGAGATG CAAAAGGAAA ATAGAAGCAA CTGGAGTGAT ACAATCTTGT 
GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT CATGGCTCAC CTGCTGGAGA GATTGATGAT 
GAAGACAAAG ACAAGGATGA AACTGAAACA GTTAAGCAGA CTCAAACATC TGAGGTGTAT 
GATGGTCCCA AAAATGTAAG ATCTGATATT TCTGATCAAG AGGAAGATGA AGAAAGTGAA 
GGATGTCCAG TGTCTATTAA TTTGTCTAAA GCTGAAACTC AGGCTTTAAC TAATTATGGA 
AGTGGAGAAG ATGAAAATGA GGATGAAGAA ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT 
GTCCAGACTT CCCTCCAGGC TAACACTGAA GCTACTGAAG AAAATGAACA TGATGAACAG 
GTCCTACAAC GTGACTTTAA AAAGACAGCA GAAAGCAAAA ATGTCCCATT GGAACGAGAA 
GCCACTAGTA AAAATGACCA AAATAACTGT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAGATGAGC AACCTTTAAA TAGTGCTGCC CATAAGGAGT CACCTCCTAC TGTTGATTCA 
ACTCAACAGC CTAACCCTTT GCCGTTACGT TTACCTGAAA TGGAACCCTT AGTGCCTAGA 
GTCAAAGAAG TTAAATCTGC TCAGGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAATAT AAGTCAAAAG 
TCTGATGAAG AAGATTTTGT AAAAGTTGAA GATTTACCAC TGAAACTGAC AATATATTCA 
GAGGCAGATC TAAGAAAGAA AATGGTAGAA GAAGAACAGA AAAACCATTT ATCTGGTGAA 
ATATGTGAAA TGCAGACCGA AGAATTAGCT GGAAATTCTG AGACACTAAA AGAACCTGAA 
ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339561.2
CDS	292..6495
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352632.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352632.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGATTTT TCTCAGGAGC ACATGGATGA AGTATGCTCC 
TCGCAGCTTC TAACTTCAGT AAGGCGCATG GTTTTGACCC TTACCCAGCA AAATGATGAG 
AGCAAAGAGT TTGTAAAGTT CTTTCATAAA CAACTTGGAA GTATATTACA GGATTCACTG 
GCAAAATTTG CTGGCAGAAA ACTGAAAGAC TGTGGAGAAG ATCTTCTTGT AGAGATATCT 
GAAGTGTTGT TCAATGAATT GGCTTTCTTT AAGCTTATGC AAGATTTGGA TAATAATAGT 
ATAACTGTTA AACAGAGATG CAAAAGGAAA ATAGAAGCAA CTGGAGTGAT ACAATCTTGT 
GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT CATGGCTCAC CTGCTGGAGA GATTGATGAT 
GAAGACAAAG ACAAGGATGA AACTGAAACA GTTAAGCAGA CTCAAACATC TGAGGTGTAT 
GATGGTCCCA AAAATGTAAG ATCTGATATT TCTGATCAAG AGGAAGATGA AGAAAGTGAA 
GGATGTCCAG TGTCTATTAA TTTGTCTAAA GCTGAAACTC AGGCTTTAAC TAATTATGGA 
AGTGGAGAAG ATGAAAATGA GGATGAAGAA ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT 
GTCCAGACTT CCCTCCAGGC TAACACTGAA GCTACTGAAG AAAATGAACA TGATGAACAG 
GTCCTACAAC GTGACTTTAA AAAGACAGCA GAAAGCAAAA ATGTCCCATT GGAACGAGAA 
GCCACTAGTA AAAATGACCA AAATAACTGT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAGATGAGC AACCTTTAAA TAGTGCTGCC CATAAGGAGT CACCTCCTAC TGTTGATTCA 
ACTCAACAGC CTAACCCTTT GCCGTTACGT TTACCTGAAA TGGAACCCTT AGTGCCTAGA 
GTCAAAGAAG TTAAATCTGC TCAGGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAATAT AAGTCAAAAG 
TCTGATGAAG AAGATTTTGT AAAAGTTGAA GATTTACCAC TGAAACTGAC AATATATTCA 
GAGGCAGATC TAAGAAAGAA AATGGTAGAA GAAGAACAGA AAAACCATTT ATCTGGTGAA 
ATATGTGAAA TGCAGACCGA AGAATTAGCT GGAAATTCTG AGACACTAAA AGAACCTGAA 
ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352651.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339580.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 23, mRNA.

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CloneID	OHu98019
Clone ID Related Accession (Same CDS sequence)	XM_017013476.1 , XM_024447168.1 , XM_017013475.1 , NM_001352651.2 , NM_001352650.2
Accession Version	NM_001352651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 19
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 3, 2019 this sequence version replaced NM_001352651.1. Transcript Variant: This variant (23), as well as variant 22, encodes isoform 19. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.211119.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCCTC GCAGCTTCTA 
ACTTCAGTAA GGCGCATGGT TTTGACCCTT ACCCAGCAAA ATGATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTC 
AATGAATTGG CTTTCTTTAA GCTTATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCAACT GGAGTGATAC AATCTTGTGC CAAAGAGGCT 
AAAAGGATTC TTGAAGATCA TGGCTCACCT GCTGGAGAGA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCAGACT CAAACATCTG AGGTGTATGA TGGTCCCAAA 
AATGTAAGAT CTGATATTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGG ATGTCCAGTG 
TCTATTAATT TGTCTAAAGC TGAAACTCAG GCTTTAACTA ATTATGGAAG TGGAGAAGAT 
GAAAATGAGG ATGAAGAAAT GGAAGAATTT GAAGAAGGCC CTGTGGATGT CCAGACTTCC 
CTCCAGGCTA ACACTGAAGC TACTGAAGAA AATGAACATG ATGAACAGGT CCTACAACGT 
GACTTTAAAA AGACAGCAGA AAGCAAAAAT GTCCCATTGG AACGAGAAGC CACTAGTAAA 
AATGACCAAA ATAACTGTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AGATGAGCAA 
CCTTTAAATA GTGCTGCCCA TAAGGAGTCA CCTCCTACTG TTGATTCAAC TCAACAGCCT 
AACCCTTTGC CGTTACGTTT ACCTGAAATG GAACCCTTAG TGCCTAGAGT CAAAGAAGTT 
AAATCTGCTC AGGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAATATAA GTCAAAAGTC TGATGAAGAA 
GATTTTGTAA AAGTTGAAGA TTTACCACTG AAACTGACAA TATATTCAGA GGCAGATCTA 
AGAAAGAAAA TGGTAGAAGA AGAACAGAAA AACCATTTAT CTGGTGAAAT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG AAATTCTGAG ACACTAAAAG AACCTGAAAC GGTGGGAGCC 
CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339580.2
CDS	224..6415
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352651.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352651.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCCTC GCAGCTTCTA 
ACTTCAGTAA GGCGCATGGT TTTGACCCTT ACCCAGCAAA ATGATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTC 
AATGAATTGG CTTTCTTTAA GCTTATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCAACT GGAGTGATAC AATCTTGTGC CAAAGAGGCT 
AAAAGGATTC TTGAAGATCA TGGCTCACCT GCTGGAGAGA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCAGACT CAAACATCTG AGGTGTATGA TGGTCCCAAA 
AATGTAAGAT CTGATATTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGG ATGTCCAGTG 
TCTATTAATT TGTCTAAAGC TGAAACTCAG GCTTTAACTA ATTATGGAAG TGGAGAAGAT 
GAAAATGAGG ATGAAGAAAT GGAAGAATTT GAAGAAGGCC CTGTGGATGT CCAGACTTCC 
CTCCAGGCTA ACACTGAAGC TACTGAAGAA AATGAACATG ATGAACAGGT CCTACAACGT 
GACTTTAAAA AGACAGCAGA AAGCAAAAAT GTCCCATTGG AACGAGAAGC CACTAGTAAA 
AATGACCAAA ATAACTGTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AGATGAGCAA 
CCTTTAAATA GTGCTGCCCA TAAGGAGTCA CCTCCTACTG TTGATTCAAC TCAACAGCCT 
AACCCTTTGC CGTTACGTTT ACCTGAAATG GAACCCTTAG TGCCTAGAGT CAAAGAAGTT 
AAATCTGCTC AGGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAATATAA GTCAAAAGTC TGATGAAGAA 
GATTTTGTAA AAGTTGAAGA TTTACCACTG AAACTGACAA TATATTCAGA GGCAGATCTA 
AGAAAGAAAA TGGTAGAAGA AGAACAGAAA AACCATTTAT CTGGTGAAAT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG AAATTCTGAG ACACTAAAAG AACCTGAAAC GGTGGGAGCC 
CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352650.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339579.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 22, mRNA.

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CloneID	OHu98019
Clone ID Related Accession (Same CDS sequence)	XM_017013476.1 , XM_024447168.1 , XM_017013475.1 , NM_001352651.2 , NM_001352650.2
Accession Version	NM_001352650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 19
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352650.1. Transcript Variant: This variant (22), as well as variant 23, encodes isoform 19. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.26289.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCCTC GCAGCTTCTA 
ACTTCAGTAA GGCGCATGGT TTTGACCCTT ACCCAGCAAA ATGATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTC 
AATGAATTGG CTTTCTTTAA GCTTATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCAACT GGAGTGATAC AATCTTGTGC CAAAGAGGCT 
AAAAGGATTC TTGAAGATCA TGGCTCACCT GCTGGAGAGA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCAGACT CAAACATCTG AGGTGTATGA TGGTCCCAAA 
AATGTAAGAT CTGATATTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGG ATGTCCAGTG 
TCTATTAATT TGTCTAAAGC TGAAACTCAG GCTTTAACTA ATTATGGAAG TGGAGAAGAT 
GAAAATGAGG ATGAAGAAAT GGAAGAATTT GAAGAAGGCC CTGTGGATGT CCAGACTTCC 
CTCCAGGCTA ACACTGAAGC TACTGAAGAA AATGAACATG ATGAACAGGT CCTACAACGT 
GACTTTAAAA AGACAGCAGA AAGCAAAAAT GTCCCATTGG AACGAGAAGC CACTAGTAAA 
AATGACCAAA ATAACTGTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AGATGAGCAA 
CCTTTAAATA GTGCTGCCCA TAAGGAGTCA CCTCCTACTG TTGATTCAAC TCAACAGCCT 
AACCCTTTGC CGTTACGTTT ACCTGAAATG GAACCCTTAG TGCCTAGAGT CAAAGAAGTT 
AAATCTGCTC AGGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAATATAA GTCAAAAGTC TGATGAAGAA 
GATTTTGTAA AAGTTGAAGA TTTACCACTG AAACTGACAA TATATTCAGA GGCAGATCTA 
AGAAAGAAAA TGGTAGAAGA AGAACAGAAA AACCATTTAT CTGGTGAAAT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG AAATTCTGAG ACACTAAAAG AACCTGAAAC GGTGGGAGCC 
CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339579.2
CDS	629..6820
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352650.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352650.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCCTC GCAGCTTCTA 
ACTTCAGTAA GGCGCATGGT TTTGACCCTT ACCCAGCAAA ATGATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTC 
AATGAATTGG CTTTCTTTAA GCTTATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCAACT GGAGTGATAC AATCTTGTGC CAAAGAGGCT 
AAAAGGATTC TTGAAGATCA TGGCTCACCT GCTGGAGAGA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCAGACT CAAACATCTG AGGTGTATGA TGGTCCCAAA 
AATGTAAGAT CTGATATTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGG ATGTCCAGTG 
TCTATTAATT TGTCTAAAGC TGAAACTCAG GCTTTAACTA ATTATGGAAG TGGAGAAGAT 
GAAAATGAGG ATGAAGAAAT GGAAGAATTT GAAGAAGGCC CTGTGGATGT CCAGACTTCC 
CTCCAGGCTA ACACTGAAGC TACTGAAGAA AATGAACATG ATGAACAGGT CCTACAACGT 
GACTTTAAAA AGACAGCAGA AAGCAAAAAT GTCCCATTGG AACGAGAAGC CACTAGTAAA 
AATGACCAAA ATAACTGTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AGATGAGCAA 
CCTTTAAATA GTGCTGCCCA TAAGGAGTCA CCTCCTACTG TTGATTCAAC TCAACAGCCT 
AACCCTTTGC CGTTACGTTT ACCTGAAATG GAACCCTTAG TGCCTAGAGT CAAAGAAGTT 
AAATCTGCTC AGGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAATATAA GTCAAAAGTC TGATGAAGAA 
GATTTTGTAA AAGTTGAAGA TTTACCACTG AAACTGACAA TATATTCAGA GGCAGATCTA 
AGAAAGAAAA TGGTAGAAGA AGAACAGAAA AACCATTTAT CTGGTGAAAT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG AAATTCTGAG ACACTAAAAG AACCTGAAAC GGTGGGAGCC 
CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352633.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339562.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 5, mRNA.

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CloneID	OHu98020
Clone ID Related Accession (Same CDS sequence)	NM_001352633.2 , XM_017013477.1
Accession Version	NM_001352633.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352633.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.36010.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA TTGTCACTTT TTTTACTCAA 
GGGTATGAAA GTGCCAGTAT GTCTAGCACA TGTGAACCTT GCAAAAGTAG GAACAGACAT 
TCAGCCCAGA CTGAAGAGCC TGTTCAAGCA AAAGTATTCA GCAGAAAGAA TCATGAGCAA 
CTGGAAAAAA TAATAAAATG TAATAGGTCT ACAGAAATAT CTTCAGAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339562.2
CDS	629..6817
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIIVTFFTQGYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352633.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352633.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA TTGTCACTTT TTTTACTCAA 
GGGTATGAAA GTGCCAGTAT GTCTAGCACA TGTGAACCTT GCAAAAGTAG GAACAGACAT 
TCAGCCCAGA CTGAAGAGCC TGTTCAAGCA AAAGTATTCA GCAGAAAGAA TCATGAGCAA 
CTGGAAAAAA TAATAAAATG TAATAGGTCT ACAGAAATAT CTTCAGAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352634.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339563.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 6, mRNA.

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CloneID	OHu98023
Clone ID Related Accession (Same CDS sequence)	XM_017013482.1 , NM_001352634.2
Accession Version	NM_001352634.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352634.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.198445.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAT TTTTCTCAGG AGCACATGGA TGAAGTATGC 
TCCTCGCAGC TTCTAACTTC AGTAAGGCGC ATGGTTTTGA CCCTTACCCA GCAAAATGAT 
GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT AAACAACTTG GAAGTATATT ACAGGATTCA 
CTGGCAAAAT TTGCTGGCAG AAAACTGAAA GACTGTGGAG AAGATCTTCT TGTAGAGATA 
TCTGAAGTGT TGTTCAATGA ATTGGCTTTC TTTAAGCTTA TGCAAGATTT GGATAATAAT 
AGTATAACTG TTAAACAGAG ATGCAAAAGG AAAATAGAAG CAACTGGAGT GATACAATCT 
TGTGCCAAAG AGGCTAAAAG GATTCTTGAA GATCATGGCT CACCTGCTGG AGAGATTGAT 
GATGAAGACA AAGACAAGGA TGAAACTGAA ACAGTTAAGC AGACTCAAAC ATCTGAGGTG 
TATGATGGTC CCAAAAATGT AAGATCTGAT ATTTCTGATC AAGAGGAAGA TGAAGAAAGT 
GAAGGATGTC CAGTGTCTAT TAATTTGTCT AAAGCTGAAA CTCAGGCTTT AACTAATTAT 
GGAAGTGGAG AAGATGAAAA TGAGGATGAA GAAATGGAAG AATTTGAAGA AGGCCCTGTG 
GATGTCCAGA CTTCCCTCCA GGCTAACACT GAAGCTACTG AAGAAAATGA ACATGATGAA 
CAGGTCCTAC AACGTGACTT TAAAAAGACA GCAGAAAGCA AAAATGTCCC ATTGGAACGA 
GAAGCCACTA GTAAAAATGA CCAAAATAAC TGTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAGATG AGCAACCTTT AAATAGTGCT GCCCATAAGG AGTCACCTCC TACTGTTGAT 
TCAACTCAAC AGCCTAACCC TTTGCCGTTA CGTTTACCTG AAATGGAACC CTTAGTGCCT 
AGAGTCAAAG AAGTTAAATC TGCTCAGGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA TATAAGTCAA 
AAGTCTGATG AAGAAGATTT TGTAAAAGTT GAAGATTTAC CACTGAAACT GACAATATAT 
TCAGAGGCAG ATCTAAGAAA GAAAATGGTA GAAGAAGAAC AGAAAAACCA TTTATCTGGT 
GAAATATGTG AAATGCAGAC CGAAGAATTA GCTGGAAATT CTGAGACACT AAAAGAACCT 
GAAACGGTGG GAGCCCAGAG TATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339563.2
CDS	292..6378
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352634.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352634.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAT TTTTCTCAGG AGCACATGGA TGAAGTATGC 
TCCTCGCAGC TTCTAACTTC AGTAAGGCGC ATGGTTTTGA CCCTTACCCA GCAAAATGAT 
GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT AAACAACTTG GAAGTATATT ACAGGATTCA 
CTGGCAAAAT TTGCTGGCAG AAAACTGAAA GACTGTGGAG AAGATCTTCT TGTAGAGATA 
TCTGAAGTGT TGTTCAATGA ATTGGCTTTC TTTAAGCTTA TGCAAGATTT GGATAATAAT 
AGTATAACTG TTAAACAGAG ATGCAAAAGG AAAATAGAAG CAACTGGAGT GATACAATCT 
TGTGCCAAAG AGGCTAAAAG GATTCTTGAA GATCATGGCT CACCTGCTGG AGAGATTGAT 
GATGAAGACA AAGACAAGGA TGAAACTGAA ACAGTTAAGC AGACTCAAAC ATCTGAGGTG 
TATGATGGTC CCAAAAATGT AAGATCTGAT ATTTCTGATC AAGAGGAAGA TGAAGAAAGT 
GAAGGATGTC CAGTGTCTAT TAATTTGTCT AAAGCTGAAA CTCAGGCTTT AACTAATTAT 
GGAAGTGGAG AAGATGAAAA TGAGGATGAA GAAATGGAAG AATTTGAAGA AGGCCCTGTG 
GATGTCCAGA CTTCCCTCCA GGCTAACACT GAAGCTACTG AAGAAAATGA ACATGATGAA 
CAGGTCCTAC AACGTGACTT TAAAAAGACA GCAGAAAGCA AAAATGTCCC ATTGGAACGA 
GAAGCCACTA GTAAAAATGA CCAAAATAAC TGTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAGATG AGCAACCTTT AAATAGTGCT GCCCATAAGG AGTCACCTCC TACTGTTGAT 
TCAACTCAAC AGCCTAACCC TTTGCCGTTA CGTTTACCTG AAATGGAACC CTTAGTGCCT 
AGAGTCAAAG AAGTTAAATC TGCTCAGGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA TATAAGTCAA 
AAGTCTGATG AAGAAGATTT TGTAAAAGTT GAAGATTTAC CACTGAAACT GACAATATAT 
TCAGAGGCAG ATCTAAGAAA GAAAATGGTA GAAGAAGAAC AGAAAAACCA TTTATCTGGT 
GAAATATGTG AAATGCAGAC CGAAGAATTA GCTGGAAATT CTGAGACACT AAAAGAACCT 
GAAACGGTGG GAGCCCAGAG TATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352652.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339581.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 24, mRNA.

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CloneID	OHu98024
Clone ID Related Accession (Same CDS sequence)	XM_017013483.1 , NM_001352652.2 , XM_017013484.1 , NM_001352653.2
Accession Version	NM_001352652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 20
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352652.1. Transcript Variant: This variant (24), as well as variant 25, encodes isoform 20. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.129267.1, SRR1803616.20706.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC CTCGCAGCTT 
CTAACTTCAG TAAGGCGCAT GGTTTTGACC CTTACCCAGC AAAATGATGA GAGCAAAGAG 
TTTGTAAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTCAATGAAT TGGCTTTCTT TAAGCTTATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAGAT GCAAAAGGAA AATAGAAGCA ACTGGAGTGA TACAATCTTG TGCCAAAGAG 
GCTAAAAGGA TTCTTGAAGA TCATGGCTCA CCTGCTGGAG AGATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCAG ACTCAAACAT CTGAGGTGTA TGATGGTCCC 
AAAAATGTAA GATCTGATAT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGGATGTCCA 
GTGTCTATTA ATTTGTCTAA AGCTGAAACT CAGGCTTTAA CTAATTATGG AAGTGGAGAA 
GATGAAAATG AGGATGAAGA AATGGAAGAA TTTGAAGAAG GCCCTGTGGA TGTCCAGACT 
TCCCTCCAGG CTAACACTGA AGCTACTGAA GAAAATGAAC ATGATGAACA GGTCCTACAA 
CGTGACTTTA AAAAGACAGC AGAAAGCAAA AATGTCCCAT TGGAACGAGA AGCCACTAGT 
AAAAATGACC AAAATAACTG TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAGATGAG 
CAACCTTTAA ATAGTGCTGC CCATAAGGAG TCACCTCCTA CTGTTGATTC AACTCAACAG 
CCTAACCCTT TGCCGTTACG TTTACCTGAA ATGGAACCCT TAGTGCCTAG AGTCAAAGAA 
GTTAAATCTG CTCAGGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAATA TAAGTCAAAA GTCTGATGAA 
GAAGATTTTG TAAAAGTTGA AGATTTACCA CTGAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGAAAGA AAATGGTAGA AGAAGAACAG AAAAACCATT TATCTGGTGA AATATGTGAA 
ATGCAGACCG AAGAATTAGC TGGAAATTCT GAGACACTAA AAGAACCTGA AACGGTGGGA 
GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339581.2
CDS	224..6298
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352652.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352652.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC CTCGCAGCTT 
CTAACTTCAG TAAGGCGCAT GGTTTTGACC CTTACCCAGC AAAATGATGA GAGCAAAGAG 
TTTGTAAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTCAATGAAT TGGCTTTCTT TAAGCTTATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAGAT GCAAAAGGAA AATAGAAGCA ACTGGAGTGA TACAATCTTG TGCCAAAGAG 
GCTAAAAGGA TTCTTGAAGA TCATGGCTCA CCTGCTGGAG AGATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCAG ACTCAAACAT CTGAGGTGTA TGATGGTCCC 
AAAAATGTAA GATCTGATAT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGGATGTCCA 
GTGTCTATTA ATTTGTCTAA AGCTGAAACT CAGGCTTTAA CTAATTATGG AAGTGGAGAA 
GATGAAAATG AGGATGAAGA AATGGAAGAA TTTGAAGAAG GCCCTGTGGA TGTCCAGACT 
TCCCTCCAGG CTAACACTGA AGCTACTGAA GAAAATGAAC ATGATGAACA GGTCCTACAA 
CGTGACTTTA AAAAGACAGC AGAAAGCAAA AATGTCCCAT TGGAACGAGA AGCCACTAGT 
AAAAATGACC AAAATAACTG TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAGATGAG 
CAACCTTTAA ATAGTGCTGC CCATAAGGAG TCACCTCCTA CTGTTGATTC AACTCAACAG 
CCTAACCCTT TGCCGTTACG TTTACCTGAA ATGGAACCCT TAGTGCCTAG AGTCAAAGAA 
GTTAAATCTG CTCAGGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAATA TAAGTCAAAA GTCTGATGAA 
GAAGATTTTG TAAAAGTTGA AGATTTACCA CTGAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGAAAGA AAATGGTAGA AGAAGAACAG AAAAACCATT TATCTGGTGA AATATGTGAA 
ATGCAGACCG AAGAATTAGC TGGAAATTCT GAGACACTAA AAGAACCTGA AACGGTGGGA 
GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352653.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339582.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 25, mRNA.

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CloneID	OHu98024
Clone ID Related Accession (Same CDS sequence)	XM_017013483.1 , NM_001352652.2 , XM_017013484.1 , NM_001352653.2
Accession Version	NM_001352653.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 20
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352653.1. Transcript Variant: This variant (25), as well as variant 24, encodes isoform 20. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.218103.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC CTCGCAGCTT 
CTAACTTCAG TAAGGCGCAT GGTTTTGACC CTTACCCAGC AAAATGATGA GAGCAAAGAG 
TTTGTAAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTCAATGAAT TGGCTTTCTT TAAGCTTATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAGAT GCAAAAGGAA AATAGAAGCA ACTGGAGTGA TACAATCTTG TGCCAAAGAG 
GCTAAAAGGA TTCTTGAAGA TCATGGCTCA CCTGCTGGAG AGATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCAG ACTCAAACAT CTGAGGTGTA TGATGGTCCC 
AAAAATGTAA GATCTGATAT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGGATGTCCA 
GTGTCTATTA ATTTGTCTAA AGCTGAAACT CAGGCTTTAA CTAATTATGG AAGTGGAGAA 
GATGAAAATG AGGATGAAGA AATGGAAGAA TTTGAAGAAG GCCCTGTGGA TGTCCAGACT 
TCCCTCCAGG CTAACACTGA AGCTACTGAA GAAAATGAAC ATGATGAACA GGTCCTACAA 
CGTGACTTTA AAAAGACAGC AGAAAGCAAA AATGTCCCAT TGGAACGAGA AGCCACTAGT 
AAAAATGACC AAAATAACTG TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAGATGAG 
CAACCTTTAA ATAGTGCTGC CCATAAGGAG TCACCTCCTA CTGTTGATTC AACTCAACAG 
CCTAACCCTT TGCCGTTACG TTTACCTGAA ATGGAACCCT TAGTGCCTAG AGTCAAAGAA 
GTTAAATCTG CTCAGGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAATA TAAGTCAAAA GTCTGATGAA 
GAAGATTTTG TAAAAGTTGA AGATTTACCA CTGAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGAAAGA AAATGGTAGA AGAAGAACAG AAAAACCATT TATCTGGTGA AATATGTGAA 
ATGCAGACCG AAGAATTAGC TGGAAATTCT GAGACACTAA AAGAACCTGA AACGGTGGGA 
GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339582.2
CDS	629..6703
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352653.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352653.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC CTCGCAGCTT 
CTAACTTCAG TAAGGCGCAT GGTTTTGACC CTTACCCAGC AAAATGATGA GAGCAAAGAG 
TTTGTAAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTCAATGAAT TGGCTTTCTT TAAGCTTATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAGAT GCAAAAGGAA AATAGAAGCA ACTGGAGTGA TACAATCTTG TGCCAAAGAG 
GCTAAAAGGA TTCTTGAAGA TCATGGCTCA CCTGCTGGAG AGATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCAG ACTCAAACAT CTGAGGTGTA TGATGGTCCC 
AAAAATGTAA GATCTGATAT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGGATGTCCA 
GTGTCTATTA ATTTGTCTAA AGCTGAAACT CAGGCTTTAA CTAATTATGG AAGTGGAGAA 
GATGAAAATG AGGATGAAGA AATGGAAGAA TTTGAAGAAG GCCCTGTGGA TGTCCAGACT 
TCCCTCCAGG CTAACACTGA AGCTACTGAA GAAAATGAAC ATGATGAACA GGTCCTACAA 
CGTGACTTTA AAAAGACAGC AGAAAGCAAA AATGTCCCAT TGGAACGAGA AGCCACTAGT 
AAAAATGACC AAAATAACTG TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAGATGAG 
CAACCTTTAA ATAGTGCTGC CCATAAGGAG TCACCTCCTA CTGTTGATTC AACTCAACAG 
CCTAACCCTT TGCCGTTACG TTTACCTGAA ATGGAACCCT TAGTGCCTAG AGTCAAAGAA 
GTTAAATCTG CTCAGGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAATA TAAGTCAAAA GTCTGATGAA 
GAAGATTTTG TAAAAGTTGA AGATTTACCA CTGAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGAAAGA AAATGGTAGA AGAAGAACAG AAAAACCATT TATCTGGTGA AATATGTGAA 
ATGCAGACCG AAGAATTAGC TGGAAATTCT GAGACACTAA AAGAACCTGA AACGGTGGGA 
GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352636.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339565.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 8, mRNA.

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CloneID	OHu98027
Clone ID Related Accession (Same CDS sequence)	NM_001352636.2
Accession Version	NM_001352636.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352636.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.5959.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GCAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339565.2
CDS	292..6321
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352636.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352636.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GCAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352637.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339566.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 9, mRNA.

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CloneID	OHu98028
Clone ID Related Accession (Same CDS sequence)	XM_017013488.1 , NM_001352637.2 , XM_017013489.1
Accession Version	NM_001352637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352637.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.89475.1, SRR1803613.51435.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGGT 
ACTACAGTTA ATAATTTAGA AGAAACTCCC GTTATTGAAA ATCGTAGTTC ACAACAACCT 
GTAAGTGAAG TTTCTACCAT CCCATGTCCT AGAATTGATA CTCAGCAGCT GGACCGGCAA 
ATTAAAGCAA TTATGAAAGA AGTCATTCCT TTTTTGAAGG AGCACATGGA TGAAGTATGC 
TCCTCGCAGC TTCTAACTTC AGTAAGGCGC ATGGTTTTGA CCCTTACCCA GCAAAATGAT 
GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT AAACAACTTG GAAGTATATT ACAGGATTCA 
CTGGCAAAAT TTGCTGGCAG AAAACTGAAA GACTGTGGAG AAGATCTTCT TGTAGAGATA 
TCTGAAGTGT TGTTCAATGA ATTGGCTTTC TTTAAGCTTA TGCAAGATTT GGATAATAAT 
AGTATAACTG TTAAACAGAG ATGCAAAAGG AAAATAGAAG CAACTGGAGT GATACAATCT 
TGTGCCAAAG AGGCTAAAAG GATTCTTGAA GATCATGGCT CACCTGCTGG AGAGATTGAT 
GATGAAGACA AAGACAAGGA TGAAACTGAA ACAGTTAAGC AGACTCAAAC ATCTGAGGTG 
TATGATGGTC CCAAAAATGT AAGATCTGAT ATTTCTGATC AAGAGGAAGA TGAAGAAAGT 
GAAGGATGTC CAGTGTCTAT TAATTTGTCT AAAGCTGAAA CTCAGGCTTT AACTAATTAT 
GGAAGTGGAG AAGATGAAAA TGAGGATGAA GAAATGGAAG AATTTGAAGA AGGCCCTGTG 
GATGTCCAGA CTTCCCTCCA GGCTAACACT GAAGCTACTG AAGAAAATGA ACATGATGAA 
CAGGTCCTAC AACGTGACTT TAAAAAGACA GCAGAAAGCA AAAATGTCCC ATTGGAACGA 
GAAGCCACTA GTAAAAATGA CCAAAATAAC TGTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAGATG AGCAACCTTT AAATAGTGCT GCCCATAAGG AGTCACCTCC TACTGTTGAT 
TCAACTCAAC AGCCTAACCC TTTGCCGTTA CGTTTACCTG AAATGGAACC CTTAGTGCCT 
AGAGTCAAAG AAGTTAAATC TGCTCAGGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA TATAAGTCAA 
AAGTCTGATG AAGAAGATTT TGTAAAAGTT GAAGATTTAC CACTGAAACT GACAATATAT 
TCAGAGGCAG ATCTAAGAAA GAAAATGGTA GAAGAAGAAC AGAAAAACCA TTTATCTGGT 
GAAATATGTG AAATGCAGAC CGAAGAATTA GCTGGAAATT CTGAGACACT AAAAGAACCT 
GAAACGGTGG GAGCCCAGAG TATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339566.2
CDS	292..6318
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352637.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352637.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGGT 
ACTACAGTTA ATAATTTAGA AGAAACTCCC GTTATTGAAA ATCGTAGTTC ACAACAACCT 
GTAAGTGAAG TTTCTACCAT CCCATGTCCT AGAATTGATA CTCAGCAGCT GGACCGGCAA 
ATTAAAGCAA TTATGAAAGA AGTCATTCCT TTTTTGAAGG AGCACATGGA TGAAGTATGC 
TCCTCGCAGC TTCTAACTTC AGTAAGGCGC ATGGTTTTGA CCCTTACCCA GCAAAATGAT 
GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT AAACAACTTG GAAGTATATT ACAGGATTCA 
CTGGCAAAAT TTGCTGGCAG AAAACTGAAA GACTGTGGAG AAGATCTTCT TGTAGAGATA 
TCTGAAGTGT TGTTCAATGA ATTGGCTTTC TTTAAGCTTA TGCAAGATTT GGATAATAAT 
AGTATAACTG TTAAACAGAG ATGCAAAAGG AAAATAGAAG CAACTGGAGT GATACAATCT 
TGTGCCAAAG AGGCTAAAAG GATTCTTGAA GATCATGGCT CACCTGCTGG AGAGATTGAT 
GATGAAGACA AAGACAAGGA TGAAACTGAA ACAGTTAAGC AGACTCAAAC ATCTGAGGTG 
TATGATGGTC CCAAAAATGT AAGATCTGAT ATTTCTGATC AAGAGGAAGA TGAAGAAAGT 
GAAGGATGTC CAGTGTCTAT TAATTTGTCT AAAGCTGAAA CTCAGGCTTT AACTAATTAT 
GGAAGTGGAG AAGATGAAAA TGAGGATGAA GAAATGGAAG AATTTGAAGA AGGCCCTGTG 
GATGTCCAGA CTTCCCTCCA GGCTAACACT GAAGCTACTG AAGAAAATGA ACATGATGAA 
CAGGTCCTAC AACGTGACTT TAAAAAGACA GCAGAAAGCA AAAATGTCCC ATTGGAACGA 
GAAGCCACTA GTAAAAATGA CCAAAATAAC TGTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAGATG AGCAACCTTT AAATAGTGCT GCCCATAAGG AGTCACCTCC TACTGTTGAT 
TCAACTCAAC AGCCTAACCC TTTGCCGTTA CGTTTACCTG AAATGGAACC CTTAGTGCCT 
AGAGTCAAAG AAGTTAAATC TGCTCAGGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA TATAAGTCAA 
AAGTCTGATG AAGAAGATTT TGTAAAAGTT GAAGATTTAC CACTGAAACT GACAATATAT 
TCAGAGGCAG ATCTAAGAAA GAAAATGGTA GAAGAAGAAC AGAAAAACCA TTTATCTGGT 
GAAATATGTG AAATGCAGAC CGAAGAATTA GCTGGAAATT CTGAGACACT AAAAGAACCT 
GAAACGGTGG GAGCCCAGAG TATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352638.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339567.2 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 10, mRNA.

