Me2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Me2
Entrez Gene ID	107029
Full Name	malic enzyme 2, NAD(+)-dependent, mitochondrial
Synonyms	AW120568,D030040L20Rik,NAD-ME
General protein information	Preferred Names malic enzyme 2, NAD(+)-dependent, mitochondrial Names NAD-dependent malic enzyme, mitochondrial
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 E2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006525496.3	XP_006525559.1	NAD-dependent malic enzyme, mitochondrial isoform X1
NM_145494.2	NP_663469.1	NAD-dependent malic enzyme, mitochondrial precursor
XM_006525496.4	XP_006525559.1	NAD-dependent malic enzyme, mitochondrial isoform X1
NM_145494.3	NP_663469.1	NAD-dependent malic enzyme, mitochondrial precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ME2	NP_001070282.1
Oryza sativa (rice)	Os01g0723400	NP_001044107.1
Homo sapiens (human)	ME2	NP_002387.1
Pan troglodytes (chimpanzee)	ME2	XP_001154897.1
	ME2	XP_001099883.2
Danio rerio (zebrafish)	LOC100537927	XP_003198629.1
Arabidopsis thaliana (thale cress)	NADP-ME3	NP_197960.1
Arabidopsis thaliana (thale cress)	NADP-ME2	NP_196728.1
Mus musculus (house mouse)	Me2	NP_663469.1
Rattus norvegicus (Norway rat)	Me2	NP_001100846.2
Danio rerio (zebrafish)	me2	NP_001003627.2
Canis lupus familiaris (dog)	ME2	XP_005615454.1
Xenopus tropicalis (tropical clawed frog)	me2	NP_001015690.1

Gene Ontology
Bibliography

Related articles in PubMed

Genomic deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer.
Dey P, Baddour J, Muller F, Wu CC, Wang H, Liao WT, Lan Z, Chen A, Gutschner T, Kang Y, Fleming J, Satani N, Zhao D, Achreja A, Yang L, Lee J, Chang E, Genovese G, Viale A, Ying H, Draetta G, Maitra A, Wang YA, Nagrath D, DePinho RA
Nature542(7639)119-123(2017 02)

Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence.
Jiang P, Du W, Mancuso A, Wellen KE, Yang X
Nature493(7434)689-93(2013 Jan)

Identification of anchor genes during kidney development defines ontological relationships, molecular subcompartments and regulatory pathways.
Thiagarajan RD, Georgas KM, Rumballe BA, Lesieur E, Chiu HS, Taylor D, Tang DT, Grimmond SM, Little MH
PloS one6(2)e17286(2011 Feb)

Mitochondrial malic enzyme (ME2) in pancreatic islets of the human, rat and mouse and clonal insulinoma cells.
MacDonald MJ, Longacre MJ, Kendrick MA
Archives of biochemistry and biophysics488(2)100-4(2009 Aug)

The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesis.
Sansom SN, Griffiths DS, Faedo A, Kleinjan DJ, Ruan Y, Smith J, van Heyningen V, Rubenstein JL, Livesey FJ
PLoS genetics5(6)e1000511(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer
Title: Genomic deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer.

p53 represses the expression of the tricarboxylic-acid-cycle-associated malic enzymes ME1 and ME2 in human and mouse cells
Title: Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence.

Me2 activity in Islet of Langerhans cells was measured by a spectrophotometric enzyme assay by utilizing the distinct kinetic properties of Me2.
Title: Mitochondrial malic enzyme (ME2) in pancreatic islets of the human, rat and mouse and clonal insulinoma cells.

The following Me2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Me2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu17466	NM_145494.3 Latest version!	Mus musculus malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00
OMu54290	XM_006525496.4 Latest version!	Mus musculus malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu54290	XM_006525496.3	Mus musculus malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu17466	NM_145494.2	Mus musculus malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Me2 ( NM_145494.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663469.1 Mus musculus malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), mRNA.

