Mars1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mars1
Entrez Gene ID	216443
Full Name	methionine-tRNA synthetase
Synonyms	Metrs,Mtrns
General protein information	Preferred Names methionine-tRNA synthetase Names methionine--tRNA ligase, cytoplasmic methionine tRNA ligase methionyl-tRNA synthetase, cytoplasmic
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 D3
Summary	The encoded protein belongs to the class I family of tRNA synthetases, a class of enzymes that charge tRNAs with their cognate amino acids. The related human gene product is essential for the translation initiation of mRNAs. This gene has an overlapping 3' UTR tail-to-tail arrangement with an adjacent gene on the opposite strand that encodes an inhibitor of the CCAAT/enhancer-binding protein's DNA binding activity. This arrangement, conserved in human and mouse, may be involved in mRNA stability and possible functional and regulatory interaction of these adjacent overlapping genes. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2010].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006513568.4	XP_006513631.1	methionine--tRNA ligase, cytoplasmic isoform X1
NM_001171582.1	NP_001165053.1	methionine--tRNA ligase, cytoplasmic isoform 1
XM_006513568.3	XP_006513631.1	methionine--tRNA ligase, cytoplasmic isoform X1
NM_001003913.2	NP_001003913.1	methionine--tRNA ligase, cytoplasmic isoform 2

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0A09647g	XP_451421.1
Bos taurus (cattle)	MARS	NP_001033180.1
Mus musculus (house mouse)	Mars	NP_001165053.1
Rattus norvegicus (Norway rat)	Mars	NP_001121131.1
Schizosaccharomyces pombe (fission yeast)	rar1	NP_595586.1
Homo sapiens (human)	MARS	NP_004981.2
Pan troglodytes (chimpanzee)	MARS	XP_509164.2
	MARS	XP_001116063.1
Canis lupus familiaris (dog)	MARS	XP_531646.2
Gallus gallus (chicken)	MARS	NP_001244301.1
Danio rerio (zebrafish)	mars	NP_956370.1
Eremothecium gossypii	AGOS_AGR332C	NP_986998.1
Xenopus tropicalis (tropical clawed frog)	mars	NP_001017000.2
Drosophila melanogaster (fruit fly)	CG15100	NP_611382.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007891	XP_317601.4
Neurospora crassa	NCU07451	XP_964638.1
Saccharomyces cerevisiae (baker's yeast)	MES1	NP_011780.3
Magnaporthe oryzae (rice blast fungus)	MGG_01046	XP_003717896.1
Oryza sativa (rice)	Os10g0400100	NP_001064541.1

Gene Ontology
Bibliography

Related articles in PubMed

p38 is essential for the assembly and stability of macromolecular tRNA synthetase complex: implications for its physiological significance.
Kim JY, Kang YS, Lee JW, Kim HJ, Ahn YH, Park H, Ko YG, Kim S
Proceedings of the National Academy of Sciences of the United States of America99(12)7912-6(2002 Jun)

CHOP/GADD153 and methionyl-tRNA synthetase (MetRS) genes overlap in a conserved region that controls mRNA stability.
Ubeda M, Schmitt-Ney M, Ferrer J, Habener JF
Biochemical and biophysical research communications262(1)31-8(1999 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mars1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mars1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu10532	NM_001003913.2 Latest version!	Mus musculus methionine-tRNA synthetase 1 (Mars1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$531.30 $759.00
OMu44448	XM_006513568.4 Latest version!	Mus musculus methionine-tRNA synthetase (Mars), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu11847	NM_001171582.1 Latest version!	Mus musculus methionine-tRNA synthetase 1 (Mars1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OMu44448	XM_006513568.3	Mus musculus methionine-tRNA synthetase (Mars), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mars1 ( XM_006513568.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513631.1 Mus musculus methionine-tRNA synthetase (Mars), transcript variant X1, mRNA.

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CloneID	OMu44448
Clone ID Related Accession (Same CDS sequence)	XM_006513568.4 , XM_006513568.3
Accession Version	XM_006513568.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methionine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513568.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GCCTAACCCC ACGGGAAATC TGTGACAAGT ACCATGCCAT CCATGCTGAC 
ATCTACCGCT GGTTCGGCAT ATCGTTCGAT ACTTTCGGGC GCACTACCAC TCCTCAGCAG 
ACCAAAATCA CCCAGGACAT CTTCCAGAGG TTGCTGACCC GGGGGTTTGT GCTGCGAGAT 
ACTGTGGAGC AGCTTCGGTG TGAGCGGTGT GCACGTTTCC TGGCTGACCG CTTTGTGGAG 
GGTGTGTGTC CCTTCTGTGG CTATGAAGAG GCCCGAGGTG ACCAGTGTGA CAGGTGTGGC 
AAGCTCATCA ATGCCATTGA GCTCAAGAAA CCACAGTGCA AAATCTGCCG CTCCTGCCCT 
GTGGTGAGGT CCTCACAGCA CCTGTTTCTA GACTTGCCTA AGTTGGAAAA GCGTCTGGAG 
GACTGGTTGG GGAAGACAGT GCCTGGCAGT GACTGGACAC CCAATGCCAG GTTCATTATA 
CGTTCCTGGC TTCGAGATGG CCTCAAGCCA CGATGCATCA CCAGAGACCT CAAATGGGGA 
ACGCCTGTGC CCTTGGAAGG TTTTGAGGAC AAGGTATTTT ACGTCTGGTT TGATGCTACT 
ATTGGCTACG TGTCCATCAC AGCCAACTAC ACAGACCAAT GGGAGAAATG GTGGAAGAAC 
CCAGAACAAG TGGACCTTTA CCAGTTCATG GCCAAAGACA ATGTTCCCTT CCATGGCTTG 
GTCTTTCCGT GTTCAGTCCT AGGAGCTGAG GACAACTACA CCCTGGTCAA GCACATCATT 
GCTACAGAGT ACCTGAACTA TGAGGATGGG AAATTCTCTA AGAGCCGGGG CATAGGAGTG 
TTTGGAGACA TGGCCAAGGA TACAGGAATC CCTGCTGACA TCTGGCGATT CTATCTGCTA 
TACATTCGGC CTGAGGGCCA GGACAGTGCC TTCTCCTGGA CAGACTTGTT GATTAAAAAC 
AATTCTGAGC TGCTCAACAA CCTGGGCAAC TTCATCAACA GAGCTGGCAT GTTTGTTTCT 
AAGTTTTTTG GCGGTTGTGT GCCTGAGATG GCGCTAACCC CTGATGACAG ACGCCTGGTG 
GCCCATGTCT CTTGGGAACT CCAGCACTAT CACCAGCTGT TGGAAAAGGT TCGGATCCGG 
GATGCCTTGC GCAGTATCCT CACCATATCT CGCCATGGCA ACCAATACAT TCAAGTGAAT 
GAGCCCTGGA AACGGATTAA AGGTGGTGAG ATGGACAGGC AGCGGGCAGG CACAGTGACA 
GGCATGGCAG TGAACATGGC TGCCTTGCTG TCTGTCATGC TGCAGCCATA CATGCCCACA 
GTCAGCTCTA CCATCCAGAC CCAGCTGCAG CTCCCACCTG CAGCCTGCCG CATCCTTGCC 
ACAAGCTTCA TTTGTACCTT GCCAGCAGGC CACCGAATTG GCACAGTCAG TCCTTTGTTC 
CAAAAACTGG AAAATGACCA GATTGAAAAT TTGAGGCAGC GCTTTGGAGG GGGTCAGCTA 
GAAGAGTCCT TGGAGTTAAA GGCTAAAGGC TCCCCCAAGC CAGCAGCTGT GGAGGCAGTT 
ACAGCAGCAG GCTCGCAGCA CATACAAACG CTGACGGATG AGGTGACCAA GCAGGGCAAC 
GTCGTCCGGG AACTGAAAGC ACAGAAGGCA GACAAGAACC AGGTTGCTGC AGAGGTGGCT 
AAACTCTTGG ATCTAAAGAA ACAGTTGGCT TTGGCTGAGG GGAAACCCAT TGAAACTCCT 
AAAGGCAAGA AGAAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513631.1
CDS	1652..3412
Translation	MEEGLTPREICDKYHAIHADIYRWFGISFDTFGRTTTPQQTKITQDIFQRLLTRGFVLRDTVEQLRCERCARFLADRFVEGVCPFCGYEEARGDQCDRCGKLINAIELKKPQCKICRSCPVVRSSQHLFLDLPKLEKRLEDWLGKTVPGSDWTPNARFIIRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYVSITANYTDQWEKWWKNPEQVDLYQFMAKDNVPFHGLVFPCSVLGAEDNYTLVKHIIATEYLNYEDGKFSKSRGIGVFGDMAKDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLIKNNSELLNNLGNFINRAGMFVSKFFGGCVPEMALTPDDRRLVAHVSWELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGGEMDRQRAGTVTGMAVNMAALLSVMLQPYMPTVSSTIQTQLQLPPAACRILATSFICTLPAGHRIGTVSPLFQKLENDQIENLRQRFGGGQLEESLELKAKGSPKPAAVEAVTAAGSQHIQTLTDEVTKQGNVVRELKAQKADKNQVAAEVAKLLDLKKQLALAEGKPIETPKGKKKK

