ME2 cDNA ORF clone, Bos taurus(cattle)

The following ME2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ME2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa39978 NM_001076814.1
Latest version!
Bos taurus malic enzyme 2 (ME2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa46951 XM_010818819.2
Latest version!
Bos taurus malic enzyme 2 (ME2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa229854 XM_024984204.1
Latest version!
Bos taurus malic enzyme 2 (ME2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa229855 XM_024984205.1
Latest version!
Bos taurus malic enzyme 2 (ME2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa229856 XM_024984206.1
Latest version!
Bos taurus malic enzyme 2 (ME2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OBa46951 XM_010818819.1 Bos taurus malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa39978
    Clone ID Related Accession (Same CDS sequence) NM_001076814.1
    Accession Version NM_001076814.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product NAD-dependent malic enzyme, mitochondrial
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC123669.1. On Oct 11, 2006 this sequence version replaced XM_584500.2. ##Evidence-Data-START## Transcript exon combination :: BC123669.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCTGTCCA GGTTTAAAAT AGTCGCCACT CCTTGTACTC TGGCATGCCG CCATTTGCAC 
    ACAAAAGAGA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG GATGGCATTC
    ACATTACAGG AAAGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA
    CAAGATATTC AAGCCTTACG GTTCCATAGA AACTTGAAAA AGTTGAATGG CCCTTTGGAA
    AAATACATCT ATATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA CAGAATACTG
    CAAGATGATA TTGAAACTCT AATGCCAATT GTGTATACAC CAACAGTTGG CCTTGCCTGC
    TCCCAGTATG GACACATCTT TAGAAGACCT AAGGGATTAT TTATTTCAAT CTCAGACAGA
    GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTCGTGGTG
    ACTGATGGAG AGAGAATTCT AGGTCTTGGA GATCTGGGTG TCTATGGAAT GGGAATTCCA
    GTAGGAAAAC TTTGTTTGTA TACAGCTTGT GCGGGAATAC GGCCTGATAG ATGTCTGCCT
    GTGTGTATTG ACGTAGGAAC TGATAATCCG GCCCTCTTAA AGGATCCATT TTATATGGGT
    TTATATCAGA AAAGAGATCG ATCACAGCGT TACGATGACC TAATTGATGA GTTTATGAAG
    GCCATTACTG ACAGATATGG ACAGAACACA CTTATTCAGT TTGAAGACTT TGGAAATCAT
    AATGCATTCA GGTTCTTGAG AAAATACCGA GAAAAATACT GTACCTTCAA CGATGATATT
    CAAGGGACGG CTGCGGTGGC TCTGGCCGGT CTGCTTGCCG CGCAAAAAGT CATTGGGAAA
    CCGATCTCAG ATCATAGAAT CTTATTTCTT GGAGCAGGAG AGGCTGCTCT TGGAATTGCA
    AATCTGATAG TTATGGCTAT GGTTGAAAAT GGCCTCTCAG AAGAAGAGGC ACGAAAGAAA
    ATTTGGATGG TTGACAAGTT TGGTTTATTA TTTAAGGGGC GGAAAGCTAA AATAGAAAGT
    CATCAGGAAG CATTTGCTCA CTCAGCTCCA GAGAGCCTAC CTGATACTTT TGAAGAGGCA
    GTAAATATAC TTAAGCCTTC AGCTATAATT GGAGTTGCAG GAGCTGGCCG ACTTTTCACT
    CCTGGTGTAA TCAAAGCAAT GGCTGAAATC AATGAAAGGC CTATAATATT TGCATTAAGT
    AATCCTACGG CACAGGCTGA GTGCACAGCT GAAGAAGCAT ACACGCTTAC AGAGGGAAGG
    TGTTTGTTTG CCAGTGGCAG TCCATTTGAG CCAGTGAAAC TCAGTGATGG GCAAGTCTTT
    ACACCAGGCC AAGGAAACAA TGTATATATT TTTCCAGGTG TGGCTTTAGC TGTTATTCTC
    TGTAACACCC GGCATATTAG TGACCATGTG TTCCTGGAAG CTGCAAAGGC TCTGACAAGT
    CAGTTGACAG ATAAAGAGCT GGCTCAGGGG AGACTTTACC CACCACTTTC TAATATTCAG
    GAAGTTTCTA TTAACATTGC TATTAAAGTT ACAGAATACC TGTATGCTAA TAAAATGGCT
    TTCCGATACC CAGAACCTGA GGACAAGGCC AAATACATTA GAGAAAGAAT ATGGCGAAGC
    GAGTACGATT CCCTGCTGCC CGATGTGTAT GAGTGGCCAG AGGCTGCAGC GCACCCTCCC
    CCCATAACAG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001070282.1
    CDS268..2022
    Translation

    Target ORF information:

    RefSeq Version NM_001076814.1
    Organism Bos taurus(cattle)
    Definition Bos taurus malic enzyme 2 (ME2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001076814.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCTGTCCA GGTTTAAAAT AGTCGCCACT CCTTGTACTC TGGCATGCCG CCATTTGCAC 
    ACAAAAGAGA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG GATGGCATTC
