MARS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MARS1
Entrez Gene ID	4141
Full Name	methionyl-tRNA synthetase
Synonyms	CMT2U,ILFS2,ILLD,METRS,MRS,MTRNS,SPG70
General protein information	Preferred Names methionyl-tRNA synthetase Names methionine--tRNA ligase, cytoplasmic cytosolic methionyl-tRNA synthetase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.3
Summary	This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [provided by RefSeq, Jan 2011].
Disorder MIM:	156560
Disorder Html:	Interstitial lung and liver disease, 615486 (3); Charcot-Marie-Tooth disease, axonal, type 2U, 616280 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006719398.3	XP_006719461.1	methionine--tRNA ligase, cytoplasmic isoform X1
NM_004990.4	NP_004981.2	methionine--tRNA ligase, cytoplasmic
NM_004990.3	NP_004981.2	methionine--tRNA ligase, cytoplasmic
XM_006719398.4	XP_006719461.1	methionine--tRNA ligase, cytoplasmic isoform X1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0A09647g	XP_451421.1
Bos taurus (cattle)	MARS	NP_001033180.1
Mus musculus (house mouse)	Mars	NP_001165053.1
Rattus norvegicus (Norway rat)	Mars	NP_001121131.1
Schizosaccharomyces pombe (fission yeast)	rar1	NP_595586.1
Homo sapiens (human)	MARS	NP_004981.2
Pan troglodytes (chimpanzee)	MARS	XP_509164.2
	MARS	XP_001116063.1
Canis lupus familiaris (dog)	MARS	XP_531646.2
Gallus gallus (chicken)	MARS	NP_001244301.1
Danio rerio (zebrafish)	mars	NP_956370.1
Eremothecium gossypii	AGOS_AGR332C	NP_986998.1
Xenopus tropicalis (tropical clawed frog)	mars	NP_001017000.2
Drosophila melanogaster (fruit fly)	CG15100	NP_611382.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007891	XP_317601.4
Neurospora crassa	NCU07451	XP_964638.1
Saccharomyces cerevisiae (baker's yeast)	MES1	NP_011780.3
Magnaporthe oryzae (rice blast fungus)	MGG_01046	XP_003717896.1
Oryza sativa (rice)	Os10g0400100	NP_001064541.1

Gene Ontology
Bibliography

Related articles in PubMed

Assembly of Multi-tRNA Synthetase Complex via Heterotetrameric Glutathione Transferase-homology Domains.
Cho HY, Maeng SJ, Cho HJ, Choi YS, Chung JM, Lee S, Kim HK, Kim JH, Eom CY, Kim YG, Guo M, Jung HS, Kang BS, Kim S
The Journal of biological chemistry290(49)29313-28(2015 Dec)

Biallelic Mutations of Methionyl-tRNA Synthetase Cause a Specific Type of Pulmonary Alveolar Proteinosis Prevalent on R?union Island.
Hadchouel A, Wieland T, Griese M, Baruffini E, Lorenz-Depiereux B, Enaud L, Graf E, Dubus JC, Halioui-Louhaichi S, Coulomb A, Delacourt C, Eckstein G, Zarbock R, Schwarzmayr T, Cartault F, Meitinger T, Lodi T, de Blic J, Strom TM
American journal of human genetics96(5)826-31(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genotype-phenotype correlation analysis suggests most of the interstitial lung and liver disease (ILLD), mutations locate in the catalytic domain of MARS. ILLD and Charcot-Marie-Tooth disease, axonal, type 2U might have different disease mechanism.
Title: Mutations in methionyl-tRNA synthetase gene in a Chinese family with interstitial lung and liver disease, postnatal growth failure and anemia.

analysis of the heterotetrameric complex structure of the glutathione transferase (GST) domains shared among the four MSC components, methionyl-tRNA synthetase (MRS), glutaminyl-prolyl-tRNA synthetase (EPRS), AIMP2 and AIMP3
Title: Assembly of Multi-tRNA Synthetase Complex via Heterotetrameric Glutathione Transferase-homology Domains.

identification of a founder mutation in MARS led to the molecular definition of a specific type of pulmonary alveolar proteinosis and will enable carrier screening in the affected community and possibly open new treatment opportunities.
Title: Biallelic Mutations of Methionyl-tRNA Synthetase Cause a Specific Type of Pulmonary Alveolar Proteinosis Prevalent on R?union Island.

the association of rare MARS variant with late-onset autosomal dominant Charcot-Marie-Tooth neuropathy
Title: Rare variants in methionyl- and tyrosyl-tRNA synthetase genes in late-onset autosomal dominant Charcot-Marie-Tooth neuropathy.

The F370L and I523T mutations did not affect the association of MARS with the multisynthetase complex.
Title: Rare recessive loss-of-function methionyl-tRNA synthetase mutations presenting as a multi-organ phenotype.

The following MARS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MARS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21976	NM_004990.4 Latest version!	Homo sapiens methionyl-tRNA synthetase 1 (MARS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$454.50-$636.30 $909.00
OHu46556	XM_006719398.4 Latest version!	Homo sapiens methionyl-tRNA synthetase 1 (MARS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
View Old Accession Versions >>
OHu21976	NM_004990.3	Homo sapiens methionyl-tRNA synthetase (MARS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$454.50-$636.30 $909.00
OHu46556	XM_006719398.3	Homo sapiens methionyl-tRNA synthetase (MARS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MARS1 ( NM_004990.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004981.2 Homo sapiens methionyl-tRNA synthetase 1 (MARS1), mRNA.

