MAN2A2 cDNA ORF clone, Homo sapiens(human)

The following MAN2A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAN2A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu58839 XM_011521565.2
Latest version!
Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu58840 XM_011521566.2
Latest version!
Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu30881 NM_006122.4
Latest version!
Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu48296 NM_001320977.2
Latest version!
Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu30881 XM_024449922.1
Latest version!
Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu30881 XM_024449923.1
Latest version!
Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu30881 XM_017022185.1
Latest version!
Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu30881 XM_024449924.1
Latest version!
Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu48296 XM_005254910.3
Latest version!
Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu48296 XM_024449921.1
Latest version!
Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu48296 XM_024449920.1
Latest version!
Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu48296 XM_017022184.1
Latest version!
Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
View Old Accession Versions >>
OHu48296 NM_001320977.1 Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu30881 NM_006122.3 Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu58839
    Clone ID Related Accession (Same CDS sequence) XM_011521565.2
    Accession Version XM_011521565.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2x isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGACGTCCC CCATACCTGT GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC 
    GCCTGCCTGG TGGGCGCATC AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG
    CCAACGTGGC AGAGAGGGCA GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT
    TCCGAAGCAA CGTCCTCCTG GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG
    AGTGGGATGC CCAGTTCTTC AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA
    ACCTCCATGT GCAGGGGGGC AGAGGTTCTG TACAGCCTGG CTGCAGCTCA CGCTCGCCGC
    TCTGGTCTGG CTGGCCGGTA CCCACTGTCT GATTTCACCC TCCTGACGGA AGCTCGGCGC
    ACATTGGGGC TCTTCCAGCA TCACGATGCC ATCACTGGCA CGGCCAAGGA GGCTGTGGTG
    GTGGACTATG GGGTCAGGCT TCTGCGCTCC CTTGTCAACC TGAAGCAGGT CATCATTCAT
    GCAGCCCACT ATCTGGTGCT GGGGGACAAG GAGACCTACC ACTTTGACCC TGAGGCGCCC
    TTCCTCCAAG TGGATGACAC TCGCTTAAGT CACGACGCCC TCCCAGAGCG CACGGTGATC
    CAGCTGGATT CCTCGCCCAG GTTTGTGGTC CTATTCAACC CACTGGAACA GGAGCGATTC
    AGCATGGTGT CCCTGCTGGT CAACTCTCCC CGCGTGCGTG TCCTTTCGGA GGAGGGTCAG
    CCCCTGGCCG TGCAGATCAG CGCACACTGG AGCTCTGCCA CCGAGGCGGT CCCTGACGTC
    TACCAGGTGT CTGTGCCTGT CCGCCTGCCA GCCCTGGGCC TGGGCGTGCT GCAGCTACAG
    CTGGGCCTGG ATGGGCACCG CACGCTGCCC TCCTCTGTGC GCATCTACCT GCACGGCCGG
    CAGCTGTCCG TCAGCAGGCA CGAAGCGTTT CCTCTCCGTG TCATTGACTC TGGCACCAGC
    GACTTCGCCC TCAGCAACCG CTACATGCAG GTCTGGTTCT CAGGCCTTAC TGGGCTCCTC
    AAGAGCATCC GAAGGGTGGA TGAGGAGCAC GAGCAGCAGG TGGACATGCA GGTCCTTGTC
    TATGGCACCC GTACGTCCAA AGACAAGAGT GGAGCCTACC TCTTCCTGCC CGATGGCGAG
    GCCAAGCCCT ACGTCCCCAA GGAGCCCCCC GTGCTGCGTG TCACTGAAGG CCCTTTCTTC
    TCAGAGGTGG TTGCGTACTA TGAGCACATT CACCAGGCGG TCCGGCTTTA CAATCTGCCA
    GGGGTGGAGG GGCTGTCTCT GGACATATCA TCCCTGGTGG ACATCCGGGA CTACGTCAAC
    AAGGAGCTGG CCCTGCACAT CCATACAGAC ATCGACAGCC AGGGTATCTT CTTCACAGAC
    CTCAATGGCT TTCAGGTGCA GCCCCGACGG TATCTGAAGA AGCTCCCCCT CCAGGCCAAC
    TTCTACCCCA TGCCAGTCAT GGCCTATATC CAGGACGCAC AGAAGCGCCT CACGCTGCAC
    ACTGCCCAGG CCCTGGGTGT CTCTAGCCTC AAAGATGGCC AGCTGGAGGT GATCTTGGAC
    CGGCGGCTGA TGCAGGATGA CAACCGGGGC CTAGGCCAAG GGCTCAAGGA CAACAAGAGA
    ACCTGCAACC GTTTCCGCCT CCTGCTAGAG CGGCGAACCG TGGGCAGTGA GCCTGACTTT
    TTCTCCAAAC TGGCAGCCAT GTTTAGGGGC TTGATCTTTC ACAGCAGCAG GAGCGGTAAC
    CGAGAGGTCC AAGATAGCCA CTCTACCAGC TACCCATCCC TCCTCAGCCA CCTGACCTCC
    ATGTACCTGA ACGCCCCGGC GCTCGCTCTG CCTGTAGCCA GGATGCAGCT CCCAGGCCCT
    GGTCTGCGCT CATTTCATCC TCTGGCTTCC TCACTGCCCT GTGACTTCCA CCTGCTCAAC
    CTACGTACGC TCCAGGCTGA GGAGGACACC CTACCCTCGG CGGAGACCGC ACTCATCTTA
    CACCGCAAGG GTTTTGACTG CGGCCTGGAG GCCAAGAACT TGGGCTTCAA CTGCACCACA
    AGCCAAGGCA AGGTAGCCCT GGGCAGCCTT TTCCATGGCC TGGATGTGGT ATTCCTTCAG
    CCAACCTCCT TGACGTTACT GTACCCTCTG GCCTCCCCGT CCAACAGCAC TGACGTCTAT
    TTGGAGCCCA TGGAGATTGC TACCTTTCGC CTCCGCTTGG GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519867.1
    CDS324..2588
    Translation