    ACATTACAGG AAAGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA
    CAAGATATTC AAGCCTTACG GTTCCATAGA AACTTGAAAA AGTTGAATGG CCCTTTGGAA
    AAATACATCT ATATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA CAGAATACTG
    CAAGATGATA TTGAAACTCT AATGCCAATT GTGTATACAC CAACAGTTGG CCTTGCCTGC
    TCCCAGTATG GACACATCTT TAGAAGACCT AAGGGATTAT TTATTTCAAT CTCAGACAGA
    GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTCGTGGTG
    ACTGATGGAG AGAGAATTCT AGGTCTTGGA GATCTGGGTG TCTATGGAAT GGGAATTCCA
    GTAGGAAAAC TTTGTTTGTA TACAGCTTGT GCGGGAATAC GGCCTGATAG ATGTCTGCCT
    GTGTGTATTG ACGTAGGAAC TGATAATCCG GCCCTCTTAA AGGATCCATT TTATATGGGT
    TTATATCAGA AAAGAGATCG ATCACAGCGT TACGATGACC TAATTGATGA GTTTATGAAG
    GCCATTACTG ACAGATATGG ACAGAACACA CTTATTCAGT TTGAAGACTT TGGAAATCAT
    AATGCATTCA GGTTCTTGAG AAAATACCGA GAAAAATACT GTACCTTCAA CGATGATATT
    CAAGGGACGG CTGCGGTGGC TCTGGCCGGT CTGCTTGCCG CGCAAAAAGT CATTGGGAAA
    CCGATCTCAG ATCATAGAAT CTTATTTCTT GGAGCAGGAG AGGCTGCTCT TGGAATTGCA
    AATCTGATAG TTATGGCTAT GGTTGAAAAT GGCCTCTCAG AAGAAGAGGC ACGAAAGAAA
    ATTTGGATGG TTGACAAGTT TGGTTTATTA TTTAAGGGGC GGAAAGCTAA AATAGAAAGT
    CATCAGGAAG CATTTGCTCA CTCAGCTCCA GAGAGCCTAC CTGATACTTT TGAAGAGGCA
    GTAAATATAC TTAAGCCTTC AGCTATAATT GGAGTTGCAG GAGCTGGCCG ACTTTTCACT
    CCTGGTGTAA TCAAAGCAAT GGCTGAAATC AATGAAAGGC CTATAATATT TGCATTAAGT
    AATCCTACGG CACAGGCTGA GTGCACAGCT GAAGAAGCAT ACACGCTTAC AGAGGGAAGG
    TGTTTGTTTG CCAGTGGCAG TCCATTTGAG CCAGTGAAAC TCAGTGATGG GCAAGTCTTT
    ACACCAGGCC AAGGAAACAA TGTATATATT TTTCCAGGTG TGGCTTTAGC TGTTATTCTC
    TGTAACACCC GGCATATTAG TGACCATGTG TTCCTGGAAG CTGCAAAGGC TCTGACAAGT
    CAGTTGACAG ATAAAGAGCT GGCTCAGGGG AGACTTTACC CACCACTTTC TAATATTCAG
    GAAGTTTCTA TTAACATTGC TATTAAAGTT ACAGAATACC TGTATGCTAA TAAAATGGCT
    TTCCGATACC CAGAACCTGA GGACAAGGCC AAATACATTA GAGAAAGAAT ATGGCGAAGC
    GAGTACGATT CCCTGCTGCC CGATGTGTAT GAGTGGCCAG AGGCTGCAGC GCACCCTCCC
    CCCATAACAG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46951
    Clone ID Related Accession (Same CDS sequence) XM_010818819.1 , XM_010818819.2
    Accession Version XM_010818819.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NAD-dependent malic enzyme, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCTGTCCA GGTTTAAAAT AGTCGCCACT CCTTGTACTC TGGCATGCCG CCATTTGCAC 
    ACAAAAGAGA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG GATGGCGTTC
    ACATTACAGG AAAGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA
    CAAGATATTC AAGCCTTACG GTTCCATAGA AACTTGAAAA AGTTGAATGG CCCTTTGGAA
    AAATACATCT ATATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA CAGAATACTG
    CAAGATGATA TTGAAACTCT AATGCCAATT GTGTATACAC CAACAGTTGG CCTTGCCTGC
    TCCCAGTATG GACACATCTT TAGAAGACCT AATTCAAGCA CTTCCAAATA TTTTAAGTGG
    AAAGGATGCT GGGATGAGTG TTGGCTCAAG CCTTTGAGTA AGGGATTATT TATTTCAATC
    TCAGACAGAG GTCATGTTAG ATCAATTGTG GATAACTGGC CAGAAAATCA TGTTAAGGCT
    GTCGTGGTGA CTGATGGAGA GAGAATTCTA GGTCTTGGAG ATCTGGGTGT CTATGGAATG
    GGAATTCCAG TAGGAAAACT TTGTTTGTAT ACAGCTTGTG CGGGAATACG GCCTGATAGA
    TGTCTGCCTG TGTGTATTGA CGTAGGAACT GATAATCCGG CCCTCTTAAA GGATCCATTT
    TATATGGGTT TATATCAGAA AAGAGATCGA TCACAGCGTT ACGATGACCT AATTGATGAG
    TTTATGAAGG CCATTACTGA CAGATATGGA CAGAACACAC TTATTCAGTT TGAAGACTTT
    GGAAATCATA ATGCATTCAG GTTCTTGAGA AAATACCGAG AAAAATACTG TACCTTCAAC
    GATGATATTC AAGGGACGGC TGCGGTGGCT CTGGCCGGTC TGCTTGCCGC GCAAAAAGTC
    ATTGGGAAAC CGATCTCAGA TCATAGAATC TTATTTCTTG GAGCAGGAGA GGCTGCTCTT
    GGAATTGCAA ATCTGATAGT TATGGCTATG GTTGAAAATG GCCTCTCAGA AGAAGAGGCA
    CGAAAGAAAA TTTGGATGGT TGACAAGTTT GGTTTATTAT TTAAGGGGCG GAAAGCTAAA
    ATAGAAAGTC ATCAGGAAGC ATTTGCTCAC TCAGCTCCAG AGAGCCTACC TGATACTTTT
    GAAGAGGCAG TAAATATACT TAAGCCTTCA GCTATAATTG GAGTTGCAGG AGCTGGCCGA
    CTTTTCACTC CTGGTGTAAT CAAAGCAATG GCTGAAATCA ATGAAAGGCC TATAATATTT
    GCATTAAGTA ATCCTACGGC ACAGGCTGAG TGCACAGCTG AAGAAGCATA CACGCTTACA
