PCM1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCM1
Entrez Gene ID	475618
Full Name	pericentriolar material 1
General protein information	Preferred Names pericentriolar material 1 Names pericentriolar material 1 protein
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005629946.2	XP_005630003.1	pericentriolar material 1 protein isoform X1
XM_005629950.1	XP_005630007.1	pericentriolar material 1 protein isoform X1
XM_005629954.2	XP_005630011.1	pericentriolar material 1 protein isoform X7
XM_005629955.1	XP_005630012.1	pericentriolar material 1 protein isoform X8
XM_022404143.1	XP_022259851.1	pericentriolar material 1 protein isoform X1
XM_022404151.1	XP_022259859.1	pericentriolar material 1 protein isoform X7
XM_022404156.1	XP_022259864.1	pericentriolar material 1 protein isoform X12
XM_022404161.1	XP_022259869.1	pericentriolar material 1 protein isoform X17
XM_022404165.1	XP_022259873.1	pericentriolar material 1 protein isoform X20
XM_014120142.1	XP_013975617.1	pericentriolar material 1 protein isoform X5
XM_005629953.1	XP_005630010.1	pericentriolar material 1 protein isoform X6
XM_005629956.2	XP_005630013.1	pericentriolar material 1 protein isoform X9
XM_022404157.1	XP_022259865.1	pericentriolar material 1 protein isoform X13
XM_022404166.1	XP_022259874.1	pericentriolar material 1 protein isoform X21
XM_014120146.1	XP_013975621.1	pericentriolar material 1 protein isoform X12
XM_005629947.1	XP_005630004.1	pericentriolar material 1 protein isoform X1
XM_005629948.1	XP_005630005.1	pericentriolar material 1 protein isoform X1
XM_005629949.1	XP_005630006.1	pericentriolar material 1 protein isoform X1
XM_005629952.2	XP_005630009.1	pericentriolar material 1 protein isoform X4
XM_005629948.2	XP_005630005.1	pericentriolar material 1 protein isoform X1
XM_005629947.2	XP_005630004.1	pericentriolar material 1 protein isoform X1
XM_022404145.1	XP_022259853.1	pericentriolar material 1 protein isoform X1
XM_022404167.1	XP_022259875.1	pericentriolar material 1 protein isoform X22
XM_005629945.1	XP_005630002.1	pericentriolar material 1 protein isoform X1
XM_014120140.1	XP_013975615.1	pericentriolar material 1 protein isoform X1
XM_014120141.1	XP_013975616.1	pericentriolar material 1 protein isoform X2
XM_005629951.1	XP_005630008.1	pericentriolar material 1 protein isoform X3
XM_014120145.1	XP_013975620.1	pericentriolar material 1 protein isoform X11
XM_005629945.2	XP_005630002.1	pericentriolar material 1 protein isoform X1
XM_005629950.2	XP_005630007.1	pericentriolar material 1 protein isoform X1
XM_022404146.1	XP_022259854.1	pericentriolar material 1 protein isoform X2
XM_022404148.1	XP_022259856.1	pericentriolar material 1 protein isoform X4
XM_022404149.1	XP_022259857.1	pericentriolar material 1 protein isoform X5
XM_022404152.1	XP_022259860.1	pericentriolar material 1 protein isoform X8
XM_022404159.1	XP_022259867.1	pericentriolar material 1 protein isoform X15
XM_022404160.1	XP_022259868.1	pericentriolar material 1 protein isoform X16
XM_022404164.1	XP_022259872.1	pericentriolar material 1 protein isoform X19
XM_014120144.1	XP_013975619.1	pericentriolar material 1 protein isoform X10
XM_022404147.1	XP_022259855.1	pericentriolar material 1 protein isoform X3
XM_022404150.1	XP_022259858.1	pericentriolar material 1 protein isoform X6
XM_022404153.1	XP_022259861.1	pericentriolar material 1 protein isoform X9
XM_022404154.1	XP_022259862.1	pericentriolar material 1 protein isoform X10
XM_022404155.1	XP_022259863.1	pericentriolar material 1 protein isoform X11
XM_022404158.1	XP_022259866.1	pericentriolar material 1 protein isoform X14
XM_022404163.1	XP_022259871.1	pericentriolar material 1 protein isoform X18

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	pcm1	NP_001120252.1
	PCM1	XP_001097950.2
Rattus norvegicus (Norway rat)	Pcm1	NP_112338.1
Pan troglodytes (chimpanzee)	PCM1	XP_001143666.2
Gallus gallus (chicken)	PCM1	NP_989862.1
Homo sapiens (human)	PCM1	NP_006188.3
Canis lupus familiaris (dog)	PCM1	XP_005630005.1
Bos taurus (cattle)	PCM1	XP_005226076.1
Mus musculus (house mouse)	Pcm1	NP_076151.2
Danio rerio (zebrafish)	pcm1	NP_001108595.1

Gene Ontology

The following PCM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe294052	XM_022404158.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe294053	XM_022404159.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe294054	XM_022404160.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe294059	XM_022404167.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe40627	XM_022404166.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe40627	XM_014120146.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe294050	XM_022404153.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe294051	XM_022404154.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe294055	XM_022404161.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe294056	XM_022404163.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe294057	XM_022404164.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe294058	XM_022404165.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe40623	XM_014120141.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe40623	XM_022404146.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe40624	XM_022404149.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe40624	XM_014120142.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe40625	XM_022404156.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe40625	XM_014120144.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe40626	XM_014120145.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe40626	XM_022404157.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe18436	XM_005629946.2 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18436	XM_022404145.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18436	XM_014120140.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18436	XM_022404143.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18436	XM_005629945.2 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18436	XM_005629949.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18436	XM_005629947.2 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18436	XM_005629950.2 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18436	XM_005629948.2 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18437	XM_005629951.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18437	XM_022404147.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18438	XM_005629952.2 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18438	XM_022404148.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18439	XM_022404150.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18439	XM_005629953.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18440	XM_022404151.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18440	XM_005629954.2 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18441	XM_022404152.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18441	XM_005629955.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18442	XM_022404155.1 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18442	XM_005629956.2 Latest version!	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OCe18436	XM_005629948.1	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18436	XM_005629947.1	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18436	XM_005629945.1	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe18436	XM_005629950.1	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCM1 ( XM_022404158.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259866.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X19, mRNA.

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CloneID	OCe294052
Clone ID Related Accession (Same CDS sequence)	XM_022404158.1
Accession Version	XM_022404158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGATCCTC AGATGGATAG AATCGCTGAT TTACATACTG 
GTCATTGGGA GGAAAAAAAC AAGACTTTCA GAATTTCCTC AGATTTTGGT AGGTCCTTCC 
AAAATACAAA GATCAGAGAT AATTTATTCA TATAGTCCTT TCCTAAAACT TTATCAAATA 
ATTAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259866.1
CDS	434..5902
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKILRWIESLIYILVIGRKKTRLSEFPQILVGPSKIQRSEIIYSYSPFLKLYQIIK

Target ORF information:

RefSeq Version	XM_022404158.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404158.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGATCCTC AGATGGATAG AATCGCTGAT TTACATACTG 
GTCATTGGGA GGAAAAAAAC AAGACTTTCA GAATTTCCTC AGATTTTGGT AGGTCCTTCC 
AAAATACAAA GATCAGAGAT AATTTATTCA TATAGTCCTT TCCTAAAACT TTATCAAATA 
ATTAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404159.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259867.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X20, mRNA.

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CloneID	OCe294053
Clone ID Related Accession (Same CDS sequence)	XM_022404159.1
Accession Version	XM_022404159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGATCCTC AGATGGATAG AATCGCTGAT TTACATACTG 
GTCATTGGGA GGAAAAAAAC AAGACTTTCA GAATTTCCTC AGATTTTGTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259867.1
CDS	434..5827
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKILRWIESLIYILVIGRKKTRLSEFPQILF

Target ORF information:

RefSeq Version	XM_022404159.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404159.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGATCCTC AGATGGATAG AATCGCTGAT TTACATACTG 
GTCATTGGGA GGAAAAAAAC AAGACTTTCA GAATTTCCTC AGATTTTGTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404160.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259868.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X21, mRNA.

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CloneID	OCe294054
Clone ID Related Accession (Same CDS sequence)	XM_022404160.1
Accession Version	XM_022404160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259868.1
CDS	434..5743
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKF

Target ORF information:

RefSeq Version	XM_022404160.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404160.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404167.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259875.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X27, mRNA.

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CloneID	OCe294059
Clone ID Related Accession (Same CDS sequence)	XM_022404167.1
Accession Version	XM_022404167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGATTGTCAC TATAATAGAG AAGGAGAACA GGGGATTCAT 
GGTGCACAAG GTGAAGACGA TGAGGAAGAA GAAGAAGCAG AAGATGAAGG AGTCAGTGGG 
GCTTCATTAA CTAGTCATAG GAGCAGTCTG GTTGATGAGG CTGCAGAAGA CGCTGAATTT 
GAACAGAAGA TCAATAGACT TATGGCTGCC AAACAGAAAT TAAGACAGTT ACAAGATCTT 
GTTGCTATGG TACAGGATGA TGATGCAGCA GATCATGGAG TTATCTCTGC CAATACTTCA 
AATTTGGATG ATTTTTACCC AGCAGAAGAA GACAACAAAC AAAGTGCAAA TAATACTAGA 
GGAAATGCCA ATAAAACACA GAAAGATGCT GGAATAAATG AGAAGGCAAG AGAGAAGTTT 
TATGAGGCTA AACTACAGCA GCAACAGAGA GAGCTCAGAC AATTGCAGGA AGAAAGAAAG 
AAACTGATTG AGATTCAAGA GAAAATTCAA GCATTACAGA AGGCATGCCC TGACTTACAG 
CTGTCAGCCA CTAGTGCGGG TAATTGTCCC ACAAAAAAAT ATATCCCAGC TGTTACTTCA 
ACCCCAGTTG TTAATGGGAA TGAAACCAGT ACAAGCAAAT CTGCTTTTGA GCCTGCAGAT 
CCTTCAGGAG TGGATAATGA GCTGTGGTCA GAAATGCGAA GACATGAGAT GTTAAGGGAA 
GAGCTACGGC AGAGAAGAAA GCAGCTTGAA GCACTGATGG CTGAACATCA GAGGAGGCAA 
GGTCTAGCTG AAACTACATC CCCTCTGGCT GTGTCATTGA GAAGTGATGG ATCTGAGAAC 
CTGTGTACGC CTCAACAGAG TAGAACAGAA AAAACCATGG CAACATGGGG AGGTTCTACC 
CAGTGTGCAC TAGATGAAGA AGGAGATGAA GATGGTTACC TTTCTGAAGG AGTTGTTCGG 
ACAGATGAAG AGGAGGAAGA AGAAGAGCAA GATGCAAGTT CGAATGATAA CTTTTCTATG 
TATCCTCCTA ACAGTGCGAA TCATAACTCT TACAACATAA AAGAAACTAA GAATAGGTGG 
AAGAATAGTC GCCCTTTTAC AGCAGATGGA AATTATCGTC CTTTAGCCAA GACAAGACAG 
CAGAATATCA GCATGCAACG TCAGGAAAAC CTTCGTTGGA TGTCCGAACT CTCTTACGTA 
GAGGAGAAAG AACAATGGCA AGAACAAATT AATCAGCTGA AGAAACAACT TGATTTCAGT 
GTCAATATTT GTCAGACTTT GATGCAAGAC CAGCAGACGC TGTCTTGTCT GCTGCAAACT 
CTTCTCACAG GTCCTTACAG TGTTATGCCC AGCAATGTAG CTTCTCCTCA AGTACACCTT 
ATAATGCACC AGTTGAATCA GTGCTATACT CAGCTAACAT GGCAACAGAA TAATGTGCAA 
AGGTTGAAAC AAATGCTAAA TGAACTTATG CGCCAACAGA ATCAGCACCC AGAAAAACCT 
GGAAGCCAGG AAAGAGGCAG TAGTGCACCA CAGCCTTCTT CTCCCAGTTT ATTTTGTCCT 
TTCAGCTTTC CATCACAGCC TGTAAATCTA TTTAACCTAC CCGGGTTTAC TAATTTTTCA 
TCATTTGCAC CAGGTATGAA TTTTAGCCCT TTATTTCCTT CTAATTTTGG AGAATTTTCT 
CAGAATATTT CTACACCTAC TGAACAGCAG CAACCATTAG CCCAGAATTC TTCAGGGAAA 
ACAGAATATA TGGCTTTTCC AAAACCTTTT GAAAGCAGTT CTTCTATTGG AGCAGAAAAA 
CAAAGGAATC AAAAACAGCC TGGAGAGGAG GTGGAAAACA GTAGGACAGC GTGGTTATAT 
GACCAAGAAG GTGAAGTAGA AAAACCATTT ATCAAGACTG GATTTCCAGT GTCTGTAGAG 
AAAACTACAA ATAGTAATCG CAAAAATCAA TTAGATACCG GCCGAAGAAG ACGCCAGTTT 
GATGAAGAGT CATTAGAAAG CTTTAGCAGT ATGCCTGATC CAGTAGATCC GACAACAGTA 
ACCAAAACAT TCAAGACGAG AAAAGCATCT GCACAGGCCA GCCTAGCATC TAAAGATAAA 
ACTCCTAAAT CAAAGAGTAA GAAAAGGCAT TCTGCTCAAC TAAAAAGCAG AGTTAAAAAT 
ACTGAAACTG GGAGTGATTT TTCCATGTTT GAAGCTTTGC GGGATACTAT TTATTCTGAA 
GTAGCTACAT TAATTTCTCA AAATGAATCT CATCCACATT TTCTTATTGA ACTCTTCCAT 
GAACTTCAGC TACTTAATAC AGACTACTTG AGACAAAGGG CTTTATATGC ATTGCAGGAC 
ATAGTAACCA GACATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATGT AAAGTCAGTG 
AATTCTGGTA CTTGGATAGC ATCAAACTCA GAGCTTACCC CCAGTGAAAG CCTTGCTACC 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG CGTCATGTCT GTATCTTCAA ATTTTGAGCC TTTTGCAACA 
GATGATCTAG GTAACACTGT GATTCACTTA GATCAGGCAT TAGCCAGAAT GAGGGAATAC 
GAGCGTATGA AGACTGAGGC TGAAAGTAGC ACAAATATAA GATGCACCTG CAGAATTCTT 
GAGGATGAGG ATGGTGCTGC CGCCACAAGT ATGGTTACTA ATTTAGAAGA AACTCCTATT 
GAAAATCATG GTTCACAACA ACCTGTAAGT GAAGTTTCTA CTGTTCCATG TCCTAGAATT 
GATACTCAGC AGCTGGACCG GCAAATTAAA GCCATTATGA AAGAAGTCAT TCCTTTTTTG 
AAGGAGCACA TGGATGAAGT ATGCTCTTCT CAACTTCTAA CTTCAGTAAG GCGTATGGTT 
TTGACCCTCA CCCAACAAAA TGATGAGAGC AAAGAATTTG TGAAGTTCTT TCATAAACAA 
CTTGGAAGTA TATTACAGGA TTCACTGGCA AAATTTGCTG GCAGAAAACT GAAAGACTGT 
GGAGAAGATC TTCTTGTAGA GATATCTGAA GTGTTGTTTA ATGAATTGGC TTTCTTTAAG 
CTCATGCAAG ATTTGGATAA TAATAGTATA ACTGTTAAAC AGAAGTGCAA AAGGAAAATA 
GAAGCAGCTG GAGTGATACA GTCTTATGCC AAAGAGGCCA AAAGGATTCT TGAAGGAGAT 
CATGGCTCAC CTGCTGGAGA AATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCCGA CTCAGACATC TGAGATATAT GATGGTGATG GTCCCAAAAA TGTGAGATCA 
GATGTTTCTG ATCAAGAGGA AGATGAAGAA AGTGAAGAAT GTCCGGTGTC TATTAACTTG 
TCCAAAGCTG AAACTCAGGC TTTGACTAAT TATGGAAGTG GAGAAGATGA GAATGAGGAT 
GAAGAAATAG AAGAATTTGA AGAGGGACCT GTGGATGTCC AGACGTCACT TCAGGCTAAC 
ACTGAAGCTA CAGAAGAAAC TGAACACGAT GATCAGGTAC TACAACATGA CTTTGAAAAA 
TCAGGAGAAA GCAAAAATGT CCCATCAGAA CAAGATCCCA CTACTAGTAA AGGCAAGAAA 
TATGACCAAG ATAGTACTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AAATGAGCAG 
CCTTTGAATA GCGCTGTCCA AAAAGACTCT CTGACTACCA TCGATTCATC TAAACAGCCT 
AACCCTTTGC CTTTACCTTT ACCTGAAATC GAAACCTTGG TGCCTACAGT CAAAGAAGTT 
AAATCTGCTC AAGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA 
GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATACTCAGA GGCAGATCTA 
AGGAAGAAAA TGGTAGAAGA GCAAGAGAAA AACCATTTAT CTGGTGAAAT ACTATGTGAA 
ATGCAGACCG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA 
GCCCAAAGCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259875.1
CDS	426..6380
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLDCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404167.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404167.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGATTGTCAC TATAATAGAG AAGGAGAACA GGGGATTCAT 
GGTGCACAAG GTGAAGACGA TGAGGAAGAA GAAGAAGCAG AAGATGAAGG AGTCAGTGGG 
GCTTCATTAA CTAGTCATAG GAGCAGTCTG GTTGATGAGG CTGCAGAAGA CGCTGAATTT 
GAACAGAAGA TCAATAGACT TATGGCTGCC AAACAGAAAT TAAGACAGTT ACAAGATCTT 
GTTGCTATGG TACAGGATGA TGATGCAGCA GATCATGGAG TTATCTCTGC CAATACTTCA 
AATTTGGATG ATTTTTACCC AGCAGAAGAA GACAACAAAC AAAGTGCAAA TAATACTAGA 
GGAAATGCCA ATAAAACACA GAAAGATGCT GGAATAAATG AGAAGGCAAG AGAGAAGTTT 
TATGAGGCTA AACTACAGCA GCAACAGAGA GAGCTCAGAC AATTGCAGGA AGAAAGAAAG 
AAACTGATTG AGATTCAAGA GAAAATTCAA GCATTACAGA AGGCATGCCC TGACTTACAG 
CTGTCAGCCA CTAGTGCGGG TAATTGTCCC ACAAAAAAAT ATATCCCAGC TGTTACTTCA 
ACCCCAGTTG TTAATGGGAA TGAAACCAGT ACAAGCAAAT CTGCTTTTGA GCCTGCAGAT 
CCTTCAGGAG TGGATAATGA GCTGTGGTCA GAAATGCGAA GACATGAGAT GTTAAGGGAA 
GAGCTACGGC AGAGAAGAAA GCAGCTTGAA GCACTGATGG CTGAACATCA GAGGAGGCAA 
GGTCTAGCTG AAACTACATC CCCTCTGGCT GTGTCATTGA GAAGTGATGG ATCTGAGAAC 
CTGTGTACGC CTCAACAGAG TAGAACAGAA AAAACCATGG CAACATGGGG AGGTTCTACC 
CAGTGTGCAC TAGATGAAGA AGGAGATGAA GATGGTTACC TTTCTGAAGG AGTTGTTCGG 
ACAGATGAAG AGGAGGAAGA AGAAGAGCAA GATGCAAGTT CGAATGATAA CTTTTCTATG 
TATCCTCCTA ACAGTGCGAA TCATAACTCT TACAACATAA AAGAAACTAA GAATAGGTGG 
AAGAATAGTC GCCCTTTTAC AGCAGATGGA AATTATCGTC CTTTAGCCAA GACAAGACAG 
CAGAATATCA GCATGCAACG TCAGGAAAAC CTTCGTTGGA TGTCCGAACT CTCTTACGTA 
GAGGAGAAAG AACAATGGCA AGAACAAATT AATCAGCTGA AGAAACAACT TGATTTCAGT 
GTCAATATTT GTCAGACTTT GATGCAAGAC CAGCAGACGC TGTCTTGTCT GCTGCAAACT 
CTTCTCACAG GTCCTTACAG TGTTATGCCC AGCAATGTAG CTTCTCCTCA AGTACACCTT 
ATAATGCACC AGTTGAATCA GTGCTATACT CAGCTAACAT GGCAACAGAA TAATGTGCAA 
AGGTTGAAAC AAATGCTAAA TGAACTTATG CGCCAACAGA ATCAGCACCC AGAAAAACCT 
GGAAGCCAGG AAAGAGGCAG TAGTGCACCA CAGCCTTCTT CTCCCAGTTT ATTTTGTCCT 
TTCAGCTTTC CATCACAGCC TGTAAATCTA TTTAACCTAC CCGGGTTTAC TAATTTTTCA 
TCATTTGCAC CAGGTATGAA TTTTAGCCCT TTATTTCCTT CTAATTTTGG AGAATTTTCT 
CAGAATATTT CTACACCTAC TGAACAGCAG CAACCATTAG CCCAGAATTC TTCAGGGAAA 
ACAGAATATA TGGCTTTTCC AAAACCTTTT GAAAGCAGTT CTTCTATTGG AGCAGAAAAA 
CAAAGGAATC AAAAACAGCC TGGAGAGGAG GTGGAAAACA GTAGGACAGC GTGGTTATAT 
GACCAAGAAG GTGAAGTAGA AAAACCATTT ATCAAGACTG GATTTCCAGT GTCTGTAGAG 
AAAACTACAA ATAGTAATCG CAAAAATCAA TTAGATACCG GCCGAAGAAG ACGCCAGTTT 
GATGAAGAGT CATTAGAAAG CTTTAGCAGT ATGCCTGATC CAGTAGATCC GACAACAGTA 
ACCAAAACAT TCAAGACGAG AAAAGCATCT GCACAGGCCA GCCTAGCATC TAAAGATAAA 
ACTCCTAAAT CAAAGAGTAA GAAAAGGCAT TCTGCTCAAC TAAAAAGCAG AGTTAAAAAT 
ACTGAAACTG GGAGTGATTT TTCCATGTTT GAAGCTTTGC GGGATACTAT TTATTCTGAA 
GTAGCTACAT TAATTTCTCA AAATGAATCT CATCCACATT TTCTTATTGA ACTCTTCCAT 
GAACTTCAGC TACTTAATAC AGACTACTTG AGACAAAGGG CTTTATATGC ATTGCAGGAC 
ATAGTAACCA GACATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATGT AAAGTCAGTG 
AATTCTGGTA CTTGGATAGC ATCAAACTCA GAGCTTACCC CCAGTGAAAG CCTTGCTACC 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG CGTCATGTCT GTATCTTCAA ATTTTGAGCC TTTTGCAACA 
GATGATCTAG GTAACACTGT GATTCACTTA GATCAGGCAT TAGCCAGAAT GAGGGAATAC 
GAGCGTATGA AGACTGAGGC TGAAAGTAGC ACAAATATAA GATGCACCTG CAGAATTCTT 
GAGGATGAGG ATGGTGCTGC CGCCACAAGT ATGGTTACTA ATTTAGAAGA AACTCCTATT 
GAAAATCATG GTTCACAACA ACCTGTAAGT GAAGTTTCTA CTGTTCCATG TCCTAGAATT 
GATACTCAGC AGCTGGACCG GCAAATTAAA GCCATTATGA AAGAAGTCAT TCCTTTTTTG 
AAGGAGCACA TGGATGAAGT ATGCTCTTCT CAACTTCTAA CTTCAGTAAG GCGTATGGTT 
TTGACCCTCA CCCAACAAAA TGATGAGAGC AAAGAATTTG TGAAGTTCTT TCATAAACAA 
CTTGGAAGTA TATTACAGGA TTCACTGGCA AAATTTGCTG GCAGAAAACT GAAAGACTGT 
GGAGAAGATC TTCTTGTAGA GATATCTGAA GTGTTGTTTA ATGAATTGGC TTTCTTTAAG 
CTCATGCAAG ATTTGGATAA TAATAGTATA ACTGTTAAAC AGAAGTGCAA AAGGAAAATA 
GAAGCAGCTG GAGTGATACA GTCTTATGCC AAAGAGGCCA AAAGGATTCT TGAAGGAGAT 
CATGGCTCAC CTGCTGGAGA AATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCCGA CTCAGACATC TGAGATATAT GATGGTGATG GTCCCAAAAA TGTGAGATCA 
GATGTTTCTG ATCAAGAGGA AGATGAAGAA AGTGAAGAAT GTCCGGTGTC TATTAACTTG 
TCCAAAGCTG AAACTCAGGC TTTGACTAAT TATGGAAGTG GAGAAGATGA GAATGAGGAT 
GAAGAAATAG AAGAATTTGA AGAGGGACCT GTGGATGTCC AGACGTCACT TCAGGCTAAC 
ACTGAAGCTA CAGAAGAAAC TGAACACGAT GATCAGGTAC TACAACATGA CTTTGAAAAA 
TCAGGAGAAA GCAAAAATGT CCCATCAGAA CAAGATCCCA CTACTAGTAA AGGCAAGAAA 
TATGACCAAG ATAGTACTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AAATGAGCAG 
CCTTTGAATA GCGCTGTCCA AAAAGACTCT CTGACTACCA TCGATTCATC TAAACAGCCT 
AACCCTTTGC CTTTACCTTT ACCTGAAATC GAAACCTTGG TGCCTACAGT CAAAGAAGTT 
AAATCTGCTC AAGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA 
GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATACTCAGA GGCAGATCTA 
AGGAAGAAAA TGGTAGAAGA GCAAGAGAAA AACCATTTAT CTGGTGAAAT ACTATGTGAA 
ATGCAGACCG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA 
GCCCAAAGCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404166.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259874.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X26, mRNA.

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CloneID	OCe40627
Clone ID Related Accession (Same CDS sequence)	XM_022404166.1 , XM_014120146.1
Accession Version	XM_022404166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGCAGATTGT CACTATAATA GAGAAGGAGA ACAGGGGATT 
CATGGTGCAC AAGGTGAAGA CGATGAGGAA GAAGAAGAAG CAGAAGATGA AGGAGTCAGT 
GGGGCTTCAT TAACTAGTCA TAGGAGCAGT CTGGTTGATG AGGCTGCAGA AGACGCTGAA 
TTTGAACAGA AGATCAATAG ACTTATGGCT GCCAAACAGA AATTAAGACA GTTACAAGAT 
CTTGTTGCTA TGGTACAGGA TGATGATGCA GCAGATCATG GAGTTATCTC TGCCAATACT 
TCAAATTTGG ATGATTTTTA CCCAGCAGAA GAAGACAACA AACAAAGTGC AAATAATACT 
AGAGGAAATG CCAATAAAAC ACAGAAAGAT GCTGGAATAA ATGAGAAGGC AAGAGAGAAG 
TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAGCTCA GACAATTGCA GGAAGAAAGA 
AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGCATTAC AGAAGGCATG CCCTGACTTA 
CAGCTGTCAG CCACTAGTGC GGGTAATTGT CCCACAAAAA AATATATCCC AGCTGTTACT 
TCAACCCCAG TTGTTAATGG GAATGAAACC AGTACAAGCA AATCTGCTTT TGAGCCTGCA 
GATCCTTCAG GAGTGGATAA TGAGCTGTGG TCAGAAATGC GAAGACATGA GATGTTAAGG 
GAAGAGCTAC GGCAGAGAAG AAAGCAGCTT GAAGCACTGA TGGCTGAACA TCAGAGGAGG 
CAAGGTCTAG CTGAAACTAC ATCCCCTCTG GCTGTGTCAT TGAGAAGTGA TGGATCTGAG 
AACCTGTGTA CGCCTCAACA GAGTAGAACA GAAAAAACCA TGGCAACATG GGGAGGTTCT 
ACCCAGTGTG CACTAGATGA AGAAGGAGAT GAAGATGGTT ACCTTTCTGA AGGAGTTGTT 
CGGACAGATG AAGAGGAGGA AGAAGAAGAG CAAGATGCAA GTTCGAATGA TAACTTTTCT 
ATGTATCCTC CTAACAGTGC GAATCATAAC TCTTACAACA TAAAAGAAAC TAAGAATAGG 
TGGAAGAATA GTCGCCCTTT TACAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA 
CAGCAGAATA TCAGCATGCA ACGTCAGGAA AACCTTCGTT GGATGTCCGA ACTCTCTTAC 
GTAGAGGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TGAAGAAACA ACTTGATTTC 
AGTGTCAATA TTTGTCAGAC TTTGATGCAA GACCAGCAGA CGCTGTCTTG TCTGCTGCAA 
ACTCTTCTCA CAGGTCCTTA CAGTGTTATG CCCAGCAATG TAGCTTCTCC TCAAGTACAC 
CTTATAATGC ACCAGTTGAA TCAGTGCTAT ACTCAGCTAA CATGGCAACA GAATAATGTG 
CAAAGGTTGA AACAAATGCT AAATGAACTT ATGCGCCAAC AGAATCAGCA CCCAGAAAAA 
CCTGGAAGCC AGGAAAGAGG CAGTAGTGCA CCACAGCCTT CTTCTCCCAG TTTATTTTGT 
CCTTTCAGCT TTCCATCACA GCCTGTAAAT CTATTTAACC TACCCGGGTT TACTAATTTT 
TCATCATTTG CACCAGGTAT GAATTTTAGC CCTTTATTTC CTTCTAATTT TGGAGAATTT 
TCTCAGAATA TTTCTACACC TACTGAACAG CAGCAACCAT TAGCCCAGAA TTCTTCAGGG 
AAAACAGAAT ATATGGCTTT TCCAAAACCT TTTGAAAGCA GTTCTTCTAT TGGAGCAGAA 
AAACAAAGGA ATCAAAAACA GCCTGGAGAG GAGGTGGAAA ACAGTAGGAC AGCGTGGTTA 
TATGACCAAG AAGGTGAAGT AGAAAAACCA TTTATCAAGA CTGGATTTCC AGTGTCTGTA 
GAGAAAACTA CAAATAGTAA TCGCAAAAAT CAATTAGATA CCGGCCGAAG AAGACGCCAG 
TTTGATGAAG AGTCATTAGA AAGCTTTAGC AGTATGCCTG ATCCAGTAGA TCCGACAACA 
GTAACCAAAA CATTCAAGAC GAGAAAAGCA TCTGCACAGG CCAGCCTAGC ATCTAAAGAT 
AAAACTCCTA AATCAAAGAG TAAGAAAAGG CATTCTGCTC AACTAAAAAG CAGAGTTAAA 
AATACTGAAA CTGGGAGTGA TTTTTCCATG TTTGAAGCTT TGCGGGATAC TATTTATTCT 
GAAGTAGCTA CATTAATTTC TCAAAATGAA TCTCATCCAC ATTTTCTTAT TGAACTCTTC 
CATGAACTTC AGCTACTTAA TACAGACTAC TTGAGACAAA GGGCTTTATA TGCATTGCAG 
GACATAGTAA CCAGACATAT TTCTGAGAAC CATGAAAAAG AAGGAGAAAA TGTAAAGTCA 
GTGAATTCTG GTACTTGGAT AGCATCAAAC TCAGAGCTTA CCCCCAGTGA AAGCCTTGCT 
ACCACTGATG ATGAAACTTT TGAGAAGAAC TTTGAGAGAG AAACACATAA AATAAGTGAG 
CAAAATGATG CTGATAATGC TAGCGTCATG TCTGTATCTT CAAATTTTGA GCCTTTTGCA 
ACAGATGATC TAGGTAACAC TGTGATTCAC TTAGATCAGG CATTAGCCAG AATGAGGGAA 
TACGAGCGTA TGAAGACTGA GGCTGAAAGT AGCACAAATA TAAGATGCAC CTGCAGAATT 
CTTGAGGATG AGGATGGTGC TGCCGCCACA AGTATGGTTA CTAATTTAGA AGAAACTCCT 
ATTGAAAATC ATGGTTCACA ACAACCTGTA AGTGAAGTTT CTACTGTTCC ATGTCCTAGA 
ATTGATACTC AGCAGCTGGA CCGGCAAATT AAAGCCATTA TGAAAGAAGT CATTCCTTTT 
TTGAAGGAGC ACATGGATGA AGTATGCTCT TCTCAACTTC TAACTTCAGT AAGGCGTATG 
GTTTTGACCC TCACCCAACA AAATGATGAG AGCAAAGAAT TTGTGAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TTAATGAATT GGCTTTCTTT 
AAGCTCATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAAGTG CAAAAGGAAA 
ATAGAAGCAG CTGGAGTGAT ACAGTCTTAT GCCAAAGAGG CCAAAAGGAT TCTTGAAGGA 
GATCATGGCT CACCTGCTGG AGAAATTGAT GATGAAGACA AAGACAAGGA TGAAACTGAA 
ACAGTTAAGC CGACTCAGAC ATCTGAGATA TATGATGGTG ATGGTCCCAA AAATGTGAGA 
TCAGATGTTT CTGATCAAGA GGAAGATGAA GAAAGTGAAG AATGTCCGGT GTCTATTAAC 
TTGTCCAAAG CTGAAACTCA GGCTTTGACT AATTATGGAA GTGGAGAAGA TGAGAATGAG 
GATGAAGAAA TAGAAGAATT TGAAGAGGGA CCTGTGGATG TCCAGACGTC ACTTCAGGCT 
AACACTGAAG CTACAGAAGA AACTGAACAC GATGATCAGG TACTACAACA TGACTTTGAA 
AAATCAGGAG AAAGCAAAAA TGTCCCATCA GAACAAGATC CCACTACTAG TAAAGGCAAG 
AAATATGACC AAGATAGTAC TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAAATGAG 
CAGCCTTTGA ATAGCGCTGT CCAAAAAGAC TCTCTGACTA CCATCGATTC ATCTAAACAG 
CCTAACCCTT TGCCTTTACC TTTACCTGAA ATCGAAACCT TGGTGCCTAC AGTCAAAGAA 
GTTAAATCTG CTCAAGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA 
GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATACTC AGAGGCAGAT 
CTAAGGAAGA AAATGGTAGA AGAGCAAGAG AAAAACCATT TATCTGGTGA AATACTATGT 
GAAATGCAGA CCGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAAACAGTG 
GGAGCCCAAA GCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259874.1
CDS	426..6383
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404166.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404166.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGCAGATTGT CACTATAATA GAGAAGGAGA ACAGGGGATT 
CATGGTGCAC AAGGTGAAGA CGATGAGGAA GAAGAAGAAG CAGAAGATGA AGGAGTCAGT 
GGGGCTTCAT TAACTAGTCA TAGGAGCAGT CTGGTTGATG AGGCTGCAGA AGACGCTGAA 
TTTGAACAGA AGATCAATAG ACTTATGGCT GCCAAACAGA AATTAAGACA GTTACAAGAT 
CTTGTTGCTA TGGTACAGGA TGATGATGCA GCAGATCATG GAGTTATCTC TGCCAATACT 
TCAAATTTGG ATGATTTTTA CCCAGCAGAA GAAGACAACA AACAAAGTGC AAATAATACT 
AGAGGAAATG CCAATAAAAC ACAGAAAGAT GCTGGAATAA ATGAGAAGGC AAGAGAGAAG 
TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAGCTCA GACAATTGCA GGAAGAAAGA 
AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGCATTAC AGAAGGCATG CCCTGACTTA 
CAGCTGTCAG CCACTAGTGC GGGTAATTGT CCCACAAAAA AATATATCCC AGCTGTTACT 
TCAACCCCAG TTGTTAATGG GAATGAAACC AGTACAAGCA AATCTGCTTT TGAGCCTGCA 
GATCCTTCAG GAGTGGATAA TGAGCTGTGG TCAGAAATGC GAAGACATGA GATGTTAAGG 
GAAGAGCTAC GGCAGAGAAG AAAGCAGCTT GAAGCACTGA TGGCTGAACA TCAGAGGAGG 
CAAGGTCTAG CTGAAACTAC ATCCCCTCTG GCTGTGTCAT TGAGAAGTGA TGGATCTGAG 
AACCTGTGTA CGCCTCAACA GAGTAGAACA GAAAAAACCA TGGCAACATG GGGAGGTTCT 
ACCCAGTGTG CACTAGATGA AGAAGGAGAT GAAGATGGTT ACCTTTCTGA AGGAGTTGTT 
CGGACAGATG AAGAGGAGGA AGAAGAAGAG CAAGATGCAA GTTCGAATGA TAACTTTTCT 
ATGTATCCTC CTAACAGTGC GAATCATAAC TCTTACAACA TAAAAGAAAC TAAGAATAGG 
TGGAAGAATA GTCGCCCTTT TACAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA 
CAGCAGAATA TCAGCATGCA ACGTCAGGAA AACCTTCGTT GGATGTCCGA ACTCTCTTAC 
GTAGAGGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TGAAGAAACA ACTTGATTTC 
AGTGTCAATA TTTGTCAGAC TTTGATGCAA GACCAGCAGA CGCTGTCTTG TCTGCTGCAA 
ACTCTTCTCA CAGGTCCTTA CAGTGTTATG CCCAGCAATG TAGCTTCTCC TCAAGTACAC 
CTTATAATGC ACCAGTTGAA TCAGTGCTAT ACTCAGCTAA CATGGCAACA GAATAATGTG 
CAAAGGTTGA AACAAATGCT AAATGAACTT ATGCGCCAAC AGAATCAGCA CCCAGAAAAA 
CCTGGAAGCC AGGAAAGAGG CAGTAGTGCA CCACAGCCTT CTTCTCCCAG TTTATTTTGT 
CCTTTCAGCT TTCCATCACA GCCTGTAAAT CTATTTAACC TACCCGGGTT TACTAATTTT 
TCATCATTTG CACCAGGTAT GAATTTTAGC CCTTTATTTC CTTCTAATTT TGGAGAATTT 
TCTCAGAATA TTTCTACACC TACTGAACAG CAGCAACCAT TAGCCCAGAA TTCTTCAGGG 
AAAACAGAAT ATATGGCTTT TCCAAAACCT TTTGAAAGCA GTTCTTCTAT TGGAGCAGAA 
AAACAAAGGA ATCAAAAACA GCCTGGAGAG GAGGTGGAAA ACAGTAGGAC AGCGTGGTTA 
TATGACCAAG AAGGTGAAGT AGAAAAACCA TTTATCAAGA CTGGATTTCC AGTGTCTGTA 
GAGAAAACTA CAAATAGTAA TCGCAAAAAT CAATTAGATA CCGGCCGAAG AAGACGCCAG 
TTTGATGAAG AGTCATTAGA AAGCTTTAGC AGTATGCCTG ATCCAGTAGA TCCGACAACA 
GTAACCAAAA CATTCAAGAC GAGAAAAGCA TCTGCACAGG CCAGCCTAGC ATCTAAAGAT 
AAAACTCCTA AATCAAAGAG TAAGAAAAGG CATTCTGCTC AACTAAAAAG CAGAGTTAAA 
AATACTGAAA CTGGGAGTGA TTTTTCCATG TTTGAAGCTT TGCGGGATAC TATTTATTCT 
GAAGTAGCTA CATTAATTTC TCAAAATGAA TCTCATCCAC ATTTTCTTAT TGAACTCTTC 
CATGAACTTC AGCTACTTAA TACAGACTAC TTGAGACAAA GGGCTTTATA TGCATTGCAG 
GACATAGTAA CCAGACATAT TTCTGAGAAC CATGAAAAAG AAGGAGAAAA TGTAAAGTCA 
GTGAATTCTG GTACTTGGAT AGCATCAAAC TCAGAGCTTA CCCCCAGTGA AAGCCTTGCT 
ACCACTGATG ATGAAACTTT TGAGAAGAAC TTTGAGAGAG AAACACATAA AATAAGTGAG 
CAAAATGATG CTGATAATGC TAGCGTCATG TCTGTATCTT CAAATTTTGA GCCTTTTGCA 
ACAGATGATC TAGGTAACAC TGTGATTCAC TTAGATCAGG CATTAGCCAG AATGAGGGAA 
TACGAGCGTA TGAAGACTGA GGCTGAAAGT AGCACAAATA TAAGATGCAC CTGCAGAATT 
CTTGAGGATG AGGATGGTGC TGCCGCCACA AGTATGGTTA CTAATTTAGA AGAAACTCCT 
ATTGAAAATC ATGGTTCACA ACAACCTGTA AGTGAAGTTT CTACTGTTCC ATGTCCTAGA 
ATTGATACTC AGCAGCTGGA CCGGCAAATT AAAGCCATTA TGAAAGAAGT CATTCCTTTT 
TTGAAGGAGC ACATGGATGA AGTATGCTCT TCTCAACTTC TAACTTCAGT AAGGCGTATG 
GTTTTGACCC TCACCCAACA AAATGATGAG AGCAAAGAAT TTGTGAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TTAATGAATT GGCTTTCTTT 
AAGCTCATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAAGTG CAAAAGGAAA 
ATAGAAGCAG CTGGAGTGAT ACAGTCTTAT GCCAAAGAGG CCAAAAGGAT TCTTGAAGGA 
GATCATGGCT CACCTGCTGG AGAAATTGAT GATGAAGACA AAGACAAGGA TGAAACTGAA 
ACAGTTAAGC CGACTCAGAC ATCTGAGATA TATGATGGTG ATGGTCCCAA AAATGTGAGA 
TCAGATGTTT CTGATCAAGA GGAAGATGAA GAAAGTGAAG AATGTCCGGT GTCTATTAAC 
TTGTCCAAAG CTGAAACTCA GGCTTTGACT AATTATGGAA GTGGAGAAGA TGAGAATGAG 
GATGAAGAAA TAGAAGAATT TGAAGAGGGA CCTGTGGATG TCCAGACGTC ACTTCAGGCT 
AACACTGAAG CTACAGAAGA AACTGAACAC GATGATCAGG TACTACAACA TGACTTTGAA 
AAATCAGGAG AAAGCAAAAA TGTCCCATCA GAACAAGATC CCACTACTAG TAAAGGCAAG 
AAATATGACC AAGATAGTAC TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAAATGAG 
CAGCCTTTGA ATAGCGCTGT CCAAAAAGAC TCTCTGACTA CCATCGATTC ATCTAAACAG 
CCTAACCCTT TGCCTTTACC TTTACCTGAA ATCGAAACCT TGGTGCCTAC AGTCAAAGAA 
GTTAAATCTG CTCAAGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA 
GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATACTC AGAGGCAGAT 
CTAAGGAAGA AAATGGTAGA AGAGCAAGAG AAAAACCATT TATCTGGTGA AATACTATGT 
GAAATGCAGA CCGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAAACAGTG 
GGAGCCCAAA GCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_014120146.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975621.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X18, mRNA.

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CloneID	OCe40627
Clone ID Related Accession (Same CDS sequence)	XM_022404166.1 , XM_014120146.1
Accession Version	XM_014120146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGCAGATTGT CACTATAATA GAGAAGGAGA ACAGGGGATT 
CATGGTGCAC AAGGTGAAGA CGATGAGGAA GAAGAAGAAG CAGAAGATGA AGGAGTCAGT 
GGGGCTTCAT TAACTAGTCA TAGGAGCAGT CTGGTTGATG AGGCTGCAGA AGACGCTGAA 
TTTGAACAGA AGATCAATAG ACTTATGGCT GCCAAACAGA AATTAAGACA GTTACAAGAT 
CTTGTTGCTA TGGTACAGGA TGATGATGCA GCAGATCATG GAGTTATCTC TGCCAATACT 
TCAAATTTGG ATGATTTTTA CCCAGCAGAA GAAGACAACA AACAAAGTGC AAATAATACT 
AGAGGAAATG CCAATAAAAC ACAGAAAGAT GCTGGAATAA ATGAGAAGGC AAGAGAGAAG 
TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAGCTCA GACAATTGCA GGAAGAAAGA 
AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGCATTAC AGAAGGCATG CCCTGACTTA 
CAGCTGTCAG CCACTAGTGC GGGTAATTGT CCCACAAAAA AATATATCCC AGCTGTTACT 
TCAACCCCAG TTGTTAATGG GAATGAAACC AGTACAAGCA AATCTGCTTT TGAGCCTGCA 
GATCCTTCAG GAGTGGATAA TGAGCTGTGG TCAGAAATGC GAAGACATGA GATGTTAAGG 
GAAGAGCTAC GGCAGAGAAG AAAGCAGCTT GAAGCACTGA TGGCTGAACA TCAGAGGAGG 
CAAGGTCTAG CTGAAACTAC ATCCCCTCTG GCTGTGTCAT TGAGAAGTGA TGGATCTGAG 
AACCTGTGTA CGCCTCAACA GAGTAGAACA GAAAAAACCA TGGCAACATG GGGAGGTTCT 
ACCCAGTGTG CACTAGATGA AGAAGGAGAT GAAGATGGTT ACCTTTCTGA AGGAGTTGTT 
CGGACAGATG AAGAGGAGGA AGAAGAAGAG CAAGATGCAA GTTCGAATGA TAACTTTTCT 
ATGTATCCTC CTAACAGTGC GAATCATAAC TCTTACAACA TAAAAGAAAC TAAGAATAGG 
TGGAAGAATA GTCGCCCTTT TACAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA 
CAGCAGAATA TCAGCATGCA ACGTCAGGAA AACCTTCGTT GGATGTCCGA ACTCTCTTAC 
GTAGAGGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TGAAGAAACA ACTTGATTTC 
AGTGTCAATA TTTGTCAGAC TTTGATGCAA GACCAGCAGA CGCTGTCTTG TCTGCTGCAA 
ACTCTTCTCA CAGGTCCTTA CAGTGTTATG CCCAGCAATG TAGCTTCTCC TCAAGTACAC 
CTTATAATGC ACCAGTTGAA TCAGTGCTAT ACTCAGCTAA CATGGCAACA GAATAATGTG 
CAAAGGTTGA AACAAATGCT AAATGAACTT ATGCGCCAAC AGAATCAGCA CCCAGAAAAA 
CCTGGAAGCC AGGAAAGAGG CAGTAGTGCA CCACAGCCTT CTTCTCCCAG TTTATTTTGT 
CCTTTCAGCT TTCCATCACA GCCTGTAAAT CTATTTAACC TACCCGGGTT TACTAATTTT 
TCATCATTTG CACCAGGTAT GAATTTTAGC CCTTTATTTC CTTCTAATTT TGGAGAATTT 
TCTCAGAATA TTTCTACACC TACTGAACAG CAGCAACCAT TAGCCCAGAA TTCTTCAGGG 
AAAACAGAAT ATATGGCTTT TCCAAAACCT TTTGAAAGCA GTTCTTCTAT TGGAGCAGAA 
AAACAAAGGA ATCAAAAACA GCCTGGAGAG GAGGTGGAAA ACAGTAGGAC AGCGTGGTTA 
TATGACCAAG AAGGTGAAGT AGAAAAACCA TTTATCAAGA CTGGATTTCC AGTGTCTGTA 
GAGAAAACTA CAAATAGTAA TCGCAAAAAT CAATTAGATA CCGGCCGAAG AAGACGCCAG 
TTTGATGAAG AGTCATTAGA AAGCTTTAGC AGTATGCCTG ATCCAGTAGA TCCGACAACA 
GTAACCAAAA CATTCAAGAC GAGAAAAGCA TCTGCACAGG CCAGCCTAGC ATCTAAAGAT 
AAAACTCCTA AATCAAAGAG TAAGAAAAGG CATTCTGCTC AACTAAAAAG CAGAGTTAAA 
AATACTGAAA CTGGGAGTGA TTTTTCCATG TTTGAAGCTT TGCGGGATAC TATTTATTCT 
GAAGTAGCTA CATTAATTTC TCAAAATGAA TCTCATCCAC ATTTTCTTAT TGAACTCTTC 
CATGAACTTC AGCTACTTAA TACAGACTAC TTGAGACAAA GGGCTTTATA TGCATTGCAG 
GACATAGTAA CCAGACATAT TTCTGAGAAC CATGAAAAAG AAGGAGAAAA TGTAAAGTCA 
GTGAATTCTG GTACTTGGAT AGCATCAAAC TCAGAGCTTA CCCCCAGTGA AAGCCTTGCT 
ACCACTGATG ATGAAACTTT TGAGAAGAAC TTTGAGAGAG AAACACATAA AATAAGTGAG 
CAAAATGATG CTGATAATGC TAGCGTCATG TCTGTATCTT CAAATTTTGA GCCTTTTGCA 
ACAGATGATC TAGGTAACAC TGTGATTCAC TTAGATCAGG CATTAGCCAG AATGAGGGAA 
TACGAGCGTA TGAAGACTGA GGCTGAAAGT AGCACAAATA TAAGATGCAC CTGCAGAATT 
CTTGAGGATG AGGATGGTGC TGCCGCCACA AGTATGGTTA CTAATTTAGA AGAAACTCCT 
ATTGAAAATC ATGGTTCACA ACAACCTGTA AGTGAAGTTT CTACTGTTCC ATGTCCTAGA 
ATTGATACTC AGCAGCTGGA CCGGCAAATT AAAGCCATTA TGAAAGAAGT CATTCCTTTT 
TTGAAGGAGC ACATGGATGA AGTATGCTCT TCTCAACTTC TAACTTCAGT AAGGCGTATG 
GTTTTGACCC TCACCCAACA AAATGATGAG AGCAAAGAAT TTGTGAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TTAATGAATT GGCTTTCTTT 
AAGCTCATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAAGTG CAAAAGGAAA 
ATAGAAGCAG CTGGAGTGAT ACAGTCTTAT GCCAAAGAGG CCAAAAGGAT TCTTGAAGGA 
GATCATGGCT CACCTGCTGG AGAAATTGAT GATGAAGACA AAGACAAGGA TGAAACTGAA 
ACAGTTAAGC CGACTCAGAC ATCTGAGATA TATGATGGTG ATGGTCCCAA AAATGTGAGA 
TCAGATGTTT CTGATCAAGA GGAAGATGAA GAAAGTGAAG AATGTCCGGT GTCTATTAAC 
TTGTCCAAAG CTGAAACTCA GGCTTTGACT AATTATGGAA GTGGAGAAGA TGAGAATGAG 
GATGAAGAAA TAGAAGAATT TGAAGAGGGA CCTGTGGATG TCCAGACGTC ACTTCAGGCT 
AACACTGAAG CTACAGAAGA AACTGAACAC GATGATCAGG TACTACAACA TGACTTTGAA 
AAATCAGGAG AAAGCAAAAA TGTCCCATCA GAACAAGATC CCACTACTAG TAAAGGCAAG 
AAATATGACC AAGATAGTAC TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAAATGAG 
CAGCCTTTGA ATAGCGCTGT CCAAAAAGAC TCTCTGACTA CCATCGATTC ATCTAAACAG 
CCTAACCCTT TGCCTTTACC TTTACCTGAA ATCGAAACCT TGGTGCCTAC AGTCAAAGAA 
GTTAAATCTG CTCAAGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA 
GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATACTC AGAGGCAGAT 
CTAAGGAAGA AAATGGTAGA AGAGCAAGAG AAAAACCATT TATCTGGTGA AATACTATGT 
GAAATGCAGA CCGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAAACAGTG 
GGAGCCCAAA GCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975621.1
CDS	615..6572
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_014120146.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120146.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGCAGATTGT CACTATAATA GAGAAGGAGA ACAGGGGATT 
CATGGTGCAC AAGGTGAAGA CGATGAGGAA GAAGAAGAAG CAGAAGATGA AGGAGTCAGT 
GGGGCTTCAT TAACTAGTCA TAGGAGCAGT CTGGTTGATG AGGCTGCAGA AGACGCTGAA 
TTTGAACAGA AGATCAATAG ACTTATGGCT GCCAAACAGA AATTAAGACA GTTACAAGAT 
CTTGTTGCTA TGGTACAGGA TGATGATGCA GCAGATCATG GAGTTATCTC TGCCAATACT 
TCAAATTTGG ATGATTTTTA CCCAGCAGAA GAAGACAACA AACAAAGTGC AAATAATACT 
AGAGGAAATG CCAATAAAAC ACAGAAAGAT GCTGGAATAA ATGAGAAGGC AAGAGAGAAG 
TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAGCTCA GACAATTGCA GGAAGAAAGA 
AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGCATTAC AGAAGGCATG CCCTGACTTA 
CAGCTGTCAG CCACTAGTGC GGGTAATTGT CCCACAAAAA AATATATCCC AGCTGTTACT 
TCAACCCCAG TTGTTAATGG GAATGAAACC AGTACAAGCA AATCTGCTTT TGAGCCTGCA 
GATCCTTCAG GAGTGGATAA TGAGCTGTGG TCAGAAATGC GAAGACATGA GATGTTAAGG 
GAAGAGCTAC GGCAGAGAAG AAAGCAGCTT GAAGCACTGA TGGCTGAACA TCAGAGGAGG 
CAAGGTCTAG CTGAAACTAC ATCCCCTCTG GCTGTGTCAT TGAGAAGTGA TGGATCTGAG 
AACCTGTGTA CGCCTCAACA GAGTAGAACA GAAAAAACCA TGGCAACATG GGGAGGTTCT 
ACCCAGTGTG CACTAGATGA AGAAGGAGAT GAAGATGGTT ACCTTTCTGA AGGAGTTGTT 
CGGACAGATG AAGAGGAGGA AGAAGAAGAG CAAGATGCAA GTTCGAATGA TAACTTTTCT 
ATGTATCCTC CTAACAGTGC GAATCATAAC TCTTACAACA TAAAAGAAAC TAAGAATAGG 
TGGAAGAATA GTCGCCCTTT TACAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA 
CAGCAGAATA TCAGCATGCA ACGTCAGGAA AACCTTCGTT GGATGTCCGA ACTCTCTTAC 
GTAGAGGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TGAAGAAACA ACTTGATTTC 
AGTGTCAATA TTTGTCAGAC TTTGATGCAA GACCAGCAGA CGCTGTCTTG TCTGCTGCAA 
ACTCTTCTCA CAGGTCCTTA CAGTGTTATG CCCAGCAATG TAGCTTCTCC TCAAGTACAC 
CTTATAATGC ACCAGTTGAA TCAGTGCTAT ACTCAGCTAA CATGGCAACA GAATAATGTG 
CAAAGGTTGA AACAAATGCT AAATGAACTT ATGCGCCAAC AGAATCAGCA CCCAGAAAAA 
CCTGGAAGCC AGGAAAGAGG CAGTAGTGCA CCACAGCCTT CTTCTCCCAG TTTATTTTGT 
CCTTTCAGCT TTCCATCACA GCCTGTAAAT CTATTTAACC TACCCGGGTT TACTAATTTT 
TCATCATTTG CACCAGGTAT GAATTTTAGC CCTTTATTTC CTTCTAATTT TGGAGAATTT 
TCTCAGAATA TTTCTACACC TACTGAACAG CAGCAACCAT TAGCCCAGAA TTCTTCAGGG 
AAAACAGAAT ATATGGCTTT TCCAAAACCT TTTGAAAGCA GTTCTTCTAT TGGAGCAGAA 
AAACAAAGGA ATCAAAAACA GCCTGGAGAG GAGGTGGAAA ACAGTAGGAC AGCGTGGTTA 
TATGACCAAG AAGGTGAAGT AGAAAAACCA TTTATCAAGA CTGGATTTCC AGTGTCTGTA 
GAGAAAACTA CAAATAGTAA TCGCAAAAAT CAATTAGATA CCGGCCGAAG AAGACGCCAG 
TTTGATGAAG AGTCATTAGA AAGCTTTAGC AGTATGCCTG ATCCAGTAGA TCCGACAACA 
GTAACCAAAA CATTCAAGAC GAGAAAAGCA TCTGCACAGG CCAGCCTAGC ATCTAAAGAT 
AAAACTCCTA AATCAAAGAG TAAGAAAAGG CATTCTGCTC AACTAAAAAG CAGAGTTAAA 
AATACTGAAA CTGGGAGTGA TTTTTCCATG TTTGAAGCTT TGCGGGATAC TATTTATTCT 
GAAGTAGCTA CATTAATTTC TCAAAATGAA TCTCATCCAC ATTTTCTTAT TGAACTCTTC 
CATGAACTTC AGCTACTTAA TACAGACTAC TTGAGACAAA GGGCTTTATA TGCATTGCAG 
GACATAGTAA CCAGACATAT TTCTGAGAAC CATGAAAAAG AAGGAGAAAA TGTAAAGTCA 
GTGAATTCTG GTACTTGGAT AGCATCAAAC TCAGAGCTTA CCCCCAGTGA AAGCCTTGCT 
ACCACTGATG ATGAAACTTT TGAGAAGAAC TTTGAGAGAG AAACACATAA AATAAGTGAG 
CAAAATGATG CTGATAATGC TAGCGTCATG TCTGTATCTT CAAATTTTGA GCCTTTTGCA 
ACAGATGATC TAGGTAACAC TGTGATTCAC TTAGATCAGG CATTAGCCAG AATGAGGGAA 
TACGAGCGTA TGAAGACTGA GGCTGAAAGT AGCACAAATA TAAGATGCAC CTGCAGAATT 
CTTGAGGATG AGGATGGTGC TGCCGCCACA AGTATGGTTA CTAATTTAGA AGAAACTCCT 
ATTGAAAATC ATGGTTCACA ACAACCTGTA AGTGAAGTTT CTACTGTTCC ATGTCCTAGA 
ATTGATACTC AGCAGCTGGA CCGGCAAATT AAAGCCATTA TGAAAGAAGT CATTCCTTTT 
TTGAAGGAGC ACATGGATGA AGTATGCTCT TCTCAACTTC TAACTTCAGT AAGGCGTATG 
GTTTTGACCC TCACCCAACA AAATGATGAG AGCAAAGAAT TTGTGAAGTT CTTTCATAAA 
CAACTTGGAA GTATATTACA GGATTCACTG GCAAAATTTG CTGGCAGAAA ACTGAAAGAC 
TGTGGAGAAG ATCTTCTTGT AGAGATATCT GAAGTGTTGT TTAATGAATT GGCTTTCTTT 
AAGCTCATGC AAGATTTGGA TAATAATAGT ATAACTGTTA AACAGAAGTG CAAAAGGAAA 
ATAGAAGCAG CTGGAGTGAT ACAGTCTTAT GCCAAAGAGG CCAAAAGGAT TCTTGAAGGA 
GATCATGGCT CACCTGCTGG AGAAATTGAT GATGAAGACA AAGACAAGGA TGAAACTGAA 
ACAGTTAAGC CGACTCAGAC ATCTGAGATA TATGATGGTG ATGGTCCCAA AAATGTGAGA 
TCAGATGTTT CTGATCAAGA GGAAGATGAA GAAAGTGAAG AATGTCCGGT GTCTATTAAC 
TTGTCCAAAG CTGAAACTCA GGCTTTGACT AATTATGGAA GTGGAGAAGA TGAGAATGAG 
GATGAAGAAA TAGAAGAATT TGAAGAGGGA CCTGTGGATG TCCAGACGTC ACTTCAGGCT 
AACACTGAAG CTACAGAAGA AACTGAACAC GATGATCAGG TACTACAACA TGACTTTGAA 
AAATCAGGAG AAAGCAAAAA TGTCCCATCA GAACAAGATC CCACTACTAG TAAAGGCAAG 
AAATATGACC AAGATAGTAC TCCTGTGAAA CCCTGTTACC TCAATATCTT GGAAAATGAG 
CAGCCTTTGA ATAGCGCTGT CCAAAAAGAC TCTCTGACTA CCATCGATTC ATCTAAACAG 
CCTAACCCTT TGCCTTTACC TTTACCTGAA ATCGAAACCT TGGTGCCTAC AGTCAAAGAA 
GTTAAATCTG CTCAAGAAAC TCCTGAAAGC TCTCTGGCTG GAAGTCCTGA TACTGAATCT 
CCAGTGTTAG TGAATGACTA TGAAGCAGAA TCTGGTAACA TAAGTCAAAA ATCTGATGAA 
GAAGACTTTG TGAAAGTTGA AGATTTACCA CTTAAACTGA CAATATACTC AGAGGCAGAT 
CTAAGGAAGA AAATGGTAGA AGAGCAAGAG AAAAACCATT TATCTGGTGA AATACTATGT 
GAAATGCAGA CCGAAGAATT AGCTGGGAAT TCTCAGACAC TAAAAGAACC TGAAACAGTG 
GGAGCCCAAA GCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404153.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259861.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X14, mRNA.