    Target ORF information:

    RefSeq Version XM_011521565.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521565.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGACGTCCC CCATACCTGT GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC 
    GCCTGCCTGG TGGGCGCATC AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG
    CCAACGTGGC AGAGAGGGCA GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT
    TCCGAAGCAA CGTCCTCCTG GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG
    AGTGGGATGC CCAGTTCTTC AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA
    ACCTCCATGT GCAGGGGGGC AGAGGTTCTG TACAGCCTGG CTGCAGCTCA CGCTCGCCGC
    TCTGGTCTGG CTGGCCGGTA CCCACTGTCT GATTTCACCC TCCTGACGGA AGCTCGGCGC
    ACATTGGGGC TCTTCCAGCA TCACGATGCC ATCACTGGCA CGGCCAAGGA GGCTGTGGTG
    GTGGACTATG GGGTCAGGCT TCTGCGCTCC CTTGTCAACC TGAAGCAGGT CATCATTCAT
    GCAGCCCACT ATCTGGTGCT GGGGGACAAG GAGACCTACC ACTTTGACCC TGAGGCGCCC
    TTCCTCCAAG TGGATGACAC TCGCTTAAGT CACGACGCCC TCCCAGAGCG CACGGTGATC
    CAGCTGGATT CCTCGCCCAG GTTTGTGGTC CTATTCAACC CACTGGAACA GGAGCGATTC
    AGCATGGTGT CCCTGCTGGT CAACTCTCCC CGCGTGCGTG TCCTTTCGGA GGAGGGTCAG
    CCCCTGGCCG TGCAGATCAG CGCACACTGG AGCTCTGCCA CCGAGGCGGT CCCTGACGTC
    TACCAGGTGT CTGTGCCTGT CCGCCTGCCA GCCCTGGGCC TGGGCGTGCT GCAGCTACAG
    CTGGGCCTGG ATGGGCACCG CACGCTGCCC TCCTCTGTGC GCATCTACCT GCACGGCCGG
    CAGCTGTCCG TCAGCAGGCA CGAAGCGTTT CCTCTCCGTG TCATTGACTC TGGCACCAGC
    GACTTCGCCC TCAGCAACCG CTACATGCAG GTCTGGTTCT CAGGCCTTAC TGGGCTCCTC
    AAGAGCATCC GAAGGGTGGA TGAGGAGCAC GAGCAGCAGG TGGACATGCA GGTCCTTGTC
    TATGGCACCC GTACGTCCAA AGACAAGAGT GGAGCCTACC TCTTCCTGCC CGATGGCGAG
    GCCAAGCCCT ACGTCCCCAA GGAGCCCCCC GTGCTGCGTG TCACTGAAGG CCCTTTCTTC
    TCAGAGGTGG TTGCGTACTA TGAGCACATT CACCAGGCGG TCCGGCTTTA CAATCTGCCA
    GGGGTGGAGG GGCTGTCTCT GGACATATCA TCCCTGGTGG ACATCCGGGA CTACGTCAAC
    AAGGAGCTGG CCCTGCACAT CCATACAGAC ATCGACAGCC AGGGTATCTT CTTCACAGAC
    CTCAATGGCT TTCAGGTGCA GCCCCGACGG TATCTGAAGA AGCTCCCCCT CCAGGCCAAC