    GAGGGAAGGT GTTTGTTTGC CAGTGGCAGT CCGTTTGAGC CAGTGAAACT CAGTGATGGG
    CAAGTCTTTA CACCAGGCCA AGGAAACAAT GTATATATTT TTCCAGGTGT GGCTTTAGCT
    GTTATTCTCT GTAACACCCG GCATATTAGT GACCATGTGT TCCTGGAAGC TGCAAAGGCT
    CTGACAAGTC AGTTGACAGA TAAAGAGCTG GCTCAGGGGA GACTTTACCC ACCACTTTCT
    AATATTCAGG AAGTTTCTAT TAACATTGCT ATTAAAGTTA CAGAATACCT GTATGCTAAT
    AAAATGGCTT TCCGATACCC AGAACCTGAG GACAAGGCCA AATACATTAG AGAAAGAATA
    TGGCGAAGCG AGTACGATTC CCTGCTGCCC GATGTGTATG AGTGGCCAGA GGCTGCAGCG
    CACCCTCCCC CCATAACAGA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817121.1
    CDS112..1935
    Translation

    Target ORF information:

    RefSeq Version XM_010818819.1
    Organism Bos taurus(cattle)
    Definition Bos taurus malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818819.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCTGTCCA GGTTTAAAAT AGTCGCCACT CCTTGTACTC TGGCATGCCG CCATTTGCAC 
    ACAAAAGAGA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG GATGGCGTTC
    ACATTACAGG AAAGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA
    CAAGATATTC AAGCCTTACG GTTCCATAGA AACTTGAAAA AGTTGAATGG CCCTTTGGAA
    AAATACATCT ATATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA CAGAATACTG
    CAAGATGATA TTGAAACTCT AATGCCAATT GTGTATACAC CAACAGTTGG CCTTGCCTGC
    TCCCAGTATG GACACATCTT TAGAAGACCT AATTCAAGCA CTTCCAAATA TTTTAAGTGG
    AAAGGATGCT GGGATGAGTG TTGGCTCAAG CCTTTGAGTA AGGGATTATT TATTTCAATC
    TCAGACAGAG GTCATGTTAG ATCAATTGTG GATAACTGGC CAGAAAATCA TGTTAAGGCT
    GTCGTGGTGA CTGATGGAGA GAGAATTCTA GGTCTTGGAG ATCTGGGTGT CTATGGAATG
    GGAATTCCAG TAGGAAAACT TTGTTTGTAT ACAGCTTGTG CGGGAATACG GCCTGATAGA
    TGTCTGCCTG TGTGTATTGA CGTAGGAACT GATAATCCGG CCCTCTTAAA GGATCCATTT
    TATATGGGTT TATATCAGAA AAGAGATCGA TCACAGCGTT ACGATGACCT AATTGATGAG
    TTTATGAAGG CCATTACTGA CAGATATGGA CAGAACACAC TTATTCAGTT TGAAGACTTT
    GGAAATCATA ATGCATTCAG GTTCTTGAGA AAATACCGAG AAAAATACTG TACCTTCAAC
    GATGATATTC AAGGGACGGC TGCGGTGGCT CTGGCCGGTC TGCTTGCCGC GCAAAAAGTC
    ATTGGGAAAC CGATCTCAGA TCATAGAATC TTATTTCTTG GAGCAGGAGA GGCTGCTCTT
    GGAATTGCAA ATCTGATAGT TATGGCTATG GTTGAAAATG GCCTCTCAGA AGAAGAGGCA
    CGAAAGAAAA TTTGGATGGT TGACAAGTTT GGTTTATTAT TTAAGGGGCG GAAAGCTAAA
    ATAGAAAGTC ATCAGGAAGC ATTTGCTCAC TCAGCTCCAG AGAGCCTACC TGATACTTTT
    GAAGAGGCAG TAAATATACT TAAGCCTTCA GCTATAATTG GAGTTGCAGG AGCTGGCCGA
    CTTTTCACTC CTGGTGTAAT CAAAGCAATG GCTGAAATCA ATGAAAGGCC TATAATATTT
    GCATTAAGTA ATCCTACGGC ACAGGCTGAG TGCACAGCTG AAGAAGCATA CACGCTTACA
    GAGGGAAGGT GTTTGTTTGC CAGTGGCAGT CCGTTTGAGC CAGTGAAACT CAGTGATGGG
    CAAGTCTTTA CACCAGGCCA AGGAAACAAT GTATATATTT TTCCAGGTGT GGCTTTAGCT
    GTTATTCTCT GTAACACCCG GCATATTAGT GACCATGTGT TCCTGGAAGC TGCAAAGGCT
    CTGACAAGTC AGTTGACAGA TAAAGAGCTG GCTCAGGGGA GACTTTACCC ACCACTTTCT
    AATATTCAGG AAGTTTCTAT TAACATTGCT ATTAAAGTTA CAGAATACCT GTATGCTAAT
    AAAATGGCTT TCCGATACCC AGAACCTGAG GACAAGGCCA AATACATTAG AGAAAGAATA
    TGGCGAAGCG AGTACGATTC CCTGCTGCCC GATGTGTATG AGTGGCCAGA GGCTGCAGCG
    CACCCTCCCC CCATAACAGA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46951
    Clone ID Related Accession (Same CDS sequence) XM_010818819.1 , XM_010818819.2
    Accession Version XM_010818819.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NAD-dependent malic enzyme, mitochondrial isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818819.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCTGTCCA GGTTTAAAAT AGTCGCCACT CCTTGTACTC TGGCATGCCG CCATTTGCAC 
    ACAAAAGAGA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG GATGGCGTTC
    ACATTACAGG AAAGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA
    CAAGATATTC AAGCCTTACG GTTCCATAGA AACTTGAAAA AGTTGAATGG CCCTTTGGAA
    AAATACATCT ATATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA CAGAATACTG
    CAAGATGATA TTGAAACTCT AATGCCAATT GTGTATACAC CAACAGTTGG CCTTGCCTGC
    TCCCAGTATG GACACATCTT TAGAAGACCT AATTCAAGCA CTTCCAAATA TTTTAAGTGG