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CloneID	OHu98029
Clone ID Related Accession (Same CDS sequence)	NM_001352638.2 , XM_017013490.1 , XM_017013490.2
Accession Version	NM_001352638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5 and AC087625.12. On Jun 2, 2019 this sequence version replaced NM_001352638.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.55097.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149178, SAMN02400288 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGAA 
ACTCCCGTTA TTGAAAATCG TAGTTCACAA CAACCTGTAA GTGAAGTTTC TACCATCCCA 
TGTCCTAGAA TTGATACTCA GCAGCTGGAC CGGCAAATTA AAGCAATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGCTCCT CGCAGCTTCT AACTTCAGTA 
AGGCGCATGG TTTTGACCCT TACCCAGCAA AATGATGAGA GCAAAGAGTT TGTAAAGTTC 
TTTCATAAAC AACTTGGAAG TATATTACAG GATTCACTGG CAAAATTTGC TGGCAGAAAA 
CTGAAAGACT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTGTT CAATGAATTG 
GCTTTCTTTA AGCTTATGCA AGATTTGGAT AATAATAGTA TAACTGTTAA ACAGAGATGC 
AAAAGGAAAA TAGAAGCAAC TGGAGTGATA CAATCTTGTG CCAAAGAGGC TAAAAGGATT 
CTTGAAGATC ATGGCTCACC TGCTGGAGAG ATTGATGATG AAGACAAAGA CAAGGATGAA 
ACTGAAACAG TTAAGCAGAC TCAAACATCT GAGGTGTATG ATGGTCCCAA AAATGTAAGA 
TCTGATATTT CTGATCAAGA GGAAGATGAA GAAAGTGAAG GATGTCCAGT GTCTATTAAT 
TTGTCTAAAG CTGAAACTCA GGCTTTAACT AATTATGGAA GTGGAGAAGA TGAAAATGAG 
GATGAAGAAA TGGAAGAATT TGAAGAAGGC CCTGTGGATG TCCAGACTTC CCTCCAGGCT 
AACACTGAAG CTACTGAAGA AAATGAACAT GATGAACAGG TCCTACAACG TGACTTTAAA 
AAGACAGCAG AAAGCAAAAA TGTCCCATTG GAACGAGAAG CCACTAGTAA AAATGACCAA 
AATAACTGTC CTGTGAAACC CTGTTACCTC AATATCTTGG AAGATGAGCA ACCTTTAAAT 
AGTGCTGCCC ATAAGGAGTC ACCTCCTACT GTTGATTCAA CTCAACAGCC TAACCCTTTG 
CCGTTACGTT TACCTGAAAT GGAACCCTTA GTGCCTAGAG TCAAAGAAGT TAAATCTGCT 
CAGGAAACTC CTGAAAGCTC TCTGGCTGGA AGTCCTGATA CTGAATCTCC AGTGTTAGTG 
AATGACTATG AAGCAGAATC TGGTAATATA AGTCAAAAGT CTGATGAAGA AGATTTTGTA 
AAAGTTGAAG ATTTACCACT GAAACTGACA ATATATTCAG AGGCAGATCT AAGAAAGAAA 
ATGGTAGAAG AAGAACAGAA AAACCATTTA TCTGGTGAAA TATGTGAAAT GCAGACCGAA 
GAATTAGCTG GAAATTCTGA GACACTAAAA GAACCTGAAA CGGTGGGAGC CCAGAGTATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339567.2
CDS	561..6563
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352638.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352638.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGAA 
ACTCCCGTTA TTGAAAATCG TAGTTCACAA CAACCTGTAA GTGAAGTTTC TACCATCCCA 
TGTCCTAGAA TTGATACTCA GCAGCTGGAC CGGCAAATTA AAGCAATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGCTCCT CGCAGCTTCT AACTTCAGTA 
AGGCGCATGG TTTTGACCCT TACCCAGCAA AATGATGAGA GCAAAGAGTT TGTAAAGTTC 
TTTCATAAAC AACTTGGAAG TATATTACAG GATTCACTGG CAAAATTTGC TGGCAGAAAA 
CTGAAAGACT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTGTT CAATGAATTG 
GCTTTCTTTA AGCTTATGCA AGATTTGGAT AATAATAGTA TAACTGTTAA ACAGAGATGC 
AAAAGGAAAA TAGAAGCAAC TGGAGTGATA CAATCTTGTG CCAAAGAGGC TAAAAGGATT 
CTTGAAGATC ATGGCTCACC TGCTGGAGAG ATTGATGATG AAGACAAAGA CAAGGATGAA 
ACTGAAACAG TTAAGCAGAC TCAAACATCT GAGGTGTATG ATGGTCCCAA AAATGTAAGA 
TCTGATATTT CTGATCAAGA GGAAGATGAA GAAAGTGAAG GATGTCCAGT GTCTATTAAT 
TTGTCTAAAG CTGAAACTCA GGCTTTAACT AATTATGGAA GTGGAGAAGA TGAAAATGAG 
GATGAAGAAA TGGAAGAATT TGAAGAAGGC CCTGTGGATG TCCAGACTTC CCTCCAGGCT 
AACACTGAAG CTACTGAAGA AAATGAACAT GATGAACAGG TCCTACAACG TGACTTTAAA 
AAGACAGCAG AAAGCAAAAA TGTCCCATTG GAACGAGAAG CCACTAGTAA AAATGACCAA 
AATAACTGTC CTGTGAAACC CTGTTACCTC AATATCTTGG AAGATGAGCA ACCTTTAAAT 
AGTGCTGCCC ATAAGGAGTC ACCTCCTACT GTTGATTCAA CTCAACAGCC TAACCCTTTG 
CCGTTACGTT TACCTGAAAT GGAACCCTTA GTGCCTAGAG TCAAAGAAGT TAAATCTGCT 
CAGGAAACTC CTGAAAGCTC TCTGGCTGGA AGTCCTGATA CTGAATCTCC AGTGTTAGTG 
AATGACTATG AAGCAGAATC TGGTAATATA AGTCAAAAGT CTGATGAAGA AGATTTTGTA 
AAAGTTGAAG ATTTACCACT GAAACTGACA ATATATTCAG AGGCAGATCT AAGAAAGAAA 
ATGGTAGAAG AAGAACAGAA AAACCATTTA TCTGGTGAAA TATGTGAAAT GCAGACCGAA 
GAATTAGCTG GAAATTCTGA GACACTAAAA GAACCTGAAA CGGTGGGAGC CCAGAGTATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013474.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868963.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X13, mRNA.

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CloneID	OHu98018
Clone ID Related Accession (Same CDS sequence)	XM_017013474.1 , NM_001352632.2
Accession Version	XM_017013474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGATTTT TCTCAGGAGC ACATGGATGA AGTATGCTCC 
TCGCAGCTTC TAACTTCAGT AAGGCGCATG GTTTTGACCC TTACCCAGCA AAATGATGAG 
AGCAAAGAGT TTGTAAAGTT CTTTCATAAA CAACTTGGAA GTATATTACA GGATTCACTG 
GCAAAATTTG CTGGCAGAAA ACTGAAAGAC TGTGGAGAAG ATCTTCTTGT AGAGATATCT 
GAAGTGTTGT TCAATGAATT GGCTTTCTTT AAGCTTATGC AAGATTTGGA TAATAATAGT 
ATAACTGTTA AACAGAGATG CAAAAGGAAA ATAGAAGCAA CTGGAGTGAT ACAATCTTGT 
GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT CATGGCTCAC CTGCTGGAGA GATTGATGAT 
GAAGACAAAG ACAAGGATGA AACTGAAACA GTTAAGCAGA CTCAAACATC TGAGGTGTAT 
GATGGTCCCA AAAATGTAAG ATCTGATATT TCTGATCAAG AGGAAGATGA AGAAAGTGAA 
GGATGTCCAG TGTCTATTAA TTTGTCTAAA GCTGAAACTC AGGCTTTAAC TAATTATGGA 
AGTGGAGAAG ATGAAAATGA GGATGAAGAA ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT 
GTCCAGACTT CCCTCCAGGC TAACACTGAA GCTACTGAAG AAAATGAACA TGATGAACAG 
GTCCTACAAC GTGACTTTAA AAAGACAGCA GAAAGCAAAA ATGTCCCATT GGAACGAGAA 
GCCACTAGTA AAAATGACCA AAATAACTGT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAGATGAGC AACCTTTAAA TAGTGCTGCC CATAAGGAGT CACCTCCTAC TGTTGATTCA 
ACTCAACAGC CTAACCCTTT GCCGTTACGT TTACCTGAAA TGGAACCCTT AGTGCCTAGA 
GTCAAAGAAG TTAAATCTGC TCAGGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAATAT AAGTCAAAAG 
TCTGATGAAG AAGATTTTGT AAAAGTTGAA GATTTACCAC TGAAACTGAC AATATATTCA 
GAGGCAGATC TAAGAAAGAA AATGGTAGAA GAAGAACAGA AAAACCATTT ATCTGGTGAA 
ATATGTGAAA TGCAGACCGA AGAATTAGCT GGAAATTCTG AGACACTAAA AGAACCTGAA 
ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868963.1
CDS	496..6699
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013474.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013474.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGATTTT TCTCAGGAGC ACATGGATGA AGTATGCTCC 
TCGCAGCTTC TAACTTCAGT AAGGCGCATG GTTTTGACCC TTACCCAGCA AAATGATGAG 
AGCAAAGAGT TTGTAAAGTT CTTTCATAAA CAACTTGGAA GTATATTACA GGATTCACTG 
GCAAAATTTG CTGGCAGAAA ACTGAAAGAC TGTGGAGAAG ATCTTCTTGT AGAGATATCT 
GAAGTGTTGT TCAATGAATT GGCTTTCTTT AAGCTTATGC AAGATTTGGA TAATAATAGT 
ATAACTGTTA AACAGAGATG CAAAAGGAAA ATAGAAGCAA CTGGAGTGAT ACAATCTTGT 
GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT CATGGCTCAC CTGCTGGAGA GATTGATGAT 
GAAGACAAAG ACAAGGATGA AACTGAAACA GTTAAGCAGA CTCAAACATC TGAGGTGTAT 
GATGGTCCCA AAAATGTAAG ATCTGATATT TCTGATCAAG AGGAAGATGA AGAAAGTGAA 
GGATGTCCAG TGTCTATTAA TTTGTCTAAA GCTGAAACTC AGGCTTTAAC TAATTATGGA 
AGTGGAGAAG ATGAAAATGA GGATGAAGAA ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT 
GTCCAGACTT CCCTCCAGGC TAACACTGAA GCTACTGAAG AAAATGAACA TGATGAACAG 
GTCCTACAAC GTGACTTTAA AAAGACAGCA GAAAGCAAAA ATGTCCCATT GGAACGAGAA 
GCCACTAGTA AAAATGACCA AAATAACTGT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAGATGAGC AACCTTTAAA TAGTGCTGCC CATAAGGAGT CACCTCCTAC TGTTGATTCA 
ACTCAACAGC CTAACCCTTT GCCGTTACGT TTACCTGAAA TGGAACCCTT AGTGCCTAGA 
GTCAAAGAAG TTAAATCTGC TCAGGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAATAT AAGTCAAAAG 
TCTGATGAAG AAGATTTTGT AAAAGTTGAA GATTTACCAC TGAAACTGAC AATATATTCA 
GAGGCAGATC TAAGAAAGAA AATGGTAGAA GAAGAACAGA AAAACCATTT ATCTGGTGAA 
ATATGTGAAA TGCAGACCGA AGAATTAGCT GGAAATTCTG AGACACTAAA AGAACCTGAA 
ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013476.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868965.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X15, mRNA.

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CloneID	OHu98019
Clone ID Related Accession (Same CDS sequence)	XM_017013476.1 , XM_024447168.1 , XM_017013475.1 , NM_001352651.2 , NM_001352650.2
Accession Version	XM_017013476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCCTC GCAGCTTCTA 
ACTTCAGTAA GGCGCATGGT TTTGACCCTT ACCCAGCAAA ATGATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTC 
AATGAATTGG CTTTCTTTAA GCTTATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCAACT GGAGTGATAC AATCTTGTGC CAAAGAGGCT 
AAAAGGATTC TTGAAGATCA TGGCTCACCT GCTGGAGAGA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCAGACT CAAACATCTG AGGTGTATGA TGGTCCCAAA 
AATGTAAGAT CTGATATTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGG ATGTCCAGTG 
TCTATTAATT TGTCTAAAGC TGAAACTCAG GCTTTAACTA ATTATGGAAG TGGAGAAGAT 
GAAAATGAGG ATGAAGAAAT GGAAGAATTT GAAGAAGGCC CTGTGGATGT CCAGACTTCC 
CTCCAGGCTA ACACTGAAGC TACTGAAGAA AATGAACATG ATGAACAGGT CCTACAACGT 
GACTTTAAAA AGACAGCAGA AAGCAAAAAT GTCCCATTGG AACGAGAAGC CACTAGTAAA 
AATGACCAAA ATAACTGTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AGATGAGCAA 
CCTTTAAATA GTGCTGCCCA TAAGGAGTCA CCTCCTACTG TTGATTCAAC TCAACAGCCT 
AACCCTTTGC CGTTACGTTT ACCTGAAATG GAACCCTTAG TGCCTAGAGT CAAAGAAGTT 
AAATCTGCTC AGGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAATATAA GTCAAAAGTC TGATGAAGAA 
GATTTTGTAA AAGTTGAAGA TTTACCACTG AAACTGACAA TATATTCAGA GGCAGATCTA 
AGAAAGAAAA TGGTAGAAGA AGAACAGAAA AACCATTTAT CTGGTGAAAT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG AAATTCTGAG ACACTAAAAG AACCTGAAAC GGTGGGAGCC 
CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868965.1
CDS	216..6407
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013476.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013476.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCCTC GCAGCTTCTA 
ACTTCAGTAA GGCGCATGGT TTTGACCCTT ACCCAGCAAA ATGATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTC 
AATGAATTGG CTTTCTTTAA GCTTATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCAACT GGAGTGATAC AATCTTGTGC CAAAGAGGCT 
AAAAGGATTC TTGAAGATCA TGGCTCACCT GCTGGAGAGA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCAGACT CAAACATCTG AGGTGTATGA TGGTCCCAAA 
AATGTAAGAT CTGATATTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGG ATGTCCAGTG 
TCTATTAATT TGTCTAAAGC TGAAACTCAG GCTTTAACTA ATTATGGAAG TGGAGAAGAT 
GAAAATGAGG ATGAAGAAAT GGAAGAATTT GAAGAAGGCC CTGTGGATGT CCAGACTTCC 
CTCCAGGCTA ACACTGAAGC TACTGAAGAA AATGAACATG ATGAACAGGT CCTACAACGT 
GACTTTAAAA AGACAGCAGA AAGCAAAAAT GTCCCATTGG AACGAGAAGC CACTAGTAAA 
AATGACCAAA ATAACTGTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AGATGAGCAA 
CCTTTAAATA GTGCTGCCCA TAAGGAGTCA CCTCCTACTG TTGATTCAAC TCAACAGCCT 
AACCCTTTGC CGTTACGTTT ACCTGAAATG GAACCCTTAG TGCCTAGAGT CAAAGAAGTT 
AAATCTGCTC AGGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAATATAA GTCAAAAGTC TGATGAAGAA 
GATTTTGTAA AAGTTGAAGA TTTACCACTG AAACTGACAA TATATTCAGA GGCAGATCTA 
AGAAAGAAAA TGGTAGAAGA AGAACAGAAA AACCATTTAT CTGGTGAAAT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG AAATTCTGAG ACACTAAAAG AACCTGAAAC GGTGGGAGCC 
CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_024447168.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302936.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X11, mRNA.

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CloneID	OHu98019
Clone ID Related Accession (Same CDS sequence)	XM_017013476.1 , XM_024447168.1 , XM_017013475.1 , NM_001352651.2 , NM_001352650.2
Accession Version	XM_024447168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCCTC GCAGCTTCTA 
ACTTCAGTAA GGCGCATGGT TTTGACCCTT ACCCAGCAAA ATGATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTC 
AATGAATTGG CTTTCTTTAA GCTTATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCAACT GGAGTGATAC AATCTTGTGC CAAAGAGGCT 
AAAAGGATTC TTGAAGATCA TGGCTCACCT GCTGGAGAGA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCAGACT CAAACATCTG AGGTGTATGA TGGTCCCAAA 
AATGTAAGAT CTGATATTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGG ATGTCCAGTG 
TCTATTAATT TGTCTAAAGC TGAAACTCAG GCTTTAACTA ATTATGGAAG TGGAGAAGAT 
GAAAATGAGG ATGAAGAAAT GGAAGAATTT GAAGAAGGCC CTGTGGATGT CCAGACTTCC 
CTCCAGGCTA ACACTGAAGC TACTGAAGAA AATGAACATG ATGAACAGGT CCTACAACGT 
GACTTTAAAA AGACAGCAGA AAGCAAAAAT GTCCCATTGG AACGAGAAGC CACTAGTAAA 
AATGACCAAA ATAACTGTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AGATGAGCAA 
CCTTTAAATA GTGCTGCCCA TAAGGAGTCA CCTCCTACTG TTGATTCAAC TCAACAGCCT 
AACCCTTTGC CGTTACGTTT ACCTGAAATG GAACCCTTAG TGCCTAGAGT CAAAGAAGTT 
AAATCTGCTC AGGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAATATAA GTCAAAAGTC TGATGAAGAA 
GATTTTGTAA AAGTTGAAGA TTTACCACTG AAACTGACAA TATATTCAGA GGCAGATCTA 
AGAAAGAAAA TGGTAGAAGA AGAACAGAAA AACCATTTAT CTGGTGAAAT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG AAATTCTGAG ACACTAAAAG AACCTGAAAC GGTGGGAGCC 
CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302936.1
CDS	496..6687
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_024447168.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447168.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCCTC GCAGCTTCTA 
ACTTCAGTAA GGCGCATGGT TTTGACCCTT ACCCAGCAAA ATGATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTC 
AATGAATTGG CTTTCTTTAA GCTTATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCAACT GGAGTGATAC AATCTTGTGC CAAAGAGGCT 
AAAAGGATTC TTGAAGATCA TGGCTCACCT GCTGGAGAGA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCAGACT CAAACATCTG AGGTGTATGA TGGTCCCAAA 
AATGTAAGAT CTGATATTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGG ATGTCCAGTG 
TCTATTAATT TGTCTAAAGC TGAAACTCAG GCTTTAACTA ATTATGGAAG TGGAGAAGAT 
GAAAATGAGG ATGAAGAAAT GGAAGAATTT GAAGAAGGCC CTGTGGATGT CCAGACTTCC 
CTCCAGGCTA ACACTGAAGC TACTGAAGAA AATGAACATG ATGAACAGGT CCTACAACGT 
GACTTTAAAA AGACAGCAGA AAGCAAAAAT GTCCCATTGG AACGAGAAGC CACTAGTAAA 
AATGACCAAA ATAACTGTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AGATGAGCAA 
CCTTTAAATA GTGCTGCCCA TAAGGAGTCA CCTCCTACTG TTGATTCAAC TCAACAGCCT 
AACCCTTTGC CGTTACGTTT ACCTGAAATG GAACCCTTAG TGCCTAGAGT CAAAGAAGTT 
AAATCTGCTC AGGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAATATAA GTCAAAAGTC TGATGAAGAA 
GATTTTGTAA AAGTTGAAGA TTTACCACTG AAACTGACAA TATATTCAGA GGCAGATCTA 
AGAAAGAAAA TGGTAGAAGA AGAACAGAAA AACCATTTAT CTGGTGAAAT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG AAATTCTGAG ACACTAAAAG AACCTGAAAC GGTGGGAGCC 
CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013475.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868964.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X14, mRNA.

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CloneID	OHu98019
Clone ID Related Accession (Same CDS sequence)	XM_017013476.1 , XM_024447168.1 , XM_017013475.1 , NM_001352651.2 , NM_001352650.2
Accession Version	XM_017013475.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCCTC GCAGCTTCTA 
ACTTCAGTAA GGCGCATGGT TTTGACCCTT ACCCAGCAAA ATGATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTC 
AATGAATTGG CTTTCTTTAA GCTTATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCAACT GGAGTGATAC AATCTTGTGC CAAAGAGGCT 
AAAAGGATTC TTGAAGATCA TGGCTCACCT GCTGGAGAGA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCAGACT CAAACATCTG AGGTGTATGA TGGTCCCAAA 
AATGTAAGAT CTGATATTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGG ATGTCCAGTG 
TCTATTAATT TGTCTAAAGC TGAAACTCAG GCTTTAACTA ATTATGGAAG TGGAGAAGAT 
GAAAATGAGG ATGAAGAAAT GGAAGAATTT GAAGAAGGCC CTGTGGATGT CCAGACTTCC 
CTCCAGGCTA ACACTGAAGC TACTGAAGAA AATGAACATG ATGAACAGGT CCTACAACGT 
GACTTTAAAA AGACAGCAGA AAGCAAAAAT GTCCCATTGG AACGAGAAGC CACTAGTAAA 
AATGACCAAA ATAACTGTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AGATGAGCAA 
CCTTTAAATA GTGCTGCCCA TAAGGAGTCA CCTCCTACTG TTGATTCAAC TCAACAGCCT 
AACCCTTTGC CGTTACGTTT ACCTGAAATG GAACCCTTAG TGCCTAGAGT CAAAGAAGTT 
AAATCTGCTC AGGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAATATAA GTCAAAAGTC TGATGAAGAA 
GATTTTGTAA AAGTTGAAGA TTTACCACTG AAACTGACAA TATATTCAGA GGCAGATCTA 
AGAAAGAAAA TGGTAGAAGA AGAACAGAAA AACCATTTAT CTGGTGAAAT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG AAATTCTGAG ACACTAAAAG AACCTGAAAC GGTGGGAGCC 
CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868964.1
CDS	632..6823
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013475.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013475.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA GGTATGAAAG TGCCAGTATG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CATGAGCAAC TGGAAAAAAT AATAAAATGT 
AATAGGTCTA CAGAAATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCCTC GCAGCTTCTA 
ACTTCAGTAA GGCGCATGGT TTTGACCCTT ACCCAGCAAA ATGATGAGAG CAAAGAGTTT 
GTAAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTC 
AATGAATTGG CTTTCTTTAA GCTTATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAGATGCA AAAGGAAAAT AGAAGCAACT GGAGTGATAC AATCTTGTGC CAAAGAGGCT 
AAAAGGATTC TTGAAGATCA TGGCTCACCT GCTGGAGAGA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCAGACT CAAACATCTG AGGTGTATGA TGGTCCCAAA 
AATGTAAGAT CTGATATTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGG ATGTCCAGTG 
TCTATTAATT TGTCTAAAGC TGAAACTCAG GCTTTAACTA ATTATGGAAG TGGAGAAGAT 
GAAAATGAGG ATGAAGAAAT GGAAGAATTT GAAGAAGGCC CTGTGGATGT CCAGACTTCC 
CTCCAGGCTA ACACTGAAGC TACTGAAGAA AATGAACATG ATGAACAGGT CCTACAACGT 
GACTTTAAAA AGACAGCAGA AAGCAAAAAT GTCCCATTGG AACGAGAAGC CACTAGTAAA 
AATGACCAAA ATAACTGTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AGATGAGCAA 
CCTTTAAATA GTGCTGCCCA TAAGGAGTCA CCTCCTACTG TTGATTCAAC TCAACAGCCT 
AACCCTTTGC CGTTACGTTT ACCTGAAATG GAACCCTTAG TGCCTAGAGT CAAAGAAGTT 
AAATCTGCTC AGGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAATATAA GTCAAAAGTC TGATGAAGAA 
GATTTTGTAA AAGTTGAAGA TTTACCACTG AAACTGACAA TATATTCAGA GGCAGATCTA 
AGAAAGAAAA TGGTAGAAGA AGAACAGAAA AACCATTTAT CTGGTGAAAT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG AAATTCTGAG ACACTAAAAG AACCTGAAAC GGTGGGAGCC 
CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013477.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868966.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X16, mRNA.

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CloneID	OHu98020
Clone ID Related Accession (Same CDS sequence)	NM_001352633.2 , XM_017013477.1
Accession Version	XM_017013477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA TTGTCACTTT TTTTACTCAA 
GGGTATGAAA GTGCCAGTAT GTCTAGCACA TGTGAACCTT GCAAAAGTAG GAACAGACAT 
TCAGCCCAGA CTGAAGAGCC TGTTCAAGCA AAAGTATTCA GCAGAAAGAA TCATGAGCAA 
CTGGAAAAAA TAATAAAATG TAATAGGTCT ACAGAAATAT CTTCAGAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868966.1
CDS	800..6988
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIIVTFFTQGYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013477.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013477.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA TTGTCACTTT TTTTACTCAA 
GGGTATGAAA GTGCCAGTAT GTCTAGCACA TGTGAACCTT GCAAAAGTAG GAACAGACAT 
TCAGCCCAGA CTGAAGAGCC TGTTCAAGCA AAAGTATTCA GCAGAAAGAA TCATGAGCAA 
CTGGAAAAAA TAATAAAATG TAATAGGTCT ACAGAAATAT CTTCAGAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGAGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCGTCCACA TTTTCTTATT GAACTCTTCC ATGAGCTGCA GCTACTAAAC 
ACAGACTACT TGAGACAGAG GGCTTTATAT GCATTGCAGG ACATAGTATC CAGACATATT 
TCTGAGAGCC ATGAAAAAGG AGAAAATGTA AAGTCAGTAA ACTCTGGTAC TTGGATAGCA 
TCAAACTCAG AACTTACTCC TAGTGAGAGC CTTGCTACTA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AAAGAGAAAC CCATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGT 
GTCCTGTCTG TATCATCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACCGTG 
ATTCACTTAG ATCAAGCATT AGCCAGAATG AGAGAATATG AGCGTATGAA GACTGAGGCT 
GAAAGTAACT CAAATATGAG ATGCACCTGC AGGATTATTG AGGATGGAGA TGGTGCTGGT 
GCAGAAACTC CCGTTATTGA AAATCGTAGT TCACAACAAC CTGTAAGTGA AGTTTCTACC 
ATCCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC AATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCCTCGCA GCTTCTAACT 
TCAGTAAGGC GCATGGTTTT GACCCTTACC CAGCAAAATG ATGAGAGCAA AGAGTTTGTA 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTCAAT 
GAATTGGCTT TCTTTAAGCT TATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AGATGCAAAA GGAAAATAGA AGCAACTGGA GTGATACAAT CTTGTGCCAA AGAGGCTAAA 
AGGATTCTTG AAGATCATGG CTCACCTGCT GGAGAGATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCAGACTCAA ACATCTGAGG TGTATGATGG TCCCAAAAAT 
GTAAGATCTG ATATTTCTGA TCAAGAGGAA GATGAAGAAA GTGAAGGATG TCCAGTGTCT 
ATTAATTTGT CTAAAGCTGA AACTCAGGCT TTAACTAATT ATGGAAGTGG AGAAGATGAA 
AATGAGGATG AAGAAATGGA AGAATTTGAA GAAGGCCCTG TGGATGTCCA GACTTCCCTC 
CAGGCTAACA CTGAAGCTAC TGAAGAAAAT GAACATGATG AACAGGTCCT ACAACGTGAC 
TTTAAAAAGA CAGCAGAAAG CAAAAATGTC CCATTGGAAC GAGAAGCCAC TAGTAAAAAT 
GACCAAAATA ACTGTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAGA TGAGCAACCT 
TTAAATAGTG CTGCCCATAA GGAGTCACCT CCTACTGTTG ATTCAACTCA ACAGCCTAAC 
CCTTTGCCGT TACGTTTACC TGAAATGGAA CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA 
TCTGCTCAGG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AATATAAGTC AAAAGTCTGA TGAAGAAGAT 
TTTGTAAAAG TTGAAGATTT ACCACTGAAA CTGACAATAT ATTCAGAGGC AGATCTAAGA 
AAGAAAATGG TAGAAGAAGA ACAGAAAAAC CATTTATCTG GTGAAATATG TGAAATGCAG 
ACCGAAGAAT TAGCTGGAAA TTCTGAGACA CTAAAAGAAC CTGAAACGGT GGGAGCCCAG 
AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013480.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868969.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X13, mRNA.

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CloneID	OHu98022
Clone ID Related Accession (Same CDS sequence)	XM_017013480.1 , XM_017013481.1
Accession Version	XM_017013480.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGATTTT TCTCAGGAGC ACATGGATGA AGTATGCTCC 
TCGCAGCTTC TAACTTCAGT AAGGCGCATG GTTTTGACCC TTACCCAGCA AAATGATGAG 
AGCAAAGAGT TTGTAAAGTT CTTTCATAAA CAACTTGGAA GTATATTACA GGATTCACTG 
GCAAAATTTG CTGGCAGAAA ACTGAAAGAC TGTGGAGAAG ATCTTCTTGT AGAGATATCT 
GAAGTGTTGT TCAATGAATT GGCTTTCTTT AAGCTTATGC AAGATTTGGA TAATAATAGT 
ATAACTGTTA AACAGAGATG CAAAAGGAAA ATAGAAGCAA CTGGAGTGAT ACAATCTTGT 
GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT CATGGCTCAC CTGCTGGAGA GATTGATGAT 
GAAGACAAAG ACAAGGATGA AACTGAAACA GTTAAGCAGA CTCAAACATC TGAGGTGTAT 
GATGGTCCCA AAAATGTAAG ATCTGATATT TCTGATCAAG AGGAAGATGA AGAAAGTGAA 
GGATGTCCAG TGTCTATTAA TTTGTCTAAA GCTGAAACTC AGGCTTTAAC TAATTATGGA 
AGTGGAGAAG ATGAAAATGA GGATGAAGAA ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT 
GTCCAGACTT CCCTCCAGGC TAACACTGAA GCTACTGAAG AAAATGAACA TGATGAACAG 
GTCCTACAAC GTGACTTTAA AAAGACAGCA GAAAGCAAAA ATGTCCCATT GGAACGAGAA 
GCCACTAGTA AAAATGACCA AAATAACTGT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAGATGAGC AACCTTTAAA TAGTGCTGCC CATAAGGAGT CACCTCCTAC TGTTGATTCA 
ACTCAACAGC CTAACCCTTT GCCGTTACGT TTACCTGAAA TGGAACCCTT AGTGCCTAGA 
GTCAAAGAAG TTAAATCTGC TCAGGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAATAT AAGTCAAAAG 
TCTGATGAAG AAGATTTTGT AAAAGTTGAA GATTTACCAC TGAAACTGAC AATATATTCA 
GAGGCAGATC TAAGAAAGAA AATGGTAGAA GAAGAACAGA AAAACCATTT ATCTGGTGAA 
ATATGTGAAA TGCAGACCGA AGAATTAGCT GGAAATTCTG AGACACTAAA AGAACCTGAA 
ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868969.1
CDS	496..6639
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013480.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013480.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGATTTT TCTCAGGAGC ACATGGATGA AGTATGCTCC 
TCGCAGCTTC TAACTTCAGT AAGGCGCATG GTTTTGACCC TTACCCAGCA AAATGATGAG 
AGCAAAGAGT TTGTAAAGTT CTTTCATAAA CAACTTGGAA GTATATTACA GGATTCACTG 
GCAAAATTTG CTGGCAGAAA ACTGAAAGAC TGTGGAGAAG ATCTTCTTGT AGAGATATCT 
GAAGTGTTGT TCAATGAATT GGCTTTCTTT AAGCTTATGC AAGATTTGGA TAATAATAGT 
ATAACTGTTA AACAGAGATG CAAAAGGAAA ATAGAAGCAA CTGGAGTGAT ACAATCTTGT 
GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT CATGGCTCAC CTGCTGGAGA GATTGATGAT 
GAAGACAAAG ACAAGGATGA AACTGAAACA GTTAAGCAGA CTCAAACATC TGAGGTGTAT 
GATGGTCCCA AAAATGTAAG ATCTGATATT TCTGATCAAG AGGAAGATGA AGAAAGTGAA 
GGATGTCCAG TGTCTATTAA TTTGTCTAAA GCTGAAACTC AGGCTTTAAC TAATTATGGA 
AGTGGAGAAG ATGAAAATGA GGATGAAGAA ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT 
GTCCAGACTT CCCTCCAGGC TAACACTGAA GCTACTGAAG AAAATGAACA TGATGAACAG 
GTCCTACAAC GTGACTTTAA AAAGACAGCA GAAAGCAAAA ATGTCCCATT GGAACGAGAA 
GCCACTAGTA AAAATGACCA AAATAACTGT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAGATGAGC AACCTTTAAA TAGTGCTGCC CATAAGGAGT CACCTCCTAC TGTTGATTCA 
ACTCAACAGC CTAACCCTTT GCCGTTACGT TTACCTGAAA TGGAACCCTT AGTGCCTAGA 
GTCAAAGAAG TTAAATCTGC TCAGGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAATAT AAGTCAAAAG 
TCTGATGAAG AAGATTTTGT AAAAGTTGAA GATTTACCAC TGAAACTGAC AATATATTCA 
GAGGCAGATC TAAGAAAGAA AATGGTAGAA GAAGAACAGA AAAACCATTT ATCTGGTGAA 
ATATGTGAAA TGCAGACCGA AGAATTAGCT GGAAATTCTG AGACACTAAA AGAACCTGAA 
ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013481.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868970.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X14, mRNA.

