ATP6V0A4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP6V0A4
Entrez Gene ID	50617
Full Name	ATPase H+ transporting V0 subunit a4
Synonyms	A4,ATP6N1B,ATP6N2,RDRTA2,RTA1C,RTADR,STV1,VPH1,VPP2
General protein information	Preferred Names ATPase H+ transporting V0 subunit a4 Names V-type proton ATPase 116 kDa subunit a isoform 4 V-type proton ATPase 116 kDa subunit a ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 1B ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 2 (38kD) ATPase, H+ transporting, lysosomal V0 subunit a4 H(+)-transporting two-sector ATPase, noncatalytic accessory protein 1B V-ATPase 116 kDa vacuolar proton pump 116 kDa accessory subunit vacuolar proton pump, subunit 2 vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q34
Summary	This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq, Jul 2008].
Disorder MIM:	605239
Disorder Html:	Renal tubular acidosis, distal, autosomal recessive, 602722 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005250393.1	XP_005250450.1	V-type proton ATPase 116 kDa subunit a isoform X1
XM_005250394.3	XP_005250451.1	V-type proton ATPase 116 kDa subunit a isoform X1
NM_130841.2	NP_570856.2	V-type proton ATPase 116 kDa subunit a isoform 4
NM_020632.3	NP_065683.2	V-type proton ATPase 116 kDa subunit a isoform 4
NM_020632.2	NP_065683.2	V-type proton ATPase 116 kDa subunit a isoform 4
NM_130840.3	NP_570855.2	V-type proton ATPase 116 kDa subunit a isoform 4
NM_130840.2	NP_570855.2	V-type proton ATPase 116 kDa subunit a isoform 4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP6V0A4	XP_539895.2
Rattus norvegicus (Norway rat)	Atp6v0a4	NP_001100061.1
Pan troglodytes (chimpanzee)	ATP6V0A4	XP_003318871.1
Bos taurus (cattle)	ATP6V0A4	NP_001192780.1
Mus musculus (house mouse)	Atp6v0a4	NP_536715.3
Xenopus tropicalis (tropical clawed frog)	atp6v0a4	NP_001107162.1
Gallus gallus (chicken)	ATP6V0A4	NP_001073571.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001587	XP_321521.2
Arabidopsis thaliana (thale cress)	VHA-A3	NP_568051.1
Arabidopsis thaliana (thale cress)	VHA-A2	NP_179736.1
Homo sapiens (human)	ATP6V0A4	NP_570856.2
	ATP6V0A4	XP_002803521.1
Drosophila melanogaster (fruit fly)	Vha100-2	NP_650722.1
Oryza sativa (rice)	Os03g0251500	NP_001049572.1

Gene Ontology
Bibliography

Related articles in PubMed

Distal renal tubular acidosis in a Libyan patient: Evidence for digenic inheritance.
Nagara M, Papagregoriou G, Ben Abdallah R, Landoulsi Z, Bouyacoub Y, Elouej S, Kefi R, Pippucci T, Voskarides K, Bashamboo A, McElreavey K, Hachicha M, Romeo G, Seri M, Deltas C, Abdelhak S
European journal of medical genetics61(1)1-7(2018 Jan)

The genetic and clinical spectrum of a large cohort of patients with distal renal tubular acidosis.
Palazzo V, Provenzano A, Becherucci F, Sansavini G, Mazzinghi B, Orlandini V, Giunti L, Roperto RM, Pantaleo M, Artuso R, Andreucci E, Bargiacchi S, Traficante G, Stagi S, Murer L, Benetti E, Emma F, Giordano M, Rivieri F, Colussi G, Penco S, Manfredini E, Caruso MR, Garavelli L, Andrulli S, Vergine G, Miglietti N, Mancini E, Malaventura C, Percesepe A, Grosso E, Materassi M, Romagnani P, Giglio S
Kidney international91(5)1243-1255(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The aim of this work was to analyze the prevalence of genetic defects in SLC4A1, ATP6V0A4, and ATP6V1B1 genes and to assess the clinical phenotype of distal renal tubular acidosis patients that are eventually typical of the different genetic forms of the disease.
Title: The genetic and clinical spectrum of a large cohort of patients with distal renal tubular acidosis.

Functional analysis of selected regulated proteins revealed that knockdown of HNRPD, PHB2 and UB2V2 can increase HCMV replication, while knockdown of A4 and KSRP resulted in decreased HCMV replication.
Title: Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection.

ITM2A expression is positively regulated by PKA-CREB signaling and ITM2A expression interferes with autophagic flux by interacting with vacuolar ATPase.
Title: The integral membrane protein ITM2A, a transcriptional target of PKA-CREB, regulates autophagic flux via interaction with the vacuolar ATPase.

e have described patients with severe distal renal tubular acidosis and a novel splicing mutation in the ATP6V0A4 gene in a family originating from the Siliana region in northwestern Tunisia
Title: A novel splice-site mutation in ATP6V0A4 gene in two brothers with distal renal tubular acidosis from a consanguineous Tunisian family.

For the remaining patients, two mutations in the ATP6V0A4 gene, one of them being novel, were found in three Tunisian cases.
Title: Molecular investigation of distal renal tubular acidosis in Tunisia, evidence for founder mutations.

The following ATP6V0A4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP6V0A4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15055	NM_020632.3 Latest version!	Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu15055	NM_130841.2 Latest version!	Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu15055	NM_130840.3 Latest version!	Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu15055	XM_005250394.3 Latest version!	Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu15055	XM_005250393.1 Latest version!	Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
View Old Accession Versions >>
OHu15055	NM_020632.2	Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu15055	NM_130840.2	Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP6V0A4 ( NM_020632.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065683.2 Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15055
Clone ID Related Accession (Same CDS sequence)	XM_005250393.1 , XM_005250394.3 , NM_130841.2 , NM_020632.3 , NM_020632.2 , NM_130840.3 , NM_130840.2
Accession Version	NM_020632.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	V-type proton ATPase 116 kDa subunit a isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB230144.1, BC109305.1, AF245517.1 and BC109304.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_020632.2. Transcript Variant: This variant (1) is the longest transcript. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292945.1, AF245517.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968832, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000310018.7/ ENSP00000308122.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGTCTG TGTTTCGAAG CGAGGAGATG TGTTTGTCAC AACTGTTTCT CCAGGTGGAA 
GCTGCATATT GCTGTGTGGC TGAGCTCGGA GAGCTCGGAT TGGTTCAGTT CAAAGATTTA 
AATATGAATG TGAACAGCTT TCAAAGGAAA TTTGTGAATG AAGTCAGAAG GTGTGAATCA 
CTGGAGAGAA TCCTCCGTTT TCTGGAAGAC GAGATGCAAA ATGAGATTGT AGTTCAGTTG 
CTCGAGAAAA GCCCACTGAC CCCGCTCCCA CGGGAAATGA TTACCCTGGA GACTGTTCTA 
GAAAAACTGG AAGGAGAGTT ACAGGAAGCC AACCAGAACC AGCAGGCCTT GAAACAAAGC 
TTCCTAGAAC TGACAGAACT GAAATACCTC CTGAAGAAAA CCCAAGACTT CTTTGAGACG 
GAAACCAATT TAGCTGATGA TTTCTTTACT GAGGACACTT CTGGCCTCCT GGAGTTGAAA 
GCAGTGCCTG CATATATGAC CGGAAAGTTG GGGTTCATAG CCGGTGTGAT CAACAGGGAG 
AGGATGGCTT CCTTTGAGCG GTTACTGTGG CGAATCTGCC GAGGAAACGT GTACTTGAAG 
TTCAGTGAGA TGGACGCCCC TCTGGAGGAT CCTGTGACGA AAGAAGAAAT TCAGAAGAAC 
ATATTCATCA TATTTTACCA AGGAGAGCAG CTCAGGCAGA AAATCAAGAA GATCTGTGAT 
GGGTTTCGAG CCACTGTCTA CCCTTGCCCA GAGCCTGCGG TGGAGCGCAG AGAGATGTTG 
GAGAGCGTCA ATGTGAGGCT GGAAGATTTA ATCACCGTCA TAACACAAAC AGAGTCTCAC 
CGCCAGCGCC TGCTGCAGGA AGCCGCTGCC AACTGGCACT CCTGGCTCAT CAAGGTGCAG 
AAGATGAAAG CTGTCTACCA CATCCTGAAC ATGTGCAACA TCGACGTCAC CCAGCAGTGT 
GTCATCGCCG AGATCTGGTT CCCGGTGGCA GATGCCACAC GTATCAAGAG GGCACTGGAG 
CAAGGCATGG AACTAAGTGG CTCCTCCATG GCCCCCATCA TGACCACAGT GCAATCTAAA 
ACAGCCCCTC CCACATTTAA CAGGACCAAT AAATTCACAG CTGGCTTCCA GAATATTGTT 
GATGCCTATG GTGTCGGCAG CTACCGGGAG ATAAACCCAG CCCCCTACAC CATCATCACT 
TTCCCCTTCC TGTTCGCTGT GATGTTTGGA GACTGTGGTC ATGGAACCGT GATGCTCCTG 
GCTGCACTTT GGATGATTCT GAATGAGAGA CGCTTGCTCT CCCAGAAGAC AGACAATGAG 
ATTTGGAACA CCTTCTTCCA CGGGCGCTAT CTGATCCTAC TTATGGGCAT CTTCTCCATC 
TACACGGGTT TGATCTACAA TGACTGCTTC TCCAAGTCCT TGAACATCTT TGGCTCTTCT 
TGGAGTGTCC AACCCATGTT CAGAAACGGC ACATGGAATA CTCATGTAAT GGAGGAAAGT 
CTATATCTGC AGCTGGACCC AGCCATACCA GGAGTGTATT TTGGAAATCC ATACCCGTTT 
GGGATTGATC CGATTTGGAA CTTGGCTTCA AACAAACTCA CATTTCTGAA CTCGTATAAA 
ATGAAGATGT CGGTGATCCT GGGAATTGTC CAGATGGTTT TCGGTGTCAT CCTCAGCCTT 
TTCAATCACA TATACTTCAG AAGAACTCTC AACATCATTC TGCAATTTAT CCCTGAGATG 
ATTTTTATCC TGTGTCTGTT TGGATACCTG GTTTTCATGA TCATTTTCAA ATGGTGCTGC 
TTTGACGTCC ATGTATCTCA GCACGCCCCC AGCATCCTCA TCCACTTCAT CAACATGTTT 
CTGTTTAACT ACAGTGACTC TTCCAACGCA CCCCTCTACA AACATCAGCA AGAAGTCCAA 
AGTTTCTTTG TGGTTATGGC TTTGATTTCT GTGCCGTGGA TGCTTCTGAT TAAGCCGTTT 
ATTCTTAGAG CCAGTCATCG GAAATCCCAG CTGCAGGCAT CCAGGATCCA AGAAGATGCC 
ACTGAGAACA TTGAAGGTGA TAGCTCCAGC CCTTCTAGCC GTTCTGGCCA GAGGACTTCT 
GCAGATACCC ACGGGGCTCT GGACGACCAT GGAGAAGAGT TCAACTTTGG AGACGTCTTT 
GTCCACCAAG CCATCCACAC CATCGAGTAC TGCCTGGGCT GCATTTCAAA CACAGCCTCC 
TACCTGCGGC TCTGGGCCCT CAGCCTGGCT CATGCACAAC TGTCTGAAGT GCTCTGGACT 
ATGGTGATGA ACAGCGGCCT TCAGACGCGA GGCTGGGGAG GAATCGTCGG GGTTTTTATT 
ATTTTTGCCG TATTTGCTGT CCTGACAGTA GCCATCCTTC TGATCATGGA GGGCCTCTCT 
GCTTTCCTGC ACGCCCTGCG ACTGCACTGG GTTGAGTTCC AGAACAAGTT CTATGTCGGG 
GATGGTTACA AGTTTTCTCC ATTCTCCTTT AAACACATCC TGGATGGCAC AGCCGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065683.2
CDS	284..2806
Translation	MVSVFRSEEMCLSQLFLQVEAAYCCVAELGELGLVQFKDLNMNVNSFQRKFVNEVRRCESLERILRFLEDEMQNEIVVQLLEKSPLTPLPREMITLETVLEKLEGELQEANQNQQALKQSFLELTELKYLLKKTQDFFETETNLADDFFTEDTSGLLELKAVPAYMTGKLGFIAGVINRERMASFERLLWRICRGNVYLKFSEMDAPLEDPVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPEPAVERREMLESVNVRLEDLITVITQTESHRQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAEIWFPVADATRIKRALEQGMELSGSSMAPIMTTVQSKTAPPTFNRTNKFTAGFQNIVDAYGVGSYREINPAPYTIITFPFLFAVMFGDCGHGTVMLLAALWMILNERRLLSQKTDNEIWNTFFHGRYLILLMGIFSIYTGLIYNDCFSKSLNIFGSSWSVQPMFRNGTWNTHVMEESLYLQLDPAIPGVYFGNPYPFGIDPIWNLASNKLTFLNSYKMKMSVILGIVQMVFGVILSLFNHIYFRRTLNIILQFIPEMIFILCLFGYLVFMIIFKWCCFDVHVSQHAPSILIHFINMFLFNYSDSSNAPLYKHQQEVQSFFVVMALISVPWMLLIKPFILRASHRKSQLQASRIQEDATENIEGDSSSPSSRSGQRTSADTHGALDDHGEEFNFGDVFVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVMNSGLQTRGWGGIVGVFIIFAVFAVLTVAILLIMEGLSAFLHALRLHWVEFQNKFYVGDGYKFSPFSFKHILDGTAEE