    AAAGGATGCT GGGATGAGTG TTGGCTCAAG CCTTTGAGTA AGGGATTATT TATTTCAATC
    TCAGACAGAG GTCATGTTAG ATCAATTGTG GATAACTGGC CAGAAAATCA TGTTAAGGCT
    GTCGTGGTGA CTGATGGAGA GAGAATTCTA GGTCTTGGAG ATCTGGGTGT CTATGGAATG
    GGAATTCCAG TAGGAAAACT TTGTTTGTAT ACAGCTTGTG CGGGAATACG GCCTGATAGA
    TGTCTGCCTG TGTGTATTGA CGTAGGAACT GATAATCCGG CCCTCTTAAA GGATCCATTT
    TATATGGGTT TATATCAGAA AAGAGATCGA TCACAGCGTT ACGATGACCT AATTGATGAG
    TTTATGAAGG CCATTACTGA CAGATATGGA CAGAACACAC TTATTCAGTT TGAAGACTTT
    GGAAATCATA ATGCATTCAG GTTCTTGAGA AAATACCGAG AAAAATACTG TACCTTCAAC
    GATGATATTC AAGGGACGGC TGCGGTGGCT CTGGCCGGTC TGCTTGCCGC GCAAAAAGTC
    ATTGGGAAAC CGATCTCAGA TCATAGAATC TTATTTCTTG GAGCAGGAGA GGCTGCTCTT
    GGAATTGCAA ATCTGATAGT TATGGCTATG GTTGAAAATG GCCTCTCAGA AGAAGAGGCA
    CGAAAGAAAA TTTGGATGGT TGACAAGTTT GGTTTATTAT TTAAGGGGCG GAAAGCTAAA
    ATAGAAAGTC ATCAGGAAGC ATTTGCTCAC TCAGCTCCAG AGAGCCTACC TGATACTTTT
    GAAGAGGCAG TAAATATACT TAAGCCTTCA GCTATAATTG GAGTTGCAGG AGCTGGCCGA
    CTTTTCACTC CTGGTGTAAT CAAAGCAATG GCTGAAATCA ATGAAAGGCC TATAATATTT
    GCATTAAGTA ATCCTACGGC ACAGGCTGAG TGCACAGCTG AAGAAGCATA CACGCTTACA
    GAGGGAAGGT GTTTGTTTGC CAGTGGCAGT CCGTTTGAGC CAGTGAAACT CAGTGATGGG
    CAAGTCTTTA CACCAGGCCA AGGAAACAAT GTATATATTT TTCCAGGTGT GGCTTTAGCT
    GTTATTCTCT GTAACACCCG GCATATTAGT GACCATGTGT TCCTGGAAGC TGCAAAGGCT
    CTGACAAGTC AGTTGACAGA TAAAGAGCTG GCTCAGGGGA GACTTTACCC ACCACTTTCT
    AATATTCAGG AAGTTTCTAT TAACATTGCT ATTAAAGTTA CAGAATACCT GTATGCTAAT
    AAAATGGCTT TCCGATACCC AGAACCTGAG GACAAGGCCA AATACATTAG AGAAAGAATA
    TGGCGAAGCG AGTACGATTC CCTGCTGCCC GATGTGTATG AGTGGCCAGA GGCTGCAGCG
    CACCCTCCCC CCATAACAGA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817121.1
    CDS530..2353
    Translation

    Target ORF information:

    RefSeq Version XM_010818819.2
    Organism Bos taurus(cattle)
    Definition Bos taurus malic enzyme 2 (ME2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818819.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCTGTCCA GGTTTAAAAT AGTCGCCACT CCTTGTACTC TGGCATGCCG CCATTTGCAC 
    ACAAAAGAGA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG GATGGCGTTC
    ACATTACAGG AAAGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA
    CAAGATATTC AAGCCTTACG GTTCCATAGA AACTTGAAAA AGTTGAATGG CCCTTTGGAA
    AAATACATCT ATATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA CAGAATACTG
    CAAGATGATA TTGAAACTCT AATGCCAATT GTGTATACAC CAACAGTTGG CCTTGCCTGC
    TCCCAGTATG GACACATCTT TAGAAGACCT AATTCAAGCA CTTCCAAATA TTTTAAGTGG
    AAAGGATGCT GGGATGAGTG TTGGCTCAAG CCTTTGAGTA AGGGATTATT TATTTCAATC
    TCAGACAGAG GTCATGTTAG ATCAATTGTG GATAACTGGC CAGAAAATCA TGTTAAGGCT
    GTCGTGGTGA CTGATGGAGA GAGAATTCTA GGTCTTGGAG ATCTGGGTGT CTATGGAATG
    GGAATTCCAG TAGGAAAACT TTGTTTGTAT ACAGCTTGTG CGGGAATACG GCCTGATAGA
    TGTCTGCCTG TGTGTATTGA CGTAGGAACT GATAATCCGG CCCTCTTAAA GGATCCATTT
    TATATGGGTT TATATCAGAA AAGAGATCGA TCACAGCGTT ACGATGACCT AATTGATGAG
    TTTATGAAGG CCATTACTGA CAGATATGGA CAGAACACAC TTATTCAGTT TGAAGACTTT
    GGAAATCATA ATGCATTCAG GTTCTTGAGA AAATACCGAG AAAAATACTG TACCTTCAAC
    GATGATATTC AAGGGACGGC TGCGGTGGCT CTGGCCGGTC TGCTTGCCGC GCAAAAAGTC
    ATTGGGAAAC CGATCTCAGA TCATAGAATC TTATTTCTTG GAGCAGGAGA GGCTGCTCTT
    GGAATTGCAA ATCTGATAGT TATGGCTATG GTTGAAAATG GCCTCTCAGA AGAAGAGGCA
    CGAAAGAAAA TTTGGATGGT TGACAAGTTT GGTTTATTAT TTAAGGGGCG GAAAGCTAAA
    ATAGAAAGTC ATCAGGAAGC ATTTGCTCAC TCAGCTCCAG AGAGCCTACC TGATACTTTT
    GAAGAGGCAG TAAATATACT TAAGCCTTCA GCTATAATTG GAGTTGCAGG AGCTGGCCGA
    CTTTTCACTC CTGGTGTAAT CAAAGCAATG GCTGAAATCA ATGAAAGGCC TATAATATTT
    GCATTAAGTA ATCCTACGGC ACAGGCTGAG TGCACAGCTG AAGAAGCATA CACGCTTACA
    GAGGGAAGGT GTTTGTTTGC CAGTGGCAGT CCGTTTGAGC CAGTGAAACT CAGTGATGGG
    CAAGTCTTTA CACCAGGCCA AGGAAACAAT GTATATATTT TTCCAGGTGT GGCTTTAGCT
    GTTATTCTCT GTAACACCCG GCATATTAGT GACCATGTGT TCCTGGAAGC TGCAAAGGCT