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CloneID	OCe294050
Clone ID Related Accession (Same CDS sequence)	XM_022404153.1
Accession Version	XM_022404153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGAT 
TGTCACTATA ATAGAGAAGG AGAACAGGGG ATTCATGGTG CACAAGGTGA AGACGATGAG 
GAAGAAGAAG AAGCAGAAGA TGAAGGAGTC AGTGGGGCTT CATTAACTAG TCATAGGAGC 
AGTCTGGTTG ATGAGGCTGC AGAAGACGCT GAATTTGAAC AGAAGATCAA TAGACTTATG 
GCTGCCAAAC AGAAATTAAG ACAGTTACAA GATCTTGTTG CTATGGTACA GGATGATGAT 
GCAGCAGATC ATGGAGTTAT CTCTGCCAAT ACTTCAAATT TGGATGATTT TTACCCAGCA 
GAAGAAGACA ACAAACAAAG TGCAAATAAT ACTAGAGGAA ATGCCAATAA AACACAGAAA 
GATGCTGGAA TAAATGAGAA GGCAAGAGAG AAGTTTTATG AGGCTAAACT ACAGCAGCAA 
CAGAGAGAGC TCAGACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA 
ATTCAAGCAT TACAGAAGGC ATGCCCTGAC TTACAGCTGT CAGCCACTAG TGCGGGTAAT 
TGTCCCACAA AAAAATATAT CCCAGCTGTT ACTTCAACCC CAGTTGTTAA TGGGAATGAA 
ACCAGTACAA GCAAATCTGC TTTTGAGCCT GCAGATCCTT CAGGAGTGGA TAATGAGCTG 
TGGTCAGAAA TGCGAAGACA TGAGATGTTA AGGGAAGAGC TACGGCAGAG AAGAAAGCAG 
CTTGAAGCAC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TAGCTGAAAC TACATCCCCT 
CTGGCTGTGT CATTGAGAAG TGATGGATCT GAGAACCTGT GTACGCCTCA ACAGAGTAGA 
ACAGAAAAAA CCATGGCAAC ATGGGGAGGT TCTACCCAGT GTGCACTAGA TGAAGAAGGA 
GATGAAGATG GTTACCTTTC TGAAGGAGTT GTTCGGACAG ATGAAGAGGA GGAAGAAGAA 
GAGCAAGATG CAAGTTCGAA TGATAACTTT TCTATGTATC CTCCTAACAG TGCGAATCAT 
AACTCTTACA ACATAAAAGA AACTAAGAAT AGGTGGAAGA ATAGTCGCCC TTTTACAGCA 
GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAGCAGA ATATCAGCAT GCAACGTCAG 
GAAAACCTTC GTTGGATGTC CGAACTCTCT TACGTAGAGG AGAAAGAACA ATGGCAAGAA 
CAAATTAATC AGCTGAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTGATG 
CAAGACCAGC AGACGCTGTC TTGTCTGCTG CAAACTCTTC TCACAGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTAGCTTC TCCTCAAGTA CACCTTATAA TGCACCAGTT GAATCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTGCAAAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AACAGAATCA GCACCCAGAA AAACCTGGAA GCCAGGAAAG AGGCAGTAGT 
GCACCACAGC CTTCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCATC ACAGCCTGTA 
AATCTATTTA ACCTACCCGG GTTTACTAAT TTTTCATCAT TTGCACCAGG TATGAATTTT 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAA TTTTCTCAGA ATATTTCTAC ACCTACTGAA 
CAGCAGCAAC CATTAGCCCA GAATTCTTCA GGGAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGGA 
GAGGAGGTGG AAAACAGTAG GACAGCGTGG TTATATGACC AAGAAGGTGA AGTAGAAAAA 
CCATTTATCA AGACTGGATT TCCAGTGTCT GTAGAGAAAA CTACAAATAG TAATCGCAAA 
AATCAATTAG ATACCGGCCG AAGAAGACGC CAGTTTGATG AAGAGTCATT AGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCGACA ACAGTAACCA AAACATTCAA GACGAGAAAA 
GCATCTGCAC AGGCCAGCCT AGCATCTAAA GATAAAACTC CTAAATCAAA GAGTAAGAAA 
AGGCATTCTG CTCAACTAAA AAGCAGAGTT AAAAATACTG AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGGGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCATC CACATTTTCT TATTGAACTC TTCCATGAAC TTCAGCTACT TAATACAGAC 
TACTTGAGAC AAAGGGCTTT ATATGCATTG CAGGACATAG TAACCAGACA TATTTCTGAG 
AACCATGAAA AAGAAGGAGA AAATGTAAAG TCAGTGAATT CTGGTACTTG GATAGCATCA 
AACTCAGAGC TTACCCCCAG TGAAAGCCTT GCTACCACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAGA GAGAAACACA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGCGTC 
ATGTCTGTAT CTTCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACTGTGATT 
CACTTAGATC AGGCATTAGC CAGAATGAGG GAATACGAGC GTATGAAGAC TGAGGCTGAA 
AGTAGCACAA ATATAAGATG CACCTGCAGA ATTCTTGAGG ATGAGGATGG TGCTGCCGCC 
ACAAGTATGG TTACTAATTT AGAAGAAACT CCTATTGAAA ATCATGGTTC ACAACAACCT 
GTAAGTGAAG TTTCTACTGT TCCATGTCCT AGAATTGATA CTCAGCAGCT GGACCGGCAA 
ATTAAAGCCA TTATGAAAGA AGTCATTCCT TTTTTGAAGG AGCACATGGA TGAAGTATGC 
TCTTCTCAAC TTCTAACTTC AGTAAGGCGT ATGGTTTTGA CCCTCACCCA ACAAAATGAT 
GAGAGCAAAG AATTTGTGAA GTTCTTTCAT AAACAACTTG GAAGTATATT ACAGGATTCA 
CTGGCAAAAT TTGCTGGCAG AAAACTGAAA GACTGTGGAG AAGATCTTCT TGTAGAGATA 
TCTGAAGTGT TGTTTAATGA ATTGGCTTTC TTTAAGCTCA TGCAAGATTT GGATAATAAT 
AGTATAACTG TTAAACAGAA GTGCAAAAGG AAAATAGAAG CAGCTGGAGT GATACAGTCT 
TATGCCAAAG AGGCCAAAAG GATTCTTGAA GGAGATCATG GCTCACCTGC TGGAGAAATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCCGACTCA GACATCTGAG 
ATATATGATG GTGATGGTCC CAAAAATGTG AGATCAGATG TTTCTGATCA AGAGGAAGAT 
GAAGAAAGTG AAGAATGTCC GGTGTCTATT AACTTGTCCA AAGCTGAAAC TCAGGCTTTG 
ACTAATTATG GAAGTGGAGA AGATGAGAAT GAGGATGAAG AAATAGAAGA ATTTGAAGAG 
GGACCTGTGG ATGTCCAGAC GTCACTTCAG GCTAACACTG AAGCTACAGA AGAAACTGAA 
CACGATGATC AGGTACTACA ACATGACTTT GAAAAATCAG GAGAAAGCAA AAATGTCCCA 
TCAGAACAAG ATCCCACTAC TAGTAAAGGC AAGAAATATG ACCAAGATAG TACTCCTGTG 
AAACCCTGTT ACCTCAATAT CTTGGAAAAT GAGCAGCCTT TGAATAGCGC TGTCCAAAAA 
GACTCTCTGA CTACCATCGA TTCATCTAAA CAGCCTAACC CTTTGCCTTT ACCTTTACCT 
GAAATCGAAA CCTTGGTGCC TACAGTCAAA GAAGTTAAAT CTGCTCAAGA AACTCCTGAA 
AGCTCTCTGG CTGGAAGTCC TGATACTGAA TCTCCAGTGT TAGTGAATGA CTATGAAGCA 
GAATCTGGTA ACATAAGTCA AAAATCTGAT GAAGAAGACT TTGTGAAAGT TGAAGATTTA 
CCACTTAAAC TGACAATATA CTCAGAGGCA GATCTAAGGA AGAAAATGGT AGAAGAGCAA 
GAGAAAAACC ATTTATCTGG TGAAATACTA TGTGAAATGC AGACCGAAGA ATTAGCTGGG 
AATTCTCAGA CACTAAAAGA ACCTGAAACA GTGGGAGCCC AAAGCGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259861.1
CDS	426..6776
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLDCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404153.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404153.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGAT 
TGTCACTATA ATAGAGAAGG AGAACAGGGG ATTCATGGTG CACAAGGTGA AGACGATGAG 
GAAGAAGAAG AAGCAGAAGA TGAAGGAGTC AGTGGGGCTT CATTAACTAG TCATAGGAGC 
AGTCTGGTTG ATGAGGCTGC AGAAGACGCT GAATTTGAAC AGAAGATCAA TAGACTTATG 
GCTGCCAAAC AGAAATTAAG ACAGTTACAA GATCTTGTTG CTATGGTACA GGATGATGAT 
GCAGCAGATC ATGGAGTTAT CTCTGCCAAT ACTTCAAATT TGGATGATTT TTACCCAGCA 
GAAGAAGACA ACAAACAAAG TGCAAATAAT ACTAGAGGAA ATGCCAATAA AACACAGAAA 
GATGCTGGAA TAAATGAGAA GGCAAGAGAG AAGTTTTATG AGGCTAAACT ACAGCAGCAA 
CAGAGAGAGC TCAGACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA 
ATTCAAGCAT TACAGAAGGC ATGCCCTGAC TTACAGCTGT CAGCCACTAG TGCGGGTAAT 
TGTCCCACAA AAAAATATAT CCCAGCTGTT ACTTCAACCC CAGTTGTTAA TGGGAATGAA 
ACCAGTACAA GCAAATCTGC TTTTGAGCCT GCAGATCCTT CAGGAGTGGA TAATGAGCTG 
TGGTCAGAAA TGCGAAGACA TGAGATGTTA AGGGAAGAGC TACGGCAGAG AAGAAAGCAG 
CTTGAAGCAC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TAGCTGAAAC TACATCCCCT 
CTGGCTGTGT CATTGAGAAG TGATGGATCT GAGAACCTGT GTACGCCTCA ACAGAGTAGA 
ACAGAAAAAA CCATGGCAAC ATGGGGAGGT TCTACCCAGT GTGCACTAGA TGAAGAAGGA 
GATGAAGATG GTTACCTTTC TGAAGGAGTT GTTCGGACAG ATGAAGAGGA GGAAGAAGAA 
GAGCAAGATG CAAGTTCGAA TGATAACTTT TCTATGTATC CTCCTAACAG TGCGAATCAT 
AACTCTTACA ACATAAAAGA AACTAAGAAT AGGTGGAAGA ATAGTCGCCC TTTTACAGCA 
GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAGCAGA ATATCAGCAT GCAACGTCAG 
GAAAACCTTC GTTGGATGTC CGAACTCTCT TACGTAGAGG AGAAAGAACA ATGGCAAGAA 
CAAATTAATC AGCTGAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTGATG 
CAAGACCAGC AGACGCTGTC TTGTCTGCTG CAAACTCTTC TCACAGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTAGCTTC TCCTCAAGTA CACCTTATAA TGCACCAGTT GAATCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTGCAAAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AACAGAATCA GCACCCAGAA AAACCTGGAA GCCAGGAAAG AGGCAGTAGT 
GCACCACAGC CTTCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCATC ACAGCCTGTA 
AATCTATTTA ACCTACCCGG GTTTACTAAT TTTTCATCAT TTGCACCAGG TATGAATTTT 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAA TTTTCTCAGA ATATTTCTAC ACCTACTGAA 
CAGCAGCAAC CATTAGCCCA GAATTCTTCA GGGAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGGA 
GAGGAGGTGG AAAACAGTAG GACAGCGTGG TTATATGACC AAGAAGGTGA AGTAGAAAAA 
CCATTTATCA AGACTGGATT TCCAGTGTCT GTAGAGAAAA CTACAAATAG TAATCGCAAA 
AATCAATTAG ATACCGGCCG AAGAAGACGC CAGTTTGATG AAGAGTCATT AGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCGACA ACAGTAACCA AAACATTCAA GACGAGAAAA 
GCATCTGCAC AGGCCAGCCT AGCATCTAAA GATAAAACTC CTAAATCAAA GAGTAAGAAA 
AGGCATTCTG CTCAACTAAA AAGCAGAGTT AAAAATACTG AAACTGGGAG TGATTTTTCC 
ATGTTTGAAG CTTTGCGGGA TACTATTTAT TCTGAAGTAG CTACATTAAT TTCTCAAAAT 
GAATCTCATC CACATTTTCT TATTGAACTC TTCCATGAAC TTCAGCTACT TAATACAGAC 
TACTTGAGAC AAAGGGCTTT ATATGCATTG CAGGACATAG TAACCAGACA TATTTCTGAG 
AACCATGAAA AAGAAGGAGA AAATGTAAAG TCAGTGAATT CTGGTACTTG GATAGCATCA 
AACTCAGAGC TTACCCCCAG TGAAAGCCTT GCTACCACTG ATGATGAAAC TTTTGAGAAG 
AACTTTGAGA GAGAAACACA TAAAATAAGT GAGCAAAATG ATGCTGATAA TGCTAGCGTC 
ATGTCTGTAT CTTCAAATTT TGAGCCTTTT GCAACAGATG ATCTAGGTAA CACTGTGATT 
CACTTAGATC AGGCATTAGC CAGAATGAGG GAATACGAGC GTATGAAGAC TGAGGCTGAA 
AGTAGCACAA ATATAAGATG CACCTGCAGA ATTCTTGAGG ATGAGGATGG TGCTGCCGCC 
ACAAGTATGG TTACTAATTT AGAAGAAACT CCTATTGAAA ATCATGGTTC ACAACAACCT 
GTAAGTGAAG TTTCTACTGT TCCATGTCCT AGAATTGATA CTCAGCAGCT GGACCGGCAA 
ATTAAAGCCA TTATGAAAGA AGTCATTCCT TTTTTGAAGG AGCACATGGA TGAAGTATGC 
TCTTCTCAAC TTCTAACTTC AGTAAGGCGT ATGGTTTTGA CCCTCACCCA ACAAAATGAT 
GAGAGCAAAG AATTTGTGAA GTTCTTTCAT AAACAACTTG GAAGTATATT ACAGGATTCA 
CTGGCAAAAT TTGCTGGCAG AAAACTGAAA GACTGTGGAG AAGATCTTCT TGTAGAGATA 
TCTGAAGTGT TGTTTAATGA ATTGGCTTTC TTTAAGCTCA TGCAAGATTT GGATAATAAT 
AGTATAACTG TTAAACAGAA GTGCAAAAGG AAAATAGAAG CAGCTGGAGT GATACAGTCT 
TATGCCAAAG AGGCCAAAAG GATTCTTGAA GGAGATCATG GCTCACCTGC TGGAGAAATT 
GATGATGAAG ACAAAGACAA GGATGAAACT GAAACAGTTA AGCCGACTCA GACATCTGAG 
ATATATGATG GTGATGGTCC CAAAAATGTG AGATCAGATG TTTCTGATCA AGAGGAAGAT 
GAAGAAAGTG AAGAATGTCC GGTGTCTATT AACTTGTCCA AAGCTGAAAC TCAGGCTTTG 
ACTAATTATG GAAGTGGAGA AGATGAGAAT GAGGATGAAG AAATAGAAGA ATTTGAAGAG 
GGACCTGTGG ATGTCCAGAC GTCACTTCAG GCTAACACTG AAGCTACAGA AGAAACTGAA 
CACGATGATC AGGTACTACA ACATGACTTT GAAAAATCAG GAGAAAGCAA AAATGTCCCA 
TCAGAACAAG ATCCCACTAC TAGTAAAGGC AAGAAATATG ACCAAGATAG TACTCCTGTG 
AAACCCTGTT ACCTCAATAT CTTGGAAAAT GAGCAGCCTT TGAATAGCGC TGTCCAAAAA 
GACTCTCTGA CTACCATCGA TTCATCTAAA CAGCCTAACC CTTTGCCTTT ACCTTTACCT 
GAAATCGAAA CCTTGGTGCC TACAGTCAAA GAAGTTAAAT CTGCTCAAGA AACTCCTGAA 
AGCTCTCTGG CTGGAAGTCC TGATACTGAA TCTCCAGTGT TAGTGAATGA CTATGAAGCA 
GAATCTGGTA ACATAAGTCA AAAATCTGAT GAAGAAGACT TTGTGAAAGT TGAAGATTTA 
CCACTTAAAC TGACAATATA CTCAGAGGCA GATCTAAGGA AGAAAATGGT AGAAGAGCAA 
GAGAAAAACC ATTTATCTGG TGAAATACTA TGTGAAATGC AGACCGAAGA ATTAGCTGGG 
AATTCTCAGA CACTAAAAGA ACCTGAAACA GTGGGAGCCC AAAGCGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404154.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259862.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X15, mRNA.

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CloneID	OCe294051
Clone ID Related Accession (Same CDS sequence)	XM_022404154.1
Accession Version	XM_022404154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAGCATGAC 
TTATTAAAAA GGATGTTACA ACAGCAGGAG CAACTGAGAG CTCTGCAGGG AAGACAGGCT 
GCTCTTCTTG CTCTGCAGCA CAAAGCAGAG CAAGCTATTG CTGTGATGGA TGATTCTGTT 
GTAACAGAAA CTACAGGTAG CGTATCTGGA GTTAGTATCA CCTCTGAACT AAATGAAGAA 
TTGAATGATT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAACTGTT 
CCAGACAATA GAAGACAAGC AGAAAGTCTT TCATTAACTA GGGAGGTATC CCAGAGCAGG 
AATCCATCAG TTTCAGAACA CTTACCTGAT GAGAAAGTAC AACTGTTTAG CAAAATGAGA 
GTATTACAGG AAAAGAAACA AAAAATGGAC AAACTACTTG GAGAACTTCA TACACTTCGA 
GATCAACATC TGAACAATTC ATCATTTGTG CCTTCTTCAG CTTCTCCACA AAGAAGTGTG 
GATCAGAGAA GTACAACTTC AGCACCCTCT GCTCCTATAG GCTTAGCACC AGTTGTCAAT 
GGAGAATCCA ACAGCTTCAC ATCATCTGTT CCTTATCCTG TTGCTTCTCT AGTTTCTCAG 
AATGAGAGTG AAAACGAAGG ACATCTAAAT CCAACTGAAA AACTCCAGAA GTTAAATGAA 
GTTCGAAAGA GATTGAATGA ACTAAGAGAA CTAGTTCATT ATTACGAACA AACATCAGAT 
ATGATGACAG ATGCTGTAAA TGAAAACACA AAAGATGAAG AAACTGAAGA GTCAGAATAT 
GATTCTGAAC ATGAAAACCC TGAACCTGTT ACTAACATTC GAAATCCTCA AGTAGCTGCC 
ACTTGGAATG AAGTAAATAG TAATTCTAAT GCACAGTGTG TTTCTAATAA TAGAGAGGGG 
AGATCAGTTA ATTCTAATTG TGAAATTAAC AACAGATCTG CTGCCAACAT AAGGACTCTG 
AACATGCCTC CTTCTTTAGC AGATTGTCAC TATAATAGAG AAGGAGAACA GGGGATTCAT 
GGTGCACAAG GTGAAGACGA TGAGGAAGAA GAAGAAGCAG AAGATGAAGG AGTCAGTGGG 
GCTTCATTAA CTAGTCATAG GAGCAGTCTG GTTGATGAGG CTGCAGAAGA CGCTGAATTT 
GAACAGAAGA TCAATAGACT TATGGCTGCC AAACAGAAAT TAAGACAGTT ACAAGATCTT 
GTTGCTATGG TACAGGATGA TGATGCAGCA GATCATGGAG TTATCTCTGC CAATACTTCA 
AATTTGGATG ATTTTTACCC AGCAGAAGAA GACAACAAAC AAAGTGCAAA TAATACTAGA 
GGAAATGCCA ATAAAACACA GAAAGATGCT GGAATAAATG AGAAGGCAAG AGAGAAGTTT 
TATGAGGCTA AACTACAGCA GCAACAGAGA GAGCTCAGAC AATTGCAGGA AGAAAGAAAG 
AAACTGATTG AGATTCAAGA GAAAATTCAA GCATTACAGA AGGCATGCCC TGACTTACAG 
CTGTCAGCCA CTAGTGCGGG TAATTGTCCC ACAAAAAAAT ATATCCCAGC TGTTACTTCA 
ACCCCAGTTG TTAATGGGAA TGAAACCAGT ACAAGCAAAT CTGCTTTTGA GCCTGCAGAT 
CCTTCAGGAG TGGATAATGA GCTGTGGTCA GAAATGCGAA GACATGAGAT GTTAAGGGAA 
GAGCTACGGC AGAGAAGAAA GCAGCTTGAA GCACTGATGG CTGAACATCA GAGGAGGCAA 
GGTCTAGCTG AAACTACATC CCCTCTGGCT GTGTCATTGA GAAGTGATGG ATCTGAGAAC 
CTGTGTACGC CTCAACAGAG TAGAACAGAA AAAACCATGG CAACATGGGG AGGTTCTACC 
CAGTGTGCAC TAGATGAAGA AGGAGATGAA GATGGTTACC TTTCTGAAGG AGTTGTTCGG 
ACAGATGAAG AGGAGGAAGA AGAAGAGCAA GATGCAAGTT CGAATGATAA CTTTTCTATG 
TATCCTCCTA ACAGTGCGAA TCATAACTCT TACAACATAA AAGAAACTAA GAATAGGTGG 
AAGAATAGTC GCCCTTTTAC AGCAGATGGA AATTATCGTC CTTTAGCCAA GACAAGACAG 
CAGAATATCA GCATGCAACG TCAGGAAAAC CTTCGTTGGA TGTCCGAACT CTCTTACGTA 
GAGGAGAAAG AACAATGGCA AGAACAAATT AATCAGCTGA AGAAACAACT TGATTTCAGT 
GTCAATATTT GTCAGACTTT GATGCAAGAC CAGCAGACGC TGTCTTGTCT GCTGCAAACT 
CTTCTCACAG GTCCTTACAG TGTTATGCCC AGCAATGTAG CTTCTCCTCA AGTACACCTT 
ATAATGCACC AGTTGAATCA GTGCTATACT CAGCTAACAT GGCAACAGAA TAATGTGCAA 
AGGTTGAAAC AAATGCTAAA TGAACTTATG CGCCAACAGA ATCAGCACCC AGAAAAACCT 
GGAAGCCAGG AAAGAGGCAG TAGTGCACCA CAGCCTTCTT CTCCCAGTTT ATTTTGTCCT 
TTCAGCTTTC CATCACAGCC TGTAAATCTA TTTAACCTAC CCGGGTTTAC TAATTTTTCA 
TCATTTGCAC CAGGTATGAA TTTTAGCCCT TTATTTCCTT CTAATTTTGG AGAATTTTCT 
CAGAATATTT CTACACCTAC TGAACAGCAG CAACCATTAG CCCAGAATTC TTCAGGGAAA 
ACAGAATATA TGGCTTTTCC AAAACCTTTT GAAAGCAGTT CTTCTATTGG AGCAGAAAAA 
CAAAGGAATC AAAAACAGCC TGGAGAGGAG GTGGAAAACA GTAGGACAGC GTGGTTATAT 
GACCAAGAAG GTGAAGTAGA AAAACCATTT ATCAAGACTG GATTTCCAGT GTCTGTAGAG 
AAAACTACAA ATAGTAATCG CAAAAATCAA TTAGATACCG GCCGAAGAAG ACGCCAGTTT 
GATGAAGAGT CATTAGAAAG CTTTAGCAGT ATGCCTGATC CAGTAGATCC GACAACAGTA 
ACCAAAACAT TCAAGACGAG AAAAGCATCT GCACAGGCCA GCCTAGCATC TAAAGATAAA 
ACTCCTAAAT CAAAGAGTAA GAAAAGGCAT TCTGCTCAAC TAAAAAGCAG AGTTAAAAAT 
ACTGGGTATG AAAGTGCCAG TGTGTCTAGC ACATGTGAAC CTTGCAAAAG TAGGAACAGA 
CATTCAGCCC AGACTGAAGA GCCTGTTCAA GCAAAAGTAT TCAGCAGAAA GAATCTTGAG 
CAGCTGGAAA AAATAATAAA ATATAGTAGG TCTACAGAGA TATCTTCAGC ACATGCTAGG 
AGAATACTAC AGCAGTCTAA CAGAAATGCA TGCAATGAAG CACCAGAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGGGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCATCCACA TTTTCTTATT GAACTCTTCC ATGAACTTCA GCTACTTAAT 
ACAGACTACT TGAGACAAAG GGCTTTATAT GCATTGCAGG ACATAGTAAC CAGACATATT 
TCTGAGAACC ATGAAAAAGA AGGAGAAAAT GTAAAGTCAG TGAATTCTGG TACTTGGATA 
GCATCAAACT CAGAGCTTAC CCCCAGTGAA AGCCTTGCTA CCACTGATGA TGAAACTTTT 
GAGAAGAACT TTGAGAGAGA AACACATAAA ATAAGTGAGC AAAATGATGC TGATAATGCT 
AGCGTCATGT CTGTATCTTC AAATTTTGAG CCTTTTGCAA CAGATGATCT AGGTAACACT 
GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGGGAAT ACGAGCGTAT GAAGACTGAG 
GCTGAAAGTA GCACAAATAT AAGATGCACC TGCAGAATTC TTGAGGATGA GGATGGTGCT 
GCCGCCACAA GTATGGTTAC TAATTTAGAA GAAACTCCTA TTGAAAATCA TGGTTCACAA 
CAACCTGTAA GTGAAGTTTC TACTGTTCCA TGTCCTAGAA TTGATACTCA GCAGCTGGAC 
CGGCAAATTA AAGCCATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGAGCA CATGGATGAA 
GTATGCTCTT CTCAACTTCT AACTTCAGTA AGGCGTATGG TTTTGACCCT CACCCAACAA 
AATGATGAGA GCAAAGAATT TGTGAAGTTC TTTCATAAAC AACTTGGAAG TATATTACAG 
GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAAGACT GTGGAGAAGA TCTTCTTGTA 
GAGATATCTG AAGTGTTGTT TAATGAATTG GCTTTCTTTA AGCTCATGCA AGATTTGGAT 
AATAATAGTA TAACTGTTAA ACAGAAGTGC AAAAGGAAAA TAGAAGCAGC TGGAGTGATA 
CAGTCTTATG CCAAAGAGGC CAAAAGGATT CTTGAAGGAG ATCATGGCTC ACCTGCTGGA 
GAAATTGATG ATGAAGACAA AGACAAGGAT GAAACTGAAA CAGTTAAGCC GACTCAGACA 
TCTGAGATAT ATGATGGTGA TGGTCCCAAA AATGTGAGAT CAGATGTTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGA ATGTCCGGTG TCTATTAACT TGTCCAAAGC TGAAACTCAG 
GCTTTGACTA ATTATGGAAG TGGAGAAGAT GAGAATGAGG ATGAAGAAAT AGAAGAATTT 
GAAGAGGGAC CTGTGGATGT CCAGACGTCA CTTCAGGCTA ACACTGAAGC TACAGAAGAA 
ACTGAACACG ATGATCAGGT ACTACAACAT GACTTTGAAA AATCAGGAGA AAGCAAAAAT 
GTCCCATCAG AACAAGATCC CACTACTAGT AAAGGCAAGA AATATGACCA AGATAGTACT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAAATGAGC AGCCTTTGAA TAGCGCTGTC 
CAAAAAGACT CTCTGACTAC CATCGATTCA TCTAAACAGC CTAACCCTTT GCCTTTACCT 
TTACCTGAAA TCGAAACCTT GGTGCCTACA GTCAAAGAAG TTAAATCTGC TCAAGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA 
GATTTACCAC TTAAACTGAC AATATACTCA GAGGCAGATC TAAGGAAGAA AATGGTAGAA 
GAGCAAGAGA AAAACCATTT ATCTGGTGAA ATACTATGTG AAATGCAGAC CGAAGAATTA 
GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259862.1
CDS	426..6722
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404154.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404154.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAGCATGAC 
TTATTAAAAA GGATGTTACA ACAGCAGGAG CAACTGAGAG CTCTGCAGGG AAGACAGGCT 
GCTCTTCTTG CTCTGCAGCA CAAAGCAGAG CAAGCTATTG CTGTGATGGA TGATTCTGTT 
GTAACAGAAA CTACAGGTAG CGTATCTGGA GTTAGTATCA CCTCTGAACT AAATGAAGAA 
TTGAATGATT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAACTGTT 
CCAGACAATA GAAGACAAGC AGAAAGTCTT TCATTAACTA GGGAGGTATC CCAGAGCAGG 
AATCCATCAG TTTCAGAACA CTTACCTGAT GAGAAAGTAC AACTGTTTAG CAAAATGAGA 
GTATTACAGG AAAAGAAACA AAAAATGGAC AAACTACTTG GAGAACTTCA TACACTTCGA 
GATCAACATC TGAACAATTC ATCATTTGTG CCTTCTTCAG CTTCTCCACA AAGAAGTGTG 
GATCAGAGAA GTACAACTTC AGCACCCTCT GCTCCTATAG GCTTAGCACC AGTTGTCAAT 
GGAGAATCCA ACAGCTTCAC ATCATCTGTT CCTTATCCTG TTGCTTCTCT AGTTTCTCAG 
AATGAGAGTG AAAACGAAGG ACATCTAAAT CCAACTGAAA AACTCCAGAA GTTAAATGAA 
GTTCGAAAGA GATTGAATGA ACTAAGAGAA CTAGTTCATT ATTACGAACA AACATCAGAT 
ATGATGACAG ATGCTGTAAA TGAAAACACA AAAGATGAAG AAACTGAAGA GTCAGAATAT 
GATTCTGAAC ATGAAAACCC TGAACCTGTT ACTAACATTC GAAATCCTCA AGTAGCTGCC 
ACTTGGAATG AAGTAAATAG TAATTCTAAT GCACAGTGTG TTTCTAATAA TAGAGAGGGG 
AGATCAGTTA ATTCTAATTG TGAAATTAAC AACAGATCTG CTGCCAACAT AAGGACTCTG 
AACATGCCTC CTTCTTTAGC AGATTGTCAC TATAATAGAG AAGGAGAACA GGGGATTCAT 
GGTGCACAAG GTGAAGACGA TGAGGAAGAA GAAGAAGCAG AAGATGAAGG AGTCAGTGGG 
GCTTCATTAA CTAGTCATAG GAGCAGTCTG GTTGATGAGG CTGCAGAAGA CGCTGAATTT 
GAACAGAAGA TCAATAGACT TATGGCTGCC AAACAGAAAT TAAGACAGTT ACAAGATCTT 
GTTGCTATGG TACAGGATGA TGATGCAGCA GATCATGGAG TTATCTCTGC CAATACTTCA 
AATTTGGATG ATTTTTACCC AGCAGAAGAA GACAACAAAC AAAGTGCAAA TAATACTAGA 
GGAAATGCCA ATAAAACACA GAAAGATGCT GGAATAAATG AGAAGGCAAG AGAGAAGTTT 
TATGAGGCTA AACTACAGCA GCAACAGAGA GAGCTCAGAC AATTGCAGGA AGAAAGAAAG 
AAACTGATTG AGATTCAAGA GAAAATTCAA GCATTACAGA AGGCATGCCC TGACTTACAG 
CTGTCAGCCA CTAGTGCGGG TAATTGTCCC ACAAAAAAAT ATATCCCAGC TGTTACTTCA 
ACCCCAGTTG TTAATGGGAA TGAAACCAGT ACAAGCAAAT CTGCTTTTGA GCCTGCAGAT 
CCTTCAGGAG TGGATAATGA GCTGTGGTCA GAAATGCGAA GACATGAGAT GTTAAGGGAA 
GAGCTACGGC AGAGAAGAAA GCAGCTTGAA GCACTGATGG CTGAACATCA GAGGAGGCAA 
GGTCTAGCTG AAACTACATC CCCTCTGGCT GTGTCATTGA GAAGTGATGG ATCTGAGAAC 
CTGTGTACGC CTCAACAGAG TAGAACAGAA AAAACCATGG CAACATGGGG AGGTTCTACC 
CAGTGTGCAC TAGATGAAGA AGGAGATGAA GATGGTTACC TTTCTGAAGG AGTTGTTCGG 
ACAGATGAAG AGGAGGAAGA AGAAGAGCAA GATGCAAGTT CGAATGATAA CTTTTCTATG 
TATCCTCCTA ACAGTGCGAA TCATAACTCT TACAACATAA AAGAAACTAA GAATAGGTGG 
AAGAATAGTC GCCCTTTTAC AGCAGATGGA AATTATCGTC CTTTAGCCAA GACAAGACAG 
CAGAATATCA GCATGCAACG TCAGGAAAAC CTTCGTTGGA TGTCCGAACT CTCTTACGTA 
GAGGAGAAAG AACAATGGCA AGAACAAATT AATCAGCTGA AGAAACAACT TGATTTCAGT 
GTCAATATTT GTCAGACTTT GATGCAAGAC CAGCAGACGC TGTCTTGTCT GCTGCAAACT 
CTTCTCACAG GTCCTTACAG TGTTATGCCC AGCAATGTAG CTTCTCCTCA AGTACACCTT 
ATAATGCACC AGTTGAATCA GTGCTATACT CAGCTAACAT GGCAACAGAA TAATGTGCAA 
AGGTTGAAAC AAATGCTAAA TGAACTTATG CGCCAACAGA ATCAGCACCC AGAAAAACCT 
GGAAGCCAGG AAAGAGGCAG TAGTGCACCA CAGCCTTCTT CTCCCAGTTT ATTTTGTCCT 
TTCAGCTTTC CATCACAGCC TGTAAATCTA TTTAACCTAC CCGGGTTTAC TAATTTTTCA 
TCATTTGCAC CAGGTATGAA TTTTAGCCCT TTATTTCCTT CTAATTTTGG AGAATTTTCT 
CAGAATATTT CTACACCTAC TGAACAGCAG CAACCATTAG CCCAGAATTC TTCAGGGAAA 
ACAGAATATA TGGCTTTTCC AAAACCTTTT GAAAGCAGTT CTTCTATTGG AGCAGAAAAA 
CAAAGGAATC AAAAACAGCC TGGAGAGGAG GTGGAAAACA GTAGGACAGC GTGGTTATAT 
GACCAAGAAG GTGAAGTAGA AAAACCATTT ATCAAGACTG GATTTCCAGT GTCTGTAGAG 
AAAACTACAA ATAGTAATCG CAAAAATCAA TTAGATACCG GCCGAAGAAG ACGCCAGTTT 
GATGAAGAGT CATTAGAAAG CTTTAGCAGT ATGCCTGATC CAGTAGATCC GACAACAGTA 
ACCAAAACAT TCAAGACGAG AAAAGCATCT GCACAGGCCA GCCTAGCATC TAAAGATAAA 
ACTCCTAAAT CAAAGAGTAA GAAAAGGCAT TCTGCTCAAC TAAAAAGCAG AGTTAAAAAT 
ACTGGGTATG AAAGTGCCAG TGTGTCTAGC ACATGTGAAC CTTGCAAAAG TAGGAACAGA 
CATTCAGCCC AGACTGAAGA GCCTGTTCAA GCAAAAGTAT TCAGCAGAAA GAATCTTGAG 
CAGCTGGAAA AAATAATAAA ATATAGTAGG TCTACAGAGA TATCTTCAGC ACATGCTAGG 
AGAATACTAC AGCAGTCTAA CAGAAATGCA TGCAATGAAG CACCAGAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGGGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCATCCACA TTTTCTTATT GAACTCTTCC ATGAACTTCA GCTACTTAAT 
ACAGACTACT TGAGACAAAG GGCTTTATAT GCATTGCAGG ACATAGTAAC CAGACATATT 
TCTGAGAACC ATGAAAAAGA AGGAGAAAAT GTAAAGTCAG TGAATTCTGG TACTTGGATA 
GCATCAAACT CAGAGCTTAC CCCCAGTGAA AGCCTTGCTA CCACTGATGA TGAAACTTTT 
GAGAAGAACT TTGAGAGAGA AACACATAAA ATAAGTGAGC AAAATGATGC TGATAATGCT 
AGCGTCATGT CTGTATCTTC AAATTTTGAG CCTTTTGCAA CAGATGATCT AGGTAACACT 
GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGGGAAT ACGAGCGTAT GAAGACTGAG 
GCTGAAAGTA GCACAAATAT AAGATGCACC TGCAGAATTC TTGAGGATGA GGATGGTGCT 
GCCGCCACAA GTATGGTTAC TAATTTAGAA GAAACTCCTA TTGAAAATCA TGGTTCACAA 
CAACCTGTAA GTGAAGTTTC TACTGTTCCA TGTCCTAGAA TTGATACTCA GCAGCTGGAC 
CGGCAAATTA AAGCCATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGAGCA CATGGATGAA 
GTATGCTCTT CTCAACTTCT AACTTCAGTA AGGCGTATGG TTTTGACCCT CACCCAACAA 
AATGATGAGA GCAAAGAATT TGTGAAGTTC TTTCATAAAC AACTTGGAAG TATATTACAG 
GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAAGACT GTGGAGAAGA TCTTCTTGTA 
GAGATATCTG AAGTGTTGTT TAATGAATTG GCTTTCTTTA AGCTCATGCA AGATTTGGAT 
AATAATAGTA TAACTGTTAA ACAGAAGTGC AAAAGGAAAA TAGAAGCAGC TGGAGTGATA 
CAGTCTTATG CCAAAGAGGC CAAAAGGATT CTTGAAGGAG ATCATGGCTC ACCTGCTGGA 
GAAATTGATG ATGAAGACAA AGACAAGGAT GAAACTGAAA CAGTTAAGCC GACTCAGACA 
TCTGAGATAT ATGATGGTGA TGGTCCCAAA AATGTGAGAT CAGATGTTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGA ATGTCCGGTG TCTATTAACT TGTCCAAAGC TGAAACTCAG 
GCTTTGACTA ATTATGGAAG TGGAGAAGAT GAGAATGAGG ATGAAGAAAT AGAAGAATTT 
GAAGAGGGAC CTGTGGATGT CCAGACGTCA CTTCAGGCTA ACACTGAAGC TACAGAAGAA 
ACTGAACACG ATGATCAGGT ACTACAACAT GACTTTGAAA AATCAGGAGA AAGCAAAAAT 
GTCCCATCAG AACAAGATCC CACTACTAGT AAAGGCAAGA AATATGACCA AGATAGTACT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAAATGAGC AGCCTTTGAA TAGCGCTGTC 
CAAAAAGACT CTCTGACTAC CATCGATTCA TCTAAACAGC CTAACCCTTT GCCTTTACCT 
TTACCTGAAA TCGAAACCTT GGTGCCTACA GTCAAAGAAG TTAAATCTGC TCAAGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA 
GATTTACCAC TTAAACTGAC AATATACTCA GAGGCAGATC TAAGGAAGAA AATGGTAGAA 
GAGCAAGAGA AAAACCATTT ATCTGGTGAA ATACTATGTG AAATGCAGAC CGAAGAATTA 
GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404161.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259869.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X22, mRNA.

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CloneID	OCe294055
Clone ID Related Accession (Same CDS sequence)	XM_022404161.1
Accession Version	XM_022404161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAGCATGAC 
TTATTAAAAA GGATGTTACA ACAGCAGGAG CAACTGAGAG CTCTGCAGGG AAGACAGGCT 
GCTCTTCTTG CTCTGCAGCA CAAAGCAGAG CAAGCTATTG CTGTGATGGA TGATTCTGTT 
GTAACAGAAA CTACAGGTAG CGTATCTGGA GTTAGTATCA CCTCTGAACT AAATGAAGAA 
TTGAATGATT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAACTGTT 
CCAGACAATA GAAGACAAGC AGAAAGTCTT TCATTAACTA GGGAGGTATC CCAGAGCAGG 
AATCCATCAG TTTCAGAACA CTTACCTGAT GAGAAAGTAC AACTGTTTAG CAAAATGAGA 
GTATTACAGG AAAAGAAACA AAAAATGGAC AAACTACTTG GAGAACTTCA TACACTTCGA 
GATCAACATC TGAACAATTC ATCATTTGTG CCTTCTTCAG CTTCTCCACA AAGAAGTGTG 
GATCAGAGAA GTACAACTTC AGCACCCTCT GCTCCTATAG GCTTAGCACC AGTTGTCAAT 
GGAGAATCCA ACAGCTTCAC ATCATCTGTT CCTTATCCTG TTGCTTCTCT AGTTTCTCAG 
AATGAGAGTG AAAACGAAGG ACATCTAAAT CCAACTGAAA AACTCCAGAA GTTAAATGAA 
GTTCGAAAGA GATTGAATGA ACTAAGAGAA CTAGTTCATT ATTACGAACA AACATCAGAT 
ATGATGACAG ATGCTGTAAA TGAAAACACA AAAGATGAAG AAACTGAAGA GTCAGAATAT 
GATTCTGAAC ATGAAAACCC TGAACCTGTT ACTAACATTC GAAATCCTCA AGTAGCTGCC 
ACTTGGAATG AAGTAAATAG TAATTCTAAT GCACAGTGTG TTTCTAATAA TAGAGAGGGG 
AGATCAGTTA ATTCTAATTG TGAAATTAAC AACAGATCTG CTGCCAACAT AAGGACTCTG 
AACATGCCTC CTTCTTTAGA TTGTCACTAT AATAGAGAAG GAGAACAGGG GATTCATGGT 
GCACAAGGTG AAGACGATGA GGAAGAAGAA GAAGCAGAAG ATGAAGGAGT CAGTGGGGCT 
TCATTAACTA GTCATAGGAG CAGTCTGGTT GATGAGGCTG CAGAAGACGC TGAATTTGAA 
CAGAAGATCA ATAGACTTAT GGCTGCCAAA CAGAAATTAA GACAGTTACA AGATCTTGTT 
GCTATGGTAC AGGATGATGA TGCAGCAGAT CATGGAGTTA TCTCTGCCAA TACTTCAAAT 
TTGGATGATT TTTACCCAGC AGAAGAAGAC AACAAACAAA GTGCAAATAA TACTAGAGGA 
AATGCCAATA AAACACAGAA AGATGCTGGA ATAAATGAGA AGGCAAGAGA GAAGTTTTAT 
GAGGCTAAAC TACAGCAGCA ACAGAGAGAG CTCAGACAAT TGCAGGAAGA AAGAAAGAAA 
CTGATTGAGA TTCAAGAGAA AATTCAAGCA TTACAGAAGG CATGCCCTGA CTTACAGCTG 
TCAGCCACTA GTGCGGGTAA TTGTCCCACA AAAAAATATA TCCCAGCTGT TACTTCAACC 
CCAGTTGTTA ATGGGAATGA AACCAGTACA AGCAAATCTG CTTTTGAGCC TGCAGATCCT 
TCAGGAGTGG ATAATGAGCT GTGGTCAGAA ATGCGAAGAC ATGAGATGTT AAGGGAAGAG 
CTACGGCAGA GAAGAAAGCA GCTTGAAGCA CTGATGGCTG AACATCAGAG GAGGCAAGGT 
CTAGCTGAAA CTACATCCCC TCTGGCTGTG TCATTGAGAA GTGATGGATC TGAGAACCTG 
TGTACGCCTC AACAGAGTAG AACAGAAAAA ACCATGGCAA CATGGGGAGG TTCTACCCAG 
TGTGCACTAG ATGAAGAAGG AGATGAAGAT GGTTACCTTT CTGAAGGAGT TGTTCGGACA 
GATGAAGAGG AGGAAGAAGA AGAGCAAGAT GCAAGTTCGA ATGATAACTT TTCTATGTAT 
CCTCCTAACA GTGCGAATCA TAACTCTTAC AACATAAAAG AAACTAAGAA TAGGTGGAAG 
AATAGTCGCC CTTTTACAGC AGATGGAAAT TATCGTCCTT TAGCCAAGAC AAGACAGCAG 
AATATCAGCA TGCAACGTCA GGAAAACCTT CGTTGGATGT CCGAACTCTC TTACGTAGAG 
GAGAAAGAAC AATGGCAAGA ACAAATTAAT CAGCTGAAGA AACAACTTGA TTTCAGTGTC 
AATATTTGTC AGACTTTGAT GCAAGACCAG CAGACGCTGT CTTGTCTGCT GCAAACTCTT 
CTCACAGGTC CTTACAGTGT TATGCCCAGC AATGTAGCTT CTCCTCAAGT ACACCTTATA 
ATGCACCAGT TGAATCAGTG CTATACTCAG CTAACATGGC AACAGAATAA TGTGCAAAGG 
TTGAAACAAA TGCTAAATGA ACTTATGCGC CAACAGAATC AGCACCCAGA AAAACCTGGA 
AGCCAGGAAA GAGGCAGTAG TGCACCACAG CCTTCTTCTC CCAGTTTATT TTGTCCTTTC 
AGCTTTCCAT CACAGCCTGT AAATCTATTT AACCTACCCG GGTTTACTAA TTTTTCATCA 
TTTGCACCAG GTATGAATTT TAGCCCTTTA TTTCCTTCTA ATTTTGGAGA ATTTTCTCAG 
AATATTTCTA CACCTACTGA ACAGCAGCAA CCATTAGCCC AGAATTCTTC AGGGAAAACA 
GAATATATGG CTTTTCCAAA ACCTTTTGAA AGCAGTTCTT CTATTGGAGC AGAAAAACAA 
AGGAATCAAA AACAGCCTGG AGAGGAGGTG GAAAACAGTA GGACAGCGTG GTTATATGAC 
CAAGAAGGTG AAGTAGAAAA ACCATTTATC AAGACTGGAT TTCCAGTGTC TGTAGAGAAA 
ACTACAAATA GTAATCGCAA AAATCAATTA GATACCGGCC GAAGAAGACG CCAGTTTGAT 
GAAGAGTCAT TAGAAAGCTT TAGCAGTATG CCTGATCCAG TAGATCCGAC AACAGTAACC 
AAAACATTCA AGACGAGAAA AGCATCTGCA CAGGCCAGCC TAGCATCTAA AGATAAAACT 
CCTAAATCAA AGAGTAAGAA AAGGCATTCT GCTCAACTAA AAAGCAGAGT TAAAAATACT 
GGGTATGAAA GTGCCAGTGT GTCTAGCACA TGTGAACCTT GCAAAAGTAG GAACAGACAT 
TCAGCCCAGA CTGAAGAGCC TGTTCAAGCA AAAGTATTCA GCAGAAAGAA TCTTGAGCAG 
CTGGAAAAAA TAATAAAATA TAGTAGGTCT ACAGAGATAT CTTCAGAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGGGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCATCCACA TTTTCTTATT GAACTCTTCC ATGAACTTCA GCTACTTAAT 
ACAGACTACT TGAGACAAAG GGCTTTATAT GCATTGCAGG ACATAGTAAC CAGACATATT 
TCTGAGAACC ATGAAAAAGA AGGAGAAAAT GTAAAGTCAG TGAATTCTGG TACTTGGATA 
GCATCAAACT CAGAGCTTAC CCCCAGTGAA AGCCTTGCTA CCACTGATGA TGAAACTTTT 
GAGAAGAACT TTGAGAGAGA AACACATAAA ATAAGTGAGC AAAATGATGC TGATAATGCT 
AGCGTCATGT CTGTATCTTC AAATTTTGAG CCTTTTGCAA CAGATGATCT AGGTAACACT 
GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGGGAAT ACGAGCGTAT GAAGACTGAG 
GCTGAAAGTA GCACAAATAT AAGATGCACC TGCAGAATTC TTGAGGATGA GGATGGTGCT 
GCCGCCACAA GTATGGTTAC TAATTTAGAA GAAACTCCTA TTGAAAATCA TGGTTCACAA 
CAACCTGTAA GTGAAGTTTC TACTGTTCCA TGTCCTAGAA TTGATACTCA GCAGCTGGAC 
CGGCAAATTA AAGCCATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGAGCA CATGGATGAA 
GTATGCTCTT CTCAACTTCT AACTTCAGTA AGGCGTATGG TTTTGACCCT CACCCAACAA 
AATGATGAGA GCAAAGAATT TGTGAAGTTC TTTCATAAAC AACTTGGAAG TATATTACAG 
GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAAGACT GTGGAGAAGA TCTTCTTGTA 
GAGATATCTG AAGTGTTGTT TAATGAATTG GCTTTCTTTA AGCTCATGCA AGATTTGGAT 
AATAATAGTA TAACTGTTAA ACAGAAGTGC AAAAGGAAAA TAGAAGCAGC TGGAGTGATA 
CAGTCTTATG CCAAAGAGGC CAAAAGGATT CTTGAAGGAG ATCATGGCTC ACCTGCTGGA 
GAAATTGATG ATGAAGACAA AGACAAGGAT GAAACTGAAA CAGTTAAGCC GACTCAGACA 
TCTGAGATAT ATGATGGTGA TGGTCCCAAA AATGTGAGAT CAGATGTTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGA ATGTCCGGTG TCTATTAACT TGTCCAAAGC TGAAACTCAG 
GCTTTGACTA ATTATGGAAG TGGAGAAGAT GAGAATGAGG ATGAAGAAAT AGAAGAATTT 
GAAGAGGGAC CTGTGGATGT CCAGACGTCA CTTCAGGCTA ACACTGAAGC TACAGAAGAA 
ACTGAACACG ATGATCAGGT ACTACAACAT GACTTTGAAA AATCAGGAGA AAGCAAAAAT 
GTCCCATCAG AACAAGATCC CACTACTAGT AAAGGCAAGA AATATGACCA AGATAGTACT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAAATGAGC AGCCTTTGAA TAGCGCTGTC 
CAAAAAGACT CTCTGACTAC CATCGATTCA TCTAAACAGC CTAACCCTTT GCCTTTACCT 
TTACCTGAAA TCGAAACCTT GGTGCCTACA GTCAAAGAAG TTAAATCTGC TCAAGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA 
GATTTACCAC TTAAACTGAC AATATACTCA GAGGCAGATC TAAGGAAGAA AATGGTAGAA 
GAGCAAGAGA AAAACCATTT ATCTGGTGAA ATACTATGTG AAATGCAGAC CGAAGAATTA 
GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259869.1
CDS	426..6662
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLDCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404161.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404161.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAGCATGAC 
TTATTAAAAA GGATGTTACA ACAGCAGGAG CAACTGAGAG CTCTGCAGGG AAGACAGGCT 
GCTCTTCTTG CTCTGCAGCA CAAAGCAGAG CAAGCTATTG CTGTGATGGA TGATTCTGTT 
GTAACAGAAA CTACAGGTAG CGTATCTGGA GTTAGTATCA CCTCTGAACT AAATGAAGAA 
TTGAATGATT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAACTGTT 
CCAGACAATA GAAGACAAGC AGAAAGTCTT TCATTAACTA GGGAGGTATC CCAGAGCAGG 
AATCCATCAG TTTCAGAACA CTTACCTGAT GAGAAAGTAC AACTGTTTAG CAAAATGAGA 
GTATTACAGG AAAAGAAACA AAAAATGGAC AAACTACTTG GAGAACTTCA TACACTTCGA 
GATCAACATC TGAACAATTC ATCATTTGTG CCTTCTTCAG CTTCTCCACA AAGAAGTGTG 
GATCAGAGAA GTACAACTTC AGCACCCTCT GCTCCTATAG GCTTAGCACC AGTTGTCAAT 
GGAGAATCCA ACAGCTTCAC ATCATCTGTT CCTTATCCTG TTGCTTCTCT AGTTTCTCAG 
AATGAGAGTG AAAACGAAGG ACATCTAAAT CCAACTGAAA AACTCCAGAA GTTAAATGAA 
GTTCGAAAGA GATTGAATGA ACTAAGAGAA CTAGTTCATT ATTACGAACA AACATCAGAT 
ATGATGACAG ATGCTGTAAA TGAAAACACA AAAGATGAAG AAACTGAAGA GTCAGAATAT 
GATTCTGAAC ATGAAAACCC TGAACCTGTT ACTAACATTC GAAATCCTCA AGTAGCTGCC 
ACTTGGAATG AAGTAAATAG TAATTCTAAT GCACAGTGTG TTTCTAATAA TAGAGAGGGG 
AGATCAGTTA ATTCTAATTG TGAAATTAAC AACAGATCTG CTGCCAACAT AAGGACTCTG 
AACATGCCTC CTTCTTTAGA TTGTCACTAT AATAGAGAAG GAGAACAGGG GATTCATGGT 
GCACAAGGTG AAGACGATGA GGAAGAAGAA GAAGCAGAAG ATGAAGGAGT CAGTGGGGCT 
TCATTAACTA GTCATAGGAG CAGTCTGGTT GATGAGGCTG CAGAAGACGC TGAATTTGAA 
CAGAAGATCA ATAGACTTAT GGCTGCCAAA CAGAAATTAA GACAGTTACA AGATCTTGTT 
GCTATGGTAC AGGATGATGA TGCAGCAGAT CATGGAGTTA TCTCTGCCAA TACTTCAAAT 
TTGGATGATT TTTACCCAGC AGAAGAAGAC AACAAACAAA GTGCAAATAA TACTAGAGGA 
AATGCCAATA AAACACAGAA AGATGCTGGA ATAAATGAGA AGGCAAGAGA GAAGTTTTAT 
GAGGCTAAAC TACAGCAGCA ACAGAGAGAG CTCAGACAAT TGCAGGAAGA AAGAAAGAAA 
CTGATTGAGA TTCAAGAGAA AATTCAAGCA TTACAGAAGG CATGCCCTGA CTTACAGCTG 
TCAGCCACTA GTGCGGGTAA TTGTCCCACA AAAAAATATA TCCCAGCTGT TACTTCAACC 
CCAGTTGTTA ATGGGAATGA AACCAGTACA AGCAAATCTG CTTTTGAGCC TGCAGATCCT 
TCAGGAGTGG ATAATGAGCT GTGGTCAGAA ATGCGAAGAC ATGAGATGTT AAGGGAAGAG 
CTACGGCAGA GAAGAAAGCA GCTTGAAGCA CTGATGGCTG AACATCAGAG GAGGCAAGGT 
CTAGCTGAAA CTACATCCCC TCTGGCTGTG TCATTGAGAA GTGATGGATC TGAGAACCTG 
TGTACGCCTC AACAGAGTAG AACAGAAAAA ACCATGGCAA CATGGGGAGG TTCTACCCAG 
TGTGCACTAG ATGAAGAAGG AGATGAAGAT GGTTACCTTT CTGAAGGAGT TGTTCGGACA 
GATGAAGAGG AGGAAGAAGA AGAGCAAGAT GCAAGTTCGA ATGATAACTT TTCTATGTAT 
CCTCCTAACA GTGCGAATCA TAACTCTTAC AACATAAAAG AAACTAAGAA TAGGTGGAAG 
AATAGTCGCC CTTTTACAGC AGATGGAAAT TATCGTCCTT TAGCCAAGAC AAGACAGCAG 
AATATCAGCA TGCAACGTCA GGAAAACCTT CGTTGGATGT CCGAACTCTC TTACGTAGAG 
GAGAAAGAAC AATGGCAAGA ACAAATTAAT CAGCTGAAGA AACAACTTGA TTTCAGTGTC 
AATATTTGTC AGACTTTGAT GCAAGACCAG CAGACGCTGT CTTGTCTGCT GCAAACTCTT 
CTCACAGGTC CTTACAGTGT TATGCCCAGC AATGTAGCTT CTCCTCAAGT ACACCTTATA 
ATGCACCAGT TGAATCAGTG CTATACTCAG CTAACATGGC AACAGAATAA TGTGCAAAGG 
TTGAAACAAA TGCTAAATGA ACTTATGCGC CAACAGAATC AGCACCCAGA AAAACCTGGA 
AGCCAGGAAA GAGGCAGTAG TGCACCACAG CCTTCTTCTC CCAGTTTATT TTGTCCTTTC 
AGCTTTCCAT CACAGCCTGT AAATCTATTT AACCTACCCG GGTTTACTAA TTTTTCATCA 
TTTGCACCAG GTATGAATTT TAGCCCTTTA TTTCCTTCTA ATTTTGGAGA ATTTTCTCAG 
AATATTTCTA CACCTACTGA ACAGCAGCAA CCATTAGCCC AGAATTCTTC AGGGAAAACA 
GAATATATGG CTTTTCCAAA ACCTTTTGAA AGCAGTTCTT CTATTGGAGC AGAAAAACAA 
AGGAATCAAA AACAGCCTGG AGAGGAGGTG GAAAACAGTA GGACAGCGTG GTTATATGAC 
CAAGAAGGTG AAGTAGAAAA ACCATTTATC AAGACTGGAT TTCCAGTGTC TGTAGAGAAA 
ACTACAAATA GTAATCGCAA AAATCAATTA GATACCGGCC GAAGAAGACG CCAGTTTGAT 
GAAGAGTCAT TAGAAAGCTT TAGCAGTATG CCTGATCCAG TAGATCCGAC AACAGTAACC 
AAAACATTCA AGACGAGAAA AGCATCTGCA CAGGCCAGCC TAGCATCTAA AGATAAAACT 
CCTAAATCAA AGAGTAAGAA AAGGCATTCT GCTCAACTAA AAAGCAGAGT TAAAAATACT 
GGGTATGAAA GTGCCAGTGT GTCTAGCACA TGTGAACCTT GCAAAAGTAG GAACAGACAT 
TCAGCCCAGA CTGAAGAGCC TGTTCAAGCA AAAGTATTCA GCAGAAAGAA TCTTGAGCAG 
CTGGAAAAAA TAATAAAATA TAGTAGGTCT ACAGAGATAT CTTCAGAAAC TGGGAGTGAT 
TTTTCCATGT TTGAAGCTTT GCGGGATACT ATTTATTCTG AAGTAGCTAC ATTAATTTCT 
CAAAATGAAT CTCATCCACA TTTTCTTATT GAACTCTTCC ATGAACTTCA GCTACTTAAT 
ACAGACTACT TGAGACAAAG GGCTTTATAT GCATTGCAGG ACATAGTAAC CAGACATATT 
TCTGAGAACC ATGAAAAAGA AGGAGAAAAT GTAAAGTCAG TGAATTCTGG TACTTGGATA 
GCATCAAACT CAGAGCTTAC CCCCAGTGAA AGCCTTGCTA CCACTGATGA TGAAACTTTT 
GAGAAGAACT TTGAGAGAGA AACACATAAA ATAAGTGAGC AAAATGATGC TGATAATGCT 
AGCGTCATGT CTGTATCTTC AAATTTTGAG CCTTTTGCAA CAGATGATCT AGGTAACACT 
GTGATTCACT TAGATCAGGC ATTAGCCAGA ATGAGGGAAT ACGAGCGTAT GAAGACTGAG 
GCTGAAAGTA GCACAAATAT AAGATGCACC TGCAGAATTC TTGAGGATGA GGATGGTGCT 
GCCGCCACAA GTATGGTTAC TAATTTAGAA GAAACTCCTA TTGAAAATCA TGGTTCACAA 
CAACCTGTAA GTGAAGTTTC TACTGTTCCA TGTCCTAGAA TTGATACTCA GCAGCTGGAC 
CGGCAAATTA AAGCCATTAT GAAAGAAGTC ATTCCTTTTT TGAAGGAGCA CATGGATGAA 
GTATGCTCTT CTCAACTTCT AACTTCAGTA AGGCGTATGG TTTTGACCCT CACCCAACAA 
AATGATGAGA GCAAAGAATT TGTGAAGTTC TTTCATAAAC AACTTGGAAG TATATTACAG 
GATTCACTGG CAAAATTTGC TGGCAGAAAA CTGAAAGACT GTGGAGAAGA TCTTCTTGTA 
GAGATATCTG AAGTGTTGTT TAATGAATTG GCTTTCTTTA AGCTCATGCA AGATTTGGAT 
AATAATAGTA TAACTGTTAA ACAGAAGTGC AAAAGGAAAA TAGAAGCAGC TGGAGTGATA 
CAGTCTTATG CCAAAGAGGC CAAAAGGATT CTTGAAGGAG ATCATGGCTC ACCTGCTGGA 
GAAATTGATG ATGAAGACAA AGACAAGGAT GAAACTGAAA CAGTTAAGCC GACTCAGACA 
TCTGAGATAT ATGATGGTGA TGGTCCCAAA AATGTGAGAT CAGATGTTTC TGATCAAGAG 
GAAGATGAAG AAAGTGAAGA ATGTCCGGTG TCTATTAACT TGTCCAAAGC TGAAACTCAG 
GCTTTGACTA ATTATGGAAG TGGAGAAGAT GAGAATGAGG ATGAAGAAAT AGAAGAATTT 
GAAGAGGGAC CTGTGGATGT CCAGACGTCA CTTCAGGCTA ACACTGAAGC TACAGAAGAA 
ACTGAACACG ATGATCAGGT ACTACAACAT GACTTTGAAA AATCAGGAGA AAGCAAAAAT 
GTCCCATCAG AACAAGATCC CACTACTAGT AAAGGCAAGA AATATGACCA AGATAGTACT 
CCTGTGAAAC CCTGTTACCT CAATATCTTG GAAAATGAGC AGCCTTTGAA TAGCGCTGTC 
CAAAAAGACT CTCTGACTAC CATCGATTCA TCTAAACAGC CTAACCCTTT GCCTTTACCT 
TTACCTGAAA TCGAAACCTT GGTGCCTACA GTCAAAGAAG TTAAATCTGC TCAAGAAACT 
CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT ACTGAATCTC CAGTGTTAGT GAATGACTAT 
GAAGCAGAAT CTGGTAACAT AAGTCAAAAA TCTGATGAAG AAGACTTTGT GAAAGTTGAA 
GATTTACCAC TTAAACTGAC AATATACTCA GAGGCAGATC TAAGGAAGAA AATGGTAGAA 
GAGCAAGAGA AAAACCATTT ATCTGGTGAA ATACTATGTG AAATGCAGAC CGAAGAATTA 
GCTGGGAATT CTCAGACACT AAAAGAACCT GAAACAGTGG GAGCCCAAAG CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404163.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259871.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X23, mRNA.