Target ORF information:

RefSeq Version	NM_020632.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020632.3

ORF Insert Sequence:

ATGGTGTCTG TGTTTCGAAG CGAGGAGATG TGTTTGTCAC AACTGTTTCT CCAGGTGGAA 
GCTGCATATT GCTGTGTGGC TGAGCTCGGA GAGCTCGGAT TGGTTCAGTT CAAAGATTTA 
AATATGAATG TGAACAGCTT TCAAAGGAAA TTTGTGAATG AAGTCAGAAG GTGTGAATCA 
CTGGAGAGAA TCCTCCGTTT TCTGGAAGAC GAGATGCAAA ATGAGATTGT AGTTCAGTTG 
CTCGAGAAAA GCCCACTGAC CCCGCTCCCA CGGGAAATGA TTACCCTGGA GACTGTTCTA 
GAAAAACTGG AAGGAGAGTT ACAGGAAGCC AACCAGAACC AGCAGGCCTT GAAACAAAGC 
TTCCTAGAAC TGACAGAACT GAAATACCTC CTGAAGAAAA CCCAAGACTT CTTTGAGACG 
GAAACCAATT TAGCTGATGA TTTCTTTACT GAGGACACTT CTGGCCTCCT GGAGTTGAAA 
GCAGTGCCTG CATATATGAC CGGAAAGTTG GGGTTCATAG CCGGTGTGAT CAACAGGGAG 
AGGATGGCTT CCTTTGAGCG GTTACTGTGG CGAATCTGCC GAGGAAACGT GTACTTGAAG 
TTCAGTGAGA TGGACGCCCC TCTGGAGGAT CCTGTGACGA AAGAAGAAAT TCAGAAGAAC 
ATATTCATCA TATTTTACCA AGGAGAGCAG CTCAGGCAGA AAATCAAGAA GATCTGTGAT 
GGGTTTCGAG CCACTGTCTA CCCTTGCCCA GAGCCTGCGG TGGAGCGCAG AGAGATGTTG 
GAGAGCGTCA ATGTGAGGCT GGAAGATTTA ATCACCGTCA TAACACAAAC AGAGTCTCAC 
CGCCAGCGCC TGCTGCAGGA AGCCGCTGCC AACTGGCACT CCTGGCTCAT CAAGGTGCAG 
AAGATGAAAG CTGTCTACCA CATCCTGAAC ATGTGCAACA TCGACGTCAC CCAGCAGTGT 
GTCATCGCCG AGATCTGGTT CCCGGTGGCA GATGCCACAC GTATCAAGAG GGCACTGGAG 
CAAGGCATGG AACTAAGTGG CTCCTCCATG GCCCCCATCA TGACCACAGT GCAATCTAAA 
ACAGCCCCTC CCACATTTAA CAGGACCAAT AAATTCACAG CTGGCTTCCA GAATATTGTT 
GATGCCTATG GTGTCGGCAG CTACCGGGAG ATAAACCCAG CCCCCTACAC CATCATCACT 
TTCCCCTTCC TGTTCGCTGT GATGTTTGGA GACTGTGGTC ATGGAACCGT GATGCTCCTG 
GCTGCACTTT GGATGATTCT GAATGAGAGA CGCTTGCTCT CCCAGAAGAC AGACAATGAG 
ATTTGGAACA CCTTCTTCCA CGGGCGCTAT CTGATCCTAC TTATGGGCAT CTTCTCCATC 
TACACGGGTT TGATCTACAA TGACTGCTTC TCCAAGTCCT TGAACATCTT TGGCTCTTCT 
TGGAGTGTCC AACCCATGTT CAGAAACGGC ACATGGAATA CTCATGTAAT GGAGGAAAGT 
CTATATCTGC AGCTGGACCC AGCCATACCA GGAGTGTATT TTGGAAATCC ATACCCGTTT 
GGGATTGATC CGATTTGGAA CTTGGCTTCA AACAAACTCA CATTTCTGAA CTCGTATAAA 
ATGAAGATGT CGGTGATCCT GGGAATTGTC CAGATGGTTT TCGGTGTCAT CCTCAGCCTT 
TTCAATCACA TATACTTCAG AAGAACTCTC AACATCATTC TGCAATTTAT CCCTGAGATG 
ATTTTTATCC TGTGTCTGTT TGGATACCTG GTTTTCATGA TCATTTTCAA ATGGTGCTGC 
TTTGACGTCC ATGTATCTCA GCACGCCCCC AGCATCCTCA TCCACTTCAT CAACATGTTT 
CTGTTTAACT ACAGTGACTC TTCCAACGCA CCCCTCTACA AACATCAGCA AGAAGTCCAA 
AGTTTCTTTG TGGTTATGGC TTTGATTTCT GTGCCGTGGA TGCTTCTGAT TAAGCCGTTT 
ATTCTTAGAG CCAGTCATCG GAAATCCCAG CTGCAGGCAT CCAGGATCCA AGAAGATGCC 
ACTGAGAACA TTGAAGGTGA TAGCTCCAGC CCTTCTAGCC GTTCTGGCCA GAGGACTTCT 
GCAGATACCC ACGGGGCTCT GGACGACCAT GGAGAAGAGT TCAACTTTGG AGACGTCTTT 
GTCCACCAAG CCATCCACAC CATCGAGTAC TGCCTGGGCT GCATTTCAAA CACAGCCTCC 
TACCTGCGGC TCTGGGCCCT CAGCCTGGCT CATGCACAAC TGTCTGAAGT GCTCTGGACT 
ATGGTGATGA ACAGCGGCCT TCAGACGCGA GGCTGGGGAG GAATCGTCGG GGTTTTTATT 
ATTTTTGCCG TATTTGCTGT CCTGACAGTA GCCATCCTTC TGATCATGGA GGGCCTCTCT 
GCTTTCCTGC ACGCCCTGCG ACTGCACTGG GTTGAGTTCC AGAACAAGTT CTATGTCGGG 
GATGGTTACA AGTTTTCTCC ATTCTCCTTT AAACACATCC TGGATGGCAC AGCCGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V0A4 ( NM_130841.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_570856.2 Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu15055
Clone ID Related Accession (Same CDS sequence)	XM_005250393.1 , XM_005250394.3 , NM_130841.2 , NM_020632.3 , NM_020632.2 , NM_130840.3 , NM_130840.2
Accession Version	NM_130841.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	V-type proton ATPase 116 kDa subunit a isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA857655.1, BC109305.1, AF245517.1 and BC109304.1. On Jan 19, 2006 this sequence version replaced NM_130841.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF245517.1, AK292945.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1968832, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGTCTG TGTTTCGAAG CGAGGAGATG TGTTTGTCAC AACTGTTTCT CCAGGTGGAA 
GCTGCATATT GCTGTGTGGC TGAGCTCGGA GAGCTCGGAT TGGTTCAGTT CAAAGATTTA 
AATATGAATG TGAACAGCTT TCAAAGGAAA TTTGTGAATG AAGTCAGAAG GTGTGAATCA 
CTGGAGAGAA TCCTCCGTTT TCTGGAAGAC GAGATGCAAA ATGAGATTGT AGTTCAGTTG 
CTCGAGAAAA GCCCACTGAC CCCGCTCCCA CGGGAAATGA TTACCCTGGA GACTGTTCTA 
GAAAAACTGG AAGGAGAGTT ACAGGAAGCC AACCAGAACC AGCAGGCCTT GAAACAAAGC 
TTCCTAGAAC TGACAGAACT GAAATACCTC CTGAAGAAAA CCCAAGACTT CTTTGAGACG 
GAAACCAATT TAGCTGATGA TTTCTTTACT GAGGACACTT CTGGCCTCCT GGAGTTGAAA 
GCAGTGCCTG CATATATGAC CGGAAAGTTG GGGTTCATAG CCGGTGTGAT CAACAGGGAG 
AGGATGGCTT CCTTTGAGCG GTTACTGTGG CGAATCTGCC GAGGAAACGT GTACTTGAAG 
TTCAGTGAGA TGGACGCCCC TCTGGAGGAT CCTGTGACGA AAGAAGAAAT TCAGAAGAAC 
ATATTCATCA TATTTTACCA AGGAGAGCAG CTCAGGCAGA AAATCAAGAA GATCTGTGAT 
GGGTTTCGAG CCACTGTCTA CCCTTGCCCA GAGCCTGCGG TGGAGCGCAG AGAGATGTTG 
GAGAGCGTCA ATGTGAGGCT GGAAGATTTA ATCACCGTCA TAACACAAAC AGAGTCTCAC 
CGCCAGCGCC TGCTGCAGGA AGCCGCTGCC AACTGGCACT CCTGGCTCAT CAAGGTGCAG 
AAGATGAAAG CTGTCTACCA CATCCTGAAC ATGTGCAACA TCGACGTCAC CCAGCAGTGT 
GTCATCGCCG AGATCTGGTT CCCGGTGGCA GATGCCACAC GTATCAAGAG GGCACTGGAG 
CAAGGCATGG AACTAAGTGG CTCCTCCATG GCCCCCATCA TGACCACAGT GCAATCTAAA 
ACAGCCCCTC CCACATTTAA CAGGACCAAT AAATTCACAG CTGGCTTCCA GAATATTGTT 
GATGCCTATG GTGTCGGCAG CTACCGGGAG ATAAACCCAG CCCCCTACAC CATCATCACT 
TTCCCCTTCC TGTTCGCTGT GATGTTTGGA GACTGTGGTC ATGGAACCGT GATGCTCCTG 
GCTGCACTTT GGATGATTCT GAATGAGAGA CGCTTGCTCT CCCAGAAGAC AGACAATGAG 
ATTTGGAACA CCTTCTTCCA CGGGCGCTAT CTGATCCTAC TTATGGGCAT CTTCTCCATC 
TACACGGGTT TGATCTACAA TGACTGCTTC TCCAAGTCCT TGAACATCTT TGGCTCTTCT 
TGGAGTGTCC AACCCATGTT CAGAAACGGC ACATGGAATA CTCATGTAAT GGAGGAAAGT 
CTATATCTGC AGCTGGACCC AGCCATACCA GGAGTGTATT TTGGAAATCC ATACCCGTTT 
GGGATTGATC CGATTTGGAA CTTGGCTTCA AACAAACTCA CATTTCTGAA CTCGTATAAA 
ATGAAGATGT CGGTGATCCT GGGAATTGTC CAGATGGTTT TCGGTGTCAT CCTCAGCCTT 
TTCAATCACA TATACTTCAG AAGAACTCTC AACATCATTC TGCAATTTAT CCCTGAGATG 
ATTTTTATCC TGTGTCTGTT TGGATACCTG GTTTTCATGA TCATTTTCAA ATGGTGCTGC 
TTTGACGTCC ATGTATCTCA GCACGCCCCC AGCATCCTCA TCCACTTCAT CAACATGTTT 
CTGTTTAACT ACAGTGACTC TTCCAACGCA CCCCTCTACA AACATCAGCA AGAAGTCCAA 
AGTTTCTTTG TGGTTATGGC TTTGATTTCT GTGCCGTGGA TGCTTCTGAT TAAGCCGTTT 
ATTCTTAGAG CCAGTCATCG GAAATCCCAG CTGCAGGCAT CCAGGATCCA AGAAGATGCC 
ACTGAGAACA TTGAAGGTGA TAGCTCCAGC CCTTCTAGCC GTTCTGGCCA GAGGACTTCT 
GCAGATACCC ACGGGGCTCT GGACGACCAT GGAGAAGAGT TCAACTTTGG AGACGTCTTT 
GTCCACCAAG CCATCCACAC CATCGAGTAC TGCCTGGGCT GCATTTCAAA CACAGCCTCC 
TACCTGCGGC TCTGGGCCCT CAGCCTGGCT CATGCACAAC TGTCTGAAGT GCTCTGGACT 
ATGGTGATGA ACAGCGGCCT TCAGACGCGA GGCTGGGGAG GAATCGTCGG GGTTTTTATT 
ATTTTTGCCG TATTTGCTGT CCTGACAGTA GCCATCCTTC TGATCATGGA GGGCCTCTCT 
GCTTTCCTGC ACGCCCTGCG ACTGCACTGG GTTGAGTTCC AGAACAAGTT CTATGTCGGG 
GATGGTTACA AGTTTTCTCC ATTCTCCTTT AAACACATCC TGGATGGCAC AGCCGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570856.2
CDS	239..2761
Translation	MVSVFRSEEMCLSQLFLQVEAAYCCVAELGELGLVQFKDLNMNVNSFQRKFVNEVRRCESLERILRFLEDEMQNEIVVQLLEKSPLTPLPREMITLETVLEKLEGELQEANQNQQALKQSFLELTELKYLLKKTQDFFETETNLADDFFTEDTSGLLELKAVPAYMTGKLGFIAGVINRERMASFERLLWRICRGNVYLKFSEMDAPLEDPVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPEPAVERREMLESVNVRLEDLITVITQTESHRQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAEIWFPVADATRIKRALEQGMELSGSSMAPIMTTVQSKTAPPTFNRTNKFTAGFQNIVDAYGVGSYREINPAPYTIITFPFLFAVMFGDCGHGTVMLLAALWMILNERRLLSQKTDNEIWNTFFHGRYLILLMGIFSIYTGLIYNDCFSKSLNIFGSSWSVQPMFRNGTWNTHVMEESLYLQLDPAIPGVYFGNPYPFGIDPIWNLASNKLTFLNSYKMKMSVILGIVQMVFGVILSLFNHIYFRRTLNIILQFIPEMIFILCLFGYLVFMIIFKWCCFDVHVSQHAPSILIHFINMFLFNYSDSSNAPLYKHQQEVQSFFVVMALISVPWMLLIKPFILRASHRKSQLQASRIQEDATENIEGDSSSPSSRSGQRTSADTHGALDDHGEEFNFGDVFVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVMNSGLQTRGWGGIVGVFIIFAVFAVLTVAILLIMEGLSAFLHALRLHWVEFQNKFYVGDGYKFSPFSFKHILDGTAEE