    TTCTACCCCA TGCCAGTCAT GGCCTATATC CAGGACGCAC AGAAGCGCCT CACGCTGCAC
    ACTGCCCAGG CCCTGGGTGT CTCTAGCCTC AAAGATGGCC AGCTGGAGGT GATCTTGGAC
    CGGCGGCTGA TGCAGGATGA CAACCGGGGC CTAGGCCAAG GGCTCAAGGA CAACAAGAGA
    ACCTGCAACC GTTTCCGCCT CCTGCTAGAG CGGCGAACCG TGGGCAGTGA GCCTGACTTT
    TTCTCCAAAC TGGCAGCCAT GTTTAGGGGC TTGATCTTTC ACAGCAGCAG GAGCGGTAAC
    CGAGAGGTCC AAGATAGCCA CTCTACCAGC TACCCATCCC TCCTCAGCCA CCTGACCTCC
    ATGTACCTGA ACGCCCCGGC GCTCGCTCTG CCTGTAGCCA GGATGCAGCT CCCAGGCCCT
    GGTCTGCGCT CATTTCATCC TCTGGCTTCC TCACTGCCCT GTGACTTCCA CCTGCTCAAC
    CTACGTACGC TCCAGGCTGA GGAGGACACC CTACCCTCGG CGGAGACCGC ACTCATCTTA
    CACCGCAAGG GTTTTGACTG CGGCCTGGAG GCCAAGAACT TGGGCTTCAA CTGCACCACA
    AGCCAAGGCA AGGTAGCCCT GGGCAGCCTT TTCCATGGCC TGGATGTGGT ATTCCTTCAG
    CCAACCTCCT TGACGTTACT GTACCCTCTG GCCTCCCCGT CCAACAGCAC TGACGTCTAT
    TTGGAGCCCA TGGAGATTGC TACCTTTCGC CTCCGCTTGG GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58840
    Clone ID Related Accession (Same CDS sequence) XM_011521566.2
    Accession Version XM_011521566.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2x isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGACGTCCC CCATACCTGT GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC 
    GCCTGCCTGG TGGGCGCATC AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG
    CCAACGTGGC AGAGAGGGCA GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT
    TCCGAAGCAA CGTCCTCCTG GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG
    AGTGGGATGC CCAGTTCTTC AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA
    ACCTCCATGT GCAGGGGGGC AGAGGTTCTG TACAGCCTGG CTGCAGCTCA CGCTCGCCGC
    TCTGGTCTGG CTGGCCGGTA CCCACTGTCT GATTTCACCC TCCTGACGGA AGCTCGGCGC
    ACATTGGGGC TCTTCCAGCA TCACGATGCC ATCACTGGCA CGGCCAAGGA GGCTGTGGTG
    GTGGACTATG GGGTCAGGCT TCTGCGCTCC CTTGTCAACC TGAAGCAGGT CATCATTCAT
    GCAGCCCACT ATCTGGTGCT GGGGGACAAG GAGACCTACC ACTTTGACCC TGAGGCGCCC