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CloneID	OCe294056
Clone ID Related Accession (Same CDS sequence)	XM_022404163.1
Accession Version	XM_022404163.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAGCATGAC 
TTATTAAAAA GGATGTTACA ACAGCAGGAG CAACTGAGAG CTCTGCAGGG AAGACAGGCT 
GCTCTTCTTG CTCTGCAGCA CAAAGCAGAG CAAGCTATTG CTGTGATGGA TGATTCTGTT 
GTAACAGAAA CTACAGGTAG CGTATCTGGA GTTAGTATCA CCTCTGAACT AAATGAAGAA 
TTGAATGATT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAACTGTT 
CCAGACAATA GAAGACAAGC AGAAAGTCTT TCATTAACTA GGGAGGTATC CCAGAGCAGG 
AATCCATCAG TTTCAGAACA CTTACCTGAT GAGAAAGTAC AACTGTTTAG CAAAATGAGA 
GTATTACAGG AAAAGAAACA AAAAATGGAC AAACTACTTG GAGAACTTCA TACACTTCGA 
GATCAACATC TGAACAATTC ATCATTTGTG CCTTCTTCAG CTTCTCCACA AAGAAGTGTG 
GATCAGAGAA GTACAACTTC AGCACCCTCT GCTCCTATAG GCTTAGCACC AGTTGTCAAT 
GGAGAATCCA ACAGCTTCAC ATCATCTGTT CCTTATCCTG TTGCTTCTCT AGTTTCTCAG 
AATGAGAGTG AAAACGAAGG ACATCTAAAT CCAACTGAAA AACTCCAGAA GTTAAATGAA 
GTTCGAAAGA GATTGAATGA ACTAAGAGAA CTAGTTCATT ATTACGAACA AACATCAGAT 
ATGATGACAG ATGCTGTAAA TGAAAACACA AAAGATGAAG AAACTGAAGA GTCAGAATAT 
GATTCTGAAC ATGAAAACCC TGAACCTGTT ACTAACATTC GAAATCCTCA AGTAGCTGCC 
ACTTGGAATG AAGTAAATAG TAATTCTAAT GCACAGTGTG TTTCTAATAA TAGAGAGGGG 
AGATCAGTTA ATTCTAATTG TGAAATTAAC AACAGATCTG CTGCCAACAT AAGGACTCTG 
AACATGCCTC CTTCTTTAGC AGATTGTCAC TATAATAGAG AAGGAGAACA GGGGATTCAT 
GGTGCACAAG GTGAAGACGA TGAGGAAGAA GAAGAAGCAG AAGATGAAGG AGTCAGTGGG 
GCTTCATTAA CTAGTCATAG GAGCAGTCTG GTTGATGAGG CTGCAGAAGA CGCTGAATTT 
GAACAGAAGA TCAATAGACT TATGGCTGCC AAACAGAAAT TAAGACAGTT ACAAGATCTT 
GTTGCTATGG TACAGGATGA TGATGCAGCA GATCATGGAG TTATCTCTGC CAATACTTCA 
AATTTGGATG ATTTTTACCC AGCAGAAGAA GACAACAAAC AAAGTGCAAA TAATACTAGA 
GGAAATGCCA ATAAAACACA GAAAGATGCT GGAATAAATG AGAAGGCAAG AGAGAAGTTT 
TATGAGGCTA AACTACAGCA GCAACAGAGA GAGCTCAGAC AATTGCAGGA AGAAAGAAAG 
AAACTGATTG AGATTCAAGA GAAAATTCAA GCATTACAGA AGGCATGCCC TGACTTACAG 
CTGTCAGCCA CTAGTGCGGG TAATTGTCCC ACAAAAAAAT ATATCCCAGC TGTTACTTCA 
ACCCCAGTTG TTAATGGGAA TGAAACCAGT ACAAGCAAAT CTGCTTTTGA GCCTGCAGAT 
CCTTCAGGAG TGGATAATGA GCTGTGGTCA GAAATGCGAA GACATGAGAT GTTAAGGGAA 
GAGCTACGGC AGAGAAGAAA GCAGCTTGAA GCACTGATGG CTGAACATCA GAGGAGGCAA 
GGTCTAGCTG AAACTACATC CCCTCTGGCT GTGTCATTGA GAAGTGATGG ATCTGAGAAC 
CTGTGTACGC CTCAACAGAG TAGAACAGAA AAAACCATGG CAACATGGGG AGGTTCTACC 
CAGTGTGCAC TAGATGAAGA AGGAGATGAA GATGGTTACC TTTCTGAAGG AGTTGTTCGG 
ACAGATGAAG AGGAGGAAGA AGAAGAGCAA GATGCAAGTT CGAATGATAA CTTTTCTATG 
TATCCTCCTA ACAGTGCGAA TCATAACTCT TACAACATAA AAGAAACTAA GAATAGGTGG 
AAGAATAGTC GCCCTTTTAC AGCAGATGGA AATTATCGTC CTTTAGCCAA GACAAGACAG 
CAGAATATCA GCATGCAACG TCAGGAAAAC CTTCGTTGGA TGTCCGAACT CTCTTACGTA 
GAGGAGAAAG AACAATGGCA AGAACAAATT AATCAGCTGA AGAAACAACT TGATTTCAGT 
GTCAATATTT GTCAGACTTT GATGCAAGAC CAGCAGACGC TGTCTTGTCT GCTGCAAACT 
CTTCTCACAG GTCCTTACAG TGTTATGCCC AGCAATGTAG CTTCTCCTCA AGTACACCTT 
ATAATGCACC AGTTGAATCA GTGCTATACT CAGCTAACAT GGCAACAGAA TAATGTGCAA 
AGGTTGAAAC AAATGCTAAA TGAACTTATG CGCCAACAGA ATCAGCACCC AGAAAAACCT 
GGAAGCCAGG AAAGAGGCAG TAGTGCACCA CAGCCTTCTT CTCCCAGTTT ATTTTGTCCT 
TTCAGCTTTC CATCACAGCC TGTAAATCTA TTTAACCTAC CCGGGTTTAC TAATTTTTCA 
TCATTTGCAC CAGGTATGAA TTTTAGCCCT TTATTTCCTT CTAATTTTGG AGAATTTTCT 
CAGAATATTT CTACACCTAC TGAACAGCAG CAACCATTAG CCCAGAATTC TTCAGGGAAA 
ACAGAATATA TGGCTTTTCC AAAACCTTTT GAAAGCAGTT CTTCTATTGG AGCAGAAAAA 
CAAAGGAATC AAAAACAGCC TGGAGAGGAG GTGGAAAACA GTAGGACAGC GTGGTTATAT 
GACCAAGAAG GTGAAGTAGA AAAACCATTT ATCAAGACTG GATTTCCAGT GTCTGTAGAG 
AAAACTACAA ATAGTAATCG CAAAAATCAA TTAGATACCG GCCGAAGAAG ACGCCAGTTT 
GATGAAGAGT CATTAGAAAG CTTTAGCAGT ATGCCTGATC CAGTAGATCC GACAACAGTA 
ACCAAAACAT TCAAGACGAG AAAAGCATCT GCACAGGCCA GCCTAGCATC TAAAGATAAA 
ACTCCTAAAT CAAAGAGTAA GAAAAGGCAT TCTGCTCAAC TAAAAAGCAG AGTTAAAAAT 
ACTGAAACTG GGAGTGATTT TTCCATGTTT GAAGCTTTGC GGGATACTAT TTATTCTGAA 
GTAGCTACAT TAATTTCTCA AAATGAATCT CATCCACATT TTCTTATTGA ACTCTTCCAT 
GAACTTCAGC TACTTAATAC AGACTACTTG AGACAAAGGG CTTTATATGC ATTGCAGGAC 
ATAGTAACCA GACATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATGT AAAGTCAGTG 
AATTCTGGTA CTTGGATAGC ATCAAACTCA GAGCTTACCC CCAGTGAAAG CCTTGCTACC 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG CGTCATGTCT GTATCTTCAA ATTTTGAGCC TTTTGCAACA 
GATGATCTAG GTAACACTGT GATTCACTTA GATCAGGCAT TAGCCAGAAT GAGGGAATAC 
GAGCGTATGA AGACTGAGGC TGAAAGTAGC ACAAATATAA GATGCACCTG CAGAATTCTT 
GAGGATGAGG ATGGTGCTGC CGCCACAAGT ATGGTTACTA ATTTAGAAGA AACTCCTATT 
GAAAATCATG GTTCACAACA ACCTGTAAGT GAAGTTTCTA CTGTTCCATG TCCTAGAATT 
GATACTCAGC AGCTGGACCG GCAAATTAAA GCCATTATGA AAGAAGTCAT TCCTTTTTTG 
AAGGAGCACA TGGATGAAGT ATGCTCTTCT CAACTTCTAA CTTCAGTAAG GCGTATGGTT 
TTGACCCTCA CCCAACAAAA TGATGAGAGC AAAGAATTTG TGAAGTTCTT TCATAAACAA 
CTTGGAAGTA TATTACAGGA TTCACTGGCA AAATTTGCTG GCAGAAAACT GAAAGACTGT 
GGAGAAGATC TTCTTGTAGA GATATCTGAA GTGTTGTTTA ATGAATTGGC TTTCTTTAAG 
CTCATGCAAG ATTTGGATAA TAATAGTATA ACTGTTAAAC AGAAGTGCAA AAGGAAAATA 
GAAGCAGCTG GAGTGATACA GTCTTATGCC AAAGAGGCCA AAAGGATTCT TGAAGGAGAT 
CATGGCTCAC CTGCTGGAGA AATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCCGA CTCAGACATC TGAGATATAT GATGGTGATG GTCCCAAAAA TGTGAGATCA 
GATGTTTCTG ATCAAGAGGA AGATGAAGAA AGTGAAGAAT GTCCGGTGTC TATTAACTTG 
TCCAAAGCTG AAACTCAGGC TTTGACTAAT TATGGAAGTG GAGAAGATGA GAATGAGGAT 
GAAGAAATAG AAGAATTTGA AGAGGGACCT GTGGATGTCC AGACGTCACT TCAGGCTAAC 
ACTGAAGCTA CAGAAGAAAC TGAACACGAT GATCAGGTAC TACAACATGA CTTTGAAAAA 
TCAGGAGAAA GCAAAAATGT CCCATCAGAA CAAGATCCCA CTACTAGTAA AGGCAAGAAA 
TATGACCAAG ATAGTACTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AAATGAGCAG 
CCTTTGAATA GCGCTGTCCA AAAAGACTCT CTGACTACCA TCGATTCATC TAAACAGCCT 
AACCCTTTGC CTTTACCTTT ACCTGAAATC GAAACCTTGG TGCCTACAGT CAAAGAAGTT 
AAATCTGCTC AAGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA 
GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATACTCAGA GGCAGATCTA 
AGGAAGAAAA TGGTAGAAGA GCAAGAGAAA AACCATTTAT CTGGTGAAAT ACTATGTGAA 
ATGCAGACCG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA 
GCCCAAAGCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259871.1
CDS	426..6500
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404163.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404163.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAGCATGAC 
TTATTAAAAA GGATGTTACA ACAGCAGGAG CAACTGAGAG CTCTGCAGGG AAGACAGGCT 
GCTCTTCTTG CTCTGCAGCA CAAAGCAGAG CAAGCTATTG CTGTGATGGA TGATTCTGTT 
GTAACAGAAA CTACAGGTAG CGTATCTGGA GTTAGTATCA CCTCTGAACT AAATGAAGAA 
TTGAATGATT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAACTGTT 
CCAGACAATA GAAGACAAGC AGAAAGTCTT TCATTAACTA GGGAGGTATC CCAGAGCAGG 
AATCCATCAG TTTCAGAACA CTTACCTGAT GAGAAAGTAC AACTGTTTAG CAAAATGAGA 
GTATTACAGG AAAAGAAACA AAAAATGGAC AAACTACTTG GAGAACTTCA TACACTTCGA 
GATCAACATC TGAACAATTC ATCATTTGTG CCTTCTTCAG CTTCTCCACA AAGAAGTGTG 
GATCAGAGAA GTACAACTTC AGCACCCTCT GCTCCTATAG GCTTAGCACC AGTTGTCAAT 
GGAGAATCCA ACAGCTTCAC ATCATCTGTT CCTTATCCTG TTGCTTCTCT AGTTTCTCAG 
AATGAGAGTG AAAACGAAGG ACATCTAAAT CCAACTGAAA AACTCCAGAA GTTAAATGAA 
GTTCGAAAGA GATTGAATGA ACTAAGAGAA CTAGTTCATT ATTACGAACA AACATCAGAT 
ATGATGACAG ATGCTGTAAA TGAAAACACA AAAGATGAAG AAACTGAAGA GTCAGAATAT 
GATTCTGAAC ATGAAAACCC TGAACCTGTT ACTAACATTC GAAATCCTCA AGTAGCTGCC 
ACTTGGAATG AAGTAAATAG TAATTCTAAT GCACAGTGTG TTTCTAATAA TAGAGAGGGG 
AGATCAGTTA ATTCTAATTG TGAAATTAAC AACAGATCTG CTGCCAACAT AAGGACTCTG 
AACATGCCTC CTTCTTTAGC AGATTGTCAC TATAATAGAG AAGGAGAACA GGGGATTCAT 
GGTGCACAAG GTGAAGACGA TGAGGAAGAA GAAGAAGCAG AAGATGAAGG AGTCAGTGGG 
GCTTCATTAA CTAGTCATAG GAGCAGTCTG GTTGATGAGG CTGCAGAAGA CGCTGAATTT 
GAACAGAAGA TCAATAGACT TATGGCTGCC AAACAGAAAT TAAGACAGTT ACAAGATCTT 
GTTGCTATGG TACAGGATGA TGATGCAGCA GATCATGGAG TTATCTCTGC CAATACTTCA 
AATTTGGATG ATTTTTACCC AGCAGAAGAA GACAACAAAC AAAGTGCAAA TAATACTAGA 
GGAAATGCCA ATAAAACACA GAAAGATGCT GGAATAAATG AGAAGGCAAG AGAGAAGTTT 
TATGAGGCTA AACTACAGCA GCAACAGAGA GAGCTCAGAC AATTGCAGGA AGAAAGAAAG 
AAACTGATTG AGATTCAAGA GAAAATTCAA GCATTACAGA AGGCATGCCC TGACTTACAG 
CTGTCAGCCA CTAGTGCGGG TAATTGTCCC ACAAAAAAAT ATATCCCAGC TGTTACTTCA 
ACCCCAGTTG TTAATGGGAA TGAAACCAGT ACAAGCAAAT CTGCTTTTGA GCCTGCAGAT 
CCTTCAGGAG TGGATAATGA GCTGTGGTCA GAAATGCGAA GACATGAGAT GTTAAGGGAA 
GAGCTACGGC AGAGAAGAAA GCAGCTTGAA GCACTGATGG CTGAACATCA GAGGAGGCAA 
GGTCTAGCTG AAACTACATC CCCTCTGGCT GTGTCATTGA GAAGTGATGG ATCTGAGAAC 
CTGTGTACGC CTCAACAGAG TAGAACAGAA AAAACCATGG CAACATGGGG AGGTTCTACC 
CAGTGTGCAC TAGATGAAGA AGGAGATGAA GATGGTTACC TTTCTGAAGG AGTTGTTCGG 
ACAGATGAAG AGGAGGAAGA AGAAGAGCAA GATGCAAGTT CGAATGATAA CTTTTCTATG 
TATCCTCCTA ACAGTGCGAA TCATAACTCT TACAACATAA AAGAAACTAA GAATAGGTGG 
AAGAATAGTC GCCCTTTTAC AGCAGATGGA AATTATCGTC CTTTAGCCAA GACAAGACAG 
CAGAATATCA GCATGCAACG TCAGGAAAAC CTTCGTTGGA TGTCCGAACT CTCTTACGTA 
GAGGAGAAAG AACAATGGCA AGAACAAATT AATCAGCTGA AGAAACAACT TGATTTCAGT 
GTCAATATTT GTCAGACTTT GATGCAAGAC CAGCAGACGC TGTCTTGTCT GCTGCAAACT 
CTTCTCACAG GTCCTTACAG TGTTATGCCC AGCAATGTAG CTTCTCCTCA AGTACACCTT 
ATAATGCACC AGTTGAATCA GTGCTATACT CAGCTAACAT GGCAACAGAA TAATGTGCAA 
AGGTTGAAAC AAATGCTAAA TGAACTTATG CGCCAACAGA ATCAGCACCC AGAAAAACCT 
GGAAGCCAGG AAAGAGGCAG TAGTGCACCA CAGCCTTCTT CTCCCAGTTT ATTTTGTCCT 
TTCAGCTTTC CATCACAGCC TGTAAATCTA TTTAACCTAC CCGGGTTTAC TAATTTTTCA 
TCATTTGCAC CAGGTATGAA TTTTAGCCCT TTATTTCCTT CTAATTTTGG AGAATTTTCT 
CAGAATATTT CTACACCTAC TGAACAGCAG CAACCATTAG CCCAGAATTC TTCAGGGAAA 
ACAGAATATA TGGCTTTTCC AAAACCTTTT GAAAGCAGTT CTTCTATTGG AGCAGAAAAA 
CAAAGGAATC AAAAACAGCC TGGAGAGGAG GTGGAAAACA GTAGGACAGC GTGGTTATAT 
GACCAAGAAG GTGAAGTAGA AAAACCATTT ATCAAGACTG GATTTCCAGT GTCTGTAGAG 
AAAACTACAA ATAGTAATCG CAAAAATCAA TTAGATACCG GCCGAAGAAG ACGCCAGTTT 
GATGAAGAGT CATTAGAAAG CTTTAGCAGT ATGCCTGATC CAGTAGATCC GACAACAGTA 
ACCAAAACAT TCAAGACGAG AAAAGCATCT GCACAGGCCA GCCTAGCATC TAAAGATAAA 
ACTCCTAAAT CAAAGAGTAA GAAAAGGCAT TCTGCTCAAC TAAAAAGCAG AGTTAAAAAT 
ACTGAAACTG GGAGTGATTT TTCCATGTTT GAAGCTTTGC GGGATACTAT TTATTCTGAA 
GTAGCTACAT TAATTTCTCA AAATGAATCT CATCCACATT TTCTTATTGA ACTCTTCCAT 
GAACTTCAGC TACTTAATAC AGACTACTTG AGACAAAGGG CTTTATATGC ATTGCAGGAC 
ATAGTAACCA GACATATTTC TGAGAACCAT GAAAAAGAAG GAGAAAATGT AAAGTCAGTG 
AATTCTGGTA CTTGGATAGC ATCAAACTCA GAGCTTACCC CCAGTGAAAG CCTTGCTACC 
ACTGATGATG AAACTTTTGA GAAGAACTTT GAGAGAGAAA CACATAAAAT AAGTGAGCAA 
AATGATGCTG ATAATGCTAG CGTCATGTCT GTATCTTCAA ATTTTGAGCC TTTTGCAACA 
GATGATCTAG GTAACACTGT GATTCACTTA GATCAGGCAT TAGCCAGAAT GAGGGAATAC 
GAGCGTATGA AGACTGAGGC TGAAAGTAGC ACAAATATAA GATGCACCTG CAGAATTCTT 
GAGGATGAGG ATGGTGCTGC CGCCACAAGT ATGGTTACTA ATTTAGAAGA AACTCCTATT 
GAAAATCATG GTTCACAACA ACCTGTAAGT GAAGTTTCTA CTGTTCCATG TCCTAGAATT 
GATACTCAGC AGCTGGACCG GCAAATTAAA GCCATTATGA AAGAAGTCAT TCCTTTTTTG 
AAGGAGCACA TGGATGAAGT ATGCTCTTCT CAACTTCTAA CTTCAGTAAG GCGTATGGTT 
TTGACCCTCA CCCAACAAAA TGATGAGAGC AAAGAATTTG TGAAGTTCTT TCATAAACAA 
CTTGGAAGTA TATTACAGGA TTCACTGGCA AAATTTGCTG GCAGAAAACT GAAAGACTGT 
GGAGAAGATC TTCTTGTAGA GATATCTGAA GTGTTGTTTA ATGAATTGGC TTTCTTTAAG 
CTCATGCAAG ATTTGGATAA TAATAGTATA ACTGTTAAAC AGAAGTGCAA AAGGAAAATA 
GAAGCAGCTG GAGTGATACA GTCTTATGCC AAAGAGGCCA AAAGGATTCT TGAAGGAGAT 
CATGGCTCAC CTGCTGGAGA AATTGATGAT GAAGACAAAG ACAAGGATGA AACTGAAACA 
GTTAAGCCGA CTCAGACATC TGAGATATAT GATGGTGATG GTCCCAAAAA TGTGAGATCA 
GATGTTTCTG ATCAAGAGGA AGATGAAGAA AGTGAAGAAT GTCCGGTGTC TATTAACTTG 
TCCAAAGCTG AAACTCAGGC TTTGACTAAT TATGGAAGTG GAGAAGATGA GAATGAGGAT 
GAAGAAATAG AAGAATTTGA AGAGGGACCT GTGGATGTCC AGACGTCACT TCAGGCTAAC 
ACTGAAGCTA CAGAAGAAAC TGAACACGAT GATCAGGTAC TACAACATGA CTTTGAAAAA 
TCAGGAGAAA GCAAAAATGT CCCATCAGAA CAAGATCCCA CTACTAGTAA AGGCAAGAAA 
TATGACCAAG ATAGTACTCC TGTGAAACCC TGTTACCTCA ATATCTTGGA AAATGAGCAG 
CCTTTGAATA GCGCTGTCCA AAAAGACTCT CTGACTACCA TCGATTCATC TAAACAGCCT 
AACCCTTTGC CTTTACCTTT ACCTGAAATC GAAACCTTGG TGCCTACAGT CAAAGAAGTT 
AAATCTGCTC AAGAAACTCC TGAAAGCTCT CTGGCTGGAA GTCCTGATAC TGAATCTCCA 
GTGTTAGTGA ATGACTATGA AGCAGAATCT GGTAACATAA GTCAAAAATC TGATGAAGAA 
GACTTTGTGA AAGTTGAAGA TTTACCACTT AAACTGACAA TATACTCAGA GGCAGATCTA 
AGGAAGAAAA TGGTAGAAGA GCAAGAGAAA AACCATTTAT CTGGTGAAAT ACTATGTGAA 
ATGCAGACCG AAGAATTAGC TGGGAATTCT CAGACACTAA AAGAACCTGA AACAGTGGGA 
GCCCAAAGCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404164.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259872.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X24, mRNA.

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CloneID	OCe294057
Clone ID Related Accession (Same CDS sequence)	XM_022404164.1
Accession Version	XM_022404164.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAGCATGAC 
TTATTAAAAA GGATGTTACA ACAGCAGGAG CAACTGAGAG CTCTGCAGGG AAGACAGGCT 
GCTCTTCTTG CTCTGCAGCA CAAAGCAGAG CAAGCTATTG CTGTGATGGA TGATTCTGTT 
GTAACAGAAA CTACAGGTAG CGTATCTGGA GTTAGTATCA CCTCTGAACT AAATGAAGAA 
TTGAATGATT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAACTGTT 
CCAGACAATA GAAGACAAGC AGAAAGTCTT TCATTAACTA GGGAGGTATC CCAGAGCAGG 
AATCCATCAG TTTCAGAACA CTTACCTGAT GAGAAAGTAC AACTGTTTAG CAAAATGAGA 
GTATTACAGG AAAAGAAACA AAAAATGGAC AAACTACTTG GAGAACTTCA TACACTTCGA 
GATCAACATC TGAACAATTC ATCATTTGTG CCTTCTTCAG CTTCTCCACA AAGAAGTGTG 
GATCAGAGAA GTACAACTTC AGCACCCTCT GCTCCTATAG GCTTAGCACC AGTTGTCAAT 
GGAGAATCCA ACAGCTTCAC ATCATCTGTT CCTTATCCTG TTGCTTCTCT AGTTTCTCAG 
AATGAGAGTG AAAACGAAGG ACATCTAAAT CCAACTGAAA AACTCCAGAA GTTAAATGAA 
GTTCGAAAGA GATTGAATGA ACTAAGAGAA CTAGTTCATT ATTACGAACA AACATCAGAT 
ATGATGACAG ATGCTGTAAA TGAAAACACA AAAGATGAAG AAACTGAAGA GTCAGAATAT 
GATTCTGAAC ATGAAAACCC TGAACCTGTT ACTAACATTC GAAATCCTCA AGTAGCTGCC 
ACTTGGAATG AAGTAAATAG TAATTCTAAT GCACAGTGTG TTTCTAATAA TAGAGAGGGG 
AGATCAGTTA ATTCTAATTG TGAAATTAAC AACAGATCTG CTGCCAACAT AAGGACTCTG 
AACATGCCTC CTTCTTTAGA TTGTCACTAT AATAGAGAAG GAGAACAGGG GATTCATGGT 
GCACAAGGTG AAGACGATGA GGAAGAAGAA GAAGCAGAAG ATGAAGGAGT CAGTGGGGCT 
TCATTAACTA GTCATAGGAG CAGTCTGGTT GATGAGGCTG CAGAAGACGC TGAATTTGAA 
CAGAAGATCA ATAGACTTAT GGCTGCCAAA CAGAAATTAA GACAGTTACA AGATCTTGTT 
GCTATGGTAC AGGATGATGA TGCAGCAGAT CATGGAGTTA TCTCTGCCAA TACTTCAAAT 
TTGGATGATT TTTACCCAGC AGAAGAAGAC AACAAACAAA GTGCAAATAA TACTAGAGGA 
AATGCCAATA AAACACAGAA AGATGCTGGA ATAAATGAGA AGGCAAGAGA GAAGTTTTAT 
GAGGCTAAAC TACAGCAGCA ACAGAGAGAG CTCAGACAAT TGCAGGAAGA AAGAAAGAAA 
CTGATTGAGA TTCAAGAGAA AATTCAAGCA TTACAGAAGG CATGCCCTGA CTTACAGCTG 
TCAGCCACTA GTGCGGGTAA TTGTCCCACA AAAAAATATA TCCCAGCTGT TACTTCAACC 
CCAGTTGTTA ATGGGAATGA AACCAGTACA AGCAAATCTG CTTTTGAGCC TGCAGATCCT 
TCAGGAGTGG ATAATGAGCT GTGGTCAGAA ATGCGAAGAC ATGAGATGTT AAGGGAAGAG 
CTACGGCAGA GAAGAAAGCA GCTTGAAGCA CTGATGGCTG AACATCAGAG GAGGCAAGGT 
CTAGCTGAAA CTACATCCCC TCTGGCTGTG TCATTGAGAA GTGATGGATC TGAGAACCTG 
TGTACGCCTC AACAGAGTAG AACAGAAAAA ACCATGGCAA CATGGGGAGG TTCTACCCAG 
TGTGCACTAG ATGAAGAAGG AGATGAAGAT GGTTACCTTT CTGAAGGAGT TGTTCGGACA 
GATGAAGAGG AGGAAGAAGA AGAGCAAGAT GCAAGTTCGA ATGATAACTT TTCTATGTAT 
CCTCCTAACA GTGCGAATCA TAACTCTTAC AACATAAAAG AAACTAAGAA TAGGTGGAAG 
AATAGTCGCC CTTTTACAGC AGATGGAAAT TATCGTCCTT TAGCCAAGAC AAGACAGCAG 
AATATCAGCA TGCAACGTCA GGAAAACCTT CGTTGGATGT CCGAACTCTC TTACGTAGAG 
GAGAAAGAAC AATGGCAAGA ACAAATTAAT CAGCTGAAGA AACAACTTGA TTTCAGTGTC 
AATATTTGTC AGACTTTGAT GCAAGACCAG CAGACGCTGT CTTGTCTGCT GCAAACTCTT 
CTCACAGGTC CTTACAGTGT TATGCCCAGC AATGTAGCTT CTCCTCAAGT ACACCTTATA 
ATGCACCAGT TGAATCAGTG CTATACTCAG CTAACATGGC AACAGAATAA TGTGCAAAGG 
TTGAAACAAA TGCTAAATGA ACTTATGCGC CAACAGAATC AGCACCCAGA AAAACCTGGA 
AGCCAGGAAA GAGGCAGTAG TGCACCACAG CCTTCTTCTC CCAGTTTATT TTGTCCTTTC 
AGCTTTCCAT CACAGCCTGT AAATCTATTT AACCTACCCG GGTTTACTAA TTTTTCATCA 
TTTGCACCAG GTATGAATTT TAGCCCTTTA TTTCCTTCTA ATTTTGGAGA ATTTTCTCAG 
AATATTTCTA CACCTACTGA ACAGCAGCAA CCATTAGCCC AGAATTCTTC AGGGAAAACA 
GAATATATGG CTTTTCCAAA ACCTTTTGAA AGCAGTTCTT CTATTGGAGC AGAAAAACAA 
AGGAATCAAA AACAGCCTGG AGAGGAGGTG GAAAACAGTA GGACAGCGTG GTTATATGAC 
CAAGAAGGTG AAGTAGAAAA ACCATTTATC AAGACTGGAT TTCCAGTGTC TGTAGAGAAA 
ACTACAAATA GTAATCGCAA AAATCAATTA GATACCGGCC GAAGAAGACG CCAGTTTGAT 
GAAGAGTCAT TAGAAAGCTT TAGCAGTATG CCTGATCCAG TAGATCCGAC AACAGTAACC 
AAAACATTCA AGACGAGAAA AGCATCTGCA CAGGCCAGCC TAGCATCTAA AGATAAAACT 
CCTAAATCAA AGAGTAAGAA AAGGCATTCT GCTCAACTAA AAAGCAGAGT TAAAAATACT 
GAAACTGGGA GTGATTTTTC CATGTTTGAA GCTTTGCGGG ATACTATTTA TTCTGAAGTA 
GCTACATTAA TTTCTCAAAA TGAATCTCAT CCACATTTTC TTATTGAACT CTTCCATGAA 
CTTCAGCTAC TTAATACAGA CTACTTGAGA CAAAGGGCTT TATATGCATT GCAGGACATA 
GTAACCAGAC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATGTAAA GTCAGTGAAT 
TCTGGTACTT GGATAGCATC AAACTCAGAG CTTACCCCCA GTGAAAGCCT TGCTACCACT 
GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT 
GATGCTGATA ATGCTAGCGT CATGTCTGTA TCTTCAAATT TTGAGCCTTT TGCAACAGAT 
GATCTAGGTA ACACTGTGAT TCACTTAGAT CAGGCATTAG CCAGAATGAG GGAATACGAG 
CGTATGAAGA CTGAGGCTGA AAGTAGCACA AATATAAGAT GCACCTGCAG AATTCTTGAG 
GATGAGGATG GTGCTGCCGC CACAAGTATG GTTACTAATT TAGAAGAAAC TCCTATTGAA 
AATCATGGTT CACAACAACC TGTAAGTGAA GTTTCTACTG TTCCATGTCC TAGAATTGAT 
ACTCAGCAGC TGGACCGGCA AATTAAAGCC ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG CTCTTCTCAA CTTCTAACTT CAGTAAGGCG TATGGTTTTG 
ACCCTCACCC AACAAAATGA TGAGAGCAAA GAATTTGTGA AGTTCTTTCA TAAACAACTT 
GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA AGACTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTGTTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTATAACT GTTAAACAGA AGTGCAAAAG GAAAATAGAA 
GCAGCTGGAG TGATACAGTC TTATGCCAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAT 
GGCTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAAAC TGAAACAGTT 
AAGCCGACTC AGACATCTGA GATATATGAT GGTGATGGTC CCAAAAATGT GAGATCAGAT 
GTTTCTGATC AAGAGGAAGA TGAAGAAAGT GAAGAATGTC CGGTGTCTAT TAACTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAT GGAAGTGGAG AAGATGAGAA TGAGGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTG GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAGCTACAG AAGAAACTGA ACACGATGAT CAGGTACTAC AACATGACTT TGAAAAATCA 
GGAGAAAGCA AAAATGTCCC ATCAGAACAA GATCCCACTA CTAGTAAAGG CAAGAAATAT 
GACCAAGATA GTACTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAAA TGAGCAGCCT 
TTGAATAGCG CTGTCCAAAA AGACTCTCTG ACTACCATCG ATTCATCTAA ACAGCCTAAC 
CCTTTGCCTT TACCTTTACC TGAAATCGAA ACCTTGGTGC CTACAGTCAA AGAAGTTAAA 
TCTGCTCAAG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AACATAAGTC AAAAATCTGA TGAAGAAGAC 
TTTGTGAAAG TTGAAGATTT ACCACTTAAA CTGACAATAT ACTCAGAGGC AGATCTAAGG 
AAGAAAATGG TAGAAGAGCA AGAGAAAAAC CATTTATCTG GTGAAATACT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAAAC AGTGGGAGCC 
CAAAGCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259872.1
CDS	426..6497
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLDCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404164.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404164.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAGCATGAC 
TTATTAAAAA GGATGTTACA ACAGCAGGAG CAACTGAGAG CTCTGCAGGG AAGACAGGCT 
GCTCTTCTTG CTCTGCAGCA CAAAGCAGAG CAAGCTATTG CTGTGATGGA TGATTCTGTT 
GTAACAGAAA CTACAGGTAG CGTATCTGGA GTTAGTATCA CCTCTGAACT AAATGAAGAA 
TTGAATGATT TAATTCAGCG TTTTCATAAT CAGCTTCGTG ATTCTCAGCC TCCAACTGTT 
CCAGACAATA GAAGACAAGC AGAAAGTCTT TCATTAACTA GGGAGGTATC CCAGAGCAGG 
AATCCATCAG TTTCAGAACA CTTACCTGAT GAGAAAGTAC AACTGTTTAG CAAAATGAGA 
GTATTACAGG AAAAGAAACA AAAAATGGAC AAACTACTTG GAGAACTTCA TACACTTCGA 
GATCAACATC TGAACAATTC ATCATTTGTG CCTTCTTCAG CTTCTCCACA AAGAAGTGTG 
GATCAGAGAA GTACAACTTC AGCACCCTCT GCTCCTATAG GCTTAGCACC AGTTGTCAAT 
GGAGAATCCA ACAGCTTCAC ATCATCTGTT CCTTATCCTG TTGCTTCTCT AGTTTCTCAG 
AATGAGAGTG AAAACGAAGG ACATCTAAAT CCAACTGAAA AACTCCAGAA GTTAAATGAA 
GTTCGAAAGA GATTGAATGA ACTAAGAGAA CTAGTTCATT ATTACGAACA AACATCAGAT 
ATGATGACAG ATGCTGTAAA TGAAAACACA AAAGATGAAG AAACTGAAGA GTCAGAATAT 
GATTCTGAAC ATGAAAACCC TGAACCTGTT ACTAACATTC GAAATCCTCA AGTAGCTGCC 
ACTTGGAATG AAGTAAATAG TAATTCTAAT GCACAGTGTG TTTCTAATAA TAGAGAGGGG 
AGATCAGTTA ATTCTAATTG TGAAATTAAC AACAGATCTG CTGCCAACAT AAGGACTCTG 
AACATGCCTC CTTCTTTAGA TTGTCACTAT AATAGAGAAG GAGAACAGGG GATTCATGGT 
GCACAAGGTG AAGACGATGA GGAAGAAGAA GAAGCAGAAG ATGAAGGAGT CAGTGGGGCT 
TCATTAACTA GTCATAGGAG CAGTCTGGTT GATGAGGCTG CAGAAGACGC TGAATTTGAA 
CAGAAGATCA ATAGACTTAT GGCTGCCAAA CAGAAATTAA GACAGTTACA AGATCTTGTT 
GCTATGGTAC AGGATGATGA TGCAGCAGAT CATGGAGTTA TCTCTGCCAA TACTTCAAAT 
TTGGATGATT TTTACCCAGC AGAAGAAGAC AACAAACAAA GTGCAAATAA TACTAGAGGA 
AATGCCAATA AAACACAGAA AGATGCTGGA ATAAATGAGA AGGCAAGAGA GAAGTTTTAT 
GAGGCTAAAC TACAGCAGCA ACAGAGAGAG CTCAGACAAT TGCAGGAAGA AAGAAAGAAA 
CTGATTGAGA TTCAAGAGAA AATTCAAGCA TTACAGAAGG CATGCCCTGA CTTACAGCTG 
TCAGCCACTA GTGCGGGTAA TTGTCCCACA AAAAAATATA TCCCAGCTGT TACTTCAACC 
CCAGTTGTTA ATGGGAATGA AACCAGTACA AGCAAATCTG CTTTTGAGCC TGCAGATCCT 
TCAGGAGTGG ATAATGAGCT GTGGTCAGAA ATGCGAAGAC ATGAGATGTT AAGGGAAGAG 
CTACGGCAGA GAAGAAAGCA GCTTGAAGCA CTGATGGCTG AACATCAGAG GAGGCAAGGT 
CTAGCTGAAA CTACATCCCC TCTGGCTGTG TCATTGAGAA GTGATGGATC TGAGAACCTG 
TGTACGCCTC AACAGAGTAG AACAGAAAAA ACCATGGCAA CATGGGGAGG TTCTACCCAG 
TGTGCACTAG ATGAAGAAGG AGATGAAGAT GGTTACCTTT CTGAAGGAGT TGTTCGGACA 
GATGAAGAGG AGGAAGAAGA AGAGCAAGAT GCAAGTTCGA ATGATAACTT TTCTATGTAT 
CCTCCTAACA GTGCGAATCA TAACTCTTAC AACATAAAAG AAACTAAGAA TAGGTGGAAG 
AATAGTCGCC CTTTTACAGC AGATGGAAAT TATCGTCCTT TAGCCAAGAC AAGACAGCAG 
AATATCAGCA TGCAACGTCA GGAAAACCTT CGTTGGATGT CCGAACTCTC TTACGTAGAG 
GAGAAAGAAC AATGGCAAGA ACAAATTAAT CAGCTGAAGA AACAACTTGA TTTCAGTGTC 
AATATTTGTC AGACTTTGAT GCAAGACCAG CAGACGCTGT CTTGTCTGCT GCAAACTCTT 
CTCACAGGTC CTTACAGTGT TATGCCCAGC AATGTAGCTT CTCCTCAAGT ACACCTTATA 
ATGCACCAGT TGAATCAGTG CTATACTCAG CTAACATGGC AACAGAATAA TGTGCAAAGG 
TTGAAACAAA TGCTAAATGA ACTTATGCGC CAACAGAATC AGCACCCAGA AAAACCTGGA 
AGCCAGGAAA GAGGCAGTAG TGCACCACAG CCTTCTTCTC CCAGTTTATT TTGTCCTTTC 
AGCTTTCCAT CACAGCCTGT AAATCTATTT AACCTACCCG GGTTTACTAA TTTTTCATCA 
TTTGCACCAG GTATGAATTT TAGCCCTTTA TTTCCTTCTA ATTTTGGAGA ATTTTCTCAG 
AATATTTCTA CACCTACTGA ACAGCAGCAA CCATTAGCCC AGAATTCTTC AGGGAAAACA 
GAATATATGG CTTTTCCAAA ACCTTTTGAA AGCAGTTCTT CTATTGGAGC AGAAAAACAA 
AGGAATCAAA AACAGCCTGG AGAGGAGGTG GAAAACAGTA GGACAGCGTG GTTATATGAC 
CAAGAAGGTG AAGTAGAAAA ACCATTTATC AAGACTGGAT TTCCAGTGTC TGTAGAGAAA 
ACTACAAATA GTAATCGCAA AAATCAATTA GATACCGGCC GAAGAAGACG CCAGTTTGAT 
GAAGAGTCAT TAGAAAGCTT TAGCAGTATG CCTGATCCAG TAGATCCGAC AACAGTAACC 
AAAACATTCA AGACGAGAAA AGCATCTGCA CAGGCCAGCC TAGCATCTAA AGATAAAACT 
CCTAAATCAA AGAGTAAGAA AAGGCATTCT GCTCAACTAA AAAGCAGAGT TAAAAATACT 
GAAACTGGGA GTGATTTTTC CATGTTTGAA GCTTTGCGGG ATACTATTTA TTCTGAAGTA 
GCTACATTAA TTTCTCAAAA TGAATCTCAT CCACATTTTC TTATTGAACT CTTCCATGAA 
CTTCAGCTAC TTAATACAGA CTACTTGAGA CAAAGGGCTT TATATGCATT GCAGGACATA 
GTAACCAGAC ATATTTCTGA GAACCATGAA AAAGAAGGAG AAAATGTAAA GTCAGTGAAT 
TCTGGTACTT GGATAGCATC AAACTCAGAG CTTACCCCCA GTGAAAGCCT TGCTACCACT 
GATGATGAAA CTTTTGAGAA GAACTTTGAG AGAGAAACAC ATAAAATAAG TGAGCAAAAT 
GATGCTGATA ATGCTAGCGT CATGTCTGTA TCTTCAAATT TTGAGCCTTT TGCAACAGAT 
GATCTAGGTA ACACTGTGAT TCACTTAGAT CAGGCATTAG CCAGAATGAG GGAATACGAG 
CGTATGAAGA CTGAGGCTGA AAGTAGCACA AATATAAGAT GCACCTGCAG AATTCTTGAG 
GATGAGGATG GTGCTGCCGC CACAAGTATG GTTACTAATT TAGAAGAAAC TCCTATTGAA 
AATCATGGTT CACAACAACC TGTAAGTGAA GTTTCTACTG TTCCATGTCC TAGAATTGAT 
ACTCAGCAGC TGGACCGGCA AATTAAAGCC ATTATGAAAG AAGTCATTCC TTTTTTGAAG 
GAGCACATGG ATGAAGTATG CTCTTCTCAA CTTCTAACTT CAGTAAGGCG TATGGTTTTG 
ACCCTCACCC AACAAAATGA TGAGAGCAAA GAATTTGTGA AGTTCTTTCA TAAACAACTT 
GGAAGTATAT TACAGGATTC ACTGGCAAAA TTTGCTGGCA GAAAACTGAA AGACTGTGGA 
GAAGATCTTC TTGTAGAGAT ATCTGAAGTG TTGTTTAATG AATTGGCTTT CTTTAAGCTC 
ATGCAAGATT TGGATAATAA TAGTATAACT GTTAAACAGA AGTGCAAAAG GAAAATAGAA 
GCAGCTGGAG TGATACAGTC TTATGCCAAA GAGGCCAAAA GGATTCTTGA AGGAGATCAT 
GGCTCACCTG CTGGAGAAAT TGATGATGAA GACAAAGACA AGGATGAAAC TGAAACAGTT 
AAGCCGACTC AGACATCTGA GATATATGAT GGTGATGGTC CCAAAAATGT GAGATCAGAT 
GTTTCTGATC AAGAGGAAGA TGAAGAAAGT GAAGAATGTC CGGTGTCTAT TAACTTGTCC 
AAAGCTGAAA CTCAGGCTTT GACTAATTAT GGAAGTGGAG AAGATGAGAA TGAGGATGAA 
GAAATAGAAG AATTTGAAGA GGGACCTGTG GATGTCCAGA CGTCACTTCA GGCTAACACT 
GAAGCTACAG AAGAAACTGA ACACGATGAT CAGGTACTAC AACATGACTT TGAAAAATCA 
GGAGAAAGCA AAAATGTCCC ATCAGAACAA GATCCCACTA CTAGTAAAGG CAAGAAATAT 
GACCAAGATA GTACTCCTGT GAAACCCTGT TACCTCAATA TCTTGGAAAA TGAGCAGCCT 
TTGAATAGCG CTGTCCAAAA AGACTCTCTG ACTACCATCG ATTCATCTAA ACAGCCTAAC 
CCTTTGCCTT TACCTTTACC TGAAATCGAA ACCTTGGTGC CTACAGTCAA AGAAGTTAAA 
TCTGCTCAAG AAACTCCTGA AAGCTCTCTG GCTGGAAGTC CTGATACTGA ATCTCCAGTG 
TTAGTGAATG ACTATGAAGC AGAATCTGGT AACATAAGTC AAAAATCTGA TGAAGAAGAC 
TTTGTGAAAG TTGAAGATTT ACCACTTAAA CTGACAATAT ACTCAGAGGC AGATCTAAGG 
AAGAAAATGG TAGAAGAGCA AGAGAAAAAC CATTTATCTG GTGAAATACT ATGTGAAATG 
CAGACCGAAG AATTAGCTGG GAATTCTCAG ACACTAAAAG AACCTGAAAC AGTGGGAGCC 
CAAAGCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404165.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259873.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X25, mRNA.

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CloneID	OCe294058
Clone ID Related Accession (Same CDS sequence)	XM_022404165.1
Accession Version	XM_022404165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGCAGATTGT CACTATAATA GAGAAGGAGA ACAGGGGATT 
CATGGTGCAC AAGGTGAAGA CGATGAGGAA GAAGAAGAAG CAGAAGATGA AGGAGTCAGT 
GGGGCTTCAT TAACTAGTCA TAGGAGCAGT CTGGTTGATG AGGCTGCAGA AGACGCTGAA 
TTTGAACAGA AGATCAATAG ACTTATGGCT GCCAAACAGA AATTAAGACA GTTACAAGAT 
CTTGTTGCTA TGGTACAGGA TGATGATGCA GCAGATCATG GAGTTATCTC TGCCAATACT 
TCAAATTTGG ATGATTTTTA CCCAGCAGAA GAAGACAACA AACAAAGTGC AAATAATACT 
AGAGGAAATG CCAATAAAAC ACAGAAAGAT GCTGGAATAA ATGAGAAGGC AAGAGAGAAG 
TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAGCTCA GACAATTGCA GGAAGAAAGA 
AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGCATTAC AGAAGGCATG CCCTGACTTA 
CAGCTGTCAG CCACTAGTGC GGGTAATTGT CCCACAAAAA AATATATCCC AGCTGTTACT 
TCAACCCCAG TTGTTAATGG GAATGAAACC AGTACAAGCA AATCTGCTTT TGAGCCTGCA 
GATCCTTCAG GAGTGGATAA TGAGCTGTGG TCAGAAATGC GAAGACATGA GATGTTAAGG 
GAAGAGCTAC GGCAGAGAAG AAAGCAGCTT GAAGCACTGA TGGCTGAACA TCAGAGGAGG 
CAAGGTCTAG CTGAAACTAC ATCCCCTCTG GCTGTGTCAT TGAGAAGTGA TGGATCTGAG 
AACCTGTGTA CGCCTCAACA GAGTAGAACA GAAAAAACCA TGGCAACATG GGGAGGTTCT 
ACCCAGTGTG CACTAGATGA AGAAGGAGAT GAAGATGGTT ACCTTTCTGA AGGAGTTGTT 
CGGACAGATG AAGAGGAGGA AGAAGAAGAG CAAGATGCAA GTTCGAATGA TAACTTTTCT 
ATGTATCCTC CTAACAGTGC GAATCATAAC TCTTACAACA TAAAAGAAAC TAAGAATAGG 
TGGAAGAATA GTCGCCCTTT TACAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA 
CAGCAGAATA TCAGCATGCA ACGTCAGGAA AACCTTCGTT GGATGTCCGA ACTCTCTTAC 
GTAGAGGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TGAAGAAACA ACTTGATTTC 
AGTGTCAATA TTTGTCAGAC TTTGATGCAA GACCAGCAGA CGCTGTCTTG TCTGCTGCAA 
ACTCTTCTCA CAGGTCCTTA CAGTGTTATG CCCAGCAATG TAGCTTCTCC TCAAGTACAC 
CTTATAATGC ACCAGTTGAA TCAGTGCTAT ACTCAGCTAA CATGGCAACA GAATAATGTG 
CAAAGGTTGA AACAAATGCT AAATGAACTT ATGCGCCAAC AGAATCAGCA CCCAGAAAAA 
CCTGGAAGCC AGGAAAGAGG CAGTAGTGCA CCACAGCCTT CTTCTCCCAG TTTATTTTGT 
CCTTTCAGCT TTCCATCACA GCCTGTAAAT CTATTTAACC TACCCGGGTT TACTAATTTT 
TCATCATTTG CACCAGGTAT GAATTTTAGC CCTTTATTTC CTTCTAATTT TGGAGAATTT 
TCTCAGAATA TTTCTACACC TACTGAACAG CAGCAACCAT TAGCCCAGAA TTCTTCAGGG 
AAAACAGAAT ATATGGCTTT TCCAAAACCT TTTGAAAGCA GTTCTTCTAT TGGAGCAGAA 
AAACAAAGGA ATCAAAAACA GCCTGGAGAG GAGGTGGAAA ACAGTAGGAC AGCGTGGTTA 
TATGACCAAG AAGGTGAAGT AGAAAAACCA TTTATCAAGA CTGGATTTCC AGTGTCTGTA 
GAGAAAACTA CAAATAGTAA TCGCAAAAAT CAATTAGATA CCGGCCGAAG AAGACGCCAG 
TTTGATGAAG AGTCATTAGA AAGCTTTAGC AGTATGCCTG ATCCAGTAGA TCCGACAACA 
GTAACCAAAA CATTCAAGAC GAGAAAAGCA TCTGCACAGG CCAGCCTAGC ATCTAAAGAT 
AAAACTCCTA AATCAAAGAG TAAGAAAAGG CATTCTGCTC AACTAAAAAG CAGAGTTAAA 
AATACTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGGAAC 
AGACATTCAG CCCAGACTGA AGAGCCTGTT CAAGCAAAAG TATTCAGCAG AAAGAATCTT 
GAGCAGCTGG AAAAAATAAT AAAATATAGT AGGTCTACAG AGATATCTTC AGAAACTGGG 
AGTGATTTTT CCATGTTTGA AGCTTTGCGG GATACTATTT ATTCTGAAGT AGCTACATTA 
ATTTCTCAAA ATGAATCTCA TCCACATTTT CTTATTGAAC TCTTCCATGA ACTTCAGCTA 
CTTAATACAG ACTACTTGAG ACAAAGGGCT TTATATGCAT TGCAGGACAT AGTAACCAGA 
CATATTTCTG AGAACCATGA AAAAGAAGGA GAAAATGTAA AGTCAGTGAA TTCTGGTACT 
TGGATAGCAT CAAACTCAGA GCTTACCCCC AGTGAAAGCC TTGCTACCAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA GAGAGAAACA CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGCG TCATGTCTGT ATCTTCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GGGAATACGA GCGTATGAAG 
ACTGAGGCTG AAAGTAGCAC AAATATAAGA TGCACCTGCA GAATTCTTGA GGATGAGGAT 
GGTGCTGCCG CCACAAGTAT GGTTACTAAT TTAGAAGAAA CTCCTATTGA AAATCATGGT 
TCACAACAAC CTGTAAGTGA AGTTTCTACT GTTCCATGTC CTAGAATTGA TACTCAGCAG 
CTGGACCGGC AAATTAAAGC CATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG 
GATGAAGTAT GCTCTTCTCA ACTTCTAACT TCAGTAAGGC GTATGGTTTT GACCCTCACC 
CAACAAAATG ATGAGAGCAA AGAATTTGTG AAGTTCTTTC ATAAACAACT TGGAAGTATA 
TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AAGACTGTGG AGAAGATCTT 
CTTGTAGAGA TATCTGAAGT GTTGTTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT 
TTGGATAATA ATAGTATAAC TGTTAAACAG AAGTGCAAAA GGAAAATAGA AGCAGCTGGA 
GTGATACAGT CTTATGCCAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA TGGCTCACCT 
GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAAA CTGAAACAGT TAAGCCGACT 
CAGACATCTG AGATATATGA TGGTGATGGT CCCAAAAATG TGAGATCAGA TGTTTCTGAT 
CAAGAGGAAG ATGAAGAAAG TGAAGAATGT CCGGTGTCTA TTAACTTGTC CAAAGCTGAA 
ACTCAGGCTT TGACTAATTA TGGAAGTGGA GAAGATGAGA ATGAGGATGA AGAAATAGAA 
GAATTTGAAG AGGGACCTGT GGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAGCTACA 
GAAGAAACTG AACACGATGA TCAGGTACTA CAACATGACT TTGAAAAATC AGGAGAAAGC 
AAAAATGTCC CATCAGAACA AGATCCCACT ACTAGTAAAG GCAAGAAATA TGACCAAGAT 
AGTACTCCTG TGAAACCCTG TTACCTCAAT ATCTTGGAAA ATGAGCAGCC TTTGAATAGC 
GCTGTCCAAA AAGACTCTCT GACTACCATC GATTCATCTA AACAGCCTAA CCCTTTGCCT 
TTACCTTTAC CTGAAATCGA AACCTTGGTG CCTACAGTCA AAGAAGTTAA ATCTGCTCAA 
GAAACTCCTG AAAGCTCTCT GGCTGGAAGT CCTGATACTG AATCTCCAGT GTTAGTGAAT 
GACTATGAAG CAGAATCTGG TAACATAAGT CAAAAATCTG ATGAAGAAGA CTTTGTGAAA 
GTTGAAGATT TACCACTTAA ACTGACAATA TACTCAGAGG CAGATCTAAG GAAGAAAATG 
GTAGAAGAGC AAGAGAAAAA CCATTTATCT GGTGAAATAC TATGTGAAAT GCAGACCGAA 
GAATTAGCTG GGAATTCTCA GACACTAAAA GAACCTGAAA CAGTGGGAGC CCAAAGCGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259873.1
CDS	426..6548
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404165.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404165.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGCAGATTGT CACTATAATA GAGAAGGAGA ACAGGGGATT 
CATGGTGCAC AAGGTGAAGA CGATGAGGAA GAAGAAGAAG CAGAAGATGA AGGAGTCAGT 
GGGGCTTCAT TAACTAGTCA TAGGAGCAGT CTGGTTGATG AGGCTGCAGA AGACGCTGAA 
TTTGAACAGA AGATCAATAG ACTTATGGCT GCCAAACAGA AATTAAGACA GTTACAAGAT 
CTTGTTGCTA TGGTACAGGA TGATGATGCA GCAGATCATG GAGTTATCTC TGCCAATACT 
TCAAATTTGG ATGATTTTTA CCCAGCAGAA GAAGACAACA AACAAAGTGC AAATAATACT 
AGAGGAAATG CCAATAAAAC ACAGAAAGAT GCTGGAATAA ATGAGAAGGC AAGAGAGAAG 
TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAGCTCA GACAATTGCA GGAAGAAAGA 
AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGCATTAC AGAAGGCATG CCCTGACTTA 
CAGCTGTCAG CCACTAGTGC GGGTAATTGT CCCACAAAAA AATATATCCC AGCTGTTACT 
TCAACCCCAG TTGTTAATGG GAATGAAACC AGTACAAGCA AATCTGCTTT TGAGCCTGCA 
GATCCTTCAG GAGTGGATAA TGAGCTGTGG TCAGAAATGC GAAGACATGA GATGTTAAGG 
GAAGAGCTAC GGCAGAGAAG AAAGCAGCTT GAAGCACTGA TGGCTGAACA TCAGAGGAGG 
CAAGGTCTAG CTGAAACTAC ATCCCCTCTG GCTGTGTCAT TGAGAAGTGA TGGATCTGAG 
AACCTGTGTA CGCCTCAACA GAGTAGAACA GAAAAAACCA TGGCAACATG GGGAGGTTCT 
ACCCAGTGTG CACTAGATGA AGAAGGAGAT GAAGATGGTT ACCTTTCTGA AGGAGTTGTT 
CGGACAGATG AAGAGGAGGA AGAAGAAGAG CAAGATGCAA GTTCGAATGA TAACTTTTCT 
ATGTATCCTC CTAACAGTGC GAATCATAAC TCTTACAACA TAAAAGAAAC TAAGAATAGG 
TGGAAGAATA GTCGCCCTTT TACAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA 
CAGCAGAATA TCAGCATGCA ACGTCAGGAA AACCTTCGTT GGATGTCCGA ACTCTCTTAC 
GTAGAGGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TGAAGAAACA ACTTGATTTC 
AGTGTCAATA TTTGTCAGAC TTTGATGCAA GACCAGCAGA CGCTGTCTTG TCTGCTGCAA 
ACTCTTCTCA CAGGTCCTTA CAGTGTTATG CCCAGCAATG TAGCTTCTCC TCAAGTACAC 
CTTATAATGC ACCAGTTGAA TCAGTGCTAT ACTCAGCTAA CATGGCAACA GAATAATGTG 
CAAAGGTTGA AACAAATGCT AAATGAACTT ATGCGCCAAC AGAATCAGCA CCCAGAAAAA 
CCTGGAAGCC AGGAAAGAGG CAGTAGTGCA CCACAGCCTT CTTCTCCCAG TTTATTTTGT 
CCTTTCAGCT TTCCATCACA GCCTGTAAAT CTATTTAACC TACCCGGGTT TACTAATTTT 
TCATCATTTG CACCAGGTAT GAATTTTAGC CCTTTATTTC CTTCTAATTT TGGAGAATTT 
TCTCAGAATA TTTCTACACC TACTGAACAG CAGCAACCAT TAGCCCAGAA TTCTTCAGGG 
AAAACAGAAT ATATGGCTTT TCCAAAACCT TTTGAAAGCA GTTCTTCTAT TGGAGCAGAA 
AAACAAAGGA ATCAAAAACA GCCTGGAGAG GAGGTGGAAA ACAGTAGGAC AGCGTGGTTA 
TATGACCAAG AAGGTGAAGT AGAAAAACCA TTTATCAAGA CTGGATTTCC AGTGTCTGTA 
GAGAAAACTA CAAATAGTAA TCGCAAAAAT CAATTAGATA CCGGCCGAAG AAGACGCCAG 
TTTGATGAAG AGTCATTAGA AAGCTTTAGC AGTATGCCTG ATCCAGTAGA TCCGACAACA 
GTAACCAAAA CATTCAAGAC GAGAAAAGCA TCTGCACAGG CCAGCCTAGC ATCTAAAGAT 
AAAACTCCTA AATCAAAGAG TAAGAAAAGG CATTCTGCTC AACTAAAAAG CAGAGTTAAA 
AATACTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGGAAC 
AGACATTCAG CCCAGACTGA AGAGCCTGTT CAAGCAAAAG TATTCAGCAG AAAGAATCTT 
GAGCAGCTGG AAAAAATAAT AAAATATAGT AGGTCTACAG AGATATCTTC AGAAACTGGG 
AGTGATTTTT CCATGTTTGA AGCTTTGCGG GATACTATTT ATTCTGAAGT AGCTACATTA 
ATTTCTCAAA ATGAATCTCA TCCACATTTT CTTATTGAAC TCTTCCATGA ACTTCAGCTA 
CTTAATACAG ACTACTTGAG ACAAAGGGCT TTATATGCAT TGCAGGACAT AGTAACCAGA 
CATATTTCTG AGAACCATGA AAAAGAAGGA GAAAATGTAA AGTCAGTGAA TTCTGGTACT 
TGGATAGCAT CAAACTCAGA GCTTACCCCC AGTGAAAGCC TTGCTACCAC TGATGATGAA 
ACTTTTGAGA AGAACTTTGA GAGAGAAACA CATAAAATAA GTGAGCAAAA TGATGCTGAT 
AATGCTAGCG TCATGTCTGT ATCTTCAAAT TTTGAGCCTT TTGCAACAGA TGATCTAGGT 
AACACTGTGA TTCACTTAGA TCAGGCATTA GCCAGAATGA GGGAATACGA GCGTATGAAG 
ACTGAGGCTG AAAGTAGCAC AAATATAAGA TGCACCTGCA GAATTCTTGA GGATGAGGAT 
GGTGCTGCCG CCACAAGTAT GGTTACTAAT TTAGAAGAAA CTCCTATTGA AAATCATGGT 
TCACAACAAC CTGTAAGTGA AGTTTCTACT GTTCCATGTC CTAGAATTGA TACTCAGCAG 
CTGGACCGGC AAATTAAAGC CATTATGAAA GAAGTCATTC CTTTTTTGAA GGAGCACATG 
GATGAAGTAT GCTCTTCTCA ACTTCTAACT TCAGTAAGGC GTATGGTTTT GACCCTCACC 
CAACAAAATG ATGAGAGCAA AGAATTTGTG AAGTTCTTTC ATAAACAACT TGGAAGTATA 
TTACAGGATT CACTGGCAAA ATTTGCTGGC AGAAAACTGA AAGACTGTGG AGAAGATCTT 
CTTGTAGAGA TATCTGAAGT GTTGTTTAAT GAATTGGCTT TCTTTAAGCT CATGCAAGAT 
TTGGATAATA ATAGTATAAC TGTTAAACAG AAGTGCAAAA GGAAAATAGA AGCAGCTGGA 
GTGATACAGT CTTATGCCAA AGAGGCCAAA AGGATTCTTG AAGGAGATCA TGGCTCACCT 
GCTGGAGAAA TTGATGATGA AGACAAAGAC AAGGATGAAA CTGAAACAGT TAAGCCGACT 
CAGACATCTG AGATATATGA TGGTGATGGT CCCAAAAATG TGAGATCAGA TGTTTCTGAT 
CAAGAGGAAG ATGAAGAAAG TGAAGAATGT CCGGTGTCTA TTAACTTGTC CAAAGCTGAA 
ACTCAGGCTT TGACTAATTA TGGAAGTGGA GAAGATGAGA ATGAGGATGA AGAAATAGAA 
GAATTTGAAG AGGGACCTGT GGATGTCCAG ACGTCACTTC AGGCTAACAC TGAAGCTACA 
GAAGAAACTG AACACGATGA TCAGGTACTA CAACATGACT TTGAAAAATC AGGAGAAAGC 
AAAAATGTCC CATCAGAACA AGATCCCACT ACTAGTAAAG GCAAGAAATA TGACCAAGAT 
AGTACTCCTG TGAAACCCTG TTACCTCAAT ATCTTGGAAA ATGAGCAGCC TTTGAATAGC 
GCTGTCCAAA AAGACTCTCT GACTACCATC GATTCATCTA AACAGCCTAA CCCTTTGCCT 
TTACCTTTAC CTGAAATCGA AACCTTGGTG CCTACAGTCA AAGAAGTTAA ATCTGCTCAA 
GAAACTCCTG AAAGCTCTCT GGCTGGAAGT CCTGATACTG AATCTCCAGT GTTAGTGAAT 
GACTATGAAG CAGAATCTGG TAACATAAGT CAAAAATCTG ATGAAGAAGA CTTTGTGAAA 
GTTGAAGATT TACCACTTAA ACTGACAATA TACTCAGAGG CAGATCTAAG GAAGAAAATG 
GTAGAAGAGC AAGAGAAAAA CCATTTATCT GGTGAAATAC TATGTGAAAT GCAGACCGAA 
GAATTAGCTG GGAATTCTCA GACACTAAAA GAACCTGAAA CAGTGGGAGC CCAAAGCGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_014120141.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975616.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X8, mRNA.