Price & Availability↑

CloneID	OMu17466
Clone ID Related Accession (Same CDS sequence)	NM_145494.2 , NM_145494.3
Accession Version	NM_145494.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Mus musculus(house mouse)
Product	NAD-dependent malic enzyme, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC132418.3. On Nov 17, 2006 this sequence version replaced NM_145494.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC004709.1, AK043103.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTTTTCCC GGCTAAGAGC TGTTACCACT CCTTGTACCT TGACCTGCCG CCGAGTGCAC 
CTGAAAGAGA AGGGCAAGCC ACTCATGTTG AATCCAAGAA CAAACAAGGG AATGGCGTTT 
ACGTTACAAG AGCGACAAAT GCTTGGTCTT CAAGGACTTT TACCTCCTAA AATAGAGACA 
CAGGATATTC AAGCCTTACG ATTCCATAGA AACTTGAAAA AAATGACTAG CCCTTTGGAA 
AAATACATTT ATATAATGGG CATCCAAGAA AGAAATGAGA AACTGTTCTA TAGAATCCTG 
CAGGATGATA TTGAAAGTCT GATGCCGATT GTGTACACGC CAACAGTTGG TCTTGCCTGC 
TGTCAATATG GGCACATCTT TAGGAGACCT AAGGGATTGT TTATTTCAAT CTCAGACAGA 
GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTTGTGGTG 
ACTGATGGAG AGAGGATCCT GGGCCTTGGC GATCTGGGTG TCTATGGAAT GGGGATTCCA 
GTAGGGAAGC TGTGCCTGTA TACAGCCTGT GCAGGGATAC AGCCCGAGAA GTGCCTGCCC 
GTGTGCATCG ACGTGGGCAC TGATAACATG GCACTATTAA AAGATCCGTT TTATATGGGC 
TTGTATCAGA AGCGAGATCG TTCCCAGCTC TATGATGATC TGATGGACGA GTTTATGAAG 
GCGATTACTG ACAGATACGG CCGGAACACA CTCATTCAGT TTGAAGACTT TGGAAATCAT 
AATGCATTCA GGTTCTTAAG AAAATATCAA CAAAAATACT GTACCTTCAA TGATGACATT 
CAAGGGACAG CTGCAGTTGC TCTGTCTGGT CTTCTTGCGG CCCAAAGAGT TATTAATAAA 
CCAGTCTCAG AACACAAAAT CTTATTTCTT GGAGCAGGAG AAGCTGCACT TGGAATTGCC 
AATCTCATAG TTCTATCAAT GGTGGAAAGT GGTCTCTCAG AAGAGGAGGC GCAGAGAAAG 
ATCTGGATGT TTGACAAGAG TGGCTTGCTG GTTAAGGGGC GGACTGCTAG CATAGATAGC 
AACCAGGAAC CATATGCCCA TGCGGCCCCA GAGAGCATAC CTGCGACTTT TGAAGATGCA 
GTAAATAAAC TTAAGCCTTC AGTTATAATT GGAGTGGCAG GTGCCGGCCC GCTTTTTACT 
CACGGTGTAA TCAAAGCCAT GGCCTCTATC AACGAGAGAC CTATAATATT TGCTCTAAGT 
AATCCTACAG CACAGGCCGA GTGCACAGCC GAGGACGCGT ACACGCTCAC AGAGGGCAGG 
TGTCTGTTTG CCAGTGGCAG CCCATTTGAG CCAGTGAAAC TCCAGGATGG ACGAGTCTTC 
ACCCCAGGCC AAGGAAACAA TGCATATATT TTCCCAGGTG TGGCTTTAGC TGTTATTCTC 
TGTGAAGCCC GACACATCAG TGACACCGTT TTCTTAGAAG CTGCAAAGGC GCTGACGACT 
CAGTTGACAG ATGCAGAGTT AGCCCAAGGG AGGCTTTACC CGTCGCTGGC TAATATTCAG 
GAAGTTTCTG CTAACATAGC TATTAAACTT GCAGAGTACC TGTACGCTAA TAAAATGGCC 
TTCCGGTACC CAGAACCCGA GGACAAGGCC AGATACGTGA GAGAGAGAAT ATGGCGGAGC 
AACTACGTTT CCCTGCTGCC GGACGTGTAT GACTGGCCAG AGTCTTCACT GACACCTCCT 
CAGATCACAG AAGAGAAGCT TCCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663469.1
CDS	100..1869
Translation	MFSRLRAVTTPCTLTCRRVHLKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACCQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIQPEKCLPVCIDVGTDNMALLKDPFYMGLYQKRDRSQLYDDLMDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYQQKYCTFNDDIQGTAAVALSGLLAAQRVINKPVSEHKILFLGAGEAALGIANLIVLSMVESGLSEEEAQRKIWMFDKSGLLVKGRTASIDSNQEPYAHAAPESIPATFEDAVNKLKPSVIIGVAGAGPLFTHGVIKAMASINERPIIFALSNPTAQAECTAEDAYTLTEGRCLFASGSPFEPVKLQDGRVFTPGQGNNAYIFPGVALAVILCEARHISDTVFLEAAKALTTQLTDAELAQGRLYPSLANIQEVSANIAIKLAEYLYANKMAFRYPEPEDKARYVRERIWRSNYVSLLPDVYDWPESSLTPPQITEEKLPH

Target ORF information:

RefSeq Version	NM_145494.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145494.2

ORF Insert Sequence:

ATGTTTTCCC GGCTAAGAGC TGTTACCACT CCTTGTACCT TGACCTGCCG CCGAGTGCAC 
CTGAAAGAGA AGGGCAAGCC ACTCATGTTG AATCCAAGAA CAAACAAGGG AATGGCGTTT 
ACGTTACAAG AGCGACAAAT GCTTGGTCTT CAAGGACTTT TACCTCCTAA AATAGAGACA 
CAGGATATTC AAGCCTTACG ATTCCATAGA AACTTGAAAA AAATGACTAG CCCTTTGGAA 
AAATACATTT ATATAATGGG CATCCAAGAA AGAAATGAGA AACTGTTCTA TAGAATCCTG 
CAGGATGATA TTGAAAGTCT GATGCCGATT GTGTACACGC CAACAGTTGG TCTTGCCTGC 
TGTCAATATG GGCACATCTT TAGGAGACCT AAGGGATTGT TTATTTCAAT CTCAGACAGA 
GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTTGTGGTG 
ACTGATGGAG AGAGGATCCT GGGCCTTGGC GATCTGGGTG TCTATGGAAT GGGGATTCCA 
GTAGGGAAGC TGTGCCTGTA TACAGCCTGT GCAGGGATAC AGCCCGAGAA GTGCCTGCCC 
GTGTGCATCG ACGTGGGCAC TGATAACATG GCACTATTAA AAGATCCGTT TTATATGGGC 
TTGTATCAGA AGCGAGATCG TTCCCAGCTC TATGATGATC TGATGGACGA GTTTATGAAG 
GCGATTACTG ACAGATACGG CCGGAACACA CTCATTCAGT TTGAAGACTT TGGAAATCAT 
AATGCATTCA GGTTCTTAAG AAAATATCAA CAAAAATACT GTACCTTCAA TGATGACATT 
CAAGGGACAG CTGCAGTTGC TCTGTCTGGT CTTCTTGCGG CCCAAAGAGT TATTAATAAA 
CCAGTCTCAG AACACAAAAT CTTATTTCTT GGAGCAGGAG AAGCTGCACT TGGAATTGCC 
AATCTCATAG TTCTATCAAT GGTGGAAAGT GGTCTCTCAG AAGAGGAGGC GCAGAGAAAG 
ATCTGGATGT TTGACAAGAG TGGCTTGCTG GTTAAGGGGC GGACTGCTAG CATAGATAGC 
AACCAGGAAC CATATGCCCA TGCGGCCCCA GAGAGCATAC CTGCGACTTT TGAAGATGCA 
GTAAATAAAC TTAAGCCTTC AGTTATAATT GGAGTGGCAG GTGCCGGCCC GCTTTTTACT 
CACGGTGTAA TCAAAGCCAT GGCCTCTATC AACGAGAGAC CTATAATATT TGCTCTAAGT 
AATCCTACAG CACAGGCCGA GTGCACAGCC GAGGACGCGT ACACGCTCAC AGAGGGCAGG 
TGTCTGTTTG CCAGTGGCAG CCCATTTGAG CCAGTGAAAC TCCAGGATGG ACGAGTCTTC 
ACCCCAGGCC AAGGAAACAA TGCATATATT TTCCCAGGTG TGGCTTTAGC TGTTATTCTC 
TGTGAAGCCC GACACATCAG TGACACCGTT TTCTTAGAAG CTGCAAAGGC GCTGACGACT 
CAGTTGACAG ATGCAGAGTT AGCCCAAGGG AGGCTTTACC CGTCGCTGGC TAATATTCAG 
GAAGTTTCTG CTAACATAGC TATTAAACTT GCAGAGTACC TGTACGCTAA TAAAATGGCC 
TTCCGGTACC CAGAACCCGA GGACAAGGCC AGATACGTGA GAGAGAGAAT ATGGCGGAGC 
AACTACGTTT CCCTGCTGCC GGACGTGTAT GACTGGCCAG AGTCTTCACT GACACCTCCT 
CAGATCACAG AAGAGAAGCT TCCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Me2 ( NM_145494.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663469.1 Mus musculus malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu17466
Clone ID Related Accession (Same CDS sequence)	NM_145494.2 , NM_145494.3
Accession Version	NM_145494.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	NAD-dependent malic enzyme, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132418.3. On Jul 21, 2018 this sequence version replaced NM_145494.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK050933.1, AK156403.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTTTCCC GGCTAAGAGC TGTTACCACT CCTTGTACCT TGACCTGCCG CCGAGTGCAC 
CTGAAAGAGA AGGGCAAGCC ACTCATGTTG AATCCAAGAA CAAACAAGGG AATGGCGTTT 
ACGTTACAAG AGCGACAAAT GCTTGGTCTT CAAGGACTTT TACCTCCTAA AATAGAGACA 
CAGGATATTC AAGCCTTACG ATTCCATAGA AACTTGAAAA AAATGACTAG CCCTTTGGAA 
AAATACATTT ATATAATGGG CATCCAAGAA AGAAATGAGA AACTGTTCTA TAGAATCCTG 
CAGGATGATA TTGAAAGTCT GATGCCGATT GTGTACACGC CAACAGTTGG TCTTGCCTGC 
TGTCAATATG GGCACATCTT TAGGAGACCT AAGGGATTGT TTATTTCAAT CTCAGACAGA 
GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTTGTGGTG 
ACTGATGGAG AGAGGATCCT GGGCCTTGGC GATCTGGGTG TCTATGGAAT GGGGATTCCA 
GTAGGGAAGC TGTGCCTGTA TACAGCCTGT GCAGGGATAC AGCCCGAGAA GTGCCTGCCC 
GTGTGCATCG ACGTGGGCAC TGATAACATG GCACTATTAA AAGATCCGTT TTATATGGGC 
TTGTATCAGA AGCGAGATCG TTCCCAGCTC TATGATGATC TGATGGACGA GTTTATGAAG 
GCGATTACTG ACAGATACGG CCGGAACACA CTCATTCAGT TTGAAGACTT TGGAAATCAT 
AATGCATTCA GGTTCTTAAG AAAATATCAA CAAAAATACT GTACCTTCAA TGATGACATT 
CAAGGGACAG CTGCAGTTGC TCTGTCTGGT CTTCTTGCGG CCCAAAGAGT TATTAATAAA 
CCAGTCTCAG AACACAAAAT CTTATTTCTT GGAGCAGGAG AAGCTGCACT TGGAATTGCC 
AATCTCATAG TTCTATCAAT GGTGGAAAGT GGTCTCTCAG AAGAGGAGGC GCAGAGAAAG 
ATCTGGATGT TTGACAAGAG TGGCTTGCTG GTTAAGGGGC GGACTGCTAG CATAGATAGC 
AACCAGGAAC CATATGCCCA TGCGGCCCCA GAGAGCATAC CTGCGACTTT TGAAGATGCA 
GTAAATAAAC TTAAGCCTTC AGTTATAATT GGAGTGGCAG GTGCCGGCCC GCTTTTTACT 
CACGGTGTAA TCAAAGCCAT GGCCTCTATC AACGAGAGAC CTATAATATT TGCTCTAAGT 
AATCCTACAG CACAGGCCGA GTGCACAGCC GAGGACGCGT ACACGCTCAC AGAGGGCAGG 
TGTCTGTTTG CCAGTGGCAG CCCATTTGAG CCAGTGAAAC TCCAGGATGG ACGAGTCTTC 
ACCCCAGGCC AAGGAAACAA TGCATATATT TTCCCAGGTG TGGCTTTAGC TGTTATTCTC 
TGTGAAGCCC GACACATCAG TGACACCGTT TTCTTAGAAG CTGCAAAGGC GCTGACGACT 
CAGTTGACAG ATGCAGAGTT AGCCCAAGGG AGGCTTTACC CGTCGCTGGC TAATATTCAG 
GAAGTTTCTG CTAACATAGC TATTAAACTT GCAGAGTACC TGTACGCTAA TAAAATGGCC 
TTCCGGTACC CAGAACCCGA GGACAAGGCC AGATACGTGA GAGAGAGAAT ATGGCGGAGC 
AACTACGTTT CCCTGCTGCC GGACGTGTAT GACTGGCCAG AGTCTTCACT GACACCTCCT 
CAGATCACAG AAGAGAAGCT TCCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663469.1
CDS	146..1915
Translation	MFSRLRAVTTPCTLTCRRVHLKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACCQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIQPEKCLPVCIDVGTDNMALLKDPFYMGLYQKRDRSQLYDDLMDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYQQKYCTFNDDIQGTAAVALSGLLAAQRVINKPVSEHKILFLGAGEAALGIANLIVLSMVESGLSEEEAQRKIWMFDKSGLLVKGRTASIDSNQEPYAHAAPESIPATFEDAVNKLKPSVIIGVAGAGPLFTHGVIKAMASINERPIIFALSNPTAQAECTAEDAYTLTEGRCLFASGSPFEPVKLQDGRVFTPGQGNNAYIFPGVALAVILCEARHISDTVFLEAAKALTTQLTDAELAQGRLYPSLANIQEVSANIAIKLAEYLYANKMAFRYPEPEDKARYVRERIWRSNYVSLLPDVYDWPESSLTPPQITEEKLPH

Target ORF information:

RefSeq Version	NM_145494.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145494.3

ORF Insert Sequence:

ATGTTTTCCC GGCTAAGAGC TGTTACCACT CCTTGTACCT TGACCTGCCG CCGAGTGCAC 
CTGAAAGAGA AGGGCAAGCC ACTCATGTTG AATCCAAGAA CAAACAAGGG AATGGCGTTT 
ACGTTACAAG AGCGACAAAT GCTTGGTCTT CAAGGACTTT TACCTCCTAA AATAGAGACA 
CAGGATATTC AAGCCTTACG ATTCCATAGA AACTTGAAAA AAATGACTAG CCCTTTGGAA 
AAATACATTT ATATAATGGG CATCCAAGAA AGAAATGAGA AACTGTTCTA TAGAATCCTG 
CAGGATGATA TTGAAAGTCT GATGCCGATT GTGTACACGC CAACAGTTGG TCTTGCCTGC 
TGTCAATATG GGCACATCTT TAGGAGACCT AAGGGATTGT TTATTTCAAT CTCAGACAGA 
GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTTGTGGTG 
ACTGATGGAG AGAGGATCCT GGGCCTTGGC GATCTGGGTG TCTATGGAAT GGGGATTCCA 
GTAGGGAAGC TGTGCCTGTA TACAGCCTGT GCAGGGATAC AGCCCGAGAA GTGCCTGCCC 
GTGTGCATCG ACGTGGGCAC TGATAACATG GCACTATTAA AAGATCCGTT TTATATGGGC 
TTGTATCAGA AGCGAGATCG TTCCCAGCTC TATGATGATC TGATGGACGA GTTTATGAAG 
GCGATTACTG ACAGATACGG CCGGAACACA CTCATTCAGT TTGAAGACTT TGGAAATCAT 
AATGCATTCA GGTTCTTAAG AAAATATCAA CAAAAATACT GTACCTTCAA TGATGACATT 
CAAGGGACAG CTGCAGTTGC TCTGTCTGGT CTTCTTGCGG CCCAAAGAGT TATTAATAAA 
CCAGTCTCAG AACACAAAAT CTTATTTCTT GGAGCAGGAG AAGCTGCACT TGGAATTGCC 
AATCTCATAG TTCTATCAAT GGTGGAAAGT GGTCTCTCAG AAGAGGAGGC GCAGAGAAAG 
ATCTGGATGT TTGACAAGAG TGGCTTGCTG GTTAAGGGGC GGACTGCTAG CATAGATAGC 
AACCAGGAAC CATATGCCCA TGCGGCCCCA GAGAGCATAC CTGCGACTTT TGAAGATGCA 
GTAAATAAAC TTAAGCCTTC AGTTATAATT GGAGTGGCAG GTGCCGGCCC GCTTTTTACT 
CACGGTGTAA TCAAAGCCAT GGCCTCTATC AACGAGAGAC CTATAATATT TGCTCTAAGT 
AATCCTACAG CACAGGCCGA GTGCACAGCC GAGGACGCGT ACACGCTCAC AGAGGGCAGG 
TGTCTGTTTG CCAGTGGCAG CCCATTTGAG CCAGTGAAAC TCCAGGATGG ACGAGTCTTC 
ACCCCAGGCC AAGGAAACAA TGCATATATT TTCCCAGGTG TGGCTTTAGC TGTTATTCTC 
TGTGAAGCCC GACACATCAG TGACACCGTT TTCTTAGAAG CTGCAAAGGC GCTGACGACT 
CAGTTGACAG ATGCAGAGTT AGCCCAAGGG AGGCTTTACC CGTCGCTGGC TAATATTCAG 
GAAGTTTCTG CTAACATAGC TATTAAACTT GCAGAGTACC TGTACGCTAA TAAAATGGCC 
TTCCGGTACC CAGAACCCGA GGACAAGGCC AGATACGTGA GAGAGAGAAT ATGGCGGAGC 
AACTACGTTT CCCTGCTGCC GGACGTGTAT GACTGGCCAG AGTCTTCACT GACACCTCCT 
CAGATCACAG AAGAGAAGCT TCCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Me2 ( XM_006525496.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525559.1 Mus musculus malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54290
Clone ID Related Accession (Same CDS sequence)	XM_006525496.3 , XM_006525496.4
Accession Version	XM_006525496.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	NAD-dependent malic enzyme, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525496.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAACT GGGGAGAGAA GATGTTTTCC CGGCTAAGAG CTGTTACCAC TCCTTGTACC 
