AFDN cDNA ORF clone, Homo sapiens(human)

The following AFDN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFDN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu24568 NM_001291964.1
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$679.50-$951.30
$1359.00
OHu26785 NM_001040000.3
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$579.50-$811.30
$1159.00
OHu107064 NM_001366319.1
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu96620 NM_001366321.2
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu96623 XM_017010886.1
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu24535 NM_001207008.1
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu40829 XM_006715491.2
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu40832 XM_006715494.3
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu59006 XM_011535842.2
Latest version!
Homo sapiens myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu40825 NM_001366320.1
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu40825 XM_005266996.3
Latest version!
Homo sapiens myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu40826 XM_005266997.2
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu96615 XM_017010878.1
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu96616 XM_017010879.1
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu96617 XM_017010880.1
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu96618 XM_017010881.1
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu96619 XM_017010882.1
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu96620 XM_017010883.1
Latest version!
Homo sapiens myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu96621 XM_017010884.1
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu96622 XM_017010885.1
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu96624 XM_017010887.1
Latest version!
Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
View Old Accession Versions >>
OHu26785 NM_001040000.2 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$579.50-$811.30
$1159.00
OHu96620 NM_001366321.1 Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu24568
    Clone ID Related Accession (Same CDS sequence) NM_001291964.1
    Accession Version NM_001291964.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product afadin isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB621809.1, AL009178.4, AL049698.3 and AL161973.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB621809.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTAATC GAAACGCTCG CGGAGAAATT TCGACCTGAT
    ATGCGAATGC TGTCCTCTCC CAAGTATTCA CTCTATGAAG TGCATGTCAG CGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCTCTA GTTGTACAAC TGAATTGGAA CAAAGATGAT
    CGGGAAGGCA GATTTGTTCT TAAGAATGAG AATGACGCCA TTCCTCCTAA GAAGGCTCAA
    AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT
    GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG
    GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT
    ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT
    GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT
    TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC
    AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG
    GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTACGAAGA TGGTTCTGAC TCTAGAGATA AGCCAAAGCT TTACCGCCTT
    CAGTTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTGTTT
    GGCCCAGGAA TTCAGCCCCA TCACTGTGAC CTTACCAACA TGGATGGAGT GGTCACTGTG
    ACGCCCAGAA GTATGGACGC AGAAACCTAC GTGGAAGGCC AGCGCATCTC AGAAACCACC
    ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC
    CCCAGTCAGG ATCATGCTCT TGCAAAAAGA TCTGTGGATG GAGGCCTGAT GGTTAAGGGC
    CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTGGGAGG AGATATTCAT
    AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCG
    TCTGCCTCTA GCACAGCCGA GCGGGGAATG GTGAAGCCGA TGATCAGAGT AGAACAGCAG
    CCAGATTATC GCAGGCAAGA AAGCAGAACA CAGGATGCTT CTGGGCCTGA GCTGATACTA
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTGTCTGC CATTATAAAT
    TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA
    TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCTACAGA GCGCACACAT
    AAAGTCATTG CAGTCGTCAA CAAGATGGTG AGCATGATGG AGGGTGTCAT CCAGAAACAG
    AAGAATATTG CAGGGGCACT TGCCTTCTGG ATGGCAAATG CATCTGAACT TCTCAACTTC
    ATTAAGCAAG ACCGAGACCT TAGTCGGATC ACACTGGATG CTCAAGATGT TTTAGCACAT
    TTGGTTCAAA TGGCATTTAA ATACTTGGTT CACTGTCTTC AATCAGAACT TAATAATTAC
    ATGCCAGCCT TTCTAGATGA CCCTGAAGAG AACAGTCTGC AACGACCAAA AATAGATGAT
    GTGCTGCACA CGCTCACAGG AGCCATGTCC TTGCTACGAC GCTGCAGAGT CAATGCCGCC
    CTGACCATCC AGCTCTTCTC TCAGCTCTTC CACTTCATCA ATATGTGGCT GTTCAATAGA
    TTGGTGACCG ACCCAGATTC GGGGCTGTGC TCCCATTACT GGGGTGCGAT TATCCGTCAG
    CAGTTGGGCC ATATTGAAGC CTGGGCTGAG AAGCAGGGGC TGGAACTGGC TGCGGACTGT
    CATCTGAGCA GGATCGTGCA GGCAACGACT TTGCTTACCA TGGATAAGTA TGCACCTGAT
    GACATTCCAA ATATAAACAG CACCTGCTTT AAGTTAAATT CATTACAACT TCAAGCCTTA
    TTACAGAACT ATCACTGTGC ACCTGATGAG CCTTTTATCC CAACGGATCT TATAGAAAAT
    GTAGTGACTG TGGCTGAAAA CACTGCCGAT GAGCTGGCCC GCAGTGATGG AAGGGAAGTG
    CAGTTGGAGG AGGATCCTGA TCTGCAGCTG CCGTTTCTTT TGCCAGAAGA TGGTTATTCT
    TGTGATGTTG TCAGAAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TCTGTGCCAG
    AGAGGATTTT GCAGGTTAAT TCCTCACACA CGTTCACCAG GTACTTGGAC AATATATTTT
    GAAGGTGCAG ATTATGAAAG TCACCTTCTG CGTGAGAACA CAGAGCTGGC TCAGCCTCTG
    AGGAAAGAAC CTGAAATAAT CACTGTGACC CTAAAAAAGC AGAATGGAAT GGGCCTTAGC
    ATTGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTAGGAA TCTATGTGAA GTCGGTTGTG
    AAAGGAGGTG CTGCAGATGT GGATGGACGT CTAGCTGCAG GTGATCAGCT CCTCAGTGTG
    GATGGACGAA GTCTGGTTGG ACTCTCTCAG GAAAGGGCGG CAGAACTCAT GACAAGAACA
    AGCTCTGTGG TGACACTGGA AGTAGCAAAG CAGGGTGCCA TCTACCACGG TCTGGCCACC
    CTTCTCAATC AGCCATCCCC CATGATGCAG AGAATTTCAG ATCGTCGTGG CTCAGGTAAA
    CCCCGACCAA AGAGTGAAGG CTTTGAGCTC TATAATAATT CAACTCAAAA TGGGTCTCCT
    GAGAGTCCTC AGCTGCCTTG GGCAGAATAT AGTGAACCAA AGAAATTGCC TGGTGATGAC
    AGACTGATGA AAAATAGAGC TGATCACCGT TCCAGCCCCA ACGTAGCAAA TCAGCCTCCT
    AGTCCTGGAG GGAAAAGTGC ATATGCCTCT GGAACAACAG CGAAGATAAC ATCTGTCTCT
    ACTGGAAACC TCTGCACTGA GGAGCAGACG CCTCCGCCTA GACCTGAAGC CTACCCCATC
    CCCACTCAGA CGTACACCAG AGAGTATTTT ACCTTCCCAG CTTCCAAATC CCAGGATCGG
    ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TATGAGGAAA AGCCACATAT GCACACAGAT
    AGTAATCATT CCAGTATTGC AATTCAGCGT GTTACACGTT CCCAAGAAGA ACTTCGAGAA
    GATAAAGCTT ACCAACTTGA GCGGCATCGA ATAGAGGCAG CTATGGACCG AAAGTCTGAT
    AGTGATATGT GGATAAATCA GAGCTCCTCA CTGGACTCCA GTACCTCTAG CCAGGAGCAT
    CTGAACCATT CCTCTAAGTC GGTCACCCCT GCTTCCACAC TGACCAAAAG TGGCCCTGGC
    CGTTGGAAAA CACCAGCAGC CATACCGGCC ACCCCTGTGG CCGTCTCCCA GCCAATCCGA
    ACAGACCTGC CTCCGCCACC CCCGCCACCT CCAGTCCACT ATGCCGGTGA TTTCGATGGA
    ATGTCCATGG ATTTGCCTCT CCCACCACCC CCTTCCGCCA ACCAGATAGG GCTGCCGTCT
    GCGCAGGTGG CTGCTGCTGA ACGGAGAAAG AGAGAAGAAC ATCAGCGTTG GTATGAGAAG
    GAGAAGGCCC GCCTGGAGGA GGAGCGGGAG AGGAAGCGGA GAGAGCAGGA GAGGAAGTTG
    GGCCAGATGC GCACTCAGTC CTTAAACCCT GCTCCGTTTT CTCCCCTGAC TGCACAGCAG
    ATGAAGCCCG AAAAGCCTTC CACACTCCAG CGGCCACAGG AAACAGTCAT TCGGGAGCTG
    CAGCCTCAGC AGCAGCCCCG CACGATCGAG CGCAGAGACT TGCAGTACAT TACAGTCAGC
    AAAGAGGAGC TTTCCTCGGG GGACAGTCTG TCCCCCGACC CGTGGAAGCG GGACGCCAAG
    GAGAAGCTGG AGAAGCAGCA GCAGATGCAC ATCGTGGACA TGCTGAGCAA GGAGATCCAG
    GAGCTCCAGA GCAAACCGGA CCGCAGCGCC GAGGAGAGCG ACCGGCTGCG CAAGCTCATG
    CTGGAGTGGC AGTTCCAGAA GAGACTCCAG GAGTCGAAGC AGAAGGACGA AGATGACGAG
    GAGGAGGAGG ACGATGATGT GGACACCATG CTGATCATGC AGCGCCTGGA GGCTGAACGA
    AGAGCGAGGG ACGAGGAGCG GAGGCGGCAG CAGCAGTTAG AAGAGATGCG CAAGCGGGAA
    GCGGAAGACC GAGCGAGGCA AGAGGAAGAG CGCCGGCGGC AGGAGGAGGA GCGAACAAAA
    CGAGACGCTG AAGAAAAGAG GCGACAGGAA GAAGGGTATT ACAGCCGCCT GGAAGCCGAG
    AGGCGCAGAC AGCACGACGA GGCGGCGCGC AGGTTGCTGG AGCCCGAGGC GCCCGGTCTG
    TGCCGCCCTC CGCTTCCCCG GGACTACGAG CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC
    CCTCCTCCCC CGCCTCAGCG AAACGCCTCC TACCTCAAAA CACAGGTCCT CTCCCCCGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCATAC AATGAGGAGG AGGAGGAGGA GGACTGCAGC
    CTAGCAGGAC CAAACTCTTA CCCAGGATCT ACTGGAGCAG CTGTTGGAGC CCATGACGCC
    TGTCGGGATG CAAAAGAGAA GCGCTCTAAA AGCCAAGATG CAGATTCACC TGGAAGTTCT
    GGGGCCCCTG AAAACTTGAC ATTCAAGGAA CGCCAGCGTC TTTTTTCACA AGGTCAAGAT
    GTATCCAATA AAGTGAAAGC TTCTCGTAAA TTAACAGAAC TGGAGAATGA ACTGAACACA
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278893.1
    CDS320..5665
    Translation

    Target ORF information:

    RefSeq Version NM_001291964.1
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291964.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTAATC GAAACGCTCG CGGAGAAATT TCGACCTGAT
    ATGCGAATGC TGTCCTCTCC CAAGTATTCA CTCTATGAAG TGCATGTCAG CGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCTCTA GTTGTACAAC TGAATTGGAA CAAAGATGAT
    CGGGAAGGCA GATTTGTTCT TAAGAATGAG AATGACGCCA TTCCTCCTAA GAAGGCTCAA
    AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT
    GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG
    GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT
    ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT
    GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT
    TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC
    AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG
    GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTACGAAGA TGGTTCTGAC TCTAGAGATA AGCCAAAGCT TTACCGCCTT
    CAGTTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTGTTT
    GGCCCAGGAA TTCAGCCCCA TCACTGTGAC CTTACCAACA TGGATGGAGT GGTCACTGTG
    ACGCCCAGAA GTATGGACGC AGAAACCTAC GTGGAAGGCC AGCGCATCTC AGAAACCACC
    ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC
    CCCAGTCAGG ATCATGCTCT TGCAAAAAGA TCTGTGGATG GAGGCCTGAT GGTTAAGGGC
    CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTGGGAGG AGATATTCAT
    AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCG
    TCTGCCTCTA GCACAGCCGA GCGGGGAATG GTGAAGCCGA TGATCAGAGT AGAACAGCAG
    CCAGATTATC GCAGGCAAGA AAGCAGAACA CAGGATGCTT CTGGGCCTGA GCTGATACTA
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTGTCTGC CATTATAAAT
    TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA
    TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCTACAGA GCGCACACAT
    AAAGTCATTG CAGTCGTCAA CAAGATGGTG AGCATGATGG AGGGTGTCAT CCAGAAACAG
    AAGAATATTG CAGGGGCACT TGCCTTCTGG ATGGCAAATG CATCTGAACT TCTCAACTTC
    ATTAAGCAAG ACCGAGACCT TAGTCGGATC ACACTGGATG CTCAAGATGT TTTAGCACAT
    TTGGTTCAAA TGGCATTTAA ATACTTGGTT CACTGTCTTC AATCAGAACT TAATAATTAC
    ATGCCAGCCT TTCTAGATGA CCCTGAAGAG AACAGTCTGC AACGACCAAA AATAGATGAT
    GTGCTGCACA CGCTCACAGG AGCCATGTCC TTGCTACGAC GCTGCAGAGT CAATGCCGCC
    CTGACCATCC AGCTCTTCTC TCAGCTCTTC CACTTCATCA ATATGTGGCT GTTCAATAGA
    TTGGTGACCG ACCCAGATTC GGGGCTGTGC TCCCATTACT GGGGTGCGAT TATCCGTCAG
    CAGTTGGGCC ATATTGAAGC CTGGGCTGAG AAGCAGGGGC TGGAACTGGC TGCGGACTGT
    CATCTGAGCA GGATCGTGCA GGCAACGACT TTGCTTACCA TGGATAAGTA TGCACCTGAT
    GACATTCCAA ATATAAACAG CACCTGCTTT AAGTTAAATT CATTACAACT TCAAGCCTTA
    TTACAGAACT ATCACTGTGC ACCTGATGAG CCTTTTATCC CAACGGATCT TATAGAAAAT
    GTAGTGACTG TGGCTGAAAA CACTGCCGAT GAGCTGGCCC GCAGTGATGG AAGGGAAGTG
    CAGTTGGAGG AGGATCCTGA TCTGCAGCTG CCGTTTCTTT TGCCAGAAGA TGGTTATTCT
    TGTGATGTTG TCAGAAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TCTGTGCCAG
    AGAGGATTTT GCAGGTTAAT TCCTCACACA CGTTCACCAG GTACTTGGAC AATATATTTT
    GAAGGTGCAG ATTATGAAAG TCACCTTCTG CGTGAGAACA CAGAGCTGGC TCAGCCTCTG
    AGGAAAGAAC CTGAAATAAT CACTGTGACC CTAAAAAAGC AGAATGGAAT GGGCCTTAGC
    ATTGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTAGGAA TCTATGTGAA GTCGGTTGTG
    AAAGGAGGTG CTGCAGATGT GGATGGACGT CTAGCTGCAG GTGATCAGCT CCTCAGTGTG
    GATGGACGAA GTCTGGTTGG ACTCTCTCAG GAAAGGGCGG CAGAACTCAT GACAAGAACA
    AGCTCTGTGG TGACACTGGA AGTAGCAAAG CAGGGTGCCA TCTACCACGG TCTGGCCACC
    CTTCTCAATC AGCCATCCCC CATGATGCAG AGAATTTCAG ATCGTCGTGG CTCAGGTAAA
    CCCCGACCAA AGAGTGAAGG CTTTGAGCTC TATAATAATT CAACTCAAAA TGGGTCTCCT
    GAGAGTCCTC AGCTGCCTTG GGCAGAATAT AGTGAACCAA AGAAATTGCC TGGTGATGAC
    AGACTGATGA AAAATAGAGC TGATCACCGT TCCAGCCCCA ACGTAGCAAA TCAGCCTCCT
    AGTCCTGGAG GGAAAAGTGC ATATGCCTCT GGAACAACAG CGAAGATAAC ATCTGTCTCT
    ACTGGAAACC TCTGCACTGA GGAGCAGACG CCTCCGCCTA GACCTGAAGC CTACCCCATC
    CCCACTCAGA CGTACACCAG AGAGTATTTT ACCTTCCCAG CTTCCAAATC CCAGGATCGG
    ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TATGAGGAAA AGCCACATAT GCACACAGAT
    AGTAATCATT CCAGTATTGC AATTCAGCGT GTTACACGTT CCCAAGAAGA ACTTCGAGAA
    GATAAAGCTT ACCAACTTGA GCGGCATCGA ATAGAGGCAG CTATGGACCG AAAGTCTGAT
    AGTGATATGT GGATAAATCA GAGCTCCTCA CTGGACTCCA GTACCTCTAG CCAGGAGCAT
    CTGAACCATT CCTCTAAGTC GGTCACCCCT GCTTCCACAC TGACCAAAAG TGGCCCTGGC
    CGTTGGAAAA CACCAGCAGC CATACCGGCC ACCCCTGTGG CCGTCTCCCA GCCAATCCGA
    ACAGACCTGC CTCCGCCACC CCCGCCACCT CCAGTCCACT ATGCCGGTGA TTTCGATGGA
    ATGTCCATGG ATTTGCCTCT CCCACCACCC CCTTCCGCCA ACCAGATAGG GCTGCCGTCT
    GCGCAGGTGG CTGCTGCTGA ACGGAGAAAG AGAGAAGAAC ATCAGCGTTG GTATGAGAAG
    GAGAAGGCCC GCCTGGAGGA GGAGCGGGAG AGGAAGCGGA GAGAGCAGGA GAGGAAGTTG
    GGCCAGATGC GCACTCAGTC CTTAAACCCT GCTCCGTTTT CTCCCCTGAC TGCACAGCAG
    ATGAAGCCCG AAAAGCCTTC CACACTCCAG CGGCCACAGG AAACAGTCAT TCGGGAGCTG
    CAGCCTCAGC AGCAGCCCCG CACGATCGAG CGCAGAGACT TGCAGTACAT TACAGTCAGC
    AAAGAGGAGC TTTCCTCGGG GGACAGTCTG TCCCCCGACC CGTGGAAGCG GGACGCCAAG
    GAGAAGCTGG AGAAGCAGCA GCAGATGCAC ATCGTGGACA TGCTGAGCAA GGAGATCCAG
    GAGCTCCAGA GCAAACCGGA CCGCAGCGCC GAGGAGAGCG ACCGGCTGCG CAAGCTCATG
    CTGGAGTGGC AGTTCCAGAA GAGACTCCAG GAGTCGAAGC AGAAGGACGA AGATGACGAG
    GAGGAGGAGG ACGATGATGT GGACACCATG CTGATCATGC AGCGCCTGGA GGCTGAACGA
    AGAGCGAGGG ACGAGGAGCG GAGGCGGCAG CAGCAGTTAG AAGAGATGCG CAAGCGGGAA
    GCGGAAGACC GAGCGAGGCA AGAGGAAGAG CGCCGGCGGC AGGAGGAGGA GCGAACAAAA
    CGAGACGCTG AAGAAAAGAG GCGACAGGAA GAAGGGTATT ACAGCCGCCT GGAAGCCGAG
    AGGCGCAGAC AGCACGACGA GGCGGCGCGC AGGTTGCTGG AGCCCGAGGC GCCCGGTCTG
    TGCCGCCCTC CGCTTCCCCG GGACTACGAG CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC
    CCTCCTCCCC CGCCTCAGCG AAACGCCTCC TACCTCAAAA CACAGGTCCT CTCCCCCGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCATAC AATGAGGAGG AGGAGGAGGA GGACTGCAGC
    CTAGCAGGAC CAAACTCTTA CCCAGGATCT ACTGGAGCAG CTGTTGGAGC CCATGACGCC
    TGTCGGGATG CAAAAGAGAA GCGCTCTAAA AGCCAAGATG CAGATTCACC TGGAAGTTCT
    GGGGCCCCTG AAAACTTGAC ATTCAAGGAA CGCCAGCGTC TTTTTTCACA AGGTCAAGAT
    GTATCCAATA AAGTGAAAGC TTCTCGTAAA TTAACAGAAC TGGAGAATGA ACTGAACACA
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26785
    Clone ID Related Accession (Same CDS sequence) NM_001040000.2 , NM_001040000.3
    Accession Version NM_001040000.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product afadin isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI271860.1, AB209420.1, AK055689.1, D60168.1 and BE503462.1. On Jun 12, 2010 this sequence version replaced NM_001040000.1. Transcript Variant: This variant (2) has several differences compared to variant 4. The resulting isoform (2) is longer at the N-terminus but has a shorter and distinct C-terminus compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA
    CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC
    TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA
    CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT
    TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT
    GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC
    GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT
    AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT
    CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC
    TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT
    GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC
    TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA
    AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA
    GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT
    TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT
    TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT
    CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT
    AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT
    GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA
    ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC
    ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT
    CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT
    GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT
    CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC
    TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC
    ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT
    CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA
    GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA
    GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT
    GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG
    CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT
    GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC
    CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT
    GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG
    TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG
    AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG
    TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG
    CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG
    CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC
    AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC
    AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC
    CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC
    ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC
    GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA
    CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG
    GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA
    AAACGAGACG CTGAAGAAAA GGTTATGGTC CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035089.1
    CDS143..5098
    Translation