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CloneID	OCe40623
Clone ID Related Accession (Same CDS sequence)	XM_014120141.1 , XM_022404146.1
Accession Version	XM_014120141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGAT 
TGTCACTATA ATAGAGAAGG AGAACAGGGG ATTCATGGTG CACAAGGTGA AGACGATGAG 
GAAGAAGAAG AAGCAGAAGA TGAAGGAGTC AGTGGGGCTT CATTAACTAG TCATAGGAGC 
AGTCTGGTTG ATGAGGCTGC AGAAGACGCT GAATTTGAAC AGAAGATCAA TAGACTTATG 
GCTGCCAAAC AGAAATTAAG ACAGTTACAA GATCTTGTTG CTATGGTACA GGATGATGAT 
GCAGCAGATC ATGGAGTTAT CTCTGCCAAT ACTTCAAATT TGGATGATTT TTACCCAGCA 
GAAGAAGACA ACAAACAAAG TGCAAATAAT ACTAGAGGAA ATGCCAATAA AACACAGAAA 
GATGCTGGAA TAAATGAGAA GGCAAGAGAG AAGTTTTATG AGGCTAAACT ACAGCAGCAA 
CAGAGAGAGC TCAGACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA 
ATTCAAGCAT TACAGAAGGC ATGCCCTGAC TTACAGCTGT CAGCCACTAG TGCGGGTAAT 
TGTCCCACAA AAAAATATAT CCCAGCTGTT ACTTCAACCC CAGTTGTTAA TGGGAATGAA 
ACCAGTACAA GCAAATCTGC TTTTGAGCCT GCAGATCCTT CAGGAGTGGA TAATGAGCTG 
TGGTCAGAAA TGCGAAGACA TGAGATGTTA AGGGAAGAGC TACGGCAGAG AAGAAAGCAG 
CTTGAAGCAC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TAGCTGAAAC TACATCCCCT 
CTGGCTGTGT CATTGAGAAG TGATGGATCT GAGAACCTGT GTACGCCTCA ACAGAGTAGA 
ACAGAAAAAA CCATGGCAAC ATGGGGAGGT TCTACCCAGT GTGCACTAGA TGAAGAAGGA 
GATGAAGATG GTTACCTTTC TGAAGGAGTT GTTCGGACAG ATGAAGAGGA GGAAGAAGAA 
GAGCAAGATG CAAGTTCGAA TGATAACTTT TCTATGTATC CTCCTAACAG TGCGAATCAT 
AACTCTTACA ACATAAAAGA AACTAAGAAT AGGTGGAAGA ATAGTCGCCC TTTTACAGCA 
GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAGCAGA ATATCAGCAT GCAACGTCAG 
GAAAACCTTC GTTGGATGTC CGAACTCTCT TACGTAGAGG AGAAAGAACA ATGGCAAGAA 
CAAATTAATC AGCTGAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTGATG 
CAAGACCAGC AGACGCTGTC TTGTCTGCTG CAAACTCTTC TCACAGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTAGCTTC TCCTCAAGTA CACCTTATAA TGCACCAGTT GAATCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTGCAAAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AACAGAATCA GCACCCAGAA AAACCTGGAA GCCAGGAAAG AGGCAGTAGT 
GCACCACAGC CTTCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCATC ACAGCCTGTA 
AATCTATTTA ACCTACCCGG GTTTACTAAT TTTTCATCAT TTGCACCAGG TATGAATTTT 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAA TTTTCTCAGA ATATTTCTAC ACCTACTGAA 
CAGCAGCAAC CATTAGCCCA GAATTCTTCA GGGAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGGA 
GAGGAGGTGG AAAACAGTAG GACAGCGTGG TTATATGACC AAGAAGGTGA AGTAGAAAAA 
CCATTTATCA AGACTGGATT TCCAGTGTCT GTAGAGAAAA CTACAAATAG TAATCGCAAA 
AATCAATTAG ATACCGGCCG AAGAAGACGC CAGTTTGATG AAGAGTCATT AGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCGACA ACAGTAACCA AAACATTCAA GACGAGAAAA 
GCATCTGCAC AGGCCAGCCT AGCATCTAAA GATAAAACTC CTAAATCAAA GAGTAAGAAA 
AGGCATTCTG CTCAACTAAA AAGCAGAGTT AAAAATACTG GGTATGAAAG TGCCAGTGTG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CTTGAGCAGC TGGAAAAAAT AATAAAATAT 
AGTAGGTCTA CAGAGATATC TTCAGCACAT GCTAGGAGAA TACTACAGCA GTCTAACAGA 
AATGCATGCA ATGAAGCACC AGAAACTGGG AGTGATTTTT CCATGTTTGA AGCTTTGCGG 
GATACTATTT ATTCTGAAGT AGCTACATTA ATTTCTCAAA ATGAATCTCA TCCACATTTT 
CTTATTGAAC TCTTCCATGA ACTTCAGCTA CTTAATACAG ACTACTTGAG ACAAAGGGCT 
TTATATGCAT TGCAGGACAT AGTAACCAGA CATATTTCTG AGAACCATGA AAAAGAAGGA 
GAAAATGTAA AGTCAGTGAA TTCTGGTACT TGGATAGCAT CAAACTCAGA GCTTACCCCC 
AGTGAAAGCC TTGCTACCAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGCG TCATGTCTGT ATCTTCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACTGTGA TTCACTTAGA TCAGGCATTA 
GCCAGAATGA GGGAATACGA GCGTATGAAG ACTGAGGCTG AAAGTAGCAC AAATATAAGA 
TGCACCTGCA GAATTCTTGA GGATGAGGAT GGTGCTGCCG CCACAAGTAT GGTTACTAAT 
TTAGAAGAAA CTCCTATTGA AAATCATGGT TCACAACAAC CTGTAAGTGA AGTTTCTACT 
GTTCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC CATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCTTCTCA ACTTCTAACT 
TCAGTAAGGC GTATGGTTTT GACCCTCACC CAACAAAATG ATGAGAGCAA AGAATTTGTG 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AAGTGCAAAA GGAAAATAGA AGCAGCTGGA GTGATACAGT CTTATGCCAA AGAGGCCAAA 
AGGATTCTTG AAGGAGATCA TGGCTCACCT GCTGGAGAAA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCCGACT CAGACATCTG AGATATATGA TGGTGATGGT 
CCCAAAAATG TGAGATCAGA TGTTTCTGAT CAAGAGGAAG ATGAAGAAAG TGAAGAATGT 
CCGGTGTCTA TTAACTTGTC CAAAGCTGAA ACTCAGGCTT TGACTAATTA TGGAAGTGGA 
GAAGATGAGA ATGAGGATGA AGAAATAGAA GAATTTGAAG AGGGACCTGT GGATGTCCAG 
ACGTCACTTC AGGCTAACAC TGAAGCTACA GAAGAAACTG AACACGATGA TCAGGTACTA 
CAACATGACT TTGAAAAATC AGGAGAAAGC AAAAATGTCC CATCAGAACA AGATCCCACT 
ACTAGTAAAG GCAAGAAATA TGACCAAGAT AGTACTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAA ATGAGCAGCC TTTGAATAGC GCTGTCCAAA AAGACTCTCT GACTACCATC 
GATTCATCTA AACAGCCTAA CCCTTTGCCT TTACCTTTAC CTGAAATCGA AACCTTGGTG 
CCTACAGTCA AAGAAGTTAA ATCTGCTCAA GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAACATAAGT 
CAAAAATCTG ATGAAGAAGA CTTTGTGAAA GTTGAAGATT TACCACTTAA ACTGACAATA 
TACTCAGAGG CAGATCTAAG GAAGAAAATG GTAGAAGAGC AAGAGAAAAA CCATTTATCT 
GGTGAAATAC TATGTGAAAT GCAGACCGAA GAATTAGCTG GGAATTCTCA GACACTAAAA 
GAACCTGAAA CAGTGGGAGC CCAAAGCGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975616.1
CDS	616..7188
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLDCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_014120141.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120141.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGAT 
TGTCACTATA ATAGAGAAGG AGAACAGGGG ATTCATGGTG CACAAGGTGA AGACGATGAG 
GAAGAAGAAG AAGCAGAAGA TGAAGGAGTC AGTGGGGCTT CATTAACTAG TCATAGGAGC 
AGTCTGGTTG ATGAGGCTGC AGAAGACGCT GAATTTGAAC AGAAGATCAA TAGACTTATG 
GCTGCCAAAC AGAAATTAAG ACAGTTACAA GATCTTGTTG CTATGGTACA GGATGATGAT 
GCAGCAGATC ATGGAGTTAT CTCTGCCAAT ACTTCAAATT TGGATGATTT TTACCCAGCA 
GAAGAAGACA ACAAACAAAG TGCAAATAAT ACTAGAGGAA ATGCCAATAA AACACAGAAA 
GATGCTGGAA TAAATGAGAA GGCAAGAGAG AAGTTTTATG AGGCTAAACT ACAGCAGCAA 
CAGAGAGAGC TCAGACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA 
ATTCAAGCAT TACAGAAGGC ATGCCCTGAC TTACAGCTGT CAGCCACTAG TGCGGGTAAT 
TGTCCCACAA AAAAATATAT CCCAGCTGTT ACTTCAACCC CAGTTGTTAA TGGGAATGAA 
ACCAGTACAA GCAAATCTGC TTTTGAGCCT GCAGATCCTT CAGGAGTGGA TAATGAGCTG 
TGGTCAGAAA TGCGAAGACA TGAGATGTTA AGGGAAGAGC TACGGCAGAG AAGAAAGCAG 
CTTGAAGCAC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TAGCTGAAAC TACATCCCCT 
CTGGCTGTGT CATTGAGAAG TGATGGATCT GAGAACCTGT GTACGCCTCA ACAGAGTAGA 
ACAGAAAAAA CCATGGCAAC ATGGGGAGGT TCTACCCAGT GTGCACTAGA TGAAGAAGGA 
GATGAAGATG GTTACCTTTC TGAAGGAGTT GTTCGGACAG ATGAAGAGGA GGAAGAAGAA 
GAGCAAGATG CAAGTTCGAA TGATAACTTT TCTATGTATC CTCCTAACAG TGCGAATCAT 
AACTCTTACA ACATAAAAGA AACTAAGAAT AGGTGGAAGA ATAGTCGCCC TTTTACAGCA 
GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAGCAGA ATATCAGCAT GCAACGTCAG 
GAAAACCTTC GTTGGATGTC CGAACTCTCT TACGTAGAGG AGAAAGAACA ATGGCAAGAA 
CAAATTAATC AGCTGAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTGATG 
CAAGACCAGC AGACGCTGTC TTGTCTGCTG CAAACTCTTC TCACAGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTAGCTTC TCCTCAAGTA CACCTTATAA TGCACCAGTT GAATCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTGCAAAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AACAGAATCA GCACCCAGAA AAACCTGGAA GCCAGGAAAG AGGCAGTAGT 
GCACCACAGC CTTCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCATC ACAGCCTGTA 
AATCTATTTA ACCTACCCGG GTTTACTAAT TTTTCATCAT TTGCACCAGG TATGAATTTT 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAA TTTTCTCAGA ATATTTCTAC ACCTACTGAA 
CAGCAGCAAC CATTAGCCCA GAATTCTTCA GGGAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGGA 
GAGGAGGTGG AAAACAGTAG GACAGCGTGG TTATATGACC AAGAAGGTGA AGTAGAAAAA 
CCATTTATCA AGACTGGATT TCCAGTGTCT GTAGAGAAAA CTACAAATAG TAATCGCAAA 
AATCAATTAG ATACCGGCCG AAGAAGACGC CAGTTTGATG AAGAGTCATT AGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCGACA ACAGTAACCA AAACATTCAA GACGAGAAAA 
GCATCTGCAC AGGCCAGCCT AGCATCTAAA GATAAAACTC CTAAATCAAA GAGTAAGAAA 
AGGCATTCTG CTCAACTAAA AAGCAGAGTT AAAAATACTG GGTATGAAAG TGCCAGTGTG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CTTGAGCAGC TGGAAAAAAT AATAAAATAT 
AGTAGGTCTA CAGAGATATC TTCAGCACAT GCTAGGAGAA TACTACAGCA GTCTAACAGA 
AATGCATGCA ATGAAGCACC AGAAACTGGG AGTGATTTTT CCATGTTTGA AGCTTTGCGG 
GATACTATTT ATTCTGAAGT AGCTACATTA ATTTCTCAAA ATGAATCTCA TCCACATTTT 
CTTATTGAAC TCTTCCATGA ACTTCAGCTA CTTAATACAG ACTACTTGAG ACAAAGGGCT 
TTATATGCAT TGCAGGACAT AGTAACCAGA CATATTTCTG AGAACCATGA AAAAGAAGGA 
GAAAATGTAA AGTCAGTGAA TTCTGGTACT TGGATAGCAT CAAACTCAGA GCTTACCCCC 
AGTGAAAGCC TTGCTACCAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGCG TCATGTCTGT ATCTTCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACTGTGA TTCACTTAGA TCAGGCATTA 
GCCAGAATGA GGGAATACGA GCGTATGAAG ACTGAGGCTG AAAGTAGCAC AAATATAAGA 
TGCACCTGCA GAATTCTTGA GGATGAGGAT GGTGCTGCCG CCACAAGTAT GGTTACTAAT 
TTAGAAGAAA CTCCTATTGA AAATCATGGT TCACAACAAC CTGTAAGTGA AGTTTCTACT 
GTTCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC CATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCTTCTCA ACTTCTAACT 
TCAGTAAGGC GTATGGTTTT GACCCTCACC CAACAAAATG ATGAGAGCAA AGAATTTGTG 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AAGTGCAAAA GGAAAATAGA AGCAGCTGGA GTGATACAGT CTTATGCCAA AGAGGCCAAA 
AGGATTCTTG AAGGAGATCA TGGCTCACCT GCTGGAGAAA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCCGACT CAGACATCTG AGATATATGA TGGTGATGGT 
CCCAAAAATG TGAGATCAGA TGTTTCTGAT CAAGAGGAAG ATGAAGAAAG TGAAGAATGT 
CCGGTGTCTA TTAACTTGTC CAAAGCTGAA ACTCAGGCTT TGACTAATTA TGGAAGTGGA 
GAAGATGAGA ATGAGGATGA AGAAATAGAA GAATTTGAAG AGGGACCTGT GGATGTCCAG 
ACGTCACTTC AGGCTAACAC TGAAGCTACA GAAGAAACTG AACACGATGA TCAGGTACTA 
CAACATGACT TTGAAAAATC AGGAGAAAGC AAAAATGTCC CATCAGAACA AGATCCCACT 
ACTAGTAAAG GCAAGAAATA TGACCAAGAT AGTACTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAA ATGAGCAGCC TTTGAATAGC GCTGTCCAAA AAGACTCTCT GACTACCATC 
GATTCATCTA AACAGCCTAA CCCTTTGCCT TTACCTTTAC CTGAAATCGA AACCTTGGTG 
CCTACAGTCA AAGAAGTTAA ATCTGCTCAA GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAACATAAGT 
CAAAAATCTG ATGAAGAAGA CTTTGTGAAA GTTGAAGATT TACCACTTAA ACTGACAATA 
TACTCAGAGG CAGATCTAAG GAAGAAAATG GTAGAAGAGC AAGAGAAAAA CCATTTATCT 
GGTGAAATAC TATGTGAAAT GCAGACCGAA GAATTAGCTG GGAATTCTCA GACACTAAAA 
GAACCTGAAA CAGTGGGAGC CCAAAGCGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404146.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259854.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X7, mRNA.

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CloneID	OCe40623
Clone ID Related Accession (Same CDS sequence)	XM_014120141.1 , XM_022404146.1
Accession Version	XM_022404146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGAT 
TGTCACTATA ATAGAGAAGG AGAACAGGGG ATTCATGGTG CACAAGGTGA AGACGATGAG 
GAAGAAGAAG AAGCAGAAGA TGAAGGAGTC AGTGGGGCTT CATTAACTAG TCATAGGAGC 
AGTCTGGTTG ATGAGGCTGC AGAAGACGCT GAATTTGAAC AGAAGATCAA TAGACTTATG 
GCTGCCAAAC AGAAATTAAG ACAGTTACAA GATCTTGTTG CTATGGTACA GGATGATGAT 
GCAGCAGATC ATGGAGTTAT CTCTGCCAAT ACTTCAAATT TGGATGATTT TTACCCAGCA 
GAAGAAGACA ACAAACAAAG TGCAAATAAT ACTAGAGGAA ATGCCAATAA AACACAGAAA 
GATGCTGGAA TAAATGAGAA GGCAAGAGAG AAGTTTTATG AGGCTAAACT ACAGCAGCAA 
CAGAGAGAGC TCAGACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA 
ATTCAAGCAT TACAGAAGGC ATGCCCTGAC TTACAGCTGT CAGCCACTAG TGCGGGTAAT 
TGTCCCACAA AAAAATATAT CCCAGCTGTT ACTTCAACCC CAGTTGTTAA TGGGAATGAA 
ACCAGTACAA GCAAATCTGC TTTTGAGCCT GCAGATCCTT CAGGAGTGGA TAATGAGCTG 
TGGTCAGAAA TGCGAAGACA TGAGATGTTA AGGGAAGAGC TACGGCAGAG AAGAAAGCAG 
CTTGAAGCAC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TAGCTGAAAC TACATCCCCT 
CTGGCTGTGT CATTGAGAAG TGATGGATCT GAGAACCTGT GTACGCCTCA ACAGAGTAGA 
ACAGAAAAAA CCATGGCAAC ATGGGGAGGT TCTACCCAGT GTGCACTAGA TGAAGAAGGA 
GATGAAGATG GTTACCTTTC TGAAGGAGTT GTTCGGACAG ATGAAGAGGA GGAAGAAGAA 
GAGCAAGATG CAAGTTCGAA TGATAACTTT TCTATGTATC CTCCTAACAG TGCGAATCAT 
AACTCTTACA ACATAAAAGA AACTAAGAAT AGGTGGAAGA ATAGTCGCCC TTTTACAGCA 
GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAGCAGA ATATCAGCAT GCAACGTCAG 
GAAAACCTTC GTTGGATGTC CGAACTCTCT TACGTAGAGG AGAAAGAACA ATGGCAAGAA 
CAAATTAATC AGCTGAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTGATG 
CAAGACCAGC AGACGCTGTC TTGTCTGCTG CAAACTCTTC TCACAGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTAGCTTC TCCTCAAGTA CACCTTATAA TGCACCAGTT GAATCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTGCAAAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AACAGAATCA GCACCCAGAA AAACCTGGAA GCCAGGAAAG AGGCAGTAGT 
GCACCACAGC CTTCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCATC ACAGCCTGTA 
AATCTATTTA ACCTACCCGG GTTTACTAAT TTTTCATCAT TTGCACCAGG TATGAATTTT 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAA TTTTCTCAGA ATATTTCTAC ACCTACTGAA 
CAGCAGCAAC CATTAGCCCA GAATTCTTCA GGGAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGGA 
GAGGAGGTGG AAAACAGTAG GACAGCGTGG TTATATGACC AAGAAGGTGA AGTAGAAAAA 
CCATTTATCA AGACTGGATT TCCAGTGTCT GTAGAGAAAA CTACAAATAG TAATCGCAAA 
AATCAATTAG ATACCGGCCG AAGAAGACGC CAGTTTGATG AAGAGTCATT AGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCGACA ACAGTAACCA AAACATTCAA GACGAGAAAA 
GCATCTGCAC AGGCCAGCCT AGCATCTAAA GATAAAACTC CTAAATCAAA GAGTAAGAAA 
AGGCATTCTG CTCAACTAAA AAGCAGAGTT AAAAATACTG GGTATGAAAG TGCCAGTGTG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CTTGAGCAGC TGGAAAAAAT AATAAAATAT 
AGTAGGTCTA CAGAGATATC TTCAGCACAT GCTAGGAGAA TACTACAGCA GTCTAACAGA 
AATGCATGCA ATGAAGCACC AGAAACTGGG AGTGATTTTT CCATGTTTGA AGCTTTGCGG 
GATACTATTT ATTCTGAAGT AGCTACATTA ATTTCTCAAA ATGAATCTCA TCCACATTTT 
CTTATTGAAC TCTTCCATGA ACTTCAGCTA CTTAATACAG ACTACTTGAG ACAAAGGGCT 
TTATATGCAT TGCAGGACAT AGTAACCAGA CATATTTCTG AGAACCATGA AAAAGAAGGA 
GAAAATGTAA AGTCAGTGAA TTCTGGTACT TGGATAGCAT CAAACTCAGA GCTTACCCCC 
AGTGAAAGCC TTGCTACCAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGCG TCATGTCTGT ATCTTCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACTGTGA TTCACTTAGA TCAGGCATTA 
GCCAGAATGA GGGAATACGA GCGTATGAAG ACTGAGGCTG AAAGTAGCAC AAATATAAGA 
TGCACCTGCA GAATTCTTGA GGATGAGGAT GGTGCTGCCG CCACAAGTAT GGTTACTAAT 
TTAGAAGAAA CTCCTATTGA AAATCATGGT TCACAACAAC CTGTAAGTGA AGTTTCTACT 
GTTCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC CATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCTTCTCA ACTTCTAACT 
TCAGTAAGGC GTATGGTTTT GACCCTCACC CAACAAAATG ATGAGAGCAA AGAATTTGTG 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AAGTGCAAAA GGAAAATAGA AGCAGCTGGA GTGATACAGT CTTATGCCAA AGAGGCCAAA 
AGGATTCTTG AAGGAGATCA TGGCTCACCT GCTGGAGAAA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCCGACT CAGACATCTG AGATATATGA TGGTGATGGT 
CCCAAAAATG TGAGATCAGA TGTTTCTGAT CAAGAGGAAG ATGAAGAAAG TGAAGAATGT 
CCGGTGTCTA TTAACTTGTC CAAAGCTGAA ACTCAGGCTT TGACTAATTA TGGAAGTGGA 
GAAGATGAGA ATGAGGATGA AGAAATAGAA GAATTTGAAG AGGGACCTGT GGATGTCCAG 
ACGTCACTTC AGGCTAACAC TGAAGCTACA GAAGAAACTG AACACGATGA TCAGGTACTA 
CAACATGACT TTGAAAAATC AGGAGAAAGC AAAAATGTCC CATCAGAACA AGATCCCACT 
ACTAGTAAAG GCAAGAAATA TGACCAAGAT AGTACTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAA ATGAGCAGCC TTTGAATAGC GCTGTCCAAA AAGACTCTCT GACTACCATC 
GATTCATCTA AACAGCCTAA CCCTTTGCCT TTACCTTTAC CTGAAATCGA AACCTTGGTG 
CCTACAGTCA AAGAAGTTAA ATCTGCTCAA GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAACATAAGT 
CAAAAATCTG ATGAAGAAGA CTTTGTGAAA GTTGAAGATT TACCACTTAA ACTGACAATA 
TACTCAGAGG CAGATCTAAG GAAGAAAATG GTAGAAGAGC AAGAGAAAAA CCATTTATCT 
GGTGAAATAC TATGTGAAAT GCAGACCGAA GAATTAGCTG GGAATTCTCA GACACTAAAA 
GAACCTGAAA CAGTGGGAGC CCAAAGCGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259854.1
CDS	426..6998
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLDCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404146.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404146.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGAT 
TGTCACTATA ATAGAGAAGG AGAACAGGGG ATTCATGGTG CACAAGGTGA AGACGATGAG 
GAAGAAGAAG AAGCAGAAGA TGAAGGAGTC AGTGGGGCTT CATTAACTAG TCATAGGAGC 
AGTCTGGTTG ATGAGGCTGC AGAAGACGCT GAATTTGAAC AGAAGATCAA TAGACTTATG 
GCTGCCAAAC AGAAATTAAG ACAGTTACAA GATCTTGTTG CTATGGTACA GGATGATGAT 
GCAGCAGATC ATGGAGTTAT CTCTGCCAAT ACTTCAAATT TGGATGATTT TTACCCAGCA 
GAAGAAGACA ACAAACAAAG TGCAAATAAT ACTAGAGGAA ATGCCAATAA AACACAGAAA 
GATGCTGGAA TAAATGAGAA GGCAAGAGAG AAGTTTTATG AGGCTAAACT ACAGCAGCAA 
CAGAGAGAGC TCAGACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA 
ATTCAAGCAT TACAGAAGGC ATGCCCTGAC TTACAGCTGT CAGCCACTAG TGCGGGTAAT 
TGTCCCACAA AAAAATATAT CCCAGCTGTT ACTTCAACCC CAGTTGTTAA TGGGAATGAA 
ACCAGTACAA GCAAATCTGC TTTTGAGCCT GCAGATCCTT CAGGAGTGGA TAATGAGCTG 
TGGTCAGAAA TGCGAAGACA TGAGATGTTA AGGGAAGAGC TACGGCAGAG AAGAAAGCAG 
CTTGAAGCAC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TAGCTGAAAC TACATCCCCT 
CTGGCTGTGT CATTGAGAAG TGATGGATCT GAGAACCTGT GTACGCCTCA ACAGAGTAGA 
ACAGAAAAAA CCATGGCAAC ATGGGGAGGT TCTACCCAGT GTGCACTAGA TGAAGAAGGA 
GATGAAGATG GTTACCTTTC TGAAGGAGTT GTTCGGACAG ATGAAGAGGA GGAAGAAGAA 
GAGCAAGATG CAAGTTCGAA TGATAACTTT TCTATGTATC CTCCTAACAG TGCGAATCAT 
AACTCTTACA ACATAAAAGA AACTAAGAAT AGGTGGAAGA ATAGTCGCCC TTTTACAGCA 
GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAGCAGA ATATCAGCAT GCAACGTCAG 
GAAAACCTTC GTTGGATGTC CGAACTCTCT TACGTAGAGG AGAAAGAACA ATGGCAAGAA 
CAAATTAATC AGCTGAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTGATG 
CAAGACCAGC AGACGCTGTC TTGTCTGCTG CAAACTCTTC TCACAGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTAGCTTC TCCTCAAGTA CACCTTATAA TGCACCAGTT GAATCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTGCAAAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AACAGAATCA GCACCCAGAA AAACCTGGAA GCCAGGAAAG AGGCAGTAGT 
GCACCACAGC CTTCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCATC ACAGCCTGTA 
AATCTATTTA ACCTACCCGG GTTTACTAAT TTTTCATCAT TTGCACCAGG TATGAATTTT 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAA TTTTCTCAGA ATATTTCTAC ACCTACTGAA 
CAGCAGCAAC CATTAGCCCA GAATTCTTCA GGGAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGGA 
GAGGAGGTGG AAAACAGTAG GACAGCGTGG TTATATGACC AAGAAGGTGA AGTAGAAAAA 
CCATTTATCA AGACTGGATT TCCAGTGTCT GTAGAGAAAA CTACAAATAG TAATCGCAAA 
AATCAATTAG ATACCGGCCG AAGAAGACGC CAGTTTGATG AAGAGTCATT AGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCGACA ACAGTAACCA AAACATTCAA GACGAGAAAA 
GCATCTGCAC AGGCCAGCCT AGCATCTAAA GATAAAACTC CTAAATCAAA GAGTAAGAAA 
AGGCATTCTG CTCAACTAAA AAGCAGAGTT AAAAATACTG GGTATGAAAG TGCCAGTGTG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CTTGAGCAGC TGGAAAAAAT AATAAAATAT 
AGTAGGTCTA CAGAGATATC TTCAGCACAT GCTAGGAGAA TACTACAGCA GTCTAACAGA 
AATGCATGCA ATGAAGCACC AGAAACTGGG AGTGATTTTT CCATGTTTGA AGCTTTGCGG 
GATACTATTT ATTCTGAAGT AGCTACATTA ATTTCTCAAA ATGAATCTCA TCCACATTTT 
CTTATTGAAC TCTTCCATGA ACTTCAGCTA CTTAATACAG ACTACTTGAG ACAAAGGGCT 
TTATATGCAT TGCAGGACAT AGTAACCAGA CATATTTCTG AGAACCATGA AAAAGAAGGA 
GAAAATGTAA AGTCAGTGAA TTCTGGTACT TGGATAGCAT CAAACTCAGA GCTTACCCCC 
AGTGAAAGCC TTGCTACCAC TGATGATGAA ACTTTTGAGA AGAACTTTGA GAGAGAAACA 
CATAAAATAA GTGAGCAAAA TGATGCTGAT AATGCTAGCG TCATGTCTGT ATCTTCAAAT 
TTTGAGCCTT TTGCAACAGA TGATCTAGGT AACACTGTGA TTCACTTAGA TCAGGCATTA 
GCCAGAATGA GGGAATACGA GCGTATGAAG ACTGAGGCTG AAAGTAGCAC AAATATAAGA 
TGCACCTGCA GAATTCTTGA GGATGAGGAT GGTGCTGCCG CCACAAGTAT GGTTACTAAT 
TTAGAAGAAA CTCCTATTGA AAATCATGGT TCACAACAAC CTGTAAGTGA AGTTTCTACT 
GTTCCATGTC CTAGAATTGA TACTCAGCAG CTGGACCGGC AAATTAAAGC CATTATGAAA 
GAAGTCATTC CTTTTTTGAA GGAGCACATG GATGAAGTAT GCTCTTCTCA ACTTCTAACT 
TCAGTAAGGC GTATGGTTTT GACCCTCACC CAACAAAATG ATGAGAGCAA AGAATTTGTG 
AAGTTCTTTC ATAAACAACT TGGAAGTATA TTACAGGATT CACTGGCAAA ATTTGCTGGC 
AGAAAACTGA AAGACTGTGG AGAAGATCTT CTTGTAGAGA TATCTGAAGT GTTGTTTAAT 
GAATTGGCTT TCTTTAAGCT CATGCAAGAT TTGGATAATA ATAGTATAAC TGTTAAACAG 
AAGTGCAAAA GGAAAATAGA AGCAGCTGGA GTGATACAGT CTTATGCCAA AGAGGCCAAA 
AGGATTCTTG AAGGAGATCA TGGCTCACCT GCTGGAGAAA TTGATGATGA AGACAAAGAC 
AAGGATGAAA CTGAAACAGT TAAGCCGACT CAGACATCTG AGATATATGA TGGTGATGGT 
CCCAAAAATG TGAGATCAGA TGTTTCTGAT CAAGAGGAAG ATGAAGAAAG TGAAGAATGT 
CCGGTGTCTA TTAACTTGTC CAAAGCTGAA ACTCAGGCTT TGACTAATTA TGGAAGTGGA 
GAAGATGAGA ATGAGGATGA AGAAATAGAA GAATTTGAAG AGGGACCTGT GGATGTCCAG 
ACGTCACTTC AGGCTAACAC TGAAGCTACA GAAGAAACTG AACACGATGA TCAGGTACTA 
CAACATGACT TTGAAAAATC AGGAGAAAGC AAAAATGTCC CATCAGAACA AGATCCCACT 
ACTAGTAAAG GCAAGAAATA TGACCAAGAT AGTACTCCTG TGAAACCCTG TTACCTCAAT 
ATCTTGGAAA ATGAGCAGCC TTTGAATAGC GCTGTCCAAA AAGACTCTCT GACTACCATC 
GATTCATCTA AACAGCCTAA CCCTTTGCCT TTACCTTTAC CTGAAATCGA AACCTTGGTG 
CCTACAGTCA AAGAAGTTAA ATCTGCTCAA GAAACTCCTG AAAGCTCTCT GGCTGGAAGT 
CCTGATACTG AATCTCCAGT GTTAGTGAAT GACTATGAAG CAGAATCTGG TAACATAAGT 
CAAAAATCTG ATGAAGAAGA CTTTGTGAAA GTTGAAGATT TACCACTTAA ACTGACAATA 
TACTCAGAGG CAGATCTAAG GAAGAAAATG GTAGAAGAGC AAGAGAAAAA CCATTTATCT 
GGTGAAATAC TATGTGAAAT GCAGACCGAA GAATTAGCTG GGAATTCTCA GACACTAAAA 
GAACCTGAAA CAGTGGGAGC CCAAAGCGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_014120142.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975617.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X11, mRNA.

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CloneID	OCe40624
Clone ID Related Accession (Same CDS sequence)	XM_014120142.1 , XM_022404149.1
Accession Version	XM_014120142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGAT 
TGTCACTATA ATAGAGAAGG AGAACAGGGG ATTCATGGTG CACAAGGTGA AGACGATGAG 
GAAGAAGAAG AAGCAGAAGA TGAAGGAGTC AGTGGGGCTT CATTAACTAG TCATAGGAGC 
AGTCTGGTTG ATGAGGCTGC AGAAGACGCT GAATTTGAAC AGAAGATCAA TAGACTTATG 
GCTGCCAAAC AGAAATTAAG ACAGTTACAA GATCTTGTTG CTATGGTACA GGATGATGAT 
GCAGCAGATC ATGGAGTTAT CTCTGCCAAT ACTTCAAATT TGGATGATTT TTACCCAGCA 
GAAGAAGACA ACAAACAAAG TGCAAATAAT ACTAGAGGAA ATGCCAATAA AACACAGAAA 
GATGCTGGAA TAAATGAGAA GGCAAGAGAG AAGTTTTATG AGGCTAAACT ACAGCAGCAA 
CAGAGAGAGC TCAGACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA 
ATTCAAGCAT TACAGAAGGC ATGCCCTGAC TTACAGCTGT CAGCCACTAG TGCGGGTAAT 
TGTCCCACAA AAAAATATAT CCCAGCTGTT ACTTCAACCC CAGTTGTTAA TGGGAATGAA 
ACCAGTACAA GCAAATCTGC TTTTGAGCCT GCAGATCCTT CAGGAGTGGA TAATGAGCTG 
TGGTCAGAAA TGCGAAGACA TGAGATGTTA AGGGAAGAGC TACGGCAGAG AAGAAAGCAG 
CTTGAAGCAC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TAGCTGAAAC TACATCCCCT 
CTGGCTGTGT CATTGAGAAG TGATGGATCT GAGAACCTGT GTACGCCTCA ACAGAGTAGA 
ACAGAAAAAA CCATGGCAAC ATGGGGAGGT TCTACCCAGT GTGCACTAGA TGAAGAAGGA 
GATGAAGATG GTTACCTTTC TGAAGGAGTT GTTCGGACAG ATGAAGAGGA GGAAGAAGAA 
GAGCAAGATG CAAGTTCGAA TGATAACTTT TCTATGTATC CTCCTAACAG TGCGAATCAT 
AACTCTTACA ACATAAAAGA AACTAAGAAT AGGTGGAAGA ATAGTCGCCC TTTTACAGCA 
GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAGCAGA ATATCAGCAT GCAACGTCAG 
GAAAACCTTC GTTGGATGTC CGAACTCTCT TACGTAGAGG AGAAAGAACA ATGGCAAGAA 
CAAATTAATC AGCTGAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTGATG 
CAAGACCAGC AGACGCTGTC TTGTCTGCTG CAAACTCTTC TCACAGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTAGCTTC TCCTCAAGTA CACCTTATAA TGCACCAGTT GAATCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTGCAAAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AACAGAATCA GCACCCAGAA AAACCTGGAA GCCAGGAAAG AGGCAGTAGT 
GCACCACAGC CTTCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCATC ACAGCCTGTA 
AATCTATTTA ACCTACCCGG GTTTACTAAT TTTTCATCAT TTGCACCAGG TATGAATTTT 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAA TTTTCTCAGA ATATTTCTAC ACCTACTGAA 
CAGCAGCAAC CATTAGCCCA GAATTCTTCA GGGAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGGA 
GAGGAGGTGG AAAACAGTAG GACAGCGTGG TTATATGACC AAGAAGGTGA AGTAGAAAAA 
CCATTTATCA AGACTGGATT TCCAGTGTCT GTAGAGAAAA CTACAAATAG TAATCGCAAA 
AATCAATTAG ATACCGGCCG AAGAAGACGC CAGTTTGATG AAGAGTCATT AGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCGACA ACAGTAACCA AAACATTCAA GACGAGAAAA 
GCATCTGCAC AGGCCAGCCT AGCATCTAAA GATAAAACTC CTAAATCAAA GAGTAAGAAA 
AGGCATTCTG CTCAACTAAA AAGCAGAGTT AAAAATACTG GGTATGAAAG TGCCAGTGTG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CTTGAGCAGC TGGAAAAAAT AATAAAATAT 
AGTAGGTCTA CAGAGATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975617.1
CDS	616..7131
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLDCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_014120142.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120142.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGAT 
TGTCACTATA ATAGAGAAGG AGAACAGGGG ATTCATGGTG CACAAGGTGA AGACGATGAG 
GAAGAAGAAG AAGCAGAAGA TGAAGGAGTC AGTGGGGCTT CATTAACTAG TCATAGGAGC 
AGTCTGGTTG ATGAGGCTGC AGAAGACGCT GAATTTGAAC AGAAGATCAA TAGACTTATG 
GCTGCCAAAC AGAAATTAAG ACAGTTACAA GATCTTGTTG CTATGGTACA GGATGATGAT 
GCAGCAGATC ATGGAGTTAT CTCTGCCAAT ACTTCAAATT TGGATGATTT TTACCCAGCA 
GAAGAAGACA ACAAACAAAG TGCAAATAAT ACTAGAGGAA ATGCCAATAA AACACAGAAA 
GATGCTGGAA TAAATGAGAA GGCAAGAGAG AAGTTTTATG AGGCTAAACT ACAGCAGCAA 
CAGAGAGAGC TCAGACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA 
ATTCAAGCAT TACAGAAGGC ATGCCCTGAC TTACAGCTGT CAGCCACTAG TGCGGGTAAT 
TGTCCCACAA AAAAATATAT CCCAGCTGTT ACTTCAACCC CAGTTGTTAA TGGGAATGAA 
ACCAGTACAA GCAAATCTGC TTTTGAGCCT GCAGATCCTT CAGGAGTGGA TAATGAGCTG 
TGGTCAGAAA TGCGAAGACA TGAGATGTTA AGGGAAGAGC TACGGCAGAG AAGAAAGCAG 
CTTGAAGCAC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TAGCTGAAAC TACATCCCCT 
CTGGCTGTGT CATTGAGAAG TGATGGATCT GAGAACCTGT GTACGCCTCA ACAGAGTAGA 
ACAGAAAAAA CCATGGCAAC ATGGGGAGGT TCTACCCAGT GTGCACTAGA TGAAGAAGGA 
GATGAAGATG GTTACCTTTC TGAAGGAGTT GTTCGGACAG ATGAAGAGGA GGAAGAAGAA 
GAGCAAGATG CAAGTTCGAA TGATAACTTT TCTATGTATC CTCCTAACAG TGCGAATCAT 
AACTCTTACA ACATAAAAGA AACTAAGAAT AGGTGGAAGA ATAGTCGCCC TTTTACAGCA 
GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAGCAGA ATATCAGCAT GCAACGTCAG 
GAAAACCTTC GTTGGATGTC CGAACTCTCT TACGTAGAGG AGAAAGAACA ATGGCAAGAA 
CAAATTAATC AGCTGAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTGATG 
CAAGACCAGC AGACGCTGTC TTGTCTGCTG CAAACTCTTC TCACAGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTAGCTTC TCCTCAAGTA CACCTTATAA TGCACCAGTT GAATCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTGCAAAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AACAGAATCA GCACCCAGAA AAACCTGGAA GCCAGGAAAG AGGCAGTAGT 
GCACCACAGC CTTCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCATC ACAGCCTGTA 
AATCTATTTA ACCTACCCGG GTTTACTAAT TTTTCATCAT TTGCACCAGG TATGAATTTT 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAA TTTTCTCAGA ATATTTCTAC ACCTACTGAA 
CAGCAGCAAC CATTAGCCCA GAATTCTTCA GGGAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGGA 
GAGGAGGTGG AAAACAGTAG GACAGCGTGG TTATATGACC AAGAAGGTGA AGTAGAAAAA 
CCATTTATCA AGACTGGATT TCCAGTGTCT GTAGAGAAAA CTACAAATAG TAATCGCAAA 
AATCAATTAG ATACCGGCCG AAGAAGACGC CAGTTTGATG AAGAGTCATT AGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCGACA ACAGTAACCA AAACATTCAA GACGAGAAAA 
GCATCTGCAC AGGCCAGCCT AGCATCTAAA GATAAAACTC CTAAATCAAA GAGTAAGAAA 
AGGCATTCTG CTCAACTAAA AAGCAGAGTT AAAAATACTG GGTATGAAAG TGCCAGTGTG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CTTGAGCAGC TGGAAAAAAT AATAAAATAT 
AGTAGGTCTA CAGAGATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404149.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259857.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X10, mRNA.

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CloneID	OCe40624
Clone ID Related Accession (Same CDS sequence)	XM_014120142.1 , XM_022404149.1
Accession Version	XM_022404149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGAT 
TGTCACTATA ATAGAGAAGG AGAACAGGGG ATTCATGGTG CACAAGGTGA AGACGATGAG 
GAAGAAGAAG AAGCAGAAGA TGAAGGAGTC AGTGGGGCTT CATTAACTAG TCATAGGAGC 
AGTCTGGTTG ATGAGGCTGC AGAAGACGCT GAATTTGAAC AGAAGATCAA TAGACTTATG 
GCTGCCAAAC AGAAATTAAG ACAGTTACAA GATCTTGTTG CTATGGTACA GGATGATGAT 
GCAGCAGATC ATGGAGTTAT CTCTGCCAAT ACTTCAAATT TGGATGATTT TTACCCAGCA 
GAAGAAGACA ACAAACAAAG TGCAAATAAT ACTAGAGGAA ATGCCAATAA AACACAGAAA 
GATGCTGGAA TAAATGAGAA GGCAAGAGAG AAGTTTTATG AGGCTAAACT ACAGCAGCAA 
CAGAGAGAGC TCAGACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA 
ATTCAAGCAT TACAGAAGGC ATGCCCTGAC TTACAGCTGT CAGCCACTAG TGCGGGTAAT 
TGTCCCACAA AAAAATATAT CCCAGCTGTT ACTTCAACCC CAGTTGTTAA TGGGAATGAA 
ACCAGTACAA GCAAATCTGC TTTTGAGCCT GCAGATCCTT CAGGAGTGGA TAATGAGCTG 
TGGTCAGAAA TGCGAAGACA TGAGATGTTA AGGGAAGAGC TACGGCAGAG AAGAAAGCAG 
CTTGAAGCAC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TAGCTGAAAC TACATCCCCT 
CTGGCTGTGT CATTGAGAAG TGATGGATCT GAGAACCTGT GTACGCCTCA ACAGAGTAGA 
ACAGAAAAAA CCATGGCAAC ATGGGGAGGT TCTACCCAGT GTGCACTAGA TGAAGAAGGA 
GATGAAGATG GTTACCTTTC TGAAGGAGTT GTTCGGACAG ATGAAGAGGA GGAAGAAGAA 
GAGCAAGATG CAAGTTCGAA TGATAACTTT TCTATGTATC CTCCTAACAG TGCGAATCAT 
AACTCTTACA ACATAAAAGA AACTAAGAAT AGGTGGAAGA ATAGTCGCCC TTTTACAGCA 
GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAGCAGA ATATCAGCAT GCAACGTCAG 
GAAAACCTTC GTTGGATGTC CGAACTCTCT TACGTAGAGG AGAAAGAACA ATGGCAAGAA 
CAAATTAATC AGCTGAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTGATG 
CAAGACCAGC AGACGCTGTC TTGTCTGCTG CAAACTCTTC TCACAGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTAGCTTC TCCTCAAGTA CACCTTATAA TGCACCAGTT GAATCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTGCAAAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AACAGAATCA GCACCCAGAA AAACCTGGAA GCCAGGAAAG AGGCAGTAGT 
GCACCACAGC CTTCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCATC ACAGCCTGTA 
AATCTATTTA ACCTACCCGG GTTTACTAAT TTTTCATCAT TTGCACCAGG TATGAATTTT 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAA TTTTCTCAGA ATATTTCTAC ACCTACTGAA 
CAGCAGCAAC CATTAGCCCA GAATTCTTCA GGGAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGGA 
GAGGAGGTGG AAAACAGTAG GACAGCGTGG TTATATGACC AAGAAGGTGA AGTAGAAAAA 
CCATTTATCA AGACTGGATT TCCAGTGTCT GTAGAGAAAA CTACAAATAG TAATCGCAAA 
AATCAATTAG ATACCGGCCG AAGAAGACGC CAGTTTGATG AAGAGTCATT AGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCGACA ACAGTAACCA AAACATTCAA GACGAGAAAA 
GCATCTGCAC AGGCCAGCCT AGCATCTAAA GATAAAACTC CTAAATCAAA GAGTAAGAAA 
AGGCATTCTG CTCAACTAAA AAGCAGAGTT AAAAATACTG GGTATGAAAG TGCCAGTGTG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CTTGAGCAGC TGGAAAAAAT AATAAAATAT 
AGTAGGTCTA CAGAGATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259857.1
CDS	426..6941
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLDCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404149.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404149.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGAT 
TGTCACTATA ATAGAGAAGG AGAACAGGGG ATTCATGGTG CACAAGGTGA AGACGATGAG 
GAAGAAGAAG AAGCAGAAGA TGAAGGAGTC AGTGGGGCTT CATTAACTAG TCATAGGAGC 
AGTCTGGTTG ATGAGGCTGC AGAAGACGCT GAATTTGAAC AGAAGATCAA TAGACTTATG 
GCTGCCAAAC AGAAATTAAG ACAGTTACAA GATCTTGTTG CTATGGTACA GGATGATGAT 
GCAGCAGATC ATGGAGTTAT CTCTGCCAAT ACTTCAAATT TGGATGATTT TTACCCAGCA 
GAAGAAGACA ACAAACAAAG TGCAAATAAT ACTAGAGGAA ATGCCAATAA AACACAGAAA 
GATGCTGGAA TAAATGAGAA GGCAAGAGAG AAGTTTTATG AGGCTAAACT ACAGCAGCAA 
CAGAGAGAGC TCAGACAATT GCAGGAAGAA AGAAAGAAAC TGATTGAGAT TCAAGAGAAA 
ATTCAAGCAT TACAGAAGGC ATGCCCTGAC TTACAGCTGT CAGCCACTAG TGCGGGTAAT 
TGTCCCACAA AAAAATATAT CCCAGCTGTT ACTTCAACCC CAGTTGTTAA TGGGAATGAA 
ACCAGTACAA GCAAATCTGC TTTTGAGCCT GCAGATCCTT CAGGAGTGGA TAATGAGCTG 
TGGTCAGAAA TGCGAAGACA TGAGATGTTA AGGGAAGAGC TACGGCAGAG AAGAAAGCAG 
CTTGAAGCAC TGATGGCTGA ACATCAGAGG AGGCAAGGTC TAGCTGAAAC TACATCCCCT 
CTGGCTGTGT CATTGAGAAG TGATGGATCT GAGAACCTGT GTACGCCTCA ACAGAGTAGA 
ACAGAAAAAA CCATGGCAAC ATGGGGAGGT TCTACCCAGT GTGCACTAGA TGAAGAAGGA 
GATGAAGATG GTTACCTTTC TGAAGGAGTT GTTCGGACAG ATGAAGAGGA GGAAGAAGAA 
GAGCAAGATG CAAGTTCGAA TGATAACTTT TCTATGTATC CTCCTAACAG TGCGAATCAT 
AACTCTTACA ACATAAAAGA AACTAAGAAT AGGTGGAAGA ATAGTCGCCC TTTTACAGCA 
GATGGAAATT ATCGTCCTTT AGCCAAGACA AGACAGCAGA ATATCAGCAT GCAACGTCAG 
GAAAACCTTC GTTGGATGTC CGAACTCTCT TACGTAGAGG AGAAAGAACA ATGGCAAGAA 
CAAATTAATC AGCTGAAGAA ACAACTTGAT TTCAGTGTCA ATATTTGTCA GACTTTGATG 
CAAGACCAGC AGACGCTGTC TTGTCTGCTG CAAACTCTTC TCACAGGTCC TTACAGTGTT 
ATGCCCAGCA ATGTAGCTTC TCCTCAAGTA CACCTTATAA TGCACCAGTT GAATCAGTGC 
TATACTCAGC TAACATGGCA ACAGAATAAT GTGCAAAGGT TGAAACAAAT GCTAAATGAA 
CTTATGCGCC AACAGAATCA GCACCCAGAA AAACCTGGAA GCCAGGAAAG AGGCAGTAGT 
GCACCACAGC CTTCTTCTCC CAGTTTATTT TGTCCTTTCA GCTTTCCATC ACAGCCTGTA 
AATCTATTTA ACCTACCCGG GTTTACTAAT TTTTCATCAT TTGCACCAGG TATGAATTTT 
AGCCCTTTAT TTCCTTCTAA TTTTGGAGAA TTTTCTCAGA ATATTTCTAC ACCTACTGAA 
CAGCAGCAAC CATTAGCCCA GAATTCTTCA GGGAAAACAG AATATATGGC TTTTCCAAAA 
CCTTTTGAAA GCAGTTCTTC TATTGGAGCA GAAAAACAAA GGAATCAAAA ACAGCCTGGA 
GAGGAGGTGG AAAACAGTAG GACAGCGTGG TTATATGACC AAGAAGGTGA AGTAGAAAAA 
CCATTTATCA AGACTGGATT TCCAGTGTCT GTAGAGAAAA CTACAAATAG TAATCGCAAA 
AATCAATTAG ATACCGGCCG AAGAAGACGC CAGTTTGATG AAGAGTCATT AGAAAGCTTT 
AGCAGTATGC CTGATCCAGT AGATCCGACA ACAGTAACCA AAACATTCAA GACGAGAAAA 
GCATCTGCAC AGGCCAGCCT AGCATCTAAA GATAAAACTC CTAAATCAAA GAGTAAGAAA 
AGGCATTCTG CTCAACTAAA AAGCAGAGTT AAAAATACTG GGTATGAAAG TGCCAGTGTG 
TCTAGCACAT GTGAACCTTG CAAAAGTAGG AACAGACATT CAGCCCAGAC TGAAGAGCCT 
GTTCAAGCAA AAGTATTCAG CAGAAAGAAT CTTGAGCAGC TGGAAAAAAT AATAAAATAT 
AGTAGGTCTA CAGAGATATC TTCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404156.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259864.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X17, mRNA.

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CloneID	OCe40625
Clone ID Related Accession (Same CDS sequence)	XM_022404156.1 , XM_014120144.1
Accession Version	XM_022404156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGCAGATTGT CACTATAATA GAGAAGGAGA ACAGGGGATT 
CATGGTGCAC AAGGTGAAGA CGATGAGGAA GAAGAAGAAG CAGAAGATGA AGGAGTCAGT 
GGGGCTTCAT TAACTAGTCA TAGGAGCAGT CTGGTTGATG AGGCTGCAGA AGACGCTGAA 
TTTGAACAGA AGATCAATAG ACTTATGGCT GCCAAACAGA AATTAAGACA GTTACAAGAT 
CTTGTTGCTA TGGTACAGGA TGATGATGCA GCAGATCATG GAGTTATCTC TGCCAATACT 
TCAAATTTGG ATGATTTTTA CCCAGCAGAA GAAGACAACA AACAAAGTGC AAATAATACT 
AGAGGAAATG CCAATAAAAC ACAGAAAGAT GCTGGAATAA ATGAGAAGGC AAGAGAGAAG 
TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAGCTCA GACAATTGCA GGAAGAAAGA 
AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGCATTAC AGAAGGCATG CCCTGACTTA 
CAGCTGTCAG CCACTAGTGC GGGTAATTGT CCCACAAAAA AATATATCCC AGCTGTTACT 
TCAACCCCAG TTGTTAATGG GAATGAAACC AGTACAAGCA AATCTGCTTT TGAGCCTGCA 
GATCCTTCAG GAGTGGATAA TGAGCTGTGG TCAGAAATGC GAAGACATGA GATGTTAAGG 
GAAGAGCTAC GGCAGAGAAG AAAGCAGCTT GAAGCACTGA TGGCTGAACA TCAGAGGAGG 
CAAGGTCTAG CTGAAACTAC ATCCCCTCTG GCTGTGTCAT TGAGAAGTGA TGGATCTGAG 
AACCTGTGTA CGCCTCAACA GAGTAGAACA GAAAAAACCA TGGCAACATG GGGAGGTTCT 
ACCCAGTGTG CACTAGATGA AGAAGGAGAT GAAGATGGTT ACCTTTCTGA AGGAGTTGTT 
CGGACAGATG AAGAGGAGGA AGAAGAAGAG CAAGATGCAA GTTCGAATGA TAACTTTTCT 
ATGTATCCTC CTAACAGTGC GAATCATAAC TCTTACAACA TAAAAGAAAC TAAGAATAGG 
TGGAAGAATA GTCGCCCTTT TACAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA 
CAGCAGAATA TCAGCATGCA ACGTCAGGAA AACCTTCGTT GGATGTCCGA ACTCTCTTAC 
GTAGAGGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TGAAGAAACA ACTTGATTTC 
AGTGTCAATA TTTGTCAGAC TTTGATGCAA GACCAGCAGA CGCTGTCTTG TCTGCTGCAA 
ACTCTTCTCA CAGGTCCTTA CAGTGTTATG CCCAGCAATG TAGCTTCTCC TCAAGTACAC 
CTTATAATGC ACCAGTTGAA TCAGTGCTAT ACTCAGCTAA CATGGCAACA GAATAATGTG 
CAAAGGTTGA AACAAATGCT AAATGAACTT ATGCGCCAAC AGAATCAGCA CCCAGAAAAA 
CCTGGAAGCC AGGAAAGAGG CAGTAGTGCA CCACAGCCTT CTTCTCCCAG TTTATTTTGT 
CCTTTCAGCT TTCCATCACA GCCTGTAAAT CTATTTAACC TACCCGGGTT TACTAATTTT 
TCATCATTTG CACCAGGTAT GAATTTTAGC CCTTTATTTC CTTCTAATTT TGGAGAATTT 
TCTCAGAATA TTTCTACACC TACTGAACAG CAGCAACCAT TAGCCCAGAA TTCTTCAGGG 
AAAACAGAAT ATATGGCTTT TCCAAAACCT TTTGAAAGCA GTTCTTCTAT TGGAGCAGAA 
AAACAAAGGA ATCAAAAACA GCCTGGAGAG GAGGTGGAAA ACAGTAGGAC AGCGTGGTTA 
TATGACCAAG AAGGTGAAGT AGAAAAACCA TTTATCAAGA CTGGATTTCC AGTGTCTGTA 
GAGAAAACTA CAAATAGTAA TCGCAAAAAT CAATTAGATA CCGGCCGAAG AAGACGCCAG 
TTTGATGAAG AGTCATTAGA AAGCTTTAGC AGTATGCCTG ATCCAGTAGA TCCGACAACA 
GTAACCAAAA CATTCAAGAC GAGAAAAGCA TCTGCACAGG CCAGCCTAGC ATCTAAAGAT 
AAAACTCCTA AATCAAAGAG TAAGAAAAGG CATTCTGCTC AACTAAAAAG CAGAGTTAAA 
AATACTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGGAAC 
AGACATTCAG CCCAGACTGA AGAGCCTGTT CAAGCAAAAG TATTCAGCAG AAAGAATCTT 
GAGCAGCTGG AAAAAATAAT AAAATATAGT AGGTCTACAG AGATATCTTC AGCACATGCT 
AGGAGAATAC TACAGCAGTC TAACAGAAAT GCATGCAATG AAGCACCAGA AACTGGGAGT 
GATTTTTCCA TGTTTGAAGC TTTGCGGGAT ACTATTTATT CTGAAGTAGC TACATTAATT 
TCTCAAAATG AATCTCATCC ACATTTTCTT ATTGAACTCT TCCATGAACT TCAGCTACTT 
AATACAGACT ACTTGAGACA AAGGGCTTTA TATGCATTGC AGGACATAGT AACCAGACAT 
ATTTCTGAGA ACCATGAAAA AGAAGGAGAA AATGTAAAGT CAGTGAATTC TGGTACTTGG 
ATAGCATCAA ACTCAGAGCT TACCCCCAGT GAAAGCCTTG CTACCACTGA TGATGAAACT 
TTTGAGAAGA ACTTTGAGAG AGAAACACAT AAAATAAGTG AGCAAAATGA TGCTGATAAT 
GCTAGCGTCA TGTCTGTATC TTCAAATTTT GAGCCTTTTG CAACAGATGA TCTAGGTAAC 
ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGGG AATACGAGCG TATGAAGACT 
GAGGCTGAAA GTAGCACAAA TATAAGATGC ACCTGCAGAA TTCTTGAGGA TGAGGATGGT 
GCTGCCGCCA CAAGTATGGT TACTAATTTA GAAGAAACTC CTATTGAAAA TCATGGTTCA 
CAACAACCTG TAAGTGAAGT TTCTACTGTT CCATGTCCTA GAATTGATAC TCAGCAGCTG 
GACCGGCAAA TTAAAGCCAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT 
GAAGTATGCT CTTCTCAACT TCTAACTTCA GTAAGGCGTA TGGTTTTGAC CCTCACCCAA 
CAAAATGATG AGAGCAAAGA ATTTGTGAAG TTCTTTCATA AACAACTTGG AAGTATATTA 
CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAAG ACTGTGGAGA AGATCTTCTT 
GTAGAGATAT CTGAAGTGTT GTTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG 
GATAATAATA GTATAACTGT TAAACAGAAG TGCAAAAGGA AAATAGAAGC AGCTGGAGTG 
ATACAGTCTT ATGCCAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCATGG CTCACCTGCT 
GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAAACTG AAACAGTTAA GCCGACTCAG 
ACATCTGAGA TATATGATGG TGATGGTCCC AAAAATGTGA GATCAGATGT TTCTGATCAA 
GAGGAAGATG AAGAAAGTGA AGAATGTCCG GTGTCTATTA ACTTGTCCAA AGCTGAAACT 
CAGGCTTTGA CTAATTATGG AAGTGGAGAA GATGAGAATG AGGATGAAGA AATAGAAGAA 
TTTGAAGAGG GACCTGTGGA TGTCCAGACG TCACTTCAGG CTAACACTGA AGCTACAGAA 
GAAACTGAAC ACGATGATCA GGTACTACAA CATGACTTTG AAAAATCAGG AGAAAGCAAA 
AATGTCCCAT CAGAACAAGA TCCCACTACT AGTAAAGGCA AGAAATATGA CCAAGATAGT 
ACTCCTGTGA AACCCTGTTA CCTCAATATC TTGGAAAATG AGCAGCCTTT GAATAGCGCT 
GTCCAAAAAG ACTCTCTGAC TACCATCGAT TCATCTAAAC AGCCTAACCC TTTGCCTTTA 
CCTTTACCTG AAATCGAAAC CTTGGTGCCT ACAGTCAAAG AAGTTAAATC TGCTCAAGAA 
ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC 
TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT 
GAAGATTTAC CACTTAAACT GACAATATAC TCAGAGGCAG ATCTAAGGAA GAAAATGGTA 
GAAGAGCAAG AGAAAAACCA TTTATCTGGT GAAATACTAT GTGAAATGCA GACCGAAGAA 
TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259864.1
CDS	426..6605
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404156.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404156.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGCAGATTGT CACTATAATA GAGAAGGAGA ACAGGGGATT 
CATGGTGCAC AAGGTGAAGA CGATGAGGAA GAAGAAGAAG CAGAAGATGA AGGAGTCAGT 
GGGGCTTCAT TAACTAGTCA TAGGAGCAGT CTGGTTGATG AGGCTGCAGA AGACGCTGAA 
TTTGAACAGA AGATCAATAG ACTTATGGCT GCCAAACAGA AATTAAGACA GTTACAAGAT 
CTTGTTGCTA TGGTACAGGA TGATGATGCA GCAGATCATG GAGTTATCTC TGCCAATACT 
TCAAATTTGG ATGATTTTTA CCCAGCAGAA GAAGACAACA AACAAAGTGC AAATAATACT 
AGAGGAAATG CCAATAAAAC ACAGAAAGAT GCTGGAATAA ATGAGAAGGC AAGAGAGAAG 
TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAGCTCA GACAATTGCA GGAAGAAAGA 
AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGCATTAC AGAAGGCATG CCCTGACTTA 
CAGCTGTCAG CCACTAGTGC GGGTAATTGT CCCACAAAAA AATATATCCC AGCTGTTACT 
TCAACCCCAG TTGTTAATGG GAATGAAACC AGTACAAGCA AATCTGCTTT TGAGCCTGCA 
GATCCTTCAG GAGTGGATAA TGAGCTGTGG TCAGAAATGC GAAGACATGA GATGTTAAGG 
GAAGAGCTAC GGCAGAGAAG AAAGCAGCTT GAAGCACTGA TGGCTGAACA TCAGAGGAGG 
CAAGGTCTAG CTGAAACTAC ATCCCCTCTG GCTGTGTCAT TGAGAAGTGA TGGATCTGAG 
AACCTGTGTA CGCCTCAACA GAGTAGAACA GAAAAAACCA TGGCAACATG GGGAGGTTCT 
ACCCAGTGTG CACTAGATGA AGAAGGAGAT GAAGATGGTT ACCTTTCTGA AGGAGTTGTT 
CGGACAGATG AAGAGGAGGA AGAAGAAGAG CAAGATGCAA GTTCGAATGA TAACTTTTCT 
ATGTATCCTC CTAACAGTGC GAATCATAAC TCTTACAACA TAAAAGAAAC TAAGAATAGG 
TGGAAGAATA GTCGCCCTTT TACAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA 
CAGCAGAATA TCAGCATGCA ACGTCAGGAA AACCTTCGTT GGATGTCCGA ACTCTCTTAC 
GTAGAGGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TGAAGAAACA ACTTGATTTC 
AGTGTCAATA TTTGTCAGAC TTTGATGCAA GACCAGCAGA CGCTGTCTTG TCTGCTGCAA 
ACTCTTCTCA CAGGTCCTTA CAGTGTTATG CCCAGCAATG TAGCTTCTCC TCAAGTACAC 
CTTATAATGC ACCAGTTGAA TCAGTGCTAT ACTCAGCTAA CATGGCAACA GAATAATGTG 
CAAAGGTTGA AACAAATGCT AAATGAACTT ATGCGCCAAC AGAATCAGCA CCCAGAAAAA 
CCTGGAAGCC AGGAAAGAGG CAGTAGTGCA CCACAGCCTT CTTCTCCCAG TTTATTTTGT 
CCTTTCAGCT TTCCATCACA GCCTGTAAAT CTATTTAACC TACCCGGGTT TACTAATTTT 
TCATCATTTG CACCAGGTAT GAATTTTAGC CCTTTATTTC CTTCTAATTT TGGAGAATTT 
TCTCAGAATA TTTCTACACC TACTGAACAG CAGCAACCAT TAGCCCAGAA TTCTTCAGGG 
AAAACAGAAT ATATGGCTTT TCCAAAACCT TTTGAAAGCA GTTCTTCTAT TGGAGCAGAA 
AAACAAAGGA ATCAAAAACA GCCTGGAGAG GAGGTGGAAA ACAGTAGGAC AGCGTGGTTA 
TATGACCAAG AAGGTGAAGT AGAAAAACCA TTTATCAAGA CTGGATTTCC AGTGTCTGTA 
GAGAAAACTA CAAATAGTAA TCGCAAAAAT CAATTAGATA CCGGCCGAAG AAGACGCCAG 
TTTGATGAAG AGTCATTAGA AAGCTTTAGC AGTATGCCTG ATCCAGTAGA TCCGACAACA 
GTAACCAAAA CATTCAAGAC GAGAAAAGCA TCTGCACAGG CCAGCCTAGC ATCTAAAGAT 
AAAACTCCTA AATCAAAGAG TAAGAAAAGG CATTCTGCTC AACTAAAAAG CAGAGTTAAA 
AATACTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGGAAC 
AGACATTCAG CCCAGACTGA AGAGCCTGTT CAAGCAAAAG TATTCAGCAG AAAGAATCTT 
GAGCAGCTGG AAAAAATAAT AAAATATAGT AGGTCTACAG AGATATCTTC AGCACATGCT 
AGGAGAATAC TACAGCAGTC TAACAGAAAT GCATGCAATG AAGCACCAGA AACTGGGAGT 
GATTTTTCCA TGTTTGAAGC TTTGCGGGAT ACTATTTATT CTGAAGTAGC TACATTAATT 
TCTCAAAATG AATCTCATCC ACATTTTCTT ATTGAACTCT TCCATGAACT TCAGCTACTT 
AATACAGACT ACTTGAGACA AAGGGCTTTA TATGCATTGC AGGACATAGT AACCAGACAT 
ATTTCTGAGA ACCATGAAAA AGAAGGAGAA AATGTAAAGT CAGTGAATTC TGGTACTTGG 
ATAGCATCAA ACTCAGAGCT TACCCCCAGT GAAAGCCTTG CTACCACTGA TGATGAAACT 
TTTGAGAAGA ACTTTGAGAG AGAAACACAT AAAATAAGTG AGCAAAATGA TGCTGATAAT 
GCTAGCGTCA TGTCTGTATC TTCAAATTTT GAGCCTTTTG CAACAGATGA TCTAGGTAAC 
ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGGG AATACGAGCG TATGAAGACT 
GAGGCTGAAA GTAGCACAAA TATAAGATGC ACCTGCAGAA TTCTTGAGGA TGAGGATGGT 
GCTGCCGCCA CAAGTATGGT TACTAATTTA GAAGAAACTC CTATTGAAAA TCATGGTTCA 
CAACAACCTG TAAGTGAAGT TTCTACTGTT CCATGTCCTA GAATTGATAC TCAGCAGCTG 
GACCGGCAAA TTAAAGCCAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT 
GAAGTATGCT CTTCTCAACT TCTAACTTCA GTAAGGCGTA TGGTTTTGAC CCTCACCCAA 
CAAAATGATG AGAGCAAAGA ATTTGTGAAG TTCTTTCATA AACAACTTGG AAGTATATTA 
CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAAG ACTGTGGAGA AGATCTTCTT 
GTAGAGATAT CTGAAGTGTT GTTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG 
GATAATAATA GTATAACTGT TAAACAGAAG TGCAAAAGGA AAATAGAAGC AGCTGGAGTG 
ATACAGTCTT ATGCCAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCATGG CTCACCTGCT 
GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAAACTG AAACAGTTAA GCCGACTCAG 
ACATCTGAGA TATATGATGG TGATGGTCCC AAAAATGTGA GATCAGATGT TTCTGATCAA 
GAGGAAGATG AAGAAAGTGA AGAATGTCCG GTGTCTATTA ACTTGTCCAA AGCTGAAACT 
CAGGCTTTGA CTAATTATGG AAGTGGAGAA GATGAGAATG AGGATGAAGA AATAGAAGAA 
TTTGAAGAGG GACCTGTGGA TGTCCAGACG TCACTTCAGG CTAACACTGA AGCTACAGAA 
GAAACTGAAC ACGATGATCA GGTACTACAA CATGACTTTG AAAAATCAGG AGAAAGCAAA 
AATGTCCCAT CAGAACAAGA TCCCACTACT AGTAAAGGCA AGAAATATGA CCAAGATAGT 
ACTCCTGTGA AACCCTGTTA CCTCAATATC TTGGAAAATG AGCAGCCTTT GAATAGCGCT 
GTCCAAAAAG ACTCTCTGAC TACCATCGAT TCATCTAAAC AGCCTAACCC TTTGCCTTTA 
CCTTTACCTG AAATCGAAAC CTTGGTGCCT ACAGTCAAAG AAGTTAAATC TGCTCAAGAA 
ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC 
TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT 
GAAGATTTAC CACTTAAACT GACAATATAC TCAGAGGCAG ATCTAAGGAA GAAAATGGTA 
GAAGAGCAAG AGAAAAACCA TTTATCTGGT GAAATACTAT GTGAAATGCA GACCGAAGAA 
TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_014120144.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975619.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X16, mRNA.