    CTGACAAGTC AGTTGACAGA TAAAGAGCTG GCTCAGGGGA GACTTTACCC ACCACTTTCT
    AATATTCAGG AAGTTTCTAT TAACATTGCT ATTAAAGTTA CAGAATACCT GTATGCTAAT
    AAAATGGCTT TCCGATACCC AGAACCTGAG GACAAGGCCA AATACATTAG AGAAAGAATA
    TGGCGAAGCG AGTACGATTC CCTGCTGCCC GATGTGTATG AGTGGCCAGA GGCTGCAGCG
    CACCCTCCCC CCATAACAGA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229854
    Clone ID Related Accession (Same CDS sequence) XM_024984204.1
    Accession Version XM_024984204.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NAD-dependent malic enzyme, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCCGCTGC TGCTACCGCT GCTGCTAAGT CGCTTCAGTT GTGTCCGACT CTGCCTACTC 
    TGCAGCCTAC CAGGCTCCTC CGCCCATGGG ATTTTCCAGG TGAGAAAAAA GATGCTGTCC
    AGGTTTAAAA TAGTCGCCAC TCCTTGTACT CTGGCATGCC GCCATTTGCA CACAAAAGAG
    AAAGGCAAGC CACTTATGCT GAACCCAAGA ACAAACAAGG GGATGGCGTT CACATTACAG
    GAAAGACAAA TGCTTGGTCT TCAAGGACTT CTACCTCCCA AAATAGAGAC ACAAGATATT
    CAAGCCTTAC GGTTCCATAG AAACTTGAAA AAGTTGAATG GCCCTTTGGA AAAATACATC
    TATATAATGG GAATACAAGA AAGAAATGAG AAATTGTTTT ACAGAATACT GCAAGATGAT
    ATTGAAACTC TAATGCCAAT TGTGTATACA CCAACAGTTG GCCTTGCCTG CTCCCAGTAT
    GGACACATCT TTAGAAGACC TAATTCAAGC ACTTCCAAAT ATTTTAAGTG GAAAGGATGC
    TGGGATGAGT GTTGGCTCAA GCCTTTGAGT AAGGGATTAT TTATTTCAAT CTCAGACAGA
    GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTCGTGGTG
    ACTGATGGAG AGAGAATTCT AGGTCTTGGA GATCTGGGTG TCTATGGAAT GGGAATTCCA
    GTAGGAAAAC TTTGTTTGTA TACAGCTTGT GCGGGAATAC GGCCTGATAG ATGTCTGCCT
    GTGTGTATTG ACGTAGGAAC TGATAATCCG GCCCTCTTAA AGGATCCATT TTATATGGGT
    TTATATCAGA AAAGAGATCG ATCACAGCGT TACGATGACC TAATTGATGA GTTTATGAAG
    GCCATTACTG ACAGATATGG ACAGAACACA CTTATTCAGT TTGAAGACTT TGGAAATCAT
    AATGCATTCA GGTTCTTGAG AAAATACCGA GAAAAATACT GTACCTTCAA CGATGATATT
    CAAGGGACGG CTGCGGTGGC TCTGGCCGGT CTGCTTGCCG CGCAAAAAGT CATTGGGAAA
    CCGATCTCAG ATCATAGAAT CTTATTTCTT GGAGCAGGAG AGGCTGCTCT TGGAATTGCA
    AATCTGATAG TTATGGCTAT GGTTGAAAAT GGCCTCTCAG AAGAAGAGGC ACGAAAGAAA
    ATTTGGATGG TTGACAAGTT TGGTTTATTA TTTAAGGGGC GGAAAGCTAA AATAGAAAGT
    CATCAGGAAG CATTTGCTCA CTCAGCTCCA GAGAGCCTAC CTGATACTTT TGAAGAGGCA
    GTAAATATAC TTAAGCCTTC AGCTATAATT GGAGTTGCAG GAGCTGGCCG ACTTTTCACT
    CCTGGTGTAA TCAAAGCAAT GGCTGAAATC AATGAAAGGC CTATAATATT TGCATTAAGT
    AATCCTACGG CACAGGCTGA GTGCACAGCT GAAGAAGCAT ACACGCTTAC AGAGGGAAGG
    TGTTTGTTTG CCAGTGGCAG TCCGTTTGAG CCAGTGAAAC TCAGTGATGG GCAAGTCTTT
    ACACCAGGCC AAGGAAACAA TGTATATATT TTTCCAGGTG TGGCTTTAGC TGTTATTCTC
    TGTAACACCC GGCATATTAG TGACCATGTG TTCCTGGAAG CTGCAAAGGC TCTGACAAGT
    CAGTTGACAG ATAAAGAGCT GGCTCAGGGG AGACTTTACC CACCACTTTC TAATATTCAG
    GAAGTTTCTA TTAACATTGC TATTAAAGTT ACAGAATACC TGTATGCTAA TAAAATGGCT
    TTCCGATACC CAGAACCTGA GGACAAGGCC AAATACATTA GAGAAAGAAT ATGGCGAAGC
    GAGTACGATT CCCTGCTGCC CGATGTGTAT GAGTGGCCAG AGGCTGCAGC GCACCCTCCC
    CCCATAACAG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839972.1
    CDS188..2122
    Translation

    Target ORF information:

    RefSeq Version XM_024984204.1
    Organism Bos taurus(cattle)
    Definition Bos taurus malic enzyme 2 (ME2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984204.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCCGCTGC TGCTACCGCT GCTGCTAAGT CGCTTCAGTT GTGTCCGACT CTGCCTACTC 
    TGCAGCCTAC CAGGCTCCTC CGCCCATGGG ATTTTCCAGG TGAGAAAAAA GATGCTGTCC
    AGGTTTAAAA TAGTCGCCAC TCCTTGTACT CTGGCATGCC GCCATTTGCA CACAAAAGAG
    AAAGGCAAGC CACTTATGCT GAACCCAAGA ACAAACAAGG GGATGGCGTT CACATTACAG
    GAAAGACAAA TGCTTGGTCT TCAAGGACTT CTACCTCCCA AAATAGAGAC ACAAGATATT
    CAAGCCTTAC GGTTCCATAG AAACTTGAAA AAGTTGAATG GCCCTTTGGA AAAATACATC
    TATATAATGG GAATACAAGA AAGAAATGAG AAATTGTTTT ACAGAATACT GCAAGATGAT
    ATTGAAACTC TAATGCCAAT TGTGTATACA CCAACAGTTG GCCTTGCCTG CTCCCAGTAT
    GGACACATCT TTAGAAGACC TAATTCAAGC ACTTCCAAAT ATTTTAAGTG GAAAGGATGC
    TGGGATGAGT GTTGGCTCAA GCCTTTGAGT AAGGGATTAT TTATTTCAAT CTCAGACAGA
    GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTCGTGGTG
    ACTGATGGAG AGAGAATTCT AGGTCTTGGA GATCTGGGTG TCTATGGAAT GGGAATTCCA
    GTAGGAAAAC TTTGTTTGTA TACAGCTTGT GCGGGAATAC GGCCTGATAG ATGTCTGCCT
    GTGTGTATTG ACGTAGGAAC TGATAATCCG GCCCTCTTAA AGGATCCATT TTATATGGGT
    TTATATCAGA AAAGAGATCG ATCACAGCGT TACGATGACC TAATTGATGA GTTTATGAAG
    GCCATTACTG ACAGATATGG ACAGAACACA CTTATTCAGT TTGAAGACTT TGGAAATCAT
    AATGCATTCA GGTTCTTGAG AAAATACCGA GAAAAATACT GTACCTTCAA CGATGATATT
    CAAGGGACGG CTGCGGTGGC TCTGGCCGGT CTGCTTGCCG CGCAAAAAGT CATTGGGAAA
    CCGATCTCAG ATCATAGAAT CTTATTTCTT GGAGCAGGAG AGGCTGCTCT TGGAATTGCA
    AATCTGATAG TTATGGCTAT GGTTGAAAAT GGCCTCTCAG AAGAAGAGGC ACGAAAGAAA
    ATTTGGATGG TTGACAAGTT TGGTTTATTA TTTAAGGGGC GGAAAGCTAA AATAGAAAGT
    CATCAGGAAG CATTTGCTCA CTCAGCTCCA GAGAGCCTAC CTGATACTTT TGAAGAGGCA
    GTAAATATAC TTAAGCCTTC AGCTATAATT GGAGTTGCAG GAGCTGGCCG ACTTTTCACT
    CCTGGTGTAA TCAAAGCAAT GGCTGAAATC AATGAAAGGC CTATAATATT TGCATTAAGT
    AATCCTACGG CACAGGCTGA GTGCACAGCT GAAGAAGCAT ACACGCTTAC AGAGGGAAGG
    TGTTTGTTTG CCAGTGGCAG TCCGTTTGAG CCAGTGAAAC TCAGTGATGG GCAAGTCTTT
    ACACCAGGCC AAGGAAACAA TGTATATATT TTTCCAGGTG TGGCTTTAGC TGTTATTCTC
    TGTAACACCC GGCATATTAG TGACCATGTG TTCCTGGAAG CTGCAAAGGC TCTGACAAGT
    CAGTTGACAG ATAAAGAGCT GGCTCAGGGG AGACTTTACC CACCACTTTC TAATATTCAG
    GAAGTTTCTA TTAACATTGC TATTAAAGTT ACAGAATACC TGTATGCTAA TAAAATGGCT
    TTCCGATACC CAGAACCTGA GGACAAGGCC AAATACATTA GAGAAAGAAT ATGGCGAAGC
    GAGTACGATT CCCTGCTGCC CGATGTGTAT GAGTGGCCAG AGGCTGCAGC GCACCCTCCC
    CCCATAACAG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229855
    Clone ID Related Accession (Same CDS sequence) XM_024984205.1
    Accession Version XM_024984205.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NAD-dependent malic enzyme, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGACACACC AGAGGAAGAA ACCACAGGCA TACCTCAACA TCCAGGAAAC TCGAGTAGTG 
    AGAAAAAAGA TGCTGTCCAG GTTTAAAATA GTCGCCACTC CTTGTACTCT GGCATGCCGC
    CATTTGCACA CAAAAGAGAA AGGCAAGCCA CTTATGCTGA ACCCAAGAAC AAACAAGGGG
    ATGGCGTTCA CATTACAGGA AAGACAAATG CTTGGTCTTC AAGGACTTCT ACCTCCCAAA
    ATAGAGACAC AAGATATTCA AGCCTTACGG TTCCATAGAA ACTTGAAAAA GTTGAATGGC
    CCTTTGGAAA AATACATCTA TATAATGGGA ATACAAGAAA GAAATGAGAA ATTGTTTTAC
    AGAATACTGC AAGATGATAT TGAAACTCTA ATGCCAATTG TGTATACACC AACAGTTGGC
    CTTGCCTGCT CCCAGTATGG ACACATCTTT AGAAGACCTA ATTCAAGCAC TTCCAAATAT
    TTTAAGTGGA AAGGATGCTG GGATGAGTGT TGGCTCAAGC CTTTGAGTAA GGGATTATTT
    ATTTCAATCT CAGACAGAGG TCATGTTAGA TCAATTGTGG ATAACTGGCC AGAAAATCAT
    GTTAAGGCTG TCGTGGTGAC TGATGGAGAG AGAATTCTAG GTCTTGGAGA TCTGGGTGTC
    TATGGAATGG GAATTCCAGT AGGAAAACTT TGTTTGTATA CAGCTTGTGC GGGAATACGG
    CCTGATAGAT GTCTGCCTGT GTGTATTGAC GTAGGAACTG ATAATCCGGC CCTCTTAAAG
    GATCCATTTT ATATGGGTTT ATATCAGAAA AGAGATCGAT CACAGCGTTA CGATGACCTA
    ATTGATGAGT TTATGAAGGC CATTACTGAC AGATATGGAC AGAACACACT TATTCAGTTT
    GAAGACTTTG GAAATCATAA TGCATTCAGG TTCTTGAGAA AATACCGAGA AAAATACTGT
    ACCTTCAACG ATGATATTCA AGGGACGGCT GCGGTGGCTC TGGCCGGTCT GCTTGCCGCG
    CAAAAAGTCA TTGGGAAACC GATCTCAGAT CATAGAATCT TATTTCTTGG AGCAGGAGAG
    GCTGCTCTTG GAATTGCAAA TCTGATAGTT ATGGCTATGG TTGAAAATGG CCTCTCAGAA
    GAAGAGGCAC GAAAGAAAAT TTGGATGGTT GACAAGTTTG GTTTATTATT TAAGGGGCGG
    AAAGCTAAAA TAGAAAGTCA TCAGGAAGCA TTTGCTCACT CAGCTCCAGA GAGCCTACCT
    GATACTTTTG AAGAGGCAGT AAATATACTT AAGCCTTCAG CTATAATTGG AGTTGCAGGA
    GCTGGCCGAC TTTTCACTCC TGGTGTAATC AAAGCAATGG CTGAAATCAA TGAAAGGCCT
    ATAATATTTG CATTAAGTAA TCCTACGGCA CAGGCTGAGT GCACAGCTGA AGAAGCATAC
    ACGCTTACAG AGGGAAGGTG TTTGTTTGCC AGTGGCAGTC CGTTTGAGCC AGTGAAACTC
    AGTGATGGGC AAGTCTTTAC ACCAGGCCAA GGAAACAATG TATATATTTT TCCAGGTGTG
    GCTTTAGCTG TTATTCTCTG TAACACCCGG CATATTAGTG ACCATGTGTT CCTGGAAGCT
    GCAAAGGCTC TGACAAGTCA GTTGACAGAT AAAGAGCTGG CTCAGGGGAG ACTTTACCCA
    CCACTTTCTA ATATTCAGGA AGTTTCTATT AACATTGCTA TTAAAGTTAC AGAATACCTG