Price & Availability↑

CloneID	OHu21976
Clone ID Related Accession (Same CDS sequence)	NM_004990.4 , NM_004990.3
Accession Version	NM_004990.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	methionine--tRNA ligase, cytoplasmic
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D84224.1 and BC011849.2. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_004990.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D84224.1, SRR3476690.762524.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262027.10/ ENSP00000262027.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGACTGT TCGTGAGTGA TGGCGTCCCG GGTTGCTTGC CGGTGCTGGC CGCCGCCGGG 
AGAGCCCGGG GCAGAGCAGA GGTGCTCATC AGCACTGTAG GCCCGGAAGA TTGTGTGGTC 
CCGTTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGCGGCAA CTACCTCTTC 
TCCACTAGTG CAATCTGCCG ATATTTTTTT TTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACTAACCAGT GGCTGGAATG GGAAGCGACA GAGCTGCAGC CAGCTTTGTC TGCTGCCCTG 
TACTATTTAG TGGTCCAAGG CAAGAAGGGG GAAGATGTTC TTGGTTCAGT GCGGAGAGCC 
CTGACTCACA TTGACCACAG CTTGAGTCGT CAGAACTGTC CTTTCCTGGC TGGGGAGACA 
GAATCTCTAG CCGACATTGT TTTGTGGGGA GCCCTATACC CATTACTGCA AGATCCCGCC 
TACCTCCCTG AGGAGCTGAG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TACCCAGGAA 
CCATGTCAGC GAGCTGCAGA GACTGTACTG AAACAGCAAG GTGTCCTGGC TCTCCGGCCT 
TACCTCCAAA AGCAGCCCCA GCCCAGCCCC GCTGAGGGAA GGGCTGTCAC CAATGAGCCT 
GAGGAGGAGG AGCTGGCTAC CCTATCTGAG GAGGAGATTG CTATGGCTGT TACTGCTTGG 
GAGAAGGGCC TAGAAAGTTT GCCCCCGCTG CGGCCCCAGC AGAATCCAGT GTTGCCTGTG 
GCTGGAGAAA GGAATGTGCT CATCACCAGT GCCCTCCCTT ACGTCAACAA TGTCCCCCAC 
CTTGGGAACA TCATTGGTTG TGTGCTCAGT GCCGATGTCT TTGCCAGGTA CTCTCGCCTC 
CGCCAGTGGA ACACCCTCTA TCTGTGTGGG ACAGATGAGT ATGGTACAGC AACAGAGACC 
AAGGCTCTGG AGGAGGGACT AACCCCCCAG GAGATCTGCG ACAAGTACCA CATCATCCAT 
GCTGACATCT ACCGCTGGTT TAACATTTCG TTTGATATTT TTGGTCGCAC CACCACTCCA 
CAGCAGACCA AAATCACCCA GGACATTTTC CAGCAGTTGC TGAAACGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT GCGATGTGAG CACTGTGCTC GCTTCCTGGC TGACCGCTTC 
GTGGAGGGCG TGTGTCCCTT CTGTGGCTAT GAGGAGGCTC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATCAATGC TGTCGAGCTT AAGAAGCCTC AGTGTAAAGT CTGCCGATCA 
TGCCCTGTGG TGCAGTCGAG CCAGCACCTG TTTCTGGACC TGCCTAAGCT GGAGAAGCGA 
CTGGAGGAGT GGTTGGGGAG GACATTGCCT GGCAGTGACT GGACACCCAA TGCCCAGTTT 
ATCACCCGTT CTTGGCTTCG GGATGGCCTC AAGCCACGCT GCATAACCCG AGACCTCAAA 
TGGGGAACCC CTGTACCCTT AGAAGGTTTT GAAGACAAGG TATTCTATGT CTGGTTTGAT 
GCCACTATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAGTGGGA GAGATGGTGG 
AAGAACCCAG AGCAAGTGGA CCTGTATCAG TTCATGGCCA AAGACAATGT TCCTTTCCAT 
AGCTTAGTCT TTCCTTGCTC AGCCCTAGGA GCTGAGGATA ACTATACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTACCT GAACTATGAG GATGGGAAAT TCTCTAAGAG CCGCGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACG GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA TTCGGCCTGA GGGCCAGGAC AGTGCTTTCT CCTGGACGGA CCTGCTGCTG 
AAGAATAATT CTGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCTAAGT TCTTTGGGGG CTATGTGCCT GAGATGGTGC TCACCCCTGA TGATCAGCGC 
CTGCTGGCCC ATGTCACCCT GGAGCTCCAG CACTATCACC AGCTACTTGA GAAGGTTCGG 
ATCCGGGATG CCTTGCGCAG TATCCTCACC ATATCTCGAC ATGGCAACCA ATATATTCAG 
GTGAATGAGC CCTGGAAGCG GATTAAAGGC AGTGAGGCTG ACAGGCAACG GGCAGGAACA 
GTGACTGGCT TGGCAGTGAA TATAGCTGCC TTGCTCTCTG TCATGCTTCA GCCTTACATG 
CCCACGGTTA GTGCCACAAT CCAGGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCTGACAA ACTTCCTGTG TACCTTACCA GCAGGACACC AGATTGGCAC AGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTTTAA GGCAGCGCTT TGGAGGGGGC 
CAGGCAAAAA CGTCCCCGAA GCCAGCAGTT GTAGAGACTG TTACAACAGC CAAGCCACAG 
CAGATACAAG CGCTGATGGA TGAAGTGACA AAACAAGGAA ACATTGTCCG AGAACTGAAA 
GCACAAAAGG CAGACAAGAA CGAGGTTGCT GCGGAGGTGG CGAAACTCTT GGATCTAAAG 
AAACAGTTGG CTGTAGCTGA GGGGAAACCC CCTGAAGCCC CTAAAGGCAA GAAGAAAAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004981.2
CDS	24..2726
Translation	MRLFVSDGVPGCLPVLAAAGRARGRAEVLISTVGPEDCVVPFLTRPKVPVLQLDSGNYLFSTSAICRYFFLLSGWEQDDLTNQWLEWEATELQPALSAALYYLVVQGKKGEDVLGSVRRALTHIDHSLSRQNCPFLAGETESLADIVLWGALYPLLQDPAYLPEELSALHSWFQTLSTQEPCQRAAETVLKQQGVLALRPYLQKQPQPSPAEGRAVTNEPEEEELATLSEEEIAMAVTAWEKGLESLPPLRPQQNPVLPVAGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKALEEGLTPQEICDKYHIIHADIYRWFNISFDIFGRTTTPQQTKITQDIFQQLLKRGFVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAVELKKPQCKVCRSCPVVQSSQHLFLDLPKLEKRLEEWLGRTLPGSDWTPNAQFITRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWERWWKNPEQVDLYQFMAKDNVPFHSLVFPCSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLLKNNSELLNNLGNFINRAGMFVSKFFGGYVPEMVLTPDDQRLLAHVTLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGSEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILLTNFLCTLPAGHQIGTVSPLFQKLENDQIESLRQRFGGGQAKTSPKPAVVETVTTAKPQQIQALMDEVTKQGNIVRELKAQKADKNEVAAEVAKLLDLKKQLAVAEGKPPEAPKGKKKK

Target ORF information:

RefSeq Version	NM_004990.4
Organism	Homo sapiens(human)
Definition	Homo sapiens methionyl-tRNA synthetase 1 (MARS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004990.4

ORF Insert Sequence:

ATGAGACTGT TCGTGAGTGA TGGCGTCCCG GGTTGCTTGC CGGTGCTGGC CGCCGCCGGG 
AGAGCCCGGG GCAGAGCAGA GGTGCTCATC AGCACTGTAG GCCCGGAAGA TTGTGTGGTC 
CCGTTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGCGGCAA CTACCTCTTC 
TCCACTAGTG CAATCTGCCG ATATTTTTTT TTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACTAACCAGT GGCTGGAATG GGAAGCGACA GAGCTGCAGC CAGCTTTGTC TGCTGCCCTG 
TACTATTTAG TGGTCCAAGG CAAGAAGGGG GAAGATGTTC TTGGTTCAGT GCGGAGAGCC 
CTGACTCACA TTGACCACAG CTTGAGTCGT CAGAACTGTC CTTTCCTGGC TGGGGAGACA 
GAATCTCTAG CCGACATTGT TTTGTGGGGA GCCCTATACC CATTACTGCA AGATCCCGCC 
TACCTCCCTG AGGAGCTGAG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TACCCAGGAA 
CCATGTCAGC GAGCTGCAGA GACTGTACTG AAACAGCAAG GTGTCCTGGC TCTCCGGCCT 
TACCTCCAAA AGCAGCCCCA GCCCAGCCCC GCTGAGGGAA GGGCTGTCAC CAATGAGCCT 
GAGGAGGAGG AGCTGGCTAC CCTATCTGAG GAGGAGATTG CTATGGCTGT TACTGCTTGG 
GAGAAGGGCC TAGAAAGTTT GCCCCCGCTG CGGCCCCAGC AGAATCCAGT GTTGCCTGTG 
GCTGGAGAAA GGAATGTGCT CATCACCAGT GCCCTCCCTT ACGTCAACAA TGTCCCCCAC 
CTTGGGAACA TCATTGGTTG TGTGCTCAGT GCCGATGTCT TTGCCAGGTA CTCTCGCCTC 
CGCCAGTGGA ACACCCTCTA TCTGTGTGGG ACAGATGAGT ATGGTACAGC AACAGAGACC 
AAGGCTCTGG AGGAGGGACT AACCCCCCAG GAGATCTGCG ACAAGTACCA CATCATCCAT 
GCTGACATCT ACCGCTGGTT TAACATTTCG TTTGATATTT TTGGTCGCAC CACCACTCCA 
CAGCAGACCA AAATCACCCA GGACATTTTC CAGCAGTTGC TGAAACGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT GCGATGTGAG CACTGTGCTC GCTTCCTGGC TGACCGCTTC 
GTGGAGGGCG TGTGTCCCTT CTGTGGCTAT GAGGAGGCTC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATCAATGC TGTCGAGCTT AAGAAGCCTC AGTGTAAAGT CTGCCGATCA 
TGCCCTGTGG TGCAGTCGAG CCAGCACCTG TTTCTGGACC TGCCTAAGCT GGAGAAGCGA 
CTGGAGGAGT GGTTGGGGAG GACATTGCCT GGCAGTGACT GGACACCCAA TGCCCAGTTT 
ATCACCCGTT CTTGGCTTCG GGATGGCCTC AAGCCACGCT GCATAACCCG AGACCTCAAA 
TGGGGAACCC CTGTACCCTT AGAAGGTTTT GAAGACAAGG TATTCTATGT CTGGTTTGAT 
GCCACTATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAGTGGGA GAGATGGTGG 
AAGAACCCAG AGCAAGTGGA CCTGTATCAG TTCATGGCCA AAGACAATGT TCCTTTCCAT 
AGCTTAGTCT TTCCTTGCTC AGCCCTAGGA GCTGAGGATA ACTATACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTACCT GAACTATGAG GATGGGAAAT TCTCTAAGAG CCGCGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACG GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA TTCGGCCTGA GGGCCAGGAC AGTGCTTTCT CCTGGACGGA CCTGCTGCTG 
AAGAATAATT CTGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCTAAGT TCTTTGGGGG CTATGTGCCT GAGATGGTGC TCACCCCTGA TGATCAGCGC 
CTGCTGGCCC ATGTCACCCT GGAGCTCCAG CACTATCACC AGCTACTTGA GAAGGTTCGG 
ATCCGGGATG CCTTGCGCAG TATCCTCACC ATATCTCGAC ATGGCAACCA ATATATTCAG 
GTGAATGAGC CCTGGAAGCG GATTAAAGGC AGTGAGGCTG ACAGGCAACG GGCAGGAACA 
GTGACTGGCT TGGCAGTGAA TATAGCTGCC TTGCTCTCTG TCATGCTTCA GCCTTACATG 
CCCACGGTTA GTGCCACAAT CCAGGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCTGACAA ACTTCCTGTG TACCTTACCA GCAGGACACC AGATTGGCAC AGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTTTAA GGCAGCGCTT TGGAGGGGGC 
CAGGCAAAAA CGTCCCCGAA GCCAGCAGTT GTAGAGACTG TTACAACAGC CAAGCCACAG 
CAGATACAAG CGCTGATGGA TGAAGTGACA AAACAAGGAA ACATTGTCCG AGAACTGAAA 
GCACAAAAGG CAGACAAGAA CGAGGTTGCT GCGGAGGTGG CGAAACTCTT GGATCTAAAG 
AAACAGTTGG CTGTAGCTGA GGGGAAACCC CCTGAAGCCC CTAAAGGCAA GAAGAAAAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MARS1 ( NM_004990.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004981.2 Homo sapiens methionyl-tRNA synthetase (MARS), mRNA.