Target ORF information:

RefSeq Version	NM_130841.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130841.2

ORF Insert Sequence:

ATGGTGTCTG TGTTTCGAAG CGAGGAGATG TGTTTGTCAC AACTGTTTCT CCAGGTGGAA 
GCTGCATATT GCTGTGTGGC TGAGCTCGGA GAGCTCGGAT TGGTTCAGTT CAAAGATTTA 
AATATGAATG TGAACAGCTT TCAAAGGAAA TTTGTGAATG AAGTCAGAAG GTGTGAATCA 
CTGGAGAGAA TCCTCCGTTT TCTGGAAGAC GAGATGCAAA ATGAGATTGT AGTTCAGTTG 
CTCGAGAAAA GCCCACTGAC CCCGCTCCCA CGGGAAATGA TTACCCTGGA GACTGTTCTA 
GAAAAACTGG AAGGAGAGTT ACAGGAAGCC AACCAGAACC AGCAGGCCTT GAAACAAAGC 
TTCCTAGAAC TGACAGAACT GAAATACCTC CTGAAGAAAA CCCAAGACTT CTTTGAGACG 
GAAACCAATT TAGCTGATGA TTTCTTTACT GAGGACACTT CTGGCCTCCT GGAGTTGAAA 
GCAGTGCCTG CATATATGAC CGGAAAGTTG GGGTTCATAG CCGGTGTGAT CAACAGGGAG 
AGGATGGCTT CCTTTGAGCG GTTACTGTGG CGAATCTGCC GAGGAAACGT GTACTTGAAG 
TTCAGTGAGA TGGACGCCCC TCTGGAGGAT CCTGTGACGA AAGAAGAAAT TCAGAAGAAC 
ATATTCATCA TATTTTACCA AGGAGAGCAG CTCAGGCAGA AAATCAAGAA GATCTGTGAT 
GGGTTTCGAG CCACTGTCTA CCCTTGCCCA GAGCCTGCGG TGGAGCGCAG AGAGATGTTG 
GAGAGCGTCA ATGTGAGGCT GGAAGATTTA ATCACCGTCA TAACACAAAC AGAGTCTCAC 
CGCCAGCGCC TGCTGCAGGA AGCCGCTGCC AACTGGCACT CCTGGCTCAT CAAGGTGCAG 
AAGATGAAAG CTGTCTACCA CATCCTGAAC ATGTGCAACA TCGACGTCAC CCAGCAGTGT 
GTCATCGCCG AGATCTGGTT CCCGGTGGCA GATGCCACAC GTATCAAGAG GGCACTGGAG 
CAAGGCATGG AACTAAGTGG CTCCTCCATG GCCCCCATCA TGACCACAGT GCAATCTAAA 
ACAGCCCCTC CCACATTTAA CAGGACCAAT AAATTCACAG CTGGCTTCCA GAATATTGTT 
GATGCCTATG GTGTCGGCAG CTACCGGGAG ATAAACCCAG CCCCCTACAC CATCATCACT 
TTCCCCTTCC TGTTCGCTGT GATGTTTGGA GACTGTGGTC ATGGAACCGT GATGCTCCTG 
GCTGCACTTT GGATGATTCT GAATGAGAGA CGCTTGCTCT CCCAGAAGAC AGACAATGAG 
ATTTGGAACA CCTTCTTCCA CGGGCGCTAT CTGATCCTAC TTATGGGCAT CTTCTCCATC 
TACACGGGTT TGATCTACAA TGACTGCTTC TCCAAGTCCT TGAACATCTT TGGCTCTTCT 
TGGAGTGTCC AACCCATGTT CAGAAACGGC ACATGGAATA CTCATGTAAT GGAGGAAAGT 
CTATATCTGC AGCTGGACCC AGCCATACCA GGAGTGTATT TTGGAAATCC ATACCCGTTT 
GGGATTGATC CGATTTGGAA CTTGGCTTCA AACAAACTCA CATTTCTGAA CTCGTATAAA 
ATGAAGATGT CGGTGATCCT GGGAATTGTC CAGATGGTTT TCGGTGTCAT CCTCAGCCTT 
TTCAATCACA TATACTTCAG AAGAACTCTC AACATCATTC TGCAATTTAT CCCTGAGATG 
ATTTTTATCC TGTGTCTGTT TGGATACCTG GTTTTCATGA TCATTTTCAA ATGGTGCTGC 
TTTGACGTCC ATGTATCTCA GCACGCCCCC AGCATCCTCA TCCACTTCAT CAACATGTTT 
CTGTTTAACT ACAGTGACTC TTCCAACGCA CCCCTCTACA AACATCAGCA AGAAGTCCAA 
AGTTTCTTTG TGGTTATGGC TTTGATTTCT GTGCCGTGGA TGCTTCTGAT TAAGCCGTTT 
ATTCTTAGAG CCAGTCATCG GAAATCCCAG CTGCAGGCAT CCAGGATCCA AGAAGATGCC 
ACTGAGAACA TTGAAGGTGA TAGCTCCAGC CCTTCTAGCC GTTCTGGCCA GAGGACTTCT 
GCAGATACCC ACGGGGCTCT GGACGACCAT GGAGAAGAGT TCAACTTTGG AGACGTCTTT 
GTCCACCAAG CCATCCACAC CATCGAGTAC TGCCTGGGCT GCATTTCAAA CACAGCCTCC 
TACCTGCGGC TCTGGGCCCT CAGCCTGGCT CATGCACAAC TGTCTGAAGT GCTCTGGACT 
ATGGTGATGA ACAGCGGCCT TCAGACGCGA GGCTGGGGAG GAATCGTCGG GGTTTTTATT 
ATTTTTGCCG TATTTGCTGT CCTGACAGTA GCCATCCTTC TGATCATGGA GGGCCTCTCT 
GCTTTCCTGC ACGCCCTGCG ACTGCACTGG GTTGAGTTCC AGAACAAGTT CTATGTCGGG 
GATGGTTACA AGTTTTCTCC ATTCTCCTTT AAACACATCC TGGATGGCAC AGCCGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V0A4 ( NM_020632.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065683.2 Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant 1, mRNA.

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CloneID	OHu15055
Clone ID Related Accession (Same CDS sequence)	XM_005250393.1 , XM_005250394.3 , NM_130841.2 , NM_020632.3 , NM_020632.2 , NM_130840.3 , NM_130840.2
Accession Version	NM_020632.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	V-type proton ATPase 116 kDa subunit a isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB230144.1, BC109305.1, AF245517.1 and BC109304.1. This sequence is a reference standard in the RefSeqGene project. On Jan 19, 2006 this sequence version replaced NM_020632.1. Transcript Variant: This variant (1) is the longest transcript. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF245517.1, AK292945.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968832, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGTCTG TGTTTCGAAG CGAGGAGATG TGTTTGTCAC AACTGTTTCT CCAGGTGGAA 
GCTGCATATT GCTGTGTGGC TGAGCTCGGA GAGCTCGGAT TGGTTCAGTT CAAAGATTTA 
AATATGAATG TGAACAGCTT TCAAAGGAAA TTTGTGAATG AAGTCAGAAG GTGTGAATCA 
CTGGAGAGAA TCCTCCGTTT TCTGGAAGAC GAGATGCAAA ATGAGATTGT AGTTCAGTTG 
CTCGAGAAAA GCCCACTGAC CCCGCTCCCA CGGGAAATGA TTACCCTGGA GACTGTTCTA 
GAAAAACTGG AAGGAGAGTT ACAGGAAGCC AACCAGAACC AGCAGGCCTT GAAACAAAGC 
TTCCTAGAAC TGACAGAACT GAAATACCTC CTGAAGAAAA CCCAAGACTT CTTTGAGACG 
GAAACCAATT TAGCTGATGA TTTCTTTACT GAGGACACTT CTGGCCTCCT GGAGTTGAAA 
GCAGTGCCTG CATATATGAC CGGAAAGTTG GGGTTCATAG CCGGTGTGAT CAACAGGGAG 
AGGATGGCTT CCTTTGAGCG GTTACTGTGG CGAATCTGCC GAGGAAACGT GTACTTGAAG 
TTCAGTGAGA TGGACGCCCC TCTGGAGGAT CCTGTGACGA AAGAAGAAAT TCAGAAGAAC 
ATATTCATCA TATTTTACCA AGGAGAGCAG CTCAGGCAGA AAATCAAGAA GATCTGTGAT 
GGGTTTCGAG CCACTGTCTA CCCTTGCCCA GAGCCTGCGG TGGAGCGCAG AGAGATGTTG 
GAGAGCGTCA ATGTGAGGCT GGAAGATTTA ATCACCGTCA TAACACAAAC AGAGTCTCAC 
CGCCAGCGCC TGCTGCAGGA AGCCGCTGCC AACTGGCACT CCTGGCTCAT CAAGGTGCAG 
AAGATGAAAG CTGTCTACCA CATCCTGAAC ATGTGCAACA TCGACGTCAC CCAGCAGTGT 
GTCATCGCCG AGATCTGGTT CCCGGTGGCA GATGCCACAC GTATCAAGAG GGCACTGGAG 
CAAGGCATGG AACTAAGTGG CTCCTCCATG GCCCCCATCA TGACCACAGT GCAATCTAAA 
ACAGCCCCTC CCACATTTAA CAGGACCAAT AAATTCACAG CTGGCTTCCA GAATATTGTT 
GATGCCTATG GTGTCGGCAG CTACCGGGAG ATAAACCCAG CCCCCTACAC CATCATCACT 
TTCCCCTTCC TGTTCGCTGT GATGTTTGGA GACTGTGGTC ATGGAACCGT GATGCTCCTG 
GCTGCACTTT GGATGATTCT GAATGAGAGA CGCTTGCTCT CCCAGAAGAC AGACAATGAG 
ATTTGGAACA CCTTCTTCCA CGGGCGCTAT CTGATCCTAC TTATGGGCAT CTTCTCCATC 
TACACGGGTT TGATCTACAA TGACTGCTTC TCCAAGTCCT TGAACATCTT TGGCTCTTCT 
TGGAGTGTCC AACCCATGTT CAGAAACGGC ACATGGAATA CTCATGTAAT GGAGGAAAGT 
CTATATCTGC AGCTGGACCC AGCCATACCA GGAGTGTATT TTGGAAATCC ATACCCGTTT 
GGGATTGATC CGATTTGGAA CTTGGCTTCA AACAAACTCA CATTTCTGAA CTCGTATAAA 
ATGAAGATGT CGGTGATCCT GGGAATTGTC CAGATGGTTT TCGGTGTCAT CCTCAGCCTT 
TTCAATCACA TATACTTCAG AAGAACTCTC AACATCATTC TGCAATTTAT CCCTGAGATG 
ATTTTTATCC TGTGTCTGTT TGGATACCTG GTTTTCATGA TCATTTTCAA ATGGTGCTGC 
TTTGACGTCC ATGTATCTCA GCACGCCCCC AGCATCCTCA TCCACTTCAT CAACATGTTT 
CTGTTTAACT ACAGTGACTC TTCCAACGCA CCCCTCTACA AACATCAGCA AGAAGTCCAA 
AGTTTCTTTG TGGTTATGGC TTTGATTTCT GTGCCGTGGA TGCTTCTGAT TAAGCCGTTT 
ATTCTTAGAG CCAGTCATCG GAAATCCCAG CTGCAGGCAT CCAGGATCCA AGAAGATGCC 
ACTGAGAACA TTGAAGGTGA TAGCTCCAGC CCTTCTAGCC GTTCTGGCCA GAGGACTTCT 
GCAGATACCC ACGGGGCTCT GGACGACCAT GGAGAAGAGT TCAACTTTGG AGACGTCTTT 
GTCCACCAAG CCATCCACAC CATCGAGTAC TGCCTGGGCT GCATTTCAAA CACAGCCTCC 
TACCTGCGGC TCTGGGCCCT CAGCCTGGCT CATGCACAAC TGTCTGAAGT GCTCTGGACT 
ATGGTGATGA ACAGCGGCCT TCAGACGCGA GGCTGGGGAG GAATCGTCGG GGTTTTTATT 
ATTTTTGCCG TATTTGCTGT CCTGACAGTA GCCATCCTTC TGATCATGGA GGGCCTCTCT 
GCTTTCCTGC ACGCCCTGCG ACTGCACTGG GTTGAGTTCC AGAACAAGTT CTATGTCGGG 
GATGGTTACA AGTTTTCTCC ATTCTCCTTT AAACACATCC TGGATGGCAC AGCCGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065683.2
CDS	284..2806
Translation	MVSVFRSEEMCLSQLFLQVEAAYCCVAELGELGLVQFKDLNMNVNSFQRKFVNEVRRCESLERILRFLEDEMQNEIVVQLLEKSPLTPLPREMITLETVLEKLEGELQEANQNQQALKQSFLELTELKYLLKKTQDFFETETNLADDFFTEDTSGLLELKAVPAYMTGKLGFIAGVINRERMASFERLLWRICRGNVYLKFSEMDAPLEDPVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPEPAVERREMLESVNVRLEDLITVITQTESHRQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAEIWFPVADATRIKRALEQGMELSGSSMAPIMTTVQSKTAPPTFNRTNKFTAGFQNIVDAYGVGSYREINPAPYTIITFPFLFAVMFGDCGHGTVMLLAALWMILNERRLLSQKTDNEIWNTFFHGRYLILLMGIFSIYTGLIYNDCFSKSLNIFGSSWSVQPMFRNGTWNTHVMEESLYLQLDPAIPGVYFGNPYPFGIDPIWNLASNKLTFLNSYKMKMSVILGIVQMVFGVILSLFNHIYFRRTLNIILQFIPEMIFILCLFGYLVFMIIFKWCCFDVHVSQHAPSILIHFINMFLFNYSDSSNAPLYKHQQEVQSFFVVMALISVPWMLLIKPFILRASHRKSQLQASRIQEDATENIEGDSSSPSSRSGQRTSADTHGALDDHGEEFNFGDVFVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVMNSGLQTRGWGGIVGVFIIFAVFAVLTVAILLIMEGLSAFLHALRLHWVEFQNKFYVGDGYKFSPFSFKHILDGTAEE

