ME2 cDNA ORF clone, Homo sapiens(human)

The following ME2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ME2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu21107 NM_002396.5
Latest version!
Homo sapiens malic enzyme 2 (ME2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$244.50-$342.30
$489.00
OHu12192 NM_001168335.2
Latest version!
Homo sapiens malic enzyme 2 (ME2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
View Old Accession Versions >>
OHu12192 NM_001168335.1 Homo sapiens malic enzyme 2 (ME2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OHu21107 NM_002396.4 Homo sapiens malic enzyme 2 (ME2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$244.50-$342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu21107
    Clone ID Related Accession (Same CDS sequence) NM_002396.4 , NM_002396.5
    Accession Version NM_002396.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-26
    Organism Homo sapiens(human)
    Product NAD-dependent malic enzyme, mitochondrial isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA644731.1, BI559761.1, M55905.1, AK313391.1, AC087687.5 and CD370709.1. This sequence is a reference standard in the RefSeqGene project. On Dec 4, 2009 this sequence version replaced NM_002396.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.255124.1, SRR1660807.120905.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTTGTCCC GGTTAAGAGT AGTTTCCACC ACTTGTACTT TGGCATGTCG ACATTTGCAC 
    ATAAAAGAAA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG AATGGCATTT
    ACTTTACAAG AACGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA
    CAAGATATTC AAGCCTTACG ATTTCATAGA AACTTGAAGA AAATGACTAG CCCTTTGGAA
    AAATATATCT ACATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA TAGAATACTG
    CAAGATGACA TTGAGAGTTT AATGCCAATT GTATATACAC CGACGGTTGG TCTTGCCTGC
    TCCCAGTATG GACACATCTT TAGAAGACCT AAGGGATTAT TTATTTCGAT CTCAGACAGA
    GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTTGTAGTG
    ACTGATGGAG AGAGAATTCT GGGTCTTGGA GATCTGGGTG TCTATGGAAT GGGAATTCCA
    GTAGGAAAAC TTTGTTTGTA TACAGCTTGT GCAGGAATAC GGCCTGATAG ATGCCTGCCA
    GTGTGTATTG ATGTGGGAAC TGATAATATC GCACTCTTAA AAGACCCATT TTACATGGGC
    TTGTACCAGA AACGAGATCG CACACAACAG TATGATGACC TGATTGATGA GTTTATGAAA
    GCTATTACTG ACAGATATGG CCGGAACACA CTCATTCAGT TCGAAGACTT TGGAAATCAT
    AATGCATTCA GGTTCTTGAG AAAGTACCGA GAAAAATATT GTACTTTCAA TGATGATATT
    CAAGGGACAG CTGCAGTAGC TCTAGCAGGT CTTCTTGCAG CACAAAAAGT TATTAGTAAA
    CCAATCTCCG AACACAAAAT CTTATTCCTT GGAGCAGGAG AGGCTGCTCT TGGAATTGCA
    AATCTTATAG TTATGTCTAT GGTAGAAAAT GGCCTGTCAG AACAAGAGGC ACAAAAGAAA
    ATCTGGATGT TTGACAAGTA TGGTTTATTA GTTAAGGGAC GGAAAGCAAA AATAGATAGT
    TATCAGGAAC CATTTACTCA CTCAGCCCCA GAGAGCATAC CTGATACTTT TGAAGATGCA
    GTGAATATAC TGAAGCCTTC AACTATAATT GGAGTTGCAG GTGCTGGCCG TCTTTTCACT