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CloneID	OCe40625
Clone ID Related Accession (Same CDS sequence)	XM_022404156.1 , XM_014120144.1
Accession Version	XM_014120144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGCAGATTGT CACTATAATA GAGAAGGAGA ACAGGGGATT 
CATGGTGCAC AAGGTGAAGA CGATGAGGAA GAAGAAGAAG CAGAAGATGA AGGAGTCAGT 
GGGGCTTCAT TAACTAGTCA TAGGAGCAGT CTGGTTGATG AGGCTGCAGA AGACGCTGAA 
TTTGAACAGA AGATCAATAG ACTTATGGCT GCCAAACAGA AATTAAGACA GTTACAAGAT 
CTTGTTGCTA TGGTACAGGA TGATGATGCA GCAGATCATG GAGTTATCTC TGCCAATACT 
TCAAATTTGG ATGATTTTTA CCCAGCAGAA GAAGACAACA AACAAAGTGC AAATAATACT 
AGAGGAAATG CCAATAAAAC ACAGAAAGAT GCTGGAATAA ATGAGAAGGC AAGAGAGAAG 
TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAGCTCA GACAATTGCA GGAAGAAAGA 
AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGCATTAC AGAAGGCATG CCCTGACTTA 
CAGCTGTCAG CCACTAGTGC GGGTAATTGT CCCACAAAAA AATATATCCC AGCTGTTACT 
TCAACCCCAG TTGTTAATGG GAATGAAACC AGTACAAGCA AATCTGCTTT TGAGCCTGCA 
GATCCTTCAG GAGTGGATAA TGAGCTGTGG TCAGAAATGC GAAGACATGA GATGTTAAGG 
GAAGAGCTAC GGCAGAGAAG AAAGCAGCTT GAAGCACTGA TGGCTGAACA TCAGAGGAGG 
CAAGGTCTAG CTGAAACTAC ATCCCCTCTG GCTGTGTCAT TGAGAAGTGA TGGATCTGAG 
AACCTGTGTA CGCCTCAACA GAGTAGAACA GAAAAAACCA TGGCAACATG GGGAGGTTCT 
ACCCAGTGTG CACTAGATGA AGAAGGAGAT GAAGATGGTT ACCTTTCTGA AGGAGTTGTT 
CGGACAGATG AAGAGGAGGA AGAAGAAGAG CAAGATGCAA GTTCGAATGA TAACTTTTCT 
ATGTATCCTC CTAACAGTGC GAATCATAAC TCTTACAACA TAAAAGAAAC TAAGAATAGG 
TGGAAGAATA GTCGCCCTTT TACAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA 
CAGCAGAATA TCAGCATGCA ACGTCAGGAA AACCTTCGTT GGATGTCCGA ACTCTCTTAC 
GTAGAGGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TGAAGAAACA ACTTGATTTC 
AGTGTCAATA TTTGTCAGAC TTTGATGCAA GACCAGCAGA CGCTGTCTTG TCTGCTGCAA 
ACTCTTCTCA CAGGTCCTTA CAGTGTTATG CCCAGCAATG TAGCTTCTCC TCAAGTACAC 
CTTATAATGC ACCAGTTGAA TCAGTGCTAT ACTCAGCTAA CATGGCAACA GAATAATGTG 
CAAAGGTTGA AACAAATGCT AAATGAACTT ATGCGCCAAC AGAATCAGCA CCCAGAAAAA 
CCTGGAAGCC AGGAAAGAGG CAGTAGTGCA CCACAGCCTT CTTCTCCCAG TTTATTTTGT 
CCTTTCAGCT TTCCATCACA GCCTGTAAAT CTATTTAACC TACCCGGGTT TACTAATTTT 
TCATCATTTG CACCAGGTAT GAATTTTAGC CCTTTATTTC CTTCTAATTT TGGAGAATTT 
TCTCAGAATA TTTCTACACC TACTGAACAG CAGCAACCAT TAGCCCAGAA TTCTTCAGGG 
AAAACAGAAT ATATGGCTTT TCCAAAACCT TTTGAAAGCA GTTCTTCTAT TGGAGCAGAA 
AAACAAAGGA ATCAAAAACA GCCTGGAGAG GAGGTGGAAA ACAGTAGGAC AGCGTGGTTA 
TATGACCAAG AAGGTGAAGT AGAAAAACCA TTTATCAAGA CTGGATTTCC AGTGTCTGTA 
GAGAAAACTA CAAATAGTAA TCGCAAAAAT CAATTAGATA CCGGCCGAAG AAGACGCCAG 
TTTGATGAAG AGTCATTAGA AAGCTTTAGC AGTATGCCTG ATCCAGTAGA TCCGACAACA 
GTAACCAAAA CATTCAAGAC GAGAAAAGCA TCTGCACAGG CCAGCCTAGC ATCTAAAGAT 
AAAACTCCTA AATCAAAGAG TAAGAAAAGG CATTCTGCTC AACTAAAAAG CAGAGTTAAA 
AATACTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGGAAC 
AGACATTCAG CCCAGACTGA AGAGCCTGTT CAAGCAAAAG TATTCAGCAG AAAGAATCTT 
GAGCAGCTGG AAAAAATAAT AAAATATAGT AGGTCTACAG AGATATCTTC AGCACATGCT 
AGGAGAATAC TACAGCAGTC TAACAGAAAT GCATGCAATG AAGCACCAGA AACTGGGAGT 
GATTTTTCCA TGTTTGAAGC TTTGCGGGAT ACTATTTATT CTGAAGTAGC TACATTAATT 
TCTCAAAATG AATCTCATCC ACATTTTCTT ATTGAACTCT TCCATGAACT TCAGCTACTT 
AATACAGACT ACTTGAGACA AAGGGCTTTA TATGCATTGC AGGACATAGT AACCAGACAT 
ATTTCTGAGA ACCATGAAAA AGAAGGAGAA AATGTAAAGT CAGTGAATTC TGGTACTTGG 
ATAGCATCAA ACTCAGAGCT TACCCCCAGT GAAAGCCTTG CTACCACTGA TGATGAAACT 
TTTGAGAAGA ACTTTGAGAG AGAAACACAT AAAATAAGTG AGCAAAATGA TGCTGATAAT 
GCTAGCGTCA TGTCTGTATC TTCAAATTTT GAGCCTTTTG CAACAGATGA TCTAGGTAAC 
ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGGG AATACGAGCG TATGAAGACT 
GAGGCTGAAA GTAGCACAAA TATAAGATGC ACCTGCAGAA TTCTTGAGGA TGAGGATGGT 
GCTGCCGCCA CAAGTATGGT TACTAATTTA GAAGAAACTC CTATTGAAAA TCATGGTTCA 
CAACAACCTG TAAGTGAAGT TTCTACTGTT CCATGTCCTA GAATTGATAC TCAGCAGCTG 
GACCGGCAAA TTAAAGCCAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT 
GAAGTATGCT CTTCTCAACT TCTAACTTCA GTAAGGCGTA TGGTTTTGAC CCTCACCCAA 
CAAAATGATG AGAGCAAAGA ATTTGTGAAG TTCTTTCATA AACAACTTGG AAGTATATTA 
CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAAG ACTGTGGAGA AGATCTTCTT 
GTAGAGATAT CTGAAGTGTT GTTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG 
GATAATAATA GTATAACTGT TAAACAGAAG TGCAAAAGGA AAATAGAAGC AGCTGGAGTG 
ATACAGTCTT ATGCCAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCATGG CTCACCTGCT 
GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAAACTG AAACAGTTAA GCCGACTCAG 
ACATCTGAGA TATATGATGG TGATGGTCCC AAAAATGTGA GATCAGATGT TTCTGATCAA 
GAGGAAGATG AAGAAAGTGA AGAATGTCCG GTGTCTATTA ACTTGTCCAA AGCTGAAACT 
CAGGCTTTGA CTAATTATGG AAGTGGAGAA GATGAGAATG AGGATGAAGA AATAGAAGAA 
TTTGAAGAGG GACCTGTGGA TGTCCAGACG TCACTTCAGG CTAACACTGA AGCTACAGAA 
GAAACTGAAC ACGATGATCA GGTACTACAA CATGACTTTG AAAAATCAGG AGAAAGCAAA 
AATGTCCCAT CAGAACAAGA TCCCACTACT AGTAAAGGCA AGAAATATGA CCAAGATAGT 
ACTCCTGTGA AACCCTGTTA CCTCAATATC TTGGAAAATG AGCAGCCTTT GAATAGCGCT 
GTCCAAAAAG ACTCTCTGAC TACCATCGAT TCATCTAAAC AGCCTAACCC TTTGCCTTTA 
CCTTTACCTG AAATCGAAAC CTTGGTGCCT ACAGTCAAAG AAGTTAAATC TGCTCAAGAA 
ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC 
TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT 
GAAGATTTAC CACTTAAACT GACAATATAC TCAGAGGCAG ATCTAAGGAA GAAAATGGTA 
GAAGAGCAAG AGAAAAACCA TTTATCTGGT GAAATACTAT GTGAAATGCA GACCGAAGAA 
TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975619.1
CDS	615..6794
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_014120144.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120144.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGCAGATTGT CACTATAATA GAGAAGGAGA ACAGGGGATT 
CATGGTGCAC AAGGTGAAGA CGATGAGGAA GAAGAAGAAG CAGAAGATGA AGGAGTCAGT 
GGGGCTTCAT TAACTAGTCA TAGGAGCAGT CTGGTTGATG AGGCTGCAGA AGACGCTGAA 
TTTGAACAGA AGATCAATAG ACTTATGGCT GCCAAACAGA AATTAAGACA GTTACAAGAT 
CTTGTTGCTA TGGTACAGGA TGATGATGCA GCAGATCATG GAGTTATCTC TGCCAATACT 
TCAAATTTGG ATGATTTTTA CCCAGCAGAA GAAGACAACA AACAAAGTGC AAATAATACT 
AGAGGAAATG CCAATAAAAC ACAGAAAGAT GCTGGAATAA ATGAGAAGGC AAGAGAGAAG 
TTTTATGAGG CTAAACTACA GCAGCAACAG AGAGAGCTCA GACAATTGCA GGAAGAAAGA 
AAGAAACTGA TTGAGATTCA AGAGAAAATT CAAGCATTAC AGAAGGCATG CCCTGACTTA 
CAGCTGTCAG CCACTAGTGC GGGTAATTGT CCCACAAAAA AATATATCCC AGCTGTTACT 
TCAACCCCAG TTGTTAATGG GAATGAAACC AGTACAAGCA AATCTGCTTT TGAGCCTGCA 
GATCCTTCAG GAGTGGATAA TGAGCTGTGG TCAGAAATGC GAAGACATGA GATGTTAAGG 
GAAGAGCTAC GGCAGAGAAG AAAGCAGCTT GAAGCACTGA TGGCTGAACA TCAGAGGAGG 
CAAGGTCTAG CTGAAACTAC ATCCCCTCTG GCTGTGTCAT TGAGAAGTGA TGGATCTGAG 
AACCTGTGTA CGCCTCAACA GAGTAGAACA GAAAAAACCA TGGCAACATG GGGAGGTTCT 
ACCCAGTGTG CACTAGATGA AGAAGGAGAT GAAGATGGTT ACCTTTCTGA AGGAGTTGTT 
CGGACAGATG AAGAGGAGGA AGAAGAAGAG CAAGATGCAA GTTCGAATGA TAACTTTTCT 
ATGTATCCTC CTAACAGTGC GAATCATAAC TCTTACAACA TAAAAGAAAC TAAGAATAGG 
TGGAAGAATA GTCGCCCTTT TACAGCAGAT GGAAATTATC GTCCTTTAGC CAAGACAAGA 
CAGCAGAATA TCAGCATGCA ACGTCAGGAA AACCTTCGTT GGATGTCCGA ACTCTCTTAC 
GTAGAGGAGA AAGAACAATG GCAAGAACAA ATTAATCAGC TGAAGAAACA ACTTGATTTC 
AGTGTCAATA TTTGTCAGAC TTTGATGCAA GACCAGCAGA CGCTGTCTTG TCTGCTGCAA 
ACTCTTCTCA CAGGTCCTTA CAGTGTTATG CCCAGCAATG TAGCTTCTCC TCAAGTACAC 
CTTATAATGC ACCAGTTGAA TCAGTGCTAT ACTCAGCTAA CATGGCAACA GAATAATGTG 
CAAAGGTTGA AACAAATGCT AAATGAACTT ATGCGCCAAC AGAATCAGCA CCCAGAAAAA 
CCTGGAAGCC AGGAAAGAGG CAGTAGTGCA CCACAGCCTT CTTCTCCCAG TTTATTTTGT 
CCTTTCAGCT TTCCATCACA GCCTGTAAAT CTATTTAACC TACCCGGGTT TACTAATTTT 
TCATCATTTG CACCAGGTAT GAATTTTAGC CCTTTATTTC CTTCTAATTT TGGAGAATTT 
TCTCAGAATA TTTCTACACC TACTGAACAG CAGCAACCAT TAGCCCAGAA TTCTTCAGGG 
AAAACAGAAT ATATGGCTTT TCCAAAACCT TTTGAAAGCA GTTCTTCTAT TGGAGCAGAA 
AAACAAAGGA ATCAAAAACA GCCTGGAGAG GAGGTGGAAA ACAGTAGGAC AGCGTGGTTA 
TATGACCAAG AAGGTGAAGT AGAAAAACCA TTTATCAAGA CTGGATTTCC AGTGTCTGTA 
GAGAAAACTA CAAATAGTAA TCGCAAAAAT CAATTAGATA CCGGCCGAAG AAGACGCCAG 
TTTGATGAAG AGTCATTAGA AAGCTTTAGC AGTATGCCTG ATCCAGTAGA TCCGACAACA 
GTAACCAAAA CATTCAAGAC GAGAAAAGCA TCTGCACAGG CCAGCCTAGC ATCTAAAGAT 
AAAACTCCTA AATCAAAGAG TAAGAAAAGG CATTCTGCTC AACTAAAAAG CAGAGTTAAA 
AATACTGGGT ATGAAAGTGC CAGTGTGTCT AGCACATGTG AACCTTGCAA AAGTAGGAAC 
AGACATTCAG CCCAGACTGA AGAGCCTGTT CAAGCAAAAG TATTCAGCAG AAAGAATCTT 
GAGCAGCTGG AAAAAATAAT AAAATATAGT AGGTCTACAG AGATATCTTC AGCACATGCT 
AGGAGAATAC TACAGCAGTC TAACAGAAAT GCATGCAATG AAGCACCAGA AACTGGGAGT 
GATTTTTCCA TGTTTGAAGC TTTGCGGGAT ACTATTTATT CTGAAGTAGC TACATTAATT 
TCTCAAAATG AATCTCATCC ACATTTTCTT ATTGAACTCT TCCATGAACT TCAGCTACTT 
AATACAGACT ACTTGAGACA AAGGGCTTTA TATGCATTGC AGGACATAGT AACCAGACAT 
ATTTCTGAGA ACCATGAAAA AGAAGGAGAA AATGTAAAGT CAGTGAATTC TGGTACTTGG 
ATAGCATCAA ACTCAGAGCT TACCCCCAGT GAAAGCCTTG CTACCACTGA TGATGAAACT 
TTTGAGAAGA ACTTTGAGAG AGAAACACAT AAAATAAGTG AGCAAAATGA TGCTGATAAT 
GCTAGCGTCA TGTCTGTATC TTCAAATTTT GAGCCTTTTG CAACAGATGA TCTAGGTAAC 
ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGGG AATACGAGCG TATGAAGACT 
GAGGCTGAAA GTAGCACAAA TATAAGATGC ACCTGCAGAA TTCTTGAGGA TGAGGATGGT 
GCTGCCGCCA CAAGTATGGT TACTAATTTA GAAGAAACTC CTATTGAAAA TCATGGTTCA 
CAACAACCTG TAAGTGAAGT TTCTACTGTT CCATGTCCTA GAATTGATAC TCAGCAGCTG 
GACCGGCAAA TTAAAGCCAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT 
GAAGTATGCT CTTCTCAACT TCTAACTTCA GTAAGGCGTA TGGTTTTGAC CCTCACCCAA 
CAAAATGATG AGAGCAAAGA ATTTGTGAAG TTCTTTCATA AACAACTTGG AAGTATATTA 
CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAAG ACTGTGGAGA AGATCTTCTT 
GTAGAGATAT CTGAAGTGTT GTTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG 
GATAATAATA GTATAACTGT TAAACAGAAG TGCAAAAGGA AAATAGAAGC AGCTGGAGTG 
ATACAGTCTT ATGCCAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCATGG CTCACCTGCT 
GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAAACTG AAACAGTTAA GCCGACTCAG 
ACATCTGAGA TATATGATGG TGATGGTCCC AAAAATGTGA GATCAGATGT TTCTGATCAA 
GAGGAAGATG AAGAAAGTGA AGAATGTCCG GTGTCTATTA ACTTGTCCAA AGCTGAAACT 
CAGGCTTTGA CTAATTATGG AAGTGGAGAA GATGAGAATG AGGATGAAGA AATAGAAGAA 
TTTGAAGAGG GACCTGTGGA TGTCCAGACG TCACTTCAGG CTAACACTGA AGCTACAGAA 
GAAACTGAAC ACGATGATCA GGTACTACAA CATGACTTTG AAAAATCAGG AGAAAGCAAA 
AATGTCCCAT CAGAACAAGA TCCCACTACT AGTAAAGGCA AGAAATATGA CCAAGATAGT 
ACTCCTGTGA AACCCTGTTA CCTCAATATC TTGGAAAATG AGCAGCCTTT GAATAGCGCT 
GTCCAAAAAG ACTCTCTGAC TACCATCGAT TCATCTAAAC AGCCTAACCC TTTGCCTTTA 
CCTTTACCTG AAATCGAAAC CTTGGTGCCT ACAGTCAAAG AAGTTAAATC TGCTCAAGAA 
ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC 
TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT 
GAAGATTTAC CACTTAAACT GACAATATAC TCAGAGGCAG ATCTAAGGAA GAAAATGGTA 
GAAGAGCAAG AGAAAAACCA TTTATCTGGT GAAATACTAT GTGAAATGCA GACCGAAGAA 
TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404157.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259865.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X18, mRNA.

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CloneID	OCe40626
Clone ID Related Accession (Same CDS sequence)	XM_022404157.1 , XM_014120145.1
Accession Version	XM_022404157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGATTGTCAC TATAATAGAG AAGGAGAACA GGGGATTCAT 
GGTGCACAAG GTGAAGACGA TGAGGAAGAA GAAGAAGCAG AAGATGAAGG AGTCAGTGGG 
GCTTCATTAA CTAGTCATAG GAGCAGTCTG GTTGATGAGG CTGCAGAAGA CGCTGAATTT 
GAACAGAAGA TCAATAGACT TATGGCTGCC AAACAGAAAT TAAGACAGTT ACAAGATCTT 
GTTGCTATGG TACAGGATGA TGATGCAGCA GATCATGGAG TTATCTCTGC CAATACTTCA 
AATTTGGATG ATTTTTACCC AGCAGAAGAA GACAACAAAC AAAGTGCAAA TAATACTAGA 
GGAAATGCCA ATAAAACACA GAAAGATGCT GGAATAAATG AGAAGGCAAG AGAGAAGTTT 
TATGAGGCTA AACTACAGCA GCAACAGAGA GAGCTCAGAC AATTGCAGGA AGAAAGAAAG 
AAACTGATTG AGATTCAAGA GAAAATTCAA GCATTACAGA AGGCATGCCC TGACTTACAG 
CTGTCAGCCA CTAGTGCGGG TAATTGTCCC ACAAAAAAAT ATATCCCAGC TGTTACTTCA 
ACCCCAGTTG TTAATGGGAA TGAAACCAGT ACAAGCAAAT CTGCTTTTGA GCCTGCAGAT 
CCTTCAGGAG TGGATAATGA GCTGTGGTCA GAAATGCGAA GACATGAGAT GTTAAGGGAA 
GAGCTACGGC AGAGAAGAAA GCAGCTTGAA GCACTGATGG CTGAACATCA GAGGAGGCAA 
GGTCTAGCTG AAACTACATC CCCTCTGGCT GTGTCATTGA GAAGTGATGG ATCTGAGAAC 
CTGTGTACGC CTCAACAGAG TAGAACAGAA AAAACCATGG CAACATGGGG AGGTTCTACC 
CAGTGTGCAC TAGATGAAGA AGGAGATGAA GATGGTTACC TTTCTGAAGG AGTTGTTCGG 
ACAGATGAAG AGGAGGAAGA AGAAGAGCAA GATGCAAGTT CGAATGATAA CTTTTCTATG 
TATCCTCCTA ACAGTGCGAA TCATAACTCT TACAACATAA AAGAAACTAA GAATAGGTGG 
AAGAATAGTC GCCCTTTTAC AGCAGATGGA AATTATCGTC CTTTAGCCAA GACAAGACAG 
CAGAATATCA GCATGCAACG TCAGGAAAAC CTTCGTTGGA TGTCCGAACT CTCTTACGTA 
GAGGAGAAAG AACAATGGCA AGAACAAATT AATCAGCTGA AGAAACAACT TGATTTCAGT 
GTCAATATTT GTCAGACTTT GATGCAAGAC CAGCAGACGC TGTCTTGTCT GCTGCAAACT 
CTTCTCACAG GTCCTTACAG TGTTATGCCC AGCAATGTAG CTTCTCCTCA AGTACACCTT 
ATAATGCACC AGTTGAATCA GTGCTATACT CAGCTAACAT GGCAACAGAA TAATGTGCAA 
AGGTTGAAAC AAATGCTAAA TGAACTTATG CGCCAACAGA ATCAGCACCC AGAAAAACCT 
GGAAGCCAGG AAAGAGGCAG TAGTGCACCA CAGCCTTCTT CTCCCAGTTT ATTTTGTCCT 
TTCAGCTTTC CATCACAGCC TGTAAATCTA TTTAACCTAC CCGGGTTTAC TAATTTTTCA 
TCATTTGCAC CAGGTATGAA TTTTAGCCCT TTATTTCCTT CTAATTTTGG AGAATTTTCT 
CAGAATATTT CTACACCTAC TGAACAGCAG CAACCATTAG CCCAGAATTC TTCAGGGAAA 
ACAGAATATA TGGCTTTTCC AAAACCTTTT GAAAGCAGTT CTTCTATTGG AGCAGAAAAA 
CAAAGGAATC AAAAACAGCC TGGAGAGGAG GTGGAAAACA GTAGGACAGC GTGGTTATAT 
GACCAAGAAG GTGAAGTAGA AAAACCATTT ATCAAGACTG GATTTCCAGT GTCTGTAGAG 
AAAACTACAA ATAGTAATCG CAAAAATCAA TTAGATACCG GCCGAAGAAG ACGCCAGTTT 
GATGAAGAGT CATTAGAAAG CTTTAGCAGT ATGCCTGATC CAGTAGATCC GACAACAGTA 
ACCAAAACAT TCAAGACGAG AAAAGCATCT GCACAGGCCA GCCTAGCATC TAAAGATAAA 
ACTCCTAAAT CAAAGAGTAA GAAAAGGCAT TCTGCTCAAC TAAAAAGCAG AGTTAAAAAT 
ACTGGGTATG AAAGTGCCAG TGTGTCTAGC ACATGTGAAC CTTGCAAAAG TAGGAACAGA 
CATTCAGCCC AGACTGAAGA GCCTGTTCAA GCAAAAGTAT TCAGCAGAAA GAATCTTGAG 
CAGCTGGAAA AAATAATAAA ATATAGTAGG TCTACAGAGA TATCTTCAGA AACTGGGAGT 
GATTTTTCCA TGTTTGAAGC TTTGCGGGAT ACTATTTATT CTGAAGTAGC TACATTAATT 
TCTCAAAATG AATCTCATCC ACATTTTCTT ATTGAACTCT TCCATGAACT TCAGCTACTT 
AATACAGACT ACTTGAGACA AAGGGCTTTA TATGCATTGC AGGACATAGT AACCAGACAT 
ATTTCTGAGA ACCATGAAAA AGAAGGAGAA AATGTAAAGT CAGTGAATTC TGGTACTTGG 
ATAGCATCAA ACTCAGAGCT TACCCCCAGT GAAAGCCTTG CTACCACTGA TGATGAAACT 
TTTGAGAAGA ACTTTGAGAG AGAAACACAT AAAATAAGTG AGCAAAATGA TGCTGATAAT 
GCTAGCGTCA TGTCTGTATC TTCAAATTTT GAGCCTTTTG CAACAGATGA TCTAGGTAAC 
ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGGG AATACGAGCG TATGAAGACT 
GAGGCTGAAA GTAGCACAAA TATAAGATGC ACCTGCAGAA TTCTTGAGGA TGAGGATGGT 
GCTGCCGCCA CAAGTATGGT TACTAATTTA GAAGAAACTC CTATTGAAAA TCATGGTTCA 
CAACAACCTG TAAGTGAAGT TTCTACTGTT CCATGTCCTA GAATTGATAC TCAGCAGCTG 
GACCGGCAAA TTAAAGCCAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT 
GAAGTATGCT CTTCTCAACT TCTAACTTCA GTAAGGCGTA TGGTTTTGAC CCTCACCCAA 
CAAAATGATG AGAGCAAAGA ATTTGTGAAG TTCTTTCATA AACAACTTGG AAGTATATTA 
CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAAG ACTGTGGAGA AGATCTTCTT 
GTAGAGATAT CTGAAGTGTT GTTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG 
GATAATAATA GTATAACTGT TAAACAGAAG TGCAAAAGGA AAATAGAAGC AGCTGGAGTG 
ATACAGTCTT ATGCCAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCATGG CTCACCTGCT 
GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAAACTG AAACAGTTAA GCCGACTCAG 
ACATCTGAGA TATATGATGG TGATGGTCCC AAAAATGTGA GATCAGATGT TTCTGATCAA 
GAGGAAGATG AAGAAAGTGA AGAATGTCCG GTGTCTATTA ACTTGTCCAA AGCTGAAACT 
CAGGCTTTGA CTAATTATGG AAGTGGAGAA GATGAGAATG AGGATGAAGA AATAGAAGAA 
TTTGAAGAGG GACCTGTGGA TGTCCAGACG TCACTTCAGG CTAACACTGA AGCTACAGAA 
GAAACTGAAC ACGATGATCA GGTACTACAA CATGACTTTG AAAAATCAGG AGAAAGCAAA 
AATGTCCCAT CAGAACAAGA TCCCACTACT AGTAAAGGCA AGAAATATGA CCAAGATAGT 
ACTCCTGTGA AACCCTGTTA CCTCAATATC TTGGAAAATG AGCAGCCTTT GAATAGCGCT 
GTCCAAAAAG ACTCTCTGAC TACCATCGAT TCATCTAAAC AGCCTAACCC TTTGCCTTTA 
CCTTTACCTG AAATCGAAAC CTTGGTGCCT ACAGTCAAAG AAGTTAAATC TGCTCAAGAA 
ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC 
TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT 
GAAGATTTAC CACTTAAACT GACAATATAC TCAGAGGCAG ATCTAAGGAA GAAAATGGTA 
GAAGAGCAAG AGAAAAACCA TTTATCTGGT GAAATACTAT GTGAAATGCA GACCGAAGAA 
TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259865.1
CDS	426..6545
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLDCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404157.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404157.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGATTGTCAC TATAATAGAG AAGGAGAACA GGGGATTCAT 
GGTGCACAAG GTGAAGACGA TGAGGAAGAA GAAGAAGCAG AAGATGAAGG AGTCAGTGGG 
GCTTCATTAA CTAGTCATAG GAGCAGTCTG GTTGATGAGG CTGCAGAAGA CGCTGAATTT 
GAACAGAAGA TCAATAGACT TATGGCTGCC AAACAGAAAT TAAGACAGTT ACAAGATCTT 
GTTGCTATGG TACAGGATGA TGATGCAGCA GATCATGGAG TTATCTCTGC CAATACTTCA 
AATTTGGATG ATTTTTACCC AGCAGAAGAA GACAACAAAC AAAGTGCAAA TAATACTAGA 
GGAAATGCCA ATAAAACACA GAAAGATGCT GGAATAAATG AGAAGGCAAG AGAGAAGTTT 
TATGAGGCTA AACTACAGCA GCAACAGAGA GAGCTCAGAC AATTGCAGGA AGAAAGAAAG 
AAACTGATTG AGATTCAAGA GAAAATTCAA GCATTACAGA AGGCATGCCC TGACTTACAG 
CTGTCAGCCA CTAGTGCGGG TAATTGTCCC ACAAAAAAAT ATATCCCAGC TGTTACTTCA 
ACCCCAGTTG TTAATGGGAA TGAAACCAGT ACAAGCAAAT CTGCTTTTGA GCCTGCAGAT 
CCTTCAGGAG TGGATAATGA GCTGTGGTCA GAAATGCGAA GACATGAGAT GTTAAGGGAA 
GAGCTACGGC AGAGAAGAAA GCAGCTTGAA GCACTGATGG CTGAACATCA GAGGAGGCAA 
GGTCTAGCTG AAACTACATC CCCTCTGGCT GTGTCATTGA GAAGTGATGG ATCTGAGAAC 
CTGTGTACGC CTCAACAGAG TAGAACAGAA AAAACCATGG CAACATGGGG AGGTTCTACC 
CAGTGTGCAC TAGATGAAGA AGGAGATGAA GATGGTTACC TTTCTGAAGG AGTTGTTCGG 
ACAGATGAAG AGGAGGAAGA AGAAGAGCAA GATGCAAGTT CGAATGATAA CTTTTCTATG 
TATCCTCCTA ACAGTGCGAA TCATAACTCT TACAACATAA AAGAAACTAA GAATAGGTGG 
AAGAATAGTC GCCCTTTTAC AGCAGATGGA AATTATCGTC CTTTAGCCAA GACAAGACAG 
CAGAATATCA GCATGCAACG TCAGGAAAAC CTTCGTTGGA TGTCCGAACT CTCTTACGTA 
GAGGAGAAAG AACAATGGCA AGAACAAATT AATCAGCTGA AGAAACAACT TGATTTCAGT 
GTCAATATTT GTCAGACTTT GATGCAAGAC CAGCAGACGC TGTCTTGTCT GCTGCAAACT 
CTTCTCACAG GTCCTTACAG TGTTATGCCC AGCAATGTAG CTTCTCCTCA AGTACACCTT 
ATAATGCACC AGTTGAATCA GTGCTATACT CAGCTAACAT GGCAACAGAA TAATGTGCAA 
AGGTTGAAAC AAATGCTAAA TGAACTTATG CGCCAACAGA ATCAGCACCC AGAAAAACCT 
GGAAGCCAGG AAAGAGGCAG TAGTGCACCA CAGCCTTCTT CTCCCAGTTT ATTTTGTCCT 
TTCAGCTTTC CATCACAGCC TGTAAATCTA TTTAACCTAC CCGGGTTTAC TAATTTTTCA 
TCATTTGCAC CAGGTATGAA TTTTAGCCCT TTATTTCCTT CTAATTTTGG AGAATTTTCT 
CAGAATATTT CTACACCTAC TGAACAGCAG CAACCATTAG CCCAGAATTC TTCAGGGAAA 
ACAGAATATA TGGCTTTTCC AAAACCTTTT GAAAGCAGTT CTTCTATTGG AGCAGAAAAA 
CAAAGGAATC AAAAACAGCC TGGAGAGGAG GTGGAAAACA GTAGGACAGC GTGGTTATAT 
GACCAAGAAG GTGAAGTAGA AAAACCATTT ATCAAGACTG GATTTCCAGT GTCTGTAGAG 
AAAACTACAA ATAGTAATCG CAAAAATCAA TTAGATACCG GCCGAAGAAG ACGCCAGTTT 
GATGAAGAGT CATTAGAAAG CTTTAGCAGT ATGCCTGATC CAGTAGATCC GACAACAGTA 
ACCAAAACAT TCAAGACGAG AAAAGCATCT GCACAGGCCA GCCTAGCATC TAAAGATAAA 
ACTCCTAAAT CAAAGAGTAA GAAAAGGCAT TCTGCTCAAC TAAAAAGCAG AGTTAAAAAT 
ACTGGGTATG AAAGTGCCAG TGTGTCTAGC ACATGTGAAC CTTGCAAAAG TAGGAACAGA 
CATTCAGCCC AGACTGAAGA GCCTGTTCAA GCAAAAGTAT TCAGCAGAAA GAATCTTGAG 
CAGCTGGAAA AAATAATAAA ATATAGTAGG TCTACAGAGA TATCTTCAGA AACTGGGAGT 
GATTTTTCCA TGTTTGAAGC TTTGCGGGAT ACTATTTATT CTGAAGTAGC TACATTAATT 
TCTCAAAATG AATCTCATCC ACATTTTCTT ATTGAACTCT TCCATGAACT TCAGCTACTT 
AATACAGACT ACTTGAGACA AAGGGCTTTA TATGCATTGC AGGACATAGT AACCAGACAT 
ATTTCTGAGA ACCATGAAAA AGAAGGAGAA AATGTAAAGT CAGTGAATTC TGGTACTTGG 
ATAGCATCAA ACTCAGAGCT TACCCCCAGT GAAAGCCTTG CTACCACTGA TGATGAAACT 
TTTGAGAAGA ACTTTGAGAG AGAAACACAT AAAATAAGTG AGCAAAATGA TGCTGATAAT 
GCTAGCGTCA TGTCTGTATC TTCAAATTTT GAGCCTTTTG CAACAGATGA TCTAGGTAAC 
ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGGG AATACGAGCG TATGAAGACT 
GAGGCTGAAA GTAGCACAAA TATAAGATGC ACCTGCAGAA TTCTTGAGGA TGAGGATGGT 
GCTGCCGCCA CAAGTATGGT TACTAATTTA GAAGAAACTC CTATTGAAAA TCATGGTTCA 
CAACAACCTG TAAGTGAAGT TTCTACTGTT CCATGTCCTA GAATTGATAC TCAGCAGCTG 
GACCGGCAAA TTAAAGCCAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT 
GAAGTATGCT CTTCTCAACT TCTAACTTCA GTAAGGCGTA TGGTTTTGAC CCTCACCCAA 
CAAAATGATG AGAGCAAAGA ATTTGTGAAG TTCTTTCATA AACAACTTGG AAGTATATTA 
CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAAG ACTGTGGAGA AGATCTTCTT 
GTAGAGATAT CTGAAGTGTT GTTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG 
GATAATAATA GTATAACTGT TAAACAGAAG TGCAAAAGGA AAATAGAAGC AGCTGGAGTG 
ATACAGTCTT ATGCCAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCATGG CTCACCTGCT 
GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAAACTG AAACAGTTAA GCCGACTCAG 
ACATCTGAGA TATATGATGG TGATGGTCCC AAAAATGTGA GATCAGATGT TTCTGATCAA 
GAGGAAGATG AAGAAAGTGA AGAATGTCCG GTGTCTATTA ACTTGTCCAA AGCTGAAACT 
CAGGCTTTGA CTAATTATGG AAGTGGAGAA GATGAGAATG AGGATGAAGA AATAGAAGAA 
TTTGAAGAGG GACCTGTGGA TGTCCAGACG TCACTTCAGG CTAACACTGA AGCTACAGAA 
GAAACTGAAC ACGATGATCA GGTACTACAA CATGACTTTG AAAAATCAGG AGAAAGCAAA 
AATGTCCCAT CAGAACAAGA TCCCACTACT AGTAAAGGCA AGAAATATGA CCAAGATAGT 
ACTCCTGTGA AACCCTGTTA CCTCAATATC TTGGAAAATG AGCAGCCTTT GAATAGCGCT 
GTCCAAAAAG ACTCTCTGAC TACCATCGAT TCATCTAAAC AGCCTAACCC TTTGCCTTTA 
CCTTTACCTG AAATCGAAAC CTTGGTGCCT ACAGTCAAAG AAGTTAAATC TGCTCAAGAA 
ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC 
TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT 
GAAGATTTAC CACTTAAACT GACAATATAC TCAGAGGCAG ATCTAAGGAA GAAAATGGTA 
GAAGAGCAAG AGAAAAACCA TTTATCTGGT GAAATACTAT GTGAAATGCA GACCGAAGAA 
TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_014120145.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975620.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X17, mRNA.

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CloneID	OCe40626
Clone ID Related Accession (Same CDS sequence)	XM_022404157.1 , XM_014120145.1
Accession Version	XM_014120145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGATTGTCAC TATAATAGAG AAGGAGAACA GGGGATTCAT 
GGTGCACAAG GTGAAGACGA TGAGGAAGAA GAAGAAGCAG AAGATGAAGG AGTCAGTGGG 
GCTTCATTAA CTAGTCATAG GAGCAGTCTG GTTGATGAGG CTGCAGAAGA CGCTGAATTT 
GAACAGAAGA TCAATAGACT TATGGCTGCC AAACAGAAAT TAAGACAGTT ACAAGATCTT 
GTTGCTATGG TACAGGATGA TGATGCAGCA GATCATGGAG TTATCTCTGC CAATACTTCA 
AATTTGGATG ATTTTTACCC AGCAGAAGAA GACAACAAAC AAAGTGCAAA TAATACTAGA 
GGAAATGCCA ATAAAACACA GAAAGATGCT GGAATAAATG AGAAGGCAAG AGAGAAGTTT 
TATGAGGCTA AACTACAGCA GCAACAGAGA GAGCTCAGAC AATTGCAGGA AGAAAGAAAG 
AAACTGATTG AGATTCAAGA GAAAATTCAA GCATTACAGA AGGCATGCCC TGACTTACAG 
CTGTCAGCCA CTAGTGCGGG TAATTGTCCC ACAAAAAAAT ATATCCCAGC TGTTACTTCA 
ACCCCAGTTG TTAATGGGAA TGAAACCAGT ACAAGCAAAT CTGCTTTTGA GCCTGCAGAT 
CCTTCAGGAG TGGATAATGA GCTGTGGTCA GAAATGCGAA GACATGAGAT GTTAAGGGAA 
GAGCTACGGC AGAGAAGAAA GCAGCTTGAA GCACTGATGG CTGAACATCA GAGGAGGCAA 
GGTCTAGCTG AAACTACATC CCCTCTGGCT GTGTCATTGA GAAGTGATGG ATCTGAGAAC 
CTGTGTACGC CTCAACAGAG TAGAACAGAA AAAACCATGG CAACATGGGG AGGTTCTACC 
CAGTGTGCAC TAGATGAAGA AGGAGATGAA GATGGTTACC TTTCTGAAGG AGTTGTTCGG 
ACAGATGAAG AGGAGGAAGA AGAAGAGCAA GATGCAAGTT CGAATGATAA CTTTTCTATG 
TATCCTCCTA ACAGTGCGAA TCATAACTCT TACAACATAA AAGAAACTAA GAATAGGTGG 
AAGAATAGTC GCCCTTTTAC AGCAGATGGA AATTATCGTC CTTTAGCCAA GACAAGACAG 
CAGAATATCA GCATGCAACG TCAGGAAAAC CTTCGTTGGA TGTCCGAACT CTCTTACGTA 
GAGGAGAAAG AACAATGGCA AGAACAAATT AATCAGCTGA AGAAACAACT TGATTTCAGT 
GTCAATATTT GTCAGACTTT GATGCAAGAC CAGCAGACGC TGTCTTGTCT GCTGCAAACT 
CTTCTCACAG GTCCTTACAG TGTTATGCCC AGCAATGTAG CTTCTCCTCA AGTACACCTT 
ATAATGCACC AGTTGAATCA GTGCTATACT CAGCTAACAT GGCAACAGAA TAATGTGCAA 
AGGTTGAAAC AAATGCTAAA TGAACTTATG CGCCAACAGA ATCAGCACCC AGAAAAACCT 
GGAAGCCAGG AAAGAGGCAG TAGTGCACCA CAGCCTTCTT CTCCCAGTTT ATTTTGTCCT 
TTCAGCTTTC CATCACAGCC TGTAAATCTA TTTAACCTAC CCGGGTTTAC TAATTTTTCA 
TCATTTGCAC CAGGTATGAA TTTTAGCCCT TTATTTCCTT CTAATTTTGG AGAATTTTCT 
CAGAATATTT CTACACCTAC TGAACAGCAG CAACCATTAG CCCAGAATTC TTCAGGGAAA 
ACAGAATATA TGGCTTTTCC AAAACCTTTT GAAAGCAGTT CTTCTATTGG AGCAGAAAAA 
CAAAGGAATC AAAAACAGCC TGGAGAGGAG GTGGAAAACA GTAGGACAGC GTGGTTATAT 
GACCAAGAAG GTGAAGTAGA AAAACCATTT ATCAAGACTG GATTTCCAGT GTCTGTAGAG 
AAAACTACAA ATAGTAATCG CAAAAATCAA TTAGATACCG GCCGAAGAAG ACGCCAGTTT 
GATGAAGAGT CATTAGAAAG CTTTAGCAGT ATGCCTGATC CAGTAGATCC GACAACAGTA 
ACCAAAACAT TCAAGACGAG AAAAGCATCT GCACAGGCCA GCCTAGCATC TAAAGATAAA 
ACTCCTAAAT CAAAGAGTAA GAAAAGGCAT TCTGCTCAAC TAAAAAGCAG AGTTAAAAAT 
ACTGGGTATG AAAGTGCCAG TGTGTCTAGC ACATGTGAAC CTTGCAAAAG TAGGAACAGA 
CATTCAGCCC AGACTGAAGA GCCTGTTCAA GCAAAAGTAT TCAGCAGAAA GAATCTTGAG 
CAGCTGGAAA AAATAATAAA ATATAGTAGG TCTACAGAGA TATCTTCAGA AACTGGGAGT 
GATTTTTCCA TGTTTGAAGC TTTGCGGGAT ACTATTTATT CTGAAGTAGC TACATTAATT 
TCTCAAAATG AATCTCATCC ACATTTTCTT ATTGAACTCT TCCATGAACT TCAGCTACTT 
AATACAGACT ACTTGAGACA AAGGGCTTTA TATGCATTGC AGGACATAGT AACCAGACAT 
ATTTCTGAGA ACCATGAAAA AGAAGGAGAA AATGTAAAGT CAGTGAATTC TGGTACTTGG 
ATAGCATCAA ACTCAGAGCT TACCCCCAGT GAAAGCCTTG CTACCACTGA TGATGAAACT 
TTTGAGAAGA ACTTTGAGAG AGAAACACAT AAAATAAGTG AGCAAAATGA TGCTGATAAT 
GCTAGCGTCA TGTCTGTATC TTCAAATTTT GAGCCTTTTG CAACAGATGA TCTAGGTAAC 
ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGGG AATACGAGCG TATGAAGACT 
GAGGCTGAAA GTAGCACAAA TATAAGATGC ACCTGCAGAA TTCTTGAGGA TGAGGATGGT 
GCTGCCGCCA CAAGTATGGT TACTAATTTA GAAGAAACTC CTATTGAAAA TCATGGTTCA 
CAACAACCTG TAAGTGAAGT TTCTACTGTT CCATGTCCTA GAATTGATAC TCAGCAGCTG 
GACCGGCAAA TTAAAGCCAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT 
GAAGTATGCT CTTCTCAACT TCTAACTTCA GTAAGGCGTA TGGTTTTGAC CCTCACCCAA 
CAAAATGATG AGAGCAAAGA ATTTGTGAAG TTCTTTCATA AACAACTTGG AAGTATATTA 
CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAAG ACTGTGGAGA AGATCTTCTT 
GTAGAGATAT CTGAAGTGTT GTTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG 
GATAATAATA GTATAACTGT TAAACAGAAG TGCAAAAGGA AAATAGAAGC AGCTGGAGTG 
ATACAGTCTT ATGCCAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCATGG CTCACCTGCT 
GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAAACTG AAACAGTTAA GCCGACTCAG 
ACATCTGAGA TATATGATGG TGATGGTCCC AAAAATGTGA GATCAGATGT TTCTGATCAA 
GAGGAAGATG AAGAAAGTGA AGAATGTCCG GTGTCTATTA ACTTGTCCAA AGCTGAAACT 
CAGGCTTTGA CTAATTATGG AAGTGGAGAA GATGAGAATG AGGATGAAGA AATAGAAGAA 
TTTGAAGAGG GACCTGTGGA TGTCCAGACG TCACTTCAGG CTAACACTGA AGCTACAGAA 
GAAACTGAAC ACGATGATCA GGTACTACAA CATGACTTTG AAAAATCAGG AGAAAGCAAA 
AATGTCCCAT CAGAACAAGA TCCCACTACT AGTAAAGGCA AGAAATATGA CCAAGATAGT 
ACTCCTGTGA AACCCTGTTA CCTCAATATC TTGGAAAATG AGCAGCCTTT GAATAGCGCT 
GTCCAAAAAG ACTCTCTGAC TACCATCGAT TCATCTAAAC AGCCTAACCC TTTGCCTTTA 
CCTTTACCTG AAATCGAAAC CTTGGTGCCT ACAGTCAAAG AAGTTAAATC TGCTCAAGAA 
ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC 
TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT 
GAAGATTTAC CACTTAAACT GACAATATAC TCAGAGGCAG ATCTAAGGAA GAAAATGGTA 
GAAGAGCAAG AGAAAAACCA TTTATCTGGT GAAATACTAT GTGAAATGCA GACCGAAGAA 
TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975620.1
CDS	615..6734
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLDCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_014120145.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120145.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GTTGTAACAG AAACTACAGG TAGCGTATCT GGAGTTAGTA TCACCTCTGA ACTAAATGAA 
GAATTGAATG ATTTAATTCA GCGTTTTCAT AATCAGCTTC GTGATTCTCA GCCTCCAACT 
GTTCCAGACA ATAGAAGACA AGCAGAAAGT CTTTCATTAA CTAGGGAGGT ATCCCAGAGC 
AGGAATCCAT CAGTTTCAGA ACACTTACCT GATGAGAAAG TACAACTGTT TAGCAAAATG 
AGAGTATTAC AGGAAAAGAA ACAAAAAATG GACAAACTAC TTGGAGAACT TCATACACTT 
CGAGATCAAC ATCTGAACAA TTCATCATTT GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT 
GTGGATCAGA GAAGTACAAC TTCAGCACCC TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC 
AATGGAGAAT CCAACAGCTT CACATCATCT GTTCCTTATC CTGTTGCTTC TCTAGTTTCT 
CAGAATGAGA GTGAAAACGA AGGACATCTA AATCCAACTG AAAAACTCCA GAAGTTAAAT 
GAAGTTCGAA AGAGATTGAA TGAACTAAGA GAACTAGTTC ATTATTACGA ACAAACATCA 
GATATGATGA CAGATGCTGT AAATGAAAAC ACAAAAGATG AAGAAACTGA AGAGTCAGAA 
TATGATTCTG AACATGAAAA CCCTGAACCT GTTACTAACA TTCGAAATCC TCAAGTAGCT 
GCCACTTGGA ATGAAGTAAA TAGTAATTCT AATGCACAGT GTGTTTCTAA TAATAGAGAG 
GGGAGATCAG TTAATTCTAA TTGTGAAATT AACAACAGAT CTGCTGCCAA CATAAGGACT 
CTGAACATGC CTCCTTCTTT AGATTGTCAC TATAATAGAG AAGGAGAACA GGGGATTCAT 
GGTGCACAAG GTGAAGACGA TGAGGAAGAA GAAGAAGCAG AAGATGAAGG AGTCAGTGGG 
GCTTCATTAA CTAGTCATAG GAGCAGTCTG GTTGATGAGG CTGCAGAAGA CGCTGAATTT 
GAACAGAAGA TCAATAGACT TATGGCTGCC AAACAGAAAT TAAGACAGTT ACAAGATCTT 
GTTGCTATGG TACAGGATGA TGATGCAGCA GATCATGGAG TTATCTCTGC CAATACTTCA 
AATTTGGATG ATTTTTACCC AGCAGAAGAA GACAACAAAC AAAGTGCAAA TAATACTAGA 
GGAAATGCCA ATAAAACACA GAAAGATGCT GGAATAAATG AGAAGGCAAG AGAGAAGTTT 
TATGAGGCTA AACTACAGCA GCAACAGAGA GAGCTCAGAC AATTGCAGGA AGAAAGAAAG 
AAACTGATTG AGATTCAAGA GAAAATTCAA GCATTACAGA AGGCATGCCC TGACTTACAG 
CTGTCAGCCA CTAGTGCGGG TAATTGTCCC ACAAAAAAAT ATATCCCAGC TGTTACTTCA 
ACCCCAGTTG TTAATGGGAA TGAAACCAGT ACAAGCAAAT CTGCTTTTGA GCCTGCAGAT 
CCTTCAGGAG TGGATAATGA GCTGTGGTCA GAAATGCGAA GACATGAGAT GTTAAGGGAA 
GAGCTACGGC AGAGAAGAAA GCAGCTTGAA GCACTGATGG CTGAACATCA GAGGAGGCAA 
GGTCTAGCTG AAACTACATC CCCTCTGGCT GTGTCATTGA GAAGTGATGG ATCTGAGAAC 
CTGTGTACGC CTCAACAGAG TAGAACAGAA AAAACCATGG CAACATGGGG AGGTTCTACC 
CAGTGTGCAC TAGATGAAGA AGGAGATGAA GATGGTTACC TTTCTGAAGG AGTTGTTCGG 
ACAGATGAAG AGGAGGAAGA AGAAGAGCAA GATGCAAGTT CGAATGATAA CTTTTCTATG 
TATCCTCCTA ACAGTGCGAA TCATAACTCT TACAACATAA AAGAAACTAA GAATAGGTGG 
AAGAATAGTC GCCCTTTTAC AGCAGATGGA AATTATCGTC CTTTAGCCAA GACAAGACAG 
CAGAATATCA GCATGCAACG TCAGGAAAAC CTTCGTTGGA TGTCCGAACT CTCTTACGTA 
GAGGAGAAAG AACAATGGCA AGAACAAATT AATCAGCTGA AGAAACAACT TGATTTCAGT 
GTCAATATTT GTCAGACTTT GATGCAAGAC CAGCAGACGC TGTCTTGTCT GCTGCAAACT 
CTTCTCACAG GTCCTTACAG TGTTATGCCC AGCAATGTAG CTTCTCCTCA AGTACACCTT 
ATAATGCACC AGTTGAATCA GTGCTATACT CAGCTAACAT GGCAACAGAA TAATGTGCAA 
AGGTTGAAAC AAATGCTAAA TGAACTTATG CGCCAACAGA ATCAGCACCC AGAAAAACCT 
GGAAGCCAGG AAAGAGGCAG TAGTGCACCA CAGCCTTCTT CTCCCAGTTT ATTTTGTCCT 
TTCAGCTTTC CATCACAGCC TGTAAATCTA TTTAACCTAC CCGGGTTTAC TAATTTTTCA 
TCATTTGCAC CAGGTATGAA TTTTAGCCCT TTATTTCCTT CTAATTTTGG AGAATTTTCT 
CAGAATATTT CTACACCTAC TGAACAGCAG CAACCATTAG CCCAGAATTC TTCAGGGAAA 
ACAGAATATA TGGCTTTTCC AAAACCTTTT GAAAGCAGTT CTTCTATTGG AGCAGAAAAA 
CAAAGGAATC AAAAACAGCC TGGAGAGGAG GTGGAAAACA GTAGGACAGC GTGGTTATAT 
GACCAAGAAG GTGAAGTAGA AAAACCATTT ATCAAGACTG GATTTCCAGT GTCTGTAGAG 
AAAACTACAA ATAGTAATCG CAAAAATCAA TTAGATACCG GCCGAAGAAG ACGCCAGTTT 
GATGAAGAGT CATTAGAAAG CTTTAGCAGT ATGCCTGATC CAGTAGATCC GACAACAGTA 
ACCAAAACAT TCAAGACGAG AAAAGCATCT GCACAGGCCA GCCTAGCATC TAAAGATAAA 
ACTCCTAAAT CAAAGAGTAA GAAAAGGCAT TCTGCTCAAC TAAAAAGCAG AGTTAAAAAT 
ACTGGGTATG AAAGTGCCAG TGTGTCTAGC ACATGTGAAC CTTGCAAAAG TAGGAACAGA 
CATTCAGCCC AGACTGAAGA GCCTGTTCAA GCAAAAGTAT TCAGCAGAAA GAATCTTGAG 
CAGCTGGAAA AAATAATAAA ATATAGTAGG TCTACAGAGA TATCTTCAGA AACTGGGAGT 
GATTTTTCCA TGTTTGAAGC TTTGCGGGAT ACTATTTATT CTGAAGTAGC TACATTAATT 
TCTCAAAATG AATCTCATCC ACATTTTCTT ATTGAACTCT TCCATGAACT TCAGCTACTT 
AATACAGACT ACTTGAGACA AAGGGCTTTA TATGCATTGC AGGACATAGT AACCAGACAT 
ATTTCTGAGA ACCATGAAAA AGAAGGAGAA AATGTAAAGT CAGTGAATTC TGGTACTTGG 
ATAGCATCAA ACTCAGAGCT TACCCCCAGT GAAAGCCTTG CTACCACTGA TGATGAAACT 
TTTGAGAAGA ACTTTGAGAG AGAAACACAT AAAATAAGTG AGCAAAATGA TGCTGATAAT 
GCTAGCGTCA TGTCTGTATC TTCAAATTTT GAGCCTTTTG CAACAGATGA TCTAGGTAAC 
ACTGTGATTC ACTTAGATCA GGCATTAGCC AGAATGAGGG AATACGAGCG TATGAAGACT 
GAGGCTGAAA GTAGCACAAA TATAAGATGC ACCTGCAGAA TTCTTGAGGA TGAGGATGGT 
GCTGCCGCCA CAAGTATGGT TACTAATTTA GAAGAAACTC CTATTGAAAA TCATGGTTCA 
CAACAACCTG TAAGTGAAGT TTCTACTGTT CCATGTCCTA GAATTGATAC TCAGCAGCTG 
GACCGGCAAA TTAAAGCCAT TATGAAAGAA GTCATTCCTT TTTTGAAGGA GCACATGGAT 
GAAGTATGCT CTTCTCAACT TCTAACTTCA GTAAGGCGTA TGGTTTTGAC CCTCACCCAA 
CAAAATGATG AGAGCAAAGA ATTTGTGAAG TTCTTTCATA AACAACTTGG AAGTATATTA 
CAGGATTCAC TGGCAAAATT TGCTGGCAGA AAACTGAAAG ACTGTGGAGA AGATCTTCTT 
GTAGAGATAT CTGAAGTGTT GTTTAATGAA TTGGCTTTCT TTAAGCTCAT GCAAGATTTG 
GATAATAATA GTATAACTGT TAAACAGAAG TGCAAAAGGA AAATAGAAGC AGCTGGAGTG 
ATACAGTCTT ATGCCAAAGA GGCCAAAAGG ATTCTTGAAG GAGATCATGG CTCACCTGCT 
GGAGAAATTG ATGATGAAGA CAAAGACAAG GATGAAACTG AAACAGTTAA GCCGACTCAG 
ACATCTGAGA TATATGATGG TGATGGTCCC AAAAATGTGA GATCAGATGT TTCTGATCAA 
GAGGAAGATG AAGAAAGTGA AGAATGTCCG GTGTCTATTA ACTTGTCCAA AGCTGAAACT 
CAGGCTTTGA CTAATTATGG AAGTGGAGAA GATGAGAATG AGGATGAAGA AATAGAAGAA 
TTTGAAGAGG GACCTGTGGA TGTCCAGACG TCACTTCAGG CTAACACTGA AGCTACAGAA 
GAAACTGAAC ACGATGATCA GGTACTACAA CATGACTTTG AAAAATCAGG AGAAAGCAAA 
AATGTCCCAT CAGAACAAGA TCCCACTACT AGTAAAGGCA AGAAATATGA CCAAGATAGT 
ACTCCTGTGA AACCCTGTTA CCTCAATATC TTGGAAAATG AGCAGCCTTT GAATAGCGCT 
GTCCAAAAAG ACTCTCTGAC TACCATCGAT TCATCTAAAC AGCCTAACCC TTTGCCTTTA 
CCTTTACCTG AAATCGAAAC CTTGGTGCCT ACAGTCAAAG AAGTTAAATC TGCTCAAGAA 
ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT GATACTGAAT CTCCAGTGTT AGTGAATGAC 
TATGAAGCAG AATCTGGTAA CATAAGTCAA AAATCTGATG AAGAAGACTT TGTGAAAGTT 
GAAGATTTAC CACTTAAACT GACAATATAC TCAGAGGCAG ATCTAAGGAA GAAAATGGTA 
GAAGAGCAAG AGAAAAACCA TTTATCTGGT GAAATACTAT GTGAAATGCA GACCGAAGAA 
TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA CCTGAAACAG TGGGAGCCCA AAGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629946.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630003.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X3, mRNA.

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CloneID	OCe18436
Clone ID Related Accession (Same CDS sequence)	XM_005629946.2 , XM_005629950.1 , XM_022404143.1 , XM_005629947.1 , XM_005629948.1 , XM_005629949.1 , XM_005629948.2 , XM_005629947.2 , XM_022404145.1 , XM_005629945.1 , XM_014120140.1 , XM_005629945.2 , XM_005629950.2
Accession Version	XM_005629946.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629946.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630003.1
CDS	774..7349
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629946.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629946.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629950.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630007.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X7, mRNA.

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CloneID	OCe18436
Clone ID Related Accession (Same CDS sequence)	XM_005629946.2 , XM_005629950.1 , XM_022404143.1 , XM_005629947.1 , XM_005629948.1 , XM_005629949.1 , XM_005629948.2 , XM_005629947.2 , XM_022404145.1 , XM_005629945.1 , XM_014120140.1 , XM_005629945.2 , XM_005629950.2
Accession Version	XM_005629950.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630007.1
CDS	512..7087
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629950.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629950.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404143.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259851.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

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CloneID	OCe18436
Clone ID Related Accession (Same CDS sequence)	XM_005629946.2 , XM_005629950.1 , XM_022404143.1 , XM_005629947.1 , XM_005629948.1 , XM_005629949.1 , XM_005629948.2 , XM_005629947.2 , XM_022404145.1 , XM_005629945.1 , XM_014120140.1 , XM_005629945.2 , XM_005629950.2
Accession Version	XM_022404143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259851.1
CDS	422..6997
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404143.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404143.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629947.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630004.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X4, mRNA.

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CloneID	OCe18436
Clone ID Related Accession (Same CDS sequence)	XM_005629946.2 , XM_005629950.1 , XM_022404143.1 , XM_005629947.1 , XM_005629948.1 , XM_005629949.1 , XM_005629948.2 , XM_005629947.2 , XM_022404145.1 , XM_005629945.1 , XM_014120140.1 , XM_005629945.2 , XM_005629950.2
Accession Version	XM_005629947.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630004.1
CDS	585..7160
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629947.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629947.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629948.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630005.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

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CloneID	OCe18436
Clone ID Related Accession (Same CDS sequence)	XM_005629946.2 , XM_005629950.1 , XM_022404143.1 , XM_005629947.1 , XM_005629948.1 , XM_005629949.1 , XM_005629948.2 , XM_005629947.2 , XM_022404145.1 , XM_005629945.1 , XM_014120140.1 , XM_005629945.2 , XM_005629950.2
Accession Version	XM_005629948.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630005.1
CDS	437..7012
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629948.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629948.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629949.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630006.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X6, mRNA.

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CloneID	OCe18436
Clone ID Related Accession (Same CDS sequence)	XM_005629946.2 , XM_005629950.1 , XM_022404143.1 , XM_005629947.1 , XM_005629948.1 , XM_005629949.1 , XM_005629948.2 , XM_005629947.2 , XM_022404145.1 , XM_005629945.1 , XM_014120140.1 , XM_005629945.2 , XM_005629950.2
Accession Version	XM_005629949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630006.1
CDS	543..7118
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629949.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629949.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629948.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630005.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

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CloneID	OCe18436
Clone ID Related Accession (Same CDS sequence)	XM_005629946.2 , XM_005629950.1 , XM_022404143.1 , XM_005629947.1 , XM_005629948.1 , XM_005629949.1 , XM_005629948.2 , XM_005629947.2 , XM_022404145.1 , XM_005629945.1 , XM_014120140.1 , XM_005629945.2 , XM_005629950.2
Accession Version	XM_005629948.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629948.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630005.1
CDS	426..7001
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629948.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629948.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629947.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630004.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X4, mRNA.