Target ORF information:

RefSeq Version	NM_020632.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020632.2

ORF Insert Sequence:

ATGGTGTCTG TGTTTCGAAG CGAGGAGATG TGTTTGTCAC AACTGTTTCT CCAGGTGGAA 
GCTGCATATT GCTGTGTGGC TGAGCTCGGA GAGCTCGGAT TGGTTCAGTT CAAAGATTTA 
AATATGAATG TGAACAGCTT TCAAAGGAAA TTTGTGAATG AAGTCAGAAG GTGTGAATCA 
CTGGAGAGAA TCCTCCGTTT TCTGGAAGAC GAGATGCAAA ATGAGATTGT AGTTCAGTTG 
CTCGAGAAAA GCCCACTGAC CCCGCTCCCA CGGGAAATGA TTACCCTGGA GACTGTTCTA 
GAAAAACTGG AAGGAGAGTT ACAGGAAGCC AACCAGAACC AGCAGGCCTT GAAACAAAGC 
TTCCTAGAAC TGACAGAACT GAAATACCTC CTGAAGAAAA CCCAAGACTT CTTTGAGACG 
GAAACCAATT TAGCTGATGA TTTCTTTACT GAGGACACTT CTGGCCTCCT GGAGTTGAAA 
GCAGTGCCTG CATATATGAC CGGAAAGTTG GGGTTCATAG CCGGTGTGAT CAACAGGGAG 
AGGATGGCTT CCTTTGAGCG GTTACTGTGG CGAATCTGCC GAGGAAACGT GTACTTGAAG 
TTCAGTGAGA TGGACGCCCC TCTGGAGGAT CCTGTGACGA AAGAAGAAAT TCAGAAGAAC 
ATATTCATCA TATTTTACCA AGGAGAGCAG CTCAGGCAGA AAATCAAGAA GATCTGTGAT 
GGGTTTCGAG CCACTGTCTA CCCTTGCCCA GAGCCTGCGG TGGAGCGCAG AGAGATGTTG 
GAGAGCGTCA ATGTGAGGCT GGAAGATTTA ATCACCGTCA TAACACAAAC AGAGTCTCAC 
CGCCAGCGCC TGCTGCAGGA AGCCGCTGCC AACTGGCACT CCTGGCTCAT CAAGGTGCAG 
AAGATGAAAG CTGTCTACCA CATCCTGAAC ATGTGCAACA TCGACGTCAC CCAGCAGTGT 
GTCATCGCCG AGATCTGGTT CCCGGTGGCA GATGCCACAC GTATCAAGAG GGCACTGGAG 
CAAGGCATGG AACTAAGTGG CTCCTCCATG GCCCCCATCA TGACCACAGT GCAATCTAAA 
ACAGCCCCTC CCACATTTAA CAGGACCAAT AAATTCACAG CTGGCTTCCA GAATATTGTT 
GATGCCTATG GTGTCGGCAG CTACCGGGAG ATAAACCCAG CCCCCTACAC CATCATCACT 
TTCCCCTTCC TGTTCGCTGT GATGTTTGGA GACTGTGGTC ATGGAACCGT GATGCTCCTG 
GCTGCACTTT GGATGATTCT GAATGAGAGA CGCTTGCTCT CCCAGAAGAC AGACAATGAG 
ATTTGGAACA CCTTCTTCCA CGGGCGCTAT CTGATCCTAC TTATGGGCAT CTTCTCCATC 
TACACGGGTT TGATCTACAA TGACTGCTTC TCCAAGTCCT TGAACATCTT TGGCTCTTCT 
TGGAGTGTCC AACCCATGTT CAGAAACGGC ACATGGAATA CTCATGTAAT GGAGGAAAGT 
CTATATCTGC AGCTGGACCC AGCCATACCA GGAGTGTATT TTGGAAATCC ATACCCGTTT 
GGGATTGATC CGATTTGGAA CTTGGCTTCA AACAAACTCA CATTTCTGAA CTCGTATAAA 
ATGAAGATGT CGGTGATCCT GGGAATTGTC CAGATGGTTT TCGGTGTCAT CCTCAGCCTT 
TTCAATCACA TATACTTCAG AAGAACTCTC AACATCATTC TGCAATTTAT CCCTGAGATG 
ATTTTTATCC TGTGTCTGTT TGGATACCTG GTTTTCATGA TCATTTTCAA ATGGTGCTGC 
TTTGACGTCC ATGTATCTCA GCACGCCCCC AGCATCCTCA TCCACTTCAT CAACATGTTT 
CTGTTTAACT ACAGTGACTC TTCCAACGCA CCCCTCTACA AACATCAGCA AGAAGTCCAA 
AGTTTCTTTG TGGTTATGGC TTTGATTTCT GTGCCGTGGA TGCTTCTGAT TAAGCCGTTT 
ATTCTTAGAG CCAGTCATCG GAAATCCCAG CTGCAGGCAT CCAGGATCCA AGAAGATGCC 
ACTGAGAACA TTGAAGGTGA TAGCTCCAGC CCTTCTAGCC GTTCTGGCCA GAGGACTTCT 
GCAGATACCC ACGGGGCTCT GGACGACCAT GGAGAAGAGT TCAACTTTGG AGACGTCTTT 
GTCCACCAAG CCATCCACAC CATCGAGTAC TGCCTGGGCT GCATTTCAAA CACAGCCTCC 
TACCTGCGGC TCTGGGCCCT CAGCCTGGCT CATGCACAAC TGTCTGAAGT GCTCTGGACT 
ATGGTGATGA ACAGCGGCCT TCAGACGCGA GGCTGGGGAG GAATCGTCGG GGTTTTTATT 
ATTTTTGCCG TATTTGCTGT CCTGACAGTA GCCATCCTTC TGATCATGGA GGGCCTCTCT 
GCTTTCCTGC ACGCCCTGCG ACTGCACTGG GTTGAGTTCC AGAACAAGTT CTATGTCGGG 
GATGGTTACA AGTTTTCTCC ATTCTCCTTT AAACACATCC TGGATGGCAC AGCCGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V0A4 ( NM_130840.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_570855.2 Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15055
Clone ID Related Accession (Same CDS sequence)	XM_005250393.1 , XM_005250394.3 , NM_130841.2 , NM_020632.3 , NM_020632.2 , NM_130840.3 , NM_130840.2
Accession Version	NM_130840.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	V-type proton ATPase 116 kDa subunit a isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB230144.1, BC109305.1, AF245517.1 and BC109304.1. On May 31, 2019 this sequence version replaced NM_130840.2. Transcript Variant: This variant (2) lacks an exon in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC109304.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968832, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGTCTG TGTTTCGAAG CGAGGAGATG TGTTTGTCAC AACTGTTTCT CCAGGTGGAA 
GCTGCATATT GCTGTGTGGC TGAGCTCGGA GAGCTCGGAT TGGTTCAGTT CAAAGATTTA 
AATATGAATG TGAACAGCTT TCAAAGGAAA TTTGTGAATG AAGTCAGAAG GTGTGAATCA 
CTGGAGAGAA TCCTCCGTTT TCTGGAAGAC GAGATGCAAA ATGAGATTGT AGTTCAGTTG 
CTCGAGAAAA GCCCACTGAC CCCGCTCCCA CGGGAAATGA TTACCCTGGA GACTGTTCTA 
GAAAAACTGG AAGGAGAGTT ACAGGAAGCC AACCAGAACC AGCAGGCCTT GAAACAAAGC 
TTCCTAGAAC TGACAGAACT GAAATACCTC CTGAAGAAAA CCCAAGACTT CTTTGAGACG 
GAAACCAATT TAGCTGATGA TTTCTTTACT GAGGACACTT CTGGCCTCCT GGAGTTGAAA 
GCAGTGCCTG CATATATGAC CGGAAAGTTG GGGTTCATAG CCGGTGTGAT CAACAGGGAG 
AGGATGGCTT CCTTTGAGCG GTTACTGTGG CGAATCTGCC GAGGAAACGT GTACTTGAAG 
TTCAGTGAGA TGGACGCCCC TCTGGAGGAT CCTGTGACGA AAGAAGAAAT TCAGAAGAAC 
ATATTCATCA TATTTTACCA AGGAGAGCAG CTCAGGCAGA AAATCAAGAA GATCTGTGAT 
GGGTTTCGAG CCACTGTCTA CCCTTGCCCA GAGCCTGCGG TGGAGCGCAG AGAGATGTTG 
GAGAGCGTCA ATGTGAGGCT GGAAGATTTA ATCACCGTCA TAACACAAAC AGAGTCTCAC 
CGCCAGCGCC TGCTGCAGGA AGCCGCTGCC AACTGGCACT CCTGGCTCAT CAAGGTGCAG 
AAGATGAAAG CTGTCTACCA CATCCTGAAC ATGTGCAACA TCGACGTCAC CCAGCAGTGT 
GTCATCGCCG AGATCTGGTT CCCGGTGGCA GATGCCACAC GTATCAAGAG GGCACTGGAG 
CAAGGCATGG AACTAAGTGG CTCCTCCATG GCCCCCATCA TGACCACAGT GCAATCTAAA 
ACAGCCCCTC CCACATTTAA CAGGACCAAT AAATTCACAG CTGGCTTCCA GAATATTGTT 
GATGCCTATG GTGTCGGCAG CTACCGGGAG ATAAACCCAG CCCCCTACAC CATCATCACT 
TTCCCCTTCC TGTTCGCTGT GATGTTTGGA GACTGTGGTC ATGGAACCGT GATGCTCCTG 
GCTGCACTTT GGATGATTCT GAATGAGAGA CGCTTGCTCT CCCAGAAGAC AGACAATGAG 
ATTTGGAACA CCTTCTTCCA CGGGCGCTAT CTGATCCTAC TTATGGGCAT CTTCTCCATC 
TACACGGGTT TGATCTACAA TGACTGCTTC TCCAAGTCCT TGAACATCTT TGGCTCTTCT 
TGGAGTGTCC AACCCATGTT CAGAAACGGC ACATGGAATA CTCATGTAAT GGAGGAAAGT 
CTATATCTGC AGCTGGACCC AGCCATACCA GGAGTGTATT TTGGAAATCC ATACCCGTTT 
GGGATTGATC CGATTTGGAA CTTGGCTTCA AACAAACTCA CATTTCTGAA CTCGTATAAA 
ATGAAGATGT CGGTGATCCT GGGAATTGTC CAGATGGTTT TCGGTGTCAT CCTCAGCCTT 
TTCAATCACA TATACTTCAG AAGAACTCTC AACATCATTC TGCAATTTAT CCCTGAGATG 
ATTTTTATCC TGTGTCTGTT TGGATACCTG GTTTTCATGA TCATTTTCAA ATGGTGCTGC 
TTTGACGTCC ATGTATCTCA GCACGCCCCC AGCATCCTCA TCCACTTCAT CAACATGTTT 
CTGTTTAACT ACAGTGACTC TTCCAACGCA CCCCTCTACA AACATCAGCA AGAAGTCCAA 
AGTTTCTTTG TGGTTATGGC TTTGATTTCT GTGCCGTGGA TGCTTCTGAT TAAGCCGTTT 
ATTCTTAGAG CCAGTCATCG GAAATCCCAG CTGCAGGCAT CCAGGATCCA AGAAGATGCC 
ACTGAGAACA TTGAAGGTGA TAGCTCCAGC CCTTCTAGCC GTTCTGGCCA GAGGACTTCT 
GCAGATACCC ACGGGGCTCT GGACGACCAT GGAGAAGAGT TCAACTTTGG AGACGTCTTT 
GTCCACCAAG CCATCCACAC CATCGAGTAC TGCCTGGGCT GCATTTCAAA CACAGCCTCC 
TACCTGCGGC TCTGGGCCCT CAGCCTGGCT CATGCACAAC TGTCTGAAGT GCTCTGGACT 
ATGGTGATGA ACAGCGGCCT TCAGACGCGA GGCTGGGGAG GAATCGTCGG GGTTTTTATT 
ATTTTTGCCG TATTTGCTGT CCTGACAGTA GCCATCCTTC TGATCATGGA GGGCCTCTCT 
GCTTTCCTGC ACGCCCTGCG ACTGCACTGG GTTGAGTTCC AGAACAAGTT CTATGTCGGG 
GATGGTTACA AGTTTTCTCC ATTCTCCTTT AAACACATCC TGGATGGCAC AGCCGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570855.2
CDS	181..2703
Translation	MVSVFRSEEMCLSQLFLQVEAAYCCVAELGELGLVQFKDLNMNVNSFQRKFVNEVRRCESLERILRFLEDEMQNEIVVQLLEKSPLTPLPREMITLETVLEKLEGELQEANQNQQALKQSFLELTELKYLLKKTQDFFETETNLADDFFTEDTSGLLELKAVPAYMTGKLGFIAGVINRERMASFERLLWRICRGNVYLKFSEMDAPLEDPVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPEPAVERREMLESVNVRLEDLITVITQTESHRQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAEIWFPVADATRIKRALEQGMELSGSSMAPIMTTVQSKTAPPTFNRTNKFTAGFQNIVDAYGVGSYREINPAPYTIITFPFLFAVMFGDCGHGTVMLLAALWMILNERRLLSQKTDNEIWNTFFHGRYLILLMGIFSIYTGLIYNDCFSKSLNIFGSSWSVQPMFRNGTWNTHVMEESLYLQLDPAIPGVYFGNPYPFGIDPIWNLASNKLTFLNSYKMKMSVILGIVQMVFGVILSLFNHIYFRRTLNIILQFIPEMIFILCLFGYLVFMIIFKWCCFDVHVSQHAPSILIHFINMFLFNYSDSSNAPLYKHQQEVQSFFVVMALISVPWMLLIKPFILRASHRKSQLQASRIQEDATENIEGDSSSPSSRSGQRTSADTHGALDDHGEEFNFGDVFVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVMNSGLQTRGWGGIVGVFIIFAVFAVLTVAILLIMEGLSAFLHALRLHWVEFQNKFYVGDGYKFSPFSFKHILDGTAEE

Target ORF information:

RefSeq Version	NM_130840.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130840.3

ORF Insert Sequence:

ATGGTGTCTG TGTTTCGAAG CGAGGAGATG TGTTTGTCAC AACTGTTTCT CCAGGTGGAA 
GCTGCATATT GCTGTGTGGC TGAGCTCGGA GAGCTCGGAT TGGTTCAGTT CAAAGATTTA 
AATATGAATG TGAACAGCTT TCAAAGGAAA TTTGTGAATG AAGTCAGAAG GTGTGAATCA 
CTGGAGAGAA TCCTCCGTTT TCTGGAAGAC GAGATGCAAA ATGAGATTGT AGTTCAGTTG 
CTCGAGAAAA GCCCACTGAC CCCGCTCCCA CGGGAAATGA TTACCCTGGA GACTGTTCTA 
GAAAAACTGG AAGGAGAGTT ACAGGAAGCC AACCAGAACC AGCAGGCCTT GAAACAAAGC 
TTCCTAGAAC TGACAGAACT GAAATACCTC CTGAAGAAAA CCCAAGACTT CTTTGAGACG 
GAAACCAATT TAGCTGATGA TTTCTTTACT GAGGACACTT CTGGCCTCCT GGAGTTGAAA 
GCAGTGCCTG CATATATGAC CGGAAAGTTG GGGTTCATAG CCGGTGTGAT CAACAGGGAG 
AGGATGGCTT CCTTTGAGCG GTTACTGTGG CGAATCTGCC GAGGAAACGT GTACTTGAAG 
TTCAGTGAGA TGGACGCCCC TCTGGAGGAT CCTGTGACGA AAGAAGAAAT TCAGAAGAAC 
ATATTCATCA TATTTTACCA AGGAGAGCAG CTCAGGCAGA AAATCAAGAA GATCTGTGAT 
GGGTTTCGAG CCACTGTCTA CCCTTGCCCA GAGCCTGCGG TGGAGCGCAG AGAGATGTTG 
GAGAGCGTCA ATGTGAGGCT GGAAGATTTA ATCACCGTCA TAACACAAAC AGAGTCTCAC 
CGCCAGCGCC TGCTGCAGGA AGCCGCTGCC AACTGGCACT CCTGGCTCAT CAAGGTGCAG 
AAGATGAAAG CTGTCTACCA CATCCTGAAC ATGTGCAACA TCGACGTCAC CCAGCAGTGT 
GTCATCGCCG AGATCTGGTT CCCGGTGGCA GATGCCACAC GTATCAAGAG GGCACTGGAG 
CAAGGCATGG AACTAAGTGG CTCCTCCATG GCCCCCATCA TGACCACAGT GCAATCTAAA 
ACAGCCCCTC CCACATTTAA CAGGACCAAT AAATTCACAG CTGGCTTCCA GAATATTGTT 
GATGCCTATG GTGTCGGCAG CTACCGGGAG ATAAACCCAG CCCCCTACAC CATCATCACT 
TTCCCCTTCC TGTTCGCTGT GATGTTTGGA GACTGTGGTC ATGGAACCGT GATGCTCCTG 
GCTGCACTTT GGATGATTCT GAATGAGAGA CGCTTGCTCT CCCAGAAGAC AGACAATGAG 
ATTTGGAACA CCTTCTTCCA CGGGCGCTAT CTGATCCTAC TTATGGGCAT CTTCTCCATC 
TACACGGGTT TGATCTACAA TGACTGCTTC TCCAAGTCCT TGAACATCTT TGGCTCTTCT 
TGGAGTGTCC AACCCATGTT CAGAAACGGC ACATGGAATA CTCATGTAAT GGAGGAAAGT 
CTATATCTGC AGCTGGACCC AGCCATACCA GGAGTGTATT TTGGAAATCC ATACCCGTTT 
GGGATTGATC CGATTTGGAA CTTGGCTTCA AACAAACTCA CATTTCTGAA CTCGTATAAA 
ATGAAGATGT CGGTGATCCT GGGAATTGTC CAGATGGTTT TCGGTGTCAT CCTCAGCCTT 
TTCAATCACA TATACTTCAG AAGAACTCTC AACATCATTC TGCAATTTAT CCCTGAGATG 
ATTTTTATCC TGTGTCTGTT TGGATACCTG GTTTTCATGA TCATTTTCAA ATGGTGCTGC 
TTTGACGTCC ATGTATCTCA GCACGCCCCC AGCATCCTCA TCCACTTCAT CAACATGTTT 
CTGTTTAACT ACAGTGACTC TTCCAACGCA CCCCTCTACA AACATCAGCA AGAAGTCCAA 
AGTTTCTTTG TGGTTATGGC TTTGATTTCT GTGCCGTGGA TGCTTCTGAT TAAGCCGTTT 
ATTCTTAGAG CCAGTCATCG GAAATCCCAG CTGCAGGCAT CCAGGATCCA AGAAGATGCC 
ACTGAGAACA TTGAAGGTGA TAGCTCCAGC CCTTCTAGCC GTTCTGGCCA GAGGACTTCT 
GCAGATACCC ACGGGGCTCT GGACGACCAT GGAGAAGAGT TCAACTTTGG AGACGTCTTT 
GTCCACCAAG CCATCCACAC CATCGAGTAC TGCCTGGGCT GCATTTCAAA CACAGCCTCC 
TACCTGCGGC TCTGGGCCCT CAGCCTGGCT CATGCACAAC TGTCTGAAGT GCTCTGGACT 
ATGGTGATGA ACAGCGGCCT TCAGACGCGA GGCTGGGGAG GAATCGTCGG GGTTTTTATT 
ATTTTTGCCG TATTTGCTGT CCTGACAGTA GCCATCCTTC TGATCATGGA GGGCCTCTCT 
GCTTTCCTGC ACGCCCTGCG ACTGCACTGG GTTGAGTTCC AGAACAAGTT CTATGTCGGG 
GATGGTTACA AGTTTTCTCC ATTCTCCTTT AAACACATCC TGGATGGCAC AGCCGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V0A4 ( NM_130840.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_570855.2 Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant 2, mRNA.

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CloneID	OHu15055
Clone ID Related Accession (Same CDS sequence)	XM_005250393.1 , XM_005250394.3 , NM_130841.2 , NM_020632.3 , NM_020632.2 , NM_130840.3 , NM_130840.2
Accession Version	NM_130840.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	V-type proton ATPase 116 kDa subunit a isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB230144.1, BC109305.1, AF245517.1 and BC109304.1. On Jan 19, 2006 this sequence version replaced NM_130840.1. Transcript Variant: This variant (2) lacks an exon in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC109304.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968832, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGTCTG TGTTTCGAAG CGAGGAGATG TGTTTGTCAC AACTGTTTCT CCAGGTGGAA 
GCTGCATATT GCTGTGTGGC TGAGCTCGGA GAGCTCGGAT TGGTTCAGTT CAAAGATTTA 
AATATGAATG TGAACAGCTT TCAAAGGAAA TTTGTGAATG AAGTCAGAAG GTGTGAATCA 
CTGGAGAGAA TCCTCCGTTT TCTGGAAGAC GAGATGCAAA ATGAGATTGT AGTTCAGTTG 
CTCGAGAAAA GCCCACTGAC CCCGCTCCCA CGGGAAATGA TTACCCTGGA GACTGTTCTA 
GAAAAACTGG AAGGAGAGTT ACAGGAAGCC AACCAGAACC AGCAGGCCTT GAAACAAAGC 
TTCCTAGAAC TGACAGAACT GAAATACCTC CTGAAGAAAA CCCAAGACTT CTTTGAGACG 
GAAACCAATT TAGCTGATGA TTTCTTTACT GAGGACACTT CTGGCCTCCT GGAGTTGAAA 
GCAGTGCCTG CATATATGAC CGGAAAGTTG GGGTTCATAG CCGGTGTGAT CAACAGGGAG 
AGGATGGCTT CCTTTGAGCG GTTACTGTGG CGAATCTGCC GAGGAAACGT GTACTTGAAG 
TTCAGTGAGA TGGACGCCCC TCTGGAGGAT CCTGTGACGA AAGAAGAAAT TCAGAAGAAC 
ATATTCATCA TATTTTACCA AGGAGAGCAG CTCAGGCAGA AAATCAAGAA GATCTGTGAT 
GGGTTTCGAG CCACTGTCTA CCCTTGCCCA GAGCCTGCGG TGGAGCGCAG AGAGATGTTG 
GAGAGCGTCA ATGTGAGGCT GGAAGATTTA ATCACCGTCA TAACACAAAC AGAGTCTCAC 
CGCCAGCGCC TGCTGCAGGA AGCCGCTGCC AACTGGCACT CCTGGCTCAT CAAGGTGCAG 
AAGATGAAAG CTGTCTACCA CATCCTGAAC ATGTGCAACA TCGACGTCAC CCAGCAGTGT 
GTCATCGCCG AGATCTGGTT CCCGGTGGCA GATGCCACAC GTATCAAGAG GGCACTGGAG 
CAAGGCATGG AACTAAGTGG CTCCTCCATG GCCCCCATCA TGACCACAGT GCAATCTAAA 
ACAGCCCCTC CCACATTTAA CAGGACCAAT AAATTCACAG CTGGCTTCCA GAATATTGTT 
GATGCCTATG GTGTCGGCAG CTACCGGGAG ATAAACCCAG CCCCCTACAC CATCATCACT 
TTCCCCTTCC TGTTCGCTGT GATGTTTGGA GACTGTGGTC ATGGAACCGT GATGCTCCTG 
GCTGCACTTT GGATGATTCT GAATGAGAGA CGCTTGCTCT CCCAGAAGAC AGACAATGAG 
ATTTGGAACA CCTTCTTCCA CGGGCGCTAT CTGATCCTAC TTATGGGCAT CTTCTCCATC 
TACACGGGTT TGATCTACAA TGACTGCTTC TCCAAGTCCT TGAACATCTT TGGCTCTTCT 
TGGAGTGTCC AACCCATGTT CAGAAACGGC ACATGGAATA CTCATGTAAT GGAGGAAAGT 
CTATATCTGC AGCTGGACCC AGCCATACCA GGAGTGTATT TTGGAAATCC ATACCCGTTT 
GGGATTGATC CGATTTGGAA CTTGGCTTCA AACAAACTCA CATTTCTGAA CTCGTATAAA 
ATGAAGATGT CGGTGATCCT GGGAATTGTC CAGATGGTTT TCGGTGTCAT CCTCAGCCTT 
TTCAATCACA TATACTTCAG AAGAACTCTC AACATCATTC TGCAATTTAT CCCTGAGATG 
ATTTTTATCC TGTGTCTGTT TGGATACCTG GTTTTCATGA TCATTTTCAA ATGGTGCTGC 
TTTGACGTCC ATGTATCTCA GCACGCCCCC AGCATCCTCA TCCACTTCAT CAACATGTTT 
CTGTTTAACT ACAGTGACTC TTCCAACGCA CCCCTCTACA AACATCAGCA AGAAGTCCAA 
AGTTTCTTTG TGGTTATGGC TTTGATTTCT GTGCCGTGGA TGCTTCTGAT TAAGCCGTTT 
ATTCTTAGAG CCAGTCATCG GAAATCCCAG CTGCAGGCAT CCAGGATCCA AGAAGATGCC 
ACTGAGAACA TTGAAGGTGA TAGCTCCAGC CCTTCTAGCC GTTCTGGCCA GAGGACTTCT 
GCAGATACCC ACGGGGCTCT GGACGACCAT GGAGAAGAGT TCAACTTTGG AGACGTCTTT 
GTCCACCAAG CCATCCACAC CATCGAGTAC TGCCTGGGCT GCATTTCAAA CACAGCCTCC 
TACCTGCGGC TCTGGGCCCT CAGCCTGGCT CATGCACAAC TGTCTGAAGT GCTCTGGACT 
ATGGTGATGA ACAGCGGCCT TCAGACGCGA GGCTGGGGAG GAATCGTCGG GGTTTTTATT 
ATTTTTGCCG TATTTGCTGT CCTGACAGTA GCCATCCTTC TGATCATGGA GGGCCTCTCT 
GCTTTCCTGC ACGCCCTGCG ACTGCACTGG GTTGAGTTCC AGAACAAGTT CTATGTCGGG 
GATGGTTACA AGTTTTCTCC ATTCTCCTTT AAACACATCC TGGATGGCAC AGCCGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570855.2
CDS	181..2703
Translation	MVSVFRSEEMCLSQLFLQVEAAYCCVAELGELGLVQFKDLNMNVNSFQRKFVNEVRRCESLERILRFLEDEMQNEIVVQLLEKSPLTPLPREMITLETVLEKLEGELQEANQNQQALKQSFLELTELKYLLKKTQDFFETETNLADDFFTEDTSGLLELKAVPAYMTGKLGFIAGVINRERMASFERLLWRICRGNVYLKFSEMDAPLEDPVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPEPAVERREMLESVNVRLEDLITVITQTESHRQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAEIWFPVADATRIKRALEQGMELSGSSMAPIMTTVQSKTAPPTFNRTNKFTAGFQNIVDAYGVGSYREINPAPYTIITFPFLFAVMFGDCGHGTVMLLAALWMILNERRLLSQKTDNEIWNTFFHGRYLILLMGIFSIYTGLIYNDCFSKSLNIFGSSWSVQPMFRNGTWNTHVMEESLYLQLDPAIPGVYFGNPYPFGIDPIWNLASNKLTFLNSYKMKMSVILGIVQMVFGVILSLFNHIYFRRTLNIILQFIPEMIFILCLFGYLVFMIIFKWCCFDVHVSQHAPSILIHFINMFLFNYSDSSNAPLYKHQQEVQSFFVVMALISVPWMLLIKPFILRASHRKSQLQASRIQEDATENIEGDSSSPSSRSGQRTSADTHGALDDHGEEFNFGDVFVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVMNSGLQTRGWGGIVGVFIIFAVFAVLTVAILLIMEGLSAFLHALRLHWVEFQNKFYVGDGYKFSPFSFKHILDGTAEE

Target ORF information:

RefSeq Version	NM_130840.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130840.2

ORF Insert Sequence:

ATGGTGTCTG TGTTTCGAAG CGAGGAGATG TGTTTGTCAC AACTGTTTCT CCAGGTGGAA 
GCTGCATATT GCTGTGTGGC TGAGCTCGGA GAGCTCGGAT TGGTTCAGTT CAAAGATTTA 
AATATGAATG TGAACAGCTT TCAAAGGAAA TTTGTGAATG AAGTCAGAAG GTGTGAATCA 
CTGGAGAGAA TCCTCCGTTT TCTGGAAGAC GAGATGCAAA ATGAGATTGT AGTTCAGTTG 
CTCGAGAAAA GCCCACTGAC CCCGCTCCCA CGGGAAATGA TTACCCTGGA GACTGTTCTA 
GAAAAACTGG AAGGAGAGTT ACAGGAAGCC AACCAGAACC AGCAGGCCTT GAAACAAAGC 
TTCCTAGAAC TGACAGAACT GAAATACCTC CTGAAGAAAA CCCAAGACTT CTTTGAGACG 
GAAACCAATT TAGCTGATGA TTTCTTTACT GAGGACACTT CTGGCCTCCT GGAGTTGAAA 
GCAGTGCCTG CATATATGAC CGGAAAGTTG GGGTTCATAG CCGGTGTGAT CAACAGGGAG 
AGGATGGCTT CCTTTGAGCG GTTACTGTGG CGAATCTGCC GAGGAAACGT GTACTTGAAG 
TTCAGTGAGA TGGACGCCCC TCTGGAGGAT CCTGTGACGA AAGAAGAAAT TCAGAAGAAC 
ATATTCATCA TATTTTACCA AGGAGAGCAG CTCAGGCAGA AAATCAAGAA GATCTGTGAT 
GGGTTTCGAG CCACTGTCTA CCCTTGCCCA GAGCCTGCGG TGGAGCGCAG AGAGATGTTG 
GAGAGCGTCA ATGTGAGGCT GGAAGATTTA ATCACCGTCA TAACACAAAC AGAGTCTCAC 
CGCCAGCGCC TGCTGCAGGA AGCCGCTGCC AACTGGCACT CCTGGCTCAT CAAGGTGCAG 
AAGATGAAAG CTGTCTACCA CATCCTGAAC ATGTGCAACA TCGACGTCAC CCAGCAGTGT 
GTCATCGCCG AGATCTGGTT CCCGGTGGCA GATGCCACAC GTATCAAGAG GGCACTGGAG 
CAAGGCATGG AACTAAGTGG CTCCTCCATG GCCCCCATCA TGACCACAGT GCAATCTAAA 
ACAGCCCCTC CCACATTTAA CAGGACCAAT AAATTCACAG CTGGCTTCCA GAATATTGTT 
GATGCCTATG GTGTCGGCAG CTACCGGGAG ATAAACCCAG CCCCCTACAC CATCATCACT 
TTCCCCTTCC TGTTCGCTGT GATGTTTGGA GACTGTGGTC ATGGAACCGT GATGCTCCTG 
GCTGCACTTT GGATGATTCT GAATGAGAGA CGCTTGCTCT CCCAGAAGAC AGACAATGAG 
ATTTGGAACA CCTTCTTCCA CGGGCGCTAT CTGATCCTAC TTATGGGCAT CTTCTCCATC 
TACACGGGTT TGATCTACAA TGACTGCTTC TCCAAGTCCT TGAACATCTT TGGCTCTTCT 
TGGAGTGTCC AACCCATGTT CAGAAACGGC ACATGGAATA CTCATGTAAT GGAGGAAAGT 
CTATATCTGC AGCTGGACCC AGCCATACCA GGAGTGTATT TTGGAAATCC ATACCCGTTT 
GGGATTGATC CGATTTGGAA CTTGGCTTCA AACAAACTCA CATTTCTGAA CTCGTATAAA 
ATGAAGATGT CGGTGATCCT GGGAATTGTC CAGATGGTTT TCGGTGTCAT CCTCAGCCTT 
TTCAATCACA TATACTTCAG AAGAACTCTC AACATCATTC TGCAATTTAT CCCTGAGATG 
ATTTTTATCC TGTGTCTGTT TGGATACCTG GTTTTCATGA TCATTTTCAA ATGGTGCTGC 
TTTGACGTCC ATGTATCTCA GCACGCCCCC AGCATCCTCA TCCACTTCAT CAACATGTTT 
CTGTTTAACT ACAGTGACTC TTCCAACGCA CCCCTCTACA AACATCAGCA AGAAGTCCAA 
AGTTTCTTTG TGGTTATGGC TTTGATTTCT GTGCCGTGGA TGCTTCTGAT TAAGCCGTTT 
ATTCTTAGAG CCAGTCATCG GAAATCCCAG CTGCAGGCAT CCAGGATCCA AGAAGATGCC 
ACTGAGAACA TTGAAGGTGA TAGCTCCAGC CCTTCTAGCC GTTCTGGCCA GAGGACTTCT 
GCAGATACCC ACGGGGCTCT GGACGACCAT GGAGAAGAGT TCAACTTTGG AGACGTCTTT 
GTCCACCAAG CCATCCACAC CATCGAGTAC TGCCTGGGCT GCATTTCAAA CACAGCCTCC 
TACCTGCGGC TCTGGGCCCT CAGCCTGGCT CATGCACAAC TGTCTGAAGT GCTCTGGACT 
ATGGTGATGA ACAGCGGCCT TCAGACGCGA GGCTGGGGAG GAATCGTCGG GGTTTTTATT 
ATTTTTGCCG TATTTGCTGT CCTGACAGTA GCCATCCTTC TGATCATGGA GGGCCTCTCT 
GCTTTCCTGC ACGCCCTGCG ACTGCACTGG GTTGAGTTCC AGAACAAGTT CTATGTCGGG 
GATGGTTACA AGTTTTCTCC ATTCTCCTTT AAACACATCC TGGATGGCAC AGCCGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V0A4 ( XM_005250393.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005250450.1 Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu15055
Clone ID Related Accession (Same CDS sequence)	XM_005250393.1 , XM_005250394.3 , NM_130841.2 , NM_020632.3 , NM_020632.2 , NM_130840.3 , NM_130840.2
Accession Version	XM_005250393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	V-type proton ATPase 116 kDa subunit a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTCTG TGTTTCGAAG CGAGGAGATG TGTTTGTCAC AACTGTTTCT CCAGGTGGAA 
GCTGCATATT GCTGTGTGGC TGAGCTCGGA GAGCTCGGAT TGGTTCAGTT CAAAGATTTA 
AATATGAATG TGAACAGCTT TCAAAGGAAA TTTGTGAATG AAGTCAGAAG GTGTGAATCA 
CTGGAGAGAA TCCTCCGTTT TCTGGAAGAC GAGATGCAAA ATGAGATTGT AGTTCAGTTG 
CTCGAGAAAA GCCCACTGAC CCCGCTCCCA CGGGAAATGA TTACCCTGGA GACTGTTCTA 
GAAAAACTGG AAGGAGAGTT ACAGGAAGCC AACCAGAACC AGCAGGCCTT GAAACAAAGC 
TTCCTAGAAC TGACAGAACT GAAATACCTC CTGAAGAAAA CCCAAGACTT CTTTGAGACG 
GAAACCAATT TAGCTGATGA TTTCTTTACT GAGGACACTT CTGGCCTCCT GGAGTTGAAA 
GCAGTGCCTG CATATATGAC CGGAAAGTTG GGGTTCATAG CCGGTGTGAT CAACAGGGAG 
AGGATGGCTT CCTTTGAGCG GTTACTGTGG CGAATCTGCC GAGGAAACGT GTACTTGAAG 
TTCAGTGAGA TGGACGCCCC TCTGGAGGAT CCTGTGACGA AAGAAGAAAT TCAGAAGAAC 
ATATTCATCA TATTTTACCA AGGAGAGCAG CTCAGGCAGA AAATCAAGAA GATCTGTGAT 
GGGTTTCGAG CCACTGTCTA CCCTTGCCCA GAGCCTGCGG TGGAGCGCAG AGAGATGTTG 
GAGAGCGTCA ATGTGAGGCT GGAAGATTTA ATCACCGTCA TAACACAAAC AGAGTCTCAC 
CGCCAGCGCC TGCTGCAGGA AGCCGCTGCC AACTGGCACT CCTGGCTCAT CAAGGTGCAG 
AAGATGAAAG CTGTCTACCA CATCCTGAAC ATGTGCAACA TCGACGTCAC CCAGCAGTGT 
GTCATCGCCG AGATCTGGTT CCCGGTGGCA GATGCCACAC GTATCAAGAG GGCACTGGAG 
CAAGGCATGG AACTAAGTGG CTCCTCCATG GCCCCCATCA TGACCACAGT GCAATCTAAA 
ACAGCCCCTC CCACATTTAA CAGGACCAAT AAATTCACAG CTGGCTTCCA GAATATTGTT 
GATGCCTATG GTGTCGGCAG CTACCGGGAG ATAAACCCAG CCCCCTACAC CATCATCACT 
TTCCCCTTCC TGTTCGCTGT GATGTTTGGA GACTGTGGTC ATGGAACCGT GATGCTCCTG 
GCTGCACTTT GGATGATTCT GAATGAGAGA CGCTTGCTCT CCCAGAAGAC AGACAATGAG 
ATTTGGAACA CCTTCTTCCA CGGGCGCTAT CTGATCCTAC TTATGGGCAT CTTCTCCATC 
TACACGGGTT TGATCTACAA TGACTGCTTC TCCAAGTCCT TGAACATCTT TGGCTCTTCT 
TGGAGTGTCC AACCCATGTT CAGAAACGGC ACATGGAATA CTCATGTAAT GGAGGAAAGT 
CTATATCTGC AGCTGGACCC AGCCATACCA GGAGTGTATT TTGGAAATCC ATACCCGTTT 
GGGATTGATC CGATTTGGAA CTTGGCTTCA AACAAACTCA CATTTCTGAA CTCGTATAAA 
ATGAAGATGT CGGTGATCCT GGGAATTGTC CAGATGGTTT TCGGTGTCAT CCTCAGCCTT 
TTCAATCACA TATACTTCAG AAGAACTCTC AACATCATTC TGCAATTTAT CCCTGAGATG 
ATTTTTATCC TGTGTCTGTT TGGATACCTG GTTTTCATGA TCATTTTCAA ATGGTGCTGC 
TTTGACGTCC ATGTATCTCA GCACGCCCCC AGCATCCTCA TCCACTTCAT CAACATGTTT 
CTGTTTAACT ACAGTGACTC TTCCAACGCA CCCCTCTACA AACATCAGCA AGAAGTCCAA 
AGTTTCTTTG TGGTTATGGC TTTGATTTCT GTGCCGTGGA TGCTTCTGAT TAAGCCGTTT 
ATTCTTAGAG CCAGTCATCG GAAATCCCAG CTGCAGGCAT CCAGGATCCA AGAAGATGCC 
ACTGAGAACA TTGAAGGTGA TAGCTCCAGC CCTTCTAGCC GTTCTGGCCA GAGGACTTCT 
GCAGATACCC ACGGGGCTCT GGACGACCAT GGAGAAGAGT TCAACTTTGG AGACGTCTTT 
GTCCACCAAG CCATCCACAC CATCGAGTAC TGCCTGGGCT GCATTTCAAA CACAGCCTCC 
TACCTGCGGC TCTGGGCCCT CAGCCTGGCT CATGCACAAC TGTCTGAAGT GCTCTGGACT 
ATGGTGATGA ACAGCGGCCT TCAGACGCGA GGCTGGGGAG GAATCGTCGG GGTTTTTATT 
ATTTTTGCCG TATTTGCTGT CCTGACAGTA GCCATCCTTC TGATCATGGA GGGCCTCTCT 
GCTTTCCTGC ACGCCCTGCG ACTGCACTGG GTTGAGTTCC AGAACAAGTT CTATGTCGGG 
GATGGTTACA AGTTTTCTCC ATTCTCCTTT AAACACATCC TGGATGGCAC AGCCGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005250450.1
CDS	631..3153
Translation	MVSVFRSEEMCLSQLFLQVEAAYCCVAELGELGLVQFKDLNMNVNSFQRKFVNEVRRCESLERILRFLEDEMQNEIVVQLLEKSPLTPLPREMITLETVLEKLEGELQEANQNQQALKQSFLELTELKYLLKKTQDFFETETNLADDFFTEDTSGLLELKAVPAYMTGKLGFIAGVINRERMASFERLLWRICRGNVYLKFSEMDAPLEDPVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPEPAVERREMLESVNVRLEDLITVITQTESHRQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAEIWFPVADATRIKRALEQGMELSGSSMAPIMTTVQSKTAPPTFNRTNKFTAGFQNIVDAYGVGSYREINPAPYTIITFPFLFAVMFGDCGHGTVMLLAALWMILNERRLLSQKTDNEIWNTFFHGRYLILLMGIFSIYTGLIYNDCFSKSLNIFGSSWSVQPMFRNGTWNTHVMEESLYLQLDPAIPGVYFGNPYPFGIDPIWNLASNKLTFLNSYKMKMSVILGIVQMVFGVILSLFNHIYFRRTLNIILQFIPEMIFILCLFGYLVFMIIFKWCCFDVHVSQHAPSILIHFINMFLFNYSDSSNAPLYKHQQEVQSFFVVMALISVPWMLLIKPFILRASHRKSQLQASRIQEDATENIEGDSSSPSSRSGQRTSADTHGALDDHGEEFNFGDVFVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVMNSGLQTRGWGGIVGVFIIFAVFAVLTVAILLIMEGLSAFLHALRLHWVEFQNKFYVGDGYKFSPFSFKHILDGTAEE

Target ORF information:

RefSeq Version	XM_005250393.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005250393.1

ORF Insert Sequence:

ATGGTGTCTG TGTTTCGAAG CGAGGAGATG TGTTTGTCAC AACTGTTTCT CCAGGTGGAA 
GCTGCATATT GCTGTGTGGC TGAGCTCGGA GAGCTCGGAT TGGTTCAGTT CAAAGATTTA 
AATATGAATG TGAACAGCTT TCAAAGGAAA TTTGTGAATG AAGTCAGAAG GTGTGAATCA 
CTGGAGAGAA TCCTCCGTTT TCTGGAAGAC GAGATGCAAA ATGAGATTGT AGTTCAGTTG 
CTCGAGAAAA GCCCACTGAC CCCGCTCCCA CGGGAAATGA TTACCCTGGA GACTGTTCTA 
GAAAAACTGG AAGGAGAGTT ACAGGAAGCC AACCAGAACC AGCAGGCCTT GAAACAAAGC 
TTCCTAGAAC TGACAGAACT GAAATACCTC CTGAAGAAAA CCCAAGACTT CTTTGAGACG 
GAAACCAATT TAGCTGATGA TTTCTTTACT GAGGACACTT CTGGCCTCCT GGAGTTGAAA 
GCAGTGCCTG CATATATGAC CGGAAAGTTG GGGTTCATAG CCGGTGTGAT CAACAGGGAG 
AGGATGGCTT CCTTTGAGCG GTTACTGTGG CGAATCTGCC GAGGAAACGT GTACTTGAAG 
TTCAGTGAGA TGGACGCCCC TCTGGAGGAT CCTGTGACGA AAGAAGAAAT TCAGAAGAAC 
ATATTCATCA TATTTTACCA AGGAGAGCAG CTCAGGCAGA AAATCAAGAA GATCTGTGAT 
GGGTTTCGAG CCACTGTCTA CCCTTGCCCA GAGCCTGCGG TGGAGCGCAG AGAGATGTTG 
GAGAGCGTCA ATGTGAGGCT GGAAGATTTA ATCACCGTCA TAACACAAAC AGAGTCTCAC 
CGCCAGCGCC TGCTGCAGGA AGCCGCTGCC AACTGGCACT CCTGGCTCAT CAAGGTGCAG 
AAGATGAAAG CTGTCTACCA CATCCTGAAC ATGTGCAACA TCGACGTCAC CCAGCAGTGT 
GTCATCGCCG AGATCTGGTT CCCGGTGGCA GATGCCACAC GTATCAAGAG GGCACTGGAG 
CAAGGCATGG AACTAAGTGG CTCCTCCATG GCCCCCATCA TGACCACAGT GCAATCTAAA 
ACAGCCCCTC CCACATTTAA CAGGACCAAT AAATTCACAG CTGGCTTCCA GAATATTGTT 
GATGCCTATG GTGTCGGCAG CTACCGGGAG ATAAACCCAG CCCCCTACAC CATCATCACT 
TTCCCCTTCC TGTTCGCTGT GATGTTTGGA GACTGTGGTC ATGGAACCGT GATGCTCCTG 
GCTGCACTTT GGATGATTCT GAATGAGAGA CGCTTGCTCT CCCAGAAGAC AGACAATGAG 
ATTTGGAACA CCTTCTTCCA CGGGCGCTAT CTGATCCTAC TTATGGGCAT CTTCTCCATC 
TACACGGGTT TGATCTACAA TGACTGCTTC TCCAAGTCCT TGAACATCTT TGGCTCTTCT 
TGGAGTGTCC AACCCATGTT CAGAAACGGC ACATGGAATA CTCATGTAAT GGAGGAAAGT 
CTATATCTGC AGCTGGACCC AGCCATACCA GGAGTGTATT TTGGAAATCC ATACCCGTTT 
GGGATTGATC CGATTTGGAA CTTGGCTTCA AACAAACTCA CATTTCTGAA CTCGTATAAA 
ATGAAGATGT CGGTGATCCT GGGAATTGTC CAGATGGTTT TCGGTGTCAT CCTCAGCCTT 
TTCAATCACA TATACTTCAG AAGAACTCTC AACATCATTC TGCAATTTAT CCCTGAGATG 
ATTTTTATCC TGTGTCTGTT TGGATACCTG GTTTTCATGA TCATTTTCAA ATGGTGCTGC 
TTTGACGTCC ATGTATCTCA GCACGCCCCC AGCATCCTCA TCCACTTCAT CAACATGTTT 
CTGTTTAACT ACAGTGACTC TTCCAACGCA CCCCTCTACA AACATCAGCA AGAAGTCCAA 
AGTTTCTTTG TGGTTATGGC TTTGATTTCT GTGCCGTGGA TGCTTCTGAT TAAGCCGTTT 
ATTCTTAGAG CCAGTCATCG GAAATCCCAG CTGCAGGCAT CCAGGATCCA AGAAGATGCC 
ACTGAGAACA TTGAAGGTGA TAGCTCCAGC CCTTCTAGCC GTTCTGGCCA GAGGACTTCT 
GCAGATACCC ACGGGGCTCT GGACGACCAT GGAGAAGAGT TCAACTTTGG AGACGTCTTT 
GTCCACCAAG CCATCCACAC CATCGAGTAC TGCCTGGGCT GCATTTCAAA CACAGCCTCC 
TACCTGCGGC TCTGGGCCCT CAGCCTGGCT CATGCACAAC TGTCTGAAGT GCTCTGGACT 
ATGGTGATGA ACAGCGGCCT TCAGACGCGA GGCTGGGGAG GAATCGTCGG GGTTTTTATT 
ATTTTTGCCG TATTTGCTGT CCTGACAGTA GCCATCCTTC TGATCATGGA GGGCCTCTCT 
GCTTTCCTGC ACGCCCTGCG ACTGCACTGG GTTGAGTTCC AGAACAAGTT CTATGTCGGG 
GATGGTTACA AGTTTTCTCC ATTCTCCTTT AAACACATCC TGGATGGCAC AGCCGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V0A4 ( XM_005250394.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005250451.1 Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant X2, mRNA.