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CloneID	OHu98022
Clone ID Related Accession (Same CDS sequence)	XM_017013480.1 , XM_017013481.1
Accession Version	XM_017013481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGATTTT TCTCAGGAGC ACATGGATGA AGTATGCTCC 
TCGCAGCTTC TAACTTCAGT AAGGCGCATG GTTTTGACCC TTACCCAGCA AAATGATGAG 
AGCAAAGAGT TTGTAAAGTT CTTTCATAAA CAACTTGGAA GTATATTACA GGATTCACTG 
GCAAAATTTG CTGGCAGAAA ACTGAAAGAC TGTGGAGAAG ATCTTCTTGT AGAGATATCT 
GAAGTGTTGT TCAATGAATT GGCTTTCTTT AAGCTTATGC AAGATTTGGA TAATAATAGT 
ATAACTGTTA AACAGAGATG CAAAAGGAAA ATAGAAGCAA CTGGAGTGAT ACAATCTTGT 
GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT CATGGCTCAC CTGCTGGAGA GATTGATGAT 
GAAGACAAAG ACAAGGATGA AACTGAAACA GTTAAGCAGA CTCAAACATC TGAGGTGTAT 
GATGGTCCCA AAAATGTAAG ATCTGATATT TCTGATCAAG AGGAAGATGA AGAAAGTGAA 
GGATGTCCAG TGTCTATTAA TTTGTCTAAA GCTGAAACTC AGGCTTTAAC TAATTATGGA 
AGTGGAGAAG ATGAAAATGA GGATGAAGAA ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT 
GTCCAGACTT CCCTCCAGGC TAACACTGAA GCTACTGAAG AAAATGAACA TGATGAACAG 
GTCCTACAAC GTGACTTTAA AAAGACAGCA GAAAGCAAAA ATGTCCCATT GGAACGAGAA 
GCCACTAGTA AAAATGACCA AAATAACTGT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAGATGAGC AACCTTTAAA TAGTGCTGCC CATAAGGAGT CACCTCCTAC TGTTGATTCA 
ACTCAACAGC CTAACCCTTT GCCGTTACGT TTACCTGAAA TGGAACCCTT AGTGCCTAGA 
GTCAAAGAAG TTAAATCTGC TCAGGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAATAT AAGTCAAAAG 
TCTGATGAAG AAGATTTTGT AAAAGTTGAA GATTTACCAC TGAAACTGAC AATATATTCA 
GAGGCAGATC TAAGAAAGAA AATGGTAGAA GAAGAACAGA AAAACCATTT ATCTGGTGAA 
ATATGTGAAA TGCAGACCGA AGAATTAGCT GGAAATTCTG AGACACTAAA AGAACCTGAA 
ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868970.1
CDS	799..6942
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013481.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013481.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC GCCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGAGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCGTCCACAT 
TTTCTTATTG AACTCTTCCA TGAGCTGCAG CTACTAAACA CAGACTACTT GAGACAGAGG 
GCTTTATATG CATTGCAGGA CATAGTATCC AGACATATTT CTGAGAGCCA TGAAAAAGGA 
GAAAATGTAA AGTCAGTAAA CTCTGGTACT TGGATAGCAT CAAACTCAGA ACTTACTCCT 
AGTGAGAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA AAGAGAAACC 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TCCTGTCTGT ATCATCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACCGTGA TTCACTTAGA TCAAGCATTA 
GCCAGAATGA GAGAATATGA GCGTATGAAG ACTGAGGCTG AAAGTAACTC AAATATGAGA 
TGCACCTGCA GGATTATTGA GGATGGAGAT GGTGCTGGTG CAGGTACTAC AGTTAATAAT 
TTAGAAGAAA CTCCCGTTAT TGAAAATCGT AGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACCATCCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCAATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGATTTT TCTCAGGAGC ACATGGATGA AGTATGCTCC 
TCGCAGCTTC TAACTTCAGT AAGGCGCATG GTTTTGACCC TTACCCAGCA AAATGATGAG 
AGCAAAGAGT TTGTAAAGTT CTTTCATAAA CAACTTGGAA GTATATTACA GGATTCACTG 
GCAAAATTTG CTGGCAGAAA ACTGAAAGAC TGTGGAGAAG ATCTTCTTGT AGAGATATCT 
GAAGTGTTGT TCAATGAATT GGCTTTCTTT AAGCTTATGC AAGATTTGGA TAATAATAGT 
ATAACTGTTA AACAGAGATG CAAAAGGAAA ATAGAAGCAA CTGGAGTGAT ACAATCTTGT 
GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT CATGGCTCAC CTGCTGGAGA GATTGATGAT 
GAAGACAAAG ACAAGGATGA AACTGAAACA GTTAAGCAGA CTCAAACATC TGAGGTGTAT 
GATGGTCCCA AAAATGTAAG ATCTGATATT TCTGATCAAG AGGAAGATGA AGAAAGTGAA 
GGATGTCCAG TGTCTATTAA TTTGTCTAAA GCTGAAACTC AGGCTTTAAC TAATTATGGA 
AGTGGAGAAG ATGAAAATGA GGATGAAGAA ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT 
GTCCAGACTT CCCTCCAGGC TAACACTGAA GCTACTGAAG AAAATGAACA TGATGAACAG 
GTCCTACAAC GTGACTTTAA AAAGACAGCA GAAAGCAAAA ATGTCCCATT GGAACGAGAA 
GCCACTAGTA AAAATGACCA AAATAACTGT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAGATGAGC AACCTTTAAA TAGTGCTGCC CATAAGGAGT CACCTCCTAC TGTTGATTCA 
ACTCAACAGC CTAACCCTTT GCCGTTACGT TTACCTGAAA TGGAACCCTT AGTGCCTAGA 
GTCAAAGAAG TTAAATCTGC TCAGGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAATAT AAGTCAAAAG 
TCTGATGAAG AAGATTTTGT AAAAGTTGAA GATTTACCAC TGAAACTGAC AATATATTCA 
GAGGCAGATC TAAGAAAGAA AATGGTAGAA GAAGAACAGA AAAACCATTT ATCTGGTGAA 
ATATGTGAAA TGCAGACCGA AGAATTAGCT GGAAATTCTG AGACACTAAA AGAACCTGAA 
ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013482.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868971.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X21, mRNA.

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CloneID	OHu98023
Clone ID Related Accession (Same CDS sequence)	XM_017013482.1 , NM_001352634.2
Accession Version	XM_017013482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAT TTTTCTCAGG AGCACATGGA TGAAGTATGC 
TCCTCGCAGC TTCTAACTTC AGTAAGGCGC ATGGTTTTGA CCCTTACCCA GCAAAATGAT 
GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT AAACAACTTG GAAGTATATT ACAGGATTCA 
CTGGCAAAAT TTGCTGGCAG AAAACTGAAA GACTGTGGAG AAGATCTTCT TGTAGAGATA 
TCTGAAGTGT TGTTCAATGA ATTGGCTTTC TTTAAGCTTA TGCAAGATTT GGATAATAAT 
AGTATAACTG TTAAACAGAG ATGCAAAAGG AAAATAGAAG CAACTGGAGT GATACAATCT 
TGTGCCAAAG AGGCTAAAAG GATTCTTGAA GATCATGGCT CACCTGCTGG AGAGATTGAT 
GATGAAGACA AAGACAAGGA TGAAACTGAA ACAGTTAAGC AGACTCAAAC ATCTGAGGTG 
TATGATGGTC CCAAAAATGT AAGATCTGAT ATTTCTGATC AAGAGGAAGA TGAAGAAAGT 
GAAGGATGTC CAGTGTCTAT TAATTTGTCT AAAGCTGAAA CTCAGGCTTT AACTAATTAT 
GGAAGTGGAG AAGATGAAAA TGAGGATGAA GAAATGGAAG AATTTGAAGA AGGCCCTGTG 
GATGTCCAGA CTTCCCTCCA GGCTAACACT GAAGCTACTG AAGAAAATGA ACATGATGAA 
CAGGTCCTAC AACGTGACTT TAAAAAGACA GCAGAAAGCA AAAATGTCCC ATTGGAACGA 
GAAGCCACTA GTAAAAATGA CCAAAATAAC TGTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAGATG AGCAACCTTT AAATAGTGCT GCCCATAAGG AGTCACCTCC TACTGTTGAT 
TCAACTCAAC AGCCTAACCC TTTGCCGTTA CGTTTACCTG AAATGGAACC CTTAGTGCCT 
AGAGTCAAAG AAGTTAAATC TGCTCAGGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA TATAAGTCAA 
AAGTCTGATG AAGAAGATTT TGTAAAAGTT GAAGATTTAC CACTGAAACT GACAATATAT 
TCAGAGGCAG ATCTAAGAAA GAAAATGGTA GAAGAAGAAC AGAAAAACCA TTTATCTGGT 
GAAATATGTG AAATGCAGAC CGAAGAATTA GCTGGAAATT CTGAGACACT AAAAGAACCT 
GAAACGGTGG GAGCCCAGAG TATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868971.1
CDS	517..6603
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013482.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013482.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAT TTTTCTCAGG AGCACATGGA TGAAGTATGC 
TCCTCGCAGC TTCTAACTTC AGTAAGGCGC ATGGTTTTGA CCCTTACCCA GCAAAATGAT 
GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT AAACAACTTG GAAGTATATT ACAGGATTCA 
CTGGCAAAAT TTGCTGGCAG AAAACTGAAA GACTGTGGAG AAGATCTTCT TGTAGAGATA 
TCTGAAGTGT TGTTCAATGA ATTGGCTTTC TTTAAGCTTA TGCAAGATTT GGATAATAAT 
AGTATAACTG TTAAACAGAG ATGCAAAAGG AAAATAGAAG CAACTGGAGT GATACAATCT 
TGTGCCAAAG AGGCTAAAAG GATTCTTGAA GATCATGGCT CACCTGCTGG AGAGATTGAT 
GATGAAGACA AAGACAAGGA TGAAACTGAA ACAGTTAAGC AGACTCAAAC ATCTGAGGTG 
TATGATGGTC CCAAAAATGT AAGATCTGAT ATTTCTGATC AAGAGGAAGA TGAAGAAAGT 
GAAGGATGTC CAGTGTCTAT TAATTTGTCT AAAGCTGAAA CTCAGGCTTT AACTAATTAT 
GGAAGTGGAG AAGATGAAAA TGAGGATGAA GAAATGGAAG AATTTGAAGA AGGCCCTGTG 
GATGTCCAGA CTTCCCTCCA GGCTAACACT GAAGCTACTG AAGAAAATGA ACATGATGAA 
CAGGTCCTAC AACGTGACTT TAAAAAGACA GCAGAAAGCA AAAATGTCCC ATTGGAACGA 
GAAGCCACTA GTAAAAATGA CCAAAATAAC TGTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAGATG AGCAACCTTT AAATAGTGCT GCCCATAAGG AGTCACCTCC TACTGTTGAT 
TCAACTCAAC AGCCTAACCC TTTGCCGTTA CGTTTACCTG AAATGGAACC CTTAGTGCCT 
AGAGTCAAAG AAGTTAAATC TGCTCAGGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA TATAAGTCAA 
AAGTCTGATG AAGAAGATTT TGTAAAAGTT GAAGATTTAC CACTGAAACT GACAATATAT 
TCAGAGGCAG ATCTAAGAAA GAAAATGGTA GAAGAAGAAC AGAAAAACCA TTTATCTGGT 
GAAATATGTG AAATGCAGAC CGAAGAATTA GCTGGAAATT CTGAGACACT AAAAGAACCT 
GAAACGGTGG GAGCCCAGAG TATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013483.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868972.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X22, mRNA.

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CloneID	OHu98024
Clone ID Related Accession (Same CDS sequence)	XM_017013483.1 , NM_001352652.2 , XM_017013484.1 , NM_001352653.2
Accession Version	XM_017013483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X17
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC CTCGCAGCTT 
CTAACTTCAG TAAGGCGCAT GGTTTTGACC CTTACCCAGC AAAATGATGA GAGCAAAGAG 
TTTGTAAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTCAATGAAT TGGCTTTCTT TAAGCTTATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAGAT GCAAAAGGAA AATAGAAGCA ACTGGAGTGA TACAATCTTG TGCCAAAGAG 
GCTAAAAGGA TTCTTGAAGA TCATGGCTCA CCTGCTGGAG AGATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCAG ACTCAAACAT CTGAGGTGTA TGATGGTCCC 
AAAAATGTAA GATCTGATAT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGGATGTCCA 
GTGTCTATTA ATTTGTCTAA AGCTGAAACT CAGGCTTTAA CTAATTATGG AAGTGGAGAA 
GATGAAAATG AGGATGAAGA AATGGAAGAA TTTGAAGAAG GCCCTGTGGA TGTCCAGACT 
TCCCTCCAGG CTAACACTGA AGCTACTGAA GAAAATGAAC ATGATGAACA GGTCCTACAA 
CGTGACTTTA AAAAGACAGC AGAAAGCAAA AATGTCCCAT TGGAACGAGA AGCCACTAGT 
AAAAATGACC AAAATAACTG TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAGATGAG 
CAACCTTTAA ATAGTGCTGC CCATAAGGAG TCACCTCCTA CTGTTGATTC AACTCAACAG 
CCTAACCCTT TGCCGTTACG TTTACCTGAA ATGGAACCCT TAGTGCCTAG AGTCAAAGAA 
GTTAAATCTG CTCAGGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAATA TAAGTCAAAA GTCTGATGAA 
GAAGATTTTG TAAAAGTTGA AGATTTACCA CTGAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGAAAGA AAATGGTAGA AGAAGAACAG AAAAACCATT TATCTGGTGA AATATGTGAA 
ATGCAGACCG AAGAATTAGC TGGAAATTCT GAGACACTAA AAGAACCTGA AACGGTGGGA 
GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868972.1
CDS	798..6872
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013483.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013483.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC CTCGCAGCTT 
CTAACTTCAG TAAGGCGCAT GGTTTTGACC CTTACCCAGC AAAATGATGA GAGCAAAGAG 
TTTGTAAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTCAATGAAT TGGCTTTCTT TAAGCTTATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAGAT GCAAAAGGAA AATAGAAGCA ACTGGAGTGA TACAATCTTG TGCCAAAGAG 
GCTAAAAGGA TTCTTGAAGA TCATGGCTCA CCTGCTGGAG AGATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCAG ACTCAAACAT CTGAGGTGTA TGATGGTCCC 
AAAAATGTAA GATCTGATAT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGGATGTCCA 
GTGTCTATTA ATTTGTCTAA AGCTGAAACT CAGGCTTTAA CTAATTATGG AAGTGGAGAA 
GATGAAAATG AGGATGAAGA AATGGAAGAA TTTGAAGAAG GCCCTGTGGA TGTCCAGACT 
TCCCTCCAGG CTAACACTGA AGCTACTGAA GAAAATGAAC ATGATGAACA GGTCCTACAA 
CGTGACTTTA AAAAGACAGC AGAAAGCAAA AATGTCCCAT TGGAACGAGA AGCCACTAGT 
AAAAATGACC AAAATAACTG TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAGATGAG 
CAACCTTTAA ATAGTGCTGC CCATAAGGAG TCACCTCCTA CTGTTGATTC AACTCAACAG 
CCTAACCCTT TGCCGTTACG TTTACCTGAA ATGGAACCCT TAGTGCCTAG AGTCAAAGAA 
GTTAAATCTG CTCAGGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAATA TAAGTCAAAA GTCTGATGAA 
GAAGATTTTG TAAAAGTTGA AGATTTACCA CTGAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGAAAGA AAATGGTAGA AGAAGAACAG AAAAACCATT TATCTGGTGA AATATGTGAA 
ATGCAGACCG AAGAATTAGC TGGAAATTCT GAGACACTAA AAGAACCTGA AACGGTGGGA 
GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013484.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868973.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X23, mRNA.

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CloneID	OHu98024
Clone ID Related Accession (Same CDS sequence)	XM_017013483.1 , NM_001352652.2 , XM_017013484.1 , NM_001352653.2
Accession Version	XM_017013484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X17
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC CTCGCAGCTT 
CTAACTTCAG TAAGGCGCAT GGTTTTGACC CTTACCCAGC AAAATGATGA GAGCAAAGAG 
TTTGTAAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTCAATGAAT TGGCTTTCTT TAAGCTTATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAGAT GCAAAAGGAA AATAGAAGCA ACTGGAGTGA TACAATCTTG TGCCAAAGAG 
GCTAAAAGGA TTCTTGAAGA TCATGGCTCA CCTGCTGGAG AGATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCAG ACTCAAACAT CTGAGGTGTA TGATGGTCCC 
AAAAATGTAA GATCTGATAT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGGATGTCCA 
GTGTCTATTA ATTTGTCTAA AGCTGAAACT CAGGCTTTAA CTAATTATGG AAGTGGAGAA 
GATGAAAATG AGGATGAAGA AATGGAAGAA TTTGAAGAAG GCCCTGTGGA TGTCCAGACT 
TCCCTCCAGG CTAACACTGA AGCTACTGAA GAAAATGAAC ATGATGAACA GGTCCTACAA 
CGTGACTTTA AAAAGACAGC AGAAAGCAAA AATGTCCCAT TGGAACGAGA AGCCACTAGT 
AAAAATGACC AAAATAACTG TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAGATGAG 
CAACCTTTAA ATAGTGCTGC CCATAAGGAG TCACCTCCTA CTGTTGATTC AACTCAACAG 
CCTAACCCTT TGCCGTTACG TTTACCTGAA ATGGAACCCT TAGTGCCTAG AGTCAAAGAA 
GTTAAATCTG CTCAGGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAATA TAAGTCAAAA GTCTGATGAA 
GAAGATTTTG TAAAAGTTGA AGATTTACCA CTGAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGAAAGA AAATGGTAGA AGAAGAACAG AAAAACCATT TATCTGGTGA AATATGTGAA 
ATGCAGACCG AAGAATTAGC TGGAAATTCT GAGACACTAA AAGAACCTGA AACGGTGGGA 
GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868973.1
CDS	224..6298
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013484.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013484.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC CTCGCAGCTT 
CTAACTTCAG TAAGGCGCAT GGTTTTGACC CTTACCCAGC AAAATGATGA GAGCAAAGAG 
TTTGTAAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTCAATGAAT TGGCTTTCTT TAAGCTTATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAGAT GCAAAAGGAA AATAGAAGCA ACTGGAGTGA TACAATCTTG TGCCAAAGAG 
GCTAAAAGGA TTCTTGAAGA TCATGGCTCA CCTGCTGGAG AGATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCAG ACTCAAACAT CTGAGGTGTA TGATGGTCCC 
AAAAATGTAA GATCTGATAT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGGATGTCCA 
GTGTCTATTA ATTTGTCTAA AGCTGAAACT CAGGCTTTAA CTAATTATGG AAGTGGAGAA 
GATGAAAATG AGGATGAAGA AATGGAAGAA TTTGAAGAAG GCCCTGTGGA TGTCCAGACT 
TCCCTCCAGG CTAACACTGA AGCTACTGAA GAAAATGAAC ATGATGAACA GGTCCTACAA 
CGTGACTTTA AAAAGACAGC AGAAAGCAAA AATGTCCCAT TGGAACGAGA AGCCACTAGT 
AAAAATGACC AAAATAACTG TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAGATGAG 
CAACCTTTAA ATAGTGCTGC CCATAAGGAG TCACCTCCTA CTGTTGATTC AACTCAACAG 
CCTAACCCTT TGCCGTTACG TTTACCTGAA ATGGAACCCT TAGTGCCTAG AGTCAAAGAA 
GTTAAATCTG CTCAGGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAATA TAAGTCAAAA GTCTGATGAA 
GAAGATTTTG TAAAAGTTGA AGATTTACCA CTGAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGAAAGA AAATGGTAGA AGAAGAACAG AAAAACCATT TATCTGGTGA AATATGTGAA 
ATGCAGACCG AAGAATTAGC TGGAAATTCT GAGACACTAA AAGAACCTGA AACGGTGGGA 
GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_024447169.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302937.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X15, mRNA.

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CloneID	OHu98025
Clone ID Related Accession (Same CDS sequence)	XM_024447169.1 , XM_017013485.1
Accession Version	XM_024447169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAGGTA TGAAAGTGCC 
AGTATGTCTA GCACATGTGA ACCTTGCAAA AGTAGGAACA GACATTCAGC CCAGACTGAA 
GAGCCTGTTC AAGCAAAAGT ATTCAGCAGA AAGAATCATG AGCAACTGGA AAAAATAATA 
AAATGTAATA GGTCTACAGA AATATCTTCA GAAACTGGGA GTGATTTTTC CATGTTTGAA 
GCTTTGCGAG ATACTATTTA TTCTGAAGTA GCTACATTAA TTTCTCAAAA TGAATCTCGT 
CCACATTTTC TTATTGAACT CTTCCATGAG CTGCAGCTAC TAAACACAGA CTACTTGAGA 
CAGAGGGCTT TATATGCATT GCAGGACATA GTATCCAGAC ATATTTCTGA GAGCCATGAA 
AAAGGAGAAA ATGTAAAGTC AGTAAACTCT GGTACTTGGA TAGCATCAAA CTCAGAACTT 
ACTCCTAGTG AGAGCCTTGC TACTACTGAT GATGAAACTT TTGAGAAGAA CTTTGAAAGA 
GAAACCCATA AAATAAGTGA GCAAAATGAT GCTGATAATG CTAGTGTCCT GTCTGTATCA 
TCAAATTTTG AGCCTTTTGC AACAGATGAT CTAGGTAACA CCGTGATTCA CTTAGATCAA 
GCATTAGCCA GAATGAGAGA ATATGAGCGT ATGAAGACTG AGGCTGAAAG TAACTCAAAT 
ATGAGATGCA CCTGCAGGAT TATTGAGGAT GGAGATGGTG CTGGTGCAGA AACTCCCGTT 
ATTGAAAATC GTAGTTCACA ACAACCTGTA AGTGAAGTTT CTACCATCCC ATGTCCTAGA 
ATTGATACTC AGCAGCTGGA CCGGCAAATT AAAGCAATTA TGAAAGAAGT CATTCCTTTT 
TTGAAGGAGC ACATGGATGA AGTATGCTCC TCGCAGCTTC TAACTTCAGT AAGGCGCATG 
GTTTTGACCC TTACCCAGCA AAATGATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TCAATGAATT GGCTTTCTTT 
AAGCTTATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCAA CTGGAGTGAT ACAATCTTGT GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT 
CATGGCTCAC CTGCTGGAGA GATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCAGA CTCAAACATC TGAGGTGTAT GATGGTCCCA AAAATGTAAG ATCTGATATT 
TCTGATCAAG AGGAAGATGA AGAAAGTGAA GGATGTCCAG TGTCTATTAA TTTGTCTAAA 
GCTGAAACTC AGGCTTTAAC TAATTATGGA AGTGGAGAAG ATGAAAATGA GGATGAAGAA 
ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT GTCCAGACTT CCCTCCAGGC TAACACTGAA 
GCTACTGAAG AAAATGAACA TGATGAACAG GTCCTACAAC GTGACTTTAA AAAGACAGCA 
GAAAGCAAAA ATGTCCCATT GGAACGAGAA GCCACTAGTA AAAATGACCA AAATAACTGT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAGATGAGC AACCTTTAAA TAGTGCTGCC 
CATAAGGAGT CACCTCCTAC TGTTGATTCA ACTCAACAGC CTAACCCTTT GCCGTTACGT 
TTACCTGAAA TGGAACCCTT AGTGCCTAGA GTCAAAGAAG TTAAATCTGC TCAGGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAATAT AAGTCAAAAG TCTGATGAAG AAGATTTTGT AAAAGTTGAA 
GATTTACCAC TGAAACTGAC AATATATTCA GAGGCAGATC TAAGAAAGAA AATGGTAGAA 
GAAGAACAGA AAAACCATTT ATCTGGTGAA ATATGTGAAA TGCAGACCGA AGAATTAGCT 
GGAAATTCTG AGACACTAAA AGAACCTGAA ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302937.1
CDS	496..6549
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_024447169.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447169.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAGGTA TGAAAGTGCC 
AGTATGTCTA GCACATGTGA ACCTTGCAAA AGTAGGAACA GACATTCAGC CCAGACTGAA 
GAGCCTGTTC AAGCAAAAGT ATTCAGCAGA AAGAATCATG AGCAACTGGA AAAAATAATA 
AAATGTAATA GGTCTACAGA AATATCTTCA GAAACTGGGA GTGATTTTTC CATGTTTGAA 
GCTTTGCGAG ATACTATTTA TTCTGAAGTA GCTACATTAA TTTCTCAAAA TGAATCTCGT 
CCACATTTTC TTATTGAACT CTTCCATGAG CTGCAGCTAC TAAACACAGA CTACTTGAGA 
CAGAGGGCTT TATATGCATT GCAGGACATA GTATCCAGAC ATATTTCTGA GAGCCATGAA 
AAAGGAGAAA ATGTAAAGTC AGTAAACTCT GGTACTTGGA TAGCATCAAA CTCAGAACTT 
ACTCCTAGTG AGAGCCTTGC TACTACTGAT GATGAAACTT TTGAGAAGAA CTTTGAAAGA 
GAAACCCATA AAATAAGTGA GCAAAATGAT GCTGATAATG CTAGTGTCCT GTCTGTATCA 
TCAAATTTTG AGCCTTTTGC AACAGATGAT CTAGGTAACA CCGTGATTCA CTTAGATCAA 
GCATTAGCCA GAATGAGAGA ATATGAGCGT ATGAAGACTG AGGCTGAAAG TAACTCAAAT 
ATGAGATGCA CCTGCAGGAT TATTGAGGAT GGAGATGGTG CTGGTGCAGA AACTCCCGTT 
ATTGAAAATC GTAGTTCACA ACAACCTGTA AGTGAAGTTT CTACCATCCC ATGTCCTAGA 
ATTGATACTC AGCAGCTGGA CCGGCAAATT AAAGCAATTA TGAAAGAAGT CATTCCTTTT 
TTGAAGGAGC ACATGGATGA AGTATGCTCC TCGCAGCTTC TAACTTCAGT AAGGCGCATG 
GTTTTGACCC TTACCCAGCA AAATGATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TCAATGAATT GGCTTTCTTT 
AAGCTTATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCAA CTGGAGTGAT ACAATCTTGT GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT 
CATGGCTCAC CTGCTGGAGA GATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCAGA CTCAAACATC TGAGGTGTAT GATGGTCCCA AAAATGTAAG ATCTGATATT 
TCTGATCAAG AGGAAGATGA AGAAAGTGAA GGATGTCCAG TGTCTATTAA TTTGTCTAAA 
GCTGAAACTC AGGCTTTAAC TAATTATGGA AGTGGAGAAG ATGAAAATGA GGATGAAGAA 
ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT GTCCAGACTT CCCTCCAGGC TAACACTGAA 
GCTACTGAAG AAAATGAACA TGATGAACAG GTCCTACAAC GTGACTTTAA AAAGACAGCA 
GAAAGCAAAA ATGTCCCATT GGAACGAGAA GCCACTAGTA AAAATGACCA AAATAACTGT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAGATGAGC AACCTTTAAA TAGTGCTGCC 
CATAAGGAGT CACCTCCTAC TGTTGATTCA ACTCAACAGC CTAACCCTTT GCCGTTACGT 
TTACCTGAAA TGGAACCCTT AGTGCCTAGA GTCAAAGAAG TTAAATCTGC TCAGGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAATAT AAGTCAAAAG TCTGATGAAG AAGATTTTGT AAAAGTTGAA 
GATTTACCAC TGAAACTGAC AATATATTCA GAGGCAGATC TAAGAAAGAA AATGGTAGAA 
GAAGAACAGA AAAACCATTT ATCTGGTGAA ATATGTGAAA TGCAGACCGA AGAATTAGCT 
GGAAATTCTG AGACACTAAA AGAACCTGAA ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013485.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868974.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X24, mRNA.

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CloneID	OHu98025
Clone ID Related Accession (Same CDS sequence)	XM_024447169.1 , XM_017013485.1
Accession Version	XM_017013485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X18
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAGGTA TGAAAGTGCC 
AGTATGTCTA GCACATGTGA ACCTTGCAAA AGTAGGAACA GACATTCAGC CCAGACTGAA 
GAGCCTGTTC AAGCAAAAGT ATTCAGCAGA AAGAATCATG AGCAACTGGA AAAAATAATA 
AAATGTAATA GGTCTACAGA AATATCTTCA GAAACTGGGA GTGATTTTTC CATGTTTGAA 
GCTTTGCGAG ATACTATTTA TTCTGAAGTA GCTACATTAA TTTCTCAAAA TGAATCTCGT 
CCACATTTTC TTATTGAACT CTTCCATGAG CTGCAGCTAC TAAACACAGA CTACTTGAGA 
CAGAGGGCTT TATATGCATT GCAGGACATA GTATCCAGAC ATATTTCTGA GAGCCATGAA 
AAAGGAGAAA ATGTAAAGTC AGTAAACTCT GGTACTTGGA TAGCATCAAA CTCAGAACTT 
ACTCCTAGTG AGAGCCTTGC TACTACTGAT GATGAAACTT TTGAGAAGAA CTTTGAAAGA 
GAAACCCATA AAATAAGTGA GCAAAATGAT GCTGATAATG CTAGTGTCCT GTCTGTATCA 
TCAAATTTTG AGCCTTTTGC AACAGATGAT CTAGGTAACA CCGTGATTCA CTTAGATCAA 
GCATTAGCCA GAATGAGAGA ATATGAGCGT ATGAAGACTG AGGCTGAAAG TAACTCAAAT 
ATGAGATGCA CCTGCAGGAT TATTGAGGAT GGAGATGGTG CTGGTGCAGA AACTCCCGTT 
ATTGAAAATC GTAGTTCACA ACAACCTGTA AGTGAAGTTT CTACCATCCC ATGTCCTAGA 
ATTGATACTC AGCAGCTGGA CCGGCAAATT AAAGCAATTA TGAAAGAAGT CATTCCTTTT 
TTGAAGGAGC ACATGGATGA AGTATGCTCC TCGCAGCTTC TAACTTCAGT AAGGCGCATG 
GTTTTGACCC TTACCCAGCA AAATGATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TCAATGAATT GGCTTTCTTT 
AAGCTTATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCAA CTGGAGTGAT ACAATCTTGT GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT 
CATGGCTCAC CTGCTGGAGA GATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCAGA CTCAAACATC TGAGGTGTAT GATGGTCCCA AAAATGTAAG ATCTGATATT 
TCTGATCAAG AGGAAGATGA AGAAAGTGAA GGATGTCCAG TGTCTATTAA TTTGTCTAAA 
GCTGAAACTC AGGCTTTAAC TAATTATGGA AGTGGAGAAG ATGAAAATGA GGATGAAGAA 
ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT GTCCAGACTT CCCTCCAGGC TAACACTGAA 
GCTACTGAAG AAAATGAACA TGATGAACAG GTCCTACAAC GTGACTTTAA AAAGACAGCA 
GAAAGCAAAA ATGTCCCATT GGAACGAGAA GCCACTAGTA AAAATGACCA AAATAACTGT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAGATGAGC AACCTTTAAA TAGTGCTGCC 
CATAAGGAGT CACCTCCTAC TGTTGATTCA ACTCAACAGC CTAACCCTTT GCCGTTACGT 
TTACCTGAAA TGGAACCCTT AGTGCCTAGA GTCAAAGAAG TTAAATCTGC TCAGGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAATAT AAGTCAAAAG TCTGATGAAG AAGATTTTGT AAAAGTTGAA 
GATTTACCAC TGAAACTGAC AATATATTCA GAGGCAGATC TAAGAAAGAA AATGGTAGAA 
GAAGAACAGA AAAACCATTT ATCTGGTGAA ATATGTGAAA TGCAGACCGA AGAATTAGCT 
GGAAATTCTG AGACACTAAA AGAACCTGAA ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868974.1
CDS	799..6852
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013485.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013485.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCAGGTA TGAAAGTGCC 
AGTATGTCTA GCACATGTGA ACCTTGCAAA AGTAGGAACA GACATTCAGC CCAGACTGAA 
GAGCCTGTTC AAGCAAAAGT ATTCAGCAGA AAGAATCATG AGCAACTGGA AAAAATAATA 
AAATGTAATA GGTCTACAGA AATATCTTCA GAAACTGGGA GTGATTTTTC CATGTTTGAA 
GCTTTGCGAG ATACTATTTA TTCTGAAGTA GCTACATTAA TTTCTCAAAA TGAATCTCGT 
CCACATTTTC TTATTGAACT CTTCCATGAG CTGCAGCTAC TAAACACAGA CTACTTGAGA 
CAGAGGGCTT TATATGCATT GCAGGACATA GTATCCAGAC ATATTTCTGA GAGCCATGAA 
AAAGGAGAAA ATGTAAAGTC AGTAAACTCT GGTACTTGGA TAGCATCAAA CTCAGAACTT 
ACTCCTAGTG AGAGCCTTGC TACTACTGAT GATGAAACTT TTGAGAAGAA CTTTGAAAGA 
GAAACCCATA AAATAAGTGA GCAAAATGAT GCTGATAATG CTAGTGTCCT GTCTGTATCA 
TCAAATTTTG AGCCTTTTGC AACAGATGAT CTAGGTAACA CCGTGATTCA CTTAGATCAA 
GCATTAGCCA GAATGAGAGA ATATGAGCGT ATGAAGACTG AGGCTGAAAG TAACTCAAAT 
ATGAGATGCA CCTGCAGGAT TATTGAGGAT GGAGATGGTG CTGGTGCAGA AACTCCCGTT 
ATTGAAAATC GTAGTTCACA ACAACCTGTA AGTGAAGTTT CTACCATCCC ATGTCCTAGA 
ATTGATACTC AGCAGCTGGA CCGGCAAATT AAAGCAATTA TGAAAGAAGT CATTCCTTTT 
TTGAAGGAGC ACATGGATGA AGTATGCTCC TCGCAGCTTC TAACTTCAGT AAGGCGCATG 
GTTTTGACCC TTACCCAGCA AAATGATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TCAATGAATT GGCTTTCTTT 
AAGCTTATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCAA CTGGAGTGAT ACAATCTTGT GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT 
CATGGCTCAC CTGCTGGAGA GATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCAGA CTCAAACATC TGAGGTGTAT GATGGTCCCA AAAATGTAAG ATCTGATATT 
TCTGATCAAG AGGAAGATGA AGAAAGTGAA GGATGTCCAG TGTCTATTAA TTTGTCTAAA 
GCTGAAACTC AGGCTTTAAC TAATTATGGA AGTGGAGAAG ATGAAAATGA GGATGAAGAA 
ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT GTCCAGACTT CCCTCCAGGC TAACACTGAA 
GCTACTGAAG AAAATGAACA TGATGAACAG GTCCTACAAC GTGACTTTAA AAAGACAGCA 
GAAAGCAAAA ATGTCCCATT GGAACGAGAA GCCACTAGTA AAAATGACCA AAATAACTGT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAGATGAGC AACCTTTAAA TAGTGCTGCC 
CATAAGGAGT CACCTCCTAC TGTTGATTCA ACTCAACAGC CTAACCCTTT GCCGTTACGT 
TTACCTGAAA TGGAACCCTT AGTGCCTAGA GTCAAAGAAG TTAAATCTGC TCAGGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAATAT AAGTCAAAAG TCTGATGAAG AAGATTTTGT AAAAGTTGAA 
GATTTACCAC TGAAACTGAC AATATATTCA GAGGCAGATC TAAGAAAGAA AATGGTAGAA 
GAAGAACAGA AAAACCATTT ATCTGGTGAA ATATGTGAAA TGCAGACCGA AGAATTAGCT 
GGAAATTCTG AGACACTAAA AGAACCTGAA ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013486.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868975.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X17, mRNA.