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CloneID	OCe18436
Clone ID Related Accession (Same CDS sequence)	XM_005629946.2 , XM_005629950.1 , XM_022404143.1 , XM_005629947.1 , XM_005629948.1 , XM_005629949.1 , XM_005629948.2 , XM_005629947.2 , XM_022404145.1 , XM_005629945.1 , XM_014120140.1 , XM_005629945.2 , XM_005629950.2
Accession Version	XM_005629947.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629947.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630004.1
CDS	574..7149
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629947.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629947.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404145.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259853.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

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CloneID	OCe18436
Clone ID Related Accession (Same CDS sequence)	XM_005629946.2 , XM_005629950.1 , XM_022404143.1 , XM_005629947.1 , XM_005629948.1 , XM_005629949.1 , XM_005629948.2 , XM_005629947.2 , XM_022404145.1 , XM_005629945.1 , XM_014120140.1 , XM_005629945.2 , XM_005629950.2
Accession Version	XM_022404145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259853.1
CDS	350..6925
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404145.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404145.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629945.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630002.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

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CloneID	OCe18436
Clone ID Related Accession (Same CDS sequence)	XM_005629946.2 , XM_005629950.1 , XM_022404143.1 , XM_005629947.1 , XM_005629948.1 , XM_005629949.1 , XM_005629948.2 , XM_005629947.2 , XM_022404145.1 , XM_005629945.1 , XM_014120140.1 , XM_005629945.2 , XM_005629950.2
Accession Version	XM_005629945.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630002.1
CDS	616..7191
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629945.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629945.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_014120140.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975615.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

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CloneID	OCe18436
Clone ID Related Accession (Same CDS sequence)	XM_005629946.2 , XM_005629950.1 , XM_022404143.1 , XM_005629947.1 , XM_005629948.1 , XM_005629949.1 , XM_005629948.2 , XM_005629947.2 , XM_022404145.1 , XM_005629945.1 , XM_014120140.1 , XM_005629945.2 , XM_005629950.2
Accession Version	XM_014120140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975615.1
CDS	613..7188
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_014120140.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120140.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629945.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630002.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X3, mRNA.

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CloneID	OCe18436
Clone ID Related Accession (Same CDS sequence)	XM_005629946.2 , XM_005629950.1 , XM_022404143.1 , XM_005629947.1 , XM_005629948.1 , XM_005629949.1 , XM_005629948.2 , XM_005629947.2 , XM_022404145.1 , XM_005629945.1 , XM_014120140.1 , XM_005629945.2 , XM_005629950.2
Accession Version	XM_005629945.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629945.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630002.1
CDS	605..7180
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629945.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629945.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629950.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630007.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X6, mRNA.

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CloneID	OCe18436
Clone ID Related Accession (Same CDS sequence)	XM_005629946.2 , XM_005629950.1 , XM_022404143.1 , XM_005629947.1 , XM_005629948.1 , XM_005629949.1 , XM_005629948.2 , XM_005629947.2 , XM_022404145.1 , XM_005629945.1 , XM_014120140.1 , XM_005629945.2 , XM_005629950.2
Accession Version	XM_005629950.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629950.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630007.1
CDS	498..7073
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629950.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629950.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGGCAAGAA ATATGACCAA GATAGTACTC CTGTGAAACC CTGTTACCTC 
AATATCTTGG AAAATGAGCA GCCTTTGAAT AGCGCTGTCC AAAAAGACTC TCTGACTACC 
ATCGATTCAT CTAAACAGCC TAACCCTTTG CCTTTACCTT TACCTGAAAT CGAAACCTTG 
GTGCCTACAG TCAAAGAAGT TAAATCTGCT CAAGAAACTC CTGAAAGCTC TCTGGCTGGA 
AGTCCTGATA CTGAATCTCC AGTGTTAGTG AATGACTATG AAGCAGAATC TGGTAACATA 
AGTCAAAAAT CTGATGAAGA AGACTTTGTG AAAGTTGAAG ATTTACCACT TAAACTGACA 
ATATACTCAG AGGCAGATCT AAGGAAGAAA ATGGTAGAAG AGCAAGAGAA AAACCATTTA 
TCTGGTGAAA TACTATGTGA AATGCAGACC GAAGAATTAG CTGGGAATTC TCAGACACTA 
AAAGAACCTG AAACAGTGGG AGCCCAAAGC GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629951.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630008.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X9, mRNA.

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CloneID	OCe18437
Clone ID Related Accession (Same CDS sequence)	XM_005629951.1 , XM_022404147.1
Accession Version	XM_005629951.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGATGACCA AGATAGTACT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAAATGAGC AGCCTTTGAA TAGCGCTGTC CAAAAAGACT CTCTGACTAC CATCGATTCA 
TCTAAACAGC CTAACCCTTT GCCTTTACCT TTACCTGAAA TCGAAACCTT GGTGCCTACA 
GTCAAAGAAG TTAAATCTGC TCAAGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAACAT AAGTCAAAAA 
TCTGATGAAG AAGACTTTGT GAAAGTTGAA GATTTACCAC TTAAACTGAC AATATACTCA 
GAGGCAGATC TAAGGAAGAA AATGGTAGAA GAGCAAGAGA AAAACCATTT ATCTGGTGAA 
ATACTATGTG AAATGCAGAC CGAAGAATTA GCTGGGAATT CTCAGACACT AAAAGAACCT 
GAAACAGTGG GAGCCCAAAG CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630008.1
CDS	616..7182
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKDDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629951.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629951.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGATGACCA AGATAGTACT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAAATGAGC AGCCTTTGAA TAGCGCTGTC CAAAAAGACT CTCTGACTAC CATCGATTCA 
TCTAAACAGC CTAACCCTTT GCCTTTACCT TTACCTGAAA TCGAAACCTT GGTGCCTACA 
GTCAAAGAAG TTAAATCTGC TCAAGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAACAT AAGTCAAAAA 
TCTGATGAAG AAGACTTTGT GAAAGTTGAA GATTTACCAC TTAAACTGAC AATATACTCA 
GAGGCAGATC TAAGGAAGAA AATGGTAGAA GAGCAAGAGA AAAACCATTT ATCTGGTGAA 
ATACTATGTG AAATGCAGAC CGAAGAATTA GCTGGGAATT CTCAGACACT AAAAGAACCT 
GAAACAGTGG GAGCCCAAAG CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404147.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259855.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X8, mRNA.

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CloneID	OCe18437
Clone ID Related Accession (Same CDS sequence)	XM_005629951.1 , XM_022404147.1
Accession Version	XM_022404147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGATGACCA AGATAGTACT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAAATGAGC AGCCTTTGAA TAGCGCTGTC CAAAAAGACT CTCTGACTAC CATCGATTCA 
TCTAAACAGC CTAACCCTTT GCCTTTACCT TTACCTGAAA TCGAAACCTT GGTGCCTACA 
GTCAAAGAAG TTAAATCTGC TCAAGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAACAT AAGTCAAAAA 
TCTGATGAAG AAGACTTTGT GAAAGTTGAA GATTTACCAC TTAAACTGAC AATATACTCA 
GAGGCAGATC TAAGGAAGAA AATGGTAGAA GAGCAAGAGA AAAACCATTT ATCTGGTGAA 
ATACTATGTG AAATGCAGAC CGAAGAATTA GCTGGGAATT CTCAGACACT AAAAGAACCT 
GAAACAGTGG GAGCCCAAAG CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259855.1
CDS	426..6992
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKDDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404147.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404147.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGCA CATGCTAGGA GAATACTACA GCAGTCTAAC 
AGAAATGCAT GCAATGAAGC ACCAGAAACT GGGAGTGATT TTTCCATGTT TGAAGCTTTG 
CGGGATACTA TTTATTCTGA AGTAGCTACA TTAATTTCTC AAAATGAATC TCATCCACAT 
TTTCTTATTG AACTCTTCCA TGAACTTCAG CTACTTAATA CAGACTACTT GAGACAAAGG 
GCTTTATATG CATTGCAGGA CATAGTAACC AGACATATTT CTGAGAACCA TGAAAAAGAA 
GGAGAAAATG TAAAGTCAGT GAATTCTGGT ACTTGGATAG CATCAAACTC AGAGCTTACC 
CCCAGTGAAA GCCTTGCTAC CACTGATGAT GAAACTTTTG AGAAGAACTT TGAGAGAGAA 
ACACATAAAA TAAGTGAGCA AAATGATGCT GATAATGCTA GCGTCATGTC TGTATCTTCA 
AATTTTGAGC CTTTTGCAAC AGATGATCTA GGTAACACTG TGATTCACTT AGATCAGGCA 
TTAGCCAGAA TGAGGGAATA CGAGCGTATG AAGACTGAGG CTGAAAGTAG CACAAATATA 
AGATGCACCT GCAGAATTCT TGAGGATGAG GATGGTGCTG CCGCCACAAG TATGGTTACT 
AATTTAGAAG AAACTCCTAT TGAAAATCAT GGTTCACAAC AACCTGTAAG TGAAGTTTCT 
ACTGTTCCAT GTCCTAGAAT TGATACTCAG CAGCTGGACC GGCAAATTAA AGCCATTATG 
AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC ATGGATGAAG TATGCTCTTC TCAACTTCTA 
ACTTCAGTAA GGCGTATGGT TTTGACCCTC ACCCAACAAA ATGATGAGAG CAAAGAATTT 
GTGAAGTTCT TTCATAAACA ACTTGGAAGT ATATTACAGG ATTCACTGGC AAAATTTGCT 
GGCAGAAAAC TGAAAGACTG TGGAGAAGAT CTTCTTGTAG AGATATCTGA AGTGTTGTTT 
AATGAATTGG CTTTCTTTAA GCTCATGCAA GATTTGGATA ATAATAGTAT AACTGTTAAA 
CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT GGAGTGATAC AGTCTTATGC CAAAGAGGCC 
AAAAGGATTC TTGAAGGAGA TCATGGCTCA CCTGCTGGAG AAATTGATGA TGAAGACAAA 
GACAAGGATG AAACTGAAAC AGTTAAGCCG ACTCAGACAT CTGAGATATA TGATGGTGAT 
GGTCCCAAAA ATGTGAGATC AGATGTTTCT GATCAAGAGG AAGATGAAGA AAGTGAAGAA 
TGTCCGGTGT CTATTAACTT GTCCAAAGCT GAAACTCAGG CTTTGACTAA TTATGGAAGT 
GGAGAAGATG AGAATGAGGA TGAAGAAATA GAAGAATTTG AAGAGGGACC TGTGGATGTC 
CAGACGTCAC TTCAGGCTAA CACTGAAGCT ACAGAAGAAA CTGAACACGA TGATCAGGTA 
CTACAACATG ACTTTGAAAA ATCAGGAGAA AGCAAAAATG TCCCATCAGA ACAAGATCCC 
ACTACTAGTA AAGATGACCA AGATAGTACT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAAATGAGC AGCCTTTGAA TAGCGCTGTC CAAAAAGACT CTCTGACTAC CATCGATTCA 
TCTAAACAGC CTAACCCTTT GCCTTTACCT TTACCTGAAA TCGAAACCTT GGTGCCTACA 
GTCAAAGAAG TTAAATCTGC TCAAGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAACAT AAGTCAAAAA 
TCTGATGAAG AAGACTTTGT GAAAGTTGAA GATTTACCAC TTAAACTGAC AATATACTCA 
GAGGCAGATC TAAGGAAGAA AATGGTAGAA GAGCAAGAGA AAAACCATTT ATCTGGTGAA 
ATACTATGTG AAATGCAGAC CGAAGAATTA GCTGGGAATT CTCAGACACT AAAAGAACCT 
GAAACAGTGG GAGCCCAAAG CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629952.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630009.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X10, mRNA.

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CloneID	OCe18438
Clone ID Related Accession (Same CDS sequence)	XM_005629952.2 , XM_022404148.1
Accession Version	XM_005629952.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGGGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCATCCA 
CATTTTCTTA TTGAACTCTT CCATGAACTT CAGCTACTTA ATACAGACTA CTTGAGACAA 
AGGGCTTTAT ATGCATTGCA GGACATAGTA ACCAGACATA TTTCTGAGAA CCATGAAAAA 
GAAGGAGAAA ATGTAAAGTC AGTGAATTCT GGTACTTGGA TAGCATCAAA CTCAGAGCTT 
ACCCCCAGTG AAAGCCTTGC TACCACTGAT GATGAAACTT TTGAGAAGAA CTTTGAGAGA 
GAAACACATA AAATAAGTGA GCAAAATGAT GCTGATAATG CTAGCGTCAT GTCTGTATCT 
TCAAATTTTG AGCCTTTTGC AACAGATGAT CTAGGTAACA CTGTGATTCA CTTAGATCAG 
GCATTAGCCA GAATGAGGGA ATACGAGCGT ATGAAGACTG AGGCTGAAAG TAGCACAAAT 
ATAAGATGCA CCTGCAGAAT TCTTGAGGAT GAGGATGGTG CTGCCGCCAC AAGTATGGTT 
ACTAATTTAG AAGAAACTCC TATTGAAAAT CATGGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACTGTTC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCCATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC TTCTCAACTT 
CTAACTTCAG TAAGGCGTAT GGTTTTGACC CTCACCCAAC AAAATGATGA GAGCAAAGAA 
TTTGTGAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTTAATGAAT TGGCTTTCTT TAAGCTCATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAAGT GCAAAAGGAA AATAGAAGCA GCTGGAGTGA TACAGTCTTA TGCCAAAGAG 
GCCAAAAGGA TTCTTGAAGG AGATCATGGC TCACCTGCTG GAGAAATTGA TGATGAAGAC 
AAAGACAAGG ATGAAACTGA AACAGTTAAG CCGACTCAGA CATCTGAGAT ATATGATGGT 
GATGGTCCCA AAAATGTGAG ATCAGATGTT TCTGATCAAG AGGAAGATGA AGAAAGTGAA 
GAATGTCCGG TGTCTATTAA CTTGTCCAAA GCTGAAACTC AGGCTTTGAC TAATTATGGA 
AGTGGAGAAG ATGAGAATGA GGATGAAGAA ATAGAAGAAT TTGAAGAGGG ACCTGTGGAT 
GTCCAGACGT CACTTCAGGC TAACACTGAA GCTACAGAAG AAACTGAACA CGATGATCAG 
GTACTACAAC ATGACTTTGA AAAATCAGGA GAAAGCAAAA ATGTCCCATC AGAACAAGAT 
CCCACTACTA GTAAAGGCAA GAAATATGAC CAAGATAGTA CTCCTGTGAA ACCCTGTTAC 
CTCAATATCT TGGAAAATGA GCAGCCTTTG AATAGCGCTG TCCAAAAAGA CTCTCTGACT 
ACCATCGATT CATCTAAACA GCCTAACCCT TTGCCTTTAC CTTTACCTGA AATCGAAACC 
TTGGTGCCTA CAGTCAAAGA AGTTAAATCT GCTCAAGAAA CTCCTGAAAG CTCTCTGGCT 
GGAAGTCCTG ATACTGAATC TCCAGTGTTA GTGAATGACT ATGAAGCAGA ATCTGGTAAC 
ATAAGTCAAA AATCTGATGA AGAAGACTTT GTGAAAGTTG AAGATTTACC ACTTAAACTG 
ACAATATACT CAGAGGCAGA TCTAAGGAAG AAAATGGTAG AAGAGCAAGA GAAAAACCAT 
TTATCTGGTG AAATACTATG TGAAATGCAG ACCGAAGAAT TAGCTGGGAA TTCTCAGACA 
CTAAAAGAAC CTGAAACAGT GGGAGCCCAA AGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630009.1
CDS	617..7135
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629952.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629952.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGGGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCATCCA 
CATTTTCTTA TTGAACTCTT CCATGAACTT CAGCTACTTA ATACAGACTA CTTGAGACAA 
AGGGCTTTAT ATGCATTGCA GGACATAGTA ACCAGACATA TTTCTGAGAA CCATGAAAAA 
GAAGGAGAAA ATGTAAAGTC AGTGAATTCT GGTACTTGGA TAGCATCAAA CTCAGAGCTT 
ACCCCCAGTG AAAGCCTTGC TACCACTGAT GATGAAACTT TTGAGAAGAA CTTTGAGAGA 
GAAACACATA AAATAAGTGA GCAAAATGAT GCTGATAATG CTAGCGTCAT GTCTGTATCT 
TCAAATTTTG AGCCTTTTGC AACAGATGAT CTAGGTAACA CTGTGATTCA CTTAGATCAG 
GCATTAGCCA GAATGAGGGA ATACGAGCGT ATGAAGACTG AGGCTGAAAG TAGCACAAAT 
ATAAGATGCA CCTGCAGAAT TCTTGAGGAT GAGGATGGTG CTGCCGCCAC AAGTATGGTT 
ACTAATTTAG AAGAAACTCC TATTGAAAAT CATGGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACTGTTC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCCATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC TTCTCAACTT 
CTAACTTCAG TAAGGCGTAT GGTTTTGACC CTCACCCAAC AAAATGATGA GAGCAAAGAA 
TTTGTGAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTTAATGAAT TGGCTTTCTT TAAGCTCATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAAGT GCAAAAGGAA AATAGAAGCA GCTGGAGTGA TACAGTCTTA TGCCAAAGAG 
GCCAAAAGGA TTCTTGAAGG AGATCATGGC TCACCTGCTG GAGAAATTGA TGATGAAGAC 
AAAGACAAGG ATGAAACTGA AACAGTTAAG CCGACTCAGA CATCTGAGAT ATATGATGGT 
GATGGTCCCA AAAATGTGAG ATCAGATGTT TCTGATCAAG AGGAAGATGA AGAAAGTGAA 
GAATGTCCGG TGTCTATTAA CTTGTCCAAA GCTGAAACTC AGGCTTTGAC TAATTATGGA 
AGTGGAGAAG ATGAGAATGA GGATGAAGAA ATAGAAGAAT TTGAAGAGGG ACCTGTGGAT 
GTCCAGACGT CACTTCAGGC TAACACTGAA GCTACAGAAG AAACTGAACA CGATGATCAG 
GTACTACAAC ATGACTTTGA AAAATCAGGA GAAAGCAAAA ATGTCCCATC AGAACAAGAT 
CCCACTACTA GTAAAGGCAA GAAATATGAC CAAGATAGTA CTCCTGTGAA ACCCTGTTAC 
CTCAATATCT TGGAAAATGA GCAGCCTTTG AATAGCGCTG TCCAAAAAGA CTCTCTGACT 
ACCATCGATT CATCTAAACA GCCTAACCCT TTGCCTTTAC CTTTACCTGA AATCGAAACC 
TTGGTGCCTA CAGTCAAAGA AGTTAAATCT GCTCAAGAAA CTCCTGAAAG CTCTCTGGCT 
GGAAGTCCTG ATACTGAATC TCCAGTGTTA GTGAATGACT ATGAAGCAGA ATCTGGTAAC 
ATAAGTCAAA AATCTGATGA AGAAGACTTT GTGAAAGTTG AAGATTTACC ACTTAAACTG 
ACAATATACT CAGAGGCAGA TCTAAGGAAG AAAATGGTAG AAGAGCAAGA GAAAAACCAT 
TTATCTGGTG AAATACTATG TGAAATGCAG ACCGAAGAAT TAGCTGGGAA TTCTCAGACA 
CTAAAAGAAC CTGAAACAGT GGGAGCCCAA AGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404148.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259856.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X9, mRNA.

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CloneID	OCe18438
Clone ID Related Accession (Same CDS sequence)	XM_005629952.2 , XM_022404148.1
Accession Version	XM_022404148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGGGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCATCCA 
CATTTTCTTA TTGAACTCTT CCATGAACTT CAGCTACTTA ATACAGACTA CTTGAGACAA 
AGGGCTTTAT ATGCATTGCA GGACATAGTA ACCAGACATA TTTCTGAGAA CCATGAAAAA 
GAAGGAGAAA ATGTAAAGTC AGTGAATTCT GGTACTTGGA TAGCATCAAA CTCAGAGCTT 
ACCCCCAGTG AAAGCCTTGC TACCACTGAT GATGAAACTT TTGAGAAGAA CTTTGAGAGA 
GAAACACATA AAATAAGTGA GCAAAATGAT GCTGATAATG CTAGCGTCAT GTCTGTATCT 
TCAAATTTTG AGCCTTTTGC AACAGATGAT CTAGGTAACA CTGTGATTCA CTTAGATCAG 
GCATTAGCCA GAATGAGGGA ATACGAGCGT ATGAAGACTG AGGCTGAAAG TAGCACAAAT 
ATAAGATGCA CCTGCAGAAT TCTTGAGGAT GAGGATGGTG CTGCCGCCAC AAGTATGGTT 
ACTAATTTAG AAGAAACTCC TATTGAAAAT CATGGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACTGTTC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCCATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC TTCTCAACTT 
CTAACTTCAG TAAGGCGTAT GGTTTTGACC CTCACCCAAC AAAATGATGA GAGCAAAGAA 
TTTGTGAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTTAATGAAT TGGCTTTCTT TAAGCTCATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAAGT GCAAAAGGAA AATAGAAGCA GCTGGAGTGA TACAGTCTTA TGCCAAAGAG 
GCCAAAAGGA TTCTTGAAGG AGATCATGGC TCACCTGCTG GAGAAATTGA TGATGAAGAC 
AAAGACAAGG ATGAAACTGA AACAGTTAAG CCGACTCAGA CATCTGAGAT ATATGATGGT 
GATGGTCCCA AAAATGTGAG ATCAGATGTT TCTGATCAAG AGGAAGATGA AGAAAGTGAA 
GAATGTCCGG TGTCTATTAA CTTGTCCAAA GCTGAAACTC AGGCTTTGAC TAATTATGGA 
AGTGGAGAAG ATGAGAATGA GGATGAAGAA ATAGAAGAAT TTGAAGAGGG ACCTGTGGAT 
GTCCAGACGT CACTTCAGGC TAACACTGAA GCTACAGAAG AAACTGAACA CGATGATCAG 
GTACTACAAC ATGACTTTGA AAAATCAGGA GAAAGCAAAA ATGTCCCATC AGAACAAGAT 
CCCACTACTA GTAAAGGCAA GAAATATGAC CAAGATAGTA CTCCTGTGAA ACCCTGTTAC 
CTCAATATCT TGGAAAATGA GCAGCCTTTG AATAGCGCTG TCCAAAAAGA CTCTCTGACT 
ACCATCGATT CATCTAAACA GCCTAACCCT TTGCCTTTAC CTTTACCTGA AATCGAAACC 
TTGGTGCCTA CAGTCAAAGA AGTTAAATCT GCTCAAGAAA CTCCTGAAAG CTCTCTGGCT 
GGAAGTCCTG ATACTGAATC TCCAGTGTTA GTGAATGACT ATGAAGCAGA ATCTGGTAAC 
ATAAGTCAAA AATCTGATGA AGAAGACTTT GTGAAAGTTG AAGATTTACC ACTTAAACTG 
ACAATATACT CAGAGGCAGA TCTAAGGAAG AAAATGGTAG AAGAGCAAGA GAAAAACCAT 
TTATCTGGTG AAATACTATG TGAAATGCAG ACCGAAGAAT TAGCTGGGAA TTCTCAGACA 
CTAAAAGAAC CTGAAACAGT GGGAGCCCAA AGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259856.1
CDS	426..6944
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404148.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404148.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGGGTATGA AAGTGCCAGT 
GTGTCTAGCA CATGTGAACC TTGCAAAAGT AGGAACAGAC ATTCAGCCCA GACTGAAGAG 
CCTGTTCAAG CAAAAGTATT CAGCAGAAAG AATCTTGAGC AGCTGGAAAA AATAATAAAA 
TATAGTAGGT CTACAGAGAT ATCTTCAGAA ACTGGGAGTG ATTTTTCCAT GTTTGAAGCT 
TTGCGGGATA CTATTTATTC TGAAGTAGCT ACATTAATTT CTCAAAATGA ATCTCATCCA 
CATTTTCTTA TTGAACTCTT CCATGAACTT CAGCTACTTA ATACAGACTA CTTGAGACAA 
AGGGCTTTAT ATGCATTGCA GGACATAGTA ACCAGACATA TTTCTGAGAA CCATGAAAAA 
GAAGGAGAAA ATGTAAAGTC AGTGAATTCT GGTACTTGGA TAGCATCAAA CTCAGAGCTT 
ACCCCCAGTG AAAGCCTTGC TACCACTGAT GATGAAACTT TTGAGAAGAA CTTTGAGAGA 
GAAACACATA AAATAAGTGA GCAAAATGAT GCTGATAATG CTAGCGTCAT GTCTGTATCT 
TCAAATTTTG AGCCTTTTGC AACAGATGAT CTAGGTAACA CTGTGATTCA CTTAGATCAG 
GCATTAGCCA GAATGAGGGA ATACGAGCGT ATGAAGACTG AGGCTGAAAG TAGCACAAAT 
ATAAGATGCA CCTGCAGAAT TCTTGAGGAT GAGGATGGTG CTGCCGCCAC AAGTATGGTT 
ACTAATTTAG AAGAAACTCC TATTGAAAAT CATGGTTCAC AACAACCTGT AAGTGAAGTT 
TCTACTGTTC CATGTCCTAG AATTGATACT CAGCAGCTGG ACCGGCAAAT TAAAGCCATT 
ATGAAAGAAG TCATTCCTTT TTTGAAGGAG CACATGGATG AAGTATGCTC TTCTCAACTT 
CTAACTTCAG TAAGGCGTAT GGTTTTGACC CTCACCCAAC AAAATGATGA GAGCAAAGAA 
TTTGTGAAGT TCTTTCATAA ACAACTTGGA AGTATATTAC AGGATTCACT GGCAAAATTT 
GCTGGCAGAA AACTGAAAGA CTGTGGAGAA GATCTTCTTG TAGAGATATC TGAAGTGTTG 
TTTAATGAAT TGGCTTTCTT TAAGCTCATG CAAGATTTGG ATAATAATAG TATAACTGTT 
AAACAGAAGT GCAAAAGGAA AATAGAAGCA GCTGGAGTGA TACAGTCTTA TGCCAAAGAG 
GCCAAAAGGA TTCTTGAAGG AGATCATGGC TCACCTGCTG GAGAAATTGA TGATGAAGAC 
AAAGACAAGG ATGAAACTGA AACAGTTAAG CCGACTCAGA CATCTGAGAT ATATGATGGT 
GATGGTCCCA AAAATGTGAG ATCAGATGTT TCTGATCAAG AGGAAGATGA AGAAAGTGAA 
GAATGTCCGG TGTCTATTAA CTTGTCCAAA GCTGAAACTC AGGCTTTGAC TAATTATGGA 
AGTGGAGAAG ATGAGAATGA GGATGAAGAA ATAGAAGAAT TTGAAGAGGG ACCTGTGGAT 
GTCCAGACGT CACTTCAGGC TAACACTGAA GCTACAGAAG AAACTGAACA CGATGATCAG 
GTACTACAAC ATGACTTTGA AAAATCAGGA GAAAGCAAAA ATGTCCCATC AGAACAAGAT 
CCCACTACTA GTAAAGGCAA GAAATATGAC CAAGATAGTA CTCCTGTGAA ACCCTGTTAC 
CTCAATATCT TGGAAAATGA GCAGCCTTTG AATAGCGCTG TCCAAAAAGA CTCTCTGACT 
ACCATCGATT CATCTAAACA GCCTAACCCT TTGCCTTTAC CTTTACCTGA AATCGAAACC 
TTGGTGCCTA CAGTCAAAGA AGTTAAATCT GCTCAAGAAA CTCCTGAAAG CTCTCTGGCT 
GGAAGTCCTG ATACTGAATC TCCAGTGTTA GTGAATGACT ATGAAGCAGA ATCTGGTAAC 
ATAAGTCAAA AATCTGATGA AGAAGACTTT GTGAAAGTTG AAGATTTACC ACTTAAACTG 
ACAATATACT CAGAGGCAGA TCTAAGGAAG AAAATGGTAG AAGAGCAAGA GAAAAACCAT 
TTATCTGGTG AAATACTATG TGAAATGCAG ACCGAAGAAT TAGCTGGGAA TTCTCAGACA 
CTAAAAGAAC CTGAAACAGT GGGAGCCCAA AGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629953.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630010.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X12, mRNA.

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CloneID	OCe18439
Clone ID Related Accession (Same CDS sequence)	XM_005629953.1 , XM_022404150.1
Accession Version	XM_005629953.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGTTG TAACAGAAAC TACAGGTAGC GTATCTGGAG TTAGTATCAC CTCTGAACTA 
AATGAAGAAT TGAATGATTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAACTGTTC CAGACAATAG AAGACAAGCA GAAAGTCTTT CATTAACTAG GGAGGTATCC 
CAGAGCAGGA ATCCATCAGT TTCAGAACAC TTACCTGATG AGAAAGTACA ACTGTTTAGC 
AAAATGAGAG TATTACAGGA AAAGAAACAA AAAATGGACA AACTACTTGG AGAACTTCAT 
ACACTTCGAG ATCAACATCT GAACAATTCA TCATTTGTGC CTTCTTCAGC TTCTCCACAA 
AGAAGTGTGG ATCAGAGAAG TACAACTTCA GCACCCTCTG CTCCTATAGG CTTAGCACCA 
GTTGTCAATG GAGAATCCAA CAGCTTCACA TCATCTGTTC CTTATCCTGT TGCTTCTCTA 
GTTTCTCAGA ATGAGAGTGA AAACGAAGGA CATCTAAATC CAACTGAAAA ACTCCAGAAG 
TTAAATGAAG TTCGAAAGAG ATTGAATGAA CTAAGAGAAC TAGTTCATTA TTACGAACAA 
ACATCAGATA TGATGACAGA TGCTGTAAAT GAAAACACAA AAGATGAAGA AACTGAAGAG 
TCAGAATATG ATTCTGAACA TGAAAACCCT GAACCTGTTA CTAACATTCG AAATCCTCAA 
GTAGCTGCCA CTTGGAATGA AGTAAATAGT AATTCTAATG CACAGTGTGT TTCTAATAAT 
AGAGAGGGGA GATCAGTTAA TTCTAATTGT GAAATTAACA ACAGATCTGC TGCCAACATA 
AGGACTCTGA ACATGCCTCC TTCTTTAGCA GATTGTCACT ATAATAGAGA AGGAGAACAG 
GGGATTCATG GTGCACAAGG TGAAGACGAT GAGGAAGAAG AAGAAGCAGA AGATGAAGGA 
GTCAGTGGGG CTTCATTAAC TAGTCATAGG AGCAGTCTGG TTGATGAGGC TGCAGAAGAC 
GCTGAATTTG AACAGAAGAT CAATAGACTT ATGGCTGCCA AACAGAAATT AAGACAGTTA 
CAAGATCTTG TTGCTATGGT ACAGGATGAT GATGCAGCAG ATCATGGAGT TATCTCTGCC 
AATACTTCAA ATTTGGATGA TTTTTACCCA GCAGAAGAAG ACAACAAACA AAGTGCAAAT 
AATACTAGAG GAAATGCCAA TAAAACACAG AAAGATGCTG GAATAAATGA GAAGGCAAGA 
GAGAAGTTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AGCTCAGACA ATTGCAGGAA 
GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG CATTACAGAA GGCATGCCCT 
GACTTACAGC TGTCAGCCAC TAGTGCGGGT AATTGTCCCA CAAAAAAATA TATCCCAGCT 
GTTACTTCAA CCCCAGTTGT TAATGGGAAT GAAACCAGTA CAAGCAAATC TGCTTTTGAG 
CCTGCAGATC CTTCAGGAGT GGATAATGAG CTGTGGTCAG AAATGCGAAG ACATGAGATG 
TTAAGGGAAG AGCTACGGCA GAGAAGAAAG CAGCTTGAAG CACTGATGGC TGAACATCAG 
AGGAGGCAAG GTCTAGCTGA AACTACATCC CCTCTGGCTG TGTCATTGAG AAGTGATGGA 
TCTGAGAACC TGTGTACGCC TCAACAGAGT AGAACAGAAA AAACCATGGC AACATGGGGA 
GGTTCTACCC AGTGTGCACT AGATGAAGAA GGAGATGAAG ATGGTTACCT TTCTGAAGGA 
GTTGTTCGGA CAGATGAAGA GGAGGAAGAA GAAGAGCAAG ATGCAAGTTC GAATGATAAC 
TTTTCTATGT ATCCTCCTAA CAGTGCGAAT CATAACTCTT ACAACATAAA AGAAACTAAG 
AATAGGTGGA AGAATAGTCG CCCTTTTACA GCAGATGGAA ATTATCGTCC TTTAGCCAAG 
ACAAGACAGC AGAATATCAG CATGCAACGT CAGGAAAACC TTCGTTGGAT GTCCGAACTC 
TCTTACGTAG AGGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTGAA GAAACAACTT 
GATTTCAGTG TCAATATTTG TCAGACTTTG ATGCAAGACC AGCAGACGCT GTCTTGTCTG 
CTGCAAACTC TTCTCACAGG TCCTTACAGT GTTATGCCCA GCAATGTAGC TTCTCCTCAA 
GTACACCTTA TAATGCACCA GTTGAATCAG TGCTATACTC AGCTAACATG GCAACAGAAT 
AATGTGCAAA GGTTGAAACA AATGCTAAAT GAACTTATGC GCCAACAGAA TCAGCACCCA 
GAAAAACCTG GAAGCCAGGA AAGAGGCAGT AGTGCACCAC AGCCTTCTTC TCCCAGTTTA 
TTTTGTCCTT TCAGCTTTCC ATCACAGCCT GTAAATCTAT TTAACCTACC CGGGTTTACT 
AATTTTTCAT CATTTGCACC AGGTATGAAT TTTAGCCCTT TATTTCCTTC TAATTTTGGA 
GAATTTTCTC AGAATATTTC TACACCTACT GAACAGCAGC AACCATTAGC CCAGAATTCT 
TCAGGGAAAA CAGAATATAT GGCTTTTCCA AAACCTTTTG AAAGCAGTTC TTCTATTGGA 
GCAGAAAAAC AAAGGAATCA AAAACAGCCT GGAGAGGAGG TGGAAAACAG TAGGACAGCG 
TGGTTATATG ACCAAGAAGG TGAAGTAGAA AAACCATTTA TCAAGACTGG ATTTCCAGTG 
TCTGTAGAGA AAACTACAAA TAGTAATCGC AAAAATCAAT TAGATACCGG CCGAAGAAGA 
CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC TTTAGCAGTA TGCCTGATCC AGTAGATCCG 
ACAACAGTAA CCAAAACATT CAAGACGAGA AAAGCATCTG CACAGGCCAG CCTAGCATCT 
AAAGATAAAA CTCCTAAATC AAAGAGTAAG AAAAGGCATT CTGCTCAACT AAAAAGCAGA 
GTTAAAAATA CTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCTTGAGC AGCTGGAAAA AATAATAAAA TATAGTAGGT CTACAGAGAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC ACCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGGGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCATCCACAT TTTCTTATTG AACTCTTCCA TGAACTTCAG 
CTACTTAATA CAGACTACTT GAGACAAAGG GCTTTATATG CATTGCAGGA CATAGTAACC 
AGACATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATG TAAAGTCAGT GAATTCTGGT 
ACTTGGATAG CATCAAACTC AGAGCTTACC CCCAGTGAAA GCCTTGCTAC CACTGATGAT 
GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT 
GATAATGCTA GCGTCATGTC TGTATCTTCA AATTTTGAGC CTTTTGCAAC AGATGATCTA 
GGTAACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGGGAATA CGAGCGTATG 
AAGACTGAGG CTGAAAGTAG CACAAATATA AGATGCACCT GCAGAATTCT TGAGGATGAG 
GATGGTGCTG CCGCCACAAG TATGGTTACT AATTTAGAAG AAACTCCTAT TGAAAATCAT 
GGTTCACAAC AACCTGTAAG TGAAGTTTCT ACTGTTCCAT GTCCTAGAAT TGATACTCAG 
CAGCTGGACC GGCAAATTAA AGCCATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC 
ATGGATGAAG TATGCTCTTC TCAACTTCTA ACTTCAGTAA GGCGTATGGT TTTGACCCTC 
ACCCAACAAA ATGATGAGAG CAAAGAATTT GTGAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTT AATGAATTGG CTTTCTTTAA GCTCATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT 
GGAGTGATAC AGTCTTATGC CAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCATGGCTCA 
CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AAACTGAAAC AGTTAAGCCG 
ACTCAGACAT CTGAGATATA TGATGGTGAT GGTCCCAAAA ATGTGAGATC AGATGTTTCT 
GATCAAGAGG AAGATGAAGA AAGTGAAGAA TGTCCGGTGT CTATTAACTT GTCCAAAGCT 
GAAACTCAGG CTTTGACTAA TTATGGAAGT GGAGAAGATG AGAATGAGGA TGAAGAAATA 
GAAGAATTTG AAGAGGGACC TGTGGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAGCT 
ACAGAAGAAA CTGAACACGA TGATCAGGTA CTACAACATG ACTTTGAAAA ATCAGGAGAA 
AGCAAAAATG TCCCATCAGA ACAAGATCCC ACTACTAGTA AAGGCAAGAA ATATGACCAA 
GATAGTACTC CTGTGAAACC CTGTTACCTC AATATCTTGG AAAATGAGCA GCCTTTGAAT 
AGCGCTGTCC AAAAAGACTC TCTGACTACC ATCGATTCAT CTAAACAGCC TAACCCTTTG 
CCTTTACCTT TACCTGAAAT CGAAACCTTG GTGCCTACAG TCAAAGAAGT TAAATCTGCT 
CAAGAAACTC CTGAAAGCTC TCTGGCTGGA AGTCCTGATA CTGAATCTCC AGTGTTAGTG 
AATGACTATG AAGCAGAATC TGGTAACATA AGTCAAAAAT CTGATGAAGA AGACTTTGTG 
AAAGTTGAAG ATTTACCACT TAAACTGACA ATATACTCAG AGGCAGATCT AAGGAAGAAA 
ATGGTAGAAG AGCAAGAGAA AAACCATTTA TCTGGTGAAA TACTATGTGA AATGCAGACC 
GAAGAATTAG CTGGGAATTC TCAGACACTA AAAGAACCTG AAACAGTGGG AGCCCAAAGC 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630010.1
CDS	616..7101
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629953.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629953.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGTTG TAACAGAAAC TACAGGTAGC GTATCTGGAG TTAGTATCAC CTCTGAACTA 
AATGAAGAAT TGAATGATTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAACTGTTC CAGACAATAG AAGACAAGCA GAAAGTCTTT CATTAACTAG GGAGGTATCC 
CAGAGCAGGA ATCCATCAGT TTCAGAACAC TTACCTGATG AGAAAGTACA ACTGTTTAGC 
AAAATGAGAG TATTACAGGA AAAGAAACAA AAAATGGACA AACTACTTGG AGAACTTCAT 
ACACTTCGAG ATCAACATCT GAACAATTCA TCATTTGTGC CTTCTTCAGC TTCTCCACAA 
AGAAGTGTGG ATCAGAGAAG TACAACTTCA GCACCCTCTG CTCCTATAGG CTTAGCACCA 
GTTGTCAATG GAGAATCCAA CAGCTTCACA TCATCTGTTC CTTATCCTGT TGCTTCTCTA 
GTTTCTCAGA ATGAGAGTGA AAACGAAGGA CATCTAAATC CAACTGAAAA ACTCCAGAAG 
TTAAATGAAG TTCGAAAGAG ATTGAATGAA CTAAGAGAAC TAGTTCATTA TTACGAACAA 
ACATCAGATA TGATGACAGA TGCTGTAAAT GAAAACACAA AAGATGAAGA AACTGAAGAG 
TCAGAATATG ATTCTGAACA TGAAAACCCT GAACCTGTTA CTAACATTCG AAATCCTCAA 
GTAGCTGCCA CTTGGAATGA AGTAAATAGT AATTCTAATG CACAGTGTGT TTCTAATAAT 
AGAGAGGGGA GATCAGTTAA TTCTAATTGT GAAATTAACA ACAGATCTGC TGCCAACATA 
AGGACTCTGA ACATGCCTCC TTCTTTAGCA GATTGTCACT ATAATAGAGA AGGAGAACAG 
GGGATTCATG GTGCACAAGG TGAAGACGAT GAGGAAGAAG AAGAAGCAGA AGATGAAGGA 
GTCAGTGGGG CTTCATTAAC TAGTCATAGG AGCAGTCTGG TTGATGAGGC TGCAGAAGAC 
GCTGAATTTG AACAGAAGAT CAATAGACTT ATGGCTGCCA AACAGAAATT AAGACAGTTA 
CAAGATCTTG TTGCTATGGT ACAGGATGAT GATGCAGCAG ATCATGGAGT TATCTCTGCC 
AATACTTCAA ATTTGGATGA TTTTTACCCA GCAGAAGAAG ACAACAAACA AAGTGCAAAT 
AATACTAGAG GAAATGCCAA TAAAACACAG AAAGATGCTG GAATAAATGA GAAGGCAAGA 
GAGAAGTTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AGCTCAGACA ATTGCAGGAA 
GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG CATTACAGAA GGCATGCCCT 
GACTTACAGC TGTCAGCCAC TAGTGCGGGT AATTGTCCCA CAAAAAAATA TATCCCAGCT 
GTTACTTCAA CCCCAGTTGT TAATGGGAAT GAAACCAGTA CAAGCAAATC TGCTTTTGAG 
CCTGCAGATC CTTCAGGAGT GGATAATGAG CTGTGGTCAG AAATGCGAAG ACATGAGATG 
TTAAGGGAAG AGCTACGGCA GAGAAGAAAG CAGCTTGAAG CACTGATGGC TGAACATCAG 
AGGAGGCAAG GTCTAGCTGA AACTACATCC CCTCTGGCTG TGTCATTGAG AAGTGATGGA 
TCTGAGAACC TGTGTACGCC TCAACAGAGT AGAACAGAAA AAACCATGGC AACATGGGGA 
GGTTCTACCC AGTGTGCACT AGATGAAGAA GGAGATGAAG ATGGTTACCT TTCTGAAGGA 
GTTGTTCGGA CAGATGAAGA GGAGGAAGAA GAAGAGCAAG ATGCAAGTTC GAATGATAAC 
TTTTCTATGT ATCCTCCTAA CAGTGCGAAT CATAACTCTT ACAACATAAA AGAAACTAAG 
AATAGGTGGA AGAATAGTCG CCCTTTTACA GCAGATGGAA ATTATCGTCC TTTAGCCAAG 
ACAAGACAGC AGAATATCAG CATGCAACGT CAGGAAAACC TTCGTTGGAT GTCCGAACTC 
TCTTACGTAG AGGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTGAA GAAACAACTT 
GATTTCAGTG TCAATATTTG TCAGACTTTG ATGCAAGACC AGCAGACGCT GTCTTGTCTG 
CTGCAAACTC TTCTCACAGG TCCTTACAGT GTTATGCCCA GCAATGTAGC TTCTCCTCAA 
GTACACCTTA TAATGCACCA GTTGAATCAG TGCTATACTC AGCTAACATG GCAACAGAAT 
AATGTGCAAA GGTTGAAACA AATGCTAAAT GAACTTATGC GCCAACAGAA TCAGCACCCA 
GAAAAACCTG GAAGCCAGGA AAGAGGCAGT AGTGCACCAC AGCCTTCTTC TCCCAGTTTA 
TTTTGTCCTT TCAGCTTTCC ATCACAGCCT GTAAATCTAT TTAACCTACC CGGGTTTACT 
AATTTTTCAT CATTTGCACC AGGTATGAAT TTTAGCCCTT TATTTCCTTC TAATTTTGGA 
GAATTTTCTC AGAATATTTC TACACCTACT GAACAGCAGC AACCATTAGC CCAGAATTCT 
TCAGGGAAAA CAGAATATAT GGCTTTTCCA AAACCTTTTG AAAGCAGTTC TTCTATTGGA 
GCAGAAAAAC AAAGGAATCA AAAACAGCCT GGAGAGGAGG TGGAAAACAG TAGGACAGCG 
TGGTTATATG ACCAAGAAGG TGAAGTAGAA AAACCATTTA TCAAGACTGG ATTTCCAGTG 
TCTGTAGAGA AAACTACAAA TAGTAATCGC AAAAATCAAT TAGATACCGG CCGAAGAAGA 
CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC TTTAGCAGTA TGCCTGATCC AGTAGATCCG 
ACAACAGTAA CCAAAACATT CAAGACGAGA AAAGCATCTG CACAGGCCAG CCTAGCATCT 
AAAGATAAAA CTCCTAAATC AAAGAGTAAG AAAAGGCATT CTGCTCAACT AAAAAGCAGA 
GTTAAAAATA CTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCTTGAGC AGCTGGAAAA AATAATAAAA TATAGTAGGT CTACAGAGAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC ACCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGGGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCATCCACAT TTTCTTATTG AACTCTTCCA TGAACTTCAG 
CTACTTAATA CAGACTACTT GAGACAAAGG GCTTTATATG CATTGCAGGA CATAGTAACC 
AGACATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATG TAAAGTCAGT GAATTCTGGT 
ACTTGGATAG CATCAAACTC AGAGCTTACC CCCAGTGAAA GCCTTGCTAC CACTGATGAT 
GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT 
GATAATGCTA GCGTCATGTC TGTATCTTCA AATTTTGAGC CTTTTGCAAC AGATGATCTA 
GGTAACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGGGAATA CGAGCGTATG 
AAGACTGAGG CTGAAAGTAG CACAAATATA AGATGCACCT GCAGAATTCT TGAGGATGAG 
GATGGTGCTG CCGCCACAAG TATGGTTACT AATTTAGAAG AAACTCCTAT TGAAAATCAT 
GGTTCACAAC AACCTGTAAG TGAAGTTTCT ACTGTTCCAT GTCCTAGAAT TGATACTCAG 
CAGCTGGACC GGCAAATTAA AGCCATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC 
ATGGATGAAG TATGCTCTTC TCAACTTCTA ACTTCAGTAA GGCGTATGGT TTTGACCCTC 
ACCCAACAAA ATGATGAGAG CAAAGAATTT GTGAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTT AATGAATTGG CTTTCTTTAA GCTCATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT 
GGAGTGATAC AGTCTTATGC CAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCATGGCTCA 
CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AAACTGAAAC AGTTAAGCCG 
ACTCAGACAT CTGAGATATA TGATGGTGAT GGTCCCAAAA ATGTGAGATC AGATGTTTCT 
GATCAAGAGG AAGATGAAGA AAGTGAAGAA TGTCCGGTGT CTATTAACTT GTCCAAAGCT 
GAAACTCAGG CTTTGACTAA TTATGGAAGT GGAGAAGATG AGAATGAGGA TGAAGAAATA 
GAAGAATTTG AAGAGGGACC TGTGGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAGCT 
ACAGAAGAAA CTGAACACGA TGATCAGGTA CTACAACATG ACTTTGAAAA ATCAGGAGAA 
AGCAAAAATG TCCCATCAGA ACAAGATCCC ACTACTAGTA AAGGCAAGAA ATATGACCAA 
GATAGTACTC CTGTGAAACC CTGTTACCTC AATATCTTGG AAAATGAGCA GCCTTTGAAT 
AGCGCTGTCC AAAAAGACTC TCTGACTACC ATCGATTCAT CTAAACAGCC TAACCCTTTG 
CCTTTACCTT TACCTGAAAT CGAAACCTTG GTGCCTACAG TCAAAGAAGT TAAATCTGCT 
CAAGAAACTC CTGAAAGCTC TCTGGCTGGA AGTCCTGATA CTGAATCTCC AGTGTTAGTG 
AATGACTATG AAGCAGAATC TGGTAACATA AGTCAAAAAT CTGATGAAGA AGACTTTGTG 
AAAGTTGAAG ATTTACCACT TAAACTGACA ATATACTCAG AGGCAGATCT AAGGAAGAAA 
ATGGTAGAAG AGCAAGAGAA AAACCATTTA TCTGGTGAAA TACTATGTGA AATGCAGACC 
GAAGAATTAG CTGGGAATTC TCAGACACTA AAAGAACCTG AAACAGTGGG AGCCCAAAGC 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404150.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259858.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X11, mRNA.

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CloneID	OCe18439
Clone ID Related Accession (Same CDS sequence)	XM_005629953.1 , XM_022404150.1
Accession Version	XM_022404150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGTTG TAACAGAAAC TACAGGTAGC GTATCTGGAG TTAGTATCAC CTCTGAACTA 
AATGAAGAAT TGAATGATTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAACTGTTC CAGACAATAG AAGACAAGCA GAAAGTCTTT CATTAACTAG GGAGGTATCC 
CAGAGCAGGA ATCCATCAGT TTCAGAACAC TTACCTGATG AGAAAGTACA ACTGTTTAGC 
AAAATGAGAG TATTACAGGA AAAGAAACAA AAAATGGACA AACTACTTGG AGAACTTCAT 
ACACTTCGAG ATCAACATCT GAACAATTCA TCATTTGTGC CTTCTTCAGC TTCTCCACAA 
AGAAGTGTGG ATCAGAGAAG TACAACTTCA GCACCCTCTG CTCCTATAGG CTTAGCACCA 
GTTGTCAATG GAGAATCCAA CAGCTTCACA TCATCTGTTC CTTATCCTGT TGCTTCTCTA 
GTTTCTCAGA ATGAGAGTGA AAACGAAGGA CATCTAAATC CAACTGAAAA ACTCCAGAAG 
TTAAATGAAG TTCGAAAGAG ATTGAATGAA CTAAGAGAAC TAGTTCATTA TTACGAACAA 
ACATCAGATA TGATGACAGA TGCTGTAAAT GAAAACACAA AAGATGAAGA AACTGAAGAG 
TCAGAATATG ATTCTGAACA TGAAAACCCT GAACCTGTTA CTAACATTCG AAATCCTCAA 
GTAGCTGCCA CTTGGAATGA AGTAAATAGT AATTCTAATG CACAGTGTGT TTCTAATAAT 
AGAGAGGGGA GATCAGTTAA TTCTAATTGT GAAATTAACA ACAGATCTGC TGCCAACATA 
AGGACTCTGA ACATGCCTCC TTCTTTAGCA GATTGTCACT ATAATAGAGA AGGAGAACAG 
GGGATTCATG GTGCACAAGG TGAAGACGAT GAGGAAGAAG AAGAAGCAGA AGATGAAGGA 
GTCAGTGGGG CTTCATTAAC TAGTCATAGG AGCAGTCTGG TTGATGAGGC TGCAGAAGAC 
GCTGAATTTG AACAGAAGAT CAATAGACTT ATGGCTGCCA AACAGAAATT AAGACAGTTA 
CAAGATCTTG TTGCTATGGT ACAGGATGAT GATGCAGCAG ATCATGGAGT TATCTCTGCC 
AATACTTCAA ATTTGGATGA TTTTTACCCA GCAGAAGAAG ACAACAAACA AAGTGCAAAT 
AATACTAGAG GAAATGCCAA TAAAACACAG AAAGATGCTG GAATAAATGA GAAGGCAAGA 
GAGAAGTTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AGCTCAGACA ATTGCAGGAA 
GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG CATTACAGAA GGCATGCCCT 
GACTTACAGC TGTCAGCCAC TAGTGCGGGT AATTGTCCCA CAAAAAAATA TATCCCAGCT 
GTTACTTCAA CCCCAGTTGT TAATGGGAAT GAAACCAGTA CAAGCAAATC TGCTTTTGAG 
CCTGCAGATC CTTCAGGAGT GGATAATGAG CTGTGGTCAG AAATGCGAAG ACATGAGATG 
TTAAGGGAAG AGCTACGGCA GAGAAGAAAG CAGCTTGAAG CACTGATGGC TGAACATCAG 
AGGAGGCAAG GTCTAGCTGA AACTACATCC CCTCTGGCTG TGTCATTGAG AAGTGATGGA 
TCTGAGAACC TGTGTACGCC TCAACAGAGT AGAACAGAAA AAACCATGGC AACATGGGGA 
GGTTCTACCC AGTGTGCACT AGATGAAGAA GGAGATGAAG ATGGTTACCT TTCTGAAGGA 
GTTGTTCGGA CAGATGAAGA GGAGGAAGAA GAAGAGCAAG ATGCAAGTTC GAATGATAAC 
TTTTCTATGT ATCCTCCTAA CAGTGCGAAT CATAACTCTT ACAACATAAA AGAAACTAAG 
AATAGGTGGA AGAATAGTCG CCCTTTTACA GCAGATGGAA ATTATCGTCC TTTAGCCAAG 
ACAAGACAGC AGAATATCAG CATGCAACGT CAGGAAAACC TTCGTTGGAT GTCCGAACTC 
TCTTACGTAG AGGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTGAA GAAACAACTT 
GATTTCAGTG TCAATATTTG TCAGACTTTG ATGCAAGACC AGCAGACGCT GTCTTGTCTG 
CTGCAAACTC TTCTCACAGG TCCTTACAGT GTTATGCCCA GCAATGTAGC TTCTCCTCAA 
GTACACCTTA TAATGCACCA GTTGAATCAG TGCTATACTC AGCTAACATG GCAACAGAAT 
AATGTGCAAA GGTTGAAACA AATGCTAAAT GAACTTATGC GCCAACAGAA TCAGCACCCA 
GAAAAACCTG GAAGCCAGGA AAGAGGCAGT AGTGCACCAC AGCCTTCTTC TCCCAGTTTA 
TTTTGTCCTT TCAGCTTTCC ATCACAGCCT GTAAATCTAT TTAACCTACC CGGGTTTACT 
AATTTTTCAT CATTTGCACC AGGTATGAAT TTTAGCCCTT TATTTCCTTC TAATTTTGGA 
GAATTTTCTC AGAATATTTC TACACCTACT GAACAGCAGC AACCATTAGC CCAGAATTCT 
TCAGGGAAAA CAGAATATAT GGCTTTTCCA AAACCTTTTG AAAGCAGTTC TTCTATTGGA 
GCAGAAAAAC AAAGGAATCA AAAACAGCCT GGAGAGGAGG TGGAAAACAG TAGGACAGCG 
TGGTTATATG ACCAAGAAGG TGAAGTAGAA AAACCATTTA TCAAGACTGG ATTTCCAGTG 
TCTGTAGAGA AAACTACAAA TAGTAATCGC AAAAATCAAT TAGATACCGG CCGAAGAAGA 
CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC TTTAGCAGTA TGCCTGATCC AGTAGATCCG 
ACAACAGTAA CCAAAACATT CAAGACGAGA AAAGCATCTG CACAGGCCAG CCTAGCATCT 
AAAGATAAAA CTCCTAAATC AAAGAGTAAG AAAAGGCATT CTGCTCAACT AAAAAGCAGA 
GTTAAAAATA CTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCTTGAGC AGCTGGAAAA AATAATAAAA TATAGTAGGT CTACAGAGAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC ACCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGGGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCATCCACAT TTTCTTATTG AACTCTTCCA TGAACTTCAG 
CTACTTAATA CAGACTACTT GAGACAAAGG GCTTTATATG CATTGCAGGA CATAGTAACC 
AGACATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATG TAAAGTCAGT GAATTCTGGT 
ACTTGGATAG CATCAAACTC AGAGCTTACC CCCAGTGAAA GCCTTGCTAC CACTGATGAT 
GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT 
GATAATGCTA GCGTCATGTC TGTATCTTCA AATTTTGAGC CTTTTGCAAC AGATGATCTA 
GGTAACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGGGAATA CGAGCGTATG 
AAGACTGAGG CTGAAAGTAG CACAAATATA AGATGCACCT GCAGAATTCT TGAGGATGAG 
GATGGTGCTG CCGCCACAAG TATGGTTACT AATTTAGAAG AAACTCCTAT TGAAAATCAT 
GGTTCACAAC AACCTGTAAG TGAAGTTTCT ACTGTTCCAT GTCCTAGAAT TGATACTCAG 
CAGCTGGACC GGCAAATTAA AGCCATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC 
ATGGATGAAG TATGCTCTTC TCAACTTCTA ACTTCAGTAA GGCGTATGGT TTTGACCCTC 
ACCCAACAAA ATGATGAGAG CAAAGAATTT GTGAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTT AATGAATTGG CTTTCTTTAA GCTCATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT 
GGAGTGATAC AGTCTTATGC CAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCATGGCTCA 
CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AAACTGAAAC AGTTAAGCCG 
ACTCAGACAT CTGAGATATA TGATGGTGAT GGTCCCAAAA ATGTGAGATC AGATGTTTCT 
GATCAAGAGG AAGATGAAGA AAGTGAAGAA TGTCCGGTGT CTATTAACTT GTCCAAAGCT 
GAAACTCAGG CTTTGACTAA TTATGGAAGT GGAGAAGATG AGAATGAGGA TGAAGAAATA 
GAAGAATTTG AAGAGGGACC TGTGGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAGCT 
ACAGAAGAAA CTGAACACGA TGATCAGGTA CTACAACATG ACTTTGAAAA ATCAGGAGAA 
AGCAAAAATG TCCCATCAGA ACAAGATCCC ACTACTAGTA AAGGCAAGAA ATATGACCAA 
GATAGTACTC CTGTGAAACC CTGTTACCTC AATATCTTGG AAAATGAGCA GCCTTTGAAT 
AGCGCTGTCC AAAAAGACTC TCTGACTACC ATCGATTCAT CTAAACAGCC TAACCCTTTG 
CCTTTACCTT TACCTGAAAT CGAAACCTTG GTGCCTACAG TCAAAGAAGT TAAATCTGCT 
CAAGAAACTC CTGAAAGCTC TCTGGCTGGA AGTCCTGATA CTGAATCTCC AGTGTTAGTG 
AATGACTATG AAGCAGAATC TGGTAACATA AGTCAAAAAT CTGATGAAGA AGACTTTGTG 
AAAGTTGAAG ATTTACCACT TAAACTGACA ATATACTCAG AGGCAGATCT AAGGAAGAAA 
ATGGTAGAAG AGCAAGAGAA AAACCATTTA TCTGGTGAAA TACTATGTGA AATGCAGACC 
GAAGAATTAG CTGGGAATTC TCAGACACTA AAAGAACCTG AAACAGTGGG AGCCCAAAGC 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259858.1
CDS	426..6911
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404150.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404150.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGTTG TAACAGAAAC TACAGGTAGC GTATCTGGAG TTAGTATCAC CTCTGAACTA 
AATGAAGAAT TGAATGATTT AATTCAGCGT TTTCATAATC AGCTTCGTGA TTCTCAGCCT 
CCAACTGTTC CAGACAATAG AAGACAAGCA GAAAGTCTTT CATTAACTAG GGAGGTATCC 
CAGAGCAGGA ATCCATCAGT TTCAGAACAC TTACCTGATG AGAAAGTACA ACTGTTTAGC 
AAAATGAGAG TATTACAGGA AAAGAAACAA AAAATGGACA AACTACTTGG AGAACTTCAT 
ACACTTCGAG ATCAACATCT GAACAATTCA TCATTTGTGC CTTCTTCAGC TTCTCCACAA 
AGAAGTGTGG ATCAGAGAAG TACAACTTCA GCACCCTCTG CTCCTATAGG CTTAGCACCA 
GTTGTCAATG GAGAATCCAA CAGCTTCACA TCATCTGTTC CTTATCCTGT TGCTTCTCTA 
GTTTCTCAGA ATGAGAGTGA AAACGAAGGA CATCTAAATC CAACTGAAAA ACTCCAGAAG 
TTAAATGAAG TTCGAAAGAG ATTGAATGAA CTAAGAGAAC TAGTTCATTA TTACGAACAA 
ACATCAGATA TGATGACAGA TGCTGTAAAT GAAAACACAA AAGATGAAGA AACTGAAGAG 
TCAGAATATG ATTCTGAACA TGAAAACCCT GAACCTGTTA CTAACATTCG AAATCCTCAA 
GTAGCTGCCA CTTGGAATGA AGTAAATAGT AATTCTAATG CACAGTGTGT TTCTAATAAT 
AGAGAGGGGA GATCAGTTAA TTCTAATTGT GAAATTAACA ACAGATCTGC TGCCAACATA 
AGGACTCTGA ACATGCCTCC TTCTTTAGCA GATTGTCACT ATAATAGAGA AGGAGAACAG 
GGGATTCATG GTGCACAAGG TGAAGACGAT GAGGAAGAAG AAGAAGCAGA AGATGAAGGA 
GTCAGTGGGG CTTCATTAAC TAGTCATAGG AGCAGTCTGG TTGATGAGGC TGCAGAAGAC 
GCTGAATTTG AACAGAAGAT CAATAGACTT ATGGCTGCCA AACAGAAATT AAGACAGTTA 
CAAGATCTTG TTGCTATGGT ACAGGATGAT GATGCAGCAG ATCATGGAGT TATCTCTGCC 
AATACTTCAA ATTTGGATGA TTTTTACCCA GCAGAAGAAG ACAACAAACA AAGTGCAAAT 
AATACTAGAG GAAATGCCAA TAAAACACAG AAAGATGCTG GAATAAATGA GAAGGCAAGA 
GAGAAGTTTT ATGAGGCTAA ACTACAGCAG CAACAGAGAG AGCTCAGACA ATTGCAGGAA 
GAAAGAAAGA AACTGATTGA GATTCAAGAG AAAATTCAAG CATTACAGAA GGCATGCCCT 
GACTTACAGC TGTCAGCCAC TAGTGCGGGT AATTGTCCCA CAAAAAAATA TATCCCAGCT 
GTTACTTCAA CCCCAGTTGT TAATGGGAAT GAAACCAGTA CAAGCAAATC TGCTTTTGAG 
CCTGCAGATC CTTCAGGAGT GGATAATGAG CTGTGGTCAG AAATGCGAAG ACATGAGATG 
TTAAGGGAAG AGCTACGGCA GAGAAGAAAG CAGCTTGAAG CACTGATGGC TGAACATCAG 
AGGAGGCAAG GTCTAGCTGA AACTACATCC CCTCTGGCTG TGTCATTGAG AAGTGATGGA 
TCTGAGAACC TGTGTACGCC TCAACAGAGT AGAACAGAAA AAACCATGGC AACATGGGGA 
GGTTCTACCC AGTGTGCACT AGATGAAGAA GGAGATGAAG ATGGTTACCT TTCTGAAGGA 
GTTGTTCGGA CAGATGAAGA GGAGGAAGAA GAAGAGCAAG ATGCAAGTTC GAATGATAAC 
TTTTCTATGT ATCCTCCTAA CAGTGCGAAT CATAACTCTT ACAACATAAA AGAAACTAAG 
AATAGGTGGA AGAATAGTCG CCCTTTTACA GCAGATGGAA ATTATCGTCC TTTAGCCAAG 
ACAAGACAGC AGAATATCAG CATGCAACGT CAGGAAAACC TTCGTTGGAT GTCCGAACTC 
TCTTACGTAG AGGAGAAAGA ACAATGGCAA GAACAAATTA ATCAGCTGAA GAAACAACTT 
GATTTCAGTG TCAATATTTG TCAGACTTTG ATGCAAGACC AGCAGACGCT GTCTTGTCTG 
CTGCAAACTC TTCTCACAGG TCCTTACAGT GTTATGCCCA GCAATGTAGC TTCTCCTCAA 
GTACACCTTA TAATGCACCA GTTGAATCAG TGCTATACTC AGCTAACATG GCAACAGAAT 
AATGTGCAAA GGTTGAAACA AATGCTAAAT GAACTTATGC GCCAACAGAA TCAGCACCCA 
GAAAAACCTG GAAGCCAGGA AAGAGGCAGT AGTGCACCAC AGCCTTCTTC TCCCAGTTTA 
TTTTGTCCTT TCAGCTTTCC ATCACAGCCT GTAAATCTAT TTAACCTACC CGGGTTTACT 
AATTTTTCAT CATTTGCACC AGGTATGAAT TTTAGCCCTT TATTTCCTTC TAATTTTGGA 
GAATTTTCTC AGAATATTTC TACACCTACT GAACAGCAGC AACCATTAGC CCAGAATTCT 
TCAGGGAAAA CAGAATATAT GGCTTTTCCA AAACCTTTTG AAAGCAGTTC TTCTATTGGA 
GCAGAAAAAC AAAGGAATCA AAAACAGCCT GGAGAGGAGG TGGAAAACAG TAGGACAGCG 
TGGTTATATG ACCAAGAAGG TGAAGTAGAA AAACCATTTA TCAAGACTGG ATTTCCAGTG 
TCTGTAGAGA AAACTACAAA TAGTAATCGC AAAAATCAAT TAGATACCGG CCGAAGAAGA 
CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC TTTAGCAGTA TGCCTGATCC AGTAGATCCG 
ACAACAGTAA CCAAAACATT CAAGACGAGA AAAGCATCTG CACAGGCCAG CCTAGCATCT 
AAAGATAAAA CTCCTAAATC AAAGAGTAAG AAAAGGCATT CTGCTCAACT AAAAAGCAGA 
GTTAAAAATA CTGGGTATGA AAGTGCCAGT GTGTCTAGCA CATGTGAACC TTGCAAAAGT 
AGGAACAGAC ATTCAGCCCA GACTGAAGAG CCTGTTCAAG CAAAAGTATT CAGCAGAAAG 
AATCTTGAGC AGCTGGAAAA AATAATAAAA TATAGTAGGT CTACAGAGAT ATCTTCAGCA 
CATGCTAGGA GAATACTACA GCAGTCTAAC AGAAATGCAT GCAATGAAGC ACCAGAAACT 
GGGAGTGATT TTTCCATGTT TGAAGCTTTG CGGGATACTA TTTATTCTGA AGTAGCTACA 
TTAATTTCTC AAAATGAATC TCATCCACAT TTTCTTATTG AACTCTTCCA TGAACTTCAG 
CTACTTAATA CAGACTACTT GAGACAAAGG GCTTTATATG CATTGCAGGA CATAGTAACC 
AGACATATTT CTGAGAACCA TGAAAAAGAA GGAGAAAATG TAAAGTCAGT GAATTCTGGT 
ACTTGGATAG CATCAAACTC AGAGCTTACC CCCAGTGAAA GCCTTGCTAC CACTGATGAT 
GAAACTTTTG AGAAGAACTT TGAGAGAGAA ACACATAAAA TAAGTGAGCA AAATGATGCT 
GATAATGCTA GCGTCATGTC TGTATCTTCA AATTTTGAGC CTTTTGCAAC AGATGATCTA 
GGTAACACTG TGATTCACTT AGATCAGGCA TTAGCCAGAA TGAGGGAATA CGAGCGTATG 
AAGACTGAGG CTGAAAGTAG CACAAATATA AGATGCACCT GCAGAATTCT TGAGGATGAG 
GATGGTGCTG CCGCCACAAG TATGGTTACT AATTTAGAAG AAACTCCTAT TGAAAATCAT 
GGTTCACAAC AACCTGTAAG TGAAGTTTCT ACTGTTCCAT GTCCTAGAAT TGATACTCAG 
CAGCTGGACC GGCAAATTAA AGCCATTATG AAAGAAGTCA TTCCTTTTTT GAAGGAGCAC 
ATGGATGAAG TATGCTCTTC TCAACTTCTA ACTTCAGTAA GGCGTATGGT TTTGACCCTC 
ACCCAACAAA ATGATGAGAG CAAAGAATTT GTGAAGTTCT TTCATAAACA ACTTGGAAGT 
ATATTACAGG ATTCACTGGC AAAATTTGCT GGCAGAAAAC TGAAAGACTG TGGAGAAGAT 
CTTCTTGTAG AGATATCTGA AGTGTTGTTT AATGAATTGG CTTTCTTTAA GCTCATGCAA 
GATTTGGATA ATAATAGTAT AACTGTTAAA CAGAAGTGCA AAAGGAAAAT AGAAGCAGCT 
GGAGTGATAC AGTCTTATGC CAAAGAGGCC AAAAGGATTC TTGAAGGAGA TCATGGCTCA 
CCTGCTGGAG AAATTGATGA TGAAGACAAA GACAAGGATG AAACTGAAAC AGTTAAGCCG 
ACTCAGACAT CTGAGATATA TGATGGTGAT GGTCCCAAAA ATGTGAGATC AGATGTTTCT 
GATCAAGAGG AAGATGAAGA AAGTGAAGAA TGTCCGGTGT CTATTAACTT GTCCAAAGCT 
GAAACTCAGG CTTTGACTAA TTATGGAAGT GGAGAAGATG AGAATGAGGA TGAAGAAATA 
GAAGAATTTG AAGAGGGACC TGTGGATGTC CAGACGTCAC TTCAGGCTAA CACTGAAGCT 
ACAGAAGAAA CTGAACACGA TGATCAGGTA CTACAACATG ACTTTGAAAA ATCAGGAGAA 
AGCAAAAATG TCCCATCAGA ACAAGATCCC ACTACTAGTA AAGGCAAGAA ATATGACCAA 
GATAGTACTC CTGTGAAACC CTGTTACCTC AATATCTTGG AAAATGAGCA GCCTTTGAAT 
AGCGCTGTCC AAAAAGACTC TCTGACTACC ATCGATTCAT CTAAACAGCC TAACCCTTTG 
CCTTTACCTT TACCTGAAAT CGAAACCTTG GTGCCTACAG TCAAAGAAGT TAAATCTGCT 
CAAGAAACTC CTGAAAGCTC TCTGGCTGGA AGTCCTGATA CTGAATCTCC AGTGTTAGTG 
AATGACTATG AAGCAGAATC TGGTAACATA AGTCAAAAAT CTGATGAAGA AGACTTTGTG 
AAAGTTGAAG ATTTACCACT TAAACTGACA ATATACTCAG AGGCAGATCT AAGGAAGAAA 
ATGGTAGAAG AGCAAGAGAA AAACCATTTA TCTGGTGAAA TACTATGTGA AATGCAGACC 
GAAGAATTAG CTGGGAATTC TCAGACACTA AAAGAACCTG AAACAGTGGG AGCCCAAAGC 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629954.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630011.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X13, mRNA.