    TTCCTCCAAG TGGATGACAC TCGCTTAAGT CACGACGCCC TCCCAGAGCG CACGGTGATC
    CAGCTGGATT CCTCGCCCAG GTTTGTGGTC CTATTCAACC CACTGGAACA GGAGCGATTC
    AGCATGGTGT CCCTGCTGGT CAACTCTCCC CGCGTGCGTG TCCTTTCGGA GGAGGGTCAG
    CCCCTGGCCG TGCAGATCAG CGCACACTGG AGCTCTGCCA CCGAGGCGGT CCCTGACGTC
    TACCAGGTGT CTGTGCCTGT CCGCCTGCCA GCCCTGGGCC TGGGCGTGCT GCAGCTACAG
    CTGGGCCTGG ATGGGCACCG CACGCTGCCC TCCTCTGTGC GCATCTACCT GCACGGCCGG
    CAGCTGTCCG TCAGCAGGCA CGAAGCGTTT CCTCTCCGTG TCATTGACTC TGGCACCAGC
    GACTTCGCCC TCAGCAACCG CTACATGCAG GTCTGGTTCT CAGGCCTTAC TGGGCTCCTC
    AAGAGCATCC GAAGGGTGGA TGAGGAGCAC GAGCAGCAGG TGGACATGCA GGTCCTTGTC
    TATGGCACCC GTACGTCCAA AGACAAGAGT GGAGCCTACC TCTTCCTGCC CGATGGCGAG
    GCCAAGCCCT ACGTCCCCAA GGAGCCCCCC GTGCTGCGTG TCACTGAAGG CCCTTTCTTC
    TCAGAGGTGG TTGCGTACTA TGAGCACATT CACCAGGCGG TCCGGCTTTA CAATCTGCCA
    GGGGTGGAGG GGCTGTCTCT GGACATATCA TCCCTGGTGG ACATCCGGGA CTACGTCAAC
    AAGGAGCTGG CCCTGCACAT CCATACAGAC ATCGACAGCC AGGGTATCTT CTTCACAGAC
    CTCAATGGCT TTCAGGTGCA GCCCCGACGG TATCTGAAGA AGCTCCCCCT CCAGGCCAAC
    TTCTACCCCA TGCCAGTCAT GGCCTATATC CAGGACGCAC AGAAGCGCCT CACGCTGCAC
    ACTGCCCAGG CCCTGGGTGT CTCTAGCCTC AAAGATGGCC AGCTGGAGGT GATCTTGGAC
    CGGCGGCTGA TGCAGGATGA CAACCGGGGC CTAGGCCAAG GGCTCAAGGA CAACAAGAGA
    ACCTGCAACC GTTTCCGCCT CCTGCTAGAG CGGCGAACCG TGGGCAGTGA GGTCCAAGAT
    AGCCACTCTA CCAGCTACCC ATCCCTCCTC AGCCACCTGA CCTCCATGTA CCTGAACGCC
    CCGGCGCTCG CTCTGCCTGT AGCCAGGATG CAGCTCCCAG GCCCTGGTCT GCGCTCATTT
    CATCCTCTGG CTTCCTCACT GCCCTGTGAC TTCCACCTGC TCAACCTACG TACGCTCCAG
    GCTGAGGAGG ACACCCTACC CTCGGCGGAG ACCGCACTCA TCTTACACCG CAAGGGTTTT
    GACTGCGGCC TGGAGGCCAA GAACTTGGGC TTCAACTGCA CCACAAGCCA AGGCAAGGTA
    GCCCTGGGCA GCCTTTTCCA TGGCCTGGAT GTGGTATTCC TTCAGCCAAC CTCCTTGACG
    TTACTGTACC CTCTGGCCTC CCCGTCCAAC AGCACTGACG TCTATTTGGA GCCCATGGAG
    ATTGCTACCT TTCGCCTCCG CTTGGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519868.1
    CDS324..2513
    Translation