Target ORF information:

RefSeq Version	XM_006513568.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus methionine-tRNA synthetase (Mars), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513568.3

ORF Insert Sequence:

ATGGAGGAGG GCCTAACCCC ACGGGAAATC TGTGACAAGT ACCATGCCAT CCATGCTGAC 
ATCTACCGCT GGTTCGGCAT ATCGTTCGAT ACTTTCGGGC GCACTACCAC TCCTCAGCAG 
ACCAAAATCA CCCAGGACAT CTTCCAGAGG TTGCTGACCC GGGGGTTTGT GCTGCGAGAT 
ACTGTGGAGC AGCTTCGGTG TGAGCGGTGT GCACGTTTCC TGGCTGACCG CTTTGTGGAG 
GGTGTGTGTC CCTTCTGTGG CTATGAAGAG GCCCGAGGTG ACCAGTGTGA CAGGTGTGGC 
AAGCTCATCA ATGCCATTGA GCTCAAGAAA CCACAGTGCA AAATCTGCCG CTCCTGCCCT 
GTGGTGAGGT CCTCACAGCA CCTGTTTCTA GACTTGCCTA AGTTGGAAAA GCGTCTGGAG 
GACTGGTTGG GGAAGACAGT GCCTGGCAGT GACTGGACAC CCAATGCCAG GTTCATTATA 
CGTTCCTGGC TTCGAGATGG CCTCAAGCCA CGATGCATCA CCAGAGACCT CAAATGGGGA 
ACGCCTGTGC CCTTGGAAGG TTTTGAGGAC AAGGTATTTT ACGTCTGGTT TGATGCTACT 
ATTGGCTACG TGTCCATCAC AGCCAACTAC ACAGACCAAT GGGAGAAATG GTGGAAGAAC 
CCAGAACAAG TGGACCTTTA CCAGTTCATG GCCAAAGACA ATGTTCCCTT CCATGGCTTG 
GTCTTTCCGT GTTCAGTCCT AGGAGCTGAG GACAACTACA CCCTGGTCAA GCACATCATT 
GCTACAGAGT ACCTGAACTA TGAGGATGGG AAATTCTCTA AGAGCCGGGG CATAGGAGTG 
TTTGGAGACA TGGCCAAGGA TACAGGAATC CCTGCTGACA TCTGGCGATT CTATCTGCTA 
TACATTCGGC CTGAGGGCCA GGACAGTGCC TTCTCCTGGA CAGACTTGTT GATTAAAAAC 
AATTCTGAGC TGCTCAACAA CCTGGGCAAC TTCATCAACA GAGCTGGCAT GTTTGTTTCT 
AAGTTTTTTG GCGGTTGTGT GCCTGAGATG GCGCTAACCC CTGATGACAG ACGCCTGGTG 
GCCCATGTCT CTTGGGAACT CCAGCACTAT CACCAGCTGT TGGAAAAGGT TCGGATCCGG 
GATGCCTTGC GCAGTATCCT CACCATATCT CGCCATGGCA ACCAATACAT TCAAGTGAAT 
GAGCCCTGGA AACGGATTAA AGGTGGTGAG ATGGACAGGC AGCGGGCAGG CACAGTGACA 
GGCATGGCAG TGAACATGGC TGCCTTGCTG TCTGTCATGC TGCAGCCATA CATGCCCACA 
GTCAGCTCTA CCATCCAGAC CCAGCTGCAG CTCCCACCTG CAGCCTGCCG CATCCTTGCC 
ACAAGCTTCA TTTGTACCTT GCCAGCAGGC CACCGAATTG GCACAGTCAG TCCTTTGTTC 
CAAAAACTGG AAAATGACCA GATTGAAAAT TTGAGGCAGC GCTTTGGAGG GGGTCAGCTA 
GAAGAGTCCT TGGAGTTAAA GGCTAAAGGC TCCCCCAAGC CAGCAGCTGT GGAGGCAGTT 
ACAGCAGCAG GCTCGCAGCA CATACAAACG CTGACGGATG AGGTGACCAA GCAGGGCAAC 
GTCGTCCGGG AACTGAAAGC ACAGAAGGCA GACAAGAACC AGGTTGCTGC AGAGGTGGCT 
AAACTCTTGG ATCTAAAGAA ACAGTTGGCT TTGGCTGAGG GGAAACCCAT TGAAACTCCT 
AAAGGCAAGA AGAAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mars1 ( NM_001003913.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001003913.1 Mus musculus methionine-tRNA synthetase 1 (Mars1), transcript variant 2, mRNA.