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CloneID	OCe18440
Clone ID Related Accession (Same CDS sequence)	XM_005629954.2 , XM_022404151.1
Accession Version	XM_005629954.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAGCATGAC 
TTATTAAAAA GGATGTTACA ACAGCAGGAG CAACTGAGAG CTCTGCAGGG AAGACAGGCT 
GCTCTTCTTG CTCTGCAGCA CAAAGCAGAG CAAGCTATTG CTGTGATGGA TGATTCTGAA 
AAGGTTGCAG GGACAACACC TGGCCATCAT ACTGTACCGG GCAGCCAGCC AGCTCGCTCT 
CCTTTTCATC AGAGAGTACC CTTAAGAGTT GTAACAGAAA CTACAGGTAG CGTATCTGGA 
GTTAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCG TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAACTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGGTATC CCAGAGCAGG AATCCATCAG TTTCAGAACA CTTACCTGAT 
GAGAAAGTAC AACTGTTTAG CAAAATGAGA GTATTACAGG AAAAGAAACA AAAAATGGAC 
AAACTACTTG GAGAACTTCA TACACTTCGA GATCAACATC TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CTTCTCCACA AAGAAGTGTG GATCAGAGAA GTACAACTTC AGCACCCTCT 
GCTCCTATAG GCTTAGCACC AGTTGTCAAT GGAGAATCCA ACAGCTTCAC ATCATCTGTT 
CCTTATCCTG TTGCTTCTCT AGTTTCTCAG AATGAGAGTG AAAACGAAGG ACATCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GATTGAATGA ACTAAGAGAA 
CTAGTTCATT ATTACGAACA AACATCAGAT ATGATGACAG ATGCTGTAAA TGAAAACACA 
AAAGATGAAG AAACTGAAGA GTCAGAATAT GATTCTGAAC ATGAAAACCC TGAACCTGTT 
ACTAACATTC GAAATCCTCA AGTAGCTGCC ACTTGGAATG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCTAATAA TAGAGAGGGG AGATCAGTTA ATTCTAATTG TGAAATTAAC 
AACAGATCTG CTGCCAACAT AAGGACTCTG AACATGCCTC CTTCTTTAGC AGATTGTCAC 
TATAATAGAG AAGGAGAACA GGGGATTCAT GGTGCACAAG GTGAAGACGA TGAGGAAGAA 
GAAGAAGCAG AAGATGAAGG AGTCAGTGGG GCTTCATTAA CTAGTCATAG GAGCAGTCTG 
GTTGATGAGG CTGCAGAAGA CGCTGAATTT GAACAGAAGA TCAATAGACT TATGGCTGCC 
AAACAGAAAT TAAGACAGTT ACAAGATCTT GTTGCTATGG TACAGGATGA TGATGCAGCA 
GATCATGGAG TTATCTCTGC CAATACTTCA AATTTGGATG ATTTTTACCC AGCAGAAGAA 
GACAACAAAC AAAGTGCAAA TAATACTAGA GGAAATGCCA ATAAAACACA GAAAGATGCT 
GGAATAAATG AGAAGGCAAG AGAGAAGTTT TATGAGGCTA AACTACAGCA GCAACAGAGA 
GAGCTCAGAC AATTGCAGGA AGAAAGAAAG AAACTGATTG AGATTCAAGA GAAAATTCAA 
GCATTACAGA AGGCATGCCC TGACTTACAG CTGTCAGCCA CTAGTGCGGG TAATTGTCCC 
ACAAAAAAAT ATATCCCAGC TGTTACTTCA ACCCCAGTTG TTAATGGGAA TGAAACCAGT 
ACAAGCAAAT CTGCTTTTGA GCCTGCAGAT CCTTCAGGAG TGGATAATGA GCTGTGGTCA 
GAAATGCGAA GACATGAGAT GTTAAGGGAA GAGCTACGGC AGAGAAGAAA GCAGCTTGAA 
GCACTGATGG CTGAACATCA GAGGAGGCAA GGTCTAGCTG AAACTACATC CCCTCTGGCT 
GTGTCATTGA GAAGTGATGG ATCTGAGAAC CTGTGTACGC CTCAACAGAG TAGAACAGAA 
AAAACCATGG CAACATGGGG AGGTTCTACC CAGTGTGCAC TAGATGAAGA AGGAGATGAA 
GATGGTTACC TTTCTGAAGG AGTTGTTCGG ACAGATGAAG AGGAGGAAGA AGAAGAGCAA 
GATGCAAGTT CGAATGATAA CTTTTCTATG TATCCTCCTA ACAGTGCGAA TCATAACTCT 
TACAACATAA AAGAAACTAA GAATAGGTGG AAGAATAGTC GCCCTTTTAC AGCAGATGGA 
AATTATCGTC CTTTAGCCAA GACAAGACAG CAGAATATCA GCATGCAACG TCAGGAAAAC 
CTTCGTTGGA TGTCCGAACT CTCTTACGTA GAGGAGAAAG AACAATGGCA AGAACAAATT 
AATCAGCTGA AGAAACAACT TGATTTCAGT GTCAATATTT GTCAGACTTT GATGCAAGAC 
CAGCAGACGC TGTCTTGTCT GCTGCAAACT CTTCTCACAG GTCCTTACAG TGTTATGCCC 
AGCAATGTAG CTTCTCCTCA AGTACACCTT ATAATGCACC AGTTGAATCA GTGCTATACT 
CAGCTAACAT GGCAACAGAA TAATGTGCAA AGGTTGAAAC AAATGCTAAA TGAACTTATG 
CGCCAACAGA ATCAGCACCC AGAAAAACCT GGAAGCCAGG AAAGAGGCAG TAGTGCACCA 
CAGCCTTCTT CTCCCAGTTT ATTTTGTCCT TTCAGCTTTC CATCACAGCC TGTAAATCTA 
TTTAACCTAC CCGGGTTTAC TAATTTTTCA TCATTTGCAC CAGGTATGAA TTTTAGCCCT 
TTATTTCCTT CTAATTTTGG AGAATTTTCT CAGAATATTT CTACACCTAC TGAACAGCAG 
CAACCATTAG CCCAGAATTC TTCAGGGAAA ACAGAATATA TGGCTTTTCC AAAACCTTTT 
GAAAGCAGTT CTTCTATTGG AGCAGAAAAA CAAAGGAATC AAAAACAGCC TGGAGAGGAG 
GTGGAAAACA GTAGGACAGC GTGGTTATAT GACCAAGAAG GTGAAGTAGA AAAACCATTT 
ATCAAGACTG GATTTCCAGT GTCTGTAGAG AAAACTACAA ATAGTAATCG CAAAAATCAA 
TTAGATACCG GCCGAAGAAG ACGCCAGTTT GATGAAGAGT CATTAGAAAG CTTTAGCAGT 
ATGCCTGATC CAGTAGATCC GACAACAGTA ACCAAAACAT TCAAGACGAG AAAAGCATCT 
GCACAGGCCA GCCTAGCATC TAAAGATAAA ACTCCTAAAT CAAAGAGTAA GAAAAGGCAT 
TCTGCTCAAC TAAAAAGCAG AGTTAAAAAT ACTGGGTATG AAAGTGCCAG TGTGTCTAGC 
ACATGTGAAC CTTGCAAAAG TAGGAACAGA CATTCAGCCC AGACTGAAGA GCCTGTTCAA 
GCAAAAGTAT TCAGCAGAAA GAATCTTGAG CAGCTGGAAA AAATAATAAA ATATAGTAGG 
TCTACAGAGA TATCTTCAGC ACATGCTAGG AGAATACTAC AGCAGTCTAA CAGAAATGCA 
TGCAATGAAG CACCAGAAAC TGGGAGTGAT TTTTCCATGT TTGAAGCTTT GCGGGATACT 
ATTTATTCTG AAGTAGCTAC ATTAATTTCT CAAAATGAAT CTCATCCACA TTTTCTTATT 
GAACTCTTCC ATGAACTTCA GCTACTTAAT ACAGACTACT TGAGACAAAG GGCTTTATAT 
GCATTGCAGG ACATAGTAAC CAGACATATT TCTGAGAACC ATGAAAAAGA AGGAGAAAAT 
GTAAAGTCAG TGAATTCTGG TACTTGGATA GCATCAAACT CAGAGCTTAC CCCCAGTGAA 
AGCCTTGCTA CCACTGATGA TGAAACTTTT GAGAAGAACT TTGAGAGAGA AACACATAAA 
ATAAGTGAGC AAAATGATGC TGATAATGCT AGCGTCATGT CTGTATCTTC AAATTTTGAG 
CCTTTTGCAA CAGATGATCT AGGTAACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGGGAAT ACGAGCGTAT GAAGACTGAG GCTGAAAGTA GCACAAATAT AAGATGCACC 
TGCAGAATTC TTGAGGATGA GGATGGTGCT GCCGCCACAA GTATGGTTAC TAATTTAGAA 
GAAACTCCTA TTGAAAATCA TGGTTCACAA CAACCTGTAA GTGAAGTTTC TACTGTTCCA 
TGTCCTAGAA TTGATACTCA GCAGCTGGAC CGGCAAATTA AAGCCATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGCTCTT CTCAACTTCT AACTTCAGTA 
AGGCGTATGG TTTTGACCCT CACCCAACAA AATGATGAGA GCAAAGAATT TGTGAAGTTC 
TTTCATAAAC AACTTGGAAG TATATTACAG GATTCACTGG CAAAATTTGC TGGCAGAAAA 
CTGAAAGACT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTGTT TAATGAATTG 
GCTTTCTTTA AGCTCATGCA AGATTTGGAT AATAATAGTA TAACTGTTAA ACAGAAGTGC 
AAAAGGAAAA TAGAAGCAGC TGGAGTGATA CAGTCTTATG CCAAAGAGGC CAAAAGGATT 
CTTGAAGGAG ATCATGGCTC ACCTGCTGGA GAAATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCC GACTCAGACA TCTGAGATAT ATGATGGTGA TGGTCCCAAA 
AATGTGAGAT CAGATGTTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGA ATGTCCGGTG 
TCTATTAACT TGTCCAAAGC TGAAACTCAG GCTTTGACTA ATTATGGAAG TGGAGAAGAT 
GAGAATGAGG ATGAAGAAAT AGAAGAATTT GAAGAGGGAC CTGTGGATGT CCAGACGTCA 
CTTCAGGCTA ACACTGAAGC TACAGAAGAA ACTGAACACG ATGATCAGGT ACTACAACAT 
GACTTTGAAA AATCAGGAGA AAGCAAAAAT GTCCCATCAG AACAAGATCC CACTACTAGT 
AAAGGCAAGA AATATGACCA AGATAGTACT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAAATGAGC AGCCTTTGAA TAGCGCTGTC CAAAAAGACT CTCTGACTAC CATCGATTCA 
TCTAAACAGC CTAACCCTTT GCCTTTACCT TTACCTGAAA TCGAAACCTT GGTGCCTACA 
GTCAAAGAAG TTAAATCTGC TCAAGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAACAT AAGTCAAAAA 
TCTGATGAAG AAGACTTTGT GAAAGTTGAA GATTTACCAC TTAAACTGAC AATATACTCA 
GAGGCAGATC TAAGGAAGAA AATGGTAGAA GAGCAAGAGA AAAACCATTT ATCTGGTGAA 
ATACTATGTG AAATGCAGAC CGAAGAATTA GCTGGGAATT CTCAGACACT AAAAGAACCT 
GAAACAGTGG GAGCCCAAAG CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630011.1
CDS	616..7002
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629954.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629954.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAGCATGAC 
TTATTAAAAA GGATGTTACA ACAGCAGGAG CAACTGAGAG CTCTGCAGGG AAGACAGGCT 
GCTCTTCTTG CTCTGCAGCA CAAAGCAGAG CAAGCTATTG CTGTGATGGA TGATTCTGAA 
AAGGTTGCAG GGACAACACC TGGCCATCAT ACTGTACCGG GCAGCCAGCC AGCTCGCTCT 
CCTTTTCATC AGAGAGTACC CTTAAGAGTT GTAACAGAAA CTACAGGTAG CGTATCTGGA 
GTTAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCG TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAACTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGGTATC CCAGAGCAGG AATCCATCAG TTTCAGAACA CTTACCTGAT 
GAGAAAGTAC AACTGTTTAG CAAAATGAGA GTATTACAGG AAAAGAAACA AAAAATGGAC 
AAACTACTTG GAGAACTTCA TACACTTCGA GATCAACATC TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CTTCTCCACA AAGAAGTGTG GATCAGAGAA GTACAACTTC AGCACCCTCT 
GCTCCTATAG GCTTAGCACC AGTTGTCAAT GGAGAATCCA ACAGCTTCAC ATCATCTGTT 
CCTTATCCTG TTGCTTCTCT AGTTTCTCAG AATGAGAGTG AAAACGAAGG ACATCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GATTGAATGA ACTAAGAGAA 
CTAGTTCATT ATTACGAACA AACATCAGAT ATGATGACAG ATGCTGTAAA TGAAAACACA 
AAAGATGAAG AAACTGAAGA GTCAGAATAT GATTCTGAAC ATGAAAACCC TGAACCTGTT 
ACTAACATTC GAAATCCTCA AGTAGCTGCC ACTTGGAATG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCTAATAA TAGAGAGGGG AGATCAGTTA ATTCTAATTG TGAAATTAAC 
AACAGATCTG CTGCCAACAT AAGGACTCTG AACATGCCTC CTTCTTTAGC AGATTGTCAC 
TATAATAGAG AAGGAGAACA GGGGATTCAT GGTGCACAAG GTGAAGACGA TGAGGAAGAA 
GAAGAAGCAG AAGATGAAGG AGTCAGTGGG GCTTCATTAA CTAGTCATAG GAGCAGTCTG 
GTTGATGAGG CTGCAGAAGA CGCTGAATTT GAACAGAAGA TCAATAGACT TATGGCTGCC 
AAACAGAAAT TAAGACAGTT ACAAGATCTT GTTGCTATGG TACAGGATGA TGATGCAGCA 
GATCATGGAG TTATCTCTGC CAATACTTCA AATTTGGATG ATTTTTACCC AGCAGAAGAA 
GACAACAAAC AAAGTGCAAA TAATACTAGA GGAAATGCCA ATAAAACACA GAAAGATGCT 
GGAATAAATG AGAAGGCAAG AGAGAAGTTT TATGAGGCTA AACTACAGCA GCAACAGAGA 
GAGCTCAGAC AATTGCAGGA AGAAAGAAAG AAACTGATTG AGATTCAAGA GAAAATTCAA 
GCATTACAGA AGGCATGCCC TGACTTACAG CTGTCAGCCA CTAGTGCGGG TAATTGTCCC 
ACAAAAAAAT ATATCCCAGC TGTTACTTCA ACCCCAGTTG TTAATGGGAA TGAAACCAGT 
ACAAGCAAAT CTGCTTTTGA GCCTGCAGAT CCTTCAGGAG TGGATAATGA GCTGTGGTCA 
GAAATGCGAA GACATGAGAT GTTAAGGGAA GAGCTACGGC AGAGAAGAAA GCAGCTTGAA 
GCACTGATGG CTGAACATCA GAGGAGGCAA GGTCTAGCTG AAACTACATC CCCTCTGGCT 
GTGTCATTGA GAAGTGATGG ATCTGAGAAC CTGTGTACGC CTCAACAGAG TAGAACAGAA 
AAAACCATGG CAACATGGGG AGGTTCTACC CAGTGTGCAC TAGATGAAGA AGGAGATGAA 
GATGGTTACC TTTCTGAAGG AGTTGTTCGG ACAGATGAAG AGGAGGAAGA AGAAGAGCAA 
GATGCAAGTT CGAATGATAA CTTTTCTATG TATCCTCCTA ACAGTGCGAA TCATAACTCT 
TACAACATAA AAGAAACTAA GAATAGGTGG AAGAATAGTC GCCCTTTTAC AGCAGATGGA 
AATTATCGTC CTTTAGCCAA GACAAGACAG CAGAATATCA GCATGCAACG TCAGGAAAAC 
CTTCGTTGGA TGTCCGAACT CTCTTACGTA GAGGAGAAAG AACAATGGCA AGAACAAATT 
AATCAGCTGA AGAAACAACT TGATTTCAGT GTCAATATTT GTCAGACTTT GATGCAAGAC 
CAGCAGACGC TGTCTTGTCT GCTGCAAACT CTTCTCACAG GTCCTTACAG TGTTATGCCC 
AGCAATGTAG CTTCTCCTCA AGTACACCTT ATAATGCACC AGTTGAATCA GTGCTATACT 
CAGCTAACAT GGCAACAGAA TAATGTGCAA AGGTTGAAAC AAATGCTAAA TGAACTTATG 
CGCCAACAGA ATCAGCACCC AGAAAAACCT GGAAGCCAGG AAAGAGGCAG TAGTGCACCA 
CAGCCTTCTT CTCCCAGTTT ATTTTGTCCT TTCAGCTTTC CATCACAGCC TGTAAATCTA 
TTTAACCTAC CCGGGTTTAC TAATTTTTCA TCATTTGCAC CAGGTATGAA TTTTAGCCCT 
TTATTTCCTT CTAATTTTGG AGAATTTTCT CAGAATATTT CTACACCTAC TGAACAGCAG 
CAACCATTAG CCCAGAATTC TTCAGGGAAA ACAGAATATA TGGCTTTTCC AAAACCTTTT 
GAAAGCAGTT CTTCTATTGG AGCAGAAAAA CAAAGGAATC AAAAACAGCC TGGAGAGGAG 
GTGGAAAACA GTAGGACAGC GTGGTTATAT GACCAAGAAG GTGAAGTAGA AAAACCATTT 
ATCAAGACTG GATTTCCAGT GTCTGTAGAG AAAACTACAA ATAGTAATCG CAAAAATCAA 
TTAGATACCG GCCGAAGAAG ACGCCAGTTT GATGAAGAGT CATTAGAAAG CTTTAGCAGT 
ATGCCTGATC CAGTAGATCC GACAACAGTA ACCAAAACAT TCAAGACGAG AAAAGCATCT 
GCACAGGCCA GCCTAGCATC TAAAGATAAA ACTCCTAAAT CAAAGAGTAA GAAAAGGCAT 
TCTGCTCAAC TAAAAAGCAG AGTTAAAAAT ACTGGGTATG AAAGTGCCAG TGTGTCTAGC 
ACATGTGAAC CTTGCAAAAG TAGGAACAGA CATTCAGCCC AGACTGAAGA GCCTGTTCAA 
GCAAAAGTAT TCAGCAGAAA GAATCTTGAG CAGCTGGAAA AAATAATAAA ATATAGTAGG 
TCTACAGAGA TATCTTCAGC ACATGCTAGG AGAATACTAC AGCAGTCTAA CAGAAATGCA 
TGCAATGAAG CACCAGAAAC TGGGAGTGAT TTTTCCATGT TTGAAGCTTT GCGGGATACT 
ATTTATTCTG AAGTAGCTAC ATTAATTTCT CAAAATGAAT CTCATCCACA TTTTCTTATT 
GAACTCTTCC ATGAACTTCA GCTACTTAAT ACAGACTACT TGAGACAAAG GGCTTTATAT 
GCATTGCAGG ACATAGTAAC CAGACATATT TCTGAGAACC ATGAAAAAGA AGGAGAAAAT 
GTAAAGTCAG TGAATTCTGG TACTTGGATA GCATCAAACT CAGAGCTTAC CCCCAGTGAA 
AGCCTTGCTA CCACTGATGA TGAAACTTTT GAGAAGAACT TTGAGAGAGA AACACATAAA 
ATAAGTGAGC AAAATGATGC TGATAATGCT AGCGTCATGT CTGTATCTTC AAATTTTGAG 
CCTTTTGCAA CAGATGATCT AGGTAACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGGGAAT ACGAGCGTAT GAAGACTGAG GCTGAAAGTA GCACAAATAT AAGATGCACC 
TGCAGAATTC TTGAGGATGA GGATGGTGCT GCCGCCACAA GTATGGTTAC TAATTTAGAA 
GAAACTCCTA TTGAAAATCA TGGTTCACAA CAACCTGTAA GTGAAGTTTC TACTGTTCCA 
TGTCCTAGAA TTGATACTCA GCAGCTGGAC CGGCAAATTA AAGCCATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGCTCTT CTCAACTTCT AACTTCAGTA 
AGGCGTATGG TTTTGACCCT CACCCAACAA AATGATGAGA GCAAAGAATT TGTGAAGTTC 
TTTCATAAAC AACTTGGAAG TATATTACAG GATTCACTGG CAAAATTTGC TGGCAGAAAA 
CTGAAAGACT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTGTT TAATGAATTG 
GCTTTCTTTA AGCTCATGCA AGATTTGGAT AATAATAGTA TAACTGTTAA ACAGAAGTGC 
AAAAGGAAAA TAGAAGCAGC TGGAGTGATA CAGTCTTATG CCAAAGAGGC CAAAAGGATT 
CTTGAAGGAG ATCATGGCTC ACCTGCTGGA GAAATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCC GACTCAGACA TCTGAGATAT ATGATGGTGA TGGTCCCAAA 
AATGTGAGAT CAGATGTTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGA ATGTCCGGTG 
TCTATTAACT TGTCCAAAGC TGAAACTCAG GCTTTGACTA ATTATGGAAG TGGAGAAGAT 
GAGAATGAGG ATGAAGAAAT AGAAGAATTT GAAGAGGGAC CTGTGGATGT CCAGACGTCA 
CTTCAGGCTA ACACTGAAGC TACAGAAGAA ACTGAACACG ATGATCAGGT ACTACAACAT 
GACTTTGAAA AATCAGGAGA AAGCAAAAAT GTCCCATCAG AACAAGATCC CACTACTAGT 
AAAGGCAAGA AATATGACCA AGATAGTACT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAAATGAGC AGCCTTTGAA TAGCGCTGTC CAAAAAGACT CTCTGACTAC CATCGATTCA 
TCTAAACAGC CTAACCCTTT GCCTTTACCT TTACCTGAAA TCGAAACCTT GGTGCCTACA 
GTCAAAGAAG TTAAATCTGC TCAAGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAACAT AAGTCAAAAA 
TCTGATGAAG AAGACTTTGT GAAAGTTGAA GATTTACCAC TTAAACTGAC AATATACTCA 
GAGGCAGATC TAAGGAAGAA AATGGTAGAA GAGCAAGAGA AAAACCATTT ATCTGGTGAA 
ATACTATGTG AAATGCAGAC CGAAGAATTA GCTGGGAATT CTCAGACACT AAAAGAACCT 
GAAACAGTGG GAGCCCAAAG CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404151.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259859.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X12, mRNA.

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CloneID	OCe18440
Clone ID Related Accession (Same CDS sequence)	XM_005629954.2 , XM_022404151.1
Accession Version	XM_022404151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAGCATGAC 
TTATTAAAAA GGATGTTACA ACAGCAGGAG CAACTGAGAG CTCTGCAGGG AAGACAGGCT 
GCTCTTCTTG CTCTGCAGCA CAAAGCAGAG CAAGCTATTG CTGTGATGGA TGATTCTGAA 
AAGGTTGCAG GGACAACACC TGGCCATCAT ACTGTACCGG GCAGCCAGCC AGCTCGCTCT 
CCTTTTCATC AGAGAGTACC CTTAAGAGTT GTAACAGAAA CTACAGGTAG CGTATCTGGA 
GTTAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCG TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAACTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGGTATC CCAGAGCAGG AATCCATCAG TTTCAGAACA CTTACCTGAT 
GAGAAAGTAC AACTGTTTAG CAAAATGAGA GTATTACAGG AAAAGAAACA AAAAATGGAC 
AAACTACTTG GAGAACTTCA TACACTTCGA GATCAACATC TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CTTCTCCACA AAGAAGTGTG GATCAGAGAA GTACAACTTC AGCACCCTCT 
GCTCCTATAG GCTTAGCACC AGTTGTCAAT GGAGAATCCA ACAGCTTCAC ATCATCTGTT 
CCTTATCCTG TTGCTTCTCT AGTTTCTCAG AATGAGAGTG AAAACGAAGG ACATCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GATTGAATGA ACTAAGAGAA 
CTAGTTCATT ATTACGAACA AACATCAGAT ATGATGACAG ATGCTGTAAA TGAAAACACA 
AAAGATGAAG AAACTGAAGA GTCAGAATAT GATTCTGAAC ATGAAAACCC TGAACCTGTT 
ACTAACATTC GAAATCCTCA AGTAGCTGCC ACTTGGAATG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCTAATAA TAGAGAGGGG AGATCAGTTA ATTCTAATTG TGAAATTAAC 
AACAGATCTG CTGCCAACAT AAGGACTCTG AACATGCCTC CTTCTTTAGC AGATTGTCAC 
TATAATAGAG AAGGAGAACA GGGGATTCAT GGTGCACAAG GTGAAGACGA TGAGGAAGAA 
GAAGAAGCAG AAGATGAAGG AGTCAGTGGG GCTTCATTAA CTAGTCATAG GAGCAGTCTG 
GTTGATGAGG CTGCAGAAGA CGCTGAATTT GAACAGAAGA TCAATAGACT TATGGCTGCC 
AAACAGAAAT TAAGACAGTT ACAAGATCTT GTTGCTATGG TACAGGATGA TGATGCAGCA 
GATCATGGAG TTATCTCTGC CAATACTTCA AATTTGGATG ATTTTTACCC AGCAGAAGAA 
GACAACAAAC AAAGTGCAAA TAATACTAGA GGAAATGCCA ATAAAACACA GAAAGATGCT 
GGAATAAATG AGAAGGCAAG AGAGAAGTTT TATGAGGCTA AACTACAGCA GCAACAGAGA 
GAGCTCAGAC AATTGCAGGA AGAAAGAAAG AAACTGATTG AGATTCAAGA GAAAATTCAA 
GCATTACAGA AGGCATGCCC TGACTTACAG CTGTCAGCCA CTAGTGCGGG TAATTGTCCC 
ACAAAAAAAT ATATCCCAGC TGTTACTTCA ACCCCAGTTG TTAATGGGAA TGAAACCAGT 
ACAAGCAAAT CTGCTTTTGA GCCTGCAGAT CCTTCAGGAG TGGATAATGA GCTGTGGTCA 
GAAATGCGAA GACATGAGAT GTTAAGGGAA GAGCTACGGC AGAGAAGAAA GCAGCTTGAA 
GCACTGATGG CTGAACATCA GAGGAGGCAA GGTCTAGCTG AAACTACATC CCCTCTGGCT 
GTGTCATTGA GAAGTGATGG ATCTGAGAAC CTGTGTACGC CTCAACAGAG TAGAACAGAA 
AAAACCATGG CAACATGGGG AGGTTCTACC CAGTGTGCAC TAGATGAAGA AGGAGATGAA 
GATGGTTACC TTTCTGAAGG AGTTGTTCGG ACAGATGAAG AGGAGGAAGA AGAAGAGCAA 
GATGCAAGTT CGAATGATAA CTTTTCTATG TATCCTCCTA ACAGTGCGAA TCATAACTCT 
TACAACATAA AAGAAACTAA GAATAGGTGG AAGAATAGTC GCCCTTTTAC AGCAGATGGA 
AATTATCGTC CTTTAGCCAA GACAAGACAG CAGAATATCA GCATGCAACG TCAGGAAAAC 
CTTCGTTGGA TGTCCGAACT CTCTTACGTA GAGGAGAAAG AACAATGGCA AGAACAAATT 
AATCAGCTGA AGAAACAACT TGATTTCAGT GTCAATATTT GTCAGACTTT GATGCAAGAC 
CAGCAGACGC TGTCTTGTCT GCTGCAAACT CTTCTCACAG GTCCTTACAG TGTTATGCCC 
AGCAATGTAG CTTCTCCTCA AGTACACCTT ATAATGCACC AGTTGAATCA GTGCTATACT 
CAGCTAACAT GGCAACAGAA TAATGTGCAA AGGTTGAAAC AAATGCTAAA TGAACTTATG 
CGCCAACAGA ATCAGCACCC AGAAAAACCT GGAAGCCAGG AAAGAGGCAG TAGTGCACCA 
CAGCCTTCTT CTCCCAGTTT ATTTTGTCCT TTCAGCTTTC CATCACAGCC TGTAAATCTA 
TTTAACCTAC CCGGGTTTAC TAATTTTTCA TCATTTGCAC CAGGTATGAA TTTTAGCCCT 
TTATTTCCTT CTAATTTTGG AGAATTTTCT CAGAATATTT CTACACCTAC TGAACAGCAG 
CAACCATTAG CCCAGAATTC TTCAGGGAAA ACAGAATATA TGGCTTTTCC AAAACCTTTT 
GAAAGCAGTT CTTCTATTGG AGCAGAAAAA CAAAGGAATC AAAAACAGCC TGGAGAGGAG 
GTGGAAAACA GTAGGACAGC GTGGTTATAT GACCAAGAAG GTGAAGTAGA AAAACCATTT 
ATCAAGACTG GATTTCCAGT GTCTGTAGAG AAAACTACAA ATAGTAATCG CAAAAATCAA 
TTAGATACCG GCCGAAGAAG ACGCCAGTTT GATGAAGAGT CATTAGAAAG CTTTAGCAGT 
ATGCCTGATC CAGTAGATCC GACAACAGTA ACCAAAACAT TCAAGACGAG AAAAGCATCT 
GCACAGGCCA GCCTAGCATC TAAAGATAAA ACTCCTAAAT CAAAGAGTAA GAAAAGGCAT 
TCTGCTCAAC TAAAAAGCAG AGTTAAAAAT ACTGGGTATG AAAGTGCCAG TGTGTCTAGC 
ACATGTGAAC CTTGCAAAAG TAGGAACAGA CATTCAGCCC AGACTGAAGA GCCTGTTCAA 
GCAAAAGTAT TCAGCAGAAA GAATCTTGAG CAGCTGGAAA AAATAATAAA ATATAGTAGG 
TCTACAGAGA TATCTTCAGC ACATGCTAGG AGAATACTAC AGCAGTCTAA CAGAAATGCA 
TGCAATGAAG CACCAGAAAC TGGGAGTGAT TTTTCCATGT TTGAAGCTTT GCGGGATACT 
ATTTATTCTG AAGTAGCTAC ATTAATTTCT CAAAATGAAT CTCATCCACA TTTTCTTATT 
GAACTCTTCC ATGAACTTCA GCTACTTAAT ACAGACTACT TGAGACAAAG GGCTTTATAT 
GCATTGCAGG ACATAGTAAC CAGACATATT TCTGAGAACC ATGAAAAAGA AGGAGAAAAT 
GTAAAGTCAG TGAATTCTGG TACTTGGATA GCATCAAACT CAGAGCTTAC CCCCAGTGAA 
AGCCTTGCTA CCACTGATGA TGAAACTTTT GAGAAGAACT TTGAGAGAGA AACACATAAA 
ATAAGTGAGC AAAATGATGC TGATAATGCT AGCGTCATGT CTGTATCTTC AAATTTTGAG 
CCTTTTGCAA CAGATGATCT AGGTAACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGGGAAT ACGAGCGTAT GAAGACTGAG GCTGAAAGTA GCACAAATAT AAGATGCACC 
TGCAGAATTC TTGAGGATGA GGATGGTGCT GCCGCCACAA GTATGGTTAC TAATTTAGAA 
GAAACTCCTA TTGAAAATCA TGGTTCACAA CAACCTGTAA GTGAAGTTTC TACTGTTCCA 
TGTCCTAGAA TTGATACTCA GCAGCTGGAC CGGCAAATTA AAGCCATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGCTCTT CTCAACTTCT AACTTCAGTA 
AGGCGTATGG TTTTGACCCT CACCCAACAA AATGATGAGA GCAAAGAATT TGTGAAGTTC 
TTTCATAAAC AACTTGGAAG TATATTACAG GATTCACTGG CAAAATTTGC TGGCAGAAAA 
CTGAAAGACT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTGTT TAATGAATTG 
GCTTTCTTTA AGCTCATGCA AGATTTGGAT AATAATAGTA TAACTGTTAA ACAGAAGTGC 
AAAAGGAAAA TAGAAGCAGC TGGAGTGATA CAGTCTTATG CCAAAGAGGC CAAAAGGATT 
CTTGAAGGAG ATCATGGCTC ACCTGCTGGA GAAATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCC GACTCAGACA TCTGAGATAT ATGATGGTGA TGGTCCCAAA 
AATGTGAGAT CAGATGTTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGA ATGTCCGGTG 
TCTATTAACT TGTCCAAAGC TGAAACTCAG GCTTTGACTA ATTATGGAAG TGGAGAAGAT 
GAGAATGAGG ATGAAGAAAT AGAAGAATTT GAAGAGGGAC CTGTGGATGT CCAGACGTCA 
CTTCAGGCTA ACACTGAAGC TACAGAAGAA ACTGAACACG ATGATCAGGT ACTACAACAT 
GACTTTGAAA AATCAGGAGA AAGCAAAAAT GTCCCATCAG AACAAGATCC CACTACTAGT 
AAAGGCAAGA AATATGACCA AGATAGTACT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAAATGAGC AGCCTTTGAA TAGCGCTGTC CAAAAAGACT CTCTGACTAC CATCGATTCA 
TCTAAACAGC CTAACCCTTT GCCTTTACCT TTACCTGAAA TCGAAACCTT GGTGCCTACA 
GTCAAAGAAG TTAAATCTGC TCAAGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAACAT AAGTCAAAAA 
TCTGATGAAG AAGACTTTGT GAAAGTTGAA GATTTACCAC TTAAACTGAC AATATACTCA 
GAGGCAGATC TAAGGAAGAA AATGGTAGAA GAGCAAGAGA AAAACCATTT ATCTGGTGAA 
ATACTATGTG AAATGCAGAC CGAAGAATTA GCTGGGAATT CTCAGACACT AAAAGAACCT 
GAAACAGTGG GAGCCCAAAG CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259859.1
CDS	426..6812
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404151.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404151.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GCCAGAGATC CTCAGCAGGA GCCTATGGAA GAGATAGAAA ATTTGAAGAA ACAGCATGAC 
TTATTAAAAA GGATGTTACA ACAGCAGGAG CAACTGAGAG CTCTGCAGGG AAGACAGGCT 
GCTCTTCTTG CTCTGCAGCA CAAAGCAGAG CAAGCTATTG CTGTGATGGA TGATTCTGAA 
AAGGTTGCAG GGACAACACC TGGCCATCAT ACTGTACCGG GCAGCCAGCC AGCTCGCTCT 
CCTTTTCATC AGAGAGTACC CTTAAGAGTT GTAACAGAAA CTACAGGTAG CGTATCTGGA 
GTTAGTATCA CCTCTGAACT AAATGAAGAA TTGAATGATT TAATTCAGCG TTTTCATAAT 
CAGCTTCGTG ATTCTCAGCC TCCAACTGTT CCAGACAATA GAAGACAAGC AGAAAGTCTT 
TCATTAACTA GGGAGGTATC CCAGAGCAGG AATCCATCAG TTTCAGAACA CTTACCTGAT 
GAGAAAGTAC AACTGTTTAG CAAAATGAGA GTATTACAGG AAAAGAAACA AAAAATGGAC 
AAACTACTTG GAGAACTTCA TACACTTCGA GATCAACATC TGAACAATTC ATCATTTGTG 
CCTTCTTCAG CTTCTCCACA AAGAAGTGTG GATCAGAGAA GTACAACTTC AGCACCCTCT 
GCTCCTATAG GCTTAGCACC AGTTGTCAAT GGAGAATCCA ACAGCTTCAC ATCATCTGTT 
CCTTATCCTG TTGCTTCTCT AGTTTCTCAG AATGAGAGTG AAAACGAAGG ACATCTAAAT 
CCAACTGAAA AACTCCAGAA GTTAAATGAA GTTCGAAAGA GATTGAATGA ACTAAGAGAA 
CTAGTTCATT ATTACGAACA AACATCAGAT ATGATGACAG ATGCTGTAAA TGAAAACACA 
AAAGATGAAG AAACTGAAGA GTCAGAATAT GATTCTGAAC ATGAAAACCC TGAACCTGTT 
ACTAACATTC GAAATCCTCA AGTAGCTGCC ACTTGGAATG AAGTAAATAG TAATTCTAAT 
GCACAGTGTG TTTCTAATAA TAGAGAGGGG AGATCAGTTA ATTCTAATTG TGAAATTAAC 
AACAGATCTG CTGCCAACAT AAGGACTCTG AACATGCCTC CTTCTTTAGC AGATTGTCAC 
TATAATAGAG AAGGAGAACA GGGGATTCAT GGTGCACAAG GTGAAGACGA TGAGGAAGAA 
GAAGAAGCAG AAGATGAAGG AGTCAGTGGG GCTTCATTAA CTAGTCATAG GAGCAGTCTG 
GTTGATGAGG CTGCAGAAGA CGCTGAATTT GAACAGAAGA TCAATAGACT TATGGCTGCC 
AAACAGAAAT TAAGACAGTT ACAAGATCTT GTTGCTATGG TACAGGATGA TGATGCAGCA 
GATCATGGAG TTATCTCTGC CAATACTTCA AATTTGGATG ATTTTTACCC AGCAGAAGAA 
GACAACAAAC AAAGTGCAAA TAATACTAGA GGAAATGCCA ATAAAACACA GAAAGATGCT 
GGAATAAATG AGAAGGCAAG AGAGAAGTTT TATGAGGCTA AACTACAGCA GCAACAGAGA 
GAGCTCAGAC AATTGCAGGA AGAAAGAAAG AAACTGATTG AGATTCAAGA GAAAATTCAA 
GCATTACAGA AGGCATGCCC TGACTTACAG CTGTCAGCCA CTAGTGCGGG TAATTGTCCC 
ACAAAAAAAT ATATCCCAGC TGTTACTTCA ACCCCAGTTG TTAATGGGAA TGAAACCAGT 
ACAAGCAAAT CTGCTTTTGA GCCTGCAGAT CCTTCAGGAG TGGATAATGA GCTGTGGTCA 
GAAATGCGAA GACATGAGAT GTTAAGGGAA GAGCTACGGC AGAGAAGAAA GCAGCTTGAA 
GCACTGATGG CTGAACATCA GAGGAGGCAA GGTCTAGCTG AAACTACATC CCCTCTGGCT 
GTGTCATTGA GAAGTGATGG ATCTGAGAAC CTGTGTACGC CTCAACAGAG TAGAACAGAA 
AAAACCATGG CAACATGGGG AGGTTCTACC CAGTGTGCAC TAGATGAAGA AGGAGATGAA 
GATGGTTACC TTTCTGAAGG AGTTGTTCGG ACAGATGAAG AGGAGGAAGA AGAAGAGCAA 
GATGCAAGTT CGAATGATAA CTTTTCTATG TATCCTCCTA ACAGTGCGAA TCATAACTCT 
TACAACATAA AAGAAACTAA GAATAGGTGG AAGAATAGTC GCCCTTTTAC AGCAGATGGA 
AATTATCGTC CTTTAGCCAA GACAAGACAG CAGAATATCA GCATGCAACG TCAGGAAAAC 
CTTCGTTGGA TGTCCGAACT CTCTTACGTA GAGGAGAAAG AACAATGGCA AGAACAAATT 
AATCAGCTGA AGAAACAACT TGATTTCAGT GTCAATATTT GTCAGACTTT GATGCAAGAC 
CAGCAGACGC TGTCTTGTCT GCTGCAAACT CTTCTCACAG GTCCTTACAG TGTTATGCCC 
AGCAATGTAG CTTCTCCTCA AGTACACCTT ATAATGCACC AGTTGAATCA GTGCTATACT 
CAGCTAACAT GGCAACAGAA TAATGTGCAA AGGTTGAAAC AAATGCTAAA TGAACTTATG 
CGCCAACAGA ATCAGCACCC AGAAAAACCT GGAAGCCAGG AAAGAGGCAG TAGTGCACCA 
CAGCCTTCTT CTCCCAGTTT ATTTTGTCCT TTCAGCTTTC CATCACAGCC TGTAAATCTA 
TTTAACCTAC CCGGGTTTAC TAATTTTTCA TCATTTGCAC CAGGTATGAA TTTTAGCCCT 
TTATTTCCTT CTAATTTTGG AGAATTTTCT CAGAATATTT CTACACCTAC TGAACAGCAG 
CAACCATTAG CCCAGAATTC TTCAGGGAAA ACAGAATATA TGGCTTTTCC AAAACCTTTT 
GAAAGCAGTT CTTCTATTGG AGCAGAAAAA CAAAGGAATC AAAAACAGCC TGGAGAGGAG 
GTGGAAAACA GTAGGACAGC GTGGTTATAT GACCAAGAAG GTGAAGTAGA AAAACCATTT 
ATCAAGACTG GATTTCCAGT GTCTGTAGAG AAAACTACAA ATAGTAATCG CAAAAATCAA 
TTAGATACCG GCCGAAGAAG ACGCCAGTTT GATGAAGAGT CATTAGAAAG CTTTAGCAGT 
ATGCCTGATC CAGTAGATCC GACAACAGTA ACCAAAACAT TCAAGACGAG AAAAGCATCT 
GCACAGGCCA GCCTAGCATC TAAAGATAAA ACTCCTAAAT CAAAGAGTAA GAAAAGGCAT 
TCTGCTCAAC TAAAAAGCAG AGTTAAAAAT ACTGGGTATG AAAGTGCCAG TGTGTCTAGC 
ACATGTGAAC CTTGCAAAAG TAGGAACAGA CATTCAGCCC AGACTGAAGA GCCTGTTCAA 
GCAAAAGTAT TCAGCAGAAA GAATCTTGAG CAGCTGGAAA AAATAATAAA ATATAGTAGG 
TCTACAGAGA TATCTTCAGC ACATGCTAGG AGAATACTAC AGCAGTCTAA CAGAAATGCA 
TGCAATGAAG CACCAGAAAC TGGGAGTGAT TTTTCCATGT TTGAAGCTTT GCGGGATACT 
ATTTATTCTG AAGTAGCTAC ATTAATTTCT CAAAATGAAT CTCATCCACA TTTTCTTATT 
GAACTCTTCC ATGAACTTCA GCTACTTAAT ACAGACTACT TGAGACAAAG GGCTTTATAT 
GCATTGCAGG ACATAGTAAC CAGACATATT TCTGAGAACC ATGAAAAAGA AGGAGAAAAT 
GTAAAGTCAG TGAATTCTGG TACTTGGATA GCATCAAACT CAGAGCTTAC CCCCAGTGAA 
AGCCTTGCTA CCACTGATGA TGAAACTTTT GAGAAGAACT TTGAGAGAGA AACACATAAA 
ATAAGTGAGC AAAATGATGC TGATAATGCT AGCGTCATGT CTGTATCTTC AAATTTTGAG 
CCTTTTGCAA CAGATGATCT AGGTAACACT GTGATTCACT TAGATCAGGC ATTAGCCAGA 
ATGAGGGAAT ACGAGCGTAT GAAGACTGAG GCTGAAAGTA GCACAAATAT AAGATGCACC 
TGCAGAATTC TTGAGGATGA GGATGGTGCT GCCGCCACAA GTATGGTTAC TAATTTAGAA 
GAAACTCCTA TTGAAAATCA TGGTTCACAA CAACCTGTAA GTGAAGTTTC TACTGTTCCA 
TGTCCTAGAA TTGATACTCA GCAGCTGGAC CGGCAAATTA AAGCCATTAT GAAAGAAGTC 
ATTCCTTTTT TGAAGGAGCA CATGGATGAA GTATGCTCTT CTCAACTTCT AACTTCAGTA 
AGGCGTATGG TTTTGACCCT CACCCAACAA AATGATGAGA GCAAAGAATT TGTGAAGTTC 
TTTCATAAAC AACTTGGAAG TATATTACAG GATTCACTGG CAAAATTTGC TGGCAGAAAA 
CTGAAAGACT GTGGAGAAGA TCTTCTTGTA GAGATATCTG AAGTGTTGTT TAATGAATTG 
GCTTTCTTTA AGCTCATGCA AGATTTGGAT AATAATAGTA TAACTGTTAA ACAGAAGTGC 
AAAAGGAAAA TAGAAGCAGC TGGAGTGATA CAGTCTTATG CCAAAGAGGC CAAAAGGATT 
CTTGAAGGAG ATCATGGCTC ACCTGCTGGA GAAATTGATG ATGAAGACAA AGACAAGGAT 
GAAACTGAAA CAGTTAAGCC GACTCAGACA TCTGAGATAT ATGATGGTGA TGGTCCCAAA 
AATGTGAGAT CAGATGTTTC TGATCAAGAG GAAGATGAAG AAAGTGAAGA ATGTCCGGTG 
TCTATTAACT TGTCCAAAGC TGAAACTCAG GCTTTGACTA ATTATGGAAG TGGAGAAGAT 
GAGAATGAGG ATGAAGAAAT AGAAGAATTT GAAGAGGGAC CTGTGGATGT CCAGACGTCA 
CTTCAGGCTA ACACTGAAGC TACAGAAGAA ACTGAACACG ATGATCAGGT ACTACAACAT 
GACTTTGAAA AATCAGGAGA AAGCAAAAAT GTCCCATCAG AACAAGATCC CACTACTAGT 
AAAGGCAAGA AATATGACCA AGATAGTACT CCTGTGAAAC CCTGTTACCT CAATATCTTG 
GAAAATGAGC AGCCTTTGAA TAGCGCTGTC CAAAAAGACT CTCTGACTAC CATCGATTCA 
TCTAAACAGC CTAACCCTTT GCCTTTACCT TTACCTGAAA TCGAAACCTT GGTGCCTACA 
GTCAAAGAAG TTAAATCTGC TCAAGAAACT CCTGAAAGCT CTCTGGCTGG AAGTCCTGAT 
ACTGAATCTC CAGTGTTAGT GAATGACTAT GAAGCAGAAT CTGGTAACAT AAGTCAAAAA 
TCTGATGAAG AAGACTTTGT GAAAGTTGAA GATTTACCAC TTAAACTGAC AATATACTCA 
GAGGCAGATC TAAGGAAGAA AATGGTAGAA GAGCAAGAGA AAAACCATTT ATCTGGTGAA 
ATACTATGTG AAATGCAGAC CGAAGAATTA GCTGGGAATT CTCAGACACT AAAAGAACCT 
GAAACAGTGG GAGCCCAAAG CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629955.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630012.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X14, mRNA.

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CloneID	OCe18441
Clone ID Related Accession (Same CDS sequence)	XM_005629955.1 , XM_022404152.1
Accession Version	XM_005629955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG GGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC ATCCACATTT TCTTATTGAA CTCTTCCATG AACTTCAGCT ACTTAATACA 
GACTACTTGA GACAAAGGGC TTTATATGCA TTGCAGGACA TAGTAACCAG ACATATTTCT 
GAGAACCATG AAAAAGAAGG AGAAAATGTA AAGTCAGTGA ATTCTGGTAC TTGGATAGCA 
TCAAACTCAG AGCTTACCCC CAGTGAAAGC CTTGCTACCA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AGAGAGAAAC ACATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGC 
GTCATGTCTG TATCTTCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACTGTG 
ATTCACTTAG ATCAGGCATT AGCCAGAATG AGGGAATACG AGCGTATGAA GACTGAGGCT 
GAAAGTAGCA CAAATATAAG ATGCACCTGC AGAATTCTTG AGGATGAGGA TGGTGCTGCC 
GCCACAAGTA TGGTTACTAA TTTAGAAGAA ACTCCTATTG AAAATCATGG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC TGTTCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CCATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCTTCTC AACTTCTAAC TTCAGTAAGG CGTATGGTTT TGACCCTCAC CCAACAAAAT 
GATGAGAGCA AAGAATTTGT GAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTTAA TGAATTGGCT TTCTTTAAGC TCATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAAGTGCAAA AGGAAAATAG AAGCAGCTGG AGTGATACAG 
TCTTATGCCA AAGAGGCCAA AAGGATTCTT GAAGGAGATC ATGGCTCACC TGCTGGAGAA 
ATTGATGATG AAGACAAAGA CAAGGATGAA ACTGAAACAG TTAAGCCGAC TCAGACATCT 
GAGATATATG ATGGTGATGG TCCCAAAAAT GTGAGATCAG ATGTTTCTGA TCAAGAGGAA 
GATGAAGAAA GTGAAGAATG TCCGGTGTCT ATTAACTTGT CCAAAGCTGA AACTCAGGCT 
TTGACTAATT ATGGAAGTGG AGAAGATGAG AATGAGGATG AAGAAATAGA AGAATTTGAA 
GAGGGACCTG TGGATGTCCA GACGTCACTT CAGGCTAACA CTGAAGCTAC AGAAGAAACT 
GAACACGATG ATCAGGTACT ACAACATGAC TTTGAAAAAT CAGGAGAAAG CAAAAATGTC 
CCATCAGAAC AAGATCCCAC TACTAGTAAA GGCAAGAAAT ATGACCAAGA TAGTACTCCT 
GTGAAACCCT GTTACCTCAA TATCTTGGAA AATGAGCAGC CTTTGAATAG CGCTGTCCAA 
AAAGACTCTC TGACTACCAT CGATTCATCT AAACAGCCTA ACCCTTTGCC TTTACCTTTA 
CCTGAAATCG AAACCTTGGT GCCTACAGTC AAAGAAGTTA AATCTGCTCA AGAAACTCCT 
GAAAGCTCTC TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA 
GCAGAATCTG GTAACATAAG TCAAAAATCT GATGAAGAAG ACTTTGTGAA AGTTGAAGAT 
TTACCACTTA AACTGACAAT ATACTCAGAG GCAGATCTAA GGAAGAAAAT GGTAGAAGAG 
CAAGAGAAAA ACCATTTATC TGGTGAAATA CTATGTGAAA TGCAGACCGA AGAATTAGCT 
GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGCGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630012.1
CDS	616..6969
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629955.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629955.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG GGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC ATCCACATTT TCTTATTGAA CTCTTCCATG AACTTCAGCT ACTTAATACA 
GACTACTTGA GACAAAGGGC TTTATATGCA TTGCAGGACA TAGTAACCAG ACATATTTCT 
GAGAACCATG AAAAAGAAGG AGAAAATGTA AAGTCAGTGA ATTCTGGTAC TTGGATAGCA 
TCAAACTCAG AGCTTACCCC CAGTGAAAGC CTTGCTACCA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AGAGAGAAAC ACATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGC 
GTCATGTCTG TATCTTCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACTGTG 
ATTCACTTAG ATCAGGCATT AGCCAGAATG AGGGAATACG AGCGTATGAA GACTGAGGCT 
GAAAGTAGCA CAAATATAAG ATGCACCTGC AGAATTCTTG AGGATGAGGA TGGTGCTGCC 
GCCACAAGTA TGGTTACTAA TTTAGAAGAA ACTCCTATTG AAAATCATGG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC TGTTCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CCATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCTTCTC AACTTCTAAC TTCAGTAAGG CGTATGGTTT TGACCCTCAC CCAACAAAAT 
GATGAGAGCA AAGAATTTGT GAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTTAA TGAATTGGCT TTCTTTAAGC TCATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAAGTGCAAA AGGAAAATAG AAGCAGCTGG AGTGATACAG 
TCTTATGCCA AAGAGGCCAA AAGGATTCTT GAAGGAGATC ATGGCTCACC TGCTGGAGAA 
ATTGATGATG AAGACAAAGA CAAGGATGAA ACTGAAACAG TTAAGCCGAC TCAGACATCT 
GAGATATATG ATGGTGATGG TCCCAAAAAT GTGAGATCAG ATGTTTCTGA TCAAGAGGAA 
GATGAAGAAA GTGAAGAATG TCCGGTGTCT ATTAACTTGT CCAAAGCTGA AACTCAGGCT 
TTGACTAATT ATGGAAGTGG AGAAGATGAG AATGAGGATG AAGAAATAGA AGAATTTGAA 
GAGGGACCTG TGGATGTCCA GACGTCACTT CAGGCTAACA CTGAAGCTAC AGAAGAAACT 
GAACACGATG ATCAGGTACT ACAACATGAC TTTGAAAAAT CAGGAGAAAG CAAAAATGTC 
CCATCAGAAC AAGATCCCAC TACTAGTAAA GGCAAGAAAT ATGACCAAGA TAGTACTCCT 
GTGAAACCCT GTTACCTCAA TATCTTGGAA AATGAGCAGC CTTTGAATAG CGCTGTCCAA 
AAAGACTCTC TGACTACCAT CGATTCATCT AAACAGCCTA ACCCTTTGCC TTTACCTTTA 
CCTGAAATCG AAACCTTGGT GCCTACAGTC AAAGAAGTTA AATCTGCTCA AGAAACTCCT 
GAAAGCTCTC TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA 
GCAGAATCTG GTAACATAAG TCAAAAATCT GATGAAGAAG ACTTTGTGAA AGTTGAAGAT 
TTACCACTTA AACTGACAAT ATACTCAGAG GCAGATCTAA GGAAGAAAAT GGTAGAAGAG 
CAAGAGAAAA ACCATTTATC TGGTGAAATA CTATGTGAAA TGCAGACCGA AGAATTAGCT 
GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGCGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404152.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259860.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X13, mRNA.