Price & Availability↑

CloneID	OHu21976
Clone ID Related Accession (Same CDS sequence)	NM_004990.4 , NM_004990.3
Accession Version	NM_004990.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	methionine--tRNA ligase, cytoplasmic
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA489707.1, D84224.1 and BC011849.2. This sequence is a reference standard in the RefSeqGene project. On Jan 21, 2011 this sequence version replaced NM_004990.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011849.2, SRR1660805.177142.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGACTGT TCGTGAGTGA TGGCGTCCCG GGTTGCTTGC CGGTGCTGGC CGCCGCCGGG 
AGAGCCCGGG GCAGAGCAGA GGTGCTCATC AGCACTGTAG GCCCGGAAGA TTGTGTGGTC 
CCGTTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGCGGCAA CTACCTCTTC 
TCCACTAGTG CAATCTGCCG ATATTTTTTT TTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACTAACCAGT GGCTGGAATG GGAAGCGACA GAGCTGCAGC CAGCTTTGTC TGCTGCCCTG 
TACTATTTAG TGGTCCAAGG CAAGAAGGGG GAAGATGTTC TTGGTTCAGT GCGGAGAGCC 
CTGACTCACA TTGACCACAG CTTGAGTCGT CAGAACTGTC CTTTCCTGGC TGGGGAGACA 
GAATCTCTAG CCGACATTGT TTTGTGGGGA GCCCTATACC CATTACTGCA AGATCCCGCC 
TACCTCCCTG AGGAGCTGAG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TACCCAGGAA 
CCATGTCAGC GAGCTGCAGA GACTGTACTG AAACAGCAAG GTGTCCTGGC TCTCCGGCCT 
TACCTCCAAA AGCAGCCCCA GCCCAGCCCC GCTGAGGGAA GGGCTGTCAC CAATGAGCCT 
GAGGAGGAGG AGCTGGCTAC CCTATCTGAG GAGGAGATTG CTATGGCTGT TACTGCTTGG 
GAGAAGGGCC TAGAAAGTTT GCCCCCGCTG CGGCCCCAGC AGAATCCAGT GTTGCCTGTG 
GCTGGAGAAA GGAATGTGCT CATCACCAGT GCCCTCCCTT ACGTCAACAA TGTCCCCCAC 
CTTGGGAACA TCATTGGTTG TGTGCTCAGT GCCGATGTCT TTGCCAGGTA CTCTCGCCTC 
CGCCAGTGGA ACACCCTCTA TCTGTGTGGG ACAGATGAGT ATGGTACAGC AACAGAGACC 
AAGGCTCTGG AGGAGGGACT AACCCCCCAG GAGATCTGCG ACAAGTACCA CATCATCCAT 
GCTGACATCT ACCGCTGGTT TAACATTTCG TTTGATATTT TTGGTCGCAC CACCACTCCA 
CAGCAGACCA AAATCACCCA GGACATTTTC CAGCAGTTGC TGAAACGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT GCGATGTGAG CACTGTGCTC GCTTCCTGGC TGACCGCTTC 
GTGGAGGGCG TGTGTCCCTT CTGTGGCTAT GAGGAGGCTC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATCAATGC TGTCGAGCTT AAGAAGCCTC AGTGTAAAGT CTGCCGATCA 
TGCCCTGTGG TGCAGTCGAG CCAGCACCTG TTTCTGGACC TGCCTAAGCT GGAGAAGCGA 
CTGGAGGAGT GGTTGGGGAG GACATTGCCT GGCAGTGACT GGACACCCAA TGCCCAGTTT 
ATCACCCGTT CTTGGCTTCG GGATGGCCTC AAGCCACGCT GCATAACCCG AGACCTCAAA 
TGGGGAACCC CTGTACCCTT AGAAGGTTTT GAAGACAAGG TATTCTATGT CTGGTTTGAT 
GCCACTATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAGTGGGA GAGATGGTGG 
AAGAACCCAG AGCAAGTGGA CCTGTATCAG TTCATGGCCA AAGACAATGT TCCTTTCCAT 
AGCTTAGTCT TTCCTTGCTC AGCCCTAGGA GCTGAGGATA ACTATACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTACCT GAACTATGAG GATGGGAAAT TCTCTAAGAG CCGCGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACG GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA TTCGGCCTGA GGGCCAGGAC AGTGCTTTCT CCTGGACGGA CCTGCTGCTG 
AAGAATAATT CTGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCTAAGT TCTTTGGGGG CTATGTGCCT GAGATGGTGC TCACCCCTGA TGATCAGCGC 
CTGCTGGCCC ATGTCACCCT GGAGCTCCAG CACTATCACC AGCTACTTGA GAAGGTTCGG 
ATCCGGGATG CCTTGCGCAG TATCCTCACC ATATCTCGAC ATGGCAACCA ATATATTCAG 
GTGAATGAGC CCTGGAAGCG GATTAAAGGC AGTGAGGCTG ACAGGCAACG GGCAGGAACA 
GTGACTGGCT TGGCAGTGAA TATAGCTGCC TTGCTCTCTG TCATGCTTCA GCCTTACATG 
CCCACGGTTA GTGCCACAAT CCAGGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCTGACAA ACTTCCTGTG TACCTTACCA GCAGGACACC AGATTGGCAC AGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTTTAA GGCAGCGCTT TGGAGGGGGC 
CAGGCAAAAA CGTCCCCGAA GCCAGCAGTT GTAGAGACTG TTACAACAGC CAAGCCACAG 
CAGATACAAG CGCTGATGGA TGAAGTGACA AAACAAGGAA ACATTGTCCG AGAACTGAAA 
GCACAAAAGG CAGACAAGAA CGAGGTTGCT GCGGAGGTGG CGAAACTCTT GGATCTAAAG 
AAACAGTTGG CTGTAGCTGA GGGGAAACCC CCTGAAGCCC CTAAAGGCAA GAAGAAAAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004981.2
CDS	139..2841
Translation	MRLFVSDGVPGCLPVLAAAGRARGRAEVLISTVGPEDCVVPFLTRPKVPVLQLDSGNYLFSTSAICRYFFLLSGWEQDDLTNQWLEWEATELQPALSAALYYLVVQGKKGEDVLGSVRRALTHIDHSLSRQNCPFLAGETESLADIVLWGALYPLLQDPAYLPEELSALHSWFQTLSTQEPCQRAAETVLKQQGVLALRPYLQKQPQPSPAEGRAVTNEPEEEELATLSEEEIAMAVTAWEKGLESLPPLRPQQNPVLPVAGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKALEEGLTPQEICDKYHIIHADIYRWFNISFDIFGRTTTPQQTKITQDIFQQLLKRGFVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAVELKKPQCKVCRSCPVVQSSQHLFLDLPKLEKRLEEWLGRTLPGSDWTPNAQFITRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWERWWKNPEQVDLYQFMAKDNVPFHSLVFPCSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLLKNNSELLNNLGNFINRAGMFVSKFFGGYVPEMVLTPDDQRLLAHVTLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGSEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILLTNFLCTLPAGHQIGTVSPLFQKLENDQIESLRQRFGGGQAKTSPKPAVVETVTTAKPQQIQALMDEVTKQGNIVRELKAQKADKNEVAAEVAKLLDLKKQLAVAEGKPPEAPKGKKKK

Target ORF information:

RefSeq Version	NM_004990.3
Organism	Homo sapiens(human)
Definition	Homo sapiens methionyl-tRNA synthetase (MARS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004990.3

ORF Insert Sequence:

ATGAGACTGT TCGTGAGTGA TGGCGTCCCG GGTTGCTTGC CGGTGCTGGC CGCCGCCGGG 
AGAGCCCGGG GCAGAGCAGA GGTGCTCATC AGCACTGTAG GCCCGGAAGA TTGTGTGGTC 
CCGTTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGCGGCAA CTACCTCTTC 
TCCACTAGTG CAATCTGCCG ATATTTTTTT TTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACTAACCAGT GGCTGGAATG GGAAGCGACA GAGCTGCAGC CAGCTTTGTC TGCTGCCCTG 
TACTATTTAG TGGTCCAAGG CAAGAAGGGG GAAGATGTTC TTGGTTCAGT GCGGAGAGCC 
CTGACTCACA TTGACCACAG CTTGAGTCGT CAGAACTGTC CTTTCCTGGC TGGGGAGACA 
GAATCTCTAG CCGACATTGT TTTGTGGGGA GCCCTATACC CATTACTGCA AGATCCCGCC 
TACCTCCCTG AGGAGCTGAG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TACCCAGGAA 
CCATGTCAGC GAGCTGCAGA GACTGTACTG AAACAGCAAG GTGTCCTGGC TCTCCGGCCT 
TACCTCCAAA AGCAGCCCCA GCCCAGCCCC GCTGAGGGAA GGGCTGTCAC CAATGAGCCT 
GAGGAGGAGG AGCTGGCTAC CCTATCTGAG GAGGAGATTG CTATGGCTGT TACTGCTTGG 
GAGAAGGGCC TAGAAAGTTT GCCCCCGCTG CGGCCCCAGC AGAATCCAGT GTTGCCTGTG 
GCTGGAGAAA GGAATGTGCT CATCACCAGT GCCCTCCCTT ACGTCAACAA TGTCCCCCAC 
CTTGGGAACA TCATTGGTTG TGTGCTCAGT GCCGATGTCT TTGCCAGGTA CTCTCGCCTC 
CGCCAGTGGA ACACCCTCTA TCTGTGTGGG ACAGATGAGT ATGGTACAGC AACAGAGACC 
AAGGCTCTGG AGGAGGGACT AACCCCCCAG GAGATCTGCG ACAAGTACCA CATCATCCAT 
GCTGACATCT ACCGCTGGTT TAACATTTCG TTTGATATTT TTGGTCGCAC CACCACTCCA 
CAGCAGACCA AAATCACCCA GGACATTTTC CAGCAGTTGC TGAAACGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT GCGATGTGAG CACTGTGCTC GCTTCCTGGC TGACCGCTTC 
GTGGAGGGCG TGTGTCCCTT CTGTGGCTAT GAGGAGGCTC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATCAATGC TGTCGAGCTT AAGAAGCCTC AGTGTAAAGT CTGCCGATCA 
TGCCCTGTGG TGCAGTCGAG CCAGCACCTG TTTCTGGACC TGCCTAAGCT GGAGAAGCGA 
CTGGAGGAGT GGTTGGGGAG GACATTGCCT GGCAGTGACT GGACACCCAA TGCCCAGTTT 
ATCACCCGTT CTTGGCTTCG GGATGGCCTC AAGCCACGCT GCATAACCCG AGACCTCAAA 
TGGGGAACCC CTGTACCCTT AGAAGGTTTT GAAGACAAGG TATTCTATGT CTGGTTTGAT 
GCCACTATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAGTGGGA GAGATGGTGG 
AAGAACCCAG AGCAAGTGGA CCTGTATCAG TTCATGGCCA AAGACAATGT TCCTTTCCAT 
AGCTTAGTCT TTCCTTGCTC AGCCCTAGGA GCTGAGGATA ACTATACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTACCT GAACTATGAG GATGGGAAAT TCTCTAAGAG CCGCGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACG GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA TTCGGCCTGA GGGCCAGGAC AGTGCTTTCT CCTGGACGGA CCTGCTGCTG 
AAGAATAATT CTGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCTAAGT TCTTTGGGGG CTATGTGCCT GAGATGGTGC TCACCCCTGA TGATCAGCGC 
CTGCTGGCCC ATGTCACCCT GGAGCTCCAG CACTATCACC AGCTACTTGA GAAGGTTCGG 
ATCCGGGATG CCTTGCGCAG TATCCTCACC ATATCTCGAC ATGGCAACCA ATATATTCAG 
GTGAATGAGC CCTGGAAGCG GATTAAAGGC AGTGAGGCTG ACAGGCAACG GGCAGGAACA 
GTGACTGGCT TGGCAGTGAA TATAGCTGCC TTGCTCTCTG TCATGCTTCA GCCTTACATG 
CCCACGGTTA GTGCCACAAT CCAGGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCTGACAA ACTTCCTGTG TACCTTACCA GCAGGACACC AGATTGGCAC AGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTTTAA GGCAGCGCTT TGGAGGGGGC 
CAGGCAAAAA CGTCCCCGAA GCCAGCAGTT GTAGAGACTG TTACAACAGC CAAGCCACAG 
CAGATACAAG CGCTGATGGA TGAAGTGACA AAACAAGGAA ACATTGTCCG AGAACTGAAA 
GCACAAAAGG CAGACAAGAA CGAGGTTGCT GCGGAGGTGG CGAAACTCTT GGATCTAAAG 
AAACAGTTGG CTGTAGCTGA GGGGAAACCC CCTGAAGCCC CTAAAGGCAA GAAGAAAAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MARS1 ( XM_006719398.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719461.1 Homo sapiens methionyl-tRNA synthetase (MARS), transcript variant X1, mRNA.

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CloneID	OHu46556
Clone ID Related Accession (Same CDS sequence)	XM_006719398.3 , XM_006719398.4
Accession Version	XM_006719398.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	methionine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719398.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTTA CTGCTTGGGA GAAGGGCCTA GAAAGTTTGC CCCCGCTGCG GCCCCAGCAG 
AATCCAGTGT TGCCTGTGGC TGGAGAAAGG AATGTGCTCA TCACCAGTGC CCTCCCTTAC 
GTCAACAATG TCCCCCACCT TGGGAACATC ATTGGTTGTG TGCTCAGTGC CGATGTCTTT 
GCCAGGTACT CTCGCCTCCG CCAGTGGAAC ACCCTCTATC TGTGTGGGAC AGATGAGTAT 
GGTACAGCAA CAGAGACCAA GGCTCTGGAG GAGGGACTAA CCCCCCAGGA GATCTGCGAC 
AAGTACCACA TCATCCATGC TGACATCTAC CGCTGGTTTA ACATTTCGTT TGATATTTTT 
GGTCGCACCA CCACTCCACA GCAGACCAAA ATCACCCAGG ACATTTTCCA GCAGTTGCTG 
AAACGAGGTT TTGTGCTGCA AGATACTGTG GAGCAACTGC GATGTGAGCA CTGTGCTCGC 
TTCCTGGCTG ACCGCTTCGT GGAGGGCGTG TGTCCCTTCT GTGGCTATGA GGAGGCTCGG 
GGTGACCAGT GTGACAAGTG TGGCAAGCTC ATCAATGCTG TCGAGCTTAA GAAGCCTCAG 
TGTAAAGTCT GCCGATCATG CCCTGTGGTG CAGTCGAGCC AGCACCTGTT TCTGGACCTG 
CCTAAGCTGG AGAAGCGACT GGAGGAGTGG TTGGGGAGGA CATTGCCTGG CAGTGACTGG 
ACACCCAATG CCCAGTTTAT CACCCGTTCT TGGCTTCGGG ATGGCCTCAA GCCACGCTGC 
ATAACCCGAG ACCTCAAATG GGGAACCCCT GTACCCTTAG AAGGTTTTGA AGACAAGGTA 
TTCTATGTCT GGTTTGATGC CACTATTGGC TATCTGTCCA TCACAGCCAA CTACACAGAC 
CAGTGGGAGA GATGGTGGAA GAACCCAGAG CAAGTGGACC TGTATCAGTT CATGGCCAAA 
GACAATGTTC CTTTCCATAG CTTAGTCTTT CCTTGCTCAG CCCTAGGAGC TGAGGATAAC 
TATACCTTGG TCAGCCACCT CATTGCTACA GAGTACCTGA ACTATGAGGA TGGGAAATTC 
TCTAAGAGCC GCGGTGTGGG AGTGTTTGGG GACATGGCCC AGGACACGGG GATCCCTGCT 
GACATCTGGC GCTTCTATCT GCTGTACATT CGGCCTGAGG GCCAGGACAG TGCTTTCTCC 
TGGACGGACC TGCTGCTGAA GAATAATTCT GAGCTGCTTA ACAACCTGGG CAACTTCATC 
AACAGAGCTG GGATGTTTGT GTCTAAGTTC TTTGGGGGCT ATGTGCCTGA GATGGTGCTC 
ACCCCTGATG ATCAGCGCCT GCTGGCCCAT GTCACCCTGG AGCTCCAGCA CTATCACCAG 
CTACTTGAGA AGGTTCGGAT CCGGGATGCC TTGCGCAGTA TCCTCACCAT ATCTCGACAT 
GGCAACCAAT ATATTCAGGT GAATGAGCCC TGGAAGCGGA TTAAAGGCAG TGAGGCTGAC 
AGGCAACGGG CAGGAACAGT GACTGGCTTG GCAGTGAATA TAGCTGCCTT GCTCTCTGTC 
ATGCTTCAGC CTTACATGCC CACGGTTAGT GCCACAATCC AGGCCCAGCT GCAGCTCCCA 
CCTCCAGCCT GCAGTATCCT GCTGACAAAC TTCCTGTGTA CCTTACCAGC AGGACACCAG 
ATTGGCACAG TCAGTCCCTT GTTCCAAAAA TTGGAAAATG ACCAGATTGA AAGTTTAAGG 
CAGCGCTTTG GAGGGGGCCA GGCAAAAACG TCCCCGAAGC CAGCAGTTGT AGAGACTGTT 
ACAACAGCCA AGCCACAGCA GATACAAGCG CTGATGGATG AAGTGACAAA ACAAGGAAAC 
ATTGTCCGAG AACTGAAAGC ACAAAAGGCA GACAAGAACG AGGTTGCTGC GGAGGTGGCG 
AAACTCTTGG ATCTAAAGAA ACAGTTGGCT GTAGCTGAGG GGAAACCCCC TGAAGCCCCT 
AAAGGCAAGA AGAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719461.1
CDS	676..2676
Translation	MAVTAWEKGLESLPPLRPQQNPVLPVAGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKALEEGLTPQEICDKYHIIHADIYRWFNISFDIFGRTTTPQQTKITQDIFQQLLKRGFVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAVELKKPQCKVCRSCPVVQSSQHLFLDLPKLEKRLEEWLGRTLPGSDWTPNAQFITRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWERWWKNPEQVDLYQFMAKDNVPFHSLVFPCSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLLKNNSELLNNLGNFINRAGMFVSKFFGGYVPEMVLTPDDQRLLAHVTLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGSEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILLTNFLCTLPAGHQIGTVSPLFQKLENDQIESLRQRFGGGQAKTSPKPAVVETVTTAKPQQIQALMDEVTKQGNIVRELKAQKADKNEVAAEVAKLLDLKKQLAVAEGKPPEAPKGKKKK

Target ORF information:

RefSeq Version	XM_006719398.3
Organism	Homo sapiens(human)
Definition	Homo sapiens methionyl-tRNA synthetase (MARS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719398.3

ORF Insert Sequence:

ATGGCTGTTA CTGCTTGGGA GAAGGGCCTA GAAAGTTTGC CCCCGCTGCG GCCCCAGCAG 
AATCCAGTGT TGCCTGTGGC TGGAGAAAGG AATGTGCTCA TCACCAGTGC CCTCCCTTAC 
GTCAACAATG TCCCCCACCT TGGGAACATC ATTGGTTGTG TGCTCAGTGC CGATGTCTTT 
GCCAGGTACT CTCGCCTCCG CCAGTGGAAC ACCCTCTATC TGTGTGGGAC AGATGAGTAT 
GGTACAGCAA CAGAGACCAA GGCTCTGGAG GAGGGACTAA CCCCCCAGGA GATCTGCGAC 
AAGTACCACA TCATCCATGC TGACATCTAC CGCTGGTTTA ACATTTCGTT TGATATTTTT 
GGTCGCACCA CCACTCCACA GCAGACCAAA ATCACCCAGG ACATTTTCCA GCAGTTGCTG 
AAACGAGGTT TTGTGCTGCA AGATACTGTG GAGCAACTGC GATGTGAGCA CTGTGCTCGC 
TTCCTGGCTG ACCGCTTCGT GGAGGGCGTG TGTCCCTTCT GTGGCTATGA GGAGGCTCGG 
GGTGACCAGT GTGACAAGTG TGGCAAGCTC ATCAATGCTG TCGAGCTTAA GAAGCCTCAG 
TGTAAAGTCT GCCGATCATG CCCTGTGGTG CAGTCGAGCC AGCACCTGTT TCTGGACCTG 
CCTAAGCTGG AGAAGCGACT GGAGGAGTGG TTGGGGAGGA CATTGCCTGG CAGTGACTGG 
ACACCCAATG CCCAGTTTAT CACCCGTTCT TGGCTTCGGG ATGGCCTCAA GCCACGCTGC 
ATAACCCGAG ACCTCAAATG GGGAACCCCT GTACCCTTAG AAGGTTTTGA AGACAAGGTA 
TTCTATGTCT GGTTTGATGC CACTATTGGC TATCTGTCCA TCACAGCCAA CTACACAGAC 
CAGTGGGAGA GATGGTGGAA GAACCCAGAG CAAGTGGACC TGTATCAGTT CATGGCCAAA 
GACAATGTTC CTTTCCATAG CTTAGTCTTT CCTTGCTCAG CCCTAGGAGC TGAGGATAAC 
TATACCTTGG TCAGCCACCT CATTGCTACA GAGTACCTGA ACTATGAGGA TGGGAAATTC 
TCTAAGAGCC GCGGTGTGGG AGTGTTTGGG GACATGGCCC AGGACACGGG GATCCCTGCT 
GACATCTGGC GCTTCTATCT GCTGTACATT CGGCCTGAGG GCCAGGACAG TGCTTTCTCC 
TGGACGGACC TGCTGCTGAA GAATAATTCT GAGCTGCTTA ACAACCTGGG CAACTTCATC 
AACAGAGCTG GGATGTTTGT GTCTAAGTTC TTTGGGGGCT ATGTGCCTGA GATGGTGCTC 
ACCCCTGATG ATCAGCGCCT GCTGGCCCAT GTCACCCTGG AGCTCCAGCA CTATCACCAG 
CTACTTGAGA AGGTTCGGAT CCGGGATGCC TTGCGCAGTA TCCTCACCAT ATCTCGACAT 
GGCAACCAAT ATATTCAGGT GAATGAGCCC TGGAAGCGGA TTAAAGGCAG TGAGGCTGAC 
AGGCAACGGG CAGGAACAGT GACTGGCTTG GCAGTGAATA TAGCTGCCTT GCTCTCTGTC 
ATGCTTCAGC CTTACATGCC CACGGTTAGT GCCACAATCC AGGCCCAGCT GCAGCTCCCA 
CCTCCAGCCT GCAGTATCCT GCTGACAAAC TTCCTGTGTA CCTTACCAGC AGGACACCAG 
ATTGGCACAG TCAGTCCCTT GTTCCAAAAA TTGGAAAATG ACCAGATTGA AAGTTTAAGG 
CAGCGCTTTG GAGGGGGCCA GGCAAAAACG TCCCCGAAGC CAGCAGTTGT AGAGACTGTT 
ACAACAGCCA AGCCACAGCA GATACAAGCG CTGATGGATG AAGTGACAAA ACAAGGAAAC 
ATTGTCCGAG AACTGAAAGC ACAAAAGGCA GACAAGAACG AGGTTGCTGC GGAGGTGGCG 
AAACTCTTGG ATCTAAAGAA ACAGTTGGCT GTAGCTGAGG GGAAACCCCC TGAAGCCCCT 
AAAGGCAAGA AGAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MARS1 ( XM_006719398.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719461.1 Homo sapiens methionyl-tRNA synthetase 1 (MARS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu46556
Clone ID Related Accession (Same CDS sequence)	XM_006719398.3 , XM_006719398.4
Accession Version	XM_006719398.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	methionine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006719398.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTTA CTGCTTGGGA GAAGGGCCTA GAAAGTTTGC CCCCGCTGCG GCCCCAGCAG 
AATCCAGTGT TGCCTGTGGC TGGAGAAAGG AATGTGCTCA TCACCAGTGC CCTCCCTTAC 
GTCAACAATG TCCCCCACCT TGGGAACATC ATTGGTTGTG TGCTCAGTGC CGATGTCTTT 
GCCAGGTACT CTCGCCTCCG CCAGTGGAAC ACCCTCTATC TGTGTGGGAC AGATGAGTAT 
GGTACAGCAA CAGAGACCAA GGCTCTGGAG GAGGGACTAA CCCCCCAGGA GATCTGCGAC 
AAGTACCACA TCATCCATGC TGACATCTAC CGCTGGTTTA ACATTTCGTT TGATATTTTT 
GGTCGCACCA CCACTCCACA GCAGACCAAA ATCACCCAGG ACATTTTCCA GCAGTTGCTG 
AAACGAGGTT TTGTGCTGCA AGATACTGTG GAGCAACTGC GATGTGAGCA CTGTGCTCGC 
TTCCTGGCTG ACCGCTTCGT GGAGGGCGTG TGTCCCTTCT GTGGCTATGA GGAGGCTCGG 
GGTGACCAGT GTGACAAGTG TGGCAAGCTC ATCAATGCTG TCGAGCTTAA GAAGCCTCAG 
TGTAAAGTCT GCCGATCATG CCCTGTGGTG CAGTCGAGCC AGCACCTGTT TCTGGACCTG 
CCTAAGCTGG AGAAGCGACT GGAGGAGTGG TTGGGGAGGA CATTGCCTGG CAGTGACTGG 
ACACCCAATG CCCAGTTTAT CACCCGTTCT TGGCTTCGGG ATGGCCTCAA GCCACGCTGC 
ATAACCCGAG ACCTCAAATG GGGAACCCCT GTACCCTTAG AAGGTTTTGA AGACAAGGTA 
TTCTATGTCT GGTTTGATGC CACTATTGGC TATCTGTCCA TCACAGCCAA CTACACAGAC 
CAGTGGGAGA GATGGTGGAA GAACCCAGAG CAAGTGGACC TGTATCAGTT CATGGCCAAA 
GACAATGTTC CTTTCCATAG CTTAGTCTTT CCTTGCTCAG CCCTAGGAGC TGAGGATAAC 
TATACCTTGG TCAGCCACCT CATTGCTACA GAGTACCTGA ACTATGAGGA TGGGAAATTC 
TCTAAGAGCC GCGGTGTGGG AGTGTTTGGG GACATGGCCC AGGACACGGG GATCCCTGCT 
GACATCTGGC GCTTCTATCT GCTGTACATT CGGCCTGAGG GCCAGGACAG TGCTTTCTCC 
TGGACGGACC TGCTGCTGAA GAATAATTCT GAGCTGCTTA ACAACCTGGG CAACTTCATC 
AACAGAGCTG GGATGTTTGT GTCTAAGTTC TTTGGGGGCT ATGTGCCTGA GATGGTGCTC 
ACCCCTGATG ATCAGCGCCT GCTGGCCCAT GTCACCCTGG AGCTCCAGCA CTATCACCAG 
CTACTTGAGA AGGTTCGGAT CCGGGATGCC TTGCGCAGTA TCCTCACCAT ATCTCGACAT 
GGCAACCAAT ATATTCAGGT GAATGAGCCC TGGAAGCGGA TTAAAGGCAG TGAGGCTGAC 
AGGCAACGGG CAGGAACAGT GACTGGCTTG GCAGTGAATA TAGCTGCCTT GCTCTCTGTC 
ATGCTTCAGC CTTACATGCC CACGGTTAGT GCCACAATCC AGGCCCAGCT GCAGCTCCCA 
CCTCCAGCCT GCAGTATCCT GCTGACAAAC TTCCTGTGTA CCTTACCAGC AGGACACCAG 
ATTGGCACAG TCAGTCCCTT GTTCCAAAAA TTGGAAAATG ACCAGATTGA AAGTTTAAGG 
CAGCGCTTTG GAGGGGGCCA GGCAAAAACG TCCCCGAAGC CAGCAGTTGT AGAGACTGTT 
ACAACAGCCA AGCCACAGCA GATACAAGCG CTGATGGATG AAGTGACAAA ACAAGGAAAC 
ATTGTCCGAG AACTGAAAGC ACAAAAGGCA GACAAGAACG AGGTTGCTGC GGAGGTGGCG 
AAACTCTTGG ATCTAAAGAA ACAGTTGGCT GTAGCTGAGG GGAAACCCCC TGAAGCCCCT 
AAAGGCAAGA AGAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719461.1
CDS	650..2650
Translation	MAVTAWEKGLESLPPLRPQQNPVLPVAGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKALEEGLTPQEICDKYHIIHADIYRWFNISFDIFGRTTTPQQTKITQDIFQQLLKRGFVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAVELKKPQCKVCRSCPVVQSSQHLFLDLPKLEKRLEEWLGRTLPGSDWTPNAQFITRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWERWWKNPEQVDLYQFMAKDNVPFHSLVFPCSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLLKNNSELLNNLGNFINRAGMFVSKFFGGYVPEMVLTPDDQRLLAHVTLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGSEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILLTNFLCTLPAGHQIGTVSPLFQKLENDQIESLRQRFGGGQAKTSPKPAVVETVTTAKPQQIQALMDEVTKQGNIVRELKAQKADKNEVAAEVAKLLDLKKQLAVAEGKPPEAPKGKKKK

Target ORF information:

RefSeq Version	XM_006719398.4
Organism	Homo sapiens(human)
Definition	Homo sapiens methionyl-tRNA synthetase 1 (MARS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719398.4

ORF Insert Sequence:

ATGGCTGTTA CTGCTTGGGA GAAGGGCCTA GAAAGTTTGC CCCCGCTGCG GCCCCAGCAG 
AATCCAGTGT TGCCTGTGGC TGGAGAAAGG AATGTGCTCA TCACCAGTGC CCTCCCTTAC 
GTCAACAATG TCCCCCACCT TGGGAACATC ATTGGTTGTG TGCTCAGTGC CGATGTCTTT 
GCCAGGTACT CTCGCCTCCG CCAGTGGAAC ACCCTCTATC TGTGTGGGAC AGATGAGTAT 
GGTACAGCAA CAGAGACCAA GGCTCTGGAG GAGGGACTAA CCCCCCAGGA GATCTGCGAC 
AAGTACCACA TCATCCATGC TGACATCTAC CGCTGGTTTA ACATTTCGTT TGATATTTTT 
GGTCGCACCA CCACTCCACA GCAGACCAAA ATCACCCAGG ACATTTTCCA GCAGTTGCTG 
AAACGAGGTT TTGTGCTGCA AGATACTGTG GAGCAACTGC GATGTGAGCA CTGTGCTCGC 
TTCCTGGCTG ACCGCTTCGT GGAGGGCGTG TGTCCCTTCT GTGGCTATGA GGAGGCTCGG 
GGTGACCAGT GTGACAAGTG TGGCAAGCTC ATCAATGCTG TCGAGCTTAA GAAGCCTCAG 
TGTAAAGTCT GCCGATCATG CCCTGTGGTG CAGTCGAGCC AGCACCTGTT TCTGGACCTG 
CCTAAGCTGG AGAAGCGACT GGAGGAGTGG TTGGGGAGGA CATTGCCTGG CAGTGACTGG 
ACACCCAATG CCCAGTTTAT CACCCGTTCT TGGCTTCGGG ATGGCCTCAA GCCACGCTGC 
ATAACCCGAG ACCTCAAATG GGGAACCCCT GTACCCTTAG AAGGTTTTGA AGACAAGGTA 
TTCTATGTCT GGTTTGATGC CACTATTGGC TATCTGTCCA TCACAGCCAA CTACACAGAC 
CAGTGGGAGA GATGGTGGAA GAACCCAGAG CAAGTGGACC TGTATCAGTT CATGGCCAAA 
GACAATGTTC CTTTCCATAG CTTAGTCTTT CCTTGCTCAG CCCTAGGAGC TGAGGATAAC 
TATACCTTGG TCAGCCACCT CATTGCTACA GAGTACCTGA ACTATGAGGA TGGGAAATTC 
TCTAAGAGCC GCGGTGTGGG AGTGTTTGGG GACATGGCCC AGGACACGGG GATCCCTGCT 
GACATCTGGC GCTTCTATCT GCTGTACATT CGGCCTGAGG GCCAGGACAG TGCTTTCTCC 
TGGACGGACC TGCTGCTGAA GAATAATTCT GAGCTGCTTA ACAACCTGGG CAACTTCATC 
AACAGAGCTG GGATGTTTGT GTCTAAGTTC TTTGGGGGCT ATGTGCCTGA GATGGTGCTC 
ACCCCTGATG ATCAGCGCCT GCTGGCCCAT GTCACCCTGG AGCTCCAGCA CTATCACCAG 
CTACTTGAGA AGGTTCGGAT CCGGGATGCC TTGCGCAGTA TCCTCACCAT ATCTCGACAT 
GGCAACCAAT ATATTCAGGT GAATGAGCCC TGGAAGCGGA TTAAAGGCAG TGAGGCTGAC 
AGGCAACGGG CAGGAACAGT GACTGGCTTG GCAGTGAATA TAGCTGCCTT GCTCTCTGTC 
ATGCTTCAGC CTTACATGCC CACGGTTAGT GCCACAATCC AGGCCCAGCT GCAGCTCCCA 
CCTCCAGCCT GCAGTATCCT GCTGACAAAC TTCCTGTGTA CCTTACCAGC AGGACACCAG 
ATTGGCACAG TCAGTCCCTT GTTCCAAAAA TTGGAAAATG ACCAGATTGA AAGTTTAAGG 
CAGCGCTTTG GAGGGGGCCA GGCAAAAACG TCCCCGAAGC CAGCAGTTGT AGAGACTGTT 
ACAACAGCCA AGCCACAGCA GATACAAGCG CTGATGGATG AAGTGACAAA ACAAGGAAAC 
ATTGTCCGAG AACTGAAAGC ACAAAAGGCA GACAAGAACG AGGTTGCTGC GGAGGTGGCG 
AAACTCTTGG ATCTAAAGAA ACAGTTGGCT GTAGCTGAGG GGAAACCCCC TGAAGCCCCT 
AAAGGCAAGA AGAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly of Multi-tRNA Synthetase Complex via Heterotetrameric Glutathione Transferase-homology Domains.
The Journal of biological chemistry290(49)29313-28(2015 Dec)
Cho HY,Maeng SJ,Cho HJ,Choi YS,Chung JM,Lee S,Kim HK,Kim JH,Eom CY,Kim YG,Guo M,Jung HS,Kang BS,Kim S

Biallelic Mutations of Methionyl-tRNA Synthetase Cause a Specific Type of Pulmonary Alveolar Proteinosis Prevalent on R?union Island.
American journal of human genetics96(5)826-31(2015 May)
Hadchouel A,Wieland T,Griese M,Baruffini E,Lorenz-Depiereux B,Enaud L,Graf E,Dubus JC,Halioui-Louhaichi S,Coulomb A,Delacourt C,Eckstein G,Zarbock R,Schwarzmayr T,Cartault F,Meitinger T,Lodi T,de Blic J,Strom TM

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MARS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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