    Target ORF information:

    RefSeq Version XM_011521566.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521566.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGACGTCCC CCATACCTGT GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC 
    GCCTGCCTGG TGGGCGCATC AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG
    CCAACGTGGC AGAGAGGGCA GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT
    TCCGAAGCAA CGTCCTCCTG GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG
    AGTGGGATGC CCAGTTCTTC AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA
    ACCTCCATGT GCAGGGGGGC AGAGGTTCTG TACAGCCTGG CTGCAGCTCA CGCTCGCCGC
    TCTGGTCTGG CTGGCCGGTA CCCACTGTCT GATTTCACCC TCCTGACGGA AGCTCGGCGC
    ACATTGGGGC TCTTCCAGCA TCACGATGCC ATCACTGGCA CGGCCAAGGA GGCTGTGGTG
    GTGGACTATG GGGTCAGGCT TCTGCGCTCC CTTGTCAACC TGAAGCAGGT CATCATTCAT
    GCAGCCCACT ATCTGGTGCT GGGGGACAAG GAGACCTACC ACTTTGACCC TGAGGCGCCC
    TTCCTCCAAG TGGATGACAC TCGCTTAAGT CACGACGCCC TCCCAGAGCG CACGGTGATC
    CAGCTGGATT CCTCGCCCAG GTTTGTGGTC CTATTCAACC CACTGGAACA GGAGCGATTC
    AGCATGGTGT CCCTGCTGGT CAACTCTCCC CGCGTGCGTG TCCTTTCGGA GGAGGGTCAG
    CCCCTGGCCG TGCAGATCAG CGCACACTGG AGCTCTGCCA CCGAGGCGGT CCCTGACGTC
    TACCAGGTGT CTGTGCCTGT CCGCCTGCCA GCCCTGGGCC TGGGCGTGCT GCAGCTACAG
    CTGGGCCTGG ATGGGCACCG CACGCTGCCC TCCTCTGTGC GCATCTACCT GCACGGCCGG
    CAGCTGTCCG TCAGCAGGCA CGAAGCGTTT CCTCTCCGTG TCATTGACTC TGGCACCAGC
    GACTTCGCCC TCAGCAACCG CTACATGCAG GTCTGGTTCT CAGGCCTTAC TGGGCTCCTC
    AAGAGCATCC GAAGGGTGGA TGAGGAGCAC GAGCAGCAGG TGGACATGCA GGTCCTTGTC
    TATGGCACCC GTACGTCCAA AGACAAGAGT GGAGCCTACC TCTTCCTGCC CGATGGCGAG
    GCCAAGCCCT ACGTCCCCAA GGAGCCCCCC GTGCTGCGTG TCACTGAAGG CCCTTTCTTC
    TCAGAGGTGG TTGCGTACTA TGAGCACATT CACCAGGCGG TCCGGCTTTA CAATCTGCCA
    GGGGTGGAGG GGCTGTCTCT GGACATATCA TCCCTGGTGG ACATCCGGGA CTACGTCAAC
    AAGGAGCTGG CCCTGCACAT CCATACAGAC ATCGACAGCC AGGGTATCTT CTTCACAGAC
    CTCAATGGCT TTCAGGTGCA GCCCCGACGG TATCTGAAGA AGCTCCCCCT CCAGGCCAAC
    TTCTACCCCA TGCCAGTCAT GGCCTATATC CAGGACGCAC AGAAGCGCCT CACGCTGCAC
    ACTGCCCAGG CCCTGGGTGT CTCTAGCCTC AAAGATGGCC AGCTGGAGGT GATCTTGGAC
    CGGCGGCTGA TGCAGGATGA CAACCGGGGC CTAGGCCAAG GGCTCAAGGA CAACAAGAGA
    ACCTGCAACC GTTTCCGCCT CCTGCTAGAG CGGCGAACCG TGGGCAGTGA GGTCCAAGAT
    AGCCACTCTA CCAGCTACCC ATCCCTCCTC AGCCACCTGA CCTCCATGTA CCTGAACGCC
    CCGGCGCTCG CTCTGCCTGT AGCCAGGATG CAGCTCCCAG GCCCTGGTCT GCGCTCATTT
    CATCCTCTGG CTTCCTCACT GCCCTGTGAC TTCCACCTGC TCAACCTACG TACGCTCCAG
    GCTGAGGAGG ACACCCTACC CTCGGCGGAG ACCGCACTCA TCTTACACCG CAAGGGTTTT
    GACTGCGGCC TGGAGGCCAA GAACTTGGGC TTCAACTGCA CCACAAGCCA AGGCAAGGTA
    GCCCTGGGCA GCCTTTTCCA TGGCCTGGAT GTGGTATTCC TTCAGCCAAC CTCCTTGACG
    TTACTGTACC CTCTGGCCTC CCCGTCCAAC AGCACTGACG TCTATTTGGA GCCCATGGAG
    ATTGCTACCT TTCGCCTCCG CTTGGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu30881
    Clone ID Related Accession (Same CDS sequence) XM_017022185.1 , NM_006122.4 , XM_024449922.1 , XM_024449923.1 , XM_024449924.1 , NM_006122.3
    Accession Version NM_006122.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-02
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2x isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC067986.24, AK289863.1, AC068831.17 and AI912006.1. On Nov 22, 2018 this sequence version replaced NM_006122.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.52708.1, AK289863.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000559717.6/ ENSP00000452948.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA
    GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ATGCAGCTCC CAGGCCCTGG TCTGCGCTCA
    TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACGCTC
    CAGGCTGAGG AGGACACCCT ACCCTCGGCG GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG
    GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG
    ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG
    GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006113.2
    CDS150..3602
    Translation