    CCTGATGTAA TCAGAGCCAT GGCCTCTATC AATGAAAGGC CTGTAATATT TGCATTAAGT
    AATCCTACAG CACAGGCAGA GTGCACGGCT GAAGAAGCAT ATACACTTAC AGAGGGCAGG
    TGTTTGTTTG CCAGTGGCAG TCCATTTGGG CCAGTGAAAC TTACAGATGG GCGAGTCTTT
    ACACCAGGTC AAGGAAACAA TGTTTATATT TTTCCAGGTG TGGCTTTAGC TGTTATTCTC
    TGTAACACCC GGCATATTAG TGACAGTGTT TTCCTAGAAG CTGCAAAGGC CCTGACAAGC
    CAATTGACAG ATGAAGAGCT AGCCCAAGGG AGACTTTACC CACCGCTTGC TAATATTCAG
    GAAGTTTCTA TTAACATTGC TATTAAAGTT ACAGAATACC TATATGCTAA TAAAATGGCT
    TTCCGATACC CAGAACCTGA AGACAAGGCC AAATATGTTA AAGAAAGAAC ATGGCGGAGT
    GAATATGATT CCCTGCTGCC AGATGTGTAT GAATGGCCAG AATCTGCATC AAGCCCTCCT
    GTGATAACAG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002387.1
    CDS260..2014
    Translation

    Target ORF information:

    RefSeq Version NM_002396.4
    Organism Homo sapiens(human)
    Definition Homo sapiens malic enzyme 2 (ME2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002396.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTTGTCCC GGTTAAGAGT AGTTTCCACC ACTTGTACTT TGGCATGTCG ACATTTGCAC 
    ATAAAAGAAA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG AATGGCATTT
    ACTTTACAAG AACGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA
    CAAGATATTC AAGCCTTACG ATTTCATAGA AACTTGAAGA AAATGACTAG CCCTTTGGAA
    AAATATATCT ACATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA TAGAATACTG
    CAAGATGACA TTGAGAGTTT AATGCCAATT GTATATACAC CGACGGTTGG TCTTGCCTGC
    TCCCAGTATG GACACATCTT TAGAAGACCT AAGGGATTAT TTATTTCGAT CTCAGACAGA
    GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTTGTAGTG
    ACTGATGGAG AGAGAATTCT GGGTCTTGGA GATCTGGGTG TCTATGGAAT GGGAATTCCA
    GTAGGAAAAC TTTGTTTGTA TACAGCTTGT GCAGGAATAC GGCCTGATAG ATGCCTGCCA
    GTGTGTATTG ATGTGGGAAC TGATAATATC GCACTCTTAA AAGACCCATT TTACATGGGC
    TTGTACCAGA AACGAGATCG CACACAACAG TATGATGACC TGATTGATGA GTTTATGAAA
    GCTATTACTG ACAGATATGG CCGGAACACA CTCATTCAGT TCGAAGACTT TGGAAATCAT
    AATGCATTCA GGTTCTTGAG AAAGTACCGA GAAAAATATT GTACTTTCAA TGATGATATT
    CAAGGGACAG CTGCAGTAGC TCTAGCAGGT CTTCTTGCAG CACAAAAAGT TATTAGTAAA
    CCAATCTCCG AACACAAAAT CTTATTCCTT GGAGCAGGAG AGGCTGCTCT TGGAATTGCA
    AATCTTATAG TTATGTCTAT GGTAGAAAAT GGCCTGTCAG AACAAGAGGC ACAAAAGAAA
    ATCTGGATGT TTGACAAGTA TGGTTTATTA GTTAAGGGAC GGAAAGCAAA AATAGATAGT
    TATCAGGAAC CATTTACTCA CTCAGCCCCA GAGAGCATAC CTGATACTTT TGAAGATGCA
    GTGAATATAC TGAAGCCTTC AACTATAATT GGAGTTGCAG GTGCTGGCCG TCTTTTCACT
    CCTGATGTAA TCAGAGCCAT GGCCTCTATC AATGAAAGGC CTGTAATATT TGCATTAAGT
    AATCCTACAG CACAGGCAGA GTGCACGGCT GAAGAAGCAT ATACACTTAC AGAGGGCAGG
    TGTTTGTTTG CCAGTGGCAG TCCATTTGGG CCAGTGAAAC TTACAGATGG GCGAGTCTTT
    ACACCAGGTC AAGGAAACAA TGTTTATATT TTTCCAGGTG TGGCTTTAGC TGTTATTCTC
    TGTAACACCC GGCATATTAG TGACAGTGTT TTCCTAGAAG CTGCAAAGGC CCTGACAAGC
    CAATTGACAG ATGAAGAGCT AGCCCAAGGG AGACTTTACC CACCGCTTGC TAATATTCAG