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CloneID	OHu98026
Clone ID Related Accession (Same CDS sequence)	XM_017013486.1
Accession Version	XM_017013486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGGT 
ACTACAGTTA ATAATTTAGA AGAAACTCCC GTTATTGAAA ATCGTAGTTC ACAACAACCT 
GTAAGTGAAG TTTCTACCAT CCCATGTCCT AGAATTGATA CTCAGCAGCT GGACCGGCAA 
ATTAAAGCAA TTATGAAAGA AGTCATTCCT TTTTTGAAGG ATTTTTCTCA GGAGCACATG 
GATGAAGTAT GCTCCTCGCA GCTTCTAACT TCAGTAAGGC GCATGGTTTT GACCCTTACC 
CAGCAAAATG ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAACAACT TGGAAGTATA 
TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AAGACTGTGG AGAAGATCTT 
CTTGTAGAGA TATCTGAAGT GTTGTTCAAT GAATTGGCTT TCTTTAAGCT TATGCAAGAT 
TTGGATAATA ATAGTATAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCAACTGGA 
GTGATACAAT CTTGTGCCAA AGAGGCTAAA AGGATTCTTG AAGATCATGG CTCACCTGCT 
GGAGAGATTG ATGATGAAGA CAAAGACAAG GATGAAACTG AAACAGTTAA GCAGACTCAA 
ACATCTGAGG TGTATGATGG TCCCAAAAAT GTAAGATCTG ATATTTCTGA TCAAGAGGAA 
GATGAAGAAA GTGAAGGATG TCCAGTGTCT ATTAATTTGT CTAAAGCTGA AACTCAGGCT 
TTAACTAATT ATGGAAGTGG AGAAGATGAA AATGAGGATG AAGAAATGGA AGAATTTGAA 
GAAGGCCCTG TGGATGTCCA GACTTCCCTC CAGGCTAACA CTGAAGCTAC TGAAGAAAAT 
GAACATGATG AACAGGTCCT ACAACGTGAC TTTAAAAAGA CAGCAGAAAG CAAAAATGTC 
CCATTGGAAC GAGAAGCCAC TAGTAAAAAT GACCAAAATA ACTGTCCTGT GAAACCCTGT 
TACCTCAATA TCTTGGAAGA TGAGCAACCT TTAAATAGTG CTGCCCATAA GGAGTCACCT 
CCTACTGTTG ATTCAACTCA ACAGCCTAAC CCTTTGCCGT TACGTTTACC TGAAATGGAA 
CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA TCTGCTCAGG AAACTCCTGA AAGCTCTCTG 
GCTGGAAGTC CTGATACTGA ATCTCCAGTG TTAGTGAATG ACTATGAAGC AGAATCTGGT 
AATATAAGTC AAAAGTCTGA TGAAGAAGAT TTTGTAAAAG TTGAAGATTT ACCACTGAAA 
CTGACAATAT ATTCAGAGGC AGATCTAAGA AAGAAAATGG TAGAAGAAGA ACAGAAAAAC 
CATTTATCTG GTGAAATATG TGAAATGCAG ACCGAAGAAT TAGCTGGAAA TTCTGAGACA 
CTAAAAGAAC CTGAAACGGT GGGAGCCCAG AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868975.1
CDS	496..6534
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013486.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013486.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGGT 
ACTACAGTTA ATAATTTAGA AGAAACTCCC GTTATTGAAA ATCGTAGTTC ACAACAACCT 
GTAAGTGAAG TTTCTACCAT CCCATGTCCT AGAATTGATA CTCAGCAGCT GGACCGGCAA 
ATTAAAGCAA TTATGAAAGA AGTCATTCCT TTTTTGAAGG ATTTTTCTCA GGAGCACATG 
GATGAAGTAT GCTCCTCGCA GCTTCTAACT TCAGTAAGGC GCATGGTTTT GACCCTTACC 
CAGCAAAATG ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAACAACT TGGAAGTATA 
TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AAGACTGTGG AGAAGATCTT 
CTTGTAGAGA TATCTGAAGT GTTGTTCAAT GAATTGGCTT TCTTTAAGCT TATGCAAGAT 
TTGGATAATA ATAGTATAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCAACTGGA 
GTGATACAAT CTTGTGCCAA AGAGGCTAAA AGGATTCTTG AAGATCATGG CTCACCTGCT 
GGAGAGATTG ATGATGAAGA CAAAGACAAG GATGAAACTG AAACAGTTAA GCAGACTCAA 
ACATCTGAGG TGTATGATGG TCCCAAAAAT GTAAGATCTG ATATTTCTGA TCAAGAGGAA 
GATGAAGAAA GTGAAGGATG TCCAGTGTCT ATTAATTTGT CTAAAGCTGA AACTCAGGCT 
TTAACTAATT ATGGAAGTGG AGAAGATGAA AATGAGGATG AAGAAATGGA AGAATTTGAA 
GAAGGCCCTG TGGATGTCCA GACTTCCCTC CAGGCTAACA CTGAAGCTAC TGAAGAAAAT 
GAACATGATG AACAGGTCCT ACAACGTGAC TTTAAAAAGA CAGCAGAAAG CAAAAATGTC 
CCATTGGAAC GAGAAGCCAC TAGTAAAAAT GACCAAAATA ACTGTCCTGT GAAACCCTGT 
TACCTCAATA TCTTGGAAGA TGAGCAACCT TTAAATAGTG CTGCCCATAA GGAGTCACCT 
CCTACTGTTG ATTCAACTCA ACAGCCTAAC CCTTTGCCGT TACGTTTACC TGAAATGGAA 
CCCTTAGTGC CTAGAGTCAA AGAAGTTAAA TCTGCTCAGG AAACTCCTGA AAGCTCTCTG 
GCTGGAAGTC CTGATACTGA ATCTCCAGTG TTAGTGAATG ACTATGAAGC AGAATCTGGT 
AATATAAGTC AAAAGTCTGA TGAAGAAGAT TTTGTAAAAG TTGAAGATTT ACCACTGAAA 
CTGACAATAT ATTCAGAGGC AGATCTAAGA AAGAAAATGG TAGAAGAAGA ACAGAAAAAC 
CATTTATCTG GTGAAATATG TGAAATGCAG ACCGAAGAAT TAGCTGGAAA TTCTGAGACA 
CTAAAAGAAC CTGAAACGGT GGGAGCCCAG AGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013488.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868977.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X27, mRNA.

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CloneID	OHu98028
Clone ID Related Accession (Same CDS sequence)	XM_017013488.1 , NM_001352637.2 , XM_017013489.1
Accession Version	XM_017013488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pericentriolar material 1 protein isoform X21
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGGT 
ACTACAGTTA ATAATTTAGA AGAAACTCCC GTTATTGAAA ATCGTAGTTC ACAACAACCT 
GTAAGTGAAG TTTCTACCAT CCCATGTCCT AGAATTGATA CTCAGCAGCT GGACCGGCAA 
ATTAAAGCAA TTATGAAAGA AGTCATTCCT TTTTTGAAGG AGCACATGGA TGAAGTATGC 
TCCTCGCAGC TTCTAACTTC AGTAAGGCGC ATGGTTTTGA CCCTTACCCA GCAAAATGAT 
GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT AAACAACTTG GAAGTATATT ACAGGATTCA 
CTGGCAAAAT TTGCTGGCAG AAAACTGAAA GACTGTGGAG AAGATCTTCT TGTAGAGATA 
TCTGAAGTGT TGTTCAATGA ATTGGCTTTC TTTAAGCTTA TGCAAGATTT GGATAATAAT 
AGTATAACTG TTAAACAGAG ATGCAAAAGG AAAATAGAAG CAACTGGAGT GATACAATCT 
TGTGCCAAAG AGGCTAAAAG GATTCTTGAA GATCATGGCT CACCTGCTGG AGAGATTGAT 
GATGAAGACA AAGACAAGGA TGAAACTGAA ACAGTTAAGC AGACTCAAAC ATCTGAGGTG 
TATGATGGTC CCAAAAATGT AAGATCTGAT ATTTCTGATC AAGAGGAAGA TGAAGAAAGT 
GAAGGATGTC CAGTGTCTAT TAATTTGTCT AAAGCTGAAA CTCAGGCTTT AACTAATTAT 
GGAAGTGGAG AAGATGAAAA TGAGGATGAA GAAATGGAAG AATTTGAAGA AGGCCCTGTG 
GATGTCCAGA CTTCCCTCCA GGCTAACACT GAAGCTACTG AAGAAAATGA ACATGATGAA 
CAGGTCCTAC AACGTGACTT TAAAAAGACA GCAGAAAGCA AAAATGTCCC ATTGGAACGA 
GAAGCCACTA GTAAAAATGA CCAAAATAAC TGTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAGATG AGCAACCTTT AAATAGTGCT GCCCATAAGG AGTCACCTCC TACTGTTGAT 
TCAACTCAAC AGCCTAACCC TTTGCCGTTA CGTTTACCTG AAATGGAACC CTTAGTGCCT 
AGAGTCAAAG AAGTTAAATC TGCTCAGGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA TATAAGTCAA 
AAGTCTGATG AAGAAGATTT TGTAAAAGTT GAAGATTTAC CACTGAAACT GACAATATAT 
TCAGAGGCAG ATCTAAGAAA GAAAATGGTA GAAGAAGAAC AGAAAAACCA TTTATCTGGT 
GAAATATGTG AAATGCAGAC CGAAGAATTA GCTGGAAATT CTGAGACACT AAAAGAACCT 
GAAACGGTGG GAGCCCAGAG TATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868977.1
CDS	496..6522
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013488.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013488.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGGT 
ACTACAGTTA ATAATTTAGA AGAAACTCCC GTTATTGAAA ATCGTAGTTC ACAACAACCT 
GTAAGTGAAG TTTCTACCAT CCCATGTCCT AGAATTGATA CTCAGCAGCT GGACCGGCAA 
ATTAAAGCAA TTATGAAAGA AGTCATTCCT TTTTTGAAGG AGCACATGGA TGAAGTATGC 
TCCTCGCAGC TTCTAACTTC AGTAAGGCGC ATGGTTTTGA CCCTTACCCA GCAAAATGAT 
GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT AAACAACTTG GAAGTATATT ACAGGATTCA 
CTGGCAAAAT TTGCTGGCAG AAAACTGAAA GACTGTGGAG AAGATCTTCT TGTAGAGATA 
TCTGAAGTGT TGTTCAATGA ATTGGCTTTC TTTAAGCTTA TGCAAGATTT GGATAATAAT 
AGTATAACTG TTAAACAGAG ATGCAAAAGG AAAATAGAAG CAACTGGAGT GATACAATCT 
TGTGCCAAAG AGGCTAAAAG GATTCTTGAA GATCATGGCT CACCTGCTGG AGAGATTGAT 
GATGAAGACA AAGACAAGGA TGAAACTGAA ACAGTTAAGC AGACTCAAAC ATCTGAGGTG 
TATGATGGTC CCAAAAATGT AAGATCTGAT ATTTCTGATC AAGAGGAAGA TGAAGAAAGT 
GAAGGATGTC CAGTGTCTAT TAATTTGTCT AAAGCTGAAA CTCAGGCTTT AACTAATTAT 
GGAAGTGGAG AAGATGAAAA TGAGGATGAA GAAATGGAAG AATTTGAAGA AGGCCCTGTG 
GATGTCCAGA CTTCCCTCCA GGCTAACACT GAAGCTACTG AAGAAAATGA ACATGATGAA 
CAGGTCCTAC AACGTGACTT TAAAAAGACA GCAGAAAGCA AAAATGTCCC ATTGGAACGA 
GAAGCCACTA GTAAAAATGA CCAAAATAAC TGTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAGATG AGCAACCTTT AAATAGTGCT GCCCATAAGG AGTCACCTCC TACTGTTGAT 
TCAACTCAAC AGCCTAACCC TTTGCCGTTA CGTTTACCTG AAATGGAACC CTTAGTGCCT 
AGAGTCAAAG AAGTTAAATC TGCTCAGGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA TATAAGTCAA 
AAGTCTGATG AAGAAGATTT TGTAAAAGTT GAAGATTTAC CACTGAAACT GACAATATAT 
TCAGAGGCAG ATCTAAGAAA GAAAATGGTA GAAGAAGAAC AGAAAAACCA TTTATCTGGT 
GAAATATGTG AAATGCAGAC CGAAGAATTA GCTGGAAATT CTGAGACACT AAAAGAACCT 
GAAACGGTGG GAGCCCAGAG TATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013489.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868978.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X18, mRNA.

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CloneID	OHu98028
Clone ID Related Accession (Same CDS sequence)	XM_017013488.1 , NM_001352637.2 , XM_017013489.1
Accession Version	XM_017013489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGGT 
ACTACAGTTA ATAATTTAGA AGAAACTCCC GTTATTGAAA ATCGTAGTTC ACAACAACCT 
GTAAGTGAAG TTTCTACCAT CCCATGTCCT AGAATTGATA CTCAGCAGCT GGACCGGCAA 
ATTAAAGCAA TTATGAAAGA AGTCATTCCT TTTTTGAAGG AGCACATGGA TGAAGTATGC 
TCCTCGCAGC TTCTAACTTC AGTAAGGCGC ATGGTTTTGA CCCTTACCCA GCAAAATGAT 
GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT AAACAACTTG GAAGTATATT ACAGGATTCA 
CTGGCAAAAT TTGCTGGCAG AAAACTGAAA GACTGTGGAG AAGATCTTCT TGTAGAGATA 
TCTGAAGTGT TGTTCAATGA ATTGGCTTTC TTTAAGCTTA TGCAAGATTT GGATAATAAT 
AGTATAACTG TTAAACAGAG ATGCAAAAGG AAAATAGAAG CAACTGGAGT GATACAATCT 
TGTGCCAAAG AGGCTAAAAG GATTCTTGAA GATCATGGCT CACCTGCTGG AGAGATTGAT 
GATGAAGACA AAGACAAGGA TGAAACTGAA ACAGTTAAGC AGACTCAAAC ATCTGAGGTG 
TATGATGGTC CCAAAAATGT AAGATCTGAT ATTTCTGATC AAGAGGAAGA TGAAGAAAGT 
GAAGGATGTC CAGTGTCTAT TAATTTGTCT AAAGCTGAAA CTCAGGCTTT AACTAATTAT 
GGAAGTGGAG AAGATGAAAA TGAGGATGAA GAAATGGAAG AATTTGAAGA AGGCCCTGTG 
GATGTCCAGA CTTCCCTCCA GGCTAACACT GAAGCTACTG AAGAAAATGA ACATGATGAA 
CAGGTCCTAC AACGTGACTT TAAAAAGACA GCAGAAAGCA AAAATGTCCC ATTGGAACGA 
GAAGCCACTA GTAAAAATGA CCAAAATAAC TGTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAGATG AGCAACCTTT AAATAGTGCT GCCCATAAGG AGTCACCTCC TACTGTTGAT 
TCAACTCAAC AGCCTAACCC TTTGCCGTTA CGTTTACCTG AAATGGAACC CTTAGTGCCT 
AGAGTCAAAG AAGTTAAATC TGCTCAGGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA TATAAGTCAA 
AAGTCTGATG AAGAAGATTT TGTAAAAGTT GAAGATTTAC CACTGAAACT GACAATATAT 
TCAGAGGCAG ATCTAAGAAA GAAAATGGTA GAAGAAGAAC AGAAAAACCA TTTATCTGGT 
GAAATATGTG AAATGCAGAC CGAAGAATTA GCTGGAAATT CTGAGACACT AAAAGAACCT 
GAAACGGTGG GAGCCCAGAG TATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868978.1
CDS	799..6825
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013489.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013489.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGGT 
ACTACAGTTA ATAATTTAGA AGAAACTCCC GTTATTGAAA ATCGTAGTTC ACAACAACCT 
GTAAGTGAAG TTTCTACCAT CCCATGTCCT AGAATTGATA CTCAGCAGCT GGACCGGCAA 
ATTAAAGCAA TTATGAAAGA AGTCATTCCT TTTTTGAAGG AGCACATGGA TGAAGTATGC 
TCCTCGCAGC TTCTAACTTC AGTAAGGCGC ATGGTTTTGA CCCTTACCCA GCAAAATGAT 
GAGAGCAAAG AGTTTGTAAA GTTCTTTCAT AAACAACTTG GAAGTATATT ACAGGATTCA 
CTGGCAAAAT TTGCTGGCAG AAAACTGAAA GACTGTGGAG AAGATCTTCT TGTAGAGATA 
TCTGAAGTGT TGTTCAATGA ATTGGCTTTC TTTAAGCTTA TGCAAGATTT GGATAATAAT 
AGTATAACTG TTAAACAGAG ATGCAAAAGG AAAATAGAAG CAACTGGAGT GATACAATCT 
TGTGCCAAAG AGGCTAAAAG GATTCTTGAA GATCATGGCT CACCTGCTGG AGAGATTGAT 
GATGAAGACA AAGACAAGGA TGAAACTGAA ACAGTTAAGC AGACTCAAAC ATCTGAGGTG 
TATGATGGTC CCAAAAATGT AAGATCTGAT ATTTCTGATC AAGAGGAAGA TGAAGAAAGT 
GAAGGATGTC CAGTGTCTAT TAATTTGTCT AAAGCTGAAA CTCAGGCTTT AACTAATTAT 
GGAAGTGGAG AAGATGAAAA TGAGGATGAA GAAATGGAAG AATTTGAAGA AGGCCCTGTG 
GATGTCCAGA CTTCCCTCCA GGCTAACACT GAAGCTACTG AAGAAAATGA ACATGATGAA 
CAGGTCCTAC AACGTGACTT TAAAAAGACA GCAGAAAGCA AAAATGTCCC ATTGGAACGA 
GAAGCCACTA GTAAAAATGA CCAAAATAAC TGTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAGATG AGCAACCTTT AAATAGTGCT GCCCATAAGG AGTCACCTCC TACTGTTGAT 
TCAACTCAAC AGCCTAACCC TTTGCCGTTA CGTTTACCTG AAATGGAACC CTTAGTGCCT 
AGAGTCAAAG AAGTTAAATC TGCTCAGGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA TATAAGTCAA 
AAGTCTGATG AAGAAGATTT TGTAAAAGTT GAAGATTTAC CACTGAAACT GACAATATAT 
TCAGAGGCAG ATCTAAGAAA GAAAATGGTA GAAGAAGAAC AGAAAAACCA TTTATCTGGT 
GAAATATGTG AAATGCAGAC CGAAGAATTA GCTGGAAATT CTGAGACACT AAAAGAACCT 
GAAACGGTGG GAGCCCAGAG TATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013490.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868979.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X29, mRNA.

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CloneID	OHu98029
Clone ID Related Accession (Same CDS sequence)	NM_001352638.2 , XM_017013490.1 , XM_017013490.2
Accession Version	XM_017013490.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGAA 
ACTCCCGTTA TTGAAAATCG TAGTTCACAA CAACCTGTAA GTGAAGTTTC TACCATCCCA 
TGTCCTAGAA TTGATACTCA GCAGCTGGAC CGGCAAATTA AAGCAATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGCTCCT CGCAGCTTCT AACTTCAGTA 
AGGCGCATGG TTTTGACCCT TACCCAGCAA AATGATGAGA GCAAAGAGTT TGTAAAGTTC 
TTTCATAAAC AACTTGGAAG TATATTACAG GATTCACTGG CAAAATTTGC TGGCAGAAAA 
CTGAAAGACT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTGTT CAATGAATTG 
GCTTTCTTTA AGCTTATGCA AGATTTGGAT AATAATAGTA TAACTGTTAA ACAGAGATGC 
AAAAGGAAAA TAGAAGCAAC TGGAGTGATA CAATCTTGTG CCAAAGAGGC TAAAAGGATT 
CTTGAAGATC ATGGCTCACC TGCTGGAGAG ATTGATGATG AAGACAAAGA CAAGGATGAA 
ACTGAAACAG TTAAGCAGAC TCAAACATCT GAGGTGTATG ATGGTCCCAA AAATGTAAGA 
TCTGATATTT CTGATCAAGA GGAAGATGAA GAAAGTGAAG GATGTCCAGT GTCTATTAAT 
TTGTCTAAAG CTGAAACTCA GGCTTTAACT AATTATGGAA GTGGAGAAGA TGAAAATGAG 
GATGAAGAAA TGGAAGAATT TGAAGAAGGC CCTGTGGATG TCCAGACTTC CCTCCAGGCT 
AACACTGAAG CTACTGAAGA AAATGAACAT GATGAACAGG TCCTACAACG TGACTTTAAA 
AAGACAGCAG AAAGCAAAAA TGTCCCATTG GAACGAGAAG CCACTAGTAA AAATGACCAA 
AATAACTGTC CTGTGAAACC CTGTTACCTC AATATCTTGG AAGATGAGCA ACCTTTAAAT 
AGTGCTGCCC ATAAGGAGTC ACCTCCTACT GTTGATTCAA CTCAACAGCC TAACCCTTTG 
CCGTTACGTT TACCTGAAAT GGAACCCTTA GTGCCTAGAG TCAAAGAAGT TAAATCTGCT 
CAGGAAACTC CTGAAAGCTC TCTGGCTGGA AGTCCTGATA CTGAATCTCC AGTGTTAGTG 
AATGACTATG AAGCAGAATC TGGTAATATA AGTCAAAAGT CTGATGAAGA AGATTTTGTA 
AAAGTTGAAG ATTTACCACT GAAACTGACA ATATATTCAG AGGCAGATCT AAGAAAGAAA 
ATGGTAGAAG AAGAACAGAA AAACCATTTA TCTGGTGAAA TATGTGAAAT GCAGACCGAA 
GAATTAGCTG GAAATTCTGA GACACTAAAA GAACCTGAAA CGGTGGGAGC CCAGAGTATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868979.1
CDS	799..6801
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013490.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013490.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGAA 
ACTCCCGTTA TTGAAAATCG TAGTTCACAA CAACCTGTAA GTGAAGTTTC TACCATCCCA 
TGTCCTAGAA TTGATACTCA GCAGCTGGAC CGGCAAATTA AAGCAATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGCTCCT CGCAGCTTCT AACTTCAGTA 
AGGCGCATGG TTTTGACCCT TACCCAGCAA AATGATGAGA GCAAAGAGTT TGTAAAGTTC 
TTTCATAAAC AACTTGGAAG TATATTACAG GATTCACTGG CAAAATTTGC TGGCAGAAAA 
CTGAAAGACT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTGTT CAATGAATTG 
GCTTTCTTTA AGCTTATGCA AGATTTGGAT AATAATAGTA TAACTGTTAA ACAGAGATGC 
AAAAGGAAAA TAGAAGCAAC TGGAGTGATA CAATCTTGTG CCAAAGAGGC TAAAAGGATT 
CTTGAAGATC ATGGCTCACC TGCTGGAGAG ATTGATGATG AAGACAAAGA CAAGGATGAA 
ACTGAAACAG TTAAGCAGAC TCAAACATCT GAGGTGTATG ATGGTCCCAA AAATGTAAGA 
TCTGATATTT CTGATCAAGA GGAAGATGAA GAAAGTGAAG GATGTCCAGT GTCTATTAAT 
TTGTCTAAAG CTGAAACTCA GGCTTTAACT AATTATGGAA GTGGAGAAGA TGAAAATGAG 
GATGAAGAAA TGGAAGAATT TGAAGAAGGC CCTGTGGATG TCCAGACTTC CCTCCAGGCT 
AACACTGAAG CTACTGAAGA AAATGAACAT GATGAACAGG TCCTACAACG TGACTTTAAA 
AAGACAGCAG AAAGCAAAAA TGTCCCATTG GAACGAGAAG CCACTAGTAA AAATGACCAA 
AATAACTGTC CTGTGAAACC CTGTTACCTC AATATCTTGG AAGATGAGCA ACCTTTAAAT 
AGTGCTGCCC ATAAGGAGTC ACCTCCTACT GTTGATTCAA CTCAACAGCC TAACCCTTTG 
CCGTTACGTT TACCTGAAAT GGAACCCTTA GTGCCTAGAG TCAAAGAAGT TAAATCTGCT 
CAGGAAACTC CTGAAAGCTC TCTGGCTGGA AGTCCTGATA CTGAATCTCC AGTGTTAGTG 
AATGACTATG AAGCAGAATC TGGTAATATA AGTCAAAAGT CTGATGAAGA AGATTTTGTA 
AAAGTTGAAG ATTTACCACT GAAACTGACA ATATATTCAG AGGCAGATCT AAGAAAGAAA 
ATGGTAGAAG AAGAACAGAA AAACCATTTA TCTGGTGAAA TATGTGAAAT GCAGACCGAA 
GAATTAGCTG GAAATTCTGA GACACTAAAA GAACCTGAAA CGGTGGGAGC CCAGAGTATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013490.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868979.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X20, mRNA.

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CloneID	OHu98029
Clone ID Related Accession (Same CDS sequence)	NM_001352638.2 , XM_017013490.1 , XM_017013490.2
Accession Version	XM_017013490.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017013490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGAA 
ACTCCCGTTA TTGAAAATCG TAGTTCACAA CAACCTGTAA GTGAAGTTTC TACCATCCCA 
TGTCCTAGAA TTGATACTCA GCAGCTGGAC CGGCAAATTA AAGCAATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGCTCCT CGCAGCTTCT AACTTCAGTA 
AGGCGCATGG TTTTGACCCT TACCCAGCAA AATGATGAGA GCAAAGAGTT TGTAAAGTTC 
TTTCATAAAC AACTTGGAAG TATATTACAG GATTCACTGG CAAAATTTGC TGGCAGAAAA 
CTGAAAGACT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTGTT CAATGAATTG 
GCTTTCTTTA AGCTTATGCA AGATTTGGAT AATAATAGTA TAACTGTTAA ACAGAGATGC 
AAAAGGAAAA TAGAAGCAAC TGGAGTGATA CAATCTTGTG CCAAAGAGGC TAAAAGGATT 
CTTGAAGATC ATGGCTCACC TGCTGGAGAG ATTGATGATG AAGACAAAGA CAAGGATGAA 
ACTGAAACAG TTAAGCAGAC TCAAACATCT GAGGTGTATG ATGGTCCCAA AAATGTAAGA 
TCTGATATTT CTGATCAAGA GGAAGATGAA GAAAGTGAAG GATGTCCAGT GTCTATTAAT 
TTGTCTAAAG CTGAAACTCA GGCTTTAACT AATTATGGAA GTGGAGAAGA TGAAAATGAG 
GATGAAGAAA TGGAAGAATT TGAAGAAGGC CCTGTGGATG TCCAGACTTC CCTCCAGGCT 
AACACTGAAG CTACTGAAGA AAATGAACAT GATGAACAGG TCCTACAACG TGACTTTAAA 
AAGACAGCAG AAAGCAAAAA TGTCCCATTG GAACGAGAAG CCACTAGTAA AAATGACCAA 
AATAACTGTC CTGTGAAACC CTGTTACCTC AATATCTTGG AAGATGAGCA ACCTTTAAAT 
AGTGCTGCCC ATAAGGAGTC ACCTCCTACT GTTGATTCAA CTCAACAGCC TAACCCTTTG 
CCGTTACGTT TACCTGAAAT GGAACCCTTA GTGCCTAGAG TCAAAGAAGT TAAATCTGCT 
CAGGAAACTC CTGAAAGCTC TCTGGCTGGA AGTCCTGATA CTGAATCTCC AGTGTTAGTG 
AATGACTATG AAGCAGAATC TGGTAATATA AGTCAAAAGT CTGATGAAGA AGATTTTGTA 
AAAGTTGAAG ATTTACCACT GAAACTGACA ATATATTCAG AGGCAGATCT AAGAAAGAAA 
ATGGTAGAAG AAGAACAGAA AAACCATTTA TCTGGTGAAA TATGTGAAAT GCAGACCGAA 
GAATTAGCTG GAAATTCTGA GACACTAAAA GAACCTGAAA CGGTGGGAGC CCAGAGTATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868979.1
CDS	799..6801
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIKTGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013490.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013490.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGAGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCGTC CACATTTTCT TATTGAACTC TTCCATGAGC TGCAGCTACT AAACACAGAC 
TACTTGAGAC AGAGGGCTTT ATATGCATTG CAGGACATAG TATCCAGACA TATTTCTGAG 
AGCCATGAAA AAGGAGAAAA TGTAAAGTCA GTAAACTCTG GTACTTGGAT AGCATCAAAC 
TCAGAACTTA CTCCTAGTGA GAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC 
TTTGAAAGAG AAACCCATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTCCTG 
TCTGTATCAT CAAATTTTGA GCCTTTTGCA ACAGATGATC TAGGTAACAC CGTGATTCAC 
TTAGATCAAG CATTAGCCAG AATGAGAGAA TATGAGCGTA TGAAGACTGA GGCTGAAAGT 
AACTCAAATA TGAGATGCAC CTGCAGGATT ATTGAGGATG GAGATGGTGC TGGTGCAGAA 
ACTCCCGTTA TTGAAAATCG TAGTTCACAA CAACCTGTAA GTGAAGTTTC TACCATCCCA 
TGTCCTAGAA TTGATACTCA GCAGCTGGAC CGGCAAATTA AAGCAATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGCTCCT CGCAGCTTCT AACTTCAGTA 
AGGCGCATGG TTTTGACCCT TACCCAGCAA AATGATGAGA GCAAAGAGTT TGTAAAGTTC 
TTTCATAAAC AACTTGGAAG TATATTACAG GATTCACTGG CAAAATTTGC TGGCAGAAAA 
CTGAAAGACT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTGTT CAATGAATTG 
GCTTTCTTTA AGCTTATGCA AGATTTGGAT AATAATAGTA TAACTGTTAA ACAGAGATGC 
AAAAGGAAAA TAGAAGCAAC TGGAGTGATA CAATCTTGTG CCAAAGAGGC TAAAAGGATT 
CTTGAAGATC ATGGCTCACC TGCTGGAGAG ATTGATGATG AAGACAAAGA CAAGGATGAA 
ACTGAAACAG TTAAGCAGAC TCAAACATCT GAGGTGTATG ATGGTCCCAA AAATGTAAGA 
TCTGATATTT CTGATCAAGA GGAAGATGAA GAAAGTGAAG GATGTCCAGT GTCTATTAAT 
TTGTCTAAAG CTGAAACTCA GGCTTTAACT AATTATGGAA GTGGAGAAGA TGAAAATGAG 
GATGAAGAAA TGGAAGAATT TGAAGAAGGC CCTGTGGATG TCCAGACTTC CCTCCAGGCT 
AACACTGAAG CTACTGAAGA AAATGAACAT GATGAACAGG TCCTACAACG TGACTTTAAA 
AAGACAGCAG AAAGCAAAAA TGTCCCATTG GAACGAGAAG CCACTAGTAA AAATGACCAA 
AATAACTGTC CTGTGAAACC CTGTTACCTC AATATCTTGG AAGATGAGCA ACCTTTAAAT 
AGTGCTGCCC ATAAGGAGTC ACCTCCTACT GTTGATTCAA CTCAACAGCC TAACCCTTTG 
CCGTTACGTT TACCTGAAAT GGAACCCTTA GTGCCTAGAG TCAAAGAAGT TAAATCTGCT 
CAGGAAACTC CTGAAAGCTC TCTGGCTGGA AGTCCTGATA CTGAATCTCC AGTGTTAGTG 
AATGACTATG AAGCAGAATC TGGTAATATA AGTCAAAAGT CTGATGAAGA AGATTTTGTA 
AAAGTTGAAG ATTTACCACT GAAACTGACA ATATATTCAG AGGCAGATCT AAGAAAGAAA 
ATGGTAGAAG AAGAACAGAA AAACCATTTA TCTGGTGAAA TATGTGAAAT GCAGACCGAA 
GAATTAGCTG GAAATTCTGA GACACTAAAA GAACCTGAAA CGGTGGGAGC CCAGAGTATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_006197.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006188.3 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 1, mRNA.

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CloneID	OHu18014
Clone ID Related Accession (Same CDS sequence)	NM_006197.3 , NM_001352652.1 , NM_001352653.1
Accession Version	NM_006197.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, L27841.1, AC087625.12, BC133052.1 and AW237120.1. This sequence is a reference standard in the RefSeqGene project. On Mar 27, 2007 this sequence version replaced NM_006197.2. Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.199778.1, SRR1803611.211583.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC CTCGCAGCTT 
CTAACTTCAG TAAGGCGCAT GGTTTTGACC CTTACCCAGC AAAATGATGA GAGCAAAGAG 
TTTGTAAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTCAATGAAT TGGCTTTCTT TAAGCTTATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAGAT GCAAAAGGAA AATAGAAGCA ACTGGAGTGA TACAATCTTG TGCCAAAGAG 
GCTAAAAGGA TTCTTGAAGA TCATGGCTCA CCTGCTGGAG AGATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCAG ACTCAAACAT CTGAGGTGTA TGATGGTCCC 
AAAAATGTAA GATCTGATAT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGGATGTCCA 
GTGTCTATTA ATTTGTCTAA AGCTGAAACT CAGGCTTTAA CTAATTATGG AAGTGGAGAA 
GATGAAAATG AGGATGAAGA AATGGAAGAA TTTGAAGAAG GCCCTGTGGA TGTCCAGACT 
TCCCTCCAGG CTAACACTGA AGCTACTGAA GAAAATGAAC ATGATGAACA GGTCCTACAA 
CGTGACTTTA AAAAGACAGC AGAAAGCAAA AATGTCCCAT TGGAACGAGA AGCCACTAGT 
AAAAATGACC AAAATAACTG TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAGATGAG 
CAACCTTTAA ATAGTGCTGC CCATAAGGAG TCACCTCCTA CTGTTGATTC AACTCAACAG 
CCTAACCCTT TGCCGTTACG TTTACCTGAA ATGGAACCCT TAGTGCCTAG AGTCAAAGAA 
GTTAAATCTG CTCAGGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAATA TAAGTCAAAA GTCTGATGAA 
GAAGATTTTG TAAAAGTTGA AGATTTACCA CTGAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGAAAGA AAATGGTAGA AGAAGAACAG AAAAACCATT TATCTGGTGA AATATGTGAA 
ATGCAGACCG AAGAATTAGC TGGAAATTCT GAGACACTAA AAGAACCTGA AACGGTGGGA 
GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006188.3
CDS	423..6497
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_006197.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006197.3

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC CTCGCAGCTT 
CTAACTTCAG TAAGGCGCAT GGTTTTGACC CTTACCCAGC AAAATGATGA GAGCAAAGAG 
TTTGTAAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTCAATGAAT TGGCTTTCTT TAAGCTTATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAGAT GCAAAAGGAA AATAGAAGCA ACTGGAGTGA TACAATCTTG TGCCAAAGAG 
GCTAAAAGGA TTCTTGAAGA TCATGGCTCA CCTGCTGGAG AGATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCAG ACTCAAACAT CTGAGGTGTA TGATGGTCCC 
AAAAATGTAA GATCTGATAT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGGATGTCCA 
GTGTCTATTA ATTTGTCTAA AGCTGAAACT CAGGCTTTAA CTAATTATGG AAGTGGAGAA 
GATGAAAATG AGGATGAAGA AATGGAAGAA TTTGAAGAAG GCCCTGTGGA TGTCCAGACT 
TCCCTCCAGG CTAACACTGA AGCTACTGAA GAAAATGAAC ATGATGAACA GGTCCTACAA 
CGTGACTTTA AAAAGACAGC AGAAAGCAAA AATGTCCCAT TGGAACGAGA AGCCACTAGT 
AAAAATGACC AAAATAACTG TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAGATGAG 
CAACCTTTAA ATAGTGCTGC CCATAAGGAG TCACCTCCTA CTGTTGATTC AACTCAACAG 
CCTAACCCTT TGCCGTTACG TTTACCTGAA ATGGAACCCT TAGTGCCTAG AGTCAAAGAA 
GTTAAATCTG CTCAGGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAATA TAAGTCAAAA GTCTGATGAA 
GAAGATTTTG TAAAAGTTGA AGATTTACCA CTGAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGAAAGA AAATGGTAGA AGAAGAACAG AAAAACCATT TATCTGGTGA AATATGTGAA 
ATGCAGACCG AAGAATTAGC TGGAAATTCT GAGACACTAA AAGAACCTGA AACGGTGGGA 
GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352652.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339581.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 24, mRNA.