TTGACCTGCC GCCGAGTGCA CCTGAAAGAG AAGGGCAAGC CACTCATGTT GAATCCAAGA 
ACAAACAAGG GAATGGCGTT TACGTTACAA GAGCGACAAA TGCTTGGTCT TCAAGGACTT 
TTACCTCCTA AAATAGAGAC ACAGGATATT CAAGCCTTAC GATTCCATAG AAACTTGAAA 
AAAATGACTA GCCCTTTGGA AAAATACATT TATATAATGG GCATCCAAGA AAGAAATGAG 
AAACTGTTCT ATAGAATCCT GCAGGATGAT ATTGAAAGTC TGATGCCGAT TGTGTACACG 
CCAACAGTTG GTCTTGCCTG CTGTCAATAT GGGCACATCT TTAGGAGACC TAAGGGATTG 
TTTATTTCAA TCTCAGACAG AGGTCATGTT AGATCAATTG TGGATAACTG GCCAGAAAAT 
CATGTTAAGG CTGTTGTGGT GACTGATGGA GAGAGGATCC TGGGCCTTGG CGATCTGGGT 
GTCTATGGAA TGGGGATTCC AGTAGGGAAG CTGTGCCTGT ATACAGCCTG TGCAGGGATA 
CAGCCCGAGA AGTGCCTGCC CGTGTGCATC GACGTGGGCA CTGATAACAT GGCACTATTA 
AAAGATCCGT TTTATATGGG CTTGTATCAG AAGCGAGATC GTTCCCAGCT CTATGATGAT 
CTGATGGACG AGTTTATGAA GGCGATTACT GACAGATACG GCCGGAACAC ACTCATTCAG 
TTTGAAGACT TTGGAAATCA TAATGCATTC AGGTTCTTAA GAAAATATCA ACAAAAATAC 
TGTACCTTCA ATGATGACAT TCAAGGGACA GCTGCAGTTG CTCTGTCTGG TCTTCTTGCG 
GCCCAAAGAG TTATTAATAA ACCAGTCTCA GAACACAAAA TCTTATTTCT TGGAGCAGGA 
GAAGCTGCAC TTGGAATTGC CAATCTCATA GTTCTATCAA TGGTGGAAAG TGGTCTCTCA 
GAAGAGGAGG CGCAGAGAAA GATCTGGATG TTTGACAAGA GTGGCTTGCT GGTTAAGGGG 
CGGACTGCTA GCATAGATAG CAACCAGGAA CCATATGCCC ATGCGGCCCC AGAGAGCATA 
CCTGCGACTT TTGAAGATGC AGTAAATAAA CTTAAGCCTT CAGTTATAAT TGGAGTGGCA 
GGTGCCGGCC CGCTTTTTAC TCACGGTGTA ATCAAAGCCA TGGCCTCTAT CAACGAGAGA 
CCTATAATAT TTGCTCTAAG TAATCCTACA GCACAGGCCG AGTGCACAGC CGAGGACGCG 
TACACGCTCA CAGAGGGCAG GTGTCTGTTT GCCAGTGGCA GCCCATTTGA GCCAGTGAAA 
CTCCAGGATG GACGAGTCTT CACCCCAGGC CAAGGAAACA ATGCATATAT TTTCCCAGGT 
GTGGCTTTAG CTGTTATTCT CTGTGAAGCC CGACACATCA GTGACACCGT TTTCTTAGAA 
GCTGCAAAGG CGCTGACGAC TCAGTTGACA GATGCAGAGT TAGCCCAAGG GAGGCTTTAC 
CCGTCGCTGG CTAATATTCA GGAAGTTTCT GCTAACATAG CTATTAAACT TGCAGAGTAC 
CTGTACGCTA ATAAAATGGC CTTCCGGTAC CCAGAACCCG AGGACAAGGC CAGATACGTG 
AGAGAGAGAA TATGGCGGAG CAACTACGTT TCCCTGCTGC CGGACGTGTA TGACTGGCCA 
GAGTCTTCAC TGACACCTCC TCAGATCACA GAAGAGAAGC TTCCCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525559.1
CDS	377..2167
Translation	MRNWGEKMFSRLRAVTTPCTLTCRRVHLKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACCQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIQPEKCLPVCIDVGTDNMALLKDPFYMGLYQKRDRSQLYDDLMDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYQQKYCTFNDDIQGTAAVALSGLLAAQRVINKPVSEHKILFLGAGEAALGIANLIVLSMVESGLSEEEAQRKIWMFDKSGLLVKGRTASIDSNQEPYAHAAPESIPATFEDAVNKLKPSVIIGVAGAGPLFTHGVIKAMASINERPIIFALSNPTAQAECTAEDAYTLTEGRCLFASGSPFEPVKLQDGRVFTPGQGNNAYIFPGVALAVILCEARHISDTVFLEAAKALTTQLTDAELAQGRLYPSLANIQEVSANIAIKLAEYLYANKMAFRYPEPEDKARYVRERIWRSNYVSLLPDVYDWPESSLTPPQITEEKLPH