    GAAGTTTCTA TTAACATTGC TATTAAAGTT ACAGAATACC TATATGCTAA TAAAATGGCT
    TTCCGATACC CAGAACCTGA AGACAAGGCC AAATATGTTA AAGAAAGAAC ATGGCGGAGT
    GAATATGATT CCCTGCTGCC AGATGTGTAT GAATGGCCAG AATCTGCATC AAGCCCTCCT
    GTGATAACAG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21107
    Clone ID Related Accession (Same CDS sequence) NM_002396.4 , NM_002396.5
    Accession Version NM_002396.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product NAD-dependent malic enzyme, mitochondrial isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI559761.1, M55905.1, AK313391.1, AC087687.5 and CD370709.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_002396.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.6818.1, SRR1660807.255124.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000321341.11/ ENSP00000321070.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTTGTCCC GGTTAAGAGT AGTTTCCACC ACTTGTACTT TGGCATGTCG ACATTTGCAC 
    ATAAAAGAAA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG AATGGCATTT
    ACTTTACAAG AACGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA
    CAAGATATTC AAGCCTTACG ATTTCATAGA AACTTGAAGA AAATGACTAG CCCTTTGGAA
    AAATATATCT ACATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA TAGAATACTG
    CAAGATGACA TTGAGAGTTT AATGCCAATT GTATATACAC CGACGGTTGG TCTTGCCTGC
    TCCCAGTATG GACACATCTT TAGAAGACCT AAGGGATTAT TTATTTCGAT CTCAGACAGA
    GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTTGTAGTG
    ACTGATGGAG AGAGAATTCT GGGTCTTGGA GATCTGGGTG TCTATGGAAT GGGAATTCCA
    GTAGGAAAAC TTTGTTTGTA TACAGCTTGT GCAGGAATAC GGCCTGATAG ATGCCTGCCA
    GTGTGTATTG ATGTGGGAAC TGATAATATC GCACTCTTAA AAGACCCATT TTACATGGGC
    TTGTACCAGA AACGAGATCG CACACAACAG TATGATGACC TGATTGATGA GTTTATGAAA
    GCTATTACTG ACAGATATGG CCGGAACACA CTCATTCAGT TCGAAGACTT TGGAAATCAT
    AATGCATTCA GGTTCTTGAG AAAGTACCGA GAAAAATATT GTACTTTCAA TGATGATATT
    CAAGGGACAG CTGCAGTAGC TCTAGCAGGT CTTCTTGCAG CACAAAAAGT TATTAGTAAA
    CCAATCTCCG AACACAAAAT CTTATTCCTT GGAGCAGGAG AGGCTGCTCT TGGAATTGCA
    AATCTTATAG TTATGTCTAT GGTAGAAAAT GGCCTGTCAG AACAAGAGGC ACAAAAGAAA
    ATCTGGATGT TTGACAAGTA TGGTTTATTA GTTAAGGGAC GGAAAGCAAA AATAGATAGT
    TATCAGGAAC CATTTACTCA CTCAGCCCCA GAGAGCATAC CTGATACTTT TGAAGATGCA
    GTGAATATAC TGAAGCCTTC AACTATAATT GGAGTTGCAG GTGCTGGCCG TCTTTTCACT
    CCTGATGTAA TCAGAGCCAT GGCCTCTATC AATGAAAGGC CTGTAATATT TGCATTAAGT
    AATCCTACAG CACAGGCAGA GTGCACGGCT GAAGAAGCAT ATACACTTAC AGAGGGCAGG
    TGTTTGTTTG CCAGTGGCAG TCCATTTGGG CCAGTGAAAC TTACAGATGG GCGAGTCTTT
    ACACCAGGTC AAGGAAACAA TGTTTATATT TTTCCAGGTG TGGCTTTAGC TGTTATTCTC
    TGTAACACCC GGCATATTAG TGACAGTGTT TTCCTAGAAG CTGCAAAGGC CCTGACAAGC
    CAATTGACAG ATGAAGAGCT AGCCCAAGGG AGACTTTACC CACCGCTTGC TAATATTCAG
    GAAGTTTCTA TTAACATTGC TATTAAAGTT ACAGAATACC TATATGCTAA TAAAATGGCT
    TTCCGATACC CAGAACCTGA AGACAAGGCC AAATATGTTA AAGAAAGAAC ATGGCGGAGT
    GAATATGATT CCCTGCTGCC AGATGTGTAT GAATGGCCAG AATCTGCATC AAGCCCTCCT
    GTGATAACAG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002387.1
    CDS204..1958
    Translation

    Target ORF information:

    RefSeq Version NM_002396.5
    Organism Homo sapiens(human)
    Definition Homo sapiens malic enzyme 2 (ME2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002396.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTTGTCCC GGTTAAGAGT AGTTTCCACC ACTTGTACTT TGGCATGTCG ACATTTGCAC 
    ATAAAAGAAA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG AATGGCATTT
    ACTTTACAAG AACGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA
    CAAGATATTC AAGCCTTACG ATTTCATAGA AACTTGAAGA AAATGACTAG CCCTTTGGAA
    AAATATATCT ACATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA TAGAATACTG
    CAAGATGACA TTGAGAGTTT AATGCCAATT GTATATACAC CGACGGTTGG TCTTGCCTGC
    TCCCAGTATG GACACATCTT TAGAAGACCT AAGGGATTAT TTATTTCGAT CTCAGACAGA
    GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTTGTAGTG
    ACTGATGGAG AGAGAATTCT GGGTCTTGGA GATCTGGGTG TCTATGGAAT GGGAATTCCA
    GTAGGAAAAC TTTGTTTGTA TACAGCTTGT GCAGGAATAC GGCCTGATAG ATGCCTGCCA
    GTGTGTATTG ATGTGGGAAC TGATAATATC GCACTCTTAA AAGACCCATT TTACATGGGC
    TTGTACCAGA AACGAGATCG CACACAACAG TATGATGACC TGATTGATGA GTTTATGAAA
    GCTATTACTG ACAGATATGG CCGGAACACA CTCATTCAGT TCGAAGACTT TGGAAATCAT
    AATGCATTCA GGTTCTTGAG AAAGTACCGA GAAAAATATT GTACTTTCAA TGATGATATT
    CAAGGGACAG CTGCAGTAGC TCTAGCAGGT CTTCTTGCAG CACAAAAAGT TATTAGTAAA
    CCAATCTCCG AACACAAAAT CTTATTCCTT GGAGCAGGAG AGGCTGCTCT TGGAATTGCA
    AATCTTATAG TTATGTCTAT GGTAGAAAAT GGCCTGTCAG AACAAGAGGC ACAAAAGAAA
    ATCTGGATGT TTGACAAGTA TGGTTTATTA GTTAAGGGAC GGAAAGCAAA AATAGATAGT
    TATCAGGAAC CATTTACTCA CTCAGCCCCA GAGAGCATAC CTGATACTTT TGAAGATGCA
    GTGAATATAC TGAAGCCTTC AACTATAATT GGAGTTGCAG GTGCTGGCCG TCTTTTCACT
    CCTGATGTAA TCAGAGCCAT GGCCTCTATC AATGAAAGGC CTGTAATATT TGCATTAAGT
    AATCCTACAG CACAGGCAGA GTGCACGGCT GAAGAAGCAT ATACACTTAC AGAGGGCAGG
    TGTTTGTTTG CCAGTGGCAG TCCATTTGGG CCAGTGAAAC TTACAGATGG GCGAGTCTTT
    ACACCAGGTC AAGGAAACAA TGTTTATATT TTTCCAGGTG TGGCTTTAGC TGTTATTCTC
    TGTAACACCC GGCATATTAG TGACAGTGTT TTCCTAGAAG CTGCAAAGGC CCTGACAAGC
    CAATTGACAG ATGAAGAGCT AGCCCAAGGG AGACTTTACC CACCGCTTGC TAATATTCAG
    GAAGTTTCTA TTAACATTGC TATTAAAGTT ACAGAATACC TATATGCTAA TAAAATGGCT
    TTCCGATACC CAGAACCTGA AGACAAGGCC AAATATGTTA AAGAAAGAAC ATGGCGGAGT
    GAATATGATT CCCTGCTGCC AGATGTGTAT GAATGGCCAG AATCTGCATC AAGCCCTCCT
    GTGATAACAG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12192
    Clone ID Related Accession (Same CDS sequence) NM_001168335.1 , NM_001168335.2