    Target ORF information:

    RefSeq Version NM_001040000.2
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001040000.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA
    CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC
    TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA
    CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT
    TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT
    GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC
    GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT
    AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT
    CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC
    TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT
    GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC
    TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA
    AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA
    GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT
    TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT
    TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT
    CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT
    AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT
    GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA
    ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC
    ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT
    CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT
    GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT
    CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC
    TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC
    ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT
    CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA
    GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA
    GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT
    GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG
    CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT
    GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC
    CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT
    GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG
    TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG
    AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG
    TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG
    CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG
    CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC
    AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC
    AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC
    CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC
    ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC
    GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA
    CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG
    GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA
    AAACGAGACG CTGAAGAAAA GGTTATGGTC CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26785
    Clone ID Related Accession (Same CDS sequence) NM_001040000.2 , NM_001040000.3
    Accession Version NM_001040000.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product afadin isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL009178.4, AI271860.1, AB209420.1, AK055689.1, D60168.1 and BE503462.1. On Jun 2, 2019 this sequence version replaced NM_001040000.2. Transcript Variant: This variant (2) has several differences compared to variant 4. The resulting isoform (2) is longer at the N-terminus but has a shorter and distinct C-terminus compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA
    CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC
    TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA
    CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT
    TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT
    GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC
    GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT
    AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT
    CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC
    TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT
    GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC
    TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA
    AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA
    GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT
    TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT
    TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT
    CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT
    AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT
    GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA
    ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC
    ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT
    CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT
    GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT
    CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC
    TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC
    ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT
    CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA
    GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA
    GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT
    GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG
    CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT
    GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC
    CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT
    GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG
    TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG
    AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG
    TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG
    CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG
    CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC
    AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC
    AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC
    CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC
    ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC
    GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA
    CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG
    GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA
    AAACGAGACG CTGAAGAAAA GGTTATGGTC CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035089.1
    CDS223..5178
    Translation

    Target ORF information:

    RefSeq Version NM_001040000.3
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001040000.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA
    CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC
    TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA
    CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT
    TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT
    GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC
    GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT
    AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT
    CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC
    TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT
    GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC
    TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA
    AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA
    GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT
    TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT
    TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT
    CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT
    AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT
    GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA
    ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC
    ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT
    CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT
    GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT
    CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC
    TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC
    ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT
    CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA
    GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA
    GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT
    GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG
    CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT
    GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC
    CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT
    GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG
    TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG
    AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG
    TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG
    CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG
    CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC
    AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC
    AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC
    CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC
    ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC
    GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA
    CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG
    GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA
    AAACGAGACG CTGAAGAAAA GGTTATGGTC CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107064
    Clone ID Related Accession (Same CDS sequence) NM_001366319.1
    Accession Version NM_001366319.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product afadin isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL009178.4, KT258903.1 and AL049698.3. On Sep 29, 2018 this sequence version replaced XM_024446446.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KT258903.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA
    CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC
    TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA
    CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT
    TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT
    GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC
    GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT
    AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT
    CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC
    TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT
    GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC
    TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA
    AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA
    GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT
    TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT
    TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT
    CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT
    AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT
    GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA
    ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC
    ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT
    CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT
    GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT
    CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC
    TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC
    ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT
    CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA
    GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA
    GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT
    GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG
    CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT
    GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC
    CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT
    GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG
    TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG
    AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG
    TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG
    CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG
    CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC
    AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC
    AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC
    CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC
    ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC
    GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA
    CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG
    GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA
    AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC
    GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAGCCCGA GGCGCCCGGT
    CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC
    GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC
    GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC
    AGCCTAGCAG GACCAAACTC TTACCCAGGA TCTACTGGAG CAGCTGTTGG AGCCCATGAC
    GCCTGTCGGG ATGCAAAAGA GAAGCGCTCT AAAAGCCAAG ATGCAGATTC ACCTGGAAGT
    TCTGGGGCCC CTGAAAACTT GACATTCAAG GAACGCCAGC GTCTTTTTTC ACAAGGTCAA
    GATGTATCCA ATAAAGTGAA AGCTTCTCGT AAATTAACAG AACTGGAGAA TGAACTGAAC
    ACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001353248.1
    CDS570..6038
    Translation

    Target ORF information:

    RefSeq Version NM_001366319.1
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001366319.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA
    CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC
    TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA
    CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT
    TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT
    GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC
    GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT
    AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT
    CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC
    TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT
    GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC
    TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA
    AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA
    GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT
    TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT
    TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT
    CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT
    AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT
    GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA
    ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC
    ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT
    CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT
    GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT
    CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC
    TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC
    ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT
    CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA
    GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA
    GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT
    GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG
    CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT
    GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC
    CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT
    GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG
    TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG
    AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG
    TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG
    CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG
    CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC
    AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC
    AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC
    CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC
    ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC
    GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA
    CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG
    GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA
    AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC
    GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAGCCCGA GGCGCCCGGT
    CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC
    GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC
    GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC
    AGCCTAGCAG GACCAAACTC TTACCCAGGA TCTACTGGAG CAGCTGTTGG AGCCCATGAC
    GCCTGTCGGG ATGCAAAAGA GAAGCGCTCT AAAAGCCAAG ATGCAGATTC ACCTGGAAGT
    TCTGGGGCCC CTGAAAACTT GACATTCAAG GAACGCCAGC GTCTTTTTTC ACAAGGTCAA
    GATGTATCCA ATAAAGTGAA AGCTTCTCGT AAATTAACAG AACTGGAGAA TGAACTGAAC
    ACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96620
    Clone ID Related Accession (Same CDS sequence) NM_001366321.2 , XM_017010883.1 , NM_001366321.1
    Accession Version NM_001366321.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5454bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product afadin isoform 7
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AL009178.4, AL731868.5 and AL049698.3. On Nov 30, 2019 this sequence version replaced NM_001366321.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT
    AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT
    GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC
    ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC
    CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC
    CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT
    GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT
    GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG
    CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG
    ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT
    TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA
    TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT
    ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC
    AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG
    GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA
    CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC
    CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT
    AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT
    GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA
    GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC
    TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT
    TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA
    GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG
    CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC
    AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT
    GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA
    AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT
    GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC
    ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA
    ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA
    AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA
    ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT
    GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT
    GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT
    GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG
    GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC
    CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA
    GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT
    TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA
    GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT
    GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT
    GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC
    AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC
    CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT
    GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT
    GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT
    CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG
    TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA
    GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA
    CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT
    CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT
    GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG
    GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG
    TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT
    TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC
    CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG
    GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG
    CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG
    GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG
    AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT
    GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC
    GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA
    GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA
    GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG
    CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG
    CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG
    CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT
    GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA
    AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA
    AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA
    AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA
    GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001353250.1
    CDS223..5676
    Translation

    Target ORF information:

    RefSeq Version NM_001366321.2
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001366321.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT
    AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT
    GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC
    ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC
    CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC
    CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT
    GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT
    GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG
    CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG
    ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT
    TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA
    TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT
    ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC
    AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG
    GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA
    CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC
    CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT
    AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT
    GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA
    GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC
    TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT
    TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA
    GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG
    CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC
    AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT
    GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA
    AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT
    GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC
    ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA
    ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA
    AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA
    ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT
    GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT
    GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT
    GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG
    GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC
    CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA
    GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT
    TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA
    GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT
    GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT
    GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC
    AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC
    CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT
    GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT
    GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT
    CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG
    TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA
    GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA
    CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT
    CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT
    GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG
    GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG
    TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT
    TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC
    CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG
    GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG
    CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG
    GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG
    AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT
    GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC
    GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA
    GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA
    GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG
    CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG
    CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG
    CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT
    GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA
    AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA
    AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA
    AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA
    GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96623
    Clone ID Related Accession (Same CDS sequence) XM_017010886.1
    Accession Version XM_017010886.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5343bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product afadin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC
    CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG
    TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC
    ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA
    ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT
    GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT
    AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT
    ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA
    GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA
    CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG
    ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT
    ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT
    ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC
    ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG
    GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG
    GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA
    CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC
    TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC
    AGTCTGCAAC GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG
    CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC
    TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC
    CATTACTGGG GTGCGATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG
    CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG
    CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG
    TTAAATTCAT TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT
    TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG
    CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG
    TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT
    TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT
    GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA
    AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA
    CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA
    GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA
    AGGGCGGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG
    GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT
    AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT
    GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC
    AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA
    ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT
    CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC
    TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT
    GAGGAAAAGC CACATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT
    ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA
    GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG
    GACTCCAGTA CCTCTAGCCA GGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT
    TCCACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC
    CCTGTGGCCG TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA
    GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT
    TCCGCCAACC AGATAGGGCT GCCGTCTGCG CAGGTGGCTG CTGCTGAACG GAGAAAGAGA
    GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG
    AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT
    CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG
    CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC
    AGAGACTTGC AGTACATTAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC
    CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC
    GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG
    GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG
    TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGACG ATGATGTGGA CACCATGCTG
    ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT
    GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC
    AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG
    CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG
    GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA GCCCGAGGCG
    CCCGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT
    CGGGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG
    GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA
    TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866375.1
    CDS257..5599
    Translation

    Target ORF information:

    RefSeq Version XM_017010886.1
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010886.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC
    CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG
    TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC
    ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA
    ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT
    GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT
    AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT
    ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA
    GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA
    CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG
    ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT
    ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT
    ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC
    ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG
    GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG
    GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA
    CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC
    TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC
    AGTCTGCAAC GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG
    CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC
    TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC
    CATTACTGGG GTGCGATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG
    CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG
    CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG
    TTAAATTCAT TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT
    TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG
    CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG
    TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT
    TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT
    GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA
    AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA
    CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA
    GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA
    AGGGCGGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG
    GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT
    AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT
    GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC
    AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA
    ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT
    CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC
    TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT
    GAGGAAAAGC CACATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT
    ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA
    GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG
    GACTCCAGTA CCTCTAGCCA GGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT
    TCCACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC
    CCTGTGGCCG TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA
    GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT
    TCCGCCAACC AGATAGGGCT GCCGTCTGCG CAGGTGGCTG CTGCTGAACG GAGAAAGAGA
    GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG
    AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT
    CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG
    CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC
    AGAGACTTGC AGTACATTAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC
    CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC
    GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG
    GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG
    TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGACG ATGATGTGGA CACCATGCTG
    ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT
    GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC
    AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG
    CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG
    GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA GCCCGAGGCG
    CCCGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT
    CGGGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG
    GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA
    TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24535
    Clone ID Related Accession (Same CDS sequence) NM_001207008.1
    Accession Version NM_001207008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5232bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Homo sapiens(human)
    Product afadin isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE645141.1, AB209420.1, DA200453.1, DB079271.1, AL161973.1 and AL049698.3. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) has multiple differences compared to variant 4. The resulting isoform (1, also known as AF6i3) contains a C-terminal F-actin-binding site and has been shown to be involved in the regulation of epithelial wound closure and intercellular adhesion (PMID:16882694). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA
    AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT
    GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG
    GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT
    ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT
    GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT
    TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC
    AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG
    GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA
    AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC
    TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT
    GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC
    ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA
    TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC
    CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG
    GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC
    AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC
    AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGCTTCTGGG
    CCTGAGCTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG
    TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT
    GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT
    ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGT
    GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT
    GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA
    GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA
    GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAACGA
    CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC
    AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG
    TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT
    GCGATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA
    CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT
    AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCATTA
    CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG
    GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT
    GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA
    GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT
    TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG
    CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT
    GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT
    GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGATGGAC GTCTAGCTGC AGGTGATCAG
    CTCCTCAGTG TGGATGGACG AAGTCTGGTT GGACTCTCTC AGGAAAGGGC GGCAGAACTC
    ATGACAAGAA CAAGCTCTGT GGTGACACTG GAAGTAGCAA AGCAGGGTGC CATCTACCAC
    GGTCTGGCCA CCCTTCTCAA TCAGCCATCC CCCATGATGC AGAGAATTTC AGATCGTCGT
    GGCTCAGGTA AACCCCGACC AAAGAGTGAA GGCTTTGAGC TCTATAATAA TTCAACTCAA
    AATGGGTCTC CTGAGAGTCC TCAGCTGCCT TGGGCAGAAT ATAGTGAACC AAAGAAATTG
    CCTGGTGATG ACAGACTGAT GAAAAATAGA GCTGATCACC GTTCCAGCCC CAACGTAGCA
    AATCAGCCTC CTAGTCCTGG AGGGAAAAGT GCATATGCCT CTGGAACAAC AGCGAAGATA
    ACATCTGTCT CTACTGGAAA CCTCTGCACT GAGGAGCAGA CGCCTCCGCC TAGACCTGAA
    GCCTACCCCA TCCCCACTCA GACGTACACC AGAGAGTATT TTACCTTCCC AGCTTCCAAA
    TCCCAGGATC GGATGGCTCC TCCTCAGAAC CAGTGGCCAA ATTATGAGGA AAAGCCACAT
    ATGCACACAG ATAGTAATCA TTCCAGTATT GCAATTCAGC GTGTTACACG TTCCCAAGAA
    GAACTTCGAG AAGATAAAGC TTACCAACTT GAGCGGCATC GAATAGAGGC AGCTATGGAC
    CGAAAGTCTG ATAGTGATAT GTGGATAAAT CAGAGCTCCT CACTGGACTC CAGTACCTCT
    AGCCAGGAGC ATCTGAACCA TTCCTCTAAG TCGGTCACCC CTGCTTCCAC ACTGACCAAA
    AGTGGCCCTG GCCGTTGGAA AACACCAGCA GCCATACCGG CCACCCCTGT GGCCGTCTCC
    CAGCCAATCC GAACAGACCT GCCTCCGCCA CCCCCGCCAC CTCCAGTCCA CTATGCCGGT
    GATTTCGATG GAATGTCCAT GGATTTGCCT CTCCCACCAC CCCCTTCCGC CAACCAGATA
    GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT GAACGGAGAA AGAGAGAAGA ACATCAGCGT
    TGGTATGAGA AGGAGAAGGC CCGCCTGGAG GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG
    GAGAGGAAGT TGGGCCAGAT GCGCACTCAG TCCTTAAACC CTGCTCCGTT TTCTCCCCTG
    ACTGCACAGC AGATGAAGCC CGAAAAGCCT TCCACACTCC AGCGGCCACA GGAAACAGTC
    ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC CGCACGATCG AGCGCAGAGA CTTGCAGTAC
    ATTACAGTCA GCAAAGAGGA GCTTTCCTCG GGGGACAGTC TGTCCCCCGA CCCGTGGAAG
    CGGGACGCCA AGGAGAAGCT GGAGAAGCAG CAGCAGATGC ACATCGTGGA CATGCTGAGC
    AAGGAGATCC AGGAGCTCCA GAGCAAACCG GACCGCAGCG CCGAGGAGAG CGACCGGCTG
    CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG AAGAGACTCC AGGAGTCGAA GCAGAAGGAC
    GAAGATGACG AGGAGGAGGA GGACGATGAT GTGGACACCA TGCTGATCAT GCAGCGCCTG
    GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA
    GAGATGCGCA AGCGGGAAGC GGAAGACCGA GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG
    GAGGAGGAGC GAACAAAACG AGACGCTGAA GAAAAGAGGC GACAGGAAGA AGGGTATTAC
    AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGACGAGG CGGCGCGCAG GTTGCTGGAG
    CCCGAGGCGC CCGGACCAAA CTCTTACCCA GGATCTACTG GAGCAGCTGT TGGAGCCCAT
    GACGCCTGTC GGGATGCAAA AGAGAAGCGC TCTAAAAGCC AAGATGCAGA TTCACCTGGA
    AGTTCTGGGG CCCCTGAAAA CTTGACATTC AAGGAACGCC AGCGTCTTTT TTCACAAGGT
    CAAGATGTAT CCAATAAAGT GAAAGCTTCT CGTAAATTAA CAGAACTGGA GAATGAACTG
    AACACAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193937.1
    CDS143..5374
    Translation

    Target ORF information:

    RefSeq Version NM_001207008.1
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001207008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA
    AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT
    GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG
    GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT
    ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT
    GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT
    TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC
    AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG
    GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA
    AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC
    TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT
    GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC
    ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA
    TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC
    CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG
    GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC
    AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC
    AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGCTTCTGGG
    CCTGAGCTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG
    TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT
    GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT
    ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGT
    GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT
    GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA
    GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA
    GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAACGA
    CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC
    AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG
    TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT
    GCGATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA
    CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT
    AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCATTA
    CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG
    GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT
    GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA
    GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT
    TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG
    CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT
    GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT
    GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGATGGAC GTCTAGCTGC AGGTGATCAG
    CTCCTCAGTG TGGATGGACG AAGTCTGGTT GGACTCTCTC AGGAAAGGGC GGCAGAACTC
    ATGACAAGAA CAAGCTCTGT GGTGACACTG GAAGTAGCAA AGCAGGGTGC CATCTACCAC
    GGTCTGGCCA CCCTTCTCAA TCAGCCATCC CCCATGATGC AGAGAATTTC AGATCGTCGT
    GGCTCAGGTA AACCCCGACC AAAGAGTGAA GGCTTTGAGC TCTATAATAA TTCAACTCAA
    AATGGGTCTC CTGAGAGTCC TCAGCTGCCT TGGGCAGAAT ATAGTGAACC AAAGAAATTG
    CCTGGTGATG ACAGACTGAT GAAAAATAGA GCTGATCACC GTTCCAGCCC CAACGTAGCA
    AATCAGCCTC CTAGTCCTGG AGGGAAAAGT GCATATGCCT CTGGAACAAC AGCGAAGATA
    ACATCTGTCT CTACTGGAAA CCTCTGCACT GAGGAGCAGA CGCCTCCGCC TAGACCTGAA
    GCCTACCCCA TCCCCACTCA GACGTACACC AGAGAGTATT TTACCTTCCC AGCTTCCAAA
    TCCCAGGATC GGATGGCTCC TCCTCAGAAC CAGTGGCCAA ATTATGAGGA AAAGCCACAT
    ATGCACACAG ATAGTAATCA TTCCAGTATT GCAATTCAGC GTGTTACACG TTCCCAAGAA
    GAACTTCGAG AAGATAAAGC TTACCAACTT GAGCGGCATC GAATAGAGGC AGCTATGGAC
    CGAAAGTCTG ATAGTGATAT GTGGATAAAT CAGAGCTCCT CACTGGACTC CAGTACCTCT
    AGCCAGGAGC ATCTGAACCA TTCCTCTAAG TCGGTCACCC CTGCTTCCAC ACTGACCAAA
    AGTGGCCCTG GCCGTTGGAA AACACCAGCA GCCATACCGG CCACCCCTGT GGCCGTCTCC
    CAGCCAATCC GAACAGACCT GCCTCCGCCA CCCCCGCCAC CTCCAGTCCA CTATGCCGGT
    GATTTCGATG GAATGTCCAT GGATTTGCCT CTCCCACCAC CCCCTTCCGC CAACCAGATA
    GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT GAACGGAGAA AGAGAGAAGA ACATCAGCGT
    TGGTATGAGA AGGAGAAGGC CCGCCTGGAG GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG
    GAGAGGAAGT TGGGCCAGAT GCGCACTCAG TCCTTAAACC CTGCTCCGTT TTCTCCCCTG
    ACTGCACAGC AGATGAAGCC CGAAAAGCCT TCCACACTCC AGCGGCCACA GGAAACAGTC
    ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC CGCACGATCG AGCGCAGAGA CTTGCAGTAC
    ATTACAGTCA GCAAAGAGGA GCTTTCCTCG GGGGACAGTC TGTCCCCCGA CCCGTGGAAG
    CGGGACGCCA AGGAGAAGCT GGAGAAGCAG CAGCAGATGC ACATCGTGGA CATGCTGAGC
    AAGGAGATCC AGGAGCTCCA GAGCAAACCG GACCGCAGCG CCGAGGAGAG CGACCGGCTG
    CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG AAGAGACTCC AGGAGTCGAA GCAGAAGGAC
    GAAGATGACG AGGAGGAGGA GGACGATGAT GTGGACACCA TGCTGATCAT GCAGCGCCTG
    GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA
    GAGATGCGCA AGCGGGAAGC GGAAGACCGA GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG
    GAGGAGGAGC GAACAAAACG AGACGCTGAA GAAAAGAGGC GACAGGAAGA AGGGTATTAC
    AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGACGAGG CGGCGCGCAG GTTGCTGGAG
    CCCGAGGCGC CCGGACCAAA CTCTTACCCA GGATCTACTG GAGCAGCTGT TGGAGCCCAT
    GACGCCTGTC GGGATGCAAA AGAGAAGCGC TCTAAAAGCC AAGATGCAGA TTCACCTGGA
    AGTTCTGGGG CCCCTGAAAA CTTGACATTC AAGGAACGCC AGCGTCTTTT TTCACAAGGT
    CAAGATGTAT CCAATAAAGT GAAAGCTTCT CGTAAATTAA CAGAACTGGA GAATGAACTG
    AACACAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40829
    Clone ID Related Accession (Same CDS sequence) XM_006715491.2
    Accession Version XM_006715491.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product afadin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006715491.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC
    CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG
    TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC
    ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA
    ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT
    GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT
    AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT
    ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA
    GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA
    CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG
    ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT
    ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT
    ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC
    ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG
    GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG
    GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA
    CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC
    TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC
    AGTCTGCAAC GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG
    CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC
    TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC
    CATTACTGGG GTGCGATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG
    CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG
    CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG
    TTAAATTCAT TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT
    TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG
    CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG
    TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT
    TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT
    GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA
    AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA
    CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA
    GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA
    AGGGCGGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG
    GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT
    AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT
    GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC
    AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA
    ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT
    CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC
    TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT
    GAGGAAAAGC CACATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT
    ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA
    GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG
    GACTCCAGTA CCTCTAGCCA GGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT
    TCCACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC
    CCTGTGGCCG TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA
    GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT
    TCCGCCAACC AGATAGGGCT GCCGTCTGCG CAGGTGGCTG CTGCTGAACG GAGAAAGAGA
    GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG
    AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT
    CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG
    CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC
    AGAGACTTGC AGTACATTAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC
    CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC
    GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG
    GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG
    TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGACG ATGATGTGGA CACCATGCTG
    ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG
    CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA
    GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG
    GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG
    CGCAGGTTGC TGGAGCCCGA GGCGCCCGGA CCAAACTCTT ACCCAGGATC TACTGGAGCA
    GCTGTTGGAG CCCATGACGC CTGTCGGGAT GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT
    GCAGATTCAC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAGGA ACGCCAGCGT
    CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA
    CTGGAGAATG AACTGAACAC AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006715554.1
    CDS257..5563
    Translation

    Target ORF information:

    RefSeq Version XM_006715491.2
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006715491.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC
    CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG
    TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC
    ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA
    ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT
    GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT
    AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT
    ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA
    GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA
    CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG
    ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT
    ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT
    ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC
    ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG
    GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG
    GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA
    CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC
    TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC
    AGTCTGCAAC GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG
    CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC
    TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC
    CATTACTGGG GTGCGATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG
    CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG
    CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG
    TTAAATTCAT TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT
    TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG
    CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG
    TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT
    TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT
    GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA
    AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA
    CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA
    GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA
    AGGGCGGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG
    GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT
    AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT
    GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC
    AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA
    ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT
    CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC
    TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT
    GAGGAAAAGC CACATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT
    ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA
    GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG
    GACTCCAGTA CCTCTAGCCA GGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT
    TCCACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC
    CCTGTGGCCG TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA
    GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT
    TCCGCCAACC AGATAGGGCT GCCGTCTGCG CAGGTGGCTG CTGCTGAACG GAGAAAGAGA
    GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG
    AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT
    CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG
    CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC
    AGAGACTTGC AGTACATTAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC
    CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC
    GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG
    GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG
    TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGACG ATGATGTGGA CACCATGCTG
    ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG
    CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA
    GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG
    GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG
    CGCAGGTTGC TGGAGCCCGA GGCGCCCGGA CCAAACTCTT ACCCAGGATC TACTGGAGCA
    GCTGTTGGAG CCCATGACGC CTGTCGGGAT GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT
    GCAGATTCAC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAGGA ACGCCAGCGT
    CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA
    CTGGAGAATG AACTGAACAC AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40832
    Clone ID Related Accession (Same CDS sequence) XM_006715494.3
    Accession Version XM_006715494.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product afadin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006715494.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA
    AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT
    GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG
    GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT
    ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT
    GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT
    TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC
    AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG
    GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA
    AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC
    TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT
    GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC
    ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA
    TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC
    CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG
    GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC
    AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC
    AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGCTTCTGGG
    CCTGAGCTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG
    TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT
    GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT
    ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGT
    GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT
    GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA
    GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA
    GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAACGA
    CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC
    AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG
    TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT
    GCGATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA
    CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT
    AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCATTA
    CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG
    GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT
    GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA
    GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT
    TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG
    CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT
    GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT
    GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT
    CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCGGCAGAA
    CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC
    CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT
    CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT
    CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA
    TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA
    GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG
    ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT
    GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC
    AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCA
    CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA
    GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG
    GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC
    TCTAGCCAGG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC CACACTGACC
    AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCGTC
    TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC
    GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CGCCAACCAG
    ATAGGGCTGC CGTCTGCGCA GGTGGCTGCT GCTGAACGGA GAAAGAGAGA AGAACATCAG
    CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG
    CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC
    CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA
    GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG
    TACATTACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG
    AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG
    AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG
    CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG
    GACGAAGATG ACGAGGAGGA GGAGGACGAT GATGTGGACA CCATGCTGAT CATGCAGCGC
    CTGGAGGCTG AACGAAGAGC GAGGTTGCAG GACGAGGAGC GGAGGCGGCA GCAGCAGTTA
    GAAGAGATGC GCAAGCGGGA AGCGGAAGAC CGAGCGAGGC AAGAGGAAGA GCGCCGGCGG
    CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT
    TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGACG AGGCGGCGCG CAGGTTGCTG
    GAGCCCGAGG CGCCCGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC
    CATGACGCCT GTCGGGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT
    GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA
    GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA
    CTGAACACAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006715557.1
    CDS245..5479
    Translation

    Target ORF information:

    RefSeq Version XM_006715494.3
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006715494.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA
    AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT
    GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG
    GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT
    ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT
    GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT
    TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC
    AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG
    GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA
    AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC
    TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT
    GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC
    ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA
    TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC
    CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG
    GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC
    AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC
    AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGCTTCTGGG
    CCTGAGCTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG
    TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT
    GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT
    ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGT
    GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT
    GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA
    GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA
    GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAACGA
    CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC
    AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG
    TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT
    GCGATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA
    CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT
    AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCATTA
    CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG
    GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT
    GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA
    GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT
    TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG
    CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT
    GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT
    GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT
    CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCGGCAGAA
    CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC
    CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT
    CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT
    CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA
    TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA
    GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG
    ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT
    GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC
    AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCA
    CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA
    GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG
    GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC
    TCTAGCCAGG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC CACACTGACC
    AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCGTC
    TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC
    GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CGCCAACCAG
    ATAGGGCTGC CGTCTGCGCA GGTGGCTGCT GCTGAACGGA GAAAGAGAGA AGAACATCAG
    CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG
    CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC
    CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA
    GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG
    TACATTACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG
    AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG
    AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG
    CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG
    GACGAAGATG ACGAGGAGGA GGAGGACGAT GATGTGGACA CCATGCTGAT CATGCAGCGC
    CTGGAGGCTG AACGAAGAGC GAGGTTGCAG GACGAGGAGC GGAGGCGGCA GCAGCAGTTA
    GAAGAGATGC GCAAGCGGGA AGCGGAAGAC CGAGCGAGGC AAGAGGAAGA GCGCCGGCGG
    CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT
    TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGACG AGGCGGCGCG CAGGTTGCTG
    GAGCCCGAGG CGCCCGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC
    CATGACGCCT GTCGGGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT
    GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA
    GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA
    CTGAACACAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59006
    Clone ID Related Accession (Same CDS sequence) XM_011535842.2
    Accession Version XM_011535842.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product afadin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535842.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA
    CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC
    TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA
    CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT
    TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT
    GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC
    GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT
    AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT
    CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC
    TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT
    GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC
    TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA
    AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA
    GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT
    TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT
    TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT
    CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT
    AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT
    GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA
    ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC
    ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT
    CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT
    GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT
    CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC
    TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC
    ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT
    CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA
    GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA
    GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT
    GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG
    CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT
    GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC
    CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT
    GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG
    TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG
    AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG
    TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG
    CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG
    CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC
    AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC
    AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC
    CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC
    ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC
    GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA
    CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG
    GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA
    AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC
    GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAGCCCGA GGCGCCCGGA
    CCAAACTCTT ACCCAGGATC TACTGGAGCA GCTGTTGGAG CCCATGACGC CTGTCGGGAT
    GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT GCAGATTCAC CTGGAAGTTC TGGGGCCCCT
    GAAAACTTGA CATTCAAGGA ACGCCAGCGT CTTTTTTCAC AAGGTCAAGA TGTATCCAAT
    AAAGTGAAAG CTTCTCGTAA ATTAACAGAA CTGGAGAATG AACTGAACAC AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534144.1
    CDS262..5538
    Translation

    Target ORF information:

    RefSeq Version XM_011535842.2
    Organism Homo sapiens(human)
    Definition Homo sapiens myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011535842.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA
    CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC
    TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA
    CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT
    TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT
    GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC
    GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT
    AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT
    CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC
    TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT
    GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC
    TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA
    AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA
    GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT
    TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT
    TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT
    CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT
    AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT
    GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA
    ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC
    ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT
    CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT
    GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT
    CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC
    TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC
    ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT
    CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA
    GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA
    GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT
    GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG
    CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT
    GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC
    CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT
    GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG
    TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG
    AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG
    TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG
    CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG
    CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC
    AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC
    AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC
    CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC
    ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC
    GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA
    CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG
    GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA
    AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC
    GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAGCCCGA GGCGCCCGGA
    CCAAACTCTT ACCCAGGATC TACTGGAGCA GCTGTTGGAG CCCATGACGC CTGTCGGGAT
    GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT GCAGATTCAC CTGGAAGTTC TGGGGCCCCT
    GAAAACTTGA CATTCAAGGA ACGCCAGCGT CTTTTTTCAC AAGGTCAAGA TGTATCCAAT
    AAAGTGAAAG CTTCTCGTAA ATTAACAGAA CTGGAGAATG AACTGAACAC AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40825
    Clone ID Related Accession (Same CDS sequence) XM_005266996.3 , NM_001366320.1
    Accession Version NM_001366320.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5475bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product afadin isoform 6
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AL009178.4, AL731868.5 and AL049698.3. On Sep 29, 2018 this sequence version replaced XM_005266996.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA
    CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC
    TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA
    CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT
    TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT
    GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC
    GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT
    AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT
    CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC
    TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT
    GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC
    TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA
    AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA
    GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT
    TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT
    TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT
    CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT
    AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT
    GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA
    ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC
    ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT
    CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT
    GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT
    CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC
    TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC
    ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT
    CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA
    GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA
    GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT
    GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG
    CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT
    GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC
    CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT
    GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG
    TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG
    AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG
    TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG
    CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG
    CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC
    AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC
    AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC
    CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC
    ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC
    GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA
    CGAAGAGCGA GGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC
    AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG
    CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG
    GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA GCCCGAGGCG
    CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG
    CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC
    TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG
    GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC
    CATGACGCCT GTCGGGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT
    GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA
    GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA
    CTGAACACAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001353249.1
    CDS570..6044
    Translation

    Target ORF information:

    RefSeq Version NM_001366320.1
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001366320.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA
    CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC
    TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA
    CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT
    TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT
    GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC
    GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT
    AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT
    CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC
    TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT
    GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC
    TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA
    AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA
    GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT
    TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT
    TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT
    CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT
    AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT
    GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA
    ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC
    ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT
    CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT
    GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT
    CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC
    TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC
    ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT
    CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA
    GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA
    GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT
    GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG
    CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT
    GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC
    CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT
    GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG
    TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG
    AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG
    TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG
    CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG
    CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC
    AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC
    AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC
    CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC
    ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC
    GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA
    CGAAGAGCGA GGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC
    AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG
    CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG
    GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA GCCCGAGGCG
    CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG
    CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC
    TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG
    GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC
    CATGACGCCT GTCGGGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT
    GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA
    GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA
    CTGAACACAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96620
    Clone ID Related Accession (Same CDS sequence) NM_001366321.2 , XM_017010883.1 , NM_001366321.1
    Accession Version NM_001366321.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5454bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-05
    Organism Homo sapiens(human)
    Product afadin isoform 7
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AL009178.4, AL731868.5 and AL049698.3. On Sep 29, 2018 this sequence version replaced XM_017010883.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT
    AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT
    GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC
    ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC
    CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC
    CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT
    GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT
    GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG
    CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG
    ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT
    TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA
    TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT
    ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC
    AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG
    GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA
    CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC
    CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT
    AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT
    GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA
    GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC
    TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT
    TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA
    GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG
    CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC
    AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT
    GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA
    AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT
    GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC
    ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA
    ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA
    AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA
    ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT
    GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT
    GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT
    GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG
    GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC
    CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA
    GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT
    TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA
    GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT
    GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT
    GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC
    AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC
    CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT
    GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT
    GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT
    CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG
    TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA
    GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA
    CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT
    CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT
    GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG
    GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG
    TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT
    TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC
    CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG
    GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG
    CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG
    GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG
    AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT
    GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC
    GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA
    GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA
    GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG
    CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG
    CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG
    CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT
    GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA
    AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA
    AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA
    AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA
    GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001353250.1
    CDS570..6023
    Translation

    Target ORF information:

    RefSeq Version NM_001366321.1
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001366321.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT
    AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT
    GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC
    ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC
    CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC
    CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT
    GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT
    GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG
    CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG
    ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT
    TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA
    TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT
    ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC
    AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG
    GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA
    CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC
    CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT
    AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT
    GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA
    GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC
    TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT
    TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA
    GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG
    CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC
    AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT
    GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA
    AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT
    GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC
    ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA
    ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA
    AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA
    ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT
    GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT
    GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT
    GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG
    GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC
    CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA
    GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT
    TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA
    GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT
    GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT
    GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC
    AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC
    CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT
    GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT
    GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT
    CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG
    TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA
    GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA
    CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT
    CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT
    GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG
    GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG
    TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT
    TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC
    CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG
    GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG
    CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG
    GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG
    AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT
    GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC
    GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA
    GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA
    GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG
    CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG
    CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG
    CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT
    GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA
    AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA
    AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA
    AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA
    GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40825
    Clone ID Related Accession (Same CDS sequence) XM_005266996.3 , NM_001366320.1
    Accession Version XM_005266996.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5475bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product afadin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005266996.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA
    CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC
    TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA
    CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT
    TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT
    GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC
    GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT
    AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT
    CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC
    TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT
    GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC
    TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA
    AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA
    GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT
    TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT
    TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT
    CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT
    AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT
    GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA
    ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC
    ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT
    CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT
    GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT
    CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC
    TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC
    ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT
    CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA
    GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA
    GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT
    GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG
    CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT
    GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC
    CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT
    GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG
    TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG
    AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG
    TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG
    CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG
    CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC
    AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC
    AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC
    CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC
    ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC
    GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA
    CGAAGAGCGA GGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC
    AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG
    CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG
    GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA GCCCGAGGCG
    CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG
    CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC
    TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG
    GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC
    CATGACGCCT GTCGGGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT
    GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA
    GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA
    CTGAACACAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005267053.1
    CDS262..5736
    Translation

    Target ORF information:

    RefSeq Version XM_005266996.3
    Organism Homo sapiens(human)
    Definition Homo sapiens myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005266996.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGAAA
    CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC
    TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA
    CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT
    TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAACGACC AAAAATAGAT
    GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC
    GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT
    AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC GATTATCCGT
    CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC
    TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT
    GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCATTACA ACTTCAAGCC
    TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA
    AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA
    GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT
    TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT
    TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT
    CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT
    AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT
    GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CGGCAGAACT CATGACAAGA
    ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC
    ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT
    CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT
    GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT
    CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC
    TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC
    ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT
    CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCACA TATGCACACA
    GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA
    GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT
    GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAGGAG
    CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCCA CACTGACCAA AAGTGGCCCT
    GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCAATC
    CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT
    GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCG CCAACCAGAT AGGGCTGCCG
    TCTGCGCAGG TGGCTGCTGC TGAACGGAGA AAGAGAGAAG AACATCAGCG TTGGTATGAG
    AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG
    TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG
    CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG
    CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATTACAGTC
    AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC
    AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC
    CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC
    ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC
    GAGGAGGAGG AGGACGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA
    CGAAGAGCGA GGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC
    AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG
    CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG
    GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA GCCCGAGGCG
    CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG
    CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC
    TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG
    GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC
    CATGACGCCT GTCGGGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT
    GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA
    GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA
    CTGAACACAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40826
    Clone ID Related Accession (Same CDS sequence) XM_005266997.2
    Accession Version XM_005266997.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product afadin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005266997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC
    CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG
    TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC
    ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA
    ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT
    GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT
    AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT
    ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA
    GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA
    CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG
    ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT
    ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT
    ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC
    ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG
    AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC
    AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA
    GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT
    AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAACG ACCAAAAATA
    GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT
    GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC
    AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCGATTATC
    CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG
    GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA
    CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCATT ACAACTTCAA
    GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA
    GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG
    GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT
    TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG
    TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA
    TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG
    CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC
    CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG
    GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC
    AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCGGCAGA ACTCATGACA
    AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG
    GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA
    GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG
    TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT
    GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG
    CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT
    GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC
    CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG
    GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC ACATATGCAC
    ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT
    CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG
    TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAG
    GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CCACACTGAC CAAAAGTGGC
    CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCGT CTCCCAGCCA
    ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC
    GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCGCCAACCA GATAGGGCTG
    CCGTCTGCGC AGGTGGCTGC TGCTGAACGG AGAAAGAGAG AAGAACATCA GCGTTGGTAT
    GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG
    AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA
    CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG
    GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATTACA
    GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC
    GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG
    ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG
    CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT
    GACGAGGAGG AGGAGGACGA TGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT
    GAACGAAGAG CGAGGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG
    CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA
    ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA
    GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAGCC CGAGGCGCCC
    GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC
    GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC
    CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC
    TGCAGCCTAG CAGGACCAAA CTCTTACCCA GGATCTACTG GAGCAGCTGT TGGAGCCCAT
    GACGCCTGTC GGGATGCAAA AGAGAAGCGC TCTAAAAGCC AAGATGCAGA TTCACCTGGA
    AGTTCTGGGG CCCCTGAAAA CTTGACATTC AAGGAACGCC AGCGTCTTTT TTCACAAGGT
    CAAGATGTAT CCAATAAAGT GAAAGCTTCT CGTAAATTAA CAGAACTGGA GAATGAACTG
    AACACAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005267054.1
    CDS257..5728
    Translation

    Target ORF information:

    RefSeq Version XM_005266997.2
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005266997.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC
    CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG
    TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC
    ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA
    ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT
    GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT
    AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT
    ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA
    GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA
    CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG
    ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT
    ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT
    ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC
    ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG
    AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC
    AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA
    GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT
    AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAACG ACCAAAAATA
    GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT
    GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC
    AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCGATTATC
    CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG
    GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA
    CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCATT ACAACTTCAA
    GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA
    GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG
    GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT
    TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG
    TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA
    TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG
    CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC
    CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG
    GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC
    AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCGGCAGA ACTCATGACA
    AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG
    GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA
    GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG
    TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT
    GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG
    CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT
    GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC
    CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG
    GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC ACATATGCAC
    ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT
    CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG
    TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAG
    GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CCACACTGAC CAAAAGTGGC
    CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCGT CTCCCAGCCA
    ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC
    GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCGCCAACCA GATAGGGCTG
    CCGTCTGCGC AGGTGGCTGC TGCTGAACGG AGAAAGAGAG AAGAACATCA GCGTTGGTAT
    GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG
    AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA
    CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG
    GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATTACA
    GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC
    GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG
    ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG
    CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT
    GACGAGGAGG AGGAGGACGA TGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT
    GAACGAAGAG CGAGGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG
    CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA
    ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA
    GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAGCC CGAGGCGCCC
    GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC
    GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC
    CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC
    TGCAGCCTAG CAGGACCAAA CTCTTACCCA GGATCTACTG GAGCAGCTGT TGGAGCCCAT
    GACGCCTGTC GGGATGCAAA AGAGAAGCGC TCTAAAAGCC AAGATGCAGA TTCACCTGGA
    AGTTCTGGGG CCCCTGAAAA CTTGACATTC AAGGAACGCC AGCGTCTTTT TTCACAAGGT
    CAAGATGTAT CCAATAAAGT GAAAGCTTCT CGTAAATTAA CAGAACTGGA GAATGAACTG
    AACACAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96615
    Clone ID Related Accession (Same CDS sequence) XM_017010878.1
    Accession Version XM_017010878.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product afadin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC
    CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG
    TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC
    ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA
    ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT
    GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT
    AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT
    ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA
    GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA
    CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG
    ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT
    ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT
    ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC
    ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG
    GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG
    GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA
    CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC
    TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC
    AGTCTGCAAC GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG
    CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC
    TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC
    CATTACTGGG GTGCGATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG
    CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG
    CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG
    TTAAATTCAT TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT
    TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG
    CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG
    TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT
    TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT
    GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA
    AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA
    CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA
    GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA
    AGGGCGGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG
    GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT
    AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT
    GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC
    AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA
    ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT
    CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC
    TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT
    GAGGAAAAGC CACATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT
    ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA
    GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG
    GACTCCAGTA CCTCTAGCCA GGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT
    TCCACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC
    CCTGTGGCCG TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA
    GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT
    TCCGCCAACC AGATAGGGCT GCCGTCTGCG CAGGTGGCTG CTGCTGAACG GAGAAAGAGA
    GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG
    AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT
    CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG
    CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC
    AGAGACTTGC AGTACATTAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC
    CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC
    GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG
    GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG
    TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGACG ATGATGTGGA CACCATGCTG
    ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG
    CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA
    GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG
    GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG
    CGCAGGTTGC TGGAGCCCGA GGCGCCCGGT CTGTGCCGCC CTCCGCTTCC CCGGGACTAC
    GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC GCCCCTCCTC CCCCGCCTCA GCGAAACGCC
    TCCTACCTCA AAACACAGGT CCTCTCCCCC GACTCGCTGT TCACTGCCAA GTTTGTTGCA
    TACAATGAGG AGGAGGAGGA GGAGGACTGC AGCCTAGCAG GACCAAACTC TTACCCAGGA
    TCTACTGGAG CAGCTGTTGG AGCCCATGAC GCCTGTCGGG ATGCAAAAGA GAAGCGCTCT
    AAAAGCCAAG ATGCAGATTC ACCTGGAAGT TCTGGGGCCC CTGAAAACTT GACATTCAAG
    GAACGCCAGC GTCTTTTTTC ACAAGGTCAA GATGTATCCA ATAAAGTGAA AGCTTCTCGT
    AAATTAACAG AACTGGAGAA TGAACTGAAC ACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866367.1
    CDS257..5755
    Translation

    Target ORF information:

    RefSeq Version XM_017010878.1
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010878.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC
    CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG
    TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC
    ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA
    ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT
    GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT
    AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT
    ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA
    GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA
    CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG
    ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT
    ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT
    ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC
    ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG
    GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG
    GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA
    CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC
    TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC
    AGTCTGCAAC GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG
    CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC
    TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC
    CATTACTGGG GTGCGATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG
    CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG
    CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG
    TTAAATTCAT TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT
    TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG
    CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG
    TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT
    TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT
    GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA
    AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA
    CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA
    GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA
    AGGGCGGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG
    GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT
    AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT
    GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC
    AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA
    ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT
    CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC
    TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT
    GAGGAAAAGC CACATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT
    ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA
    GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG
    GACTCCAGTA CCTCTAGCCA GGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT
    TCCACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC
    CCTGTGGCCG TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA
    GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT
    TCCGCCAACC AGATAGGGCT GCCGTCTGCG CAGGTGGCTG CTGCTGAACG GAGAAAGAGA
    GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG
    AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT
    CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG
    CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC
    AGAGACTTGC AGTACATTAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC
    CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC
    GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG
    GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG
    TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGACG ATGATGTGGA CACCATGCTG
    ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG
    CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA
    GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG
    GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG
    CGCAGGTTGC TGGAGCCCGA GGCGCCCGGT CTGTGCCGCC CTCCGCTTCC CCGGGACTAC
    GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC GCCCCTCCTC CCCCGCCTCA GCGAAACGCC
    TCCTACCTCA AAACACAGGT CCTCTCCCCC GACTCGCTGT TCACTGCCAA GTTTGTTGCA
    TACAATGAGG AGGAGGAGGA GGAGGACTGC AGCCTAGCAG GACCAAACTC TTACCCAGGA
    TCTACTGGAG CAGCTGTTGG AGCCCATGAC GCCTGTCGGG ATGCAAAAGA GAAGCGCTCT
    AAAAGCCAAG ATGCAGATTC ACCTGGAAGT TCTGGGGCCC CTGAAAACTT GACATTCAAG
    GAACGCCAGC GTCTTTTTTC ACAAGGTCAA GATGTATCCA ATAAAGTGAA AGCTTCTCGT
    AAATTAACAG AACTGGAGAA TGAACTGAAC ACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96616
    Clone ID Related Accession (Same CDS sequence) XM_017010879.1
    Accession Version XM_017010879.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product afadin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGGAA
    GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA
    AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG
    GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT
    CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT
    CTGCAACGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA
    CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC
    ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT
    TACTGGGGTG CGATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG
    GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT
    ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA
    AATTCATTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT
    ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG
    GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT
    CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA
    CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG
    AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA
    AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA
    GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT
    GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG
    GCGGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT
    GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT
    AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA
    CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC
    CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA
    ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG
    CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC
    CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG
    GAAAAGCCAC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA
    CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG
    GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC
    TCCAGTACCT CTAGCCAGGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCC
    ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT
    GTGGCCGTCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC
    CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC
    GCCAACCAGA TAGGGCTGCC GTCTGCGCAG GTGGCTGCTG CTGAACGGAG AAAGAGAGAA
    GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG
    CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG
    TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA
    CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA
    GACTTGCAGT ACATTACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC
    GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG
    GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG
    AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG
    AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGACGATG ATGTGGACAC CATGCTGATC
    ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGTTGCAGG ACGAGGAGCG GAGGCGGCAG
    CAGCAGTTAG AAGAGATGCG CAAGCGGGAA GCGGAAGACC GAGCGAGGCA AGAGGAAGAG
    CGCCGGCGGC AGGAGGAGGA GCGAACAAAA CGAGACGCTG AAGAAAAGAG GCGACAGGAA
    GAAGGGTATT ACAGCCGCCT GGAAGCCGAG AGGCGCAGAC AGCACGACGA GGCGGCGCGC
    AGGTTGCTGG AGCCCGAGGC GCCCGGTCTG TGCCGCCCTC CGCTTCCCCG GGACTACGAG
    CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC CCTCCTCCCC CGCCTCAGCG AAACGCCTCC
    TACCTCAAAA CACAGGTCCT CTCCCCCGAC TCGCTGTTCA CTGCCAAGTT TGTTGCATAC
    AATGAGGAGG AGGAGGAGGA GGACTGCAGC CTAGCAGGAC CAAACTCTTA CCCAGGATCT
    ACTGGAGCAG CTGTTGGAGC CCATGACGCC TGTCGGGATG CAAAAGAGAA GCGCTCTAAA
    AGCCAAGATG CAGATTCACC TGGAAGTTCT GGGGCCCCTG AAAACTTGAC ATTCAAGGAA
    CGCCAGCGTC TTTTTTCACA AGGTCAAGAT GTATCCAATA AAGTGAAAGC TTCTCGTAAA
    TTAACAGAAC TGGAGAATGA ACTGAACACA AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866368.1
    CDS262..5757
    Translation

    Target ORF information:

    RefSeq Version XM_017010879.1
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010879.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGGAA
    GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA
    AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG
    GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT
    CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT
    CTGCAACGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA
    CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC
    ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT
    TACTGGGGTG CGATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG
    GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT
    ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA
    AATTCATTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT
    ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG
    GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT
    CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA
    CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG
    AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA
    AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA
    GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT
    GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG
    GCGGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT
    GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT
    AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA
    CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC
    CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA
    ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG
    CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC
    CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG
    GAAAAGCCAC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA
    CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG
    GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC
    TCCAGTACCT CTAGCCAGGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCC
    ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT
    GTGGCCGTCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC
    CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC
    GCCAACCAGA TAGGGCTGCC GTCTGCGCAG GTGGCTGCTG CTGAACGGAG AAAGAGAGAA
    GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG
    CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG
    TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA
    CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA
    GACTTGCAGT ACATTACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC
    GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG
    GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG
    AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG
    AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGACGATG ATGTGGACAC CATGCTGATC
    ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGTTGCAGG ACGAGGAGCG GAGGCGGCAG
    CAGCAGTTAG AAGAGATGCG CAAGCGGGAA GCGGAAGACC GAGCGAGGCA AGAGGAAGAG
    CGCCGGCGGC AGGAGGAGGA GCGAACAAAA CGAGACGCTG AAGAAAAGAG GCGACAGGAA
    GAAGGGTATT ACAGCCGCCT GGAAGCCGAG AGGCGCAGAC AGCACGACGA GGCGGCGCGC
    AGGTTGCTGG AGCCCGAGGC GCCCGGTCTG TGCCGCCCTC CGCTTCCCCG GGACTACGAG
    CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC CCTCCTCCCC CGCCTCAGCG AAACGCCTCC
    TACCTCAAAA CACAGGTCCT CTCCCCCGAC TCGCTGTTCA CTGCCAAGTT TGTTGCATAC
    AATGAGGAGG AGGAGGAGGA GGACTGCAGC CTAGCAGGAC CAAACTCTTA CCCAGGATCT
    ACTGGAGCAG CTGTTGGAGC CCATGACGCC TGTCGGGATG CAAAAGAGAA GCGCTCTAAA
    AGCCAAGATG CAGATTCACC TGGAAGTTCT GGGGCCCCTG AAAACTTGAC ATTCAAGGAA
    CGCCAGCGTC TTTTTTCACA AGGTCAAGAT GTATCCAATA AAGTGAAAGC TTCTCGTAAA
    TTAACAGAAC TGGAGAATGA ACTGAACACA AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96617
    Clone ID Related Accession (Same CDS sequence) XM_017010880.1
    Accession Version XM_017010880.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product afadin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGGAA
    GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA
    AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG
    GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT
    CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT
    CTGCAACGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA
    CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC
    ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT
    TACTGGGGTG CGATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG
    GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT
    ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA
    AATTCATTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT
    ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG
    GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT
    CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA
    CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG
    AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA
    AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA
    GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT
    GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG
    GCGGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT
    GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT
    AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA
    CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC
    CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA
    ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG
    CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC
    CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG
    GAAAAGCCAC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA
    CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG
    GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC
    TCCAGTACCT CTAGCCAGGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCC
    ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT
    GTGGCCGTCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC
    CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC
    GCCAACCAGA TAGGGCTGCC GTCTGCGCAG GTGGCTGCTG CTGAACGGAG AAAGAGAGAA
    GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG
    CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG
    TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA
    CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA
    GACTTGCAGT ACATTACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC
    GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG
    GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG
    AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG
    AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGACGATG ATGTGGACAC CATGCTGATC
    ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGTTGCAGG ACGAGGAGCG GAGGCGGCAG
    CAGCAGTTAG AAGAGATGCG CAAGCGGGAA GCGGAAGACC GAGCGAGGCA AGAGGAAGAG
    CGCCGGCGGC AGGAGGAGGA GCGAACAAAA CGAGACGCTG AAGAAAAGAG GCGACAGGAA
    GAAGGGTATT ACAGCCGCCT GGAAGCCGAG AGGCGCAGAC AGCACGACGA GGCGGCGCGC
    AGGTTGCTGG AGCCCGAGGC GCCCGGTCTG TGCCGCCCTC CGCTTCCCCG GGACTACGAG
    CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC CCTCCTCCCC CGCCTCAGCG AAACGCCTCC
    TACCTCAAAA CACAGGTCCT CTCCCCCGAC TCGCTGTTCA CTGCCAAGTT TGTTGCATAC
    AATGAGGAGG AGGAGGAGGA GGACTGCAGC CTAGCAGGAC CAAACTCTTA CCCAGGATCT
    ACTGGAGCAG CTGTTGGAGC CCATGACGCC TGTCGGGATG CAAAAGAGAA GCGCTCTAAA
    AGCCAAGATG CAGATTCACC TGGAAGTTCT GGGGCCCCTG AAAACTTGAC ATTCAAGGAA
    CGCCAGCGTC TTTTTTCACA AGGTCAAGAT GTATCCAATA AAGTGAAAGC TTCTCGTAAA
    TTAACAGAAC TGGAGAATGA ACTGAACACA AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866369.1
    CDS241..5736
    Translation

    Target ORF information:

    RefSeq Version XM_017010880.1
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010880.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC
    CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG
    TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT
    GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC
    ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG
    GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG
    GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT
    CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG
    TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG
    CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG CTTCTGGGCC TGAGCTGATA
    CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA
    AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG
    GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA
    CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGTGT CATCCAGGAA
    GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA
    AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG
    GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT
    CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT
    CTGCAACGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA
    CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC
    ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT
    TACTGGGGTG CGATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG
    GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT
    ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA
    AATTCATTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT
    ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG
    GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT
    CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA
    CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG
    AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA
    AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA
    GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT
    GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG
    GCGGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT
    GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT
    AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA
    CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC
    CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA
    ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG
    CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC
    CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG
    GAAAAGCCAC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA
    CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG
    GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC
    TCCAGTACCT CTAGCCAGGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCC
    ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT
    GTGGCCGTCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC
    CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC
    GCCAACCAGA TAGGGCTGCC GTCTGCGCAG GTGGCTGCTG CTGAACGGAG AAAGAGAGAA
    GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG
    CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG
    TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA
    CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA
    GACTTGCAGT ACATTACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC
    GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG
    GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG
    AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG
    AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGACGATG ATGTGGACAC CATGCTGATC
    ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGTTGCAGG ACGAGGAGCG GAGGCGGCAG
    CAGCAGTTAG AAGAGATGCG CAAGCGGGAA GCGGAAGACC GAGCGAGGCA AGAGGAAGAG
    CGCCGGCGGC AGGAGGAGGA GCGAACAAAA CGAGACGCTG AAGAAAAGAG GCGACAGGAA
    GAAGGGTATT ACAGCCGCCT GGAAGCCGAG AGGCGCAGAC AGCACGACGA GGCGGCGCGC
    AGGTTGCTGG AGCCCGAGGC GCCCGGTCTG TGCCGCCCTC CGCTTCCCCG GGACTACGAG
    CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC CCTCCTCCCC CGCCTCAGCG AAACGCCTCC
    TACCTCAAAA CACAGGTCCT CTCCCCCGAC TCGCTGTTCA CTGCCAAGTT TGTTGCATAC
    AATGAGGAGG AGGAGGAGGA GGACTGCAGC CTAGCAGGAC CAAACTCTTA CCCAGGATCT
    ACTGGAGCAG CTGTTGGAGC CCATGACGCC TGTCGGGATG CAAAAGAGAA GCGCTCTAAA
    AGCCAAGATG CAGATTCACC TGGAAGTTCT GGGGCCCCTG AAAACTTGAC ATTCAAGGAA
    CGCCAGCGTC TTTTTTCACA AGGTCAAGAT GTATCCAATA AAGTGAAAGC TTCTCGTAAA
    TTAACAGAAC TGGAGAATGA ACTGAACACA AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96618
    Clone ID Related Accession (Same CDS sequence) XM_017010881.1
    Accession Version XM_017010881.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product afadin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA
    AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT
    GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG
    GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT
    ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT
    GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT
    TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC
    AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG
    GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTACGAAGA TGGTTCTGAC TCTAGAGATA AGCCAAAGCT TTACCGCCTT
    CAGTTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTGTTT
    GGCCCAGGAA TTCAGCCCCA TCACTGTGAC CTTACCAACA TGGATGGAGT GGTCACTGTG
    ACGCCCAGAA GTATGGACGC AGAAACCTAC GTGGAAGGCC AGCGCATCTC AGAAACCACC
    ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC
    CCCAGTCAGG ATCATGCTCT TGCAAAAAGA TCTGTGGATG GAGGCCTGAT GGTTAAGGGC
    CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTGGGAGG AGATATTCAT
    AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCG
    TCTGCCTCTA GCACAGCCGA GCGGGGAATG GTGAAGCCGA TGATCAGAGT AGAACAGCAG
    CCAGATTATC GCAGGCAAGA AAGCAGAACA CAGGATGCTT CTGGGCCTGA GCTGATACTA
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTGTCTGC CATTATAAAT
    TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA
    TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCTACAGA GCGCACACAT
    AAAGTCATTG CAGTCGTCAA CAAGATGGTG AGCATGATGG AGGGTGTCAT CCAGGAAGTA
    GACCAGGTTG ACCAGAAACA GAAGAATATT GCAGGGGCAC TTGCCTTCTG GATGGCAAAT
    GCATCTGAAC TTCTCAACTT CATTAAGCAA GACCGAGACC TTAGTCGGAT CACACTGGAT
    GCTCAAGATG TTTTAGCACA TTTGGTTCAA ATGGCATTTA AATACTTGGT TCACTGTCTT
    CAATCAGAAC TTAATAATTA CATGCCAGCC TTTCTAGATG ACCCTGAAGA GAACAGTCTG
    CAACGACCAA AAATAGATGA TGTGCTGCAC ACGCTCACAG GAGCCATGTC CTTGCTACGA
    CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTCATC
    AATATGTGGC TGTTCAATAG ATTGGTGACC GACCCAGATT CGGGGCTGTG CTCCCATTAC
    TGGGGTGCGA TTATCCGTCA GCAGTTGGGC CATATTGAAG CCTGGGCTGA GAAGCAGGGG
    CTGGAACTGG CTGCGGACTG TCATCTGAGC AGGATCGTGC AGGCAACGAC TTTGCTTACC
    ATGGATAAGT ATGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT TAAGTTAAAT
    TCATTACAAC TTCAAGCCTT ATTACAGAAC TATCACTGTG CACCTGATGA GCCTTTTATC
    CCAACGGATC TTATAGAAAA TGTAGTGACT GTGGCTGAAA ACACTGCCGA TGAGCTGGCC
    CGCAGTGATG GAAGGGAAGT GCAGTTGGAG GAGGATCCTG ATCTGCAGCT GCCGTTTCTT
    TTGCCAGAAG ATGGTTATTC TTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTCTGTGCCA GAGAGGATTT TGCAGGTTAA TTCCTCACAC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAAGGTGCA GATTATGAAA GTCACCTTCT GCGTGAGAAC
    ACAGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC CCTAAAAAAG
    CAGAATGGAA TGGGCCTTAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTAGGA
    ATCTATGTGA AGTCGGTTGT GAAAGGAGGT GCTGCAGATG TGGATGGACG TCTAGCTGCA
    GGTGATCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTCTCTCA GGAAAGGGCG
    GCAGAACTCA TGACAAGAAC AAGCTCTGTG GTGACACTGG AAGTAGCAAA GCAGGGTGCC
    ATCTACCACG GTCTGGCCAC CCTTCTCAAT CAGCCATCCC CCATGATGCA GAGAATTTCA
    GATCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GCTTTGAGCT CTATAATAAT
    TCAACTCAAA ATGGGTCTCC TGAGAGTCCT CAGCTGCCTT GGGCAGAATA TAGTGAACCA
    AAGAAATTGC CTGGTGATGA CAGACTGATG AAAAATAGAG CTGATCACCG TTCCAGCCCC
    AACGTAGCAA ATCAGCCTCC TAGTCCTGGA GGGAAAAGTG CATATGCCTC TGGAACAACA
    GCGAAGATAA CATCTGTCTC TACTGGAAAC CTCTGCACTG AGGAGCAGAC GCCTCCGCCT
    AGACCTGAAG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT TACCTTCCCA
    GCTTCCAAAT CCCAGGATCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTATGAGGAA
    AAGCCACATA TGCACACAGA TAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACACGT
    TCCCAAGAAG AACTTCGAGA AGATAAAGCT TACCAACTTG AGCGGCATCG AATAGAGGCA
    GCTATGGACC GAAAGTCTGA TAGTGATATG TGGATAAATC AGAGCTCCTC ACTGGACTCC
    AGTACCTCTA GCCAGGAGCA TCTGAACCAT TCCTCTAAGT CGGTCACCCC TGCTTCCACA
    CTGACCAAAA GTGGCCCTGG CCGTTGGAAA ACACCAGCAG CCATACCGGC CACCCCTGTG
    GCCGTCTCCC AGCCAATCCG AACAGACCTG CCTCCGCCAC CCCCGCCACC TCCAGTCCAC
    TATGCCGGTG ATTTCGATGG AATGTCCATG GATTTGCCTC TCCCACCACC CCCTTCCGCC
    AACCAGATAG GGCTGCCGTC TGCGCAGGTG GCTGCTGCTG AACGGAGAAA GAGAGAAGAA
    CATCAGCGTT GGTATGAGAA GGAGAAGGCC CGCCTGGAGG AGGAGCGGGA GAGGAAGCGG
    AGAGAGCAGG AGAGGAAGTT GGGCCAGATG CGCACTCAGT CCTTAAACCC TGCTCCGTTT
    TCTCCCCTGA CTGCACAGCA GATGAAGCCC GAAAAGCCTT CCACACTCCA GCGGCCACAG
    GAAACAGTCA TTCGGGAGCT GCAGCCTCAG CAGCAGCCCC GCACGATCGA GCGCAGAGAC
    TTGCAGTACA TTACAGTCAG CAAAGAGGAG CTTTCCTCGG GGGACAGTCT GTCCCCCGAC
    CCGTGGAAGC GGGACGCCAA GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CATCGTGGAC
    ATGCTGAGCA AGGAGATCCA GGAGCTCCAG AGCAAACCGG ACCGCAGCGC CGAGGAGAGC
    GACCGGCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTCCA GGAGTCGAAG
    CAGAAGGACG AAGATGACGA GGAGGAGGAG GACGATGATG TGGACACCAT GCTGATCATG
    CAGCGCCTGG AGGCTGAACG AAGAGCGAGG TTGCAGGACG AGGAGCGGAG GCGGCAGCAG
    CAGTTAGAAG AGATGCGCAA GCGGGAAGCG GAAGACCGAG CGAGGCAAGA GGAAGAGCGC
    CGGCGGCAGG AGGAGGAGCG AACAAAACGA GACGCTGAAG AAAAGAGGCG ACAGGAAGAA
    GGGTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ACGACGAGGC GGCGCGCAGG
    TTGCTGGAGC CCGAGGCGCC CGGTCTGTGC CGCCCTCCGC TTCCCCGGGA CTACGAGCCC
    CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC
    CTCAAAACAC AGGTCCTCTC CCCCGACTCG CTGTTCACTG CCAAGTTTGT TGCATACAAT
    GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA GCAGGACCAA ACTCTTACCC AGGATCTACT
    GGAGCAGCTG TTGGAGCCCA TGACGCCTGT CGGGATGCAA AAGAGAAGCG CTCTAAAAGC
    CAAGATGCAG ATTCACCTGG AAGTTCTGGG GCCCCTGAAA ACTTGACATT CAAGGAACGC
    CAGCGTCTTT TTTCACAAGG TCAAGATGTA TCCAATAAAG TGAAAGCTTC TCGTAAATTA
    ACAGAACTGG AGAATGAACT GAACACAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866370.1
    CDS245..5737
    Translation

    Target ORF information:

    RefSeq Version XM_017010881.1
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010881.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA
    AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT
    GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG
    GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT
    ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT
    GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT
    TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC
    AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG
    GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTACGAAGA TGGTTCTGAC TCTAGAGATA AGCCAAAGCT TTACCGCCTT
    CAGTTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTGTTT
    GGCCCAGGAA TTCAGCCCCA TCACTGTGAC CTTACCAACA TGGATGGAGT GGTCACTGTG
    ACGCCCAGAA GTATGGACGC AGAAACCTAC GTGGAAGGCC AGCGCATCTC AGAAACCACC
    ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC
    CCCAGTCAGG ATCATGCTCT TGCAAAAAGA TCTGTGGATG GAGGCCTGAT GGTTAAGGGC
    CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTGGGAGG AGATATTCAT
    AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCG
    TCTGCCTCTA GCACAGCCGA GCGGGGAATG GTGAAGCCGA TGATCAGAGT AGAACAGCAG
    CCAGATTATC GCAGGCAAGA AAGCAGAACA CAGGATGCTT CTGGGCCTGA GCTGATACTA
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTGTCTGC CATTATAAAT
    TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA
    TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCTACAGA GCGCACACAT
    AAAGTCATTG CAGTCGTCAA CAAGATGGTG AGCATGATGG AGGGTGTCAT CCAGGAAGTA
    GACCAGGTTG ACCAGAAACA GAAGAATATT GCAGGGGCAC TTGCCTTCTG GATGGCAAAT
    GCATCTGAAC TTCTCAACTT CATTAAGCAA GACCGAGACC TTAGTCGGAT CACACTGGAT
    GCTCAAGATG TTTTAGCACA TTTGGTTCAA ATGGCATTTA AATACTTGGT TCACTGTCTT
    CAATCAGAAC TTAATAATTA CATGCCAGCC TTTCTAGATG ACCCTGAAGA GAACAGTCTG
    CAACGACCAA AAATAGATGA TGTGCTGCAC ACGCTCACAG GAGCCATGTC CTTGCTACGA
    CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTCATC
    AATATGTGGC TGTTCAATAG ATTGGTGACC GACCCAGATT CGGGGCTGTG CTCCCATTAC
    TGGGGTGCGA TTATCCGTCA GCAGTTGGGC CATATTGAAG CCTGGGCTGA GAAGCAGGGG
    CTGGAACTGG CTGCGGACTG TCATCTGAGC AGGATCGTGC AGGCAACGAC TTTGCTTACC
    ATGGATAAGT ATGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT TAAGTTAAAT
    TCATTACAAC TTCAAGCCTT ATTACAGAAC TATCACTGTG CACCTGATGA GCCTTTTATC
    CCAACGGATC TTATAGAAAA TGTAGTGACT GTGGCTGAAA ACACTGCCGA TGAGCTGGCC
    CGCAGTGATG GAAGGGAAGT GCAGTTGGAG GAGGATCCTG ATCTGCAGCT GCCGTTTCTT
    TTGCCAGAAG ATGGTTATTC TTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTCTGTGCCA GAGAGGATTT TGCAGGTTAA TTCCTCACAC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAAGGTGCA GATTATGAAA GTCACCTTCT GCGTGAGAAC
    ACAGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC CCTAAAAAAG
    CAGAATGGAA TGGGCCTTAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTAGGA
    ATCTATGTGA AGTCGGTTGT GAAAGGAGGT GCTGCAGATG TGGATGGACG TCTAGCTGCA
    GGTGATCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTCTCTCA GGAAAGGGCG
    GCAGAACTCA TGACAAGAAC AAGCTCTGTG GTGACACTGG AAGTAGCAAA GCAGGGTGCC
    ATCTACCACG GTCTGGCCAC CCTTCTCAAT CAGCCATCCC CCATGATGCA GAGAATTTCA
    GATCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GCTTTGAGCT CTATAATAAT
    TCAACTCAAA ATGGGTCTCC TGAGAGTCCT CAGCTGCCTT GGGCAGAATA TAGTGAACCA
    AAGAAATTGC CTGGTGATGA CAGACTGATG AAAAATAGAG CTGATCACCG TTCCAGCCCC
    AACGTAGCAA ATCAGCCTCC TAGTCCTGGA GGGAAAAGTG CATATGCCTC TGGAACAACA
    GCGAAGATAA CATCTGTCTC TACTGGAAAC CTCTGCACTG AGGAGCAGAC GCCTCCGCCT
    AGACCTGAAG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT TACCTTCCCA
    GCTTCCAAAT CCCAGGATCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTATGAGGAA
    AAGCCACATA TGCACACAGA TAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACACGT
    TCCCAAGAAG AACTTCGAGA AGATAAAGCT TACCAACTTG AGCGGCATCG AATAGAGGCA
    GCTATGGACC GAAAGTCTGA TAGTGATATG TGGATAAATC AGAGCTCCTC ACTGGACTCC
    AGTACCTCTA GCCAGGAGCA TCTGAACCAT TCCTCTAAGT CGGTCACCCC TGCTTCCACA
    CTGACCAAAA GTGGCCCTGG CCGTTGGAAA ACACCAGCAG CCATACCGGC CACCCCTGTG
    GCCGTCTCCC AGCCAATCCG AACAGACCTG CCTCCGCCAC CCCCGCCACC TCCAGTCCAC
    TATGCCGGTG ATTTCGATGG AATGTCCATG GATTTGCCTC TCCCACCACC CCCTTCCGCC
    AACCAGATAG GGCTGCCGTC TGCGCAGGTG GCTGCTGCTG AACGGAGAAA GAGAGAAGAA
    CATCAGCGTT GGTATGAGAA GGAGAAGGCC CGCCTGGAGG AGGAGCGGGA GAGGAAGCGG
    AGAGAGCAGG AGAGGAAGTT GGGCCAGATG CGCACTCAGT CCTTAAACCC TGCTCCGTTT
    TCTCCCCTGA CTGCACAGCA GATGAAGCCC GAAAAGCCTT CCACACTCCA GCGGCCACAG
    GAAACAGTCA TTCGGGAGCT GCAGCCTCAG CAGCAGCCCC GCACGATCGA GCGCAGAGAC
    TTGCAGTACA TTACAGTCAG CAAAGAGGAG CTTTCCTCGG GGGACAGTCT GTCCCCCGAC
    CCGTGGAAGC GGGACGCCAA GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CATCGTGGAC
    ATGCTGAGCA AGGAGATCCA GGAGCTCCAG AGCAAACCGG ACCGCAGCGC CGAGGAGAGC
    GACCGGCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTCCA GGAGTCGAAG
    CAGAAGGACG AAGATGACGA GGAGGAGGAG GACGATGATG TGGACACCAT GCTGATCATG
    CAGCGCCTGG AGGCTGAACG AAGAGCGAGG TTGCAGGACG AGGAGCGGAG GCGGCAGCAG
    CAGTTAGAAG AGATGCGCAA GCGGGAAGCG GAAGACCGAG CGAGGCAAGA GGAAGAGCGC
    CGGCGGCAGG AGGAGGAGCG AACAAAACGA GACGCTGAAG AAAAGAGGCG ACAGGAAGAA
    GGGTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ACGACGAGGC GGCGCGCAGG
    TTGCTGGAGC CCGAGGCGCC CGGTCTGTGC CGCCCTCCGC TTCCCCGGGA CTACGAGCCC
    CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC
    CTCAAAACAC AGGTCCTCTC CCCCGACTCG CTGTTCACTG CCAAGTTTGT TGCATACAAT
    GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA GCAGGACCAA ACTCTTACCC AGGATCTACT
    GGAGCAGCTG TTGGAGCCCA TGACGCCTGT CGGGATGCAA AAGAGAAGCG CTCTAAAAGC
    CAAGATGCAG ATTCACCTGG AAGTTCTGGG GCCCCTGAAA ACTTGACATT CAAGGAACGC
    CAGCGTCTTT TTTCACAAGG TCAAGATGTA TCCAATAAAG TGAAAGCTTC TCGTAAATTA
    ACAGAACTGG AGAATGAACT GAACACAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96619
    Clone ID Related Accession (Same CDS sequence) XM_017010882.1
    Accession Version XM_017010882.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5478bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product afadin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC
    CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG
    TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC
    ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA
    ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT
    GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT
    AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT
    ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA
    GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA
    CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG
    ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT
    ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT
    ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC
    ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG
    AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC
    AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA
    GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT
    AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAACG ACCAAAAATA
    GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT
    GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC
    AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCGATTATC
    CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG
    GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA
    CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCATT ACAACTTCAA
    GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA
    GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG
    GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT
    TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG
    TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA
    TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG
    CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC
    CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG
    GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC
    AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCGGCAGA ACTCATGACA
    AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG
    GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA
    GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG
    TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT
    GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG
    CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT
    GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC
    CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG
    GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC ACATATGCAC
    ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT
    CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG
    TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAG
    GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CCACACTGAC CAAAAGTGGC
    CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCGT CTCCCAGCCA
    ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC
    GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCGCCAACCA GATAGGGCTG
    CCGTCTGCGC AGGTGGCTGC TGCTGAACGG AGAAAGAGAG AAGAACATCA GCGTTGGTAT
    GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG
    AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA
    CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG
    GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATTACA
    GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC
    GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG
    ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG
    CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT
    GACGAGGAGG AGGAGGACGA TGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT
    GAACGAAGAG CGAGGTTGCA GGACGAGGAG CGGAGGCGGC AGCAGCAGTT AGAAGAGATG
    CGCAAGCGGG AAGCGGAAGA CCGAGCGAGG CAAGAGGAAG AGCGCCGGCG GCAGGAGGAG
    GAGCGAACAA AACGAGACGC TGAAGAAAAG AGGCGACAGG AAGAAGGGTA TTACAGCCGC
    CTGGAAGCCG AGAGGCGCAG ACAGCACGAC GAGGCGGCGC GCAGGTTGCT GGAGCCCGAG
    GCGCCCGGTC TGTGCCGCCC TCCGCTTCCC CGGGACTACG AGCCCCCGTC CCCGTCCCCC
    GCGCCCGGCG CCCCTCCTCC CCCGCCTCAG CGAAACGCCT CCTACCTCAA AACACAGGTC
    CTCTCCCCCG ACTCGCTGTT CACTGCCAAG TTTGTTGCAT ACAATGAGGA GGAGGAGGAG
    GAGGACTGCA GCCTAGCAGG ACCAAACTCT TACCCAGGAT CTACTGGAGC AGCTGTTGGA
    GCCCATGACG CCTGTCGGGA TGCAAAAGAG AAGCGCTCTA AAAGCCAAGA TGCAGATTCA
    CCTGGAAGTT CTGGGGCCCC TGAAAACTTG ACATTCAAGG AACGCCAGCG TCTTTTTTCA
    CAAGGTCAAG ATGTATCCAA TAAAGTGAAA GCTTCTCGTA AATTAACAGA ACTGGAGAAT
    GAACTGAACA CAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866371.1
    CDS257..5734
    Translation

    Target ORF information:

    RefSeq Version XM_017010882.1
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010882.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC
    CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG
    TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC
    ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA
    ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT
    GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT
    AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT
    ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA
    GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA
    CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGCTTCTGG GCCTGAGCTG
    ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT
    ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT
    ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC
    ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG TGTCATCCAG
    AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC
    AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA
    GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT
    AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAACG ACCAAAAATA
    GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT
    GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC
    AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCGATTATC
    CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG
    GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA
    CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCATT ACAACTTCAA
    GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA
    GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG
    GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT
    TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG
    TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA
    TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG
    CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC
    CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG
    GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC
    AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCGGCAGA ACTCATGACA
    AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG
    GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA
    GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG
    TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT
    GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG
    CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT
    GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC
    CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG
    GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC ACATATGCAC
    ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT
    CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG
    TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAG
    GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CCACACTGAC CAAAAGTGGC
    CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCGT CTCCCAGCCA
    ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC
    GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCGCCAACCA GATAGGGCTG
    CCGTCTGCGC AGGTGGCTGC TGCTGAACGG AGAAAGAGAG AAGAACATCA GCGTTGGTAT
    GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG
    AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA
    CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG
    GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATTACA
    GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC
    GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG
    ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG
    CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT
    GACGAGGAGG AGGAGGACGA TGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT
    GAACGAAGAG CGAGGTTGCA GGACGAGGAG CGGAGGCGGC AGCAGCAGTT AGAAGAGATG
    CGCAAGCGGG AAGCGGAAGA CCGAGCGAGG CAAGAGGAAG AGCGCCGGCG GCAGGAGGAG
    GAGCGAACAA AACGAGACGC TGAAGAAAAG AGGCGACAGG AAGAAGGGTA TTACAGCCGC
    CTGGAAGCCG AGAGGCGCAG ACAGCACGAC GAGGCGGCGC GCAGGTTGCT GGAGCCCGAG
    GCGCCCGGTC TGTGCCGCCC TCCGCTTCCC CGGGACTACG AGCCCCCGTC CCCGTCCCCC
    GCGCCCGGCG CCCCTCCTCC CCCGCCTCAG CGAAACGCCT CCTACCTCAA AACACAGGTC
    CTCTCCCCCG ACTCGCTGTT CACTGCCAAG TTTGTTGCAT ACAATGAGGA GGAGGAGGAG
    GAGGACTGCA GCCTAGCAGG ACCAAACTCT TACCCAGGAT CTACTGGAGC AGCTGTTGGA
    GCCCATGACG CCTGTCGGGA TGCAAAAGAG AAGCGCTCTA AAAGCCAAGA TGCAGATTCA
    CCTGGAAGTT CTGGGGCCCC TGAAAACTTG ACATTCAAGG AACGCCAGCG TCTTTTTTCA
    CAAGGTCAAG ATGTATCCAA TAAAGTGAAA GCTTCTCGTA AATTAACAGA ACTGGAGAAT
    GAACTGAACA CAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96620
    Clone ID Related Accession (Same CDS sequence) NM_001366321.2 , XM_017010883.1 , NM_001366321.1
    Accession Version XM_017010883.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5454bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product afadin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT
    AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT
    GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC
    ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC
    CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC
    CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT
    GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT
    GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG
    CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG
    ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT
    TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA
    TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT
    ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC
    AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG
    GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA
    CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC
    CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT
    AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT
    GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA
    GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC
    TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT
    TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA
    GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG
    CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC
    AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT
    GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA
    AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT
    GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC
    ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA
    ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA
    AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA
    ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT
    GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT
    GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT
    GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG
    GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC
    CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA
    GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT
    TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA
    GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT
    GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT
    GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC
    AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC
    CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT
    GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT
    GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT
    CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG
    TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA
    GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA
    CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT
    CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT
    GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG
    GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG
    TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT
    TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC
    CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG
    GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG
    CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG
    GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG
    AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT
    GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC
    GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA
    GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA
    GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG
    CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG
    CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG
    CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT
    GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA
    AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA
    AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA
    AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA
    GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866372.1
    CDS257..5710
    Translation

    Target ORF information:

    RefSeq Version XM_017010883.1
    Organism Homo sapiens(human)
    Definition Homo sapiens myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010883.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA
    GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG
    GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG
    GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT
    GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG
    GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC
    TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA
    AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT
    GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT
    GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG
    GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT
    CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT
    AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT
    GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC
    ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC
    CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC
    CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT
    GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT
    GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG
    CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG
    ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT
    TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA
    TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT
    ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC
    AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG
    GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA
    CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC
    CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT
    AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT
    GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA
    GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC
    TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT
    TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA
    GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG
    CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC
    AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT
    GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA
    AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT
    GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC
    ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA
    ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA
    AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA
    ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT
    GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT
    GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT
    GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG
    GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC
    CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA
    GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT
    TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA
    GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT
    GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT
    GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC
    AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC
    CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT
    GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT
    GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT
    CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG
    TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA
    GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA
    CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT
    CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT
    GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG
    GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG
    TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT
    TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC
    CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG
    GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG
    CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG
    GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG
    AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT
    GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC
    GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA
    GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA
    GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG
    CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG
    CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG
    CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT
    GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA
    AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA
    AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA
    AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA
    GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96621
    Clone ID Related Accession (Same CDS sequence) XM_017010884.1
    Accession Version XM_017010884.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product afadin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGATG GTTCTGACTC TAGAGATAAG
    CCAAAGCTTT ACCGCCTTCA GTTAAGTGTT ACTGAAGTTG GGACAGAAAA GTTGGATGAC
    AACTCTATCC AGTTGTTTGG CCCAGGAATT CAGCCCCATC ACTGTGACCT TACCAACATG
    GATGGAGTGG TCACTGTGAC GCCCAGAAGT ATGGACGCAG AAACCTACGT GGAAGGCCAG
    CGCATCTCAG AAACCACCAT GCTGCAGAGT GGCATGAAAG TGCAGTTTGG GGCGTCCCAT
    GTATTTAAGT TTGTGGACCC CAGTCAGGAT CATGCTCTTG CAAAAAGATC TGTGGATGGA
    GGCCTGATGG TTAAGGGCCC AAGACATAAA CCTGGAATTG TTCAGGAGAC AACTTTTGAT
    TTGGGAGGAG ATATTCATAG TGGGACAGCA TTACCGACAA GCAAGAGCAC CACTAGGCTG
    GACAGCGACA GAGTGTCGTC TGCCTCTAGC ACAGCCGAGC GGGGAATGGT GAAGCCGATG
    ATCAGAGTAG AACAGCAGCC AGATTATCGC AGGCAAGAAA GCAGAACACA GGATGCTTCT
    GGGCCTGAGC TGATACTACC TGCAAGCATT GAATTCAGGG AAAGTTCTGA AGATTCATTT
    TTGTCTGCCA TTATAAATTA TACTAATAGC TCTACAGTCC ACTTTAAGTT GTCCCCTACA
    TATGTATTAT ATATGGCATG CCGGTATGTA TTGTCCAACC AGTACAGACC TGACATCAGC
    CCTACAGAGC GCACACATAA AGTCATTGCA GTCGTCAACA AGATGGTGAG CATGATGGAG
    GGTGTCATCC AGGAAGTAGA CCAGGTTGAC CAGAAACAGA AGAATATTGC AGGGGCACTT
    GCCTTCTGGA TGGCAAATGC ATCTGAACTT CTCAACTTCA TTAAGCAAGA CCGAGACCTT
    AGTCGGATCA CACTGGATGC TCAAGATGTT TTAGCACATT TGGTTCAAAT GGCATTTAAA
    TACTTGGTTC ACTGTCTTCA ATCAGAACTT AATAATTACA TGCCAGCCTT TCTAGATGAC
    CCTGAAGAGA ACAGTCTGCA ACGACCAAAA ATAGATGATG TGCTGCACAC GCTCACAGGA
    GCCATGTCCT TGCTACGACG CTGCAGAGTC AATGCCGCCC TGACCATCCA GCTCTTCTCT
    CAGCTCTTCC ACTTCATCAA TATGTGGCTG TTCAATAGAT TGGTGACCGA CCCAGATTCG
    GGGCTGTGCT CCCATTACTG GGGTGCGATT ATCCGTCAGC AGTTGGGCCA TATTGAAGCC
    TGGGCTGAGA AGCAGGGGCT GGAACTGGCT GCGGACTGTC ATCTGAGCAG GATCGTGCAG
    GCAACGACTT TGCTTACCAT GGATAAGTAT GCACCTGATG ACATTCCAAA TATAAACAGC
    ACCTGCTTTA AGTTAAATTC ATTACAACTT CAAGCCTTAT TACAGAACTA TCACTGTGCA
    CCTGATGAGC CTTTTATCCC AACGGATCTT ATAGAAAATG TAGTGACTGT GGCTGAAAAC
    ACTGCCGATG AGCTGGCCCG CAGTGATGGA AGGGAAGTGC AGTTGGAGGA GGATCCTGAT
    CTGCAGCTGC CGTTTCTTTT GCCAGAAGAT GGTTATTCTT GTGATGTTGT CAGAAACATT
    CCAAATGGTT TACAAGAATT TTTAGACCCT CTGTGCCAGA GAGGATTTTG CAGGTTAATT
    CCTCACACAC GTTCACCAGG TACTTGGACA ATATATTTTG AAGGTGCAGA TTATGAAAGT
    CACCTTCTGC GTGAGAACAC AGAGCTGGCT CAGCCTCTGA GGAAAGAACC TGAAATAATC
    ACTGTGACCC TAAAAAAGCA GAATGGAATG GGCCTTAGCA TTGTTGCAGC AAAGGGTGCT
    GGTCAAGATA AACTAGGAAT CTATGTGAAG TCGGTTGTGA AAGGAGGTGC TGCAGATGTG
    GATGGACGTC TAGCTGCAGG TGATCAGCTC CTCAGTGTGG ATGGACGAAG TCTGGTTGGA
    CTCTCTCAGG AAAGGGCGGC AGAACTCATG ACAAGAACAA GCTCTGTGGT GACACTGGAA
    GTAGCAAAGC AGGGTGCCAT CTACCACGGT CTGGCCACCC TTCTCAATCA GCCATCCCCC
    ATGATGCAGA GAATTTCAGA TCGTCGTGGC TCAGGTAAAC CCCGACCAAA GAGTGAAGGC
    TTTGAGCTCT ATAATAATTC AACTCAAAAT GGGTCTCCTG AGAGTCCTCA GCTGCCTTGG
    GCAGAATATA GTGAACCAAA GAAATTGCCT GGTGATGACA GACTGATGAA AAATAGAGCT
    GATCACCGTT CCAGCCCCAA CGTAGCAAAT CAGCCTCCTA GTCCTGGAGG GAAAAGTGCA
    TATGCCTCTG GAACAACAGC GAAGATAACA TCTGTCTCTA CTGGAAACCT CTGCACTGAG
    GAGCAGACGC CTCCGCCTAG ACCTGAAGCC TACCCCATCC CCACTCAGAC GTACACCAGA
    GAGTATTTTA CCTTCCCAGC TTCCAAATCC CAGGATCGGA TGGCTCCTCC TCAGAACCAG
    TGGCCAAATT ATGAGGAAAA GCCACATATG CACACAGATA GTAATCATTC CAGTATTGCA
    ATTCAGCGTG TTACACGTTC CCAAGAAGAA CTTCGAGAAG ATAAAGCTTA CCAACTTGAG
    CGGCATCGAA TAGAGGCAGC TATGGACCGA AAGTCTGATA GTGATATGTG GATAAATCAG
    AGCTCCTCAC TGGACTCCAG TACCTCTAGC CAGGAGCATC TGAACCATTC CTCTAAGTCG
    GTCACCCCTG CTTCCACACT GACCAAAAGT GGCCCTGGCC GTTGGAAAAC ACCAGCAGCC
    ATACCGGCCA CCCCTGTGGC CGTCTCCCAG CCAATCCGAA CAGACCTGCC TCCGCCACCC
    CCGCCACCTC CAGTCCACTA TGCCGGTGAT TTCGATGGAA TGTCCATGGA TTTGCCTCTC
    CCACCACCCC CTTCCGCCAA CCAGATAGGG CTGCCGTCTG CGCAGGTGGC TGCTGCTGAA
    CGGAGAAAGA GAGAAGAACA TCAGCGTTGG TATGAGAAGG AGAAGGCCCG CCTGGAGGAG
    GAGCGGGAGA GGAAGCGGAG AGAGCAGGAG AGGAAGTTGG GCCAGATGCG CACTCAGTCC
    TTAAACCCTG CTCCGTTTTC TCCCCTGACT GCACAGCAGA TGAAGCCCGA AAAGCCTTCC
    ACACTCCAGC GGCCACAGGA AACAGTCATT CGGGAGCTGC AGCCTCAGCA GCAGCCCCGC
    ACGATCGAGC GCAGAGACTT GCAGTACATT ACAGTCAGCA AAGAGGAGCT TTCCTCGGGG
    GACAGTCTGT CCCCCGACCC GTGGAAGCGG GACGCCAAGG AGAAGCTGGA GAAGCAGCAG
    CAGATGCACA TCGTGGACAT GCTGAGCAAG GAGATCCAGG AGCTCCAGAG CAAACCGGAC
    CGCAGCGCCG AGGAGAGCGA CCGGCTGCGC AAGCTCATGC TGGAGTGGCA GTTCCAGAAG
    AGACTCCAGG AGTCGAAGCA GAAGGACGAA GATGACGAGG AGGAGGAGGA CGATGATGTG
    GACACCATGC TGATCATGCA GCGCCTGGAG GCTGAACGAA GAGCGAGGTT GCAGGACGAG
    GAGCGGAGGC GGCAGCAGCA GTTAGAAGAG ATGCGCAAGC GGGAAGCGGA AGACCGAGCG
    AGGCAAGAGG AAGAGCGCCG GCGGCAGGAG GAGGAGCGAA CAAAACGAGA CGCTGAAGAA
    AAGAGGCGAC AGGAAGAAGG GTATTACAGC CGCCTGGAAG CCGAGAGGCG CAGACAGCAC
    GACGAGGCGG CGCGCAGGTT GCTGGAGCCC GAGGCGCCCG GTCTGTGCCG CCCTCCGCTT
    CCCCGGGACT ACGAGCCCCC GTCCCCGTCC CCCGCGCCCG GCGCCCCTCC TCCCCCGCCT
    CAGCGAAACG CCTCCTACCT CAAAACACAG GTCCTCTCCC CCGACTCGCT GTTCACTGCC
    AAGTTTGTTG CATACAATGA GGAGGAGGAG GAGGAGGACT GCAGCCTAGC AGGACCAAAC
    TCTTACCCAG GATCTACTGG AGCAGCTGTT GGAGCCCATG ACGCCTGTCG GGATGCAAAA
    GAGAAGCGCT CTAAAAGCCA AGATGCAGAT TCACCTGGAA GTTCTGGGGC CCCTGAAAAC
    TTGACATTCA AGGAACGCCA GCGTCTTTTT TCACAAGGTC AAGATGTATC CAATAAAGTG
    AAAGCTTCTC GTAAATTAAC AGAACTGGAG AATGAACTGA ACACAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866373.1
    CDS262..5712
    Translation

    Target ORF information:

    RefSeq Version XM_017010884.1
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT
    CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA
    TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT
    GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG
    GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA
    GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT
    ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA
    GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT
    GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA
    GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT
    CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGATG GTTCTGACTC TAGAGATAAG
    CCAAAGCTTT ACCGCCTTCA GTTAAGTGTT ACTGAAGTTG GGACAGAAAA GTTGGATGAC
    AACTCTATCC AGTTGTTTGG CCCAGGAATT CAGCCCCATC ACTGTGACCT TACCAACATG
    GATGGAGTGG TCACTGTGAC GCCCAGAAGT ATGGACGCAG AAACCTACGT GGAAGGCCAG
    CGCATCTCAG AAACCACCAT GCTGCAGAGT GGCATGAAAG TGCAGTTTGG GGCGTCCCAT
    GTATTTAAGT TTGTGGACCC CAGTCAGGAT CATGCTCTTG CAAAAAGATC TGTGGATGGA
    GGCCTGATGG TTAAGGGCCC AAGACATAAA CCTGGAATTG TTCAGGAGAC AACTTTTGAT
    TTGGGAGGAG ATATTCATAG TGGGACAGCA TTACCGACAA GCAAGAGCAC CACTAGGCTG
    GACAGCGACA GAGTGTCGTC TGCCTCTAGC ACAGCCGAGC GGGGAATGGT GAAGCCGATG
    ATCAGAGTAG AACAGCAGCC AGATTATCGC AGGCAAGAAA GCAGAACACA GGATGCTTCT
    GGGCCTGAGC TGATACTACC TGCAAGCATT GAATTCAGGG AAAGTTCTGA AGATTCATTT
    TTGTCTGCCA TTATAAATTA TACTAATAGC TCTACAGTCC ACTTTAAGTT GTCCCCTACA
    TATGTATTAT ATATGGCATG CCGGTATGTA TTGTCCAACC AGTACAGACC TGACATCAGC
    CCTACAGAGC GCACACATAA AGTCATTGCA GTCGTCAACA AGATGGTGAG CATGATGGAG
    GGTGTCATCC AGGAAGTAGA CCAGGTTGAC CAGAAACAGA AGAATATTGC AGGGGCACTT
    GCCTTCTGGA TGGCAAATGC ATCTGAACTT CTCAACTTCA TTAAGCAAGA CCGAGACCTT
    AGTCGGATCA CACTGGATGC TCAAGATGTT TTAGCACATT TGGTTCAAAT GGCATTTAAA
    TACTTGGTTC ACTGTCTTCA ATCAGAACTT AATAATTACA TGCCAGCCTT TCTAGATGAC
    CCTGAAGAGA ACAGTCTGCA ACGACCAAAA ATAGATGATG TGCTGCACAC GCTCACAGGA
    GCCATGTCCT TGCTACGACG CTGCAGAGTC AATGCCGCCC TGACCATCCA GCTCTTCTCT
    CAGCTCTTCC ACTTCATCAA TATGTGGCTG TTCAATAGAT TGGTGACCGA CCCAGATTCG
    GGGCTGTGCT CCCATTACTG GGGTGCGATT ATCCGTCAGC AGTTGGGCCA TATTGAAGCC
    TGGGCTGAGA AGCAGGGGCT GGAACTGGCT GCGGACTGTC ATCTGAGCAG GATCGTGCAG
    GCAACGACTT TGCTTACCAT GGATAAGTAT GCACCTGATG ACATTCCAAA TATAAACAGC
    ACCTGCTTTA AGTTAAATTC ATTACAACTT CAAGCCTTAT TACAGAACTA TCACTGTGCA
    CCTGATGAGC CTTTTATCCC AACGGATCTT ATAGAAAATG TAGTGACTGT GGCTGAAAAC
    ACTGCCGATG AGCTGGCCCG CAGTGATGGA AGGGAAGTGC AGTTGGAGGA GGATCCTGAT
    CTGCAGCTGC CGTTTCTTTT GCCAGAAGAT GGTTATTCTT GTGATGTTGT CAGAAACATT
    CCAAATGGTT TACAAGAATT TTTAGACCCT CTGTGCCAGA GAGGATTTTG CAGGTTAATT
    CCTCACACAC GTTCACCAGG TACTTGGACA ATATATTTTG AAGGTGCAGA TTATGAAAGT
    CACCTTCTGC GTGAGAACAC AGAGCTGGCT CAGCCTCTGA GGAAAGAACC TGAAATAATC
    ACTGTGACCC TAAAAAAGCA GAATGGAATG GGCCTTAGCA TTGTTGCAGC AAAGGGTGCT
    GGTCAAGATA AACTAGGAAT CTATGTGAAG TCGGTTGTGA AAGGAGGTGC TGCAGATGTG
    GATGGACGTC TAGCTGCAGG TGATCAGCTC CTCAGTGTGG ATGGACGAAG TCTGGTTGGA
    CTCTCTCAGG AAAGGGCGGC AGAACTCATG ACAAGAACAA GCTCTGTGGT GACACTGGAA
    GTAGCAAAGC AGGGTGCCAT CTACCACGGT CTGGCCACCC TTCTCAATCA GCCATCCCCC
    ATGATGCAGA GAATTTCAGA TCGTCGTGGC TCAGGTAAAC CCCGACCAAA GAGTGAAGGC
    TTTGAGCTCT ATAATAATTC AACTCAAAAT GGGTCTCCTG AGAGTCCTCA GCTGCCTTGG
    GCAGAATATA GTGAACCAAA GAAATTGCCT GGTGATGACA GACTGATGAA AAATAGAGCT
    GATCACCGTT CCAGCCCCAA CGTAGCAAAT CAGCCTCCTA GTCCTGGAGG GAAAAGTGCA
    TATGCCTCTG GAACAACAGC GAAGATAACA TCTGTCTCTA CTGGAAACCT CTGCACTGAG
    GAGCAGACGC CTCCGCCTAG ACCTGAAGCC TACCCCATCC CCACTCAGAC GTACACCAGA
    GAGTATTTTA CCTTCCCAGC TTCCAAATCC CAGGATCGGA TGGCTCCTCC TCAGAACCAG
    TGGCCAAATT ATGAGGAAAA GCCACATATG CACACAGATA GTAATCATTC CAGTATTGCA
    ATTCAGCGTG TTACACGTTC CCAAGAAGAA CTTCGAGAAG ATAAAGCTTA CCAACTTGAG
    CGGCATCGAA TAGAGGCAGC TATGGACCGA AAGTCTGATA GTGATATGTG GATAAATCAG
    AGCTCCTCAC TGGACTCCAG TACCTCTAGC CAGGAGCATC TGAACCATTC CTCTAAGTCG
    GTCACCCCTG CTTCCACACT GACCAAAAGT GGCCCTGGCC GTTGGAAAAC ACCAGCAGCC
    ATACCGGCCA CCCCTGTGGC CGTCTCCCAG CCAATCCGAA CAGACCTGCC TCCGCCACCC
    CCGCCACCTC CAGTCCACTA TGCCGGTGAT TTCGATGGAA TGTCCATGGA TTTGCCTCTC
    CCACCACCCC CTTCCGCCAA CCAGATAGGG CTGCCGTCTG CGCAGGTGGC TGCTGCTGAA
    CGGAGAAAGA GAGAAGAACA TCAGCGTTGG TATGAGAAGG AGAAGGCCCG CCTGGAGGAG
    GAGCGGGAGA GGAAGCGGAG AGAGCAGGAG AGGAAGTTGG GCCAGATGCG CACTCAGTCC
    TTAAACCCTG CTCCGTTTTC TCCCCTGACT GCACAGCAGA TGAAGCCCGA AAAGCCTTCC
    ACACTCCAGC GGCCACAGGA AACAGTCATT CGGGAGCTGC AGCCTCAGCA GCAGCCCCGC
    ACGATCGAGC GCAGAGACTT GCAGTACATT ACAGTCAGCA AAGAGGAGCT TTCCTCGGGG
    GACAGTCTGT CCCCCGACCC GTGGAAGCGG GACGCCAAGG AGAAGCTGGA GAAGCAGCAG
    CAGATGCACA TCGTGGACAT GCTGAGCAAG GAGATCCAGG AGCTCCAGAG CAAACCGGAC
    CGCAGCGCCG AGGAGAGCGA CCGGCTGCGC AAGCTCATGC TGGAGTGGCA GTTCCAGAAG
    AGACTCCAGG AGTCGAAGCA GAAGGACGAA GATGACGAGG AGGAGGAGGA CGATGATGTG
    GACACCATGC TGATCATGCA GCGCCTGGAG GCTGAACGAA GAGCGAGGTT GCAGGACGAG
    GAGCGGAGGC GGCAGCAGCA GTTAGAAGAG ATGCGCAAGC GGGAAGCGGA AGACCGAGCG
    AGGCAAGAGG AAGAGCGCCG GCGGCAGGAG GAGGAGCGAA CAAAACGAGA CGCTGAAGAA
    AAGAGGCGAC AGGAAGAAGG GTATTACAGC CGCCTGGAAG CCGAGAGGCG CAGACAGCAC
    GACGAGGCGG CGCGCAGGTT GCTGGAGCCC GAGGCGCCCG GTCTGTGCCG CCCTCCGCTT
    CCCCGGGACT ACGAGCCCCC GTCCCCGTCC CCCGCGCCCG GCGCCCCTCC TCCCCCGCCT
    CAGCGAAACG CCTCCTACCT CAAAACACAG GTCCTCTCCC CCGACTCGCT GTTCACTGCC
    AAGTTTGTTG CATACAATGA GGAGGAGGAG GAGGAGGACT GCAGCCTAGC AGGACCAAAC
    TCTTACCCAG GATCTACTGG AGCAGCTGTT GGAGCCCATG ACGCCTGTCG GGATGCAAAA
    GAGAAGCGCT CTAAAAGCCA AGATGCAGAT TCACCTGGAA GTTCTGGGGC CCCTGAAAAC
    TTGACATTCA AGGAACGCCA GCGTCTTTTT TCACAAGGTC AAGATGTATC CAATAAAGTG
    AAAGCTTCTC GTAAATTAAC AGAACTGGAG AATGAACTGA ACACAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96622
    Clone ID Related Accession (Same CDS sequence) XM_017010885.1
    Accession Version XM_017010885.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product afadin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA
    AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT
    GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG
    GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT
    ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT
    GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT
    TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC
    AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG
    GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA
    AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC
    TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT
    GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC
    ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA
    TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC
    CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG
    GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC
    AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC
    AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGCTTCTGGG
    CCTGAGCTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG
    TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT
    GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT
    ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGT
    GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT
    GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA
    GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA
    GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAACGA
    CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC
    AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG
    TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT
    GCGATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA
    CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT
    AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCATTA
    CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG
    GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT
    GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA
    GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT
    TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG
    CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT
    GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT
    GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT
    CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCGGCAGAA
    CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC
    CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT
    CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT
    CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA
    TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA
    GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG
    ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT
    GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC
    AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCA
    CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA
    GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG
    GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC
    TCTAGCCAGG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC CACACTGACC
    AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCGTC
    TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC
    GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CGCCAACCAG
    ATAGGGCTGC CGTCTGCGCA GGTGGCTGCT GCTGAACGGA GAAAGAGAGA AGAACATCAG
    CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG
    CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC
    CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA
    GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG
    TACATTACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG
    AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG
    AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG
    CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG
    GACGAAGATG ACGAGGAGGA GGAGGACGAT GATGTGGACA CCATGCTGAT CATGCAGCGC
    CTGGAGGCTG AACGAAGAGC GAGGTTGCAG GACGAGGAGC GGAGGCGGCA GCAGCAGTTA
    GAAGAGATGC GCAAGCGGGA AGCGGAAGAC CGAGCGAGGC AAGAGGAAGA GCGCCGGCGG
    CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT
    TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGACG AGGCGGCGCG CAGGTTGCTG
    GAGCCCGAGG CGCCCGGTCT GTGCCGCCCT CCGCTTCCCC GGGACTACGA GCCCCCGTCC
    CCGTCCCCCG CGCCCGGCGC CCCTCCTCCC CCGCCTCAGC GAAACGCCTC CTACCTCAAA
    ACACAGGTCC TCTCCCCCGA CTCGCTGTTC ACTGCCAAGT TTGTTGCATA CAATGAGGAG
    GAGGAGGAGG AGGACTGCAG CCTAGCAGGA CCAAACTCTT ACCCAGGATC TACTGGAGCA
    GCTGTTGGAG CCCATGACGC CTGTCGGGAT GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT
    GCAGATTCAC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAGGA ACGCCAGCGT
    CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA
    CTGGAGAATG AACTGAACAC AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866374.1
    CDS245..5671
    Translation

    Target ORF information:

    RefSeq Version XM_017010885.1
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010885.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT
    GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA
    CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA
    GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT
    GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA
    AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT
    GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG
    GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT
    ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT
    GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT
    TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC
    AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG
    GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA
    AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC
    TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT
    GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC
    ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA
    TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC
    CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG
    GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC
    AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC
    AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGCTTCTGGG
    CCTGAGCTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG
    TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT
    GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT
    ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGT
    GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT
    GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA
    GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA
    GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAACGA
    CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC
    AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG
    TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT
    GCGATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA
    CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT
    AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCATTA
    CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG
    GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT
    GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA
    GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT
    TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG
    CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT
    GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT
    GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT
    CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCGGCAGAA
    CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC
    CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT
    CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT
    CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA
    TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA
    GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG
    ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT
    GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC
    AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCA
    CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA
    GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG
    GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC
    TCTAGCCAGG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC CACACTGACC
    AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCGTC
    TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC
    GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CGCCAACCAG
    ATAGGGCTGC CGTCTGCGCA GGTGGCTGCT GCTGAACGGA GAAAGAGAGA AGAACATCAG
    CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG
    CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC
    CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA
    GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG
    TACATTACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG
    AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG
    AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG
    CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG
    GACGAAGATG ACGAGGAGGA GGAGGACGAT GATGTGGACA CCATGCTGAT CATGCAGCGC
    CTGGAGGCTG AACGAAGAGC GAGGTTGCAG GACGAGGAGC GGAGGCGGCA GCAGCAGTTA
    GAAGAGATGC GCAAGCGGGA AGCGGAAGAC CGAGCGAGGC AAGAGGAAGA GCGCCGGCGG
    CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT
    TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGACG AGGCGGCGCG CAGGTTGCTG
    GAGCCCGAGG CGCCCGGTCT GTGCCGCCCT CCGCTTCCCC GGGACTACGA GCCCCCGTCC
    CCGTCCCCCG CGCCCGGCGC CCCTCCTCCC CCGCCTCAGC GAAACGCCTC CTACCTCAAA
    ACACAGGTCC TCTCCCCCGA CTCGCTGTTC ACTGCCAAGT TTGTTGCATA CAATGAGGAG
    GAGGAGGAGG AGGACTGCAG CCTAGCAGGA CCAAACTCTT ACCCAGGATC TACTGGAGCA
    GCTGTTGGAG CCCATGACGC CTGTCGGGAT GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT
    GCAGATTCAC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAGGA ACGCCAGCGT
    CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA
    CTGGAGAATG AACTGAACAC AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96624
    Clone ID Related Accession (Same CDS sequence) XM_017010887.1
    Accession Version XM_017010887.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product afadin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATCGGGA AGGCAGATTT GTTCTTAAGA ATGAGAATGA CGCCATTCCT CCTAAGTGAA 
    AATTCTCGAC TGGCTGCTGA GGTTTACAAA GACATGCCGG AAACCAGCTT TACTCGAACC
    ATTTCTAATC CTGAGGTGGT TATGAAACGA CGGAGGCAGC AAAAATTGGA AAAGAGAATG
    CAGGAATTTC GGAGCTCAGA TGGGCGGCCT GATTCAGGTG GAACATTGAG AATTTATGCA
    GATAGTTTAA AACCAAATAT TCCCTACAAG ACAATCCTGC TGTCTACTAC AGATCCTGCA
    GACTTTGCTG TGGCTGAAGC TTTAGAGAAG TATGGTCTGG AAAAAGAAAA CCCTAAGGAT
    TACTGCATCG CCCGGGTTAT GCTTCCTCCT GGAGCCCAGC ATTCTGATGA AAAGGGTGCT
    AAAGAAATTA TTCTTGATGA TGATGAGTGT CCTTTACAAA TCTTCAGGGA ATGGCCAAGT
    GACAAAGGGA TTTTAGTCTT TCAGTTGAAG AGGAGGCCAC CAGACCACAT CCCAAAGAAA
    ACCAAGAAAC ACTTGGAAGG CAAGACACCC AAGGGAAAGG AGAGAGCTGA CGGGTCTGGC
    TATGGCTCCA CCCTTCCTCC GGAGAAGCTG CCCTATTTAG TAGAGTTAAG CCCAGGGAGA
    AGGAATCACT TTGCCTACTA CAACTATCAC ACTTACGAAG ATGGTTCTGA CTCTAGAGAT
    AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT
    GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC
    ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC
    CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC
    CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT
    GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT
    GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG
    CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG
    ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT
    TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA
    TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT
    ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC
    AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG
    GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA
    CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC
    CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT
    AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT
    GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA
    GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC
    TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT
    TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA
    GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG
    CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC
    AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT
    GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA
    AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT
    GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC
    ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA
    ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA
    AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA
    ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT
    GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT
    GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT
    GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG
    GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC
    CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA
    GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT
    TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA
    GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT
    GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT
    GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC
    AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC
    CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT
    GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT
    GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT
    CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG
    TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA
    GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA
    CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT
    CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT
    GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG
    GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG
    TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT
    TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC
    CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG
    GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG
    CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG
    GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG
    AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT
    GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC
    GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA
    GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA
    GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG
    CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG
    CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG
    CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT
    GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA
    AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA
    AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA
    AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA
    GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866376.1
    CDS21..4994
    Translation

    Target ORF information:

    RefSeq Version XM_017010887.1
    Organism Homo sapiens(human)
    Definition Homo sapiens afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010887.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATCGGGA AGGCAGATTT GTTCTTAAGA ATGAGAATGA CGCCATTCCT CCTAAGTGAA 
    AATTCTCGAC TGGCTGCTGA GGTTTACAAA GACATGCCGG AAACCAGCTT TACTCGAACC
    ATTTCTAATC CTGAGGTGGT TATGAAACGA CGGAGGCAGC AAAAATTGGA AAAGAGAATG
    CAGGAATTTC GGAGCTCAGA TGGGCGGCCT GATTCAGGTG GAACATTGAG AATTTATGCA
    GATAGTTTAA AACCAAATAT TCCCTACAAG ACAATCCTGC TGTCTACTAC AGATCCTGCA
    GACTTTGCTG TGGCTGAAGC TTTAGAGAAG TATGGTCTGG AAAAAGAAAA CCCTAAGGAT
    TACTGCATCG CCCGGGTTAT GCTTCCTCCT GGAGCCCAGC ATTCTGATGA AAAGGGTGCT
    AAAGAAATTA TTCTTGATGA TGATGAGTGT CCTTTACAAA TCTTCAGGGA ATGGCCAAGT
    GACAAAGGGA TTTTAGTCTT TCAGTTGAAG AGGAGGCCAC CAGACCACAT CCCAAAGAAA
    ACCAAGAAAC ACTTGGAAGG CAAGACACCC AAGGGAAAGG AGAGAGCTGA CGGGTCTGGC
    TATGGCTCCA CCCTTCCTCC GGAGAAGCTG CCCTATTTAG TAGAGTTAAG CCCAGGGAGA
    AGGAATCACT TTGCCTACTA CAACTATCAC ACTTACGAAG ATGGTTCTGA CTCTAGAGAT
    AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT
    GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC
    ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC
    CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC
    CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT
    GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT
    GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG
    CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG
    ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGCT
    TCTGGGCCTG AGCTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA
    TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT
    ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC
    AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG
    GAGGGTGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA
    CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC
    CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT
    AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT
    GACCCTGAAG AGAACAGTCT GCAACGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA
    GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC
    TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT
    TCGGGGCTGT GCTCCCATTA CTGGGGTGCG ATTATCCGTC AGCAGTTGGG CCATATTGAA
    GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG
    CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC
    AGCACCTGCT TTAAGTTAAA TTCATTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT
    GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA
    AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT
    GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC
    ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA
    ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA
    AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA
    ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT
    GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT
    GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT
    GGACTCTCTC AGGAAAGGGC GGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG
    GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC
    CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA
    GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT
    TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA
    GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT
    GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT
    GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC
    AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC
    CAGTGGCCAA ATTATGAGGA AAAGCCACAT ATGCACACAG ATAGTAATCA TTCCAGTATT
    GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT
    GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT
    CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAGGAGC ATCTGAACCA TTCCTCTAAG
    TCGGTCACCC CTGCTTCCAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA
    GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA
    CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT
    CTCCCACCAC CCCCTTCCGC CAACCAGATA GGGCTGCCGT CTGCGCAGGT GGCTGCTGCT
    GAACGGAGAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG
    GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG
    TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT
    TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC
    CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATTACAGTCA GCAAAGAGGA GCTTTCCTCG
    GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG
    CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG
    GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG
    AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGACGATGAT
    GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GTTGCAGGAC
    GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA
    GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA
    GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG
    CACGACGAGG CGGCGCGCAG GTTGCTGGAG CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG
    CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG
    CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT
    GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGACCA
    AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGGGATGCA
    AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA
    AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA
    GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.