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CloneID	OCe18441
Clone ID Related Accession (Same CDS sequence)	XM_005629955.1 , XM_022404152.1
Accession Version	XM_022404152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG GGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC ATCCACATTT TCTTATTGAA CTCTTCCATG AACTTCAGCT ACTTAATACA 
GACTACTTGA GACAAAGGGC TTTATATGCA TTGCAGGACA TAGTAACCAG ACATATTTCT 
GAGAACCATG AAAAAGAAGG AGAAAATGTA AAGTCAGTGA ATTCTGGTAC TTGGATAGCA 
TCAAACTCAG AGCTTACCCC CAGTGAAAGC CTTGCTACCA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AGAGAGAAAC ACATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGC 
GTCATGTCTG TATCTTCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACTGTG 
ATTCACTTAG ATCAGGCATT AGCCAGAATG AGGGAATACG AGCGTATGAA GACTGAGGCT 
GAAAGTAGCA CAAATATAAG ATGCACCTGC AGAATTCTTG AGGATGAGGA TGGTGCTGCC 
GCCACAAGTA TGGTTACTAA TTTAGAAGAA ACTCCTATTG AAAATCATGG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC TGTTCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CCATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCTTCTC AACTTCTAAC TTCAGTAAGG CGTATGGTTT TGACCCTCAC CCAACAAAAT 
GATGAGAGCA AAGAATTTGT GAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTTAA TGAATTGGCT TTCTTTAAGC TCATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAAGTGCAAA AGGAAAATAG AAGCAGCTGG AGTGATACAG 
TCTTATGCCA AAGAGGCCAA AAGGATTCTT GAAGGAGATC ATGGCTCACC TGCTGGAGAA 
ATTGATGATG AAGACAAAGA CAAGGATGAA ACTGAAACAG TTAAGCCGAC TCAGACATCT 
GAGATATATG ATGGTGATGG TCCCAAAAAT GTGAGATCAG ATGTTTCTGA TCAAGAGGAA 
GATGAAGAAA GTGAAGAATG TCCGGTGTCT ATTAACTTGT CCAAAGCTGA AACTCAGGCT 
TTGACTAATT ATGGAAGTGG AGAAGATGAG AATGAGGATG AAGAAATAGA AGAATTTGAA 
GAGGGACCTG TGGATGTCCA GACGTCACTT CAGGCTAACA CTGAAGCTAC AGAAGAAACT 
GAACACGATG ATCAGGTACT ACAACATGAC TTTGAAAAAT CAGGAGAAAG CAAAAATGTC 
CCATCAGAAC AAGATCCCAC TACTAGTAAA GGCAAGAAAT ATGACCAAGA TAGTACTCCT 
GTGAAACCCT GTTACCTCAA TATCTTGGAA AATGAGCAGC CTTTGAATAG CGCTGTCCAA 
AAAGACTCTC TGACTACCAT CGATTCATCT AAACAGCCTA ACCCTTTGCC TTTACCTTTA 
CCTGAAATCG AAACCTTGGT GCCTACAGTC AAAGAAGTTA AATCTGCTCA AGAAACTCCT 
GAAAGCTCTC TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA 
GCAGAATCTG GTAACATAAG TCAAAAATCT GATGAAGAAG ACTTTGTGAA AGTTGAAGAT 
TTACCACTTA AACTGACAAT ATACTCAGAG GCAGATCTAA GGAAGAAAAT GGTAGAAGAG 
CAAGAGAAAA ACCATTTATC TGGTGAAATA CTATGTGAAA TGCAGACCGA AGAATTAGCT 
GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGCGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259860.1
CDS	426..6779
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTEESFSLRIRPCIEDKLGNSASQEQVSDIDVTTSPKGKGDRPPQSDRELRPDRKYNRKRGFPSKARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404152.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404152.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCTAGAG AAAATGAGGA GGAGGATGTT CGGACTATAG ATTCAGCTGT GGGATCTGGT 
TCTGTAGCTG AGAGCACATC GCTAAACATA GATGTGCAGT CTGAGGCTTC AGATACCACG 
GAGGAATCTT TTAGTCTGAG AATTCGGCCG TGCATTGAGG ACAAACTAGG GAATTCAGCT 
TCACAAGAAC AGGTTTCAGA CATTGACGTT ACCACAAGTC CAAAAGGGAA AGGTGACAGA 
CCTCCTCAGA GTGACAGGGA ACTGAGGCCT GATAGGAAAT ATAACCGAAA ACGTGGATTT 
CCCTCAAAGG CCAGAGATCC TCAGCAGGAG CCTATGGAAG AGATAGAAAA TTTGAAGAAA 
CAGCATGACT TATTAAAAAG GATGTTACAA CAGCAGGAGC AACTGAGAGC TCTGCAGGGA 
AGACAGGCTG CTCTTCTTGC TCTGCAGCAC AAAGCAGAGC AAGCTATTGC TGTGATGGAT 
GATTCTGAAA AGGTTGCAGG GACAACACCT GGCCATCATA CTGTACCGGG CAGCCAGCCA 
GCTCGCTCTC CTTTTCATCA GAGAGTACCC TTAAGAGTTG TAACAGAAAC TACAGGTAGC 
GTATCTGGAG TTAGTATCAC CTCTGAACTA AATGAAGAAT TGAATGATTT AATTCAGCGT 
TTTCATAATC AGCTTCGTGA TTCTCAGCCT CCAACTGTTC CAGACAATAG AAGACAAGCA 
GAAAGTCTTT CATTAACTAG GGAGGTATCC CAGAGCAGGA ATCCATCAGT TTCAGAACAC 
TTACCTGATG AGAAAGTACA ACTGTTTAGC AAAATGAGAG TATTACAGGA AAAGAAACAA 
AAAATGGACA AACTACTTGG AGAACTTCAT ACACTTCGAG ATCAACATCT GAACAATTCA 
TCATTTGTGC CTTCTTCAGC TTCTCCACAA AGAAGTGTGG ATCAGAGAAG TACAACTTCA 
GCACCCTCTG CTCCTATAGG CTTAGCACCA GTTGTCAATG GAGAATCCAA CAGCTTCACA 
TCATCTGTTC CTTATCCTGT TGCTTCTCTA GTTTCTCAGA ATGAGAGTGA AAACGAAGGA 
CATCTAAATC CAACTGAAAA ACTCCAGAAG TTAAATGAAG TTCGAAAGAG ATTGAATGAA 
CTAAGAGAAC TAGTTCATTA TTACGAACAA ACATCAGATA TGATGACAGA TGCTGTAAAT 
GAAAACACAA AAGATGAAGA AACTGAAGAG TCAGAATATG ATTCTGAACA TGAAAACCCT 
GAACCTGTTA CTAACATTCG AAATCCTCAA GTAGCTGCCA CTTGGAATGA AGTAAATAGT 
AATTCTAATG CACAGTGTGT TTCTAATAAT AGAGAGGGGA GATCAGTTAA TTCTAATTGT 
GAAATTAACA ACAGATCTGC TGCCAACATA AGGACTCTGA ACATGCCTCC TTCTTTAGCA 
GATTGTCACT ATAATAGAGA AGGAGAACAG GGGATTCATG GTGCACAAGG TGAAGACGAT 
GAGGAAGAAG AAGAAGCAGA AGATGAAGGA GTCAGTGGGG CTTCATTAAC TAGTCATAGG 
AGCAGTCTGG TTGATGAGGC TGCAGAAGAC GCTGAATTTG AACAGAAGAT CAATAGACTT 
ATGGCTGCCA AACAGAAATT AAGACAGTTA CAAGATCTTG TTGCTATGGT ACAGGATGAT 
GATGCAGCAG ATCATGGAGT TATCTCTGCC AATACTTCAA ATTTGGATGA TTTTTACCCA 
GCAGAAGAAG ACAACAAACA AAGTGCAAAT AATACTAGAG GAAATGCCAA TAAAACACAG 
AAAGATGCTG GAATAAATGA GAAGGCAAGA GAGAAGTTTT ATGAGGCTAA ACTACAGCAG 
CAACAGAGAG AGCTCAGACA ATTGCAGGAA GAAAGAAAGA AACTGATTGA GATTCAAGAG 
AAAATTCAAG CATTACAGAA GGCATGCCCT GACTTACAGC TGTCAGCCAC TAGTGCGGGT 
AATTGTCCCA CAAAAAAATA TATCCCAGCT GTTACTTCAA CCCCAGTTGT TAATGGGAAT 
GAAACCAGTA CAAGCAAATC TGCTTTTGAG CCTGCAGATC CTTCAGGAGT GGATAATGAG 
CTGTGGTCAG AAATGCGAAG ACATGAGATG TTAAGGGAAG AGCTACGGCA GAGAAGAAAG 
CAGCTTGAAG CACTGATGGC TGAACATCAG AGGAGGCAAG GTCTAGCTGA AACTACATCC 
CCTCTGGCTG TGTCATTGAG AAGTGATGGA TCTGAGAACC TGTGTACGCC TCAACAGAGT 
AGAACAGAAA AAACCATGGC AACATGGGGA GGTTCTACCC AGTGTGCACT AGATGAAGAA 
GGAGATGAAG ATGGTTACCT TTCTGAAGGA GTTGTTCGGA CAGATGAAGA GGAGGAAGAA 
GAAGAGCAAG ATGCAAGTTC GAATGATAAC TTTTCTATGT ATCCTCCTAA CAGTGCGAAT 
CATAACTCTT ACAACATAAA AGAAACTAAG AATAGGTGGA AGAATAGTCG CCCTTTTACA 
GCAGATGGAA ATTATCGTCC TTTAGCCAAG ACAAGACAGC AGAATATCAG CATGCAACGT 
CAGGAAAACC TTCGTTGGAT GTCCGAACTC TCTTACGTAG AGGAGAAAGA ACAATGGCAA 
GAACAAATTA ATCAGCTGAA GAAACAACTT GATTTCAGTG TCAATATTTG TCAGACTTTG 
ATGCAAGACC AGCAGACGCT GTCTTGTCTG CTGCAAACTC TTCTCACAGG TCCTTACAGT 
GTTATGCCCA GCAATGTAGC TTCTCCTCAA GTACACCTTA TAATGCACCA GTTGAATCAG 
TGCTATACTC AGCTAACATG GCAACAGAAT AATGTGCAAA GGTTGAAACA AATGCTAAAT 
GAACTTATGC GCCAACAGAA TCAGCACCCA GAAAAACCTG GAAGCCAGGA AAGAGGCAGT 
AGTGCACCAC AGCCTTCTTC TCCCAGTTTA TTTTGTCCTT TCAGCTTTCC ATCACAGCCT 
GTAAATCTAT TTAACCTACC CGGGTTTACT AATTTTTCAT CATTTGCACC AGGTATGAAT 
TTTAGCCCTT TATTTCCTTC TAATTTTGGA GAATTTTCTC AGAATATTTC TACACCTACT 
GAACAGCAGC AACCATTAGC CCAGAATTCT TCAGGGAAAA CAGAATATAT GGCTTTTCCA 
AAACCTTTTG AAAGCAGTTC TTCTATTGGA GCAGAAAAAC AAAGGAATCA AAAACAGCCT 
GGAGAGGAGG TGGAAAACAG TAGGACAGCG TGGTTATATG ACCAAGAAGG TGAAGTAGAA 
AAACCATTTA TCAAGACTGG ATTTCCAGTG TCTGTAGAGA AAACTACAAA TAGTAATCGC 
AAAAATCAAT TAGATACCGG CCGAAGAAGA CGCCAGTTTG ATGAAGAGTC ATTAGAAAGC 
TTTAGCAGTA TGCCTGATCC AGTAGATCCG ACAACAGTAA CCAAAACATT CAAGACGAGA 
AAAGCATCTG CACAGGCCAG CCTAGCATCT AAAGATAAAA CTCCTAAATC AAAGAGTAAG 
AAAAGGCATT CTGCTCAACT AAAAAGCAGA GTTAAAAATA CTGAAACTGG GAGTGATTTT 
TCCATGTTTG AAGCTTTGCG GGATACTATT TATTCTGAAG TAGCTACATT AATTTCTCAA 
AATGAATCTC ATCCACATTT TCTTATTGAA CTCTTCCATG AACTTCAGCT ACTTAATACA 
GACTACTTGA GACAAAGGGC TTTATATGCA TTGCAGGACA TAGTAACCAG ACATATTTCT 
GAGAACCATG AAAAAGAAGG AGAAAATGTA AAGTCAGTGA ATTCTGGTAC TTGGATAGCA 
TCAAACTCAG AGCTTACCCC CAGTGAAAGC CTTGCTACCA CTGATGATGA AACTTTTGAG 
AAGAACTTTG AGAGAGAAAC ACATAAAATA AGTGAGCAAA ATGATGCTGA TAATGCTAGC 
GTCATGTCTG TATCTTCAAA TTTTGAGCCT TTTGCAACAG ATGATCTAGG TAACACTGTG 
ATTCACTTAG ATCAGGCATT AGCCAGAATG AGGGAATACG AGCGTATGAA GACTGAGGCT 
GAAAGTAGCA CAAATATAAG ATGCACCTGC AGAATTCTTG AGGATGAGGA TGGTGCTGCC 
GCCACAAGTA TGGTTACTAA TTTAGAAGAA ACTCCTATTG AAAATCATGG TTCACAACAA 
CCTGTAAGTG AAGTTTCTAC TGTTCCATGT CCTAGAATTG ATACTCAGCA GCTGGACCGG 
CAAATTAAAG CCATTATGAA AGAAGTCATT CCTTTTTTGA AGGAGCACAT GGATGAAGTA 
TGCTCTTCTC AACTTCTAAC TTCAGTAAGG CGTATGGTTT TGACCCTCAC CCAACAAAAT 
GATGAGAGCA AAGAATTTGT GAAGTTCTTT CATAAACAAC TTGGAAGTAT ATTACAGGAT 
TCACTGGCAA AATTTGCTGG CAGAAAACTG AAAGACTGTG GAGAAGATCT TCTTGTAGAG 
ATATCTGAAG TGTTGTTTAA TGAATTGGCT TTCTTTAAGC TCATGCAAGA TTTGGATAAT 
AATAGTATAA CTGTTAAACA GAAGTGCAAA AGGAAAATAG AAGCAGCTGG AGTGATACAG 
TCTTATGCCA AAGAGGCCAA AAGGATTCTT GAAGGAGATC ATGGCTCACC TGCTGGAGAA 
ATTGATGATG AAGACAAAGA CAAGGATGAA ACTGAAACAG TTAAGCCGAC TCAGACATCT 
GAGATATATG ATGGTGATGG TCCCAAAAAT GTGAGATCAG ATGTTTCTGA TCAAGAGGAA 
GATGAAGAAA GTGAAGAATG TCCGGTGTCT ATTAACTTGT CCAAAGCTGA AACTCAGGCT 
TTGACTAATT ATGGAAGTGG AGAAGATGAG AATGAGGATG AAGAAATAGA AGAATTTGAA 
GAGGGACCTG TGGATGTCCA GACGTCACTT CAGGCTAACA CTGAAGCTAC AGAAGAAACT 
GAACACGATG ATCAGGTACT ACAACATGAC TTTGAAAAAT CAGGAGAAAG CAAAAATGTC 
CCATCAGAAC AAGATCCCAC TACTAGTAAA GGCAAGAAAT ATGACCAAGA TAGTACTCCT 
GTGAAACCCT GTTACCTCAA TATCTTGGAA AATGAGCAGC CTTTGAATAG CGCTGTCCAA 
AAAGACTCTC TGACTACCAT CGATTCATCT AAACAGCCTA ACCCTTTGCC TTTACCTTTA 
CCTGAAATCG AAACCTTGGT GCCTACAGTC AAAGAAGTTA AATCTGCTCA AGAAACTCCT 
GAAAGCTCTC TGGCTGGAAG TCCTGATACT GAATCTCCAG TGTTAGTGAA TGACTATGAA 
GCAGAATCTG GTAACATAAG TCAAAAATCT GATGAAGAAG ACTTTGTGAA AGTTGAAGAT 
TTACCACTTA AACTGACAAT ATACTCAGAG GCAGATCTAA GGAAGAAAAT GGTAGAAGAG 
CAAGAGAAAA ACCATTTATC TGGTGAAATA CTATGTGAAA TGCAGACCGA AGAATTAGCT 
GGGAATTCTC AGACACTAAA AGAACCTGAA ACAGTGGGAG CCCAAAGCGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_005629956.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630013.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X15, mRNA.

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CloneID	OCe18442
Clone ID Related Accession (Same CDS sequence)	XM_005629956.2 , XM_022404155.1
Accession Version	XM_005629956.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pericentriolar material 1 protein isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GAAAAGGTTG CAGGGACAAC ACCTGGCCAT CATACTGTAC CGGGCAGCCA GCCAGCTCGC 
TCTCCTTTTC ATCAGAGAGT ACCCTTAAGA GTTGTAACAG AAACTACAGG TAGCGTATCT 
GGAGTTAGTA TCACCTCTGA ACTAAATGAA GAATTGAATG ATTTAATTCA GCGTTTTCAT 
AATCAGCTTC GTGATTCTCA GCCTCCAACT GTTCCAGACA ATAGAAGACA AGCAGAAAGT 
CTTTCATTAA CTAGGGAGGT ATCCCAGAGC AGGAATCCAT CAGTTTCAGA ACACTTACCT 
GATGAGAAAG TACAACTGTT TAGCAAAATG AGAGTATTAC AGGAAAAGAA ACAAAAAATG 
GACAAACTAC TTGGAGAACT TCATACACTT CGAGATCAAC ATCTGAACAA TTCATCATTT 
GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT GTGGATCAGA GAAGTACAAC TTCAGCACCC 
TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC AATGGAGAAT CCAACAGCTT CACATCATCT 
GTTCCTTATC CTGTTGCTTC TCTAGTTTCT CAGAATGAGA GTGAAAACGA AGGACATCTA 
AATCCAACTG AAAAACTCCA GAAGTTAAAT GAAGTTCGAA AGAGATTGAA TGAACTAAGA 
GAACTAGTTC ATTATTACGA ACAAACATCA GATATGATGA CAGATGCTGT AAATGAAAAC 
ACAAAAGATG AAGAAACTGA AGAGTCAGAA TATGATTCTG AACATGAAAA CCCTGAACCT 
GTTACTAACA TTCGAAATCC TCAAGTAGCT GCCACTTGGA ATGAAGTAAA TAGTAATTCT 
AATGCACAGT GTGTTTCTAA TAATAGAGAG GGGAGATCAG TTAATTCTAA TTGTGAAATT 
AACAACAGAT CTGCTGCCAA CATAAGGACT CTGAACATGC CTCCTTCTTT AGCAGATTGT 
CACTATAATA GAGAAGGAGA ACAGGGGATT CATGGTGCAC AAGGTGAAGA CGATGAGGAA 
GAAGAAGAAG CAGAAGATGA AGGAGTCAGT GGGGCTTCAT TAACTAGTCA TAGGAGCAGT 
CTGGTTGATG AGGCTGCAGA AGACGCTGAA TTTGAACAGA AGATCAATAG ACTTATGGCT 
GCCAAACAGA AATTAAGACA GTTACAAGAT CTTGTTGCTA TGGTACAGGA TGATGATGCA 
GCAGATCATG GAGTTATCTC TGCCAATACT TCAAATTTGG ATGATTTTTA CCCAGCAGAA 
GAAGACAACA AACAAAGTGC AAATAATACT AGAGGAAATG CCAATAAAAC ACAGAAAGAT 
GCTGGAATAA ATGAGAAGGC AAGAGAGAAG TTTTATGAGG CTAAACTACA GCAGCAACAG 
AGAGAGCTCA GACAATTGCA GGAAGAAAGA AAGAAACTGA TTGAGATTCA AGAGAAAATT 
CAAGCATTAC AGAAGGCATG CCCTGACTTA CAGCTGTCAG CCACTAGTGC GGGTAATTGT 
CCCACAAAAA AATATATCCC AGCTGTTACT TCAACCCCAG TTGTTAATGG GAATGAAACC 
AGTACAAGCA AATCTGCTTT TGAGCCTGCA GATCCTTCAG GAGTGGATAA TGAGCTGTGG 
TCAGAAATGC GAAGACATGA GATGTTAAGG GAAGAGCTAC GGCAGAGAAG AAAGCAGCTT 
GAAGCACTGA TGGCTGAACA TCAGAGGAGG CAAGGTCTAG CTGAAACTAC ATCCCCTCTG 
GCTGTGTCAT TGAGAAGTGA TGGATCTGAG AACCTGTGTA CGCCTCAACA GAGTAGAACA 
GAAAAAACCA TGGCAACATG GGGAGGTTCT ACCCAGTGTG CACTAGATGA AGAAGGAGAT 
GAAGATGGTT ACCTTTCTGA AGGAGTTGTT CGGACAGATG AAGAGGAGGA AGAAGAAGAG 
CAAGATGCAA GTTCGAATGA TAACTTTTCT ATGTATCCTC CTAACAGTGC GAATCATAAC 
TCTTACAACA TAAAAGAAAC TAAGAATAGG TGGAAGAATA GTCGCCCTTT TACAGCAGAT 
GGAAATTATC GTCCTTTAGC CAAGACAAGA CAGCAGAATA TCAGCATGCA ACGTCAGGAA 
AACCTTCGTT GGATGTCCGA ACTCTCTTAC GTAGAGGAGA AAGAACAATG GCAAGAACAA 
ATTAATCAGC TGAAGAAACA ACTTGATTTC AGTGTCAATA TTTGTCAGAC TTTGATGCAA 
GACCAGCAGA CGCTGTCTTG TCTGCTGCAA ACTCTTCTCA CAGGTCCTTA CAGTGTTATG 
CCCAGCAATG TAGCTTCTCC TCAAGTACAC CTTATAATGC ACCAGTTGAA TCAGTGCTAT 
ACTCAGCTAA CATGGCAACA GAATAATGTG CAAAGGTTGA AACAAATGCT AAATGAACTT 
ATGCGCCAAC AGAATCAGCA CCCAGAAAAA CCTGGAAGCC AGGAAAGAGG CAGTAGTGCA 
CCACAGCCTT CTTCTCCCAG TTTATTTTGT CCTTTCAGCT TTCCATCACA GCCTGTAAAT 
CTATTTAACC TACCCGGGTT TACTAATTTT TCATCATTTG CACCAGGTAT GAATTTTAGC 
CCTTTATTTC CTTCTAATTT TGGAGAATTT TCTCAGAATA TTTCTACACC TACTGAACAG 
CAGCAACCAT TAGCCCAGAA TTCTTCAGGG AAAACAGAAT ATATGGCTTT TCCAAAACCT 
TTTGAAAGCA GTTCTTCTAT TGGAGCAGAA AAACAAAGGA ATCAAAAACA GCCTGGAGAG 
GAGGTGGAAA ACAGTAGGAC AGCGTGGTTA TATGACCAAG AAGGTGAAGT AGAAAAACCA 
TTTATCAAGA CTGGATTTCC AGTGTCTGTA GAGAAAACTA CAAATAGTAA TCGCAAAAAT 
CAATTAGATA CCGGCCGAAG AAGACGCCAG TTTGATGAAG AGTCATTAGA AAGCTTTAGC 
AGTATGCCTG ATCCAGTAGA TCCGACAACA GTAACCAAAA CATTCAAGAC GAGAAAAGCA 
TCTGCACAGG CCAGCCTAGC ATCTAAAGAT AAAACTCCTA AATCAAAGAG TAAGAAAAGG 
CATTCTGCTC AACTAAAAAG CAGAGTTAAA AATACTGGGT ATGAAAGTGC CAGTGTGTCT 
AGCACATGTG AACCTTGCAA AAGTAGGAAC AGACATTCAG CCCAGACTGA AGAGCCTGTT 
CAAGCAAAAG TATTCAGCAG AAAGAATCTT GAGCAGCTGG AAAAAATAAT AAAATATAGT 
AGGTCTACAG AGATATCTTC AGCACATGCT AGGAGAATAC TACAGCAGTC TAACAGAAAT 
GCATGCAATG AAGCACCAGA AACTGGGAGT GATTTTTCCA TGTTTGAAGC TTTGCGGGAT 
ACTATTTATT CTGAAGTAGC TACATTAATT TCTCAAAATG AATCTCATCC ACATTTTCTT 
ATTGAACTCT TCCATGAACT TCAGCTACTT AATACAGACT ACTTGAGACA AAGGGCTTTA 
TATGCATTGC AGGACATAGT AACCAGACAT ATTTCTGAGA ACCATGAAAA AGAAGGAGAA 
AATGTAAAGT CAGTGAATTC TGGTACTTGG ATAGCATCAA ACTCAGAGCT TACCCCCAGT 
GAAAGCCTTG CTACCACTGA TGATGAAACT TTTGAGAAGA ACTTTGAGAG AGAAACACAT 
AAAATAAGTG AGCAAAATGA TGCTGATAAT GCTAGCGTCA TGTCTGTATC TTCAAATTTT 
GAGCCTTTTG CAACAGATGA TCTAGGTAAC ACTGTGATTC ACTTAGATCA GGCATTAGCC 
AGAATGAGGG AATACGAGCG TATGAAGACT GAGGCTGAAA GTAGCACAAA TATAAGATGC 
ACCTGCAGAA TTCTTGAGGA TGAGGATGGT GCTGCCGCCA CAAGTATGGT TACTAATTTA 
GAAGAAACTC CTATTGAAAA TCATGGTTCA CAACAACCTG TAAGTGAAGT TTCTACTGTT 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCCAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CTTCTCAACT TCTAACTTCA 
GTAAGGCGTA TGGTTTTGAC CCTCACCCAA CAAAATGATG AGAGCAAAGA ATTTGTGAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTTAATGAA 
TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAAG 
TGCAAAAGGA AAATAGAAGC AGCTGGAGTG ATACAGTCTT ATGCCAAAGA GGCCAAAAGG 
ATTCTTGAAG GAGATCATGG CTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCCGACTCAG ACATCTGAGA TATATGATGG TGATGGTCCC 
AAAAATGTGA GATCAGATGT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGAATGTCCG 
GTGTCTATTA ACTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTATGG AAGTGGAGAA 
GATGAGAATG AGGATGAAGA AATAGAAGAA TTTGAAGAGG GACCTGTGGA TGTCCAGACG 
TCACTTCAGG CTAACACTGA AGCTACAGAA GAAACTGAAC ACGATGATCA GGTACTACAA 
CATGACTTTG AAAAATCAGG AGAAAGCAAA AATGTCCCAT CAGAACAAGA TCCCACTACT 
AGTAAAGGCA AGAAATATGA CCAAGATAGT ACTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAAATG AGCAGCCTTT GAATAGCGCT GTCCAAAAAG ACTCTCTGAC TACCATCGAT 
TCATCTAAAC AGCCTAACCC TTTGCCTTTA CCTTTACCTG AAATCGAAAC CTTGGTGCCT 
ACAGTCAAAG AAGTTAAATC TGCTCAAGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA CATAAGTCAA 
AAATCTGATG AAGAAGACTT TGTGAAAGTT GAAGATTTAC CACTTAAACT GACAATATAC 
TCAGAGGCAG ATCTAAGGAA GAAAATGGTA GAAGAGCAAG AGAAAAACCA TTTATCTGGT 
GAAATACTAT GTGAAATGCA GACCGAAGAA TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA 
CCTGAAACAG TGGGAGCCCA AAGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630013.1
CDS	616..6885
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_005629956.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629956.2

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GAAAAGGTTG CAGGGACAAC ACCTGGCCAT CATACTGTAC CGGGCAGCCA GCCAGCTCGC 
TCTCCTTTTC ATCAGAGAGT ACCCTTAAGA GTTGTAACAG AAACTACAGG TAGCGTATCT 
GGAGTTAGTA TCACCTCTGA ACTAAATGAA GAATTGAATG ATTTAATTCA GCGTTTTCAT 
AATCAGCTTC GTGATTCTCA GCCTCCAACT GTTCCAGACA ATAGAAGACA AGCAGAAAGT 
CTTTCATTAA CTAGGGAGGT ATCCCAGAGC AGGAATCCAT CAGTTTCAGA ACACTTACCT 
GATGAGAAAG TACAACTGTT TAGCAAAATG AGAGTATTAC AGGAAAAGAA ACAAAAAATG 
GACAAACTAC TTGGAGAACT TCATACACTT CGAGATCAAC ATCTGAACAA TTCATCATTT 
GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT GTGGATCAGA GAAGTACAAC TTCAGCACCC 
TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC AATGGAGAAT CCAACAGCTT CACATCATCT 
GTTCCTTATC CTGTTGCTTC TCTAGTTTCT CAGAATGAGA GTGAAAACGA AGGACATCTA 
AATCCAACTG AAAAACTCCA GAAGTTAAAT GAAGTTCGAA AGAGATTGAA TGAACTAAGA 
GAACTAGTTC ATTATTACGA ACAAACATCA GATATGATGA CAGATGCTGT AAATGAAAAC 
ACAAAAGATG AAGAAACTGA AGAGTCAGAA TATGATTCTG AACATGAAAA CCCTGAACCT 
GTTACTAACA TTCGAAATCC TCAAGTAGCT GCCACTTGGA ATGAAGTAAA TAGTAATTCT 
AATGCACAGT GTGTTTCTAA TAATAGAGAG GGGAGATCAG TTAATTCTAA TTGTGAAATT 
AACAACAGAT CTGCTGCCAA CATAAGGACT CTGAACATGC CTCCTTCTTT AGCAGATTGT 
CACTATAATA GAGAAGGAGA ACAGGGGATT CATGGTGCAC AAGGTGAAGA CGATGAGGAA 
GAAGAAGAAG CAGAAGATGA AGGAGTCAGT GGGGCTTCAT TAACTAGTCA TAGGAGCAGT 
CTGGTTGATG AGGCTGCAGA AGACGCTGAA TTTGAACAGA AGATCAATAG ACTTATGGCT 
GCCAAACAGA AATTAAGACA GTTACAAGAT CTTGTTGCTA TGGTACAGGA TGATGATGCA 
GCAGATCATG GAGTTATCTC TGCCAATACT TCAAATTTGG ATGATTTTTA CCCAGCAGAA 
GAAGACAACA AACAAAGTGC AAATAATACT AGAGGAAATG CCAATAAAAC ACAGAAAGAT 
GCTGGAATAA ATGAGAAGGC AAGAGAGAAG TTTTATGAGG CTAAACTACA GCAGCAACAG 
AGAGAGCTCA GACAATTGCA GGAAGAAAGA AAGAAACTGA TTGAGATTCA AGAGAAAATT 
CAAGCATTAC AGAAGGCATG CCCTGACTTA CAGCTGTCAG CCACTAGTGC GGGTAATTGT 
CCCACAAAAA AATATATCCC AGCTGTTACT TCAACCCCAG TTGTTAATGG GAATGAAACC 
AGTACAAGCA AATCTGCTTT TGAGCCTGCA GATCCTTCAG GAGTGGATAA TGAGCTGTGG 
TCAGAAATGC GAAGACATGA GATGTTAAGG GAAGAGCTAC GGCAGAGAAG AAAGCAGCTT 
GAAGCACTGA TGGCTGAACA TCAGAGGAGG CAAGGTCTAG CTGAAACTAC ATCCCCTCTG 
GCTGTGTCAT TGAGAAGTGA TGGATCTGAG AACCTGTGTA CGCCTCAACA GAGTAGAACA 
GAAAAAACCA TGGCAACATG GGGAGGTTCT ACCCAGTGTG CACTAGATGA AGAAGGAGAT 
GAAGATGGTT ACCTTTCTGA AGGAGTTGTT CGGACAGATG AAGAGGAGGA AGAAGAAGAG 
CAAGATGCAA GTTCGAATGA TAACTTTTCT ATGTATCCTC CTAACAGTGC GAATCATAAC 
TCTTACAACA TAAAAGAAAC TAAGAATAGG TGGAAGAATA GTCGCCCTTT TACAGCAGAT 
GGAAATTATC GTCCTTTAGC CAAGACAAGA CAGCAGAATA TCAGCATGCA ACGTCAGGAA 
AACCTTCGTT GGATGTCCGA ACTCTCTTAC GTAGAGGAGA AAGAACAATG GCAAGAACAA 
ATTAATCAGC TGAAGAAACA ACTTGATTTC AGTGTCAATA TTTGTCAGAC TTTGATGCAA 
GACCAGCAGA CGCTGTCTTG TCTGCTGCAA ACTCTTCTCA CAGGTCCTTA CAGTGTTATG 
CCCAGCAATG TAGCTTCTCC TCAAGTACAC CTTATAATGC ACCAGTTGAA TCAGTGCTAT 
ACTCAGCTAA CATGGCAACA GAATAATGTG CAAAGGTTGA AACAAATGCT AAATGAACTT 
ATGCGCCAAC AGAATCAGCA CCCAGAAAAA CCTGGAAGCC AGGAAAGAGG CAGTAGTGCA 
CCACAGCCTT CTTCTCCCAG TTTATTTTGT CCTTTCAGCT TTCCATCACA GCCTGTAAAT 
CTATTTAACC TACCCGGGTT TACTAATTTT TCATCATTTG CACCAGGTAT GAATTTTAGC 
CCTTTATTTC CTTCTAATTT TGGAGAATTT TCTCAGAATA TTTCTACACC TACTGAACAG 
CAGCAACCAT TAGCCCAGAA TTCTTCAGGG AAAACAGAAT ATATGGCTTT TCCAAAACCT 
TTTGAAAGCA GTTCTTCTAT TGGAGCAGAA AAACAAAGGA ATCAAAAACA GCCTGGAGAG 
GAGGTGGAAA ACAGTAGGAC AGCGTGGTTA TATGACCAAG AAGGTGAAGT AGAAAAACCA 
TTTATCAAGA CTGGATTTCC AGTGTCTGTA GAGAAAACTA CAAATAGTAA TCGCAAAAAT 
CAATTAGATA CCGGCCGAAG AAGACGCCAG TTTGATGAAG AGTCATTAGA AAGCTTTAGC 
AGTATGCCTG ATCCAGTAGA TCCGACAACA GTAACCAAAA CATTCAAGAC GAGAAAAGCA 
TCTGCACAGG CCAGCCTAGC ATCTAAAGAT AAAACTCCTA AATCAAAGAG TAAGAAAAGG 
CATTCTGCTC AACTAAAAAG CAGAGTTAAA AATACTGGGT ATGAAAGTGC CAGTGTGTCT 
AGCACATGTG AACCTTGCAA AAGTAGGAAC AGACATTCAG CCCAGACTGA AGAGCCTGTT 
CAAGCAAAAG TATTCAGCAG AAAGAATCTT GAGCAGCTGG AAAAAATAAT AAAATATAGT 
AGGTCTACAG AGATATCTTC AGCACATGCT AGGAGAATAC TACAGCAGTC TAACAGAAAT 
GCATGCAATG AAGCACCAGA AACTGGGAGT GATTTTTCCA TGTTTGAAGC TTTGCGGGAT 
ACTATTTATT CTGAAGTAGC TACATTAATT TCTCAAAATG AATCTCATCC ACATTTTCTT 
ATTGAACTCT TCCATGAACT TCAGCTACTT AATACAGACT ACTTGAGACA AAGGGCTTTA 
TATGCATTGC AGGACATAGT AACCAGACAT ATTTCTGAGA ACCATGAAAA AGAAGGAGAA 
AATGTAAAGT CAGTGAATTC TGGTACTTGG ATAGCATCAA ACTCAGAGCT TACCCCCAGT 
GAAAGCCTTG CTACCACTGA TGATGAAACT TTTGAGAAGA ACTTTGAGAG AGAAACACAT 
AAAATAAGTG AGCAAAATGA TGCTGATAAT GCTAGCGTCA TGTCTGTATC TTCAAATTTT 
GAGCCTTTTG CAACAGATGA TCTAGGTAAC ACTGTGATTC ACTTAGATCA GGCATTAGCC 
AGAATGAGGG AATACGAGCG TATGAAGACT GAGGCTGAAA GTAGCACAAA TATAAGATGC 
ACCTGCAGAA TTCTTGAGGA TGAGGATGGT GCTGCCGCCA CAAGTATGGT TACTAATTTA 
GAAGAAACTC CTATTGAAAA TCATGGTTCA CAACAACCTG TAAGTGAAGT TTCTACTGTT 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCCAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CTTCTCAACT TCTAACTTCA 
GTAAGGCGTA TGGTTTTGAC CCTCACCCAA CAAAATGATG AGAGCAAAGA ATTTGTGAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTTAATGAA 
TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAAG 
TGCAAAAGGA AAATAGAAGC AGCTGGAGTG ATACAGTCTT ATGCCAAAGA GGCCAAAAGG 
ATTCTTGAAG GAGATCATGG CTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCCGACTCAG ACATCTGAGA TATATGATGG TGATGGTCCC 
AAAAATGTGA GATCAGATGT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGAATGTCCG 
GTGTCTATTA ACTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTATGG AAGTGGAGAA 
GATGAGAATG AGGATGAAGA AATAGAAGAA TTTGAAGAGG GACCTGTGGA TGTCCAGACG 
TCACTTCAGG CTAACACTGA AGCTACAGAA GAAACTGAAC ACGATGATCA GGTACTACAA 
CATGACTTTG AAAAATCAGG AGAAAGCAAA AATGTCCCAT CAGAACAAGA TCCCACTACT 
AGTAAAGGCA AGAAATATGA CCAAGATAGT ACTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAAATG AGCAGCCTTT GAATAGCGCT GTCCAAAAAG ACTCTCTGAC TACCATCGAT 
TCATCTAAAC AGCCTAACCC TTTGCCTTTA CCTTTACCTG AAATCGAAAC CTTGGTGCCT 
ACAGTCAAAG AAGTTAAATC TGCTCAAGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA CATAAGTCAA 
AAATCTGATG AAGAAGACTT TGTGAAAGTT GAAGATTTAC CACTTAAACT GACAATATAC 
TCAGAGGCAG ATCTAAGGAA GAAAATGGTA GAAGAGCAAG AGAAAAACCA TTTATCTGGT 
GAAATACTAT GTGAAATGCA GACCGAAGAA TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA 
CCTGAAACAG TGGGAGCCCA AAGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_022404155.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259863.1 Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X16, mRNA.

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CloneID	OCe18442
Clone ID Related Accession (Same CDS sequence)	XM_005629956.2 , XM_022404155.1
Accession Version	XM_022404155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pericentriolar material 1 protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GAAAAGGTTG CAGGGACAAC ACCTGGCCAT CATACTGTAC CGGGCAGCCA GCCAGCTCGC 
TCTCCTTTTC ATCAGAGAGT ACCCTTAAGA GTTGTAACAG AAACTACAGG TAGCGTATCT 
GGAGTTAGTA TCACCTCTGA ACTAAATGAA GAATTGAATG ATTTAATTCA GCGTTTTCAT 
AATCAGCTTC GTGATTCTCA GCCTCCAACT GTTCCAGACA ATAGAAGACA AGCAGAAAGT 
CTTTCATTAA CTAGGGAGGT ATCCCAGAGC AGGAATCCAT CAGTTTCAGA ACACTTACCT 
GATGAGAAAG TACAACTGTT TAGCAAAATG AGAGTATTAC AGGAAAAGAA ACAAAAAATG 
GACAAACTAC TTGGAGAACT TCATACACTT CGAGATCAAC ATCTGAACAA TTCATCATTT 
GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT GTGGATCAGA GAAGTACAAC TTCAGCACCC 
TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC AATGGAGAAT CCAACAGCTT CACATCATCT 
GTTCCTTATC CTGTTGCTTC TCTAGTTTCT CAGAATGAGA GTGAAAACGA AGGACATCTA 
AATCCAACTG AAAAACTCCA GAAGTTAAAT GAAGTTCGAA AGAGATTGAA TGAACTAAGA 
GAACTAGTTC ATTATTACGA ACAAACATCA GATATGATGA CAGATGCTGT AAATGAAAAC 
ACAAAAGATG AAGAAACTGA AGAGTCAGAA TATGATTCTG AACATGAAAA CCCTGAACCT 
GTTACTAACA TTCGAAATCC TCAAGTAGCT GCCACTTGGA ATGAAGTAAA TAGTAATTCT 
AATGCACAGT GTGTTTCTAA TAATAGAGAG GGGAGATCAG TTAATTCTAA TTGTGAAATT 
AACAACAGAT CTGCTGCCAA CATAAGGACT CTGAACATGC CTCCTTCTTT AGCAGATTGT 
CACTATAATA GAGAAGGAGA ACAGGGGATT CATGGTGCAC AAGGTGAAGA CGATGAGGAA 
GAAGAAGAAG CAGAAGATGA AGGAGTCAGT GGGGCTTCAT TAACTAGTCA TAGGAGCAGT 
CTGGTTGATG AGGCTGCAGA AGACGCTGAA TTTGAACAGA AGATCAATAG ACTTATGGCT 
GCCAAACAGA AATTAAGACA GTTACAAGAT CTTGTTGCTA TGGTACAGGA TGATGATGCA 
GCAGATCATG GAGTTATCTC TGCCAATACT TCAAATTTGG ATGATTTTTA CCCAGCAGAA 
GAAGACAACA AACAAAGTGC AAATAATACT AGAGGAAATG CCAATAAAAC ACAGAAAGAT 
GCTGGAATAA ATGAGAAGGC AAGAGAGAAG TTTTATGAGG CTAAACTACA GCAGCAACAG 
AGAGAGCTCA GACAATTGCA GGAAGAAAGA AAGAAACTGA TTGAGATTCA AGAGAAAATT 
CAAGCATTAC AGAAGGCATG CCCTGACTTA CAGCTGTCAG CCACTAGTGC GGGTAATTGT 
CCCACAAAAA AATATATCCC AGCTGTTACT TCAACCCCAG TTGTTAATGG GAATGAAACC 
AGTACAAGCA AATCTGCTTT TGAGCCTGCA GATCCTTCAG GAGTGGATAA TGAGCTGTGG 
TCAGAAATGC GAAGACATGA GATGTTAAGG GAAGAGCTAC GGCAGAGAAG AAAGCAGCTT 
GAAGCACTGA TGGCTGAACA TCAGAGGAGG CAAGGTCTAG CTGAAACTAC ATCCCCTCTG 
GCTGTGTCAT TGAGAAGTGA TGGATCTGAG AACCTGTGTA CGCCTCAACA GAGTAGAACA 
GAAAAAACCA TGGCAACATG GGGAGGTTCT ACCCAGTGTG CACTAGATGA AGAAGGAGAT 
GAAGATGGTT ACCTTTCTGA AGGAGTTGTT CGGACAGATG AAGAGGAGGA AGAAGAAGAG 
CAAGATGCAA GTTCGAATGA TAACTTTTCT ATGTATCCTC CTAACAGTGC GAATCATAAC 
TCTTACAACA TAAAAGAAAC TAAGAATAGG TGGAAGAATA GTCGCCCTTT TACAGCAGAT 
GGAAATTATC GTCCTTTAGC CAAGACAAGA CAGCAGAATA TCAGCATGCA ACGTCAGGAA 
AACCTTCGTT GGATGTCCGA ACTCTCTTAC GTAGAGGAGA AAGAACAATG GCAAGAACAA 
ATTAATCAGC TGAAGAAACA ACTTGATTTC AGTGTCAATA TTTGTCAGAC TTTGATGCAA 
GACCAGCAGA CGCTGTCTTG TCTGCTGCAA ACTCTTCTCA CAGGTCCTTA CAGTGTTATG 
CCCAGCAATG TAGCTTCTCC TCAAGTACAC CTTATAATGC ACCAGTTGAA TCAGTGCTAT 
ACTCAGCTAA CATGGCAACA GAATAATGTG CAAAGGTTGA AACAAATGCT AAATGAACTT 
ATGCGCCAAC AGAATCAGCA CCCAGAAAAA CCTGGAAGCC AGGAAAGAGG CAGTAGTGCA 
CCACAGCCTT CTTCTCCCAG TTTATTTTGT CCTTTCAGCT TTCCATCACA GCCTGTAAAT 
CTATTTAACC TACCCGGGTT TACTAATTTT TCATCATTTG CACCAGGTAT GAATTTTAGC 
CCTTTATTTC CTTCTAATTT TGGAGAATTT TCTCAGAATA TTTCTACACC TACTGAACAG 
CAGCAACCAT TAGCCCAGAA TTCTTCAGGG AAAACAGAAT ATATGGCTTT TCCAAAACCT 
TTTGAAAGCA GTTCTTCTAT TGGAGCAGAA AAACAAAGGA ATCAAAAACA GCCTGGAGAG 
GAGGTGGAAA ACAGTAGGAC AGCGTGGTTA TATGACCAAG AAGGTGAAGT AGAAAAACCA 
TTTATCAAGA CTGGATTTCC AGTGTCTGTA GAGAAAACTA CAAATAGTAA TCGCAAAAAT 
CAATTAGATA CCGGCCGAAG AAGACGCCAG TTTGATGAAG AGTCATTAGA AAGCTTTAGC 
AGTATGCCTG ATCCAGTAGA TCCGACAACA GTAACCAAAA CATTCAAGAC GAGAAAAGCA 
TCTGCACAGG CCAGCCTAGC ATCTAAAGAT AAAACTCCTA AATCAAAGAG TAAGAAAAGG 
CATTCTGCTC AACTAAAAAG CAGAGTTAAA AATACTGGGT ATGAAAGTGC CAGTGTGTCT 
AGCACATGTG AACCTTGCAA AAGTAGGAAC AGACATTCAG CCCAGACTGA AGAGCCTGTT 
CAAGCAAAAG TATTCAGCAG AAAGAATCTT GAGCAGCTGG AAAAAATAAT AAAATATAGT 
AGGTCTACAG AGATATCTTC AGCACATGCT AGGAGAATAC TACAGCAGTC TAACAGAAAT 
GCATGCAATG AAGCACCAGA AACTGGGAGT GATTTTTCCA TGTTTGAAGC TTTGCGGGAT 
ACTATTTATT CTGAAGTAGC TACATTAATT TCTCAAAATG AATCTCATCC ACATTTTCTT 
ATTGAACTCT TCCATGAACT TCAGCTACTT AATACAGACT ACTTGAGACA AAGGGCTTTA 
TATGCATTGC AGGACATAGT AACCAGACAT ATTTCTGAGA ACCATGAAAA AGAAGGAGAA 
AATGTAAAGT CAGTGAATTC TGGTACTTGG ATAGCATCAA ACTCAGAGCT TACCCCCAGT 
GAAAGCCTTG CTACCACTGA TGATGAAACT TTTGAGAAGA ACTTTGAGAG AGAAACACAT 
AAAATAAGTG AGCAAAATGA TGCTGATAAT GCTAGCGTCA TGTCTGTATC TTCAAATTTT 
GAGCCTTTTG CAACAGATGA TCTAGGTAAC ACTGTGATTC ACTTAGATCA GGCATTAGCC 
AGAATGAGGG AATACGAGCG TATGAAGACT GAGGCTGAAA GTAGCACAAA TATAAGATGC 
ACCTGCAGAA TTCTTGAGGA TGAGGATGGT GCTGCCGCCA CAAGTATGGT TACTAATTTA 
GAAGAAACTC CTATTGAAAA TCATGGTTCA CAACAACCTG TAAGTGAAGT TTCTACTGTT 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCCAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CTTCTCAACT TCTAACTTCA 
GTAAGGCGTA TGGTTTTGAC CCTCACCCAA CAAAATGATG AGAGCAAAGA ATTTGTGAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTTAATGAA 
TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAAG 
TGCAAAAGGA AAATAGAAGC AGCTGGAGTG ATACAGTCTT ATGCCAAAGA GGCCAAAAGG 
ATTCTTGAAG GAGATCATGG CTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCCGACTCAG ACATCTGAGA TATATGATGG TGATGGTCCC 
AAAAATGTGA GATCAGATGT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGAATGTCCG 
GTGTCTATTA ACTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTATGG AAGTGGAGAA 
GATGAGAATG AGGATGAAGA AATAGAAGAA TTTGAAGAGG GACCTGTGGA TGTCCAGACG 
TCACTTCAGG CTAACACTGA AGCTACAGAA GAAACTGAAC ACGATGATCA GGTACTACAA 
CATGACTTTG AAAAATCAGG AGAAAGCAAA AATGTCCCAT CAGAACAAGA TCCCACTACT 
AGTAAAGGCA AGAAATATGA CCAAGATAGT ACTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAAATG AGCAGCCTTT GAATAGCGCT GTCCAAAAAG ACTCTCTGAC TACCATCGAT 
TCATCTAAAC AGCCTAACCC TTTGCCTTTA CCTTTACCTG AAATCGAAAC CTTGGTGCCT 
ACAGTCAAAG AAGTTAAATC TGCTCAAGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA CATAAGTCAA 
AAATCTGATG AAGAAGACTT TGTGAAAGTT GAAGATTTAC CACTTAAACT GACAATATAC 
TCAGAGGCAG ATCTAAGGAA GAAAATGGTA GAAGAGCAAG AGAAAAACCA TTTATCTGGT 
GAAATACTAT GTGAAATGCA GACCGAAGAA TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA 
CCTGAAACAG TGGGAGCCCA AAGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259863.1
CDS	426..6695
Translation	MATGGGPFEEGINDQDLPNWSNEGVDDRLNNMDWGGQQKKANKSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHSFPHSRYVTQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDAAISPPKREMIGSTQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLQKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSEKVAGTTPGHHTVPGSQPARSPFHQRVPLRVVTETTGSVSGVSITSELNEELNDLIQRFHNQLRDSQPPTVPDNRRQAESLSLTREVSQSRNPSVSEHLPDEKVQLFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSFVPSSASPQRSVDQRSTTSAPSAPIGLAPVVNGESNSFTSSVPYPVASLVSQNESENEGHLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENTKDEETEESEYDSEHENPEPVTNIRNPQVAATWNEVNSNSNAQCVSNNREGRSVNSNCEINNRSAANIRTLNMPPSLADCHYNREGEQGIHGAQGEDDEEEEEAEDEGVSGASLTSHRSSLVDEAAEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAADHGVISANTSNLDDFYPAEEDNKQSANNTRGNANKTQKDAGINEKAREKFYEAKLQQQQRELRQLQEERKKLIEIQEKIQALQKACPDLQLSATSAGNCPTKKYIPAVTSTPVVNGNETSTSKSAFEPADPSGVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETTSPLAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGVVRTDEEEEEEEQDASSNDNFSMYPPNSANHNSYNIKETKNRWKNSRPFTADGNYRPLAKTRQQNISMQRQENLRWMSELSYVEEKEQWQEQINQLKKQLDFSVNICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGSQERGSSAPQPSSPSLFCPFSFPSQPVNLFNLPGFTNFSSFAPGMNFSPLFPSNFGEFSQNISTPTEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKQRNQKQPGEEVENSRTAWLYDQEGEVEKPFIKTGFPVSVEKTTNSNRKNQLDTGRRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRHSAQLKSRVKNTGYESASVSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNLEQLEKIIKYSRSTEISSAHARRILQQSNRNACNEAPETGSDFSMFEALRDTIYSEVATLISQNESHPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHISENHEKEGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVMSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESSTNIRCTCRILEDEDGAAATSMVTNLEETPIENHGSQQPVSEVSTVPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQKCKRKIEAAGVIQSYAKEAKRILEGDHGSPAGEIDDEDKDKDETETVKPTQTSEIYDGDGPKNVRSDVSDQEEDEESEECPVSINLSKAETQALTNYGSGEDENEDEEIEEFEEGPVDVQTSLQANTEATEETEHDDQVLQHDFEKSGESKNVPSEQDPTTSKGKKYDQDSTPVKPCYLNILENEQPLNSAVQKDSLTTIDSSKQPNPLPLPLPEIETLVPTVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEQEKNHLSGEILCEMQTEELAGNSQTLKEPETVGAQSV

Target ORF information:

RefSeq Version	XM_022404155.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pericentriolar material 1 (PCM1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404155.1

ORF Insert Sequence:

ATGGCCACAG GAGGAGGTCC TTTTGAAGAA GGCATAAATG ATCAGGATTT ACCAAACTGG 
AGCAATGAGG GTGTTGATGA CCGACTCAAC AATATGGATT GGGGTGGCCA ACAGAAGAAA 
GCAAATAAAT CATCAGAAAA GAATAAGAAA AAGTTTGGTG TAGAAAGTGA TAAAAGGGTA 
ACTAATGATA TTTCTCCGGA GTCATCACCA GGAGTGGGAA GGCGAAGAAC AAAGACTCCA 
CATTCGTTTC CACATAGTAG ATATGTGACT CAGATGTCTG TCCCAGAGCA GGCCGAACTA 
GAGAAACTTA AACAGCGGAT AAACTTCAGT GATTTAGATC AGAGAAGCAT TGGAAGCGAT 
TCTCAAGGGA GAGCAACAGC TGCTAACAAC AAACGTCAGC TTAGTGAGAA CCGAAAGCCC 
TTCAACTTTT TACCTATGCA GATTAATACT AACAAGAGCA AAGATGCTGC TATAAGTCCT 
CCAAAGAGAG AAATGATTGG GTCAACACAA TGTAAAGAGT TGTTTGCTTC CGCTTTAAGT 
AATGATCTTT TGCAAAACTG TCAAGTCTCT GAAGAAGATG GAAGAGGAGA GCCTGCAATG 
GAGAGCAGCC AGATTGTAAG CAGGCTCGTT CAGATTCGTG ATTATATTAC TAAAGCTAGT 
TCCATGCGGG AAGATCTTGT AGAGAAAAAT GAAAGATCTG CTAATGTTGA GCGCCTTACT 
CATCTAATAG ATCACCTTAA AGAACAAGAA AAGTCATATA TGAAATTTCT TCAAAAAATC 
CTTGCCAGAG ATCCTCAGCA GGAGCCTATG GAAGAGATAG AAAATTTGAA GAAACAGCAT 
GACTTATTAA AAAGGATGTT ACAACAGCAG GAGCAACTGA GAGCTCTGCA GGGAAGACAG 
GCTGCTCTTC TTGCTCTGCA GCACAAAGCA GAGCAAGCTA TTGCTGTGAT GGATGATTCT 
GAAAAGGTTG CAGGGACAAC ACCTGGCCAT CATACTGTAC CGGGCAGCCA GCCAGCTCGC 
TCTCCTTTTC ATCAGAGAGT ACCCTTAAGA GTTGTAACAG AAACTACAGG TAGCGTATCT 
GGAGTTAGTA TCACCTCTGA ACTAAATGAA GAATTGAATG ATTTAATTCA GCGTTTTCAT 
AATCAGCTTC GTGATTCTCA GCCTCCAACT GTTCCAGACA ATAGAAGACA AGCAGAAAGT 
CTTTCATTAA CTAGGGAGGT ATCCCAGAGC AGGAATCCAT CAGTTTCAGA ACACTTACCT 
GATGAGAAAG TACAACTGTT TAGCAAAATG AGAGTATTAC AGGAAAAGAA ACAAAAAATG 
GACAAACTAC TTGGAGAACT TCATACACTT CGAGATCAAC ATCTGAACAA TTCATCATTT 
GTGCCTTCTT CAGCTTCTCC ACAAAGAAGT GTGGATCAGA GAAGTACAAC TTCAGCACCC 
TCTGCTCCTA TAGGCTTAGC ACCAGTTGTC AATGGAGAAT CCAACAGCTT CACATCATCT 
GTTCCTTATC CTGTTGCTTC TCTAGTTTCT CAGAATGAGA GTGAAAACGA AGGACATCTA 
AATCCAACTG AAAAACTCCA GAAGTTAAAT GAAGTTCGAA AGAGATTGAA TGAACTAAGA 
GAACTAGTTC ATTATTACGA ACAAACATCA GATATGATGA CAGATGCTGT AAATGAAAAC 
ACAAAAGATG AAGAAACTGA AGAGTCAGAA TATGATTCTG AACATGAAAA CCCTGAACCT 
GTTACTAACA TTCGAAATCC TCAAGTAGCT GCCACTTGGA ATGAAGTAAA TAGTAATTCT 
AATGCACAGT GTGTTTCTAA TAATAGAGAG GGGAGATCAG TTAATTCTAA TTGTGAAATT 
AACAACAGAT CTGCTGCCAA CATAAGGACT CTGAACATGC CTCCTTCTTT AGCAGATTGT 
CACTATAATA GAGAAGGAGA ACAGGGGATT CATGGTGCAC AAGGTGAAGA CGATGAGGAA 
GAAGAAGAAG CAGAAGATGA AGGAGTCAGT GGGGCTTCAT TAACTAGTCA TAGGAGCAGT 
CTGGTTGATG AGGCTGCAGA AGACGCTGAA TTTGAACAGA AGATCAATAG ACTTATGGCT 
GCCAAACAGA AATTAAGACA GTTACAAGAT CTTGTTGCTA TGGTACAGGA TGATGATGCA 
GCAGATCATG GAGTTATCTC TGCCAATACT TCAAATTTGG ATGATTTTTA CCCAGCAGAA 
GAAGACAACA AACAAAGTGC AAATAATACT AGAGGAAATG CCAATAAAAC ACAGAAAGAT 
GCTGGAATAA ATGAGAAGGC AAGAGAGAAG TTTTATGAGG CTAAACTACA GCAGCAACAG 
AGAGAGCTCA GACAATTGCA GGAAGAAAGA AAGAAACTGA TTGAGATTCA AGAGAAAATT 
CAAGCATTAC AGAAGGCATG CCCTGACTTA CAGCTGTCAG CCACTAGTGC GGGTAATTGT 
CCCACAAAAA AATATATCCC AGCTGTTACT TCAACCCCAG TTGTTAATGG GAATGAAACC 
AGTACAAGCA AATCTGCTTT TGAGCCTGCA GATCCTTCAG GAGTGGATAA TGAGCTGTGG 
TCAGAAATGC GAAGACATGA GATGTTAAGG GAAGAGCTAC GGCAGAGAAG AAAGCAGCTT 
GAAGCACTGA TGGCTGAACA TCAGAGGAGG CAAGGTCTAG CTGAAACTAC ATCCCCTCTG 
GCTGTGTCAT TGAGAAGTGA TGGATCTGAG AACCTGTGTA CGCCTCAACA GAGTAGAACA 
GAAAAAACCA TGGCAACATG GGGAGGTTCT ACCCAGTGTG CACTAGATGA AGAAGGAGAT 
GAAGATGGTT ACCTTTCTGA AGGAGTTGTT CGGACAGATG AAGAGGAGGA AGAAGAAGAG 
CAAGATGCAA GTTCGAATGA TAACTTTTCT ATGTATCCTC CTAACAGTGC GAATCATAAC 
TCTTACAACA TAAAAGAAAC TAAGAATAGG TGGAAGAATA GTCGCCCTTT TACAGCAGAT 
GGAAATTATC GTCCTTTAGC CAAGACAAGA CAGCAGAATA TCAGCATGCA ACGTCAGGAA 
AACCTTCGTT GGATGTCCGA ACTCTCTTAC GTAGAGGAGA AAGAACAATG GCAAGAACAA 
ATTAATCAGC TGAAGAAACA ACTTGATTTC AGTGTCAATA TTTGTCAGAC TTTGATGCAA 
GACCAGCAGA CGCTGTCTTG TCTGCTGCAA ACTCTTCTCA CAGGTCCTTA CAGTGTTATG 
CCCAGCAATG TAGCTTCTCC TCAAGTACAC CTTATAATGC ACCAGTTGAA TCAGTGCTAT 
ACTCAGCTAA CATGGCAACA GAATAATGTG CAAAGGTTGA AACAAATGCT AAATGAACTT 
ATGCGCCAAC AGAATCAGCA CCCAGAAAAA CCTGGAAGCC AGGAAAGAGG CAGTAGTGCA 
CCACAGCCTT CTTCTCCCAG TTTATTTTGT CCTTTCAGCT TTCCATCACA GCCTGTAAAT 
CTATTTAACC TACCCGGGTT TACTAATTTT TCATCATTTG CACCAGGTAT GAATTTTAGC 
CCTTTATTTC CTTCTAATTT TGGAGAATTT TCTCAGAATA TTTCTACACC TACTGAACAG 
CAGCAACCAT TAGCCCAGAA TTCTTCAGGG AAAACAGAAT ATATGGCTTT TCCAAAACCT 
TTTGAAAGCA GTTCTTCTAT TGGAGCAGAA AAACAAAGGA ATCAAAAACA GCCTGGAGAG 
GAGGTGGAAA ACAGTAGGAC AGCGTGGTTA TATGACCAAG AAGGTGAAGT AGAAAAACCA 
TTTATCAAGA CTGGATTTCC AGTGTCTGTA GAGAAAACTA CAAATAGTAA TCGCAAAAAT 
CAATTAGATA CCGGCCGAAG AAGACGCCAG TTTGATGAAG AGTCATTAGA AAGCTTTAGC 
AGTATGCCTG ATCCAGTAGA TCCGACAACA GTAACCAAAA CATTCAAGAC GAGAAAAGCA 
TCTGCACAGG CCAGCCTAGC ATCTAAAGAT AAAACTCCTA AATCAAAGAG TAAGAAAAGG 
CATTCTGCTC AACTAAAAAG CAGAGTTAAA AATACTGGGT ATGAAAGTGC CAGTGTGTCT 
AGCACATGTG AACCTTGCAA AAGTAGGAAC AGACATTCAG CCCAGACTGA AGAGCCTGTT 
CAAGCAAAAG TATTCAGCAG AAAGAATCTT GAGCAGCTGG AAAAAATAAT AAAATATAGT 
AGGTCTACAG AGATATCTTC AGCACATGCT AGGAGAATAC TACAGCAGTC TAACAGAAAT 
GCATGCAATG AAGCACCAGA AACTGGGAGT GATTTTTCCA TGTTTGAAGC TTTGCGGGAT 
ACTATTTATT CTGAAGTAGC TACATTAATT TCTCAAAATG AATCTCATCC ACATTTTCTT 
ATTGAACTCT TCCATGAACT TCAGCTACTT AATACAGACT ACTTGAGACA AAGGGCTTTA 
TATGCATTGC AGGACATAGT AACCAGACAT ATTTCTGAGA ACCATGAAAA AGAAGGAGAA 
AATGTAAAGT CAGTGAATTC TGGTACTTGG ATAGCATCAA ACTCAGAGCT TACCCCCAGT 
GAAAGCCTTG CTACCACTGA TGATGAAACT TTTGAGAAGA ACTTTGAGAG AGAAACACAT 
AAAATAAGTG AGCAAAATGA TGCTGATAAT GCTAGCGTCA TGTCTGTATC TTCAAATTTT 
GAGCCTTTTG CAACAGATGA TCTAGGTAAC ACTGTGATTC ACTTAGATCA GGCATTAGCC 
AGAATGAGGG AATACGAGCG TATGAAGACT GAGGCTGAAA GTAGCACAAA TATAAGATGC 
ACCTGCAGAA TTCTTGAGGA TGAGGATGGT GCTGCCGCCA CAAGTATGGT TACTAATTTA 
GAAGAAACTC CTATTGAAAA TCATGGTTCA CAACAACCTG TAAGTGAAGT TTCTACTGTT 
CCATGTCCTA GAATTGATAC TCAGCAGCTG GACCGGCAAA TTAAAGCCAT TATGAAAGAA 
GTCATTCCTT TTTTGAAGGA GCACATGGAT GAAGTATGCT CTTCTCAACT TCTAACTTCA 
GTAAGGCGTA TGGTTTTGAC CCTCACCCAA CAAAATGATG AGAGCAAAGA ATTTGTGAAG 
TTCTTTCATA AACAACTTGG AAGTATATTA CAGGATTCAC TGGCAAAATT TGCTGGCAGA 
AAACTGAAAG ACTGTGGAGA AGATCTTCTT GTAGAGATAT CTGAAGTGTT GTTTAATGAA 
TTGGCTTTCT TTAAGCTCAT GCAAGATTTG GATAATAATA GTATAACTGT TAAACAGAAG 
TGCAAAAGGA AAATAGAAGC AGCTGGAGTG ATACAGTCTT ATGCCAAAGA GGCCAAAAGG 
ATTCTTGAAG GAGATCATGG CTCACCTGCT GGAGAAATTG ATGATGAAGA CAAAGACAAG 
GATGAAACTG AAACAGTTAA GCCGACTCAG ACATCTGAGA TATATGATGG TGATGGTCCC 
AAAAATGTGA GATCAGATGT TTCTGATCAA GAGGAAGATG AAGAAAGTGA AGAATGTCCG 
GTGTCTATTA ACTTGTCCAA AGCTGAAACT CAGGCTTTGA CTAATTATGG AAGTGGAGAA 
GATGAGAATG AGGATGAAGA AATAGAAGAA TTTGAAGAGG GACCTGTGGA TGTCCAGACG 
TCACTTCAGG CTAACACTGA AGCTACAGAA GAAACTGAAC ACGATGATCA GGTACTACAA 
CATGACTTTG AAAAATCAGG AGAAAGCAAA AATGTCCCAT CAGAACAAGA TCCCACTACT 
AGTAAAGGCA AGAAATATGA CCAAGATAGT ACTCCTGTGA AACCCTGTTA CCTCAATATC 
TTGGAAAATG AGCAGCCTTT GAATAGCGCT GTCCAAAAAG ACTCTCTGAC TACCATCGAT 
TCATCTAAAC AGCCTAACCC TTTGCCTTTA CCTTTACCTG AAATCGAAAC CTTGGTGCCT 
ACAGTCAAAG AAGTTAAATC TGCTCAAGAA ACTCCTGAAA GCTCTCTGGC TGGAAGTCCT 
GATACTGAAT CTCCAGTGTT AGTGAATGAC TATGAAGCAG AATCTGGTAA CATAAGTCAA 
AAATCTGATG AAGAAGACTT TGTGAAAGTT GAAGATTTAC CACTTAAACT GACAATATAC 
TCAGAGGCAG ATCTAAGGAA GAAAATGGTA GAAGAGCAAG AGAAAAACCA TTTATCTGGT 
GAAATACTAT GTGAAATGCA GACCGAAGAA TTAGCTGGGA ATTCTCAGAC ACTAAAAGAA 
CCTGAAACAG TGGGAGCCCA AAGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

PCM1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: