ATP5F1B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP5F1B
Entrez Gene ID	506
Full Name	ATP synthase F1 subunit beta
Synonyms	ATP5B,ATPMB,ATPSB,HEL-S-271
General protein information	Preferred Names ATP synthase F1 subunit beta Names ATP synthase subunit beta, mitochondrial ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide epididymis secretory protein Li 271 mitochondrial ATP synthase beta subunit mitochondrial ATP synthetase, beta subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.3
Summary	This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008].
Disorder MIM:	102910

mRNA and Protein(s)

mRNA	Protein	Name
NM_001686.3	NP_001677.2	ATP synthase subunit beta, mitochondrial precursor
NM_001686.4	NP_001677.2	ATP synthase subunit beta, mitochondrial precursor

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	ATPsyn-beta	NP_001259081.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012106	XP_320423.4
Saccharomyces cerevisiae (baker's yeast)	ATP2	NP_012655.3
Arabidopsis thaliana (thale cress)	AT5G08670	NP_568203.2
	ATP5B	XP_001091520.1
Bos taurus (cattle)	ATP5B	NP_786990.1
Mus musculus (house mouse)	Atp5b	NP_058054.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012081	XP_320446.2
Eremothecium gossypii	AGOS_ABR169W	NP_983117.2
Schizosaccharomyces pombe (fission yeast)	atp2	NP_593151.1
Magnaporthe oryzae (rice blast fungus)	MGG_03185	XP_003716804.1
Arabidopsis thaliana (thale cress)	AT5G08680	NP_680155.1
Arabidopsis thaliana (thale cress)	AT5G08690	NP_568204.1
Oryza sativa (rice)	Os05g0553000	NP_001056261.1
Pan troglodytes (chimpanzee)	ATP5B	XP_509149.2
Canis lupus familiaris (dog)	ATP5B	XP_531639.2
Danio rerio (zebrafish)	atp5b	NP_001019600.2
Kluyveromyces lactis	KLLA0D10703g	XP_453538.1
Neurospora crassa	NCU05430	XP_963253.2
Homo sapiens (human)	ATP5B	NP_001677.2
Rattus norvegicus (Norway rat)	Atp5b	NP_599191.1
Gallus gallus (chicken)	ATP5B	NP_001026562.2
Caenorhabditis elegans (roundworm)	atp-2	NP_498111.2
Oryza sativa (rice)	Os01g0685800	NP_001043900.1
Xenopus tropicalis (tropical clawed frog)	atp5b	NP_001001256.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that the relationship between ATPsyn-beta and insulin secretion deficiency suggests that ATPsyn-beta potentially could serve as a marker for type 2 diabetes mellitus disease risk in women with polycystic ovary syndrome.
Title: ATP synthase ?-subunit abnormality in pancreas islets of rats with polycystic ovary syndrome and type 2 diabetes mellitus.

ATP5B, as a binding partner of a metastasis-related short peptide (B04) on prostate cancer cells, is involved in promoting prostate cancer metastasis.
Title: Ectopic expression of the ATP synthase ? subunit on the membrane of PC-3M cells supports its potential role in prostate cancer metastasis.

In this instance, the ATP5B/CALR/HSP90B1/HSPB1/HSPD1-signaling network was revealed as the predominant target which was associated with the majority of the observed protein-protein interactions. As a result, the identified targets may be useful in explaining the anticancer mechanisms of ursolic acid and as potential targets for colorectal cancer therapy.
Title: Comparative proteomics-network analysis of proteins responsible for ursolic acid-induced cytotoxicity in colorectal cancer cells.

These results suggested that increasing levels of ATP5B and ETFB were associated with worsening renal injury.
Title: ATP5B and ETFB metabolic markers in children with congenital hydronephrosis.

experiments implicate circulating NEFA in obesity in suppressing muscle protein metabolism, and establish impaired beta-F1-ATPase translation as an important consequence of obesity
Title: Prolonged Exposure of Primary Human Muscle Cells to Plasma Fatty Acids Associated with Obese Phenotype Induces Persistent Suppression of Muscle Mitochondrial ATP Synthase ? Subunit.

The following ATP5F1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP5F1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24954	NM_001686.4 Latest version!	Homo sapiens ATP synthase F1 subunit beta (ATP5F1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
View Old Accession Versions >>
OHu24954	NM_001686.3	Homo sapiens ATP synthase F1 subunit beta (ATP5F1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP5F1B ( NM_001686.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001677.2 Homo sapiens ATP synthase F1 subunit beta (ATP5F1B), mRNA.

Price & Availability↑

CloneID	OHu24954
Clone ID Related Accession (Same CDS sequence)	NM_001686.3 , NM_001686.4
Accession Version	NM_001686.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-23
Organism	Homo sapiens(human)
Product	ATP synthase subunit beta, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI561028.1 and BC016512.1. On Jul 16, 2004 this sequence version replaced NM_001686.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ224288.1, SRR1660809.46869.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTGGGGT TTGTGGGTCG GGTGGCCGCT GCTCCGGCCT CCGGGGCCTT GCGGAGACTC 
ACCCCTTCAG CGTCGCTGCC CCCAGCTCAG CTCTTACTGC GGGCCGCTCC GACGGCGGTC 
CATCCTGTCA GGGACTATGC GGCGCAAACA TCTCCTTCGC CAAAAGCAGG CGCCGCCACC 
GGGCGCATCG TGGCGGTCAT TGGCGCAGTG GTGGACGTCC AGTTTGATGA GGGACTACCA 
CCAATTCTAA ATGCCCTGGA AGTGCAAGGC AGGGAGACCA GACTGGTTTT GGAGGTGGCC 
CAGCATTTGG GTGAGAGCAC AGTAAGGACT ATTGCTATGG ATGGTACAGA AGGCTTGGTT 
AGAGGCCAGA AAGTACTGGA TTCTGGTGCA CCAATCAAAA TTCCTGTTGG TCCTGAGACT 
TTGGGCAGAA TCATGAATGT CATTGGAGAA CCTATTGATG AAAGAGGTCC CATCAAAACC 
AAACAATTTG CTCCCATTCA TGCTGAGGCT CCAGAGTTCA TGGAAATGAG TGTTGAGCAG 
GAAATTCTGG TGACTGGTAT CAAGGTTGTC GATCTGCTAG CTCCCTATGC CAAGGGTGGC 
AAAATTGGGC TTTTTGGTGG TGCTGGAGTT GGCAAGACTG TACTGATCAT GGAGTTAATC 
AACAATGTCG CCAAAGCCCA TGGTGGTTAC TCTGTGTTTG CTGGTGTTGG TGAGAGGACC 
CGTGAAGGCA ATGATTTATA CCATGAAATG ATTGAATCTG GTGTTATCAA CTTAAAAGAT 
GCCACCTCTA AGGTAGCGCT GGTATATGGT CAAATGAATG AACCACCTGG TGCTCGTGCC 
CGGGTAGCTC TGACTGGGCT GACTGTGGCT GAATACTTCA GAGACCAAGA AGGTCAAGAT 
GTACTGCTAT TTATTGATAA CATCTTTCGC TTCACCCAGG CTGGTTCAGA GGTGTCTGCA 
TTATTGGGCC GAATCCCTTC TGCTGTGGGC TATCAGCCTA CCCTGGCCAC TGACATGGGT 
ACTATGCAGG AAAGAATTAC CACTACCAAG AAGGGATCTA TCACCTCTGT ACAGGCTATC 
TATGTGCCTG CTGATGACTT GACTGACCCT GCCCCTGCTA CTACGTTTGC CCATTTGGAT 
GCTACCACTG TACTGTCGCG TGCCATTGCT GAGCTGGGCA TCTATCCAGC TGTGGATCCT 
CTAGACTCCA CCTCTCGTAT CATGGATCCC AACATTGTTG GCAGTGAGCA TTACGATGTT 
GCCCGTGGGG TGCAAAAGAT CCTGCAGGAC TACAAATCCC TCCAGGATAT CATTGCCATC 
CTGGGTATGG ATGAACTTTC TGAGGAAGAC AAGTTGACCG TGTCCCGTGC ACGGAAAATA 
CAGCGTTTCT TGTCTCAGCC ATTCCAGGTT GCTGAGGTCT TCACAGGTCA TATGGGGAAG 
CTGGTACCCC TGAAGGAGAC CATCAAAGGA TTCCAGCAGA TTTTGGCAGG TGAATATGAC 
CATCTCCCAG AACAGGCCTT CTATATGGTG GGACCCATTG AAGAAGCTGT GGCAAAAGCT 
GATAAGCTGG CTGAAGAGCA TTCATCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001677.2
CDS	106..1695
Translation	MLGFVGRVAAAPASGALRRLTPSASLPPAQLLLRAAPTAVHPVRDYAAQTSPSPKAGAATGRIVAVIGAVVDVQFDEGLPPILNALEVQGRETRLVLEVAQHLGESTVRTIAMDGTEGLVRGQKVLDSGAPIKIPVGPETLGRIMNVIGEPIDERGPIKTKQFAPIHAEAPEFMEMSVEQEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHSS

Target ORF information:

RefSeq Version	NM_001686.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP synthase F1 subunit beta (ATP5F1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001686.3

ORF Insert Sequence:

ATGTTGGGGT TTGTGGGTCG GGTGGCCGCT GCTCCGGCCT CCGGGGCCTT GCGGAGACTC 
ACCCCTTCAG CGTCGCTGCC CCCAGCTCAG CTCTTACTGC GGGCCGCTCC GACGGCGGTC 
CATCCTGTCA GGGACTATGC GGCGCAAACA TCTCCTTCGC CAAAAGCAGG CGCCGCCACC 
GGGCGCATCG TGGCGGTCAT TGGCGCAGTG GTGGACGTCC AGTTTGATGA GGGACTACCA 
CCAATTCTAA ATGCCCTGGA AGTGCAAGGC AGGGAGACCA GACTGGTTTT GGAGGTGGCC 
CAGCATTTGG GTGAGAGCAC AGTAAGGACT ATTGCTATGG ATGGTACAGA AGGCTTGGTT 
AGAGGCCAGA AAGTACTGGA TTCTGGTGCA CCAATCAAAA TTCCTGTTGG TCCTGAGACT 
TTGGGCAGAA TCATGAATGT CATTGGAGAA CCTATTGATG AAAGAGGTCC CATCAAAACC 
AAACAATTTG CTCCCATTCA TGCTGAGGCT CCAGAGTTCA TGGAAATGAG TGTTGAGCAG 
GAAATTCTGG TGACTGGTAT CAAGGTTGTC GATCTGCTAG CTCCCTATGC CAAGGGTGGC 
AAAATTGGGC TTTTTGGTGG TGCTGGAGTT GGCAAGACTG TACTGATCAT GGAGTTAATC 
AACAATGTCG CCAAAGCCCA TGGTGGTTAC TCTGTGTTTG CTGGTGTTGG TGAGAGGACC 
CGTGAAGGCA ATGATTTATA CCATGAAATG ATTGAATCTG GTGTTATCAA CTTAAAAGAT 
GCCACCTCTA AGGTAGCGCT GGTATATGGT CAAATGAATG AACCACCTGG TGCTCGTGCC 
CGGGTAGCTC TGACTGGGCT GACTGTGGCT GAATACTTCA GAGACCAAGA AGGTCAAGAT 
GTACTGCTAT TTATTGATAA CATCTTTCGC TTCACCCAGG CTGGTTCAGA GGTGTCTGCA 
TTATTGGGCC GAATCCCTTC TGCTGTGGGC TATCAGCCTA CCCTGGCCAC TGACATGGGT 
ACTATGCAGG AAAGAATTAC CACTACCAAG AAGGGATCTA TCACCTCTGT ACAGGCTATC 
TATGTGCCTG CTGATGACTT GACTGACCCT GCCCCTGCTA CTACGTTTGC CCATTTGGAT 
GCTACCACTG TACTGTCGCG TGCCATTGCT GAGCTGGGCA TCTATCCAGC TGTGGATCCT 
CTAGACTCCA CCTCTCGTAT CATGGATCCC AACATTGTTG GCAGTGAGCA TTACGATGTT 
GCCCGTGGGG TGCAAAAGAT CCTGCAGGAC TACAAATCCC TCCAGGATAT CATTGCCATC 
CTGGGTATGG ATGAACTTTC TGAGGAAGAC AAGTTGACCG TGTCCCGTGC ACGGAAAATA 
CAGCGTTTCT TGTCTCAGCC ATTCCAGGTT GCTGAGGTCT TCACAGGTCA TATGGGGAAG 
CTGGTACCCC TGAAGGAGAC CATCAAAGGA TTCCAGCAGA TTTTGGCAGG TGAATATGAC 
CATCTCCCAG AACAGGCCTT CTATATGGTG GGACCCATTG AAGAAGCTGT GGCAAAAGCT 
GATAAGCTGG CTGAAGAGCA TTCATCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP5F1B ( NM_001686.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001677.2 Homo sapiens ATP synthase F1 subunit beta (ATP5F1B), mRNA.