    TATGCTAATA AAATGGCTTT CCGATACCCA GAACCTGAGG ACAAGGCCAA ATACATTAGA
    GAAAGAATAT GGCGAAGCGA GTACGATTCC CTGCTGCCCG ATGTGTATGA GTGGCCAGAG
    GCTGCAGCGC ACCCTCCCCC CATAACAGAA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839973.1
    CDS63..1955
    Translation

    Target ORF information:

    RefSeq Version XM_024984205.1
    Organism Bos taurus(cattle)
    Definition Bos taurus malic enzyme 2 (ME2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984205.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGACACACC AGAGGAAGAA ACCACAGGCA TACCTCAACA TCCAGGAAAC TCGAGTAGTG 
    AGAAAAAAGA TGCTGTCCAG GTTTAAAATA GTCGCCACTC CTTGTACTCT GGCATGCCGC
    CATTTGCACA CAAAAGAGAA AGGCAAGCCA CTTATGCTGA ACCCAAGAAC AAACAAGGGG
    ATGGCGTTCA CATTACAGGA AAGACAAATG CTTGGTCTTC AAGGACTTCT ACCTCCCAAA
    ATAGAGACAC AAGATATTCA AGCCTTACGG TTCCATAGAA ACTTGAAAAA GTTGAATGGC
    CCTTTGGAAA AATACATCTA TATAATGGGA ATACAAGAAA GAAATGAGAA ATTGTTTTAC
    AGAATACTGC AAGATGATAT TGAAACTCTA ATGCCAATTG TGTATACACC AACAGTTGGC
    CTTGCCTGCT CCCAGTATGG ACACATCTTT AGAAGACCTA ATTCAAGCAC TTCCAAATAT
    TTTAAGTGGA AAGGATGCTG GGATGAGTGT TGGCTCAAGC CTTTGAGTAA GGGATTATTT
    ATTTCAATCT CAGACAGAGG TCATGTTAGA TCAATTGTGG ATAACTGGCC AGAAAATCAT
    GTTAAGGCTG TCGTGGTGAC TGATGGAGAG AGAATTCTAG GTCTTGGAGA TCTGGGTGTC
    TATGGAATGG GAATTCCAGT AGGAAAACTT TGTTTGTATA CAGCTTGTGC GGGAATACGG
    CCTGATAGAT GTCTGCCTGT GTGTATTGAC GTAGGAACTG ATAATCCGGC CCTCTTAAAG
    GATCCATTTT ATATGGGTTT ATATCAGAAA AGAGATCGAT CACAGCGTTA CGATGACCTA
    ATTGATGAGT TTATGAAGGC CATTACTGAC AGATATGGAC AGAACACACT TATTCAGTTT
    GAAGACTTTG GAAATCATAA TGCATTCAGG TTCTTGAGAA AATACCGAGA AAAATACTGT
    ACCTTCAACG ATGATATTCA AGGGACGGCT GCGGTGGCTC TGGCCGGTCT GCTTGCCGCG
    CAAAAAGTCA TTGGGAAACC GATCTCAGAT CATAGAATCT TATTTCTTGG AGCAGGAGAG
    GCTGCTCTTG GAATTGCAAA TCTGATAGTT ATGGCTATGG TTGAAAATGG CCTCTCAGAA
    GAAGAGGCAC GAAAGAAAAT TTGGATGGTT GACAAGTTTG GTTTATTATT TAAGGGGCGG
    AAAGCTAAAA TAGAAAGTCA TCAGGAAGCA TTTGCTCACT CAGCTCCAGA GAGCCTACCT
    GATACTTTTG AAGAGGCAGT AAATATACTT AAGCCTTCAG CTATAATTGG AGTTGCAGGA
    GCTGGCCGAC TTTTCACTCC TGGTGTAATC AAAGCAATGG CTGAAATCAA TGAAAGGCCT
    ATAATATTTG CATTAAGTAA TCCTACGGCA CAGGCTGAGT GCACAGCTGA AGAAGCATAC
    ACGCTTACAG AGGGAAGGTG TTTGTTTGCC AGTGGCAGTC CGTTTGAGCC AGTGAAACTC
    AGTGATGGGC AAGTCTTTAC ACCAGGCCAA GGAAACAATG TATATATTTT TCCAGGTGTG
    GCTTTAGCTG TTATTCTCTG TAACACCCGG CATATTAGTG ACCATGTGTT CCTGGAAGCT
    GCAAAGGCTC TGACAAGTCA GTTGACAGAT AAAGAGCTGG CTCAGGGGAG ACTTTACCCA
    CCACTTTCTA ATATTCAGGA AGTTTCTATT AACATTGCTA TTAAAGTTAC AGAATACCTG
    TATGCTAATA AAATGGCTTT CCGATACCCA GAACCTGAGG ACAAGGCCAA ATACATTAGA
    GAAAGAATAT GGCGAAGCGA GTACGATTCC CTGCTGCCCG ATGTGTATGA GTGGCCAGAG
    GCTGCAGCGC ACCCTCCCCC CATAACAGAA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229856
    Clone ID Related Accession (Same CDS sequence) XM_024984206.1
    Accession Version XM_024984206.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NAD-dependent malic enzyme, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCGCTGC TGCTACCGCT GCTGCTAAGT CGCTTCAGTT GTGTCCGACT CTGCCTACTC 
    TGCAGCCTAC CAGGCTCCTC CGCCCATGGG ATTTTCCAGG TGAGAAAAAA GATGCTGTCC
    AGGTTTAAAA TAGTCGCCAC TCCTTGTACT CTGGCATGCC GCCATTTGCA CACAAAAGAG
    AAAGGCAAGC CACTTATGCT GAACCCAAGA ACAAACAAGG GGATGGCGTT CACATTACAG
    GAAAGACAAA TGCTTGGTCT TCAAGGACTT CTACCTCCCA AAATAGAGAC ACAAGATATT
    CAAGCCTTAC GGTTCCATAG AAACTTGAAA AAGTTGAATG GCCCTTTGGA AAAATACATC
    TATATAATGG GAATACAAGA AAGAAATGAG AAATTGTTTT ACAGAATACT GCAAGATGAT
    ATTGAAACTC TAATGCCAAT TGTGTATACA CCAACAGTTG GCCTTGCCTG CTCCCAGTAT
    GGACACATCT TTAGAAGACC TAAGGGATTA TTTATTTCAA TCTCAGACAG AGGTCATGTT
    AGATCAATTG TGGATAACTG GCCAGAAAAT CATGTTAAGG CTGTCGTGGT GACTGATGGA
    GAGAGAATTC TAGGTCTTGG AGATCTGGGT GTCTATGGAA TGGGAATTCC AGTAGGAAAA
    CTTTGTTTGT ATACAGCTTG TGCGGGAATA CGGCCTGATA GATGTCTGCC TGTGTGTATT
    GACGTAGGAA CTGATAATCC GGCCCTCTTA AAGGATCCAT TTTATATGGG TTTATATCAG