    Accession Version NM_001168335.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1440bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product NAD-dependent malic enzyme, mitochondrial isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA644731.1, BI465083.1, BC000147.1, AC087687.5 and CD370709.1. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000147.1, SRR1803617.98022.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTTGTCCC GGTTAAGAGT AGTTTCCACC ACTTGTACTT TGGCATGTCG ACATTTGCAC 
    ATAAAAGAAA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG AATGGCATTT
    ACTTTACAAG AACGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA
    CAAGATATTC AAGCCTTACG ATTTCATAGA AACTTGAAGA AAATGACTAG CCCTTTGGAA
    AAATATATCT ACATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA TAGAATACTG
    CAAGATGACA TTGAGAGTTT AATGCCAATT GTATATACAC CGACGGTTGG TCTTGCCTGC
    TCCCAGTATG GACACATCTT TAGAAGACCT AAGGGATTAT TTATTTCGAT CTCAGACAGA
    GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTTGTAGTG
    ACTGATGGAG AGAGAATTCT GGGTCTTGGA GATCTGGGTG TCTATGGAAT GGGAATTCCA
    GTAGGAAAAC TTTGTTTGTA TACAGCTTGT GCAGGAATAC GGCCTGATAG ATGCCTGCCA
    GTGTGTATTG ATGTGGGAAC TGATAATATC GCACTCTTAA AAGACCCATT TTACATGGGC
    TTGTACCAGA AACGAGATCG CACACAACAG TATGATGACC TGATTGATGA GTTTATGAAA
    GCTATTACTG ACAGATATGG CCGGAACACA CTCATTCAGT TCGAAGACTT TGGAAATCAT
    AATGCATTCA GGTTCTTGAG AAAGTACCGA GAAAAATATT GTACTTTCAA TGATGATATT
    CAAGGGACAG CTGCAGTAGC TCTAGCAGGT CTTCTTGCAG CACAAAAAGT TATTAGTAAA
    CCAATCTCCG AACACAAAAT CTTATTCCTT GGAGCAGGAG AGGCTGCTCT TGGAATTGCA
    AATCTTATAG TTATGTCTAT GGTAGAAAAT GGCCTGTCAG AACAAGAGGC ACAAAAGAAA
    ATCTGGATGT TTGACAAGTA TGGTTTATTA GTTAAGGGAC GGAAAGCAAA AATAGATAGT
    TATCAGGAAC CATTTACTCA CTCAGCCCCA GAGAGCATAC CTGATACTTT TGAAGATGCA
    GTGAATATAC TGAAGCCTTC AACTATAATT GGAGTTGCAG GTGCTGGCCG TCTTTTCACT
    CCTGATGTAA TCAGAGCCAT GGCCTCTATC AATGAAAGGC CTGTAATATT TGCATTAAGT
    AATCCTACAG CACAGGCAGA GTGCACGGCT GAAGAAGCAT ATACACTTAC AGAGGGCAGG
    TGTTTGTTTG CCAGTGGCAG TCCATTTGGG CCAGTGAAAC TTACAGATGG GCGAGTCTTT
    ACACCAGGTC AAGGAAACAA TGTTTATATT TTTCCAGGTT ACAGAATACC TATATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001161807.1
    CDS260..1699
    Translation

    Target ORF information:

    RefSeq Version NM_001168335.1
    Organism Homo sapiens(human)
    Definition Homo sapiens malic enzyme 2 (ME2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001168335.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTTGTCCC GGTTAAGAGT AGTTTCCACC ACTTGTACTT TGGCATGTCG ACATTTGCAC 
    ATAAAAGAAA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG AATGGCATTT
    ACTTTACAAG AACGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA
    CAAGATATTC AAGCCTTACG ATTTCATAGA AACTTGAAGA AAATGACTAG CCCTTTGGAA
    AAATATATCT ACATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA TAGAATACTG
    CAAGATGACA TTGAGAGTTT AATGCCAATT GTATATACAC CGACGGTTGG TCTTGCCTGC
    TCCCAGTATG GACACATCTT TAGAAGACCT AAGGGATTAT TTATTTCGAT CTCAGACAGA
    GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTTGTAGTG
    ACTGATGGAG AGAGAATTCT GGGTCTTGGA GATCTGGGTG TCTATGGAAT GGGAATTCCA
    GTAGGAAAAC TTTGTTTGTA TACAGCTTGT GCAGGAATAC GGCCTGATAG ATGCCTGCCA
    GTGTGTATTG ATGTGGGAAC TGATAATATC GCACTCTTAA AAGACCCATT TTACATGGGC
    TTGTACCAGA AACGAGATCG CACACAACAG TATGATGACC TGATTGATGA GTTTATGAAA
    GCTATTACTG ACAGATATGG CCGGAACACA CTCATTCAGT TCGAAGACTT TGGAAATCAT
    AATGCATTCA GGTTCTTGAG AAAGTACCGA GAAAAATATT GTACTTTCAA TGATGATATT
    CAAGGGACAG CTGCAGTAGC TCTAGCAGGT CTTCTTGCAG CACAAAAAGT TATTAGTAAA
    CCAATCTCCG AACACAAAAT CTTATTCCTT GGAGCAGGAG AGGCTGCTCT TGGAATTGCA
    AATCTTATAG TTATGTCTAT GGTAGAAAAT GGCCTGTCAG AACAAGAGGC ACAAAAGAAA
    ATCTGGATGT TTGACAAGTA TGGTTTATTA GTTAAGGGAC GGAAAGCAAA AATAGATAGT
    TATCAGGAAC CATTTACTCA CTCAGCCCCA GAGAGCATAC CTGATACTTT TGAAGATGCA
    GTGAATATAC TGAAGCCTTC AACTATAATT GGAGTTGCAG GTGCTGGCCG TCTTTTCACT
    CCTGATGTAA TCAGAGCCAT GGCCTCTATC AATGAAAGGC CTGTAATATT TGCATTAAGT
    AATCCTACAG CACAGGCAGA GTGCACGGCT GAAGAAGCAT ATACACTTAC AGAGGGCAGG
    TGTTTGTTTG CCAGTGGCAG TCCATTTGGG CCAGTGAAAC TTACAGATGG GCGAGTCTTT
    ACACCAGGTC AAGGAAACAA TGTTTATATT TTTCCAGGTT ACAGAATACC TATATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12192
    Clone ID Related Accession (Same CDS sequence) NM_001168335.1 , NM_001168335.2
    Accession Version NM_001168335.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1440bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product NAD-dependent malic enzyme, mitochondrial isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI465083.1, BC000147.1, AC087687.5 and CD370709.1. On May 31, 2019 this sequence version replaced NM_001168335.1. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000147.1, SRR1803617.98022.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTTGTCCC GGTTAAGAGT AGTTTCCACC ACTTGTACTT TGGCATGTCG ACATTTGCAC 
    ATAAAAGAAA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG AATGGCATTT
    ACTTTACAAG AACGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA
    CAAGATATTC AAGCCTTACG ATTTCATAGA AACTTGAAGA AAATGACTAG CCCTTTGGAA
    AAATATATCT ACATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA TAGAATACTG
    CAAGATGACA TTGAGAGTTT AATGCCAATT GTATATACAC CGACGGTTGG TCTTGCCTGC
    TCCCAGTATG GACACATCTT TAGAAGACCT AAGGGATTAT TTATTTCGAT CTCAGACAGA
    GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTTGTAGTG
    ACTGATGGAG AGAGAATTCT GGGTCTTGGA GATCTGGGTG TCTATGGAAT GGGAATTCCA
    GTAGGAAAAC TTTGTTTGTA TACAGCTTGT GCAGGAATAC GGCCTGATAG ATGCCTGCCA
    GTGTGTATTG ATGTGGGAAC TGATAATATC GCACTCTTAA AAGACCCATT TTACATGGGC
    TTGTACCAGA AACGAGATCG CACACAACAG TATGATGACC TGATTGATGA GTTTATGAAA
    GCTATTACTG ACAGATATGG CCGGAACACA CTCATTCAGT TCGAAGACTT TGGAAATCAT
    AATGCATTCA GGTTCTTGAG AAAGTACCGA GAAAAATATT GTACTTTCAA TGATGATATT