Price & Availability↑

CloneID	OHu24954
Clone ID Related Accession (Same CDS sequence)	NM_001686.3 , NM_001686.4
Accession Version	NM_001686.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	ATP synthase subunit beta, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI561028.1 and BC016512.1. On Nov 22, 2018 this sequence version replaced NM_001686.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ224288.1, SRR3476690.1007849.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000262030.8/ ENSP00000262030.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTGGGGT TTGTGGGTCG GGTGGCCGCT GCTCCGGCCT CCGGGGCCTT GCGGAGACTC 
ACCCCTTCAG CGTCGCTGCC CCCAGCTCAG CTCTTACTGC GGGCCGCTCC GACGGCGGTC 
CATCCTGTCA GGGACTATGC GGCGCAAACA TCTCCTTCGC CAAAAGCAGG CGCCGCCACC 
GGGCGCATCG TGGCGGTCAT TGGCGCAGTG GTGGACGTCC AGTTTGATGA GGGACTACCA 
CCAATTCTAA ATGCCCTGGA AGTGCAAGGC AGGGAGACCA GACTGGTTTT GGAGGTGGCC 
CAGCATTTGG GTGAGAGCAC AGTAAGGACT ATTGCTATGG ATGGTACAGA AGGCTTGGTT 
AGAGGCCAGA AAGTACTGGA TTCTGGTGCA CCAATCAAAA TTCCTGTTGG TCCTGAGACT 
TTGGGCAGAA TCATGAATGT CATTGGAGAA CCTATTGATG AAAGAGGTCC CATCAAAACC 
AAACAATTTG CTCCCATTCA TGCTGAGGCT CCAGAGTTCA TGGAAATGAG TGTTGAGCAG 
GAAATTCTGG TGACTGGTAT CAAGGTTGTC GATCTGCTAG CTCCCTATGC CAAGGGTGGC 
AAAATTGGGC TTTTTGGTGG TGCTGGAGTT GGCAAGACTG TACTGATCAT GGAGTTAATC 
AACAATGTCG CCAAAGCCCA TGGTGGTTAC TCTGTGTTTG CTGGTGTTGG TGAGAGGACC 
CGTGAAGGCA ATGATTTATA CCATGAAATG ATTGAATCTG GTGTTATCAA CTTAAAAGAT 
GCCACCTCTA AGGTAGCGCT GGTATATGGT CAAATGAATG AACCACCTGG TGCTCGTGCC 
CGGGTAGCTC TGACTGGGCT GACTGTGGCT GAATACTTCA GAGACCAAGA AGGTCAAGAT 
GTACTGCTAT TTATTGATAA CATCTTTCGC TTCACCCAGG CTGGTTCAGA GGTGTCTGCA 
TTATTGGGCC GAATCCCTTC TGCTGTGGGC TATCAGCCTA CCCTGGCCAC TGACATGGGT 
ACTATGCAGG AAAGAATTAC CACTACCAAG AAGGGATCTA TCACCTCTGT ACAGGCTATC 
TATGTGCCTG CTGATGACTT GACTGACCCT GCCCCTGCTA CTACGTTTGC CCATTTGGAT 
GCTACCACTG TACTGTCGCG TGCCATTGCT GAGCTGGGCA TCTATCCAGC TGTGGATCCT 
CTAGACTCCA CCTCTCGTAT CATGGATCCC AACATTGTTG GCAGTGAGCA TTACGATGTT 
GCCCGTGGGG TGCAAAAGAT CCTGCAGGAC TACAAATCCC TCCAGGATAT CATTGCCATC 
CTGGGTATGG ATGAACTTTC TGAGGAAGAC AAGTTGACCG TGTCCCGTGC ACGGAAAATA 
CAGCGTTTCT TGTCTCAGCC ATTCCAGGTT GCTGAGGTCT TCACAGGTCA TATGGGGAAG 
CTGGTACCCC TGAAGGAGAC CATCAAAGGA TTCCAGCAGA TTTTGGCAGG TGAATATGAC 
CATCTCCCAG AACAGGCCTT CTATATGGTG GGACCCATTG AAGAAGCTGT GGCAAAAGCT 
GATAAGCTGG CTGAAGAGCA TTCATCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001677.2
CDS	22..1611
Translation	MLGFVGRVAAAPASGALRRLTPSASLPPAQLLLRAAPTAVHPVRDYAAQTSPSPKAGAATGRIVAVIGAVVDVQFDEGLPPILNALEVQGRETRLVLEVAQHLGESTVRTIAMDGTEGLVRGQKVLDSGAPIKIPVGPETLGRIMNVIGEPIDERGPIKTKQFAPIHAEAPEFMEMSVEQEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHSS

Target ORF information:

RefSeq Version	NM_001686.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP synthase F1 subunit beta (ATP5F1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001686.4

ORF Insert Sequence:

ATGTTGGGGT TTGTGGGTCG GGTGGCCGCT GCTCCGGCCT CCGGGGCCTT GCGGAGACTC 
ACCCCTTCAG CGTCGCTGCC CCCAGCTCAG CTCTTACTGC GGGCCGCTCC GACGGCGGTC 
CATCCTGTCA GGGACTATGC GGCGCAAACA TCTCCTTCGC CAAAAGCAGG CGCCGCCACC 
GGGCGCATCG TGGCGGTCAT TGGCGCAGTG GTGGACGTCC AGTTTGATGA GGGACTACCA 
CCAATTCTAA ATGCCCTGGA AGTGCAAGGC AGGGAGACCA GACTGGTTTT GGAGGTGGCC 
CAGCATTTGG GTGAGAGCAC AGTAAGGACT ATTGCTATGG ATGGTACAGA AGGCTTGGTT 
AGAGGCCAGA AAGTACTGGA TTCTGGTGCA CCAATCAAAA TTCCTGTTGG TCCTGAGACT 
TTGGGCAGAA TCATGAATGT CATTGGAGAA CCTATTGATG AAAGAGGTCC CATCAAAACC 
AAACAATTTG CTCCCATTCA TGCTGAGGCT CCAGAGTTCA TGGAAATGAG TGTTGAGCAG 
GAAATTCTGG TGACTGGTAT CAAGGTTGTC GATCTGCTAG CTCCCTATGC CAAGGGTGGC 
AAAATTGGGC TTTTTGGTGG TGCTGGAGTT GGCAAGACTG TACTGATCAT GGAGTTAATC 
AACAATGTCG CCAAAGCCCA TGGTGGTTAC TCTGTGTTTG CTGGTGTTGG TGAGAGGACC 
CGTGAAGGCA ATGATTTATA CCATGAAATG ATTGAATCTG GTGTTATCAA CTTAAAAGAT 
GCCACCTCTA AGGTAGCGCT GGTATATGGT CAAATGAATG AACCACCTGG TGCTCGTGCC 
CGGGTAGCTC TGACTGGGCT GACTGTGGCT GAATACTTCA GAGACCAAGA AGGTCAAGAT 
GTACTGCTAT TTATTGATAA CATCTTTCGC TTCACCCAGG CTGGTTCAGA GGTGTCTGCA 
TTATTGGGCC GAATCCCTTC TGCTGTGGGC TATCAGCCTA CCCTGGCCAC TGACATGGGT 
ACTATGCAGG AAAGAATTAC CACTACCAAG AAGGGATCTA TCACCTCTGT ACAGGCTATC 
TATGTGCCTG CTGATGACTT GACTGACCCT GCCCCTGCTA CTACGTTTGC CCATTTGGAT 
GCTACCACTG TACTGTCGCG TGCCATTGCT GAGCTGGGCA TCTATCCAGC TGTGGATCCT 
CTAGACTCCA CCTCTCGTAT CATGGATCCC AACATTGTTG GCAGTGAGCA TTACGATGTT 
GCCCGTGGGG TGCAAAAGAT CCTGCAGGAC TACAAATCCC TCCAGGATAT CATTGCCATC 
CTGGGTATGG ATGAACTTTC TGAGGAAGAC AAGTTGACCG TGTCCCGTGC ACGGAAAATA 
CAGCGTTTCT TGTCTCAGCC ATTCCAGGTT GCTGAGGTCT TCACAGGTCA TATGGGGAAG 
CTGGTACCCC TGAAGGAGAC CATCAAAGGA TTCCAGCAGA TTTTGGCAGG TGAATATGAC 
CATCTCCCAG AACAGGCCTT CTATATGGTG GGACCCATTG AAGAAGCTGT GGCAAAAGCT 
GATAAGCTGG CTGAAGAGCA TTCATCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

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ATP5F1B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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