MARS cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MARS
Entrez Gene ID	512531
Full Name	methionyl-tRNA synthetase
General protein information	Preferred Names methionyl-tRNA synthetase Names methionine--tRNA ligase, cytoplasmic metRS methionine-tRNA synthetase methionyl-tRNA synthetase, cytoplasmic
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010805159.2	XP_010803461.1	methionine--tRNA ligase, cytoplasmic isoform X1
XM_015471086.2	XP_015326572.1	methionine--tRNA ligase, cytoplasmic isoform X2
NM_001038091.2	NP_001033180.1	methionine--tRNA ligase, cytoplasmic
XM_015471086.1	XP_015326572.1	methionine--tRNA ligase, cytoplasmic isoform X2
XM_024991643.1	XP_024847411.1	methionine--tRNA ligase, cytoplasmic isoform X1
XM_010805159.3	XP_010803461.1	methionine--tRNA ligase, cytoplasmic isoform X1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0A09647g	XP_451421.1
Bos taurus (cattle)	MARS	NP_001033180.1
Mus musculus (house mouse)	Mars	NP_001165053.1
Rattus norvegicus (Norway rat)	Mars	NP_001121131.1
Schizosaccharomyces pombe (fission yeast)	rar1	NP_595586.1
Homo sapiens (human)	MARS	NP_004981.2
Pan troglodytes (chimpanzee)	MARS	XP_509164.2
	MARS	XP_001116063.1
Canis lupus familiaris (dog)	MARS	XP_531646.2
Gallus gallus (chicken)	MARS	NP_001244301.1
Danio rerio (zebrafish)	mars	NP_956370.1
Eremothecium gossypii	AGOS_AGR332C	NP_986998.1
Xenopus tropicalis (tropical clawed frog)	mars	NP_001017000.2
Drosophila melanogaster (fruit fly)	CG15100	NP_611382.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007891	XP_317601.4
Neurospora crassa	NCU07451	XP_964638.1
Saccharomyces cerevisiae (baker's yeast)	MES1	NP_011780.3
Magnaporthe oryzae (rice blast fungus)	MGG_01046	XP_003717896.1
Oryza sativa (rice)	Os10g0400100	NP_001064541.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa00507	NM_001038091.2 Latest version!	Bos taurus methionyl-tRNA synthetase 1 (MARS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa19420	XM_010805159.3 Latest version!	Bos taurus methionyl-tRNA synthetase (MARS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa19420	XM_024991643.1 Latest version!	Bos taurus methionyl-tRNA synthetase (MARS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa90749	XM_015471086.2 Latest version!	Bos taurus methionyl-tRNA synthetase (MARS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OBa90749	XM_015471086.1	Bos taurus methionyl-tRNA synthetase (MARS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa19420	XM_010805159.2	Bos taurus methionyl-tRNA synthetase (MARS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MARS ( NM_001038091.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001033180.1 Bos taurus methionyl-tRNA synthetase 1 (MARS1), mRNA.

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CloneID	OBa00507
Clone ID Related Accession (Same CDS sequence)	NM_001038091.2
Accession Version	NM_001038091.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	methionine--tRNA ligase, cytoplasmic
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC111362.1. On Aug 31, 2012 this sequence version replaced NM_001038091.1. ##Evidence-Data-START## Transcript exon combination :: BC111362.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAGGTTGT TCGTGAGTGA GGGCGCTCCG GGGAGTCTGC CTGTGCTGGC CGCGGCCGGG 
AGGGCCCAGG GCAGAGCGGA GCTGCTCATC AGCACTGTAG GCCCAGAAGA GTGTGTGGTC 
CCATTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCCACCAGTG CAATCTGTCG ATACTTCTTT CTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACCAACCAGT GGCTGGAATG GGAAGCAACC GAGCTGCAGC CAGCTTTGTC CGCTGCCCTG 
TACTATTTAG TGGTCCAAGG GAAGAAGGGA GAAGATGTTC TTGGCCCAGT TCGGAGAGCC 
CTGACGCATA TTGACCACAG CTTGAGTCGT CGGAGCTGTC CGTTCCTGGC TGGGGAGACA 
GAATCTCTAG CTGATATTGT TTTGTGGGGA GCGCTCTACC CGTTACTCCA AGACCCATCC 
TATCTCCCTG AGGAGCTGGG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TTCTCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAGCAGCAGG GTGTCCTGGC CCTCCGGCCC 
TACCTCCAGA AGCAGCCCCA GCCCAGCTCC CTTGAGGGCA GGCTTGTCAG CAATGAGCCT 
GAGGAGGAGG AACTGGCTAC TCTGTCTGAG GAGGAGATTG CCGTGGCTGT AGCTGCTTGG 
GAGAAGGGCC TGGAAAGCTT GCCCCCTCTT CGGCCGCAGC AGAATCCAGT GTTGCCCGTG 
GCTGGGGAGA GAAATGTGCT CATCACCAGC GCCCTCCCTT ACGTCAACAA CGTCCCCCAC 
CTTGGAAACA TCATCGGCTG TGTGCTCAGT GCCGACGTCT TTGCAAGGTA CTCCCGGCTC 
CGTCAGTGGA ACACCCTCTA CCTGTGTGGG ACAGATGAGT ATGGTACAGC GACAGAGACC 
AAGGCTATGG AGGAGGGACT AACCCCACAG GAAATCTGTG ACAAGTACCA CGTAATCCAC 
GCTGACATCT ACCGCTGGTT TAATATTTCG TTTGACTTTT TTGGTCGGAC TACCACTCCA 
CAGCAGACCA AAATTACCCA GGACATCTTC CAGCGGCTGT TGGCCCGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT TCGGTGTGAG CACTGTGCCC GCTTCCTGGC TGACCGCTTT 
GTGGAGGGTG TGTGTCCCTT CTGTGGATAC GAGGAAGCCC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATTAATGC CATTGAGCTC AAGAAGCCTC AGTGTAAAGT CTGCCGGTCG 
TGCCCTGTGG TGAAATCCTC TCAGCACCTG TTTCTGGACC TGCCTAAGCT GGCAGCGCGG 
GTGGAGGAGT GGTTGGAGAA GACATTGCCT GGCAGTGACT GGACTGCCAA CGCCCGGTTC 
ATCATTCGTT CTTGGCTTCG AGATGGCCTC AAGCCCCGCT GTATAACCCG AGACCTCAAG 
TGGGGAACCC CTGTGCCCTT AGAAGGTTTT GAGGACAAGG TGTTCTATGT CTGGTTTGAT 
GCCACCATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAATGGGA GAAGTGGTGG 
AAGAACCCAG AACAAGTGAA CCTGTATCAG TTCATGGCCA AGGACAATGT TCCCTTCCAT 
GGCATAGTCT TTCCTTCTTC AGCCCTAGGA GCTGAGGACA ACTACACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTATCT GAATTACGAG GATGGGAAAT TCTCTAAGAG CCGGGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACA GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA ATCGGCCTGA GGGGCAGGAC AGTGCCTTCT CCTGGACAGA CATGTTGTTC 
AAGAATAATT CCGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCCAAGT TTTTTGGGGG TTTTGTGCCT GAGATGGTGC TCACCTCTGA TGATCAGCGC 
CTGCTGACCC ATATCACCCT GGAGCTCCAG CACTACCACC AGCTGCTAGA GAAGGTTCGG 
ATCCGGGATG CCTTACGTAG CATCCTCACC ATCTCTCGCC ATGGCAACCA GTACATTCAG 
GTGAACGAGC CCTGGAAGCG GATTAAAGGT GGCGAAGCTG ACAGGCAGCG GGCAGGGACA 
GTGACAGGCT TGGCCGTGAA TATAGCTGCC TTGCTGTCCG TCATGCTCCA GCCTTACATG 
CCTACAGTTA GTGCCACCAT CCAAGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCCCACAA ACTTCCTATG CACCTTACCA GCAGGACACC AGATTGGCAC GGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTCTGA AGCAGCGCTT CAGTGGGGGC 
CAGGCAAAAG CATCCCCCAA GACAGCAGCT GGACTGACAA CAGCTGGACC GCAGCAGATC 
CAAGCACTGA CAGAGGAAGT GACCAAGCAG GGCAATATTG TCCGAGAATT GAAGGCACAG 
AAGGCAGACA AGAACCAGAT TGCTGCAGAG GTGGCTAAAC TCTTGGATCT GAAGAAACAG 
TTGGCTCTAG CTGAGGGGAA ACCCCTGGAA ACCTCTAAAG GCAAGAAGAA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033180.1
CDS	54..2750
Translation	MRLFVSEGAPGSLPVLAAAGRAQGRAELLISTVGPEECVVPFLTRPKVPVLQLDSGNYLFSTSAICRYFFLLSGWEQDDLTNQWLEWEATELQPALSAALYYLVVQGKKGEDVLGPVRRALTHIDHSLSRRSCPFLAGETESLADIVLWGALYPLLQDPSYLPEELGALHSWFQTLSSQEPCQRAAETVLKQQGVLALRPYLQKQPQPSSLEGRLVSNEPEEEELATLSEEEIAVAVAAWEKGLESLPPLRPQQNPVLPVAGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKAMEEGLTPQEICDKYHVIHADIYRWFNISFDFFGRTTTPQQTKITQDIFQRLLARGFVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAIELKKPQCKVCRSCPVVKSSQHLFLDLPKLAARVEEWLEKTLPGSDWTANARFIIRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWEKWWKNPEQVNLYQFMAKDNVPFHGIVFPSSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYNRPEGQDSAFSWTDMLFKNNSELLNNLGNFINRAGMFVSKFFGGFVPEMVLTSDDQRLLTHITLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGGEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILPTNFLCTLPAGHQIGTVSPLFQKLENDQIESLKQRFSGGQAKASPKTAAGLTTAGPQQIQALTEEVTKQGNIVRELKAQKADKNQIAAEVAKLLDLKKQLALAEGKPLETSKGKKKK

Target ORF information:

RefSeq Version	NM_001038091.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methionyl-tRNA synthetase 1 (MARS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038091.2

ORF Insert Sequence:

ATGAGGTTGT TCGTGAGTGA GGGCGCTCCG GGGAGTCTGC CTGTGCTGGC CGCGGCCGGG 
AGGGCCCAGG GCAGAGCGGA GCTGCTCATC AGCACTGTAG GCCCAGAAGA GTGTGTGGTC 
CCATTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCCACCAGTG CAATCTGTCG ATACTTCTTT CTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACCAACCAGT GGCTGGAATG GGAAGCAACC GAGCTGCAGC CAGCTTTGTC CGCTGCCCTG 
TACTATTTAG TGGTCCAAGG GAAGAAGGGA GAAGATGTTC TTGGCCCAGT TCGGAGAGCC 
CTGACGCATA TTGACCACAG CTTGAGTCGT CGGAGCTGTC CGTTCCTGGC TGGGGAGACA 
GAATCTCTAG CTGATATTGT TTTGTGGGGA GCGCTCTACC CGTTACTCCA AGACCCATCC 
TATCTCCCTG AGGAGCTGGG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TTCTCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAGCAGCAGG GTGTCCTGGC CCTCCGGCCC 
TACCTCCAGA AGCAGCCCCA GCCCAGCTCC CTTGAGGGCA GGCTTGTCAG CAATGAGCCT 
GAGGAGGAGG AACTGGCTAC TCTGTCTGAG GAGGAGATTG CCGTGGCTGT AGCTGCTTGG 
GAGAAGGGCC TGGAAAGCTT GCCCCCTCTT CGGCCGCAGC AGAATCCAGT GTTGCCCGTG 
GCTGGGGAGA GAAATGTGCT CATCACCAGC GCCCTCCCTT ACGTCAACAA CGTCCCCCAC 
CTTGGAAACA TCATCGGCTG TGTGCTCAGT GCCGACGTCT TTGCAAGGTA CTCCCGGCTC 
CGTCAGTGGA ACACCCTCTA CCTGTGTGGG ACAGATGAGT ATGGTACAGC GACAGAGACC 
AAGGCTATGG AGGAGGGACT AACCCCACAG GAAATCTGTG ACAAGTACCA CGTAATCCAC 
GCTGACATCT ACCGCTGGTT TAATATTTCG TTTGACTTTT TTGGTCGGAC TACCACTCCA 
CAGCAGACCA AAATTACCCA GGACATCTTC CAGCGGCTGT TGGCCCGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT TCGGTGTGAG CACTGTGCCC GCTTCCTGGC TGACCGCTTT 
GTGGAGGGTG TGTGTCCCTT CTGTGGATAC GAGGAAGCCC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATTAATGC CATTGAGCTC AAGAAGCCTC AGTGTAAAGT CTGCCGGTCG 
TGCCCTGTGG TGAAATCCTC TCAGCACCTG TTTCTGGACC TGCCTAAGCT GGCAGCGCGG 
GTGGAGGAGT GGTTGGAGAA GACATTGCCT GGCAGTGACT GGACTGCCAA CGCCCGGTTC 
ATCATTCGTT CTTGGCTTCG AGATGGCCTC AAGCCCCGCT GTATAACCCG AGACCTCAAG 
TGGGGAACCC CTGTGCCCTT AGAAGGTTTT GAGGACAAGG TGTTCTATGT CTGGTTTGAT 
GCCACCATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAATGGGA GAAGTGGTGG 
AAGAACCCAG AACAAGTGAA CCTGTATCAG TTCATGGCCA AGGACAATGT TCCCTTCCAT 
GGCATAGTCT TTCCTTCTTC AGCCCTAGGA GCTGAGGACA ACTACACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTATCT GAATTACGAG GATGGGAAAT TCTCTAAGAG CCGGGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACA GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA ATCGGCCTGA GGGGCAGGAC AGTGCCTTCT CCTGGACAGA CATGTTGTTC 
AAGAATAATT CCGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCCAAGT TTTTTGGGGG TTTTGTGCCT GAGATGGTGC TCACCTCTGA TGATCAGCGC 
CTGCTGACCC ATATCACCCT GGAGCTCCAG CACTACCACC AGCTGCTAGA GAAGGTTCGG 
ATCCGGGATG CCTTACGTAG CATCCTCACC ATCTCTCGCC ATGGCAACCA GTACATTCAG 
GTGAACGAGC CCTGGAAGCG GATTAAAGGT GGCGAAGCTG ACAGGCAGCG GGCAGGGACA 
GTGACAGGCT TGGCCGTGAA TATAGCTGCC TTGCTGTCCG TCATGCTCCA GCCTTACATG 
CCTACAGTTA GTGCCACCAT CCAAGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCCCACAA ACTTCCTATG CACCTTACCA GCAGGACACC AGATTGGCAC GGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTCTGA AGCAGCGCTT CAGTGGGGGC 
CAGGCAAAAG CATCCCCCAA GACAGCAGCT GGACTGACAA CAGCTGGACC GCAGCAGATC 
CAAGCACTGA CAGAGGAAGT GACCAAGCAG GGCAATATTG TCCGAGAATT GAAGGCACAG 
AAGGCAGACA AGAACCAGAT TGCTGCAGAG GTGGCTAAAC TCTTGGATCT GAAGAAACAG 
TTGGCTCTAG CTGAGGGGAA ACCCCTGGAA ACCTCTAAAG GCAAGAAGAA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MARS ( XM_010805159.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803461.1 Bos taurus methionyl-tRNA synthetase (MARS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa19420
Clone ID Related Accession (Same CDS sequence)	XM_010805159.2 , XM_024991643.1 , XM_010805159.3
Accession Version	XM_010805159.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methionine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805159.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGT TCGTGAGTGA GGGCGCTCCG GGGAGTCTGC CTGTGCTGGC CGCGGCCGGG 
AGGGCCCAGG GCAGAGCGGA GCTGCTCATC AGCACTGTAG GCCCAGAAGA GTGTGTGGTC 
CCATTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCCACCAGTG CAATCTGTCG ATACTTCTTT CTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACCAACCAGT GGCTGGAATG GGAAGCAACC GAGCTGCAGC CAGCTTTGTC CGCTGCCCTG 
TACTATTTAG TGGTCCAAGG GAAGAAGGGA GAAGATGTTC TTGGCCCAGT TCGGAGAGCC 
CTGACGCATA TTGACCACAG CTTGAGTCGT CGGAGCTGTC CGTTCCTGGC TGGGGAGACA 
GAATCTCTAG CTGATATTGT TTTGTGGGGA GCGCTCTACC CGTTACTCCA AGACCCATCC 
TATCTCCCTG AGGAGCTGGG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TTCTCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAGCAGCAGG GTGTCCTGGC CCTCCGGCCC 
TACCTCCAGA AGCAGCCCCA GCCCAGCTCC CTTGAGGGCA GGCTTGTCAG CAATGAGCCT 
GAGGAGGAGG AACTGGCTAC TCTGTCTGAG GAGGAGATTG CCGTGGCTGT AGCTGCTTGG 
GAGAAGGGCC TGGAAAGCTT GCCCCCTCTT CGGCCGCAGC AGAATCCAGT GTTGCCCGTG 
GCTGGGGAGA GAAATGTGCT CATCACCAGC GCCCTCCCTT ACGTCAACAA CGTCCCCCAC 
CTTGGAAACA TCATCGGCTG TGTGCTCAGT GCCGACGTCT TTGCAAGGTA CTCCCGGCTC 
CGTCAGTGGA ACACCCTCTA CCTGTGTGGG ACAGATGAGT ATGGTACAGC GACAGAGACC 
AAGGCTATGG AGGAGGGACT AACCCCACAG GAGATCTGTG ACAAGTACCA CGTTATCCAC 
GCTGACATCT ACCGCTGGTT TAATATTTCG TTTGACTTTT TTGGTCGGAC TACCACTCCA 
CAGCAGACCA AAATTACCCA GGACATCTTC CAGCGGCTGT TGGCCCGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT TCGGTGTGAG CACTGTGCCC GCTTCCTGGC TGACCGCTTT 
GTGGAGGGTG TATGTCCCTT CTGTGGCTAC GAGGAAGCCC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATCAATGC CATTGAGCTC AAGAAGCCTC AGTGTAAAGT CTGCCGGTCG 
TGCCCTGTGG TGAAATCCTC TCAGCACCTG TTTCTGGACC TGCCTAAGCT GGCAGCGCGG 
GTGGAGGAGT GGTTGGAGAA GACATTGCCT GGCAGTGACT GGACTGCCAA CGCCCGGTTC 
ATCATTCGTT CTTGGCTTCG AGATGGCCTC AAGCCCCGCT GTATAACCCG AGACCTCAAG 
TGGGGAACCC CTGTGCCCTT AGAAGGTTTT GAGGACAAGG TGTTCTATGT CTGGTTTGAT 
GCCACCATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAATGGGA GAAGTGGTGG 
AAGAACCCAG AACAAGTGAA CCTGTATCAG TTCATGGCCA AGGACAATGT TCCCTTCCAT 
GGCATAGTCT TTCCTTCTTC AGCCCTAGGA GCTGAGGACA ACTACACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTATCT GAATTACGAG GATGGGAAAT TCTCTAAGAG CCGGGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACA GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA TTCGGCCTGA GGGGCAGGAC AGTGCCTTCT CCTGGACAGA CATGTTGTTC 
AAGAATAATT CCGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCCAAGT TTTTTGGGGG TTTTGTGCCT GAGATGGTGC TCACCTCTGA TGATCAGCGC 
CTGCTGACCC ATATCACCCT GGAGCTCCAG CACTACCACC AGCTGCTAGA GAAGGTTCGG 
ATCCGGGATG CCTTACGTAG CATCCTCACC ATCTCTCGCC ATGGCAACCA GTACATTCAG 
GTGAACGAGC CCTGGAAGCG GATTAAAGGT GGCGAAGCTG ACAGGCAGCG GGCAGGGACA 
GTGACAGGCT TGGCCGTGAA TATAGCTGCC TTGCTGTCCG TCATGCTCCA GCCTTACATG 
CCTACAGTTA GTGCCACCAT CCAAGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCCCACAA ACTTCCTATG CACCTTACCA GCAGGACACC AGATTGGCAC GGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTCTGA AGCAGCGCTT CAGTGGGGGC 
CAGCTAGAAG AGTCCTTGGC ATTAAAGGCA AAAGCATCCC CCAAGACAGC AGCTGGACTG 
ACAACAGCTG GACCGCAGCA GATCCAAGCA CTGACAGAGG AAGTGACCAA GCAGGGCAAT 
ATTGTCCGAG AATTGAAGGC ACAGAAGGCA GACAAGAACC AGATTGCTGC AGAGGTGGCT 
AAACTCTTGG ATCTGAAGAA ACAGTTGGCT CTAGCTGAGG GGAAACCCCT GGAAACCTCT 
AAAGGCAAGA AGAAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803461.1
CDS	244..2964
Translation	MRLFVSEGAPGSLPVLAAAGRAQGRAELLISTVGPEECVVPFLTRPKVPVLQLDSGNYLFSTSAICRYFFLLSGWEQDDLTNQWLEWEATELQPALSAALYYLVVQGKKGEDVLGPVRRALTHIDHSLSRRSCPFLAGETESLADIVLWGALYPLLQDPSYLPEELGALHSWFQTLSSQEPCQRAAETVLKQQGVLALRPYLQKQPQPSSLEGRLVSNEPEEEELATLSEEEIAVAVAAWEKGLESLPPLRPQQNPVLPVAGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKAMEEGLTPQEICDKYHVIHADIYRWFNISFDFFGRTTTPQQTKITQDIFQRLLARGFVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAIELKKPQCKVCRSCPVVKSSQHLFLDLPKLAARVEEWLEKTLPGSDWTANARFIIRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWEKWWKNPEQVNLYQFMAKDNVPFHGIVFPSSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDMLFKNNSELLNNLGNFINRAGMFVSKFFGGFVPEMVLTSDDQRLLTHITLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGGEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILPTNFLCTLPAGHQIGTVSPLFQKLENDQIESLKQRFSGGQLEESLALKAKASPKTAAGLTTAGPQQIQALTEEVTKQGNIVRELKAQKADKNQIAAEVAKLLDLKKQLALAEGKPLETSKGKKKK

Target ORF information:

RefSeq Version	XM_010805159.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methionyl-tRNA synthetase (MARS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805159.2

ORF Insert Sequence:

ATGAGGTTGT TCGTGAGTGA GGGCGCTCCG GGGAGTCTGC CTGTGCTGGC CGCGGCCGGG 
AGGGCCCAGG GCAGAGCGGA GCTGCTCATC AGCACTGTAG GCCCAGAAGA GTGTGTGGTC 
CCATTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCCACCAGTG CAATCTGTCG ATACTTCTTT CTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACCAACCAGT GGCTGGAATG GGAAGCAACC GAGCTGCAGC CAGCTTTGTC CGCTGCCCTG 
TACTATTTAG TGGTCCAAGG GAAGAAGGGA GAAGATGTTC TTGGCCCAGT TCGGAGAGCC 
CTGACGCATA TTGACCACAG CTTGAGTCGT CGGAGCTGTC CGTTCCTGGC TGGGGAGACA 
GAATCTCTAG CTGATATTGT TTTGTGGGGA GCGCTCTACC CGTTACTCCA AGACCCATCC 
TATCTCCCTG AGGAGCTGGG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TTCTCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAGCAGCAGG GTGTCCTGGC CCTCCGGCCC 
TACCTCCAGA AGCAGCCCCA GCCCAGCTCC CTTGAGGGCA GGCTTGTCAG CAATGAGCCT 
GAGGAGGAGG AACTGGCTAC TCTGTCTGAG GAGGAGATTG CCGTGGCTGT AGCTGCTTGG 
GAGAAGGGCC TGGAAAGCTT GCCCCCTCTT CGGCCGCAGC AGAATCCAGT GTTGCCCGTG 
GCTGGGGAGA GAAATGTGCT CATCACCAGC GCCCTCCCTT ACGTCAACAA CGTCCCCCAC 
CTTGGAAACA TCATCGGCTG TGTGCTCAGT GCCGACGTCT TTGCAAGGTA CTCCCGGCTC 
CGTCAGTGGA ACACCCTCTA CCTGTGTGGG ACAGATGAGT ATGGTACAGC GACAGAGACC 
AAGGCTATGG AGGAGGGACT AACCCCACAG GAGATCTGTG ACAAGTACCA CGTTATCCAC 
GCTGACATCT ACCGCTGGTT TAATATTTCG TTTGACTTTT TTGGTCGGAC TACCACTCCA 
CAGCAGACCA AAATTACCCA GGACATCTTC CAGCGGCTGT TGGCCCGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT TCGGTGTGAG CACTGTGCCC GCTTCCTGGC TGACCGCTTT 
GTGGAGGGTG TATGTCCCTT CTGTGGCTAC GAGGAAGCCC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATCAATGC CATTGAGCTC AAGAAGCCTC AGTGTAAAGT CTGCCGGTCG 
TGCCCTGTGG TGAAATCCTC TCAGCACCTG TTTCTGGACC TGCCTAAGCT GGCAGCGCGG 
GTGGAGGAGT GGTTGGAGAA GACATTGCCT GGCAGTGACT GGACTGCCAA CGCCCGGTTC 
ATCATTCGTT CTTGGCTTCG AGATGGCCTC AAGCCCCGCT GTATAACCCG AGACCTCAAG 
TGGGGAACCC CTGTGCCCTT AGAAGGTTTT GAGGACAAGG TGTTCTATGT CTGGTTTGAT 
GCCACCATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAATGGGA GAAGTGGTGG 
AAGAACCCAG AACAAGTGAA CCTGTATCAG TTCATGGCCA AGGACAATGT TCCCTTCCAT 
GGCATAGTCT TTCCTTCTTC AGCCCTAGGA GCTGAGGACA ACTACACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTATCT GAATTACGAG GATGGGAAAT TCTCTAAGAG CCGGGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACA GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA TTCGGCCTGA GGGGCAGGAC AGTGCCTTCT CCTGGACAGA CATGTTGTTC 
AAGAATAATT CCGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCCAAGT TTTTTGGGGG TTTTGTGCCT GAGATGGTGC TCACCTCTGA TGATCAGCGC 
CTGCTGACCC ATATCACCCT GGAGCTCCAG CACTACCACC AGCTGCTAGA GAAGGTTCGG 
ATCCGGGATG CCTTACGTAG CATCCTCACC ATCTCTCGCC ATGGCAACCA GTACATTCAG 
GTGAACGAGC CCTGGAAGCG GATTAAAGGT GGCGAAGCTG ACAGGCAGCG GGCAGGGACA 
GTGACAGGCT TGGCCGTGAA TATAGCTGCC TTGCTGTCCG TCATGCTCCA GCCTTACATG 
CCTACAGTTA GTGCCACCAT CCAAGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCCCACAA ACTTCCTATG CACCTTACCA GCAGGACACC AGATTGGCAC GGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTCTGA AGCAGCGCTT CAGTGGGGGC 
CAGCTAGAAG AGTCCTTGGC ATTAAAGGCA AAAGCATCCC CCAAGACAGC AGCTGGACTG 
ACAACAGCTG GACCGCAGCA GATCCAAGCA CTGACAGAGG AAGTGACCAA GCAGGGCAAT 
ATTGTCCGAG AATTGAAGGC ACAGAAGGCA GACAAGAACC AGATTGCTGC AGAGGTGGCT 
AAACTCTTGG ATCTGAAGAA ACAGTTGGCT CTAGCTGAGG GGAAACCCCT GGAAACCTCT 
AAAGGCAAGA AGAAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MARS ( XM_024991643.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847411.1 Bos taurus methionyl-tRNA synthetase (MARS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa19420
Clone ID Related Accession (Same CDS sequence)	XM_010805159.2 , XM_024991643.1 , XM_010805159.3
Accession Version	XM_024991643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methionine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGT TCGTGAGTGA GGGCGCTCCG GGGAGTCTGC CTGTGCTGGC CGCGGCCGGG 
AGGGCCCAGG GCAGAGCGGA GCTGCTCATC AGCACTGTAG GCCCAGAAGA GTGTGTGGTC 
CCATTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCCACCAGTG CAATCTGTCG ATACTTCTTT CTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACCAACCAGT GGCTGGAATG GGAAGCAACC GAGCTGCAGC CAGCTTTGTC CGCTGCCCTG 
TACTATTTAG TGGTCCAAGG GAAGAAGGGA GAAGATGTTC TTGGCCCAGT TCGGAGAGCC 
CTGACGCATA TTGACCACAG CTTGAGTCGT CGGAGCTGTC CGTTCCTGGC TGGGGAGACA 
GAATCTCTAG CTGATATTGT TTTGTGGGGA GCGCTCTACC CGTTACTCCA AGACCCATCC 
TATCTCCCTG AGGAGCTGGG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TTCTCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAGCAGCAGG GTGTCCTGGC CCTCCGGCCC 
TACCTCCAGA AGCAGCCCCA GCCCAGCTCC CTTGAGGGCA GGCTTGTCAG CAATGAGCCT 
GAGGAGGAGG AACTGGCTAC TCTGTCTGAG GAGGAGATTG CCGTGGCTGT AGCTGCTTGG 
GAGAAGGGCC TGGAAAGCTT GCCCCCTCTT CGGCCGCAGC AGAATCCAGT GTTGCCCGTG 
GCTGGGGAGA GAAATGTGCT CATCACCAGC GCCCTCCCTT ACGTCAACAA CGTCCCCCAC 
CTTGGAAACA TCATCGGCTG TGTGCTCAGT GCCGACGTCT TTGCAAGGTA CTCCCGGCTC 
CGTCAGTGGA ACACCCTCTA CCTGTGTGGG ACAGATGAGT ATGGTACAGC GACAGAGACC 
AAGGCTATGG AGGAGGGACT AACCCCACAG GAGATCTGTG ACAAGTACCA CGTTATCCAC 
GCTGACATCT ACCGCTGGTT TAATATTTCG TTTGACTTTT TTGGTCGGAC TACCACTCCA 
CAGCAGACCA AAATTACCCA GGACATCTTC CAGCGGCTGT TGGCCCGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT TCGGTGTGAG CACTGTGCCC GCTTCCTGGC TGACCGCTTT 
GTGGAGGGTG TATGTCCCTT CTGTGGCTAC GAGGAAGCCC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATCAATGC CATTGAGCTC AAGAAGCCTC AGTGTAAAGT CTGCCGGTCG 
TGCCCTGTGG TGAAATCCTC TCAGCACCTG TTTCTGGACC TGCCTAAGCT GGCAGCGCGG 
GTGGAGGAGT GGTTGGAGAA GACATTGCCT GGCAGTGACT GGACTGCCAA CGCCCGGTTC 
ATCATTCGTT CTTGGCTTCG AGATGGCCTC AAGCCCCGCT GTATAACCCG AGACCTCAAG 
TGGGGAACCC CTGTGCCCTT AGAAGGTTTT GAGGACAAGG TGTTCTATGT CTGGTTTGAT 
GCCACCATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAATGGGA GAAGTGGTGG 
AAGAACCCAG AACAAGTGAA CCTGTATCAG TTCATGGCCA AGGACAATGT TCCCTTCCAT 
GGCATAGTCT TTCCTTCTTC AGCCCTAGGA GCTGAGGACA ACTACACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTATCT GAATTACGAG GATGGGAAAT TCTCTAAGAG CCGGGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACA GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA TTCGGCCTGA GGGGCAGGAC AGTGCCTTCT CCTGGACAGA CATGTTGTTC 
AAGAATAATT CCGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCCAAGT TTTTTGGGGG TTTTGTGCCT GAGATGGTGC TCACCTCTGA TGATCAGCGC 
CTGCTGACCC ATATCACCCT GGAGCTCCAG CACTACCACC AGCTGCTAGA GAAGGTTCGG 
ATCCGGGATG CCTTACGTAG CATCCTCACC ATCTCTCGCC ATGGCAACCA GTACATTCAG 
GTGAACGAGC CCTGGAAGCG GATTAAAGGT GGCGAAGCTG ACAGGCAGCG GGCAGGGACA 
GTGACAGGCT TGGCCGTGAA TATAGCTGCC TTGCTGTCCG TCATGCTCCA GCCTTACATG 
CCTACAGTTA GTGCCACCAT CCAAGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCCCACAA ACTTCCTATG CACCTTACCA GCAGGACACC AGATTGGCAC GGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTCTGA AGCAGCGCTT CAGTGGGGGC 
CAGCTAGAAG AGTCCTTGGC ATTAAAGGCA AAAGCATCCC CCAAGACAGC AGCTGGACTG 
ACAACAGCTG GACCGCAGCA GATCCAAGCA CTGACAGAGG AAGTGACCAA GCAGGGCAAT 
ATTGTCCGAG AATTGAAGGC ACAGAAGGCA GACAAGAACC AGATTGCTGC AGAGGTGGCT 
AAACTCTTGG ATCTGAAGAA ACAGTTGGCT CTAGCTGAGG GGAAACCCCT GGAAACCTCT 
AAAGGCAAGA AGAAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847411.1
CDS	295..3015
Translation	MRLFVSEGAPGSLPVLAAAGRAQGRAELLISTVGPEECVVPFLTRPKVPVLQLDSGNYLFSTSAICRYFFLLSGWEQDDLTNQWLEWEATELQPALSAALYYLVVQGKKGEDVLGPVRRALTHIDHSLSRRSCPFLAGETESLADIVLWGALYPLLQDPSYLPEELGALHSWFQTLSSQEPCQRAAETVLKQQGVLALRPYLQKQPQPSSLEGRLVSNEPEEEELATLSEEEIAVAVAAWEKGLESLPPLRPQQNPVLPVAGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKAMEEGLTPQEICDKYHVIHADIYRWFNISFDFFGRTTTPQQTKITQDIFQRLLARGFVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAIELKKPQCKVCRSCPVVKSSQHLFLDLPKLAARVEEWLEKTLPGSDWTANARFIIRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWEKWWKNPEQVNLYQFMAKDNVPFHGIVFPSSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDMLFKNNSELLNNLGNFINRAGMFVSKFFGGFVPEMVLTSDDQRLLTHITLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGGEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILPTNFLCTLPAGHQIGTVSPLFQKLENDQIESLKQRFSGGQLEESLALKAKASPKTAAGLTTAGPQQIQALTEEVTKQGNIVRELKAQKADKNQIAAEVAKLLDLKKQLALAEGKPLETSKGKKKK

Target ORF information:

RefSeq Version	XM_024991643.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methionyl-tRNA synthetase (MARS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991643.1

ORF Insert Sequence:

ATGAGGTTGT TCGTGAGTGA GGGCGCTCCG GGGAGTCTGC CTGTGCTGGC CGCGGCCGGG 
AGGGCCCAGG GCAGAGCGGA GCTGCTCATC AGCACTGTAG GCCCAGAAGA GTGTGTGGTC 
CCATTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCCACCAGTG CAATCTGTCG ATACTTCTTT CTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACCAACCAGT GGCTGGAATG GGAAGCAACC GAGCTGCAGC CAGCTTTGTC CGCTGCCCTG 
TACTATTTAG TGGTCCAAGG GAAGAAGGGA GAAGATGTTC TTGGCCCAGT TCGGAGAGCC 
CTGACGCATA TTGACCACAG CTTGAGTCGT CGGAGCTGTC CGTTCCTGGC TGGGGAGACA 
GAATCTCTAG CTGATATTGT TTTGTGGGGA GCGCTCTACC CGTTACTCCA AGACCCATCC 
TATCTCCCTG AGGAGCTGGG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TTCTCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAGCAGCAGG GTGTCCTGGC CCTCCGGCCC 
TACCTCCAGA AGCAGCCCCA GCCCAGCTCC CTTGAGGGCA GGCTTGTCAG CAATGAGCCT 
GAGGAGGAGG AACTGGCTAC TCTGTCTGAG GAGGAGATTG CCGTGGCTGT AGCTGCTTGG 
GAGAAGGGCC TGGAAAGCTT GCCCCCTCTT CGGCCGCAGC AGAATCCAGT GTTGCCCGTG 
GCTGGGGAGA GAAATGTGCT CATCACCAGC GCCCTCCCTT ACGTCAACAA CGTCCCCCAC 
CTTGGAAACA TCATCGGCTG TGTGCTCAGT GCCGACGTCT TTGCAAGGTA CTCCCGGCTC 
CGTCAGTGGA ACACCCTCTA CCTGTGTGGG ACAGATGAGT ATGGTACAGC GACAGAGACC 
AAGGCTATGG AGGAGGGACT AACCCCACAG GAGATCTGTG ACAAGTACCA CGTTATCCAC 
GCTGACATCT ACCGCTGGTT TAATATTTCG TTTGACTTTT TTGGTCGGAC TACCACTCCA 
CAGCAGACCA AAATTACCCA GGACATCTTC CAGCGGCTGT TGGCCCGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT TCGGTGTGAG CACTGTGCCC GCTTCCTGGC TGACCGCTTT 
GTGGAGGGTG TATGTCCCTT CTGTGGCTAC GAGGAAGCCC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATCAATGC CATTGAGCTC AAGAAGCCTC AGTGTAAAGT CTGCCGGTCG 
TGCCCTGTGG TGAAATCCTC TCAGCACCTG TTTCTGGACC TGCCTAAGCT GGCAGCGCGG 
GTGGAGGAGT GGTTGGAGAA GACATTGCCT GGCAGTGACT GGACTGCCAA CGCCCGGTTC 
ATCATTCGTT CTTGGCTTCG AGATGGCCTC AAGCCCCGCT GTATAACCCG AGACCTCAAG 
TGGGGAACCC CTGTGCCCTT AGAAGGTTTT GAGGACAAGG TGTTCTATGT CTGGTTTGAT 
GCCACCATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAATGGGA GAAGTGGTGG 
AAGAACCCAG AACAAGTGAA CCTGTATCAG TTCATGGCCA AGGACAATGT TCCCTTCCAT 
GGCATAGTCT TTCCTTCTTC AGCCCTAGGA GCTGAGGACA ACTACACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTATCT GAATTACGAG GATGGGAAAT TCTCTAAGAG CCGGGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACA GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA TTCGGCCTGA GGGGCAGGAC AGTGCCTTCT CCTGGACAGA CATGTTGTTC 
AAGAATAATT CCGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCCAAGT TTTTTGGGGG TTTTGTGCCT GAGATGGTGC TCACCTCTGA TGATCAGCGC 
CTGCTGACCC ATATCACCCT GGAGCTCCAG CACTACCACC AGCTGCTAGA GAAGGTTCGG 
ATCCGGGATG CCTTACGTAG CATCCTCACC ATCTCTCGCC ATGGCAACCA GTACATTCAG 
GTGAACGAGC CCTGGAAGCG GATTAAAGGT GGCGAAGCTG ACAGGCAGCG GGCAGGGACA 
GTGACAGGCT TGGCCGTGAA TATAGCTGCC TTGCTGTCCG TCATGCTCCA GCCTTACATG 
CCTACAGTTA GTGCCACCAT CCAAGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCCCACAA ACTTCCTATG CACCTTACCA GCAGGACACC AGATTGGCAC GGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTCTGA AGCAGCGCTT CAGTGGGGGC 
CAGCTAGAAG AGTCCTTGGC ATTAAAGGCA AAAGCATCCC CCAAGACAGC AGCTGGACTG 
ACAACAGCTG GACCGCAGCA GATCCAAGCA CTGACAGAGG AAGTGACCAA GCAGGGCAAT 
ATTGTCCGAG AATTGAAGGC ACAGAAGGCA GACAAGAACC AGATTGCTGC AGAGGTGGCT 
AAACTCTTGG ATCTGAAGAA ACAGTTGGCT CTAGCTGAGG GGAAACCCCT GGAAACCTCT 
AAAGGCAAGA AGAAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MARS ( XM_010805159.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803461.1 Bos taurus methionyl-tRNA synthetase (MARS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa19420
Clone ID Related Accession (Same CDS sequence)	XM_010805159.2 , XM_024991643.1 , XM_010805159.3
Accession Version	XM_010805159.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methionine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805159.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGT TCGTGAGTGA GGGCGCTCCG GGGAGTCTGC CTGTGCTGGC CGCGGCCGGG 
AGGGCCCAGG GCAGAGCGGA GCTGCTCATC AGCACTGTAG GCCCAGAAGA GTGTGTGGTC 
CCATTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCCACCAGTG CAATCTGTCG ATACTTCTTT CTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACCAACCAGT GGCTGGAATG GGAAGCAACC GAGCTGCAGC CAGCTTTGTC CGCTGCCCTG 
TACTATTTAG TGGTCCAAGG GAAGAAGGGA GAAGATGTTC TTGGCCCAGT TCGGAGAGCC 
CTGACGCATA TTGACCACAG CTTGAGTCGT CGGAGCTGTC CGTTCCTGGC TGGGGAGACA 
GAATCTCTAG CTGATATTGT TTTGTGGGGA GCGCTCTACC CGTTACTCCA AGACCCATCC 
TATCTCCCTG AGGAGCTGGG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TTCTCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAGCAGCAGG GTGTCCTGGC CCTCCGGCCC 
TACCTCCAGA AGCAGCCCCA GCCCAGCTCC CTTGAGGGCA GGCTTGTCAG CAATGAGCCT 
GAGGAGGAGG AACTGGCTAC TCTGTCTGAG GAGGAGATTG CCGTGGCTGT AGCTGCTTGG 
GAGAAGGGCC TGGAAAGCTT GCCCCCTCTT CGGCCGCAGC AGAATCCAGT GTTGCCCGTG 
GCTGGGGAGA GAAATGTGCT CATCACCAGC GCCCTCCCTT ACGTCAACAA CGTCCCCCAC 
CTTGGAAACA TCATCGGCTG TGTGCTCAGT GCCGACGTCT TTGCAAGGTA CTCCCGGCTC 
CGTCAGTGGA ACACCCTCTA CCTGTGTGGG ACAGATGAGT ATGGTACAGC GACAGAGACC 
AAGGCTATGG AGGAGGGACT AACCCCACAG GAGATCTGTG ACAAGTACCA CGTTATCCAC 
GCTGACATCT ACCGCTGGTT TAATATTTCG TTTGACTTTT TTGGTCGGAC TACCACTCCA 
CAGCAGACCA AAATTACCCA GGACATCTTC CAGCGGCTGT TGGCCCGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT TCGGTGTGAG CACTGTGCCC GCTTCCTGGC TGACCGCTTT 
GTGGAGGGTG TATGTCCCTT CTGTGGCTAC GAGGAAGCCC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATCAATGC CATTGAGCTC AAGAAGCCTC AGTGTAAAGT CTGCCGGTCG 
TGCCCTGTGG TGAAATCCTC TCAGCACCTG TTTCTGGACC TGCCTAAGCT GGCAGCGCGG 
GTGGAGGAGT GGTTGGAGAA GACATTGCCT GGCAGTGACT GGACTGCCAA CGCCCGGTTC 
ATCATTCGTT CTTGGCTTCG AGATGGCCTC AAGCCCCGCT GTATAACCCG AGACCTCAAG 
TGGGGAACCC CTGTGCCCTT AGAAGGTTTT GAGGACAAGG TGTTCTATGT CTGGTTTGAT 
GCCACCATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAATGGGA GAAGTGGTGG 
AAGAACCCAG AACAAGTGAA CCTGTATCAG TTCATGGCCA AGGACAATGT TCCCTTCCAT 
GGCATAGTCT TTCCTTCTTC AGCCCTAGGA GCTGAGGACA ACTACACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTATCT GAATTACGAG GATGGGAAAT TCTCTAAGAG CCGGGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACA GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA TTCGGCCTGA GGGGCAGGAC AGTGCCTTCT CCTGGACAGA CATGTTGTTC 
AAGAATAATT CCGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCCAAGT TTTTTGGGGG TTTTGTGCCT GAGATGGTGC TCACCTCTGA TGATCAGCGC 
CTGCTGACCC ATATCACCCT GGAGCTCCAG CACTACCACC AGCTGCTAGA GAAGGTTCGG 
ATCCGGGATG CCTTACGTAG CATCCTCACC ATCTCTCGCC ATGGCAACCA GTACATTCAG 
GTGAACGAGC CCTGGAAGCG GATTAAAGGT GGCGAAGCTG ACAGGCAGCG GGCAGGGACA 
GTGACAGGCT TGGCCGTGAA TATAGCTGCC TTGCTGTCCG TCATGCTCCA GCCTTACATG 
CCTACAGTTA GTGCCACCAT CCAAGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCCCACAA ACTTCCTATG CACCTTACCA GCAGGACACC AGATTGGCAC GGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTCTGA AGCAGCGCTT CAGTGGGGGC 
CAGCTAGAAG AGTCCTTGGC ATTAAAGGCA AAAGCATCCC CCAAGACAGC AGCTGGACTG 
ACAACAGCTG GACCGCAGCA GATCCAAGCA CTGACAGAGG AAGTGACCAA GCAGGGCAAT 
ATTGTCCGAG AATTGAAGGC ACAGAAGGCA GACAAGAACC AGATTGCTGC AGAGGTGGCT 
AAACTCTTGG ATCTGAAGAA ACAGTTGGCT CTAGCTGAGG GGAAACCCCT GGAAACCTCT 
AAAGGCAAGA AGAAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803461.1
CDS	295..3015
Translation	MRLFVSEGAPGSLPVLAAAGRAQGRAELLISTVGPEECVVPFLTRPKVPVLQLDSGNYLFSTSAICRYFFLLSGWEQDDLTNQWLEWEATELQPALSAALYYLVVQGKKGEDVLGPVRRALTHIDHSLSRRSCPFLAGETESLADIVLWGALYPLLQDPSYLPEELGALHSWFQTLSSQEPCQRAAETVLKQQGVLALRPYLQKQPQPSSLEGRLVSNEPEEEELATLSEEEIAVAVAAWEKGLESLPPLRPQQNPVLPVAGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKAMEEGLTPQEICDKYHVIHADIYRWFNISFDFFGRTTTPQQTKITQDIFQRLLARGFVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAIELKKPQCKVCRSCPVVKSSQHLFLDLPKLAARVEEWLEKTLPGSDWTANARFIIRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWEKWWKNPEQVNLYQFMAKDNVPFHGIVFPSSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDMLFKNNSELLNNLGNFINRAGMFVSKFFGGFVPEMVLTSDDQRLLTHITLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGGEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILPTNFLCTLPAGHQIGTVSPLFQKLENDQIESLKQRFSGGQLEESLALKAKASPKTAAGLTTAGPQQIQALTEEVTKQGNIVRELKAQKADKNQIAAEVAKLLDLKKQLALAEGKPLETSKGKKKK

Target ORF information:

RefSeq Version	XM_010805159.3
Organism	Bos taurus(cattle)
Definition	Bos taurus methionyl-tRNA synthetase (MARS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805159.3

ORF Insert Sequence:

ATGAGGTTGT TCGTGAGTGA GGGCGCTCCG GGGAGTCTGC CTGTGCTGGC CGCGGCCGGG 
AGGGCCCAGG GCAGAGCGGA GCTGCTCATC AGCACTGTAG GCCCAGAAGA GTGTGTGGTC 
CCATTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCCACCAGTG CAATCTGTCG ATACTTCTTT CTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACCAACCAGT GGCTGGAATG GGAAGCAACC GAGCTGCAGC CAGCTTTGTC CGCTGCCCTG 
TACTATTTAG TGGTCCAAGG GAAGAAGGGA GAAGATGTTC TTGGCCCAGT TCGGAGAGCC 
CTGACGCATA TTGACCACAG CTTGAGTCGT CGGAGCTGTC CGTTCCTGGC TGGGGAGACA 
GAATCTCTAG CTGATATTGT TTTGTGGGGA GCGCTCTACC CGTTACTCCA AGACCCATCC 
TATCTCCCTG AGGAGCTGGG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TTCTCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAGCAGCAGG GTGTCCTGGC CCTCCGGCCC 
TACCTCCAGA AGCAGCCCCA GCCCAGCTCC CTTGAGGGCA GGCTTGTCAG CAATGAGCCT 
GAGGAGGAGG AACTGGCTAC TCTGTCTGAG GAGGAGATTG CCGTGGCTGT AGCTGCTTGG 
GAGAAGGGCC TGGAAAGCTT GCCCCCTCTT CGGCCGCAGC AGAATCCAGT GTTGCCCGTG 
GCTGGGGAGA GAAATGTGCT CATCACCAGC GCCCTCCCTT ACGTCAACAA CGTCCCCCAC 
CTTGGAAACA TCATCGGCTG TGTGCTCAGT GCCGACGTCT TTGCAAGGTA CTCCCGGCTC 
CGTCAGTGGA ACACCCTCTA CCTGTGTGGG ACAGATGAGT ATGGTACAGC GACAGAGACC 
AAGGCTATGG AGGAGGGACT AACCCCACAG GAGATCTGTG ACAAGTACCA CGTTATCCAC 
GCTGACATCT ACCGCTGGTT TAATATTTCG TTTGACTTTT TTGGTCGGAC TACCACTCCA 
CAGCAGACCA AAATTACCCA GGACATCTTC CAGCGGCTGT TGGCCCGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT TCGGTGTGAG CACTGTGCCC GCTTCCTGGC TGACCGCTTT 
GTGGAGGGTG TATGTCCCTT CTGTGGCTAC GAGGAAGCCC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATCAATGC CATTGAGCTC AAGAAGCCTC AGTGTAAAGT CTGCCGGTCG 
TGCCCTGTGG TGAAATCCTC TCAGCACCTG TTTCTGGACC TGCCTAAGCT GGCAGCGCGG 
GTGGAGGAGT GGTTGGAGAA GACATTGCCT GGCAGTGACT GGACTGCCAA CGCCCGGTTC 
ATCATTCGTT CTTGGCTTCG AGATGGCCTC AAGCCCCGCT GTATAACCCG AGACCTCAAG 
TGGGGAACCC CTGTGCCCTT AGAAGGTTTT GAGGACAAGG TGTTCTATGT CTGGTTTGAT 
GCCACCATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAATGGGA GAAGTGGTGG 
AAGAACCCAG AACAAGTGAA CCTGTATCAG TTCATGGCCA AGGACAATGT TCCCTTCCAT 
GGCATAGTCT TTCCTTCTTC AGCCCTAGGA GCTGAGGACA ACTACACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTATCT GAATTACGAG GATGGGAAAT TCTCTAAGAG CCGGGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACA GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA TTCGGCCTGA GGGGCAGGAC AGTGCCTTCT CCTGGACAGA CATGTTGTTC 
AAGAATAATT CCGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCCAAGT TTTTTGGGGG TTTTGTGCCT GAGATGGTGC TCACCTCTGA TGATCAGCGC 
CTGCTGACCC ATATCACCCT GGAGCTCCAG CACTACCACC AGCTGCTAGA GAAGGTTCGG 
ATCCGGGATG CCTTACGTAG CATCCTCACC ATCTCTCGCC ATGGCAACCA GTACATTCAG 
GTGAACGAGC CCTGGAAGCG GATTAAAGGT GGCGAAGCTG ACAGGCAGCG GGCAGGGACA 
GTGACAGGCT TGGCCGTGAA TATAGCTGCC TTGCTGTCCG TCATGCTCCA GCCTTACATG 
CCTACAGTTA GTGCCACCAT CCAAGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCCCACAA ACTTCCTATG CACCTTACCA GCAGGACACC AGATTGGCAC GGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTCTGA AGCAGCGCTT CAGTGGGGGC 
CAGCTAGAAG AGTCCTTGGC ATTAAAGGCA AAAGCATCCC CCAAGACAGC AGCTGGACTG 
ACAACAGCTG GACCGCAGCA GATCCAAGCA CTGACAGAGG AAGTGACCAA GCAGGGCAAT 
ATTGTCCGAG AATTGAAGGC ACAGAAGGCA GACAAGAACC AGATTGCTGC AGAGGTGGCT 
AAACTCTTGG ATCTGAAGAA ACAGTTGGCT CTAGCTGAGG GGAAACCCCT GGAAACCTCT 
AAAGGCAAGA AGAAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MARS ( XM_015471086.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326572.1 Bos taurus methionyl-tRNA synthetase (MARS), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa90749
Clone ID Related Accession (Same CDS sequence)	XM_015471086.2 , XM_015471086.1
Accession Version	XM_015471086.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methionine--tRNA ligase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGT TCGTGAGTGA GGGCGCTCCG GGGAGTCTGC CTGTGCTGGC CGCGGCCGGG 
AGGGCCCAGG GCAGAGCGGA GCTGCTCATC AGCACTGTAG GCCCAGAAGA GTGTGTGGTC 
CCATTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCCACCAGTG CAATCTGTCG ATACTTCTTT CTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACCAACCAGT GGCTGGAATG GGAAGCAACC GAGCTGCAGC CAGCTTTGTC CGCTGCCCTG 
TACTATTTAG TGGTCCAAGG GAAGAAGGGA GAAGATGTTC TTGGCCCAGT TCGGAGAGCC 
CTGACGCATA TTGACCACAG CTTGAGTCGT CGGAGCTGTC CGTTCCTGGC TGGGGAGACA 
GAATCTCTAG CTGATATTGT TTTGTGGGGA GCGCTCTACC CGTTACTCCA AGACCCATCC 
TATCTCCCTG AGGAGCTGGG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TTCTCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAGCAGCAGG GTGTCCTGGC CCTCCGGCCC 
TACCTCCAGA AGCAGCCCCA GCCCAGCTCC CTTGAGGGCA GGCTTGTCAG CAATGAGCCT 
GAGGAGGAGG AACTGGCTAC TCTGTCTGAG GAGGAGATTG CCGTGGCTGT AGCTGCTTGG 
GAGAAGGGCC TGGAAAGCTT GCCCCCTCTT CGGCCGCAGC AGAATCCAGT GTTGCCCGTG 
GCTGGGGAGA GAAATGTGCT CATCACCAGC GCCCTCCCTT ACGTCAACAA CGTCCCCCAC 
CTTGGAAACA TCATCGGCTG TGTGCTCAGT GCCGACGTCT TTGCAAGGTA CTCCCGGCTC 
CGTCAGTGGA ACACCCTCTA CCTGTGTGGG ACAGATGAGT ATGGTACAGC GACAGAGACC 
AAGGCTATGG AGGAGGGACT AACCCCACAG GAGATCTGTG ACAAGTACCA CGTTATCCAC 
GCTGACATCT ACCGCTGGTT TAATATTTCG TTTGACTTTT TTGGTCGGAC TACCACTCCA 
CAGCAGACCA AAATTACCCA GGACATCTTC CAGCGGCTGT TGGCCCGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT TCGGTGTGAG CACTGTGCCC GCTTCCTGGC TGACCGCTTT 
GTGGAGGGTG TATGTCCCTT CTGTGGCTAC GAGGAAGCCC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATCAATGC CATTGAGCTC AAGAAGCCTC AGTGTAAAGT CTGCCGGTCG 
TGCCCTGTGG TGAAATCCTC TCAGCACCTG TTTCTGGACC TGCCTAAGCT GGCAGCGCGG 
GTGGAGGAGT GGTTGGAGAA GACATTGCCT GGCAGTGACT GGACTGCCAA CGCCCGGTTC 
ATCATTCGTT CTTGGCTTCG AGATGGCCTC AAGCCCCGCT GTATAACCCG AGACCTCAAG 
TGGGGAACCC CTGTGCCCTT AGAAGGTTTT GAGGACAAGG TGTTCTATGT CTGGTTTGAT 
GCCACCATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAATGGGA GAAGTGGTGG 
AAGAACCCAG AACAAGTGAA CCTGTATCAG TTCATGGCCA AGGACAATGT TCCCTTCCAT 
GGCATAGTCT TTCCTTCTTC AGCCCTAGGA GCTGAGGACA ACTACACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTATCT GAATTACGAG GATGGGAAAT TCTCTAAGAG CCGGGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACA GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA TTCGGCCTGA GGGGCAGGAC AGTGCCTTCT CCTGGACAGA CATGTTGTTC 
AAGAATAATT CCGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCCAAGT TTTTTGGGGG TTTTGTGCCT GAGATGGTGC TCACCTCTGA TGATCAGCGC 
CTGCTGACCC ATATCACCCT GGAGCTCCAG CACTACCACC AGCTGCTAGA GAAGGTTCGG 
ATCCGGGATG CCTTACGTAG CATCCTCACC ATCTCTCGCC ATGGCAACCA GTACATTCAG 
GTGAACGAGC CCTGGAAGCG GATTAAAGGT GGCGAAGCTG ACAGGCAGCG GGCAGGGACA 
GTGACAGGCT TGGCCGTGAA TATAGCTGCC TTGCTGTCCG TCATGCTCCA GCCTTACATG 
CCTACAGTTA GTGCCACCAT CCAAGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCCCACAA ACTTCCTATG CACCTTACCA GCAGGACACC AGATTGGCAC GGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTCTGA AGCAGCGCTT CAGTGGGGGC 
CAGGCAAAAG CATCCCCCAA GACAGCAGCT GGACTGACAA CAGCTGGACC GCAGCAGATC 
CAAGCACTGA CAGAGGAAGT GACCAAGCAG GGCAATATTG TCCGAGAATT GAAGGCACAG 
AAGGCAGACA AGAACCAGAT TGCTGCAGAG GTGGCTAAAC TCTTGGATCT GAAGAAACAG 
TTGGCTCTAG CTGAGGGGAA ACCCCTGGAA ACCTCTAAAG GCAAGAAGAA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326572.1
CDS	295..2991
Translation	MRLFVSEGAPGSLPVLAAAGRAQGRAELLISTVGPEECVVPFLTRPKVPVLQLDSGNYLFSTSAICRYFFLLSGWEQDDLTNQWLEWEATELQPALSAALYYLVVQGKKGEDVLGPVRRALTHIDHSLSRRSCPFLAGETESLADIVLWGALYPLLQDPSYLPEELGALHSWFQTLSSQEPCQRAAETVLKQQGVLALRPYLQKQPQPSSLEGRLVSNEPEEEELATLSEEEIAVAVAAWEKGLESLPPLRPQQNPVLPVAGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKAMEEGLTPQEICDKYHVIHADIYRWFNISFDFFGRTTTPQQTKITQDIFQRLLARGFVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAIELKKPQCKVCRSCPVVKSSQHLFLDLPKLAARVEEWLEKTLPGSDWTANARFIIRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWEKWWKNPEQVNLYQFMAKDNVPFHGIVFPSSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDMLFKNNSELLNNLGNFINRAGMFVSKFFGGFVPEMVLTSDDQRLLTHITLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGGEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILPTNFLCTLPAGHQIGTVSPLFQKLENDQIESLKQRFSGGQAKASPKTAAGLTTAGPQQIQALTEEVTKQGNIVRELKAQKADKNQIAAEVAKLLDLKKQLALAEGKPLETSKGKKKK

Target ORF information:

RefSeq Version	XM_015471086.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methionyl-tRNA synthetase (MARS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471086.2

ORF Insert Sequence:

ATGAGGTTGT TCGTGAGTGA GGGCGCTCCG GGGAGTCTGC CTGTGCTGGC CGCGGCCGGG 
AGGGCCCAGG GCAGAGCGGA GCTGCTCATC AGCACTGTAG GCCCAGAAGA GTGTGTGGTC 
CCATTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCCACCAGTG CAATCTGTCG ATACTTCTTT CTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACCAACCAGT GGCTGGAATG GGAAGCAACC GAGCTGCAGC CAGCTTTGTC CGCTGCCCTG 
TACTATTTAG TGGTCCAAGG GAAGAAGGGA GAAGATGTTC TTGGCCCAGT TCGGAGAGCC 
CTGACGCATA TTGACCACAG CTTGAGTCGT CGGAGCTGTC CGTTCCTGGC TGGGGAGACA 
GAATCTCTAG CTGATATTGT TTTGTGGGGA GCGCTCTACC CGTTACTCCA AGACCCATCC 
TATCTCCCTG AGGAGCTGGG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TTCTCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAGCAGCAGG GTGTCCTGGC CCTCCGGCCC 
TACCTCCAGA AGCAGCCCCA GCCCAGCTCC CTTGAGGGCA GGCTTGTCAG CAATGAGCCT 
GAGGAGGAGG AACTGGCTAC TCTGTCTGAG GAGGAGATTG CCGTGGCTGT AGCTGCTTGG 
GAGAAGGGCC TGGAAAGCTT GCCCCCTCTT CGGCCGCAGC AGAATCCAGT GTTGCCCGTG 
GCTGGGGAGA GAAATGTGCT CATCACCAGC GCCCTCCCTT ACGTCAACAA CGTCCCCCAC 
CTTGGAAACA TCATCGGCTG TGTGCTCAGT GCCGACGTCT TTGCAAGGTA CTCCCGGCTC 
CGTCAGTGGA ACACCCTCTA CCTGTGTGGG ACAGATGAGT ATGGTACAGC GACAGAGACC 
AAGGCTATGG AGGAGGGACT AACCCCACAG GAGATCTGTG ACAAGTACCA CGTTATCCAC 
GCTGACATCT ACCGCTGGTT TAATATTTCG TTTGACTTTT TTGGTCGGAC TACCACTCCA 
CAGCAGACCA AAATTACCCA GGACATCTTC CAGCGGCTGT TGGCCCGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT TCGGTGTGAG CACTGTGCCC GCTTCCTGGC TGACCGCTTT 
GTGGAGGGTG TATGTCCCTT CTGTGGCTAC GAGGAAGCCC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATCAATGC CATTGAGCTC AAGAAGCCTC AGTGTAAAGT CTGCCGGTCG 
TGCCCTGTGG TGAAATCCTC TCAGCACCTG TTTCTGGACC TGCCTAAGCT GGCAGCGCGG 
GTGGAGGAGT GGTTGGAGAA GACATTGCCT GGCAGTGACT GGACTGCCAA CGCCCGGTTC 
ATCATTCGTT CTTGGCTTCG AGATGGCCTC AAGCCCCGCT GTATAACCCG AGACCTCAAG 
TGGGGAACCC CTGTGCCCTT AGAAGGTTTT GAGGACAAGG TGTTCTATGT CTGGTTTGAT 
GCCACCATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAATGGGA GAAGTGGTGG 
AAGAACCCAG AACAAGTGAA CCTGTATCAG TTCATGGCCA AGGACAATGT TCCCTTCCAT 
GGCATAGTCT TTCCTTCTTC AGCCCTAGGA GCTGAGGACA ACTACACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTATCT GAATTACGAG GATGGGAAAT TCTCTAAGAG CCGGGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACA GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA TTCGGCCTGA GGGGCAGGAC AGTGCCTTCT CCTGGACAGA CATGTTGTTC 
AAGAATAATT CCGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCCAAGT TTTTTGGGGG TTTTGTGCCT GAGATGGTGC TCACCTCTGA TGATCAGCGC 
CTGCTGACCC ATATCACCCT GGAGCTCCAG CACTACCACC AGCTGCTAGA GAAGGTTCGG 
ATCCGGGATG CCTTACGTAG CATCCTCACC ATCTCTCGCC ATGGCAACCA GTACATTCAG 
GTGAACGAGC CCTGGAAGCG GATTAAAGGT GGCGAAGCTG ACAGGCAGCG GGCAGGGACA 
GTGACAGGCT TGGCCGTGAA TATAGCTGCC TTGCTGTCCG TCATGCTCCA GCCTTACATG 
CCTACAGTTA GTGCCACCAT CCAAGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCCCACAA ACTTCCTATG CACCTTACCA GCAGGACACC AGATTGGCAC GGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTCTGA AGCAGCGCTT CAGTGGGGGC 
CAGGCAAAAG CATCCCCCAA GACAGCAGCT GGACTGACAA CAGCTGGACC GCAGCAGATC 
CAAGCACTGA CAGAGGAAGT GACCAAGCAG GGCAATATTG TCCGAGAATT GAAGGCACAG 
AAGGCAGACA AGAACCAGAT TGCTGCAGAG GTGGCTAAAC TCTTGGATCT GAAGAAACAG 
TTGGCTCTAG CTGAGGGGAA ACCCCTGGAA ACCTCTAAAG GCAAGAAGAA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MARS ( XM_015471086.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326572.1 Bos taurus methionyl-tRNA synthetase (MARS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa90749
Clone ID Related Accession (Same CDS sequence)	XM_015471086.2 , XM_015471086.1
Accession Version	XM_015471086.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methionine--tRNA ligase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGT TCGTGAGTGA GGGCGCTCCG GGGAGTCTGC CTGTGCTGGC CGCGGCCGGG 
AGGGCCCAGG GCAGAGCGGA GCTGCTCATC AGCACTGTAG GCCCAGAAGA GTGTGTGGTC 
CCATTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCCACCAGTG CAATCTGTCG ATACTTCTTT CTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACCAACCAGT GGCTGGAATG GGAAGCAACC GAGCTGCAGC CAGCTTTGTC CGCTGCCCTG 
TACTATTTAG TGGTCCAAGG GAAGAAGGGA GAAGATGTTC TTGGCCCAGT TCGGAGAGCC 
CTGACGCATA TTGACCACAG CTTGAGTCGT CGGAGCTGTC CGTTCCTGGC TGGGGAGACA 
GAATCTCTAG CTGATATTGT TTTGTGGGGA GCGCTCTACC CGTTACTCCA AGACCCATCC 
TATCTCCCTG AGGAGCTGGG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TTCTCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAGCAGCAGG GTGTCCTGGC CCTCCGGCCC 
TACCTCCAGA AGCAGCCCCA GCCCAGCTCC CTTGAGGGCA GGCTTGTCAG CAATGAGCCT 
GAGGAGGAGG AACTGGCTAC TCTGTCTGAG GAGGAGATTG CCGTGGCTGT AGCTGCTTGG 
GAGAAGGGCC TGGAAAGCTT GCCCCCTCTT CGGCCGCAGC AGAATCCAGT GTTGCCCGTG 
GCTGGGGAGA GAAATGTGCT CATCACCAGC GCCCTCCCTT ACGTCAACAA CGTCCCCCAC 
CTTGGAAACA TCATCGGCTG TGTGCTCAGT GCCGACGTCT TTGCAAGGTA CTCCCGGCTC 
CGTCAGTGGA ACACCCTCTA CCTGTGTGGG ACAGATGAGT ATGGTACAGC GACAGAGACC 
AAGGCTATGG AGGAGGGACT AACCCCACAG GAGATCTGTG ACAAGTACCA CGTTATCCAC 
GCTGACATCT ACCGCTGGTT TAATATTTCG TTTGACTTTT TTGGTCGGAC TACCACTCCA 
CAGCAGACCA AAATTACCCA GGACATCTTC CAGCGGCTGT TGGCCCGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT TCGGTGTGAG CACTGTGCCC GCTTCCTGGC TGACCGCTTT 
GTGGAGGGTG TATGTCCCTT CTGTGGCTAC GAGGAAGCCC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATCAATGC CATTGAGCTC AAGAAGCCTC AGTGTAAAGT CTGCCGGTCG 
TGCCCTGTGG TGAAATCCTC TCAGCACCTG TTTCTGGACC TGCCTAAGCT GGCAGCGCGG 
GTGGAGGAGT GGTTGGAGAA GACATTGCCT GGCAGTGACT GGACTGCCAA CGCCCGGTTC 
ATCATTCGTT CTTGGCTTCG AGATGGCCTC AAGCCCCGCT GTATAACCCG AGACCTCAAG 
TGGGGAACCC CTGTGCCCTT AGAAGGTTTT GAGGACAAGG TGTTCTATGT CTGGTTTGAT 
GCCACCATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAATGGGA GAAGTGGTGG 
AAGAACCCAG AACAAGTGAA CCTGTATCAG TTCATGGCCA AGGACAATGT TCCCTTCCAT 
GGCATAGTCT TTCCTTCTTC AGCCCTAGGA GCTGAGGACA ACTACACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTATCT GAATTACGAG GATGGGAAAT TCTCTAAGAG CCGGGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACA GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA TTCGGCCTGA GGGGCAGGAC AGTGCCTTCT CCTGGACAGA CATGTTGTTC 
AAGAATAATT CCGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCCAAGT TTTTTGGGGG TTTTGTGCCT GAGATGGTGC TCACCTCTGA TGATCAGCGC 
CTGCTGACCC ATATCACCCT GGAGCTCCAG CACTACCACC AGCTGCTAGA GAAGGTTCGG 
ATCCGGGATG CCTTACGTAG CATCCTCACC ATCTCTCGCC ATGGCAACCA GTACATTCAG 
GTGAACGAGC CCTGGAAGCG GATTAAAGGT GGCGAAGCTG ACAGGCAGCG GGCAGGGACA 
GTGACAGGCT TGGCCGTGAA TATAGCTGCC TTGCTGTCCG TCATGCTCCA GCCTTACATG 
CCTACAGTTA GTGCCACCAT CCAAGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCCCACAA ACTTCCTATG CACCTTACCA GCAGGACACC AGATTGGCAC GGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTCTGA AGCAGCGCTT CAGTGGGGGC 
CAGGCAAAAG CATCCCCCAA GACAGCAGCT GGACTGACAA CAGCTGGACC GCAGCAGATC 
CAAGCACTGA CAGAGGAAGT GACCAAGCAG GGCAATATTG TCCGAGAATT GAAGGCACAG 
AAGGCAGACA AGAACCAGAT TGCTGCAGAG GTGGCTAAAC TCTTGGATCT GAAGAAACAG 
TTGGCTCTAG CTGAGGGGAA ACCCCTGGAA ACCTCTAAAG GCAAGAAGAA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326572.1
CDS	242..2938
Translation	MRLFVSEGAPGSLPVLAAAGRAQGRAELLISTVGPEECVVPFLTRPKVPVLQLDSGNYLFSTSAICRYFFLLSGWEQDDLTNQWLEWEATELQPALSAALYYLVVQGKKGEDVLGPVRRALTHIDHSLSRRSCPFLAGETESLADIVLWGALYPLLQDPSYLPEELGALHSWFQTLSSQEPCQRAAETVLKQQGVLALRPYLQKQPQPSSLEGRLVSNEPEEEELATLSEEEIAVAVAAWEKGLESLPPLRPQQNPVLPVAGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKAMEEGLTPQEICDKYHVIHADIYRWFNISFDFFGRTTTPQQTKITQDIFQRLLARGFVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAIELKKPQCKVCRSCPVVKSSQHLFLDLPKLAARVEEWLEKTLPGSDWTANARFIIRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWEKWWKNPEQVNLYQFMAKDNVPFHGIVFPSSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDMLFKNNSELLNNLGNFINRAGMFVSKFFGGFVPEMVLTSDDQRLLTHITLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGGEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILPTNFLCTLPAGHQIGTVSPLFQKLENDQIESLKQRFSGGQAKASPKTAAGLTTAGPQQIQALTEEVTKQGNIVRELKAQKADKNQIAAEVAKLLDLKKQLALAEGKPLETSKGKKKK

Target ORF information:

RefSeq Version	XM_015471086.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methionyl-tRNA synthetase (MARS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471086.1

ORF Insert Sequence:

ATGAGGTTGT TCGTGAGTGA GGGCGCTCCG GGGAGTCTGC CTGTGCTGGC CGCGGCCGGG 
AGGGCCCAGG GCAGAGCGGA GCTGCTCATC AGCACTGTAG GCCCAGAAGA GTGTGTGGTC 
CCATTCCTGA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCCACCAGTG CAATCTGTCG ATACTTCTTT CTGTTATCTG GCTGGGAGCA AGATGACCTC 
ACCAACCAGT GGCTGGAATG GGAAGCAACC GAGCTGCAGC CAGCTTTGTC CGCTGCCCTG 
TACTATTTAG TGGTCCAAGG GAAGAAGGGA GAAGATGTTC TTGGCCCAGT TCGGAGAGCC 
CTGACGCATA TTGACCACAG CTTGAGTCGT CGGAGCTGTC CGTTCCTGGC TGGGGAGACA 
GAATCTCTAG CTGATATTGT TTTGTGGGGA GCGCTCTACC CGTTACTCCA AGACCCATCC 
TATCTCCCTG AGGAGCTGGG TGCCCTGCAC AGCTGGTTCC AGACACTGAG TTCTCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAGCAGCAGG GTGTCCTGGC CCTCCGGCCC 
TACCTCCAGA AGCAGCCCCA GCCCAGCTCC CTTGAGGGCA GGCTTGTCAG CAATGAGCCT 
GAGGAGGAGG AACTGGCTAC TCTGTCTGAG GAGGAGATTG CCGTGGCTGT AGCTGCTTGG 
GAGAAGGGCC TGGAAAGCTT GCCCCCTCTT CGGCCGCAGC AGAATCCAGT GTTGCCCGTG 
GCTGGGGAGA GAAATGTGCT CATCACCAGC GCCCTCCCTT ACGTCAACAA CGTCCCCCAC 
CTTGGAAACA TCATCGGCTG TGTGCTCAGT GCCGACGTCT TTGCAAGGTA CTCCCGGCTC 
CGTCAGTGGA ACACCCTCTA CCTGTGTGGG ACAGATGAGT ATGGTACAGC GACAGAGACC 
AAGGCTATGG AGGAGGGACT AACCCCACAG GAGATCTGTG ACAAGTACCA CGTTATCCAC 
GCTGACATCT ACCGCTGGTT TAATATTTCG TTTGACTTTT TTGGTCGGAC TACCACTCCA 
CAGCAGACCA AAATTACCCA GGACATCTTC CAGCGGCTGT TGGCCCGAGG TTTTGTGCTG 
CAAGATACTG TGGAGCAACT TCGGTGTGAG CACTGTGCCC GCTTCCTGGC TGACCGCTTT 
GTGGAGGGTG TATGTCCCTT CTGTGGCTAC GAGGAAGCCC GGGGTGACCA GTGTGACAAG 
TGTGGCAAGC TCATCAATGC CATTGAGCTC AAGAAGCCTC AGTGTAAAGT CTGCCGGTCG 
TGCCCTGTGG TGAAATCCTC TCAGCACCTG TTTCTGGACC TGCCTAAGCT GGCAGCGCGG 
GTGGAGGAGT GGTTGGAGAA GACATTGCCT GGCAGTGACT GGACTGCCAA CGCCCGGTTC 
ATCATTCGTT CTTGGCTTCG AGATGGCCTC AAGCCCCGCT GTATAACCCG AGACCTCAAG 
TGGGGAACCC CTGTGCCCTT AGAAGGTTTT GAGGACAAGG TGTTCTATGT CTGGTTTGAT 
GCCACCATTG GCTATCTGTC CATCACAGCC AACTACACAG ACCAATGGGA GAAGTGGTGG 
AAGAACCCAG AACAAGTGAA CCTGTATCAG TTCATGGCCA AGGACAATGT TCCCTTCCAT 
GGCATAGTCT TTCCTTCTTC AGCCCTAGGA GCTGAGGACA ACTACACCTT GGTCAGCCAC 
CTCATTGCTA CAGAGTATCT GAATTACGAG GATGGGAAAT TCTCTAAGAG CCGGGGTGTG 
GGAGTGTTTG GGGACATGGC CCAGGACACA GGGATCCCTG CTGACATCTG GCGCTTCTAT 
CTGCTGTACA TTCGGCCTGA GGGGCAGGAC AGTGCCTTCT CCTGGACAGA CATGTTGTTC 
AAGAATAATT CCGAGCTGCT TAACAACCTG GGCAACTTCA TCAACAGAGC TGGGATGTTT 
GTGTCCAAGT TTTTTGGGGG TTTTGTGCCT GAGATGGTGC TCACCTCTGA TGATCAGCGC 
CTGCTGACCC ATATCACCCT GGAGCTCCAG CACTACCACC AGCTGCTAGA GAAGGTTCGG 
ATCCGGGATG CCTTACGTAG CATCCTCACC ATCTCTCGCC ATGGCAACCA GTACATTCAG 
GTGAACGAGC CCTGGAAGCG GATTAAAGGT GGCGAAGCTG ACAGGCAGCG GGCAGGGACA 
GTGACAGGCT TGGCCGTGAA TATAGCTGCC TTGCTGTCCG TCATGCTCCA GCCTTACATG 
CCTACAGTTA GTGCCACCAT CCAAGCCCAG CTGCAGCTCC CACCTCCAGC CTGCAGTATC 
CTGCCCACAA ACTTCCTATG CACCTTACCA GCAGGACACC AGATTGGCAC GGTCAGTCCC 
TTGTTCCAAA AATTGGAAAA TGACCAGATT GAAAGTCTGA AGCAGCGCTT CAGTGGGGGC 
CAGGCAAAAG CATCCCCCAA GACAGCAGCT GGACTGACAA CAGCTGGACC GCAGCAGATC 
CAAGCACTGA CAGAGGAAGT GACCAAGCAG GGCAATATTG TCCGAGAATT GAAGGCACAG 
AAGGCAGACA AGAACCAGAT TGCTGCAGAG GTGGCTAAAC TCTTGGATCT GAAGAAACAG 
TTGGCTCTAG CTGAGGGGAA ACCCCTGGAA ACCTCTAAAG GCAAGAAGAA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MARS cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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