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CloneID	OHu15055
Clone ID Related Accession (Same CDS sequence)	XM_005250393.1 , XM_005250394.3 , NM_130841.2 , NM_020632.3 , NM_020632.2 , NM_130840.3 , NM_130840.2
Accession Version	XM_005250394.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	V-type proton ATPase 116 kDa subunit a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005250394.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTCTG TGTTTCGAAG CGAGGAGATG TGTTTGTCAC AACTGTTTCT CCAGGTGGAA 
GCTGCATATT GCTGTGTGGC TGAGCTCGGA GAGCTCGGAT TGGTTCAGTT CAAAGATTTA 
AATATGAATG TGAACAGCTT TCAAAGGAAA TTTGTGAATG AAGTCAGAAG GTGTGAATCA 
CTGGAGAGAA TCCTCCGTTT TCTGGAAGAC GAGATGCAAA ATGAGATTGT AGTTCAGTTG 
CTCGAGAAAA GCCCACTGAC CCCGCTCCCA CGGGAAATGA TTACCCTGGA GACTGTTCTA 
GAAAAACTGG AAGGAGAGTT ACAGGAAGCC AACCAGAACC AGCAGGCCTT GAAACAAAGC 
TTCCTAGAAC TGACAGAACT GAAATACCTC CTGAAGAAAA CCCAAGACTT CTTTGAGACG 
GAAACCAATT TAGCTGATGA TTTCTTTACT GAGGACACTT CTGGCCTCCT GGAGTTGAAA 
GCAGTGCCTG CATATATGAC CGGAAAGTTG GGGTTCATAG CCGGTGTGAT CAACAGGGAG 
AGGATGGCTT CCTTTGAGCG GTTACTGTGG CGAATCTGCC GAGGAAACGT GTACTTGAAG 
TTCAGTGAGA TGGACGCCCC TCTGGAGGAT CCTGTGACGA AAGAAGAAAT TCAGAAGAAC 
ATATTCATCA TATTTTACCA AGGAGAGCAG CTCAGGCAGA AAATCAAGAA GATCTGTGAT 
GGGTTTCGAG CCACTGTCTA CCCTTGCCCA GAGCCTGCGG TGGAGCGCAG AGAGATGTTG 
GAGAGCGTCA ATGTGAGGCT GGAAGATTTA ATCACCGTCA TAACACAAAC AGAGTCTCAC 
CGCCAGCGCC TGCTGCAGGA AGCCGCTGCC AACTGGCACT CCTGGCTCAT CAAGGTGCAG 
AAGATGAAAG CTGTCTACCA CATCCTGAAC ATGTGCAACA TCGACGTCAC CCAGCAGTGT 
GTCATCGCCG AGATCTGGTT CCCGGTGGCA GATGCCACAC GTATCAAGAG GGCACTGGAG 
CAAGGCATGG AACTAAGTGG CTCCTCCATG GCCCCCATCA TGACCACAGT GCAATCTAAA 
ACAGCCCCTC CCACATTTAA CAGGACCAAT AAATTCACAG CTGGCTTCCA GAATATTGTT 
GATGCCTATG GTGTCGGCAG CTACCGGGAG ATAAACCCAG CCCCCTACAC CATCATCACT 
TTCCCCTTCC TGTTCGCTGT GATGTTTGGA GACTGTGGTC ATGGAACCGT GATGCTCCTG 
GCTGCACTTT GGATGATTCT GAATGAGAGA CGCTTGCTCT CCCAGAAGAC AGACAATGAG 
ATTTGGAACA CCTTCTTCCA CGGGCGCTAT CTGATCCTAC TTATGGGCAT CTTCTCCATC 
TACACGGGTT TGATCTACAA TGACTGCTTC TCCAAGTCCT TGAACATCTT TGGCTCTTCT 
TGGAGTGTCC AACCCATGTT CAGAAACGGC ACATGGAATA CTCATGTAAT GGAGGAAAGT 
CTATATCTGC AGCTGGACCC AGCCATACCA GGAGTGTATT TTGGAAATCC ATACCCGTTT 
GGGATTGATC CGATTTGGAA CTTGGCTTCA AACAAACTCA CATTTCTGAA CTCGTATAAA 
ATGAAGATGT CGGTGATCCT GGGAATTGTC CAGATGGTTT TCGGTGTCAT CCTCAGCCTT 
TTCAATCACA TATACTTCAG AAGAACTCTC AACATCATTC TGCAATTTAT CCCTGAGATG 
ATTTTTATCC TGTGTCTGTT TGGATACCTG GTTTTCATGA TCATTTTCAA ATGGTGCTGC 
TTTGACGTCC ATGTATCTCA GCACGCCCCC AGCATCCTCA TCCACTTCAT CAACATGTTT 
CTGTTTAACT ACAGTGACTC TTCCAACGCA CCCCTCTACA AACATCAGCA AGAAGTCCAA 
AGTTTCTTTG TGGTTATGGC TTTGATTTCT GTGCCGTGGA TGCTTCTGAT TAAGCCGTTT 
ATTCTTAGAG CCAGTCATCG GAAATCCCAG CTGCAGGCAT CCAGGATCCA AGAAGATGCC 
ACTGAGAACA TTGAAGGTGA TAGCTCCAGC CCTTCTAGCC GTTCTGGCCA GAGGACTTCT 
GCAGATACCC ACGGGGCTCT GGACGACCAT GGAGAAGAGT TCAACTTTGG AGACGTCTTT 
GTCCACCAAG CCATCCACAC CATCGAGTAC TGCCTGGGCT GCATTTCAAA CACAGCCTCC 
TACCTGCGGC TCTGGGCCCT CAGCCTGGCT CATGCACAAC TGTCTGAAGT GCTCTGGACT 
ATGGTGATGA ACAGCGGCCT TCAGACGCGA GGCTGGGGAG GAATCGTCGG GGTTTTTATT 
ATTTTTGCCG TATTTGCTGT CCTGACAGTA GCCATCCTTC TGATCATGGA GGGCCTCTCT 
GCTTTCCTGC ACGCCCTGCG ACTGCACTGG GTTGAGTTCC AGAACAAGTT CTATGTCGGG 
GATGGTTACA AGTTTTCTCC ATTCTCCTTT AAACACATCC TGGATGGCAC AGCCGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005250451.1
CDS	543..3065
Translation	MVSVFRSEEMCLSQLFLQVEAAYCCVAELGELGLVQFKDLNMNVNSFQRKFVNEVRRCESLERILRFLEDEMQNEIVVQLLEKSPLTPLPREMITLETVLEKLEGELQEANQNQQALKQSFLELTELKYLLKKTQDFFETETNLADDFFTEDTSGLLELKAVPAYMTGKLGFIAGVINRERMASFERLLWRICRGNVYLKFSEMDAPLEDPVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPEPAVERREMLESVNVRLEDLITVITQTESHRQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAEIWFPVADATRIKRALEQGMELSGSSMAPIMTTVQSKTAPPTFNRTNKFTAGFQNIVDAYGVGSYREINPAPYTIITFPFLFAVMFGDCGHGTVMLLAALWMILNERRLLSQKTDNEIWNTFFHGRYLILLMGIFSIYTGLIYNDCFSKSLNIFGSSWSVQPMFRNGTWNTHVMEESLYLQLDPAIPGVYFGNPYPFGIDPIWNLASNKLTFLNSYKMKMSVILGIVQMVFGVILSLFNHIYFRRTLNIILQFIPEMIFILCLFGYLVFMIIFKWCCFDVHVSQHAPSILIHFINMFLFNYSDSSNAPLYKHQQEVQSFFVVMALISVPWMLLIKPFILRASHRKSQLQASRIQEDATENIEGDSSSPSSRSGQRTSADTHGALDDHGEEFNFGDVFVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVMNSGLQTRGWGGIVGVFIIFAVFAVLTVAILLIMEGLSAFLHALRLHWVEFQNKFYVGDGYKFSPFSFKHILDGTAEE

Target ORF information:

RefSeq Version	XM_005250394.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V0 subunit a4 (ATP6V0A4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005250394.3

ORF Insert Sequence:

ATGGTGTCTG TGTTTCGAAG CGAGGAGATG TGTTTGTCAC AACTGTTTCT CCAGGTGGAA 
GCTGCATATT GCTGTGTGGC TGAGCTCGGA GAGCTCGGAT TGGTTCAGTT CAAAGATTTA 
AATATGAATG TGAACAGCTT TCAAAGGAAA TTTGTGAATG AAGTCAGAAG GTGTGAATCA 
CTGGAGAGAA TCCTCCGTTT TCTGGAAGAC GAGATGCAAA ATGAGATTGT AGTTCAGTTG 
CTCGAGAAAA GCCCACTGAC CCCGCTCCCA CGGGAAATGA TTACCCTGGA GACTGTTCTA 
GAAAAACTGG AAGGAGAGTT ACAGGAAGCC AACCAGAACC AGCAGGCCTT GAAACAAAGC 
TTCCTAGAAC TGACAGAACT GAAATACCTC CTGAAGAAAA CCCAAGACTT CTTTGAGACG 
GAAACCAATT TAGCTGATGA TTTCTTTACT GAGGACACTT CTGGCCTCCT GGAGTTGAAA 
GCAGTGCCTG CATATATGAC CGGAAAGTTG GGGTTCATAG CCGGTGTGAT CAACAGGGAG 
AGGATGGCTT CCTTTGAGCG GTTACTGTGG CGAATCTGCC GAGGAAACGT GTACTTGAAG 
TTCAGTGAGA TGGACGCCCC TCTGGAGGAT CCTGTGACGA AAGAAGAAAT TCAGAAGAAC 
ATATTCATCA TATTTTACCA AGGAGAGCAG CTCAGGCAGA AAATCAAGAA GATCTGTGAT 
GGGTTTCGAG CCACTGTCTA CCCTTGCCCA GAGCCTGCGG TGGAGCGCAG AGAGATGTTG 
GAGAGCGTCA ATGTGAGGCT GGAAGATTTA ATCACCGTCA TAACACAAAC AGAGTCTCAC 
CGCCAGCGCC TGCTGCAGGA AGCCGCTGCC AACTGGCACT CCTGGCTCAT CAAGGTGCAG 
AAGATGAAAG CTGTCTACCA CATCCTGAAC ATGTGCAACA TCGACGTCAC CCAGCAGTGT 
GTCATCGCCG AGATCTGGTT CCCGGTGGCA GATGCCACAC GTATCAAGAG GGCACTGGAG 
CAAGGCATGG AACTAAGTGG CTCCTCCATG GCCCCCATCA TGACCACAGT GCAATCTAAA 
ACAGCCCCTC CCACATTTAA CAGGACCAAT AAATTCACAG CTGGCTTCCA GAATATTGTT 
GATGCCTATG GTGTCGGCAG CTACCGGGAG ATAAACCCAG CCCCCTACAC CATCATCACT 
TTCCCCTTCC TGTTCGCTGT GATGTTTGGA GACTGTGGTC ATGGAACCGT GATGCTCCTG 
GCTGCACTTT GGATGATTCT GAATGAGAGA CGCTTGCTCT CCCAGAAGAC AGACAATGAG 
ATTTGGAACA CCTTCTTCCA CGGGCGCTAT CTGATCCTAC TTATGGGCAT CTTCTCCATC 
TACACGGGTT TGATCTACAA TGACTGCTTC TCCAAGTCCT TGAACATCTT TGGCTCTTCT 
TGGAGTGTCC AACCCATGTT CAGAAACGGC ACATGGAATA CTCATGTAAT GGAGGAAAGT 
CTATATCTGC AGCTGGACCC AGCCATACCA GGAGTGTATT TTGGAAATCC ATACCCGTTT 
GGGATTGATC CGATTTGGAA CTTGGCTTCA AACAAACTCA CATTTCTGAA CTCGTATAAA 
ATGAAGATGT CGGTGATCCT GGGAATTGTC CAGATGGTTT TCGGTGTCAT CCTCAGCCTT 
TTCAATCACA TATACTTCAG AAGAACTCTC AACATCATTC TGCAATTTAT CCCTGAGATG 
ATTTTTATCC TGTGTCTGTT TGGATACCTG GTTTTCATGA TCATTTTCAA ATGGTGCTGC 
TTTGACGTCC ATGTATCTCA GCACGCCCCC AGCATCCTCA TCCACTTCAT CAACATGTTT 
CTGTTTAACT ACAGTGACTC TTCCAACGCA CCCCTCTACA AACATCAGCA AGAAGTCCAA 
AGTTTCTTTG TGGTTATGGC TTTGATTTCT GTGCCGTGGA TGCTTCTGAT TAAGCCGTTT 
ATTCTTAGAG CCAGTCATCG GAAATCCCAG CTGCAGGCAT CCAGGATCCA AGAAGATGCC 
ACTGAGAACA TTGAAGGTGA TAGCTCCAGC CCTTCTAGCC GTTCTGGCCA GAGGACTTCT 
GCAGATACCC ACGGGGCTCT GGACGACCAT GGAGAAGAGT TCAACTTTGG AGACGTCTTT 
GTCCACCAAG CCATCCACAC CATCGAGTAC TGCCTGGGCT GCATTTCAAA CACAGCCTCC 
TACCTGCGGC TCTGGGCCCT CAGCCTGGCT CATGCACAAC TGTCTGAAGT GCTCTGGACT 
ATGGTGATGA ACAGCGGCCT TCAGACGCGA GGCTGGGGAG GAATCGTCGG GGTTTTTATT 
ATTTTTGCCG TATTTGCTGT CCTGACAGTA GCCATCCTTC TGATCATGGA GGGCCTCTCT 
GCTTTCCTGC ACGCCCTGCG ACTGCACTGG GTTGAGTTCC AGAACAAGTT CTATGTCGGG 
GATGGTTACA AGTTTTCTCC ATTCTCCTTT AAACACATCC TGGATGGCAC AGCCGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Distal renal tubular acidosis in a Libyan patient: Evidence for digenic inheritance.
European journal of medical genetics61(1)1-7(2018 Jan)
Nagara M,Papagregoriou G,Ben Abdallah R,Landoulsi Z,Bouyacoub Y,Elouej S,Kefi R,Pippucci T,Voskarides K,Bashamboo A,McElreavey K,Hachicha M,Romeo G,Seri M,Deltas C,Abdelhak S

The genetic and clinical spectrum of a large cohort of patients with distal renal tubular acidosis.
Kidney international91(5)1243-1255(2017 May)
Palazzo V,Provenzano A,Becherucci F,Sansavini G,Mazzinghi B,Orlandini V,Giunti L,Roperto RM,Pantaleo M,Artuso R,Andreucci E,Bargiacchi S,Traficante G,Stagi S,Murer L,Benetti E,Emma F,Giordano M,Rivieri F,Colussi G,Penco S,Manfredini E,Caruso MR,Garavelli L,Andrulli S,Vergine G,Miglietti N,Mancini E,Malaventura C,Percesepe A,Grosso E,Materassi M,Romagnani P,Giglio S

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ATP6V0A4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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