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CloneID	OMu10532
Clone ID Related Accession (Same CDS sequence)	NM_001003913.2
Accession Version	NM_001003913.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	methionine--tRNA ligase, cytoplasmic isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC114678.20, BC079643.1 and CJ278192.1. On Jan 21, 2010 this sequence version replaced NM_001003913.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded protein (isoform 2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC079643.1, SRR1660821.32652.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGACTGT TCGTGAGCGA GGGTTCCCCG GGGAGCCTGC CCGTGCTGGC TGCGGCCGCG 
AGGGCCCGGG GTCGGGCGGA GCTGCTCATC AGCACCGTAG GCCCCGAAGA GTGTGTGGTA 
CCATTCCTTA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCTGCTAGTG CAATCTGCCG ATATTTTTTT CTGTTATGTG GCTGGGAACA AGATGATCTC 
ACCAACCAGT GGCTGGAATG GGAGGCAACA GAACTGCAGC CAGTTCTGTC TGCTGCCCTA 
CACTGTCTAG TGGTTCAAGG CAAGAAAGGG GAAGATATAC TTGGCCCACT TCGGAGAGTC 
CTGACTCACA TTGATCACAG CTTGAGTCGT CAAAACTGTC CTTTCCTGGC TGGGGACACA 
GAATCTCTAG CTGACATTGT TTTGTGGGGA GCACTGTATC CTTTACTGCA AGACCCAGCT 
TACCTCCCTG AGGAGTTGGG TGCCCTGCAA AGTTGGTTCC AGACACTGAG TACCCAGGAA 
CCGTGTCAGC GAGCTGCAGA GACGGTGCTA AAACAGCAGG GTGTCCTGGC ACTTCGTCTG 
TACCTCCAGA AACAGCCACA GCCTCAGCCC CCGCCTCCTG AGGGGAGAAC TGTCAGCAAC 
GAGCTGGAGG AAGAGGAACT GGCTACCTTG TCTGAGGAGG ACATCGTTAC AGCTGTTGCC 
GCGTGGGAGA AGGGTCTGGA AAGCCTGCCT CCGCTAAAGC TCCAGCAGCA TCCAGTGTTG 
CCTGTGCCTG GAGAGAGGAA TGTTCTCATC ACCAGTGCCC TCCCCTATGT CAACAATGTC 
CCCCACCTTG GAAACATCAT TGGCTGTGTG CTCAGTGCTG ATGTCTTTGC AAGGTATTGT 
CGCCTTCGCC AGTGGAATAC CCTCTATCTG TGTGGTACAG ATGAGTATGG TACTGCGACA 
GAGACCAAGG CCATGGAGGA GGGCCTAACC CCACGGGAAA TCTGTGACAA GTACCATGCC 
ATCCATGCTG ACATCTACCG CTGGTTCGGC ATATCGTTCG ATACTTTCGG GCGCACTACC 
ACTCCTCAGC AGACCAAAAT CACCCAGGAC ATCTTCCAGA GGTTGCTGAC CCGGGGGTTT 
GTGCTGCGAG ATACTGTGGA GCAGCTTCGG TGTGAGCGGT GTGCACGTTT CCTGGCTGAC 
CGCTTTGTGG AGGGTGTGTG TCCCTTCTGT GGCTATGAAG AGGCCCGAGG TGACCAGTGT 
GACAGGTGTG GCAAGCTCAT CAATGCCATT GAGCTCAAGA AACCACAGTG CAAAATCTGC 
CGCTCCTGCC CTGTGGTGAG GTCCTCACAG CACCTGTTTC TAGACTTGCC TAAGTTGGAA 
AAGCGTCTGG AGGACTGGTT GGGGAAGACA GTGCCTGGCA GTGACTGGAC ACCCAATGCC 
AGGTTCATTA TACGTTCCTG GCTTCGAGAT GGCCTCAAGC CACGATGCAT CACCAGAGAC 
CTCAAATGGG GAACGCCTGT GCCCTTGGAA GGTTTTGAGG ACAAGGTATT TTACGTCTGG 
TTTGATGCTA CTATTGGCTA CGTGTCCATC ACAGCCAACT ACACAGACCA ATGGGAGAAA 
TGGTGGAAGA ACCCAGAACA AGTGGACCTT TACCAGTTCA TGGCCAAAGA CAATGTTCCC 
TTCCATGGCT TGGTCTTTCC GTGTTCAGTC CTAGGAGCTG AGGACAACTA CACCCTGGTC 
AAGCACATCA TTGCTACAGA GTACCTGAAC TATGAGGATG GGAAATTCTC TAAGAGCCGG 
GGCATAGGAG TGTTTGGAGA CATGGCCAAG GATACAGGAA TCCCTGCTGA CATCTGGCGA 
TTCTATCTGC TATACATTCG GCCTGAGGGC CAGGACAGTG CCTTCTCCTG GACAGACTTG 
TTGATTAAAA ACAATTCTGA GCTGCTCAAC AACCTGGGCA ACTTCATCAA CAGAGCTGGC 
ATGTTTGTTT CTAAGTTTTT TGGCGGTTGT GTGCCTGAGA TGGCGCTAAC CCCTGATGAC 
AGACGCCTGG TGGCCCATGT CTCTTGGGAA CTCCAGCACT ATCACCAGCT GTTGGAAAAG 
GTTCGGATCC GGGATGCCTT GCGCAGTATC CTCACCATAT CTCGCCATGG CAACCAATAC 
ATTCAAGTGA ATGAGCCCTG GAAACGGATT AAAGGTGGTG AGATGGACAG GCAGCGGGCA 
GGCACAGTGA CAGGCATGGC AGTGAACATG GCTGCCTTGC TGTCTGTCAT GCTGCAGCCA 
TACATGCCCA CAGTCAGCTC TACCATCCAG ACCCAGCTGC AGCTCCCACC TGCAGCCTGC 
CGCATCCTTG CCACAAGCTT CATTTGTACC TTGCCAGCAG GCCACCGAAT TGGCACAGTC 
AGTCCTTTGT TCCAAAAACT GGAAAATGAC CAGATTGAAA ATTTGAGGCA GCGCTTTGGA 
GGGGGTCAGG CTAAAGGCTC CCCCAAGCCA GCAGCTGTGG AGGCAGTTAC AGCAGCAGGC 
TCGCAGCACA TACAAACGCT GACGGATGAG GTGACCAAGC AGGGCAACGT CGTCCGGGAA 
CTGAAAGCAC AGAAGGCAGA CAAGAACCAG GTTGCTGCAG AGGTGGCTAA ACTCTTGGAT 
CTAAAGAAAC AGTTGGCTTT GGCTGAGGGG AAACCCATTG AAACTCCTAA AGGCAAGAAG 
AAAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003913.1
CDS	158..2866
Translation	MRLFVSEGSPGSLPVLAAAARARGRAELLISTVGPEECVVPFLTRPKVPVLQLDSGNYLFSASAICRYFFLLCGWEQDDLTNQWLEWEATELQPVLSAALHCLVVQGKKGEDILGPLRRVLTHIDHSLSRQNCPFLAGDTESLADIVLWGALYPLLQDPAYLPEELGALQSWFQTLSTQEPCQRAAETVLKQQGVLALRLYLQKQPQPQPPPPEGRTVSNELEEEELATLSEEDIVTAVAAWEKGLESLPPLKLQQHPVLPVPGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRQWNTLYLCGTDEYGTATETKAMEEGLTPREICDKYHAIHADIYRWFGISFDTFGRTTTPQQTKITQDIFQRLLTRGFVLRDTVEQLRCERCARFLADRFVEGVCPFCGYEEARGDQCDRCGKLINAIELKKPQCKICRSCPVVRSSQHLFLDLPKLEKRLEDWLGKTVPGSDWTPNARFIIRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYVSITANYTDQWEKWWKNPEQVDLYQFMAKDNVPFHGLVFPCSVLGAEDNYTLVKHIIATEYLNYEDGKFSKSRGIGVFGDMAKDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLIKNNSELLNNLGNFINRAGMFVSKFFGGCVPEMALTPDDRRLVAHVSWELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGGEMDRQRAGTVTGMAVNMAALLSVMLQPYMPTVSSTIQTQLQLPPAACRILATSFICTLPAGHRIGTVSPLFQKLENDQIENLRQRFGGGQAKGSPKPAAVEAVTAAGSQHIQTLTDEVTKQGNVVRELKAQKADKNQVAAEVAKLLDLKKQLALAEGKPIETPKGKKKK

Target ORF information:

RefSeq Version	NM_001003913.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus methionine-tRNA synthetase 1 (Mars1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003913.2

ORF Insert Sequence:

ATGAGACTGT TCGTGAGCGA GGGTTCCCCG GGGAGCCTGC CCGTGCTGGC TGCGGCCGCG 
AGGGCCCGGG GTCGGGCGGA GCTGCTCATC AGCACCGTAG GCCCCGAAGA GTGTGTGGTA 
CCATTCCTTA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCTGCTAGTG CAATCTGCCG ATATTTTTTT CTGTTATGTG GCTGGGAACA AGATGATCTC 
ACCAACCAGT GGCTGGAATG GGAGGCAACA GAACTGCAGC CAGTTCTGTC TGCTGCCCTA 
CACTGTCTAG TGGTTCAAGG CAAGAAAGGG GAAGATATAC TTGGCCCACT TCGGAGAGTC 
CTGACTCACA TTGATCACAG CTTGAGTCGT CAAAACTGTC CTTTCCTGGC TGGGGACACA 
GAATCTCTAG CTGACATTGT TTTGTGGGGA GCACTGTATC CTTTACTGCA AGACCCAGCT 
TACCTCCCTG AGGAGTTGGG TGCCCTGCAA AGTTGGTTCC AGACACTGAG TACCCAGGAA 
CCGTGTCAGC GAGCTGCAGA GACGGTGCTA AAACAGCAGG GTGTCCTGGC ACTTCGTCTG 
TACCTCCAGA AACAGCCACA GCCTCAGCCC CCGCCTCCTG AGGGGAGAAC TGTCAGCAAC 
GAGCTGGAGG AAGAGGAACT GGCTACCTTG TCTGAGGAGG ACATCGTTAC AGCTGTTGCC 
GCGTGGGAGA AGGGTCTGGA AAGCCTGCCT CCGCTAAAGC TCCAGCAGCA TCCAGTGTTG 
CCTGTGCCTG GAGAGAGGAA TGTTCTCATC ACCAGTGCCC TCCCCTATGT CAACAATGTC 
CCCCACCTTG GAAACATCAT TGGCTGTGTG CTCAGTGCTG ATGTCTTTGC AAGGTATTGT 
CGCCTTCGCC AGTGGAATAC CCTCTATCTG TGTGGTACAG ATGAGTATGG TACTGCGACA 
GAGACCAAGG CCATGGAGGA GGGCCTAACC CCACGGGAAA TCTGTGACAA GTACCATGCC 
ATCCATGCTG ACATCTACCG CTGGTTCGGC ATATCGTTCG ATACTTTCGG GCGCACTACC 
ACTCCTCAGC AGACCAAAAT CACCCAGGAC ATCTTCCAGA GGTTGCTGAC CCGGGGGTTT 
GTGCTGCGAG ATACTGTGGA GCAGCTTCGG TGTGAGCGGT GTGCACGTTT CCTGGCTGAC 
CGCTTTGTGG AGGGTGTGTG TCCCTTCTGT GGCTATGAAG AGGCCCGAGG TGACCAGTGT 
GACAGGTGTG GCAAGCTCAT CAATGCCATT GAGCTCAAGA AACCACAGTG CAAAATCTGC 
CGCTCCTGCC CTGTGGTGAG GTCCTCACAG CACCTGTTTC TAGACTTGCC TAAGTTGGAA 
AAGCGTCTGG AGGACTGGTT GGGGAAGACA GTGCCTGGCA GTGACTGGAC ACCCAATGCC 
AGGTTCATTA TACGTTCCTG GCTTCGAGAT GGCCTCAAGC CACGATGCAT CACCAGAGAC 
CTCAAATGGG GAACGCCTGT GCCCTTGGAA GGTTTTGAGG ACAAGGTATT TTACGTCTGG 
TTTGATGCTA CTATTGGCTA CGTGTCCATC ACAGCCAACT ACACAGACCA ATGGGAGAAA 
TGGTGGAAGA ACCCAGAACA AGTGGACCTT TACCAGTTCA TGGCCAAAGA CAATGTTCCC 
TTCCATGGCT TGGTCTTTCC GTGTTCAGTC CTAGGAGCTG AGGACAACTA CACCCTGGTC 
AAGCACATCA TTGCTACAGA GTACCTGAAC TATGAGGATG GGAAATTCTC TAAGAGCCGG 
GGCATAGGAG TGTTTGGAGA CATGGCCAAG GATACAGGAA TCCCTGCTGA CATCTGGCGA 
TTCTATCTGC TATACATTCG GCCTGAGGGC CAGGACAGTG CCTTCTCCTG GACAGACTTG 
TTGATTAAAA ACAATTCTGA GCTGCTCAAC AACCTGGGCA ACTTCATCAA CAGAGCTGGC 
ATGTTTGTTT CTAAGTTTTT TGGCGGTTGT GTGCCTGAGA TGGCGCTAAC CCCTGATGAC 
AGACGCCTGG TGGCCCATGT CTCTTGGGAA CTCCAGCACT ATCACCAGCT GTTGGAAAAG 
GTTCGGATCC GGGATGCCTT GCGCAGTATC CTCACCATAT CTCGCCATGG CAACCAATAC 
ATTCAAGTGA ATGAGCCCTG GAAACGGATT AAAGGTGGTG AGATGGACAG GCAGCGGGCA 
GGCACAGTGA CAGGCATGGC AGTGAACATG GCTGCCTTGC TGTCTGTCAT GCTGCAGCCA 
TACATGCCCA CAGTCAGCTC TACCATCCAG ACCCAGCTGC AGCTCCCACC TGCAGCCTGC 
CGCATCCTTG CCACAAGCTT CATTTGTACC TTGCCAGCAG GCCACCGAAT TGGCACAGTC 
AGTCCTTTGT TCCAAAAACT GGAAAATGAC CAGATTGAAA ATTTGAGGCA GCGCTTTGGA 
GGGGGTCAGG CTAAAGGCTC CCCCAAGCCA GCAGCTGTGG AGGCAGTTAC AGCAGCAGGC 
TCGCAGCACA TACAAACGCT GACGGATGAG GTGACCAAGC AGGGCAACGT CGTCCGGGAA 
CTGAAAGCAC AGAAGGCAGA CAAGAACCAG GTTGCTGCAG AGGTGGCTAA ACTCTTGGAT 
CTAAAGAAAC AGTTGGCTTT GGCTGAGGGG AAACCCATTG AAACTCCTAA AGGCAAGAAG 
AAAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mars1 ( XM_006513568.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513631.1 Mus musculus methionine-tRNA synthetase (Mars), transcript variant X1, mRNA.

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CloneID	OMu44448
Clone ID Related Accession (Same CDS sequence)	XM_006513568.4 , XM_006513568.3
Accession Version	XM_006513568.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	methionine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513568.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GCCTAACCCC ACGGGAAATC TGTGACAAGT ACCATGCCAT CCATGCTGAC 
ATCTACCGCT GGTTCGGCAT ATCGTTCGAT ACTTTCGGGC GCACTACCAC TCCTCAGCAG 
ACCAAAATCA CCCAGGACAT CTTCCAGAGG TTGCTGACCC GGGGGTTTGT GCTGCGAGAT 
ACTGTGGAGC AGCTTCGGTG TGAGCGGTGT GCACGTTTCC TGGCTGACCG CTTTGTGGAG 
GGTGTGTGTC CCTTCTGTGG CTATGAAGAG GCCCGAGGTG ACCAGTGTGA CAGGTGTGGC 
AAGCTCATCA ATGCCATTGA GCTCAAGAAA CCACAGTGCA AAATCTGCCG CTCCTGCCCT 
GTGGTGAGGT CCTCACAGCA CCTGTTTCTA GACTTGCCTA AGTTGGAAAA GCGTCTGGAG 
GACTGGTTGG GGAAGACAGT GCCTGGCAGT GACTGGACAC CCAATGCCAG GTTCATTATA 
CGTTCCTGGC TTCGAGATGG CCTCAAGCCA CGATGCATCA CCAGAGACCT CAAATGGGGA 
ACGCCTGTGC CCTTGGAAGG TTTTGAGGAC AAGGTATTTT ACGTCTGGTT TGATGCTACT 
ATTGGCTACG TGTCCATCAC AGCCAACTAC ACAGACCAAT GGGAGAAATG GTGGAAGAAC 
CCAGAACAAG TGGACCTTTA CCAGTTCATG GCCAAAGACA ATGTTCCCTT CCATGGCTTG 
GTCTTTCCGT GTTCAGTCCT AGGAGCTGAG GACAACTACA CCCTGGTCAA GCACATCATT 
GCTACAGAGT ACCTGAACTA TGAGGATGGG AAATTCTCTA AGAGCCGGGG CATAGGAGTG 
TTTGGAGACA TGGCCAAGGA TACAGGAATC CCTGCTGACA TCTGGCGATT CTATCTGCTA 
TACATTCGGC CTGAGGGCCA GGACAGTGCC TTCTCCTGGA CAGACTTGTT GATTAAAAAC 
AATTCTGAGC TGCTCAACAA CCTGGGCAAC TTCATCAACA GAGCTGGCAT GTTTGTTTCT 
AAGTTTTTTG GCGGTTGTGT GCCTGAGATG GCGCTAACCC CTGATGACAG ACGCCTGGTG 
GCCCATGTCT CTTGGGAACT CCAGCACTAT CACCAGCTGT TGGAAAAGGT TCGGATCCGG 
GATGCCTTGC GCAGTATCCT CACCATATCT CGCCATGGCA ACCAATACAT TCAAGTGAAT 
GAGCCCTGGA AACGGATTAA AGGTGGTGAG ATGGACAGGC AGCGGGCAGG CACAGTGACA 
GGCATGGCAG TGAACATGGC TGCCTTGCTG TCTGTCATGC TGCAGCCATA CATGCCCACA 
GTCAGCTCTA CCATCCAGAC CCAGCTGCAG CTCCCACCTG CAGCCTGCCG CATCCTTGCC 
ACAAGCTTCA TTTGTACCTT GCCAGCAGGC CACCGAATTG GCACAGTCAG TCCTTTGTTC 
CAAAAACTGG AAAATGACCA GATTGAAAAT TTGAGGCAGC GCTTTGGAGG GGGTCAGCTA 
GAAGAGTCCT TGGAGTTAAA GGCTAAAGGC TCCCCCAAGC CAGCAGCTGT GGAGGCAGTT 
ACAGCAGCAG GCTCGCAGCA CATACAAACG CTGACGGATG AGGTGACCAA GCAGGGCAAC 
GTCGTCCGGG AACTGAAAGC ACAGAAGGCA GACAAGAACC AGGTTGCTGC AGAGGTGGCT 
AAACTCTTGG ATCTAAAGAA ACAGTTGGCT TTGGCTGAGG GGAAACCCAT TGAAACTCCT 
AAAGGCAAGA AGAAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513631.1
CDS	2683..4443
Translation	MEEGLTPREICDKYHAIHADIYRWFGISFDTFGRTTTPQQTKITQDIFQRLLTRGFVLRDTVEQLRCERCARFLADRFVEGVCPFCGYEEARGDQCDRCGKLINAIELKKPQCKICRSCPVVRSSQHLFLDLPKLEKRLEDWLGKTVPGSDWTPNARFIIRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYVSITANYTDQWEKWWKNPEQVDLYQFMAKDNVPFHGLVFPCSVLGAEDNYTLVKHIIATEYLNYEDGKFSKSRGIGVFGDMAKDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLIKNNSELLNNLGNFINRAGMFVSKFFGGCVPEMALTPDDRRLVAHVSWELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGGEMDRQRAGTVTGMAVNMAALLSVMLQPYMPTVSSTIQTQLQLPPAACRILATSFICTLPAGHRIGTVSPLFQKLENDQIENLRQRFGGGQLEESLELKAKGSPKPAAVEAVTAAGSQHIQTLTDEVTKQGNVVRELKAQKADKNQVAAEVAKLLDLKKQLALAEGKPIETPKGKKKK