    AAAAGAGATC GATCACAGCG TTACGATGAC CTAATTGATG AGTTTATGAA GGCCATTACT
    GACAGATATG GACAGAACAC ACTTATTCAG TTTGAAGACT TTGGAAATCA TAATGCATTC
    AGGTTCTTGA GAAAATACCG AGAAAAATAC TGTACCTTCA ACGATGATAT TCAAGGGACG
    GCTGCGGTGG CTCTGGCCGG TCTGCTTGCC GCGCAAAAAG TCATTGGGAA ACCGATCTCA
    GATCATAGAA TCTTATTTCT TGGAGCAGGA GAGGCTGCTC TTGGAATTGC AAATCTGATA
    GTTATGGCTA TGGTTGAAAA TGGCCTCTCA GAAGAAGAGG CACGAAAGAA AATTTGGATG
    GTTGACAAGT TTGGTTTATT ATTTAAGGGG CGGAAAGCTA AAATAGAAAG TCATCAGGAA
    GCATTTGCTC ACTCAGCTCC AGAGAGCCTA CCTGATACTT TTGAAGAGGC AGTAAATATA
    CTTAAGCCTT CAGCTATAAT TGGAGTTGCA GGAGCTGGCC GACTTTTCAC TCCTGGTGTA
    ATCAAAGCAA TGGCTGAAAT CAATGAAAGG CCTATAATAT TTGCATTAAG TAATCCTACG
    GCACAGGCTG AGTGCACAGC TGAAGAAGCA TACACGCTTA CAGAGGGAAG GTGTTTGTTT
    GCCAGTGGCA GTCCGTTTGA GCCAGTGAAA CTCAGTGATG GGCAAGTCTT TACACCAGGC
    CAAGGAAACA ATGTATATAT TTTTCCAGGT GTGGCTTTAG CTGTTATTCT CTGTAACACC
    CGGCATATTA GTGACCATGT GTTCCTGGAA GCTGCAAAGG CTCTGACAAG TCAGTTGACA
    GATAAAGAGC TGGCTCAGGG GAGACTTTAC CCACCACTTT CTAATATTCA GGAAGTTTCT
    ATTAACATTG CTATTAAAGT TACAGAATAC CTGTATGCTA ATAAAATGGC TTTCCGATAC
    CCAGAACCTG AGGACAAGGC CAAATACATT AGAGAAAGAA TATGGCGAAG CGAGTACGAT
    TCCCTGCTGC CCGATGTGTA TGAGTGGCCA GAGGCTGCAG CGCACCCTCC CCCCATAACA
    GAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839974.1
    CDS293..2158
    Translation

    Target ORF information:

    RefSeq Version XM_024984206.1
    Organism Bos taurus(cattle)
    Definition Bos taurus malic enzyme 2 (ME2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984206.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCGCTGC TGCTACCGCT GCTGCTAAGT CGCTTCAGTT GTGTCCGACT CTGCCTACTC 
    TGCAGCCTAC CAGGCTCCTC CGCCCATGGG ATTTTCCAGG TGAGAAAAAA GATGCTGTCC
    AGGTTTAAAA TAGTCGCCAC TCCTTGTACT CTGGCATGCC GCCATTTGCA CACAAAAGAG
    AAAGGCAAGC CACTTATGCT GAACCCAAGA ACAAACAAGG GGATGGCGTT CACATTACAG
    GAAAGACAAA TGCTTGGTCT TCAAGGACTT CTACCTCCCA AAATAGAGAC ACAAGATATT
    CAAGCCTTAC GGTTCCATAG AAACTTGAAA AAGTTGAATG GCCCTTTGGA AAAATACATC
    TATATAATGG GAATACAAGA AAGAAATGAG AAATTGTTTT ACAGAATACT GCAAGATGAT
    ATTGAAACTC TAATGCCAAT TGTGTATACA CCAACAGTTG GCCTTGCCTG CTCCCAGTAT
    GGACACATCT TTAGAAGACC TAAGGGATTA TTTATTTCAA TCTCAGACAG AGGTCATGTT
    AGATCAATTG TGGATAACTG GCCAGAAAAT CATGTTAAGG CTGTCGTGGT GACTGATGGA
    GAGAGAATTC TAGGTCTTGG AGATCTGGGT GTCTATGGAA TGGGAATTCC AGTAGGAAAA
    CTTTGTTTGT ATACAGCTTG TGCGGGAATA CGGCCTGATA GATGTCTGCC TGTGTGTATT
    GACGTAGGAA CTGATAATCC GGCCCTCTTA AAGGATCCAT TTTATATGGG TTTATATCAG
    AAAAGAGATC GATCACAGCG TTACGATGAC CTAATTGATG AGTTTATGAA GGCCATTACT
    GACAGATATG GACAGAACAC ACTTATTCAG TTTGAAGACT TTGGAAATCA TAATGCATTC
    AGGTTCTTGA GAAAATACCG AGAAAAATAC TGTACCTTCA ACGATGATAT TCAAGGGACG
    GCTGCGGTGG CTCTGGCCGG TCTGCTTGCC GCGCAAAAAG TCATTGGGAA ACCGATCTCA
    GATCATAGAA TCTTATTTCT TGGAGCAGGA GAGGCTGCTC TTGGAATTGC AAATCTGATA
    GTTATGGCTA TGGTTGAAAA TGGCCTCTCA GAAGAAGAGG CACGAAAGAA AATTTGGATG
    GTTGACAAGT TTGGTTTATT ATTTAAGGGG CGGAAAGCTA AAATAGAAAG TCATCAGGAA
    GCATTTGCTC ACTCAGCTCC AGAGAGCCTA CCTGATACTT TTGAAGAGGC AGTAAATATA
    CTTAAGCCTT CAGCTATAAT TGGAGTTGCA GGAGCTGGCC GACTTTTCAC TCCTGGTGTA
    ATCAAAGCAA TGGCTGAAAT CAATGAAAGG CCTATAATAT TTGCATTAAG TAATCCTACG
    GCACAGGCTG AGTGCACAGC TGAAGAAGCA TACACGCTTA CAGAGGGAAG GTGTTTGTTT
    GCCAGTGGCA GTCCGTTTGA GCCAGTGAAA CTCAGTGATG GGCAAGTCTT TACACCAGGC
    CAAGGAAACA ATGTATATAT TTTTCCAGGT GTGGCTTTAG CTGTTATTCT CTGTAACACC
    CGGCATATTA GTGACCATGT GTTCCTGGAA GCTGCAAAGG CTCTGACAAG TCAGTTGACA
    GATAAAGAGC TGGCTCAGGG GAGACTTTAC CCACCACTTT CTAATATTCA GGAAGTTTCT
    ATTAACATTG CTATTAAAGT TACAGAATAC CTGTATGCTA ATAAAATGGC TTTCCGATAC
    CCAGAACCTG AGGACAAGGC CAAATACATT AGAGAAAGAA TATGGCGAAG CGAGTACGAT
    TCCCTGCTGC CCGATGTGTA TGAGTGGCCA GAGGCTGCAG CGCACCCTCC CCCCATAACA
    GAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.