    Target ORF information:

    RefSeq Version NM_006122.4
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006122.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA
    GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ATGCAGCTCC CAGGCCCTGG TCTGCGCTCA
    TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACGCTC
    CAGGCTGAGG AGGACACCCT ACCCTCGGCG GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG
    GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG
    ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG
    GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu30881
    Clone ID Related Accession (Same CDS sequence) XM_017022185.1 , NM_006122.4 , XM_024449922.1 , XM_024449923.1 , XM_024449924.1 , NM_006122.3
    Accession Version NM_006122.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2x isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC067986.24, AK289863.1, AC068831.17 and AI912006.1. On Mar 12, 2016 this sequence version replaced NM_006122.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.52708.1, AK289863.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA
    GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ATGCAGCTCC CAGGCCCTGG TCTGCGCTCA
    TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACGCTC
    CAGGCTGAGG AGGACACCCT ACCCTCGGCG GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG
    GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG
    ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG
    GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006113.2
    CDS221..3673
    Translation

    Target ORF information:

    RefSeq Version NM_006122.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006122.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA
    GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ATGCAGCTCC CAGGCCCTGG TCTGCGCTCA
    TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACGCTC
    CAGGCTGAGG AGGACACCCT ACCCTCGGCG GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG
    GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG
    ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG
    GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48296
    Clone ID Related Accession (Same CDS sequence) NM_001320977.2 , XM_024449920.1 , XM_024449921.1 , XM_005254910.3 , NM_001320977.1 , XM_017022184.1
    Accession Version NM_001320977.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2x isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC067986.24 and AC068831.17. On May 31, 2019 this sequence version replaced NM_001320977.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.98506.1, SRR1803611.55345.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT
    AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGATGCA GCTCCCAGGC
    CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC
    AACCTACGTA CGCTCCAGGC TGAGGAGGAC ACCCTACCCT CGGCGGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC
    ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT
    CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC
    TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001307906.1
    CDS366..3893
    Translation

    Target ORF information:

    RefSeq Version NM_001320977.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001320977.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT
    AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGATGCA GCTCCCAGGC
    CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC
    AACCTACGTA CGCTCCAGGC TGAGGAGGAC ACCCTACCCT CGGCGGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC
    ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT
    CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC
    TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48296
    Clone ID Related Accession (Same CDS sequence) NM_001320977.2 , XM_024449920.1 , XM_024449921.1 , XM_005254910.3 , NM_001320977.1 , XM_017022184.1
    Accession Version NM_001320977.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-04
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2x isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC067986.24 and AC068831.17. On Mar 12, 2016 this sequence version replaced XM_011521562.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.98506.1, SRR1803611.55345.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT
    AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGATGCA GCTCCCAGGC
    CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC
    AACCTACGTA CGCTCCAGGC TGAGGAGGAC ACCCTACCCT CGGCGGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC
    ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT
    CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC
    TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001307906.1
    CDS530..4057
    Translation

    Target ORF information:

    RefSeq Version NM_001320977.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001320977.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT
    AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGATGCA GCTCCCAGGC
    CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC
    AACCTACGTA CGCTCCAGGC TGAGGAGGAC ACCCTACCCT CGGCGGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC
    ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT
    CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC
    TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu30881
    Clone ID Related Accession (Same CDS sequence) XM_017022185.1 , NM_006122.4 , XM_024449922.1 , XM_024449923.1 , XM_024449924.1 , NM_006122.3
    Accession Version XM_017022185.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2x isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA
    GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ATGCAGCTCC CAGGCCCTGG TCTGCGCTCA
    TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACGCTC
    CAGGCTGAGG AGGACACCCT ACCCTCGGCG GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG
    GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG
    ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG
    GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877674.1
    CDS169..3621
    Translation

    Target ORF information:

    RefSeq Version XM_017022185.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022185.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA
    GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ATGCAGCTCC CAGGCCCTGG TCTGCGCTCA
    TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACGCTC
    CAGGCTGAGG AGGACACCCT ACCCTCGGCG GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG
    GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG
    ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG
    GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu30881
    Clone ID Related Accession (Same CDS sequence) XM_017022185.1 , NM_006122.4 , XM_024449922.1 , XM_024449923.1 , XM_024449924.1 , NM_006122.3
    Accession Version XM_024449922.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2x isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA
    GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ATGCAGCTCC CAGGCCCTGG TCTGCGCTCA
    TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACGCTC
    CAGGCTGAGG AGGACACCCT ACCCTCGGCG GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG
    GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG
    ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG
    GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024305690.1
    CDS319..3771
    Translation

    Target ORF information:

    RefSeq Version XM_024449922.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024449922.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA
    GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ATGCAGCTCC CAGGCCCTGG TCTGCGCTCA
    TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACGCTC
    CAGGCTGAGG AGGACACCCT ACCCTCGGCG GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG
    GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG
    ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG
    GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu30881
    Clone ID Related Accession (Same CDS sequence) XM_017022185.1 , NM_006122.4 , XM_024449922.1 , XM_024449923.1 , XM_024449924.1 , NM_006122.3
    Accession Version XM_024449923.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2x isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA
    GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ATGCAGCTCC CAGGCCCTGG TCTGCGCTCA
    TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACGCTC
    CAGGCTGAGG AGGACACCCT ACCCTCGGCG GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG
    GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG
    ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG
    GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024305691.1
    CDS358..3810
    Translation

    Target ORF information:

    RefSeq Version XM_024449923.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024449923.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA
    GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ATGCAGCTCC CAGGCCCTGG TCTGCGCTCA
    TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACGCTC
    CAGGCTGAGG AGGACACCCT ACCCTCGGCG GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG
    GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG
    ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG
    GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu30881
    Clone ID Related Accession (Same CDS sequence) XM_017022185.1 , NM_006122.4 , XM_024449922.1 , XM_024449923.1 , XM_024449924.1 , NM_006122.3
    Accession Version XM_024449924.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2x isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA
    GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ATGCAGCTCC CAGGCCCTGG TCTGCGCTCA
    TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACGCTC
    CAGGCTGAGG AGGACACCCT ACCCTCGGCG GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG
    GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG
    ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG
    GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024305692.1
    CDS289..3741
    Translation

    Target ORF information:

    RefSeq Version XM_024449924.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024449924.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA
    GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ATGCAGCTCC CAGGCCCTGG TCTGCGCTCA
    TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACGCTC
    CAGGCTGAGG AGGACACCCT ACCCTCGGCG GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG
    GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG
    ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG
    GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48296
    Clone ID Related Accession (Same CDS sequence) NM_001320977.2 , XM_024449920.1 , XM_024449921.1 , XM_005254910.3 , NM_001320977.1 , XM_017022184.1
    Accession Version XM_024449920.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2x isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT
    AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGATGCA GCTCCCAGGC
    CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC
    AACCTACGTA CGCTCCAGGC TGAGGAGGAC ACCCTACCCT CGGCGGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC
    ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT
    CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC
    TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024305688.1
    CDS459..3986
    Translation

    Target ORF information:

    RefSeq Version XM_024449920.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024449920.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT
    AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGATGCA GCTCCCAGGC
    CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC
    AACCTACGTA CGCTCCAGGC TGAGGAGGAC ACCCTACCCT CGGCGGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC
    ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT
    CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC
    TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48296
    Clone ID Related Accession (Same CDS sequence) NM_001320977.2 , XM_024449920.1 , XM_024449921.1 , XM_005254910.3 , NM_001320977.1 , XM_017022184.1
    Accession Version XM_024449921.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2x isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT
    AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGATGCA GCTCCCAGGC
    CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC
    AACCTACGTA CGCTCCAGGC TGAGGAGGAC ACCCTACCCT CGGCGGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC
    ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT
    CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC
    TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024305689.1
    CDS289..3816
    Translation

    Target ORF information:

    RefSeq Version XM_024449921.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024449921.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT
    AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGATGCA GCTCCCAGGC
    CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC
    AACCTACGTA CGCTCCAGGC TGAGGAGGAC ACCCTACCCT CGGCGGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC
    ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT
    CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC
    TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48296
    Clone ID Related Accession (Same CDS sequence) NM_001320977.2 , XM_024449920.1 , XM_024449921.1 , XM_005254910.3 , NM_001320977.1 , XM_017022184.1
    Accession Version XM_005254910.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2x isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254910.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT
    AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGATGCA GCTCCCAGGC
    CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC
    AACCTACGTA CGCTCCAGGC TGAGGAGGAC ACCCTACCCT CGGCGGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC
    ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT
    CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC
    TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005254967.1
    CDS169..3696
    Translation

    Target ORF information:

    RefSeq Version XM_005254910.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005254910.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT
    AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGATGCA GCTCCCAGGC
    CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC
    AACCTACGTA CGCTCCAGGC TGAGGAGGAC ACCCTACCCT CGGCGGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC
    ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT
    CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC
    TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48296
    Clone ID Related Accession (Same CDS sequence) NM_001320977.2 , XM_024449920.1 , XM_024449921.1 , XM_005254910.3 , NM_001320977.1 , XM_017022184.1
    Accession Version XM_017022184.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2x isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT
    AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGATGCA GCTCCCAGGC
    CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC
    AACCTACGTA CGCTCCAGGC TGAGGAGGAC ACCCTACCCT CGGCGGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC
    ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT
    CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC
    TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877673.1
    CDS72..3599
    Translation

    Target ORF information:

    RefSeq Version XM_017022184.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022184.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
    TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT
    TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG
    CCACCGGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCGC AGGACTGCCA GTTTGCTTTG
    GGGGGCCGGG GTCAGAAGCC AGAGCTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT
    GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC
    TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC
    GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC
    TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA
    ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG
    CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC
    AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT
    GGCCCAGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC
    AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA
    GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCAGCTGT TCCGAAGCAA CGTCCTCCTG
    GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC
    AACTACCAAC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG
    TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG
    GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG
    GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC
    CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG
    CGCACATTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT
    CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG
    CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG
    ATCCAGCTGG ATTCCTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA
    TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC
    GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA
    CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC
    CGGCAGCTGT CCGTCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC
    AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT
    GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC
    GAGGCCAAGC CCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCGTA CTATGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC
    AACAAGGAGC TGGCCCTGCA CATCCATACA GACATCGACA GCCAGGGTAT CTTCTTCACA
    GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC
    AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG
    CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG
    GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT
    AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGATGCA GCTCCCAGGC
    CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC
    AACCTACGTA CGCTCCAGGC TGAGGAGGAC ACCCTACCCT CGGCGGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC
    ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT
    CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC
    TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.