Target ORF information:

RefSeq Version	XM_006513568.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus methionine-tRNA synthetase (Mars), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513568.4

ORF Insert Sequence:

ATGGAGGAGG GCCTAACCCC ACGGGAAATC TGTGACAAGT ACCATGCCAT CCATGCTGAC 
ATCTACCGCT GGTTCGGCAT ATCGTTCGAT ACTTTCGGGC GCACTACCAC TCCTCAGCAG 
ACCAAAATCA CCCAGGACAT CTTCCAGAGG TTGCTGACCC GGGGGTTTGT GCTGCGAGAT 
ACTGTGGAGC AGCTTCGGTG TGAGCGGTGT GCACGTTTCC TGGCTGACCG CTTTGTGGAG 
GGTGTGTGTC CCTTCTGTGG CTATGAAGAG GCCCGAGGTG ACCAGTGTGA CAGGTGTGGC 
AAGCTCATCA ATGCCATTGA GCTCAAGAAA CCACAGTGCA AAATCTGCCG CTCCTGCCCT 
GTGGTGAGGT CCTCACAGCA CCTGTTTCTA GACTTGCCTA AGTTGGAAAA GCGTCTGGAG 
GACTGGTTGG GGAAGACAGT GCCTGGCAGT GACTGGACAC CCAATGCCAG GTTCATTATA 
CGTTCCTGGC TTCGAGATGG CCTCAAGCCA CGATGCATCA CCAGAGACCT CAAATGGGGA 
ACGCCTGTGC CCTTGGAAGG TTTTGAGGAC AAGGTATTTT ACGTCTGGTT TGATGCTACT 
ATTGGCTACG TGTCCATCAC AGCCAACTAC ACAGACCAAT GGGAGAAATG GTGGAAGAAC 
CCAGAACAAG TGGACCTTTA CCAGTTCATG GCCAAAGACA ATGTTCCCTT CCATGGCTTG 
GTCTTTCCGT GTTCAGTCCT AGGAGCTGAG GACAACTACA CCCTGGTCAA GCACATCATT 
GCTACAGAGT ACCTGAACTA TGAGGATGGG AAATTCTCTA AGAGCCGGGG CATAGGAGTG 
TTTGGAGACA TGGCCAAGGA TACAGGAATC CCTGCTGACA TCTGGCGATT CTATCTGCTA 
TACATTCGGC CTGAGGGCCA GGACAGTGCC TTCTCCTGGA CAGACTTGTT GATTAAAAAC 
AATTCTGAGC TGCTCAACAA CCTGGGCAAC TTCATCAACA GAGCTGGCAT GTTTGTTTCT 
AAGTTTTTTG GCGGTTGTGT GCCTGAGATG GCGCTAACCC CTGATGACAG ACGCCTGGTG 
GCCCATGTCT CTTGGGAACT CCAGCACTAT CACCAGCTGT TGGAAAAGGT TCGGATCCGG 
GATGCCTTGC GCAGTATCCT CACCATATCT CGCCATGGCA ACCAATACAT TCAAGTGAAT 
GAGCCCTGGA AACGGATTAA AGGTGGTGAG ATGGACAGGC AGCGGGCAGG CACAGTGACA 
GGCATGGCAG TGAACATGGC TGCCTTGCTG TCTGTCATGC TGCAGCCATA CATGCCCACA 
GTCAGCTCTA CCATCCAGAC CCAGCTGCAG CTCCCACCTG CAGCCTGCCG CATCCTTGCC 
ACAAGCTTCA TTTGTACCTT GCCAGCAGGC CACCGAATTG GCACAGTCAG TCCTTTGTTC 
CAAAAACTGG AAAATGACCA GATTGAAAAT TTGAGGCAGC GCTTTGGAGG GGGTCAGCTA 
GAAGAGTCCT TGGAGTTAAA GGCTAAAGGC TCCCCCAAGC CAGCAGCTGT GGAGGCAGTT 
ACAGCAGCAG GCTCGCAGCA CATACAAACG CTGACGGATG AGGTGACCAA GCAGGGCAAC 
GTCGTCCGGG AACTGAAAGC ACAGAAGGCA GACAAGAACC AGGTTGCTGC AGAGGTGGCT 
AAACTCTTGG ATCTAAAGAA ACAGTTGGCT TTGGCTGAGG GGAAACCCAT TGAAACTCCT 
AAAGGCAAGA AGAAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mars1 ( NM_001171582.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001165053.1 Mus musculus methionine-tRNA synthetase 1 (Mars1), transcript variant 1, mRNA.

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CloneID	OMu11847
Clone ID Related Accession (Same CDS sequence)	NM_001171582.1
Accession Version	NM_001171582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	methionine--tRNA ligase, cytoplasmic isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC114678.20, BC079643.1, AA510607.1 and CJ278192.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGACTGT TCGTGAGCGA GGGTTCCCCG GGGAGCCTGC CCGTGCTGGC TGCGGCCGCG 
AGGGCCCGGG GTCGGGCGGA GCTGCTCATC AGCACCGTAG GCCCCGAAGA GTGTGTGGTA 
CCATTCCTTA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCTGCTAGTG CAATCTGCCG ATATTTTTTT CTGTTATGTG GCTGGGAACA AGATGATCTC 
ACCAACCAGT GGCTGGAATG GGAGGCAACA GAACTGCAGC CAGTTCTGTC TGCTGCCCTA 
CACTGTCTAG TGGTTCAAGG CAAGAAAGGG GAAGATATAC TTGGCCCACT TCGGAGAGTC 
CTGACTCACA TTGATCACAG CTTGAGTCGT CAAAACTGTC CTTTCCTGGC TGGGGACACA 
GAATCTCTAG CTGACATTGT TTTGTGGGGA GCACTGTATC CTTTACTGCA AGACCCAGCT 
TACCTCCCTG AGGAGTTGGG TGCCCTGCAA AGTTGGTTCC AGACACTGAG TACCCAGGAA 
CCGTGTCAGC GAGCTGCAGA GACGGTGCTA AAACAGCAGG GTGTCCTGGC ACTTCGTCTG 
TACCTCCAGA AACAGCCACA GCCTCAGCCC CCGCCTCCTG AGGGGAGAAC TGTCAGCAAC 
GAGCTGGAGG AAGAGGAACT GGCTACCTTG TCTGAGGAGG ACATCGTTAC AGCTGTTGCC 
GCGTGGGAGA AGGGTCTGGA AAGCCTGCCT CCGCTAAAGC TCCAGCAGCA TCCAGTGTTG 
CCTGTGCCTG GAGAGAGGAA TGTTCTCATC ACCAGTGCCC TCCCCTATGT CAACAATGTC 
CCCCACCTTG GAAACATCAT TGGCTGTGTG CTCAGTGCTG ATGTCTTTGC AAGGTATTGT 
CGCCTTCGCC AGTGGAATAC CCTCTATCTG TGTGGTACAG ATGAGTATGG TACTGCGACA 
GAGACCAAGG CCATGGAGGA GGGCCTAACC CCACGGGAAA TCTGTGACAA GTACCATGCC 
ATCCATGCTG ACATCTACCG CTGGTTCGGC ATATCGTTCG ATACTTTCGG GCGCACTACC 
ACTCCTCAGC AGACCAAAAT CACCCAGGAC ATCTTCCAGA GGTTGCTGAC CCGGGGGTTT 
GTGCTGCGAG ATACTGTGGA GCAGCTTCGG TGTGAGCGGT GTGCACGTTT CCTGGCTGAC 
CGCTTTGTGG AGGGTGTGTG TCCCTTCTGT GGCTATGAAG AGGCCCGAGG TGACCAGTGT 
GACAGGTGTG GCAAGCTCAT CAATGCCATT GAGCTCAAGA AACCACAGTG CAAAATCTGC 
CGCTCCTGCC CTGTGGTGAG GTCCTCACAG CACCTGTTTC TAGACTTGCC TAAGTTGGAA 
AAGCGTCTGG AGGACTGGTT GGGGAAGACA GTGCCTGGCA GTGACTGGAC ACCCAATGCC 
AGGTTCATTA TACGTTCCTG GCTTCGAGAT GGCCTCAAGC CACGATGCAT CACCAGAGAC 
CTCAAATGGG GAACGCCTGT GCCCTTGGAA GGTTTTGAGG ACAAGGTATT TTACGTCTGG 
TTTGATGCTA CTATTGGCTA CGTGTCCATC ACAGCCAACT ACACAGACCA ATGGGAGAAA 
TGGTGGAAGA ACCCAGAACA AGTGGACCTT TACCAGTTCA TGGCCAAAGA CAATGTTCCC 
TTCCATGGCT TGGTCTTTCC GTGTTCAGTC CTAGGAGCTG AGGACAACTA CACCCTGGTC 
AAGCACATCA TTGCTACAGA GTACCTGAAC TATGAGGATG GGAAATTCTC TAAGAGCCGG 
GGCATAGGAG TGTTTGGAGA CATGGCCAAG GATACAGGAA TCCCTGCTGA CATCTGGCGA 
TTCTATCTGC TATACATTCG GCCTGAGGGC CAGGACAGTG CCTTCTCCTG GACAGACTTG 
TTGATTAAAA ACAATTCTGA GCTGCTCAAC AACCTGGGCA ACTTCATCAA CAGAGCTGGC 
ATGTTTGTTT CTAAGTTTTT TGGCGGTTGT GTGCCTGAGA TGGCGCTAAC CCCTGATGAC 
AGACGCCTGG TGGCCCATGT CTCTTGGGAA CTCCAGCACT ATCACCAGCT GTTGGAAAAG 
GTTCGGATCC GGGATGCCTT GCGCAGTATC CTCACCATAT CTCGCCATGG CAACCAATAC 
ATTCAAGTGA ATGAGCCCTG GAAACGGATT AAAGGTGGTG AGATGGACAG GCAGCGGGCA 
GGCACAGTGA CAGGCATGGC AGTGAACATG GCTGCCTTGC TGTCTGTCAT GCTGCAGCCA 
TACATGCCCA CAGTCAGCTC TACCATCCAG ACCCAGCTGC AGCTCCCACC TGCAGCCTGC 
CGCATCCTTG CCACAAGCTT CATTTGTACC TTGCCAGCAG GCCACCGAAT TGGCACAGTC 
AGTCCTTTGT TCCAAAAACT GGAAAATGAC CAGATTGAAA ATTTGAGGCA GCGCTTTGGA 
GGGGGTCAGC TAGAAGAGTC CTTGGAGTTA AAGGCTAAAG GCTCCCCCAA GCCAGCAGCT 
GTGGAGGCAG TTACAGCAGC AGGCTCGCAG CACATACAAA CGCTGACGGA TGAGGTGACC 
AAGCAGGGCA ACGTCGTCCG GGAACTGAAA GCACAGAAGG CAGACAAGAA CCAGGTTGCT 
GCAGAGGTGG CTAAACTCTT GGATCTAAAG AAACAGTTGG CTTTGGCTGA GGGGAAACCC 
ATTGAAACTC CTAAAGGCAA GAAGAAAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165053.1
CDS	158..2890
Translation	MRLFVSEGSPGSLPVLAAAARARGRAELLISTVGPEECVVPFLTRPKVPVLQLDSGNYLFSASAICRYFFLLCGWEQDDLTNQWLEWEATELQPVLSAALHCLVVQGKKGEDILGPLRRVLTHIDHSLSRQNCPFLAGDTESLADIVLWGALYPLLQDPAYLPEELGALQSWFQTLSTQEPCQRAAETVLKQQGVLALRLYLQKQPQPQPPPPEGRTVSNELEEEELATLSEEDIVTAVAAWEKGLESLPPLKLQQHPVLPVPGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRQWNTLYLCGTDEYGTATETKAMEEGLTPREICDKYHAIHADIYRWFGISFDTFGRTTTPQQTKITQDIFQRLLTRGFVLRDTVEQLRCERCARFLADRFVEGVCPFCGYEEARGDQCDRCGKLINAIELKKPQCKICRSCPVVRSSQHLFLDLPKLEKRLEDWLGKTVPGSDWTPNARFIIRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYVSITANYTDQWEKWWKNPEQVDLYQFMAKDNVPFHGLVFPCSVLGAEDNYTLVKHIIATEYLNYEDGKFSKSRGIGVFGDMAKDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLIKNNSELLNNLGNFINRAGMFVSKFFGGCVPEMALTPDDRRLVAHVSWELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGGEMDRQRAGTVTGMAVNMAALLSVMLQPYMPTVSSTIQTQLQLPPAACRILATSFICTLPAGHRIGTVSPLFQKLENDQIENLRQRFGGGQLEESLELKAKGSPKPAAVEAVTAAGSQHIQTLTDEVTKQGNVVRELKAQKADKNQVAAEVAKLLDLKKQLALAEGKPIETPKGKKKK

Target ORF information:

RefSeq Version	NM_001171582.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus methionine-tRNA synthetase 1 (Mars1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171582.1

ORF Insert Sequence:

ATGAGACTGT TCGTGAGCGA GGGTTCCCCG GGGAGCCTGC CCGTGCTGGC TGCGGCCGCG 
AGGGCCCGGG GTCGGGCGGA GCTGCTCATC AGCACCGTAG GCCCCGAAGA GTGTGTGGTA 
CCATTCCTTA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGTGGCAA CTACCTCTTC 
TCTGCTAGTG CAATCTGCCG ATATTTTTTT CTGTTATGTG GCTGGGAACA AGATGATCTC 
ACCAACCAGT GGCTGGAATG GGAGGCAACA GAACTGCAGC CAGTTCTGTC TGCTGCCCTA 
CACTGTCTAG TGGTTCAAGG CAAGAAAGGG GAAGATATAC TTGGCCCACT TCGGAGAGTC 
CTGACTCACA TTGATCACAG CTTGAGTCGT CAAAACTGTC CTTTCCTGGC TGGGGACACA 
GAATCTCTAG CTGACATTGT TTTGTGGGGA GCACTGTATC CTTTACTGCA AGACCCAGCT 
TACCTCCCTG AGGAGTTGGG TGCCCTGCAA AGTTGGTTCC AGACACTGAG TACCCAGGAA 
CCGTGTCAGC GAGCTGCAGA GACGGTGCTA AAACAGCAGG GTGTCCTGGC ACTTCGTCTG 
TACCTCCAGA AACAGCCACA GCCTCAGCCC CCGCCTCCTG AGGGGAGAAC TGTCAGCAAC 
GAGCTGGAGG AAGAGGAACT GGCTACCTTG TCTGAGGAGG ACATCGTTAC AGCTGTTGCC 
GCGTGGGAGA AGGGTCTGGA AAGCCTGCCT CCGCTAAAGC TCCAGCAGCA TCCAGTGTTG 
CCTGTGCCTG GAGAGAGGAA TGTTCTCATC ACCAGTGCCC TCCCCTATGT CAACAATGTC 
CCCCACCTTG GAAACATCAT TGGCTGTGTG CTCAGTGCTG ATGTCTTTGC AAGGTATTGT 
CGCCTTCGCC AGTGGAATAC CCTCTATCTG TGTGGTACAG ATGAGTATGG TACTGCGACA 
GAGACCAAGG CCATGGAGGA GGGCCTAACC CCACGGGAAA TCTGTGACAA GTACCATGCC 
ATCCATGCTG ACATCTACCG CTGGTTCGGC ATATCGTTCG ATACTTTCGG GCGCACTACC 
ACTCCTCAGC AGACCAAAAT CACCCAGGAC ATCTTCCAGA GGTTGCTGAC CCGGGGGTTT 
GTGCTGCGAG ATACTGTGGA GCAGCTTCGG TGTGAGCGGT GTGCACGTTT CCTGGCTGAC 
CGCTTTGTGG AGGGTGTGTG TCCCTTCTGT GGCTATGAAG AGGCCCGAGG TGACCAGTGT 
GACAGGTGTG GCAAGCTCAT CAATGCCATT GAGCTCAAGA AACCACAGTG CAAAATCTGC 
CGCTCCTGCC CTGTGGTGAG GTCCTCACAG CACCTGTTTC TAGACTTGCC TAAGTTGGAA 
AAGCGTCTGG AGGACTGGTT GGGGAAGACA GTGCCTGGCA GTGACTGGAC ACCCAATGCC 
AGGTTCATTA TACGTTCCTG GCTTCGAGAT GGCCTCAAGC CACGATGCAT CACCAGAGAC 
CTCAAATGGG GAACGCCTGT GCCCTTGGAA GGTTTTGAGG ACAAGGTATT TTACGTCTGG 
TTTGATGCTA CTATTGGCTA CGTGTCCATC ACAGCCAACT ACACAGACCA ATGGGAGAAA 
TGGTGGAAGA ACCCAGAACA AGTGGACCTT TACCAGTTCA TGGCCAAAGA CAATGTTCCC 
TTCCATGGCT TGGTCTTTCC GTGTTCAGTC CTAGGAGCTG AGGACAACTA CACCCTGGTC 
AAGCACATCA TTGCTACAGA GTACCTGAAC TATGAGGATG GGAAATTCTC TAAGAGCCGG 
GGCATAGGAG TGTTTGGAGA CATGGCCAAG GATACAGGAA TCCCTGCTGA CATCTGGCGA 
TTCTATCTGC TATACATTCG GCCTGAGGGC CAGGACAGTG CCTTCTCCTG GACAGACTTG 
TTGATTAAAA ACAATTCTGA GCTGCTCAAC AACCTGGGCA ACTTCATCAA CAGAGCTGGC 
ATGTTTGTTT CTAAGTTTTT TGGCGGTTGT GTGCCTGAGA TGGCGCTAAC CCCTGATGAC 
AGACGCCTGG TGGCCCATGT CTCTTGGGAA CTCCAGCACT ATCACCAGCT GTTGGAAAAG 
GTTCGGATCC GGGATGCCTT GCGCAGTATC CTCACCATAT CTCGCCATGG CAACCAATAC 
ATTCAAGTGA ATGAGCCCTG GAAACGGATT AAAGGTGGTG AGATGGACAG GCAGCGGGCA 
GGCACAGTGA CAGGCATGGC AGTGAACATG GCTGCCTTGC TGTCTGTCAT GCTGCAGCCA 
TACATGCCCA CAGTCAGCTC TACCATCCAG ACCCAGCTGC AGCTCCCACC TGCAGCCTGC 
CGCATCCTTG CCACAAGCTT CATTTGTACC TTGCCAGCAG GCCACCGAAT TGGCACAGTC 
AGTCCTTTGT TCCAAAAACT GGAAAATGAC CAGATTGAAA ATTTGAGGCA GCGCTTTGGA 
GGGGGTCAGC TAGAAGAGTC CTTGGAGTTA AAGGCTAAAG GCTCCCCCAA GCCAGCAGCT 
GTGGAGGCAG TTACAGCAGC AGGCTCGCAG CACATACAAA CGCTGACGGA TGAGGTGACC 
AAGCAGGGCA ACGTCGTCCG GGAACTGAAA GCACAGAAGG CAGACAAGAA CCAGGTTGCT 
GCAGAGGTGG CTAAACTCTT GGATCTAAAG AAACAGTTGG CTTTGGCTGA GGGGAAACCC 
ATTGAAACTC CTAAAGGCAA GAAGAAAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

p38 is essential for the assembly and stability of macromolecular tRNA synthetase complex: implications for its physiological significance.
Proceedings of the National Academy of Sciences of the United States of America99(12)7912-6(2002 Jun)
Kim JY,Kang YS,Lee JW,Kim HJ,Ahn YH,Park H,Ko YG,Kim S

CHOP/GADD153 and methionyl-tRNA synthetase (MetRS) genes overlap in a conserved region that controls mRNA stability.
Biochemical and biophysical research communications262(1)31-8(1999 Aug)
Ubeda M,Schmitt-Ney M,Ferrer J,Habener JF

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Mars1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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