PCM1 cDNA ORF clone, Bos taurus(cattle)

The following PCM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa231453 XM_024986408.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231453 XM_024986407.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231454 XM_024986409.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231455 XM_024986410.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231456 XM_024986411.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231457 XM_024986412.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231458 XM_024986413.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231459 XM_024986414.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231460 XM_024986415.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231461 XM_024986416.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231462 XM_024986417.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231463 XM_024986418.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231464 XM_024986419.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231465 XM_024986420.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231466 XM_024986421.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231467 XM_024986422.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231468 XM_024986423.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231469 XM_024986424.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231470 XM_024986425.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231471 XM_024986426.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa231472 XM_024986427.1
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa50980 XM_010826077.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50980 XM_010826078.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50980 XM_010820273.3
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50980 XM_010820274.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50981 XM_010820275.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50981 XM_010826079.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50982 XM_010826080.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50982 XM_010820276.3
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50983 XM_010820277.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50983 XM_010826081.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50984 XM_010826082.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50984 XM_010820278.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50985 XM_010820279.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50985 XM_010826083.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50986 XM_010820280.3
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50986 XM_010826084.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50987 XM_010820281.3
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50987 XM_010826085.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50988 XM_010826086.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50988 XM_010820282.3
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50989 XM_010820283.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50989 XM_010826087.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50990 XM_010826088.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50990 XM_010820284.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50991 XM_010820285.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50991 XM_010826089.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50992 XM_010826090.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50992 XM_010820286.3
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50993 XM_010820287.3
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50993 XM_010826091.2
Latest version!
Bos taurus pericentriolar material 1 (PCM1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OBa50986 XM_010820280.2 Bos taurus pericentriolar material 1 (PCM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50988 XM_010820282.2 Bos taurus pericentriolar material 1 (PCM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50987 XM_010820281.2 Bos taurus pericentriolar material 1 (PCM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50980 XM_010820273.2 Bos taurus pericentriolar material 1 (PCM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50982 XM_010820276.2 Bos taurus pericentriolar material 1 (PCM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50992 XM_010820286.2 Bos taurus pericentriolar material 1 (PCM1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa50993 XM_010820287.2 Bos taurus pericentriolar material 1 (PCM1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa231453
    Clone ID Related Accession (Same CDS sequence) XM_024986407.1 , XM_024986408.1
    Accession Version XM_024986407.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA GCAAGTATGT
    GCTCAGTCTG CCATCTTAAG ATGGAAACCC TTACCTATGC CTTTTAAACT GTTGGTGGTG
    TCGTCAGGGA ATTATTGTAG CAAAGTAAGT TTCTTTTGTA ATAACTTTAT ATTAAAATTG
    AAAGTTTTAA ATGGAAGAGA ACAATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842175.1
    CDS359..7048
    Translation

    Target ORF information:

    RefSeq Version XM_024986407.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986407.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA GCAAGTATGT
    GCTCAGTCTG CCATCTTAAG ATGGAAACCC TTACCTATGC CTTTTAAACT GTTGGTGGTG
    TCGTCAGGGA ATTATTGTAG CAAAGTAAGT TTCTTTTGTA ATAACTTTAT ATTAAAATTG
    AAAGTTTTAA ATGGAAGAGA ACAATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231453
    Clone ID Related Accession (Same CDS sequence) XM_024986407.1 , XM_024986408.1
    Accession Version XM_024986408.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA GCAAGTATGT
    GCTCAGTCTG CCATCTTAAG ATGGAAACCC TTACCTATGC CTTTTAAACT GTTGGTGGTG
    TCGTCAGGGA ATTATTGTAG CAAAGTAAGT TTCTTTTGTA ATAACTTTAT ATTAAAATTG
    AAAGTTTTAA ATGGAAGAGA ACAATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842176.1
    CDS288..6977
    Translation

    Target ORF information:

    RefSeq Version XM_024986408.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986408.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA GCAAGTATGT
    GCTCAGTCTG CCATCTTAAG ATGGAAACCC TTACCTATGC CTTTTAAACT GTTGGTGGTG
    TCGTCAGGGA ATTATTGTAG CAAAGTAAGT TTCTTTTGTA ATAACTTTAT ATTAAAATTG
    AAAGTTTTAA ATGGAAGAGA ACAATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231454
    Clone ID Related Accession (Same CDS sequence) XM_024986409.1
    Accession Version XM_024986409.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6687bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GCACATGCTA GGAGAATACT ACAGCAGTCT AACAGAAATG CATGCAATGA AGCGCCAGAA
    ACCAGTAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT
    ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT
    CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA
    TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT
    GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT
    GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT
    GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGTGAC
    ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTACACGCA TATGAAGATG
    GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCGTTGAGGA TGACGATGGT
    GCTGCTGCCA CGACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT
    AGTTCCCAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG
    CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC
    ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT
    ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT
    ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT
    CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA
    GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT
    GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA
    CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGACTTTCT TTTGATGACA
    GGTGACCAGG ATAGCACTCC TGTGAAGCCA TGTTACCTCA ATATCGTGGA AAATGAGCAA
    CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCGTC CAGTCAACCT
    AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG
    AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA
    GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA
    GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA
    AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG
    CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAGCA AGTATGTGCT
    CAGTCTGCCA TCTTAAGATG GAAACCCTTA CCTATGCCTT TTAAACTGTT GGTGGTGTCG
    TCAGGGAATT ATTGTAGCAA AGTAAGTTTC TTTTGTAATA ACTTTATATT AAAATTGAAA
    GTTTTAAATG GAAGAGAACA ATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842177.1
    CDS359..7045
    Translation

    Target ORF information:

    RefSeq Version XM_024986409.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986409.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GCACATGCTA GGAGAATACT ACAGCAGTCT AACAGAAATG CATGCAATGA AGCGCCAGAA
    ACCAGTAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT
    ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT
    CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA
    TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT
    GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT
    GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT
    GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGTGAC
    ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTACACGCA TATGAAGATG
    GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCGTTGAGGA TGACGATGGT
    GCTGCTGCCA CGACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT
    AGTTCCCAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG
    CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC
    ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT
    ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT
    ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT
    CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA
    GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT
    GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA
    CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGACTTTCT TTTGATGACA
    GGTGACCAGG ATAGCACTCC TGTGAAGCCA TGTTACCTCA ATATCGTGGA AAATGAGCAA
    CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCGTC CAGTCAACCT
    AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG
    AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA
    GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA
    GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA
    AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG
    CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAGCA AGTATGTGCT
    CAGTCTGCCA TCTTAAGATG GAAACCCTTA CCTATGCCTT TTAAACTGTT GGTGGTGTCG
    TCAGGGAATT ATTGTAGCAA AGTAAGTTTC TTTTGTAATA ACTTTATATT AAAATTGAAA
    GTTTTAAATG GAAGAGAACA ATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231455
    Clone ID Related Accession (Same CDS sequence) XM_024986410.1
    Accession Version XM_024986410.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6672bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT
    GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC
    GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA
    GCTGGGAATT CTCAGACACT AAAAGAACCT GAGCAAGTAT GTGCTCAGTC TGCCATCTTA
    AGATGGAAAC CCTTACCTAT GCCTTTTAAA CTGTTGGTGG TGTCGTCAGG GAATTATTGT
    AGCAAAGTAA GTTTCTTTTG TAATAACTTT ATATTAAAAT TGAAAGTTTT AAATGGAAGA
    GAACAATATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842178.1
    CDS359..7030
    Translation

    Target ORF information:

    RefSeq Version XM_024986410.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986410.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT
    GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC
    GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA
    GCTGGGAATT CTCAGACACT AAAAGAACCT GAGCAAGTAT GTGCTCAGTC TGCCATCTTA
    AGATGGAAAC CCTTACCTAT GCCTTTTAAA CTGTTGGTGG TGTCGTCAGG GAATTATTGT
    AGCAAAGTAA GTTTCTTTTG TAATAACTTT ATATTAAAAT TGAAAGTTTT AAATGGAAGA
    GAACAATATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231456
    Clone ID Related Accession (Same CDS sequence) XM_024986411.1
    Accession Version XM_024986411.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGAAACCA GTAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA
    GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT
    GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC
    ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG
    AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT
    ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA
    AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG
    GGTGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA CACGCATATG
    AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCGT TGAGGATGAC
    GATGGTGCTG CTGCCACGAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA
    AATCATAGTT CCCAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT
    ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG
    GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG
    GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT
    GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA
    GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC
    ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA
    GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC
    AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT
    AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT
    ATTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAGTTTGTCC
    AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA
    GAGATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT
    GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA
    TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG
    ATGACAGGTG ACCAGGATAG CACTCCTGTG AAGCCATGTT ACCTCAATAT CGTGGAAAAT
    GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCGTCCAGT
    CAACCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA
    GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA
    TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT
    GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA
    GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT
    GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAGCAAGTA
    TGTGCTCAGT CTGCCATCTT AAGATGGAAA CCCTTACCTA TGCCTTTTAA ACTGTTGGTG
    GTGTCGTCAG GGAATTATTG TAGCAAAGTA AGTTTCTTTT GTAATAACTT TATATTAAAA
    TTGAAAGTTT TAAATGGAAG AGAACAATAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842179.1
    CDS359..6991
    Translation

    Target ORF information:

    RefSeq Version XM_024986411.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986411.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGAAACCA GTAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA
    GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT
    GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC
    ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG
    AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT
    ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA
    AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG
    GGTGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA CACGCATATG
    AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCGT TGAGGATGAC
    GATGGTGCTG CTGCCACGAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA
    AATCATAGTT CCCAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT
    ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG
    GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG
    GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT
    GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA
    GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC
    ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA
    GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC
    AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT
    AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT
    ATTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAGTTTGTCC
    AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA
    GAGATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT
    GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA
    TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG
    ATGACAGGTG ACCAGGATAG CACTCCTGTG AAGCCATGTT ACCTCAATAT CGTGGAAAAT
    GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCGTCCAGT
    CAACCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA
    GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA
    TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT
    GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA
    GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT
    GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAGCAAGTA
    TGTGCTCAGT CTGCCATCTT AAGATGGAAA CCCTTACCTA TGCCTTTTAA ACTGTTGGTG
    GTGTCGTCAG GGAATTATTG TAGCAAAGTA AGTTTCTTTT GTAATAACTT TATATTAAAA
    TTGAAAGTTT TAAATGGAAG AGAACAATAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231457
    Clone ID Related Accession (Same CDS sequence) XM_024986412.1
    Accession Version XM_024986412.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA GCAAGTATGT
    GCTCAGTCTG CCATCTTAAG ATGGAAACCC TTACCTATGC CTTTTAAACT GTTGGTGGTG
    TCGTCAGGGA ATTATTGTAG CAAAGTAAGT TTCTTTTGTA ATAACTTTAT ATTAAAATTG
    AAAGTTTTAA ATGGAAGAGA ACAATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842180.1
    CDS359..6988
    Translation

    Target ORF information:

    RefSeq Version XM_024986412.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986412.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA GCAAGTATGT
    GCTCAGTCTG CCATCTTAAG ATGGAAACCC TTACCTATGC CTTTTAAACT GTTGGTGGTG
    TCGTCAGGGA ATTATTGTAG CAAAGTAAGT TTCTTTTGTA ATAACTTTAT ATTAAAATTG
    AAAGTTTTAA ATGGAAGAGA ACAATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231458
    Clone ID Related Accession (Same CDS sequence) XM_024986413.1
    Accession Version XM_024986413.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGACTTTCT TTTGATGACA
    GGTGACCAGG ATAGCACTCC TGTGAAGCCA TGTTACCTCA ATATCGTGGA AAATGAGCAA
    CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCGTC CAGTCAACCT
    AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG
    AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA
    GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA
    GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA
    AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG
    CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAGCA AGTATGTGCT
    CAGTCTGCCA TCTTAAGATG GAAACCCTTA CCTATGCCTT TTAAACTGTT GGTGGTGTCG
    TCAGGGAATT ATTGTAGCAA AGTAAGTTTC TTTTGTAATA ACTTTATATT AAAATTGAAA
    GTTTTAAATG GAAGAGAACA ATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842181.1
    CDS359..6985
    Translation

    Target ORF information:

    RefSeq Version XM_024986413.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986413.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGACTTTCT TTTGATGACA
    GGTGACCAGG ATAGCACTCC TGTGAAGCCA TGTTACCTCA ATATCGTGGA AAATGAGCAA
    CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCGTC CAGTCAACCT
    AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG
    AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA
    GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA
    GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA
    AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG
    CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAGCA AGTATGTGCT
    CAGTCTGCCA TCTTAAGATG GAAACCCTTA CCTATGCCTT TTAAACTGTT GGTGGTGTCG
    TCAGGGAATT ATTGTAGCAA AGTAAGTTTC TTTTGTAATA ACTTTATATT AAAATTGAAA
    GTTTTAAATG GAAGAGAACA ATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231459
    Clone ID Related Accession (Same CDS sequence) XM_024986414.1
    Accession Version XM_024986414.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGCAGATCTA AGGAAGAAAA TGGCCGAAGA AGAACAGAAA
    AACCACTTAT CTGGTGAAAT ATGTGCAATG CAGACTGAAG AATTAGCTGG GAATTCTCAG
    ACACTAAAAG AACCTGAGCA AGTATGTGCT CAGTCTGCCA TCTTAAGATG GAAACCCTTA
    CCTATGCCTT TTAAACTGTT GGTGGTGTCG TCAGGGAATT ATTGTAGCAA AGTAAGTTTC
    TTTTGTAATA ACTTTATATT AAAATTGAAA GTTTTAAATG GAAGAGAACA ATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842182.1
    CDS359..6955
    Translation

    Target ORF information:

    RefSeq Version XM_024986414.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986414.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGCAGATCTA AGGAAGAAAA TGGCCGAAGA AGAACAGAAA
    AACCACTTAT CTGGTGAAAT ATGTGCAATG CAGACTGAAG AATTAGCTGG GAATTCTCAG
    ACACTAAAAG AACCTGAGCA AGTATGTGCT CAGTCTGCCA TCTTAAGATG GAAACCCTTA
    CCTATGCCTT TTAAACTGTT GGTGGTGTCG TCAGGGAATT ATTGTAGCAA AGTAAGTTTC
    TTTTGTAATA ACTTTATATT AAAATTGAAA GTTTTAAATG GAAGAGAACA ATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231460
    Clone ID Related Accession (Same CDS sequence) XM_024986415.1
    Accession Version XM_024986415.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6591bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC
    AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG
    TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT
    GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT
    GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT
    GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT
    ATGAAATTTC TCCAAAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA
    GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG
    TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG
    GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT
    CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA
    TGTAGCCGGA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATAGAA
    GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG
    CAGCTGAGAG CTCTACAGGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG
    CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT
    ACTGTACCAG GCAGACAGCC GGCTCGTTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA
    ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAATGAAGA ATTGAATGAT
    TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT
    AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCATCA
    GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA
    GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT
    TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA
    AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC
    AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT
    GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG
    AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA
    GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AGTCGGAATA TGATTCTGAG
    CATGAAAACT CTGAACCTGT TACTCATATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC
    GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCTAACA ATAGAGATGG GCGAGCAGTT
    AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT
    CCTCCTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA
    GGTCATGATG AGGAGGAGGA GGAGGAGGAA GCAGAAGATG AGGCCCTCAG TGGAGCTTCA
    CTGTCTAGTC ACAGCAGCAG CCTGATCATT GAGACTCCTG AAGATGCTGA ATTTGAACAT
    AAGATCCAGA AGCTTATGGC TGCAAAACTG AAACTTAGAC ACTTACAGAA CCTTGTAGCC
    ATGGTGCAGG ACGATGATGC AGCCAATCAA GGAGTTACTT CTGTGGAGGA TTTCTTCCCA
    GCAGAAGACA CCAAACAAAA TGCAAATAAC ACTAGAGGAA ATGCCAATAA AACACAAAAA
    GATGCTAGAG TAAATGAAAA GGCAAGAGAG AAATTTTATG AGGCTAAACT ACAGCAGCAA
    CAGAGAGAAC TCAAACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA
    ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC CTACAGCTGT CAGCTGCTAG TGTGGGTAAT
    TGTCCCACAA AAACACATAT GCCAGCTGTT ACTTCATCCC CAACCGTTAA TGAGAATGAA
    GCCAGTACAA ACAAATCTGG TTTTGAGCCT CATGATTCTT CAGTGGTAGA TAATGAGGTA
    TGGTCAGATA TGCGAAGACA TGAAATGTTA AGGGAGGAGC TACGACAGAG AAGAAAGCAG
    CTGGAAGCTC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TGGCTGAAAC TGCATCTCCA
    GTGGCTGTTT CACTGAGAAG TGATGGATCT GAGAACCTGT GTACACCTCA GCAAAGCAGA
    ACAGAAAAAA CAATGGCAAC TTGGGGAGGT TCTACCCAGT GTGCCCTAGA TGAAGAAGGG
    GATGAAGATG GTTACCTTTC TGAAGGAATT ATTCGGACAG ACGAAGAGGA GGAAGAGGAG
    GAGCAAGATG CCAGTTCCAA TGATAACTTT CATATGTATC CTCCAAACAG TGCAAATCGT
    AATTCTTACA ATGTAAAGGA AACTAAAAAT AGGTGGAAGA ACAATCGCCC TTTTTCAGCA
    GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAACAGA ATATCAGCAT GCAGCGTCAG
    GAAAACCTTC GCTGGGTGTC CGAACTCTCT TATGTAGAAG AGAAAGAACA ATGGCAAGAA
    CAAATTAATC AGCTAAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTAATG
    CAAGACCAGC AGACTCTGTC TTGTCTGCTA CAAACTCTTC TCACAAGTCC TTATAGTGTT
    ATGCCCAGCA GTGTTGCATC CCCGCAAGTG CACCTTATAA TGCACCAGTT GAACCAGTGC
    TATACCCAGC TAACGTGGCA GCAGAATAAT GTACAGAGGT TGAAACAAAT GCTCAGTGAA
    CTCATGCACC AACAAAATCA GCATCCAGAA AAACCTGCAA GCAAGGAAGG AGGCAGTGGT
    GCATCACACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA
    AATCTGTTTA ACCTACCGGG ATTTACTAAT TTTTCGTCAT TTGCACCAAG TATGAATTTC
    AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAAA ATATTTCTAC ACCCACTGAA
    CAGCAGCAAC CGTTAGCCCA GAATCCTTCA GGGAAAACAG AATACATGGC TTTTCCAAAA
    CCTTTTGAAA GTAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGAA
    GAGGAGGTGG AGAACAGTAG GACACCATGG TTTTATGACC AAGAAGGTGA GGTAGAAAAG
    CCATTTCTCA AGACTGGATT TGCAGTGTCT GTAGAGAAAA CAACAAATAG TAATCGCAAA
    AATCAACTAG ATACCAGTAG GAGAAGACGC CAGTTTGATG AAGTGTCCTT AGAAAGCTTT
    AACAGTATGC TTGACCCAGT AGATCCAACA ACAGTAACTA AAACATTCAA GTCAAGAAAA
    GCATCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAATCAAA GAGTAAGAAG
    AGGCATTCTG CTCAGCCGAA AAGCAGAGTG AAAAATATTG GGTATGAAAG TGCCAGTGTG
    TCTAGCACAT GTGAACCTTG CAAAAGTAGA AACAGACATT CAGCCCAGAC CGAAGAGCCT
    GTTCAAGCAA AAGTACTCAG CAGAAAGAAA CATGAGCAGC TGGAAAAAGT CATAAGATGT
    AGTAGGTCTA CAGAAATATC TTCAGCACAT GCTAGGAGAA TACTACAGCA GTCTAACAGA
    AATGCATGCA ATGAAGCGCC AGAAACCAGT AGTGATTTTT CCATGTTCGA AGCTTTGCGG
    GATACCATTT ATTCTGAAGT AGCTACGTTG ATTTCTCAAA ATGAATCTCG TCCACATTTT
    CTTATTGAAC TCTTCCATGA GCTTCAGCTA CTTAATACAG ACTATTTGAG ACAGAGGGCC
    TTATATGCAT TGCAGGACAT AGTATCCAGG CATATTTCTG AGAACCATGA AAAAGAAGGA
    GAAAATATAA AGTCCGTGAA TTCTGGTACT TGGATAGCAT CCAACTCAGA ACTTACTCCC
    AGTGAAAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA
    CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TATCTTCAAC TTGTGAGCCT
    TTTGCAACAG ATGTTCTGGG TGACACTGTG ATTCACTTAG ATCAGGCATT AGCCAGAATG
    AGAGACTACA CGCATATGAA GATGGAGGCT GAAAGTAGTC CAGACGTCAG GTGCACCTGC
    AGGATCGTTG AGGATGACGA TGGTGCTGCT GCCACGACCA CGATTAGTAA TAATTTAGAA
    GAAACTCCTG TTATTGAAAA TCATAGTTCC CAGCAACCCG TGAGTGAAGT TGCTACCGTC
    CCGTGTCCTA GAATTGATAC TCAGCAACTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA
    GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGTT CTTCTCAACT TCTAACCTCA
    GTAAGACGCA TGGTTTTGGT CCTTACTCAG CAAAACAATG AGAGCAAAGA GTTTGTAAAG
    TTCTTTCATA AGCAGCTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA
    AAACTGAAGG ATTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT ATTTAATGAA
    TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTGTAACTGT TAAACAGAGA
    TGCAAAAGGA AAATAGAAGC TGCTGGAGTG ATACAGTCTT ATGCAAAAGA GGCCAAAAGG
    ATTCTTGAAG GAGATCACAG TTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG
    GATGAGACGG AAACAGTTAA GCAGACTCAA ACGTCTGAGG TTTATGATGG TGACGGCCCC
    AAAAATGTGA GATCTGATAT TTCTGATCAA GAGGATGATG AAGAAAGTGA AGAATGTCCA
    GTGTCTATTA GTTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTACGG AAGTGGAGAA
    GATGAGAATG AAGATGAAGA GATAGAAGAA TTTGAAGAGG GACCTGTAGA TGTCCAGACG
    TCACTTCAGG CTAACACTGA AACTACAGAA GAAAATGAAC ACGATGATCA GGTTCCACAG
    CATGATTTTG AAAAATCATC AGAAAGCAAA AATGTCCCAT CAGAACAAGA ACCCACTACT
    AGTAAAGACT TTCTTTTGAT GACAGGTGAC CAGGATAGCA CTCCTGTGAA GCCATGTTAC
    CTCAATATCG TGGAAAATGA GCAACCTTTA AATAGCCCTG TCCAGAAGGA CACTCTCACT
    ACCATCGACT CGTCCAGTCA ACCTAACCCT TTGCCTTTAC CTTTAACTGA AATTGAAACC
    TTGGTGCCTA GAGTCAAAGA AGTGAAATCT GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT
    GGAAGTCCTG ATACTGAATC TCCAGTGTTA GTGAATGACT ATGAAGCAGA ATCTGGTAAC
    ATAAGTCAAA AATCTGATGA AGAAGACTTT GTGAAAGTTG AAGATTTACC ACTTAAACTG
    ACAATATATT CAGAGGCAGA TCTAAGGAAG AAAATGGCCG AAGAAGAACA GAAAAACCAC
    TTATCTGGTG AAATATGTGC AATGCAGACT GAAGAATTAG CTGGGAATTC TCAGACACTA
    AAAGAACCTG AGCAAGTATG TGCTCAGTCT GCCATCTTAA GATGGAAACC CTTACCTATG
    CCTTTTAAAC TGTTGGTGGT GTCGTCAGGG AATTATTGTA GCAAAGTAAG TTTCTTTTGT
    AATAACTTTA TATTAAAATT GAAAGTTTTA AATGGAAGAG AACAATATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842183.1
    CDS359..6949
    Translation

    Target ORF information:

    RefSeq Version XM_024986415.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986415.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC
    AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG
    TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT
    GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT
    GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT
    GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT
    ATGAAATTTC TCCAAAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA
    GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG
    TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG
    GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT
    CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA
    TGTAGCCGGA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATAGAA
    GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG
    CAGCTGAGAG CTCTACAGGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG
    CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT
    ACTGTACCAG GCAGACAGCC GGCTCGTTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA
    ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAATGAAGA ATTGAATGAT
    TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT
    AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCATCA
    GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA
    GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT
    TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA
    AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC
    AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT
    GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG
    AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA
    GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AGTCGGAATA TGATTCTGAG
    CATGAAAACT CTGAACCTGT TACTCATATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC
    GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCTAACA ATAGAGATGG GCGAGCAGTT
    AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT
    CCTCCTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA
    GGTCATGATG AGGAGGAGGA GGAGGAGGAA GCAGAAGATG AGGCCCTCAG TGGAGCTTCA
    CTGTCTAGTC ACAGCAGCAG CCTGATCATT GAGACTCCTG AAGATGCTGA ATTTGAACAT
    AAGATCCAGA AGCTTATGGC TGCAAAACTG AAACTTAGAC ACTTACAGAA CCTTGTAGCC
    ATGGTGCAGG ACGATGATGC AGCCAATCAA GGAGTTACTT CTGTGGAGGA TTTCTTCCCA
    GCAGAAGACA CCAAACAAAA TGCAAATAAC ACTAGAGGAA ATGCCAATAA AACACAAAAA
    GATGCTAGAG TAAATGAAAA GGCAAGAGAG AAATTTTATG AGGCTAAACT ACAGCAGCAA
    CAGAGAGAAC TCAAACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA
    ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC CTACAGCTGT CAGCTGCTAG TGTGGGTAAT
    TGTCCCACAA AAACACATAT GCCAGCTGTT ACTTCATCCC CAACCGTTAA TGAGAATGAA
    GCCAGTACAA ACAAATCTGG TTTTGAGCCT CATGATTCTT CAGTGGTAGA TAATGAGGTA
    TGGTCAGATA TGCGAAGACA TGAAATGTTA AGGGAGGAGC TACGACAGAG AAGAAAGCAG
    CTGGAAGCTC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TGGCTGAAAC TGCATCTCCA
    GTGGCTGTTT CACTGAGAAG TGATGGATCT GAGAACCTGT GTACACCTCA GCAAAGCAGA
    ACAGAAAAAA CAATGGCAAC TTGGGGAGGT TCTACCCAGT GTGCCCTAGA TGAAGAAGGG
    GATGAAGATG GTTACCTTTC TGAAGGAATT ATTCGGACAG ACGAAGAGGA GGAAGAGGAG
    GAGCAAGATG CCAGTTCCAA TGATAACTTT CATATGTATC CTCCAAACAG TGCAAATCGT
    AATTCTTACA ATGTAAAGGA AACTAAAAAT AGGTGGAAGA ACAATCGCCC TTTTTCAGCA
    GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAACAGA ATATCAGCAT GCAGCGTCAG
    GAAAACCTTC GCTGGGTGTC CGAACTCTCT TATGTAGAAG AGAAAGAACA ATGGCAAGAA
    CAAATTAATC AGCTAAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTAATG
    CAAGACCAGC AGACTCTGTC TTGTCTGCTA CAAACTCTTC TCACAAGTCC TTATAGTGTT
    ATGCCCAGCA GTGTTGCATC CCCGCAAGTG CACCTTATAA TGCACCAGTT GAACCAGTGC
    TATACCCAGC TAACGTGGCA GCAGAATAAT GTACAGAGGT TGAAACAAAT GCTCAGTGAA
    CTCATGCACC AACAAAATCA GCATCCAGAA AAACCTGCAA GCAAGGAAGG AGGCAGTGGT
    GCATCACACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA
    AATCTGTTTA ACCTACCGGG ATTTACTAAT TTTTCGTCAT TTGCACCAAG TATGAATTTC
    AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAAA ATATTTCTAC ACCCACTGAA
    CAGCAGCAAC CGTTAGCCCA GAATCCTTCA GGGAAAACAG AATACATGGC TTTTCCAAAA
    CCTTTTGAAA GTAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGAA
    GAGGAGGTGG AGAACAGTAG GACACCATGG TTTTATGACC AAGAAGGTGA GGTAGAAAAG
    CCATTTCTCA AGACTGGATT TGCAGTGTCT GTAGAGAAAA CAACAAATAG TAATCGCAAA
    AATCAACTAG ATACCAGTAG GAGAAGACGC CAGTTTGATG AAGTGTCCTT AGAAAGCTTT
    AACAGTATGC TTGACCCAGT AGATCCAACA ACAGTAACTA AAACATTCAA GTCAAGAAAA
    GCATCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAATCAAA GAGTAAGAAG
    AGGCATTCTG CTCAGCCGAA AAGCAGAGTG AAAAATATTG GGTATGAAAG TGCCAGTGTG
    TCTAGCACAT GTGAACCTTG CAAAAGTAGA AACAGACATT CAGCCCAGAC CGAAGAGCCT
    GTTCAAGCAA AAGTACTCAG CAGAAAGAAA CATGAGCAGC TGGAAAAAGT CATAAGATGT
    AGTAGGTCTA CAGAAATATC TTCAGCACAT GCTAGGAGAA TACTACAGCA GTCTAACAGA
    AATGCATGCA ATGAAGCGCC AGAAACCAGT AGTGATTTTT CCATGTTCGA AGCTTTGCGG
    GATACCATTT ATTCTGAAGT AGCTACGTTG ATTTCTCAAA ATGAATCTCG TCCACATTTT
    CTTATTGAAC TCTTCCATGA GCTTCAGCTA CTTAATACAG ACTATTTGAG ACAGAGGGCC
    TTATATGCAT TGCAGGACAT AGTATCCAGG CATATTTCTG AGAACCATGA AAAAGAAGGA
    GAAAATATAA AGTCCGTGAA TTCTGGTACT TGGATAGCAT CCAACTCAGA ACTTACTCCC
    AGTGAAAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA
    CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TATCTTCAAC TTGTGAGCCT
    TTTGCAACAG ATGTTCTGGG TGACACTGTG ATTCACTTAG ATCAGGCATT AGCCAGAATG
    AGAGACTACA CGCATATGAA GATGGAGGCT GAAAGTAGTC CAGACGTCAG GTGCACCTGC
    AGGATCGTTG AGGATGACGA TGGTGCTGCT GCCACGACCA CGATTAGTAA TAATTTAGAA
    GAAACTCCTG TTATTGAAAA TCATAGTTCC CAGCAACCCG TGAGTGAAGT TGCTACCGTC
    CCGTGTCCTA GAATTGATAC TCAGCAACTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA
    GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGTT CTTCTCAACT TCTAACCTCA
    GTAAGACGCA TGGTTTTGGT CCTTACTCAG CAAAACAATG AGAGCAAAGA GTTTGTAAAG
    TTCTTTCATA AGCAGCTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA
    AAACTGAAGG ATTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT ATTTAATGAA
    TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTGTAACTGT TAAACAGAGA
    TGCAAAAGGA AAATAGAAGC TGCTGGAGTG ATACAGTCTT ATGCAAAAGA GGCCAAAAGG
    ATTCTTGAAG GAGATCACAG TTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG
    GATGAGACGG AAACAGTTAA GCAGACTCAA ACGTCTGAGG TTTATGATGG TGACGGCCCC
    AAAAATGTGA GATCTGATAT TTCTGATCAA GAGGATGATG AAGAAAGTGA AGAATGTCCA
    GTGTCTATTA GTTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTACGG AAGTGGAGAA
    GATGAGAATG AAGATGAAGA GATAGAAGAA TTTGAAGAGG GACCTGTAGA TGTCCAGACG
    TCACTTCAGG CTAACACTGA AACTACAGAA GAAAATGAAC ACGATGATCA GGTTCCACAG
    CATGATTTTG AAAAATCATC AGAAAGCAAA AATGTCCCAT CAGAACAAGA ACCCACTACT
    AGTAAAGACT TTCTTTTGAT GACAGGTGAC CAGGATAGCA CTCCTGTGAA GCCATGTTAC
    CTCAATATCG TGGAAAATGA GCAACCTTTA AATAGCCCTG TCCAGAAGGA CACTCTCACT
    ACCATCGACT CGTCCAGTCA ACCTAACCCT TTGCCTTTAC CTTTAACTGA AATTGAAACC
    TTGGTGCCTA GAGTCAAAGA AGTGAAATCT GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT
    GGAAGTCCTG ATACTGAATC TCCAGTGTTA GTGAATGACT ATGAAGCAGA ATCTGGTAAC
    ATAAGTCAAA AATCTGATGA AGAAGACTTT GTGAAAGTTG AAGATTTACC ACTTAAACTG
    ACAATATATT CAGAGGCAGA TCTAAGGAAG AAAATGGCCG AAGAAGAACA GAAAAACCAC
    TTATCTGGTG AAATATGTGC AATGCAGACT GAAGAATTAG CTGGGAATTC TCAGACACTA
    AAAGAACCTG AGCAAGTATG TGCTCAGTCT GCCATCTTAA GATGGAAACC CTTACCTATG
    CCTTTTAAAC TGTTGGTGGT GTCGTCAGGG AATTATTGTA GCAAAGTAAG TTTCTTTTGT
    AATAACTTTA TATTAAAATT GAAAGTTTTA AATGGAAGAG AACAATATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231461
    Clone ID Related Accession (Same CDS sequence) XM_024986416.1
    Accession Version XM_024986416.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GCACATGCTA GGAGAATACT ACAGCAGTCT AACAGAAATG CATGCAATGA AGCGCCAGAA
    ACCAGTAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT
    ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT
    CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA
    TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT
    GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT
    GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT
    GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGTGAC
    ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTACACGCA TATGAAGATG
    GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCGTTGAGGA TGACGATGGT
    GCTGCTGCCA CGACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT
    AGTTCCCAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG
    CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC
    ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT
    ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT
    ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT
    CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA
    GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT
    GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA
    CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGGTGACCA GGATAGCACT
    CCTGTGAAGC CATGTTACCT CAATATCGTG GAAAATGAGC AACCTTTAAA TAGCCCTGTC
    CAGAAGGACA CTCTCACTAC CATCGACTCG TCCAGTCAAC CTAACCCTTT GCCTTTACCT
    TTAACTGAAA TTGAAACCTT GGTGCCTAGA GTCAAAGAAG TGAAATCTGC TCAGGAGACT
    CCTGAAAGTT CTTTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT
    GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA
    GATTTACCAC TTAAACTGAC AATATATTCA GAGGCAGATC TAAGGAAGAA AATGGCCGAA
    GAAGAACAGA AAAACCACTT ATCTGGTGAA ATATGTGCAA TGCAGACTGA AGAATTAGCT
    GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGTAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842184.1
    CDS359..6892
    Translation

    Target ORF information:

    RefSeq Version XM_024986416.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986416.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GCACATGCTA GGAGAATACT ACAGCAGTCT AACAGAAATG CATGCAATGA AGCGCCAGAA
    ACCAGTAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT
    ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT
    CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA
    TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT
    GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT
    GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT
    GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGTGAC
    ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTACACGCA TATGAAGATG
    GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCGTTGAGGA TGACGATGGT
    GCTGCTGCCA CGACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT
    AGTTCCCAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG
    CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC
    ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT
    ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT
    ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT
    CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA
    GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT
    GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA
    CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGGTGACCA GGATAGCACT
    CCTGTGAAGC CATGTTACCT CAATATCGTG GAAAATGAGC AACCTTTAAA TAGCCCTGTC
    CAGAAGGACA CTCTCACTAC CATCGACTCG TCCAGTCAAC CTAACCCTTT GCCTTTACCT
    TTAACTGAAA TTGAAACCTT GGTGCCTAGA GTCAAAGAAG TGAAATCTGC TCAGGAGACT
    CCTGAAAGTT CTTTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT
    GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA
    GATTTACCAC TTAAACTGAC AATATATTCA GAGGCAGATC TAAGGAAGAA AATGGCCGAA
    GAAGAACAGA AAAACCACTT ATCTGGTGAA ATATGTGCAA TGCAGACTGA AGAATTAGCT
    GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGTAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231462
    Clone ID Related Accession (Same CDS sequence) XM_024986417.1
    Accession Version XM_024986417.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG CAGATTGTCG ATACAATAGA
    GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGATG AGGAGGAGGA GGAGGAGGAA
    GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT
    GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG
    AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA
    GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC
    ACTAGAGGAA ATGCCAATAA AACACAAAAA GATGCTAGAG TAAATGAAAA GGCAAGAGAG
    AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA
    AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC
    CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT
    ACTTCATCCC CAACCGTTAA TGAGAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT
    CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA
    AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG
    AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT
    GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT
    TCTACCCAGT GTGCCCTAGA TGAAGAAGGG GATGAAGATG GTTACCTTTC TGAAGGAATT
    ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT
    CATATGTATC CTCCAAACAG TGCAAATCGT AATTCTTACA ATGTAAAGGA AACTAAAAAT
    AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA
    AGACAACAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT
    TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT
    TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA
    CAAACTCTTC TCACAAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG
    CACCTTATAA TGCACCAGTT GAACCAGTGC TATACCCAGC TAACGTGGCA GCAGAATAAT
    GTACAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCACC AACAAAATCA GCATCCAGAA
    AAACCTGCAA GCAAGGAAGG AGGCAGTGGT GCATCACACC CTCCTTCTCC CAGTTTATTT
    TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG ATTTACTAAT
    TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT
    TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA
    GGGAAAACAG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCA
    GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG
    TTTTATGACC AAGAAGGTGA GGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT
    GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC
    CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA
    ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA
    GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTG CTCAGCCGAA AAGCAGAGTG
    AAAAATATTG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA
    AACAGACATT CAGCCCAGAC CGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAA
    CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGCACAT
    GCTAGGAGAA TACTACAGCA GTCTAACAGA AATGCATGCA ATGAAGCGCC AGAAACCAGT
    AGTGATTTTT CCATGTTCGA AGCTTTGCGG GATACCATTT ATTCTGAAGT AGCTACGTTG
    ATTTCTCAAA ATGAATCTCG TCCACATTTT CTTATTGAAC TCTTCCATGA GCTTCAGCTA
    CTTAATACAG ACTATTTGAG ACAGAGGGCC TTATATGCAT TGCAGGACAT AGTATCCAGG
    CATATTTCTG AGAACCATGA AAAAGAAGGA GAAAATATAA AGTCCGTGAA TTCTGGTACT
    TGGATAGCAT CCAACTCAGA ACTTACTCCC AGTGAAAGCC TTGCTACTAC TGATGATGAA
    ACTTTTGAGA AGAACTTTGA GAGAGAAACA CATAAAATAA GTGAGCAAAA TGATGCTGAT
    AATGCTAGTG TATCTTCAAC TTGTGAGCCT TTTGCAACAG ATGTTCTGGG TGACACTGTG
    ATTCACTTAG ATCAGGCATT AGCCAGAATG AGAGACTACA CGCATATGAA GATGGAGGCT
    GAAAGTAGTC CAGACGTCAG GTGCACCTGC AGGATCGTTG AGGATGACGA TGGTGCTGCT
    GCCACGACCA CGATTAGTAA TAATTTAGAA GAAACTCCTG TTATTGAAAA TCATAGTTCC
    CAGCAACCCG TGAGTGAAGT TGCTACCGTC CCGTGTCCTA GAATTGATAC TCAGCAACTG
    GACCGGCAAA TTAAAGCAAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT
    GAAGTATGTT CTTCTCAACT TCTAACCTCA GTAAGACGCA TGGTTTTGGT CCTTACTCAG
    CAAAACAATG AGAGCAAAGA GTTTGTAAAG TTCTTTCATA AGCAGCTTGG AAGTATATTA
    CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAGG ATTGTGGAGA AGATCTTCTT
    GTAGAGATAT CTGAAGTGTT ATTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG
    GATAATAATA GTGTAACTGT TAAACAGAGA TGCAAAAGGA AAATAGAAGC TGCTGGAGTG
    ATACAGTCTT ATGCAAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCACAG TTCACCTGCT
    GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAGACGG AAACAGTTAA GCAGACTCAA
    ACGTCTGAGG TTTATGATGG TGACGGCCCC AAAAATGTGA GATCTGATAT TTCTGATCAA
    GAGGATGATG AAGAAAGTGA AGAATGTCCA GTGTCTATTA GTTTGTCCAA AGCTGAAACT
    CAGGCTTTGA CTAATTACGG AAGTGGAGAA GATGAGAATG AAGATGAAGA GATAGAAGAA
    TTTGAAGAGG GACCTGTAGA TGTCCAGACG TCACTTCAGG CTAACACTGA AACTACAGAA
    GAAAATGAAC ACGATGATCA GGTTCCACAG CATGATTTTG AAAAATCATC AGAAAGCAAA
    AATGTCCCAT CAGAACAAGA ACCCACTACT AGTAAAGACT TTCTTTTGAT GACAGGTGAC
    CAGGATAGCA CTCCTGTGAA GCCATGTTAC CTCAATATCG TGGAAAATGA GCAACCTTTA
    AATAGCCCTG TCCAGAAGGA CACTCTCACT ACCATCGACT CGTCCAGTCA ACCTAACCCT
    TTGCCTTTAC CTTTAACTGA AATTGAAACC TTGGTGCCTA GAGTCAAAGA AGTGAAATCT
    GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA
    GTGAATGACT ATGAAGCAGA ATCTGGTAAC ATAAGTCAAA AATCTGATGA AGAAGACTTT
    GTGAAAGTTG AAGATTTACC ACTTAAACTG ACAATATATT CAGAGGCAGA TCTAAGGAAG
    AAAATGGCCG AAGAAGAACA GAAAAACCAC TTATCTGGTG AAATATGTGC AATGCAGACT
    GAAGAATTAG CTGGGAATTC TCAGACACTA AAAGAACCTG AGCAAGTATG TGCTCAGTCT
    GCCATCTTAA GATGGAAACC CTTACCTATG CCTTTTAAAC TGTTGGTGGT GTCGTCAGGG
    AATTATTGTA GCAAAGTAAG TTTCTTTTGT AATAACTTTA TATTAAAATT GAAAGTTTTA
    AATGGAAGAG AACAATATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842185.1
    CDS359..6859
    Translation

    Target ORF information:

    RefSeq Version XM_024986417.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986417.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG CAGATTGTCG ATACAATAGA
    GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGATG AGGAGGAGGA GGAGGAGGAA
    GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT
    GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG
    AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA
    GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC
    ACTAGAGGAA ATGCCAATAA AACACAAAAA GATGCTAGAG TAAATGAAAA GGCAAGAGAG
    AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA
    AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC
    CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT
    ACTTCATCCC CAACCGTTAA TGAGAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT
    CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA
    AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG
    AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT
    GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT
    TCTACCCAGT GTGCCCTAGA TGAAGAAGGG GATGAAGATG GTTACCTTTC TGAAGGAATT
    ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT
    CATATGTATC CTCCAAACAG TGCAAATCGT AATTCTTACA ATGTAAAGGA AACTAAAAAT
    AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA
    AGACAACAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT
    TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT
    TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA
    CAAACTCTTC TCACAAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG
    CACCTTATAA TGCACCAGTT GAACCAGTGC TATACCCAGC TAACGTGGCA GCAGAATAAT
    GTACAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCACC AACAAAATCA GCATCCAGAA
    AAACCTGCAA GCAAGGAAGG AGGCAGTGGT GCATCACACC CTCCTTCTCC CAGTTTATTT
    TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG ATTTACTAAT
    TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT
    TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA
    GGGAAAACAG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCA
    GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG
    TTTTATGACC AAGAAGGTGA GGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT
    GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC
    CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA
    ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA
    GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTG CTCAGCCGAA AAGCAGAGTG
    AAAAATATTG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA
    AACAGACATT CAGCCCAGAC CGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAA
    CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGCACAT
    GCTAGGAGAA TACTACAGCA GTCTAACAGA AATGCATGCA ATGAAGCGCC AGAAACCAGT
    AGTGATTTTT CCATGTTCGA AGCTTTGCGG GATACCATTT ATTCTGAAGT AGCTACGTTG
    ATTTCTCAAA ATGAATCTCG TCCACATTTT CTTATTGAAC TCTTCCATGA GCTTCAGCTA
    CTTAATACAG ACTATTTGAG ACAGAGGGCC TTATATGCAT TGCAGGACAT AGTATCCAGG
    CATATTTCTG AGAACCATGA AAAAGAAGGA GAAAATATAA AGTCCGTGAA TTCTGGTACT
    TGGATAGCAT CCAACTCAGA ACTTACTCCC AGTGAAAGCC TTGCTACTAC TGATGATGAA
    ACTTTTGAGA AGAACTTTGA GAGAGAAACA CATAAAATAA GTGAGCAAAA TGATGCTGAT
    AATGCTAGTG TATCTTCAAC TTGTGAGCCT TTTGCAACAG ATGTTCTGGG TGACACTGTG
    ATTCACTTAG ATCAGGCATT AGCCAGAATG AGAGACTACA CGCATATGAA GATGGAGGCT
    GAAAGTAGTC CAGACGTCAG GTGCACCTGC AGGATCGTTG AGGATGACGA TGGTGCTGCT
    GCCACGACCA CGATTAGTAA TAATTTAGAA GAAACTCCTG TTATTGAAAA TCATAGTTCC
    CAGCAACCCG TGAGTGAAGT TGCTACCGTC CCGTGTCCTA GAATTGATAC TCAGCAACTG
    GACCGGCAAA TTAAAGCAAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT
    GAAGTATGTT CTTCTCAACT TCTAACCTCA GTAAGACGCA TGGTTTTGGT CCTTACTCAG
    CAAAACAATG AGAGCAAAGA GTTTGTAAAG TTCTTTCATA AGCAGCTTGG AAGTATATTA
    CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAGG ATTGTGGAGA AGATCTTCTT
    GTAGAGATAT CTGAAGTGTT ATTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG
    GATAATAATA GTGTAACTGT TAAACAGAGA TGCAAAAGGA AAATAGAAGC TGCTGGAGTG
    ATACAGTCTT ATGCAAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCACAG TTCACCTGCT
    GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAGACGG AAACAGTTAA GCAGACTCAA
    ACGTCTGAGG TTTATGATGG TGACGGCCCC AAAAATGTGA GATCTGATAT TTCTGATCAA
    GAGGATGATG AAGAAAGTGA AGAATGTCCA GTGTCTATTA GTTTGTCCAA AGCTGAAACT
    CAGGCTTTGA CTAATTACGG AAGTGGAGAA GATGAGAATG AAGATGAAGA GATAGAAGAA
    TTTGAAGAGG GACCTGTAGA TGTCCAGACG TCACTTCAGG CTAACACTGA AACTACAGAA
    GAAAATGAAC ACGATGATCA GGTTCCACAG CATGATTTTG AAAAATCATC AGAAAGCAAA
    AATGTCCCAT CAGAACAAGA ACCCACTACT AGTAAAGACT TTCTTTTGAT GACAGGTGAC
    CAGGATAGCA CTCCTGTGAA GCCATGTTAC CTCAATATCG TGGAAAATGA GCAACCTTTA
    AATAGCCCTG TCCAGAAGGA CACTCTCACT ACCATCGACT CGTCCAGTCA ACCTAACCCT
    TTGCCTTTAC CTTTAACTGA AATTGAAACC TTGGTGCCTA GAGTCAAAGA AGTGAAATCT
    GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA
    GTGAATGACT ATGAAGCAGA ATCTGGTAAC ATAAGTCAAA AATCTGATGA AGAAGACTTT
    GTGAAAGTTG AAGATTTACC ACTTAAACTG ACAATATATT CAGAGGCAGA TCTAAGGAAG
    AAAATGGCCG AAGAAGAACA GAAAAACCAC TTATCTGGTG AAATATGTGC AATGCAGACT
    GAAGAATTAG CTGGGAATTC TCAGACACTA AAAGAACCTG AGCAAGTATG TGCTCAGTCT
    GCCATCTTAA GATGGAAACC CTTACCTATG CCTTTTAAAC TGTTGGTGGT GTCGTCAGGG
    AATTATTGTA GCAAAGTAAG TTTCTTTTGT AATAACTTTA TATTAAAATT GAAAGTTTTA
    AATGGAAGAG AACAATATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231463
    Clone ID Related Accession (Same CDS sequence) XM_024986418.1
    Accession Version XM_024986418.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG ATTGTCGATA CAATAGAGAA
    GGAGAACAGG AGATGCCTGT TGCACAAGGT CATGATGAGG AGGAGGAGGA GGAGGAAGCA
    GAAGATGAGG CCCTCAGTGG AGCTTCACTG TCTAGTCACA GCAGCAGCCT GATCATTGAG
    ACTCCTGAAG ATGCTGAATT TGAACATAAG ATCCAGAAGC TTATGGCTGC AAAACTGAAA
    CTTAGACACT TACAGAACCT TGTAGCCATG GTGCAGGACG ATGATGCAGC CAATCAAGGA
    GTTACTTCTG TGGAGGATTT CTTCCCAGCA GAAGACACCA AACAAAATGC AAATAACACT
    AGAGGAAATG CCAATAAAAC ACAAAAAGAT GCTAGAGTAA ATGAAAAGGC AAGAGAGAAA
    TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAACTCA AACAATTGCA GGAAGAAAGA
    AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGAATTGC AAAAGGCATG TCCTAACCTA
    CAGCTGTCAG CTGCTAGTGT GGGTAATTGT CCCACAAAAA CACATATGCC AGCTGTTACT
    TCATCCCCAA CCGTTAATGA GAATGAAGCC AGTACAAACA AATCTGGTTT TGAGCCTCAT
    GATTCTTCAG TGGTAGATAA TGAGGTATGG TCAGATATGC GAAGACATGA AATGTTAAGG
    GAGGAGCTAC GACAGAGAAG AAAGCAGCTG GAAGCTCTGA TGGCTGAACA TCAGAGGAGG
    CAAGGTCTGG CTGAAACTGC ATCTCCAGTG GCTGTTTCAC TGAGAAGTGA TGGATCTGAG
    AACCTGTGTA CACCTCAGCA AAGCAGAACA GAAAAAACAA TGGCAACTTG GGGAGGTTCT
    ACCCAGTGTG CCCTAGATGA AGAAGGGGAT GAAGATGGTT ACCTTTCTGA AGGAATTATT
    CGGACAGACG AAGAGGAGGA AGAGGAGGAG CAAGATGCCA GTTCCAATGA TAACTTTCAT
    ATGTATCCTC CAAACAGTGC AAATCGTAAT TCTTACAATG TAAAGGAAAC TAAAAATAGG
    TGGAAGAACA ATCGCCCTTT TTCAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA
    CAACAGAATA TCAGCATGCA GCGTCAGGAA AACCTTCGCT GGGTGTCCGA ACTCTCTTAT
    GTAGAAGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TAAAGAAACA ACTTGATTTC
    AGTGTCAATA TTTGTCAGAC TTTAATGCAA GACCAGCAGA CTCTGTCTTG TCTGCTACAA
    ACTCTTCTCA CAAGTCCTTA TAGTGTTATG CCCAGCAGTG TTGCATCCCC GCAAGTGCAC
    CTTATAATGC ACCAGTTGAA CCAGTGCTAT ACCCAGCTAA CGTGGCAGCA GAATAATGTA
    CAGAGGTTGA AACAAATGCT CAGTGAACTC ATGCACCAAC AAAATCAGCA TCCAGAAAAA
    CCTGCAAGCA AGGAAGGAGG CAGTGGTGCA TCACACCCTC CTTCTCCCAG TTTATTTTGT
    CCTTTCAACT TTCCAACTCA GCCTGTAAAT CTGTTTAACC TACCGGGATT TACTAATTTT
    TCGTCATTTG CACCAAGTAT GAATTTCAGC CCTTTATTTC CTTCTAATTT TGGAGATTTT
    TCTCAAAATA TTTCTACACC CACTGAACAG CAGCAACCGT TAGCCCAGAA TCCTTCAGGG
    AAAACAGAAT ACATGGCTTT TCCAAAACCT TTTGAAAGTA GTTCTTCTAT TGGAGCAGAA
    AAACAAAGGA ATCAAAAACA GCCTGAAGAG GAGGTGGAGA ACAGTAGGAC ACCATGGTTT
    TATGACCAAG AAGGTGAGGT AGAAAAGCCA TTTCTCAAGA CTGGATTTGC AGTGTCTGTA
    GAGAAAACAA CAAATAGTAA TCGCAAAAAT CAACTAGATA CCAGTAGGAG AAGACGCCAG
    TTTGATGAAG TGTCCTTAGA AAGCTTTAAC AGTATGCTTG ACCCAGTAGA TCCAACAACA
    GTAACTAAAA CATTCAAGTC AAGAAAAGCA TCTGCACAGG CCAGCCTGGC ATCTAAAGAT
    AAAACTCCCA AATCAAAGAG TAAGAAGAGG CATTCTGCTC AGCCGAAAAG CAGAGTGAAA
    AATATTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGAAAC
    AGACATTCAG CCCAGACCGA AGAGCCTGTT CAAGCAAAAG TACTCAGCAG AAAGAAACAT
    GAGCAGCTGG AAAAAGTCAT AAGATGTAGT AGGTCTACAG AAATATCTTC AGCACATGCT
    AGGAGAATAC TACAGCAGTC TAACAGAAAT GCATGCAATG AAGCGCCAGA AACCAGTAGT
    GATTTTTCCA TGTTCGAAGC TTTGCGGGAT ACCATTTATT CTGAAGTAGC TACGTTGATT
    TCTCAAAATG AATCTCGTCC ACATTTTCTT ATTGAACTCT TCCATGAGCT TCAGCTACTT
    AATACAGACT ATTTGAGACA GAGGGCCTTA TATGCATTGC AGGACATAGT ATCCAGGCAT
    ATTTCTGAGA ACCATGAAAA AGAAGGAGAA AATATAAAGT CCGTGAATTC TGGTACTTGG
    ATAGCATCCA ACTCAGAACT TACTCCCAGT GAAAGCCTTG CTACTACTGA TGATGAAACT
    TTTGAGAAGA ACTTTGAGAG AGAAACACAT AAAATAAGTG AGCAAAATGA TGCTGATAAT
    GCTAGTGTAT CTTCAACTTG TGAGCCTTTT GCAACAGATG TTCTGGGTGA CACTGTGATT
    CACTTAGATC AGGCATTAGC CAGAATGAGA GACTACACGC ATATGAAGAT GGAGGCTGAA
    AGTAGTCCAG ACGTCAGGTG CACCTGCAGG ATCGTTGAGG ATGACGATGG TGCTGCTGCC
    ACGACCACGA TTAGTAATAA TTTAGAAGAA ACTCCTGTTA TTGAAAATCA TAGTTCCCAG
    CAACCCGTGA GTGAAGTTGC TACCGTCCCG TGTCCTAGAA TTGATACTCA GCAACTGGAC
    CGGCAAATTA AAGCAATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGAGCA CATGGATGAA
    GTATGTTCTT CTCAACTTCT AACCTCAGTA AGACGCATGG TTTTGGTCCT TACTCAGCAA
    AACAATGAGA GCAAAGAGTT TGTAAAGTTC TTTCATAAGC AGCTTGGAAG TATATTACAG
    GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAGGATT GTGGAGAAGA TCTTCTTGTA
    GAGATATCTG AAGTGTTATT TAATGAATTG GCTTTCTTTA AGCTCATGCA AGATTTGGAT
    AATAATAGTG TAACTGTTAA ACAGAGATGC AAAAGGAAAA TAGAAGCTGC TGGAGTGATA
    CAGTCTTATG CAAAAGAGGC CAAAAGGATT CTTGAAGGAG ATCACAGTTC ACCTGCTGGA
    GAAATTGATG ATGAAGACAA AGACAAGGAT GAGACGGAAA CAGTTAAGCA GACTCAAACG
    TCTGAGGTTT ATGATGGTGA CGGCCCCAAA AATGTGAGAT CTGATATTTC TGATCAAGAG
    GATGATGAAG AAAGTGAAGA ATGTCCAGTG TCTATTAGTT TGTCCAAAGC TGAAACTCAG
    GCTTTGACTA ATTACGGAAG TGGAGAAGAT GAGAATGAAG ATGAAGAGAT AGAAGAATTT
    GAAGAGGGAC CTGTAGATGT CCAGACGTCA CTTCAGGCTA ACACTGAAAC TACAGAAGAA
    AATGAACACG ATGATCAGGT TCCACAGCAT GATTTTGAAA AATCATCAGA AAGCAAAAAT
    GTCCCATCAG AACAAGAACC CACTACTAGT AAAGACTTTC TTTTGATGAC AGGTGACCAG
    GATAGCACTC CTGTGAAGCC ATGTTACCTC AATATCGTGG AAAATGAGCA ACCTTTAAAT
    AGCCCTGTCC AGAAGGACAC TCTCACTACC ATCGACTCGT CCAGTCAACC TAACCCTTTG
    CCTTTACCTT TAACTGAAAT TGAAACCTTG GTGCCTAGAG TCAAAGAAGT GAAATCTGCT
    CAGGAGACTC CTGAAAGTTC TTTGGCTGGA AGTCCTGATA CTGAATCTCC AGTGTTAGTG
    AATGACTATG AAGCAGAATC TGGTAACATA AGTCAAAAAT CTGATGAAGA AGACTTTGTG
    AAAGTTGAAG ATTTACCACT TAAACTGACA ATATATTCAG AGGCAGATCT AAGGAAGAAA
    ATGGCCGAAG AAGAACAGAA AAACCACTTA TCTGGTGAAA TATGTGCAAT GCAGACTGAA
    GAATTAGCTG GGAATTCTCA GACACTAAAA GAACCTGAGC AAGTATGTGC TCAGTCTGCC
    ATCTTAAGAT GGAAACCCTT ACCTATGCCT TTTAAACTGT TGGTGGTGTC GTCAGGGAAT
    TATTGTAGCA AAGTAAGTTT CTTTTGTAAT AACTTTATAT TAAAATTGAA AGTTTTAAAT
    GGAAGAGAAC AATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842186.1
    CDS281..6778
    Translation

    Target ORF information:

    RefSeq Version XM_024986418.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986418.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG ATTGTCGATA CAATAGAGAA
    GGAGAACAGG AGATGCCTGT TGCACAAGGT CATGATGAGG AGGAGGAGGA GGAGGAAGCA
    GAAGATGAGG CCCTCAGTGG AGCTTCACTG TCTAGTCACA GCAGCAGCCT GATCATTGAG
    ACTCCTGAAG ATGCTGAATT TGAACATAAG ATCCAGAAGC TTATGGCTGC AAAACTGAAA
    CTTAGACACT TACAGAACCT TGTAGCCATG GTGCAGGACG ATGATGCAGC CAATCAAGGA
    GTTACTTCTG TGGAGGATTT CTTCCCAGCA GAAGACACCA AACAAAATGC AAATAACACT
    AGAGGAAATG CCAATAAAAC ACAAAAAGAT GCTAGAGTAA ATGAAAAGGC AAGAGAGAAA
    TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAACTCA AACAATTGCA GGAAGAAAGA
    AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGAATTGC AAAAGGCATG TCCTAACCTA
    CAGCTGTCAG CTGCTAGTGT GGGTAATTGT CCCACAAAAA CACATATGCC AGCTGTTACT
    TCATCCCCAA CCGTTAATGA GAATGAAGCC AGTACAAACA AATCTGGTTT TGAGCCTCAT
    GATTCTTCAG TGGTAGATAA TGAGGTATGG TCAGATATGC GAAGACATGA AATGTTAAGG
    GAGGAGCTAC GACAGAGAAG AAAGCAGCTG GAAGCTCTGA TGGCTGAACA TCAGAGGAGG
    CAAGGTCTGG CTGAAACTGC ATCTCCAGTG GCTGTTTCAC TGAGAAGTGA TGGATCTGAG
    AACCTGTGTA CACCTCAGCA AAGCAGAACA GAAAAAACAA TGGCAACTTG GGGAGGTTCT
    ACCCAGTGTG CCCTAGATGA AGAAGGGGAT GAAGATGGTT ACCTTTCTGA AGGAATTATT
    CGGACAGACG AAGAGGAGGA AGAGGAGGAG CAAGATGCCA GTTCCAATGA TAACTTTCAT
    ATGTATCCTC CAAACAGTGC AAATCGTAAT TCTTACAATG TAAAGGAAAC TAAAAATAGG
    TGGAAGAACA ATCGCCCTTT TTCAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA
    CAACAGAATA TCAGCATGCA GCGTCAGGAA AACCTTCGCT GGGTGTCCGA ACTCTCTTAT
    GTAGAAGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TAAAGAAACA ACTTGATTTC
    AGTGTCAATA TTTGTCAGAC TTTAATGCAA GACCAGCAGA CTCTGTCTTG TCTGCTACAA
    ACTCTTCTCA CAAGTCCTTA TAGTGTTATG CCCAGCAGTG TTGCATCCCC GCAAGTGCAC
    CTTATAATGC ACCAGTTGAA CCAGTGCTAT ACCCAGCTAA CGTGGCAGCA GAATAATGTA
    CAGAGGTTGA AACAAATGCT CAGTGAACTC ATGCACCAAC AAAATCAGCA TCCAGAAAAA
    CCTGCAAGCA AGGAAGGAGG CAGTGGTGCA TCACACCCTC CTTCTCCCAG TTTATTTTGT
    CCTTTCAACT TTCCAACTCA GCCTGTAAAT CTGTTTAACC TACCGGGATT TACTAATTTT
    TCGTCATTTG CACCAAGTAT GAATTTCAGC CCTTTATTTC CTTCTAATTT TGGAGATTTT
    TCTCAAAATA TTTCTACACC CACTGAACAG CAGCAACCGT TAGCCCAGAA TCCTTCAGGG
    AAAACAGAAT ACATGGCTTT TCCAAAACCT TTTGAAAGTA GTTCTTCTAT TGGAGCAGAA
    AAACAAAGGA ATCAAAAACA GCCTGAAGAG GAGGTGGAGA ACAGTAGGAC ACCATGGTTT
    TATGACCAAG AAGGTGAGGT AGAAAAGCCA TTTCTCAAGA CTGGATTTGC AGTGTCTGTA
    GAGAAAACAA CAAATAGTAA TCGCAAAAAT CAACTAGATA CCAGTAGGAG AAGACGCCAG
    TTTGATGAAG TGTCCTTAGA AAGCTTTAAC AGTATGCTTG ACCCAGTAGA TCCAACAACA
    GTAACTAAAA CATTCAAGTC AAGAAAAGCA TCTGCACAGG CCAGCCTGGC ATCTAAAGAT
    AAAACTCCCA AATCAAAGAG TAAGAAGAGG CATTCTGCTC AGCCGAAAAG CAGAGTGAAA
    AATATTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGAAAC
    AGACATTCAG CCCAGACCGA AGAGCCTGTT CAAGCAAAAG TACTCAGCAG AAAGAAACAT
    GAGCAGCTGG AAAAAGTCAT AAGATGTAGT AGGTCTACAG AAATATCTTC AGCACATGCT
    AGGAGAATAC TACAGCAGTC TAACAGAAAT GCATGCAATG AAGCGCCAGA AACCAGTAGT
    GATTTTTCCA TGTTCGAAGC TTTGCGGGAT ACCATTTATT CTGAAGTAGC TACGTTGATT
    TCTCAAAATG AATCTCGTCC ACATTTTCTT ATTGAACTCT TCCATGAGCT TCAGCTACTT
    AATACAGACT ATTTGAGACA GAGGGCCTTA TATGCATTGC AGGACATAGT ATCCAGGCAT
    ATTTCTGAGA ACCATGAAAA AGAAGGAGAA AATATAAAGT CCGTGAATTC TGGTACTTGG
    ATAGCATCCA ACTCAGAACT TACTCCCAGT GAAAGCCTTG CTACTACTGA TGATGAAACT
    TTTGAGAAGA ACTTTGAGAG AGAAACACAT AAAATAAGTG AGCAAAATGA TGCTGATAAT
    GCTAGTGTAT CTTCAACTTG TGAGCCTTTT GCAACAGATG TTCTGGGTGA CACTGTGATT
    CACTTAGATC AGGCATTAGC CAGAATGAGA GACTACACGC ATATGAAGAT GGAGGCTGAA
    AGTAGTCCAG ACGTCAGGTG CACCTGCAGG ATCGTTGAGG ATGACGATGG TGCTGCTGCC
    ACGACCACGA TTAGTAATAA TTTAGAAGAA ACTCCTGTTA TTGAAAATCA TAGTTCCCAG
    CAACCCGTGA GTGAAGTTGC TACCGTCCCG TGTCCTAGAA TTGATACTCA GCAACTGGAC
    CGGCAAATTA AAGCAATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGAGCA CATGGATGAA
    GTATGTTCTT CTCAACTTCT AACCTCAGTA AGACGCATGG TTTTGGTCCT TACTCAGCAA
    AACAATGAGA GCAAAGAGTT TGTAAAGTTC TTTCATAAGC AGCTTGGAAG TATATTACAG
    GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAGGATT GTGGAGAAGA TCTTCTTGTA
    GAGATATCTG AAGTGTTATT TAATGAATTG GCTTTCTTTA AGCTCATGCA AGATTTGGAT
    AATAATAGTG TAACTGTTAA ACAGAGATGC AAAAGGAAAA TAGAAGCTGC TGGAGTGATA
    CAGTCTTATG CAAAAGAGGC CAAAAGGATT CTTGAAGGAG ATCACAGTTC ACCTGCTGGA
    GAAATTGATG ATGAAGACAA AGACAAGGAT GAGACGGAAA CAGTTAAGCA GACTCAAACG
    TCTGAGGTTT ATGATGGTGA CGGCCCCAAA AATGTGAGAT CTGATATTTC TGATCAAGAG
    GATGATGAAG AAAGTGAAGA ATGTCCAGTG TCTATTAGTT TGTCCAAAGC TGAAACTCAG
    GCTTTGACTA ATTACGGAAG TGGAGAAGAT GAGAATGAAG ATGAAGAGAT AGAAGAATTT
    GAAGAGGGAC CTGTAGATGT CCAGACGTCA CTTCAGGCTA ACACTGAAAC TACAGAAGAA
    AATGAACACG ATGATCAGGT TCCACAGCAT GATTTTGAAA AATCATCAGA AAGCAAAAAT
    GTCCCATCAG AACAAGAACC CACTACTAGT AAAGACTTTC TTTTGATGAC AGGTGACCAG
    GATAGCACTC CTGTGAAGCC ATGTTACCTC AATATCGTGG AAAATGAGCA ACCTTTAAAT
    AGCCCTGTCC AGAAGGACAC TCTCACTACC ATCGACTCGT CCAGTCAACC TAACCCTTTG
    CCTTTACCTT TAACTGAAAT TGAAACCTTG GTGCCTAGAG TCAAAGAAGT GAAATCTGCT
    CAGGAGACTC CTGAAAGTTC TTTGGCTGGA AGTCCTGATA CTGAATCTCC AGTGTTAGTG
    AATGACTATG AAGCAGAATC TGGTAACATA AGTCAAAAAT CTGATGAAGA AGACTTTGTG
    AAAGTTGAAG ATTTACCACT TAAACTGACA ATATATTCAG AGGCAGATCT AAGGAAGAAA
    ATGGCCGAAG AAGAACAGAA AAACCACTTA TCTGGTGAAA TATGTGCAAT GCAGACTGAA
    GAATTAGCTG GGAATTCTCA GACACTAAAA GAACCTGAGC AAGTATGTGC TCAGTCTGCC
    ATCTTAAGAT GGAAACCCTT ACCTATGCCT TTTAAACTGT TGGTGGTGTC GTCAGGGAAT
    TATTGTAGCA AAGTAAGTTT CTTTTGTAAT AACTTTATAT TAAAATTGAA AGTTTTAAAT
    GGAAGAGAAC AATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231464
    Clone ID Related Accession (Same CDS sequence) XM_024986419.1
    Accession Version XM_024986419.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG TATGCTTGAC CCAGTAGATC CAACAACAGT AACTAAAACA
    TTCAAGTCAA GAAAAGCATC TGCACAGGCC AGCCTGGCAT CTAAAGATAA AACTCCCAAA
    TCAAAGAGTA AGAAGAGGCA TTCTGCTCAG CCGAAAAGCA GAGTGAAAAA TATTGGGTAT
    GAAAGTGCCA GTGTGTCTAG CACATGTGAA CCTTGCAAAA GTAGAAACAG ACATTCAGCC
    CAGACCGAAG AGCCTGTTCA AGCAAAAGTA CTCAGCAGAA AGAAACATGA GCAGCTGGAA
    AAAGTCATAA GATGTAGTAG GTCTACAGAA ATATCTTCAG CACATGCTAG GAGAATACTA
    CAGCAGTCTA ACAGAAATGC ATGCAATGAA GCGCCAGAAA CCAGTAGTGA TTTTTCCATG
    TTCGAAGCTT TGCGGGATAC CATTTATTCT GAAGTAGCTA CGTTGATTTC TCAAAATGAA
    TCTCGTCCAC ATTTTCTTAT TGAACTCTTC CATGAGCTTC AGCTACTTAA TACAGACTAT
    TTGAGACAGA GGGCCTTATA TGCATTGCAG GACATAGTAT CCAGGCATAT TTCTGAGAAC
    CATGAAAAAG AAGGAGAAAA TATAAAGTCC GTGAATTCTG GTACTTGGAT AGCATCCAAC
    TCAGAACTTA CTCCCAGTGA AAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC
    TTTGAGAGAG AAACACATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTATCT
    TCAACTTGTG AGCCTTTTGC AACAGATGTT CTGGGTGACA CTGTGATTCA CTTAGATCAG
    GCATTAGCCA GAATGAGAGA CTACACGCAT ATGAAGATGG AGGCTGAAAG TAGTCCAGAC
    GTCAGGTGCA CCTGCAGGAT CGTTGAGGAT GACGATGGTG CTGCTGCCAC GACCACGATT
    AGTAATAATT TAGAAGAAAC TCCTGTTATT GAAAATCATA GTTCCCAGCA ACCCGTGAGT
    GAAGTTGCTA CCGTCCCGTG TCCTAGAATT GATACTCAGC AACTGGACCG GCAAATTAAA
    GCAATTATGA AAGAAGTCAT TCCTTTTTTG AAGGAGCACA TGGATGAAGT ATGTTCTTCT
    CAACTTCTAA CCTCAGTAAG ACGCATGGTT TTGGTCCTTA CTCAGCAAAA CAATGAGAGC
    AAAGAGTTTG TAAAGTTCTT TCATAAGCAG CTTGGAAGTA TATTACAGGA TTCACTGGCA
    AAATTTGCTG GCAGAAAACT GAAGGATTGT GGAGAAGATC TTCTTGTAGA GATATCTGAA
    GTGTTATTTA ATGAATTGGC TTTCTTTAAG CTCATGCAAG ATTTGGATAA TAATAGTGTA
    ACTGTTAAAC AGAGATGCAA AAGGAAAATA GAAGCTGCTG GAGTGATACA GTCTTATGCA
    AAAGAGGCCA AAAGGATTCT TGAAGGAGAT CACAGTTCAC CTGCTGGAGA AATTGATGAT
    GAAGACAAAG ACAAGGATGA GACGGAAACA GTTAAGCAGA CTCAAACGTC TGAGGTTTAT
    GATGGTGACG GCCCCAAAAA TGTGAGATCT GATATTTCTG ATCAAGAGGA TGATGAAGAA
    AGTGAAGAAT GTCCAGTGTC TATTAGTTTG TCCAAAGCTG AAACTCAGGC TTTGACTAAT
    TACGGAAGTG GAGAAGATGA GAATGAAGAT GAAGAGATAG AAGAATTTGA AGAGGGACCT
    GTAGATGTCC AGACGTCACT TCAGGCTAAC ACTGAAACTA CAGAAGAAAA TGAACACGAT
    GATCAGGTTC CACAGCATGA TTTTGAAAAA TCATCAGAAA GCAAAAATGT CCCATCAGAA
    CAAGAACCCA CTACTAGTAA AGACTTTCTT TTGATGACAG GTGACCAGGA TAGCACTCCT
    GTGAAGCCAT GTTACCTCAA TATCGTGGAA AATGAGCAAC CTTTAAATAG CCCTGTCCAG
    AAGGACACTC TCACTACCAT CGACTCGTCC AGTCAACCTA ACCCTTTGCC TTTACCTTTA
    ACTGAAATTG AAACCTTGGT GCCTAGAGTC AAAGAAGTGA AATCTGCTCA GGAGACTCCT
    GAAAGTTCTT TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA
    GCAGAATCTG GTAACATAAG TCAAAAATCT GATGAAGAAG ACTTTGTGAA AGTTGAAGAT
    TTACCACTTA AACTGACAAT ATATTCAGAG GCAGATCTAA GGAAGAAAAT GGCCGAAGAA
    GAACAGAAAA ACCACTTATC TGGTGAAATA TGTGCAATGC AGACTGAAGA ATTAGCTGGG
    AATTCTCAGA CACTAAAAGA ACCTGAGCAA GTATGTGCTC AGTCTGCCAT CTTAAGATGG
    AAACCCTTAC CTATGCCTTT TAAACTGTTG GTGGTGTCGT CAGGGAATTA TTGTAGCAAA
    GTAAGTTTCT TTTGTAATAA CTTTATATTA AAATTGAAAG TTTTAAATGG AAGAGAACAA
    TATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842187.1
    CDS359..6844
    Translation

    Target ORF information:

    RefSeq Version XM_024986419.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986419.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG TATGCTTGAC CCAGTAGATC CAACAACAGT AACTAAAACA
    TTCAAGTCAA GAAAAGCATC TGCACAGGCC AGCCTGGCAT CTAAAGATAA AACTCCCAAA
    TCAAAGAGTA AGAAGAGGCA TTCTGCTCAG CCGAAAAGCA GAGTGAAAAA TATTGGGTAT
    GAAAGTGCCA GTGTGTCTAG CACATGTGAA CCTTGCAAAA GTAGAAACAG ACATTCAGCC
    CAGACCGAAG AGCCTGTTCA AGCAAAAGTA CTCAGCAGAA AGAAACATGA GCAGCTGGAA
    AAAGTCATAA GATGTAGTAG GTCTACAGAA ATATCTTCAG CACATGCTAG GAGAATACTA
    CAGCAGTCTA ACAGAAATGC ATGCAATGAA GCGCCAGAAA CCAGTAGTGA TTTTTCCATG
    TTCGAAGCTT TGCGGGATAC CATTTATTCT GAAGTAGCTA CGTTGATTTC TCAAAATGAA
    TCTCGTCCAC ATTTTCTTAT TGAACTCTTC CATGAGCTTC AGCTACTTAA TACAGACTAT
    TTGAGACAGA GGGCCTTATA TGCATTGCAG GACATAGTAT CCAGGCATAT TTCTGAGAAC
    CATGAAAAAG AAGGAGAAAA TATAAAGTCC GTGAATTCTG GTACTTGGAT AGCATCCAAC
    TCAGAACTTA CTCCCAGTGA AAGCCTTGCT ACTACTGATG ATGAAACTTT TGAGAAGAAC
    TTTGAGAGAG AAACACATAA AATAAGTGAG CAAAATGATG CTGATAATGC TAGTGTATCT
    TCAACTTGTG AGCCTTTTGC AACAGATGTT CTGGGTGACA CTGTGATTCA CTTAGATCAG
    GCATTAGCCA GAATGAGAGA CTACACGCAT ATGAAGATGG AGGCTGAAAG TAGTCCAGAC
    GTCAGGTGCA CCTGCAGGAT CGTTGAGGAT GACGATGGTG CTGCTGCCAC GACCACGATT
    AGTAATAATT TAGAAGAAAC TCCTGTTATT GAAAATCATA GTTCCCAGCA ACCCGTGAGT
    GAAGTTGCTA CCGTCCCGTG TCCTAGAATT GATACTCAGC AACTGGACCG GCAAATTAAA
    GCAATTATGA AAGAAGTCAT TCCTTTTTTG AAGGAGCACA TGGATGAAGT ATGTTCTTCT
    CAACTTCTAA CCTCAGTAAG ACGCATGGTT TTGGTCCTTA CTCAGCAAAA CAATGAGAGC
    AAAGAGTTTG TAAAGTTCTT TCATAAGCAG CTTGGAAGTA TATTACAGGA TTCACTGGCA
    AAATTTGCTG GCAGAAAACT GAAGGATTGT GGAGAAGATC TTCTTGTAGA GATATCTGAA
    GTGTTATTTA ATGAATTGGC TTTCTTTAAG CTCATGCAAG ATTTGGATAA TAATAGTGTA
    ACTGTTAAAC AGAGATGCAA AAGGAAAATA GAAGCTGCTG GAGTGATACA GTCTTATGCA
    AAAGAGGCCA AAAGGATTCT TGAAGGAGAT CACAGTTCAC CTGCTGGAGA AATTGATGAT
    GAAGACAAAG ACAAGGATGA GACGGAAACA GTTAAGCAGA CTCAAACGTC TGAGGTTTAT
    GATGGTGACG GCCCCAAAAA TGTGAGATCT GATATTTCTG ATCAAGAGGA TGATGAAGAA
    AGTGAAGAAT GTCCAGTGTC TATTAGTTTG TCCAAAGCTG AAACTCAGGC TTTGACTAAT
    TACGGAAGTG GAGAAGATGA GAATGAAGAT GAAGAGATAG AAGAATTTGA AGAGGGACCT
    GTAGATGTCC AGACGTCACT TCAGGCTAAC ACTGAAACTA CAGAAGAAAA TGAACACGAT
    GATCAGGTTC CACAGCATGA TTTTGAAAAA TCATCAGAAA GCAAAAATGT CCCATCAGAA
    CAAGAACCCA CTACTAGTAA AGACTTTCTT TTGATGACAG GTGACCAGGA TAGCACTCCT
    GTGAAGCCAT GTTACCTCAA TATCGTGGAA AATGAGCAAC CTTTAAATAG CCCTGTCCAG
    AAGGACACTC TCACTACCAT CGACTCGTCC AGTCAACCTA ACCCTTTGCC TTTACCTTTA
    ACTGAAATTG AAACCTTGGT GCCTAGAGTC AAAGAAGTGA AATCTGCTCA GGAGACTCCT
    GAAAGTTCTT TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA
    GCAGAATCTG GTAACATAAG TCAAAAATCT GATGAAGAAG ACTTTGTGAA AGTTGAAGAT
    TTACCACTTA AACTGACAAT ATATTCAGAG GCAGATCTAA GGAAGAAAAT GGCCGAAGAA
    GAACAGAAAA ACCACTTATC TGGTGAAATA TGTGCAATGC AGACTGAAGA ATTAGCTGGG
    AATTCTCAGA CACTAAAAGA ACCTGAGCAA GTATGTGCTC AGTCTGCCAT CTTAAGATGG
    AAACCCTTAC CTATGCCTTT TAAACTGTTG GTGGTGTCGT CAGGGAATTA TTGTAGCAAA
    GTAAGTTTCT TTTGTAATAA CTTTATATTA AAATTGAAAG TTTTAAATGG AAGAGAACAA
    TATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231465
    Clone ID Related Accession (Same CDS sequence) XM_024986420.1
    Accession Version XM_024986420.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGGTGACCA GGATAGCACT
    CCTGTGAAGC CATGTTACCT CAATATCGTG GAAAATGAGC AACCTTTAAA TAGCCCTGTC
    CAGAAGGACA CTCTCACTAC CATCGACTCG TCCAGTCAAC CTAACCCTTT GCCTTTACCT
    TTAACTGAAA TTGAAACCTT GGTGCCTAGA GTCAAAGAAG TGAAATCTGC TCAGGAGACT
    CCTGAAAGTT CTTTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT
    GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA
    GATTTACCAC TTAAACTGAC AATATATTCA GAGGCAGATC TAAGGAAGAA AATGGCCGAA
    GAAGAACAGA AAAACCACTT ATCTGGTGAA ATATGTGCAA TGCAGACTGA AGAATTAGCT
    GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGTAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842188.1
    CDS359..6832
    Translation

    Target ORF information:

    RefSeq Version XM_024986420.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986420.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGGTGACCA GGATAGCACT
    CCTGTGAAGC CATGTTACCT CAATATCGTG GAAAATGAGC AACCTTTAAA TAGCCCTGTC
    CAGAAGGACA CTCTCACTAC CATCGACTCG TCCAGTCAAC CTAACCCTTT GCCTTTACCT
    TTAACTGAAA TTGAAACCTT GGTGCCTAGA GTCAAAGAAG TGAAATCTGC TCAGGAGACT
    CCTGAAAGTT CTTTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT
    GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA
    GATTTACCAC TTAAACTGAC AATATATTCA GAGGCAGATC TAAGGAAGAA AATGGCCGAA
    GAAGAACAGA AAAACCACTT ATCTGGTGAA ATATGTGCAA TGCAGACTGA AGAATTAGCT
    GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGTAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231466
    Clone ID Related Accession (Same CDS sequence) XM_024986421.1
    Accession Version XM_024986421.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6444bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGCAGATC TAAGGAAGAA AATGGCCGAA GAAGAACAGA AAAACCACTT ATCTGGTGAA
    ATATGTGCAA TGCAGACTGA AGAATTAGCT GGGAATTCTC AGACACTAAA AGAACCTGAA
    ACAGTGGGAG CCCAAAGTAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842189.1
    CDS359..6802
    Translation

    Target ORF information:

    RefSeq Version XM_024986421.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986421.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGCAGATC TAAGGAAGAA AATGGCCGAA GAAGAACAGA AAAACCACTT ATCTGGTGAA
    ATATGTGCAA TGCAGACTGA AGAATTAGCT GGGAATTCTC AGACACTAAA AGAACCTGAA
    ACAGTGGGAG CCCAAAGTAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231467
    Clone ID Related Accession (Same CDS sequence) XM_024986422.1
    Accession Version XM_024986422.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGCAGATTG TCGATACAAT
    AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG ATGAGGAGGA GGAGGAGGAG
    GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC
    ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA
    CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT
    CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT
    AACACTAGAG GAAATGCCAA TAAAACACAA AAAGATGCTA GAGTAAATGA AAAGGCAAGA
    GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA
    GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT
    AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT
    GTTACTTCAT CCCCAACCGT TAATGAGAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG
    CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG
    TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG
    AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA
    TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA
    GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGGGATGAAG ATGGTTACCT TTCTGAAGGA
    ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC
    TTTCATATGT ATCCTCCAAA CAGTGCAAAT CGTAATTCTT ACAATGTAAA GGAAACTAAA
    AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG
    ACAAGACAAC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC
    TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT
    GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG
    CTACAAACTC TTCTCACAAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA
    GTGCACCTTA TAATGCACCA GTTGAACCAG TGCTATACCC AGCTAACGTG GCAGCAGAAT
    AATGTACAGA GGTTGAAACA AATGCTCAGT GAACTCATGC ACCAACAAAA TCAGCATCCA
    GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTGCATCAC ACCCTCCTTC TCCCAGTTTA
    TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGATTTACT
    AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA
    GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT
    TCAGGGAAAA CAGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA
    GCAGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA
    TGGTTTTATG ACCAAGAAGG TGAGGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG
    TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA
    CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA
    ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT
    AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTGCTCAGCC GAAAAGCAGA
    GTGAAAAATA TTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT
    AGAAACAGAC ATTCAGCCCA GACCGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG
    AAACATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGCA
    CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACC
    AGTAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG
    TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG
    CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC
    AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT
    ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT
    GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT
    GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGTGACACT
    GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ACACGCATAT GAAGATGGAG
    GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCG TTGAGGATGA CGATGGTGCT
    GCTGCCACGA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT
    TCCCAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA
    CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG
    GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT
    CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA
    TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT
    CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT
    TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA
    GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT
    GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT
    CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TATTTCTGAT
    CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAGTTTGTC CAAAGCTGAA
    ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAGATAGAA
    GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA
    GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC
    AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG ACTTTCTTTT GATGACAGGT
    GACCAGGATA GCACTCCTGT GAAGCCATGT TACCTCAATA TCGTGGAAAA TGAGCAACCT
    TTAAATAGCC CTGTCCAGAA GGACACTCTC ACTACCATCG ACTCGTCCAG TCAACCTAAC
    CCTTTGCCTT TACCTTTAAC TGAAATTGAA ACCTTGGTGC CTAGAGTCAA AGAAGTGAAA
    TCTGCTCAGG AGACTCCTGA AAGTTCTTTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG
    TTAGTGAATG ACTATGAAGC AGAATCTGGT AACATAAGTC AAAAATCTGA TGAAGAAGAC
    TTTGTGAAAG TTGAAGATTT ACCACTTAAA CTGACAATAT ATTCAGAGGC AGATCTAAGG
    AAGAAAATGG CCGAAGAAGA ACAGAAAAAC CACTTATCTG GTGAAATATG TGCAATGCAG
    ACTGAAGAAT TAGCTGGGAA TTCTCAGACA CTAAAAGAAC CTGAGCAAGT ATGTGCTCAG
    TCTGCCATCT TAAGATGGAA ACCCTTACCT ATGCCTTTTA AACTGTTGGT GGTGTCGTCA
    GGGAATTATT GTAGCAAAGT AAGTTTCTTT TGTAATAACT TTATATTAAA ATTGAAAGTT
    TTAAATGGAA GAGAACAATA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842190.1
    CDS359..6742
    Translation

    Target ORF information:

    RefSeq Version XM_024986422.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986422.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGCAGATTG TCGATACAAT
    AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG ATGAGGAGGA GGAGGAGGAG
    GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC
    ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA
    CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT
    CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT
    AACACTAGAG GAAATGCCAA TAAAACACAA AAAGATGCTA GAGTAAATGA AAAGGCAAGA
    GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA
    GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT
    AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT
    GTTACTTCAT CCCCAACCGT TAATGAGAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG
    CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG
    TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG
    AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA
    TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA
    GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGGGATGAAG ATGGTTACCT TTCTGAAGGA
    ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC
    TTTCATATGT ATCCTCCAAA CAGTGCAAAT CGTAATTCTT ACAATGTAAA GGAAACTAAA
    AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG
    ACAAGACAAC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC
    TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT
    GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG
    CTACAAACTC TTCTCACAAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA
    GTGCACCTTA TAATGCACCA GTTGAACCAG TGCTATACCC AGCTAACGTG GCAGCAGAAT
    AATGTACAGA GGTTGAAACA AATGCTCAGT GAACTCATGC ACCAACAAAA TCAGCATCCA
    GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTGCATCAC ACCCTCCTTC TCCCAGTTTA
    TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGATTTACT
    AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA
    GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT
    TCAGGGAAAA CAGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA
    GCAGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA
    TGGTTTTATG ACCAAGAAGG TGAGGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG
    TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA
    CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA
    ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT
    AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTGCTCAGCC GAAAAGCAGA
    GTGAAAAATA TTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT
    AGAAACAGAC ATTCAGCCCA GACCGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG
    AAACATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGCA
    CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACC
    AGTAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG
    TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG
    CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC
    AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT
    ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT
    GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT
    GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGTGACACT
    GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ACACGCATAT GAAGATGGAG
    GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCG TTGAGGATGA CGATGGTGCT
    GCTGCCACGA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT
    TCCCAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA
    CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG
    GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT
    CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA
    TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT
    CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT
    TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA
    GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT
    GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT
    CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TATTTCTGAT
    CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAGTTTGTC CAAAGCTGAA
    ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAGATAGAA
    GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA
    GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC
    AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG ACTTTCTTTT GATGACAGGT
    GACCAGGATA GCACTCCTGT GAAGCCATGT TACCTCAATA TCGTGGAAAA TGAGCAACCT
    TTAAATAGCC CTGTCCAGAA GGACACTCTC ACTACCATCG ACTCGTCCAG TCAACCTAAC
    CCTTTGCCTT TACCTTTAAC TGAAATTGAA ACCTTGGTGC CTAGAGTCAA AGAAGTGAAA
    TCTGCTCAGG AGACTCCTGA AAGTTCTTTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG
    TTAGTGAATG ACTATGAAGC AGAATCTGGT AACATAAGTC AAAAATCTGA TGAAGAAGAC
    TTTGTGAAAG TTGAAGATTT ACCACTTAAA CTGACAATAT ATTCAGAGGC AGATCTAAGG
    AAGAAAATGG CCGAAGAAGA ACAGAAAAAC CACTTATCTG GTGAAATATG TGCAATGCAG
    ACTGAAGAAT TAGCTGGGAA TTCTCAGACA CTAAAAGAAC CTGAGCAAGT ATGTGCTCAG
    TCTGCCATCT TAAGATGGAA ACCCTTACCT ATGCCTTTTA AACTGTTGGT GGTGTCGTCA
    GGGAATTATT GTAGCAAAGT AAGTTTCTTT TGTAATAACT TTATATTAAA ATTGAAAGTT
    TTAAATGGAA GAGAACAATA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231468
    Clone ID Related Accession (Same CDS sequence) XM_024986423.1
    Accession Version XM_024986423.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG ATTGTCGATA CAATAGAGAA
    GGAGAACAGG AGATGCCTGT TGCACAAGGT CATGATGAGG AGGAGGAGGA GGAGGAAGCA
    GAAGATGAGG CCCTCAGTGG AGCTTCACTG TCTAGTCACA GCAGCAGCCT GATCATTGAG
    ACTCCTGAAG ATGCTGAATT TGAACATAAG ATCCAGAAGC TTATGGCTGC AAAACTGAAA
    CTTAGACACT TACAGAACCT TGTAGCCATG GTGCAGGACG ATGATGCAGC CAATCAAGGA
    GTTACTTCTG TGGAGGATTT CTTCCCAGCA GAAGACACCA AACAAAATGC AAATAACACT
    AGAGGAAATG CCAATAAAAC ACAAAAAGAT GCTAGAGTAA ATGAAAAGGC AAGAGAGAAA
    TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAACTCA AACAATTGCA GGAAGAAAGA
    AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGAATTGC AAAAGGCATG TCCTAACCTA
    CAGCTGTCAG CTGCTAGTGT GGGTAATTGT CCCACAAAAA CACATATGCC AGCTGTTACT
    TCATCCCCAA CCGTTAATGA GAATGAAGCC AGTACAAACA AATCTGGTTT TGAGCCTCAT
    GATTCTTCAG TGGTAGATAA TGAGGTATGG TCAGATATGC GAAGACATGA AATGTTAAGG
    GAGGAGCTAC GACAGAGAAG AAAGCAGCTG GAAGCTCTGA TGGCTGAACA TCAGAGGAGG
    CAAGGTCTGG CTGAAACTGC ATCTCCAGTG GCTGTTTCAC TGAGAAGTGA TGGATCTGAG
    AACCTGTGTA CACCTCAGCA AAGCAGAACA GAAAAAACAA TGGCAACTTG GGGAGGTTCT
    ACCCAGTGTG CCCTAGATGA AGAAGGGGAT GAAGATGGTT ACCTTTCTGA AGGAATTATT
    CGGACAGACG AAGAGGAGGA AGAGGAGGAG CAAGATGCCA GTTCCAATGA TAACTTTCAT
    ATGTATCCTC CAAACAGTGC AAATCGTAAT TCTTACAATG TAAAGGAAAC TAAAAATAGG
    TGGAAGAACA ATCGCCCTTT TTCAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA
    CAACAGAATA TCAGCATGCA GCGTCAGGAA AACCTTCGCT GGGTGTCCGA ACTCTCTTAT
    GTAGAAGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TAAAGAAACA ACTTGATTTC
    AGTGTCAATA TTTGTCAGAC TTTAATGCAA GACCAGCAGA CTCTGTCTTG TCTGCTACAA
    ACTCTTCTCA CAAGTCCTTA TAGTGTTATG CCCAGCAGTG TTGCATCCCC GCAAGTGCAC
    CTTATAATGC ACCAGTTGAA CCAGTGCTAT ACCCAGCTAA CGTGGCAGCA GAATAATGTA
    CAGAGGTTGA AACAAATGCT CAGTGAACTC ATGCACCAAC AAAATCAGCA TCCAGAAAAA
    CCTGCAAGCA AGGAAGGAGG CAGTGGTGCA TCACACCCTC CTTCTCCCAG TTTATTTTGT
    CCTTTCAACT TTCCAACTCA GCCTGTAAAT CTGTTTAACC TACCGGGATT TACTAATTTT
    TCGTCATTTG CACCAAGTAT GAATTTCAGC CCTTTATTTC CTTCTAATTT TGGAGATTTT
    TCTCAAAATA TTTCTACACC CACTGAACAG CAGCAACCGT TAGCCCAGAA TCCTTCAGGG
    AAAACAGAAT ACATGGCTTT TCCAAAACCT TTTGAAAGTA GTTCTTCTAT TGGAGCAGAA
    AAACAAAGGA ATCAAAAACA GCCTGAAGAG GAGGTGGAGA ACAGTAGGAC ACCATGGTTT
    TATGACCAAG AAGGTGAGGT AGAAAAGCCA TTTCTCAAGA CTGGATTTGC AGTGTCTGTA
    GAGAAAACAA CAAATAGTAA TCGCAAAAAT CAACTAGATA CCAGTAGGAG AAGACGCCAG
    TTTGATGAAG TGTCCTTAGA AAGCTTTAAC AGTATGCTTG ACCCAGTAGA TCCAACAACA
    GTAACTAAAA CATTCAAGTC AAGAAAAGCA TCTGCACAGG CCAGCCTGGC ATCTAAAGAT
    AAAACTCCCA AATCAAAGAG TAAGAAGAGG CATTCTGCTC AGCCGAAAAG CAGAGTGAAA
    AATATTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGAAAC
    AGACATTCAG CCCAGACCGA AGAGCCTGTT CAAGCAAAAG TACTCAGCAG AAAGAAACAT
    GAGCAGCTGG AAAAAGTCAT AAGATGTAGT AGGTCTACAG AAATATCTTC AGCACATGCT
    AGGAGAATAC TACAGCAGTC TAACAGAAAT GCATGCAATG AAGCGCCAGA AACCAGTAGT
    GATTTTTCCA TGTTCGAAGC TTTGCGGGAT ACCATTTATT CTGAAGTAGC TACGTTGATT
    TCTCAAAATG AATCTCGTCC ACATTTTCTT ATTGAACTCT TCCATGAGCT TCAGCTACTT
    AATACAGACT ATTTGAGACA GAGGGCCTTA TATGCATTGC AGGACATAGT ATCCAGGCAT
    ATTTCTGAGA ACCATGAAAA AGAAGGAGAA AATATAAAGT CCGTGAATTC TGGTACTTGG
    ATAGCATCCA ACTCAGAACT TACTCCCAGT GAAAGCCTTG CTACTACTGA TGATGAAACT
    TTTGAGAAGA ACTTTGAGAG AGAAACACAT AAAATAAGTG AGCAAAATGA TGCTGATAAT
    GCTAGTGTAT CTTCAACTTG TGAGCCTTTT GCAACAGATG TTCTGGGTGA CACTGTGATT
    CACTTAGATC AGGCATTAGC CAGAATGAGA GACTACACGC ATATGAAGAT GGAGGCTGAA
    AGTAGTCCAG ACGTCAGGTG CACCTGCAGG ATCGTTGAGG ATGACGATGG TGCTGCTGCC
    ACGACCACGA TTAGTAATAA TTTAGAAGAA ACTCCTGTTA TTGAAAATCA TAGTTCCCAG
    CAACCCGTGA GTGAAGTTGC TACCGTCCCG TGTCCTAGAA TTGATACTCA GCAACTGGAC
    CGGCAAATTA AAGCAATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGAGCA CATGGATGAA
    GTATGTTCTT CTCAACTTCT AACCTCAGTA AGACGCATGG TTTTGGTCCT TACTCAGCAA
    AACAATGAGA GCAAAGAGTT TGTAAAGTTC TTTCATAAGC AGCTTGGAAG TATATTACAG
    GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAGGATT GTGGAGAAGA TCTTCTTGTA
    GAGATATCTG AAGTGTTATT TAATGAATTG GCTTTCTTTA AGCTCATGCA AGATTTGGAT
    AATAATAGTG TAACTGTTAA ACAGAGATGC AAAAGGAAAA TAGAAGCTGC TGGAGTGATA
    CAGTCTTATG CAAAAGAGGC CAAAAGGATT CTTGAAGGAG ATCACAGTTC ACCTGCTGGA
    GAAATTGATG ATGAAGACAA AGACAAGGAT GAGACGGAAA CAGTTAAGCA GACTCAAACG
    TCTGAGGTTT ATGATGGTGA CGGCCCCAAA AATGTGAGAT CTGATATTTC TGATCAAGAG
    GATGATGAAG AAAGTGAAGA ATGTCCAGTG TCTATTAGTT TGTCCAAAGC TGAAACTCAG
    GCTTTGACTA ATTACGGAAG TGGAGAAGAT GAGAATGAAG ATGAAGAGAT AGAAGAATTT
    GAAGAGGGAC CTGTAGATGT CCAGACGTCA CTTCAGGCTA ACACTGAAAC TACAGAAGAA
    AATGAACACG ATGATCAGGT TCCACAGCAT GATTTTGAAA AATCATCAGA AAGCAAAAAT
    GTCCCATCAG AACAAGAACC CACTACTAGT AAAGGTGACC AGGATAGCAC TCCTGTGAAG
    CCATGTTACC TCAATATCGT GGAAAATGAG CAACCTTTAA ATAGCCCTGT CCAGAAGGAC
    ACTCTCACTA CCATCGACTC GTCCAGTCAA CCTAACCCTT TGCCTTTACC TTTAACTGAA
    ATTGAAACCT TGGTGCCTAG AGTCAAAGAA GTGAAATCTG CTCAGGAGAC TCCTGAAAGT
    TCTTTGGCTG GAAGTCCTGA TACTGAATCT CCAGTGTTAG TGAATGACTA TGAAGCAGAA
    TCTGGTAACA TAAGTCAAAA ATCTGATGAA GAAGACTTTG TGAAAGTTGA AGATTTACCA
    CTTAAACTGA CAATATATTC AGAGGCAGAT CTAAGGAAGA AAATGGCCGA AGAAGAACAG
    AAAAACCACT TATCTGGTGA AATATGTGCA ATGCAGACTG AAGAATTAGC TGGGAATTCT
    CAGACACTAA AAGAACCTGA AACAGTGGGA GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842191.1
    CDS494..6838
    Translation

    Target ORF information:

    RefSeq Version XM_024986423.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986423.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG ATTGTCGATA CAATAGAGAA
    GGAGAACAGG AGATGCCTGT TGCACAAGGT CATGATGAGG AGGAGGAGGA GGAGGAAGCA
    GAAGATGAGG CCCTCAGTGG AGCTTCACTG TCTAGTCACA GCAGCAGCCT GATCATTGAG
    ACTCCTGAAG ATGCTGAATT TGAACATAAG ATCCAGAAGC TTATGGCTGC AAAACTGAAA
    CTTAGACACT TACAGAACCT TGTAGCCATG GTGCAGGACG ATGATGCAGC CAATCAAGGA
    GTTACTTCTG TGGAGGATTT CTTCCCAGCA GAAGACACCA AACAAAATGC AAATAACACT
    AGAGGAAATG CCAATAAAAC ACAAAAAGAT GCTAGAGTAA ATGAAAAGGC AAGAGAGAAA
    TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAACTCA AACAATTGCA GGAAGAAAGA
    AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGAATTGC AAAAGGCATG TCCTAACCTA
    CAGCTGTCAG CTGCTAGTGT GGGTAATTGT CCCACAAAAA CACATATGCC AGCTGTTACT
    TCATCCCCAA CCGTTAATGA GAATGAAGCC AGTACAAACA AATCTGGTTT TGAGCCTCAT
    GATTCTTCAG TGGTAGATAA TGAGGTATGG TCAGATATGC GAAGACATGA AATGTTAAGG
    GAGGAGCTAC GACAGAGAAG AAAGCAGCTG GAAGCTCTGA TGGCTGAACA TCAGAGGAGG
    CAAGGTCTGG CTGAAACTGC ATCTCCAGTG GCTGTTTCAC TGAGAAGTGA TGGATCTGAG
    AACCTGTGTA CACCTCAGCA AAGCAGAACA GAAAAAACAA TGGCAACTTG GGGAGGTTCT
    ACCCAGTGTG CCCTAGATGA AGAAGGGGAT GAAGATGGTT ACCTTTCTGA AGGAATTATT
    CGGACAGACG AAGAGGAGGA AGAGGAGGAG CAAGATGCCA GTTCCAATGA TAACTTTCAT
    ATGTATCCTC CAAACAGTGC AAATCGTAAT TCTTACAATG TAAAGGAAAC TAAAAATAGG
    TGGAAGAACA ATCGCCCTTT TTCAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA
    CAACAGAATA TCAGCATGCA GCGTCAGGAA AACCTTCGCT GGGTGTCCGA ACTCTCTTAT
    GTAGAAGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TAAAGAAACA ACTTGATTTC
    AGTGTCAATA TTTGTCAGAC TTTAATGCAA GACCAGCAGA CTCTGTCTTG TCTGCTACAA
    ACTCTTCTCA CAAGTCCTTA TAGTGTTATG CCCAGCAGTG TTGCATCCCC GCAAGTGCAC
    CTTATAATGC ACCAGTTGAA CCAGTGCTAT ACCCAGCTAA CGTGGCAGCA GAATAATGTA
    CAGAGGTTGA AACAAATGCT CAGTGAACTC ATGCACCAAC AAAATCAGCA TCCAGAAAAA
    CCTGCAAGCA AGGAAGGAGG CAGTGGTGCA TCACACCCTC CTTCTCCCAG TTTATTTTGT
    CCTTTCAACT TTCCAACTCA GCCTGTAAAT CTGTTTAACC TACCGGGATT TACTAATTTT
    TCGTCATTTG CACCAAGTAT GAATTTCAGC CCTTTATTTC CTTCTAATTT TGGAGATTTT
    TCTCAAAATA TTTCTACACC CACTGAACAG CAGCAACCGT TAGCCCAGAA TCCTTCAGGG
    AAAACAGAAT ACATGGCTTT TCCAAAACCT TTTGAAAGTA GTTCTTCTAT TGGAGCAGAA
    AAACAAAGGA ATCAAAAACA GCCTGAAGAG GAGGTGGAGA ACAGTAGGAC ACCATGGTTT
    TATGACCAAG AAGGTGAGGT AGAAAAGCCA TTTCTCAAGA CTGGATTTGC AGTGTCTGTA
    GAGAAAACAA CAAATAGTAA TCGCAAAAAT CAACTAGATA CCAGTAGGAG AAGACGCCAG
    TTTGATGAAG TGTCCTTAGA AAGCTTTAAC AGTATGCTTG ACCCAGTAGA TCCAACAACA
    GTAACTAAAA CATTCAAGTC AAGAAAAGCA TCTGCACAGG CCAGCCTGGC ATCTAAAGAT
    AAAACTCCCA AATCAAAGAG TAAGAAGAGG CATTCTGCTC AGCCGAAAAG CAGAGTGAAA
    AATATTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGAAAC
    AGACATTCAG CCCAGACCGA AGAGCCTGTT CAAGCAAAAG TACTCAGCAG AAAGAAACAT
    GAGCAGCTGG AAAAAGTCAT AAGATGTAGT AGGTCTACAG AAATATCTTC AGCACATGCT
    AGGAGAATAC TACAGCAGTC TAACAGAAAT GCATGCAATG AAGCGCCAGA AACCAGTAGT
    GATTTTTCCA TGTTCGAAGC TTTGCGGGAT ACCATTTATT CTGAAGTAGC TACGTTGATT
    TCTCAAAATG AATCTCGTCC ACATTTTCTT ATTGAACTCT TCCATGAGCT TCAGCTACTT
    AATACAGACT ATTTGAGACA GAGGGCCTTA TATGCATTGC AGGACATAGT ATCCAGGCAT
    ATTTCTGAGA ACCATGAAAA AGAAGGAGAA AATATAAAGT CCGTGAATTC TGGTACTTGG
    ATAGCATCCA ACTCAGAACT TACTCCCAGT GAAAGCCTTG CTACTACTGA TGATGAAACT
    TTTGAGAAGA ACTTTGAGAG AGAAACACAT AAAATAAGTG AGCAAAATGA TGCTGATAAT
    GCTAGTGTAT CTTCAACTTG TGAGCCTTTT GCAACAGATG TTCTGGGTGA CACTGTGATT
    CACTTAGATC AGGCATTAGC CAGAATGAGA GACTACACGC ATATGAAGAT GGAGGCTGAA
    AGTAGTCCAG ACGTCAGGTG CACCTGCAGG ATCGTTGAGG ATGACGATGG TGCTGCTGCC
    ACGACCACGA TTAGTAATAA TTTAGAAGAA ACTCCTGTTA TTGAAAATCA TAGTTCCCAG
    CAACCCGTGA GTGAAGTTGC TACCGTCCCG TGTCCTAGAA TTGATACTCA GCAACTGGAC
    CGGCAAATTA AAGCAATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGAGCA CATGGATGAA
    GTATGTTCTT CTCAACTTCT AACCTCAGTA AGACGCATGG TTTTGGTCCT TACTCAGCAA
    AACAATGAGA GCAAAGAGTT TGTAAAGTTC TTTCATAAGC AGCTTGGAAG TATATTACAG
    GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAGGATT GTGGAGAAGA TCTTCTTGTA
    GAGATATCTG AAGTGTTATT TAATGAATTG GCTTTCTTTA AGCTCATGCA AGATTTGGAT
    AATAATAGTG TAACTGTTAA ACAGAGATGC AAAAGGAAAA TAGAAGCTGC TGGAGTGATA
    CAGTCTTATG CAAAAGAGGC CAAAAGGATT CTTGAAGGAG ATCACAGTTC ACCTGCTGGA
    GAAATTGATG ATGAAGACAA AGACAAGGAT GAGACGGAAA CAGTTAAGCA GACTCAAACG
    TCTGAGGTTT ATGATGGTGA CGGCCCCAAA AATGTGAGAT CTGATATTTC TGATCAAGAG
    GATGATGAAG AAAGTGAAGA ATGTCCAGTG TCTATTAGTT TGTCCAAAGC TGAAACTCAG
    GCTTTGACTA ATTACGGAAG TGGAGAAGAT GAGAATGAAG ATGAAGAGAT AGAAGAATTT
    GAAGAGGGAC CTGTAGATGT CCAGACGTCA CTTCAGGCTA ACACTGAAAC TACAGAAGAA
    AATGAACACG ATGATCAGGT TCCACAGCAT GATTTTGAAA AATCATCAGA AAGCAAAAAT
    GTCCCATCAG AACAAGAACC CACTACTAGT AAAGGTGACC AGGATAGCAC TCCTGTGAAG
    CCATGTTACC TCAATATCGT GGAAAATGAG CAACCTTTAA ATAGCCCTGT CCAGAAGGAC
    ACTCTCACTA CCATCGACTC GTCCAGTCAA CCTAACCCTT TGCCTTTACC TTTAACTGAA
    ATTGAAACCT TGGTGCCTAG AGTCAAAGAA GTGAAATCTG CTCAGGAGAC TCCTGAAAGT
    TCTTTGGCTG GAAGTCCTGA TACTGAATCT CCAGTGTTAG TGAATGACTA TGAAGCAGAA
    TCTGGTAACA TAAGTCAAAA ATCTGATGAA GAAGACTTTG TGAAAGTTGA AGATTTACCA
    CTTAAACTGA CAATATATTC AGAGGCAGAT CTAAGGAAGA AAATGGCCGA AGAAGAACAG
    AAAAACCACT TATCTGGTGA AATATGTGCA ATGCAGACTG AAGAATTAGC TGGGAATTCT
    CAGACACTAA AAGAACCTGA AACAGTGGGA GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231469
    Clone ID Related Accession (Same CDS sequence) XM_024986424.1
    Accession Version XM_024986424.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6288bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG ATTGTCGATA CAATAGAGAA
    GGAGAACAGG AGATGCCTGT TGCACAAGGT CATGATGAGG AGGAGGAGGA GGAGGAAGCA
    GAAGATGAGG CCCTCAGTGG AGCTTCACTG TCTAGTCACA GCAGCAGCCT GATCATTGAG
    ACTCCTGAAG ATGCTGAATT TGAACATAAG ATCCAGAAGC TTATGGCTGC AAAACTGAAA
    CTTAGACACT TACAGAACCT TGTAGCCATG GTGCAGGACG ATGATGCAGC CAATCAAGGA
    GTTACTTCTG TGGAGGATTT CTTCCCAGCA GAAGACACCA AACAAAATGC AAATAACACT
    AGAGGAAATG CCAATAAAAC ACAAAAAGAT GCTAGAGTAA ATGAAAAGGC AAGAGAGAAA
    TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAACTCA AACAATTGCA GGAAGAAAGA
    AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGAATTGC AAAAGGCATG TCCTAACCTA
    CAGCTGTCAG CTGCTAGTGT GGGTAATTGT CCCACAAAAA CACATATGCC AGCTGTTACT
    TCATCCCCAA CCGTTAATGA GAATGAAGCC AGTACAAACA AATCTGGTTT TGAGCCTCAT
    GATTCTTCAG TGGTAGATAA TGAGGTATGG TCAGATATGC GAAGACATGA AATGTTAAGG
    GAGGAGCTAC GACAGAGAAG AAAGCAGCTG GAAGCTCTGA TGGCTGAACA TCAGAGGAGG
    CAAGGTCTGG CTGAAACTGC ATCTCCAGTG GCTGTTTCAC TGAGAAGTGA TGGATCTGAG
    AACCTGTGTA CACCTCAGCA AAGCAGAACA GAAAAAACAA TGGCAACTTG GGGAGGTTCT
    ACCCAGTGTG CCCTAGATGA AGAAGGGGAT GAAGATGGTT ACCTTTCTGA AGGAATTATT
    CGGACAGACG AAGAGGAGGA AGAGGAGGAG CAAGATGCCA GTTCCAATGA TAACTTTCAT
    ATGTATCCTC CAAACAGTGC AAATCGTAAT TCTTACAATG TAAAGGAAAC TAAAAATAGG
    TGGAAGAACA ATCGCCCTTT TTCAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA
    CAACAGAATA TCAGCATGCA GCGTCAGGAA AACCTTCGCT GGGTGTCCGA ACTCTCTTAT
    GTAGAAGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TAAAGAAACA ACTTGATTTC
    AGTGTCAATA TTTGTCAGAC TTTAATGCAA GACCAGCAGA CTCTGTCTTG TCTGCTACAA
    ACTCTTCTCA CAAGTCCTTA TAGTGTTATG CCCAGCAGTG TTGCATCCCC GCAAGTGCAC
    CTTATAATGC ACCAGTTGAA CCAGTGCTAT ACCCAGCTAA CGTGGCAGCA GAATAATGTA
    CAGAGGTTGA AACAAATGCT CAGTGAACTC ATGCACCAAC AAAATCAGCA TCCAGAAAAA
    CCTGCAAGCA AGGAAGGAGG CAGTGGTGCA TCACACCCTC CTTCTCCCAG TTTATTTTGT
    CCTTTCAACT TTCCAACTCA GCCTGTAAAT CTGTTTAACC TACCGGGATT TACTAATTTT
    TCGTCATTTG CACCAAGTAT GAATTTCAGC CCTTTATTTC CTTCTAATTT TGGAGATTTT
    TCTCAAAATA TTTCTACACC CACTGAACAG CAGCAACCGT TAGCCCAGAA TCCTTCAGGG
    AAAACAGAAT ACATGGCTTT TCCAAAACCT TTTGAAAGTA GTTCTTCTAT TGGAGCAGAA
    AAACAAAGGA ATCAAAAACA GCCTGAAGAG GAGGTGGAGA ACAGTAGGAC ACCATGGTTT
    TATGACCAAG AAGGTGAGGT AGAAAAGCCA TTTCTCAAGA CTGGATTTGC AGTGTCTGTA
    GAGAAAACAA CAAATAGTAA TCGCAAAAAT CAACTAGATA CCAGTAGGAG AAGACGCCAG
    TTTGATGAAG TGTCCTTAGA AAGCTTTAAC AGTATGCTTG ACCCAGTAGA TCCAACAACA
    GTAACTAAAA CATTCAAGTC AAGAAAAGCA TCTGCACAGG CCAGCCTGGC ATCTAAAGAT
    AAAACTCCCA AATCAAAGAG TAAGAAGAGG CATTCTGCTC AGCCGAAAAG CAGAGTGAAA
    AATATTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGAAAC
    AGACATTCAG CCCAGACCGA AGAGCCTGTT CAAGCAAAAG TACTCAGCAG AAAGAAACAT
    GAGCAGCTGG AAAAAGTCAT AAGATGTAGT AGGTCTACAG AAATATCTTC AGAAACCAGT
    AGTGATTTTT CCATGTTCGA AGCTTTGCGG GATACCATTT ATTCTGAAGT AGCTACGTTG
    ATTTCTCAAA ATGAATCTCG TCCACATTTT CTTATTGAAC TCTTCCATGA GCTTCAGCTA
    CTTAATACAG ACTATTTGAG ACAGAGGGCC TTATATGCAT TGCAGGACAT AGTATCCAGG
    CATATTTCTG AGAACCATGA AAAAGAAGGA GAAAATATAA AGTCCGTGAA TTCTGGTACT
    TGGATAGCAT CCAACTCAGA ACTTACTCCC AGTGAAAGCC TTGCTACTAC TGATGATGAA
    ACTTTTGAGA AGAACTTTGA GAGAGAAACA CATAAAATAA GTGAGCAAAA TGATGCTGAT
    AATGCTAGTG TATCTTCAAC TTGTGAGCCT TTTGCAACAG ATGTTCTGGG TGACACTGTG
    ATTCACTTAG ATCAGGCATT AGCCAGAATG AGAGACTACA CGCATATGAA GATGGAGGCT
    GAAAGTAGTC CAGACGTCAG GTGCACCTGC AGGATCGTTG AGGATGACGA TGGTGCTGCT
    GCCACGACCA CGATTAGTAA TAATTTAGAA GAAACTCCTG TTATTGAAAA TCATAGTTCC
    CAGCAACCCG TGAGTGAAGT TGCTACCGTC CCGTGTCCTA GAATTGATAC TCAGCAACTG
    GACCGGCAAA TTAAAGCAAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT
    GAAGTATGTT CTTCTCAACT TCTAACCTCA GTAAGACGCA TGGTTTTGGT CCTTACTCAG
    CAAAACAATG AGAGCAAAGA GTTTGTAAAG TTCTTTCATA AGCAGCTTGG AAGTATATTA
    CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAGG ATTGTGGAGA AGATCTTCTT
    GTAGAGATAT CTGAAGTGTT ATTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG
    GATAATAATA GTGTAACTGT TAAACAGAGA TGCAAAAGGA AAATAGAAGC TGCTGGAGTG
    ATACAGTCTT ATGCAAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCACAG TTCACCTGCT
    GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAGACGG AAACAGTTAA GCAGACTCAA
    ACGTCTGAGG TTTATGATGG TGACGGCCCC AAAAATGTGA GATCTGATAT TTCTGATCAA
    GAGGATGATG AAGAAAGTGA AGAATGTCCA GTGTCTATTA GTTTGTCCAA AGCTGAAACT
    CAGGCTTTGA CTAATTACGG AAGTGGAGAA GATGAGAATG AAGATGAAGA GATAGAAGAA
    TTTGAAGAGG GACCTGTAGA TGTCCAGACG TCACTTCAGG CTAACACTGA AACTACAGAA
    GAAAATGAAC ACGATGATCA GGTTCCACAG CATGATTTTG AAAAATCATC AGAAAGCAAA
    AATGTCCCAT CAGAACAAGA ACCCACTACT AGTAAAGGTG ACCAGGATAG CACTCCTGTG
    AAGCCATGTT ACCTCAATAT CGTGGAAAAT GAGCAACCTT TAAATAGCCC TGTCCAGAAG
    GACACTCTCA CTACCATCGA CTCGTCCAGT CAACCTAACC CTTTGCCTTT ACCTTTAACT
    GAAATTGAAA CCTTGGTGCC TAGAGTCAAA GAAGTGAAAT CTGCTCAGGA GACTCCTGAA
    AGTTCTTTGG CTGGAAGTCC TGATACTGAA TCTCCAGTGT TAGTGAATGA CTATGAAGCA
    GAATCTGGTA ACATAAGTCA AAAATCTGAT GAAGAAGACT TTGTGAAAGT TGAAGATTTA
    CCACTTAAAC TGACAATATA TTCAGAGGCA GATCTAAGGA AGAAAATGGC CGAAGAAGAA
    CAGAAAAACC ACTTATCTGG TGAAATATGT GCAATGCAGA CTGAAGAATT AGCTGGGAAT
    TCTCAGACAC TAAAAGAACC TGAAACAGTG GGAGCCCAAA GTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842192.1
    CDS437..6724
    Translation

    Target ORF information:

    RefSeq Version XM_024986424.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986424.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG ATTGTCGATA CAATAGAGAA
    GGAGAACAGG AGATGCCTGT TGCACAAGGT CATGATGAGG AGGAGGAGGA GGAGGAAGCA
    GAAGATGAGG CCCTCAGTGG AGCTTCACTG TCTAGTCACA GCAGCAGCCT GATCATTGAG
    ACTCCTGAAG ATGCTGAATT TGAACATAAG ATCCAGAAGC TTATGGCTGC AAAACTGAAA
    CTTAGACACT TACAGAACCT TGTAGCCATG GTGCAGGACG ATGATGCAGC CAATCAAGGA
    GTTACTTCTG TGGAGGATTT CTTCCCAGCA GAAGACACCA AACAAAATGC AAATAACACT
    AGAGGAAATG CCAATAAAAC ACAAAAAGAT GCTAGAGTAA ATGAAAAGGC AAGAGAGAAA
    TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAACTCA AACAATTGCA GGAAGAAAGA
    AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGAATTGC AAAAGGCATG TCCTAACCTA
    CAGCTGTCAG CTGCTAGTGT GGGTAATTGT CCCACAAAAA CACATATGCC AGCTGTTACT
    TCATCCCCAA CCGTTAATGA GAATGAAGCC AGTACAAACA AATCTGGTTT TGAGCCTCAT
    GATTCTTCAG TGGTAGATAA TGAGGTATGG TCAGATATGC GAAGACATGA AATGTTAAGG
    GAGGAGCTAC GACAGAGAAG AAAGCAGCTG GAAGCTCTGA TGGCTGAACA TCAGAGGAGG
    CAAGGTCTGG CTGAAACTGC ATCTCCAGTG GCTGTTTCAC TGAGAAGTGA TGGATCTGAG
    AACCTGTGTA CACCTCAGCA AAGCAGAACA GAAAAAACAA TGGCAACTTG GGGAGGTTCT
    ACCCAGTGTG CCCTAGATGA AGAAGGGGAT GAAGATGGTT ACCTTTCTGA AGGAATTATT
    CGGACAGACG AAGAGGAGGA AGAGGAGGAG CAAGATGCCA GTTCCAATGA TAACTTTCAT
    ATGTATCCTC CAAACAGTGC AAATCGTAAT TCTTACAATG TAAAGGAAAC TAAAAATAGG
    TGGAAGAACA ATCGCCCTTT TTCAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA
    CAACAGAATA TCAGCATGCA GCGTCAGGAA AACCTTCGCT GGGTGTCCGA ACTCTCTTAT
    GTAGAAGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TAAAGAAACA ACTTGATTTC
    AGTGTCAATA TTTGTCAGAC TTTAATGCAA GACCAGCAGA CTCTGTCTTG TCTGCTACAA
    ACTCTTCTCA CAAGTCCTTA TAGTGTTATG CCCAGCAGTG TTGCATCCCC GCAAGTGCAC
    CTTATAATGC ACCAGTTGAA CCAGTGCTAT ACCCAGCTAA CGTGGCAGCA GAATAATGTA
    CAGAGGTTGA AACAAATGCT CAGTGAACTC ATGCACCAAC AAAATCAGCA TCCAGAAAAA
    CCTGCAAGCA AGGAAGGAGG CAGTGGTGCA TCACACCCTC CTTCTCCCAG TTTATTTTGT
    CCTTTCAACT TTCCAACTCA GCCTGTAAAT CTGTTTAACC TACCGGGATT TACTAATTTT
    TCGTCATTTG CACCAAGTAT GAATTTCAGC CCTTTATTTC CTTCTAATTT TGGAGATTTT
    TCTCAAAATA TTTCTACACC CACTGAACAG CAGCAACCGT TAGCCCAGAA TCCTTCAGGG
    AAAACAGAAT ACATGGCTTT TCCAAAACCT TTTGAAAGTA GTTCTTCTAT TGGAGCAGAA
    AAACAAAGGA ATCAAAAACA GCCTGAAGAG GAGGTGGAGA ACAGTAGGAC ACCATGGTTT
    TATGACCAAG AAGGTGAGGT AGAAAAGCCA TTTCTCAAGA CTGGATTTGC AGTGTCTGTA
    GAGAAAACAA CAAATAGTAA TCGCAAAAAT CAACTAGATA CCAGTAGGAG AAGACGCCAG
    TTTGATGAAG TGTCCTTAGA AAGCTTTAAC AGTATGCTTG ACCCAGTAGA TCCAACAACA
    GTAACTAAAA CATTCAAGTC AAGAAAAGCA TCTGCACAGG CCAGCCTGGC ATCTAAAGAT
    AAAACTCCCA AATCAAAGAG TAAGAAGAGG CATTCTGCTC AGCCGAAAAG CAGAGTGAAA
    AATATTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGAAAC
    AGACATTCAG CCCAGACCGA AGAGCCTGTT CAAGCAAAAG TACTCAGCAG AAAGAAACAT
    GAGCAGCTGG AAAAAGTCAT AAGATGTAGT AGGTCTACAG AAATATCTTC AGAAACCAGT
    AGTGATTTTT CCATGTTCGA AGCTTTGCGG GATACCATTT ATTCTGAAGT AGCTACGTTG
    ATTTCTCAAA ATGAATCTCG TCCACATTTT CTTATTGAAC TCTTCCATGA GCTTCAGCTA
    CTTAATACAG ACTATTTGAG ACAGAGGGCC TTATATGCAT TGCAGGACAT AGTATCCAGG
    CATATTTCTG AGAACCATGA AAAAGAAGGA GAAAATATAA AGTCCGTGAA TTCTGGTACT
    TGGATAGCAT CCAACTCAGA ACTTACTCCC AGTGAAAGCC TTGCTACTAC TGATGATGAA
    ACTTTTGAGA AGAACTTTGA GAGAGAAACA CATAAAATAA GTGAGCAAAA TGATGCTGAT
    AATGCTAGTG TATCTTCAAC TTGTGAGCCT TTTGCAACAG ATGTTCTGGG TGACACTGTG
    ATTCACTTAG ATCAGGCATT AGCCAGAATG AGAGACTACA CGCATATGAA GATGGAGGCT
    GAAAGTAGTC CAGACGTCAG GTGCACCTGC AGGATCGTTG AGGATGACGA TGGTGCTGCT
    GCCACGACCA CGATTAGTAA TAATTTAGAA GAAACTCCTG TTATTGAAAA TCATAGTTCC
    CAGCAACCCG TGAGTGAAGT TGCTACCGTC CCGTGTCCTA GAATTGATAC TCAGCAACTG
    GACCGGCAAA TTAAAGCAAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT
    GAAGTATGTT CTTCTCAACT TCTAACCTCA GTAAGACGCA TGGTTTTGGT CCTTACTCAG
    CAAAACAATG AGAGCAAAGA GTTTGTAAAG TTCTTTCATA AGCAGCTTGG AAGTATATTA
    CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAGG ATTGTGGAGA AGATCTTCTT
    GTAGAGATAT CTGAAGTGTT ATTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG
    GATAATAATA GTGTAACTGT TAAACAGAGA TGCAAAAGGA AAATAGAAGC TGCTGGAGTG
    ATACAGTCTT ATGCAAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCACAG TTCACCTGCT
    GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAGACGG AAACAGTTAA GCAGACTCAA
    ACGTCTGAGG TTTATGATGG TGACGGCCCC AAAAATGTGA GATCTGATAT TTCTGATCAA
    GAGGATGATG AAGAAAGTGA AGAATGTCCA GTGTCTATTA GTTTGTCCAA AGCTGAAACT
    CAGGCTTTGA CTAATTACGG AAGTGGAGAA GATGAGAATG AAGATGAAGA GATAGAAGAA
    TTTGAAGAGG GACCTGTAGA TGTCCAGACG TCACTTCAGG CTAACACTGA AACTACAGAA
    GAAAATGAAC ACGATGATCA GGTTCCACAG CATGATTTTG AAAAATCATC AGAAAGCAAA
    AATGTCCCAT CAGAACAAGA ACCCACTACT AGTAAAGGTG ACCAGGATAG CACTCCTGTG
    AAGCCATGTT ACCTCAATAT CGTGGAAAAT GAGCAACCTT TAAATAGCCC TGTCCAGAAG
    GACACTCTCA CTACCATCGA CTCGTCCAGT CAACCTAACC CTTTGCCTTT ACCTTTAACT
    GAAATTGAAA CCTTGGTGCC TAGAGTCAAA GAAGTGAAAT CTGCTCAGGA GACTCCTGAA
    AGTTCTTTGG CTGGAAGTCC TGATACTGAA TCTCCAGTGT TAGTGAATGA CTATGAAGCA
    GAATCTGGTA ACATAAGTCA AAAATCTGAT GAAGAAGACT TTGTGAAAGT TGAAGATTTA
    CCACTTAAAC TGACAATATA TTCAGAGGCA GATCTAAGGA AGAAAATGGC CGAAGAAGAA
    CAGAAAAACC ACTTATCTGG TGAAATATGT GCAATGCAGA CTGAAGAATT AGCTGGGAAT
    TCTCAGACAC TAAAAGAACC TGAAACAGTG GGAGCCCAAA GTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231470
    Clone ID Related Accession (Same CDS sequence) XM_024986425.1
    Accession Version XM_024986425.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG ATTGTCGATA CAATAGAGAA
    GGAGAACAGG AGATGCCTGT TGCACAAGGT CATGATGAGG AGGAGGAGGA GGAGGAAGCA
    GAAGATGAGG CCCTCAGTGG AGCTTCACTG TCTAGTCACA GCAGCAGCCT GATCATTGAG
    ACTCCTGAAG ATGCTGAATT TGAACATAAG ATCCAGAAGC TTATGGCTGC AAAACTGAAA
    CTTAGACACT TACAGAACCT TGTAGCCATG GTGCAGGACG ATGATGCAGC CAATCAAGGA
    GTTACTTCTG TGGAGGATTT CTTCCCAGCA GAAGACACCA AACAAAATGC AAATAACACT
    AGAGGAAATG CCAATAAAAC ACAAAAAGAT GCTAGAGTAA ATGAAAAGGC AAGAGAGAAA
    TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAACTCA AACAATTGCA GGAAGAAAGA
    AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGAATTGC AAAAGGCATG TCCTAACCTA
    CAGCTGTCAG CTGCTAGTGT GGGTAATTGT CCCACAAAAA CACATATGCC AGCTGTTACT
    TCATCCCCAA CCGTTAATGA GAATGAAGCC AGTACAAACA AATCTGGTTT TGAGCCTCAT
    GATTCTTCAG TGGTAGATAA TGAGGTATGG TCAGATATGC GAAGACATGA AATGTTAAGG
    GAGGAGCTAC GACAGAGAAG AAAGCAGCTG GAAGCTCTGA TGGCTGAACA TCAGAGGAGG
    CAAGGTCTGG CTGAAACTGC ATCTCCAGTG GCTGTTTCAC TGAGAAGTGA TGGATCTGAG
    AACCTGTGTA CACCTCAGCA AAGCAGAACA GAAAAAACAA TGGCAACTTG GGGAGGTTCT
    ACCCAGTGTG CCCTAGATGA AGAAGGGGAT GAAGATGGTT ACCTTTCTGA AGGAATTATT
    CGGACAGACG AAGAGGAGGA AGAGGAGGAG CAAGATGCCA GTTCCAATGA TAACTTTCAT
    ATGTATCCTC CAAACAGTGC AAATCGTAAT TCTTACAATG TAAAGGAAAC TAAAAATAGG
    TGGAAGAACA ATCGCCCTTT TTCAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA
    CAACAGAATA TCAGCATGCA GCGTCAGGAA AACCTTCGCT GGGTGTCCGA ACTCTCTTAT
    GTAGAAGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TAAAGAAACA ACTTGATTTC
    AGTGTCAATA TTTGTCAGAC TTTAATGCAA GACCAGCAGA CTCTGTCTTG TCTGCTACAA
    ACTCTTCTCA CAAGTCCTTA TAGTGTTATG CCCAGCAGTG TTGCATCCCC GCAAGTGCAC
    CTTATAATGC ACCAGTTGAA CCAGTGCTAT ACCCAGCTAA CGTGGCAGCA GAATAATGTA
    CAGAGGTTGA AACAAATGCT CAGTGAACTC ATGCACCAAC AAAATCAGCA TCCAGAAAAA
    CCTGCAAGCA AGGAAGGAGG CAGTGGTGCA TCACACCCTC CTTCTCCCAG TTTATTTTGT
    CCTTTCAACT TTCCAACTCA GCCTGTAAAT CTGTTTAACC TACCGGGATT TACTAATTTT
    TCGTCATTTG CACCAAGTAT GAATTTCAGC CCTTTATTTC CTTCTAATTT TGGAGATTTT
    TCTCAAAATA TTTCTACACC CACTGAACAG CAGCAACCGT TAGCCCAGAA TCCTTCAGGG
    AAAACAGAAT ACATGGCTTT TCCAAAACCT TTTGAAAGTA GTTCTTCTAT TGGAGCAGAA
    AAACAAAGGA ATCAAAAACA GCCTGAAGAG GAGGTGGAGA ACAGTAGGAC ACCATGGTTT
    TATGACCAAG AAGGTGAGGT AGAAAAGCCA TTTCTCAAGA CTGGATTTGC AGTGTCTGTA
    GAGAAAACAA CAAATAGTAA TCGCAAAAAT CAACTAGATA CCAGTAGGAG AAGACGCCAG
    TTTGATGAAG TGTCCTTAGA AAGCTTTAAC AGTATGCTTG ACCCAGTAGA TCCAACAACA
    GTAACTAAAA CATTCAAGTC AAGAAAAGCA TCTGCACAGG CCAGCCTGGC ATCTAAAGAT
    AAAACTCCCA AATCAAAGAG TAAGAAGAGG CATTCTGCTC AGCCGAAAAG CAGAGTGAAA
    AATATTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGAAAC
    AGACATTCAG CCCAGACCGA AGAGCCTGTT CAAGCAAAAG TACTCAGCAG AAAGAAACAT
    GAGCAGCTGG AAAAAGTCAT AAGATGTAGT AGGTCTACAG AAATATCTTC AGCACATGCT
    AGGAGAATAC TACAGCAGTC TAACAGAAAT GCATGCAATG AAGCGCCAGA AACCAGTAGT
    GATTTTTCCA TGTTCGAAGC TTTGCGGGAT ACCATTTATT CTGAAGTAGC TACGTTGATT
    TCTCAAAATG AATCTCGTCC ACATTTTCTT ATTGAACTCT TCCATGAGCT TCAGCTACTT
    AATACAGACT ATTTGAGACA GAGGGCCTTA TATGCATTGC AGGACATAGT ATCCAGGCAT
    ATTTCTGAGA ACCATGAAAA AGAAGGAGAA AATATAAAGT CCGTGAATTC TGGTACTTGG
    ATAGCATCCA ACTCAGAACT TACTCCCAGT GAAAGCCTTG CTACTACTGA TGATGAAACT
    TTTGAGAAGA ACTTTGAGAG AGAAACACAT AAAATAAGTG AGCAAAATGA TGCTGATAAT
    GCTAGTGTAT CTTCAACTTG TGAGCCTTTT GCAACAGATG TTCTGGGTGA CACTGTGATT
    CACTTAGATC AGGCATTAGC CAGAATGAGA GACTACACGC ATATGAAGAT GGAGGCTGAA
    AGTAGTCCAG ACGTCAGGTG CACCTGCAGG ATCGTTGAGG ATGACGATGG TGCTGCTGCC
    ACGACCACGA TTAGTAATAA TTTAGAAGAA ACTCCTGTTA TTGAAAATCA TAGTTCCCAG
    CAACCCGTGA GTGAAGTTGC TACCGTCCCG TGTCCTAGAA TTGATACTCA GCAACTGGAC
    CGGCAAATTA AAGCAATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGAGCA CATGGATGAA
    GTATGTTCTT CTCAACTTCT AACCTCAGTA AGACGCATGG TTTTGGTCCT TACTCAGCAA
    AACAATGAGA GCAAAGAGTT TGTAAAGTTC TTTCATAAGC AGCTTGGAAG TATATTACAG
    GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAGGATT GTGGAGAAGA TCTTCTTGTA
    GAGATATCTG AAGTGTTATT TAATGAATTG GCTTTCTTTA AGCTCATGCA AGATTTGGAT
    AATAATAGTG TAACTGTTAA ACAGAGATGC AAAAGGAAAA TAGAAGCTGC TGGAGTGATA
    CAGTCTTATG CAAAAGAGGA CAAGGATGAG ACGGAAACAG TTAAGCAGAC TCAAACGTCT
    GAGGTTTATG ATGGTGACGG CCCCAAAAAT GTGAGATCTG ATATTTCTGA TCAAGAGGAT
    GATGAAGAAA GTGAAGAATG TCCAGTGTCT ATTAGTTTGT CCAAAGCTGA AACTCAGGCT
    TTGACTAATT ACGGAAGTGG AGAAGATGAG AATGAAGATG AAGAGATAGA AGAATTTGAA
    GAGGGACCTG TAGATGTCCA GACGTCACTT CAGGCTAACA CTGAAACTAC AGAAGAAAAT
    GAACACGATG ATCAGGTTCC ACAGCATGAT TTTGAAAAAT CATCAGAAAG CAAAAATGTC
    CCATCAGAAC AAGAACCCAC TACTAGTAAA GGTGACCAGG ATAGCACTCC TGTGAAGCCA
    TGTTACCTCA ATATCGTGGA AAATGAGCAA CCTTTAAATA GCCCTGTCCA GAAGGACACT
    CTCACTACCA TCGACTCGTC CAGTCAACCT AACCCTTTGC CTTTACCTTT AACTGAAATT
    GAAACCTTGG TGCCTAGAGT CAAAGAAGTG AAATCTGCTC AGGAGACTCC TGAAAGTTCT
    TTGGCTGGAA GTCCTGATAC TGAATCTCCA GTGTTAGTGA ATGACTATGA AGCAGAATCT
    GGTAACATAA GTCAAAAATC TGATGAAGAA GACTTTGTGA AAGTTGAAGA TTTACCACTT
    AAACTGACAA TATATTCAGA GGCAGATCTA AGGAAGAAAA TGGCCGAAGA AGAACAGAAA
    AACCACTTAT CTGGTGAAAT ATGTGCAATG CAGACTGAAG AATTAGCTGG GAATTCTCAG
    ACACTAAAAG AACCTGAAAC AGTGGGAGCC CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842193.1
    CDS441..6722
    Translation

    Target ORF information:

    RefSeq Version XM_024986425.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986425.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG ATTGTCGATA CAATAGAGAA
    GGAGAACAGG AGATGCCTGT TGCACAAGGT CATGATGAGG AGGAGGAGGA GGAGGAAGCA
    GAAGATGAGG CCCTCAGTGG AGCTTCACTG TCTAGTCACA GCAGCAGCCT GATCATTGAG
    ACTCCTGAAG ATGCTGAATT TGAACATAAG ATCCAGAAGC TTATGGCTGC AAAACTGAAA
    CTTAGACACT TACAGAACCT TGTAGCCATG GTGCAGGACG ATGATGCAGC CAATCAAGGA
    GTTACTTCTG TGGAGGATTT CTTCCCAGCA GAAGACACCA AACAAAATGC AAATAACACT
    AGAGGAAATG CCAATAAAAC ACAAAAAGAT GCTAGAGTAA ATGAAAAGGC AAGAGAGAAA
    TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAACTCA AACAATTGCA GGAAGAAAGA
    AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGAATTGC AAAAGGCATG TCCTAACCTA
    CAGCTGTCAG CTGCTAGTGT GGGTAATTGT CCCACAAAAA CACATATGCC AGCTGTTACT
    TCATCCCCAA CCGTTAATGA GAATGAAGCC AGTACAAACA AATCTGGTTT TGAGCCTCAT
    GATTCTTCAG TGGTAGATAA TGAGGTATGG TCAGATATGC GAAGACATGA AATGTTAAGG
    GAGGAGCTAC GACAGAGAAG AAAGCAGCTG GAAGCTCTGA TGGCTGAACA TCAGAGGAGG
    CAAGGTCTGG CTGAAACTGC ATCTCCAGTG GCTGTTTCAC TGAGAAGTGA TGGATCTGAG
    AACCTGTGTA CACCTCAGCA AAGCAGAACA GAAAAAACAA TGGCAACTTG GGGAGGTTCT
    ACCCAGTGTG CCCTAGATGA AGAAGGGGAT GAAGATGGTT ACCTTTCTGA AGGAATTATT
    CGGACAGACG AAGAGGAGGA AGAGGAGGAG CAAGATGCCA GTTCCAATGA TAACTTTCAT
    ATGTATCCTC CAAACAGTGC AAATCGTAAT TCTTACAATG TAAAGGAAAC TAAAAATAGG
    TGGAAGAACA ATCGCCCTTT TTCAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA
    CAACAGAATA TCAGCATGCA GCGTCAGGAA AACCTTCGCT GGGTGTCCGA ACTCTCTTAT
    GTAGAAGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TAAAGAAACA ACTTGATTTC
    AGTGTCAATA TTTGTCAGAC TTTAATGCAA GACCAGCAGA CTCTGTCTTG TCTGCTACAA
    ACTCTTCTCA CAAGTCCTTA TAGTGTTATG CCCAGCAGTG TTGCATCCCC GCAAGTGCAC
    CTTATAATGC ACCAGTTGAA CCAGTGCTAT ACCCAGCTAA CGTGGCAGCA GAATAATGTA
    CAGAGGTTGA AACAAATGCT CAGTGAACTC ATGCACCAAC AAAATCAGCA TCCAGAAAAA
    CCTGCAAGCA AGGAAGGAGG CAGTGGTGCA TCACACCCTC CTTCTCCCAG TTTATTTTGT
    CCTTTCAACT TTCCAACTCA GCCTGTAAAT CTGTTTAACC TACCGGGATT TACTAATTTT
    TCGTCATTTG CACCAAGTAT GAATTTCAGC CCTTTATTTC CTTCTAATTT TGGAGATTTT
    TCTCAAAATA TTTCTACACC CACTGAACAG CAGCAACCGT TAGCCCAGAA TCCTTCAGGG
    AAAACAGAAT ACATGGCTTT TCCAAAACCT TTTGAAAGTA GTTCTTCTAT TGGAGCAGAA
    AAACAAAGGA ATCAAAAACA GCCTGAAGAG GAGGTGGAGA ACAGTAGGAC ACCATGGTTT
    TATGACCAAG AAGGTGAGGT AGAAAAGCCA TTTCTCAAGA CTGGATTTGC AGTGTCTGTA
    GAGAAAACAA CAAATAGTAA TCGCAAAAAT CAACTAGATA CCAGTAGGAG AAGACGCCAG
    TTTGATGAAG TGTCCTTAGA AAGCTTTAAC AGTATGCTTG ACCCAGTAGA TCCAACAACA
    GTAACTAAAA CATTCAAGTC AAGAAAAGCA TCTGCACAGG CCAGCCTGGC ATCTAAAGAT
    AAAACTCCCA AATCAAAGAG TAAGAAGAGG CATTCTGCTC AGCCGAAAAG CAGAGTGAAA
    AATATTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGAAAC
    AGACATTCAG CCCAGACCGA AGAGCCTGTT CAAGCAAAAG TACTCAGCAG AAAGAAACAT
    GAGCAGCTGG AAAAAGTCAT AAGATGTAGT AGGTCTACAG AAATATCTTC AGCACATGCT
    AGGAGAATAC TACAGCAGTC TAACAGAAAT GCATGCAATG AAGCGCCAGA AACCAGTAGT
    GATTTTTCCA TGTTCGAAGC TTTGCGGGAT ACCATTTATT CTGAAGTAGC TACGTTGATT
    TCTCAAAATG AATCTCGTCC ACATTTTCTT ATTGAACTCT TCCATGAGCT TCAGCTACTT
    AATACAGACT ATTTGAGACA GAGGGCCTTA TATGCATTGC AGGACATAGT ATCCAGGCAT
    ATTTCTGAGA ACCATGAAAA AGAAGGAGAA AATATAAAGT CCGTGAATTC TGGTACTTGG
    ATAGCATCCA ACTCAGAACT TACTCCCAGT GAAAGCCTTG CTACTACTGA TGATGAAACT
    TTTGAGAAGA ACTTTGAGAG AGAAACACAT AAAATAAGTG AGCAAAATGA TGCTGATAAT
    GCTAGTGTAT CTTCAACTTG TGAGCCTTTT GCAACAGATG TTCTGGGTGA CACTGTGATT
    CACTTAGATC AGGCATTAGC CAGAATGAGA GACTACACGC ATATGAAGAT GGAGGCTGAA
    AGTAGTCCAG ACGTCAGGTG CACCTGCAGG ATCGTTGAGG ATGACGATGG TGCTGCTGCC
    ACGACCACGA TTAGTAATAA TTTAGAAGAA ACTCCTGTTA TTGAAAATCA TAGTTCCCAG
    CAACCCGTGA GTGAAGTTGC TACCGTCCCG TGTCCTAGAA TTGATACTCA GCAACTGGAC
    CGGCAAATTA AAGCAATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGAGCA CATGGATGAA
    GTATGTTCTT CTCAACTTCT AACCTCAGTA AGACGCATGG TTTTGGTCCT TACTCAGCAA
    AACAATGAGA GCAAAGAGTT TGTAAAGTTC TTTCATAAGC AGCTTGGAAG TATATTACAG
    GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAGGATT GTGGAGAAGA TCTTCTTGTA
    GAGATATCTG AAGTGTTATT TAATGAATTG GCTTTCTTTA AGCTCATGCA AGATTTGGAT
    AATAATAGTG TAACTGTTAA ACAGAGATGC AAAAGGAAAA TAGAAGCTGC TGGAGTGATA
    CAGTCTTATG CAAAAGAGGA CAAGGATGAG ACGGAAACAG TTAAGCAGAC TCAAACGTCT
    GAGGTTTATG ATGGTGACGG CCCCAAAAAT GTGAGATCTG ATATTTCTGA TCAAGAGGAT
    GATGAAGAAA GTGAAGAATG TCCAGTGTCT ATTAGTTTGT CCAAAGCTGA AACTCAGGCT
    TTGACTAATT ACGGAAGTGG AGAAGATGAG AATGAAGATG AAGAGATAGA AGAATTTGAA
    GAGGGACCTG TAGATGTCCA GACGTCACTT CAGGCTAACA CTGAAACTAC AGAAGAAAAT
    GAACACGATG ATCAGGTTCC ACAGCATGAT TTTGAAAAAT CATCAGAAAG CAAAAATGTC
    CCATCAGAAC AAGAACCCAC TACTAGTAAA GGTGACCAGG ATAGCACTCC TGTGAAGCCA
    TGTTACCTCA ATATCGTGGA AAATGAGCAA CCTTTAAATA GCCCTGTCCA GAAGGACACT
    CTCACTACCA TCGACTCGTC CAGTCAACCT AACCCTTTGC CTTTACCTTT AACTGAAATT
    GAAACCTTGG TGCCTAGAGT CAAAGAAGTG AAATCTGCTC AGGAGACTCC TGAAAGTTCT
    TTGGCTGGAA GTCCTGATAC TGAATCTCCA GTGTTAGTGA ATGACTATGA AGCAGAATCT
    GGTAACATAA GTCAAAAATC TGATGAAGAA GACTTTGTGA AAGTTGAAGA TTTACCACTT
    AAACTGACAA TATATTCAGA GGCAGATCTA AGGAAGAAAA TGGCCGAAGA AGAACAGAAA
    AACCACTTAT CTGGTGAAAT ATGTGCAATG CAGACTGAAG AATTAGCTGG GAATTCTCAG
    ACACTAAAAG AACCTGAAAC AGTGGGAGCC CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231471
    Clone ID Related Accession (Same CDS sequence) XM_024986426.1
    Accession Version XM_024986426.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG ATTGTCGATA CAATAGAGAA
    GGAGAACAGG AGATGCCTGT TGCACAAGGT CATGATGAGG AGGAGGAGGA GGAGGAAGCA
    GAAGATGAGG CCCTCAGTGG AGCTTCACTG TCTAGTCACA GCAGCAGCCT GATCATTGAG
    ACTCCTGAAG ATGCTGAATT TGAACATAAG ATCCAGAAGC TTATGGCTGC AAAACTGAAA
    CTTAGACACT TACAGAACCT TGTAGCCATG GTGCAGGACG ATGATGCAGC CAATCAAGGA
    GTTACTTCTG TGGAGGATTT CTTCCCAGCA GAAGACACCA AACAAAATGC AAATAACACT
    AGAGGAAATG CCAATAAAAC ACAAAAAGAT GCTAGAGTAA ATGAAAAGGC AAGAGAGAAA
    TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAACTCA AACAATTGCA GGAAGAAAGA
    AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGAATTGC AAAAGGCATG TCCTAACCTA
    CAGCTGTCAG CTGCTAGTGT GGGTAATTGT CCCACAAAAA CACATATGCC AGCTGTTACT
    TCATCCCCAA CCGTTAATGA GAATGAAGCC AGTACAAACA AATCTGGTTT TGAGCCTCAT
    GATTCTTCAG TGGTAGATAA TGAGGTATGG TCAGATATGC GAAGACATGA AATGTTAAGG
    GAGGAGCTAC GACAGAGAAG AAAGCAGCTG GAAGCTCTGA TGGCTGAACA TCAGAGGAGG
    CAAGGTCTGG CTGAAACTGC ATCTCCAGTG GCTGTTTCAC TGAGAAGTGA TGGATCTGAG
    AACCTGTGTA CACCTCAGCA AAGCAGAACA GAAAAAACAA TGGCAACTTG GGGAGGTTCT
    ACCCAGTGTG CCCTAGATGA AGAAGGGGAT GAAGATGGTT ACCTTTCTGA AGGAATTATT
    CGGACAGACG AAGAGGAGGA AGAGGAGGAG CAAGATGCCA GTTCCAATGA TAACTTTCAT
    ATGTATCCTC CAAACAGTGC AAATCGTAAT TCTTACAATG TAAAGGAAAC TAAAAATAGG
    TGGAAGAACA ATCGCCCTTT TTCAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA
    CAACAGAATA TCAGCATGCA GCGTCAGGAA AACCTTCGCT GGGTGTCCGA ACTCTCTTAT
    GTAGAAGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TAAAGAAACA ACTTGATTTC
    AGTGTCAATA TTTGTCAGAC TTTAATGCAA GACCAGCAGA CTCTGTCTTG TCTGCTACAA
    ACTCTTCTCA CAAGTCCTTA TAGTGTTATG CCCAGCAGTG TTGCATCCCC GCAAGTGCAC
    CTTATAATGC ACCAGTTGAA CCAGTGCTAT ACCCAGCTAA CGTGGCAGCA GAATAATGTA
    CAGAGGTTGA AACAAATGCT CAGTGAACTC ATGCACCAAC AAAATCAGCA TCCAGAAAAA
    CCTGCAAGCA AGGAAGGAGG CAGTGGTGCA TCACACCCTC CTTCTCCCAG TTTATTTTGT
    CCTTTCAACT TTCCAACTCA GCCTGTAAAT CTGTTTAACC TACCGGGATT TACTAATTTT
    TCGTCATTTG CACCAAGTAT GAATTTCAGC CCTTTATTTC CTTCTAATTT TGGAGATTTT
    TCTCAAAATA TTTCTACACC CACTGAACAG CAGCAACCGT TAGCCCAGAA TCCTTCAGGG
    AAAACAGAAT ACATGGCTTT TCCAAAACCT TTTGAAAGTA GTTCTTCTAT TGGAGCAGAA
    AAACAAAGGA ATCAAAAACA GCCTGAAGAG GAGGTGGAGA ACAGTAGGAC ACCATGGTTT
    TATGACCAAG AAGGTGAGGT AGAAAAGCCA TTTCTCAAGA CTGGATTTGC AGTGTCTGTA
    GAGAAAACAA CAAATAGTAA TCGCAAAAAT CAACTAGATA CCAGTAGGAG AAGACGCCAG
    TTTGATGAAG TGTCCTTAGA AAGCTTTAAC AGTATGCTTG ACCCAGTAGA TCCAACAACA
    GTAACTAAAA CATTCAAGTC AAGAAAAGCA TCTGCACAGG CCAGCCTGGC ATCTAAAGAT
    AAAACTCCCA AATCAAAGAG TAAGAAGAGG CATTCTGCTC AGCCGAAAAG CAGAGTGAAA
    AATATTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGAAAC
    AGACATTCAG CCCAGACCGA AGAGCCTGTT CAAGCAAAAG TACTCAGCAG AAAGAAACAT
    GAGCAGCTGG AAAAAGTCAT AAGATGTAGT AGGTCTACAG AAATATCTTC AGAAACCAGT
    AGTGATTTTT CCATGTTCGA AGCTTTGCGG GATACCATTT ATTCTGAAGT AGCTACGTTG
    ATTTCTCAAA ATGAATCTCG TCCACATTTT CTTATTGAAC TCTTCCATGA GCTTCAGCTA
    CTTAATACAG ACTATTTGAG ACAGAGGGCC TTATATGCAT TGCAGGACAT AGTATCCAGG
    CATATTTCTG AGAACCATGA AAAAGAAGGA GAAAATATAA AGTCCGTGAA TTCTGGTACT
    TGGATAGCAT CCAACTCAGA ACTTACTCCC AGTGAAAGCC TTGCTACTAC TGATGATGAA
    ACTTTTGAGA AGAACTTTGA GAGAGAAACA CATAAAATAA GTGAGCAAAA TGATGCTGAT
    AATGCTAGTG TATCTTCAAC TTGTGAGCCT TTTGCAACAG ATGTTCTGGG TGACACTGTG
    ATTCACTTAG ATCAGGCATT AGCCAGAATG AGAGACTACA CGCATATGAA GATGGAGGCT
    GAAAGTAGTC CAGACGTCAG GTGCACCTGC AGGATCGTTG AGGATGACGA TGGTGCTGCT
    GCCACGACCA CGATTAGTAA TAATTTAGAA GAAACTCCTG TTATTGAAAA TCATAGTTCC
    CAGCAACCCG TGAGTGAAGT TGCTACCGTC CCGTGTCCTA GAATTGATAC TCAGCAACTG
    GACCGGCAAA TTAAAGCAAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT
    GAAGTATGTT CTTCTCAACT TCTAACCTCA GTAAGACGCA TGGTTTTGGT CCTTACTCAG
    CAAAACAATG AGAGCAAAGA GTTTGTAAAG TTCTTTCATA AGCAGCTTGG AAGTATATTA
    CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAGG ATTGTGGAGA AGATCTTCTT
    GTAGAGATAT CTGAAGTGTT ATTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG
    GATAATAATA GTGTAACTGT TAAACAGAGA TGCAAAAGGA AAATAGAAGC TGCTGGAGTG
    ATACAGTCTT ATGCAAAAGA GGACAAGGAT GAGACGGAAA CAGTTAAGCA GACTCAAACG
    TCTGAGGTTT ATGATGGTGA CGGCCCCAAA AATGTGAGAT CTGATATTTC TGATCAAGAG
    GATGATGAAG AAAGTGAAGA ATGTCCAGTG TCTATTAGTT TGTCCAAAGC TGAAACTCAG
    GCTTTGACTA ATTACGGAAG TGGAGAAGAT GAGAATGAAG ATGAAGAGAT AGAAGAATTT
    GAAGAGGGAC CTGTAGATGT CCAGACGTCA CTTCAGGCTA ACACTGAAAC TACAGAAGAA
    AATGAACACG ATGATCAGGT TCCACAGCAT GATTTTGAAA AATCATCAGA AAGCAAAAAT
    GTCCCATCAG AACAAGAACC CACTACTAGT AAAGGTGACC AGGATAGCAC TCCTGTGAAG
    CCATGTTACC TCAATATCGT GGAAAATGAG CAACCTTTAA ATAGCCCTGT CCAGAAGGAC
    ACTCTCACTA CCATCGACTC GTCCAGTCAA CCTAACCCTT TGCCTTTACC TTTAACTGAA
    ATTGAAACCT TGGTGCCTAG AGTCAAAGAA GTGAAATCTG CTCAGGAGAC TCCTGAAAGT
    TCTTTGGCTG GAAGTCCTGA TACTGAATCT CCAGTGTTAG TGAATGACTA TGAAGCAGAA
    TCTGGTAACA TAAGTCAAAA ATCTGATGAA GAAGACTTTG TGAAAGTTGA AGATTTACCA
    CTTAAACTGA CAATATATTC AGAGGCAGAT CTAAGGAAGA AAATGGCCGA AGAAGAACAG
    AAAAACCACT TATCTGGTGA AATATGTGCA ATGCAGACTG AAGAATTAGC TGGGAATTCT
    CAGACACTAA AAGAACCTGA AACAGTGGGA GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842194.1
    CDS455..6679
    Translation

    Target ORF information:

    RefSeq Version XM_024986426.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986426.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG ATTGTCGATA CAATAGAGAA
    GGAGAACAGG AGATGCCTGT TGCACAAGGT CATGATGAGG AGGAGGAGGA GGAGGAAGCA
    GAAGATGAGG CCCTCAGTGG AGCTTCACTG TCTAGTCACA GCAGCAGCCT GATCATTGAG
    ACTCCTGAAG ATGCTGAATT TGAACATAAG ATCCAGAAGC TTATGGCTGC AAAACTGAAA
    CTTAGACACT TACAGAACCT TGTAGCCATG GTGCAGGACG ATGATGCAGC CAATCAAGGA
    GTTACTTCTG TGGAGGATTT CTTCCCAGCA GAAGACACCA AACAAAATGC AAATAACACT
    AGAGGAAATG CCAATAAAAC ACAAAAAGAT GCTAGAGTAA ATGAAAAGGC AAGAGAGAAA
    TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAACTCA AACAATTGCA GGAAGAAAGA
    AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGAATTGC AAAAGGCATG TCCTAACCTA
    CAGCTGTCAG CTGCTAGTGT GGGTAATTGT CCCACAAAAA CACATATGCC AGCTGTTACT
    TCATCCCCAA CCGTTAATGA GAATGAAGCC AGTACAAACA AATCTGGTTT TGAGCCTCAT
    GATTCTTCAG TGGTAGATAA TGAGGTATGG TCAGATATGC GAAGACATGA AATGTTAAGG
    GAGGAGCTAC GACAGAGAAG AAAGCAGCTG GAAGCTCTGA TGGCTGAACA TCAGAGGAGG
    CAAGGTCTGG CTGAAACTGC ATCTCCAGTG GCTGTTTCAC TGAGAAGTGA TGGATCTGAG
    AACCTGTGTA CACCTCAGCA AAGCAGAACA GAAAAAACAA TGGCAACTTG GGGAGGTTCT
    ACCCAGTGTG CCCTAGATGA AGAAGGGGAT GAAGATGGTT ACCTTTCTGA AGGAATTATT
    CGGACAGACG AAGAGGAGGA AGAGGAGGAG CAAGATGCCA GTTCCAATGA TAACTTTCAT
    ATGTATCCTC CAAACAGTGC AAATCGTAAT TCTTACAATG TAAAGGAAAC TAAAAATAGG
    TGGAAGAACA ATCGCCCTTT TTCAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA
    CAACAGAATA TCAGCATGCA GCGTCAGGAA AACCTTCGCT GGGTGTCCGA ACTCTCTTAT
    GTAGAAGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TAAAGAAACA ACTTGATTTC
    AGTGTCAATA TTTGTCAGAC TTTAATGCAA GACCAGCAGA CTCTGTCTTG TCTGCTACAA
    ACTCTTCTCA CAAGTCCTTA TAGTGTTATG CCCAGCAGTG TTGCATCCCC GCAAGTGCAC
    CTTATAATGC ACCAGTTGAA CCAGTGCTAT ACCCAGCTAA CGTGGCAGCA GAATAATGTA
    CAGAGGTTGA AACAAATGCT CAGTGAACTC ATGCACCAAC AAAATCAGCA TCCAGAAAAA
    CCTGCAAGCA AGGAAGGAGG CAGTGGTGCA TCACACCCTC CTTCTCCCAG TTTATTTTGT
    CCTTTCAACT TTCCAACTCA GCCTGTAAAT CTGTTTAACC TACCGGGATT TACTAATTTT
    TCGTCATTTG CACCAAGTAT GAATTTCAGC CCTTTATTTC CTTCTAATTT TGGAGATTTT
    TCTCAAAATA TTTCTACACC CACTGAACAG CAGCAACCGT TAGCCCAGAA TCCTTCAGGG
    AAAACAGAAT ACATGGCTTT TCCAAAACCT TTTGAAAGTA GTTCTTCTAT TGGAGCAGAA
    AAACAAAGGA ATCAAAAACA GCCTGAAGAG GAGGTGGAGA ACAGTAGGAC ACCATGGTTT
    TATGACCAAG AAGGTGAGGT AGAAAAGCCA TTTCTCAAGA CTGGATTTGC AGTGTCTGTA
    GAGAAAACAA CAAATAGTAA TCGCAAAAAT CAACTAGATA CCAGTAGGAG AAGACGCCAG
    TTTGATGAAG TGTCCTTAGA AAGCTTTAAC AGTATGCTTG ACCCAGTAGA TCCAACAACA
    GTAACTAAAA CATTCAAGTC AAGAAAAGCA TCTGCACAGG CCAGCCTGGC ATCTAAAGAT
    AAAACTCCCA AATCAAAGAG TAAGAAGAGG CATTCTGCTC AGCCGAAAAG CAGAGTGAAA
    AATATTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGAAAC
    AGACATTCAG CCCAGACCGA AGAGCCTGTT CAAGCAAAAG TACTCAGCAG AAAGAAACAT
    GAGCAGCTGG AAAAAGTCAT AAGATGTAGT AGGTCTACAG AAATATCTTC AGAAACCAGT
    AGTGATTTTT CCATGTTCGA AGCTTTGCGG GATACCATTT ATTCTGAAGT AGCTACGTTG
    ATTTCTCAAA ATGAATCTCG TCCACATTTT CTTATTGAAC TCTTCCATGA GCTTCAGCTA
    CTTAATACAG ACTATTTGAG ACAGAGGGCC TTATATGCAT TGCAGGACAT AGTATCCAGG
    CATATTTCTG AGAACCATGA AAAAGAAGGA GAAAATATAA AGTCCGTGAA TTCTGGTACT
    TGGATAGCAT CCAACTCAGA ACTTACTCCC AGTGAAAGCC TTGCTACTAC TGATGATGAA
    ACTTTTGAGA AGAACTTTGA GAGAGAAACA CATAAAATAA GTGAGCAAAA TGATGCTGAT
    AATGCTAGTG TATCTTCAAC TTGTGAGCCT TTTGCAACAG ATGTTCTGGG TGACACTGTG
    ATTCACTTAG ATCAGGCATT AGCCAGAATG AGAGACTACA CGCATATGAA GATGGAGGCT
    GAAAGTAGTC CAGACGTCAG GTGCACCTGC AGGATCGTTG AGGATGACGA TGGTGCTGCT
    GCCACGACCA CGATTAGTAA TAATTTAGAA GAAACTCCTG TTATTGAAAA TCATAGTTCC
    CAGCAACCCG TGAGTGAAGT TGCTACCGTC CCGTGTCCTA GAATTGATAC TCAGCAACTG
    GACCGGCAAA TTAAAGCAAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT
    GAAGTATGTT CTTCTCAACT TCTAACCTCA GTAAGACGCA TGGTTTTGGT CCTTACTCAG
    CAAAACAATG AGAGCAAAGA GTTTGTAAAG TTCTTTCATA AGCAGCTTGG AAGTATATTA
    CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAGG ATTGTGGAGA AGATCTTCTT
    GTAGAGATAT CTGAAGTGTT ATTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG
    GATAATAATA GTGTAACTGT TAAACAGAGA TGCAAAAGGA AAATAGAAGC TGCTGGAGTG
    ATACAGTCTT ATGCAAAAGA GGACAAGGAT GAGACGGAAA CAGTTAAGCA GACTCAAACG
    TCTGAGGTTT ATGATGGTGA CGGCCCCAAA AATGTGAGAT CTGATATTTC TGATCAAGAG
    GATGATGAAG AAAGTGAAGA ATGTCCAGTG TCTATTAGTT TGTCCAAAGC TGAAACTCAG
    GCTTTGACTA ATTACGGAAG TGGAGAAGAT GAGAATGAAG ATGAAGAGAT AGAAGAATTT
    GAAGAGGGAC CTGTAGATGT CCAGACGTCA CTTCAGGCTA ACACTGAAAC TACAGAAGAA
    AATGAACACG ATGATCAGGT TCCACAGCAT GATTTTGAAA AATCATCAGA AAGCAAAAAT
    GTCCCATCAG AACAAGAACC CACTACTAGT AAAGGTGACC AGGATAGCAC TCCTGTGAAG
    CCATGTTACC TCAATATCGT GGAAAATGAG CAACCTTTAA ATAGCCCTGT CCAGAAGGAC
    ACTCTCACTA CCATCGACTC GTCCAGTCAA CCTAACCCTT TGCCTTTACC TTTAACTGAA
    ATTGAAACCT TGGTGCCTAG AGTCAAAGAA GTGAAATCTG CTCAGGAGAC TCCTGAAAGT
    TCTTTGGCTG GAAGTCCTGA TACTGAATCT CCAGTGTTAG TGAATGACTA TGAAGCAGAA
    TCTGGTAACA TAAGTCAAAA ATCTGATGAA GAAGACTTTG TGAAAGTTGA AGATTTACCA
    CTTAAACTGA CAATATATTC AGAGGCAGAT CTAAGGAAGA AAATGGCCGA AGAAGAACAG
    AAAAACCACT TATCTGGTGA AATATGTGCA ATGCAGACTG AAGAATTAGC TGGGAATTCT
    CAGACACTAA AAGAACCTGA AACAGTGGGA GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231472
    Clone ID Related Accession (Same CDS sequence) XM_024986427.1
    Accession Version XM_024986427.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG CAGATTGTCG ATACAATAGA
    GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGATG AGGAGGAGGA GGAGGAGGAA
    GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT
    GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG
    AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA
    GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC
    ACTAGAGGAA ATGCCAATAA AACACAAAAA GATGCTAGAG TAAATGAAAA GGCAAGAGAG
    AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA
    AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC
    CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT
    ACTTCATCCC CAACCGTTAA TGAGAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT
    CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA
    AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG
    AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT
    GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT
    TCTACCCAGT GTGCCCTAGA TGAAGAAGGG GATGAAGATG GTTACCTTTC TGAAGGAATT
    ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT
    CATATGTATC CTCCAAACAG TGCAAATCGT AATTCTTACA ATGTAAAGGA AACTAAAAAT
    AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA
    AGACAACAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT
    TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT
    TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA
    CAAACTCTTC TCACAAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG
    CACCTTATAA TGCACCAGTT GAACCAGTGC TATACCCAGC TAACGTGGCA GCAGAATAAT
    GTACAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCACC AACAAAATCA GCATCCAGAA
    AAACCTGCAA GCAAGGAAGG AGGCAGTGGT GCATCACACC CTCCTTCTCC CAGTTTATTT
    TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG ATTTACTAAT
    TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT
    TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA
    GGGAAAACAG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCA
    GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG
    TTTTATGACC AAGAAGGTGA GGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT
    GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC
    CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA
    ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA
    GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTG CTCAGCCGAA AAGCAGAGTG
    AAAAATATTG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA
    AACAGACATT CAGCCCAGAC CGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAA
    CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGCACAT
    GCTAGGAGAA TACTACAGCA GTCTAACAGA AATGCATGCA ATGAAGCGCC AGAAACCAGT
    AGTGATTTTT CCATGTTCGA AGCTTTGCGG GATACCATTT ATTCTGAAGT AGCTACGTTG
    ATTTCTCAAA ATGAATCTCG TCCACATTTT CTTATTGAAC TCTTCCATGA GCTTCAGCTA
    CTTAATACAG ACTATTTGAG ACAGAGGGCC TTATATGCAT TGCAGGACAT AGTATCCAGG
    CATATTTCTG AGAACCATGA AAAAGAAGGA GAAAATATAA AGTCCGTGAA TTCTGGTACT
    TGGATAGCAT CCAACTCAGA ACTTACTCCC AGTGAAAGCC TTGCTACTAC TGATGATGAA
    ACTTTTGAGA AGAACTTTGA GAGAGAAACA CATAAAATAA GTGAGCAAAA TGATGCTGAT
    AATGCTAGTG TATCTTCAAC TTGTGAGCCT TTTGCAACAG ATGTTCTGGG TGACACTGTG
    ATTCACTTAG ATCAGGCATT AGCCAGAATG AGAGACTACA CGCATATGAA GATGGAGGCT
    GAAAGTAGTC CAGACGTCAG GTGCACCTGC AGGATCGTTG AGGATGACGA TGGTGCTGCT
    GCCACGACCA CGATTAGTAA TAATTTAGAA GAAACTCCTG TTATTGAAAA TCATAGTTCC
    CAGCAACCCG TGAGTGAAGT TGCTACCGTC CCGTGTCCTA GAATTGATAC TCAGCAACTG
    GACCGGCAAA TTAAAGCAAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT
    GAAGTATGTT CTTCTCAACT TCTAACCTCA GTAAGACGCA TGGTTTTGGT CCTTACTCAG
    CAAAACAATG AGAGCAAAGA GTTTGTAAAG TTCTTTCATA AGCAGCTTGG AAGTATATTA
    CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAGG ATTGTGGAGA AGATCTTCTT
    GTAGAGATAT CTGAAGTGTT ATTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG
    GATAATAATA GTGTAACTGT TAAACAGAGA TGCAAAAGGA AAATAGAAGC TGCTGGAGTG
    ATACAGTCTT ATGCAAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCACAG TTCACCTGCT
    GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAGACGG AAACAGTTAA GCAGACTCAA
    ACGTCTGAGG TTTATGATGG TGACGGCCCC AAAAATGTGA GATCTGATAT TTCTGATCAA
    GAGGATGATG AAGAAAGTGA AGAATGTCCA GTGTCTATTA GTTTGTCCAA AGCTGAAACT
    CAGGCTTTGA CTAATTACGG AAGTGGAGAA GATGAGAATG AAGATGAAGA GATAGAAGAA
    TTTGAAGAGG GACCTGTAGA TGTCCAGACG TCACTTCAGG CTAACACTGA AACTACAGAA
    GAAAATGAAC ACGATGATCA GGTTCCACAG CATGATTTTG AAAAATCATC AGAAAGCAAA
    AATGTCCCAT CAGAACAAGA ACCCACTACT AGTAAAGGTG ACCAGGATAG CACTCCTGTG
    AAGCCATGTT ACCTCAATAT CGTGGAAAAT GAGCAACCTT TAAATAGCCC TGTCCAGAAG
    GACACTCTCA CTACCATCGA CTCGTCCAGT CAACCTAACC CTTTGCCTTT ACCTTTAACT
    GAAATTGAAA CCTTGGTGCC TAGAGTCAAA GAAGTGAAAT CTGCTCAGGA GACTCCTGAA
    AGTTCTTTGG CTGGAAGTCC TGATACTGAA TCTCCAGTGT TAGTGAATGA CTATGAAGCA
    GAATCTGGTA ACATAAGTCA AAAATCTGAT GAAGAAGACT TTGTGAAAGT TGAAGATTTA
    CCACTTAAAC TGACAATATA TTCAGAGGCA GATCTAAGGA AGAAAATGGC CGAAGAAGAA
    CAGAAAAACC ACTTATCTGG TGAAATATGT GCAATGCAGA CTGAAGAATT AGCTGGGAAT
    TCTCAGACAC TAAAAGAACC TGAAACAGTG GGAGCCCAAA GTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842195.1
    CDS359..6706
    Translation

    Target ORF information:

    RefSeq Version XM_024986427.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986427.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG CAGATTGTCG ATACAATAGA
    GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGATG AGGAGGAGGA GGAGGAGGAA
    GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT
    GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG
    AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA
    GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC
    ACTAGAGGAA ATGCCAATAA AACACAAAAA GATGCTAGAG TAAATGAAAA GGCAAGAGAG
    AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA
    AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC
    CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT
    ACTTCATCCC CAACCGTTAA TGAGAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT
    CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA
    AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG
    AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT
    GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT
    TCTACCCAGT GTGCCCTAGA TGAAGAAGGG GATGAAGATG GTTACCTTTC TGAAGGAATT
    ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT
    CATATGTATC CTCCAAACAG TGCAAATCGT AATTCTTACA ATGTAAAGGA AACTAAAAAT
    AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA
    AGACAACAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT
    TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT
    TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA
    CAAACTCTTC TCACAAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG
    CACCTTATAA TGCACCAGTT GAACCAGTGC TATACCCAGC TAACGTGGCA GCAGAATAAT
    GTACAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCACC AACAAAATCA GCATCCAGAA
    AAACCTGCAA GCAAGGAAGG AGGCAGTGGT GCATCACACC CTCCTTCTCC CAGTTTATTT
    TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG ATTTACTAAT
    TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT
    TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA
    GGGAAAACAG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCA
    GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG
    TTTTATGACC AAGAAGGTGA GGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT
    GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC
    CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA
    ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA
    GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTG CTCAGCCGAA AAGCAGAGTG
    AAAAATATTG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA
    AACAGACATT CAGCCCAGAC CGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAA
    CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGCACAT
    GCTAGGAGAA TACTACAGCA GTCTAACAGA AATGCATGCA ATGAAGCGCC AGAAACCAGT
    AGTGATTTTT CCATGTTCGA AGCTTTGCGG GATACCATTT ATTCTGAAGT AGCTACGTTG
    ATTTCTCAAA ATGAATCTCG TCCACATTTT CTTATTGAAC TCTTCCATGA GCTTCAGCTA
    CTTAATACAG ACTATTTGAG ACAGAGGGCC TTATATGCAT TGCAGGACAT AGTATCCAGG
    CATATTTCTG AGAACCATGA AAAAGAAGGA GAAAATATAA AGTCCGTGAA TTCTGGTACT
    TGGATAGCAT CCAACTCAGA ACTTACTCCC AGTGAAAGCC TTGCTACTAC TGATGATGAA
    ACTTTTGAGA AGAACTTTGA GAGAGAAACA CATAAAATAA GTGAGCAAAA TGATGCTGAT
    AATGCTAGTG TATCTTCAAC TTGTGAGCCT TTTGCAACAG ATGTTCTGGG TGACACTGTG
    ATTCACTTAG ATCAGGCATT AGCCAGAATG AGAGACTACA CGCATATGAA GATGGAGGCT
    GAAAGTAGTC CAGACGTCAG GTGCACCTGC AGGATCGTTG AGGATGACGA TGGTGCTGCT
    GCCACGACCA CGATTAGTAA TAATTTAGAA GAAACTCCTG TTATTGAAAA TCATAGTTCC
    CAGCAACCCG TGAGTGAAGT TGCTACCGTC CCGTGTCCTA GAATTGATAC TCAGCAACTG
    GACCGGCAAA TTAAAGCAAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT
    GAAGTATGTT CTTCTCAACT TCTAACCTCA GTAAGACGCA TGGTTTTGGT CCTTACTCAG
    CAAAACAATG AGAGCAAAGA GTTTGTAAAG TTCTTTCATA AGCAGCTTGG AAGTATATTA
    CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAGG ATTGTGGAGA AGATCTTCTT
    GTAGAGATAT CTGAAGTGTT ATTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG
    GATAATAATA GTGTAACTGT TAAACAGAGA TGCAAAAGGA AAATAGAAGC TGCTGGAGTG
    ATACAGTCTT ATGCAAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCACAG TTCACCTGCT
    GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAGACGG AAACAGTTAA GCAGACTCAA
    ACGTCTGAGG TTTATGATGG TGACGGCCCC AAAAATGTGA GATCTGATAT TTCTGATCAA
    GAGGATGATG AAGAAAGTGA AGAATGTCCA GTGTCTATTA GTTTGTCCAA AGCTGAAACT
    CAGGCTTTGA CTAATTACGG AAGTGGAGAA GATGAGAATG AAGATGAAGA GATAGAAGAA
    TTTGAAGAGG GACCTGTAGA TGTCCAGACG TCACTTCAGG CTAACACTGA AACTACAGAA
    GAAAATGAAC ACGATGATCA GGTTCCACAG CATGATTTTG AAAAATCATC AGAAAGCAAA
    AATGTCCCAT CAGAACAAGA ACCCACTACT AGTAAAGGTG ACCAGGATAG CACTCCTGTG
    AAGCCATGTT ACCTCAATAT CGTGGAAAAT GAGCAACCTT TAAATAGCCC TGTCCAGAAG
    GACACTCTCA CTACCATCGA CTCGTCCAGT CAACCTAACC CTTTGCCTTT ACCTTTAACT
    GAAATTGAAA CCTTGGTGCC TAGAGTCAAA GAAGTGAAAT CTGCTCAGGA GACTCCTGAA
    AGTTCTTTGG CTGGAAGTCC TGATACTGAA TCTCCAGTGT TAGTGAATGA CTATGAAGCA
    GAATCTGGTA ACATAAGTCA AAAATCTGAT GAAGAAGACT TTGTGAAAGT TGAAGATTTA
    CCACTTAAAC TGACAATATA TTCAGAGGCA GATCTAAGGA AGAAAATGGC CGAAGAAGAA
    CAGAAAAACC ACTTATCTGG TGAAATATGT GCAATGCAGA CTGAAGAATT AGCTGGGAAT
    TCTCAGACAC TAAAAGAACC TGAAACAGTG GGAGCCCAAA GTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50980
    Clone ID Related Accession (Same CDS sequence) XM_010826077.2 , XM_010820273.3 , XM_010826078.2 , XM_010820273.2 , XM_010820274.2
    Accession Version XM_010826077.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6555bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646718.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826077.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA
    GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824379.1
    CDS465..7019
    Translation

    Target ORF information:

    RefSeq Version XM_010826077.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826077.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA
    GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50980
    Clone ID Related Accession (Same CDS sequence) XM_010826077.2 , XM_010820273.3 , XM_010826078.2 , XM_010820273.2 , XM_010820274.2
    Accession Version XM_010820273.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6555bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820273.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA
    GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818575.1
    CDS359..6913
    Translation

    Target ORF information:

    RefSeq Version XM_010820273.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820273.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA
    GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50980
    Clone ID Related Accession (Same CDS sequence) XM_010826077.2 , XM_010820273.3 , XM_010826078.2 , XM_010820273.2 , XM_010820274.2
    Accession Version XM_010826078.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6555bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646718.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826078.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA
    GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824380.1
    CDS394..6948
    Translation

    Target ORF information:

    RefSeq Version XM_010826078.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826078.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA
    GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50980
    Clone ID Related Accession (Same CDS sequence) XM_010826077.2 , XM_010820273.3 , XM_010826078.2 , XM_010820273.2 , XM_010820274.2
    Accession Version XM_010820273.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6555bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA
    GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818575.1
    CDS465..7019
    Translation

    Target ORF information:

    RefSeq Version XM_010820273.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820273.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA
    GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50980
    Clone ID Related Accession (Same CDS sequence) XM_010826077.2 , XM_010820273.3 , XM_010826078.2 , XM_010820273.2 , XM_010820274.2
    Accession Version XM_010820274.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6555bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA
    GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818576.1
    CDS394..6948
    Translation

    Target ORF information:

    RefSeq Version XM_010820274.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820274.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA
    GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50981
    Clone ID Related Accession (Same CDS sequence) XM_010826079.2 , XM_010820275.2
    Accession Version XM_010826079.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646718.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826079.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GCACATGCTA GGAGAATACT ACAGCAGTCT AACAGAAATG CATGCAATGA AGCGCCAGAA
    ACCAGTAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT
    ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT
    CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA
    TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT
    GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT
    GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT
    GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGTGAC
    ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTACACGCA TATGAAGATG
    GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCGTTGAGGA TGACGATGGT
    GCTGCTGCCA CGACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT
    AGTTCCCAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG
    CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC
    ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT
    ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT
    ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT
    CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA
    GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT
    GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA
    CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGACTTTCT TTTGATGACA
    GGTGACCAGG ATAGCACTCC TGTGAAGCCA TGTTACCTCA ATATCGTGGA AAATGAGCAA
    CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCGTC CAGTCAACCT
    AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG
    AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA
    GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA
    GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA
    AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG
    CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAAAC AGTGGGAGCC
    CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824381.1
    CDS464..7015
    Translation

    Target ORF information:

    RefSeq Version XM_010826079.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826079.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GCACATGCTA GGAGAATACT ACAGCAGTCT AACAGAAATG CATGCAATGA AGCGCCAGAA
    ACCAGTAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT
    ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT
    CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA
    TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT
    GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT
    GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT
    GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGTGAC
    ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTACACGCA TATGAAGATG
    GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCGTTGAGGA TGACGATGGT
    GCTGCTGCCA CGACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT
    AGTTCCCAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG
    CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC
    ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT
    ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT
    ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT
    CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA
    GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT
    GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA
    CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGACTTTCT TTTGATGACA
    GGTGACCAGG ATAGCACTCC TGTGAAGCCA TGTTACCTCA ATATCGTGGA AAATGAGCAA
    CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCGTC CAGTCAACCT
    AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG
    AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA
    GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA
    GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA
    AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG
    CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAAAC AGTGGGAGCC
    CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50981
    Clone ID Related Accession (Same CDS sequence) XM_010826079.2 , XM_010820275.2
    Accession Version XM_010820275.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820275.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GCACATGCTA GGAGAATACT ACAGCAGTCT AACAGAAATG CATGCAATGA AGCGCCAGAA
    ACCAGTAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT
    ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT
    CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA
    TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT
    GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT
    GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT
    GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGTGAC
    ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTACACGCA TATGAAGATG
    GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCGTTGAGGA TGACGATGGT
    GCTGCTGCCA CGACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT
    AGTTCCCAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG
    CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC
    ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT
    ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT
    ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT
    CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA
    GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT
    GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA
    CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGACTTTCT TTTGATGACA
    GGTGACCAGG ATAGCACTCC TGTGAAGCCA TGTTACCTCA ATATCGTGGA AAATGAGCAA
    CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCGTC CAGTCAACCT
    AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG
    AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA
    GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA
    GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA
    AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG
    CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAAAC AGTGGGAGCC
    CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818577.1
    CDS464..7015
    Translation

    Target ORF information:

    RefSeq Version XM_010820275.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820275.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GCACATGCTA GGAGAATACT ACAGCAGTCT AACAGAAATG CATGCAATGA AGCGCCAGAA
    ACCAGTAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT
    ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT
    CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA
    TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT
    GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT
    GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT
    GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGTGAC
    ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTACACGCA TATGAAGATG
    GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCGTTGAGGA TGACGATGGT
    GCTGCTGCCA CGACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT
    AGTTCCCAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG
    CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC
    ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT
    ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT
    ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT
    CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA
    GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT
    GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA
    CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGACTTTCT TTTGATGACA
    GGTGACCAGG ATAGCACTCC TGTGAAGCCA TGTTACCTCA ATATCGTGGA AAATGAGCAA
    CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCGTC CAGTCAACCT
    AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG
    AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA
    GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA
    GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA
    AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG
    CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAAAC AGTGGGAGCC
    CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50982
    Clone ID Related Accession (Same CDS sequence) XM_010820276.2 , XM_010826080.2 , XM_010820276.3
    Accession Version XM_010820276.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT
    GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC
    GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA
    GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818578.1
    CDS463..6999
    Translation

    Target ORF information:

    RefSeq Version XM_010820276.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820276.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT
    GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC
    GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA
    GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50982
    Clone ID Related Accession (Same CDS sequence) XM_010820276.2 , XM_010826080.2 , XM_010820276.3
    Accession Version XM_010826080.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646718.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT
    GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC
    GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA
    GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824382.1
    CDS463..6999
    Translation

    Target ORF information:

    RefSeq Version XM_010826080.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826080.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT
    GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC
    GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA
    GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50982
    Clone ID Related Accession (Same CDS sequence) XM_010820276.2 , XM_010826080.2 , XM_010820276.3
    Accession Version XM_010820276.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820276.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT
    GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC
    GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA
    GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818578.1
    CDS359..6895
    Translation

    Target ORF information:

    RefSeq Version XM_010820276.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820276.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT
    GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC
    GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA
    GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50983
    Clone ID Related Accession (Same CDS sequence) XM_010826081.2 , XM_010820277.2
    Accession Version XM_010826081.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646718.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGAAACCA GTAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA
    GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT
    GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC
    ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG
    AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT
    ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA
    AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG
    GGTGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA CACGCATATG
    AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCGT TGAGGATGAC
    GATGGTGCTG CTGCCACGAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA
    AATCATAGTT CCCAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT
    ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG
    GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG
    GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT
    GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA
    GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC
    ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA
    GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC
    AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT
    AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT
    ATTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAGTTTGTCC
    AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA
    GAGATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT
    GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA
    TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG
    ATGACAGGTG ACCAGGATAG CACTCCTGTG AAGCCATGTT ACCTCAATAT CGTGGAAAAT
    GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCGTCCAGT
    CAACCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA
    GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA
    TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT
    GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA
    GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT
    GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAAACAGTG
    GGAGCCCAAA GTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824383.1
    CDS465..6962
    Translation

    Target ORF information:

    RefSeq Version XM_010826081.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826081.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGAAACCA GTAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA
    GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT
    GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC
    ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG
    AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT
    ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA
    AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG
    GGTGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA CACGCATATG
    AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCGT TGAGGATGAC
    GATGGTGCTG CTGCCACGAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA
    AATCATAGTT CCCAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT
    ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG
    GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG
    GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT
    GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA
    GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC
    ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA
    GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC
    AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT
    AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT
    ATTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAGTTTGTCC
    AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA
    GAGATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT
    GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA
    TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG
    ATGACAGGTG ACCAGGATAG CACTCCTGTG AAGCCATGTT ACCTCAATAT CGTGGAAAAT
    GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCGTCCAGT
    CAACCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA
    GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA
    TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT
    GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA
    GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT
    GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAAACAGTG
    GGAGCCCAAA GTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50983
    Clone ID Related Accession (Same CDS sequence) XM_010826081.2 , XM_010820277.2
    Accession Version XM_010820277.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820277.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGAAACCA GTAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA
    GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT
    GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC
    ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG
    AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT
    ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA
    AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG
    GGTGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA CACGCATATG
    AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCGT TGAGGATGAC
    GATGGTGCTG CTGCCACGAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA
    AATCATAGTT CCCAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT
    ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG
    GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG
    GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT
    GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA
    GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC
    ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA
    GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC
    AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT
    AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT
    ATTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAGTTTGTCC
    AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA
    GAGATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT
    GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA
    TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG
    ATGACAGGTG ACCAGGATAG CACTCCTGTG AAGCCATGTT ACCTCAATAT CGTGGAAAAT
    GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCGTCCAGT
    CAACCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA
    GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA
    TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT
    GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA
    GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT
    GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAAACAGTG
    GGAGCCCAAA GTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818579.1
    CDS465..6962
    Translation

    Target ORF information:

    RefSeq Version XM_010820277.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820277.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGAAACCA GTAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA
    GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT
    GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC
    ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG
    AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT
    ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA
    AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG
    GGTGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA CACGCATATG
    AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCGT TGAGGATGAC
    GATGGTGCTG CTGCCACGAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA
    AATCATAGTT CCCAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT
    ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG
    GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG
    GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT
    GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA
    GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC
    ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA
    GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC
    AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT
    AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT
    ATTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAGTTTGTCC
    AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA
    GAGATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT
    GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA
    TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGA CTTTCTTTTG
    ATGACAGGTG ACCAGGATAG CACTCCTGTG AAGCCATGTT ACCTCAATAT CGTGGAAAAT
    GAGCAACCTT TAAATAGCCC TGTCCAGAAG GACACTCTCA CTACCATCGA CTCGTCCAGT
    CAACCTAACC CTTTGCCTTT ACCTTTAACT GAAATTGAAA CCTTGGTGCC TAGAGTCAAA
    GAAGTGAAAT CTGCTCAGGA GACTCCTGAA AGTTCTTTGG CTGGAAGTCC TGATACTGAA
    TCTCCAGTGT TAGTGAATGA CTATGAAGCA GAATCTGGTA ACATAAGTCA AAAATCTGAT
    GAAGAAGACT TTGTGAAAGT TGAAGATTTA CCACTTAAAC TGACAATATA TTCAGAGGCA
    GATCTAAGGA AGAAAATGGC CGAAGAAGAA CAGAAAAACC ACTTATCTGG TGAAATATGT
    GCAATGCAGA CTGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAAACAGTG
    GGAGCCCAAA GTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50984
    Clone ID Related Accession (Same CDS sequence) XM_010820278.2 , XM_010826082.2
    Accession Version XM_010820278.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820278.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA
    GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818580.1
    CDS465..6959
    Translation

    Target ORF information:

    RefSeq Version XM_010820278.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820278.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA
    GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50984
    Clone ID Related Accession (Same CDS sequence) XM_010820278.2 , XM_010826082.2
    Accession Version XM_010826082.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646718.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA
    GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824384.1
    CDS465..6959
    Translation

    Target ORF information:

    RefSeq Version XM_010826082.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826082.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA
    GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATATTC AGAGGCAGAT
    CTAAGGAAGA AAATGGCCGA AGAAGAACAG AAAAACCACT TATCTGGTGA AATATGTGCA
    ATGCAGACTG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA
    GCCCAAAGTA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50985
    Clone ID Related Accession (Same CDS sequence) XM_010820279.2 , XM_010826083.2
    Accession Version XM_010820279.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820279.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGACTTTCT TTTGATGACA
    GGTGACCAGG ATAGCACTCC TGTGAAGCCA TGTTACCTCA ATATCGTGGA AAATGAGCAA
    CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCGTC CAGTCAACCT
    AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG
    AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA
    GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA
    GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA
    AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG
    CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAAAC AGTGGGAGCC
    CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818581.1
    CDS466..6957
    Translation

    Target ORF information:

    RefSeq Version XM_010820279.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820279.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGACTTTCT TTTGATGACA
    GGTGACCAGG ATAGCACTCC TGTGAAGCCA TGTTACCTCA ATATCGTGGA AAATGAGCAA
    CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCGTC CAGTCAACCT
    AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG
    AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA
    GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA
    GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA
    AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG
    CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAAAC AGTGGGAGCC
    CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50985
    Clone ID Related Accession (Same CDS sequence) XM_010820279.2 , XM_010826083.2
    Accession Version XM_010826083.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646718.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826083.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGACTTTCT TTTGATGACA
    GGTGACCAGG ATAGCACTCC TGTGAAGCCA TGTTACCTCA ATATCGTGGA AAATGAGCAA
    CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCGTC CAGTCAACCT
    AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG
    AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA
    GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA
    GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA
    AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG
    CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAAAC AGTGGGAGCC
    CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824385.1
    CDS466..6957
    Translation

    Target ORF information:

    RefSeq Version XM_010826083.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826083.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGACTTTCT TTTGATGACA
    GGTGACCAGG ATAGCACTCC TGTGAAGCCA TGTTACCTCA ATATCGTGGA AAATGAGCAA
    CCTTTAAATA GCCCTGTCCA GAAGGACACT CTCACTACCA TCGACTCGTC CAGTCAACCT
    AACCCTTTGC CTTTACCTTT AACTGAAATT GAAACCTTGG TGCCTAGAGT CAAAGAAGTG
    AAATCTGCTC AGGAGACTCC TGAAAGTTCT TTGGCTGGAA GTCCTGATAC TGAATCTCCA
    GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA
    GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATATTCAGA GGCAGATCTA
    AGGAAGAAAA TGGCCGAAGA AGAACAGAAA AACCACTTAT CTGGTGAAAT ATGTGCAATG
    CAGACTGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAAAC AGTGGGAGCC
    CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50986
    Clone ID Related Accession (Same CDS sequence) XM_010826084.2 , XM_010820280.3 , XM_010820280.2
    Accession Version XM_010826084.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6480bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646718.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826084.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGAAACCA GTAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA
    GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT
    GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC
    ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG
    AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT
    ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA
    AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG
    GGTGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA CACGCATATG
    AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCGT TGAGGATGAC
    GATGGTGCTG CTGCCACGAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA
    AATCATAGTT CCCAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT
    ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG
    GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG
    GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT
    GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA
    GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC
    ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA
    GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC
    AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT
    AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT
    ATTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAGTTTGTCC
    AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA
    GAGATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT
    GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA
    TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGG TGACCAGGAT
    AGCACTCCTG TGAAGCCATG TTACCTCAAT ATCGTGGAAA ATGAGCAACC TTTAAATAGC
    CCTGTCCAGA AGGACACTCT CACTACCATC GACTCGTCCA GTCAACCTAA CCCTTTGCCT
    TTACCTTTAA CTGAAATTGA AACCTTGGTG CCTAGAGTCA AAGAAGTGAA ATCTGCTCAG
    GAGACTCCTG AAAGTTCTTT GGCTGGAAGT CCTGATACTG AATCTCCAGT GTTAGTGAAT
    GACTATGAAG CAGAATCTGG TAACATAAGT CAAAAATCTG ATGAAGAAGA CTTTGTGAAA
    GTTGAAGATT TACCACTTAA ACTGACAATA TATTCAGAGG CAGATCTAAG GAAGAAAATG
    GCCGAAGAAG AACAGAAAAA CCACTTATCT GGTGAAATAT GTGCAATGCA GACTGAAGAA
    TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824386.1
    CDS463..6942
    Translation

    Target ORF information:

    RefSeq Version XM_010826084.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826084.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGAAACCA GTAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA
    GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT
    GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC
    ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG
    AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT
    ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA
    AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG
    GGTGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA CACGCATATG
    AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCGT TGAGGATGAC
    GATGGTGCTG CTGCCACGAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA
    AATCATAGTT CCCAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT
    ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG
    GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG
    GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT
    GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA
    GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC
    ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA
    GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC
    AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT
    AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT
    ATTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAGTTTGTCC
    AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA
    GAGATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT
    GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA
    TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGG TGACCAGGAT
    AGCACTCCTG TGAAGCCATG TTACCTCAAT ATCGTGGAAA ATGAGCAACC TTTAAATAGC
    CCTGTCCAGA AGGACACTCT CACTACCATC GACTCGTCCA GTCAACCTAA CCCTTTGCCT
    TTACCTTTAA CTGAAATTGA AACCTTGGTG CCTAGAGTCA AAGAAGTGAA ATCTGCTCAG
    GAGACTCCTG AAAGTTCTTT GGCTGGAAGT CCTGATACTG AATCTCCAGT GTTAGTGAAT
    GACTATGAAG CAGAATCTGG TAACATAAGT CAAAAATCTG ATGAAGAAGA CTTTGTGAAA
    GTTGAAGATT TACCACTTAA ACTGACAATA TATTCAGAGG CAGATCTAAG GAAGAAAATG
    GCCGAAGAAG AACAGAAAAA CCACTTATCT GGTGAAATAT GTGCAATGCA GACTGAAGAA
    TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50986
    Clone ID Related Accession (Same CDS sequence) XM_010826084.2 , XM_010820280.3 , XM_010820280.2
    Accession Version XM_010820280.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6480bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820280.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGAAACCA GTAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA
    GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT
    GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC
    ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG
    AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT
    ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA
    AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG
    GGTGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA CACGCATATG
    AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCGT TGAGGATGAC
    GATGGTGCTG CTGCCACGAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA
    AATCATAGTT CCCAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT
    ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG
    GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG
    GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT
    GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA
    GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC
    ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA
    GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC
    AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT
    AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT
    ATTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAGTTTGTCC
    AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA
    GAGATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT
    GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA
    TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGG TGACCAGGAT
    AGCACTCCTG TGAAGCCATG TTACCTCAAT ATCGTGGAAA ATGAGCAACC TTTAAATAGC
    CCTGTCCAGA AGGACACTCT CACTACCATC GACTCGTCCA GTCAACCTAA CCCTTTGCCT
    TTACCTTTAA CTGAAATTGA AACCTTGGTG CCTAGAGTCA AAGAAGTGAA ATCTGCTCAG
    GAGACTCCTG AAAGTTCTTT GGCTGGAAGT CCTGATACTG AATCTCCAGT GTTAGTGAAT
    GACTATGAAG CAGAATCTGG TAACATAAGT CAAAAATCTG ATGAAGAAGA CTTTGTGAAA
    GTTGAAGATT TACCACTTAA ACTGACAATA TATTCAGAGG CAGATCTAAG GAAGAAAATG
    GCCGAAGAAG AACAGAAAAA CCACTTATCT GGTGAAATAT GTGCAATGCA GACTGAAGAA
    TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818582.1
    CDS359..6838
    Translation

    Target ORF information:

    RefSeq Version XM_010820280.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820280.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGAAACCA GTAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA
    GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT
    GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC
    ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG
    AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT
    ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA
    AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG
    GGTGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA CACGCATATG
    AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCGT TGAGGATGAC
    GATGGTGCTG CTGCCACGAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA
    AATCATAGTT CCCAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT
    ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG
    GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG
    GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT
    GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA
    GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC
    ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA
    GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC
    AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT
    AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT
    ATTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAGTTTGTCC
    AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA
    GAGATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT
    GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA
    TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGG TGACCAGGAT
    AGCACTCCTG TGAAGCCATG TTACCTCAAT ATCGTGGAAA ATGAGCAACC TTTAAATAGC
    CCTGTCCAGA AGGACACTCT CACTACCATC GACTCGTCCA GTCAACCTAA CCCTTTGCCT
    TTACCTTTAA CTGAAATTGA AACCTTGGTG CCTAGAGTCA AAGAAGTGAA ATCTGCTCAG
    GAGACTCCTG AAAGTTCTTT GGCTGGAAGT CCTGATACTG AATCTCCAGT GTTAGTGAAT
    GACTATGAAG CAGAATCTGG TAACATAAGT CAAAAATCTG ATGAAGAAGA CTTTGTGAAA
    GTTGAAGATT TACCACTTAA ACTGACAATA TATTCAGAGG CAGATCTAAG GAAGAAAATG
    GCCGAAGAAG AACAGAAAAA CCACTTATCT GGTGAAATAT GTGCAATGCA GACTGAAGAA
    TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50986
    Clone ID Related Accession (Same CDS sequence) XM_010826084.2 , XM_010820280.3 , XM_010820280.2
    Accession Version XM_010820280.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6480bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820280.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGAAACCA GTAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA
    GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT
    GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC
    ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG
    AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT
    ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA
    AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG
    GGTGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA CACGCATATG
    AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCGT TGAGGATGAC
    GATGGTGCTG CTGCCACGAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA
    AATCATAGTT CCCAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT
    ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG
    GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG
    GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT
    GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA
    GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC
    ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA
    GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC
    AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT
    AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT
    ATTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAGTTTGTCC
    AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA
    GAGATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT
    GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA
    TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGG TGACCAGGAT
    AGCACTCCTG TGAAGCCATG TTACCTCAAT ATCGTGGAAA ATGAGCAACC TTTAAATAGC
    CCTGTCCAGA AGGACACTCT CACTACCATC GACTCGTCCA GTCAACCTAA CCCTTTGCCT
    TTACCTTTAA CTGAAATTGA AACCTTGGTG CCTAGAGTCA AAGAAGTGAA ATCTGCTCAG
    GAGACTCCTG AAAGTTCTTT GGCTGGAAGT CCTGATACTG AATCTCCAGT GTTAGTGAAT
    GACTATGAAG CAGAATCTGG TAACATAAGT CAAAAATCTG ATGAAGAAGA CTTTGTGAAA
    GTTGAAGATT TACCACTTAA ACTGACAATA TATTCAGAGG CAGATCTAAG GAAGAAAATG
    GCCGAAGAAG AACAGAAAAA CCACTTATCT GGTGAAATAT GTGCAATGCA GACTGAAGAA
    TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818582.1
    CDS463..6942
    Translation

    Target ORF information:

    RefSeq Version XM_010820280.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820280.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGAAACCA GTAGTGATTT TTCCATGTTC GAAGCTTTGC GGGATACCAT TTATTCTGAA
    GTAGCTACGT TGATTTCTCA AAATGAATCT CGTCCACATT TTCTTATTGA ACTCTTCCAT
    GAGCTTCAGC TACTTAATAC AGACTATTTG AGACAGAGGG CCTTATATGC ATTGCAGGAC
    ATAGTATCCA GGCATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATAT AAAGTCCGTG
    AATTCTGGTA CTTGGATAGC ATCCAACTCA GAACTTACTC CCAGTGAAAG CCTTGCTACT
    ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA
    AATGATGCTG ATAATGCTAG TGTATCTTCA ACTTGTGAGC CTTTTGCAAC AGATGTTCTG
    GGTGACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGAGACTA CACGCATATG
    AAGATGGAGG CTGAAAGTAG TCCAGACGTC AGGTGCACCT GCAGGATCGT TGAGGATGAC
    GATGGTGCTG CTGCCACGAC CACGATTAGT AATAATTTAG AAGAAACTCC TGTTATTGAA
    AATCATAGTT CCCAGCAACC CGTGAGTGAA GTTGCTACCG TCCCGTGTCC TAGAATTGAT
    ACTCAGCAAC TGGACCGGCA AATTAAAGCA ATTATGAAAG AAGTCATTCC TTTTTTGAAG
    GAGCACATGG ATGAAGTATG TTCTTCTCAA CTTCTAACCT CAGTAAGACG CATGGTTTTG
    GTCCTTACTC AGCAAAACAA TGAGAGCAAA GAGTTTGTAA AGTTCTTTCA TAAGCAGCTT
    GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA GGATTGTGGA
    GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTATTTAATG AATTGGCTTT CTTTAAGCTC
    ATGCAAGATT TGGATAATAA TAGTGTAACT GTTAAACAGA GATGCAAAAG GAAAATAGAA
    GCTGCTGGAG TGATACAGTC TTATGCAAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAC
    AGTTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAGAC GGAAACAGTT
    AAGCAGACTC AAACGTCTGA GGTTTATGAT GGTGACGGCC CCAAAAATGT GAGATCTGAT
    ATTTCTGATC AAGAGGATGA TGAAGAAAGT GAAGAATGTC CAGTGTCTAT TAGTTTGTCC
    AAAGCTGAAA CTCAGGCTTT GACTAATTAC GGAAGTGGAG AAGATGAGAA TGAAGATGAA
    GAGATAGAAG AATTTGAAGA GGGACCTGTA GATGTCCAGA CGTCACTTCA GGCTAACACT
    GAAACTACAG AAGAAAATGA ACACGATGAT CAGGTTCCAC AGCATGATTT TGAAAAATCA
    TCAGAAAGCA AAAATGTCCC ATCAGAACAA GAACCCACTA CTAGTAAAGG TGACCAGGAT
    AGCACTCCTG TGAAGCCATG TTACCTCAAT ATCGTGGAAA ATGAGCAACC TTTAAATAGC
    CCTGTCCAGA AGGACACTCT CACTACCATC GACTCGTCCA GTCAACCTAA CCCTTTGCCT
    TTACCTTTAA CTGAAATTGA AACCTTGGTG CCTAGAGTCA AAGAAGTGAA ATCTGCTCAG
    GAGACTCCTG AAAGTTCTTT GGCTGGAAGT CCTGATACTG AATCTCCAGT GTTAGTGAAT
    GACTATGAAG CAGAATCTGG TAACATAAGT CAAAAATCTG ATGAAGAAGA CTTTGTGAAA
    GTTGAAGATT TACCACTTAA ACTGACAATA TATTCAGAGG CAGATCTAAG GAAGAAAATG
    GCCGAAGAAG AACAGAAAAA CCACTTATCT GGTGAAATAT GTGCAATGCA GACTGAAGAA
    TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50987
    Clone ID Related Accession (Same CDS sequence) XM_010820281.2 , XM_010826085.2 , XM_010820281.3
    Accession Version XM_010820281.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820281.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT
    GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC
    GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA
    GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818583.1
    CDS463..6939
    Translation

    Target ORF information:

    RefSeq Version XM_010820281.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820281.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT
    GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC
    GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA
    GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50987
    Clone ID Related Accession (Same CDS sequence) XM_010820281.2 , XM_010826085.2 , XM_010820281.3
    Accession Version XM_010826085.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646718.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT
    GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC
    GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA
    GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824387.1
    CDS463..6939
    Translation

    Target ORF information:

    RefSeq Version XM_010826085.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826085.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT
    GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC
    GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA
    GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50987
    Clone ID Related Accession (Same CDS sequence) XM_010820281.2 , XM_010826085.2 , XM_010820281.3
    Accession Version XM_010820281.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820281.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT
    GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC
    GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA
    GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818583.1
    CDS359..6835
    Translation

    Target ORF information:

    RefSeq Version XM_010820281.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820281.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGA TTGTCGATAC
    AATAGAGAAG GAGAACAGGA GATGCCTGTT GCACAAGGTC ATGATGAGGA GGAGGAGGAG
    GAGGAAGCAG AAGATGAGGC CCTCAGTGGA GCTTCACTGT CTAGTCACAG CAGCAGCCTG
    ATCATTGAGA CTCCTGAAGA TGCTGAATTT GAACATAAGA TCCAGAAGCT TATGGCTGCA
    AAACTGAAAC TTAGACACTT ACAGAACCTT GTAGCCATGG TGCAGGACGA TGATGCAGCC
    AATCAAGGAG TTACTTCTGT GGAGGATTTC TTCCCAGCAG AAGACACCAA ACAAAATGCA
    AATAACACTA GAGGAAATGC CAATAAAACA CAAAAAGATG CTAGAGTAAA TGAAAAGGCA
    AGAGAGAAAT TTTATGAGGC TAAACTACAG CAGCAACAGA GAGAACTCAA ACAATTGCAG
    GAAGAAAGAA AGAAACTGAT TGAGATTCAA GAGAAAATTC AAGAATTGCA AAAGGCATGT
    CCTAACCTAC AGCTGTCAGC TGCTAGTGTG GGTAATTGTC CCACAAAAAC ACATATGCCA
    GCTGTTACTT CATCCCCAAC CGTTAATGAG AATGAAGCCA GTACAAACAA ATCTGGTTTT
    GAGCCTCATG ATTCTTCAGT GGTAGATAAT GAGGTATGGT CAGATATGCG AAGACATGAA
    ATGTTAAGGG AGGAGCTACG ACAGAGAAGA AAGCAGCTGG AAGCTCTGAT GGCTGAACAT
    CAGAGGAGGC AAGGTCTGGC TGAAACTGCA TCTCCAGTGG CTGTTTCACT GAGAAGTGAT
    GGATCTGAGA ACCTGTGTAC ACCTCAGCAA AGCAGAACAG AAAAAACAAT GGCAACTTGG
    GGAGGTTCTA CCCAGTGTGC CCTAGATGAA GAAGGGGATG AAGATGGTTA CCTTTCTGAA
    GGAATTATTC GGACAGACGA AGAGGAGGAA GAGGAGGAGC AAGATGCCAG TTCCAATGAT
    AACTTTCATA TGTATCCTCC AAACAGTGCA AATCGTAATT CTTACAATGT AAAGGAAACT
    AAAAATAGGT GGAAGAACAA TCGCCCTTTT TCAGCAGATG GAAATTATCG TCCTTTAGCC
    AAGACAAGAC AACAGAATAT CAGCATGCAG CGTCAGGAAA ACCTTCGCTG GGTGTCCGAA
    CTCTCTTATG TAGAAGAGAA AGAACAATGG CAAGAACAAA TTAATCAGCT AAAGAAACAA
    CTTGATTTCA GTGTCAATAT TTGTCAGACT TTAATGCAAG ACCAGCAGAC TCTGTCTTGT
    CTGCTACAAA CTCTTCTCAC AAGTCCTTAT AGTGTTATGC CCAGCAGTGT TGCATCCCCG
    CAAGTGCACC TTATAATGCA CCAGTTGAAC CAGTGCTATA CCCAGCTAAC GTGGCAGCAG
    AATAATGTAC AGAGGTTGAA ACAAATGCTC AGTGAACTCA TGCACCAACA AAATCAGCAT
    CCAGAAAAAC CTGCAAGCAA GGAAGGAGGC AGTGGTGCAT CACACCCTCC TTCTCCCAGT
    TTATTTTGTC CTTTCAACTT TCCAACTCAG CCTGTAAATC TGTTTAACCT ACCGGGATTT
    ACTAATTTTT CGTCATTTGC ACCAAGTATG AATTTCAGCC CTTTATTTCC TTCTAATTTT
    GGAGATTTTT CTCAAAATAT TTCTACACCC ACTGAACAGC AGCAACCGTT AGCCCAGAAT
    CCTTCAGGGA AAACAGAATA CATGGCTTTT CCAAAACCTT TTGAAAGTAG TTCTTCTATT
    GGAGCAGAAA AACAAAGGAA TCAAAAACAG CCTGAAGAGG AGGTGGAGAA CAGTAGGACA
    CCATGGTTTT ATGACCAAGA AGGTGAGGTA GAAAAGCCAT TTCTCAAGAC TGGATTTGCA
    GTGTCTGTAG AGAAAACAAC AAATAGTAAT CGCAAAAATC AACTAGATAC CAGTAGGAGA
    AGACGCCAGT TTGATGAAGT GTCCTTAGAA AGCTTTAACA GTATGCTTGA CCCAGTAGAT
    CCAACAACAG TAACTAAAAC ATTCAAGTCA AGAAAAGCAT CTGCACAGGC CAGCCTGGCA
    TCTAAAGATA AAACTCCCAA ATCAAAGAGT AAGAAGAGGC ATTCTGCTCA GCCGAAAAGC
    AGAGTGAAAA ATATTGGGTA TGAAAGTGCC AGTGTGTCTA GCACATGTGA ACCTTGCAAA
    AGTAGAAACA GACATTCAGC CCAGACCGAA GAGCCTGTTC AAGCAAAAGT ACTCAGCAGA
    AAGAAACATG AGCAGCTGGA AAAAGTCATA AGATGTAGTA GGTCTACAGA AATATCTTCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGGTGA CCAGGATAGC
    ACTCCTGTGA AGCCATGTTA CCTCAATATC GTGGAAAATG AGCAACCTTT AAATAGCCCT
    GTCCAGAAGG ACACTCTCAC TACCATCGAC TCGTCCAGTC AACCTAACCC TTTGCCTTTA
    CCTTTAACTG AAATTGAAAC CTTGGTGCCT AGAGTCAAAG AAGTGAAATC TGCTCAGGAG
    ACTCCTGAAA GTTCTTTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC
    TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT
    GAAGATTTAC CACTTAAACT GACAATATAT TCAGAGGCAG ATCTAAGGAA GAAAATGGCC
    GAAGAAGAAC AGAAAAACCA CTTATCTGGT GAAATATGTG CAATGCAGAC TGAAGAATTA
    GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50988
    Clone ID Related Accession (Same CDS sequence) XM_010820282.2 , XM_010826086.2 , XM_010820282.3
    Accession Version XM_010820282.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6462bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820282.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGCAGATCTA AGGAAGAAAA TGGCCGAAGA AGAACAGAAA
    AACCACTTAT CTGGTGAAAT ATGTGCAATG CAGACTGAAG AATTAGCTGG GAATTCTCAG
    ACACTAAAAG AACCTGAAAC AGTGGGAGCC CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818584.1
    CDS467..6928
    Translation

    Target ORF information:

    RefSeq Version XM_010820282.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820282.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGCAGATCTA AGGAAGAAAA TGGCCGAAGA AGAACAGAAA
    AACCACTTAT CTGGTGAAAT ATGTGCAATG CAGACTGAAG AATTAGCTGG GAATTCTCAG
    ACACTAAAAG AACCTGAAAC AGTGGGAGCC CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50988
    Clone ID Related Accession (Same CDS sequence) XM_010820282.2 , XM_010826086.2 , XM_010820282.3
    Accession Version XM_010826086.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6462bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646718.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGCAGATCTA AGGAAGAAAA TGGCCGAAGA AGAACAGAAA
    AACCACTTAT CTGGTGAAAT ATGTGCAATG CAGACTGAAG AATTAGCTGG GAATTCTCAG
    ACACTAAAAG AACCTGAAAC AGTGGGAGCC CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824388.1
    CDS467..6928
    Translation

    Target ORF information:

    RefSeq Version XM_010826086.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826086.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGCAGATCTA AGGAAGAAAA TGGCCGAAGA AGAACAGAAA
    AACCACTTAT CTGGTGAAAT ATGTGCAATG CAGACTGAAG AATTAGCTGG GAATTCTCAG
    ACACTAAAAG AACCTGAAAC AGTGGGAGCC CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50988
    Clone ID Related Accession (Same CDS sequence) XM_010820282.2 , XM_010826086.2 , XM_010820282.3
    Accession Version XM_010820282.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6462bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820282.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGCAGATCTA AGGAAGAAAA TGGCCGAAGA AGAACAGAAA
    AACCACTTAT CTGGTGAAAT ATGTGCAATG CAGACTGAAG AATTAGCTGG GAATTCTCAG
    ACACTAAAAG AACCTGAAAC AGTGGGAGCC CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818584.1
    CDS359..6820
    Translation

    Target ORF information:

    RefSeq Version XM_010820282.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820282.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GAGGCATCTT TTAGTCTGAG AATTCGGCCG TGCGTTGAGG ACAAACTAGG GAATTCAGCT
    TCACAAGCAC AGGTTTCAGA CACTGACGTT ACTACAAGTC CAAAAGGGAA AGGTGACAGA
    CCTCCTCAGC ATGACAGGGA GCTGAGGCCT AATAGGAAAT GTAGCCGGAA ACGTGGATTT
    CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATAGAAG AGATAGAAAA TTTGAAGAAA
    CAACATGATT TATTAAAAAG GATGTTACAG CAGCAGGAGC AGCTGAGAGC TCTACAGGGA
    CGACAAGCTG CTCTGCTTGC CCTGCAGCAT AAAGCCGAGC AGGCTATAGC TGTGATGGAT
    GATTCTGAAA AGGTTGCGGA GACAACTCCT GGCCATCATA CTGTACCAGG CAGACAGCCG
    GCTCGTTCAC CTTTGCATCA GAGAGTACCC ATAAGAGAAA CTACAGGTAG CCTATCTGGA
    GTCAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCA TTTTCATAAT
    CAGCTTCGTG ATTCTCAGCC TCCAGCTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT
    TCATTAACTA GGGAGATTTC TCAGAGCAGA AATCCATCAG TTTCAGAGCA TTTACCTGAT
    GAGAAAGTCC AACTTTTTAG CAAGATGAGA GTGTTACAAG AAAAGAAACA AAAAATGGAC
    AAACTTCTTG GAGAACTTCA TACACTTCGA GATCAGCATT TGAACAATTC ATCATTTGTG
    CCTTCTTCAG CCTCTCCACA AAGGAGTGTG GATCAGAGAA GTACAACCAC AGCTCCTTCT
    GCTCCTGTAG TCTTAACACC AGTGGTCAAT GGAGAATCCA ATAACCTCAC GCCATCTGTT
    CCTTATCCTG CTGCTTCTCT AGTTTCTCAG AATCAGAATG AAAATGAAGG CCACCTAAAT
    CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GGTTGAATGA ACTAAGAGAA
    TTAGTTCATT ATTATGAGCA AACATCAGAT ATGATGACAG ATGCTGTGAA TGAAAACACA
    AAAGAAGAAG AAACTGAAGA GTCGGAATAT GATTCTGAGC ATGAAAACTC TGAACCTGTT
    ACTCATATTC GAAATCCACA AGTGGCTGCC ACCTGGAACG AAGTAAATAG TAATTCTAAT
    GCACAGTGTG TTTCTAACAA TAGAGATGGG CGAGCAGTTA ATTCTAACTG TGAAATTAAC
    AACAGGTCTG CTGCCAACAT AAGGGCTCTA AACATGCCTC CTCCTTCAGC AGATTGTCGA
    TACAATAGAG AAGGAGAACA GGAGATGCCT GTTGCACAAG GTCATGATGA GGAGGAGGAG
    GAGGAGGAAG CAGAAGATGA GGCCCTCAGT GGAGCTTCAC TGTCTAGTCA CAGCAGCAGC
    CTGATCATTG AGACTCCTGA AGATGCTGAA TTTGAACATA AGATCCAGAA GCTTATGGCT
    GCAAAACTGA AACTTAGACA CTTACAGAAC CTTGTAGCCA TGGTGCAGGA CGATGATGCA
    GCCAATCAAG GAGTTACTTC TGTGGAGGAT TTCTTCCCAG CAGAAGACAC CAAACAAAAT
    GCAAATAACA CTAGAGGAAA TGCCAATAAA ACACAAAAAG ATGCTAGAGT AAATGAAAAG
    GCAAGAGAGA AATTTTATGA GGCTAAACTA CAGCAGCAAC AGAGAGAACT CAAACAATTG
    CAGGAAGAAA GAAAGAAACT GATTGAGATT CAAGAGAAAA TTCAAGAATT GCAAAAGGCA
    TGTCCTAACC TACAGCTGTC AGCTGCTAGT GTGGGTAATT GTCCCACAAA AACACATATG
    CCAGCTGTTA CTTCATCCCC AACCGTTAAT GAGAATGAAG CCAGTACAAA CAAATCTGGT
    TTTGAGCCTC ATGATTCTTC AGTGGTAGAT AATGAGGTAT GGTCAGATAT GCGAAGACAT
    GAAATGTTAA GGGAGGAGCT ACGACAGAGA AGAAAGCAGC TGGAAGCTCT GATGGCTGAA
    CATCAGAGGA GGCAAGGTCT GGCTGAAACT GCATCTCCAG TGGCTGTTTC ACTGAGAAGT
    GATGGATCTG AGAACCTGTG TACACCTCAG CAAAGCAGAA CAGAAAAAAC AATGGCAACT
    TGGGGAGGTT CTACCCAGTG TGCCCTAGAT GAAGAAGGGG ATGAAGATGG TTACCTTTCT
    GAAGGAATTA TTCGGACAGA CGAAGAGGAG GAAGAGGAGG AGCAAGATGC CAGTTCCAAT
    GATAACTTTC ATATGTATCC TCCAAACAGT GCAAATCGTA ATTCTTACAA TGTAAAGGAA
    ACTAAAAATA GGTGGAAGAA CAATCGCCCT TTTTCAGCAG ATGGAAATTA TCGTCCTTTA
    GCCAAGACAA GACAACAGAA TATCAGCATG CAGCGTCAGG AAAACCTTCG CTGGGTGTCC
    GAACTCTCTT ATGTAGAAGA GAAAGAACAA TGGCAAGAAC AAATTAATCA GCTAAAGAAA
    CAACTTGATT TCAGTGTCAA TATTTGTCAG ACTTTAATGC AAGACCAGCA GACTCTGTCT
    TGTCTGCTAC AAACTCTTCT CACAAGTCCT TATAGTGTTA TGCCCAGCAG TGTTGCATCC
    CCGCAAGTGC ACCTTATAAT GCACCAGTTG AACCAGTGCT ATACCCAGCT AACGTGGCAG
    CAGAATAATG TACAGAGGTT GAAACAAATG CTCAGTGAAC TCATGCACCA ACAAAATCAG
    CATCCAGAAA AACCTGCAAG CAAGGAAGGA GGCAGTGGTG CATCACACCC TCCTTCTCCC
    AGTTTATTTT GTCCTTTCAA CTTTCCAACT CAGCCTGTAA ATCTGTTTAA CCTACCGGGA
    TTTACTAATT TTTCGTCATT TGCACCAAGT ATGAATTTCA GCCCTTTATT TCCTTCTAAT
    TTTGGAGATT TTTCTCAAAA TATTTCTACA CCCACTGAAC AGCAGCAACC GTTAGCCCAG
    AATCCTTCAG GGAAAACAGA ATACATGGCT TTTCCAAAAC CTTTTGAAAG TAGTTCTTCT
    ATTGGAGCAG AAAAACAAAG GAATCAAAAA CAGCCTGAAG AGGAGGTGGA GAACAGTAGG
    ACACCATGGT TTTATGACCA AGAAGGTGAG GTAGAAAAGC CATTTCTCAA GACTGGATTT
    GCAGTGTCTG TAGAGAAAAC AACAAATAGT AATCGCAAAA ATCAACTAGA TACCAGTAGG
    AGAAGACGCC AGTTTGATGA AGTGTCCTTA GAAAGCTTTA ACAGTATGCT TGACCCAGTA
    GATCCAACAA CAGTAACTAA AACATTCAAG TCAAGAAAAG CATCTGCACA GGCCAGCCTG
    GCATCTAAAG ATAAAACTCC CAAATCAAAG AGTAAGAAGA GGCATTCTGC TCAGCCGAAA
    AGCAGAGTGA AAAATATTGG GTATGAAAGT GCCAGTGTGT CTAGCACATG TGAACCTTGC
    AAAAGTAGAA ACAGACATTC AGCCCAGACC GAAGAGCCTG TTCAAGCAAA AGTACTCAGC
    AGAAAGAAAC ATGAGCAGCT GGAAAAAGTC ATAAGATGTA GTAGGTCTAC AGAAATATCT
    TCAGCACATG CTAGGAGAAT ACTACAGCAG TCTAACAGAA ATGCATGCAA TGAAGCGCCA
    GAAACCAGTA GTGATTTTTC CATGTTCGAA GCTTTGCGGG ATACCATTTA TTCTGAAGTA
    GCTACGTTGA TTTCTCAAAA TGAATCTCGT CCACATTTTC TTATTGAACT CTTCCATGAG
    CTTCAGCTAC TTAATACAGA CTATTTGAGA CAGAGGGCCT TATATGCATT GCAGGACATA
    GTATCCAGGC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATATAAA GTCCGTGAAT
    TCTGGTACTT GGATAGCATC CAACTCAGAA CTTACTCCCA GTGAAAGCCT TGCTACTACT
    GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT
    GATGCTGATA ATGCTAGTGT ATCTTCAACT TGTGAGCCTT TTGCAACAGA TGTTCTGGGT
    GACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GAGACTACAC GCATATGAAG
    ATGGAGGCTG AAAGTAGTCC AGACGTCAGG TGCACCTGCA GGATCGTTGA GGATGACGAT
    GGTGCTGCTG CCACGACCAC GATTAGTAAT AATTTAGAAG AAACTCCTGT TATTGAAAAT
    CATAGTTCCC AGCAACCCGT GAGTGAAGTT GCTACCGTCC CGTGTCCTAG AATTGATACT
    CAGCAACTGG ACCGGCAAAT TAAAGCAATT ATGAAAGAAG TCATTCCTTT TTTGAAGGAG
    CACATGGATG AAGTATGTTC TTCTCAACTT CTAACCTCAG TAAGACGCAT GGTTTTGGTC
    CTTACTCAGC AAAACAATGA GAGCAAAGAG TTTGTAAAGT TCTTTCATAA GCAGCTTGGA
    AGTATATTAC AGGATTCACT GGCAAAATTT GCTGGCAGAA AACTGAAGGA TTGTGGAGAA
    GATCTTCTTG TAGAGATATC TGAAGTGTTA TTTAATGAAT TGGCTTTCTT TAAGCTCATG
    CAAGATTTGG ATAATAATAG TGTAACTGTT AAACAGAGAT GCAAAAGGAA AATAGAAGCT
    GCTGGAGTGA TACAGTCTTA TGCAAAAGAG GCCAAAAGGA TTCTTGAAGG AGATCACAGT
    TCACCTGCTG GAGAAATTGA TGATGAAGAC AAAGACAAGG ATGAGACGGA AACAGTTAAG
    CAGACTCAAA CGTCTGAGGT TTATGATGGT GACGGCCCCA AAAATGTGAG ATCTGATATT
    TCTGATCAAG AGGATGATGA AGAAAGTGAA GAATGTCCAG TGTCTATTAG TTTGTCCAAA
    GCTGAAACTC AGGCTTTGAC TAATTACGGA AGTGGAGAAG ATGAGAATGA AGATGAAGAG
    ATAGAAGAAT TTGAAGAGGG ACCTGTAGAT GTCCAGACGT CACTTCAGGC TAACACTGAA
    ACTACAGAAG AAAATGAACA CGATGATCAG GTTCCACAGC ATGATTTTGA AAAATCATCA
    GAAAGCAAAA ATGTCCCATC AGAACAAGAA CCCACTACTA GTAAAGACTT TCTTTTGATG
    ACAGGTGACC AGGATAGCAC TCCTGTGAAG CCATGTTACC TCAATATCGT GGAAAATGAG
    CAACCTTTAA ATAGCCCTGT CCAGAAGGAC ACTCTCACTA CCATCGACTC GTCCAGTCAA
    CCTAACCCTT TGCCTTTACC TTTAACTGAA ATTGAAACCT TGGTGCCTAG AGTCAAAGAA
    GTGAAATCTG CTCAGGAGAC TCCTGAAAGT TCTTTGGCTG GAAGTCCTGA TACTGAATCT
    CCAGTGTTAG TGAATGACTA TGCAGATCTA AGGAAGAAAA TGGCCGAAGA AGAACAGAAA
    AACCACTTAT CTGGTGAAAT ATGTGCAATG CAGACTGAAG AATTAGCTGG GAATTCTCAG
    ACACTAAAAG AACCTGAAAC AGTGGGAGCC CAAAGTACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50989
    Clone ID Related Accession (Same CDS sequence) XM_010820283.2 , XM_010826087.2
    Accession Version XM_010820283.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC
    AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG
    TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT
    GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT
    GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT
    GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT
    ATGAAATTTC TCCAAAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA
    GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG
    TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG
    GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT
    CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA
    TGTAGCCGGA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATAGAA
    GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG
    CAGCTGAGAG CTCTACAGGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG
    CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT
    ACTGTACCAG GCAGACAGCC GGCTCGTTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA
    ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAATGAAGA ATTGAATGAT
    TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT
    AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCATCA
    GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA
    GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT
    TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA
    AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC
    AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT
    GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG
    AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA
    GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AGTCGGAATA TGATTCTGAG
    CATGAAAACT CTGAACCTGT TACTCATATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC
    GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCTAACA ATAGAGATGG GCGAGCAGTT
    AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT
    CCTCCTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA
    GGTCATGATG AGGAGGAGGA GGAGGAGGAA GCAGAAGATG AGGCCCTCAG TGGAGCTTCA
    CTGTCTAGTC ACAGCAGCAG CCTGATCATT GAGACTCCTG AAGATGCTGA ATTTGAACAT
    AAGATCCAGA AGCTTATGGC TGCAAAACTG AAACTTAGAC ACTTACAGAA CCTTGTAGCC
    ATGGTGCAGG ACGATGATGC AGCCAATCAA GGAGTTACTT CTGTGGAGGA TTTCTTCCCA
    GCAGAAGACA CCAAACAAAA TGCAAATAAC ACTAGAGGAA ATGCCAATAA AACACAAAAA
    GATGCTAGAG TAAATGAAAA GGCAAGAGAG AAATTTTATG AGGCTAAACT ACAGCAGCAA
    CAGAGAGAAC TCAAACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA
    ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC CTACAGCTGT CAGCTGCTAG TGTGGGTAAT
    TGTCCCACAA AAACACATAT GCCAGCTGTT ACTTCATCCC CAACCGTTAA TGAGAATGAA
    GCCAGTACAA ACAAATCTGG TTTTGAGCCT CATGATTCTT CAGTGGTAGA TAATGAGGTA
    TGGTCAGATA TGCGAAGACA TGAAATGTTA AGGGAGGAGC TACGACAGAG AAGAAAGCAG
    CTGGAAGCTC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TGGCTGAAAC TGCATCTCCA
    GTGGCTGTTT CACTGAGAAG TGATGGATCT GAGAACCTGT GTACACCTCA GCAAAGCAGA
    ACAGAAAAAA CAATGGCAAC TTGGGGAGGT TCTACCCAGT GTGCCCTAGA TGAAGAAGGG
    GATGAAGATG GTTACCTTTC TGAAGGAATT ATTCGGACAG ACGAAGAGGA GGAAGAGGAG
    GAGCAAGATG CCAGTTCCAA TGATAACTTT CATATGTATC CTCCAAACAG TGCAAATCGT
    AATTCTTACA ATGTAAAGGA AACTAAAAAT AGGTGGAAGA ACAATCGCCC TTTTTCAGCA
    GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAACAGA ATATCAGCAT GCAGCGTCAG
    GAAAACCTTC GCTGGGTGTC CGAACTCTCT TATGTAGAAG AGAAAGAACA ATGGCAAGAA
    CAAATTAATC AGCTAAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTAATG
    CAAGACCAGC AGACTCTGTC TTGTCTGCTA CAAACTCTTC TCACAAGTCC TTATAGTGTT
    ATGCCCAGCA GTGTTGCATC CCCGCAAGTG CACCTTATAA TGCACCAGTT GAACCAGTGC
    TATACCCAGC TAACGTGGCA GCAGAATAAT GTACAGAGGT TGAAACAAAT GCTCAGTGAA
    CTCATGCACC AACAAAATCA GCATCCAGAA AAACCTGCAA GCAAGGAAGG AGGCAGTGGT
    GCATCACACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA
    AATCTGTTTA ACCTACCGGG ATTTACTAAT TTTTCGTCAT TTGCACCAAG TATGAATTTC
    AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAAA ATATTTCTAC ACCCACTGAA
    CAGCAGCAAC CGTTAGCCCA GAATCCTTCA GGGAAAACAG AATACATGGC TTTTCCAAAA
    CCTTTTGAAA GTAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGAA
    GAGGAGGTGG AGAACAGTAG GACACCATGG TTTTATGACC AAGAAGGTGA GGTAGAAAAG
    CCATTTCTCA AGACTGGATT TGCAGTGTCT GTAGAGAAAA CAACAAATAG TAATCGCAAA
    AATCAACTAG ATACCAGTAG GAGAAGACGC CAGTTTGATG AAGTGTCCTT AGAAAGCTTT
    AACAGTATGC TTGACCCAGT AGATCCAACA ACAGTAACTA AAACATTCAA GTCAAGAAAA
    GCATCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAATCAAA GAGTAAGAAG
    AGGCATTCTG CTCAGCCGAA AAGCAGAGTG AAAAATATTG GGTATGAAAG TGCCAGTGTG
    TCTAGCACAT GTGAACCTTG CAAAAGTAGA AACAGACATT CAGCCCAGAC CGAAGAGCCT
    GTTCAAGCAA AAGTACTCAG CAGAAAGAAA CATGAGCAGC TGGAAAAAGT CATAAGATGT
    AGTAGGTCTA CAGAAATATC TTCAGCACAT GCTAGGAGAA TACTACAGCA GTCTAACAGA
    AATGCATGCA ATGAAGCGCC AGAAACCAGT AGTGATTTTT CCATGTTCGA AGCTTTGCGG
    GATACCATTT ATTCTGAAGT AGCTACGTTG ATTTCTCAAA ATGAATCTCG TCCACATTTT
    CTTATTGAAC TCTTCCATGA GCTTCAGCTA CTTAATACAG ACTATTTGAG ACAGAGGGCC
    TTATATGCAT TGCAGGACAT AGTATCCAGG CATATTTCTG AGAACCATGA AAAAGAAGGA
    GAAAATATAA AGTCCGTGAA TTCTGGTACT TGGATAGCAT CCAACTCAGA ACTTACTCCC
    AGTGAAAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA
    CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TATCTTCAAC TTGTGAGCCT
    TTTGCAACAG ATGTTCTGGG TGACACTGTG ATTCACTTAG ATCAGGCATT AGCCAGAATG
    AGAGACTACA CGCATATGAA GATGGAGGCT GAAAGTAGTC CAGACGTCAG GTGCACCTGC
    AGGATCGTTG AGGATGACGA TGGTGCTGCT GCCACGACCA CGATTAGTAA TAATTTAGAA
    GAAACTCCTG TTATTGAAAA TCATAGTTCC CAGCAACCCG TGAGTGAAGT TGCTACCGTC
    CCGTGTCCTA GAATTGATAC TCAGCAACTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA
    GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGTT CTTCTCAACT TCTAACCTCA
    GTAAGACGCA TGGTTTTGGT CCTTACTCAG CAAAACAATG AGAGCAAAGA GTTTGTAAAG
    TTCTTTCATA AGCAGCTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA
    AAACTGAAGG ATTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT ATTTAATGAA
    TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTGTAACTGT TAAACAGAGA
    TGCAAAAGGA AAATAGAAGC TGCTGGAGTG ATACAGTCTT ATGCAAAAGA GGCCAAAAGG
    ATTCTTGAAG GAGATCACAG TTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG
    GATGAGACGG AAACAGTTAA GCAGACTCAA ACGTCTGAGG TTTATGATGG TGACGGCCCC
    AAAAATGTGA GATCTGATAT TTCTGATCAA GAGGATGATG AAGAAAGTGA AGAATGTCCA
    GTGTCTATTA GTTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTACGG AAGTGGAGAA
    GATGAGAATG AAGATGAAGA GATAGAAGAA TTTGAAGAGG GACCTGTAGA TGTCCAGACG
    TCACTTCAGG CTAACACTGA AACTACAGAA GAAAATGAAC ACGATGATCA GGTTCCACAG
    CATGATTTTG AAAAATCATC AGAAAGCAAA AATGTCCCAT CAGAACAAGA ACCCACTACT
    AGTAAAGACT TTCTTTTGAT GACAGGTGAC CAGGATAGCA CTCCTGTGAA GCCATGTTAC
    CTCAATATCG TGGAAAATGA GCAACCTTTA AATAGCCCTG TCCAGAAGGA CACTCTCACT
    ACCATCGACT CGTCCAGTCA ACCTAACCCT TTGCCTTTAC CTTTAACTGA AATTGAAACC
    TTGGTGCCTA GAGTCAAAGA AGTGAAATCT GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT
    GGAAGTCCTG ATACTGAATC TCCAGTGTTA GTGAATGACT ATGAAGCAGA ATCTGGTAAC
    ATAAGTCAAA AATCTGATGA AGAAGACTTT GTGAAAGTTG AAGATTTACC ACTTAAACTG
    ACAATATATT CAGAGGCAGA TCTAAGGAAG AAAATGGCCG AAGAAGAACA GAAAAACCAC
    TTATCTGGTG AAATATGTGC AATGCAGACT GAAGAATTAG CTGGGAATTC TCAGACACTA
    AAAGAACCTG AAACAGTGGG AGCCCAAAGT ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818585.1
    CDS465..6920
    Translation

    Target ORF information:

    RefSeq Version XM_010820283.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820283.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC
    AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG
    TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT
    GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT
    GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT
    GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT
    ATGAAATTTC TCCAAAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA
    GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG
    TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG
    GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT
    CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA
    TGTAGCCGGA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATAGAA
    GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG
    CAGCTGAGAG CTCTACAGGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG
    CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT
    ACTGTACCAG GCAGACAGCC GGCTCGTTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA
    ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAATGAAGA ATTGAATGAT
    TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT
    AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCATCA
    GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA
    GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT
    TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA
    AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC
    AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT
    GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG
    AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA
    GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AGTCGGAATA TGATTCTGAG
    CATGAAAACT CTGAACCTGT TACTCATATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC
    GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCTAACA ATAGAGATGG GCGAGCAGTT
    AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT
    CCTCCTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA
    GGTCATGATG AGGAGGAGGA GGAGGAGGAA GCAGAAGATG AGGCCCTCAG TGGAGCTTCA
    CTGTCTAGTC ACAGCAGCAG CCTGATCATT GAGACTCCTG AAGATGCTGA ATTTGAACAT
    AAGATCCAGA AGCTTATGGC TGCAAAACTG AAACTTAGAC ACTTACAGAA CCTTGTAGCC
    ATGGTGCAGG ACGATGATGC AGCCAATCAA GGAGTTACTT CTGTGGAGGA TTTCTTCCCA
    GCAGAAGACA CCAAACAAAA TGCAAATAAC ACTAGAGGAA ATGCCAATAA AACACAAAAA
    GATGCTAGAG TAAATGAAAA GGCAAGAGAG AAATTTTATG AGGCTAAACT ACAGCAGCAA
    CAGAGAGAAC TCAAACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA
    ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC CTACAGCTGT CAGCTGCTAG TGTGGGTAAT
    TGTCCCACAA AAACACATAT GCCAGCTGTT ACTTCATCCC CAACCGTTAA TGAGAATGAA
    GCCAGTACAA ACAAATCTGG TTTTGAGCCT CATGATTCTT CAGTGGTAGA TAATGAGGTA
    TGGTCAGATA TGCGAAGACA TGAAATGTTA AGGGAGGAGC TACGACAGAG AAGAAAGCAG
    CTGGAAGCTC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TGGCTGAAAC TGCATCTCCA
    GTGGCTGTTT CACTGAGAAG TGATGGATCT GAGAACCTGT GTACACCTCA GCAAAGCAGA
    ACAGAAAAAA CAATGGCAAC TTGGGGAGGT TCTACCCAGT GTGCCCTAGA TGAAGAAGGG
    GATGAAGATG GTTACCTTTC TGAAGGAATT ATTCGGACAG ACGAAGAGGA GGAAGAGGAG
    GAGCAAGATG CCAGTTCCAA TGATAACTTT CATATGTATC CTCCAAACAG TGCAAATCGT
    AATTCTTACA ATGTAAAGGA AACTAAAAAT AGGTGGAAGA ACAATCGCCC TTTTTCAGCA
    GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAACAGA ATATCAGCAT GCAGCGTCAG
    GAAAACCTTC GCTGGGTGTC CGAACTCTCT TATGTAGAAG AGAAAGAACA ATGGCAAGAA
    CAAATTAATC AGCTAAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTAATG
    CAAGACCAGC AGACTCTGTC TTGTCTGCTA CAAACTCTTC TCACAAGTCC TTATAGTGTT
    ATGCCCAGCA GTGTTGCATC CCCGCAAGTG CACCTTATAA TGCACCAGTT GAACCAGTGC
    TATACCCAGC TAACGTGGCA GCAGAATAAT GTACAGAGGT TGAAACAAAT GCTCAGTGAA
    CTCATGCACC AACAAAATCA GCATCCAGAA AAACCTGCAA GCAAGGAAGG AGGCAGTGGT
    GCATCACACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA
    AATCTGTTTA ACCTACCGGG ATTTACTAAT TTTTCGTCAT TTGCACCAAG TATGAATTTC
    AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAAA ATATTTCTAC ACCCACTGAA
    CAGCAGCAAC CGTTAGCCCA GAATCCTTCA GGGAAAACAG AATACATGGC TTTTCCAAAA
    CCTTTTGAAA GTAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGAA
    GAGGAGGTGG AGAACAGTAG GACACCATGG TTTTATGACC AAGAAGGTGA GGTAGAAAAG
    CCATTTCTCA AGACTGGATT TGCAGTGTCT GTAGAGAAAA CAACAAATAG TAATCGCAAA
    AATCAACTAG ATACCAGTAG GAGAAGACGC CAGTTTGATG AAGTGTCCTT AGAAAGCTTT
    AACAGTATGC TTGACCCAGT AGATCCAACA ACAGTAACTA AAACATTCAA GTCAAGAAAA
    GCATCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAATCAAA GAGTAAGAAG
    AGGCATTCTG CTCAGCCGAA AAGCAGAGTG AAAAATATTG GGTATGAAAG TGCCAGTGTG
    TCTAGCACAT GTGAACCTTG CAAAAGTAGA AACAGACATT CAGCCCAGAC CGAAGAGCCT
    GTTCAAGCAA AAGTACTCAG CAGAAAGAAA CATGAGCAGC TGGAAAAAGT CATAAGATGT
    AGTAGGTCTA CAGAAATATC TTCAGCACAT GCTAGGAGAA TACTACAGCA GTCTAACAGA
    AATGCATGCA ATGAAGCGCC AGAAACCAGT AGTGATTTTT CCATGTTCGA AGCTTTGCGG
    GATACCATTT ATTCTGAAGT AGCTACGTTG ATTTCTCAAA ATGAATCTCG TCCACATTTT
    CTTATTGAAC TCTTCCATGA GCTTCAGCTA CTTAATACAG ACTATTTGAG ACAGAGGGCC
    TTATATGCAT TGCAGGACAT AGTATCCAGG CATATTTCTG AGAACCATGA AAAAGAAGGA
    GAAAATATAA AGTCCGTGAA TTCTGGTACT TGGATAGCAT CCAACTCAGA ACTTACTCCC
    AGTGAAAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA
    CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TATCTTCAAC TTGTGAGCCT
    TTTGCAACAG ATGTTCTGGG TGACACTGTG ATTCACTTAG ATCAGGCATT AGCCAGAATG
    AGAGACTACA CGCATATGAA GATGGAGGCT GAAAGTAGTC CAGACGTCAG GTGCACCTGC
    AGGATCGTTG AGGATGACGA TGGTGCTGCT GCCACGACCA CGATTAGTAA TAATTTAGAA
    GAAACTCCTG TTATTGAAAA TCATAGTTCC CAGCAACCCG TGAGTGAAGT TGCTACCGTC
    CCGTGTCCTA GAATTGATAC TCAGCAACTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA
    GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGTT CTTCTCAACT TCTAACCTCA
    GTAAGACGCA TGGTTTTGGT CCTTACTCAG CAAAACAATG AGAGCAAAGA GTTTGTAAAG
    TTCTTTCATA AGCAGCTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA
    AAACTGAAGG ATTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT ATTTAATGAA
    TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTGTAACTGT TAAACAGAGA
    TGCAAAAGGA AAATAGAAGC TGCTGGAGTG ATACAGTCTT ATGCAAAAGA GGCCAAAAGG
    ATTCTTGAAG GAGATCACAG TTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG
    GATGAGACGG AAACAGTTAA GCAGACTCAA ACGTCTGAGG TTTATGATGG TGACGGCCCC
    AAAAATGTGA GATCTGATAT TTCTGATCAA GAGGATGATG AAGAAAGTGA AGAATGTCCA
    GTGTCTATTA GTTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTACGG AAGTGGAGAA
    GATGAGAATG AAGATGAAGA GATAGAAGAA TTTGAAGAGG GACCTGTAGA TGTCCAGACG
    TCACTTCAGG CTAACACTGA AACTACAGAA GAAAATGAAC ACGATGATCA GGTTCCACAG
    CATGATTTTG AAAAATCATC AGAAAGCAAA AATGTCCCAT CAGAACAAGA ACCCACTACT
    AGTAAAGACT TTCTTTTGAT GACAGGTGAC CAGGATAGCA CTCCTGTGAA GCCATGTTAC
    CTCAATATCG TGGAAAATGA GCAACCTTTA AATAGCCCTG TCCAGAAGGA CACTCTCACT
    ACCATCGACT CGTCCAGTCA ACCTAACCCT TTGCCTTTAC CTTTAACTGA AATTGAAACC
    TTGGTGCCTA GAGTCAAAGA AGTGAAATCT GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT
    GGAAGTCCTG ATACTGAATC TCCAGTGTTA GTGAATGACT ATGAAGCAGA ATCTGGTAAC
    ATAAGTCAAA AATCTGATGA AGAAGACTTT GTGAAAGTTG AAGATTTACC ACTTAAACTG
    ACAATATATT CAGAGGCAGA TCTAAGGAAG AAAATGGCCG AAGAAGAACA GAAAAACCAC
    TTATCTGGTG AAATATGTGC AATGCAGACT GAAGAATTAG CTGGGAATTC TCAGACACTA
    AAAGAACCTG AAACAGTGGG AGCCCAAAGT ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50989
    Clone ID Related Accession (Same CDS sequence) XM_010820283.2 , XM_010826087.2
    Accession Version XM_010826087.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646718.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826087.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC
    AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG
    TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT
    GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT
    GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT
    GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT
    ATGAAATTTC TCCAAAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA
    GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG
    TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG
    GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT
    CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA
    TGTAGCCGGA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATAGAA
    GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG
    CAGCTGAGAG CTCTACAGGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG
    CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT
    ACTGTACCAG GCAGACAGCC GGCTCGTTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA
    ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAATGAAGA ATTGAATGAT
    TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT
    AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCATCA
    GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA
    GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT
    TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA
    AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC
    AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT
    GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG
    AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA
    GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AGTCGGAATA TGATTCTGAG
    CATGAAAACT CTGAACCTGT TACTCATATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC
    GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCTAACA ATAGAGATGG GCGAGCAGTT
    AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT
    CCTCCTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA
    GGTCATGATG AGGAGGAGGA GGAGGAGGAA GCAGAAGATG AGGCCCTCAG TGGAGCTTCA
    CTGTCTAGTC ACAGCAGCAG CCTGATCATT GAGACTCCTG AAGATGCTGA ATTTGAACAT
    AAGATCCAGA AGCTTATGGC TGCAAAACTG AAACTTAGAC ACTTACAGAA CCTTGTAGCC
    ATGGTGCAGG ACGATGATGC AGCCAATCAA GGAGTTACTT CTGTGGAGGA TTTCTTCCCA
    GCAGAAGACA CCAAACAAAA TGCAAATAAC ACTAGAGGAA ATGCCAATAA AACACAAAAA
    GATGCTAGAG TAAATGAAAA GGCAAGAGAG AAATTTTATG AGGCTAAACT ACAGCAGCAA
    CAGAGAGAAC TCAAACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA
    ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC CTACAGCTGT CAGCTGCTAG TGTGGGTAAT
    TGTCCCACAA AAACACATAT GCCAGCTGTT ACTTCATCCC CAACCGTTAA TGAGAATGAA
    GCCAGTACAA ACAAATCTGG TTTTGAGCCT CATGATTCTT CAGTGGTAGA TAATGAGGTA
    TGGTCAGATA TGCGAAGACA TGAAATGTTA AGGGAGGAGC TACGACAGAG AAGAAAGCAG
    CTGGAAGCTC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TGGCTGAAAC TGCATCTCCA
    GTGGCTGTTT CACTGAGAAG TGATGGATCT GAGAACCTGT GTACACCTCA GCAAAGCAGA
    ACAGAAAAAA CAATGGCAAC TTGGGGAGGT TCTACCCAGT GTGCCCTAGA TGAAGAAGGG
    GATGAAGATG GTTACCTTTC TGAAGGAATT ATTCGGACAG ACGAAGAGGA GGAAGAGGAG
    GAGCAAGATG CCAGTTCCAA TGATAACTTT CATATGTATC CTCCAAACAG TGCAAATCGT
    AATTCTTACA ATGTAAAGGA AACTAAAAAT AGGTGGAAGA ACAATCGCCC TTTTTCAGCA
    GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAACAGA ATATCAGCAT GCAGCGTCAG
    GAAAACCTTC GCTGGGTGTC CGAACTCTCT TATGTAGAAG AGAAAGAACA ATGGCAAGAA
    CAAATTAATC AGCTAAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTAATG
    CAAGACCAGC AGACTCTGTC TTGTCTGCTA CAAACTCTTC TCACAAGTCC TTATAGTGTT
    ATGCCCAGCA GTGTTGCATC CCCGCAAGTG CACCTTATAA TGCACCAGTT GAACCAGTGC
    TATACCCAGC TAACGTGGCA GCAGAATAAT GTACAGAGGT TGAAACAAAT GCTCAGTGAA
    CTCATGCACC AACAAAATCA GCATCCAGAA AAACCTGCAA GCAAGGAAGG AGGCAGTGGT
    GCATCACACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA
    AATCTGTTTA ACCTACCGGG ATTTACTAAT TTTTCGTCAT TTGCACCAAG TATGAATTTC
    AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAAA ATATTTCTAC ACCCACTGAA
    CAGCAGCAAC CGTTAGCCCA GAATCCTTCA GGGAAAACAG AATACATGGC TTTTCCAAAA
    CCTTTTGAAA GTAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGAA
    GAGGAGGTGG AGAACAGTAG GACACCATGG TTTTATGACC AAGAAGGTGA GGTAGAAAAG
    CCATTTCTCA AGACTGGATT TGCAGTGTCT GTAGAGAAAA CAACAAATAG TAATCGCAAA
    AATCAACTAG ATACCAGTAG GAGAAGACGC CAGTTTGATG AAGTGTCCTT AGAAAGCTTT
    AACAGTATGC TTGACCCAGT AGATCCAACA ACAGTAACTA AAACATTCAA GTCAAGAAAA
    GCATCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAATCAAA GAGTAAGAAG
    AGGCATTCTG CTCAGCCGAA AAGCAGAGTG AAAAATATTG GGTATGAAAG TGCCAGTGTG
    TCTAGCACAT GTGAACCTTG CAAAAGTAGA AACAGACATT CAGCCCAGAC CGAAGAGCCT
    GTTCAAGCAA AAGTACTCAG CAGAAAGAAA CATGAGCAGC TGGAAAAAGT CATAAGATGT
    AGTAGGTCTA CAGAAATATC TTCAGCACAT GCTAGGAGAA TACTACAGCA GTCTAACAGA
    AATGCATGCA ATGAAGCGCC AGAAACCAGT AGTGATTTTT CCATGTTCGA AGCTTTGCGG
    GATACCATTT ATTCTGAAGT AGCTACGTTG ATTTCTCAAA ATGAATCTCG TCCACATTTT
    CTTATTGAAC TCTTCCATGA GCTTCAGCTA CTTAATACAG ACTATTTGAG ACAGAGGGCC
    TTATATGCAT TGCAGGACAT AGTATCCAGG CATATTTCTG AGAACCATGA AAAAGAAGGA
    GAAAATATAA AGTCCGTGAA TTCTGGTACT TGGATAGCAT CCAACTCAGA ACTTACTCCC
    AGTGAAAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA
    CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TATCTTCAAC TTGTGAGCCT
    TTTGCAACAG ATGTTCTGGG TGACACTGTG ATTCACTTAG ATCAGGCATT AGCCAGAATG
    AGAGACTACA CGCATATGAA GATGGAGGCT GAAAGTAGTC CAGACGTCAG GTGCACCTGC
    AGGATCGTTG AGGATGACGA TGGTGCTGCT GCCACGACCA CGATTAGTAA TAATTTAGAA
    GAAACTCCTG TTATTGAAAA TCATAGTTCC CAGCAACCCG TGAGTGAAGT TGCTACCGTC
    CCGTGTCCTA GAATTGATAC TCAGCAACTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA
    GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGTT CTTCTCAACT TCTAACCTCA
    GTAAGACGCA TGGTTTTGGT CCTTACTCAG CAAAACAATG AGAGCAAAGA GTTTGTAAAG
    TTCTTTCATA AGCAGCTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA
    AAACTGAAGG ATTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT ATTTAATGAA
    TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTGTAACTGT TAAACAGAGA
    TGCAAAAGGA AAATAGAAGC TGCTGGAGTG ATACAGTCTT ATGCAAAAGA GGCCAAAAGG
    ATTCTTGAAG GAGATCACAG TTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG
    GATGAGACGG AAACAGTTAA GCAGACTCAA ACGTCTGAGG TTTATGATGG TGACGGCCCC
    AAAAATGTGA GATCTGATAT TTCTGATCAA GAGGATGATG AAGAAAGTGA AGAATGTCCA
    GTGTCTATTA GTTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTACGG AAGTGGAGAA
    GATGAGAATG AAGATGAAGA GATAGAAGAA TTTGAAGAGG GACCTGTAGA TGTCCAGACG
    TCACTTCAGG CTAACACTGA AACTACAGAA GAAAATGAAC ACGATGATCA GGTTCCACAG
    CATGATTTTG AAAAATCATC AGAAAGCAAA AATGTCCCAT CAGAACAAGA ACCCACTACT
    AGTAAAGACT TTCTTTTGAT GACAGGTGAC CAGGATAGCA CTCCTGTGAA GCCATGTTAC
    CTCAATATCG TGGAAAATGA GCAACCTTTA AATAGCCCTG TCCAGAAGGA CACTCTCACT
    ACCATCGACT CGTCCAGTCA ACCTAACCCT TTGCCTTTAC CTTTAACTGA AATTGAAACC
    TTGGTGCCTA GAGTCAAAGA AGTGAAATCT GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT
    GGAAGTCCTG ATACTGAATC TCCAGTGTTA GTGAATGACT ATGAAGCAGA ATCTGGTAAC
    ATAAGTCAAA AATCTGATGA AGAAGACTTT GTGAAAGTTG AAGATTTACC ACTTAAACTG
    ACAATATATT CAGAGGCAGA TCTAAGGAAG AAAATGGCCG AAGAAGAACA GAAAAACCAC
    TTATCTGGTG AAATATGTGC AATGCAGACT GAAGAATTAG CTGGGAATTC TCAGACACTA
    AAAGAACCTG AAACAGTGGG AGCCCAAAGT ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824389.1
    CDS465..6920
    Translation

    Target ORF information:

    RefSeq Version XM_010826087.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826087.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGATTAATAC TAACAAGAGC
    AAAGATGCCA CTGTAAGTCC TCAAAAGAGA GAAATGATTG GATCAGCACA GTGTAAAGAG
    TTGTTTGCTT CTGCTTTAAG TAATGACCTT TTGCAAAACT GTCAAGTCTC TGAAGAAGAT
    GGAAGAGGAG AACCTGCAAT GGAGAGCAGC CAGATTGTAA GCAGGCTTGT TCAGATTCGT
    GAGTATATTA CTAAAGCTAG TTCCATGCGG GAAGACCTTG TAGAGAAAAA TGAAAGATCT
    GCTAATGTTG AGCGCCTCAC TCATCTAATA GATCACCTTA AAGAACAAGA AAAGTCATAT
    ATGAAATTTC TCCAAAAGAT TCTTGCTAGA GAAAATGAGG AGGAGGATGT TCGGACTATA
    GATTCAGCTG TGGGATCTGG TTCTGTAGCT GAGAGCACAT CGCTAAACAT AGATGTGCGG
    TCTGAGGCTT CAGATGCCAC GGAGGCATCT TTTAGTCTGA GAATTCGGCC GTGCGTTGAG
    GACAAACTAG GGAATTCAGC TTCACAAGCA CAGGTTTCAG ACACTGACGT TACTACAAGT
    CCAAAAGGGA AAGGTGACAG ACCTCCTCAG CATGACAGGG AGCTGAGGCC TAATAGGAAA
    TGTAGCCGGA AACGTGGATT TCCCTCAAAG GCCAGAGATC CTCAGCAGGA GCCTATAGAA
    GAGATAGAAA ATTTGAAGAA ACAACATGAT TTATTAAAAA GGATGTTACA GCAGCAGGAG
    CAGCTGAGAG CTCTACAGGG ACGACAAGCT GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG
    CAGGCTATAG CTGTGATGGA TGATTCTGAA AAGGTTGCGG AGACAACTCC TGGCCATCAT
    ACTGTACCAG GCAGACAGCC GGCTCGTTCA CCTTTGCATC AGAGAGTACC CATAAGAGAA
    ACTACAGGTA GCCTATCTGG AGTCAGTATC ACCTCTGAAC TAAATGAAGA ATTGAATGAT
    TTAATTCAGC ATTTTCATAA TCAGCTTCGT GATTCTCAGC CTCCAGCTGT TCCAGACAAT
    AGAAGACAAG CAGAAAGTCT TTCATTAACT AGGGAGATTT CTCAGAGCAG AAATCCATCA
    GTTTCAGAGC ATTTACCTGA TGAGAAAGTC CAACTTTTTA GCAAGATGAG AGTGTTACAA
    GAAAAGAAAC AAAAAATGGA CAAACTTCTT GGAGAACTTC ATACACTTCG AGATCAGCAT
    TTGAACAATT CATCATTTGT GCCTTCTTCA GCCTCTCCAC AAAGGAGTGT GGATCAGAGA
    AGTACAACCA CAGCTCCTTC TGCTCCTGTA GTCTTAACAC CAGTGGTCAA TGGAGAATCC
    AATAACCTCA CGCCATCTGT TCCTTATCCT GCTGCTTCTC TAGTTTCTCA GAATCAGAAT
    GAAAATGAAG GCCACCTAAA TCCAACTGAA AAACTCCAGA AGTTAAATGA AGTTCGAAAG
    AGGTTGAATG AACTAAGAGA ATTAGTTCAT TATTATGAGC AAACATCAGA TATGATGACA
    GATGCTGTGA ATGAAAACAC AAAAGAAGAA GAAACTGAAG AGTCGGAATA TGATTCTGAG
    CATGAAAACT CTGAACCTGT TACTCATATT CGAAATCCAC AAGTGGCTGC CACCTGGAAC
    GAAGTAAATA GTAATTCTAA TGCACAGTGT GTTTCTAACA ATAGAGATGG GCGAGCAGTT
    AATTCTAACT GTGAAATTAA CAACAGGTCT GCTGCCAACA TAAGGGCTCT AAACATGCCT
    CCTCCTTCAG CAGATTGTCG ATACAATAGA GAAGGAGAAC AGGAGATGCC TGTTGCACAA
    GGTCATGATG AGGAGGAGGA GGAGGAGGAA GCAGAAGATG AGGCCCTCAG TGGAGCTTCA
    CTGTCTAGTC ACAGCAGCAG CCTGATCATT GAGACTCCTG AAGATGCTGA ATTTGAACAT
    AAGATCCAGA AGCTTATGGC TGCAAAACTG AAACTTAGAC ACTTACAGAA CCTTGTAGCC
    ATGGTGCAGG ACGATGATGC AGCCAATCAA GGAGTTACTT CTGTGGAGGA TTTCTTCCCA
    GCAGAAGACA CCAAACAAAA TGCAAATAAC ACTAGAGGAA ATGCCAATAA AACACAAAAA
    GATGCTAGAG TAAATGAAAA GGCAAGAGAG AAATTTTATG AGGCTAAACT ACAGCAGCAA
    CAGAGAGAAC TCAAACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA
    ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC CTACAGCTGT CAGCTGCTAG TGTGGGTAAT
    TGTCCCACAA AAACACATAT GCCAGCTGTT ACTTCATCCC CAACCGTTAA TGAGAATGAA
    GCCAGTACAA ACAAATCTGG TTTTGAGCCT CATGATTCTT CAGTGGTAGA TAATGAGGTA
    TGGTCAGATA TGCGAAGACA TGAAATGTTA AGGGAGGAGC TACGACAGAG AAGAAAGCAG
    CTGGAAGCTC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TGGCTGAAAC TGCATCTCCA
    GTGGCTGTTT CACTGAGAAG TGATGGATCT GAGAACCTGT GTACACCTCA GCAAAGCAGA
    ACAGAAAAAA CAATGGCAAC TTGGGGAGGT TCTACCCAGT GTGCCCTAGA TGAAGAAGGG
    GATGAAGATG GTTACCTTTC TGAAGGAATT ATTCGGACAG ACGAAGAGGA GGAAGAGGAG
    GAGCAAGATG CCAGTTCCAA TGATAACTTT CATATGTATC CTCCAAACAG TGCAAATCGT
    AATTCTTACA ATGTAAAGGA AACTAAAAAT AGGTGGAAGA ACAATCGCCC TTTTTCAGCA
    GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAACAGA ATATCAGCAT GCAGCGTCAG
    GAAAACCTTC GCTGGGTGTC CGAACTCTCT TATGTAGAAG AGAAAGAACA ATGGCAAGAA
    CAAATTAATC AGCTAAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTAATG
    CAAGACCAGC AGACTCTGTC TTGTCTGCTA CAAACTCTTC TCACAAGTCC TTATAGTGTT
    ATGCCCAGCA GTGTTGCATC CCCGCAAGTG CACCTTATAA TGCACCAGTT GAACCAGTGC
    TATACCCAGC TAACGTGGCA GCAGAATAAT GTACAGAGGT TGAAACAAAT GCTCAGTGAA
    CTCATGCACC AACAAAATCA GCATCCAGAA AAACCTGCAA GCAAGGAAGG AGGCAGTGGT
    GCATCACACC CTCCTTCTCC CAGTTTATTT TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA
    AATCTGTTTA ACCTACCGGG ATTTACTAAT TTTTCGTCAT TTGCACCAAG TATGAATTTC
    AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT TTTTCTCAAA ATATTTCTAC ACCCACTGAA
    CAGCAGCAAC CGTTAGCCCA GAATCCTTCA GGGAAAACAG AATACATGGC TTTTCCAAAA
    CCTTTTGAAA GTAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGAA
    GAGGAGGTGG AGAACAGTAG GACACCATGG TTTTATGACC AAGAAGGTGA GGTAGAAAAG
    CCATTTCTCA AGACTGGATT TGCAGTGTCT GTAGAGAAAA CAACAAATAG TAATCGCAAA
    AATCAACTAG ATACCAGTAG GAGAAGACGC CAGTTTGATG AAGTGTCCTT AGAAAGCTTT
    AACAGTATGC TTGACCCAGT AGATCCAACA ACAGTAACTA AAACATTCAA GTCAAGAAAA
    GCATCTGCAC AGGCCAGCCT GGCATCTAAA GATAAAACTC CCAAATCAAA GAGTAAGAAG
    AGGCATTCTG CTCAGCCGAA AAGCAGAGTG AAAAATATTG GGTATGAAAG TGCCAGTGTG
    TCTAGCACAT GTGAACCTTG CAAAAGTAGA AACAGACATT CAGCCCAGAC CGAAGAGCCT
    GTTCAAGCAA AAGTACTCAG CAGAAAGAAA CATGAGCAGC TGGAAAAAGT CATAAGATGT
    AGTAGGTCTA CAGAAATATC TTCAGCACAT GCTAGGAGAA TACTACAGCA GTCTAACAGA
    AATGCATGCA ATGAAGCGCC AGAAACCAGT AGTGATTTTT CCATGTTCGA AGCTTTGCGG
    GATACCATTT ATTCTGAAGT AGCTACGTTG ATTTCTCAAA ATGAATCTCG TCCACATTTT
    CTTATTGAAC TCTTCCATGA GCTTCAGCTA CTTAATACAG ACTATTTGAG ACAGAGGGCC
    TTATATGCAT TGCAGGACAT AGTATCCAGG CATATTTCTG AGAACCATGA AAAAGAAGGA
    GAAAATATAA AGTCCGTGAA TTCTGGTACT TGGATAGCAT CCAACTCAGA ACTTACTCCC
    AGTGAAAGCC TTGCTACTAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA
    CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGTG TATCTTCAAC TTGTGAGCCT
    TTTGCAACAG ATGTTCTGGG TGACACTGTG ATTCACTTAG ATCAGGCATT AGCCAGAATG
    AGAGACTACA CGCATATGAA GATGGAGGCT GAAAGTAGTC CAGACGTCAG GTGCACCTGC
    AGGATCGTTG AGGATGACGA TGGTGCTGCT GCCACGACCA CGATTAGTAA TAATTTAGAA
    GAAACTCCTG TTATTGAAAA TCATAGTTCC CAGCAACCCG TGAGTGAAGT TGCTACCGTC
    CCGTGTCCTA GAATTGATAC TCAGCAACTG GACCGGCAAA TTAAAGCAAT TATGAAAGAA
    GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGTT CTTCTCAACT TCTAACCTCA
    GTAAGACGCA TGGTTTTGGT CCTTACTCAG CAAAACAATG AGAGCAAAGA GTTTGTAAAG
    TTCTTTCATA AGCAGCTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA
    AAACTGAAGG ATTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT ATTTAATGAA
    TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTGTAACTGT TAAACAGAGA
    TGCAAAAGGA AAATAGAAGC TGCTGGAGTG ATACAGTCTT ATGCAAAAGA GGCCAAAAGG
    ATTCTTGAAG GAGATCACAG TTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG
    GATGAGACGG AAACAGTTAA GCAGACTCAA ACGTCTGAGG TTTATGATGG TGACGGCCCC
    AAAAATGTGA GATCTGATAT TTCTGATCAA GAGGATGATG AAGAAAGTGA AGAATGTCCA
    GTGTCTATTA GTTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTACGG AAGTGGAGAA
    GATGAGAATG AAGATGAAGA GATAGAAGAA TTTGAAGAGG GACCTGTAGA TGTCCAGACG
    TCACTTCAGG CTAACACTGA AACTACAGAA GAAAATGAAC ACGATGATCA GGTTCCACAG
    CATGATTTTG AAAAATCATC AGAAAGCAAA AATGTCCCAT CAGAACAAGA ACCCACTACT
    AGTAAAGACT TTCTTTTGAT GACAGGTGAC CAGGATAGCA CTCCTGTGAA GCCATGTTAC
    CTCAATATCG TGGAAAATGA GCAACCTTTA AATAGCCCTG TCCAGAAGGA CACTCTCACT
    ACCATCGACT CGTCCAGTCA ACCTAACCCT TTGCCTTTAC CTTTAACTGA AATTGAAACC
    TTGGTGCCTA GAGTCAAAGA AGTGAAATCT GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT
    GGAAGTCCTG ATACTGAATC TCCAGTGTTA GTGAATGACT ATGAAGCAGA ATCTGGTAAC
    ATAAGTCAAA AATCTGATGA AGAAGACTTT GTGAAAGTTG AAGATTTACC ACTTAAACTG
    ACAATATATT CAGAGGCAGA TCTAAGGAAG AAAATGGCCG AAGAAGAACA GAAAAACCAC
    TTATCTGGTG AAATATGTGC AATGCAGACT GAAGAATTAG CTGGGAATTC TCAGACACTA
    AAAGAACCTG AAACAGTGGG AGCCCAAAGT ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50990
    Clone ID Related Accession (Same CDS sequence) XM_010820284.2 , XM_010826088.2
    Accession Version XM_010820284.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820284.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG CAGATTGTCG ATACAATAGA
    GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGATG AGGAGGAGGA GGAGGAGGAA
    GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT
    GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG
    AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA
    GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC
    ACTAGAGGAA ATGCCAATAA AACACAAAAA GATGCTAGAG TAAATGAAAA GGCAAGAGAG
    AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA
    AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC
    CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT
    ACTTCATCCC CAACCGTTAA TGAGAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT
    CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA
    AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG
    AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT
    GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT
    TCTACCCAGT GTGCCCTAGA TGAAGAAGGG GATGAAGATG GTTACCTTTC TGAAGGAATT
    ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT
    CATATGTATC CTCCAAACAG TGCAAATCGT AATTCTTACA ATGTAAAGGA AACTAAAAAT
    AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA
    AGACAACAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT
    TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT
    TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA
    CAAACTCTTC TCACAAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG
    CACCTTATAA TGCACCAGTT GAACCAGTGC TATACCCAGC TAACGTGGCA GCAGAATAAT
    GTACAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCACC AACAAAATCA GCATCCAGAA
    AAACCTGCAA GCAAGGAAGG AGGCAGTGGT GCATCACACC CTCCTTCTCC CAGTTTATTT
    TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG ATTTACTAAT
    TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT
    TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA
    GGGAAAACAG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCA
    GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG
    TTTTATGACC AAGAAGGTGA GGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT
    GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC
    CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA
    ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA
    GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTG CTCAGCCGAA AAGCAGAGTG
    AAAAATATTG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA
    AACAGACATT CAGCCCAGAC CGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAA
    CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGCACAT
    GCTAGGAGAA TACTACAGCA GTCTAACAGA AATGCATGCA ATGAAGCGCC AGAAACCAGT
    AGTGATTTTT CCATGTTCGA AGCTTTGCGG GATACCATTT ATTCTGAAGT AGCTACGTTG
    ATTTCTCAAA ATGAATCTCG TCCACATTTT CTTATTGAAC TCTTCCATGA GCTTCAGCTA
    CTTAATACAG ACTATTTGAG ACAGAGGGCC TTATATGCAT TGCAGGACAT AGTATCCAGG
    CATATTTCTG AGAACCATGA AAAAGAAGGA GAAAATATAA AGTCCGTGAA TTCTGGTACT
    TGGATAGCAT CCAACTCAGA ACTTACTCCC AGTGAAAGCC TTGCTACTAC TGATGATGAA
    ACTTTTGAGA AGAACTTTGA GAGAGAAACA CATAAAATAA GTGAGCAAAA TGATGCTGAT
    AATGCTAGTG TATCTTCAAC TTGTGAGCCT TTTGCAACAG ATGTTCTGGG TGACACTGTG
    ATTCACTTAG ATCAGGCATT AGCCAGAATG AGAGACTACA CGCATATGAA GATGGAGGCT
    GAAAGTAGTC CAGACGTCAG GTGCACCTGC AGGATCGTTG AGGATGACGA TGGTGCTGCT
    GCCACGACCA CGATTAGTAA TAATTTAGAA GAAACTCCTG TTATTGAAAA TCATAGTTCC
    CAGCAACCCG TGAGTGAAGT TGCTACCGTC CCGTGTCCTA GAATTGATAC TCAGCAACTG
    GACCGGCAAA TTAAAGCAAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT
    GAAGTATGTT CTTCTCAACT TCTAACCTCA GTAAGACGCA TGGTTTTGGT CCTTACTCAG
    CAAAACAATG AGAGCAAAGA GTTTGTAAAG TTCTTTCATA AGCAGCTTGG AAGTATATTA
    CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAGG ATTGTGGAGA AGATCTTCTT
    GTAGAGATAT CTGAAGTGTT ATTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG
    GATAATAATA GTGTAACTGT TAAACAGAGA TGCAAAAGGA AAATAGAAGC TGCTGGAGTG
    ATACAGTCTT ATGCAAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCACAG TTCACCTGCT
    GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAGACGG AAACAGTTAA GCAGACTCAA
    ACGTCTGAGG TTTATGATGG TGACGGCCCC AAAAATGTGA GATCTGATAT TTCTGATCAA
    GAGGATGATG AAGAAAGTGA AGAATGTCCA GTGTCTATTA GTTTGTCCAA AGCTGAAACT
    CAGGCTTTGA CTAATTACGG AAGTGGAGAA GATGAGAATG AAGATGAAGA GATAGAAGAA
    TTTGAAGAGG GACCTGTAGA TGTCCAGACG TCACTTCAGG CTAACACTGA AACTACAGAA
    GAAAATGAAC ACGATGATCA GGTTCCACAG CATGATTTTG AAAAATCATC AGAAAGCAAA
    AATGTCCCAT CAGAACAAGA ACCCACTACT AGTAAAGACT TTCTTTTGAT GACAGGTGAC
    CAGGATAGCA CTCCTGTGAA GCCATGTTAC CTCAATATCG TGGAAAATGA GCAACCTTTA
    AATAGCCCTG TCCAGAAGGA CACTCTCACT ACCATCGACT CGTCCAGTCA ACCTAACCCT
    TTGCCTTTAC CTTTAACTGA AATTGAAACC TTGGTGCCTA GAGTCAAAGA AGTGAAATCT
    GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA
    GTGAATGACT ATGAAGCAGA ATCTGGTAAC ATAAGTCAAA AATCTGATGA AGAAGACTTT
    GTGAAAGTTG AAGATTTACC ACTTAAACTG ACAATATATT CAGAGGCAGA TCTAAGGAAG
    AAAATGGCCG AAGAAGAACA GAAAAACCAC TTATCTGGTG AAATATGTGC AATGCAGACT
    GAAGAATTAG CTGGGAATTC TCAGACACTA AAAGAACCTG AAACAGTGGG AGCCCAAAGT
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818586.1
    CDS463..6828
    Translation

    Target ORF information:

    RefSeq Version XM_010820284.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820284.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG CAGATTGTCG ATACAATAGA
    GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGATG AGGAGGAGGA GGAGGAGGAA
    GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT
    GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG
    AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA
    GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC
    ACTAGAGGAA ATGCCAATAA AACACAAAAA GATGCTAGAG TAAATGAAAA GGCAAGAGAG
    AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA
    AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC
    CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT
    ACTTCATCCC CAACCGTTAA TGAGAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT
    CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA
    AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG
    AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT
    GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT
    TCTACCCAGT GTGCCCTAGA TGAAGAAGGG GATGAAGATG GTTACCTTTC TGAAGGAATT
    ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT
    CATATGTATC CTCCAAACAG TGCAAATCGT AATTCTTACA ATGTAAAGGA AACTAAAAAT
    AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA
    AGACAACAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT
    TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT
    TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA
    CAAACTCTTC TCACAAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG
    CACCTTATAA TGCACCAGTT GAACCAGTGC TATACCCAGC TAACGTGGCA GCAGAATAAT
    GTACAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCACC AACAAAATCA GCATCCAGAA
    AAACCTGCAA GCAAGGAAGG AGGCAGTGGT GCATCACACC CTCCTTCTCC CAGTTTATTT
    TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG ATTTACTAAT
    TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT
    TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA
    GGGAAAACAG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCA
    GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG
    TTTTATGACC AAGAAGGTGA GGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT
    GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC
    CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA
    ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA
    GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTG CTCAGCCGAA AAGCAGAGTG
    AAAAATATTG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA
    AACAGACATT CAGCCCAGAC CGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAA
    CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGCACAT
    GCTAGGAGAA TACTACAGCA GTCTAACAGA AATGCATGCA ATGAAGCGCC AGAAACCAGT
    AGTGATTTTT CCATGTTCGA AGCTTTGCGG GATACCATTT ATTCTGAAGT AGCTACGTTG
    ATTTCTCAAA ATGAATCTCG TCCACATTTT CTTATTGAAC TCTTCCATGA GCTTCAGCTA
    CTTAATACAG ACTATTTGAG ACAGAGGGCC TTATATGCAT TGCAGGACAT AGTATCCAGG
    CATATTTCTG AGAACCATGA AAAAGAAGGA GAAAATATAA AGTCCGTGAA TTCTGGTACT
    TGGATAGCAT CCAACTCAGA ACTTACTCCC AGTGAAAGCC TTGCTACTAC TGATGATGAA
    ACTTTTGAGA AGAACTTTGA GAGAGAAACA CATAAAATAA GTGAGCAAAA TGATGCTGAT
    AATGCTAGTG TATCTTCAAC TTGTGAGCCT TTTGCAACAG ATGTTCTGGG TGACACTGTG
    ATTCACTTAG ATCAGGCATT AGCCAGAATG AGAGACTACA CGCATATGAA GATGGAGGCT
    GAAAGTAGTC CAGACGTCAG GTGCACCTGC AGGATCGTTG AGGATGACGA TGGTGCTGCT
    GCCACGACCA CGATTAGTAA TAATTTAGAA GAAACTCCTG TTATTGAAAA TCATAGTTCC
    CAGCAACCCG TGAGTGAAGT TGCTACCGTC CCGTGTCCTA GAATTGATAC TCAGCAACTG
    GACCGGCAAA TTAAAGCAAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT
    GAAGTATGTT CTTCTCAACT TCTAACCTCA GTAAGACGCA TGGTTTTGGT CCTTACTCAG
    CAAAACAATG AGAGCAAAGA GTTTGTAAAG TTCTTTCATA AGCAGCTTGG AAGTATATTA
    CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAGG ATTGTGGAGA AGATCTTCTT
    GTAGAGATAT CTGAAGTGTT ATTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG
    GATAATAATA GTGTAACTGT TAAACAGAGA TGCAAAAGGA AAATAGAAGC TGCTGGAGTG
    ATACAGTCTT ATGCAAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCACAG TTCACCTGCT
    GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAGACGG AAACAGTTAA GCAGACTCAA
    ACGTCTGAGG TTTATGATGG TGACGGCCCC AAAAATGTGA GATCTGATAT TTCTGATCAA
    GAGGATGATG AAGAAAGTGA AGAATGTCCA GTGTCTATTA GTTTGTCCAA AGCTGAAACT
    CAGGCTTTGA CTAATTACGG AAGTGGAGAA GATGAGAATG AAGATGAAGA GATAGAAGAA
    TTTGAAGAGG GACCTGTAGA TGTCCAGACG TCACTTCAGG CTAACACTGA AACTACAGAA
    GAAAATGAAC ACGATGATCA GGTTCCACAG CATGATTTTG AAAAATCATC AGAAAGCAAA
    AATGTCCCAT CAGAACAAGA ACCCACTACT AGTAAAGACT TTCTTTTGAT GACAGGTGAC
    CAGGATAGCA CTCCTGTGAA GCCATGTTAC CTCAATATCG TGGAAAATGA GCAACCTTTA
    AATAGCCCTG TCCAGAAGGA CACTCTCACT ACCATCGACT CGTCCAGTCA ACCTAACCCT
    TTGCCTTTAC CTTTAACTGA AATTGAAACC TTGGTGCCTA GAGTCAAAGA AGTGAAATCT
    GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA
    GTGAATGACT ATGAAGCAGA ATCTGGTAAC ATAAGTCAAA AATCTGATGA AGAAGACTTT
    GTGAAAGTTG AAGATTTACC ACTTAAACTG ACAATATATT CAGAGGCAGA TCTAAGGAAG
    AAAATGGCCG AAGAAGAACA GAAAAACCAC TTATCTGGTG AAATATGTGC AATGCAGACT
    GAAGAATTAG CTGGGAATTC TCAGACACTA AAAGAACCTG AAACAGTGGG AGCCCAAAGT
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50990
    Clone ID Related Accession (Same CDS sequence) XM_010820284.2 , XM_010826088.2
    Accession Version XM_010826088.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646718.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826088.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG CAGATTGTCG ATACAATAGA
    GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGATG AGGAGGAGGA GGAGGAGGAA
    GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT
    GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG
    AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA
    GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC
    ACTAGAGGAA ATGCCAATAA AACACAAAAA GATGCTAGAG TAAATGAAAA GGCAAGAGAG
    AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA
    AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC
    CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT
    ACTTCATCCC CAACCGTTAA TGAGAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT
    CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA
    AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG
    AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT
    GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT
    TCTACCCAGT GTGCCCTAGA TGAAGAAGGG GATGAAGATG GTTACCTTTC TGAAGGAATT
    ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT
    CATATGTATC CTCCAAACAG TGCAAATCGT AATTCTTACA ATGTAAAGGA AACTAAAAAT
    AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA
    AGACAACAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT
    TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT
    TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA
    CAAACTCTTC TCACAAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG
    CACCTTATAA TGCACCAGTT GAACCAGTGC TATACCCAGC TAACGTGGCA GCAGAATAAT
    GTACAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCACC AACAAAATCA GCATCCAGAA
    AAACCTGCAA GCAAGGAAGG AGGCAGTGGT GCATCACACC CTCCTTCTCC CAGTTTATTT
    TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG ATTTACTAAT
    TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT
    TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA
    GGGAAAACAG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCA
    GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG
    TTTTATGACC AAGAAGGTGA GGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT
    GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC
    CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA
    ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA
    GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTG CTCAGCCGAA AAGCAGAGTG
    AAAAATATTG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA
    AACAGACATT CAGCCCAGAC CGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAA
    CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGCACAT
    GCTAGGAGAA TACTACAGCA GTCTAACAGA AATGCATGCA ATGAAGCGCC AGAAACCAGT
    AGTGATTTTT CCATGTTCGA AGCTTTGCGG GATACCATTT ATTCTGAAGT AGCTACGTTG
    ATTTCTCAAA ATGAATCTCG TCCACATTTT CTTATTGAAC TCTTCCATGA GCTTCAGCTA
    CTTAATACAG ACTATTTGAG ACAGAGGGCC TTATATGCAT TGCAGGACAT AGTATCCAGG
    CATATTTCTG AGAACCATGA AAAAGAAGGA GAAAATATAA AGTCCGTGAA TTCTGGTACT
    TGGATAGCAT CCAACTCAGA ACTTACTCCC AGTGAAAGCC TTGCTACTAC TGATGATGAA
    ACTTTTGAGA AGAACTTTGA GAGAGAAACA CATAAAATAA GTGAGCAAAA TGATGCTGAT
    AATGCTAGTG TATCTTCAAC TTGTGAGCCT TTTGCAACAG ATGTTCTGGG TGACACTGTG
    ATTCACTTAG ATCAGGCATT AGCCAGAATG AGAGACTACA CGCATATGAA GATGGAGGCT
    GAAAGTAGTC CAGACGTCAG GTGCACCTGC AGGATCGTTG AGGATGACGA TGGTGCTGCT
    GCCACGACCA CGATTAGTAA TAATTTAGAA GAAACTCCTG TTATTGAAAA TCATAGTTCC
    CAGCAACCCG TGAGTGAAGT TGCTACCGTC CCGTGTCCTA GAATTGATAC TCAGCAACTG
    GACCGGCAAA TTAAAGCAAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT
    GAAGTATGTT CTTCTCAACT TCTAACCTCA GTAAGACGCA TGGTTTTGGT CCTTACTCAG
    CAAAACAATG AGAGCAAAGA GTTTGTAAAG TTCTTTCATA AGCAGCTTGG AAGTATATTA
    CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAGG ATTGTGGAGA AGATCTTCTT
    GTAGAGATAT CTGAAGTGTT ATTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG
    GATAATAATA GTGTAACTGT TAAACAGAGA TGCAAAAGGA AAATAGAAGC TGCTGGAGTG
    ATACAGTCTT ATGCAAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCACAG TTCACCTGCT
    GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAGACGG AAACAGTTAA GCAGACTCAA
    ACGTCTGAGG TTTATGATGG TGACGGCCCC AAAAATGTGA GATCTGATAT TTCTGATCAA
    GAGGATGATG AAGAAAGTGA AGAATGTCCA GTGTCTATTA GTTTGTCCAA AGCTGAAACT
    CAGGCTTTGA CTAATTACGG AAGTGGAGAA GATGAGAATG AAGATGAAGA GATAGAAGAA
    TTTGAAGAGG GACCTGTAGA TGTCCAGACG TCACTTCAGG CTAACACTGA AACTACAGAA
    GAAAATGAAC ACGATGATCA GGTTCCACAG CATGATTTTG AAAAATCATC AGAAAGCAAA
    AATGTCCCAT CAGAACAAGA ACCCACTACT AGTAAAGACT TTCTTTTGAT GACAGGTGAC
    CAGGATAGCA CTCCTGTGAA GCCATGTTAC CTCAATATCG TGGAAAATGA GCAACCTTTA
    AATAGCCCTG TCCAGAAGGA CACTCTCACT ACCATCGACT CGTCCAGTCA ACCTAACCCT
    TTGCCTTTAC CTTTAACTGA AATTGAAACC TTGGTGCCTA GAGTCAAAGA AGTGAAATCT
    GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA
    GTGAATGACT ATGAAGCAGA ATCTGGTAAC ATAAGTCAAA AATCTGATGA AGAAGACTTT
    GTGAAAGTTG AAGATTTACC ACTTAAACTG ACAATATATT CAGAGGCAGA TCTAAGGAAG
    AAAATGGCCG AAGAAGAACA GAAAAACCAC TTATCTGGTG AAATATGTGC AATGCAGACT
    GAAGAATTAG CTGGGAATTC TCAGACACTA AAAGAACCTG AAACAGTGGG AGCCCAAAGT
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824390.1
    CDS463..6828
    Translation

    Target ORF information:

    RefSeq Version XM_010826088.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826088.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT
    TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCGGT CTGAGGCTTC AGATGCCACG
    GCCAGAGATC CTCAGCAGGA GCCTATAGAA GAGATAGAAA ATTTGAAGAA ACAACATGAT
    TTATTAAAAA GGATGTTACA GCAGCAGGAG CAGCTGAGAG CTCTACAGGG ACGACAAGCT
    GCTCTGCTTG CCCTGCAGCA TAAAGCCGAG CAGGCTATAG CTGTGATGGA TGATTCTGAA
    AAGGTTGCGG AGACAACTCC TGGCCATCAT ACTGTACCAG GCAGACAGCC GGCTCGTTCA
    CCTTTGCATC AGAGAGTACC CATAAGAGAA ACTACAGGTA GCCTATCTGG AGTCAGTATC
    ACCTCTGAAC TAAATGAAGA ATTGAATGAT TTAATTCAGC ATTTTCATAA TCAGCTTCGT
    GATTCTCAGC CTCCAGCTGT TCCAGACAAT AGAAGACAAG CAGAAAGTCT TTCATTAACT
    AGGGAGATTT CTCAGAGCAG AAATCCATCA GTTTCAGAGC ATTTACCTGA TGAGAAAGTC
    CAACTTTTTA GCAAGATGAG AGTGTTACAA GAAAAGAAAC AAAAAATGGA CAAACTTCTT
    GGAGAACTTC ATACACTTCG AGATCAGCAT TTGAACAATT CATCATTTGT GCCTTCTTCA
    GCCTCTCCAC AAAGGAGTGT GGATCAGAGA AGTACAACCA CAGCTCCTTC TGCTCCTGTA
    GTCTTAACAC CAGTGGTCAA TGGAGAATCC AATAACCTCA CGCCATCTGT TCCTTATCCT
    GCTGCTTCTC TAGTTTCTCA GAATCAGAAT GAAAATGAAG GCCACCTAAA TCCAACTGAA
    AAACTCCAGA AGTTAAATGA AGTTCGAAAG AGGTTGAATG AACTAAGAGA ATTAGTTCAT
    TATTATGAGC AAACATCAGA TATGATGACA GATGCTGTGA ATGAAAACAC AAAAGAAGAA
    GAAACTGAAG AGTCGGAATA TGATTCTGAG CATGAAAACT CTGAACCTGT TACTCATATT
    CGAAATCCAC AAGTGGCTGC CACCTGGAAC GAAGTAAATA GTAATTCTAA TGCACAGTGT
    GTTTCTAACA ATAGAGATGG GCGAGCAGTT AATTCTAACT GTGAAATTAA CAACAGGTCT
    GCTGCCAACA TAAGGGCTCT AAACATGCCT CCTCCTTCAG CAGATTGTCG ATACAATAGA
    GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGATG AGGAGGAGGA GGAGGAGGAA
    GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT
    GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG
    AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA
    GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC
    ACTAGAGGAA ATGCCAATAA AACACAAAAA GATGCTAGAG TAAATGAAAA GGCAAGAGAG
    AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA
    AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC
    CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT
    ACTTCATCCC CAACCGTTAA TGAGAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT
    CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA
    AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG
    AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT
    GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT
    TCTACCCAGT GTGCCCTAGA TGAAGAAGGG GATGAAGATG GTTACCTTTC TGAAGGAATT
    ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT
    CATATGTATC CTCCAAACAG TGCAAATCGT AATTCTTACA ATGTAAAGGA AACTAAAAAT
    AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA
    AGACAACAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT
    TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT
    TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA
    CAAACTCTTC TCACAAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG
    CACCTTATAA TGCACCAGTT GAACCAGTGC TATACCCAGC TAACGTGGCA GCAGAATAAT
    GTACAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCACC AACAAAATCA GCATCCAGAA
    AAACCTGCAA GCAAGGAAGG AGGCAGTGGT GCATCACACC CTCCTTCTCC CAGTTTATTT
    TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG ATTTACTAAT
    TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT
    TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA
    GGGAAAACAG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCA
    GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG
    TTTTATGACC AAGAAGGTGA GGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT
    GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC
    CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA
    ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA
    GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTG CTCAGCCGAA AAGCAGAGTG
    AAAAATATTG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA
    AACAGACATT CAGCCCAGAC CGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAA
    CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGCACAT
    GCTAGGAGAA TACTACAGCA GTCTAACAGA AATGCATGCA ATGAAGCGCC AGAAACCAGT
    AGTGATTTTT CCATGTTCGA AGCTTTGCGG GATACCATTT ATTCTGAAGT AGCTACGTTG
    ATTTCTCAAA ATGAATCTCG TCCACATTTT CTTATTGAAC TCTTCCATGA GCTTCAGCTA
    CTTAATACAG ACTATTTGAG ACAGAGGGCC TTATATGCAT TGCAGGACAT AGTATCCAGG
    CATATTTCTG AGAACCATGA AAAAGAAGGA GAAAATATAA AGTCCGTGAA TTCTGGTACT
    TGGATAGCAT CCAACTCAGA ACTTACTCCC AGTGAAAGCC TTGCTACTAC TGATGATGAA
    ACTTTTGAGA AGAACTTTGA GAGAGAAACA CATAAAATAA GTGAGCAAAA TGATGCTGAT
    AATGCTAGTG TATCTTCAAC TTGTGAGCCT TTTGCAACAG ATGTTCTGGG TGACACTGTG
    ATTCACTTAG ATCAGGCATT AGCCAGAATG AGAGACTACA CGCATATGAA GATGGAGGCT
    GAAAGTAGTC CAGACGTCAG GTGCACCTGC AGGATCGTTG AGGATGACGA TGGTGCTGCT
    GCCACGACCA CGATTAGTAA TAATTTAGAA GAAACTCCTG TTATTGAAAA TCATAGTTCC
    CAGCAACCCG TGAGTGAAGT TGCTACCGTC CCGTGTCCTA GAATTGATAC TCAGCAACTG
    GACCGGCAAA TTAAAGCAAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT
    GAAGTATGTT CTTCTCAACT TCTAACCTCA GTAAGACGCA TGGTTTTGGT CCTTACTCAG
    CAAAACAATG AGAGCAAAGA GTTTGTAAAG TTCTTTCATA AGCAGCTTGG AAGTATATTA
    CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAGG ATTGTGGAGA AGATCTTCTT
    GTAGAGATAT CTGAAGTGTT ATTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG
    GATAATAATA GTGTAACTGT TAAACAGAGA TGCAAAAGGA AAATAGAAGC TGCTGGAGTG
    ATACAGTCTT ATGCAAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCACAG TTCACCTGCT
    GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAGACGG AAACAGTTAA GCAGACTCAA
    ACGTCTGAGG TTTATGATGG TGACGGCCCC AAAAATGTGA GATCTGATAT TTCTGATCAA
    GAGGATGATG AAGAAAGTGA AGAATGTCCA GTGTCTATTA GTTTGTCCAA AGCTGAAACT
    CAGGCTTTGA CTAATTACGG AAGTGGAGAA GATGAGAATG AAGATGAAGA GATAGAAGAA
    TTTGAAGAGG GACCTGTAGA TGTCCAGACG TCACTTCAGG CTAACACTGA AACTACAGAA
    GAAAATGAAC ACGATGATCA GGTTCCACAG CATGATTTTG AAAAATCATC AGAAAGCAAA
    AATGTCCCAT CAGAACAAGA ACCCACTACT AGTAAAGACT TTCTTTTGAT GACAGGTGAC
    CAGGATAGCA CTCCTGTGAA GCCATGTTAC CTCAATATCG TGGAAAATGA GCAACCTTTA
    AATAGCCCTG TCCAGAAGGA CACTCTCACT ACCATCGACT CGTCCAGTCA ACCTAACCCT
    TTGCCTTTAC CTTTAACTGA AATTGAAACC TTGGTGCCTA GAGTCAAAGA AGTGAAATCT
    GCTCAGGAGA CTCCTGAAAG TTCTTTGGCT GGAAGTCCTG ATACTGAATC TCCAGTGTTA
    GTGAATGACT ATGAAGCAGA ATCTGGTAAC ATAAGTCAAA AATCTGATGA AGAAGACTTT
    GTGAAAGTTG AAGATTTACC ACTTAAACTG ACAATATATT CAGAGGCAGA TCTAAGGAAG
    AAAATGGCCG AAGAAGAACA GAAAAACCAC TTATCTGGTG AAATATGTGC AATGCAGACT
    GAAGAATTAG CTGGGAATTC TCAGACACTA AAAGAACCTG AAACAGTGGG AGCCCAAAGT
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50991
    Clone ID Related Accession (Same CDS sequence) XM_010826089.2 , XM_010820285.2
    Accession Version XM_010826089.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646718.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGCAGATTG TCGATACAAT
    AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG ATGAGGAGGA GGAGGAGGAG
    GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC
    ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA
    CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT
    CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT
    AACACTAGAG GAAATGCCAA TAAAACACAA AAAGATGCTA GAGTAAATGA AAAGGCAAGA
    GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA
    GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT
    AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT
    GTTACTTCAT CCCCAACCGT TAATGAGAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG
    CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG
    TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG
    AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA
    TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA
    GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGGGATGAAG ATGGTTACCT TTCTGAAGGA
    ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC
    TTTCATATGT ATCCTCCAAA CAGTGCAAAT CGTAATTCTT ACAATGTAAA GGAAACTAAA
    AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG
    ACAAGACAAC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC
    TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT
    GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG
    CTACAAACTC TTCTCACAAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA
    GTGCACCTTA TAATGCACCA GTTGAACCAG TGCTATACCC AGCTAACGTG GCAGCAGAAT
    AATGTACAGA GGTTGAAACA AATGCTCAGT GAACTCATGC ACCAACAAAA TCAGCATCCA
    GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTGCATCAC ACCCTCCTTC TCCCAGTTTA
    TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGATTTACT
    AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA
    GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT
    TCAGGGAAAA CAGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA
    GCAGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA
    TGGTTTTATG ACCAAGAAGG TGAGGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG
    TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA
    CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA
    ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT
    AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTGCTCAGCC GAAAAGCAGA
    GTGAAAAATA TTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT
    AGAAACAGAC ATTCAGCCCA GACCGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG
    AAACATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGCA
    CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACC
    AGTAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG
    TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG
    CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC
    AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT
    ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT
    GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT
    GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGTGACACT
    GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ACACGCATAT GAAGATGGAG
    GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCG TTGAGGATGA CGATGGTGCT
    GCTGCCACGA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT
    TCCCAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA
    CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG
    GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT
    CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA
    TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT
    CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT
    TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA
    GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT
    GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT
    CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TATTTCTGAT
    CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAGTTTGTC CAAAGCTGAA
    ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAGATAGAA
    GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA
    GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC
    AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG ACTTTCTTTT GATGACAGGT
    GACCAGGATA GCACTCCTGT GAAGCCATGT TACCTCAATA TCGTGGAAAA TGAGCAACCT
    TTAAATAGCC CTGTCCAGAA GGACACTCTC ACTACCATCG ACTCGTCCAG TCAACCTAAC
    CCTTTGCCTT TACCTTTAAC TGAAATTGAA ACCTTGGTGC CTAGAGTCAA AGAAGTGAAA
    TCTGCTCAGG AGACTCCTGA AAGTTCTTTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG
    TTAGTGAATG ACTATGAAGC AGAATCTGGT AACATAAGTC AAAAATCTGA TGAAGAAGAC
    TTTGTGAAAG TTGAAGATTT ACCACTTAAA CTGACAATAT ATTCAGAGGC AGATCTAAGG
    AAGAAAATGG CCGAAGAAGA ACAGAAAAAC CACTTATCTG GTGAAATATG TGCAATGCAG
    ACTGAAGAAT TAGCTGGGAA TTCTCAGACA CTAAAAGAAC CTGAAACAGT GGGAGCCCAA
    AGTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824391.1
    CDS463..6711
    Translation

    Target ORF information:

    RefSeq Version XM_010826089.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826089.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGCAGATTG TCGATACAAT
    AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG ATGAGGAGGA GGAGGAGGAG
    GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC
    ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA
    CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT
    CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT
    AACACTAGAG GAAATGCCAA TAAAACACAA AAAGATGCTA GAGTAAATGA AAAGGCAAGA
    GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA
    GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT
    AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT
    GTTACTTCAT CCCCAACCGT TAATGAGAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG
    CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG
    TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG
    AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA
    TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA
    GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGGGATGAAG ATGGTTACCT TTCTGAAGGA
    ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC
    TTTCATATGT ATCCTCCAAA CAGTGCAAAT CGTAATTCTT ACAATGTAAA GGAAACTAAA
    AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG
    ACAAGACAAC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC
    TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT
    GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG
    CTACAAACTC TTCTCACAAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA
    GTGCACCTTA TAATGCACCA GTTGAACCAG TGCTATACCC AGCTAACGTG GCAGCAGAAT
    AATGTACAGA GGTTGAAACA AATGCTCAGT GAACTCATGC ACCAACAAAA TCAGCATCCA
    GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTGCATCAC ACCCTCCTTC TCCCAGTTTA
    TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGATTTACT
    AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA
    GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT
    TCAGGGAAAA CAGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA
    GCAGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA
    TGGTTTTATG ACCAAGAAGG TGAGGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG
    TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA
    CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA
    ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT
    AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTGCTCAGCC GAAAAGCAGA
    GTGAAAAATA TTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT
    AGAAACAGAC ATTCAGCCCA GACCGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG
    AAACATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGCA
    CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACC
    AGTAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG
    TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG
    CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC
    AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT
    ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT
    GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT
    GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGTGACACT
    GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ACACGCATAT GAAGATGGAG
    GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCG TTGAGGATGA CGATGGTGCT
    GCTGCCACGA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT
    TCCCAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA
    CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG
    GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT
    CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA
    TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT
    CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT
    TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA
    GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT
    GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT
    CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TATTTCTGAT
    CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAGTTTGTC CAAAGCTGAA
    ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAGATAGAA
    GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA
    GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC
    AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG ACTTTCTTTT GATGACAGGT
    GACCAGGATA GCACTCCTGT GAAGCCATGT TACCTCAATA TCGTGGAAAA TGAGCAACCT
    TTAAATAGCC CTGTCCAGAA GGACACTCTC ACTACCATCG ACTCGTCCAG TCAACCTAAC
    CCTTTGCCTT TACCTTTAAC TGAAATTGAA ACCTTGGTGC CTAGAGTCAA AGAAGTGAAA
    TCTGCTCAGG AGACTCCTGA AAGTTCTTTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG
    TTAGTGAATG ACTATGAAGC AGAATCTGGT AACATAAGTC AAAAATCTGA TGAAGAAGAC
    TTTGTGAAAG TTGAAGATTT ACCACTTAAA CTGACAATAT ATTCAGAGGC AGATCTAAGG
    AAGAAAATGG CCGAAGAAGA ACAGAAAAAC CACTTATCTG GTGAAATATG TGCAATGCAG
    ACTGAAGAAT TAGCTGGGAA TTCTCAGACA CTAAAAGAAC CTGAAACAGT GGGAGCCCAA
    AGTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50991
    Clone ID Related Accession (Same CDS sequence) XM_010826089.2 , XM_010820285.2
    Accession Version XM_010820285.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGCAGATTG TCGATACAAT
    AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG ATGAGGAGGA GGAGGAGGAG
    GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC
    ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA
    CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT
    CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT
    AACACTAGAG GAAATGCCAA TAAAACACAA AAAGATGCTA GAGTAAATGA AAAGGCAAGA
    GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA
    GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT
    AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT
    GTTACTTCAT CCCCAACCGT TAATGAGAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG
    CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG
    TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG
    AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA
    TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA
    GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGGGATGAAG ATGGTTACCT TTCTGAAGGA
    ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC
    TTTCATATGT ATCCTCCAAA CAGTGCAAAT CGTAATTCTT ACAATGTAAA GGAAACTAAA
    AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG
    ACAAGACAAC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC
    TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT
    GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG
    CTACAAACTC TTCTCACAAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA
    GTGCACCTTA TAATGCACCA GTTGAACCAG TGCTATACCC AGCTAACGTG GCAGCAGAAT
    AATGTACAGA GGTTGAAACA AATGCTCAGT GAACTCATGC ACCAACAAAA TCAGCATCCA
    GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTGCATCAC ACCCTCCTTC TCCCAGTTTA
    TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGATTTACT
    AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA
    GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT
    TCAGGGAAAA CAGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA
    GCAGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA
    TGGTTTTATG ACCAAGAAGG TGAGGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG
    TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA
    CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA
    ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT
    AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTGCTCAGCC GAAAAGCAGA
    GTGAAAAATA TTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT
    AGAAACAGAC ATTCAGCCCA GACCGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG
    AAACATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGCA
    CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACC
    AGTAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG
    TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG
    CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC
    AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT
    ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT
    GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT
    GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGTGACACT
    GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ACACGCATAT GAAGATGGAG
    GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCG TTGAGGATGA CGATGGTGCT
    GCTGCCACGA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT
    TCCCAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA
    CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG
    GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT
    CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA
    TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT
    CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT
    TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA
    GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT
    GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT
    CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TATTTCTGAT
    CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAGTTTGTC CAAAGCTGAA
    ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAGATAGAA
    GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA
    GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC
    AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG ACTTTCTTTT GATGACAGGT
    GACCAGGATA GCACTCCTGT GAAGCCATGT TACCTCAATA TCGTGGAAAA TGAGCAACCT
    TTAAATAGCC CTGTCCAGAA GGACACTCTC ACTACCATCG ACTCGTCCAG TCAACCTAAC
    CCTTTGCCTT TACCTTTAAC TGAAATTGAA ACCTTGGTGC CTAGAGTCAA AGAAGTGAAA
    TCTGCTCAGG AGACTCCTGA AAGTTCTTTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG
    TTAGTGAATG ACTATGAAGC AGAATCTGGT AACATAAGTC AAAAATCTGA TGAAGAAGAC
    TTTGTGAAAG TTGAAGATTT ACCACTTAAA CTGACAATAT ATTCAGAGGC AGATCTAAGG
    AAGAAAATGG CCGAAGAAGA ACAGAAAAAC CACTTATCTG GTGAAATATG TGCAATGCAG
    ACTGAAGAAT TAGCTGGGAA TTCTCAGACA CTAAAAGAAC CTGAAACAGT GGGAGCCCAA
    AGTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818587.1
    CDS463..6711
    Translation

    Target ORF information:

    RefSeq Version XM_010820285.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820285.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGCAGATTG TCGATACAAT
    AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG ATGAGGAGGA GGAGGAGGAG
    GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC
    ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA
    CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT
    CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT
    AACACTAGAG GAAATGCCAA TAAAACACAA AAAGATGCTA GAGTAAATGA AAAGGCAAGA
    GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA
    GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT
    AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT
    GTTACTTCAT CCCCAACCGT TAATGAGAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG
    CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG
    TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG
    AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA
    TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA
    GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGGGATGAAG ATGGTTACCT TTCTGAAGGA
    ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC
    TTTCATATGT ATCCTCCAAA CAGTGCAAAT CGTAATTCTT ACAATGTAAA GGAAACTAAA
    AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG
    ACAAGACAAC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC
    TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT
    GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG
    CTACAAACTC TTCTCACAAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA
    GTGCACCTTA TAATGCACCA GTTGAACCAG TGCTATACCC AGCTAACGTG GCAGCAGAAT
    AATGTACAGA GGTTGAAACA AATGCTCAGT GAACTCATGC ACCAACAAAA TCAGCATCCA
    GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTGCATCAC ACCCTCCTTC TCCCAGTTTA
    TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGATTTACT
    AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA
    GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT
    TCAGGGAAAA CAGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA
    GCAGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA
    TGGTTTTATG ACCAAGAAGG TGAGGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG
    TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA
    CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA
    ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT
    AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTGCTCAGCC GAAAAGCAGA
    GTGAAAAATA TTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT
    AGAAACAGAC ATTCAGCCCA GACCGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG
    AAACATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGCA
    CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC GCCAGAAACC
    AGTAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG
    TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG
    CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC
    AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT
    ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT
    GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT
    GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGTGACACT
    GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ACACGCATAT GAAGATGGAG
    GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCG TTGAGGATGA CGATGGTGCT
    GCTGCCACGA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT
    TCCCAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA
    CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG
    GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT
    CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA
    TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT
    CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT
    TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA
    GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT
    GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT
    CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TATTTCTGAT
    CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAGTTTGTC CAAAGCTGAA
    ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAGATAGAA
    GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA
    GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC
    AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG ACTTTCTTTT GATGACAGGT
    GACCAGGATA GCACTCCTGT GAAGCCATGT TACCTCAATA TCGTGGAAAA TGAGCAACCT
    TTAAATAGCC CTGTCCAGAA GGACACTCTC ACTACCATCG ACTCGTCCAG TCAACCTAAC
    CCTTTGCCTT TACCTTTAAC TGAAATTGAA ACCTTGGTGC CTAGAGTCAA AGAAGTGAAA
    TCTGCTCAGG AGACTCCTGA AAGTTCTTTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG
    TTAGTGAATG ACTATGAAGC AGAATCTGGT AACATAAGTC AAAAATCTGA TGAAGAAGAC
    TTTGTGAAAG TTGAAGATTT ACCACTTAAA CTGACAATAT ATTCAGAGGC AGATCTAAGG
    AAGAAAATGG CCGAAGAAGA ACAGAAAAAC CACTTATCTG GTGAAATATG TGCAATGCAG
    ACTGAAGAAT TAGCTGGGAA TTCTCAGACA CTAAAAGAAC CTGAAACAGT GGGAGCCCAA
    AGTACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50992
    Clone ID Related Accession (Same CDS sequence) XM_010820286.2 , XM_010826090.2 , XM_010820286.3
    Accession Version XM_010820286.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820286.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGCAGATTG TCGATACAAT
    AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG ATGAGGAGGA GGAGGAGGAG
    GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC
    ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA
    CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT
    CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT
    AACACTAGAG GAAATGCCAA TAAAACACAA AAAGATGCTA GAGTAAATGA AAAGGCAAGA
    GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA
    GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT
    AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT
    GTTACTTCAT CCCCAACCGT TAATGAGAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG
    CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG
    TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG
    AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA
    TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA
    GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGGGATGAAG ATGGTTACCT TTCTGAAGGA
    ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC
    TTTCATATGT ATCCTCCAAA CAGTGCAAAT CGTAATTCTT ACAATGTAAA GGAAACTAAA
    AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG
    ACAAGACAAC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC
    TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT
    GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG
    CTACAAACTC TTCTCACAAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA
    GTGCACCTTA TAATGCACCA GTTGAACCAG TGCTATACCC AGCTAACGTG GCAGCAGAAT
    AATGTACAGA GGTTGAAACA AATGCTCAGT GAACTCATGC ACCAACAAAA TCAGCATCCA
    GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTGCATCAC ACCCTCCTTC TCCCAGTTTA
    TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGATTTACT
    AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA
    GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT
    TCAGGGAAAA CAGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA
    GCAGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA
    TGGTTTTATG ACCAAGAAGG TGAGGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG
    TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA
    CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA
    ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT
    AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTGCTCAGCC GAAAAGCAGA
    GTGAAAAATA TTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT
    AGAAACAGAC ATTCAGCCCA GACCGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG
    AAACATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGAA
    ACCAGTAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT
    ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT
    CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA
    TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT
    GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT
    GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT
    GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGTGAC
    ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTACACGCA TATGAAGATG
    GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCGTTGAGGA TGACGATGGT
    GCTGCTGCCA CGACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT
    AGTTCCCAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG
    CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC
    ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT
    ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT
    ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT
    CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA
    GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT
    GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA
    CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGGTGACCA GGATAGCACT
    CCTGTGAAGC CATGTTACCT CAATATCGTG GAAAATGAGC AACCTTTAAA TAGCCCTGTC
    CAGAAGGACA CTCTCACTAC CATCGACTCG TCCAGTCAAC CTAACCCTTT GCCTTTACCT
    TTAACTGAAA TTGAAACCTT GGTGCCTAGA GTCAAAGAAG TGAAATCTGC TCAGGAGACT
    CCTGAAAGTT CTTTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT
    GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA
    GATTTACCAC TTAAACTGAC AATATATTCA GAGGCAGATC TAAGGAAGAA AATGGCCGAA
    GAAGAACAGA AAAACCACTT ATCTGGTGAA ATATGTGCAA TGCAGACTGA AGAATTAGCT
    GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGTAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818588.1
    CDS462..6635
    Translation

    Target ORF information:

    RefSeq Version XM_010820286.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820286.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGCAGATTG TCGATACAAT
    AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG ATGAGGAGGA GGAGGAGGAG
    GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC
    ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA
    CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT
    CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT
    AACACTAGAG GAAATGCCAA TAAAACACAA AAAGATGCTA GAGTAAATGA AAAGGCAAGA
    GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA
    GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT
    AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT
    GTTACTTCAT CCCCAACCGT TAATGAGAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG
    CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG
    TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG
    AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA
    TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA
    GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGGGATGAAG ATGGTTACCT TTCTGAAGGA
    ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC
    TTTCATATGT ATCCTCCAAA CAGTGCAAAT CGTAATTCTT ACAATGTAAA GGAAACTAAA
    AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG
    ACAAGACAAC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC
    TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT
    GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG
    CTACAAACTC TTCTCACAAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA
    GTGCACCTTA TAATGCACCA GTTGAACCAG TGCTATACCC AGCTAACGTG GCAGCAGAAT
    AATGTACAGA GGTTGAAACA AATGCTCAGT GAACTCATGC ACCAACAAAA TCAGCATCCA
    GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTGCATCAC ACCCTCCTTC TCCCAGTTTA
    TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGATTTACT
    AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA
    GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT
    TCAGGGAAAA CAGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA
    GCAGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA
    TGGTTTTATG ACCAAGAAGG TGAGGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG
    TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA
    CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA
    ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT
    AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTGCTCAGCC GAAAAGCAGA
    GTGAAAAATA TTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT
    AGAAACAGAC ATTCAGCCCA GACCGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG
    AAACATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGAA
    ACCAGTAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT
    ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT
    CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA
    TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT
    GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT
    GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT
    GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGTGAC
    ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTACACGCA TATGAAGATG
    GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCGTTGAGGA TGACGATGGT
    GCTGCTGCCA CGACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT
    AGTTCCCAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG
    CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC
    ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT
    ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT
    ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT
    CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA
    GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT
    GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA
    CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGGTGACCA GGATAGCACT
    CCTGTGAAGC CATGTTACCT CAATATCGTG GAAAATGAGC AACCTTTAAA TAGCCCTGTC
    CAGAAGGACA CTCTCACTAC CATCGACTCG TCCAGTCAAC CTAACCCTTT GCCTTTACCT
    TTAACTGAAA TTGAAACCTT GGTGCCTAGA GTCAAAGAAG TGAAATCTGC TCAGGAGACT
    CCTGAAAGTT CTTTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT
    GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA
    GATTTACCAC TTAAACTGAC AATATATTCA GAGGCAGATC TAAGGAAGAA AATGGCCGAA
    GAAGAACAGA AAAACCACTT ATCTGGTGAA ATATGTGCAA TGCAGACTGA AGAATTAGCT
    GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGTAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50992
    Clone ID Related Accession (Same CDS sequence) XM_010820286.2 , XM_010826090.2 , XM_010820286.3
    Accession Version XM_010826090.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646718.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826090.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGCAGATTG TCGATACAAT
    AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG ATGAGGAGGA GGAGGAGGAG
    GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC
    ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA
    CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT
    CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT
    AACACTAGAG GAAATGCCAA TAAAACACAA AAAGATGCTA GAGTAAATGA AAAGGCAAGA
    GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA
    GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT
    AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT
    GTTACTTCAT CCCCAACCGT TAATGAGAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG
    CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG
    TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG
    AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA
    TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA
    GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGGGATGAAG ATGGTTACCT TTCTGAAGGA
    ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC
    TTTCATATGT ATCCTCCAAA CAGTGCAAAT CGTAATTCTT ACAATGTAAA GGAAACTAAA
    AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG
    ACAAGACAAC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC
    TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT
    GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG
    CTACAAACTC TTCTCACAAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA
    GTGCACCTTA TAATGCACCA GTTGAACCAG TGCTATACCC AGCTAACGTG GCAGCAGAAT
    AATGTACAGA GGTTGAAACA AATGCTCAGT GAACTCATGC ACCAACAAAA TCAGCATCCA
    GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTGCATCAC ACCCTCCTTC TCCCAGTTTA
    TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGATTTACT
    AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA
    GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT
    TCAGGGAAAA CAGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA
    GCAGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA
    TGGTTTTATG ACCAAGAAGG TGAGGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG
    TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA
    CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA
    ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT
    AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTGCTCAGCC GAAAAGCAGA
    GTGAAAAATA TTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT
    AGAAACAGAC ATTCAGCCCA GACCGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG
    AAACATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGAA
    ACCAGTAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT
    ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT
    CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA
    TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT
    GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT
    GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT
    GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGTGAC
    ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTACACGCA TATGAAGATG
    GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCGTTGAGGA TGACGATGGT
    GCTGCTGCCA CGACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT
    AGTTCCCAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG
    CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC
    ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT
    ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT
    ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT
    CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA
    GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT
    GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA
    CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGGTGACCA GGATAGCACT
    CCTGTGAAGC CATGTTACCT CAATATCGTG GAAAATGAGC AACCTTTAAA TAGCCCTGTC
    CAGAAGGACA CTCTCACTAC CATCGACTCG TCCAGTCAAC CTAACCCTTT GCCTTTACCT
    TTAACTGAAA TTGAAACCTT GGTGCCTAGA GTCAAAGAAG TGAAATCTGC TCAGGAGACT
    CCTGAAAGTT CTTTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT
    GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA
    GATTTACCAC TTAAACTGAC AATATATTCA GAGGCAGATC TAAGGAAGAA AATGGCCGAA
    GAAGAACAGA AAAACCACTT ATCTGGTGAA ATATGTGCAA TGCAGACTGA AGAATTAGCT
    GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGTAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824392.1
    CDS462..6635
    Translation

    Target ORF information:

    RefSeq Version XM_010826090.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826090.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGCAGATTG TCGATACAAT
    AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG ATGAGGAGGA GGAGGAGGAG
    GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC
    ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA
    CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT
    CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT
    AACACTAGAG GAAATGCCAA TAAAACACAA AAAGATGCTA GAGTAAATGA AAAGGCAAGA
    GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA
    GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT
    AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT
    GTTACTTCAT CCCCAACCGT TAATGAGAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG
    CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG
    TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG
    AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA
    TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA
    GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGGGATGAAG ATGGTTACCT TTCTGAAGGA
    ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC
    TTTCATATGT ATCCTCCAAA CAGTGCAAAT CGTAATTCTT ACAATGTAAA GGAAACTAAA
    AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG
    ACAAGACAAC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC
    TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT
    GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG
    CTACAAACTC TTCTCACAAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA
    GTGCACCTTA TAATGCACCA GTTGAACCAG TGCTATACCC AGCTAACGTG GCAGCAGAAT
    AATGTACAGA GGTTGAAACA AATGCTCAGT GAACTCATGC ACCAACAAAA TCAGCATCCA
    GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTGCATCAC ACCCTCCTTC TCCCAGTTTA
    TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGATTTACT
    AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA
    GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT
    TCAGGGAAAA CAGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA
    GCAGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA
    TGGTTTTATG ACCAAGAAGG TGAGGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG
    TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA
    CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA
    ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT
    AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTGCTCAGCC GAAAAGCAGA
    GTGAAAAATA TTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT
    AGAAACAGAC ATTCAGCCCA GACCGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG
    AAACATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGAA
    ACCAGTAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT
    ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT
    CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA
    TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT
    GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT
    GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT
    GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGTGAC
    ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTACACGCA TATGAAGATG
    GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCGTTGAGGA TGACGATGGT
    GCTGCTGCCA CGACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT
    AGTTCCCAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG
    CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC
    ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT
    ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT
    ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT
    CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA
    GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT
    GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA
    CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGGTGACCA GGATAGCACT
    CCTGTGAAGC CATGTTACCT CAATATCGTG GAAAATGAGC AACCTTTAAA TAGCCCTGTC
    CAGAAGGACA CTCTCACTAC CATCGACTCG TCCAGTCAAC CTAACCCTTT GCCTTTACCT
    TTAACTGAAA TTGAAACCTT GGTGCCTAGA GTCAAAGAAG TGAAATCTGC TCAGGAGACT
    CCTGAAAGTT CTTTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT
    GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA
    GATTTACCAC TTAAACTGAC AATATATTCA GAGGCAGATC TAAGGAAGAA AATGGCCGAA
    GAAGAACAGA AAAACCACTT ATCTGGTGAA ATATGTGCAA TGCAGACTGA AGAATTAGCT
    GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGTAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50992
    Clone ID Related Accession (Same CDS sequence) XM_010820286.2 , XM_010826090.2 , XM_010820286.3
    Accession Version XM_010820286.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820286.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGCAGATTG TCGATACAAT
    AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG ATGAGGAGGA GGAGGAGGAG
    GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC
    ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA
    CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT
    CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT
    AACACTAGAG GAAATGCCAA TAAAACACAA AAAGATGCTA GAGTAAATGA AAAGGCAAGA
    GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA
    GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT
    AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT
    GTTACTTCAT CCCCAACCGT TAATGAGAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG
    CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG
    TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG
    AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA
    TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA
    GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGGGATGAAG ATGGTTACCT TTCTGAAGGA
    ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC
    TTTCATATGT ATCCTCCAAA CAGTGCAAAT CGTAATTCTT ACAATGTAAA GGAAACTAAA
    AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG
    ACAAGACAAC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC
    TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT
    GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG
    CTACAAACTC TTCTCACAAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA
    GTGCACCTTA TAATGCACCA GTTGAACCAG TGCTATACCC AGCTAACGTG GCAGCAGAAT
    AATGTACAGA GGTTGAAACA AATGCTCAGT GAACTCATGC ACCAACAAAA TCAGCATCCA
    GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTGCATCAC ACCCTCCTTC TCCCAGTTTA
    TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGATTTACT
    AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA
    GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT
    TCAGGGAAAA CAGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA
    GCAGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA
    TGGTTTTATG ACCAAGAAGG TGAGGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG
    TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA
    CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA
    ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT
    AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTGCTCAGCC GAAAAGCAGA
    GTGAAAAATA TTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT
    AGAAACAGAC ATTCAGCCCA GACCGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG
    AAACATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGAA
    ACCAGTAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT
    ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT
    CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA
    TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT
    GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT
    GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT
    GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGTGAC
    ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTACACGCA TATGAAGATG
    GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCGTTGAGGA TGACGATGGT
    GCTGCTGCCA CGACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT
    AGTTCCCAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG
    CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC
    ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT
    ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT
    ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT
    CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA
    GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT
    GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA
    CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGGTGACCA GGATAGCACT
    CCTGTGAAGC CATGTTACCT CAATATCGTG GAAAATGAGC AACCTTTAAA TAGCCCTGTC
    CAGAAGGACA CTCTCACTAC CATCGACTCG TCCAGTCAAC CTAACCCTTT GCCTTTACCT
    TTAACTGAAA TTGAAACCTT GGTGCCTAGA GTCAAAGAAG TGAAATCTGC TCAGGAGACT
    CCTGAAAGTT CTTTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT
    GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA
    GATTTACCAC TTAAACTGAC AATATATTCA GAGGCAGATC TAAGGAAGAA AATGGCCGAA
    GAAGAACAGA AAAACCACTT ATCTGGTGAA ATATGTGCAA TGCAGACTGA AGAATTAGCT
    GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGTAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818588.1
    CDS359..6532
    Translation

    Target ORF information:

    RefSeq Version XM_010820286.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820286.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGCAGATTG TCGATACAAT
    AGAGAAGGAG AACAGGAGAT GCCTGTTGCA CAAGGTCATG ATGAGGAGGA GGAGGAGGAG
    GAAGCAGAAG ATGAGGCCCT CAGTGGAGCT TCACTGTCTA GTCACAGCAG CAGCCTGATC
    ATTGAGACTC CTGAAGATGC TGAATTTGAA CATAAGATCC AGAAGCTTAT GGCTGCAAAA
    CTGAAACTTA GACACTTACA GAACCTTGTA GCCATGGTGC AGGACGATGA TGCAGCCAAT
    CAAGGAGTTA CTTCTGTGGA GGATTTCTTC CCAGCAGAAG ACACCAAACA AAATGCAAAT
    AACACTAGAG GAAATGCCAA TAAAACACAA AAAGATGCTA GAGTAAATGA AAAGGCAAGA
    GAGAAATTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AACTCAAACA ATTGCAGGAA
    GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG AATTGCAAAA GGCATGTCCT
    AACCTACAGC TGTCAGCTGC TAGTGTGGGT AATTGTCCCA CAAAAACACA TATGCCAGCT
    GTTACTTCAT CCCCAACCGT TAATGAGAAT GAAGCCAGTA CAAACAAATC TGGTTTTGAG
    CCTCATGATT CTTCAGTGGT AGATAATGAG GTATGGTCAG ATATGCGAAG ACATGAAATG
    TTAAGGGAGG AGCTACGACA GAGAAGAAAG CAGCTGGAAG CTCTGATGGC TGAACATCAG
    AGGAGGCAAG GTCTGGCTGA AACTGCATCT CCAGTGGCTG TTTCACTGAG AAGTGATGGA
    TCTGAGAACC TGTGTACACC TCAGCAAAGC AGAACAGAAA AAACAATGGC AACTTGGGGA
    GGTTCTACCC AGTGTGCCCT AGATGAAGAA GGGGATGAAG ATGGTTACCT TTCTGAAGGA
    ATTATTCGGA CAGACGAAGA GGAGGAAGAG GAGGAGCAAG ATGCCAGTTC CAATGATAAC
    TTTCATATGT ATCCTCCAAA CAGTGCAAAT CGTAATTCTT ACAATGTAAA GGAAACTAAA
    AATAGGTGGA AGAACAATCG CCCTTTTTCA GCAGATGGAA ATTATCGTCC TTTAGCCAAG
    ACAAGACAAC AGAATATCAG CATGCAGCGT CAGGAAAACC TTCGCTGGGT GTCCGAACTC
    TCTTATGTAG AAGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTAAA GAAACAACTT
    GATTTCAGTG TCAATATTTG TCAGACTTTA ATGCAAGACC AGCAGACTCT GTCTTGTCTG
    CTACAAACTC TTCTCACAAG TCCTTATAGT GTTATGCCCA GCAGTGTTGC ATCCCCGCAA
    GTGCACCTTA TAATGCACCA GTTGAACCAG TGCTATACCC AGCTAACGTG GCAGCAGAAT
    AATGTACAGA GGTTGAAACA AATGCTCAGT GAACTCATGC ACCAACAAAA TCAGCATCCA
    GAAAAACCTG CAAGCAAGGA AGGAGGCAGT GGTGCATCAC ACCCTCCTTC TCCCAGTTTA
    TTTTGTCCTT TCAACTTTCC AACTCAGCCT GTAAATCTGT TTAACCTACC GGGATTTACT
    AATTTTTCGT CATTTGCACC AAGTATGAAT TTCAGCCCTT TATTTCCTTC TAATTTTGGA
    GATTTTTCTC AAAATATTTC TACACCCACT GAACAGCAGC AACCGTTAGC CCAGAATCCT
    TCAGGGAAAA CAGAATACAT GGCTTTTCCA AAACCTTTTG AAAGTAGTTC TTCTATTGGA
    GCAGAAAAAC AAAGGAATCA AAAACAGCCT GAAGAGGAGG TGGAGAACAG TAGGACACCA
    TGGTTTTATG ACCAAGAAGG TGAGGTAGAA AAGCCATTTC TCAAGACTGG ATTTGCAGTG
    TCTGTAGAGA AAACAACAAA TAGTAATCGC AAAAATCAAC TAGATACCAG TAGGAGAAGA
    CGCCAGTTTG ATGAAGTGTC CTTAGAAAGC TTTAACAGTA TGCTTGACCC AGTAGATCCA
    ACAACAGTAA CTAAAACATT CAAGTCAAGA AAAGCATCTG CACAGGCCAG CCTGGCATCT
    AAAGATAAAA CTCCCAAATC AAAGAGTAAG AAGAGGCATT CTGCTCAGCC GAAAAGCAGA
    GTGAAAAATA TTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT
    AGAAACAGAC ATTCAGCCCA GACCGAAGAG CCTGTTCAAG CAAAAGTACT CAGCAGAAAG
    AAACATGAGC AGCTGGAAAA AGTCATAAGA TGTAGTAGGT CTACAGAAAT ATCTTCAGAA
    ACCAGTAGTG ATTTTTCCAT GTTCGAAGCT TTGCGGGATA CCATTTATTC TGAAGTAGCT
    ACGTTGATTT CTCAAAATGA ATCTCGTCCA CATTTTCTTA TTGAACTCTT CCATGAGCTT
    CAGCTACTTA ATACAGACTA TTTGAGACAG AGGGCCTTAT ATGCATTGCA GGACATAGTA
    TCCAGGCATA TTTCTGAGAA CCATGAAAAA GAAGGAGAAA ATATAAAGTC CGTGAATTCT
    GGTACTTGGA TAGCATCCAA CTCAGAACTT ACTCCCAGTG AAAGCCTTGC TACTACTGAT
    GATGAAACTT TTGAGAAGAA CTTTGAGAGA GAAACACATA AAATAAGTGA GCAAAATGAT
    GCTGATAATG CTAGTGTATC TTCAACTTGT GAGCCTTTTG CAACAGATGT TCTGGGTGAC
    ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGAG ACTACACGCA TATGAAGATG
    GAGGCTGAAA GTAGTCCAGA CGTCAGGTGC ACCTGCAGGA TCGTTGAGGA TGACGATGGT
    GCTGCTGCCA CGACCACGAT TAGTAATAAT TTAGAAGAAA CTCCTGTTAT TGAAAATCAT
    AGTTCCCAGC AACCCGTGAG TGAAGTTGCT ACCGTCCCGT GTCCTAGAAT TGATACTCAG
    CAACTGGACC GGCAAATTAA AGCAATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC
    ATGGATGAAG TATGTTCTTC TCAACTTCTA ACCTCAGTAA GACGCATGGT TTTGGTCCTT
    ACTCAGCAAA ACAATGAGAG CAAAGAGTTT GTAAAGTTCT TTCATAAGCA GCTTGGAAGT
    ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAGGATTG TGGAGAAGAT
    CTTCTTGTAG AGATATCTGA AGTGTTATTT AATGAATTGG CTTTCTTTAA GCTCATGCAA
    GATTTGGATA ATAATAGTGT AACTGTTAAA CAGAGATGCA AAAGGAAAAT AGAAGCTGCT
    GGAGTGATAC AGTCTTATGC AAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCACAGTTCA
    CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AGACGGAAAC AGTTAAGCAG
    ACTCAAACGT CTGAGGTTTA TGATGGTGAC GGCCCCAAAA ATGTGAGATC TGATATTTCT
    GATCAAGAGG ATGATGAAGA AAGTGAAGAA TGTCCAGTGT CTATTAGTTT GTCCAAAGCT
    GAAACTCAGG CTTTGACTAA TTACGGAAGT GGAGAAGATG AGAATGAAGA TGAAGAGATA
    GAAGAATTTG AAGAGGGACC TGTAGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAACT
    ACAGAAGAAA ATGAACACGA TGATCAGGTT CCACAGCATG ATTTTGAAAA ATCATCAGAA
    AGCAAAAATG TCCCATCAGA ACAAGAACCC ACTACTAGTA AAGGTGACCA GGATAGCACT
    CCTGTGAAGC CATGTTACCT CAATATCGTG GAAAATGAGC AACCTTTAAA TAGCCCTGTC
    CAGAAGGACA CTCTCACTAC CATCGACTCG TCCAGTCAAC CTAACCCTTT GCCTTTACCT
    TTAACTGAAA TTGAAACCTT GGTGCCTAGA GTCAAAGAAG TGAAATCTGC TCAGGAGACT
    CCTGAAAGTT CTTTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT
    GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA
    GATTTACCAC TTAAACTGAC AATATATTCA GAGGCAGATC TAAGGAAGAA AATGGCCGAA
    GAAGAACAGA AAAACCACTT ATCTGGTGAA ATATGTGCAA TGCAGACTGA AGAATTAGCT
    GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGTAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50993
    Clone ID Related Accession (Same CDS sequence) XM_010820287.2 , XM_010826091.2 , XM_010820287.3
    Accession Version XM_010820287.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGATTGTCG ATACAATAGA
    GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGATG AGGAGGAGGA GGAGGAGGAA
    GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT
    GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG
    AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA
    GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC
    ACTAGAGGAA ATGCCAATAA AACACAAAAA GATGCTAGAG TAAATGAAAA GGCAAGAGAG
    AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA
    AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC
    CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT
    ACTTCATCCC CAACCGTTAA TGAGAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT
    CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA
    AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG
    AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT
    GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT
    TCTACCCAGT GTGCCCTAGA TGAAGAAGGG GATGAAGATG GTTACCTTTC TGAAGGAATT
    ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT
    CATATGTATC CTCCAAACAG TGCAAATCGT AATTCTTACA ATGTAAAGGA AACTAAAAAT
    AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA
    AGACAACAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT
    TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT
    TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA
    CAAACTCTTC TCACAAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG
    CACCTTATAA TGCACCAGTT GAACCAGTGC TATACCCAGC TAACGTGGCA GCAGAATAAT
    GTACAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCACC AACAAAATCA GCATCCAGAA
    AAACCTGCAA GCAAGGAAGG AGGCAGTGGT GCATCACACC CTCCTTCTCC CAGTTTATTT
    TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG ATTTACTAAT
    TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT
    TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA
    GGGAAAACAG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCA
    GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG
    TTTTATGACC AAGAAGGTGA GGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT
    GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC
    CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA
    ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA
    GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTG CTCAGCCGAA AAGCAGAGTG
    AAAAATATTG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA
    AACAGACATT CAGCCCAGAC CGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAA
    CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGAAACC
    AGTAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG
    TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG
    CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC
    AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT
    ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT
    GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT
    GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGTGACACT
    GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ACACGCATAT GAAGATGGAG
    GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCG TTGAGGATGA CGATGGTGCT
    GCTGCCACGA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT
    TCCCAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA
    CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG
    GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT
    CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA
    TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT
    CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT
    TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA
    GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT
    GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT
    CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TATTTCTGAT
    CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAGTTTGTC CAAAGCTGAA
    ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAGATAGAA
    GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA
    GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC
    AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG GTGACCAGGA TAGCACTCCT
    GTGAAGCCAT GTTACCTCAA TATCGTGGAA AATGAGCAAC CTTTAAATAG CCCTGTCCAG
    AAGGACACTC TCACTACCAT CGACTCGTCC AGTCAACCTA ACCCTTTGCC TTTACCTTTA
    ACTGAAATTG AAACCTTGGT GCCTAGAGTC AAAGAAGTGA AATCTGCTCA GGAGACTCCT
    GAAAGTTCTT TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA
    GCAGAATCTG GTAACATAAG TCAAAAATCT GATGAAGAAG ACTTTGTGAA AGTTGAAGAT
    TTACCACTTA AACTGACAAT ATATTCAGAG GCAGATCTAA GGAAGAAAAT GGCCGAAGAA
    GAACAGAAAA ACCACTTATC TGGTGAAATA TGTGCAATGC AGACTGAAGA ATTAGCTGGG
    AATTCTCAGA CACTAAAAGA ACCTGAAACA GTGGGAGCCC AAAGTACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818589.1
    CDS461..6631
    Translation

    Target ORF information:

    RefSeq Version XM_010820287.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820287.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGATTGTCG ATACAATAGA
    GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGATG AGGAGGAGGA GGAGGAGGAA
    GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT
    GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG
    AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA
    GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC
    ACTAGAGGAA ATGCCAATAA AACACAAAAA GATGCTAGAG TAAATGAAAA GGCAAGAGAG
    AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA
    AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC
    CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT
    ACTTCATCCC CAACCGTTAA TGAGAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT
    CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA
    AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG
    AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT
    GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT
    TCTACCCAGT GTGCCCTAGA TGAAGAAGGG GATGAAGATG GTTACCTTTC TGAAGGAATT
    ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT
    CATATGTATC CTCCAAACAG TGCAAATCGT AATTCTTACA ATGTAAAGGA AACTAAAAAT
    AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA
    AGACAACAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT
    TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT
    TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA
    CAAACTCTTC TCACAAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG
    CACCTTATAA TGCACCAGTT GAACCAGTGC TATACCCAGC TAACGTGGCA GCAGAATAAT
    GTACAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCACC AACAAAATCA GCATCCAGAA
    AAACCTGCAA GCAAGGAAGG AGGCAGTGGT GCATCACACC CTCCTTCTCC CAGTTTATTT
    TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG ATTTACTAAT
    TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT
    TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA
    GGGAAAACAG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCA
    GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG
    TTTTATGACC AAGAAGGTGA GGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT
    GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC
    CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA
    ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA
    GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTG CTCAGCCGAA AAGCAGAGTG
    AAAAATATTG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA
    AACAGACATT CAGCCCAGAC CGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAA
    CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGAAACC
    AGTAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG
    TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG
    CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC
    AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT
    ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT
    GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT
    GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGTGACACT
    GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ACACGCATAT GAAGATGGAG
    GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCG TTGAGGATGA CGATGGTGCT
    GCTGCCACGA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT
    TCCCAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA
    CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG
    GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT
    CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA
    TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT
    CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT
    TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA
    GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT
    GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT
    CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TATTTCTGAT
    CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAGTTTGTC CAAAGCTGAA
    ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAGATAGAA
    GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA
    GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC
    AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG GTGACCAGGA TAGCACTCCT
    GTGAAGCCAT GTTACCTCAA TATCGTGGAA AATGAGCAAC CTTTAAATAG CCCTGTCCAG
    AAGGACACTC TCACTACCAT CGACTCGTCC AGTCAACCTA ACCCTTTGCC TTTACCTTTA
    ACTGAAATTG AAACCTTGGT GCCTAGAGTC AAAGAAGTGA AATCTGCTCA GGAGACTCCT
    GAAAGTTCTT TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA
    GCAGAATCTG GTAACATAAG TCAAAAATCT GATGAAGAAG ACTTTGTGAA AGTTGAAGAT
    TTACCACTTA AACTGACAAT ATATTCAGAG GCAGATCTAA GGAAGAAAAT GGCCGAAGAA
    GAACAGAAAA ACCACTTATC TGGTGAAATA TGTGCAATGC AGACTGAAGA ATTAGCTGGG
    AATTCTCAGA CACTAAAAGA ACCTGAAACA GTGGGAGCCC AAAGTACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50993
    Clone ID Related Accession (Same CDS sequence) XM_010820287.2 , XM_010826091.2 , XM_010820287.3
    Accession Version XM_010826091.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646718.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826091.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGATTGTCG ATACAATAGA
    GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGATG AGGAGGAGGA GGAGGAGGAA
    GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT
    GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG
    AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA
    GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC
    ACTAGAGGAA ATGCCAATAA AACACAAAAA GATGCTAGAG TAAATGAAAA GGCAAGAGAG
    AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA
    AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC
    CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT
    ACTTCATCCC CAACCGTTAA TGAGAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT
    CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA
    AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG
    AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT
    GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT
    TCTACCCAGT GTGCCCTAGA TGAAGAAGGG GATGAAGATG GTTACCTTTC TGAAGGAATT
    ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT
    CATATGTATC CTCCAAACAG TGCAAATCGT AATTCTTACA ATGTAAAGGA AACTAAAAAT
    AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA
    AGACAACAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT
    TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT
    TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA
    CAAACTCTTC TCACAAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG
    CACCTTATAA TGCACCAGTT GAACCAGTGC TATACCCAGC TAACGTGGCA GCAGAATAAT
    GTACAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCACC AACAAAATCA GCATCCAGAA
    AAACCTGCAA GCAAGGAAGG AGGCAGTGGT GCATCACACC CTCCTTCTCC CAGTTTATTT
    TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG ATTTACTAAT
    TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT
    TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA
    GGGAAAACAG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCA
    GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG
    TTTTATGACC AAGAAGGTGA GGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT
    GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC
    CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA
    ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA
    GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTG CTCAGCCGAA AAGCAGAGTG
    AAAAATATTG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA
    AACAGACATT CAGCCCAGAC CGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAA
    CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGAAACC
    AGTAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG
    TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG
    CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC
    AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT
    ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT
    GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT
    GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGTGACACT
    GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ACACGCATAT GAAGATGGAG
    GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCG TTGAGGATGA CGATGGTGCT
    GCTGCCACGA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT
    TCCCAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA
    CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG
    GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT
    CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA
    TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT
    CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT
    TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA
    GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT
    GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT
    CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TATTTCTGAT
    CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAGTTTGTC CAAAGCTGAA
    ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAGATAGAA
    GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA
    GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC
    AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG GTGACCAGGA TAGCACTCCT
    GTGAAGCCAT GTTACCTCAA TATCGTGGAA AATGAGCAAC CTTTAAATAG CCCTGTCCAG
    AAGGACACTC TCACTACCAT CGACTCGTCC AGTCAACCTA ACCCTTTGCC TTTACCTTTA
    ACTGAAATTG AAACCTTGGT GCCTAGAGTC AAAGAAGTGA AATCTGCTCA GGAGACTCCT
    GAAAGTTCTT TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA
    GCAGAATCTG GTAACATAAG TCAAAAATCT GATGAAGAAG ACTTTGTGAA AGTTGAAGAT
    TTACCACTTA AACTGACAAT ATATTCAGAG GCAGATCTAA GGAAGAAAAT GGCCGAAGAA
    GAACAGAAAA ACCACTTATC TGGTGAAATA TGTGCAATGC AGACTGAAGA ATTAGCTGGG
    AATTCTCAGA CACTAAAAGA ACCTGAAACA GTGGGAGCCC AAAGTACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824393.1
    CDS461..6631
    Translation

    Target ORF information:

    RefSeq Version XM_010826091.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826091.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGATTGTCG ATACAATAGA
    GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGATG AGGAGGAGGA GGAGGAGGAA
    GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT
    GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG
    AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA
    GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC
    ACTAGAGGAA ATGCCAATAA AACACAAAAA GATGCTAGAG TAAATGAAAA GGCAAGAGAG
    AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA
    AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC
    CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT
    ACTTCATCCC CAACCGTTAA TGAGAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT
    CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA
    AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG
    AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT
    GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT
    TCTACCCAGT GTGCCCTAGA TGAAGAAGGG GATGAAGATG GTTACCTTTC TGAAGGAATT
    ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT
    CATATGTATC CTCCAAACAG TGCAAATCGT AATTCTTACA ATGTAAAGGA AACTAAAAAT
    AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA
    AGACAACAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT
    TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT
    TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA
    CAAACTCTTC TCACAAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG
    CACCTTATAA TGCACCAGTT GAACCAGTGC TATACCCAGC TAACGTGGCA GCAGAATAAT
    GTACAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCACC AACAAAATCA GCATCCAGAA
    AAACCTGCAA GCAAGGAAGG AGGCAGTGGT GCATCACACC CTCCTTCTCC CAGTTTATTT
    TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG ATTTACTAAT
    TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT
    TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA
    GGGAAAACAG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCA
    GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG
    TTTTATGACC AAGAAGGTGA GGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT
    GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC
    CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA
    ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA
    GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTG CTCAGCCGAA AAGCAGAGTG
    AAAAATATTG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA
    AACAGACATT CAGCCCAGAC CGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAA
    CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGAAACC
    AGTAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG
    TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG
    CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC
    AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT
    ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT
    GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT
    GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGTGACACT
    GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ACACGCATAT GAAGATGGAG
    GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCG TTGAGGATGA CGATGGTGCT
    GCTGCCACGA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT
    TCCCAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA
    CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG
    GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT
    CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA
    TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT
    CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT
    TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA
    GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT
    GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT
    CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TATTTCTGAT
    CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAGTTTGTC CAAAGCTGAA
    ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAGATAGAA
    GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA
    GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC
    AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG GTGACCAGGA TAGCACTCCT
    GTGAAGCCAT GTTACCTCAA TATCGTGGAA AATGAGCAAC CTTTAAATAG CCCTGTCCAG
    AAGGACACTC TCACTACCAT CGACTCGTCC AGTCAACCTA ACCCTTTGCC TTTACCTTTA
    ACTGAAATTG AAACCTTGGT GCCTAGAGTC AAAGAAGTGA AATCTGCTCA GGAGACTCCT
    GAAAGTTCTT TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA
    GCAGAATCTG GTAACATAAG TCAAAAATCT GATGAAGAAG ACTTTGTGAA AGTTGAAGAT
    TTACCACTTA AACTGACAAT ATATTCAGAG GCAGATCTAA GGAAGAAAAT GGCCGAAGAA
    GAACAGAAAA ACCACTTATC TGGTGAAATA TGTGCAATGC AGACTGAAGA ATTAGCTGGG
    AATTCTCAGA CACTAAAAGA ACCTGAAACA GTGGGAGCCC AAAGTACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50993
    Clone ID Related Accession (Same CDS sequence) XM_010820287.2 , XM_010826091.2 , XM_010820287.3
    Accession Version XM_010820287.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pericentriolar material 1 protein isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820287.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGATTGTCG ATACAATAGA
    GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGATG AGGAGGAGGA GGAGGAGGAA
    GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT
    GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG
    AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA
    GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC
    ACTAGAGGAA ATGCCAATAA AACACAAAAA GATGCTAGAG TAAATGAAAA GGCAAGAGAG
    AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA
    AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC
    CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT
    ACTTCATCCC CAACCGTTAA TGAGAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT
    CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA
    AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG
    AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT
    GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT
    TCTACCCAGT GTGCCCTAGA TGAAGAAGGG GATGAAGATG GTTACCTTTC TGAAGGAATT
    ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT
    CATATGTATC CTCCAAACAG TGCAAATCGT AATTCTTACA ATGTAAAGGA AACTAAAAAT
    AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA
    AGACAACAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT
    TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT
    TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA
    CAAACTCTTC TCACAAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG
    CACCTTATAA TGCACCAGTT GAACCAGTGC TATACCCAGC TAACGTGGCA GCAGAATAAT
    GTACAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCACC AACAAAATCA GCATCCAGAA
    AAACCTGCAA GCAAGGAAGG AGGCAGTGGT GCATCACACC CTCCTTCTCC CAGTTTATTT
    TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG ATTTACTAAT
    TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT
    TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA
    GGGAAAACAG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCA
    GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG
    TTTTATGACC AAGAAGGTGA GGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT
    GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC
    CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA
    ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA
    GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTG CTCAGCCGAA AAGCAGAGTG
    AAAAATATTG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA
    AACAGACATT CAGCCCAGAC CGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAA
    CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGAAACC
    AGTAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG
    TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG
    CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC
    AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT
    ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT
    GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT
    GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGTGACACT
    GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ACACGCATAT GAAGATGGAG
    GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCG TTGAGGATGA CGATGGTGCT
    GCTGCCACGA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT
    TCCCAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA
    CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG
    GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT
    CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA
    TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT
    CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT
    TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA
    GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT
    GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT
    CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TATTTCTGAT
    CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAGTTTGTC CAAAGCTGAA
    ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAGATAGAA
    GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA
    GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC
    AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG GTGACCAGGA TAGCACTCCT
    GTGAAGCCAT GTTACCTCAA TATCGTGGAA AATGAGCAAC CTTTAAATAG CCCTGTCCAG
    AAGGACACTC TCACTACCAT CGACTCGTCC AGTCAACCTA ACCCTTTGCC TTTACCTTTA
    ACTGAAATTG AAACCTTGGT GCCTAGAGTC AAAGAAGTGA AATCTGCTCA GGAGACTCCT
    GAAAGTTCTT TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA
    GCAGAATCTG GTAACATAAG TCAAAAATCT GATGAAGAAG ACTTTGTGAA AGTTGAAGAT
    TTACCACTTA AACTGACAAT ATATTCAGAG GCAGATCTAA GGAAGAAAAT GGCCGAAGAA
    GAACAGAAAA ACCACTTATC TGGTGAAATA TGTGCAATGC AGACTGAAGA ATTAGCTGGG
    AATTCTCAGA CACTAAAAGA ACCTGAAACA GTGGGAGCCC AAAGTACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818589.1
    CDS359..6529
    Translation

    Target ORF information:

    RefSeq Version XM_010820287.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pericentriolar material 1 (PCM1), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820287.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG GAGGAGCTCC TTTTGAAGAA GGTGTGAATG ATCAGGATTT ACCAAACTGG 
    AGCAATGAGA GTGTTGATGA CCGACTCAAC AACATGGATT GGGGCAGCCA GCAGAAGAAG
    GCAAATAGGT CATCAGAAAA GAATAAGAGA AAATTTGGTG TAGAAAGTGA TAAAAGGGTA
    ACTAATGATA TTTCTCCGGA GTCATCGCCA GGAGTTGGAA GACGAAGAAC AAAGACTTCA
    CTGACGTTTC CACATAGTAG ATACATGACT CAGATGTCTG TTCCTGAGCA GGCAGAACTA
    GAAAAACTCA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGTGAT
    TCTCAAGGTA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC
    TTCAACTTTT TGCCTATGCA GATTAATACT AACAAGAGCA AAGATGCCAC TGTAAGTCCT
    CAAAAGAGAG AAATGATTGG ATCAGCACAG TGTAAAGAGT TGTTTGCTTC TGCTTTAAGT
    AATGACCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA ACCTGCAATG
    GAGAGCAGCC AGATTGTAAG CAGGCTTGTT CAGATTCGTG AGTATATTAC TAAAGCTAGT
    TCCATGCGGG AAGACCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTCACT
    CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT CCAAAAGATT
    CTTGCCAGAG ATCCTCAGCA GGAGCCTATA GAAGAGATAG AAAATTTGAA GAAACAACAT
    GATTTATTAA AAAGGATGTT ACAGCAGCAG GAGCAGCTGA GAGCTCTACA GGGACGACAA
    GCTGCTCTGC TTGCCCTGCA GCATAAAGCC GAGCAGGCTA TAGCTGTGAT GGATGATTCT
    GAAAAGGTTG CGGAGACAAC TCCTGGCCAT CATACTGTAC CAGGCAGACA GCCGGCTCGT
    TCACCTTTGC ATCAGAGAGT ACCCATAAGA GAAACTACAG GTAGCCTATC TGGAGTCAGT
    ATCACCTCTG AACTAAATGA AGAATTGAAT GATTTAATTC AGCATTTTCA TAATCAGCTT
    CGTGATTCTC AGCCTCCAGC TGTTCCAGAC AATAGAAGAC AAGCAGAAAG TCTTTCATTA
    ACTAGGGAGA TTTCTCAGAG CAGAAATCCA TCAGTTTCAG AGCATTTACC TGATGAGAAA
    GTCCAACTTT TTAGCAAGAT GAGAGTGTTA CAAGAAAAGA AACAAAAAAT GGACAAACTT
    CTTGGAGAAC TTCATACACT TCGAGATCAG CATTTGAACA ATTCATCATT TGTGCCTTCT
    TCAGCCTCTC CACAAAGGAG TGTGGATCAG AGAAGTACAA CCACAGCTCC TTCTGCTCCT
    GTAGTCTTAA CACCAGTGGT CAATGGAGAA TCCAATAACC TCACGCCATC TGTTCCTTAT
    CCTGCTGCTT CTCTAGTTTC TCAGAATCAG AATGAAAATG AAGGCCACCT AAATCCAACT
    GAAAAACTCC AGAAGTTAAA TGAAGTTCGA AAGAGGTTGA ATGAACTAAG AGAATTAGTT
    CATTATTATG AGCAAACATC AGATATGATG ACAGATGCTG TGAATGAAAA CACAAAAGAA
    GAAGAAACTG AAGAGTCGGA ATATGATTCT GAGCATGAAA ACTCTGAACC TGTTACTCAT
    ATTCGAAATC CACAAGTGGC TGCCACCTGG AACGAAGTAA ATAGTAATTC TAATGCACAG
    TGTGTTTCTA ACAATAGAGA TGGGCGAGCA GTTAATTCTA ACTGTGAAAT TAACAACAGG
    TCTGCTGCCA ACATAAGGGC TCTAAACATG CCTCCTCCTT CAGATTGTCG ATACAATAGA
    GAAGGAGAAC AGGAGATGCC TGTTGCACAA GGTCATGATG AGGAGGAGGA GGAGGAGGAA
    GCAGAAGATG AGGCCCTCAG TGGAGCTTCA CTGTCTAGTC ACAGCAGCAG CCTGATCATT
    GAGACTCCTG AAGATGCTGA ATTTGAACAT AAGATCCAGA AGCTTATGGC TGCAAAACTG
    AAACTTAGAC ACTTACAGAA CCTTGTAGCC ATGGTGCAGG ACGATGATGC AGCCAATCAA
    GGAGTTACTT CTGTGGAGGA TTTCTTCCCA GCAGAAGACA CCAAACAAAA TGCAAATAAC
    ACTAGAGGAA ATGCCAATAA AACACAAAAA GATGCTAGAG TAAATGAAAA GGCAAGAGAG
    AAATTTTATG AGGCTAAACT ACAGCAGCAA CAGAGAGAAC TCAAACAATT GCAGGAAGAA
    AGAAAGAAAC TGATTGAGAT TCAAGAGAAA ATTCAAGAAT TGCAAAAGGC ATGTCCTAAC
    CTACAGCTGT CAGCTGCTAG TGTGGGTAAT TGTCCCACAA AAACACATAT GCCAGCTGTT
    ACTTCATCCC CAACCGTTAA TGAGAATGAA GCCAGTACAA ACAAATCTGG TTTTGAGCCT
    CATGATTCTT CAGTGGTAGA TAATGAGGTA TGGTCAGATA TGCGAAGACA TGAAATGTTA
    AGGGAGGAGC TACGACAGAG AAGAAAGCAG CTGGAAGCTC TGATGGCTGA ACATCAGAGG
    AGGCAAGGTC TGGCTGAAAC TGCATCTCCA GTGGCTGTTT CACTGAGAAG TGATGGATCT
    GAGAACCTGT GTACACCTCA GCAAAGCAGA ACAGAAAAAA CAATGGCAAC TTGGGGAGGT
    TCTACCCAGT GTGCCCTAGA TGAAGAAGGG GATGAAGATG GTTACCTTTC TGAAGGAATT
    ATTCGGACAG ACGAAGAGGA GGAAGAGGAG GAGCAAGATG CCAGTTCCAA TGATAACTTT
    CATATGTATC CTCCAAACAG TGCAAATCGT AATTCTTACA ATGTAAAGGA AACTAAAAAT
    AGGTGGAAGA ACAATCGCCC TTTTTCAGCA GATGGAAATT ATCGTCCTTT AGCCAAGACA
    AGACAACAGA ATATCAGCAT GCAGCGTCAG GAAAACCTTC GCTGGGTGTC CGAACTCTCT
    TATGTAGAAG AGAAAGAACA ATGGCAAGAA CAAATTAATC AGCTAAAGAA ACAACTTGAT
    TTCAGTGTCA ATATTTGTCA GACTTTAATG CAAGACCAGC AGACTCTGTC TTGTCTGCTA
    CAAACTCTTC TCACAAGTCC TTATAGTGTT ATGCCCAGCA GTGTTGCATC CCCGCAAGTG
    CACCTTATAA TGCACCAGTT GAACCAGTGC TATACCCAGC TAACGTGGCA GCAGAATAAT
    GTACAGAGGT TGAAACAAAT GCTCAGTGAA CTCATGCACC AACAAAATCA GCATCCAGAA
    AAACCTGCAA GCAAGGAAGG AGGCAGTGGT GCATCACACC CTCCTTCTCC CAGTTTATTT
    TGTCCTTTCA ACTTTCCAAC TCAGCCTGTA AATCTGTTTA ACCTACCGGG ATTTACTAAT
    TTTTCGTCAT TTGCACCAAG TATGAATTTC AGCCCTTTAT TTCCTTCTAA TTTTGGAGAT
    TTTTCTCAAA ATATTTCTAC ACCCACTGAA CAGCAGCAAC CGTTAGCCCA GAATCCTTCA
    GGGAAAACAG AATACATGGC TTTTCCAAAA CCTTTTGAAA GTAGTTCTTC TATTGGAGCA
    GAAAAACAAA GGAATCAAAA ACAGCCTGAA GAGGAGGTGG AGAACAGTAG GACACCATGG
    TTTTATGACC AAGAAGGTGA GGTAGAAAAG CCATTTCTCA AGACTGGATT TGCAGTGTCT
    GTAGAGAAAA CAACAAATAG TAATCGCAAA AATCAACTAG ATACCAGTAG GAGAAGACGC
    CAGTTTGATG AAGTGTCCTT AGAAAGCTTT AACAGTATGC TTGACCCAGT AGATCCAACA
    ACAGTAACTA AAACATTCAA GTCAAGAAAA GCATCTGCAC AGGCCAGCCT GGCATCTAAA
    GATAAAACTC CCAAATCAAA GAGTAAGAAG AGGCATTCTG CTCAGCCGAA AAGCAGAGTG
    AAAAATATTG GGTATGAAAG TGCCAGTGTG TCTAGCACAT GTGAACCTTG CAAAAGTAGA
    AACAGACATT CAGCCCAGAC CGAAGAGCCT GTTCAAGCAA AAGTACTCAG CAGAAAGAAA
    CATGAGCAGC TGGAAAAAGT CATAAGATGT AGTAGGTCTA CAGAAATATC TTCAGAAACC
    AGTAGTGATT TTTCCATGTT CGAAGCTTTG CGGGATACCA TTTATTCTGA AGTAGCTACG
    TTGATTTCTC AAAATGAATC TCGTCCACAT TTTCTTATTG AACTCTTCCA TGAGCTTCAG
    CTACTTAATA CAGACTATTT GAGACAGAGG GCCTTATATG CATTGCAGGA CATAGTATCC
    AGGCATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATA TAAAGTCCGT GAATTCTGGT
    ACTTGGATAG CATCCAACTC AGAACTTACT CCCAGTGAAA GCCTTGCTAC TACTGATGAT
    GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT
    GATAATGCTA GTGTATCTTC AACTTGTGAG CCTTTTGCAA CAGATGTTCT GGGTGACACT
    GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGAGACT ACACGCATAT GAAGATGGAG
    GCTGAAAGTA GTCCAGACGT CAGGTGCACC TGCAGGATCG TTGAGGATGA CGATGGTGCT
    GCTGCCACGA CCACGATTAG TAATAATTTA GAAGAAACTC CTGTTATTGA AAATCATAGT
    TCCCAGCAAC CCGTGAGTGA AGTTGCTACC GTCCCGTGTC CTAGAATTGA TACTCAGCAA
    CTGGACCGGC AAATTAAAGC AATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG
    GATGAAGTAT GTTCTTCTCA ACTTCTAACC TCAGTAAGAC GCATGGTTTT GGTCCTTACT
    CAGCAAAACA ATGAGAGCAA AGAGTTTGTA AAGTTCTTTC ATAAGCAGCT TGGAAGTATA
    TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AGGATTGTGG AGAAGATCTT
    CTTGTAGAGA TATCTGAAGT GTTATTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT
    TTGGATAATA ATAGTGTAAC TGTTAAACAG AGATGCAAAA GGAAAATAGA AGCTGCTGGA
    GTGATACAGT CTTATGCAAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA CAGTTCACCT
    GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAGA CGGAAACAGT TAAGCAGACT
    CAAACGTCTG AGGTTTATGA TGGTGACGGC CCCAAAAATG TGAGATCTGA TATTTCTGAT
    CAAGAGGATG ATGAAGAAAG TGAAGAATGT CCAGTGTCTA TTAGTTTGTC CAAAGCTGAA
    ACTCAGGCTT TGACTAATTA CGGAAGTGGA GAAGATGAGA ATGAAGATGA AGAGATAGAA
    GAATTTGAAG AGGGACCTGT AGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAACTACA
    GAAGAAAATG AACACGATGA TCAGGTTCCA CAGCATGATT TTGAAAAATC ATCAGAAAGC
    AAAAATGTCC CATCAGAACA AGAACCCACT ACTAGTAAAG GTGACCAGGA TAGCACTCCT
    GTGAAGCCAT GTTACCTCAA TATCGTGGAA AATGAGCAAC CTTTAAATAG CCCTGTCCAG
    AAGGACACTC TCACTACCAT CGACTCGTCC AGTCAACCTA ACCCTTTGCC TTTACCTTTA
    ACTGAAATTG AAACCTTGGT GCCTAGAGTC AAAGAAGTGA AATCTGCTCA GGAGACTCCT
    GAAAGTTCTT TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA
    GCAGAATCTG GTAACATAAG TCAAAAATCT GATGAAGAAG ACTTTGTGAA AGTTGAAGAT
    TTACCACTTA AACTGACAAT ATATTCAGAG GCAGATCTAA GGAAGAAAAT GGCCGAAGAA
    GAACAGAAAA ACCACTTATC TGGTGAAATA TGTGCAATGC AGACTGAAGA ATTAGCTGGG
    AATTCTCAGA CACTAAAAGA ACCTGAAACA GTGGGAGCCC AAAGTACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.