Target ORF information:

RefSeq Version	XM_006525496.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525496.3

ORF Insert Sequence:

ATGCGAAACT GGGGAGAGAA GATGTTTTCC CGGCTAAGAG CTGTTACCAC TCCTTGTACC 
TTGACCTGCC GCCGAGTGCA CCTGAAAGAG AAGGGCAAGC CACTCATGTT GAATCCAAGA 
ACAAACAAGG GAATGGCGTT TACGTTACAA GAGCGACAAA TGCTTGGTCT TCAAGGACTT 
TTACCTCCTA AAATAGAGAC ACAGGATATT CAAGCCTTAC GATTCCATAG AAACTTGAAA 
AAAATGACTA GCCCTTTGGA AAAATACATT TATATAATGG GCATCCAAGA AAGAAATGAG 
AAACTGTTCT ATAGAATCCT GCAGGATGAT ATTGAAAGTC TGATGCCGAT TGTGTACACG 
CCAACAGTTG GTCTTGCCTG CTGTCAATAT GGGCACATCT TTAGGAGACC TAAGGGATTG 
TTTATTTCAA TCTCAGACAG AGGTCATGTT AGATCAATTG TGGATAACTG GCCAGAAAAT 
CATGTTAAGG CTGTTGTGGT GACTGATGGA GAGAGGATCC TGGGCCTTGG CGATCTGGGT 
GTCTATGGAA TGGGGATTCC AGTAGGGAAG CTGTGCCTGT ATACAGCCTG TGCAGGGATA 
CAGCCCGAGA AGTGCCTGCC CGTGTGCATC GACGTGGGCA CTGATAACAT GGCACTATTA 
AAAGATCCGT TTTATATGGG CTTGTATCAG AAGCGAGATC GTTCCCAGCT CTATGATGAT 
CTGATGGACG AGTTTATGAA GGCGATTACT GACAGATACG GCCGGAACAC ACTCATTCAG 
TTTGAAGACT TTGGAAATCA TAATGCATTC AGGTTCTTAA GAAAATATCA ACAAAAATAC 
TGTACCTTCA ATGATGACAT TCAAGGGACA GCTGCAGTTG CTCTGTCTGG TCTTCTTGCG 
GCCCAAAGAG TTATTAATAA ACCAGTCTCA GAACACAAAA TCTTATTTCT TGGAGCAGGA 
GAAGCTGCAC TTGGAATTGC CAATCTCATA GTTCTATCAA TGGTGGAAAG TGGTCTCTCA 
GAAGAGGAGG CGCAGAGAAA GATCTGGATG TTTGACAAGA GTGGCTTGCT GGTTAAGGGG 
CGGACTGCTA GCATAGATAG CAACCAGGAA CCATATGCCC ATGCGGCCCC AGAGAGCATA 
CCTGCGACTT TTGAAGATGC AGTAAATAAA CTTAAGCCTT CAGTTATAAT TGGAGTGGCA 
GGTGCCGGCC CGCTTTTTAC TCACGGTGTA ATCAAAGCCA TGGCCTCTAT CAACGAGAGA 
CCTATAATAT TTGCTCTAAG TAATCCTACA GCACAGGCCG AGTGCACAGC CGAGGACGCG 
TACACGCTCA CAGAGGGCAG GTGTCTGTTT GCCAGTGGCA GCCCATTTGA GCCAGTGAAA 
CTCCAGGATG GACGAGTCTT CACCCCAGGC CAAGGAAACA ATGCATATAT TTTCCCAGGT 
GTGGCTTTAG CTGTTATTCT CTGTGAAGCC CGACACATCA GTGACACCGT TTTCTTAGAA 
GCTGCAAAGG CGCTGACGAC TCAGTTGACA GATGCAGAGT TAGCCCAAGG GAGGCTTTAC 
CCGTCGCTGG CTAATATTCA GGAAGTTTCT GCTAACATAG CTATTAAACT TGCAGAGTAC 
CTGTACGCTA ATAAAATGGC CTTCCGGTAC CCAGAACCCG AGGACAAGGC CAGATACGTG 
AGAGAGAGAA TATGGCGGAG CAACTACGTT TCCCTGCTGC CGGACGTGTA TGACTGGCCA 
GAGTCTTCAC TGACACCTCC TCAGATCACA GAAGAGAAGC TTCCCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Me2 ( XM_006525496.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525559.1 Mus musculus malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), transcript variant X1, mRNA.