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CloneID	OHu18014
Clone ID Related Accession (Same CDS sequence)	NM_006197.3 , NM_001352652.1 , NM_001352653.1
Accession Version	NM_001352652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 20
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013484.1. Transcript Variant: This variant (24), as well as variant 25, encodes isoform 20. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.20706.1, SRR1803616.129267.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC CTCGCAGCTT 
CTAACTTCAG TAAGGCGCAT GGTTTTGACC CTTACCCAGC AAAATGATGA GAGCAAAGAG 
TTTGTAAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTCAATGAAT TGGCTTTCTT TAAGCTTATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAGAT GCAAAAGGAA AATAGAAGCA ACTGGAGTGA TACAATCTTG TGCCAAAGAG 
GCTAAAAGGA TTCTTGAAGA TCATGGCTCA CCTGCTGGAG AGATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCAG ACTCAAACAT CTGAGGTGTA TGATGGTCCC 
AAAAATGTAA GATCTGATAT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGGATGTCCA 
GTGTCTATTA ATTTGTCTAA AGCTGAAACT CAGGCTTTAA CTAATTATGG AAGTGGAGAA 
GATGAAAATG AGGATGAAGA AATGGAAGAA TTTGAAGAAG GCCCTGTGGA TGTCCAGACT 
TCCCTCCAGG CTAACACTGA AGCTACTGAA GAAAATGAAC ATGATGAACA GGTCCTACAA 
CGTGACTTTA AAAAGACAGC AGAAAGCAAA AATGTCCCAT TGGAACGAGA AGCCACTAGT 
AAAAATGACC AAAATAACTG TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAGATGAG 
CAACCTTTAA ATAGTGCTGC CCATAAGGAG TCACCTCCTA CTGTTGATTC AACTCAACAG 
CCTAACCCTT TGCCGTTACG TTTACCTGAA ATGGAACCCT TAGTGCCTAG AGTCAAAGAA 
GTTAAATCTG CTCAGGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAATA TAAGTCAAAA GTCTGATGAA 
GAAGATTTTG TAAAAGTTGA AGATTTACCA CTGAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGAAAGA AAATGGTAGA AGAAGAACAG AAAAACCATT TATCTGGTGA AATATGTGAA 
ATGCAGACCG AAGAATTAGC TGGAAATTCT GAGACACTAA AAGAACCTGA AACGGTGGGA 
GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339581.1
CDS	355..6429
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352652.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352652.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC CTCGCAGCTT 
CTAACTTCAG TAAGGCGCAT GGTTTTGACC CTTACCCAGC AAAATGATGA GAGCAAAGAG 
TTTGTAAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTCAATGAAT TGGCTTTCTT TAAGCTTATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAGAT GCAAAAGGAA AATAGAAGCA ACTGGAGTGA TACAATCTTG TGCCAAAGAG 
GCTAAAAGGA TTCTTGAAGA TCATGGCTCA CCTGCTGGAG AGATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCAG ACTCAAACAT CTGAGGTGTA TGATGGTCCC 
AAAAATGTAA GATCTGATAT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGGATGTCCA 
GTGTCTATTA ATTTGTCTAA AGCTGAAACT CAGGCTTTAA CTAATTATGG AAGTGGAGAA 
GATGAAAATG AGGATGAAGA AATGGAAGAA TTTGAAGAAG GCCCTGTGGA TGTCCAGACT 
TCCCTCCAGG CTAACACTGA AGCTACTGAA GAAAATGAAC ATGATGAACA GGTCCTACAA 
CGTGACTTTA AAAAGACAGC AGAAAGCAAA AATGTCCCAT TGGAACGAGA AGCCACTAGT 
AAAAATGACC AAAATAACTG TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAGATGAG 
CAACCTTTAA ATAGTGCTGC CCATAAGGAG TCACCTCCTA CTGTTGATTC AACTCAACAG 
CCTAACCCTT TGCCGTTACG TTTACCTGAA ATGGAACCCT TAGTGCCTAG AGTCAAAGAA 
GTTAAATCTG CTCAGGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAATA TAAGTCAAAA GTCTGATGAA 
GAAGATTTTG TAAAAGTTGA AGATTTACCA CTGAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGAAAGA AAATGGTAGA AGAAGAACAG AAAAACCATT TATCTGGTGA AATATGTGAA 
ATGCAGACCG AAGAATTAGC TGGAAATTCT GAGACACTAA AAGAACCTGA AACGGTGGGA 
GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( NM_001352653.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339582.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant 25, mRNA.

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CloneID	OHu18014
Clone ID Related Accession (Same CDS sequence)	NM_006197.3 , NM_001352652.1 , NM_001352653.1
Accession Version	NM_001352653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform 20
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087273.5, BC065022.1 and AC087625.12. On Jun 20, 2017 this sequence version replaced XM_017013483.1. Transcript Variant: This variant (25), as well as variant 24, encodes isoform 20. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.218103.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC CTCGCAGCTT 
CTAACTTCAG TAAGGCGCAT GGTTTTGACC CTTACCCAGC AAAATGATGA GAGCAAAGAG 
TTTGTAAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTCAATGAAT TGGCTTTCTT TAAGCTTATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAGAT GCAAAAGGAA AATAGAAGCA ACTGGAGTGA TACAATCTTG TGCCAAAGAG 
GCTAAAAGGA TTCTTGAAGA TCATGGCTCA CCTGCTGGAG AGATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCAG ACTCAAACAT CTGAGGTGTA TGATGGTCCC 
AAAAATGTAA GATCTGATAT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGGATGTCCA 
GTGTCTATTA ATTTGTCTAA AGCTGAAACT CAGGCTTTAA CTAATTATGG AAGTGGAGAA 
GATGAAAATG AGGATGAAGA AATGGAAGAA TTTGAAGAAG GCCCTGTGGA TGTCCAGACT 
TCCCTCCAGG CTAACACTGA AGCTACTGAA GAAAATGAAC ATGATGAACA GGTCCTACAA 
CGTGACTTTA AAAAGACAGC AGAAAGCAAA AATGTCCCAT TGGAACGAGA AGCCACTAGT 
AAAAATGACC AAAATAACTG TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAGATGAG 
CAACCTTTAA ATAGTGCTGC CCATAAGGAG TCACCTCCTA CTGTTGATTC AACTCAACAG 
CCTAACCCTT TGCCGTTACG TTTACCTGAA ATGGAACCCT TAGTGCCTAG AGTCAAAGAA 
GTTAAATCTG CTCAGGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAATA TAAGTCAAAA GTCTGATGAA 
GAAGATTTTG TAAAAGTTGA AGATTTACCA CTGAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGAAAGA AAATGGTAGA AGAAGAACAG AAAAACCATT TATCTGGTGA AATATGTGAA 
ATGCAGACCG AAGAATTAGC TGGAAATTCT GAGACACTAA AAGAACCTGA AACGGTGGGA 
GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339582.1
CDS	760..6834
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	NM_001352653.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant 25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352653.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAGTCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGATTGTC GATATAATAG AGAAGGGGAA CAGGAGATTC ATGTTGCACA AGGTGAAGAT 
GATGAGGAGG AGGAGGAAGA AGCAGAAGAG GAGGGAGTCA GTGGAGCTTC ATTATCTAGT 
CACAGGAGCA GTCTGGTTGA TGAGCATCCA GAAGATGCTG AATTTGAACA GAAGATCAAC 
CGACTTATGG CTGCAAAACA GAAACTTAGA CAGTTACAAG ATCTTGTTGC TATGGTACAG 
GATGATGATG CAGCTCAAGG AGTTATCTCT GCCAGTGCAT CAAATTTGGA TGATTTCTAC 
CCAGCAGAAG AAGACACCAA GCAAAATTCA AATAACACTA GAGGAAATGC CAATAAAACA 
CAGAAAGATA CTGGAGTAAA TGAAAAGGCA AGAGAGAAAT TTTATGAGGC TAAACTACAG 
CAGCAACAGA GAGAGCTAAA ACAATTGCAG GAAGAAAGAA AGAAACTGAT TGACATTCAG 
GAGAAAATTC AAGCATTGCA AACGGCATGC CCTGACTTAC AGCTGTCAGC TGCTAGTGTG 
GGTAACTGTC CCACCAAAAA ATATATGCCA GCTGTTACTT CAACCCCAAC TGTTAATCAA 
CACGAGACCA GTACAAGCAA ATCTGTTTTT GAGCCTGAAG ATTCTTCAAT AGTAGATAAT 
GAGTTGTGGT CAGAAATGAG AAGACATGAA ATGTTGAGGG AGGAGCTGCG ACAGAGAAGA 
AAGCAGCTTG AAGCTCTGAT GGCTGAACAT CAGAGGAGGC AAGGTCTAGC TGAAACTGCA 
TCTCCAGTGG CTGTGTCATT GAGAAGTGAT GGATCTGAGA ACCTATGTAC TCCTCAGCAA 
AGTAGAACAG AAAAAACGAT GGCAACTTGG GGAGGGTCTA CCCAGTGTGC ACTAGATGAA 
GAAGGAGATG AAGACGGTTA CCTTTCTGAA GGAATTGTTC GGACAGATGA AGAGGAGGAA 
GAAGAGCAAG ATGCCAGTTC CAATGATAAC TTTTCTGTGT GTCCTTCTAA CAGTGTGAAT 
CATAACTCCT ACAATGGAAA GGAAACTAAA AATAGGTGGA AGAACAATTG CCCTTTTTCG 
GCAGATGAAA ATTATCGTCC TTTAGCCAAG ACAAGGCAAC AGAATATCAG CATGCAACGG 
CAAGAAAACC TTCGTTGGGT GTCAGAGCTC TCTTACGTAG AAGAGAAAGA ACAATGGCAA 
GAACAAATCA ATCAGCTAAA GAAACAGCTT GATTTTAGTG TCAGTATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACTCT ATCTTGTCTG CTACAAACTC TTCTCACGGG TCCTTACAGT 
GTTATGCCCA GCAATGTTGC ATCTCCTCAA GTACACTTCA TAATGCACCA GTTGAACCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTTCAGA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAGCAAAA TCAGCATCCA GAAAAACCTG GAGGCAAGGA AAGAGGCAGT 
AGTGCATCGC ACCCTCCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC AACACAGCCT 
GTAAATCTCT TCAATATACC TGGATTTACT AACTTTTCAT CATTTGCACC AGGTATGAAT 
TTCAGCCCTT TATTTCCTTC TAATTTTGGA GATTTTTCTC AGAATATCTC TACACCCAGT 
GAACAGCAGC AACCCTTAGC CCAGAATTCT TCAGGAAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC CTCTATTGGA GCAGAGAAAC CAAGGAATAA AAAACTGCCT 
GAAGAGGAGG TGGAAAGCAG TAGGACACCA TGGTTATATG AACAAGAAGG TGAAGTAGAG 
AAACCATTTA TCAAGACTGG ATTTTCAGTG TCTGTAGAAA AATCTACAAG TAGTAACCGC 
AAAAATCAAT TAGATACAAA CGGAAGAAGA CGCCAGTTTG ATGAAGAATC ACTGGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCA ACAACAGTGA CTAAAACATT CAAGACAAGA 
AAAGCGTCTG CACAGGCCAG CCTGGCATCT AAAGATAAAA CTCCCAAGTC AAAAAGTAAG 
AAGAGGAATT CTACTCAGCT GAAAAGCAGA GTTAAAAACA TCAGGTATGA AAGTGCCAGT 
ATGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCATGAGC AACTGGAAAA AATAATAAAA 
TGTAATAGGT CTACAGAAAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGAGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCGTCCA 
CATTTTCTTA TTGAACTCTT CCATGAGCTG CAGCTACTAA ACACAGACTA CTTGAGACAG 
AGGGCTTTAT ATGCATTGCA GGACATAGTA TCCAGACATA TTTCTGAGAG CCATGAAAAA 
GGAGAAAATG TAAAGTCAGT AAACTCTGGT ACTTGGATAG CATCAAACTC AGAACTTACT 
CCTAGTGAGA GCCTTGCTAC TACTGATGAT GAAACTTTTG AGAAGAACTT TGAAAGAGAA 
ACCCATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GTGTCCTGTC TGTATCATCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACCG TGATTCACTT AGATCAAGCA 
TTAGCCAGAA TGAGAGAATA TGAGCGTATG AAGACTGAGG CTGAAAGTAA CTCAAATATG 
AGATGCACCT GCAGGATTAT TGAGGATGGA GATGGTGCTG GTGCAGGTAC TACAGTTAAT 
AATTTAGAAG AAACTCCCGT TATTGAAAAT CGTAGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACCATCC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCAATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC CTCGCAGCTT 
CTAACTTCAG TAAGGCGCAT GGTTTTGACC CTTACCCAGC AAAATGATGA GAGCAAAGAG 
TTTGTAAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTCAATGAAT TGGCTTTCTT TAAGCTTATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAGAT GCAAAAGGAA AATAGAAGCA ACTGGAGTGA TACAATCTTG TGCCAAAGAG 
GCTAAAAGGA TTCTTGAAGA TCATGGCTCA CCTGCTGGAG AGATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCAG ACTCAAACAT CTGAGGTGTA TGATGGTCCC 
AAAAATGTAA GATCTGATAT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGGATGTCCA 
GTGTCTATTA ATTTGTCTAA AGCTGAAACT CAGGCTTTAA CTAATTATGG AAGTGGAGAA 
GATGAAAATG AGGATGAAGA AATGGAAGAA TTTGAAGAAG GCCCTGTGGA TGTCCAGACT 
TCCCTCCAGG CTAACACTGA AGCTACTGAA GAAAATGAAC ATGATGAACA GGTCCTACAA 
CGTGACTTTA AAAAGACAGC AGAAAGCAAA AATGTCCCAT TGGAACGAGA AGCCACTAGT 
AAAAATGACC AAAATAACTG TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAGATGAG 
CAACCTTTAA ATAGTGCTGC CCATAAGGAG TCACCTCCTA CTGTTGATTC AACTCAACAG 
CCTAACCCTT TGCCGTTACG TTTACCTGAA ATGGAACCCT TAGTGCCTAG AGTCAAAGAA 
GTTAAATCTG CTCAGGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAATA TAAGTCAAAA GTCTGATGAA 
GAAGATTTTG TAAAAGTTGA AGATTTACCA CTGAAACTGA CAATATATTC AGAGGCAGAT 
CTAAGAAAGA AAATGGTAGA AGAAGAACAG AAAAACCATT TATCTGGTGA AATATGTGAA 
ATGCAGACCG AAGAATTAGC TGGAAATTCT GAGACACTAA AAGAACCTGA AACGGTGGGA 
GCCCAGAGTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_006716336.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006716399.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X6, mRNA.

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CloneID	OHu42066
Clone ID Related Accession (Same CDS sequence)	XM_006716336.3
Accession Version	XM_006716336.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006716336.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CAGGTATGAA 
AGTGCCAGTA TGTCTAGCAC ATGTGAACCT TGCAAAAGTA GGAACAGACA TTCAGCCCAG 
ACTGAAGAGC CTGTTCAAGC AAAAGTATTC AGCAGAAAGA ATCATGAGCA ACTGGAAAAA 
ATAATAAAAT GTAATAGGTC TACAGAAATA TCTTCAGCAC ATGCTAGGAG AATACTACAG 
CAGTCTAACA GAAATGCATG CAATGAAGCG CCAGAAACTG GGAGTGATTT TTCCATGTTT 
GAAGCTTTGC GAGATACTAT TTATTCTGAA GTAGCTACAT TAATTTCTCA AAATGAATCT 
CGTCCACATT TTCTTATTGA ACTCTTCCAT GAGCTGCAGC TACTAAACAC AGACTACTTG 
AGACAGAGGG CTTTATATGC ATTGCAGGAC ATAGTATCCA GACATATTTC TGAGAGCCAT 
GAAAAAGGAG AAAATGTAAA GTCAGTAAAC TCTGGTACTT GGATAGCATC AAACTCAGAA 
CTTACTCCTA GTGAGAGCCT TGCTACTACT GATGATGAAA CTTTTGAGAA GAACTTTGAA 
AGAGAAACCC ATAAAATAAG TGAGCAAAAT GATGCTGATA ATGCTAGTGT CCTGTCTGTA 
TCATCAAATT TTGAGCCTTT TGCAACAGAT GATCTAGGTA ACACCGTGAT TCACTTAGAT 
CAAGCATTAG CCAGAATGAG AGAATATGAG CGTATGAAGA CTGAGGCTGA AAGTAACTCA 
AATATGAGAT GCACCTGCAG GATTATTGAG GATGGAGATG GTGCTGGTGC AGGTACTACA 
GTTAATAATT TAGAAGAAAC TCCCGTTATT GAAAATCGTA GTTCACAACA ACCTGTAAGT 
GAAGTTTCTA CCATCCCATG TCCTAGAATT GATACTCAGC AGCTGGACCG GCAAATTAAA 
GCAATTATGA AAGAAGTCAT TCCTTTTTTG AAGGATTTTT CTCAGGAGCA CATGGATGAA 
GTATGCTCCT CGCAGCTTCT AACTTCAGTA AGGCGCATGG TTTTGACCCT TACCCAGCAA 
AATGATGAGA GCAAAGAGTT TGTAAAGTTC TTTCATAAAC AACTTGGAAG TATATTACAG 
GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAAGACT GTGGAGAAGA TCTTCTTGTA 
GAGATATCTG AAGTGTTGTT CAATGAATTG GCTTTCTTTA AGCTTATGCA AGATTTGGAT 
AATAATAGTA TAACTGTTAA ACAGAGATGC AAAAGGAAAA TAGAAGCAAC TGGAGTGATA 
CAATCTTGTG CCAAAGAGGC TAAAAGGATT CTTGAAGATC ATGGCTCACC TGCTGGAGAG 
ATTGATGATG AAGACAAAGA CAAGGATGAA ACTGAAACAG TTAAGCAGAC TCAAACATCT 
GAGGTGTATG ATGGTCCCAA AAATGTAAGA TCTGATATTT CTGATCAAGA GGAAGATGAA 
GAAAGTGAAG GATGTCCAGT GTCTATTAAT TTGTCTAAAG CTGAAACTCA GGCTTTAACT 
AATTATGGAA GTGGAGAAGA TGAAAATGAG GATGAAGAAA TGGAAGAATT TGAAGAAGGC 
CCTGTGGATG TCCAGACTTC CCTCCAGGCT AACACTGAAG CTACTGAAGA AAATGAACAT 
GATGAACAGG TCCTACAACG TGACTTTAAA AAGACAGCAG AAAGCAAAAA TGTCCCATTG 
GAACGAGAAG CCACTAGTAA AAATGACCAA AATAACTGTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAGATGAGCA ACCTTTAAAT AGTGCTGCCC ATAAGGAGTC ACCTCCTACT 
GTTGATTCAA CTCAACAGCC TAACCCTTTG CCGTTACGTT TACCTGAAAT GGAACCCTTA 
GTGCCTAGAG TCAAAGAAGT TAAATCTGCT CAGGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAATATA 
AGTCAAAAGT CTGATGAAGA AGATTTTGTA AAAGTTGAAG ATTTACCACT GAAACTGACA 
ATATATTCAG AGGCAGATCT AAGAAAGAAA ATGGTAGAAG AAGAACAGAA AAACCATTTA 
TCTGGTGAAA TATGTGAAAT GCAGACCGAA GAATTAGCTG GAAATTCTGA GACACTAAAA 
GAACCTGAAA CGGTGGGAGC CCAGAGTATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006716399.1
CDS	496..6948
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEEASFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPQNDRELRPNRKYSQKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_006716336.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006716336.3

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CAGGTATGAA 
AGTGCCAGTA TGTCTAGCAC ATGTGAACCT TGCAAAAGTA GGAACAGACA TTCAGCCCAG 
ACTGAAGAGC CTGTTCAAGC AAAAGTATTC AGCAGAAAGA ATCATGAGCA ACTGGAAAAA 
ATAATAAAAT GTAATAGGTC TACAGAAATA TCTTCAGCAC ATGCTAGGAG AATACTACAG 
CAGTCTAACA GAAATGCATG CAATGAAGCG CCAGAAACTG GGAGTGATTT TTCCATGTTT 
GAAGCTTTGC GAGATACTAT TTATTCTGAA GTAGCTACAT TAATTTCTCA AAATGAATCT 
CGTCCACATT TTCTTATTGA ACTCTTCCAT GAGCTGCAGC TACTAAACAC AGACTACTTG 
AGACAGAGGG CTTTATATGC ATTGCAGGAC ATAGTATCCA GACATATTTC TGAGAGCCAT 
GAAAAAGGAG AAAATGTAAA GTCAGTAAAC TCTGGTACTT GGATAGCATC AAACTCAGAA 
CTTACTCCTA GTGAGAGCCT TGCTACTACT GATGATGAAA CTTTTGAGAA GAACTTTGAA 
AGAGAAACCC ATAAAATAAG TGAGCAAAAT GATGCTGATA ATGCTAGTGT CCTGTCTGTA 
TCATCAAATT TTGAGCCTTT TGCAACAGAT GATCTAGGTA ACACCGTGAT TCACTTAGAT 
CAAGCATTAG CCAGAATGAG AGAATATGAG CGTATGAAGA CTGAGGCTGA AAGTAACTCA 
AATATGAGAT GCACCTGCAG GATTATTGAG GATGGAGATG GTGCTGGTGC AGGTACTACA 
GTTAATAATT TAGAAGAAAC TCCCGTTATT GAAAATCGTA GTTCACAACA ACCTGTAAGT 
GAAGTTTCTA CCATCCCATG TCCTAGAATT GATACTCAGC AGCTGGACCG GCAAATTAAA 
GCAATTATGA AAGAAGTCAT TCCTTTTTTG AAGGATTTTT CTCAGGAGCA CATGGATGAA 
GTATGCTCCT CGCAGCTTCT AACTTCAGTA AGGCGCATGG TTTTGACCCT TACCCAGCAA 
AATGATGAGA GCAAAGAGTT TGTAAAGTTC TTTCATAAAC AACTTGGAAG TATATTACAG 
GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAAGACT GTGGAGAAGA TCTTCTTGTA 
GAGATATCTG AAGTGTTGTT CAATGAATTG GCTTTCTTTA AGCTTATGCA AGATTTGGAT 
AATAATAGTA TAACTGTTAA ACAGAGATGC AAAAGGAAAA TAGAAGCAAC TGGAGTGATA 
CAATCTTGTG CCAAAGAGGC TAAAAGGATT CTTGAAGATC ATGGCTCACC TGCTGGAGAG 
ATTGATGATG AAGACAAAGA CAAGGATGAA ACTGAAACAG TTAAGCAGAC TCAAACATCT 
GAGGTGTATG ATGGTCCCAA AAATGTAAGA TCTGATATTT CTGATCAAGA GGAAGATGAA 
GAAAGTGAAG GATGTCCAGT GTCTATTAAT TTGTCTAAAG CTGAAACTCA GGCTTTAACT 
AATTATGGAA GTGGAGAAGA TGAAAATGAG GATGAAGAAA TGGAAGAATT TGAAGAAGGC 
CCTGTGGATG TCCAGACTTC CCTCCAGGCT AACACTGAAG CTACTGAAGA AAATGAACAT 
GATGAACAGG TCCTACAACG TGACTTTAAA AAGACAGCAG AAAGCAAAAA TGTCCCATTG 
GAACGAGAAG CCACTAGTAA AAATGACCAA AATAACTGTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAGATGAGCA ACCTTTAAAT AGTGCTGCCC ATAAGGAGTC ACCTCCTACT 
GTTGATTCAA CTCAACAGCC TAACCCTTTG CCGTTACGTT TACCTGAAAT GGAACCCTTA 
GTGCCTAGAG TCAAAGAAGT TAAATCTGCT CAGGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAATATA 
AGTCAAAAGT CTGATGAAGA AGATTTTGTA AAAGTTGAAG ATTTACCACT GAAACTGACA 
ATATATTCAG AGGCAGATCT AAGAAAGAAA ATGGTAGAAG AAGAACAGAA AAACCATTTA 
TCTGGTGAAA TATGTGAAAT GCAGACCGAA GAATTAGCTG GAAATTCTGA GACACTAAAA 
GAACCTGAAA CGGTGGGAGC CCAGAGTATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_011544530.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542832.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X3, mRNA.

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CloneID	OHu59523
Clone ID Related Accession (Same CDS sequence)	XM_011544530.2 , XM_011544528.2 , XM_011544529.2 , XM_024447167.1
Accession Version	XM_011544530.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CATTGTCACT 
TTTTTTACTC AAGGGTATGA AAGTGCCAGT ATGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCATGAGC AACTGGAAAA AATAATAAAA TGTAATAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGAGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTGCAG 
CTACTAAACA CAGACTACTT GAGACAGAGG GCTTTATATG CATTGCAGGA CATAGTATCC 
AGACATATTT CTGAGAGCCA TGAAAAAGGA GAAAATGTAA AGTCAGTAAA CTCTGGTACT 
TGGATAGCAT CAAACTCAGA ACTTACTCCT AGTGAGAGCC TTGCTACTAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA AAGAGAAACC CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGTG TCCTGTCTGT ATCATCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACCGTGA TTCACTTAGA TCAAGCATTA GCCAGAATGA GAGAATATGA GCGTATGAAG 
ACTGAGGCTG AAAGTAACTC AAATATGAGA TGCACCTGCA GGATTATTGA GGATGGAGAT 
GGTGCTGGTG CAGGTACTAC AGTTAATAAT TTAGAAGAAA CTCCCGTTAT TGAAAATCGT 
AGTTCACAAC AACCTGTAAG TGAAGTTTCT ACCATCCCAT GTCCTAGAAT TGATACTCAG 
CAGCTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGATTTT 
TCTCAGGAGC ACATGGATGA AGTATGCTCC TCGCAGCTTC TAACTTCAGT AAGGCGCATG 
GTTTTGACCC TTACCCAGCA AAATGATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TCAATGAATT GGCTTTCTTT 
AAGCTTATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCAA CTGGAGTGAT ACAATCTTGT GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT 
CATGGCTCAC CTGCTGGAGA GATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCAGA CTCAAACATC TGAGGTGTAT GATGGTCCCA AAAATGTAAG ATCTGATATT 
TCTGATCAAG AGGAAGATGA AGAAAGTGAA GGATGTCCAG TGTCTATTAA TTTGTCTAAA 
GCTGAAACTC AGGCTTTAAC TAATTATGGA AGTGGAGAAG ATGAAAATGA GGATGAAGAA 
ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT GTCCAGACTT CCCTCCAGGC TAACACTGAA 
GCTACTGAAG AAAATGAACA TGATGAACAG GTCCTACAAC GTGACTTTAA AAAGACAGCA 
GAAAGCAAAA ATGTCCCATT GGAACGAGAA GCCACTAGTA AAAATGACCA AAATAACTGT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAGATGAGC AACCTTTAAA TAGTGCTGCC 
CATAAGGAGT CACCTCCTAC TGTTGATTCA ACTCAACAGC CTAACCCTTT GCCGTTACGT 
TTACCTGAAA TGGAACCCTT AGTGCCTAGA GTCAAAGAAG TTAAATCTGC TCAGGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAATAT AAGTCAAAAG TCTGATGAAG AAGATTTTGT AAAAGTTGAA 
GATTTACCAC TGAAACTGAC AATATATTCA GAGGCAGATC TAAGAAAGAA AATGGTAGAA 
GAAGAACAGA AAAACCATTT ATCTGGTGAA ATATGTGAAA TGCAGACCGA AGAATTAGCT 
GGAAATTCTG AGACACTAAA AGAACCTGAA ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542832.1
CDS	618..7091
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEEASFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPQNDRELRPNRKYSQKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIIVTFFTQGYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_011544530.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544530.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CATTGTCACT 
TTTTTTACTC AAGGGTATGA AAGTGCCAGT ATGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCATGAGC AACTGGAAAA AATAATAAAA TGTAATAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGAGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTGCAG 
CTACTAAACA CAGACTACTT GAGACAGAGG GCTTTATATG CATTGCAGGA CATAGTATCC 
AGACATATTT CTGAGAGCCA TGAAAAAGGA GAAAATGTAA AGTCAGTAAA CTCTGGTACT 
TGGATAGCAT CAAACTCAGA ACTTACTCCT AGTGAGAGCC TTGCTACTAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA AAGAGAAACC CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGTG TCCTGTCTGT ATCATCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACCGTGA TTCACTTAGA TCAAGCATTA GCCAGAATGA GAGAATATGA GCGTATGAAG 
ACTGAGGCTG AAAGTAACTC AAATATGAGA TGCACCTGCA GGATTATTGA GGATGGAGAT 
GGTGCTGGTG CAGGTACTAC AGTTAATAAT TTAGAAGAAA CTCCCGTTAT TGAAAATCGT 
AGTTCACAAC AACCTGTAAG TGAAGTTTCT ACCATCCCAT GTCCTAGAAT TGATACTCAG 
CAGCTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGATTTT 
TCTCAGGAGC ACATGGATGA AGTATGCTCC TCGCAGCTTC TAACTTCAGT AAGGCGCATG 
GTTTTGACCC TTACCCAGCA AAATGATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TCAATGAATT GGCTTTCTTT 
AAGCTTATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCAA CTGGAGTGAT ACAATCTTGT GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT 
CATGGCTCAC CTGCTGGAGA GATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCAGA CTCAAACATC TGAGGTGTAT GATGGTCCCA AAAATGTAAG ATCTGATATT 
TCTGATCAAG AGGAAGATGA AGAAAGTGAA GGATGTCCAG TGTCTATTAA TTTGTCTAAA 
GCTGAAACTC AGGCTTTAAC TAATTATGGA AGTGGAGAAG ATGAAAATGA GGATGAAGAA 
ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT GTCCAGACTT CCCTCCAGGC TAACACTGAA 
GCTACTGAAG AAAATGAACA TGATGAACAG GTCCTACAAC GTGACTTTAA AAAGACAGCA 
GAAAGCAAAA ATGTCCCATT GGAACGAGAA GCCACTAGTA AAAATGACCA AAATAACTGT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAGATGAGC AACCTTTAAA TAGTGCTGCC 
CATAAGGAGT CACCTCCTAC TGTTGATTCA ACTCAACAGC CTAACCCTTT GCCGTTACGT 
TTACCTGAAA TGGAACCCTT AGTGCCTAGA GTCAAAGAAG TTAAATCTGC TCAGGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAATAT AAGTCAAAAG TCTGATGAAG AAGATTTTGT AAAAGTTGAA 
GATTTACCAC TGAAACTGAC AATATATTCA GAGGCAGATC TAAGAAAGAA AATGGTAGAA 
GAAGAACAGA AAAACCATTT ATCTGGTGAA ATATGTGAAA TGCAGACCGA AGAATTAGCT 
GGAAATTCTG AGACACTAAA AGAACCTGAA ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_011544528.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542830.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

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CloneID	OHu59523
Clone ID Related Accession (Same CDS sequence)	XM_011544530.2 , XM_011544528.2 , XM_011544529.2 , XM_024447167.1
Accession Version	XM_011544528.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CATTGTCACT 
TTTTTTACTC AAGGGTATGA AAGTGCCAGT ATGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCATGAGC AACTGGAAAA AATAATAAAA TGTAATAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGAGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTGCAG 
CTACTAAACA CAGACTACTT GAGACAGAGG GCTTTATATG CATTGCAGGA CATAGTATCC 
AGACATATTT CTGAGAGCCA TGAAAAAGGA GAAAATGTAA AGTCAGTAAA CTCTGGTACT 
TGGATAGCAT CAAACTCAGA ACTTACTCCT AGTGAGAGCC TTGCTACTAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA AAGAGAAACC CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGTG TCCTGTCTGT ATCATCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACCGTGA TTCACTTAGA TCAAGCATTA GCCAGAATGA GAGAATATGA GCGTATGAAG 
ACTGAGGCTG AAAGTAACTC AAATATGAGA TGCACCTGCA GGATTATTGA GGATGGAGAT 
GGTGCTGGTG CAGGTACTAC AGTTAATAAT TTAGAAGAAA CTCCCGTTAT TGAAAATCGT 
AGTTCACAAC AACCTGTAAG TGAAGTTTCT ACCATCCCAT GTCCTAGAAT TGATACTCAG 
CAGCTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGATTTT 
TCTCAGGAGC ACATGGATGA AGTATGCTCC TCGCAGCTTC TAACTTCAGT AAGGCGCATG 
GTTTTGACCC TTACCCAGCA AAATGATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TCAATGAATT GGCTTTCTTT 
AAGCTTATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCAA CTGGAGTGAT ACAATCTTGT GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT 
CATGGCTCAC CTGCTGGAGA GATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCAGA CTCAAACATC TGAGGTGTAT GATGGTCCCA AAAATGTAAG ATCTGATATT 
TCTGATCAAG AGGAAGATGA AGAAAGTGAA GGATGTCCAG TGTCTATTAA TTTGTCTAAA 
GCTGAAACTC AGGCTTTAAC TAATTATGGA AGTGGAGAAG ATGAAAATGA GGATGAAGAA 
ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT GTCCAGACTT CCCTCCAGGC TAACACTGAA 
GCTACTGAAG AAAATGAACA TGATGAACAG GTCCTACAAC GTGACTTTAA AAAGACAGCA 
GAAAGCAAAA ATGTCCCATT GGAACGAGAA GCCACTAGTA AAAATGACCA AAATAACTGT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAGATGAGC AACCTTTAAA TAGTGCTGCC 
CATAAGGAGT CACCTCCTAC TGTTGATTCA ACTCAACAGC CTAACCCTTT GCCGTTACGT 
TTACCTGAAA TGGAACCCTT AGTGCCTAGA GTCAAAGAAG TTAAATCTGC TCAGGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAATAT AAGTCAAAAG TCTGATGAAG AAGATTTTGT AAAAGTTGAA 
GATTTACCAC TGAAACTGAC AATATATTCA GAGGCAGATC TAAGAAAGAA AATGGTAGAA 
GAAGAACAGA AAAACCATTT ATCTGGTGAA ATATGTGAAA TGCAGACCGA AGAATTAGCT 
GGAAATTCTG AGACACTAAA AGAACCTGAA ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542830.1
CDS	496..6969
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEEASFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPQNDRELRPNRKYSQKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIIVTFFTQGYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_011544528.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544528.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CATTGTCACT 
TTTTTTACTC AAGGGTATGA AAGTGCCAGT ATGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCATGAGC AACTGGAAAA AATAATAAAA TGTAATAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGAGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTGCAG 
CTACTAAACA CAGACTACTT GAGACAGAGG GCTTTATATG CATTGCAGGA CATAGTATCC 
AGACATATTT CTGAGAGCCA TGAAAAAGGA GAAAATGTAA AGTCAGTAAA CTCTGGTACT 
TGGATAGCAT CAAACTCAGA ACTTACTCCT AGTGAGAGCC TTGCTACTAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA AAGAGAAACC CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGTG TCCTGTCTGT ATCATCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACCGTGA TTCACTTAGA TCAAGCATTA GCCAGAATGA GAGAATATGA GCGTATGAAG 
ACTGAGGCTG AAAGTAACTC AAATATGAGA TGCACCTGCA GGATTATTGA GGATGGAGAT 
GGTGCTGGTG CAGGTACTAC AGTTAATAAT TTAGAAGAAA CTCCCGTTAT TGAAAATCGT 
AGTTCACAAC AACCTGTAAG TGAAGTTTCT ACCATCCCAT GTCCTAGAAT TGATACTCAG 
CAGCTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGATTTT 
TCTCAGGAGC ACATGGATGA AGTATGCTCC TCGCAGCTTC TAACTTCAGT AAGGCGCATG 
GTTTTGACCC TTACCCAGCA AAATGATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TCAATGAATT GGCTTTCTTT 
AAGCTTATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCAA CTGGAGTGAT ACAATCTTGT GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT 
CATGGCTCAC CTGCTGGAGA GATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCAGA CTCAAACATC TGAGGTGTAT GATGGTCCCA AAAATGTAAG ATCTGATATT 
TCTGATCAAG AGGAAGATGA AGAAAGTGAA GGATGTCCAG TGTCTATTAA TTTGTCTAAA 
GCTGAAACTC AGGCTTTAAC TAATTATGGA AGTGGAGAAG ATGAAAATGA GGATGAAGAA 
ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT GTCCAGACTT CCCTCCAGGC TAACACTGAA 
GCTACTGAAG AAAATGAACA TGATGAACAG GTCCTACAAC GTGACTTTAA AAAGACAGCA 
GAAAGCAAAA ATGTCCCATT GGAACGAGAA GCCACTAGTA AAAATGACCA AAATAACTGT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAGATGAGC AACCTTTAAA TAGTGCTGCC 
CATAAGGAGT CACCTCCTAC TGTTGATTCA ACTCAACAGC CTAACCCTTT GCCGTTACGT 
TTACCTGAAA TGGAACCCTT AGTGCCTAGA GTCAAAGAAG TTAAATCTGC TCAGGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAATAT AAGTCAAAAG TCTGATGAAG AAGATTTTGT AAAAGTTGAA 
GATTTACCAC TGAAACTGAC AATATATTCA GAGGCAGATC TAAGAAAGAA AATGGTAGAA 
GAAGAACAGA AAAACCATTT ATCTGGTGAA ATATGTGAAA TGCAGACCGA AGAATTAGCT 
GGAAATTCTG AGACACTAAA AGAACCTGAA ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_011544529.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542831.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

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CloneID	OHu59523
Clone ID Related Accession (Same CDS sequence)	XM_011544530.2 , XM_011544528.2 , XM_011544529.2 , XM_024447167.1
Accession Version	XM_011544529.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544529.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CATTGTCACT 
TTTTTTACTC AAGGGTATGA AAGTGCCAGT ATGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCATGAGC AACTGGAAAA AATAATAAAA TGTAATAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGAGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTGCAG 
CTACTAAACA CAGACTACTT GAGACAGAGG GCTTTATATG CATTGCAGGA CATAGTATCC 
AGACATATTT CTGAGAGCCA TGAAAAAGGA GAAAATGTAA AGTCAGTAAA CTCTGGTACT 
TGGATAGCAT CAAACTCAGA ACTTACTCCT AGTGAGAGCC TTGCTACTAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA AAGAGAAACC CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGTG TCCTGTCTGT ATCATCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACCGTGA TTCACTTAGA TCAAGCATTA GCCAGAATGA GAGAATATGA GCGTATGAAG 
ACTGAGGCTG AAAGTAACTC AAATATGAGA TGCACCTGCA GGATTATTGA GGATGGAGAT 
GGTGCTGGTG CAGGTACTAC AGTTAATAAT TTAGAAGAAA CTCCCGTTAT TGAAAATCGT 
AGTTCACAAC AACCTGTAAG TGAAGTTTCT ACCATCCCAT GTCCTAGAAT TGATACTCAG 
CAGCTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGATTTT 
TCTCAGGAGC ACATGGATGA AGTATGCTCC TCGCAGCTTC TAACTTCAGT AAGGCGCATG 
GTTTTGACCC TTACCCAGCA AAATGATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TCAATGAATT GGCTTTCTTT 
AAGCTTATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCAA CTGGAGTGAT ACAATCTTGT GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT 
CATGGCTCAC CTGCTGGAGA GATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCAGA CTCAAACATC TGAGGTGTAT GATGGTCCCA AAAATGTAAG ATCTGATATT 
TCTGATCAAG AGGAAGATGA AGAAAGTGAA GGATGTCCAG TGTCTATTAA TTTGTCTAAA 
GCTGAAACTC AGGCTTTAAC TAATTATGGA AGTGGAGAAG ATGAAAATGA GGATGAAGAA 
ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT GTCCAGACTT CCCTCCAGGC TAACACTGAA 
GCTACTGAAG AAAATGAACA TGATGAACAG GTCCTACAAC GTGACTTTAA AAAGACAGCA 
GAAAGCAAAA ATGTCCCATT GGAACGAGAA GCCACTAGTA AAAATGACCA AAATAACTGT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAGATGAGC AACCTTTAAA TAGTGCTGCC 
CATAAGGAGT CACCTCCTAC TGTTGATTCA ACTCAACAGC CTAACCCTTT GCCGTTACGT 
TTACCTGAAA TGGAACCCTT AGTGCCTAGA GTCAAAGAAG TTAAATCTGC TCAGGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAATAT AAGTCAAAAG TCTGATGAAG AAGATTTTGT AAAAGTTGAA 
GATTTACCAC TGAAACTGAC AATATATTCA GAGGCAGATC TAAGAAAGAA AATGGTAGAA 
GAAGAACAGA AAAACCATTT ATCTGGTGAA ATATGTGAAA TGCAGACCGA AGAATTAGCT 
GGAAATTCTG AGACACTAAA AGAACCTGAA ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542831.1
CDS	802..7275
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEEASFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPQNDRELRPNRKYSQKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIIVTFFTQGYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_011544529.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544529.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CATTGTCACT 
TTTTTTACTC AAGGGTATGA AAGTGCCAGT ATGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCATGAGC AACTGGAAAA AATAATAAAA TGTAATAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGAGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTGCAG 
CTACTAAACA CAGACTACTT GAGACAGAGG GCTTTATATG CATTGCAGGA CATAGTATCC 
AGACATATTT CTGAGAGCCA TGAAAAAGGA GAAAATGTAA AGTCAGTAAA CTCTGGTACT 
TGGATAGCAT CAAACTCAGA ACTTACTCCT AGTGAGAGCC TTGCTACTAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA AAGAGAAACC CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGTG TCCTGTCTGT ATCATCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACCGTGA TTCACTTAGA TCAAGCATTA GCCAGAATGA GAGAATATGA GCGTATGAAG 
ACTGAGGCTG AAAGTAACTC AAATATGAGA TGCACCTGCA GGATTATTGA GGATGGAGAT 
GGTGCTGGTG CAGGTACTAC AGTTAATAAT TTAGAAGAAA CTCCCGTTAT TGAAAATCGT 
AGTTCACAAC AACCTGTAAG TGAAGTTTCT ACCATCCCAT GTCCTAGAAT TGATACTCAG 
CAGCTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGATTTT 
TCTCAGGAGC ACATGGATGA AGTATGCTCC TCGCAGCTTC TAACTTCAGT AAGGCGCATG 
GTTTTGACCC TTACCCAGCA AAATGATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TCAATGAATT GGCTTTCTTT 
AAGCTTATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCAA CTGGAGTGAT ACAATCTTGT GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT 
CATGGCTCAC CTGCTGGAGA GATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCAGA CTCAAACATC TGAGGTGTAT GATGGTCCCA AAAATGTAAG ATCTGATATT 
TCTGATCAAG AGGAAGATGA AGAAAGTGAA GGATGTCCAG TGTCTATTAA TTTGTCTAAA 
GCTGAAACTC AGGCTTTAAC TAATTATGGA AGTGGAGAAG ATGAAAATGA GGATGAAGAA 
ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT GTCCAGACTT CCCTCCAGGC TAACACTGAA 
GCTACTGAAG AAAATGAACA TGATGAACAG GTCCTACAAC GTGACTTTAA AAAGACAGCA 
GAAAGCAAAA ATGTCCCATT GGAACGAGAA GCCACTAGTA AAAATGACCA AAATAACTGT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAGATGAGC AACCTTTAAA TAGTGCTGCC 
CATAAGGAGT CACCTCCTAC TGTTGATTCA ACTCAACAGC CTAACCCTTT GCCGTTACGT 
TTACCTGAAA TGGAACCCTT AGTGCCTAGA GTCAAAGAAG TTAAATCTGC TCAGGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAATAT AAGTCAAAAG TCTGATGAAG AAGATTTTGT AAAAGTTGAA 
GATTTACCAC TGAAACTGAC AATATATTCA GAGGCAGATC TAAGAAAGAA AATGGTAGAA 
GAAGAACAGA AAAACCATTT ATCTGGTGAA ATATGTGAAA TGCAGACCGA AGAATTAGCT 
GGAAATTCTG AGACACTAAA AGAACCTGAA ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_024447167.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302935.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X4, mRNA.

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CloneID	OHu59523
Clone ID Related Accession (Same CDS sequence)	XM_011544530.2 , XM_011544528.2 , XM_011544529.2 , XM_024447167.1
Accession Version	XM_024447167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CATTGTCACT 
TTTTTTACTC AAGGGTATGA AAGTGCCAGT ATGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCATGAGC AACTGGAAAA AATAATAAAA TGTAATAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGAGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTGCAG 
CTACTAAACA CAGACTACTT GAGACAGAGG GCTTTATATG CATTGCAGGA CATAGTATCC 
AGACATATTT CTGAGAGCCA TGAAAAAGGA GAAAATGTAA AGTCAGTAAA CTCTGGTACT 
TGGATAGCAT CAAACTCAGA ACTTACTCCT AGTGAGAGCC TTGCTACTAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA AAGAGAAACC CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGTG TCCTGTCTGT ATCATCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACCGTGA TTCACTTAGA TCAAGCATTA GCCAGAATGA GAGAATATGA GCGTATGAAG 
ACTGAGGCTG AAAGTAACTC AAATATGAGA TGCACCTGCA GGATTATTGA GGATGGAGAT 
GGTGCTGGTG CAGGTACTAC AGTTAATAAT TTAGAAGAAA CTCCCGTTAT TGAAAATCGT 
AGTTCACAAC AACCTGTAAG TGAAGTTTCT ACCATCCCAT GTCCTAGAAT TGATACTCAG 
CAGCTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGATTTT 
TCTCAGGAGC ACATGGATGA AGTATGCTCC TCGCAGCTTC TAACTTCAGT AAGGCGCATG 
GTTTTGACCC TTACCCAGCA AAATGATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TCAATGAATT GGCTTTCTTT 
AAGCTTATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCAA CTGGAGTGAT ACAATCTTGT GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT 
CATGGCTCAC CTGCTGGAGA GATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCAGA CTCAAACATC TGAGGTGTAT GATGGTCCCA AAAATGTAAG ATCTGATATT 
TCTGATCAAG AGGAAGATGA AGAAAGTGAA GGATGTCCAG TGTCTATTAA TTTGTCTAAA 
GCTGAAACTC AGGCTTTAAC TAATTATGGA AGTGGAGAAG ATGAAAATGA GGATGAAGAA 
ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT GTCCAGACTT CCCTCCAGGC TAACACTGAA 
GCTACTGAAG AAAATGAACA TGATGAACAG GTCCTACAAC GTGACTTTAA AAAGACAGCA 
GAAAGCAAAA ATGTCCCATT GGAACGAGAA GCCACTAGTA AAAATGACCA AAATAACTGT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAGATGAGC AACCTTTAAA TAGTGCTGCC 
CATAAGGAGT CACCTCCTAC TGTTGATTCA ACTCAACAGC CTAACCCTTT GCCGTTACGT 
TTACCTGAAA TGGAACCCTT AGTGCCTAGA GTCAAAGAAG TTAAATCTGC TCAGGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAATAT AAGTCAAAAG TCTGATGAAG AAGATTTTGT AAAAGTTGAA 
GATTTACCAC TGAAACTGAC AATATATTCA GAGGCAGATC TAAGAAAGAA AATGGTAGAA 
GAAGAACAGA AAAACCATTT ATCTGGTGAA ATATGTGAAA TGCAGACCGA AGAATTAGCT 
GGAAATTCTG AGACACTAAA AGAACCTGAA ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302935.1
CDS	1348..7821
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEEASFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPQNDRELRPNRKYSQKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIIVTFFTQGYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_024447167.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447167.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CATTGTCACT 
TTTTTTACTC AAGGGTATGA AAGTGCCAGT ATGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCATGAGC AACTGGAAAA AATAATAAAA TGTAATAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGAGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTGCAG 
CTACTAAACA CAGACTACTT GAGACAGAGG GCTTTATATG CATTGCAGGA CATAGTATCC 
AGACATATTT CTGAGAGCCA TGAAAAAGGA GAAAATGTAA AGTCAGTAAA CTCTGGTACT 
TGGATAGCAT CAAACTCAGA ACTTACTCCT AGTGAGAGCC TTGCTACTAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA AAGAGAAACC CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGTG TCCTGTCTGT ATCATCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACCGTGA TTCACTTAGA TCAAGCATTA GCCAGAATGA GAGAATATGA GCGTATGAAG 
ACTGAGGCTG AAAGTAACTC AAATATGAGA TGCACCTGCA GGATTATTGA GGATGGAGAT 
GGTGCTGGTG CAGGTACTAC AGTTAATAAT TTAGAAGAAA CTCCCGTTAT TGAAAATCGT 
AGTTCACAAC AACCTGTAAG TGAAGTTTCT ACCATCCCAT GTCCTAGAAT TGATACTCAG 
CAGCTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGATTTT 
TCTCAGGAGC ACATGGATGA AGTATGCTCC TCGCAGCTTC TAACTTCAGT AAGGCGCATG 
GTTTTGACCC TTACCCAGCA AAATGATGAG AGCAAAGAGT TTGTAAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TCAATGAATT GGCTTTCTTT 
AAGCTTATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAGATG CAAAAGGAAA 
ATAGAAGCAA CTGGAGTGAT ACAATCTTGT GCCAAAGAGG CTAAAAGGAT TCTTGAAGAT 
CATGGCTCAC CTGCTGGAGA GATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCAGA CTCAAACATC TGAGGTGTAT GATGGTCCCA AAAATGTAAG ATCTGATATT 
TCTGATCAAG AGGAAGATGA AGAAAGTGAA GGATGTCCAG TGTCTATTAA TTTGTCTAAA 
GCTGAAACTC AGGCTTTAAC TAATTATGGA AGTGGAGAAG ATGAAAATGA GGATGAAGAA 
ATGGAAGAAT TTGAAGAAGG CCCTGTGGAT GTCCAGACTT CCCTCCAGGC TAACACTGAA 
GCTACTGAAG AAAATGAACA TGATGAACAG GTCCTACAAC GTGACTTTAA AAAGACAGCA 
GAAAGCAAAA ATGTCCCATT GGAACGAGAA GCCACTAGTA AAAATGACCA AAATAACTGT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAGATGAGC AACCTTTAAA TAGTGCTGCC 
CATAAGGAGT CACCTCCTAC TGTTGATTCA ACTCAACAGC CTAACCCTTT GCCGTTACGT 
TTACCTGAAA TGGAACCCTT AGTGCCTAGA GTCAAAGAAG TTAAATCTGC TCAGGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAATAT AAGTCAAAAG TCTGATGAAG AAGATTTTGT AAAAGTTGAA 
GATTTACCAC TGAAACTGAC AATATATTCA GAGGCAGATC TAAGAAAGAA AATGGTAGAA 
GAAGAACAGA AAAACCATTT ATCTGGTGAA ATATGTGAAA TGCAGACCGA AGAATTAGCT 
GGAAATTCTG AGACACTAAA AGAACCTGAA ACGGTGGGAG CCCAGAGTAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_011544532.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542834.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

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CloneID	OHu59524
Clone ID Related Accession (Same CDS sequence)	XM_011544532.2
Accession Version	XM_011544532.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG ATTGTCGATA TAATAGAGAA GGGGAACAGG AGATTCATGT TGCACAAGGT 
GAAGATGATG AGGAGGAGGA GGAAGAAGCA GAAGAGGAGG GAGTCAGTGG AGCTTCATTA 
TCTAGTCACA GGAGCAGTCT GGTTGATGAG CATCCAGAAG ATGCTGAATT TGAACAGAAG 
ATCAACCGAC TTATGGCTGC AAAACAGAAA CTTAGACAGT TACAAGATCT TGTTGCTATG 
GTACAGGATG ATGATGCAGC TCAAGGAGTT ATCTCTGCCA GTGCATCAAA TTTGGATGAT 
TTCTACCCAG CAGAAGAAGA CACCAAGCAA AATTCAAATA ACACTAGAGG AAATGCCAAT 
AAAACACAGA AAGATACTGG AGTAAATGAA AAGGCAAGAG AGAAATTTTA TGAGGCTAAA 
CTACAGCAGC AACAGAGAGA GCTAAAACAA TTGCAGGAAG AAAGAAAGAA ACTGATTGAC 
ATTCAGGAGA AAATTCAAGC ATTGCAAACG GCATGCCCTG ACTTACAGCT GTCAGCTGCT 
AGTGTGGGTA ACTGTCCCAC CAAAAAATAT ATGCCAGCTG TTACTTCAAC CCCAACTGTT 
AATCAACACG AGACCAGTAC AAGCAAATCT GTTTTTGAGC CTGAAGATTC TTCAATAGTA 
GATAATGAGT TGTGGTCAGA AATGAGAAGA CATGAAATGT TGAGGGAGGA GCTGCGACAG 
AGAAGAAAGC AGCTTGAAGC TCTGATGGCT GAACATCAGA GGAGGCAAGG TCTAGCTGAA 
ACTGCATCTC CAGTGGCTGT GTCATTGAGA AGTGATGGAT CTGAGAACCT ATGTACTCCT 
CAGCAAAGTA GAACAGAAAA AACGATGGCA ACTTGGGGAG GGTCTACCCA GTGTGCACTA 
GATGAAGAAG GAGATGAAGA CGGTTACCTT TCTGAAGGAA TTGTTCGGAC AGATGAAGAG 
GAGGAAGAAG AGCAAGATGC CAGTTCCAAT GATAACTTTT CTGTGTGTCC TTCTAACAGT 
GTGAATCATA ACTCCTACAA TGGAAAGGAA ACTAAAAATA GGTGGAAGAA CAATTGCCCT 
TTTTCGGCAG ATGAAAATTA TCGTCCTTTA GCCAAGACAA GGCAACAGAA TATCAGCATG 
CAACGGCAAG AAAACCTTCG TTGGGTGTCA GAGCTCTCTT ACGTAGAAGA GAAAGAACAA 
TGGCAAGAAC AAATCAATCA GCTAAAGAAA CAGCTTGATT TTAGTGTCAG TATTTGTCAG 
ACTTTGATGC AAGACCAGCA GACTCTATCT TGTCTGCTAC AAACTCTTCT CACGGGTCCT 
TACAGTGTTA TGCCCAGCAA TGTTGCATCT CCTCAAGTAC ACTTCATAAT GCACCAGTTG 
AACCAGTGCT ATACTCAGCT AACATGGCAA CAGAATAATG TTCAGAGGTT GAAACAAATG 
CTAAATGAAC TTATGCGCCA GCAAAATCAG CATCCAGAAA AACCTGGAGG CAAGGAAAGA 
GGCAGTAGTG CATCGCACCC TCCTTCTCCC AGTTTATTTT GTCCTTTCAG CTTTCCAACA 
CAGCCTGTAA ATCTCTTCAA TATACCTGGA TTTACTAACT TTTCATCATT TGCACCAGGT 
ATGAATTTCA GCCCTTTATT TCCTTCTAAT TTTGGAGATT TTTCTCAGAA TATCTCTACA 
CCCAGTGAAC AGCAGCAACC CTTAGCCCAG AATTCTTCAG GAAAAACAGA ATATATGGCT 
TTTCCAAAAC CTTTTGAAAG CAGTTCCTCT ATTGGAGCAG AGAAACCAAG GAATAAAAAA 
CTGCCTGAAG AGGAGGTGGA AAGCAGTAGG ACACCATGGT TATATGAACA AGAAGGTGAA 
GTAGAGAAAC CATTTATCAA GACTGGATTT TCAGTGTCTG TAGAAAAATC TACAAGTAGT 
AACCGCAAAA ATCAATTAGA TACAAACGGA AGAAGACGCC AGTTTGATGA AGAATCACTG 
GAAAGCTTTA GCAGTATGCC TGATCCAGTA GATCCAACAA CAGTGACTAA AACATTCAAG 
ACAAGAAAAG CGTCTGCACA GGCCAGCCTG GCATCTAAAG ATAAAACTCC CAAGTCAAAA 
AGTAAGAAGA GGAATTCTAC TCAGCTGAAA AGCAGAGTTA AAAACATCAT TGTCACTTTT 
TTTACTCAAG GGTATGAAAG TGCCAGTATG TCTAGCACAT GTGAACCTTG CAAAAGTAGG 
AACAGACATT CAGCCCAGAC TGAAGAGCCT GTTCAAGCAA AAGTATTCAG CAGAAAGAAT 
CATGAGCAAC TGGAAAAAAT AATAAAATGT AATAGGTCTA CAGAAATATC TTCAGCACAT 
GCTAGGAGAA TACTACAGCA GTCTAACAGA AATGCATGCA ATGAAGCGCC AGAAACTGGG 
AGTGATTTTT CCATGTTTGA AGCTTTGCGA GATACTATTT ATTCTGAAGT AGCTACATTA 
ATTTCTCAAA ATGAATCTCG TCCACATTTT CTTATTGAAC TCTTCCATGA GCTGCAGCTA 
CTAAACACAG ACTACTTGAG ACAGAGGGCT TTATATGCAT TGCAGGACAT AGTATCCAGA 
CATATTTCTG AGAGCCATGA AAAAGGAGAA AATGTAAAGT CAGTAAACTC TGGTACTTGG 
ATAGCATCAA ACTCAGAACT TACTCCTAGT GAGAGCCTTG CTACTACTGA TGATGAAACT 
TTTGAGAAGA ACTTTGAAAG AGAAACCCAT AAAATAAGTG AGCAAAATGA TGCTGATAAT 
GCTAGTGTCC TGTCTGTATC ATCAAATTTT GAGCCTTTTG CAACAGATGA TCTAGGTAAC 
ACCGTGATTC ACTTAGATCA AGCATTAGCC AGAATGAGAG AATATGAGCG TATGAAGACT 
GAGGCTGAAA GTAACTCAAA TATGAGATGC ACCTGCAGGA TTATTGAGGA TGGAGATGGT 
GCTGGTGCAG GTACTACAGT TAATAATTTA GAAGAAACTC CCGTTATTGA AAATCGTAGT 
TCACAACAAC CTGTAAGTGA AGTTTCTACC ATCCCATGTC CTAGAATTGA TACTCAGCAG 
CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGATTTTTCT 
CAGGAGCACA TGGATGAAGT ATGCTCCTCG CAGCTTCTAA CTTCAGTAAG GCGCATGGTT 
TTGACCCTTA CCCAGCAAAA TGATGAGAGC AAAGAGTTTG TAAAGTTCTT TCATAAACAA 
CTTGGAAGTA TATTACAGGA TTCACTGGCA AAATTTGCTG GCAGAAAACT GAAAGACTGT 
GGAGAAGATC TTCTTGTAGA GATATCTGAA GTGTTGTTCA ATGAATTGGC TTTCTTTAAG 
CTTATGCAAG ATTTGGATAA TAATAGTATA ACTGTTAAAC AGAGATGCAA AAGGAAAATA 
GAAGCAACTG GAGTGATACA ATCTTGTGCC AAAGAGGCTA AAAGGATTCT TGAAGATCAT 
GGCTCACCTG CTGGAGAGAT TGATGATGAA GACAAAGACA AGGATGAAAC TGAAACAGTT 
AAGCAGACTC AAACATCTGA GGTGTATGAT GGTCCCAAAA ATGTAAGATC TGATATTTCT 
GATCAAGAGG AAGATGAAGA AAGTGAAGGA TGTCCAGTGT CTATTAATTT GTCTAAAGCT 
GAAACTCAGG CTTTAACTAA TTATGGAAGT GGAGAAGATG AAAATGAGGA TGAAGAAATG 
GAAGAATTTG AAGAAGGCCC TGTGGATGTC CAGACTTCCC TCCAGGCTAA CACTGAAGCT 
ACTGAAGAAA ATGAACATGA TGAACAGGTC CTACAACGTG ACTTTAAAAA GACAGCAGAA 
AGCAAAAATG TCCCATTGGA ACGAGAAGCC ACTAGTAAAA ATGACCAAAA TAACTGTCCT 
GTGAAACCCT GTTACCTCAA TATCTTGGAA GATGAGCAAC CTTTAAATAG TGCTGCCCAT 
AAGGAGTCAC CTCCTACTGT TGATTCAACT CAACAGCCTA ACCCTTTGCC GTTACGTTTA 
CCTGAAATGG AACCCTTAGT GCCTAGAGTC AAAGAAGTTA AATCTGCTCA GGAAACTCCT 
GAAAGCTCTC TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA 
GCAGAATCTG GTAATATAAG TCAAAAGTCT GATGAAGAAG ATTTTGTAAA AGTTGAAGAT 
TTACCACTGA AACTGACAAT ATATTCAGAG GCAGATCTAA GAAAGAAAAT GGTAGAAGAA 
GAACAGAAAA ACCATTTATC TGGTGAAATA TGTGAAATGC AGACCGAAGA ATTAGCTGGA 
AATTCTGAGA CACTAAAAGA ACCTGAAACG GTGGGAGCCC AGAGTATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542834.1
CDS	496..6966
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEEASFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPQNDRELRPNRKYSQKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIIVTFFTQGYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_011544532.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544532.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG ATTGTCGATA TAATAGAGAA GGGGAACAGG AGATTCATGT TGCACAAGGT 
GAAGATGATG AGGAGGAGGA GGAAGAAGCA GAAGAGGAGG GAGTCAGTGG AGCTTCATTA 
TCTAGTCACA GGAGCAGTCT GGTTGATGAG CATCCAGAAG ATGCTGAATT TGAACAGAAG 
ATCAACCGAC TTATGGCTGC AAAACAGAAA CTTAGACAGT TACAAGATCT TGTTGCTATG 
GTACAGGATG ATGATGCAGC TCAAGGAGTT ATCTCTGCCA GTGCATCAAA TTTGGATGAT 
TTCTACCCAG CAGAAGAAGA CACCAAGCAA AATTCAAATA ACACTAGAGG AAATGCCAAT 
AAAACACAGA AAGATACTGG AGTAAATGAA AAGGCAAGAG AGAAATTTTA TGAGGCTAAA 
CTACAGCAGC AACAGAGAGA GCTAAAACAA TTGCAGGAAG AAAGAAAGAA ACTGATTGAC 
ATTCAGGAGA AAATTCAAGC ATTGCAAACG GCATGCCCTG ACTTACAGCT GTCAGCTGCT 
AGTGTGGGTA ACTGTCCCAC CAAAAAATAT ATGCCAGCTG TTACTTCAAC CCCAACTGTT 
AATCAACACG AGACCAGTAC AAGCAAATCT GTTTTTGAGC CTGAAGATTC TTCAATAGTA 
GATAATGAGT TGTGGTCAGA AATGAGAAGA CATGAAATGT TGAGGGAGGA GCTGCGACAG 
AGAAGAAAGC AGCTTGAAGC TCTGATGGCT GAACATCAGA GGAGGCAAGG TCTAGCTGAA 
ACTGCATCTC CAGTGGCTGT GTCATTGAGA AGTGATGGAT CTGAGAACCT ATGTACTCCT 
CAGCAAAGTA GAACAGAAAA AACGATGGCA ACTTGGGGAG GGTCTACCCA GTGTGCACTA 
GATGAAGAAG GAGATGAAGA CGGTTACCTT TCTGAAGGAA TTGTTCGGAC AGATGAAGAG 
GAGGAAGAAG AGCAAGATGC CAGTTCCAAT GATAACTTTT CTGTGTGTCC TTCTAACAGT 
GTGAATCATA ACTCCTACAA TGGAAAGGAA ACTAAAAATA GGTGGAAGAA CAATTGCCCT 
TTTTCGGCAG ATGAAAATTA TCGTCCTTTA GCCAAGACAA GGCAACAGAA TATCAGCATG 
CAACGGCAAG AAAACCTTCG TTGGGTGTCA GAGCTCTCTT ACGTAGAAGA GAAAGAACAA 
TGGCAAGAAC AAATCAATCA GCTAAAGAAA CAGCTTGATT TTAGTGTCAG TATTTGTCAG 
ACTTTGATGC AAGACCAGCA GACTCTATCT TGTCTGCTAC AAACTCTTCT CACGGGTCCT 
TACAGTGTTA TGCCCAGCAA TGTTGCATCT CCTCAAGTAC ACTTCATAAT GCACCAGTTG 
AACCAGTGCT ATACTCAGCT AACATGGCAA CAGAATAATG TTCAGAGGTT GAAACAAATG 
CTAAATGAAC TTATGCGCCA GCAAAATCAG CATCCAGAAA AACCTGGAGG CAAGGAAAGA 
GGCAGTAGTG CATCGCACCC TCCTTCTCCC AGTTTATTTT GTCCTTTCAG CTTTCCAACA 
CAGCCTGTAA ATCTCTTCAA TATACCTGGA TTTACTAACT TTTCATCATT TGCACCAGGT 
ATGAATTTCA GCCCTTTATT TCCTTCTAAT TTTGGAGATT TTTCTCAGAA TATCTCTACA 
CCCAGTGAAC AGCAGCAACC CTTAGCCCAG AATTCTTCAG GAAAAACAGA ATATATGGCT 
TTTCCAAAAC CTTTTGAAAG CAGTTCCTCT ATTGGAGCAG AGAAACCAAG GAATAAAAAA 
CTGCCTGAAG AGGAGGTGGA AAGCAGTAGG ACACCATGGT TATATGAACA AGAAGGTGAA 
GTAGAGAAAC CATTTATCAA GACTGGATTT TCAGTGTCTG TAGAAAAATC TACAAGTAGT 
AACCGCAAAA ATCAATTAGA TACAAACGGA AGAAGACGCC AGTTTGATGA AGAATCACTG 
GAAAGCTTTA GCAGTATGCC TGATCCAGTA GATCCAACAA CAGTGACTAA AACATTCAAG 
ACAAGAAAAG CGTCTGCACA GGCCAGCCTG GCATCTAAAG ATAAAACTCC CAAGTCAAAA 
AGTAAGAAGA GGAATTCTAC TCAGCTGAAA AGCAGAGTTA AAAACATCAT TGTCACTTTT 
TTTACTCAAG GGTATGAAAG TGCCAGTATG TCTAGCACAT GTGAACCTTG CAAAAGTAGG 
AACAGACATT CAGCCCAGAC TGAAGAGCCT GTTCAAGCAA AAGTATTCAG CAGAAAGAAT 
CATGAGCAAC TGGAAAAAAT AATAAAATGT AATAGGTCTA CAGAAATATC TTCAGCACAT 
GCTAGGAGAA TACTACAGCA GTCTAACAGA AATGCATGCA ATGAAGCGCC AGAAACTGGG 
AGTGATTTTT CCATGTTTGA AGCTTTGCGA GATACTATTT ATTCTGAAGT AGCTACATTA 
ATTTCTCAAA ATGAATCTCG TCCACATTTT CTTATTGAAC TCTTCCATGA GCTGCAGCTA 
CTAAACACAG ACTACTTGAG ACAGAGGGCT TTATATGCAT TGCAGGACAT AGTATCCAGA 
CATATTTCTG AGAGCCATGA AAAAGGAGAA AATGTAAAGT CAGTAAACTC TGGTACTTGG 
ATAGCATCAA ACTCAGAACT TACTCCTAGT GAGAGCCTTG CTACTACTGA TGATGAAACT 
TTTGAGAAGA ACTTTGAAAG AGAAACCCAT AAAATAAGTG AGCAAAATGA TGCTGATAAT 
GCTAGTGTCC TGTCTGTATC ATCAAATTTT GAGCCTTTTG CAACAGATGA TCTAGGTAAC 
ACCGTGATTC ACTTAGATCA AGCATTAGCC AGAATGAGAG AATATGAGCG TATGAAGACT 
GAGGCTGAAA GTAACTCAAA TATGAGATGC ACCTGCAGGA TTATTGAGGA TGGAGATGGT 
GCTGGTGCAG GTACTACAGT TAATAATTTA GAAGAAACTC CCGTTATTGA AAATCGTAGT 
TCACAACAAC CTGTAAGTGA AGTTTCTACC ATCCCATGTC CTAGAATTGA TACTCAGCAG 
CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGATTTTTCT 
CAGGAGCACA TGGATGAAGT ATGCTCCTCG CAGCTTCTAA CTTCAGTAAG GCGCATGGTT 
TTGACCCTTA CCCAGCAAAA TGATGAGAGC AAAGAGTTTG TAAAGTTCTT TCATAAACAA 
CTTGGAAGTA TATTACAGGA TTCACTGGCA AAATTTGCTG GCAGAAAACT GAAAGACTGT 
GGAGAAGATC TTCTTGTAGA GATATCTGAA GTGTTGTTCA ATGAATTGGC TTTCTTTAAG 
CTTATGCAAG ATTTGGATAA TAATAGTATA ACTGTTAAAC AGAGATGCAA AAGGAAAATA 
GAAGCAACTG GAGTGATACA ATCTTGTGCC AAAGAGGCTA AAAGGATTCT TGAAGATCAT 
GGCTCACCTG CTGGAGAGAT TGATGATGAA GACAAAGACA AGGATGAAAC TGAAACAGTT 
AAGCAGACTC AAACATCTGA GGTGTATGAT GGTCCCAAAA ATGTAAGATC TGATATTTCT 
GATCAAGAGG AAGATGAAGA AAGTGAAGGA TGTCCAGTGT CTATTAATTT GTCTAAAGCT 
GAAACTCAGG CTTTAACTAA TTATGGAAGT GGAGAAGATG AAAATGAGGA TGAAGAAATG 
GAAGAATTTG AAGAAGGCCC TGTGGATGTC CAGACTTCCC TCCAGGCTAA CACTGAAGCT 
ACTGAAGAAA ATGAACATGA TGAACAGGTC CTACAACGTG ACTTTAAAAA GACAGCAGAA 
AGCAAAAATG TCCCATTGGA ACGAGAAGCC ACTAGTAAAA ATGACCAAAA TAACTGTCCT 
GTGAAACCCT GTTACCTCAA TATCTTGGAA GATGAGCAAC CTTTAAATAG TGCTGCCCAT 
AAGGAGTCAC CTCCTACTGT TGATTCAACT CAACAGCCTA ACCCTTTGCC GTTACGTTTA 
CCTGAAATGG AACCCTTAGT GCCTAGAGTC AAAGAAGTTA AATCTGCTCA GGAAACTCCT 
GAAAGCTCTC TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA 
GCAGAATCTG GTAATATAAG TCAAAAGTCT GATGAAGAAG ATTTTGTAAA AGTTGAAGAT 
TTACCACTGA AACTGACAAT ATATTCAGAG GCAGATCTAA GAAAGAAAAT GGTAGAAGAA 
GAACAGAAAA ACCATTTATC TGGTGAAATA TGTGAAATGC AGACCGAAGA ATTAGCTGGA 
AATTCTGAGA CACTAAAAGA ACCTGAAACG GTGGGAGCCC AGAGTATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_011544533.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542835.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

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CloneID	OHu59525
Clone ID Related Accession (Same CDS sequence)	XM_011544533.2
Accession Version	XM_011544533.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CATTGTCACT 
TTTTTTACTC AAGGGTATGA AAGTGCCAGT ATGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCATGAGC AACTGGAAAA AATAATAAAA TGTAATAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGAGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTGCAG 
CTACTAAACA CAGACTACTT GAGACAGAGG GCTTTATATG CATTGCAGGA CATAGTATCC 
AGACATATTT CTGAGAGCCA TGAAAAAGGA GAAAATGTAA AGTCAGTAAA CTCTGGTACT 
TGGATAGCAT CAAACTCAGA ACTTACTCCT AGTGAGAGCC TTGCTACTAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA AAGAGAAACC CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGTG TCCTGTCTGT ATCATCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACCGTGA TTCACTTAGA TCAAGCATTA GCCAGAATGA GAGAATATGA GCGTATGAAG 
ACTGAGGCTG AAAGTAACTC AAATATGAGA TGCACCTGCA GGATTATTGA GGATGGAGAT 
GGTGCTGGTG CAGGTACTAC AGTTAATAAT TTAGAAGAAA CTCCCGTTAT TGAAAATCGT 
AGTTCACAAC AACCTGTAAG TGAAGTTTCT ACCATCCCAT GTCCTAGAAT TGATACTCAG 
CAGCTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC 
ATGGATGAAG TATGCTCCTC GCAGCTTCTA ACTTCAGTAA GGCGCATGGT TTTGACCCTT 
ACCCAGCAAA ATGATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTC AATGAATTGG CTTTCTTTAA GCTTATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCAACT 
GGAGTGATAC AATCTTGTGC CAAAGAGGCT AAAAGGATTC TTGAAGATCA TGGCTCACCT 
GCTGGAGAGA TTGATGATGA AGACAAAGAC AAGGATGAAA CTGAAACAGT TAAGCAGACT 
CAAACATCTG AGGTGTATGA TGGTCCCAAA AATGTAAGAT CTGATATTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGG ATGTCCAGTG TCTATTAATT TGTCTAAAGC TGAAACTCAG 
GCTTTAACTA ATTATGGAAG TGGAGAAGAT GAAAATGAGG ATGAAGAAAT GGAAGAATTT 
GAAGAAGGCC CTGTGGATGT CCAGACTTCC CTCCAGGCTA ACACTGAAGC TACTGAAGAA 
AATGAACATG ATGAACAGGT CCTACAACGT GACTTTAAAA AGACAGCAGA AAGCAAAAAT 
GTCCCATTGG AACGAGAAGC CACTAGTAAA AATGACCAAA ATAACTGTCC TGTGAAACCC 
TGTTACCTCA ATATCTTGGA AGATGAGCAA CCTTTAAATA GTGCTGCCCA TAAGGAGTCA 
CCTCCTACTG TTGATTCAAC TCAACAGCCT AACCCTTTGC CGTTACGTTT ACCTGAAATG 
GAACCCTTAG TGCCTAGAGT CAAAGAAGTT AAATCTGCTC AGGAAACTCC TGAAAGCTCT 
CTGGCTGGAA GTCCTGATAC TGAATCTCCA GTGTTAGTGA ATGACTATGA AGCAGAATCT 
GGTAATATAA GTCAAAAGTC TGATGAAGAA GATTTTGTAA AAGTTGAAGA TTTACCACTG 
AAACTGACAA TATATTCAGA GGCAGATCTA AGAAAGAAAA TGGTAGAAGA AGAACAGAAA 
AACCATTTAT CTGGTGAAAT ATGTGAAATG CAGACCGAAG AATTAGCTGG AAATTCTGAG 
ACACTAAAAG AACCTGAAAC GGTGGGAGCC CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542835.1
CDS	496..6957
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEEASFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPQNDRELRPNRKYSQKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIIVTFFTQGYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_011544533.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544533.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CATTGTCACT 
TTTTTTACTC AAGGGTATGA AAGTGCCAGT ATGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCATGAGC AACTGGAAAA AATAATAAAA TGTAATAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGAGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTGCAG 
CTACTAAACA CAGACTACTT GAGACAGAGG GCTTTATATG CATTGCAGGA CATAGTATCC 
AGACATATTT CTGAGAGCCA TGAAAAAGGA GAAAATGTAA AGTCAGTAAA CTCTGGTACT 
TGGATAGCAT CAAACTCAGA ACTTACTCCT AGTGAGAGCC TTGCTACTAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA AAGAGAAACC CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGTG TCCTGTCTGT ATCATCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACCGTGA TTCACTTAGA TCAAGCATTA GCCAGAATGA GAGAATATGA GCGTATGAAG 
ACTGAGGCTG AAAGTAACTC AAATATGAGA TGCACCTGCA GGATTATTGA GGATGGAGAT 
GGTGCTGGTG CAGGTACTAC AGTTAATAAT TTAGAAGAAA CTCCCGTTAT TGAAAATCGT 
AGTTCACAAC AACCTGTAAG TGAAGTTTCT ACCATCCCAT GTCCTAGAAT TGATACTCAG 
CAGCTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC 
ATGGATGAAG TATGCTCCTC GCAGCTTCTA ACTTCAGTAA GGCGCATGGT TTTGACCCTT 
ACCCAGCAAA ATGATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTC AATGAATTGG CTTTCTTTAA GCTTATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCAACT 
GGAGTGATAC AATCTTGTGC CAAAGAGGCT AAAAGGATTC TTGAAGATCA TGGCTCACCT 
GCTGGAGAGA TTGATGATGA AGACAAAGAC AAGGATGAAA CTGAAACAGT TAAGCAGACT 
CAAACATCTG AGGTGTATGA TGGTCCCAAA AATGTAAGAT CTGATATTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGG ATGTCCAGTG TCTATTAATT TGTCTAAAGC TGAAACTCAG 
GCTTTAACTA ATTATGGAAG TGGAGAAGAT GAAAATGAGG ATGAAGAAAT GGAAGAATTT 
GAAGAAGGCC CTGTGGATGT CCAGACTTCC CTCCAGGCTA ACACTGAAGC TACTGAAGAA 
AATGAACATG ATGAACAGGT CCTACAACGT GACTTTAAAA AGACAGCAGA AAGCAAAAAT 
GTCCCATTGG AACGAGAAGC CACTAGTAAA AATGACCAAA ATAACTGTCC TGTGAAACCC 
TGTTACCTCA ATATCTTGGA AGATGAGCAA CCTTTAAATA GTGCTGCCCA TAAGGAGTCA 
CCTCCTACTG TTGATTCAAC TCAACAGCCT AACCCTTTGC CGTTACGTTT ACCTGAAATG 
GAACCCTTAG TGCCTAGAGT CAAAGAAGTT AAATCTGCTC AGGAAACTCC TGAAAGCTCT 
CTGGCTGGAA GTCCTGATAC TGAATCTCCA GTGTTAGTGA ATGACTATGA AGCAGAATCT 
GGTAATATAA GTCAAAAGTC TGATGAAGAA GATTTTGTAA AAGTTGAAGA TTTACCACTG 
AAACTGACAA TATATTCAGA GGCAGATCTA AGAAAGAAAA TGGTAGAAGA AGAACAGAAA 
AACCATTTAT CTGGTGAAAT ATGTGAAATG CAGACCGAAG AATTAGCTGG AAATTCTGAG 
ACACTAAAAG AACCTGAAAC GGTGGGAGCC CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_011544534.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542836.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X7, mRNA.

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CloneID	OHu59526
Clone ID Related Accession (Same CDS sequence)	XM_011544534.2
Accession Version	XM_011544534.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544534.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CATTGTCACT 
TTTTTTACTC AAGGGTATGA AAGTGCCAGT ATGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCATGAGC AACTGGAAAA AATAATAAAA TGTAATAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGAGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTGCAG 
CTACTAAACA CAGACTACTT GAGACAGAGG GCTTTATATG CATTGCAGGA CATAGTATCC 
AGACATATTT CTGAGAGCCA TGAAAAAGGA GAAAATGTAA AGTCAGTAAA CTCTGGTACT 
TGGATAGCAT CAAACTCAGA ACTTACTCCT AGTGAGAGCC TTGCTACTAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA AAGAGAAACC CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGTG TCCTGTCTGT ATCATCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACCGTGA TTCACTTAGA TCAAGCATTA GCCAGAATGA GAGAATATGA GCGTATGAAG 
ACTGAGGCTG AAAGTAACTC AAATATGAGA TGCACCTGCA GGATTATTGA GGATGGAGAT 
GGTGCTGGTG CAGAAACTCC CGTTATTGAA AATCGTAGTT CACAACAACC TGTAAGTGAA 
GTTTCTACCA TCCCATGTCC TAGAATTGAT ACTCAGCAGC TGGACCGGCA AATTAAAGCA 
ATTATGAAAG AAGTCATTCC TTTTTTGAAG GATTTTTCTC AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542836.1
CDS	496..6945
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEEASFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPQNDRELRPNRKYSQKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIIVTFFTQGYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_011544534.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544534.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CATTGTCACT 
TTTTTTACTC AAGGGTATGA AAGTGCCAGT ATGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCATGAGC AACTGGAAAA AATAATAAAA TGTAATAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGAGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTGCAG 
CTACTAAACA CAGACTACTT GAGACAGAGG GCTTTATATG CATTGCAGGA CATAGTATCC 
AGACATATTT CTGAGAGCCA TGAAAAAGGA GAAAATGTAA AGTCAGTAAA CTCTGGTACT 
TGGATAGCAT CAAACTCAGA ACTTACTCCT AGTGAGAGCC TTGCTACTAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA AAGAGAAACC CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGTG TCCTGTCTGT ATCATCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACCGTGA TTCACTTAGA TCAAGCATTA GCCAGAATGA GAGAATATGA GCGTATGAAG 
ACTGAGGCTG AAAGTAACTC AAATATGAGA TGCACCTGCA GGATTATTGA GGATGGAGAT 
GGTGCTGGTG CAGAAACTCC CGTTATTGAA AATCGTAGTT CACAACAACC TGTAAGTGAA 
GTTTCTACCA TCCCATGTCC TAGAATTGAT ACTCAGCAGC TGGACCGGCA AATTAAAGCA 
ATTATGAAAG AAGTCATTCC TTTTTTGAAG GATTTTTCTC AGGAGCACAT GGATGAAGTA 
TGCTCCTCGC AGCTTCTAAC TTCAGTAAGG CGCATGGTTT TGACCCTTAC CCAGCAAAAT 
GATGAGAGCA AAGAGTTTGT AAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTCAA TGAATTGGCT TTCTTTAAGC TTATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAGATGCAAA AGGAAAATAG AAGCAACTGG AGTGATACAA 
TCTTGTGCCA AAGAGGCTAA AAGGATTCTT GAAGATCATG GCTCACCTGC TGGAGAGATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCAGACTCA AACATCTGAG 
GTGTATGATG GTCCCAAAAA TGTAAGATCT GATATTTCTG ATCAAGAGGA AGATGAAGAA 
AGTGAAGGAT GTCCAGTGTC TATTAATTTG TCTAAAGCTG AAACTCAGGC TTTAACTAAT 
TATGGAAGTG GAGAAGATGA AAATGAGGAT GAAGAAATGG AAGAATTTGA AGAAGGCCCT 
GTGGATGTCC AGACTTCCCT CCAGGCTAAC ACTGAAGCTA CTGAAGAAAA TGAACATGAT 
GAACAGGTCC TACAACGTGA CTTTAAAAAG ACAGCAGAAA GCAAAAATGT CCCATTGGAA 
CGAGAAGCCA CTAGTAAAAA TGACCAAAAT AACTGTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAG ATGAGCAACC TTTAAATAGT GCTGCCCATA AGGAGTCACC TCCTACTGTT 
GATTCAACTC AACAGCCTAA CCCTTTGCCG TTACGTTTAC CTGAAATGGA ACCCTTAGTG 
CCTAGAGTCA AAGAAGTTAA ATCTGCTCAG GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAATATAAGT 
CAAAAGTCTG ATGAAGAAGA TTTTGTAAAA GTTGAAGATT TACCACTGAA ACTGACAATA 
TATTCAGAGG CAGATCTAAG AAAGAAAATG GTAGAAGAAG AACAGAAAAA CCATTTATCT 
GGTGAAATAT GTGAAATGCA GACCGAAGAA TTAGCTGGAA ATTCTGAGAC ACTAAAAGAA 
CCTGAAACGG TGGGAGCCCA GAGTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_011544535.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542837.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X9, mRNA.

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CloneID	OHu59527
Clone ID Related Accession (Same CDS sequence)	XM_011544535.2
Accession Version	XM_011544535.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CATTGTCACT 
TTTTTTACTC AAGGGTATGA AAGTGCCAGT ATGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCATGAGC AACTGGAAAA AATAATAAAA TGTAATAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGAGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTGCAG 
CTACTAAACA CAGACTACTT GAGACAGAGG GCTTTATATG CATTGCAGGA CATAGTATCC 
AGACATATTT CTGAGAGCCA TGAAAAAGGA GAAAATGTAA AGTCAGTAAA CTCTGGTACT 
TGGATAGCAT CAAACTCAGA ACTTACTCCT AGTGAGAGCC TTGCTACTAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA AAGAGAAACC CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGTG TCCTGTCTGT ATCATCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACCGTGA TTCACTTAGA TCAAGCATTA GCCAGAATGA GAGAATATGA GCGTATGAAG 
ACTGAGGCTG AAAGTAACTC AAATATGAGA TGCACCTGCA GGATTATTGA GGATGGAGAT 
GGTGCTGGTG CAGAAACTCC CGTTATTGAA AATCGTAGTT CACAACAACC TGTAAGTGAA 
GTTTCTACCA TCCCATGTCC TAGAATTGAT ACTCAGCAGC TGGACCGGCA AATTAAAGCA 
ATTATGAAAG AAGTCATTCC TTTTTTGAAG GAGCACATGG ATGAAGTATG CTCCTCGCAG 
CTTCTAACTT CAGTAAGGCG CATGGTTTTG ACCCTTACCC AGCAAAATGA TGAGAGCAAA 
GAGTTTGTAA AGTTCTTTCA TAAACAACTT GGAAGTATAT TACAGGATTC ACTGGCAAAA 
TTTGCTGGCA GAAAACTGAA AGACTGTGGA GAAGATCTTC TTGTAGAGAT ATCTGAAGTG 
TTGTTCAATG AATTGGCTTT CTTTAAGCTT ATGCAAGATT TGGATAATAA TAGTATAACT 
GTTAAACAGA GATGCAAAAG GAAAATAGAA GCAACTGGAG TGATACAATC TTGTGCCAAA 
GAGGCTAAAA GGATTCTTGA AGATCATGGC TCACCTGCTG GAGAGATTGA TGATGAAGAC 
AAAGACAAGG ATGAAACTGA AACAGTTAAG CAGACTCAAA CATCTGAGGT GTATGATGGT 
CCCAAAAATG TAAGATCTGA TATTTCTGAT CAAGAGGAAG ATGAAGAAAG TGAAGGATGT 
CCAGTGTCTA TTAATTTGTC TAAAGCTGAA ACTCAGGCTT TAACTAATTA TGGAAGTGGA 
GAAGATGAAA ATGAGGATGA AGAAATGGAA GAATTTGAAG AAGGCCCTGT GGATGTCCAG 
ACTTCCCTCC AGGCTAACAC TGAAGCTACT GAAGAAAATG AACATGATGA ACAGGTCCTA 
CAACGTGACT TTAAAAAGAC AGCAGAAAGC AAAAATGTCC CATTGGAACG AGAAGCCACT 
AGTAAAAATG ACCAAAATAA CTGTCCTGTG AAACCCTGTT ACCTCAATAT CTTGGAAGAT 
GAGCAACCTT TAAATAGTGC TGCCCATAAG GAGTCACCTC CTACTGTTGA TTCAACTCAA 
CAGCCTAACC CTTTGCCGTT ACGTTTACCT GAAATGGAAC CCTTAGTGCC TAGAGTCAAA 
GAAGTTAAAT CTGCTCAGGA AACTCCTGAA AGCTCTCTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ATATAAGTCA AAAGTCTGAT 
GAAGAAGATT TTGTAAAAGT TGAAGATTTA CCACTGAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGAA AGAAAATGGT AGAAGAAGAA CAGAAAAACC ATTTATCTGG TGAAATATGT 
GAAATGCAGA CCGAAGAATT AGCTGGAAAT TCTGAGACAC TAAAAGAACC TGAAACGGTG 
GGAGCCCAGA GTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542837.1
CDS	496..6933
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEEASFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPQNDRELRPNRKYSQKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIIVTFFTQGYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_011544535.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544535.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAGGCAT CTTTTAGTCT GAGAATTCGG CCGTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTTC AGACATTGAC GTTACTACAA GTCCAAAAGG GAAAGGTGAC 
AGACCTCAGA ATGACAGGGA ACTGAGGCCT AATAGGAAAT ATAGCCAAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAACATGATT TATTAAAAAG AATGTTACAA CAGCAGGAGC AACTAAGAGC TCTACAGGGA 
CGGCAGGCTG CACTTCTAGC TCTGCAACAT AAAGCAGAGC AAGCTATTGC AGTGATGGAT 
GATTCTGTTG TTGCAGAAAC TGCAGGTAGC TTATCTGGCG TCAGTATCAC ATCTGAACTA 
AATGAAGAAT TGAATGACTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAGCTGTTC CAGACAATAG AAGACAGGCA GAAAGTCTTT CATTAACTAG GGAGGTTTCC 
CAGAGCAGGA AACCATCAGC TTCAGAACGT TTACCTGATG AGAAAGTCGA ACTTTTTAGC 
AAAATGAGAG TGCTACAGGA AAAGAAACAA AAAATGGACA AATTGCTTGG AGAACTTCAT 
ACACTTCGAG ATCAGCATCT TAACAATTCA TCATCCTCTC CACAAAGGAG TGTCGATCAG 
AGAAGTACTT CAGCTCCCTC TGCTTCTGTA GGCTTGGCAC CGGTTGTCAA TGGAGAATCC 
AATAGCCTCA CATCATCTGT TCCTTATCCT ACTGCTTCTC TAGTATCTCA GAATGAGAGT 
GAAAACGAAG GCCACCTCAA TCCATCTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG 
AGATTGAATG AGCTAAGAGA ATTAGTTCAT TATTATGAAC AAACGTCAGA CATGATGACA 
GATGCTGTGA ATGAAAACAG GAAAGATGAA GAAACTGAAG AGTCAGAATA TGATTCTGAG 
CATGAAAATT CCGAGCCTGT TACTAACATT CGAAATCCAC AAGTAGCTTC CACTTGGAAT 
GAAGTAAATA GTCATAGTAA TGCACAGTGT GTTTCTAATA ATAGAGATGG GCGAACAGTT 
AATTCTAATT GTGAAATTAA CAACAGATCT GCTGCCAACA TAAGGGCTCT AAACATGCCT 
CCTTCTTTAG CAGATTGTCG ATATAATAGA GAAGGGGAAC AGGAGATTCA TGTTGCACAA 
GGTGAAGATG ATGAGGAGGA GGAGGAAGAA GCAGAAGAGG AGGGAGTCAG TGGAGCTTCA 
TTATCTAGTC ACAGGAGCAG TCTGGTTGAT GAGCATCCAG AAGATGCTGA ATTTGAACAG 
AAGATCAACC GACTTATGGC TGCAAAACAG AAACTTAGAC AGTTACAAGA TCTTGTTGCT 
ATGGTACAGG ATGATGATGC AGCTCAAGGA GTTATCTCTG CCAGTGCATC AAATTTGGAT 
GATTTCTACC CAGCAGAAGA AGACACCAAG CAAAATTCAA ATAACACTAG AGGAAATGCC 
AATAAAACAC AGAAAGATAC TGGAGTAAAT GAAAAGGCAA GAGAGAAATT TTATGAGGCT 
AAACTACAGC AGCAACAGAG AGAGCTAAAA CAATTGCAGG AAGAAAGAAA GAAACTGATT 
GACATTCAGG AGAAAATTCA AGCATTGCAA ACGGCATGCC CTGACTTACA GCTGTCAGCT 
GCTAGTGTGG GTAACTGTCC CACCAAAAAA TATATGCCAG CTGTTACTTC AACCCCAACT 
GTTAATCAAC ACGAGACCAG TACAAGCAAA TCTGTTTTTG AGCCTGAAGA TTCTTCAATA 
GTAGATAATG AGTTGTGGTC AGAAATGAGA AGACATGAAA TGTTGAGGGA GGAGCTGCGA 
CAGAGAAGAA AGCAGCTTGA AGCTCTGATG GCTGAACATC AGAGGAGGCA AGGTCTAGCT 
GAAACTGCAT CTCCAGTGGC TGTGTCATTG AGAAGTGATG GATCTGAGAA CCTATGTACT 
CCTCAGCAAA GTAGAACAGA AAAAACGATG GCAACTTGGG GAGGGTCTAC CCAGTGTGCA 
CTAGATGAAG AAGGAGATGA AGACGGTTAC CTTTCTGAAG GAATTGTTCG GACAGATGAA 
GAGGAGGAAG AAGAGCAAGA TGCCAGTTCC AATGATAACT TTTCTGTGTG TCCTTCTAAC 
AGTGTGAATC ATAACTCCTA CAATGGAAAG GAAACTAAAA ATAGGTGGAA GAACAATTGC 
CCTTTTTCGG CAGATGAAAA TTATCGTCCT TTAGCCAAGA CAAGGCAACA GAATATCAGC 
ATGCAACGGC AAGAAAACCT TCGTTGGGTG TCAGAGCTCT CTTACGTAGA AGAGAAAGAA 
CAATGGCAAG AACAAATCAA TCAGCTAAAG AAACAGCTTG ATTTTAGTGT CAGTATTTGT 
CAGACTTTGA TGCAAGACCA GCAGACTCTA TCTTGTCTGC TACAAACTCT TCTCACGGGT 
CCTTACAGTG TTATGCCCAG CAATGTTGCA TCTCCTCAAG TACACTTCAT AATGCACCAG 
TTGAACCAGT GCTATACTCA GCTAACATGG CAACAGAATA ATGTTCAGAG GTTGAAACAA 
ATGCTAAATG AACTTATGCG CCAGCAAAAT CAGCATCCAG AAAAACCTGG AGGCAAGGAA 
AGAGGCAGTA GTGCATCGCA CCCTCCTTCT CCCAGTTTAT TTTGTCCTTT CAGCTTTCCA 
ACACAGCCTG TAAATCTCTT CAATATACCT GGATTTACTA ACTTTTCATC ATTTGCACCA 
GGTATGAATT TCAGCCCTTT ATTTCCTTCT AATTTTGGAG ATTTTTCTCA GAATATCTCT 
ACACCCAGTG AACAGCAGCA ACCCTTAGCC CAGAATTCTT CAGGAAAAAC AGAATATATG 
GCTTTTCCAA AACCTTTTGA AAGCAGTTCC TCTATTGGAG CAGAGAAACC AAGGAATAAA 
AAACTGCCTG AAGAGGAGGT GGAAAGCAGT AGGACACCAT GGTTATATGA ACAAGAAGGT 
GAAGTAGAGA AACCATTTAT CAAGACTGGA TTTTCAGTGT CTGTAGAAAA ATCTACAAGT 
AGTAACCGCA AAAATCAATT AGATACAAAC GGAAGAAGAC GCCAGTTTGA TGAAGAATCA 
CTGGAAAGCT TTAGCAGTAT GCCTGATCCA GTAGATCCAA CAACAGTGAC TAAAACATTC 
AAGACAAGAA AAGCGTCTGC ACAGGCCAGC CTGGCATCTA AAGATAAAAC TCCCAAGTCA 
AAAAGTAAGA AGAGGAATTC TACTCAGCTG AAAAGCAGAG TTAAAAACAT CATTGTCACT 
TTTTTTACTC AAGGGTATGA AAGTGCCAGT ATGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCATGAGC AACTGGAAAA AATAATAAAA TGTAATAGGT CTACAGAAAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGAGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTGCAG 
CTACTAAACA CAGACTACTT GAGACAGAGG GCTTTATATG CATTGCAGGA CATAGTATCC 
AGACATATTT CTGAGAGCCA TGAAAAAGGA GAAAATGTAA AGTCAGTAAA CTCTGGTACT 
TGGATAGCAT CAAACTCAGA ACTTACTCCT AGTGAGAGCC TTGCTACTAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA AAGAGAAACC CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGTG TCCTGTCTGT ATCATCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACCGTGA TTCACTTAGA TCAAGCATTA GCCAGAATGA GAGAATATGA GCGTATGAAG 
ACTGAGGCTG AAAGTAACTC AAATATGAGA TGCACCTGCA GGATTATTGA GGATGGAGAT 
GGTGCTGGTG CAGAAACTCC CGTTATTGAA AATCGTAGTT CACAACAACC TGTAAGTGAA 
GTTTCTACCA TCCCATGTCC TAGAATTGAT ACTCAGCAGC TGGACCGGCA AATTAAAGCA 
ATTATGAAAG AAGTCATTCC TTTTTTGAAG GAGCACATGG ATGAAGTATG CTCCTCGCAG 
CTTCTAACTT CAGTAAGGCG CATGGTTTTG ACCCTTACCC AGCAAAATGA TGAGAGCAAA 
GAGTTTGTAA AGTTCTTTCA TAAACAACTT GGAAGTATAT TACAGGATTC ACTGGCAAAA 
TTTGCTGGCA GAAAACTGAA AGACTGTGGA GAAGATCTTC TTGTAGAGAT ATCTGAAGTG 
TTGTTCAATG AATTGGCTTT CTTTAAGCTT ATGCAAGATT TGGATAATAA TAGTATAACT 
GTTAAACAGA GATGCAAAAG GAAAATAGAA GCAACTGGAG TGATACAATC TTGTGCCAAA 
GAGGCTAAAA GGATTCTTGA AGATCATGGC TCACCTGCTG GAGAGATTGA TGATGAAGAC 
AAAGACAAGG ATGAAACTGA AACAGTTAAG CAGACTCAAA CATCTGAGGT GTATGATGGT 
CCCAAAAATG TAAGATCTGA TATTTCTGAT CAAGAGGAAG ATGAAGAAAG TGAAGGATGT 
CCAGTGTCTA TTAATTTGTC TAAAGCTGAA ACTCAGGCTT TAACTAATTA TGGAAGTGGA 
GAAGATGAAA ATGAGGATGA AGAAATGGAA GAATTTGAAG AAGGCCCTGT GGATGTCCAG 
ACTTCCCTCC AGGCTAACAC TGAAGCTACT GAAGAAAATG AACATGATGA ACAGGTCCTA 
CAACGTGACT TTAAAAAGAC AGCAGAAAGC AAAAATGTCC CATTGGAACG AGAAGCCACT 
AGTAAAAATG ACCAAAATAA CTGTCCTGTG AAACCCTGTT ACCTCAATAT CTTGGAAGAT 
GAGCAACCTT TAAATAGTGC TGCCCATAAG GAGTCACCTC CTACTGTTGA TTCAACTCAA 
CAGCCTAACC CTTTGCCGTT ACGTTTACCT GAAATGGAAC CCTTAGTGCC TAGAGTCAAA 
GAAGTTAAAT CTGCTCAGGA AACTCCTGAA AGCTCTCTGG CTGGAAGTCC TGATACTGAA 
TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ATATAAGTCA AAAGTCTGAT 
GAAGAAGATT TTGTAAAAGT TGAAGATTTA CCACTGAAAC TGACAATATA TTCAGAGGCA 
GATCTAAGAA AGAAAATGGT AGAAGAAGAA CAGAAAAACC ATTTATCTGG TGAAATATGT 
GAAATGCAGA CCGAAGAATT AGCTGGAAAT TCTGAGACAC TAAAAGAACC TGAAACGGTG 
GGAGCCCAGA GTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_011544540.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542842.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X12, mRNA.

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CloneID	OHu59532
Clone ID Related Accession (Same CDS sequence)	XM_011544540.2 , XM_017013473.1
Accession Version	XM_011544540.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA TTGTCACTTT TTTTACTCAA 
GGGTATGAAA GTGCCAGTAT GTCTAGCACA TGTGAACCTT GCAAAAGTAG GAACAGACAT 
TCAGCCCAGA CTGAAGAGCC TGTTCAAGCA AAAGTATTCA GCAGAAAGAA TCATGAGCAA 
CTGGAAAAAA TAATAAAATG TAATAGGTCT ACAGAAATAT CTTCAGCACA TGCTAGGAGA 
ATACTACAGC AGTCTAACAG AAATGCATGC AATGAAGCGC CAGAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGATTTTTC TCAGGAGCAC 
ATGGATGAAG TATGCTCCTC GCAGCTTCTA ACTTCAGTAA GGCGCATGGT TTTGACCCTT 
ACCCAGCAAA ATGATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTC AATGAATTGG CTTTCTTTAA GCTTATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCAACT 
GGAGTGATAC AATCTTGTGC CAAAGAGGCT AAAAGGATTC TTGAAGATCA TGGCTCACCT 
GCTGGAGAGA TTGATGATGA AGACAAAGAC AAGGATGAAA CTGAAACAGT TAAGCAGACT 
CAAACATCTG AGGTGTATGA TGGTCCCAAA AATGTAAGAT CTGATATTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGG ATGTCCAGTG TCTATTAATT TGTCTAAAGC TGAAACTCAG 
GCTTTAACTA ATTATGGAAG TGGAGAAGAT GAAAATGAGG ATGAAGAAAT GGAAGAATTT 
GAAGAAGGCC CTGTGGATGT CCAGACTTCC CTCCAGGCTA ACACTGAAGC TACTGAAGAA 
AATGAACATG ATGAACAGGT CCTACAACGT GACTTTAAAA AGACAGCAGA AAGCAAAAAT 
GTCCCATTGG AACGAGAAGC CACTAGTAAA AATGACCAAA ATAACTGTCC TGTGAAACCC 
TGTTACCTCA ATATCTTGGA AGATGAGCAA CCTTTAAATA GTGCTGCCCA TAAGGAGTCA 
CCTCCTACTG TTGATTCAAC TCAACAGCCT AACCCTTTGC CGTTACGTTT ACCTGAAATG 
GAACCCTTAG TGCCTAGAGT CAAAGAAGTT AAATCTGCTC AGGAAACTCC TGAAAGCTCT 
CTGGCTGGAA GTCCTGATAC TGAATCTCCA GTGTTAGTGA ATGACTATGA AGCAGAATCT 
GGTAATATAA GTCAAAAGTC TGATGAAGAA GATTTTGTAA AAGTTGAAGA TTTACCACTG 
AAACTGACAA TATATTCAGA GGCAGATCTA AGAAAGAAAA TGGTAGAAGA AGAACAGAAA 
AACCATTTAT CTGGTGAAAT ATGTGAAATG CAGACCGAAG AATTAGCTGG AAATTCTGAG 
ACACTAAAAG AACCTGAAAC GGTGGGAGCC CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542842.1
CDS	616..6897
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIIVTFFTQGYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_011544540.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544540.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA TTGTCACTTT TTTTACTCAA 
GGGTATGAAA GTGCCAGTAT GTCTAGCACA TGTGAACCTT GCAAAAGTAG GAACAGACAT 
TCAGCCCAGA CTGAAGAGCC TGTTCAAGCA AAAGTATTCA GCAGAAAGAA TCATGAGCAA 
CTGGAAAAAA TAATAAAATG TAATAGGTCT ACAGAAATAT CTTCAGCACA TGCTAGGAGA 
ATACTACAGC AGTCTAACAG AAATGCATGC AATGAAGCGC CAGAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGATTTTTC TCAGGAGCAC 
ATGGATGAAG TATGCTCCTC GCAGCTTCTA ACTTCAGTAA GGCGCATGGT TTTGACCCTT 
ACCCAGCAAA ATGATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTC AATGAATTGG CTTTCTTTAA GCTTATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCAACT 
GGAGTGATAC AATCTTGTGC CAAAGAGGCT AAAAGGATTC TTGAAGATCA TGGCTCACCT 
GCTGGAGAGA TTGATGATGA AGACAAAGAC AAGGATGAAA CTGAAACAGT TAAGCAGACT 
CAAACATCTG AGGTGTATGA TGGTCCCAAA AATGTAAGAT CTGATATTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGG ATGTCCAGTG TCTATTAATT TGTCTAAAGC TGAAACTCAG 
GCTTTAACTA ATTATGGAAG TGGAGAAGAT GAAAATGAGG ATGAAGAAAT GGAAGAATTT 
GAAGAAGGCC CTGTGGATGT CCAGACTTCC CTCCAGGCTA ACACTGAAGC TACTGAAGAA 
AATGAACATG ATGAACAGGT CCTACAACGT GACTTTAAAA AGACAGCAGA AAGCAAAAAT 
GTCCCATTGG AACGAGAAGC CACTAGTAAA AATGACCAAA ATAACTGTCC TGTGAAACCC 
TGTTACCTCA ATATCTTGGA AGATGAGCAA CCTTTAAATA GTGCTGCCCA TAAGGAGTCA 
CCTCCTACTG TTGATTCAAC TCAACAGCCT AACCCTTTGC CGTTACGTTT ACCTGAAATG 
GAACCCTTAG TGCCTAGAGT CAAAGAAGTT AAATCTGCTC AGGAAACTCC TGAAAGCTCT 
CTGGCTGGAA GTCCTGATAC TGAATCTCCA GTGTTAGTGA ATGACTATGA AGCAGAATCT 
GGTAATATAA GTCAAAAGTC TGATGAAGAA GATTTTGTAA AAGTTGAAGA TTTACCACTG 
AAACTGACAA TATATTCAGA GGCAGATCTA AGAAAGAAAA TGGTAGAAGA AGAACAGAAA 
AACCATTTAT CTGGTGAAAT ATGTGAAATG CAGACCGAAG AATTAGCTGG AAATTCTGAG 
ACACTAAAAG AACCTGAAAC GGTGGGAGCC CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013473.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868962.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X10, mRNA.