    CAAGGGACAG CTGCAGTAGC TCTAGCAGGT CTTCTTGCAG CACAAAAAGT TATTAGTAAA
    CCAATCTCCG AACACAAAAT CTTATTCCTT GGAGCAGGAG AGGCTGCTCT TGGAATTGCA
    AATCTTATAG TTATGTCTAT GGTAGAAAAT GGCCTGTCAG AACAAGAGGC ACAAAAGAAA
    ATCTGGATGT TTGACAAGTA TGGTTTATTA GTTAAGGGAC GGAAAGCAAA AATAGATAGT
    TATCAGGAAC CATTTACTCA CTCAGCCCCA GAGAGCATAC CTGATACTTT TGAAGATGCA
    GTGAATATAC TGAAGCCTTC AACTATAATT GGAGTTGCAG GTGCTGGCCG TCTTTTCACT
    CCTGATGTAA TCAGAGCCAT GGCCTCTATC AATGAAAGGC CTGTAATATT TGCATTAAGT
    AATCCTACAG CACAGGCAGA GTGCACGGCT GAAGAAGCAT ATACACTTAC AGAGGGCAGG
    TGTTTGTTTG CCAGTGGCAG TCCATTTGGG CCAGTGAAAC TTACAGATGG GCGAGTCTTT
    ACACCAGGTC AAGGAAACAA TGTTTATATT TTTCCAGGTT ACAGAATACC TATATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001161807.1
    CDS204..1643
    Translation

    Target ORF information:

    RefSeq Version NM_001168335.2
    Organism Homo sapiens(human)
    Definition Homo sapiens malic enzyme 2 (ME2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001168335.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTTGTCCC GGTTAAGAGT AGTTTCCACC ACTTGTACTT TGGCATGTCG ACATTTGCAC 
    ATAAAAGAAA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG AATGGCATTT
    ACTTTACAAG AACGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA
    CAAGATATTC AAGCCTTACG ATTTCATAGA AACTTGAAGA AAATGACTAG CCCTTTGGAA
    AAATATATCT ACATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA TAGAATACTG
    CAAGATGACA TTGAGAGTTT AATGCCAATT GTATATACAC CGACGGTTGG TCTTGCCTGC
    TCCCAGTATG GACACATCTT TAGAAGACCT AAGGGATTAT TTATTTCGAT CTCAGACAGA
    GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTTGTAGTG
    ACTGATGGAG AGAGAATTCT GGGTCTTGGA GATCTGGGTG TCTATGGAAT GGGAATTCCA
    GTAGGAAAAC TTTGTTTGTA TACAGCTTGT GCAGGAATAC GGCCTGATAG ATGCCTGCCA
    GTGTGTATTG ATGTGGGAAC TGATAATATC GCACTCTTAA AAGACCCATT TTACATGGGC
    TTGTACCAGA AACGAGATCG CACACAACAG TATGATGACC TGATTGATGA GTTTATGAAA
    GCTATTACTG ACAGATATGG CCGGAACACA CTCATTCAGT TCGAAGACTT TGGAAATCAT
    AATGCATTCA GGTTCTTGAG AAAGTACCGA GAAAAATATT GTACTTTCAA TGATGATATT
    CAAGGGACAG CTGCAGTAGC TCTAGCAGGT CTTCTTGCAG CACAAAAAGT TATTAGTAAA
    CCAATCTCCG AACACAAAAT CTTATTCCTT GGAGCAGGAG AGGCTGCTCT TGGAATTGCA
    AATCTTATAG TTATGTCTAT GGTAGAAAAT GGCCTGTCAG AACAAGAGGC ACAAAAGAAA
    ATCTGGATGT TTGACAAGTA TGGTTTATTA GTTAAGGGAC GGAAAGCAAA AATAGATAGT
    TATCAGGAAC CATTTACTCA CTCAGCCCCA GAGAGCATAC CTGATACTTT TGAAGATGCA
    GTGAATATAC TGAAGCCTTC AACTATAATT GGAGTTGCAG GTGCTGGCCG TCTTTTCACT
    CCTGATGTAA TCAGAGCCAT GGCCTCTATC AATGAAAGGC CTGTAATATT TGCATTAAGT
    AATCCTACAG CACAGGCAGA GTGCACGGCT GAAGAAGCAT ATACACTTAC AGAGGGCAGG
    TGTTTGTTTG CCAGTGGCAG TCCATTTGGG CCAGTGAAAC TTACAGATGG GCGAGTCTTT
    ACACCAGGTC AAGGAAACAA TGTTTATATT TTTCCAGGTT ACAGAATACC TATATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.