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CloneID	OMu54290
Clone ID Related Accession (Same CDS sequence)	XM_006525496.3 , XM_006525496.4
Accession Version	XM_006525496.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NAD-dependent malic enzyme, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525496.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAACT GGGGAGAGAA GATGTTTTCC CGGCTAAGAG CTGTTACCAC TCCTTGTACC 
TTGACCTGCC GCCGAGTGCA CCTGAAAGAG AAGGGCAAGC CACTCATGTT GAATCCAAGA 
ACAAACAAGG GAATGGCGTT TACGTTACAA GAGCGACAAA TGCTTGGTCT TCAAGGACTT 
TTACCTCCTA AAATAGAGAC ACAGGATATT CAAGCCTTAC GATTCCATAG AAACTTGAAA 
AAAATGACTA GCCCTTTGGA AAAATACATT TATATAATGG GCATCCAAGA AAGAAATGAG 
AAACTGTTCT ATAGAATCCT GCAGGATGAT ATTGAAAGTC TGATGCCGAT TGTGTACACG 
CCAACAGTTG GTCTTGCCTG CTGTCAATAT GGGCACATCT TTAGGAGACC TAAGGGATTG 
TTTATTTCAA TCTCAGACAG AGGTCATGTT AGATCAATTG TGGATAACTG GCCAGAAAAT 
CATGTTAAGG CTGTTGTGGT GACTGATGGA GAGAGGATCC TGGGCCTTGG CGATCTGGGT 
GTCTATGGAA TGGGGATTCC AGTAGGGAAG CTGTGCCTGT ATACAGCCTG TGCAGGGATA 
CAGCCCGAGA AGTGCCTGCC CGTGTGCATC GACGTGGGCA CTGATAACAT GGCACTATTA 
AAAGATCCGT TTTATATGGG CTTGTATCAG AAGCGAGATC GTTCCCAGCT CTATGATGAT 
CTGATGGACG AGTTTATGAA GGCGATTACT GACAGATACG GCCGGAACAC ACTCATTCAG 
TTTGAAGACT TTGGAAATCA TAATGCATTC AGGTTCTTAA GAAAATATCA ACAAAAATAC 
TGTACCTTCA ATGATGACAT TCAAGGGACA GCTGCAGTTG CTCTGTCTGG TCTTCTTGCG 
GCCCAAAGAG TTATTAATAA ACCAGTCTCA GAACACAAAA TCTTATTTCT TGGAGCAGGA 
GAAGCTGCAC TTGGAATTGC CAATCTCATA GTTCTATCAA TGGTGGAAAG TGGTCTCTCA 
GAAGAGGAGG CGCAGAGAAA GATCTGGATG TTTGACAAGA GTGGCTTGCT GGTTAAGGGG 
CGGACTGCTA GCATAGATAG CAACCAGGAA CCATATGCCC ATGCGGCCCC AGAGAGCATA 
CCTGCGACTT TTGAAGATGC AGTAAATAAA CTTAAGCCTT CAGTTATAAT TGGAGTGGCA 
GGTGCCGGCC CGCTTTTTAC TCACGGTGTA ATCAAAGCCA TGGCCTCTAT CAACGAGAGA 
CCTATAATAT TTGCTCTAAG TAATCCTACA GCACAGGCCG AGTGCACAGC CGAGGACGCG 
TACACGCTCA CAGAGGGCAG GTGTCTGTTT GCCAGTGGCA GCCCATTTGA GCCAGTGAAA 
CTCCAGGATG GACGAGTCTT CACCCCAGGC CAAGGAAACA ATGCATATAT TTTCCCAGGT 
GTGGCTTTAG CTGTTATTCT CTGTGAAGCC CGACACATCA GTGACACCGT TTTCTTAGAA 
GCTGCAAAGG CGCTGACGAC TCAGTTGACA GATGCAGAGT TAGCCCAAGG GAGGCTTTAC 
CCGTCGCTGG CTAATATTCA GGAAGTTTCT GCTAACATAG CTATTAAACT TGCAGAGTAC 
CTGTACGCTA ATAAAATGGC CTTCCGGTAC CCAGAACCCG AGGACAAGGC CAGATACGTG 
AGAGAGAGAA TATGGCGGAG CAACTACGTT TCCCTGCTGC CGGACGTGTA TGACTGGCCA 
GAGTCTTCAC TGACACCTCC TCAGATCACA GAAGAGAAGC TTCCCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525559.1
CDS	179..1969
Translation	MRNWGEKMFSRLRAVTTPCTLTCRRVHLKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACCQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIQPEKCLPVCIDVGTDNMALLKDPFYMGLYQKRDRSQLYDDLMDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYQQKYCTFNDDIQGTAAVALSGLLAAQRVINKPVSEHKILFLGAGEAALGIANLIVLSMVESGLSEEEAQRKIWMFDKSGLLVKGRTASIDSNQEPYAHAAPESIPATFEDAVNKLKPSVIIGVAGAGPLFTHGVIKAMASINERPIIFALSNPTAQAECTAEDAYTLTEGRCLFASGSPFEPVKLQDGRVFTPGQGNNAYIFPGVALAVILCEARHISDTVFLEAAKALTTQLTDAELAQGRLYPSLANIQEVSANIAIKLAEYLYANKMAFRYPEPEDKARYVRERIWRSNYVSLLPDVYDWPESSLTPPQITEEKLPH

Target ORF information:

RefSeq Version	XM_006525496.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525496.4

ORF Insert Sequence:

ATGCGAAACT GGGGAGAGAA GATGTTTTCC CGGCTAAGAG CTGTTACCAC TCCTTGTACC 
TTGACCTGCC GCCGAGTGCA CCTGAAAGAG AAGGGCAAGC CACTCATGTT GAATCCAAGA 
ACAAACAAGG GAATGGCGTT TACGTTACAA GAGCGACAAA TGCTTGGTCT TCAAGGACTT 
TTACCTCCTA AAATAGAGAC ACAGGATATT CAAGCCTTAC GATTCCATAG AAACTTGAAA 
AAAATGACTA GCCCTTTGGA AAAATACATT TATATAATGG GCATCCAAGA AAGAAATGAG 
AAACTGTTCT ATAGAATCCT GCAGGATGAT ATTGAAAGTC TGATGCCGAT TGTGTACACG 
CCAACAGTTG GTCTTGCCTG CTGTCAATAT GGGCACATCT TTAGGAGACC TAAGGGATTG 
TTTATTTCAA TCTCAGACAG AGGTCATGTT AGATCAATTG TGGATAACTG GCCAGAAAAT 
CATGTTAAGG CTGTTGTGGT GACTGATGGA GAGAGGATCC TGGGCCTTGG CGATCTGGGT 
GTCTATGGAA TGGGGATTCC AGTAGGGAAG CTGTGCCTGT ATACAGCCTG TGCAGGGATA 
CAGCCCGAGA AGTGCCTGCC CGTGTGCATC GACGTGGGCA CTGATAACAT GGCACTATTA 
AAAGATCCGT TTTATATGGG CTTGTATCAG AAGCGAGATC GTTCCCAGCT CTATGATGAT 
CTGATGGACG AGTTTATGAA GGCGATTACT GACAGATACG GCCGGAACAC ACTCATTCAG 
TTTGAAGACT TTGGAAATCA TAATGCATTC AGGTTCTTAA GAAAATATCA ACAAAAATAC 
TGTACCTTCA ATGATGACAT TCAAGGGACA GCTGCAGTTG CTCTGTCTGG TCTTCTTGCG 
GCCCAAAGAG TTATTAATAA ACCAGTCTCA GAACACAAAA TCTTATTTCT TGGAGCAGGA 
GAAGCTGCAC TTGGAATTGC CAATCTCATA GTTCTATCAA TGGTGGAAAG TGGTCTCTCA 
GAAGAGGAGG CGCAGAGAAA GATCTGGATG TTTGACAAGA GTGGCTTGCT GGTTAAGGGG 
CGGACTGCTA GCATAGATAG CAACCAGGAA CCATATGCCC ATGCGGCCCC AGAGAGCATA 
CCTGCGACTT TTGAAGATGC AGTAAATAAA CTTAAGCCTT CAGTTATAAT TGGAGTGGCA 
GGTGCCGGCC CGCTTTTTAC TCACGGTGTA ATCAAAGCCA TGGCCTCTAT CAACGAGAGA 
CCTATAATAT TTGCTCTAAG TAATCCTACA GCACAGGCCG AGTGCACAGC CGAGGACGCG 
TACACGCTCA CAGAGGGCAG GTGTCTGTTT GCCAGTGGCA GCCCATTTGA GCCAGTGAAA 
CTCCAGGATG GACGAGTCTT CACCCCAGGC CAAGGAAACA ATGCATATAT TTTCCCAGGT 
GTGGCTTTAG CTGTTATTCT CTGTGAAGCC CGACACATCA GTGACACCGT TTTCTTAGAA 
GCTGCAAAGG CGCTGACGAC TCAGTTGACA GATGCAGAGT TAGCCCAAGG GAGGCTTTAC 
CCGTCGCTGG CTAATATTCA GGAAGTTTCT GCTAACATAG CTATTAAACT TGCAGAGTAC 
CTGTACGCTA ATAAAATGGC CTTCCGGTAC CCAGAACCCG AGGACAAGGC CAGATACGTG 
AGAGAGAGAA TATGGCGGAG CAACTACGTT TCCCTGCTGC CGGACGTGTA TGACTGGCCA 
GAGTCTTCAC TGACACCTCC TCAGATCACA GAAGAGAAGC TTCCCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genomic deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer.
Nature542(7639)119-123(2017 02)
Dey P,Baddour J,Muller F,Wu CC,Wang H,Liao WT,Lan Z,Chen A,Gutschner T,Kang Y,Fleming J,Satani N,Zhao D,Achreja A,Yang L,Lee J,Chang E,Genovese G,Viale A,Ying H,Draetta G,Maitra A,Wang YA,Nagrath D,DePinho RA

Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence.
Nature493(7434)689-93(2013 Jan)
Jiang P,Du W,Mancuso A,Wellen KE,Yang X

Identification of anchor genes during kidney development defines ontological relationships, molecular subcompartments and regulatory pathways.
PloS one6(2)e17286(2011 Feb)
Thiagarajan RD,Georgas KM,Rumballe BA,Lesieur E,Chiu HS,Taylor D,Tang DT,Grimmond SM,Little MH

Mitochondrial malic enzyme (ME2) in pancreatic islets of the human, rat and mouse and clonal insulinoma cells.
Archives of biochemistry and biophysics488(2)100-4(2009 Aug)
MacDonald MJ,Longacre MJ,Kendrick MA

The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesis.
PLoS genetics5(6)e1000511(2009 Jun)
Sansom SN,Griffiths DS,Faedo A,Kleinjan DJ,Ruan Y,Smith J,van Heyningen V,Rubenstein JL,Livesey FJ