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CloneID	OHu59532
Clone ID Related Accession (Same CDS sequence)	XM_011544540.2 , XM_017013473.1
Accession Version	XM_017013473.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA TTGTCACTTT TTTTACTCAA 
GGGTATGAAA GTGCCAGTAT GTCTAGCACA TGTGAACCTT GCAAAAGTAG GAACAGACAT 
TCAGCCCAGA CTGAAGAGCC TGTTCAAGCA AAAGTATTCA GCAGAAAGAA TCATGAGCAA 
CTGGAAAAAA TAATAAAATG TAATAGGTCT ACAGAAATAT CTTCAGCACA TGCTAGGAGA 
ATACTACAGC AGTCTAACAG AAATGCATGC AATGAAGCGC CAGAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGATTTTTC TCAGGAGCAC 
ATGGATGAAG TATGCTCCTC GCAGCTTCTA ACTTCAGTAA GGCGCATGGT TTTGACCCTT 
ACCCAGCAAA ATGATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTC AATGAATTGG CTTTCTTTAA GCTTATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCAACT 
GGAGTGATAC AATCTTGTGC CAAAGAGGCT AAAAGGATTC TTGAAGATCA TGGCTCACCT 
GCTGGAGAGA TTGATGATGA AGACAAAGAC AAGGATGAAA CTGAAACAGT TAAGCAGACT 
CAAACATCTG AGGTGTATGA TGGTCCCAAA AATGTAAGAT CTGATATTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGG ATGTCCAGTG TCTATTAATT TGTCTAAAGC TGAAACTCAG 
GCTTTAACTA ATTATGGAAG TGGAGAAGAT GAAAATGAGG ATGAAGAAAT GGAAGAATTT 
GAAGAAGGCC CTGTGGATGT CCAGACTTCC CTCCAGGCTA ACACTGAAGC TACTGAAGAA 
AATGAACATG ATGAACAGGT CCTACAACGT GACTTTAAAA AGACAGCAGA AAGCAAAAAT 
GTCCCATTGG AACGAGAAGC CACTAGTAAA AATGACCAAA ATAACTGTCC TGTGAAACCC 
TGTTACCTCA ATATCTTGGA AGATGAGCAA CCTTTAAATA GTGCTGCCCA TAAGGAGTCA 
CCTCCTACTG TTGATTCAAC TCAACAGCCT AACCCTTTGC CGTTACGTTT ACCTGAAATG 
GAACCCTTAG TGCCTAGAGT CAAAGAAGTT AAATCTGCTC AGGAAACTCC TGAAAGCTCT 
CTGGCTGGAA GTCCTGATAC TGAATCTCCA GTGTTAGTGA ATGACTATGA AGCAGAATCT 
GGTAATATAA GTCAAAAGTC TGATGAAGAA GATTTTGTAA AAGTTGAAGA TTTACCACTG 
AAACTGACAA TATATTCAGA GGCAGATCTA AGAAAGAAAA TGGTAGAAGA AGAACAGAAA 
AACCATTTAT CTGGTGAAAT ATGTGAAATG CAGACCGAAG AATTAGCTGG AAATTCTGAG 
ACACTAAAAG AACCTGAAAC GGTGGGAGCC CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868962.1
CDS	800..7081
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIIVTFFTQGYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013473.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013473.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT 
TTATTAAAAA GAATGTTACA ACAGCAGGAG CAACTAAGAG CTCTACAGGG ACGGCAGGCT 
GCACTTCTAG CTCTGCAACA TAAAGCAGAG CAAGCTATTG CAGTGATGGA TGATTCTGTT 
GTTGCAGAAA CTGCAGGTAG CTTATCTGGC GTCAGTATCA CATCTGAACT AAATGAAGAA 
TTGAATGACT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT 
CCAGACAATA GAAGACAGGC AGAAAGTCTT TCATTAACTA GGGAGGTTTC CCAGAGCAGG 
AAACCATCAG CTTCAGAACG TTTACCTGAT GAGAAAGTCG AACTTTTTAG CAAAATGAGA 
GTGCTACAGG AAAAGAAACA AAAAATGGAC AAATTGCTTG GAGAACTTCA TACACTTCGA 
GATCAGCATC TTAACAATTC ATCATCCTCT CCACAAAGGA GTGTCGATCA GAGAAGTACT 
TCAGCTCCCT CTGCTTCTGT AGGCTTGGCA CCGGTTGTCA ATGGAGAATC CAATAGCCTC 
ACATCATCTG TTCCTTATCC TACTGCTTCT CTAGTATCTC AGAATGAGAG TGAAAACGAA 
GGCCACCTCA ATCCATCTGA AAAACTCCAG AAGTTAAATG AAGTTCGAAA GAGATTGAAT 
GAGCTAAGAG AATTAGTTCA TTATTATGAA CAAACGTCAG ACATGATGAC AGATGCTGTG 
AATGAAAACA GGAAAGATGA AGAAACTGAA GAGTCAGAAT ATGATTCTGA GCATGAAAAT 
TCCGAGCCTG TTACTAACAT TCGAAATCCA CAAGTAGCTT CCACTTGGAA TGAAGTAAAT 
AGTCATAGTA ATGCACAGTG TGTTTCTAAT AATAGAGATG GGCGAACAGT TAATTCTAAT 
TGTGAAATTA ACAACAGATC TGCTGCCAAC ATAAGGGCTC TAAACATGCC TCCTTCTTTA 
GATTGTCGAT ATAATAGAGA AGGGGAACAG GAGATTCATG TTGCACAAGG TGAAGATGAT 
GAGGAGGAGG AGGAAGAAGC AGAAGAGGAG GGAGTCAGTG GAGCTTCATT ATCTAGTCAC 
AGGAGCAGTC TGGTTGATGA GCATCCAGAA GATGCTGAAT TTGAACAGAA GATCAACCGA 
CTTATGGCTG CAAAACAGAA ACTTAGACAG TTACAAGATC TTGTTGCTAT GGTACAGGAT 
GATGATGCAG CTCAAGGAGT TATCTCTGCC AGTGCATCAA ATTTGGATGA TTTCTACCCA 
GCAGAAGAAG ACACCAAGCA AAATTCAAAT AACACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATACTG GAGTAAATGA AAAGGCAAGA GAGAAATTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTAAAACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA CATTCAGGAG 
AAAATTCAAG CATTGCAAAC GGCATGCCCT GACTTACAGC TGTCAGCTGC TAGTGTGGGT 
AACTGTCCCA CCAAAAAATA TATGCCAGCT GTTACTTCAA CCCCAACTGT TAATCAACAC 
GAGACCAGTA CAAGCAAATC TGTTTTTGAG CCTGAAGATT CTTCAATAGT AGATAATGAG 
TTGTGGTCAG AAATGAGAAG ACATGAAATG TTGAGGGAGG AGCTGCGACA GAGAAGAAAG 
CAGCTTGAAG CTCTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTGCATCT 
CCAGTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TATGTACTCC TCAGCAAAGT 
AGAACAGAAA AAACGATGGC AACTTGGGGA GGGTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ACGGTTACCT TTCTGAAGGA ATTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAGCAAGATG CCAGTTCCAA TGATAACTTT TCTGTGTGTC CTTCTAACAG TGTGAATCAT 
AACTCCTACA ATGGAAAGGA AACTAAAAAT AGGTGGAAGA ACAATTGCCC TTTTTCGGCA 
GATGAAAATT ATCGTCCTTT AGCCAAGACA AGGCAACAGA ATATCAGCAT GCAACGGCAA 
GAAAACCTTC GTTGGGTGTC AGAGCTCTCT TACGTAGAAG AGAAAGAACA ATGGCAAGAA 
CAAATCAATC AGCTAAAGAA ACAGCTTGAT TTTAGTGTCA GTATTTGTCA GACTTTGATG 
CAAGACCAGC AGACTCTATC TTGTCTGCTA CAAACTCTTC TCACGGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTTGCATC TCCTCAAGTA CACTTCATAA TGCACCAGTT GAACCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTTCAGAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AGCAAAATCA GCATCCAGAA AAACCTGGAG GCAAGGAAAG AGGCAGTAGT 
GCATCGCACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCAAC ACAGCCTGTA 
AATCTCTTCA ATATACCTGG ATTTACTAAC TTTTCATCAT TTGCACCAGG TATGAATTTC 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAGA ATATCTCTAC ACCCAGTGAA 
CAGCAGCAAC CCTTAGCCCA GAATTCTTCA GGAAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCCTC TATTGGAGCA GAGAAACCAA GGAATAAAAA ACTGCCTGAA 
GAGGAGGTGG AAAGCAGTAG GACACCATGG TTATATGAAC AAGAAGGTGA AGTAGAGAAA 
CCATTTATCA AGACTGGATT TTCAGTGTCT GTAGAAAAAT CTACAAGTAG TAACCGCAAA 
AATCAATTAG ATACAAACGG AAGAAGACGC CAGTTTGATG AAGAATCACT GGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCAACA ACAGTGACTA AAACATTCAA GACAAGAAAA 
GCGTCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAGTCAAA AAGTAAGAAG 
AGGAATTCTA CTCAGCTGAA AAGCAGAGTT AAAAACATCA TTGTCACTTT TTTTACTCAA 
GGGTATGAAA GTGCCAGTAT GTCTAGCACA TGTGAACCTT GCAAAAGTAG GAACAGACAT 
TCAGCCCAGA CTGAAGAGCC TGTTCAAGCA AAAGTATTCA GCAGAAAGAA TCATGAGCAA 
CTGGAAAAAA TAATAAAATG TAATAGGTCT ACAGAAATAT CTTCAGCACA TGCTAGGAGA 
ATACTACAGC AGTCTAACAG AAATGCATGC AATGAAGCGC CAGAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG AGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC GTCCACATTT TCTTATTGAA CTCTTCCATG AGCTGCAGCT ACTAAACACA 
GACTACTTGA GACAGAGGGC TTTATATGCA TTGCAGGACA TAGTATCCAG ACATATTTCT 
GAGAGCCATG AAAAAGGAGA AAATGTAAAG TCAGTAAACT CTGGTACTTG GATAGCATCA 
AACTCAGAAC TTACTCCTAG TGAGAGCCTT GCTACTACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAAA GAGAAACCCA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGTGTC 
CTGTCTGTAT CATCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACCGTGATT 
CACTTAGATC AAGCATTAGC CAGAATGAGA GAATATGAGC GTATGAAGAC TGAGGCTGAA 
AGTAACTCAA ATATGAGATG CACCTGCAGG ATTATTGAGG ATGGAGATGG TGCTGGTGCA 
GGTACTACAG TTAATAATTT AGAAGAAACT CCCGTTATTG AAAATCGTAG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC CATCCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CAATTATGAA AGAAGTCATT CCTTTTTTGA AGGATTTTTC TCAGGAGCAC 
ATGGATGAAG TATGCTCCTC GCAGCTTCTA ACTTCAGTAA GGCGCATGGT TTTGACCCTT 
ACCCAGCAAA ATGATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTC AATGAATTGG CTTTCTTTAA GCTTATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCAACT 
GGAGTGATAC AATCTTGTGC CAAAGAGGCT AAAAGGATTC TTGAAGATCA TGGCTCACCT 
GCTGGAGAGA TTGATGATGA AGACAAAGAC AAGGATGAAA CTGAAACAGT TAAGCAGACT 
CAAACATCTG AGGTGTATGA TGGTCCCAAA AATGTAAGAT CTGATATTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGG ATGTCCAGTG TCTATTAATT TGTCTAAAGC TGAAACTCAG 
GCTTTAACTA ATTATGGAAG TGGAGAAGAT GAAAATGAGG ATGAAGAAAT GGAAGAATTT 
GAAGAAGGCC CTGTGGATGT CCAGACTTCC CTCCAGGCTA ACACTGAAGC TACTGAAGAA 
AATGAACATG ATGAACAGGT CCTACAACGT GACTTTAAAA AGACAGCAGA AAGCAAAAAT 
GTCCCATTGG AACGAGAAGC CACTAGTAAA AATGACCAAA ATAACTGTCC TGTGAAACCC 
TGTTACCTCA ATATCTTGGA AGATGAGCAA CCTTTAAATA GTGCTGCCCA TAAGGAGTCA 
CCTCCTACTG TTGATTCAAC TCAACAGCCT AACCCTTTGC CGTTACGTTT ACCTGAAATG 
GAACCCTTAG TGCCTAGAGT CAAAGAAGTT AAATCTGCTC AGGAAACTCC TGAAAGCTCT 
CTGGCTGGAA GTCCTGATAC TGAATCTCCA GTGTTAGTGA ATGACTATGA AGCAGAATCT 
GGTAATATAA GTCAAAAGTC TGATGAAGAA GATTTTGTAA AAGTTGAAGA TTTACCACTG 
AAACTGACAA TATATTCAGA GGCAGATCTA AGAAAGAAAA TGGTAGAAGA AGAACAGAAA 
AACCATTTAT CTGGTGAAAT ATGTGAAATG CAGACCGAAG AATTAGCTGG AAATTCTGAG 
ACACTAAAAG AACCTGAAAC GGTGGGAGCC CAGAGTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_017013479.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868968.1 Homo sapiens pericentriolar material 1 (PCM1), transcript variant X18, mRNA.

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CloneID	OHu59533
Clone ID Related Accession (Same CDS sequence)	XM_017013479.1
Accession Version	XM_017013479.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	pericentriolar material 1 protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCATTGT CACTTTTTTT 
ACTCAAGGGT ATGAAAGTGC CAGTATGTCT AGCACATGTG AACCTTGCAA AAGTAGGAAC 
AGACATTCAG CCCAGACTGA AGAGCCTGTT CAAGCAAAAG TATTCAGCAG AAAGAATCAT 
GAGCAACTGG AAAAAATAAT AAAATGTAAT AGGTCTACAG AAATATCTTC AGCACATGCT 
AGGAGAATAC TACAGCAGTC TAACAGAAAT GCATGCAATG AAGCGCCAGA AACTGGGAGT 
GATTTTTCCA TGTTTGAAGC TTTGCGAGAT ACTATTTATT CTGAAGTAGC TACATTAATT 
TCTCAAAATG AATCTCGTCC ACATTTTCTT ATTGAACTCT TCCATGAGCT GCAGCTACTA 
AACACAGACT ACTTGAGACA GAGGGCTTTA TATGCATTGC AGGACATAGT ATCCAGACAT 
ATTTCTGAGA GCCATGAAAA AGGAGAAAAT GTAAAGTCAG TAAACTCTGG TACTTGGATA 
GCATCAAACT CAGAACTTAC TCCTAGTGAG AGCCTTGCTA CTACTGATGA TGAAACTTTT 
GAGAAGAACT TTGAAAGAGA AACCCATAAA ATAAGTGAGC AAAATGATGC TGATAATGCT 
AGTGTCCTGT CTGTATCATC AAATTTTGAG CCTTTTGCAA CAGATGATCT AGGTAACACC 
GTGATTCACT TAGATCAAGC ATTAGCCAGA ATGAGAGAAT ATGAGCGTAT GAAGACTGAG 
GCTGAAAGTA ACTCAAATAT GAGATGCACC TGCAGGATTA TTGAGGATGG AGATGGTGCT 
GGTGCAGGTA CTACAGTTAA TAATTTAGAA GAAACTCCCG TTATTGAAAA TCGTAGTTCA 
CAACAACCTG TAAGTGAAGT TTCTACCATC CCATGTCCTA GAATTGATAC TCAGCAGCTG 
GACCGGCAAA TTAAAGCAAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA TTTTTCTCAG 
GAGCACATGG ATGAAGTATG CTCCTCGCAG CTTCTAACTT CAGTAAGGCG CATGGTTTTG 
ACCCTTACCC AGCAAAATGA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAACAACTT 
GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA AGACTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTGTTCAATG AATTGGCTTT CTTTAAGCTT 
ATGCAAGATT TGGATAATAA TAGTATAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCAACTGGAG TGATACAATC TTGTGCCAAA GAGGCTAAAA GGATTCTTGA AGATCATGGC 
TCACCTGCTG GAGAGATTGA TGATGAAGAC AAAGACAAGG ATGAAACTGA AACAGTTAAG 
CAGACTCAAA CATCTGAGGT GTATGATGGT CCCAAAAATG TAAGATCTGA TATTTCTGAT 
CAAGAGGAAG ATGAAGAAAG TGAAGGATGT CCAGTGTCTA TTAATTTGTC TAAAGCTGAA 
ACTCAGGCTT TAACTAATTA TGGAAGTGGA GAAGATGAAA ATGAGGATGA AGAAATGGAA 
GAATTTGAAG AAGGCCCTGT GGATGTCCAG ACTTCCCTCC AGGCTAACAC TGAAGCTACT 
GAAGAAAATG AACATGATGA ACAGGTCCTA CAACGTGACT TTAAAAAGAC AGCAGAAAGC 
AAAAATGTCC CATTGGAACG AGAAGCCACT AGTAAAAATG ACCAAAATAA CTGTCCTGTG 
AAACCCTGTT ACCTCAATAT CTTGGAAGAT GAGCAACCTT TAAATAGTGC TGCCCATAAG 
GAGTCACCTC CTACTGTTGA TTCAACTCAA CAGCCTAACC CTTTGCCGTT ACGTTTACCT 
GAAATGGAAC CCTTAGTGCC TAGAGTCAAA GAAGTTAAAT CTGCTCAGGA AACTCCTGAA 
AGCTCTCTGG CTGGAAGTCC TGATACTGAA TCTCCAGTGT TAGTGAATGA CTATGAAGCA 
GAATCTGGTA ATATAAGTCA AAAGTCTGAT GAAGAAGATT TTGTAAAAGT TGAAGATTTA 
CCACTGAAAC TGACAATATA TTCAGAGGCA GATCTAAGAA AGAAAATGGT AGAAGAAGAA 
CAGAAAAACC ATTTATCTGG TGAAATATGT GAAATGCAGA CCGAAGAATT AGCTGGAAAT 
TCTGAGACAC TAAAAGAACC TGAAACGGTG GGAGCCCAGA GTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868968.1
CDS	800..6967
Translation	MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLADCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIIVTFFTQGYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKDFSQEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Target ORF information:

RefSeq Version	XM_017013479.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pericentriolar material 1 (PCM1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013479.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC CTTTGAAGAT GGCATGAATG ATCAGGATTT ACCAAACTGG 
AGTAATGAGA ATGTTGATGA CAGGCTCAAC AATATGGATT GGGGTGCCCA ACAGAAGAAA 
GCAAATAGAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGAGTA 
ACCAATGATA TTTCTCCGGA GTCGTCACCA GGAGTTGGAA GGCGAAGAAC AAAGACTCCA 
CATACGTTCC CACACAGTAG ATACATGAGT CAGATGTCTG TCCCAGAGCA GGCAGAATTA 
GAGAAACTGA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT 
TCCCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAAAA CCGAAAGCCC 
TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCATC TACAAACCCC 
CCAAACAGAG AAACGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT 
AATGACCTCT TGCAAAACTG TCAGGTGTCT GAAGAAGATG GGAGGGGAGA ACCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAAATTCGCG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAGAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAG AAGTCATATA TGAAATTTCT TAAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAACAT 
GATTTATTAA AAAGAATGTT ACAACAGCAG GAGCAACTAA GAGCTCTACA GGGACGGCAG 
GCTGCACTTC TAGCTCTGCA ACATAAAGCA GAGCAAGCTA TTGCAGTGAT GGATGATTCT 
GTTGTTGCAG AAACTGCAGG TAGCTTATCT GGCGTCAGTA TCACATCTGA ACTAAATGAA 
GAATTGAATG ACTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAGCT 
GTTCCAGACA ATAGAAGACA GGCAGAAAGT CTTTCATTAA CTAGGGAGGT TTCCCAGAGC 
AGGAAACCAT CAGCTTCAGA ACGTTTACCT GATGAGAAAG TCGAACTTTT TAGCAAAATG 
AGAGTGCTAC AGGAAAAGAA ACAAAAAATG GACAAATTGC TTGGAGAACT TCATACACTT 
CGAGATCAGC ATCTTAACAA TTCATCATCC TCTCCACAAA GGAGTGTCGA TCAGAGAAGT 
ACTTCAGCTC CCTCTGCTTC TGTAGGCTTG GCACCGGTTG TCAATGGAGA ATCCAATAGC 
CTCACATCAT CTGTTCCTTA TCCTACTGCT TCTCTAGTAT CTCAGAATGA GAGTGAAAAC 
GAAGGCCACC TCAATCCATC TGAAAAACTC CAGAAGTTAA ATGAAGTTCG AAAGAGATTG 
AATGAGCTAA GAGAATTAGT TCATTATTAT GAACAAACGT CAGACATGAT GACAGATGCT 
GTGAATGAAA ACAGGAAAGA TGAAGAAACT GAAGAGTCAG AATATGATTC TGAGCATGAA 
AATTCCGAGC CTGTTACTAA CATTCGAAAT CCACAAGTAG CTTCCACTTG GAATGAAGTA 
AATAGTCATA GTAATGCACA GTGTGTTTCT AATAATAGAG ATGGGCGAAC AGTTAATTCT 
AATTGTGAAA TTAACAACAG ATCTGCTGCC AACATAAGGG CTCTAAACAT GCCTCCTTCT 
TTAGCAGATT GTCGATATAA TAGAGAAGGG GAACAGGAGA TTCATGTTGC ACAAGGTGAA 
GATGATGAGG AGGAGGAGGA AGAAGCAGAA GAGGAGGGAG TCAGTGGAGC TTCATTATCT 
AGTCACAGGA GCAGTCTGGT TGATGAGCAT CCAGAAGATG CTGAATTTGA ACAGAAGATC 
AACCGACTTA TGGCTGCAAA ACAGAAACTT AGACAGTTAC AAGATCTTGT TGCTATGGTA 
CAGGATGATG ATGCAGCTCA AGGAGTTATC TCTGCCAGTG CATCAAATTT GGATGATTTC 
TACCCAGCAG AAGAAGACAC CAAGCAAAAT TCAAATAACA CTAGAGGAAA TGCCAATAAA 
ACACAGAAAG ATACTGGAGT AAATGAAAAG GCAAGAGAGA AATTTTATGA GGCTAAACTA 
CAGCAGCAAC AGAGAGAGCT AAAACAATTG CAGGAAGAAA GAAAGAAACT GATTGACATT 
CAGGAGAAAA TTCAAGCATT GCAAACGGCA TGCCCTGACT TACAGCTGTC AGCTGCTAGT 
GTGGGTAACT GTCCCACCAA AAAATATATG CCAGCTGTTA CTTCAACCCC AACTGTTAAT 
CAACACGAGA CCAGTACAAG CAAATCTGTT TTTGAGCCTG AAGATTCTTC AATAGTAGAT 
AATGAGTTGT GGTCAGAAAT GAGAAGACAT GAAATGTTGA GGGAGGAGCT GCGACAGAGA 
AGAAAGCAGC TTGAAGCTCT GATGGCTGAA CATCAGAGGA GGCAAGGTCT AGCTGAAACT 
GCATCTCCAG TGGCTGTGTC ATTGAGAAGT GATGGATCTG AGAACCTATG TACTCCTCAG 
CAAAGTAGAA CAGAAAAAAC GATGGCAACT TGGGGAGGGT CTACCCAGTG TGCACTAGAT 
GAAGAAGGAG ATGAAGACGG TTACCTTTCT GAAGGAATTG TTCGGACAGA TGAAGAGGAG 
GAAGAAGAGC AAGATGCCAG TTCCAATGAT AACTTTTCTG TGTGTCCTTC TAACAGTGTG 
AATCATAACT CCTACAATGG AAAGGAAACT AAAAATAGGT GGAAGAACAA TTGCCCTTTT 
TCGGCAGATG AAAATTATCG TCCTTTAGCC AAGACAAGGC AACAGAATAT CAGCATGCAA 
CGGCAAGAAA ACCTTCGTTG GGTGTCAGAG CTCTCTTACG TAGAAGAGAA AGAACAATGG 
CAAGAACAAA TCAATCAGCT AAAGAAACAG CTTGATTTTA GTGTCAGTAT TTGTCAGACT 
TTGATGCAAG ACCAGCAGAC TCTATCTTGT CTGCTACAAA CTCTTCTCAC GGGTCCTTAC 
AGTGTTATGC CCAGCAATGT TGCATCTCCT CAAGTACACT TCATAATGCA CCAGTTGAAC 
CAGTGCTATA CTCAGCTAAC ATGGCAACAG AATAATGTTC AGAGGTTGAA ACAAATGCTA 
AATGAACTTA TGCGCCAGCA AAATCAGCAT CCAGAAAAAC CTGGAGGCAA GGAAAGAGGC 
AGTAGTGCAT CGCACCCTCC TTCTCCCAGT TTATTTTGTC CTTTCAGCTT TCCAACACAG 
CCTGTAAATC TCTTCAATAT ACCTGGATTT ACTAACTTTT CATCATTTGC ACCAGGTATG 
AATTTCAGCC CTTTATTTCC TTCTAATTTT GGAGATTTTT CTCAGAATAT CTCTACACCC 
AGTGAACAGC AGCAACCCTT AGCCCAGAAT TCTTCAGGAA AAACAGAATA TATGGCTTTT 
CCAAAACCTT TTGAAAGCAG TTCCTCTATT GGAGCAGAGA AACCAAGGAA TAAAAAACTG 
CCTGAAGAGG AGGTGGAAAG CAGTAGGACA CCATGGTTAT ATGAACAAGA AGGTGAAGTA 
GAGAAACCAT TTATCAAGAC TGGATTTTCA GTGTCTGTAG AAAAATCTAC AAGTAGTAAC 
CGCAAAAATC AATTAGATAC AAACGGAAGA AGACGCCAGT TTGATGAAGA ATCACTGGAA 
AGCTTTAGCA GTATGCCTGA TCCAGTAGAT CCAACAACAG TGACTAAAAC ATTCAAGACA 
AGAAAAGCGT CTGCACAGGC CAGCCTGGCA TCTAAAGATA AAACTCCCAA GTCAAAAAGT 
AAGAAGAGGA ATTCTACTCA GCTGAAAAGC AGAGTTAAAA ACATCATTGT CACTTTTTTT 
ACTCAAGGGT ATGAAAGTGC CAGTATGTCT AGCACATGTG AACCTTGCAA AAGTAGGAAC 
AGACATTCAG CCCAGACTGA AGAGCCTGTT CAAGCAAAAG TATTCAGCAG AAAGAATCAT 
GAGCAACTGG AAAAAATAAT AAAATGTAAT AGGTCTACAG AAATATCTTC AGCACATGCT 
AGGAGAATAC TACAGCAGTC TAACAGAAAT GCATGCAATG AAGCGCCAGA AACTGGGAGT 
GATTTTTCCA TGTTTGAAGC TTTGCGAGAT ACTATTTATT CTGAAGTAGC TACATTAATT 
TCTCAAAATG AATCTCGTCC ACATTTTCTT ATTGAACTCT TCCATGAGCT GCAGCTACTA 
AACACAGACT ACTTGAGACA GAGGGCTTTA TATGCATTGC AGGACATAGT ATCCAGACAT 
ATTTCTGAGA GCCATGAAAA AGGAGAAAAT GTAAAGTCAG TAAACTCTGG TACTTGGATA 
GCATCAAACT CAGAACTTAC TCCTAGTGAG AGCCTTGCTA CTACTGATGA TGAAACTTTT 
GAGAAGAACT TTGAAAGAGA AACCCATAAA ATAAGTGAGC AAAATGATGC TGATAATGCT 
AGTGTCCTGT CTGTATCATC AAATTTTGAG CCTTTTGCAA CAGATGATCT AGGTAACACC 
GTGATTCACT TAGATCAAGC ATTAGCCAGA ATGAGAGAAT ATGAGCGTAT GAAGACTGAG 
GCTGAAAGTA ACTCAAATAT GAGATGCACC TGCAGGATTA TTGAGGATGG AGATGGTGCT 
GGTGCAGGTA CTACAGTTAA TAATTTAGAA GAAACTCCCG TTATTGAAAA TCGTAGTTCA 
CAACAACCTG TAAGTGAAGT TTCTACCATC CCATGTCCTA GAATTGATAC TCAGCAGCTG 
GACCGGCAAA TTAAAGCAAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA TTTTTCTCAG 
GAGCACATGG ATGAAGTATG CTCCTCGCAG CTTCTAACTT CAGTAAGGCG CATGGTTTTG 
ACCCTTACCC AGCAAAATGA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAACAACTT 
GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA AGACTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTGTTCAATG AATTGGCTTT CTTTAAGCTT 
ATGCAAGATT TGGATAATAA TAGTATAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA 
GCAACTGGAG TGATACAATC TTGTGCCAAA GAGGCTAAAA GGATTCTTGA AGATCATGGC 
TCACCTGCTG GAGAGATTGA TGATGAAGAC AAAGACAAGG ATGAAACTGA AACAGTTAAG 
CAGACTCAAA CATCTGAGGT GTATGATGGT CCCAAAAATG TAAGATCTGA TATTTCTGAT 
CAAGAGGAAG ATGAAGAAAG TGAAGGATGT CCAGTGTCTA TTAATTTGTC TAAAGCTGAA 
ACTCAGGCTT TAACTAATTA TGGAAGTGGA GAAGATGAAA ATGAGGATGA AGAAATGGAA 
GAATTTGAAG AAGGCCCTGT GGATGTCCAG ACTTCCCTCC AGGCTAACAC TGAAGCTACT 
GAAGAAAATG AACATGATGA ACAGGTCCTA CAACGTGACT TTAAAAAGAC AGCAGAAAGC 
AAAAATGTCC CATTGGAACG AGAAGCCACT AGTAAAAATG ACCAAAATAA CTGTCCTGTG 
AAACCCTGTT ACCTCAATAT CTTGGAAGAT GAGCAACCTT TAAATAGTGC TGCCCATAAG 
GAGTCACCTC CTACTGTTGA TTCAACTCAA CAGCCTAACC CTTTGCCGTT ACGTTTACCT 
GAAATGGAAC CCTTAGTGCC TAGAGTCAAA GAAGTTAAAT CTGCTCAGGA AACTCCTGAA 
AGCTCTCTGG CTGGAAGTCC TGATACTGAA TCTCCAGTGT TAGTGAATGA CTATGAAGCA 
GAATCTGGTA ATATAAGTCA AAAGTCTGAT GAAGAAGATT TTGTAAAAGT TGAAGATTTA 
CCACTGAAAC TGACAATATA TTCAGAGGCA GATCTAAGAA AGAAAATGGT AGAAGAAGAA 
CAGAAAAACC ATTTATCTGG TGAAATATGT GAAATGCAGA CCGAAGAATT AGCTGGAAAT 
TCTGAGACAC TAAAAGAACC TGAAACGGTG GGAGCCCAGA GTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The X-linked deubiquitinase USP9X is an integral component of centrosome.
The Journal of biological chemistry292(31)12874-12884(2017 08)
Wang Q,Tang Y,Xu Y,Xu S,Jiang Y,Dong Q,Zhou Y,Ge W

Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy.
Current biology : CB27(14)2123-2136.e7(2017 Jul)
Joachim J,Razi M,Judith D,Wirth M,Calamita E,Encheva V,Dynlacht BD,Snijders AP,O'Reilly N,Jefferies HBJ,Tooze SA

The centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking.
Journal of cell science130(8)1450-1462(2017 04)
Conkar D,Culfa E,Odabasi E,Rauniyar N,Yates JR 3rd,Firat-Karalar EN

Tethering of an E3 ligase by PCM1 regulates the abundance of centrosomal KIAA0586/Talpid3 and promotes ciliogenesis.
eLife5(2016 05)
Wang L,Lee K,Malonis R,Sanchez I,Dynlacht BD

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PCM1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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