NCKAP5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NCKAP5
Entrez Gene ID	344148
Full Name	NCK associated protein 5
Synonyms	ERIH1,ERIH2,NAP5
General protein information	Preferred Names NCK associated protein 5 Names nck-associated protein 5 NAP-5 peripheral clock protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q21.2
Disorder MIM:	608789

mRNA and Protein(s)

mRNA	Protein	Name
XM_011511097.2	XP_011509399.1	nck-associated protein 5 isoform X1
XM_017003975.1	XP_016859464.1	nck-associated protein 5 isoform X1
NM_207481.3	NP_997364.3	nck-associated protein 5 isoform 2
XM_005263659.4	XP_005263716.1	nck-associated protein 5 isoform X1
XM_017003975.2	XP_016859464.1	nck-associated protein 5 isoform X1
XM_017003976.2	XP_016859465.1	nck-associated protein 5 isoform X2
XM_011511102.2	XP_011509404.1	nck-associated protein 5 isoform X2
NM_207363.2	NP_997246.2	nck-associated protein 5 isoform 1
XM_017003977.1	XP_016859466.1	nck-associated protein 5 isoform X2
XM_017003978.1	XP_016859467.1	nck-associated protein 5 isoform X4
XM_011511104.1	XP_011509406.1	nck-associated protein 5 isoform X3
XM_011511105.2	XP_011509407.1	nck-associated protein 5 isoform X3
XM_017003980.1	XP_016859469.1	nck-associated protein 5 isoform X6
NM_207363.3	NP_997246.2	nck-associated protein 5 isoform 1
XM_011511099.3	XP_011509401.1	nck-associated protein 5 isoform X1
XM_017003974.1	XP_016859463.1	nck-associated protein 5 isoform X1
XM_017003977.2	XP_016859466.1	nck-associated protein 5 isoform X2
XM_017003979.2	XP_016859468.1	nck-associated protein 5 isoform X5
XM_017003979.1	XP_016859468.1	nck-associated protein 5 isoform X5
XM_011511098.2	XP_011509400.1	nck-associated protein 5 isoform X1
XM_011511099.2	XP_011509401.1	nck-associated protein 5 isoform X1
XM_005263659.3	XP_005263716.1	nck-associated protein 5 isoform X1
XM_005263660.3	XP_005263717.1	nck-associated protein 5 isoform X1
XM_011511097.3	XP_011509399.1	nck-associated protein 5 isoform X1
XM_011511100.3	XP_011509402.1	nck-associated protein 5 isoform X1
XM_011511103.2	XP_011509405.1	nck-associated protein 5 isoform X3
XM_011511100.2	XP_011509402.1	nck-associated protein 5 isoform X1
XM_017003976.1	XP_016859465.1	nck-associated protein 5 isoform X2
NM_207481.4	NP_997364.3	nck-associated protein 5 isoform 2
XM_005263660.4	XP_005263717.1	nck-associated protein 5 isoform X1
XM_011511101.1	XP_011509403.1	nck-associated protein 5 isoform X2
XM_017003978.2	XP_016859467.1	nck-associated protein 5 isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	NCKAP5	NP_997246.2
Canis lupus familiaris (dog)	NCKAP5	XP_005631978.1
Rattus norvegicus (Norway rat)	Nckap5	XP_344135.4
Gallus gallus (chicken)	NCKAP5	XP_422129.4
Bos taurus (cattle)	NCKAP5	XP_002685464.2
Pan troglodytes (chimpanzee)	NCKAP5	XP_001151197.2
	NCKAP5	XP_001102812.2
Mus musculus (house mouse)	Nckap5	NP_001075225.1

Gene Ontology
Bibliography

Related articles in PubMed

A genome-wide association study of brain lesion distribution in multiple sclerosis.
Gourraud PA, Sdika M, Khankhanian P, Henry RG, Beheshtian A, Matthews PM, Hauser SL, Oksenberg JR, Pelletier D, Baranzini SE
Brain : a journal of neurology136(Pt 4)1012-24(2013 Apr)

Genome-wide association study identifies novel loci associated with concentrations of four plasma phospholipid fatty acids in the de novo lipogenesis pathway: results from the Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) consortium.
Wu JH, Lemaitre RN, Manichaikul A, Guan W, Tanaka T, Foy M, Kabagambe EK, Djousse L, Siscovick D, Fretts AM, Johnson C, King IB, Psaty BM, McKnight B, Rich SS, Chen YD, Nettleton JA, Tang W, Bandinelli S, Jacobs DR Jr, Browning BL, Laurie CC, Gu X, Tsai MY, Steffen LM, Ferrucci L, Fornage M, Mozaffarian D
Circulation. Cardiovascular genetics6(2)171-83(2013 Apr)

Genome-wide association study of HLA-DQB1*06:02 negative essential hypersomnia.
Khor SS, Miyagawa T, Toyoda H, Yamasaki M, Kawamura Y, Tanii H, Okazaki Y, Sasaki T, Lin L, Faraco J, Rico T, Honda Y, Honda M, Mignot E, Tokunaga K
PeerJ1e66(2013)

A genome-wide association study in the Japanese population confirms 9p21 and 14q23 as susceptibility loci for primary open angle glaucoma.
Osman W, Low SK, Takahashi A, Kubo M, Nakamura Y
Human molecular genetics21(12)2836-42(2012 Jun)

A genome-wide meta-analysis identifies novel loci associated with schizophrenia and bipolar disorder.
Wang KS, Liu XF, Aragam N
Schizophrenia research124(1-3)192-9(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Meta-analysis and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: A genome-wide meta-analysis identifies novel loci associated with schizophrenia and bipolar disorder.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Genome-wide association study of bipolar disorder in European American and African American individuals.

The following NCKAP5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCKAP5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17398	NM_207363.3 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu17442	NM_207481.4 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu89363	XM_017003979.2 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu89364	XM_017003980.1 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu36199	XM_017003975.2 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu36199	XM_011511100.3 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu36199	XM_005263660.4 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu36199	XM_005263659.4 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu36199	XM_011511097.3 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu36199	XM_011511098.2 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu36199	XM_011511099.3 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu36199	XM_017003974.1 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu36200	XM_011511105.2 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu36200	XM_011511103.2 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu36200	XM_011511104.1 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu79436	XM_017003976.2 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu79436	XM_011511102.2 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu79436	XM_017003977.2 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu79436	XM_011511101.1 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu89362	XM_017003978.2 Latest version!	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu36199	XM_017003975.1	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu17398	NM_207363.2	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu79436	XM_017003977.1	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu89363	XM_017003979.1	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu36199	XM_011511099.2	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu36199	XM_005263659.3	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu36199	XM_011511097.2	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu36199	XM_011511100.2	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu79436	XM_017003976.1	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu17442	NM_207481.3	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu36199	XM_005263660.3	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu89362	XM_017003978.1	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCKAP5 ( NM_207363.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_997246.2 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17398
Clone ID Related Accession (Same CDS sequence)	NM_207363.2 , NM_207363.3
Accession Version	NM_207363.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK057980.1, AK092189.1, AY946008.1, AK124659.1 and CA438539.1. On Feb 28, 2007 this sequence version replaced NM_207363.1. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
GACAAGGCAC CCAGAATGTG TACGTACTCT GCCAGCGGTG GCAGTAATAG TGACAGTGAC 
CTGGACTATG GAGATAATGG TTTTGGAGCT GGAAGGGGAC AGTTAGTGAA AGCACTGAAG 
AGCGCTGCCC CAGAAATTGA GACAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997246.2
CDS	375..6104
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQDKAPRMCTYSASGGSNSDSDLDYGDNGFGAGRGQLVKALKSAAPEIETT

Target ORF information:

RefSeq Version	NM_207363.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207363.2

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
GACAAGGCAC CCAGAATGTG TACGTACTCT GCCAGCGGTG GCAGTAATAG TGACAGTGAC 
CTGGACTATG GAGATAATGG TTTTGGAGCT GGAAGGGGAC AGTTAGTGAA AGCACTGAAG 
AGCGCTGCCC CAGAAATTGA GACAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( NM_207363.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_997246.2 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant 1, mRNA.

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CloneID	OHu17398
Clone ID Related Accession (Same CDS sequence)	NM_207363.2 , NM_207363.3
Accession Version	NM_207363.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC011243.8, AK057980.1, AK092189.1, AY946008.1, AK124659.1, CA438539.1 and AC010974.9. On Nov 22, 2018 this sequence version replaced NM_207363.2. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000409261.6/ ENSP00000387128.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
GACAAGGCAC CCAGAATGTG TACGTACTCT GCCAGCGGTG GCAGTAATAG TGACAGTGAC 
CTGGACTATG GAGATAATGG TTTTGGAGCT GGAAGGGGAC AGTTAGTGAA AGCACTGAAG 
AGCGCTGCCC CAGAAATTGA GACAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997246.2
CDS	378..6107
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQDKAPRMCTYSASGGSNSDSDLDYGDNGFGAGRGQLVKALKSAAPEIETT

Target ORF information:

RefSeq Version	NM_207363.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207363.3

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
GACAAGGCAC CCAGAATGTG TACGTACTCT GCCAGCGGTG GCAGTAATAG TGACAGTGAC 
CTGGACTATG GAGATAATGG TTTTGGAGCT GGAAGGGGAC AGTTAGTGAA AGCACTGAAG 
AGCGCTGCCC CAGAAATTGA GACAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( NM_207481.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_997364.3 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant 2, mRNA.

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CloneID	OHu17442
Clone ID Related Accession (Same CDS sequence)	NM_207481.3 , NM_207481.4
Accession Version	NM_207481.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK057980.1, AK092189.1, AY946007.1, AK124659.1 and CA438539.1. On Feb 28, 2007 this sequence version replaced NM_207481.2. Transcript Variant: This variant (2) lacks several segments in the coding region but maintains the reading frame, compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGGTAAAAGA GGCAAATGAA AACTTGCAAG AGGATGAAGA CGATGCAGTT 
GCAGATTCTG TATTTCAGAG CCACATCATA GAATCCAACT GCCAGATGAG AACATTGGAC 
AGTGGGATCG GAACCTTTCC ACTCCCAGAC TCGGGAAATC GCTCGACAGG ACGCTACCTA 
TGCCAGCCAG ACTCCCCAGA GGACGCTGAG CCTCTCCTGC CTCTCCAGTC AGCCCTTTCT 
GCAGTTTCTT CCATGAGAGC CCAAACCCTT GAACGTGAAG TGCCTTCCTC CACAGACGGC 
CAGCGCCCTG CAGATAGCGC CATTGTTCAT TCCACATCCG ACCCCATCAT GACCGCCAGA 
GGGATGAGGC CTCTTCAGAG CCGCCTCCCC AAACCAGCTT CCTCAGGAAA AGTCAGTTCC 
CAAAAGCAGA ATGAAGCAGA GCCAAGGCCT CAGACATGCT CATCATTCGG ATATGCTGAA 
GACCCAATGG CAAGCCAGCC GCTTCCAGAC TGGGGGAGTG AAGTTGCTGC CACCGGGACC 
CAGGACAAGG CACCCAGAAT GTGTACGTAC TCTGCCAGCG GTGGCAGTAA TAGTGACAGT 
GACCTGGACT ATGGAGATAA TGGTTTTGGA GCTGGAAGGG GACAGTTAGT GAAAGCACTG 
AAGAGCGCTG CCCCAGAAAT TGAGACAACT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997364.3
CDS	375..2147
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQDKAPRMCTYSASGGSNSDSDLDYGDNGFGAGRGQLVKALKSAAPEIETT

Target ORF information:

RefSeq Version	NM_207481.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207481.3

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGGTAAAAGA GGCAAATGAA AACTTGCAAG AGGATGAAGA CGATGCAGTT 
GCAGATTCTG TATTTCAGAG CCACATCATA GAATCCAACT GCCAGATGAG AACATTGGAC 
AGTGGGATCG GAACCTTTCC ACTCCCAGAC TCGGGAAATC GCTCGACAGG ACGCTACCTA 
TGCCAGCCAG ACTCCCCAGA GGACGCTGAG CCTCTCCTGC CTCTCCAGTC AGCCCTTTCT 
GCAGTTTCTT CCATGAGAGC CCAAACCCTT GAACGTGAAG TGCCTTCCTC CACAGACGGC 
CAGCGCCCTG CAGATAGCGC CATTGTTCAT TCCACATCCG ACCCCATCAT GACCGCCAGA 
GGGATGAGGC CTCTTCAGAG CCGCCTCCCC AAACCAGCTT CCTCAGGAAA AGTCAGTTCC 
CAAAAGCAGA ATGAAGCAGA GCCAAGGCCT CAGACATGCT CATCATTCGG ATATGCTGAA 
GACCCAATGG CAAGCCAGCC GCTTCCAGAC TGGGGGAGTG AAGTTGCTGC CACCGGGACC 
CAGGACAAGG CACCCAGAAT GTGTACGTAC TCTGCCAGCG GTGGCAGTAA TAGTGACAGT 
GACCTGGACT ATGGAGATAA TGGTTTTGGA GCTGGAAGGG GACAGTTAGT GAAAGCACTG 
AAGAGCGCTG CCCCAGAAAT TGAGACAACT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( NM_207481.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_997364.3 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant 2, mRNA.

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CloneID	OHu17442
Clone ID Related Accession (Same CDS sequence)	NM_207481.3 , NM_207481.4
Accession Version	NM_207481.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC011243.8, AK057980.1, AK092189.1, AY946007.1, AK124659.1, CA438539.1 and AC010974.9. On May 31, 2019 this sequence version replaced NM_207481.3. Transcript Variant: This variant (2) lacks several segments in the coding region but maintains the reading frame, compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGGTAAAAGA GGCAAATGAA AACTTGCAAG AGGATGAAGA CGATGCAGTT 
GCAGATTCTG TATTTCAGAG CCACATCATA GAATCCAACT GCCAGATGAG AACATTGGAC 
AGTGGGATCG GAACCTTTCC ACTCCCAGAC TCGGGAAATC GCTCGACAGG ACGCTACCTA 
TGCCAGCCAG ACTCCCCAGA GGACGCTGAG CCTCTCCTGC CTCTCCAGTC AGCCCTTTCT 
GCAGTTTCTT CCATGAGAGC CCAAACCCTT GAACGTGAAG TGCCTTCCTC CACAGACGGC 
CAGCGCCCTG CAGATAGCGC CATTGTTCAT TCCACATCCG ACCCCATCAT GACCGCCAGA 
GGGATGAGGC CTCTTCAGAG CCGCCTCCCC AAACCAGCTT CCTCAGGAAA AGTCAGTTCC 
CAAAAGCAGA ATGAAGCAGA GCCAAGGCCT CAGACATGCT CATCATTCGG ATATGCTGAA 
GACCCAATGG CAAGCCAGCC GCTTCCAGAC TGGGGGAGTG AAGTTGCTGC CACCGGGACC 
CAGGACAAGG CACCCAGAAT GTGTACGTAC TCTGCCAGCG GTGGCAGTAA TAGTGACAGT 
GACCTGGACT ATGGAGATAA TGGTTTTGGA GCTGGAAGGG GACAGTTAGT GAAAGCACTG 
AAGAGCGCTG CCCCAGAAAT TGAGACAACT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997364.3
CDS	378..2150
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQDKAPRMCTYSASGGSNSDSDLDYGDNGFGAGRGQLVKALKSAAPEIETT

Target ORF information:

RefSeq Version	NM_207481.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207481.4

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGGTAAAAGA GGCAAATGAA AACTTGCAAG AGGATGAAGA CGATGCAGTT 
GCAGATTCTG TATTTCAGAG CCACATCATA GAATCCAACT GCCAGATGAG AACATTGGAC 
AGTGGGATCG GAACCTTTCC ACTCCCAGAC TCGGGAAATC GCTCGACAGG ACGCTACCTA 
TGCCAGCCAG ACTCCCCAGA GGACGCTGAG CCTCTCCTGC CTCTCCAGTC AGCCCTTTCT 
GCAGTTTCTT CCATGAGAGC CCAAACCCTT GAACGTGAAG TGCCTTCCTC CACAGACGGC 
CAGCGCCCTG CAGATAGCGC CATTGTTCAT TCCACATCCG ACCCCATCAT GACCGCCAGA 
GGGATGAGGC CTCTTCAGAG CCGCCTCCCC AAACCAGCTT CCTCAGGAAA AGTCAGTTCC 
CAAAAGCAGA ATGAAGCAGA GCCAAGGCCT CAGACATGCT CATCATTCGG ATATGCTGAA 
GACCCAATGG CAAGCCAGCC GCTTCCAGAC TGGGGGAGTG AAGTTGCTGC CACCGGGACC 
CAGGACAAGG CACCCAGAAT GTGTACGTAC TCTGCCAGCG GTGGCAGTAA TAGTGACAGT 
GACCTGGACT ATGGAGATAA TGGTTTTGGA GCTGGAAGGG GACAGTTAGT GAAAGCACTG 
AAGAGCGCTG CCCCAGAAAT TGAGACAACT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_017003979.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859468.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu89363
Clone ID Related Accession (Same CDS sequence)	XM_017003979.2 , XM_017003979.1
Accession Version	XM_017003979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017003979.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGGTAAAAGA GGCAAATGAA AACTTGCAAG AGGATGAAGA CGATGCAGTT 
GCAGATTCTG TATTTCAGAG CCACATCATA GAATCCAACT GCCAGATGAG AACATTGGAC 
AGTGGGATCG GAACCTTTCC ACTCCCAGAC TCGGGAAATC GCTCGACAGG ACGCTACCTA 
TGCCAGCCAG ACTCCCCAGA GGACGCTGAG CCTCTCCTGC CTCTCCAGTC AGCCCTTTCT 
GCAGTTTCTT CCATGAGAGC CCAAACCCTT GAACGTGAAG TGCCTTCCTC CACAGACGGC 
CAGCGCCCTG CAGATAGCGC CATTGTTCAT TCCACATCCG ACCCCATCAT GACCGCCAGA 
GGGATGAGGC CTCTTCAGAG CCGCCTCCCC AAACCAGCTT CCTCAGGAAA AGTCAGTTCC 
CAAAAGCAGA ATGAAGCAGA GCCAAGGCCT CAGACATGCT CATCATTCGG ATATGCTGAA 
GACCCAATGG CAAGCCAGCC GCTTCCAGAC TGGGGGAGTG AAGTTGCTGC CACCGGGACC 
CAGAAATTGA GACAACTTGA AGAAACAAAA GACGATCCCG AGAATAGATT ATCGAAAATT 
TCCCTAGAGT CATTCAATAA ATTTAACAGC AATACTGTGA TTTTATTAGA AAAAGAGAAG 
AACTCTCTGA ACAAGGTTGA AGGACAGAAG GAAGAAAAAG AAAAAAATGA AGAGACATCT 
TTGAGTAGTT CAGATAGGCC TGGGGTAGAC AACTTGGAAT CTTTGAGTGA TTCTTTATAT 
GATAGCTTCT CTTCCTGTGC CAGTCAAGGT TCAAATGATG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859468.1
CDS	1024..2928
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_017003979.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003979.2

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGGTAAAAGA GGCAAATGAA AACTTGCAAG AGGATGAAGA CGATGCAGTT 
GCAGATTCTG TATTTCAGAG CCACATCATA GAATCCAACT GCCAGATGAG AACATTGGAC 
AGTGGGATCG GAACCTTTCC ACTCCCAGAC TCGGGAAATC GCTCGACAGG ACGCTACCTA 
TGCCAGCCAG ACTCCCCAGA GGACGCTGAG CCTCTCCTGC CTCTCCAGTC AGCCCTTTCT 
GCAGTTTCTT CCATGAGAGC CCAAACCCTT GAACGTGAAG TGCCTTCCTC CACAGACGGC 
CAGCGCCCTG CAGATAGCGC CATTGTTCAT TCCACATCCG ACCCCATCAT GACCGCCAGA 
GGGATGAGGC CTCTTCAGAG CCGCCTCCCC AAACCAGCTT CCTCAGGAAA AGTCAGTTCC 
CAAAAGCAGA ATGAAGCAGA GCCAAGGCCT CAGACATGCT CATCATTCGG ATATGCTGAA 
GACCCAATGG CAAGCCAGCC GCTTCCAGAC TGGGGGAGTG AAGTTGCTGC CACCGGGACC 
CAGAAATTGA GACAACTTGA AGAAACAAAA GACGATCCCG AGAATAGATT ATCGAAAATT 
TCCCTAGAGT CATTCAATAA ATTTAACAGC AATACTGTGA TTTTATTAGA AAAAGAGAAG 
AACTCTCTGA ACAAGGTTGA AGGACAGAAG GAAGAAAAAG AAAAAAATGA AGAGACATCT 
TTGAGTAGTT CAGATAGGCC TGGGGTAGAC AACTTGGAAT CTTTGAGTGA TTCTTTATAT 
GATAGCTTCT CTTCCTGTGC CAGTCAAGGT TCAAATGATG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_017003979.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859468.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu89363
Clone ID Related Accession (Same CDS sequence)	XM_017003979.2 , XM_017003979.1
Accession Version	XM_017003979.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGGTAAAAGA GGCAAATGAA AACTTGCAAG AGGATGAAGA CGATGCAGTT 
GCAGATTCTG TATTTCAGAG CCACATCATA GAATCCAACT GCCAGATGAG AACATTGGAC 
AGTGGGATCG GAACCTTTCC ACTCCCAGAC TCGGGAAATC GCTCGACAGG ACGCTACCTA 
TGCCAGCCAG ACTCCCCAGA GGACGCTGAG CCTCTCCTGC CTCTCCAGTC AGCCCTTTCT 
GCAGTTTCTT CCATGAGAGC CCAAACCCTT GAACGTGAAG TGCCTTCCTC CACAGACGGC 
CAGCGCCCTG CAGATAGCGC CATTGTTCAT TCCACATCCG ACCCCATCAT GACCGCCAGA 
GGGATGAGGC CTCTTCAGAG CCGCCTCCCC AAACCAGCTT CCTCAGGAAA AGTCAGTTCC 
CAAAAGCAGA ATGAAGCAGA GCCAAGGCCT CAGACATGCT CATCATTCGG ATATGCTGAA 
GACCCAATGG CAAGCCAGCC GCTTCCAGAC TGGGGGAGTG AAGTTGCTGC CACCGGGACC 
CAGAAATTGA GACAACTTGA AGAAACAAAA GACGATCCCG AGAATAGATT ATCGAAAATT 
TCCCTAGAGT CATTCAATAA ATTTAACAGC AATACTGTGA TTTTATTAGA AAAAGAGAAG 
AACTCTCTGA ACAAGGTTGA AGGACAGAAG GAAGAAAAAG AAAAAAATGA AGAGACATCT 
TTGAGTAGTT CAGATAGGCC TGGGGTAGAC AACTTGGAAT CTTTGAGTGA TTCTTTATAT 
GATAGCTTCT CTTCCTGTGC CAGTCAAGGT TCAAATGATG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859468.1
CDS	1015..2919
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_017003979.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003979.1

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGGTAAAAGA GGCAAATGAA AACTTGCAAG AGGATGAAGA CGATGCAGTT 
GCAGATTCTG TATTTCAGAG CCACATCATA GAATCCAACT GCCAGATGAG AACATTGGAC 
AGTGGGATCG GAACCTTTCC ACTCCCAGAC TCGGGAAATC GCTCGACAGG ACGCTACCTA 
TGCCAGCCAG ACTCCCCAGA GGACGCTGAG CCTCTCCTGC CTCTCCAGTC AGCCCTTTCT 
GCAGTTTCTT CCATGAGAGC CCAAACCCTT GAACGTGAAG TGCCTTCCTC CACAGACGGC 
CAGCGCCCTG CAGATAGCGC CATTGTTCAT TCCACATCCG ACCCCATCAT GACCGCCAGA 
GGGATGAGGC CTCTTCAGAG CCGCCTCCCC AAACCAGCTT CCTCAGGAAA AGTCAGTTCC 
CAAAAGCAGA ATGAAGCAGA GCCAAGGCCT CAGACATGCT CATCATTCGG ATATGCTGAA 
GACCCAATGG CAAGCCAGCC GCTTCCAGAC TGGGGGAGTG AAGTTGCTGC CACCGGGACC 
CAGAAATTGA GACAACTTGA AGAAACAAAA GACGATCCCG AGAATAGATT ATCGAAAATT 
TCCCTAGAGT CATTCAATAA ATTTAACAGC AATACTGTGA TTTTATTAGA AAAAGAGAAG 
AACTCTCTGA ACAAGGTTGA AGGACAGAAG GAAGAAAAAG AAAAAAATGA AGAGACATCT 
TTGAGTAGTT CAGATAGGCC TGGGGTAGAC AACTTGGAAT CTTTGAGTGA TTCTTTATAT 
GATAGCTTCT CTTCCTGTGC CAGTCAAGGT TCAAATGATG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_017003980.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859469.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu89364
Clone ID Related Accession (Same CDS sequence)	XM_017003980.1
Accession Version	XM_017003980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGAGA AGCTGATACA TGAACTGGAA GAGGAGAGAC ACTTACGTCT TCAAAGCGAG 
AAGCGGTTGC AGGAGGTGAC CCTAGAGTCT GAACGCAACA GAATTCAGAT GCGTAGCTTG 
CAGCAGCAGT TCTCCAGGAT GGAAGAAACA GTACGAAATC TATTGCAGAG TCAAGGATCT 
CCAGAGCAGA AAAAAGAAGA AACTGTTAAT ATAATGGTCT ATCAGGAAAA GCTGTCAGAG 
GAAGAGAGAA AACATAAGGA AGCTTTGGAA GATCTTCACA TGGTGGTTGA TGAGGATTCG 
AGGAGTGAAA GCAGCAGTAC AGATGAGGGA AAAGAAAAGA CCAAATTGCT ATTAGAGAGA 
TTGAAAGCTC TAGAGGCAGA GAATTCAGCG TTGGCTTTGG AGAATGAAAA TCAAAGGGAA 
CAATATGAGC GATGTCTTGA TGAGGTAGCC AACCAAGTTG TTCAAGCTCT GCTTACTCAA 
AAGGATCTAA GAGAGGAATG TGTGAAGTTG AAAACAAGAG TGTTTGATTT GGAACAGCAG 
AATCGAACAC TAAGCATCCT ATTCCAACAG CGAGTCAGAC CCACTTCTGA TCTGCTCCTC 
CAGAAACTTC ACTCACGTCT CTTGGATCTT TCATCTGGAG ATTTGCTTTC AGAGGTGGAA 
AGAAACCGAA GTCTGACACA GTCACGAACT GATGCTGAGG TGCACGAACA CCAACTAAAT 
ACAAAATCGG CCTTGAAATG CCCTGGCTTG GGGGCTGTCA TCCCTGGTCA TCTCTGTCCT 
CGAAACAGCT ACAGTAGCAG CAGTGAACTG TCTCTTTCAA GCACCTGCAG CGAGTACTCC 
AGTGGCTCCT CCTACACGTG GCACGATGGG AAGAACCTCA GGAAAAGGGT AAAAGAGGCA 
AATGAAAACT TGCAAGAGGA TGAAGACGAT GCAGTTGCAG ATTCTGTATT TCAGAGCCAC 
ATCATAGAAT CCAACTGCCA GATGAGAACA TTGGACAGTG GGATCGGAAC CTTTCCACTC 
CCAGACTCGG GAAATCGCTC GACAGGACGC TACCTATGCC AGCCAGACTC CCCAGAGGAC 
GCTGAGCCTC TCCTGCCTCT CCAGTCAGCC CTTTCTGCAG TTTCTTCCAT GAGAGCCCAA 
ACCCTTGAAC GTGAAGTGCC TTCCTCCACA GACGGCCAGC GCCCTGCAGA TAGCGCCATT 
GTTCATTCCA CATCCGACCC CATCATGACC GCCAGAGGGA TGAGGCCTCT TCAGAGCCGC 
CTCCCCAAAC CAGCTTCCTC AGGAAAAGTC AGTTCCCAAA AGCAGAATGA AGCAGAGCCA 
AGGCCTCAGA CATGCTCATC ATTCGGATAT GCTGAAGACC CAATGGCAAG CCAGCCGCTT 
CCAGACTGGG GGAGTGAAGT TGCTGCCACC GGGACCCAGG ACAAGGCACC CAGAATGTGT 
ACGTACTCTG CCAGCGGTGG CAGTAATAGT GACAGTGACC TGGACTATGG AGATAATGGT 
TTTGGAGCTG GAAGGGGACA GTTAGTGAAA GCACTGAAGA GCGCTGCCCC AGAAATTGAG 
ACAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859469.1
CDS	480..2048
Translation	MHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQDKAPRMCTYSASGGSNSDSDLDYGDNGFGAGRGQLVKALKSAAPEIETT

Target ORF information:

RefSeq Version	XM_017003980.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003980.1

ORF Insert Sequence:

ATGCATGAGA AGCTGATACA TGAACTGGAA GAGGAGAGAC ACTTACGTCT TCAAAGCGAG 
AAGCGGTTGC AGGAGGTGAC CCTAGAGTCT GAACGCAACA GAATTCAGAT GCGTAGCTTG 
CAGCAGCAGT TCTCCAGGAT GGAAGAAACA GTACGAAATC TATTGCAGAG TCAAGGATCT 
CCAGAGCAGA AAAAAGAAGA AACTGTTAAT ATAATGGTCT ATCAGGAAAA GCTGTCAGAG 
GAAGAGAGAA AACATAAGGA AGCTTTGGAA GATCTTCACA TGGTGGTTGA TGAGGATTCG 
AGGAGTGAAA GCAGCAGTAC AGATGAGGGA AAAGAAAAGA CCAAATTGCT ATTAGAGAGA 
TTGAAAGCTC TAGAGGCAGA GAATTCAGCG TTGGCTTTGG AGAATGAAAA TCAAAGGGAA 
CAATATGAGC GATGTCTTGA TGAGGTAGCC AACCAAGTTG TTCAAGCTCT GCTTACTCAA 
AAGGATCTAA GAGAGGAATG TGTGAAGTTG AAAACAAGAG TGTTTGATTT GGAACAGCAG 
AATCGAACAC TAAGCATCCT ATTCCAACAG CGAGTCAGAC CCACTTCTGA TCTGCTCCTC 
CAGAAACTTC ACTCACGTCT CTTGGATCTT TCATCTGGAG ATTTGCTTTC AGAGGTGGAA 
AGAAACCGAA GTCTGACACA GTCACGAACT GATGCTGAGG TGCACGAACA CCAACTAAAT 
ACAAAATCGG CCTTGAAATG CCCTGGCTTG GGGGCTGTCA TCCCTGGTCA TCTCTGTCCT 
CGAAACAGCT ACAGTAGCAG CAGTGAACTG TCTCTTTCAA GCACCTGCAG CGAGTACTCC 
AGTGGCTCCT CCTACACGTG GCACGATGGG AAGAACCTCA GGAAAAGGGT AAAAGAGGCA 
AATGAAAACT TGCAAGAGGA TGAAGACGAT GCAGTTGCAG ATTCTGTATT TCAGAGCCAC 
ATCATAGAAT CCAACTGCCA GATGAGAACA TTGGACAGTG GGATCGGAAC CTTTCCACTC 
CCAGACTCGG GAAATCGCTC GACAGGACGC TACCTATGCC AGCCAGACTC CCCAGAGGAC 
GCTGAGCCTC TCCTGCCTCT CCAGTCAGCC CTTTCTGCAG TTTCTTCCAT GAGAGCCCAA 
ACCCTTGAAC GTGAAGTGCC TTCCTCCACA GACGGCCAGC GCCCTGCAGA TAGCGCCATT 
GTTCATTCCA CATCCGACCC CATCATGACC GCCAGAGGGA TGAGGCCTCT TCAGAGCCGC 
CTCCCCAAAC CAGCTTCCTC AGGAAAAGTC AGTTCCCAAA AGCAGAATGA AGCAGAGCCA 
AGGCCTCAGA CATGCTCATC ATTCGGATAT GCTGAAGACC CAATGGCAAG CCAGCCGCTT 
CCAGACTGGG GGAGTGAAGT TGCTGCCACC GGGACCCAGG ACAAGGCACC CAGAATGTGT 
ACGTACTCTG CCAGCGGTGG CAGTAATAGT GACAGTGACC TGGACTATGG AGATAATGGT 
TTTGGAGCTG GAAGGGGACA GTTAGTGAAA GCACTGAAGA GCGCTGCCCC AGAAATTGAG 
ACAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_011511097.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509399.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X1, mRNA.

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CloneID	OHu36199
Clone ID Related Accession (Same CDS sequence)	XM_011511097.2 , XM_017003975.1 , XM_005263659.4 , XM_017003975.2 , XM_011511099.3 , XM_017003974.1 , XM_011511098.2 , XM_011511099.2 , XM_005263659.3 , XM_005263660.3 , XM_011511097.3 , XM_011511100.3 , XM_011511100.2 , XM_005263660.4
Accession Version	XM_011511097.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511097.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509399.1
CDS	1145..7006
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_011511097.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511097.2

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_017003975.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859464.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X7, mRNA.

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CloneID	OHu36199
Clone ID Related Accession (Same CDS sequence)	XM_011511097.2 , XM_017003975.1 , XM_005263659.4 , XM_017003975.2 , XM_011511099.3 , XM_017003974.1 , XM_011511098.2 , XM_011511099.2 , XM_005263659.3 , XM_005263660.3 , XM_011511097.3 , XM_011511100.3 , XM_011511100.2 , XM_005263660.4
Accession Version	XM_017003975.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859464.1
CDS	570..6431
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_017003975.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003975.1

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_005263659.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263716.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X5, mRNA.

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CloneID	OHu36199
Clone ID Related Accession (Same CDS sequence)	XM_011511097.2 , XM_017003975.1 , XM_005263659.4 , XM_017003975.2 , XM_011511099.3 , XM_017003974.1 , XM_011511098.2 , XM_011511099.2 , XM_005263659.3 , XM_005263660.3 , XM_011511097.3 , XM_011511100.3 , XM_011511100.2 , XM_005263660.4
Accession Version	XM_005263659.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263659.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263716.1
CDS	1102..6963
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_005263659.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263659.4

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_017003975.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859464.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X6, mRNA.

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CloneID	OHu36199
Clone ID Related Accession (Same CDS sequence)	XM_011511097.2 , XM_017003975.1 , XM_005263659.4 , XM_017003975.2 , XM_011511099.3 , XM_017003974.1 , XM_011511098.2 , XM_011511099.2 , XM_005263659.3 , XM_005263660.3 , XM_011511097.3 , XM_011511100.3 , XM_011511100.2 , XM_005263660.4
Accession Version	XM_017003975.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017003975.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859464.1
CDS	571..6432
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_017003975.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003975.2

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_011511099.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509401.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X2, mRNA.

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CloneID	OHu36199
Clone ID Related Accession (Same CDS sequence)	XM_011511097.2 , XM_017003975.1 , XM_005263659.4 , XM_017003975.2 , XM_011511099.3 , XM_017003974.1 , XM_011511098.2 , XM_011511099.2 , XM_005263659.3 , XM_005263660.3 , XM_011511097.3 , XM_011511100.3 , XM_011511100.2 , XM_005263660.4
Accession Version	XM_011511099.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011511099.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509401.1
CDS	541..6402
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_011511099.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511099.3

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_017003974.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859463.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X4, mRNA.

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CloneID	OHu36199
Clone ID Related Accession (Same CDS sequence)	XM_011511097.2 , XM_017003975.1 , XM_005263659.4 , XM_017003975.2 , XM_011511099.3 , XM_017003974.1 , XM_011511098.2 , XM_011511099.2 , XM_005263659.3 , XM_005263660.3 , XM_011511097.3 , XM_011511100.3 , XM_011511100.2 , XM_005263660.4
Accession Version	XM_017003974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859463.1
CDS	268..6129
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_017003974.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003974.1

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_011511098.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509400.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X2, mRNA.

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CloneID	OHu36199
Clone ID Related Accession (Same CDS sequence)	XM_011511097.2 , XM_017003975.1 , XM_005263659.4 , XM_017003975.2 , XM_011511099.3 , XM_017003974.1 , XM_011511098.2 , XM_011511099.2 , XM_005263659.3 , XM_005263660.3 , XM_011511097.3 , XM_011511100.3 , XM_011511100.2 , XM_005263660.4
Accession Version	XM_011511098.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511098.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509400.1
CDS	1142..7003
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_011511098.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511098.2

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_011511099.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509401.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X3, mRNA.

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CloneID	OHu36199
Clone ID Related Accession (Same CDS sequence)	XM_011511097.2 , XM_017003975.1 , XM_005263659.4 , XM_017003975.2 , XM_011511099.3 , XM_017003974.1 , XM_011511098.2 , XM_011511099.2 , XM_005263659.3 , XM_005263660.3 , XM_011511097.3 , XM_011511100.3 , XM_011511100.2 , XM_005263660.4
Accession Version	XM_011511099.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511099.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509401.1
CDS	540..6401
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_011511099.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511099.2

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_005263659.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263716.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu36199
Clone ID Related Accession (Same CDS sequence)	XM_011511097.2 , XM_017003975.1 , XM_005263659.4 , XM_017003975.2 , XM_011511099.3 , XM_017003974.1 , XM_011511098.2 , XM_011511099.2 , XM_005263659.3 , XM_005263660.3 , XM_011511097.3 , XM_011511100.3 , XM_011511100.2 , XM_005263660.4
Accession Version	XM_005263659.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263659.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263716.1
CDS	957..6818
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_005263659.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263659.3

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_005263660.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263717.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X8, mRNA.

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CloneID	OHu36199
Clone ID Related Accession (Same CDS sequence)	XM_011511097.2 , XM_017003975.1 , XM_005263659.4 , XM_017003975.2 , XM_011511099.3 , XM_017003974.1 , XM_011511098.2 , XM_011511099.2 , XM_005263659.3 , XM_005263660.3 , XM_011511097.3 , XM_011511100.3 , XM_011511100.2 , XM_005263660.4
Accession Version	XM_005263660.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263660.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263717.1
CDS	472..6333
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_005263660.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263660.3

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_011511097.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509399.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu36199
Clone ID Related Accession (Same CDS sequence)	XM_011511097.2 , XM_017003975.1 , XM_005263659.4 , XM_017003975.2 , XM_011511099.3 , XM_017003974.1 , XM_011511098.2 , XM_011511099.2 , XM_005263659.3 , XM_005263660.3 , XM_011511097.3 , XM_011511100.3 , XM_011511100.2 , XM_005263660.4
Accession Version	XM_011511097.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011511097.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509399.1
CDS	1290..7151
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_011511097.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511097.3

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_011511100.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509402.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X3, mRNA.

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CloneID	OHu36199
Clone ID Related Accession (Same CDS sequence)	XM_011511097.2 , XM_017003975.1 , XM_005263659.4 , XM_017003975.2 , XM_011511099.3 , XM_017003974.1 , XM_011511098.2 , XM_011511099.2 , XM_005263659.3 , XM_005263660.3 , XM_011511097.3 , XM_011511100.3 , XM_011511100.2 , XM_005263660.4
Accession Version	XM_011511100.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011511100.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509402.1
CDS	639..6500
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_011511100.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511100.3

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_011511100.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509402.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X4, mRNA.

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CloneID	OHu36199
Clone ID Related Accession (Same CDS sequence)	XM_011511097.2 , XM_017003975.1 , XM_005263659.4 , XM_017003975.2 , XM_011511099.3 , XM_017003974.1 , XM_011511098.2 , XM_011511099.2 , XM_005263659.3 , XM_005263660.3 , XM_011511097.3 , XM_011511100.3 , XM_011511100.2 , XM_005263660.4
Accession Version	XM_011511100.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511100.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509402.1
CDS	638..6499
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_011511100.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511100.2

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_005263660.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263717.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X7, mRNA.

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CloneID	OHu36199
Clone ID Related Accession (Same CDS sequence)	XM_011511097.2 , XM_017003975.1 , XM_005263659.4 , XM_017003975.2 , XM_011511099.3 , XM_017003974.1 , XM_011511098.2 , XM_011511099.2 , XM_005263659.3 , XM_005263660.3 , XM_011511097.3 , XM_011511100.3 , XM_011511100.2 , XM_005263660.4
Accession Version	XM_005263660.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263660.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263717.1
CDS	473..6334
Translation	MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_005263660.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263660.4

ORF Insert Sequence:

ATGGAGGGAA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TCTAGACAGC 
AGTCTTGTGG AATACATGGA CTCCAATAAA TACATTGAGC ATCTGCTGAC TCAGCTTGAG 
GAGCAACACA GGAGTCTCTG GAGGGAGAAG TTGGCAGTGG CCCGACTACA GCGAGAAGTT 
GCCCAAAGAA CAAGTGAGGG GGCCATGCAT GAGAAGCTGA TACATGAACT GGAAGAGGAG 
AGACACTTAC GTCTTCAAAG CGAGAAGCGG TTGCAGGAGG TGACCCTAGA GTCTGAACGC 
AACAGAATTC AGATGCGTAG CTTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA 
AATCTATTGC AGAGTCAAGG ATCTCCAGAG CAGAAAAAAG AAGAAACTGT TAATATAATG 
GTCTATCAGG AAAAGCTGTC AGAGGAAGAG AGAAAACATA AGGAAGCTTT GGAAGATCTT 
CACATGGTGG TTGATGAGGA TTCGAGGAGT GAAAGCAGCA GTACAGATGA GGGAAAAGAA 
AAGACCAAAT TGCTATTAGA GAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCGTTGGCT 
TTGGAGAATG AAAATCAAAG GGAACAATAT GAGCGATGTC TTGATGAGGT AGCCAACCAA 
GTTGTTCAAG CTCTGCTTAC TCAAAAGGAT CTAAGAGAGG AATGTGTGAA GTTGAAAACA 
AGAGTGTTTG ATTTGGAACA GCAGAATCGA ACACTAAGCA TCCTATTCCA ACAGCGAGTC 
AGACCCACTT CTGATCTGCT CCTCCAGAAA CTTCACTCAC GTCTCTTGGA TCTTTCATCT 
GGAGATTTGC TTTCAGAGGT GGAAAGAAAC CGAAGTCTGA CACAGTCACG AACTGATGCT 
GAGGTGCACG AACACCAACT AAATACAAAA TCGGCCTTGA AATGCCCTGG CTTGGGGGCT 
GTCATCCCTG GTCATCTCTG TCCTCGAAAC AGCTACAGTA GCAGCAGTGA ACTGTCTCTT 
TCAAGCACCT GCAGCGAGTA CTCCAGTGGC TCCTCCTACA CGTGGCACGA TGGGAAGAAC 
CTCAGGAAAA GGCAATCATC ACAAAACTGG GATAAAAGGC TAAGTATTGA TTCTTCGCTC 
CCAAGTGGCT TTGCTAGTCC TACAAATGAA CTACCTCCAA CTCGTATCAA GGAAAGCCAC 
ATTTTGGAAG GGCTAAGAAA GCTACAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG 
ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCGAATG AAGGAATATA TTCTCCTGGA 
ATTAAAAGTA GCAGCCTCAA GGAGTATCCC CCCTGCAAAA CAGCTGACCT GGGGAGCCCC 
TGCAAGGAAC CCCACAAGAC ATTTGTTTAT GATCTAGATT CCCACGTTGA TGCGGACGAT 
GACCCTTCCA CCTTAGCATT GCTCCAAGCA GTTCCAAACC AGAGCTGCAG GCCACATGGC 
AGTAAATTAA CCCACAGTGT TTCCGACAGT CTGTTTGGCT GGGAGACAAA CAGAAAACAC 
TTTCTGGAAG GCACATCCTC AGTTTATCCC AAGGAAAGGC CTGAAAAGCT GACAAGTTGC 
GCCAGCAGCT GTCCCTTAGA GATGAAGCTG TGCCCAAGTG TGCAGACGCC TCAGGTACAG 
AGGGAGAGGG GCCCACAGGG CCAAGGCCAT GGCCGCATGG CTCTCAACCT CCAGCTTTCA 
GACACTGATG ACAATGAAAC GTTTGATGAG CTGCACATAG AGAGCAGTGA TGAGAAAAGT 
CCTTCAGACG TGTCATTGGC TGCCGACACC GATAAGTCCG TGGAGAACCT GGATGTCCTT 
GTGGGGTTTG GAAAATCTCT ATGTGGGTCT CCTGAAGAGG AGGAAAAACA AGTGCCCATC 
CCTTCAGAGA CTAGGCCAAA GACTTTTAGT TTCATTAAGC AGCAAAGAGT TGTAAAAAGG 
ACTTCTTCAG AAGAATGTGT GACTGTGATA TTTGATGCGG AAGACGGTGA GCCCATTGAA 
TTCAGCTCTC ACCAGACTGG GGTTGTCACT GTTACCAGAA ATGAGATTTC CATCAATTCA 
ACTCCTGCTG GACCCAAGGC AGAACATACT GAGCTTTTAC CTCAGGGAAT TGCTTGTTTA 
CAGCCAAGAG CTGCTGCAAG AGACTATACT TTCTTTAAAA GGTCTGAAGA GGACACTGAG 
AAAAACATTC CAAAAGATAA TGTAGATAAT GTTCCCAGGG TGTCCACTGA ATCTTTCAGC 
TCCAGGACAG TGACACAAAA TCCTCAGCAG CAAAAGCTGG TCAAACCAAC ACACAATATA 
TCATGCCAGA GTAATTCCAG GTCTTCGGCA CCCATGGGCA TCTATCAAAA GCAAAATCTG 
ACAAAAATAC CTCCCAGGGG CAAGTCTTCA CCTCAGAAAT CAAAACTAAT GGAGCCCGAA 
GCCACCACAC TACTCCCTTC ATCTGGCCTG GTGACTCTTG AAAAATCACC AGCCTTAGCT 
CCTGGGAAAC TCTCACGATT CATGAAGACT GAGAGCTCAG GGCCCCTCTT TGAATTACGA 
TCAGATCCAC ACATTCCAAA ACATTCCGCC CAACTTCCGC ACAGCTCCAG GATGCCCAGC 
AGGAGGGACT GGGTCCAGTG CCCCAAGAGT CAGACTCCAG GGTCACGGTC AAGGCCTGCC 
ATTGAGTCTA GTGACAGTGG AGAGCCCCCC ACGAGGGATG AACACTGTGG CTCTGGGCCG 
GAGGCAGGGG TGAAATCCCC TTCCCCTCCG CCCCCTCCAG GCAGGTCCGT CTCCCTGCTG 
GCCAGGCCCA GCTATGACTA TTCACCAGCA CCTTCATCCA CCAAGTCCGA AACCAGGGTC 
CCCAGTGAAA CAGCAAGGAC CCCATTCAAA TCCCCGCTGC TGAAAGGAAT TTCTGCTCCA 
GTTATTTCTT CTAATCCGGC CACGACAGAA GTGCAGAGGA AGAAACCTTC TGTGGCCTTC 
AAAAAGCCTA TCTTCACTCA CCCTATGCCC TCCCCAGAAG CAGTCATTCA AACCCGATGC 
CCTGCTCATG CCCCCTCCAG CTCCTTCACC GTAATGGCTC TGGGGCCTCC AAAGGTCTCT 
CCGAAGAGAG GTGTCCCCAA AACCTCTCCT CGCCAAACAC TTGGGACCCC ACAAAGGGAC 
ATAGGATTAC AGACTCCCAG GATCTCTCCT TCAACCCATG AGCCACTGGA AATGACGTCC 
TCCAAAAGTG TATCTCCAGG GAGAAAAGGA CAATTGAATG ATAGCGCCTC CACACCCCCC 
AAGCCTTCCT TCTTAGGGGT AAATGAGTCA CCATCATCTC AGGTCAGCAG TTCCTCATCA 
TCCTCATCAC CCGCCAAAAG CCATAACAGC CCTCATGGTT GTCAAAGTGC TCATGAGAAA 
GGACTGAAAA CTCGCCTTCC AGTGGGACTC AAAGTGCTCA TGAAGTCTCC CCAGCTGCTC 
AGGAAAAGTT CCACCGTGCC AGGGAAACAT GAAAAAGACA GTTTAAATGA AGCCTCCAAA 
AGTTCCGTGG CTGTGAACAA GTCTAAGCCA GAGGACTCCA AGAATCCAGC AAGCATGGAG 
ATCACAGCGG GTGAAAGAAA TGTGACCCTA CCGGATTCAC AAGCACAGGG CAGTTTAGCT 
GATGGGCTTC CCCTGGAAAC AGCACTACAA GAGCCATTGG AAAGTAGCAT CCCTGGGAGT 
GATGGAAGGG ATGGGGTAGA TAATAGATCC ATGAGAAGAT CCCTTTCCTC CAGCAAACCA 
CACCTAAAAC CAGCTCTGGG TATGAATGGC GCCAAAGCCC GCAGCCACAG CTTCAGTACA 
CACTCAGGAG ACAAGCCTTC TACGCCCCCC ATCGAAGGGT CAGGCAAAGT CCGCACTCAG 
ATCATTACCA ATACCGCCGA GAGAGGCAAT TCTCTTACCC GGCAGAACTC TTCCACGGAA 
AGCTCTCCCA ACAAGGCCCC TTCTGCTCCC ATGTTGGAGA GTCTCCCCAG TGTTGGGAGG 
CCCTCGGGGC ACCCCTCCTC CGGGAAGGGC TCCCTGGGGA GCTCAGGCAG CTTCAGCAGT 
CAGCATGGGA GCCCAAGTAA GTTGCCTTTG AGGATCCCTC CAAAGTCTGA GGGACTCCTC 
ATCCCACCTG GAAAGGAAGA CCAGCAGGCC TTCACCCAGG GAGAGTGCCC CAGTGCCAAT 
GTGGCTGTAC TTGGGGAACC AGGCAGTGAC CGCCGCAGTT GCCCACCCAC CCCAACAGAC 
TGCCCTGAAG CCCTGCAGAG CCCAGGGAGG ACTCAGCATC CAAGCACTTT TGAAACAAGC 
AGTACATCCA AGCTAGAAAC TTCTGGAAGG CATCCAGATG CCTCTGCAAC CGCGACTGAT 
GCTGTGAGTT CAGAAGCCCC CCTCTCACCC ACAATCGAAG AAAAGGTCAT GTTGTGCATT 
CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGCCCA CCTCTGTGGA AGCAAAGCAG 
AAGCCTGGGC CTTCTTTTGC CAGCTGGTTT GGTTTTCGGA AGAGTAGACT TCCAGCTCTG 
AGTAGCAGGA AAATGGACAT CTCCAAAACC AAAGTAGAAA AGAAAGATGC AAAAGTCTTG 
GGGTTTGGAA ACAGACAACT AAAATCAGAA AGAAAAAAAG AGAAAAAGAA GCCTGAACTA 
CAGTGTGAGA CAGAAAATGA GCTTATCAAG GACACCAAGT CAGCAGATAA TCCAGATGGC 
GGTTTACAAA GCAAAAATAA TCGGAGAACA CCACAAGACA TTTACAACCA ACTGAAGATT 
GAACCAAGGA ATAGACACAG CCCTGTTGCA TGTTCAACGA AAGACACCTT CATGACGGAA 
CTCTTGAACA GAGTTGATAA GAAAGCAGCT CCACAGACAG AAAGTGGATC AAGTAATGCT 
TCCTGCAGGA ATGTGTTAAA GGGCAGTTCT CAGGGCTCCT GTCTCATCGG CAGCTCTATC 
AGTACTCAAG GAAACCACAA GAAAAACATG AAAATCAAAG CCGATATGGA AGTACCAAAA 
GACTCCCTGG TAAAAGAGGC AAATGAAAAC TTGCAAGAGG ATGAAGACGA TGCAGTTGCA 
GATTCTGTAT TTCAGAGCCA CATCATAGAA TCCAACTGCC AGATGAGAAC ATTGGACAGT 
GGGATCGGAA CCTTTCCACT CCCAGACTCG GGAAATCGCT CGACAGGACG CTACCTATGC 
CAGCCAGACT CCCCAGAGGA CGCTGAGCCT CTCCTGCCTC TCCAGTCAGC CCTTTCTGCA 
GTTTCTTCCA TGAGAGCCCA AACCCTTGAA CGTGAAGTGC CTTCCTCCAC AGACGGCCAG 
CGCCCTGCAG ATAGCGCCAT TGTTCATTCC ACATCCGACC CCATCATGAC CGCCAGAGGG 
ATGAGGCCTC TTCAGAGCCG CCTCCCCAAA CCAGCTTCCT CAGGAAAAGT CAGTTCCCAA 
AAGCAGAATG AAGCAGAGCC AAGGCCTCAG ACATGCTCAT CATTCGGATA TGCTGAAGAC 
CCAATGGCAA GCCAGCCGCT TCCAGACTGG GGGAGTGAAG TTGCTGCCAC CGGGACCCAG 
AAATTGAGAC AACTTGAAGA AACAAAAGAC GATCCCGAGA ATAGATTATC GAAAATTTCC 
CTAGAGTCAT TCAATAAATT TAACAGCAAT ACTGTGATTT TATTAGAAAA AGAGAAGAAC 
TCTCTGAACA AGGTTGAAGG ACAGAAGGAA GAAAAAGAAA AAAATGAAGA GACATCTTTG 
AGTAGTTCAG ATAGGCCTGG GGTAGACAAC TTGGAATCTT TGAGTGATTC TTTATATGAT 
AGCTTCTCTT CCTGTGCCAG TCAAGGTTCA AATGATGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_011511104.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509406.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X13, mRNA.

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CloneID	OHu36200
Clone ID Related Accession (Same CDS sequence)	XM_011511104.1 , XM_011511105.2 , XM_011511103.2
Accession Version	XM_011511104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGAGA AGCTGATACA TGAACTGGAA GAGGAGAGAC ACTTACGTCT TCAAAGCGAG 
AAGCGGTTGC AGGAGGTGAC CCTAGAGTCT GAACGCAACA GAATTCAGAT GCGTAGCTTG 
CAGCAGCAGT TCTCCAGGAT GGAAGAAACA GTACGAAATC TATTGCAGAG TCAAGGATCT 
CCAGAGCAGA AAAAAGAAGA AACTGTTAAT ATAATGGTCT ATCAGGAAAA GCTGTCAGAG 
GAAGAGAGAA AACATAAGGA AGCTTTGGAA GATCTTCACA TGGTGGTTGA TGAGGATTCG 
AGGAGTGAAA GCAGCAGTAC AGATGAGGGA AAAGAAAAGA CCAAATTGCT ATTAGAGAGA 
TTGAAAGCTC TAGAGGCAGA GAATTCAGCG TTGGCTTTGG AGAATGAAAA TCAAAGGGAA 
CAATATGAGC GATGTCTTGA TGAGGTAGCC AACCAAGTTG TTCAAGCTCT GCTTACTCAA 
AAGGATCTAA GAGAGGAATG TGTGAAGTTG AAAACAAGAG TGTTTGATTT GGAACAGCAG 
AATCGAACAC TAAGCATCCT ATTCCAACAG CGAGTCAGAC CCACTTCTGA TCTGCTCCTC 
CAGAAACTTC ACTCACGTCT CTTGGATCTT TCATCTGGAG ATTTGCTTTC AGAGGTGGAA 
AGAAACCGAA GTCTGACACA GTCACGAACT GATGCTGAGG TGCACGAACA CCAACTAAAT 
ACAAAATCGG CCTTGAAATG CCCTGGCTTG GGGGCTGTCA TCCCTGGTCA TCTCTGTCCT 
CGAAACAGCT ACAGTAGCAG CAGTGAACTG TCTCTTTCAA GCACCTGCAG CGAGTACTCC 
AGTGGCTCCT CCTACACGTG GCACGATGGG AAGAACCTCA GGAAAAGGCA ATCATCACAA 
AACTGGGATA AAAGGCTAAG TATTGATTCT TCGCTCCCAA GTGGCTTTGC TAGTCCTACA 
AATGAACTAC CTCCAACTCG TATCAAGGAA AGCCACATTT TGGAAGGGCT AAGAAAGCTA 
CAGAAGCGAA AAGTGTTACT TGAACCCCCA TCAGTGATAA CCAAATGGGG TTATAAAGAT 
TGCATGAACT CGAATGAAGG AATATATTCT CCTGGAATTA AAAGTAGCAG CCTCAAGGAG 
TATCCCCCCT GCAAAACAGC TGACCTGGGG AGCCCCTGCA AGGAACCCCA CAAGACATTT 
GTTTATGATC TAGATTCCCA CGTTGATGCG GACGATGACC CTTCCACCTT AGCATTGCTC 
CAAGCAGTTC CAAACCAGAG CTGCAGGCCA CATGGCAGTA AATTAACCCA CAGTGTTTCC 
GACAGTCTGT TTGGCTGGGA GACAAACAGA AAACACTTTC TGGAAGGCAC ATCCTCAGTT 
TATCCCAAGG AAAGGCCTGA AAAGCTGACA AGTTGCGCCA GCAGCTGTCC CTTAGAGATG 
AAGCTGTGCC CAAGTGTGCA GACGCCTCAG GTACAGAGGG AGAGGGGCCC ACAGGGCCAA 
GGCCATGGCC GCATGGCTCT CAACCTCCAG CTTTCAGACA CTGATGACAA TGAAACGTTT 
GATGAGCTGC ACATAGAGAG CAGTGATGAG AAAAGTCCTT CAGACGTGTC ATTGGCTGCC 
GACACCGATA AGTCCGTGGA GAACCTGGAT GTCCTTGTGG GGTTTGGAAA ATCTCTATGT 
GGGTCTCCTG AAGAGGAGGA AAAACAAGTG CCCATCCCTT CAGAGACTAG GCCAAAGACT 
TTTAGTTTCA TTAAGCAGCA AAGAGTTGTA AAAAGGACTT CTTCAGAAGA ATGTGTGACT 
GTGATATTTG ATGCGGAAGA CGGTGAGCCC ATTGAATTCA GCTCTCACCA GACTGGGGTT 
GTCACTGTTA CCAGAAATGA GATTTCCATC AATTCAACTC CTGCTGGACC CAAGGCAGAA 
CATACTGAGC TTTTACCTCA GGGAATTGCT TGTTTACAGC CAAGAGCTGC TGCAAGAGAC 
TATACTTTCT TTAAAAGGTC TGAAGAGGAC ACTGAGAAAA ACATTCCAAA AGATAATGTA 
GATAATGTTC CCAGGGTGTC CACTGAATCT TTCAGCTCCA GGACAGTGAC ACAAAATCCT 
CAGCAGCAAA AGCTGGTCAA ACCAACACAC AATATATCAT GCCAGAGTAA TTCCAGGTCT 
TCGGCACCCA TGGGCATCTA TCAAAAGCAA AATCTGACAA AAATACCTCC CAGGGGCAAG 
TCTTCACCTC AGAAATCAAA ACTAATGGAG CCCGAAGCCA CCACACTACT CCCTTCATCT 
GGCCTGGTGA CTCTTGAAAA ATCACCAGCC TTAGCTCCTG GGAAACTCTC ACGATTCATG 
AAGACTGAGA GCTCAGGGCC CCTCTTTGAA TTACGATCAG ATCCACACAT TCCAAAACAT 
TCCGCCCAAC TTCCGCACAG CTCCAGGATG CCCAGCAGGA GGGACTGGGT CCAGTGCCCC 
AAGAGTCAGA CTCCAGGGTC ACGGTCAAGG CCTGCCATTG AGTCTAGTGA CAGTGGAGAG 
CCCCCCACGA GGGATGAACA CTGTGGCTCT GGGCCGGAGG CAGGGGTGAA ATCCCCTTCC 
CCTCCGCCCC CTCCAGGCAG GTCCGTCTCC CTGCTGGCCA GGCCCAGCTA TGACTATTCA 
CCAGCACCTT CATCCACCAA GTCCGAAACC AGGGTCCCCA GTGAAACAGC AAGGACCCCA 
TTCAAATCCC CGCTGCTGAA AGGAATTTCT GCTCCAGTTA TTTCTTCTAA TCCGGCCACG 
ACAGAAGTGC AGAGGAAGAA ACCTTCTGTG GCCTTCAAAA AGCCTATCTT CACTCACCCT 
ATGCCCTCCC CAGAAGCAGT CATTCAAACC CGATGCCCTG CTCATGCCCC CTCCAGCTCC 
TTCACCGTAA TGGCTCTGGG GCCTCCAAAG GTCTCTCCGA AGAGAGGTGT CCCCAAAACC 
TCTCCTCGCC AAACACTTGG GACCCCACAA AGGGACATAG GATTACAGAC TCCCAGGATC 
TCTCCTTCAA CCCATGAGCC ACTGGAAATG ACGTCCTCCA AAAGTGTATC TCCAGGGAGA 
AAAGGACAAT TGAATGATAG CGCCTCCACA CCCCCCAAGC CTTCCTTCTT AGGGGTAAAT 
GAGTCACCAT CATCTCAGGT CAGCAGTTCC TCATCATCCT CATCACCCGC CAAAAGCCAT 
AACAGCCCTC ATGGTTGTCA AAGTGCTCAT GAGAAAGGAC TGAAAACTCG CCTTCCAGTG 
GGACTCAAAG TGCTCATGAA GTCTCCCCAG CTGCTCAGGA AAAGTTCCAC CGTGCCAGGG 
AAACATGAAA AAGACAGTTT AAATGAAGCC TCCAAAAGTT CCGTGGCTGT GAACAAGTCT 
AAGCCAGAGG ACTCCAAGAA TCCAGCAAGC ATGGAGATCA CAGCGGGTGA AAGAAATGTG 
ACCCTACCGG ATTCACAAGC ACAGGGCAGT TTAGCTGATG GGCTTCCCCT GGAAACAGCA 
CTACAAGAGC CATTGGAAAG TAGCATCCCT GGGAGTGATG GAAGGGATGG GGTAGATAAT 
AGATCCATGA GAAGATCCCT TTCCTCCAGC AAACCACACC TAAAACCAGC TCTGGGTATG 
AATGGCGCCA AAGCCCGCAG CCACAGCTTC AGTACACACT CAGGAGACAA GCCTTCTACG 
CCCCCCATCG AAGGGTCAGG CAAAGTCCGC ACTCAGATCA TTACCAATAC CGCCGAGAGA 
GGCAATTCTC TTACCCGGCA GAACTCTTCC ACGGAAAGCT CTCCCAACAA GGCCCCTTCT 
GCTCCCATGT TGGAGAGTCT CCCCAGTGTT GGGAGGCCCT CGGGGCACCC CTCCTCCGGG 
AAGGGCTCCC TGGGGAGCTC AGGCAGCTTC AGCAGTCAGC ATGGGAGCCC AAGTAAGTTG 
CCTTTGAGGA TCCCTCCAAA GTCTGAGGGA CTCCTCATCC CACCTGGAAA GGAAGACCAG 
CAGGCCTTCA CCCAGGGAGA GTGCCCCAGT GCCAATGTGG CTGTACTTGG GGAACCAGGC 
AGTGACCGCC GCAGTTGCCC ACCCACCCCA ACAGACTGCC CTGAAGCCCT GCAGAGCCCA 
GGGAGGACTC AGCATCCAAG CACTTTTGAA ACAAGCAGTA CATCCAAGCT AGAAACTTCT 
GGAAGGCATC CAGATGCCTC TGCAACCGCG ACTGATGCTG TGAGTTCAGA AGCCCCCCTC 
TCACCCACAA TCGAAGAAAA GGTCATGTTG TGCATTCAGG AAAATGTGGA AAAGGGCCAA 
GTGCAAACAA AGCCCACCTC TGTGGAAGCA AAGCAGAAGC CTGGGCCTTC TTTTGCCAGC 
TGGTTTGGTT TTCGGAAGAG TAGACTTCCA GCTCTGAGTA GCAGGAAAAT GGACATCTCC 
AAAACCAAAG TAGAAAAGAA AGATGCAAAA GTCTTGGGGT TTGGAAACAG ACAACTAAAA 
TCAGAAAGAA AAAAAGAGAA AAAGAAGCCT GAACTACAGT GTGAGACAGA AAATGAGCTT 
ATCAAGGACA CCAAGTCAGC AGATAATCCA GATGGCGGTT TACAAAGCAA AAATAATCGG 
AGAACACCAC AAGACATTTA CAACCAACTG AAGATTGAAC CAAGGAATAG ACACAGCCCT 
GTTGCATGTT CAACGAAAGA CACCTTCATG ACGGAACTCT TGAACAGAGT TGATAAGAAA 
GCAGCTCCAC AGACAGAAAG TGGATCAAGT AATGCTTCCT GCAGGAATGT GTTAAAGGGC 
AGTTCTCAGG GCTCCTGTCT CATCGGCAGC TCTATCAGTA CTCAAGGAAA CCACAAGAAA 
AACATGAAAA TCAAAGCCGA TATGGAAGTA CCAAAAGACT CCCTGGTAAA AGAGGCAAAT 
GAAAACTTGC AAGAGGATGA AGACGATGCA GTTGCAGATT CTGTATTTCA GAGCCACATC 
ATAGAATCCA ACTGCCAGAT GAGAACATTG GACAGTGGGA TCGGAACCTT TCCACTCCCA 
GACTCGGGAA ATCGCTCGAC AGGACGCTAC CTATGCCAGC CAGACTCCCC AGAGGACGCT 
GAGCCTCTCC TGCCTCTCCA GTCAGCCCTT TCTGCAGTTT CTTCCATGAG AGCCCAAACC 
CTTGAACGTG AAGTGCCTTC CTCCACAGAC GGCCAGCGCC CTGCAGATAG CGCCATTGTT 
CATTCCACAT CCGACCCCAT CATGACCGCC AGAGGGATGA GGCCTCTTCA GAGCCGCCTC 
CCCAAACCAG CTTCCTCAGG AAAAGTCAGT TCCCAAAAGC AGAATGAAGC AGAGCCAAGG 
CCTCAGACAT GCTCATCATT CGGATATGCT GAAGACCCAA TGGCAAGCCA GCCGCTTCCA 
GACTGGGGGA GTGAAGTTGC TGCCACCGGG ACCCAGAAAT TGAGACAACT TGAAGAAACA 
AAAGACGATC CCGAGAATAG ATTATCGAAA ATTTCCCTAG AGTCATTCAA TAAATTTAAC 
AGCAATACTG TGATTTTATT AGAAAAAGAG AAGAACTCTC TGAACAAGGT TGAAGGACAG 
AAGGAAGAAA AAGAAAAAAA TGAAGAGACA TCTTTGAGTA GTTCAGATAG GCCTGGGGTA 
GACAACTTGG AATCTTTGAG TGATTCTTTA TATGATAGCT TCTCTTCCTG TGCCAGTCAA 
GGTTCAAATG ATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509406.1
CDS	131..5788
Translation	MHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_011511104.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511104.1

ORF Insert Sequence:

ATGCATGAGA AGCTGATACA TGAACTGGAA GAGGAGAGAC ACTTACGTCT TCAAAGCGAG 
AAGCGGTTGC AGGAGGTGAC CCTAGAGTCT GAACGCAACA GAATTCAGAT GCGTAGCTTG 
CAGCAGCAGT TCTCCAGGAT GGAAGAAACA GTACGAAATC TATTGCAGAG TCAAGGATCT 
CCAGAGCAGA AAAAAGAAGA AACTGTTAAT ATAATGGTCT ATCAGGAAAA GCTGTCAGAG 
GAAGAGAGAA AACATAAGGA AGCTTTGGAA GATCTTCACA TGGTGGTTGA TGAGGATTCG 
AGGAGTGAAA GCAGCAGTAC AGATGAGGGA AAAGAAAAGA CCAAATTGCT ATTAGAGAGA 
TTGAAAGCTC TAGAGGCAGA GAATTCAGCG TTGGCTTTGG AGAATGAAAA TCAAAGGGAA 
CAATATGAGC GATGTCTTGA TGAGGTAGCC AACCAAGTTG TTCAAGCTCT GCTTACTCAA 
AAGGATCTAA GAGAGGAATG TGTGAAGTTG AAAACAAGAG TGTTTGATTT GGAACAGCAG 
AATCGAACAC TAAGCATCCT ATTCCAACAG CGAGTCAGAC CCACTTCTGA TCTGCTCCTC 
CAGAAACTTC ACTCACGTCT CTTGGATCTT TCATCTGGAG ATTTGCTTTC AGAGGTGGAA 
AGAAACCGAA GTCTGACACA GTCACGAACT GATGCTGAGG TGCACGAACA CCAACTAAAT 
ACAAAATCGG CCTTGAAATG CCCTGGCTTG GGGGCTGTCA TCCCTGGTCA TCTCTGTCCT 
CGAAACAGCT ACAGTAGCAG CAGTGAACTG TCTCTTTCAA GCACCTGCAG CGAGTACTCC 
AGTGGCTCCT CCTACACGTG GCACGATGGG AAGAACCTCA GGAAAAGGCA ATCATCACAA 
AACTGGGATA AAAGGCTAAG TATTGATTCT TCGCTCCCAA GTGGCTTTGC TAGTCCTACA 
AATGAACTAC CTCCAACTCG TATCAAGGAA AGCCACATTT TGGAAGGGCT AAGAAAGCTA 
CAGAAGCGAA AAGTGTTACT TGAACCCCCA TCAGTGATAA CCAAATGGGG TTATAAAGAT 
TGCATGAACT CGAATGAAGG AATATATTCT CCTGGAATTA AAAGTAGCAG CCTCAAGGAG 
TATCCCCCCT GCAAAACAGC TGACCTGGGG AGCCCCTGCA AGGAACCCCA CAAGACATTT 
GTTTATGATC TAGATTCCCA CGTTGATGCG GACGATGACC CTTCCACCTT AGCATTGCTC 
CAAGCAGTTC CAAACCAGAG CTGCAGGCCA CATGGCAGTA AATTAACCCA CAGTGTTTCC 
GACAGTCTGT TTGGCTGGGA GACAAACAGA AAACACTTTC TGGAAGGCAC ATCCTCAGTT 
TATCCCAAGG AAAGGCCTGA AAAGCTGACA AGTTGCGCCA GCAGCTGTCC CTTAGAGATG 
AAGCTGTGCC CAAGTGTGCA GACGCCTCAG GTACAGAGGG AGAGGGGCCC ACAGGGCCAA 
GGCCATGGCC GCATGGCTCT CAACCTCCAG CTTTCAGACA CTGATGACAA TGAAACGTTT 
GATGAGCTGC ACATAGAGAG CAGTGATGAG AAAAGTCCTT CAGACGTGTC ATTGGCTGCC 
GACACCGATA AGTCCGTGGA GAACCTGGAT GTCCTTGTGG GGTTTGGAAA ATCTCTATGT 
GGGTCTCCTG AAGAGGAGGA AAAACAAGTG CCCATCCCTT CAGAGACTAG GCCAAAGACT 
TTTAGTTTCA TTAAGCAGCA AAGAGTTGTA AAAAGGACTT CTTCAGAAGA ATGTGTGACT 
GTGATATTTG ATGCGGAAGA CGGTGAGCCC ATTGAATTCA GCTCTCACCA GACTGGGGTT 
GTCACTGTTA CCAGAAATGA GATTTCCATC AATTCAACTC CTGCTGGACC CAAGGCAGAA 
CATACTGAGC TTTTACCTCA GGGAATTGCT TGTTTACAGC CAAGAGCTGC TGCAAGAGAC 
TATACTTTCT TTAAAAGGTC TGAAGAGGAC ACTGAGAAAA ACATTCCAAA AGATAATGTA 
GATAATGTTC CCAGGGTGTC CACTGAATCT TTCAGCTCCA GGACAGTGAC ACAAAATCCT 
CAGCAGCAAA AGCTGGTCAA ACCAACACAC AATATATCAT GCCAGAGTAA TTCCAGGTCT 
TCGGCACCCA TGGGCATCTA TCAAAAGCAA AATCTGACAA AAATACCTCC CAGGGGCAAG 
TCTTCACCTC AGAAATCAAA ACTAATGGAG CCCGAAGCCA CCACACTACT CCCTTCATCT 
GGCCTGGTGA CTCTTGAAAA ATCACCAGCC TTAGCTCCTG GGAAACTCTC ACGATTCATG 
AAGACTGAGA GCTCAGGGCC CCTCTTTGAA TTACGATCAG ATCCACACAT TCCAAAACAT 
TCCGCCCAAC TTCCGCACAG CTCCAGGATG CCCAGCAGGA GGGACTGGGT CCAGTGCCCC 
AAGAGTCAGA CTCCAGGGTC ACGGTCAAGG CCTGCCATTG AGTCTAGTGA CAGTGGAGAG 
CCCCCCACGA GGGATGAACA CTGTGGCTCT GGGCCGGAGG CAGGGGTGAA ATCCCCTTCC 
CCTCCGCCCC CTCCAGGCAG GTCCGTCTCC CTGCTGGCCA GGCCCAGCTA TGACTATTCA 
CCAGCACCTT CATCCACCAA GTCCGAAACC AGGGTCCCCA GTGAAACAGC AAGGACCCCA 
TTCAAATCCC CGCTGCTGAA AGGAATTTCT GCTCCAGTTA TTTCTTCTAA TCCGGCCACG 
ACAGAAGTGC AGAGGAAGAA ACCTTCTGTG GCCTTCAAAA AGCCTATCTT CACTCACCCT 
ATGCCCTCCC CAGAAGCAGT CATTCAAACC CGATGCCCTG CTCATGCCCC CTCCAGCTCC 
TTCACCGTAA TGGCTCTGGG GCCTCCAAAG GTCTCTCCGA AGAGAGGTGT CCCCAAAACC 
TCTCCTCGCC AAACACTTGG GACCCCACAA AGGGACATAG GATTACAGAC TCCCAGGATC 
TCTCCTTCAA CCCATGAGCC ACTGGAAATG ACGTCCTCCA AAAGTGTATC TCCAGGGAGA 
AAAGGACAAT TGAATGATAG CGCCTCCACA CCCCCCAAGC CTTCCTTCTT AGGGGTAAAT 
GAGTCACCAT CATCTCAGGT CAGCAGTTCC TCATCATCCT CATCACCCGC CAAAAGCCAT 
AACAGCCCTC ATGGTTGTCA AAGTGCTCAT GAGAAAGGAC TGAAAACTCG CCTTCCAGTG 
GGACTCAAAG TGCTCATGAA GTCTCCCCAG CTGCTCAGGA AAAGTTCCAC CGTGCCAGGG 
AAACATGAAA AAGACAGTTT AAATGAAGCC TCCAAAAGTT CCGTGGCTGT GAACAAGTCT 
AAGCCAGAGG ACTCCAAGAA TCCAGCAAGC ATGGAGATCA CAGCGGGTGA AAGAAATGTG 
ACCCTACCGG ATTCACAAGC ACAGGGCAGT TTAGCTGATG GGCTTCCCCT GGAAACAGCA 
CTACAAGAGC CATTGGAAAG TAGCATCCCT GGGAGTGATG GAAGGGATGG GGTAGATAAT 
AGATCCATGA GAAGATCCCT TTCCTCCAGC AAACCACACC TAAAACCAGC TCTGGGTATG 
AATGGCGCCA AAGCCCGCAG CCACAGCTTC AGTACACACT CAGGAGACAA GCCTTCTACG 
CCCCCCATCG AAGGGTCAGG CAAAGTCCGC ACTCAGATCA TTACCAATAC CGCCGAGAGA 
GGCAATTCTC TTACCCGGCA GAACTCTTCC ACGGAAAGCT CTCCCAACAA GGCCCCTTCT 
GCTCCCATGT TGGAGAGTCT CCCCAGTGTT GGGAGGCCCT CGGGGCACCC CTCCTCCGGG 
AAGGGCTCCC TGGGGAGCTC AGGCAGCTTC AGCAGTCAGC ATGGGAGCCC AAGTAAGTTG 
CCTTTGAGGA TCCCTCCAAA GTCTGAGGGA CTCCTCATCC CACCTGGAAA GGAAGACCAG 
CAGGCCTTCA CCCAGGGAGA GTGCCCCAGT GCCAATGTGG CTGTACTTGG GGAACCAGGC 
AGTGACCGCC GCAGTTGCCC ACCCACCCCA ACAGACTGCC CTGAAGCCCT GCAGAGCCCA 
GGGAGGACTC AGCATCCAAG CACTTTTGAA ACAAGCAGTA CATCCAAGCT AGAAACTTCT 
GGAAGGCATC CAGATGCCTC TGCAACCGCG ACTGATGCTG TGAGTTCAGA AGCCCCCCTC 
TCACCCACAA TCGAAGAAAA GGTCATGTTG TGCATTCAGG AAAATGTGGA AAAGGGCCAA 
GTGCAAACAA AGCCCACCTC TGTGGAAGCA AAGCAGAAGC CTGGGCCTTC TTTTGCCAGC 
TGGTTTGGTT TTCGGAAGAG TAGACTTCCA GCTCTGAGTA GCAGGAAAAT GGACATCTCC 
AAAACCAAAG TAGAAAAGAA AGATGCAAAA GTCTTGGGGT TTGGAAACAG ACAACTAAAA 
TCAGAAAGAA AAAAAGAGAA AAAGAAGCCT GAACTACAGT GTGAGACAGA AAATGAGCTT 
ATCAAGGACA CCAAGTCAGC AGATAATCCA GATGGCGGTT TACAAAGCAA AAATAATCGG 
AGAACACCAC AAGACATTTA CAACCAACTG AAGATTGAAC CAAGGAATAG ACACAGCCCT 
GTTGCATGTT CAACGAAAGA CACCTTCATG ACGGAACTCT TGAACAGAGT TGATAAGAAA 
GCAGCTCCAC AGACAGAAAG TGGATCAAGT AATGCTTCCT GCAGGAATGT GTTAAAGGGC 
AGTTCTCAGG GCTCCTGTCT CATCGGCAGC TCTATCAGTA CTCAAGGAAA CCACAAGAAA 
AACATGAAAA TCAAAGCCGA TATGGAAGTA CCAAAAGACT CCCTGGTAAA AGAGGCAAAT 
GAAAACTTGC AAGAGGATGA AGACGATGCA GTTGCAGATT CTGTATTTCA GAGCCACATC 
ATAGAATCCA ACTGCCAGAT GAGAACATTG GACAGTGGGA TCGGAACCTT TCCACTCCCA 
GACTCGGGAA ATCGCTCGAC AGGACGCTAC CTATGCCAGC CAGACTCCCC AGAGGACGCT 
GAGCCTCTCC TGCCTCTCCA GTCAGCCCTT TCTGCAGTTT CTTCCATGAG AGCCCAAACC 
CTTGAACGTG AAGTGCCTTC CTCCACAGAC GGCCAGCGCC CTGCAGATAG CGCCATTGTT 
CATTCCACAT CCGACCCCAT CATGACCGCC AGAGGGATGA GGCCTCTTCA GAGCCGCCTC 
CCCAAACCAG CTTCCTCAGG AAAAGTCAGT TCCCAAAAGC AGAATGAAGC AGAGCCAAGG 
CCTCAGACAT GCTCATCATT CGGATATGCT GAAGACCCAA TGGCAAGCCA GCCGCTTCCA 
GACTGGGGGA GTGAAGTTGC TGCCACCGGG ACCCAGAAAT TGAGACAACT TGAAGAAACA 
AAAGACGATC CCGAGAATAG ATTATCGAAA ATTTCCCTAG AGTCATTCAA TAAATTTAAC 
AGCAATACTG TGATTTTATT AGAAAAAGAG AAGAACTCTC TGAACAAGGT TGAAGGACAG 
AAGGAAGAAA AAGAAAAAAA TGAAGAGACA TCTTTGAGTA GTTCAGATAG GCCTGGGGTA 
GACAACTTGG AATCTTTGAG TGATTCTTTA TATGATAGCT TCTCTTCCTG TGCCAGTCAA 
GGTTCAAATG ATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_011511105.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509407.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X14, mRNA.

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CloneID	OHu36200
Clone ID Related Accession (Same CDS sequence)	XM_011511104.1 , XM_011511105.2 , XM_011511103.2
Accession Version	XM_011511105.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511105.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGAGA AGCTGATACA TGAACTGGAA GAGGAGAGAC ACTTACGTCT TCAAAGCGAG 
AAGCGGTTGC AGGAGGTGAC CCTAGAGTCT GAACGCAACA GAATTCAGAT GCGTAGCTTG 
CAGCAGCAGT TCTCCAGGAT GGAAGAAACA GTACGAAATC TATTGCAGAG TCAAGGATCT 
CCAGAGCAGA AAAAAGAAGA AACTGTTAAT ATAATGGTCT ATCAGGAAAA GCTGTCAGAG 
GAAGAGAGAA AACATAAGGA AGCTTTGGAA GATCTTCACA TGGTGGTTGA TGAGGATTCG 
AGGAGTGAAA GCAGCAGTAC AGATGAGGGA AAAGAAAAGA CCAAATTGCT ATTAGAGAGA 
TTGAAAGCTC TAGAGGCAGA GAATTCAGCG TTGGCTTTGG AGAATGAAAA TCAAAGGGAA 
CAATATGAGC GATGTCTTGA TGAGGTAGCC AACCAAGTTG TTCAAGCTCT GCTTACTCAA 
AAGGATCTAA GAGAGGAATG TGTGAAGTTG AAAACAAGAG TGTTTGATTT GGAACAGCAG 
AATCGAACAC TAAGCATCCT ATTCCAACAG CGAGTCAGAC CCACTTCTGA TCTGCTCCTC 
CAGAAACTTC ACTCACGTCT CTTGGATCTT TCATCTGGAG ATTTGCTTTC AGAGGTGGAA 
AGAAACCGAA GTCTGACACA GTCACGAACT GATGCTGAGG TGCACGAACA CCAACTAAAT 
ACAAAATCGG CCTTGAAATG CCCTGGCTTG GGGGCTGTCA TCCCTGGTCA TCTCTGTCCT 
CGAAACAGCT ACAGTAGCAG CAGTGAACTG TCTCTTTCAA GCACCTGCAG CGAGTACTCC 
AGTGGCTCCT CCTACACGTG GCACGATGGG AAGAACCTCA GGAAAAGGCA ATCATCACAA 
AACTGGGATA AAAGGCTAAG TATTGATTCT TCGCTCCCAA GTGGCTTTGC TAGTCCTACA 
AATGAACTAC CTCCAACTCG TATCAAGGAA AGCCACATTT TGGAAGGGCT AAGAAAGCTA 
CAGAAGCGAA AAGTGTTACT TGAACCCCCA TCAGTGATAA CCAAATGGGG TTATAAAGAT 
TGCATGAACT CGAATGAAGG AATATATTCT CCTGGAATTA AAAGTAGCAG CCTCAAGGAG 
TATCCCCCCT GCAAAACAGC TGACCTGGGG AGCCCCTGCA AGGAACCCCA CAAGACATTT 
GTTTATGATC TAGATTCCCA CGTTGATGCG GACGATGACC CTTCCACCTT AGCATTGCTC 
CAAGCAGTTC CAAACCAGAG CTGCAGGCCA CATGGCAGTA AATTAACCCA CAGTGTTTCC 
GACAGTCTGT TTGGCTGGGA GACAAACAGA AAACACTTTC TGGAAGGCAC ATCCTCAGTT 
TATCCCAAGG AAAGGCCTGA AAAGCTGACA AGTTGCGCCA GCAGCTGTCC CTTAGAGATG 
AAGCTGTGCC CAAGTGTGCA GACGCCTCAG GTACAGAGGG AGAGGGGCCC ACAGGGCCAA 
GGCCATGGCC GCATGGCTCT CAACCTCCAG CTTTCAGACA CTGATGACAA TGAAACGTTT 
GATGAGCTGC ACATAGAGAG CAGTGATGAG AAAAGTCCTT CAGACGTGTC ATTGGCTGCC 
GACACCGATA AGTCCGTGGA GAACCTGGAT GTCCTTGTGG GGTTTGGAAA ATCTCTATGT 
GGGTCTCCTG AAGAGGAGGA AAAACAAGTG CCCATCCCTT CAGAGACTAG GCCAAAGACT 
TTTAGTTTCA TTAAGCAGCA AAGAGTTGTA AAAAGGACTT CTTCAGAAGA ATGTGTGACT 
GTGATATTTG ATGCGGAAGA CGGTGAGCCC ATTGAATTCA GCTCTCACCA GACTGGGGTT 
GTCACTGTTA CCAGAAATGA GATTTCCATC AATTCAACTC CTGCTGGACC CAAGGCAGAA 
CATACTGAGC TTTTACCTCA GGGAATTGCT TGTTTACAGC CAAGAGCTGC TGCAAGAGAC 
TATACTTTCT TTAAAAGGTC TGAAGAGGAC ACTGAGAAAA ACATTCCAAA AGATAATGTA 
GATAATGTTC CCAGGGTGTC CACTGAATCT TTCAGCTCCA GGACAGTGAC ACAAAATCCT 
CAGCAGCAAA AGCTGGTCAA ACCAACACAC AATATATCAT GCCAGAGTAA TTCCAGGTCT 
TCGGCACCCA TGGGCATCTA TCAAAAGCAA AATCTGACAA AAATACCTCC CAGGGGCAAG 
TCTTCACCTC AGAAATCAAA ACTAATGGAG CCCGAAGCCA CCACACTACT CCCTTCATCT 
GGCCTGGTGA CTCTTGAAAA ATCACCAGCC TTAGCTCCTG GGAAACTCTC ACGATTCATG 
AAGACTGAGA GCTCAGGGCC CCTCTTTGAA TTACGATCAG ATCCACACAT TCCAAAACAT 
TCCGCCCAAC TTCCGCACAG CTCCAGGATG CCCAGCAGGA GGGACTGGGT CCAGTGCCCC 
AAGAGTCAGA CTCCAGGGTC ACGGTCAAGG CCTGCCATTG AGTCTAGTGA CAGTGGAGAG 
CCCCCCACGA GGGATGAACA CTGTGGCTCT GGGCCGGAGG CAGGGGTGAA ATCCCCTTCC 
CCTCCGCCCC CTCCAGGCAG GTCCGTCTCC CTGCTGGCCA GGCCCAGCTA TGACTATTCA 
CCAGCACCTT CATCCACCAA GTCCGAAACC AGGGTCCCCA GTGAAACAGC AAGGACCCCA 
TTCAAATCCC CGCTGCTGAA AGGAATTTCT GCTCCAGTTA TTTCTTCTAA TCCGGCCACG 
ACAGAAGTGC AGAGGAAGAA ACCTTCTGTG GCCTTCAAAA AGCCTATCTT CACTCACCCT 
ATGCCCTCCC CAGAAGCAGT CATTCAAACC CGATGCCCTG CTCATGCCCC CTCCAGCTCC 
TTCACCGTAA TGGCTCTGGG GCCTCCAAAG GTCTCTCCGA AGAGAGGTGT CCCCAAAACC 
TCTCCTCGCC AAACACTTGG GACCCCACAA AGGGACATAG GATTACAGAC TCCCAGGATC 
TCTCCTTCAA CCCATGAGCC ACTGGAAATG ACGTCCTCCA AAAGTGTATC TCCAGGGAGA 
AAAGGACAAT TGAATGATAG CGCCTCCACA CCCCCCAAGC CTTCCTTCTT AGGGGTAAAT 
GAGTCACCAT CATCTCAGGT CAGCAGTTCC TCATCATCCT CATCACCCGC CAAAAGCCAT 
AACAGCCCTC ATGGTTGTCA AAGTGCTCAT GAGAAAGGAC TGAAAACTCG CCTTCCAGTG 
GGACTCAAAG TGCTCATGAA GTCTCCCCAG CTGCTCAGGA AAAGTTCCAC CGTGCCAGGG 
AAACATGAAA AAGACAGTTT AAATGAAGCC TCCAAAAGTT CCGTGGCTGT GAACAAGTCT 
AAGCCAGAGG ACTCCAAGAA TCCAGCAAGC ATGGAGATCA CAGCGGGTGA AAGAAATGTG 
ACCCTACCGG ATTCACAAGC ACAGGGCAGT TTAGCTGATG GGCTTCCCCT GGAAACAGCA 
CTACAAGAGC CATTGGAAAG TAGCATCCCT GGGAGTGATG GAAGGGATGG GGTAGATAAT 
AGATCCATGA GAAGATCCCT TTCCTCCAGC AAACCACACC TAAAACCAGC TCTGGGTATG 
AATGGCGCCA AAGCCCGCAG CCACAGCTTC AGTACACACT CAGGAGACAA GCCTTCTACG 
CCCCCCATCG AAGGGTCAGG CAAAGTCCGC ACTCAGATCA TTACCAATAC CGCCGAGAGA 
GGCAATTCTC TTACCCGGCA GAACTCTTCC ACGGAAAGCT CTCCCAACAA GGCCCCTTCT 
GCTCCCATGT TGGAGAGTCT CCCCAGTGTT GGGAGGCCCT CGGGGCACCC CTCCTCCGGG 
AAGGGCTCCC TGGGGAGCTC AGGCAGCTTC AGCAGTCAGC ATGGGAGCCC AAGTAAGTTG 
CCTTTGAGGA TCCCTCCAAA GTCTGAGGGA CTCCTCATCC CACCTGGAAA GGAAGACCAG 
CAGGCCTTCA CCCAGGGAGA GTGCCCCAGT GCCAATGTGG CTGTACTTGG GGAACCAGGC 
AGTGACCGCC GCAGTTGCCC ACCCACCCCA ACAGACTGCC CTGAAGCCCT GCAGAGCCCA 
GGGAGGACTC AGCATCCAAG CACTTTTGAA ACAAGCAGTA CATCCAAGCT AGAAACTTCT 
GGAAGGCATC CAGATGCCTC TGCAACCGCG ACTGATGCTG TGAGTTCAGA AGCCCCCCTC 
TCACCCACAA TCGAAGAAAA GGTCATGTTG TGCATTCAGG AAAATGTGGA AAAGGGCCAA 
GTGCAAACAA AGCCCACCTC TGTGGAAGCA AAGCAGAAGC CTGGGCCTTC TTTTGCCAGC 
TGGTTTGGTT TTCGGAAGAG TAGACTTCCA GCTCTGAGTA GCAGGAAAAT GGACATCTCC 
AAAACCAAAG TAGAAAAGAA AGATGCAAAA GTCTTGGGGT TTGGAAACAG ACAACTAAAA 
TCAGAAAGAA AAAAAGAGAA AAAGAAGCCT GAACTACAGT GTGAGACAGA AAATGAGCTT 
ATCAAGGACA CCAAGTCAGC AGATAATCCA GATGGCGGTT TACAAAGCAA AAATAATCGG 
AGAACACCAC AAGACATTTA CAACCAACTG AAGATTGAAC CAAGGAATAG ACACAGCCCT 
GTTGCATGTT CAACGAAAGA CACCTTCATG ACGGAACTCT TGAACAGAGT TGATAAGAAA 
GCAGCTCCAC AGACAGAAAG TGGATCAAGT AATGCTTCCT GCAGGAATGT GTTAAAGGGC 
AGTTCTCAGG GCTCCTGTCT CATCGGCAGC TCTATCAGTA CTCAAGGAAA CCACAAGAAA 
AACATGAAAA TCAAAGCCGA TATGGAAGTA CCAAAAGACT CCCTGGTAAA AGAGGCAAAT 
GAAAACTTGC AAGAGGATGA AGACGATGCA GTTGCAGATT CTGTATTTCA GAGCCACATC 
ATAGAATCCA ACTGCCAGAT GAGAACATTG GACAGTGGGA TCGGAACCTT TCCACTCCCA 
GACTCGGGAA ATCGCTCGAC AGGACGCTAC CTATGCCAGC CAGACTCCCC AGAGGACGCT 
GAGCCTCTCC TGCCTCTCCA GTCAGCCCTT TCTGCAGTTT CTTCCATGAG AGCCCAAACC 
CTTGAACGTG AAGTGCCTTC CTCCACAGAC GGCCAGCGCC CTGCAGATAG CGCCATTGTT 
CATTCCACAT CCGACCCCAT CATGACCGCC AGAGGGATGA GGCCTCTTCA GAGCCGCCTC 
CCCAAACCAG CTTCCTCAGG AAAAGTCAGT TCCCAAAAGC AGAATGAAGC AGAGCCAAGG 
CCTCAGACAT GCTCATCATT CGGATATGCT GAAGACCCAA TGGCAAGCCA GCCGCTTCCA 
GACTGGGGGA GTGAAGTTGC TGCCACCGGG ACCCAGAAAT TGAGACAACT TGAAGAAACA 
AAAGACGATC CCGAGAATAG ATTATCGAAA ATTTCCCTAG AGTCATTCAA TAAATTTAAC 
AGCAATACTG TGATTTTATT AGAAAAAGAG AAGAACTCTC TGAACAAGGT TGAAGGACAG 
AAGGAAGAAA AAGAAAAAAA TGAAGAGACA TCTTTGAGTA GTTCAGATAG GCCTGGGGTA 
GACAACTTGG AATCTTTGAG TGATTCTTTA TATGATAGCT TCTCTTCCTG TGCCAGTCAA 
GGTTCAAATG ATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509407.1
CDS	922..6579
Translation	MHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_011511105.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511105.2

ORF Insert Sequence:

ATGCATGAGA AGCTGATACA TGAACTGGAA GAGGAGAGAC ACTTACGTCT TCAAAGCGAG 
AAGCGGTTGC AGGAGGTGAC CCTAGAGTCT GAACGCAACA GAATTCAGAT GCGTAGCTTG 
CAGCAGCAGT TCTCCAGGAT GGAAGAAACA GTACGAAATC TATTGCAGAG TCAAGGATCT 
CCAGAGCAGA AAAAAGAAGA AACTGTTAAT ATAATGGTCT ATCAGGAAAA GCTGTCAGAG 
GAAGAGAGAA AACATAAGGA AGCTTTGGAA GATCTTCACA TGGTGGTTGA TGAGGATTCG 
AGGAGTGAAA GCAGCAGTAC AGATGAGGGA AAAGAAAAGA CCAAATTGCT ATTAGAGAGA 
TTGAAAGCTC TAGAGGCAGA GAATTCAGCG TTGGCTTTGG AGAATGAAAA TCAAAGGGAA 
CAATATGAGC GATGTCTTGA TGAGGTAGCC AACCAAGTTG TTCAAGCTCT GCTTACTCAA 
AAGGATCTAA GAGAGGAATG TGTGAAGTTG AAAACAAGAG TGTTTGATTT GGAACAGCAG 
AATCGAACAC TAAGCATCCT ATTCCAACAG CGAGTCAGAC CCACTTCTGA TCTGCTCCTC 
CAGAAACTTC ACTCACGTCT CTTGGATCTT TCATCTGGAG ATTTGCTTTC AGAGGTGGAA 
AGAAACCGAA GTCTGACACA GTCACGAACT GATGCTGAGG TGCACGAACA CCAACTAAAT 
ACAAAATCGG CCTTGAAATG CCCTGGCTTG GGGGCTGTCA TCCCTGGTCA TCTCTGTCCT 
CGAAACAGCT ACAGTAGCAG CAGTGAACTG TCTCTTTCAA GCACCTGCAG CGAGTACTCC 
AGTGGCTCCT CCTACACGTG GCACGATGGG AAGAACCTCA GGAAAAGGCA ATCATCACAA 
AACTGGGATA AAAGGCTAAG TATTGATTCT TCGCTCCCAA GTGGCTTTGC TAGTCCTACA 
AATGAACTAC CTCCAACTCG TATCAAGGAA AGCCACATTT TGGAAGGGCT AAGAAAGCTA 
CAGAAGCGAA AAGTGTTACT TGAACCCCCA TCAGTGATAA CCAAATGGGG TTATAAAGAT 
TGCATGAACT CGAATGAAGG AATATATTCT CCTGGAATTA AAAGTAGCAG CCTCAAGGAG 
TATCCCCCCT GCAAAACAGC TGACCTGGGG AGCCCCTGCA AGGAACCCCA CAAGACATTT 
GTTTATGATC TAGATTCCCA CGTTGATGCG GACGATGACC CTTCCACCTT AGCATTGCTC 
CAAGCAGTTC CAAACCAGAG CTGCAGGCCA CATGGCAGTA AATTAACCCA CAGTGTTTCC 
GACAGTCTGT TTGGCTGGGA GACAAACAGA AAACACTTTC TGGAAGGCAC ATCCTCAGTT 
TATCCCAAGG AAAGGCCTGA AAAGCTGACA AGTTGCGCCA GCAGCTGTCC CTTAGAGATG 
AAGCTGTGCC CAAGTGTGCA GACGCCTCAG GTACAGAGGG AGAGGGGCCC ACAGGGCCAA 
GGCCATGGCC GCATGGCTCT CAACCTCCAG CTTTCAGACA CTGATGACAA TGAAACGTTT 
GATGAGCTGC ACATAGAGAG CAGTGATGAG AAAAGTCCTT CAGACGTGTC ATTGGCTGCC 
GACACCGATA AGTCCGTGGA GAACCTGGAT GTCCTTGTGG GGTTTGGAAA ATCTCTATGT 
GGGTCTCCTG AAGAGGAGGA AAAACAAGTG CCCATCCCTT CAGAGACTAG GCCAAAGACT 
TTTAGTTTCA TTAAGCAGCA AAGAGTTGTA AAAAGGACTT CTTCAGAAGA ATGTGTGACT 
GTGATATTTG ATGCGGAAGA CGGTGAGCCC ATTGAATTCA GCTCTCACCA GACTGGGGTT 
GTCACTGTTA CCAGAAATGA GATTTCCATC AATTCAACTC CTGCTGGACC CAAGGCAGAA 
CATACTGAGC TTTTACCTCA GGGAATTGCT TGTTTACAGC CAAGAGCTGC TGCAAGAGAC 
TATACTTTCT TTAAAAGGTC TGAAGAGGAC ACTGAGAAAA ACATTCCAAA AGATAATGTA 
GATAATGTTC CCAGGGTGTC CACTGAATCT TTCAGCTCCA GGACAGTGAC ACAAAATCCT 
CAGCAGCAAA AGCTGGTCAA ACCAACACAC AATATATCAT GCCAGAGTAA TTCCAGGTCT 
TCGGCACCCA TGGGCATCTA TCAAAAGCAA AATCTGACAA AAATACCTCC CAGGGGCAAG 
TCTTCACCTC AGAAATCAAA ACTAATGGAG CCCGAAGCCA CCACACTACT CCCTTCATCT 
GGCCTGGTGA CTCTTGAAAA ATCACCAGCC TTAGCTCCTG GGAAACTCTC ACGATTCATG 
AAGACTGAGA GCTCAGGGCC CCTCTTTGAA TTACGATCAG ATCCACACAT TCCAAAACAT 
TCCGCCCAAC TTCCGCACAG CTCCAGGATG CCCAGCAGGA GGGACTGGGT CCAGTGCCCC 
AAGAGTCAGA CTCCAGGGTC ACGGTCAAGG CCTGCCATTG AGTCTAGTGA CAGTGGAGAG 
CCCCCCACGA GGGATGAACA CTGTGGCTCT GGGCCGGAGG CAGGGGTGAA ATCCCCTTCC 
CCTCCGCCCC CTCCAGGCAG GTCCGTCTCC CTGCTGGCCA GGCCCAGCTA TGACTATTCA 
CCAGCACCTT CATCCACCAA GTCCGAAACC AGGGTCCCCA GTGAAACAGC AAGGACCCCA 
TTCAAATCCC CGCTGCTGAA AGGAATTTCT GCTCCAGTTA TTTCTTCTAA TCCGGCCACG 
ACAGAAGTGC AGAGGAAGAA ACCTTCTGTG GCCTTCAAAA AGCCTATCTT CACTCACCCT 
ATGCCCTCCC CAGAAGCAGT CATTCAAACC CGATGCCCTG CTCATGCCCC CTCCAGCTCC 
TTCACCGTAA TGGCTCTGGG GCCTCCAAAG GTCTCTCCGA AGAGAGGTGT CCCCAAAACC 
TCTCCTCGCC AAACACTTGG GACCCCACAA AGGGACATAG GATTACAGAC TCCCAGGATC 
TCTCCTTCAA CCCATGAGCC ACTGGAAATG ACGTCCTCCA AAAGTGTATC TCCAGGGAGA 
AAAGGACAAT TGAATGATAG CGCCTCCACA CCCCCCAAGC CTTCCTTCTT AGGGGTAAAT 
GAGTCACCAT CATCTCAGGT CAGCAGTTCC TCATCATCCT CATCACCCGC CAAAAGCCAT 
AACAGCCCTC ATGGTTGTCA AAGTGCTCAT GAGAAAGGAC TGAAAACTCG CCTTCCAGTG 
GGACTCAAAG TGCTCATGAA GTCTCCCCAG CTGCTCAGGA AAAGTTCCAC CGTGCCAGGG 
AAACATGAAA AAGACAGTTT AAATGAAGCC TCCAAAAGTT CCGTGGCTGT GAACAAGTCT 
AAGCCAGAGG ACTCCAAGAA TCCAGCAAGC ATGGAGATCA CAGCGGGTGA AAGAAATGTG 
ACCCTACCGG ATTCACAAGC ACAGGGCAGT TTAGCTGATG GGCTTCCCCT GGAAACAGCA 
CTACAAGAGC CATTGGAAAG TAGCATCCCT GGGAGTGATG GAAGGGATGG GGTAGATAAT 
AGATCCATGA GAAGATCCCT TTCCTCCAGC AAACCACACC TAAAACCAGC TCTGGGTATG 
AATGGCGCCA AAGCCCGCAG CCACAGCTTC AGTACACACT CAGGAGACAA GCCTTCTACG 
CCCCCCATCG AAGGGTCAGG CAAAGTCCGC ACTCAGATCA TTACCAATAC CGCCGAGAGA 
GGCAATTCTC TTACCCGGCA GAACTCTTCC ACGGAAAGCT CTCCCAACAA GGCCCCTTCT 
GCTCCCATGT TGGAGAGTCT CCCCAGTGTT GGGAGGCCCT CGGGGCACCC CTCCTCCGGG 
AAGGGCTCCC TGGGGAGCTC AGGCAGCTTC AGCAGTCAGC ATGGGAGCCC AAGTAAGTTG 
CCTTTGAGGA TCCCTCCAAA GTCTGAGGGA CTCCTCATCC CACCTGGAAA GGAAGACCAG 
CAGGCCTTCA CCCAGGGAGA GTGCCCCAGT GCCAATGTGG CTGTACTTGG GGAACCAGGC 
AGTGACCGCC GCAGTTGCCC ACCCACCCCA ACAGACTGCC CTGAAGCCCT GCAGAGCCCA 
GGGAGGACTC AGCATCCAAG CACTTTTGAA ACAAGCAGTA CATCCAAGCT AGAAACTTCT 
GGAAGGCATC CAGATGCCTC TGCAACCGCG ACTGATGCTG TGAGTTCAGA AGCCCCCCTC 
TCACCCACAA TCGAAGAAAA GGTCATGTTG TGCATTCAGG AAAATGTGGA AAAGGGCCAA 
GTGCAAACAA AGCCCACCTC TGTGGAAGCA AAGCAGAAGC CTGGGCCTTC TTTTGCCAGC 
TGGTTTGGTT TTCGGAAGAG TAGACTTCCA GCTCTGAGTA GCAGGAAAAT GGACATCTCC 
AAAACCAAAG TAGAAAAGAA AGATGCAAAA GTCTTGGGGT TTGGAAACAG ACAACTAAAA 
TCAGAAAGAA AAAAAGAGAA AAAGAAGCCT GAACTACAGT GTGAGACAGA AAATGAGCTT 
ATCAAGGACA CCAAGTCAGC AGATAATCCA GATGGCGGTT TACAAAGCAA AAATAATCGG 
AGAACACCAC AAGACATTTA CAACCAACTG AAGATTGAAC CAAGGAATAG ACACAGCCCT 
GTTGCATGTT CAACGAAAGA CACCTTCATG ACGGAACTCT TGAACAGAGT TGATAAGAAA 
GCAGCTCCAC AGACAGAAAG TGGATCAAGT AATGCTTCCT GCAGGAATGT GTTAAAGGGC 
AGTTCTCAGG GCTCCTGTCT CATCGGCAGC TCTATCAGTA CTCAAGGAAA CCACAAGAAA 
AACATGAAAA TCAAAGCCGA TATGGAAGTA CCAAAAGACT CCCTGGTAAA AGAGGCAAAT 
GAAAACTTGC AAGAGGATGA AGACGATGCA GTTGCAGATT CTGTATTTCA GAGCCACATC 
ATAGAATCCA ACTGCCAGAT GAGAACATTG GACAGTGGGA TCGGAACCTT TCCACTCCCA 
GACTCGGGAA ATCGCTCGAC AGGACGCTAC CTATGCCAGC CAGACTCCCC AGAGGACGCT 
GAGCCTCTCC TGCCTCTCCA GTCAGCCCTT TCTGCAGTTT CTTCCATGAG AGCCCAAACC 
CTTGAACGTG AAGTGCCTTC CTCCACAGAC GGCCAGCGCC CTGCAGATAG CGCCATTGTT 
CATTCCACAT CCGACCCCAT CATGACCGCC AGAGGGATGA GGCCTCTTCA GAGCCGCCTC 
CCCAAACCAG CTTCCTCAGG AAAAGTCAGT TCCCAAAAGC AGAATGAAGC AGAGCCAAGG 
CCTCAGACAT GCTCATCATT CGGATATGCT GAAGACCCAA TGGCAAGCCA GCCGCTTCCA 
GACTGGGGGA GTGAAGTTGC TGCCACCGGG ACCCAGAAAT TGAGACAACT TGAAGAAACA 
AAAGACGATC CCGAGAATAG ATTATCGAAA ATTTCCCTAG AGTCATTCAA TAAATTTAAC 
AGCAATACTG TGATTTTATT AGAAAAAGAG AAGAACTCTC TGAACAAGGT TGAAGGACAG 
AAGGAAGAAA AAGAAAAAAA TGAAGAGACA TCTTTGAGTA GTTCAGATAG GCCTGGGGTA 
GACAACTTGG AATCTTTGAG TGATTCTTTA TATGATAGCT TCTCTTCCTG TGCCAGTCAA 
GGTTCAAATG ATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_011511103.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509405.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X12, mRNA.

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CloneID	OHu36200
Clone ID Related Accession (Same CDS sequence)	XM_011511104.1 , XM_011511105.2 , XM_011511103.2
Accession Version	XM_011511103.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511103.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGAGA AGCTGATACA TGAACTGGAA GAGGAGAGAC ACTTACGTCT TCAAAGCGAG 
AAGCGGTTGC AGGAGGTGAC CCTAGAGTCT GAACGCAACA GAATTCAGAT GCGTAGCTTG 
CAGCAGCAGT TCTCCAGGAT GGAAGAAACA GTACGAAATC TATTGCAGAG TCAAGGATCT 
CCAGAGCAGA AAAAAGAAGA AACTGTTAAT ATAATGGTCT ATCAGGAAAA GCTGTCAGAG 
GAAGAGAGAA AACATAAGGA AGCTTTGGAA GATCTTCACA TGGTGGTTGA TGAGGATTCG 
AGGAGTGAAA GCAGCAGTAC AGATGAGGGA AAAGAAAAGA CCAAATTGCT ATTAGAGAGA 
TTGAAAGCTC TAGAGGCAGA GAATTCAGCG TTGGCTTTGG AGAATGAAAA TCAAAGGGAA 
CAATATGAGC GATGTCTTGA TGAGGTAGCC AACCAAGTTG TTCAAGCTCT GCTTACTCAA 
AAGGATCTAA GAGAGGAATG TGTGAAGTTG AAAACAAGAG TGTTTGATTT GGAACAGCAG 
AATCGAACAC TAAGCATCCT ATTCCAACAG CGAGTCAGAC CCACTTCTGA TCTGCTCCTC 
CAGAAACTTC ACTCACGTCT CTTGGATCTT TCATCTGGAG ATTTGCTTTC AGAGGTGGAA 
AGAAACCGAA GTCTGACACA GTCACGAACT GATGCTGAGG TGCACGAACA CCAACTAAAT 
ACAAAATCGG CCTTGAAATG CCCTGGCTTG GGGGCTGTCA TCCCTGGTCA TCTCTGTCCT 
CGAAACAGCT ACAGTAGCAG CAGTGAACTG TCTCTTTCAA GCACCTGCAG CGAGTACTCC 
AGTGGCTCCT CCTACACGTG GCACGATGGG AAGAACCTCA GGAAAAGGCA ATCATCACAA 
AACTGGGATA AAAGGCTAAG TATTGATTCT TCGCTCCCAA GTGGCTTTGC TAGTCCTACA 
AATGAACTAC CTCCAACTCG TATCAAGGAA AGCCACATTT TGGAAGGGCT AAGAAAGCTA 
CAGAAGCGAA AAGTGTTACT TGAACCCCCA TCAGTGATAA CCAAATGGGG TTATAAAGAT 
TGCATGAACT CGAATGAAGG AATATATTCT CCTGGAATTA AAAGTAGCAG CCTCAAGGAG 
TATCCCCCCT GCAAAACAGC TGACCTGGGG AGCCCCTGCA AGGAACCCCA CAAGACATTT 
GTTTATGATC TAGATTCCCA CGTTGATGCG GACGATGACC CTTCCACCTT AGCATTGCTC 
CAAGCAGTTC CAAACCAGAG CTGCAGGCCA CATGGCAGTA AATTAACCCA CAGTGTTTCC 
GACAGTCTGT TTGGCTGGGA GACAAACAGA AAACACTTTC TGGAAGGCAC ATCCTCAGTT 
TATCCCAAGG AAAGGCCTGA AAAGCTGACA AGTTGCGCCA GCAGCTGTCC CTTAGAGATG 
AAGCTGTGCC CAAGTGTGCA GACGCCTCAG GTACAGAGGG AGAGGGGCCC ACAGGGCCAA 
GGCCATGGCC GCATGGCTCT CAACCTCCAG CTTTCAGACA CTGATGACAA TGAAACGTTT 
GATGAGCTGC ACATAGAGAG CAGTGATGAG AAAAGTCCTT CAGACGTGTC ATTGGCTGCC 
GACACCGATA AGTCCGTGGA GAACCTGGAT GTCCTTGTGG GGTTTGGAAA ATCTCTATGT 
GGGTCTCCTG AAGAGGAGGA AAAACAAGTG CCCATCCCTT CAGAGACTAG GCCAAAGACT 
TTTAGTTTCA TTAAGCAGCA AAGAGTTGTA AAAAGGACTT CTTCAGAAGA ATGTGTGACT 
GTGATATTTG ATGCGGAAGA CGGTGAGCCC ATTGAATTCA GCTCTCACCA GACTGGGGTT 
GTCACTGTTA CCAGAAATGA GATTTCCATC AATTCAACTC CTGCTGGACC CAAGGCAGAA 
CATACTGAGC TTTTACCTCA GGGAATTGCT TGTTTACAGC CAAGAGCTGC TGCAAGAGAC 
TATACTTTCT TTAAAAGGTC TGAAGAGGAC ACTGAGAAAA ACATTCCAAA AGATAATGTA 
GATAATGTTC CCAGGGTGTC CACTGAATCT TTCAGCTCCA GGACAGTGAC ACAAAATCCT 
CAGCAGCAAA AGCTGGTCAA ACCAACACAC AATATATCAT GCCAGAGTAA TTCCAGGTCT 
TCGGCACCCA TGGGCATCTA TCAAAAGCAA AATCTGACAA AAATACCTCC CAGGGGCAAG 
TCTTCACCTC AGAAATCAAA ACTAATGGAG CCCGAAGCCA CCACACTACT CCCTTCATCT 
GGCCTGGTGA CTCTTGAAAA ATCACCAGCC TTAGCTCCTG GGAAACTCTC ACGATTCATG 
AAGACTGAGA GCTCAGGGCC CCTCTTTGAA TTACGATCAG ATCCACACAT TCCAAAACAT 
TCCGCCCAAC TTCCGCACAG CTCCAGGATG CCCAGCAGGA GGGACTGGGT CCAGTGCCCC 
AAGAGTCAGA CTCCAGGGTC ACGGTCAAGG CCTGCCATTG AGTCTAGTGA CAGTGGAGAG 
CCCCCCACGA GGGATGAACA CTGTGGCTCT GGGCCGGAGG CAGGGGTGAA ATCCCCTTCC 
CCTCCGCCCC CTCCAGGCAG GTCCGTCTCC CTGCTGGCCA GGCCCAGCTA TGACTATTCA 
CCAGCACCTT CATCCACCAA GTCCGAAACC AGGGTCCCCA GTGAAACAGC AAGGACCCCA 
TTCAAATCCC CGCTGCTGAA AGGAATTTCT GCTCCAGTTA TTTCTTCTAA TCCGGCCACG 
ACAGAAGTGC AGAGGAAGAA ACCTTCTGTG GCCTTCAAAA AGCCTATCTT CACTCACCCT 
ATGCCCTCCC CAGAAGCAGT CATTCAAACC CGATGCCCTG CTCATGCCCC CTCCAGCTCC 
TTCACCGTAA TGGCTCTGGG GCCTCCAAAG GTCTCTCCGA AGAGAGGTGT CCCCAAAACC 
TCTCCTCGCC AAACACTTGG GACCCCACAA AGGGACATAG GATTACAGAC TCCCAGGATC 
TCTCCTTCAA CCCATGAGCC ACTGGAAATG ACGTCCTCCA AAAGTGTATC TCCAGGGAGA 
AAAGGACAAT TGAATGATAG CGCCTCCACA CCCCCCAAGC CTTCCTTCTT AGGGGTAAAT 
GAGTCACCAT CATCTCAGGT CAGCAGTTCC TCATCATCCT CATCACCCGC CAAAAGCCAT 
AACAGCCCTC ATGGTTGTCA AAGTGCTCAT GAGAAAGGAC TGAAAACTCG CCTTCCAGTG 
GGACTCAAAG TGCTCATGAA GTCTCCCCAG CTGCTCAGGA AAAGTTCCAC CGTGCCAGGG 
AAACATGAAA AAGACAGTTT AAATGAAGCC TCCAAAAGTT CCGTGGCTGT GAACAAGTCT 
AAGCCAGAGG ACTCCAAGAA TCCAGCAAGC ATGGAGATCA CAGCGGGTGA AAGAAATGTG 
ACCCTACCGG ATTCACAAGC ACAGGGCAGT TTAGCTGATG GGCTTCCCCT GGAAACAGCA 
CTACAAGAGC CATTGGAAAG TAGCATCCCT GGGAGTGATG GAAGGGATGG GGTAGATAAT 
AGATCCATGA GAAGATCCCT TTCCTCCAGC AAACCACACC TAAAACCAGC TCTGGGTATG 
AATGGCGCCA AAGCCCGCAG CCACAGCTTC AGTACACACT CAGGAGACAA GCCTTCTACG 
CCCCCCATCG AAGGGTCAGG CAAAGTCCGC ACTCAGATCA TTACCAATAC CGCCGAGAGA 
GGCAATTCTC TTACCCGGCA GAACTCTTCC ACGGAAAGCT CTCCCAACAA GGCCCCTTCT 
GCTCCCATGT TGGAGAGTCT CCCCAGTGTT GGGAGGCCCT CGGGGCACCC CTCCTCCGGG 
AAGGGCTCCC TGGGGAGCTC AGGCAGCTTC AGCAGTCAGC ATGGGAGCCC AAGTAAGTTG 
CCTTTGAGGA TCCCTCCAAA GTCTGAGGGA CTCCTCATCC CACCTGGAAA GGAAGACCAG 
CAGGCCTTCA CCCAGGGAGA GTGCCCCAGT GCCAATGTGG CTGTACTTGG GGAACCAGGC 
AGTGACCGCC GCAGTTGCCC ACCCACCCCA ACAGACTGCC CTGAAGCCCT GCAGAGCCCA 
GGGAGGACTC AGCATCCAAG CACTTTTGAA ACAAGCAGTA CATCCAAGCT AGAAACTTCT 
GGAAGGCATC CAGATGCCTC TGCAACCGCG ACTGATGCTG TGAGTTCAGA AGCCCCCCTC 
TCACCCACAA TCGAAGAAAA GGTCATGTTG TGCATTCAGG AAAATGTGGA AAAGGGCCAA 
GTGCAAACAA AGCCCACCTC TGTGGAAGCA AAGCAGAAGC CTGGGCCTTC TTTTGCCAGC 
TGGTTTGGTT TTCGGAAGAG TAGACTTCCA GCTCTGAGTA GCAGGAAAAT GGACATCTCC 
AAAACCAAAG TAGAAAAGAA AGATGCAAAA GTCTTGGGGT TTGGAAACAG ACAACTAAAA 
TCAGAAAGAA AAAAAGAGAA AAAGAAGCCT GAACTACAGT GTGAGACAGA AAATGAGCTT 
ATCAAGGACA CCAAGTCAGC AGATAATCCA GATGGCGGTT TACAAAGCAA AAATAATCGG 
AGAACACCAC AAGACATTTA CAACCAACTG AAGATTGAAC CAAGGAATAG ACACAGCCCT 
GTTGCATGTT CAACGAAAGA CACCTTCATG ACGGAACTCT TGAACAGAGT TGATAAGAAA 
GCAGCTCCAC AGACAGAAAG TGGATCAAGT AATGCTTCCT GCAGGAATGT GTTAAAGGGC 
AGTTCTCAGG GCTCCTGTCT CATCGGCAGC TCTATCAGTA CTCAAGGAAA CCACAAGAAA 
AACATGAAAA TCAAAGCCGA TATGGAAGTA CCAAAAGACT CCCTGGTAAA AGAGGCAAAT 
GAAAACTTGC AAGAGGATGA AGACGATGCA GTTGCAGATT CTGTATTTCA GAGCCACATC 
ATAGAATCCA ACTGCCAGAT GAGAACATTG GACAGTGGGA TCGGAACCTT TCCACTCCCA 
GACTCGGGAA ATCGCTCGAC AGGACGCTAC CTATGCCAGC CAGACTCCCC AGAGGACGCT 
GAGCCTCTCC TGCCTCTCCA GTCAGCCCTT TCTGCAGTTT CTTCCATGAG AGCCCAAACC 
CTTGAACGTG AAGTGCCTTC CTCCACAGAC GGCCAGCGCC CTGCAGATAG CGCCATTGTT 
CATTCCACAT CCGACCCCAT CATGACCGCC AGAGGGATGA GGCCTCTTCA GAGCCGCCTC 
CCCAAACCAG CTTCCTCAGG AAAAGTCAGT TCCCAAAAGC AGAATGAAGC AGAGCCAAGG 
CCTCAGACAT GCTCATCATT CGGATATGCT GAAGACCCAA TGGCAAGCCA GCCGCTTCCA 
GACTGGGGGA GTGAAGTTGC TGCCACCGGG ACCCAGAAAT TGAGACAACT TGAAGAAACA 
AAAGACGATC CCGAGAATAG ATTATCGAAA ATTTCCCTAG AGTCATTCAA TAAATTTAAC 
AGCAATACTG TGATTTTATT AGAAAAAGAG AAGAACTCTC TGAACAAGGT TGAAGGACAG 
AAGGAAGAAA AAGAAAAAAA TGAAGAGACA TCTTTGAGTA GTTCAGATAG GCCTGGGGTA 
GACAACTTGG AATCTTTGAG TGATTCTTTA TATGATAGCT TCTCTTCCTG TGCCAGTCAA 
GGTTCAAATG ATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509405.1
CDS	480..6137
Translation	MHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_011511103.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511103.2

ORF Insert Sequence:

ATGCATGAGA AGCTGATACA TGAACTGGAA GAGGAGAGAC ACTTACGTCT TCAAAGCGAG 
AAGCGGTTGC AGGAGGTGAC CCTAGAGTCT GAACGCAACA GAATTCAGAT GCGTAGCTTG 
CAGCAGCAGT TCTCCAGGAT GGAAGAAACA GTACGAAATC TATTGCAGAG TCAAGGATCT 
CCAGAGCAGA AAAAAGAAGA AACTGTTAAT ATAATGGTCT ATCAGGAAAA GCTGTCAGAG 
GAAGAGAGAA AACATAAGGA AGCTTTGGAA GATCTTCACA TGGTGGTTGA TGAGGATTCG 
AGGAGTGAAA GCAGCAGTAC AGATGAGGGA AAAGAAAAGA CCAAATTGCT ATTAGAGAGA 
TTGAAAGCTC TAGAGGCAGA GAATTCAGCG TTGGCTTTGG AGAATGAAAA TCAAAGGGAA 
CAATATGAGC GATGTCTTGA TGAGGTAGCC AACCAAGTTG TTCAAGCTCT GCTTACTCAA 
AAGGATCTAA GAGAGGAATG TGTGAAGTTG AAAACAAGAG TGTTTGATTT GGAACAGCAG 
AATCGAACAC TAAGCATCCT ATTCCAACAG CGAGTCAGAC CCACTTCTGA TCTGCTCCTC 
CAGAAACTTC ACTCACGTCT CTTGGATCTT TCATCTGGAG ATTTGCTTTC AGAGGTGGAA 
AGAAACCGAA GTCTGACACA GTCACGAACT GATGCTGAGG TGCACGAACA CCAACTAAAT 
ACAAAATCGG CCTTGAAATG CCCTGGCTTG GGGGCTGTCA TCCCTGGTCA TCTCTGTCCT 
CGAAACAGCT ACAGTAGCAG CAGTGAACTG TCTCTTTCAA GCACCTGCAG CGAGTACTCC 
AGTGGCTCCT CCTACACGTG GCACGATGGG AAGAACCTCA GGAAAAGGCA ATCATCACAA 
AACTGGGATA AAAGGCTAAG TATTGATTCT TCGCTCCCAA GTGGCTTTGC TAGTCCTACA 
AATGAACTAC CTCCAACTCG TATCAAGGAA AGCCACATTT TGGAAGGGCT AAGAAAGCTA 
CAGAAGCGAA AAGTGTTACT TGAACCCCCA TCAGTGATAA CCAAATGGGG TTATAAAGAT 
TGCATGAACT CGAATGAAGG AATATATTCT CCTGGAATTA AAAGTAGCAG CCTCAAGGAG 
TATCCCCCCT GCAAAACAGC TGACCTGGGG AGCCCCTGCA AGGAACCCCA CAAGACATTT 
GTTTATGATC TAGATTCCCA CGTTGATGCG GACGATGACC CTTCCACCTT AGCATTGCTC 
CAAGCAGTTC CAAACCAGAG CTGCAGGCCA CATGGCAGTA AATTAACCCA CAGTGTTTCC 
GACAGTCTGT TTGGCTGGGA GACAAACAGA AAACACTTTC TGGAAGGCAC ATCCTCAGTT 
TATCCCAAGG AAAGGCCTGA AAAGCTGACA AGTTGCGCCA GCAGCTGTCC CTTAGAGATG 
AAGCTGTGCC CAAGTGTGCA GACGCCTCAG GTACAGAGGG AGAGGGGCCC ACAGGGCCAA 
GGCCATGGCC GCATGGCTCT CAACCTCCAG CTTTCAGACA CTGATGACAA TGAAACGTTT 
GATGAGCTGC ACATAGAGAG CAGTGATGAG AAAAGTCCTT CAGACGTGTC ATTGGCTGCC 
GACACCGATA AGTCCGTGGA GAACCTGGAT GTCCTTGTGG GGTTTGGAAA ATCTCTATGT 
GGGTCTCCTG AAGAGGAGGA AAAACAAGTG CCCATCCCTT CAGAGACTAG GCCAAAGACT 
TTTAGTTTCA TTAAGCAGCA AAGAGTTGTA AAAAGGACTT CTTCAGAAGA ATGTGTGACT 
GTGATATTTG ATGCGGAAGA CGGTGAGCCC ATTGAATTCA GCTCTCACCA GACTGGGGTT 
GTCACTGTTA CCAGAAATGA GATTTCCATC AATTCAACTC CTGCTGGACC CAAGGCAGAA 
CATACTGAGC TTTTACCTCA GGGAATTGCT TGTTTACAGC CAAGAGCTGC TGCAAGAGAC 
TATACTTTCT TTAAAAGGTC TGAAGAGGAC ACTGAGAAAA ACATTCCAAA AGATAATGTA 
GATAATGTTC CCAGGGTGTC CACTGAATCT TTCAGCTCCA GGACAGTGAC ACAAAATCCT 
CAGCAGCAAA AGCTGGTCAA ACCAACACAC AATATATCAT GCCAGAGTAA TTCCAGGTCT 
TCGGCACCCA TGGGCATCTA TCAAAAGCAA AATCTGACAA AAATACCTCC CAGGGGCAAG 
TCTTCACCTC AGAAATCAAA ACTAATGGAG CCCGAAGCCA CCACACTACT CCCTTCATCT 
GGCCTGGTGA CTCTTGAAAA ATCACCAGCC TTAGCTCCTG GGAAACTCTC ACGATTCATG 
AAGACTGAGA GCTCAGGGCC CCTCTTTGAA TTACGATCAG ATCCACACAT TCCAAAACAT 
TCCGCCCAAC TTCCGCACAG CTCCAGGATG CCCAGCAGGA GGGACTGGGT CCAGTGCCCC 
AAGAGTCAGA CTCCAGGGTC ACGGTCAAGG CCTGCCATTG AGTCTAGTGA CAGTGGAGAG 
CCCCCCACGA GGGATGAACA CTGTGGCTCT GGGCCGGAGG CAGGGGTGAA ATCCCCTTCC 
CCTCCGCCCC CTCCAGGCAG GTCCGTCTCC CTGCTGGCCA GGCCCAGCTA TGACTATTCA 
CCAGCACCTT CATCCACCAA GTCCGAAACC AGGGTCCCCA GTGAAACAGC AAGGACCCCA 
TTCAAATCCC CGCTGCTGAA AGGAATTTCT GCTCCAGTTA TTTCTTCTAA TCCGGCCACG 
ACAGAAGTGC AGAGGAAGAA ACCTTCTGTG GCCTTCAAAA AGCCTATCTT CACTCACCCT 
ATGCCCTCCC CAGAAGCAGT CATTCAAACC CGATGCCCTG CTCATGCCCC CTCCAGCTCC 
TTCACCGTAA TGGCTCTGGG GCCTCCAAAG GTCTCTCCGA AGAGAGGTGT CCCCAAAACC 
TCTCCTCGCC AAACACTTGG GACCCCACAA AGGGACATAG GATTACAGAC TCCCAGGATC 
TCTCCTTCAA CCCATGAGCC ACTGGAAATG ACGTCCTCCA AAAGTGTATC TCCAGGGAGA 
AAAGGACAAT TGAATGATAG CGCCTCCACA CCCCCCAAGC CTTCCTTCTT AGGGGTAAAT 
GAGTCACCAT CATCTCAGGT CAGCAGTTCC TCATCATCCT CATCACCCGC CAAAAGCCAT 
AACAGCCCTC ATGGTTGTCA AAGTGCTCAT GAGAAAGGAC TGAAAACTCG CCTTCCAGTG 
GGACTCAAAG TGCTCATGAA GTCTCCCCAG CTGCTCAGGA AAAGTTCCAC CGTGCCAGGG 
AAACATGAAA AAGACAGTTT AAATGAAGCC TCCAAAAGTT CCGTGGCTGT GAACAAGTCT 
AAGCCAGAGG ACTCCAAGAA TCCAGCAAGC ATGGAGATCA CAGCGGGTGA AAGAAATGTG 
ACCCTACCGG ATTCACAAGC ACAGGGCAGT TTAGCTGATG GGCTTCCCCT GGAAACAGCA 
CTACAAGAGC CATTGGAAAG TAGCATCCCT GGGAGTGATG GAAGGGATGG GGTAGATAAT 
AGATCCATGA GAAGATCCCT TTCCTCCAGC AAACCACACC TAAAACCAGC TCTGGGTATG 
AATGGCGCCA AAGCCCGCAG CCACAGCTTC AGTACACACT CAGGAGACAA GCCTTCTACG 
CCCCCCATCG AAGGGTCAGG CAAAGTCCGC ACTCAGATCA TTACCAATAC CGCCGAGAGA 
GGCAATTCTC TTACCCGGCA GAACTCTTCC ACGGAAAGCT CTCCCAACAA GGCCCCTTCT 
GCTCCCATGT TGGAGAGTCT CCCCAGTGTT GGGAGGCCCT CGGGGCACCC CTCCTCCGGG 
AAGGGCTCCC TGGGGAGCTC AGGCAGCTTC AGCAGTCAGC ATGGGAGCCC AAGTAAGTTG 
CCTTTGAGGA TCCCTCCAAA GTCTGAGGGA CTCCTCATCC CACCTGGAAA GGAAGACCAG 
CAGGCCTTCA CCCAGGGAGA GTGCCCCAGT GCCAATGTGG CTGTACTTGG GGAACCAGGC 
AGTGACCGCC GCAGTTGCCC ACCCACCCCA ACAGACTGCC CTGAAGCCCT GCAGAGCCCA 
GGGAGGACTC AGCATCCAAG CACTTTTGAA ACAAGCAGTA CATCCAAGCT AGAAACTTCT 
GGAAGGCATC CAGATGCCTC TGCAACCGCG ACTGATGCTG TGAGTTCAGA AGCCCCCCTC 
TCACCCACAA TCGAAGAAAA GGTCATGTTG TGCATTCAGG AAAATGTGGA AAAGGGCCAA 
GTGCAAACAA AGCCCACCTC TGTGGAAGCA AAGCAGAAGC CTGGGCCTTC TTTTGCCAGC 
TGGTTTGGTT TTCGGAAGAG TAGACTTCCA GCTCTGAGTA GCAGGAAAAT GGACATCTCC 
AAAACCAAAG TAGAAAAGAA AGATGCAAAA GTCTTGGGGT TTGGAAACAG ACAACTAAAA 
TCAGAAAGAA AAAAAGAGAA AAAGAAGCCT GAACTACAGT GTGAGACAGA AAATGAGCTT 
ATCAAGGACA CCAAGTCAGC AGATAATCCA GATGGCGGTT TACAAAGCAA AAATAATCGG 
AGAACACCAC AAGACATTTA CAACCAACTG AAGATTGAAC CAAGGAATAG ACACAGCCCT 
GTTGCATGTT CAACGAAAGA CACCTTCATG ACGGAACTCT TGAACAGAGT TGATAAGAAA 
GCAGCTCCAC AGACAGAAAG TGGATCAAGT AATGCTTCCT GCAGGAATGT GTTAAAGGGC 
AGTTCTCAGG GCTCCTGTCT CATCGGCAGC TCTATCAGTA CTCAAGGAAA CCACAAGAAA 
AACATGAAAA TCAAAGCCGA TATGGAAGTA CCAAAAGACT CCCTGGTAAA AGAGGCAAAT 
GAAAACTTGC AAGAGGATGA AGACGATGCA GTTGCAGATT CTGTATTTCA GAGCCACATC 
ATAGAATCCA ACTGCCAGAT GAGAACATTG GACAGTGGGA TCGGAACCTT TCCACTCCCA 
GACTCGGGAA ATCGCTCGAC AGGACGCTAC CTATGCCAGC CAGACTCCCC AGAGGACGCT 
GAGCCTCTCC TGCCTCTCCA GTCAGCCCTT TCTGCAGTTT CTTCCATGAG AGCCCAAACC 
CTTGAACGTG AAGTGCCTTC CTCCACAGAC GGCCAGCGCC CTGCAGATAG CGCCATTGTT 
CATTCCACAT CCGACCCCAT CATGACCGCC AGAGGGATGA GGCCTCTTCA GAGCCGCCTC 
CCCAAACCAG CTTCCTCAGG AAAAGTCAGT TCCCAAAAGC AGAATGAAGC AGAGCCAAGG 
CCTCAGACAT GCTCATCATT CGGATATGCT GAAGACCCAA TGGCAAGCCA GCCGCTTCCA 
GACTGGGGGA GTGAAGTTGC TGCCACCGGG ACCCAGAAAT TGAGACAACT TGAAGAAACA 
AAAGACGATC CCGAGAATAG ATTATCGAAA ATTTCCCTAG AGTCATTCAA TAAATTTAAC 
AGCAATACTG TGATTTTATT AGAAAAAGAG AAGAACTCTC TGAACAAGGT TGAAGGACAG 
AAGGAAGAAA AAGAAAAAAA TGAAGAGACA TCTTTGAGTA GTTCAGATAG GCCTGGGGTA 
GACAACTTGG AATCTTTGAG TGATTCTTTA TATGATAGCT TCTCTTCCTG TGCCAGTCAA 
GGTTCAAATG ATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_017003976.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859465.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X8, mRNA.

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CloneID	OHu79436
Clone ID Related Accession (Same CDS sequence)	XM_017003976.2 , XM_011511102.2 , XM_017003977.1 , XM_017003977.2 , XM_017003976.1 , XM_011511101.1
Accession Version	XM_017003976.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017003976.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA ATAAATACAT TGAGCATCTG CTGACTCAGC TTGAGGAGCA ACACAGGAGT 
CTCTGGAGGG AGAAGTTGGC AGTGGCCCGA CTACAGCGAG AAGTTGCCCA AAGAACAAGT 
GAGGGGGCCA TGCATGAGAA GCTGATACAT GAACTGGAAG AGGAGAGACA CTTACGTCTT 
CAAAGCGAGA AGCGGTTGCA GGAGGTGACC CTAGAGTCTG AACGCAACAG AATTCAGATG 
CGTAGCTTGC AGCAGCAGTT CTCCAGGATG GAAGAAACAG TACGAAATCT ATTGCAGAGT 
CAAGGATCTC CAGAGCAGAA AAAAGAAGAA ACTGTTAATA TAATGGTCTA TCAGGAAAAG 
CTGTCAGAGG AAGAGAGAAA ACATAAGGAA GCTTTGGAAG ATCTTCACAT GGTGGTTGAT 
GAGGATTCGA GGAGTGAAAG CAGCAGTACA GATGAGGGAA AAGAAAAGAC CAAATTGCTA 
TTAGAGAGAT TGAAAGCTCT AGAGGCAGAG AATTCAGCGT TGGCTTTGGA GAATGAAAAT 
CAAAGGGAAC AATATGAGCG ATGTCTTGAT GAGGTAGCCA ACCAAGTTGT TCAAGCTCTG 
CTTACTCAAA AGGATCTAAG AGAGGAATGT GTGAAGTTGA AAACAAGAGT GTTTGATTTG 
GAACAGCAGA ATCGAACACT AAGCATCCTA TTCCAACAGC GAGTCAGACC CACTTCTGAT 
CTGCTCCTCC AGAAACTTCA CTCACGTCTC TTGGATCTTT CATCTGGAGA TTTGCTTTCA 
GAGGTGGAAA GAAACCGAAG TCTGACACAG TCACGAACTG ATGCTGAGGT GCACGAACAC 
CAACTAAATA CAAAATCGGC CTTGAAATGC CCTGGCTTGG GGGCTGTCAT CCCTGGTCAT 
CTCTGTCCTC GAAACAGCTA CAGTAGCAGC AGTGAACTGT CTCTTTCAAG CACCTGCAGC 
GAGTACTCCA GTGGCTCCTC CTACACGTGG CACGATGGGA AGAACCTCAG GAAAAGGCAA 
TCATCACAAA ACTGGGATAA AAGGCTAAGT ATTGATTCTT CGCTCCCAAG TGGCTTTGCT 
AGTCCTACAA ATGAACTACC TCCAACTCGT ATCAAGGAAA GCCACATTTT GGAAGGGCTA 
AGAAAGCTAC AGAAGCGAAA AGTGTTACTT GAACCCCCAT CAGTGATAAC CAAATGGGGT 
TATAAAGATT GCATGAACTC GAATGAAGGA ATATATTCTC CTGGAATTAA AAGTAGCAGC 
CTCAAGGAGT ATCCCCCCTG CAAAACAGCT GACCTGGGGA GCCCCTGCAA GGAACCCCAC 
AAGACATTTG TTTATGATCT AGATTCCCAC GTTGATGCGG ACGATGACCC TTCCACCTTA 
GCATTGCTCC AAGCAGTTCC AAACCAGAGC TGCAGGCCAC ATGGCAGTAA ATTAACCCAC 
AGTGTTTCCG ACAGTCTGTT TGGCTGGGAG ACAAACAGAA AACACTTTCT GGAAGGCACA 
TCCTCAGTTT ATCCCAAGGA AAGGCCTGAA AAGCTGACAA GTTGCGCCAG CAGCTGTCCC 
TTAGAGATGA AGCTGTGCCC AAGTGTGCAG ACGCCTCAGG TACAGAGGGA GAGGGGCCCA 
CAGGGCCAAG GCCATGGCCG CATGGCTCTC AACCTCCAGC TTTCAGACAC TGATGACAAT 
GAAACGTTTG ATGAGCTGCA CATAGAGAGC AGTGATGAGA AAAGTCCTTC AGACGTGTCA 
TTGGCTGCCG ACACCGATAA GTCCGTGGAG AACCTGGATG TCCTTGTGGG GTTTGGAAAA 
TCTCTATGTG GGTCTCCTGA AGAGGAGGAA AAACAAGTGC CCATCCCTTC AGAGACTAGG 
CCAAAGACTT TTAGTTTCAT TAAGCAGCAA AGAGTTGTAA AAAGGACTTC TTCAGAAGAA 
TGTGTGACTG TGATATTTGA TGCGGAAGAC GGTGAGCCCA TTGAATTCAG CTCTCACCAG 
ACTGGGGTTG TCACTGTTAC CAGAAATGAG ATTTCCATCA ATTCAACTCC TGCTGGACCC 
AAGGCAGAAC ATACTGAGCT TTTACCTCAG GGAATTGCTT GTTTACAGCC AAGAGCTGCT 
GCAAGAGACT ATACTTTCTT TAAAAGGTCT GAAGAGGACA CTGAGAAAAA CATTCCAAAA 
GATAATGTAG ATAATGTTCC CAGGGTGTCC ACTGAATCTT TCAGCTCCAG GACAGTGACA 
CAAAATCCTC AGCAGCAAAA GCTGGTCAAA CCAACACACA ATATATCATG CCAGAGTAAT 
TCCAGGTCTT CGGCACCCAT GGGCATCTAT CAAAAGCAAA ATCTGACAAA AATACCTCCC 
AGGGGCAAGT CTTCACCTCA GAAATCAAAA CTAATGGAGC CCGAAGCCAC CACACTACTC 
CCTTCATCTG GCCTGGTGAC TCTTGAAAAA TCACCAGCCT TAGCTCCTGG GAAACTCTCA 
CGATTCATGA AGACTGAGAG CTCAGGGCCC CTCTTTGAAT TACGATCAGA TCCACACATT 
CCAAAACATT CCGCCCAACT TCCGCACAGC TCCAGGATGC CCAGCAGGAG GGACTGGGTC 
CAGTGCCCCA AGAGTCAGAC TCCAGGGTCA CGGTCAAGGC CTGCCATTGA GTCTAGTGAC 
AGTGGAGAGC CCCCCACGAG GGATGAACAC TGTGGCTCTG GGCCGGAGGC AGGGGTGAAA 
TCCCCTTCCC CTCCGCCCCC TCCAGGCAGG TCCGTCTCCC TGCTGGCCAG GCCCAGCTAT 
GACTATTCAC CAGCACCTTC ATCCACCAAG TCCGAAACCA GGGTCCCCAG TGAAACAGCA 
AGGACCCCAT TCAAATCCCC GCTGCTGAAA GGAATTTCTG CTCCAGTTAT TTCTTCTAAT 
CCGGCCACGA CAGAAGTGCA GAGGAAGAAA CCTTCTGTGG CCTTCAAAAA GCCTATCTTC 
ACTCACCCTA TGCCCTCCCC AGAAGCAGTC ATTCAAACCC GATGCCCTGC TCATGCCCCC 
TCCAGCTCCT TCACCGTAAT GGCTCTGGGG CCTCCAAAGG TCTCTCCGAA GAGAGGTGTC 
CCCAAAACCT CTCCTCGCCA AACACTTGGG ACCCCACAAA GGGACATAGG ATTACAGACT 
CCCAGGATCT CTCCTTCAAC CCATGAGCCA CTGGAAATGA CGTCCTCCAA AAGTGTATCT 
CCAGGGAGAA AAGGACAATT GAATGATAGC GCCTCCACAC CCCCCAAGCC TTCCTTCTTA 
GGGGTAAATG AGTCACCATC ATCTCAGGTC AGCAGTTCCT CATCATCCTC ATCACCCGCC 
AAAAGCCATA ACAGCCCTCA TGGTTGTCAA AGTGCTCATG AGAAAGGACT GAAAACTCGC 
CTTCCAGTGG GACTCAAAGT GCTCATGAAG TCTCCCCAGC TGCTCAGGAA AAGTTCCACC 
GTGCCAGGGA AACATGAAAA AGACAGTTTA AATGAAGCCT CCAAAAGTTC CGTGGCTGTG 
AACAAGTCTA AGCCAGAGGA CTCCAAGAAT CCAGCAAGCA TGGAGATCAC AGCGGGTGAA 
AGAAATGTGA CCCTACCGGA TTCACAAGCA CAGGGCAGTT TAGCTGATGG GCTTCCCCTG 
GAAACAGCAC TACAAGAGCC ATTGGAAAGT AGCATCCCTG GGAGTGATGG AAGGGATGGG 
GTAGATAATA GATCCATGAG AAGATCCCTT TCCTCCAGCA AACCACACCT AAAACCAGCT 
CTGGGTATGA ATGGCGCCAA AGCCCGCAGC CACAGCTTCA GTACACACTC AGGAGACAAG 
CCTTCTACGC CCCCCATCGA AGGGTCAGGC AAAGTCCGCA CTCAGATCAT TACCAATACC 
GCCGAGAGAG GCAATTCTCT TACCCGGCAG AACTCTTCCA CGGAAAGCTC TCCCAACAAG 
GCCCCTTCTG CTCCCATGTT GGAGAGTCTC CCCAGTGTTG GGAGGCCCTC GGGGCACCCC 
TCCTCCGGGA AGGGCTCCCT GGGGAGCTCA GGCAGCTTCA GCAGTCAGCA TGGGAGCCCA 
AGTAAGTTGC CTTTGAGGAT CCCTCCAAAG TCTGAGGGAC TCCTCATCCC ACCTGGAAAG 
GAAGACCAGC AGGCCTTCAC CCAGGGAGAG TGCCCCAGTG CCAATGTGGC TGTACTTGGG 
GAACCAGGCA GTGACCGCCG CAGTTGCCCA CCCACCCCAA CAGACTGCCC TGAAGCCCTG 
CAGAGCCCAG GGAGGACTCA GCATCCAAGC ACTTTTGAAA CAAGCAGTAC ATCCAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCCTCT GCAACCGCGA CTGATGCTGT GAGTTCAGAA 
GCCCCCCTCT CACCCACAAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA 
AAGGGCCAAG TGCAAACAAA GCCCACCTCT GTGGAAGCAA AGCAGAAGCC TGGGCCTTCT 
TTTGCCAGCT GGTTTGGTTT TCGGAAGAGT AGACTTCCAG CTCTGAGTAG CAGGAAAATG 
GACATCTCCA AAACCAAAGT AGAAAAGAAA GATGCAAAAG TCTTGGGGTT TGGAAACAGA 
CAACTAAAAT CAGAAAGAAA AAAAGAGAAA AAGAAGCCTG AACTACAGTG TGAGACAGAA 
AATGAGCTTA TCAAGGACAC CAAGTCAGCA GATAATCCAG ATGGCGGTTT ACAAAGCAAA 
AATAATCGGA GAACACCACA AGACATTTAC AACCAACTGA AGATTGAACC AAGGAATAGA 
CACAGCCCTG TTGCATGTTC AACGAAAGAC ACCTTCATGA CGGAACTCTT GAACAGAGTT 
GATAAGAAAG CAGCTCCACA GACAGAAAGT GGATCAAGTA ATGCTTCCTG CAGGAATGTG 
TTAAAGGGCA GTTCTCAGGG CTCCTGTCTC ATCGGCAGCT CTATCAGTAC TCAAGGAAAC 
CACAAGAAAA ACATGAAAAT CAAAGCCGAT ATGGAAGTAC CAAAAGACTC CCTGGTAAAA 
GAGGCAAATG AAAACTTGCA AGAGGATGAA GACGATGCAG TTGCAGATTC TGTATTTCAG 
AGCCACATCA TAGAATCCAA CTGCCAGATG AGAACATTGG ACAGTGGGAT CGGAACCTTT 
CCACTCCCAG ACTCGGGAAA TCGCTCGACA GGACGCTACC TATGCCAGCC AGACTCCCCA 
GAGGACGCTG AGCCTCTCCT GCCTCTCCAG TCAGCCCTTT CTGCAGTTTC TTCCATGAGA 
GCCCAAACCC TTGAACGTGA AGTGCCTTCC TCCACAGACG GCCAGCGCCC TGCAGATAGC 
GCCATTGTTC ATTCCACATC CGACCCCATC ATGACCGCCA GAGGGATGAG GCCTCTTCAG 
AGCCGCCTCC CCAAACCAGC TTCCTCAGGA AAAGTCAGTT CCCAAAAGCA GAATGAAGCA 
GAGCCAAGGC CTCAGACATG CTCATCATTC GGATATGCTG AAGACCCAAT GGCAAGCCAG 
CCGCTTCCAG ACTGGGGGAG TGAAGTTGCT GCCACCGGGA CCCAGAAATT GAGACAACTT 
GAAGAAACAA AAGACGATCC CGAGAATAGA TTATCGAAAA TTTCCCTAGA GTCATTCAAT 
AAATTTAACA GCAATACTGT GATTTTATTA GAAAAAGAGA AGAACTCTCT GAACAAGGTT 
GAAGGACAGA AGGAAGAAAA AGAAAAAAAT GAAGAGACAT CTTTGAGTAG TTCAGATAGG 
CCTGGGGTAG ACAACTTGGA ATCTTTGAGT GATTCTTTAT ATGATAGCTT CTCTTCCTGT 
GCCAGTCAAG GTTCAAATGA TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859465.1
CDS	1385..7171
Translation	MDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_017003976.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003976.2

ORF Insert Sequence:

ATGGACTCCA ATAAATACAT TGAGCATCTG CTGACTCAGC TTGAGGAGCA ACACAGGAGT 
CTCTGGAGGG AGAAGTTGGC AGTGGCCCGA CTACAGCGAG AAGTTGCCCA AAGAACAAGT 
GAGGGGGCCA TGCATGAGAA GCTGATACAT GAACTGGAAG AGGAGAGACA CTTACGTCTT 
CAAAGCGAGA AGCGGTTGCA GGAGGTGACC CTAGAGTCTG AACGCAACAG AATTCAGATG 
CGTAGCTTGC AGCAGCAGTT CTCCAGGATG GAAGAAACAG TACGAAATCT ATTGCAGAGT 
CAAGGATCTC CAGAGCAGAA AAAAGAAGAA ACTGTTAATA TAATGGTCTA TCAGGAAAAG 
CTGTCAGAGG AAGAGAGAAA ACATAAGGAA GCTTTGGAAG ATCTTCACAT GGTGGTTGAT 
GAGGATTCGA GGAGTGAAAG CAGCAGTACA GATGAGGGAA AAGAAAAGAC CAAATTGCTA 
TTAGAGAGAT TGAAAGCTCT AGAGGCAGAG AATTCAGCGT TGGCTTTGGA GAATGAAAAT 
CAAAGGGAAC AATATGAGCG ATGTCTTGAT GAGGTAGCCA ACCAAGTTGT TCAAGCTCTG 
CTTACTCAAA AGGATCTAAG AGAGGAATGT GTGAAGTTGA AAACAAGAGT GTTTGATTTG 
GAACAGCAGA ATCGAACACT AAGCATCCTA TTCCAACAGC GAGTCAGACC CACTTCTGAT 
CTGCTCCTCC AGAAACTTCA CTCACGTCTC TTGGATCTTT CATCTGGAGA TTTGCTTTCA 
GAGGTGGAAA GAAACCGAAG TCTGACACAG TCACGAACTG ATGCTGAGGT GCACGAACAC 
CAACTAAATA CAAAATCGGC CTTGAAATGC CCTGGCTTGG GGGCTGTCAT CCCTGGTCAT 
CTCTGTCCTC GAAACAGCTA CAGTAGCAGC AGTGAACTGT CTCTTTCAAG CACCTGCAGC 
GAGTACTCCA GTGGCTCCTC CTACACGTGG CACGATGGGA AGAACCTCAG GAAAAGGCAA 
TCATCACAAA ACTGGGATAA AAGGCTAAGT ATTGATTCTT CGCTCCCAAG TGGCTTTGCT 
AGTCCTACAA ATGAACTACC TCCAACTCGT ATCAAGGAAA GCCACATTTT GGAAGGGCTA 
AGAAAGCTAC AGAAGCGAAA AGTGTTACTT GAACCCCCAT CAGTGATAAC CAAATGGGGT 
TATAAAGATT GCATGAACTC GAATGAAGGA ATATATTCTC CTGGAATTAA AAGTAGCAGC 
CTCAAGGAGT ATCCCCCCTG CAAAACAGCT GACCTGGGGA GCCCCTGCAA GGAACCCCAC 
AAGACATTTG TTTATGATCT AGATTCCCAC GTTGATGCGG ACGATGACCC TTCCACCTTA 
GCATTGCTCC AAGCAGTTCC AAACCAGAGC TGCAGGCCAC ATGGCAGTAA ATTAACCCAC 
AGTGTTTCCG ACAGTCTGTT TGGCTGGGAG ACAAACAGAA AACACTTTCT GGAAGGCACA 
TCCTCAGTTT ATCCCAAGGA AAGGCCTGAA AAGCTGACAA GTTGCGCCAG CAGCTGTCCC 
TTAGAGATGA AGCTGTGCCC AAGTGTGCAG ACGCCTCAGG TACAGAGGGA GAGGGGCCCA 
CAGGGCCAAG GCCATGGCCG CATGGCTCTC AACCTCCAGC TTTCAGACAC TGATGACAAT 
GAAACGTTTG ATGAGCTGCA CATAGAGAGC AGTGATGAGA AAAGTCCTTC AGACGTGTCA 
TTGGCTGCCG ACACCGATAA GTCCGTGGAG AACCTGGATG TCCTTGTGGG GTTTGGAAAA 
TCTCTATGTG GGTCTCCTGA AGAGGAGGAA AAACAAGTGC CCATCCCTTC AGAGACTAGG 
CCAAAGACTT TTAGTTTCAT TAAGCAGCAA AGAGTTGTAA AAAGGACTTC TTCAGAAGAA 
TGTGTGACTG TGATATTTGA TGCGGAAGAC GGTGAGCCCA TTGAATTCAG CTCTCACCAG 
ACTGGGGTTG TCACTGTTAC CAGAAATGAG ATTTCCATCA ATTCAACTCC TGCTGGACCC 
AAGGCAGAAC ATACTGAGCT TTTACCTCAG GGAATTGCTT GTTTACAGCC AAGAGCTGCT 
GCAAGAGACT ATACTTTCTT TAAAAGGTCT GAAGAGGACA CTGAGAAAAA CATTCCAAAA 
GATAATGTAG ATAATGTTCC CAGGGTGTCC ACTGAATCTT TCAGCTCCAG GACAGTGACA 
CAAAATCCTC AGCAGCAAAA GCTGGTCAAA CCAACACACA ATATATCATG CCAGAGTAAT 
TCCAGGTCTT CGGCACCCAT GGGCATCTAT CAAAAGCAAA ATCTGACAAA AATACCTCCC 
AGGGGCAAGT CTTCACCTCA GAAATCAAAA CTAATGGAGC CCGAAGCCAC CACACTACTC 
CCTTCATCTG GCCTGGTGAC TCTTGAAAAA TCACCAGCCT TAGCTCCTGG GAAACTCTCA 
CGATTCATGA AGACTGAGAG CTCAGGGCCC CTCTTTGAAT TACGATCAGA TCCACACATT 
CCAAAACATT CCGCCCAACT TCCGCACAGC TCCAGGATGC CCAGCAGGAG GGACTGGGTC 
CAGTGCCCCA AGAGTCAGAC TCCAGGGTCA CGGTCAAGGC CTGCCATTGA GTCTAGTGAC 
AGTGGAGAGC CCCCCACGAG GGATGAACAC TGTGGCTCTG GGCCGGAGGC AGGGGTGAAA 
TCCCCTTCCC CTCCGCCCCC TCCAGGCAGG TCCGTCTCCC TGCTGGCCAG GCCCAGCTAT 
GACTATTCAC CAGCACCTTC ATCCACCAAG TCCGAAACCA GGGTCCCCAG TGAAACAGCA 
AGGACCCCAT TCAAATCCCC GCTGCTGAAA GGAATTTCTG CTCCAGTTAT TTCTTCTAAT 
CCGGCCACGA CAGAAGTGCA GAGGAAGAAA CCTTCTGTGG CCTTCAAAAA GCCTATCTTC 
ACTCACCCTA TGCCCTCCCC AGAAGCAGTC ATTCAAACCC GATGCCCTGC TCATGCCCCC 
TCCAGCTCCT TCACCGTAAT GGCTCTGGGG CCTCCAAAGG TCTCTCCGAA GAGAGGTGTC 
CCCAAAACCT CTCCTCGCCA AACACTTGGG ACCCCACAAA GGGACATAGG ATTACAGACT 
CCCAGGATCT CTCCTTCAAC CCATGAGCCA CTGGAAATGA CGTCCTCCAA AAGTGTATCT 
CCAGGGAGAA AAGGACAATT GAATGATAGC GCCTCCACAC CCCCCAAGCC TTCCTTCTTA 
GGGGTAAATG AGTCACCATC ATCTCAGGTC AGCAGTTCCT CATCATCCTC ATCACCCGCC 
AAAAGCCATA ACAGCCCTCA TGGTTGTCAA AGTGCTCATG AGAAAGGACT GAAAACTCGC 
CTTCCAGTGG GACTCAAAGT GCTCATGAAG TCTCCCCAGC TGCTCAGGAA AAGTTCCACC 
GTGCCAGGGA AACATGAAAA AGACAGTTTA AATGAAGCCT CCAAAAGTTC CGTGGCTGTG 
AACAAGTCTA AGCCAGAGGA CTCCAAGAAT CCAGCAAGCA TGGAGATCAC AGCGGGTGAA 
AGAAATGTGA CCCTACCGGA TTCACAAGCA CAGGGCAGTT TAGCTGATGG GCTTCCCCTG 
GAAACAGCAC TACAAGAGCC ATTGGAAAGT AGCATCCCTG GGAGTGATGG AAGGGATGGG 
GTAGATAATA GATCCATGAG AAGATCCCTT TCCTCCAGCA AACCACACCT AAAACCAGCT 
CTGGGTATGA ATGGCGCCAA AGCCCGCAGC CACAGCTTCA GTACACACTC AGGAGACAAG 
CCTTCTACGC CCCCCATCGA AGGGTCAGGC AAAGTCCGCA CTCAGATCAT TACCAATACC 
GCCGAGAGAG GCAATTCTCT TACCCGGCAG AACTCTTCCA CGGAAAGCTC TCCCAACAAG 
GCCCCTTCTG CTCCCATGTT GGAGAGTCTC CCCAGTGTTG GGAGGCCCTC GGGGCACCCC 
TCCTCCGGGA AGGGCTCCCT GGGGAGCTCA GGCAGCTTCA GCAGTCAGCA TGGGAGCCCA 
AGTAAGTTGC CTTTGAGGAT CCCTCCAAAG TCTGAGGGAC TCCTCATCCC ACCTGGAAAG 
GAAGACCAGC AGGCCTTCAC CCAGGGAGAG TGCCCCAGTG CCAATGTGGC TGTACTTGGG 
GAACCAGGCA GTGACCGCCG CAGTTGCCCA CCCACCCCAA CAGACTGCCC TGAAGCCCTG 
CAGAGCCCAG GGAGGACTCA GCATCCAAGC ACTTTTGAAA CAAGCAGTAC ATCCAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCCTCT GCAACCGCGA CTGATGCTGT GAGTTCAGAA 
GCCCCCCTCT CACCCACAAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA 
AAGGGCCAAG TGCAAACAAA GCCCACCTCT GTGGAAGCAA AGCAGAAGCC TGGGCCTTCT 
TTTGCCAGCT GGTTTGGTTT TCGGAAGAGT AGACTTCCAG CTCTGAGTAG CAGGAAAATG 
GACATCTCCA AAACCAAAGT AGAAAAGAAA GATGCAAAAG TCTTGGGGTT TGGAAACAGA 
CAACTAAAAT CAGAAAGAAA AAAAGAGAAA AAGAAGCCTG AACTACAGTG TGAGACAGAA 
AATGAGCTTA TCAAGGACAC CAAGTCAGCA GATAATCCAG ATGGCGGTTT ACAAAGCAAA 
AATAATCGGA GAACACCACA AGACATTTAC AACCAACTGA AGATTGAACC AAGGAATAGA 
CACAGCCCTG TTGCATGTTC AACGAAAGAC ACCTTCATGA CGGAACTCTT GAACAGAGTT 
GATAAGAAAG CAGCTCCACA GACAGAAAGT GGATCAAGTA ATGCTTCCTG CAGGAATGTG 
TTAAAGGGCA GTTCTCAGGG CTCCTGTCTC ATCGGCAGCT CTATCAGTAC TCAAGGAAAC 
CACAAGAAAA ACATGAAAAT CAAAGCCGAT ATGGAAGTAC CAAAAGACTC CCTGGTAAAA 
GAGGCAAATG AAAACTTGCA AGAGGATGAA GACGATGCAG TTGCAGATTC TGTATTTCAG 
AGCCACATCA TAGAATCCAA CTGCCAGATG AGAACATTGG ACAGTGGGAT CGGAACCTTT 
CCACTCCCAG ACTCGGGAAA TCGCTCGACA GGACGCTACC TATGCCAGCC AGACTCCCCA 
GAGGACGCTG AGCCTCTCCT GCCTCTCCAG TCAGCCCTTT CTGCAGTTTC TTCCATGAGA 
GCCCAAACCC TTGAACGTGA AGTGCCTTCC TCCACAGACG GCCAGCGCCC TGCAGATAGC 
GCCATTGTTC ATTCCACATC CGACCCCATC ATGACCGCCA GAGGGATGAG GCCTCTTCAG 
AGCCGCCTCC CCAAACCAGC TTCCTCAGGA AAAGTCAGTT CCCAAAAGCA GAATGAAGCA 
GAGCCAAGGC CTCAGACATG CTCATCATTC GGATATGCTG AAGACCCAAT GGCAAGCCAG 
CCGCTTCCAG ACTGGGGGAG TGAAGTTGCT GCCACCGGGA CCCAGAAATT GAGACAACTT 
GAAGAAACAA AAGACGATCC CGAGAATAGA TTATCGAAAA TTTCCCTAGA GTCATTCAAT 
AAATTTAACA GCAATACTGT GATTTTATTA GAAAAAGAGA AGAACTCTCT GAACAAGGTT 
GAAGGACAGA AGGAAGAAAA AGAAAAAAAT GAAGAGACAT CTTTGAGTAG TTCAGATAGG 
CCTGGGGTAG ACAACTTGGA ATCTTTGAGT GATTCTTTAT ATGATAGCTT CTCTTCCTGT 
GCCAGTCAAG GTTCAAATGA TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_011511102.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509404.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X10, mRNA.

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CloneID	OHu79436
Clone ID Related Accession (Same CDS sequence)	XM_017003976.2 , XM_011511102.2 , XM_017003977.1 , XM_017003977.2 , XM_017003976.1 , XM_011511101.1
Accession Version	XM_011511102.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511102.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA ATAAATACAT TGAGCATCTG CTGACTCAGC TTGAGGAGCA ACACAGGAGT 
CTCTGGAGGG AGAAGTTGGC AGTGGCCCGA CTACAGCGAG AAGTTGCCCA AAGAACAAGT 
GAGGGGGCCA TGCATGAGAA GCTGATACAT GAACTGGAAG AGGAGAGACA CTTACGTCTT 
CAAAGCGAGA AGCGGTTGCA GGAGGTGACC CTAGAGTCTG AACGCAACAG AATTCAGATG 
CGTAGCTTGC AGCAGCAGTT CTCCAGGATG GAAGAAACAG TACGAAATCT ATTGCAGAGT 
CAAGGATCTC CAGAGCAGAA AAAAGAAGAA ACTGTTAATA TAATGGTCTA TCAGGAAAAG 
CTGTCAGAGG AAGAGAGAAA ACATAAGGAA GCTTTGGAAG ATCTTCACAT GGTGGTTGAT 
GAGGATTCGA GGAGTGAAAG CAGCAGTACA GATGAGGGAA AAGAAAAGAC CAAATTGCTA 
TTAGAGAGAT TGAAAGCTCT AGAGGCAGAG AATTCAGCGT TGGCTTTGGA GAATGAAAAT 
CAAAGGGAAC AATATGAGCG ATGTCTTGAT GAGGTAGCCA ACCAAGTTGT TCAAGCTCTG 
CTTACTCAAA AGGATCTAAG AGAGGAATGT GTGAAGTTGA AAACAAGAGT GTTTGATTTG 
GAACAGCAGA ATCGAACACT AAGCATCCTA TTCCAACAGC GAGTCAGACC CACTTCTGAT 
CTGCTCCTCC AGAAACTTCA CTCACGTCTC TTGGATCTTT CATCTGGAGA TTTGCTTTCA 
GAGGTGGAAA GAAACCGAAG TCTGACACAG TCACGAACTG ATGCTGAGGT GCACGAACAC 
CAACTAAATA CAAAATCGGC CTTGAAATGC CCTGGCTTGG GGGCTGTCAT CCCTGGTCAT 
CTCTGTCCTC GAAACAGCTA CAGTAGCAGC AGTGAACTGT CTCTTTCAAG CACCTGCAGC 
GAGTACTCCA GTGGCTCCTC CTACACGTGG CACGATGGGA AGAACCTCAG GAAAAGGCAA 
TCATCACAAA ACTGGGATAA AAGGCTAAGT ATTGATTCTT CGCTCCCAAG TGGCTTTGCT 
AGTCCTACAA ATGAACTACC TCCAACTCGT ATCAAGGAAA GCCACATTTT GGAAGGGCTA 
AGAAAGCTAC AGAAGCGAAA AGTGTTACTT GAACCCCCAT CAGTGATAAC CAAATGGGGT 
TATAAAGATT GCATGAACTC GAATGAAGGA ATATATTCTC CTGGAATTAA AAGTAGCAGC 
CTCAAGGAGT ATCCCCCCTG CAAAACAGCT GACCTGGGGA GCCCCTGCAA GGAACCCCAC 
AAGACATTTG TTTATGATCT AGATTCCCAC GTTGATGCGG ACGATGACCC TTCCACCTTA 
GCATTGCTCC AAGCAGTTCC AAACCAGAGC TGCAGGCCAC ATGGCAGTAA ATTAACCCAC 
AGTGTTTCCG ACAGTCTGTT TGGCTGGGAG ACAAACAGAA AACACTTTCT GGAAGGCACA 
TCCTCAGTTT ATCCCAAGGA AAGGCCTGAA AAGCTGACAA GTTGCGCCAG CAGCTGTCCC 
TTAGAGATGA AGCTGTGCCC AAGTGTGCAG ACGCCTCAGG TACAGAGGGA GAGGGGCCCA 
CAGGGCCAAG GCCATGGCCG CATGGCTCTC AACCTCCAGC TTTCAGACAC TGATGACAAT 
GAAACGTTTG ATGAGCTGCA CATAGAGAGC AGTGATGAGA AAAGTCCTTC AGACGTGTCA 
TTGGCTGCCG ACACCGATAA GTCCGTGGAG AACCTGGATG TCCTTGTGGG GTTTGGAAAA 
TCTCTATGTG GGTCTCCTGA AGAGGAGGAA AAACAAGTGC CCATCCCTTC AGAGACTAGG 
CCAAAGACTT TTAGTTTCAT TAAGCAGCAA AGAGTTGTAA AAAGGACTTC TTCAGAAGAA 
TGTGTGACTG TGATATTTGA TGCGGAAGAC GGTGAGCCCA TTGAATTCAG CTCTCACCAG 
ACTGGGGTTG TCACTGTTAC CAGAAATGAG ATTTCCATCA ATTCAACTCC TGCTGGACCC 
AAGGCAGAAC ATACTGAGCT TTTACCTCAG GGAATTGCTT GTTTACAGCC AAGAGCTGCT 
GCAAGAGACT ATACTTTCTT TAAAAGGTCT GAAGAGGACA CTGAGAAAAA CATTCCAAAA 
GATAATGTAG ATAATGTTCC CAGGGTGTCC ACTGAATCTT TCAGCTCCAG GACAGTGACA 
CAAAATCCTC AGCAGCAAAA GCTGGTCAAA CCAACACACA ATATATCATG CCAGAGTAAT 
TCCAGGTCTT CGGCACCCAT GGGCATCTAT CAAAAGCAAA ATCTGACAAA AATACCTCCC 
AGGGGCAAGT CTTCACCTCA GAAATCAAAA CTAATGGAGC CCGAAGCCAC CACACTACTC 
CCTTCATCTG GCCTGGTGAC TCTTGAAAAA TCACCAGCCT TAGCTCCTGG GAAACTCTCA 
CGATTCATGA AGACTGAGAG CTCAGGGCCC CTCTTTGAAT TACGATCAGA TCCACACATT 
CCAAAACATT CCGCCCAACT TCCGCACAGC TCCAGGATGC CCAGCAGGAG GGACTGGGTC 
CAGTGCCCCA AGAGTCAGAC TCCAGGGTCA CGGTCAAGGC CTGCCATTGA GTCTAGTGAC 
AGTGGAGAGC CCCCCACGAG GGATGAACAC TGTGGCTCTG GGCCGGAGGC AGGGGTGAAA 
TCCCCTTCCC CTCCGCCCCC TCCAGGCAGG TCCGTCTCCC TGCTGGCCAG GCCCAGCTAT 
GACTATTCAC CAGCACCTTC ATCCACCAAG TCCGAAACCA GGGTCCCCAG TGAAACAGCA 
AGGACCCCAT TCAAATCCCC GCTGCTGAAA GGAATTTCTG CTCCAGTTAT TTCTTCTAAT 
CCGGCCACGA CAGAAGTGCA GAGGAAGAAA CCTTCTGTGG CCTTCAAAAA GCCTATCTTC 
ACTCACCCTA TGCCCTCCCC AGAAGCAGTC ATTCAAACCC GATGCCCTGC TCATGCCCCC 
TCCAGCTCCT TCACCGTAAT GGCTCTGGGG CCTCCAAAGG TCTCTCCGAA GAGAGGTGTC 
CCCAAAACCT CTCCTCGCCA AACACTTGGG ACCCCACAAA GGGACATAGG ATTACAGACT 
CCCAGGATCT CTCCTTCAAC CCATGAGCCA CTGGAAATGA CGTCCTCCAA AAGTGTATCT 
CCAGGGAGAA AAGGACAATT GAATGATAGC GCCTCCACAC CCCCCAAGCC TTCCTTCTTA 
GGGGTAAATG AGTCACCATC ATCTCAGGTC AGCAGTTCCT CATCATCCTC ATCACCCGCC 
AAAAGCCATA ACAGCCCTCA TGGTTGTCAA AGTGCTCATG AGAAAGGACT GAAAACTCGC 
CTTCCAGTGG GACTCAAAGT GCTCATGAAG TCTCCCCAGC TGCTCAGGAA AAGTTCCACC 
GTGCCAGGGA AACATGAAAA AGACAGTTTA AATGAAGCCT CCAAAAGTTC CGTGGCTGTG 
AACAAGTCTA AGCCAGAGGA CTCCAAGAAT CCAGCAAGCA TGGAGATCAC AGCGGGTGAA 
AGAAATGTGA CCCTACCGGA TTCACAAGCA CAGGGCAGTT TAGCTGATGG GCTTCCCCTG 
GAAACAGCAC TACAAGAGCC ATTGGAAAGT AGCATCCCTG GGAGTGATGG AAGGGATGGG 
GTAGATAATA GATCCATGAG AAGATCCCTT TCCTCCAGCA AACCACACCT AAAACCAGCT 
CTGGGTATGA ATGGCGCCAA AGCCCGCAGC CACAGCTTCA GTACACACTC AGGAGACAAG 
CCTTCTACGC CCCCCATCGA AGGGTCAGGC AAAGTCCGCA CTCAGATCAT TACCAATACC 
GCCGAGAGAG GCAATTCTCT TACCCGGCAG AACTCTTCCA CGGAAAGCTC TCCCAACAAG 
GCCCCTTCTG CTCCCATGTT GGAGAGTCTC CCCAGTGTTG GGAGGCCCTC GGGGCACCCC 
TCCTCCGGGA AGGGCTCCCT GGGGAGCTCA GGCAGCTTCA GCAGTCAGCA TGGGAGCCCA 
AGTAAGTTGC CTTTGAGGAT CCCTCCAAAG TCTGAGGGAC TCCTCATCCC ACCTGGAAAG 
GAAGACCAGC AGGCCTTCAC CCAGGGAGAG TGCCCCAGTG CCAATGTGGC TGTACTTGGG 
GAACCAGGCA GTGACCGCCG CAGTTGCCCA CCCACCCCAA CAGACTGCCC TGAAGCCCTG 
CAGAGCCCAG GGAGGACTCA GCATCCAAGC ACTTTTGAAA CAAGCAGTAC ATCCAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCCTCT GCAACCGCGA CTGATGCTGT GAGTTCAGAA 
GCCCCCCTCT CACCCACAAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA 
AAGGGCCAAG TGCAAACAAA GCCCACCTCT GTGGAAGCAA AGCAGAAGCC TGGGCCTTCT 
TTTGCCAGCT GGTTTGGTTT TCGGAAGAGT AGACTTCCAG CTCTGAGTAG CAGGAAAATG 
GACATCTCCA AAACCAAAGT AGAAAAGAAA GATGCAAAAG TCTTGGGGTT TGGAAACAGA 
CAACTAAAAT CAGAAAGAAA AAAAGAGAAA AAGAAGCCTG AACTACAGTG TGAGACAGAA 
AATGAGCTTA TCAAGGACAC CAAGTCAGCA GATAATCCAG ATGGCGGTTT ACAAAGCAAA 
AATAATCGGA GAACACCACA AGACATTTAC AACCAACTGA AGATTGAACC AAGGAATAGA 
CACAGCCCTG TTGCATGTTC AACGAAAGAC ACCTTCATGA CGGAACTCTT GAACAGAGTT 
GATAAGAAAG CAGCTCCACA GACAGAAAGT GGATCAAGTA ATGCTTCCTG CAGGAATGTG 
TTAAAGGGCA GTTCTCAGGG CTCCTGTCTC ATCGGCAGCT CTATCAGTAC TCAAGGAAAC 
CACAAGAAAA ACATGAAAAT CAAAGCCGAT ATGGAAGTAC CAAAAGACTC CCTGGTAAAA 
GAGGCAAATG AAAACTTGCA AGAGGATGAA GACGATGCAG TTGCAGATTC TGTATTTCAG 
AGCCACATCA TAGAATCCAA CTGCCAGATG AGAACATTGG ACAGTGGGAT CGGAACCTTT 
CCACTCCCAG ACTCGGGAAA TCGCTCGACA GGACGCTACC TATGCCAGCC AGACTCCCCA 
GAGGACGCTG AGCCTCTCCT GCCTCTCCAG TCAGCCCTTT CTGCAGTTTC TTCCATGAGA 
GCCCAAACCC TTGAACGTGA AGTGCCTTCC TCCACAGACG GCCAGCGCCC TGCAGATAGC 
GCCATTGTTC ATTCCACATC CGACCCCATC ATGACCGCCA GAGGGATGAG GCCTCTTCAG 
AGCCGCCTCC CCAAACCAGC TTCCTCAGGA AAAGTCAGTT CCCAAAAGCA GAATGAAGCA 
GAGCCAAGGC CTCAGACATG CTCATCATTC GGATATGCTG AAGACCCAAT GGCAAGCCAG 
CCGCTTCCAG ACTGGGGGAG TGAAGTTGCT GCCACCGGGA CCCAGAAATT GAGACAACTT 
GAAGAAACAA AAGACGATCC CGAGAATAGA TTATCGAAAA TTTCCCTAGA GTCATTCAAT 
AAATTTAACA GCAATACTGT GATTTTATTA GAAAAAGAGA AGAACTCTCT GAACAAGGTT 
GAAGGACAGA AGGAAGAAAA AGAAAAAAAT GAAGAGACAT CTTTGAGTAG TTCAGATAGG 
CCTGGGGTAG ACAACTTGGA ATCTTTGAGT GATTCTTTAT ATGATAGCTT CTCTTCCTGT 
GCCAGTCAAG GTTCAAATGA TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509404.1
CDS	4549..10335
Translation	MDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_011511102.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511102.2

ORF Insert Sequence:

ATGGACTCCA ATAAATACAT TGAGCATCTG CTGACTCAGC TTGAGGAGCA ACACAGGAGT 
CTCTGGAGGG AGAAGTTGGC AGTGGCCCGA CTACAGCGAG AAGTTGCCCA AAGAACAAGT 
GAGGGGGCCA TGCATGAGAA GCTGATACAT GAACTGGAAG AGGAGAGACA CTTACGTCTT 
CAAAGCGAGA AGCGGTTGCA GGAGGTGACC CTAGAGTCTG AACGCAACAG AATTCAGATG 
CGTAGCTTGC AGCAGCAGTT CTCCAGGATG GAAGAAACAG TACGAAATCT ATTGCAGAGT 
CAAGGATCTC CAGAGCAGAA AAAAGAAGAA ACTGTTAATA TAATGGTCTA TCAGGAAAAG 
CTGTCAGAGG AAGAGAGAAA ACATAAGGAA GCTTTGGAAG ATCTTCACAT GGTGGTTGAT 
GAGGATTCGA GGAGTGAAAG CAGCAGTACA GATGAGGGAA AAGAAAAGAC CAAATTGCTA 
TTAGAGAGAT TGAAAGCTCT AGAGGCAGAG AATTCAGCGT TGGCTTTGGA GAATGAAAAT 
CAAAGGGAAC AATATGAGCG ATGTCTTGAT GAGGTAGCCA ACCAAGTTGT TCAAGCTCTG 
CTTACTCAAA AGGATCTAAG AGAGGAATGT GTGAAGTTGA AAACAAGAGT GTTTGATTTG 
GAACAGCAGA ATCGAACACT AAGCATCCTA TTCCAACAGC GAGTCAGACC CACTTCTGAT 
CTGCTCCTCC AGAAACTTCA CTCACGTCTC TTGGATCTTT CATCTGGAGA TTTGCTTTCA 
GAGGTGGAAA GAAACCGAAG TCTGACACAG TCACGAACTG ATGCTGAGGT GCACGAACAC 
CAACTAAATA CAAAATCGGC CTTGAAATGC CCTGGCTTGG GGGCTGTCAT CCCTGGTCAT 
CTCTGTCCTC GAAACAGCTA CAGTAGCAGC AGTGAACTGT CTCTTTCAAG CACCTGCAGC 
GAGTACTCCA GTGGCTCCTC CTACACGTGG CACGATGGGA AGAACCTCAG GAAAAGGCAA 
TCATCACAAA ACTGGGATAA AAGGCTAAGT ATTGATTCTT CGCTCCCAAG TGGCTTTGCT 
AGTCCTACAA ATGAACTACC TCCAACTCGT ATCAAGGAAA GCCACATTTT GGAAGGGCTA 
AGAAAGCTAC AGAAGCGAAA AGTGTTACTT GAACCCCCAT CAGTGATAAC CAAATGGGGT 
TATAAAGATT GCATGAACTC GAATGAAGGA ATATATTCTC CTGGAATTAA AAGTAGCAGC 
CTCAAGGAGT ATCCCCCCTG CAAAACAGCT GACCTGGGGA GCCCCTGCAA GGAACCCCAC 
AAGACATTTG TTTATGATCT AGATTCCCAC GTTGATGCGG ACGATGACCC TTCCACCTTA 
GCATTGCTCC AAGCAGTTCC AAACCAGAGC TGCAGGCCAC ATGGCAGTAA ATTAACCCAC 
AGTGTTTCCG ACAGTCTGTT TGGCTGGGAG ACAAACAGAA AACACTTTCT GGAAGGCACA 
TCCTCAGTTT ATCCCAAGGA AAGGCCTGAA AAGCTGACAA GTTGCGCCAG CAGCTGTCCC 
TTAGAGATGA AGCTGTGCCC AAGTGTGCAG ACGCCTCAGG TACAGAGGGA GAGGGGCCCA 
CAGGGCCAAG GCCATGGCCG CATGGCTCTC AACCTCCAGC TTTCAGACAC TGATGACAAT 
GAAACGTTTG ATGAGCTGCA CATAGAGAGC AGTGATGAGA AAAGTCCTTC AGACGTGTCA 
TTGGCTGCCG ACACCGATAA GTCCGTGGAG AACCTGGATG TCCTTGTGGG GTTTGGAAAA 
TCTCTATGTG GGTCTCCTGA AGAGGAGGAA AAACAAGTGC CCATCCCTTC AGAGACTAGG 
CCAAAGACTT TTAGTTTCAT TAAGCAGCAA AGAGTTGTAA AAAGGACTTC TTCAGAAGAA 
TGTGTGACTG TGATATTTGA TGCGGAAGAC GGTGAGCCCA TTGAATTCAG CTCTCACCAG 
ACTGGGGTTG TCACTGTTAC CAGAAATGAG ATTTCCATCA ATTCAACTCC TGCTGGACCC 
AAGGCAGAAC ATACTGAGCT TTTACCTCAG GGAATTGCTT GTTTACAGCC AAGAGCTGCT 
GCAAGAGACT ATACTTTCTT TAAAAGGTCT GAAGAGGACA CTGAGAAAAA CATTCCAAAA 
GATAATGTAG ATAATGTTCC CAGGGTGTCC ACTGAATCTT TCAGCTCCAG GACAGTGACA 
CAAAATCCTC AGCAGCAAAA GCTGGTCAAA CCAACACACA ATATATCATG CCAGAGTAAT 
TCCAGGTCTT CGGCACCCAT GGGCATCTAT CAAAAGCAAA ATCTGACAAA AATACCTCCC 
AGGGGCAAGT CTTCACCTCA GAAATCAAAA CTAATGGAGC CCGAAGCCAC CACACTACTC 
CCTTCATCTG GCCTGGTGAC TCTTGAAAAA TCACCAGCCT TAGCTCCTGG GAAACTCTCA 
CGATTCATGA AGACTGAGAG CTCAGGGCCC CTCTTTGAAT TACGATCAGA TCCACACATT 
CCAAAACATT CCGCCCAACT TCCGCACAGC TCCAGGATGC CCAGCAGGAG GGACTGGGTC 
CAGTGCCCCA AGAGTCAGAC TCCAGGGTCA CGGTCAAGGC CTGCCATTGA GTCTAGTGAC 
AGTGGAGAGC CCCCCACGAG GGATGAACAC TGTGGCTCTG GGCCGGAGGC AGGGGTGAAA 
TCCCCTTCCC CTCCGCCCCC TCCAGGCAGG TCCGTCTCCC TGCTGGCCAG GCCCAGCTAT 
GACTATTCAC CAGCACCTTC ATCCACCAAG TCCGAAACCA GGGTCCCCAG TGAAACAGCA 
AGGACCCCAT TCAAATCCCC GCTGCTGAAA GGAATTTCTG CTCCAGTTAT TTCTTCTAAT 
CCGGCCACGA CAGAAGTGCA GAGGAAGAAA CCTTCTGTGG CCTTCAAAAA GCCTATCTTC 
ACTCACCCTA TGCCCTCCCC AGAAGCAGTC ATTCAAACCC GATGCCCTGC TCATGCCCCC 
TCCAGCTCCT TCACCGTAAT GGCTCTGGGG CCTCCAAAGG TCTCTCCGAA GAGAGGTGTC 
CCCAAAACCT CTCCTCGCCA AACACTTGGG ACCCCACAAA GGGACATAGG ATTACAGACT 
CCCAGGATCT CTCCTTCAAC CCATGAGCCA CTGGAAATGA CGTCCTCCAA AAGTGTATCT 
CCAGGGAGAA AAGGACAATT GAATGATAGC GCCTCCACAC CCCCCAAGCC TTCCTTCTTA 
GGGGTAAATG AGTCACCATC ATCTCAGGTC AGCAGTTCCT CATCATCCTC ATCACCCGCC 
AAAAGCCATA ACAGCCCTCA TGGTTGTCAA AGTGCTCATG AGAAAGGACT GAAAACTCGC 
CTTCCAGTGG GACTCAAAGT GCTCATGAAG TCTCCCCAGC TGCTCAGGAA AAGTTCCACC 
GTGCCAGGGA AACATGAAAA AGACAGTTTA AATGAAGCCT CCAAAAGTTC CGTGGCTGTG 
AACAAGTCTA AGCCAGAGGA CTCCAAGAAT CCAGCAAGCA TGGAGATCAC AGCGGGTGAA 
AGAAATGTGA CCCTACCGGA TTCACAAGCA CAGGGCAGTT TAGCTGATGG GCTTCCCCTG 
GAAACAGCAC TACAAGAGCC ATTGGAAAGT AGCATCCCTG GGAGTGATGG AAGGGATGGG 
GTAGATAATA GATCCATGAG AAGATCCCTT TCCTCCAGCA AACCACACCT AAAACCAGCT 
CTGGGTATGA ATGGCGCCAA AGCCCGCAGC CACAGCTTCA GTACACACTC AGGAGACAAG 
CCTTCTACGC CCCCCATCGA AGGGTCAGGC AAAGTCCGCA CTCAGATCAT TACCAATACC 
GCCGAGAGAG GCAATTCTCT TACCCGGCAG AACTCTTCCA CGGAAAGCTC TCCCAACAAG 
GCCCCTTCTG CTCCCATGTT GGAGAGTCTC CCCAGTGTTG GGAGGCCCTC GGGGCACCCC 
TCCTCCGGGA AGGGCTCCCT GGGGAGCTCA GGCAGCTTCA GCAGTCAGCA TGGGAGCCCA 
AGTAAGTTGC CTTTGAGGAT CCCTCCAAAG TCTGAGGGAC TCCTCATCCC ACCTGGAAAG 
GAAGACCAGC AGGCCTTCAC CCAGGGAGAG TGCCCCAGTG CCAATGTGGC TGTACTTGGG 
GAACCAGGCA GTGACCGCCG CAGTTGCCCA CCCACCCCAA CAGACTGCCC TGAAGCCCTG 
CAGAGCCCAG GGAGGACTCA GCATCCAAGC ACTTTTGAAA CAAGCAGTAC ATCCAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCCTCT GCAACCGCGA CTGATGCTGT GAGTTCAGAA 
GCCCCCCTCT CACCCACAAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA 
AAGGGCCAAG TGCAAACAAA GCCCACCTCT GTGGAAGCAA AGCAGAAGCC TGGGCCTTCT 
TTTGCCAGCT GGTTTGGTTT TCGGAAGAGT AGACTTCCAG CTCTGAGTAG CAGGAAAATG 
GACATCTCCA AAACCAAAGT AGAAAAGAAA GATGCAAAAG TCTTGGGGTT TGGAAACAGA 
CAACTAAAAT CAGAAAGAAA AAAAGAGAAA AAGAAGCCTG AACTACAGTG TGAGACAGAA 
AATGAGCTTA TCAAGGACAC CAAGTCAGCA GATAATCCAG ATGGCGGTTT ACAAAGCAAA 
AATAATCGGA GAACACCACA AGACATTTAC AACCAACTGA AGATTGAACC AAGGAATAGA 
CACAGCCCTG TTGCATGTTC AACGAAAGAC ACCTTCATGA CGGAACTCTT GAACAGAGTT 
GATAAGAAAG CAGCTCCACA GACAGAAAGT GGATCAAGTA ATGCTTCCTG CAGGAATGTG 
TTAAAGGGCA GTTCTCAGGG CTCCTGTCTC ATCGGCAGCT CTATCAGTAC TCAAGGAAAC 
CACAAGAAAA ACATGAAAAT CAAAGCCGAT ATGGAAGTAC CAAAAGACTC CCTGGTAAAA 
GAGGCAAATG AAAACTTGCA AGAGGATGAA GACGATGCAG TTGCAGATTC TGTATTTCAG 
AGCCACATCA TAGAATCCAA CTGCCAGATG AGAACATTGG ACAGTGGGAT CGGAACCTTT 
CCACTCCCAG ACTCGGGAAA TCGCTCGACA GGACGCTACC TATGCCAGCC AGACTCCCCA 
GAGGACGCTG AGCCTCTCCT GCCTCTCCAG TCAGCCCTTT CTGCAGTTTC TTCCATGAGA 
GCCCAAACCC TTGAACGTGA AGTGCCTTCC TCCACAGACG GCCAGCGCCC TGCAGATAGC 
GCCATTGTTC ATTCCACATC CGACCCCATC ATGACCGCCA GAGGGATGAG GCCTCTTCAG 
AGCCGCCTCC CCAAACCAGC TTCCTCAGGA AAAGTCAGTT CCCAAAAGCA GAATGAAGCA 
GAGCCAAGGC CTCAGACATG CTCATCATTC GGATATGCTG AAGACCCAAT GGCAAGCCAG 
CCGCTTCCAG ACTGGGGGAG TGAAGTTGCT GCCACCGGGA CCCAGAAATT GAGACAACTT 
GAAGAAACAA AAGACGATCC CGAGAATAGA TTATCGAAAA TTTCCCTAGA GTCATTCAAT 
AAATTTAACA GCAATACTGT GATTTTATTA GAAAAAGAGA AGAACTCTCT GAACAAGGTT 
GAAGGACAGA AGGAAGAAAA AGAAAAAAAT GAAGAGACAT CTTTGAGTAG TTCAGATAGG 
CCTGGGGTAG ACAACTTGGA ATCTTTGAGT GATTCTTTAT ATGATAGCTT CTCTTCCTGT 
GCCAGTCAAG GTTCAAATGA TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_017003977.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859466.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X12, mRNA.

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CloneID	OHu79436
Clone ID Related Accession (Same CDS sequence)	XM_017003976.2 , XM_011511102.2 , XM_017003977.1 , XM_017003977.2 , XM_017003976.1 , XM_011511101.1
Accession Version	XM_017003977.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA ATAAATACAT TGAGCATCTG CTGACTCAGC TTGAGGAGCA ACACAGGAGT 
CTCTGGAGGG AGAAGTTGGC AGTGGCCCGA CTACAGCGAG AAGTTGCCCA AAGAACAAGT 
GAGGGGGCCA TGCATGAGAA GCTGATACAT GAACTGGAAG AGGAGAGACA CTTACGTCTT 
CAAAGCGAGA AGCGGTTGCA GGAGGTGACC CTAGAGTCTG AACGCAACAG AATTCAGATG 
CGTAGCTTGC AGCAGCAGTT CTCCAGGATG GAAGAAACAG TACGAAATCT ATTGCAGAGT 
CAAGGATCTC CAGAGCAGAA AAAAGAAGAA ACTGTTAATA TAATGGTCTA TCAGGAAAAG 
CTGTCAGAGG AAGAGAGAAA ACATAAGGAA GCTTTGGAAG ATCTTCACAT GGTGGTTGAT 
GAGGATTCGA GGAGTGAAAG CAGCAGTACA GATGAGGGAA AAGAAAAGAC CAAATTGCTA 
TTAGAGAGAT TGAAAGCTCT AGAGGCAGAG AATTCAGCGT TGGCTTTGGA GAATGAAAAT 
CAAAGGGAAC AATATGAGCG ATGTCTTGAT GAGGTAGCCA ACCAAGTTGT TCAAGCTCTG 
CTTACTCAAA AGGATCTAAG AGAGGAATGT GTGAAGTTGA AAACAAGAGT GTTTGATTTG 
GAACAGCAGA ATCGAACACT AAGCATCCTA TTCCAACAGC GAGTCAGACC CACTTCTGAT 
CTGCTCCTCC AGAAACTTCA CTCACGTCTC TTGGATCTTT CATCTGGAGA TTTGCTTTCA 
GAGGTGGAAA GAAACCGAAG TCTGACACAG TCACGAACTG ATGCTGAGGT GCACGAACAC 
CAACTAAATA CAAAATCGGC CTTGAAATGC CCTGGCTTGG GGGCTGTCAT CCCTGGTCAT 
CTCTGTCCTC GAAACAGCTA CAGTAGCAGC AGTGAACTGT CTCTTTCAAG CACCTGCAGC 
GAGTACTCCA GTGGCTCCTC CTACACGTGG CACGATGGGA AGAACCTCAG GAAAAGGCAA 
TCATCACAAA ACTGGGATAA AAGGCTAAGT ATTGATTCTT CGCTCCCAAG TGGCTTTGCT 
AGTCCTACAA ATGAACTACC TCCAACTCGT ATCAAGGAAA GCCACATTTT GGAAGGGCTA 
AGAAAGCTAC AGAAGCGAAA AGTGTTACTT GAACCCCCAT CAGTGATAAC CAAATGGGGT 
TATAAAGATT GCATGAACTC GAATGAAGGA ATATATTCTC CTGGAATTAA AAGTAGCAGC 
CTCAAGGAGT ATCCCCCCTG CAAAACAGCT GACCTGGGGA GCCCCTGCAA GGAACCCCAC 
AAGACATTTG TTTATGATCT AGATTCCCAC GTTGATGCGG ACGATGACCC TTCCACCTTA 
GCATTGCTCC AAGCAGTTCC AAACCAGAGC TGCAGGCCAC ATGGCAGTAA ATTAACCCAC 
AGTGTTTCCG ACAGTCTGTT TGGCTGGGAG ACAAACAGAA AACACTTTCT GGAAGGCACA 
TCCTCAGTTT ATCCCAAGGA AAGGCCTGAA AAGCTGACAA GTTGCGCCAG CAGCTGTCCC 
TTAGAGATGA AGCTGTGCCC AAGTGTGCAG ACGCCTCAGG TACAGAGGGA GAGGGGCCCA 
CAGGGCCAAG GCCATGGCCG CATGGCTCTC AACCTCCAGC TTTCAGACAC TGATGACAAT 
GAAACGTTTG ATGAGCTGCA CATAGAGAGC AGTGATGAGA AAAGTCCTTC AGACGTGTCA 
TTGGCTGCCG ACACCGATAA GTCCGTGGAG AACCTGGATG TCCTTGTGGG GTTTGGAAAA 
TCTCTATGTG GGTCTCCTGA AGAGGAGGAA AAACAAGTGC CCATCCCTTC AGAGACTAGG 
CCAAAGACTT TTAGTTTCAT TAAGCAGCAA AGAGTTGTAA AAAGGACTTC TTCAGAAGAA 
TGTGTGACTG TGATATTTGA TGCGGAAGAC GGTGAGCCCA TTGAATTCAG CTCTCACCAG 
ACTGGGGTTG TCACTGTTAC CAGAAATGAG ATTTCCATCA ATTCAACTCC TGCTGGACCC 
AAGGCAGAAC ATACTGAGCT TTTACCTCAG GGAATTGCTT GTTTACAGCC AAGAGCTGCT 
GCAAGAGACT ATACTTTCTT TAAAAGGTCT GAAGAGGACA CTGAGAAAAA CATTCCAAAA 
GATAATGTAG ATAATGTTCC CAGGGTGTCC ACTGAATCTT TCAGCTCCAG GACAGTGACA 
CAAAATCCTC AGCAGCAAAA GCTGGTCAAA CCAACACACA ATATATCATG CCAGAGTAAT 
TCCAGGTCTT CGGCACCCAT GGGCATCTAT CAAAAGCAAA ATCTGACAAA AATACCTCCC 
AGGGGCAAGT CTTCACCTCA GAAATCAAAA CTAATGGAGC CCGAAGCCAC CACACTACTC 
CCTTCATCTG GCCTGGTGAC TCTTGAAAAA TCACCAGCCT TAGCTCCTGG GAAACTCTCA 
CGATTCATGA AGACTGAGAG CTCAGGGCCC CTCTTTGAAT TACGATCAGA TCCACACATT 
CCAAAACATT CCGCCCAACT TCCGCACAGC TCCAGGATGC CCAGCAGGAG GGACTGGGTC 
CAGTGCCCCA AGAGTCAGAC TCCAGGGTCA CGGTCAAGGC CTGCCATTGA GTCTAGTGAC 
AGTGGAGAGC CCCCCACGAG GGATGAACAC TGTGGCTCTG GGCCGGAGGC AGGGGTGAAA 
TCCCCTTCCC CTCCGCCCCC TCCAGGCAGG TCCGTCTCCC TGCTGGCCAG GCCCAGCTAT 
GACTATTCAC CAGCACCTTC ATCCACCAAG TCCGAAACCA GGGTCCCCAG TGAAACAGCA 
AGGACCCCAT TCAAATCCCC GCTGCTGAAA GGAATTTCTG CTCCAGTTAT TTCTTCTAAT 
CCGGCCACGA CAGAAGTGCA GAGGAAGAAA CCTTCTGTGG CCTTCAAAAA GCCTATCTTC 
ACTCACCCTA TGCCCTCCCC AGAAGCAGTC ATTCAAACCC GATGCCCTGC TCATGCCCCC 
TCCAGCTCCT TCACCGTAAT GGCTCTGGGG CCTCCAAAGG TCTCTCCGAA GAGAGGTGTC 
CCCAAAACCT CTCCTCGCCA AACACTTGGG ACCCCACAAA GGGACATAGG ATTACAGACT 
CCCAGGATCT CTCCTTCAAC CCATGAGCCA CTGGAAATGA CGTCCTCCAA AAGTGTATCT 
CCAGGGAGAA AAGGACAATT GAATGATAGC GCCTCCACAC CCCCCAAGCC TTCCTTCTTA 
GGGGTAAATG AGTCACCATC ATCTCAGGTC AGCAGTTCCT CATCATCCTC ATCACCCGCC 
AAAAGCCATA ACAGCCCTCA TGGTTGTCAA AGTGCTCATG AGAAAGGACT GAAAACTCGC 
CTTCCAGTGG GACTCAAAGT GCTCATGAAG TCTCCCCAGC TGCTCAGGAA AAGTTCCACC 
GTGCCAGGGA AACATGAAAA AGACAGTTTA AATGAAGCCT CCAAAAGTTC CGTGGCTGTG 
AACAAGTCTA AGCCAGAGGA CTCCAAGAAT CCAGCAAGCA TGGAGATCAC AGCGGGTGAA 
AGAAATGTGA CCCTACCGGA TTCACAAGCA CAGGGCAGTT TAGCTGATGG GCTTCCCCTG 
GAAACAGCAC TACAAGAGCC ATTGGAAAGT AGCATCCCTG GGAGTGATGG AAGGGATGGG 
GTAGATAATA GATCCATGAG AAGATCCCTT TCCTCCAGCA AACCACACCT AAAACCAGCT 
CTGGGTATGA ATGGCGCCAA AGCCCGCAGC CACAGCTTCA GTACACACTC AGGAGACAAG 
CCTTCTACGC CCCCCATCGA AGGGTCAGGC AAAGTCCGCA CTCAGATCAT TACCAATACC 
GCCGAGAGAG GCAATTCTCT TACCCGGCAG AACTCTTCCA CGGAAAGCTC TCCCAACAAG 
GCCCCTTCTG CTCCCATGTT GGAGAGTCTC CCCAGTGTTG GGAGGCCCTC GGGGCACCCC 
TCCTCCGGGA AGGGCTCCCT GGGGAGCTCA GGCAGCTTCA GCAGTCAGCA TGGGAGCCCA 
AGTAAGTTGC CTTTGAGGAT CCCTCCAAAG TCTGAGGGAC TCCTCATCCC ACCTGGAAAG 
GAAGACCAGC AGGCCTTCAC CCAGGGAGAG TGCCCCAGTG CCAATGTGGC TGTACTTGGG 
GAACCAGGCA GTGACCGCCG CAGTTGCCCA CCCACCCCAA CAGACTGCCC TGAAGCCCTG 
CAGAGCCCAG GGAGGACTCA GCATCCAAGC ACTTTTGAAA CAAGCAGTAC ATCCAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCCTCT GCAACCGCGA CTGATGCTGT GAGTTCAGAA 
GCCCCCCTCT CACCCACAAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA 
AAGGGCCAAG TGCAAACAAA GCCCACCTCT GTGGAAGCAA AGCAGAAGCC TGGGCCTTCT 
TTTGCCAGCT GGTTTGGTTT TCGGAAGAGT AGACTTCCAG CTCTGAGTAG CAGGAAAATG 
GACATCTCCA AAACCAAAGT AGAAAAGAAA GATGCAAAAG TCTTGGGGTT TGGAAACAGA 
CAACTAAAAT CAGAAAGAAA AAAAGAGAAA AAGAAGCCTG AACTACAGTG TGAGACAGAA 
AATGAGCTTA TCAAGGACAC CAAGTCAGCA GATAATCCAG ATGGCGGTTT ACAAAGCAAA 
AATAATCGGA GAACACCACA AGACATTTAC AACCAACTGA AGATTGAACC AAGGAATAGA 
CACAGCCCTG TTGCATGTTC AACGAAAGAC ACCTTCATGA CGGAACTCTT GAACAGAGTT 
GATAAGAAAG CAGCTCCACA GACAGAAAGT GGATCAAGTA ATGCTTCCTG CAGGAATGTG 
TTAAAGGGCA GTTCTCAGGG CTCCTGTCTC ATCGGCAGCT CTATCAGTAC TCAAGGAAAC 
CACAAGAAAA ACATGAAAAT CAAAGCCGAT ATGGAAGTAC CAAAAGACTC CCTGGTAAAA 
GAGGCAAATG AAAACTTGCA AGAGGATGAA GACGATGCAG TTGCAGATTC TGTATTTCAG 
AGCCACATCA TAGAATCCAA CTGCCAGATG AGAACATTGG ACAGTGGGAT CGGAACCTTT 
CCACTCCCAG ACTCGGGAAA TCGCTCGACA GGACGCTACC TATGCCAGCC AGACTCCCCA 
GAGGACGCTG AGCCTCTCCT GCCTCTCCAG TCAGCCCTTT CTGCAGTTTC TTCCATGAGA 
GCCCAAACCC TTGAACGTGA AGTGCCTTCC TCCACAGACG GCCAGCGCCC TGCAGATAGC 
GCCATTGTTC ATTCCACATC CGACCCCATC ATGACCGCCA GAGGGATGAG GCCTCTTCAG 
AGCCGCCTCC CCAAACCAGC TTCCTCAGGA AAAGTCAGTT CCCAAAAGCA GAATGAAGCA 
GAGCCAAGGC CTCAGACATG CTCATCATTC GGATATGCTG AAGACCCAAT GGCAAGCCAG 
CCGCTTCCAG ACTGGGGGAG TGAAGTTGCT GCCACCGGGA CCCAGAAATT GAGACAACTT 
GAAGAAACAA AAGACGATCC CGAGAATAGA TTATCGAAAA TTTCCCTAGA GTCATTCAAT 
AAATTTAACA GCAATACTGT GATTTTATTA GAAAAAGAGA AGAACTCTCT GAACAAGGTT 
GAAGGACAGA AGGAAGAAAA AGAAAAAAAT GAAGAGACAT CTTTGAGTAG TTCAGATAGG 
CCTGGGGTAG ACAACTTGGA ATCTTTGAGT GATTCTTTAT ATGATAGCTT CTCTTCCTGT 
GCCAGTCAAG GTTCAAATGA TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859466.1
CDS	1151..6937
Translation	MDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_017003977.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003977.1

ORF Insert Sequence:

ATGGACTCCA ATAAATACAT TGAGCATCTG CTGACTCAGC TTGAGGAGCA ACACAGGAGT 
CTCTGGAGGG AGAAGTTGGC AGTGGCCCGA CTACAGCGAG AAGTTGCCCA AAGAACAAGT 
GAGGGGGCCA TGCATGAGAA GCTGATACAT GAACTGGAAG AGGAGAGACA CTTACGTCTT 
CAAAGCGAGA AGCGGTTGCA GGAGGTGACC CTAGAGTCTG AACGCAACAG AATTCAGATG 
CGTAGCTTGC AGCAGCAGTT CTCCAGGATG GAAGAAACAG TACGAAATCT ATTGCAGAGT 
CAAGGATCTC CAGAGCAGAA AAAAGAAGAA ACTGTTAATA TAATGGTCTA TCAGGAAAAG 
CTGTCAGAGG AAGAGAGAAA ACATAAGGAA GCTTTGGAAG ATCTTCACAT GGTGGTTGAT 
GAGGATTCGA GGAGTGAAAG CAGCAGTACA GATGAGGGAA AAGAAAAGAC CAAATTGCTA 
TTAGAGAGAT TGAAAGCTCT AGAGGCAGAG AATTCAGCGT TGGCTTTGGA GAATGAAAAT 
CAAAGGGAAC AATATGAGCG ATGTCTTGAT GAGGTAGCCA ACCAAGTTGT TCAAGCTCTG 
CTTACTCAAA AGGATCTAAG AGAGGAATGT GTGAAGTTGA AAACAAGAGT GTTTGATTTG 
GAACAGCAGA ATCGAACACT AAGCATCCTA TTCCAACAGC GAGTCAGACC CACTTCTGAT 
CTGCTCCTCC AGAAACTTCA CTCACGTCTC TTGGATCTTT CATCTGGAGA TTTGCTTTCA 
GAGGTGGAAA GAAACCGAAG TCTGACACAG TCACGAACTG ATGCTGAGGT GCACGAACAC 
CAACTAAATA CAAAATCGGC CTTGAAATGC CCTGGCTTGG GGGCTGTCAT CCCTGGTCAT 
CTCTGTCCTC GAAACAGCTA CAGTAGCAGC AGTGAACTGT CTCTTTCAAG CACCTGCAGC 
GAGTACTCCA GTGGCTCCTC CTACACGTGG CACGATGGGA AGAACCTCAG GAAAAGGCAA 
TCATCACAAA ACTGGGATAA AAGGCTAAGT ATTGATTCTT CGCTCCCAAG TGGCTTTGCT 
AGTCCTACAA ATGAACTACC TCCAACTCGT ATCAAGGAAA GCCACATTTT GGAAGGGCTA 
AGAAAGCTAC AGAAGCGAAA AGTGTTACTT GAACCCCCAT CAGTGATAAC CAAATGGGGT 
TATAAAGATT GCATGAACTC GAATGAAGGA ATATATTCTC CTGGAATTAA AAGTAGCAGC 
CTCAAGGAGT ATCCCCCCTG CAAAACAGCT GACCTGGGGA GCCCCTGCAA GGAACCCCAC 
AAGACATTTG TTTATGATCT AGATTCCCAC GTTGATGCGG ACGATGACCC TTCCACCTTA 
GCATTGCTCC AAGCAGTTCC AAACCAGAGC TGCAGGCCAC ATGGCAGTAA ATTAACCCAC 
AGTGTTTCCG ACAGTCTGTT TGGCTGGGAG ACAAACAGAA AACACTTTCT GGAAGGCACA 
TCCTCAGTTT ATCCCAAGGA AAGGCCTGAA AAGCTGACAA GTTGCGCCAG CAGCTGTCCC 
TTAGAGATGA AGCTGTGCCC AAGTGTGCAG ACGCCTCAGG TACAGAGGGA GAGGGGCCCA 
CAGGGCCAAG GCCATGGCCG CATGGCTCTC AACCTCCAGC TTTCAGACAC TGATGACAAT 
GAAACGTTTG ATGAGCTGCA CATAGAGAGC AGTGATGAGA AAAGTCCTTC AGACGTGTCA 
TTGGCTGCCG ACACCGATAA GTCCGTGGAG AACCTGGATG TCCTTGTGGG GTTTGGAAAA 
TCTCTATGTG GGTCTCCTGA AGAGGAGGAA AAACAAGTGC CCATCCCTTC AGAGACTAGG 
CCAAAGACTT TTAGTTTCAT TAAGCAGCAA AGAGTTGTAA AAAGGACTTC TTCAGAAGAA 
TGTGTGACTG TGATATTTGA TGCGGAAGAC GGTGAGCCCA TTGAATTCAG CTCTCACCAG 
ACTGGGGTTG TCACTGTTAC CAGAAATGAG ATTTCCATCA ATTCAACTCC TGCTGGACCC 
AAGGCAGAAC ATACTGAGCT TTTACCTCAG GGAATTGCTT GTTTACAGCC AAGAGCTGCT 
GCAAGAGACT ATACTTTCTT TAAAAGGTCT GAAGAGGACA CTGAGAAAAA CATTCCAAAA 
GATAATGTAG ATAATGTTCC CAGGGTGTCC ACTGAATCTT TCAGCTCCAG GACAGTGACA 
CAAAATCCTC AGCAGCAAAA GCTGGTCAAA CCAACACACA ATATATCATG CCAGAGTAAT 
TCCAGGTCTT CGGCACCCAT GGGCATCTAT CAAAAGCAAA ATCTGACAAA AATACCTCCC 
AGGGGCAAGT CTTCACCTCA GAAATCAAAA CTAATGGAGC CCGAAGCCAC CACACTACTC 
CCTTCATCTG GCCTGGTGAC TCTTGAAAAA TCACCAGCCT TAGCTCCTGG GAAACTCTCA 
CGATTCATGA AGACTGAGAG CTCAGGGCCC CTCTTTGAAT TACGATCAGA TCCACACATT 
CCAAAACATT CCGCCCAACT TCCGCACAGC TCCAGGATGC CCAGCAGGAG GGACTGGGTC 
CAGTGCCCCA AGAGTCAGAC TCCAGGGTCA CGGTCAAGGC CTGCCATTGA GTCTAGTGAC 
AGTGGAGAGC CCCCCACGAG GGATGAACAC TGTGGCTCTG GGCCGGAGGC AGGGGTGAAA 
TCCCCTTCCC CTCCGCCCCC TCCAGGCAGG TCCGTCTCCC TGCTGGCCAG GCCCAGCTAT 
GACTATTCAC CAGCACCTTC ATCCACCAAG TCCGAAACCA GGGTCCCCAG TGAAACAGCA 
AGGACCCCAT TCAAATCCCC GCTGCTGAAA GGAATTTCTG CTCCAGTTAT TTCTTCTAAT 
CCGGCCACGA CAGAAGTGCA GAGGAAGAAA CCTTCTGTGG CCTTCAAAAA GCCTATCTTC 
ACTCACCCTA TGCCCTCCCC AGAAGCAGTC ATTCAAACCC GATGCCCTGC TCATGCCCCC 
TCCAGCTCCT TCACCGTAAT GGCTCTGGGG CCTCCAAAGG TCTCTCCGAA GAGAGGTGTC 
CCCAAAACCT CTCCTCGCCA AACACTTGGG ACCCCACAAA GGGACATAGG ATTACAGACT 
CCCAGGATCT CTCCTTCAAC CCATGAGCCA CTGGAAATGA CGTCCTCCAA AAGTGTATCT 
CCAGGGAGAA AAGGACAATT GAATGATAGC GCCTCCACAC CCCCCAAGCC TTCCTTCTTA 
GGGGTAAATG AGTCACCATC ATCTCAGGTC AGCAGTTCCT CATCATCCTC ATCACCCGCC 
AAAAGCCATA ACAGCCCTCA TGGTTGTCAA AGTGCTCATG AGAAAGGACT GAAAACTCGC 
CTTCCAGTGG GACTCAAAGT GCTCATGAAG TCTCCCCAGC TGCTCAGGAA AAGTTCCACC 
GTGCCAGGGA AACATGAAAA AGACAGTTTA AATGAAGCCT CCAAAAGTTC CGTGGCTGTG 
AACAAGTCTA AGCCAGAGGA CTCCAAGAAT CCAGCAAGCA TGGAGATCAC AGCGGGTGAA 
AGAAATGTGA CCCTACCGGA TTCACAAGCA CAGGGCAGTT TAGCTGATGG GCTTCCCCTG 
GAAACAGCAC TACAAGAGCC ATTGGAAAGT AGCATCCCTG GGAGTGATGG AAGGGATGGG 
GTAGATAATA GATCCATGAG AAGATCCCTT TCCTCCAGCA AACCACACCT AAAACCAGCT 
CTGGGTATGA ATGGCGCCAA AGCCCGCAGC CACAGCTTCA GTACACACTC AGGAGACAAG 
CCTTCTACGC CCCCCATCGA AGGGTCAGGC AAAGTCCGCA CTCAGATCAT TACCAATACC 
GCCGAGAGAG GCAATTCTCT TACCCGGCAG AACTCTTCCA CGGAAAGCTC TCCCAACAAG 
GCCCCTTCTG CTCCCATGTT GGAGAGTCTC CCCAGTGTTG GGAGGCCCTC GGGGCACCCC 
TCCTCCGGGA AGGGCTCCCT GGGGAGCTCA GGCAGCTTCA GCAGTCAGCA TGGGAGCCCA 
AGTAAGTTGC CTTTGAGGAT CCCTCCAAAG TCTGAGGGAC TCCTCATCCC ACCTGGAAAG 
GAAGACCAGC AGGCCTTCAC CCAGGGAGAG TGCCCCAGTG CCAATGTGGC TGTACTTGGG 
GAACCAGGCA GTGACCGCCG CAGTTGCCCA CCCACCCCAA CAGACTGCCC TGAAGCCCTG 
CAGAGCCCAG GGAGGACTCA GCATCCAAGC ACTTTTGAAA CAAGCAGTAC ATCCAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCCTCT GCAACCGCGA CTGATGCTGT GAGTTCAGAA 
GCCCCCCTCT CACCCACAAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA 
AAGGGCCAAG TGCAAACAAA GCCCACCTCT GTGGAAGCAA AGCAGAAGCC TGGGCCTTCT 
TTTGCCAGCT GGTTTGGTTT TCGGAAGAGT AGACTTCCAG CTCTGAGTAG CAGGAAAATG 
GACATCTCCA AAACCAAAGT AGAAAAGAAA GATGCAAAAG TCTTGGGGTT TGGAAACAGA 
CAACTAAAAT CAGAAAGAAA AAAAGAGAAA AAGAAGCCTG AACTACAGTG TGAGACAGAA 
AATGAGCTTA TCAAGGACAC CAAGTCAGCA GATAATCCAG ATGGCGGTTT ACAAAGCAAA 
AATAATCGGA GAACACCACA AGACATTTAC AACCAACTGA AGATTGAACC AAGGAATAGA 
CACAGCCCTG TTGCATGTTC AACGAAAGAC ACCTTCATGA CGGAACTCTT GAACAGAGTT 
GATAAGAAAG CAGCTCCACA GACAGAAAGT GGATCAAGTA ATGCTTCCTG CAGGAATGTG 
TTAAAGGGCA GTTCTCAGGG CTCCTGTCTC ATCGGCAGCT CTATCAGTAC TCAAGGAAAC 
CACAAGAAAA ACATGAAAAT CAAAGCCGAT ATGGAAGTAC CAAAAGACTC CCTGGTAAAA 
GAGGCAAATG AAAACTTGCA AGAGGATGAA GACGATGCAG TTGCAGATTC TGTATTTCAG 
AGCCACATCA TAGAATCCAA CTGCCAGATG AGAACATTGG ACAGTGGGAT CGGAACCTTT 
CCACTCCCAG ACTCGGGAAA TCGCTCGACA GGACGCTACC TATGCCAGCC AGACTCCCCA 
GAGGACGCTG AGCCTCTCCT GCCTCTCCAG TCAGCCCTTT CTGCAGTTTC TTCCATGAGA 
GCCCAAACCC TTGAACGTGA AGTGCCTTCC TCCACAGACG GCCAGCGCCC TGCAGATAGC 
GCCATTGTTC ATTCCACATC CGACCCCATC ATGACCGCCA GAGGGATGAG GCCTCTTCAG 
AGCCGCCTCC CCAAACCAGC TTCCTCAGGA AAAGTCAGTT CCCAAAAGCA GAATGAAGCA 
GAGCCAAGGC CTCAGACATG CTCATCATTC GGATATGCTG AAGACCCAAT GGCAAGCCAG 
CCGCTTCCAG ACTGGGGGAG TGAAGTTGCT GCCACCGGGA CCCAGAAATT GAGACAACTT 
GAAGAAACAA AAGACGATCC CGAGAATAGA TTATCGAAAA TTTCCCTAGA GTCATTCAAT 
AAATTTAACA GCAATACTGT GATTTTATTA GAAAAAGAGA AGAACTCTCT GAACAAGGTT 
GAAGGACAGA AGGAAGAAAA AGAAAAAAAT GAAGAGACAT CTTTGAGTAG TTCAGATAGG 
CCTGGGGTAG ACAACTTGGA ATCTTTGAGT GATTCTTTAT ATGATAGCTT CTCTTCCTGT 
GCCAGTCAAG GTTCAAATGA TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_017003977.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859466.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X11, mRNA.

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CloneID	OHu79436
Clone ID Related Accession (Same CDS sequence)	XM_017003976.2 , XM_011511102.2 , XM_017003977.1 , XM_017003977.2 , XM_017003976.1 , XM_011511101.1
Accession Version	XM_017003977.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017003977.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA ATAAATACAT TGAGCATCTG CTGACTCAGC TTGAGGAGCA ACACAGGAGT 
CTCTGGAGGG AGAAGTTGGC AGTGGCCCGA CTACAGCGAG AAGTTGCCCA AAGAACAAGT 
GAGGGGGCCA TGCATGAGAA GCTGATACAT GAACTGGAAG AGGAGAGACA CTTACGTCTT 
CAAAGCGAGA AGCGGTTGCA GGAGGTGACC CTAGAGTCTG AACGCAACAG AATTCAGATG 
CGTAGCTTGC AGCAGCAGTT CTCCAGGATG GAAGAAACAG TACGAAATCT ATTGCAGAGT 
CAAGGATCTC CAGAGCAGAA AAAAGAAGAA ACTGTTAATA TAATGGTCTA TCAGGAAAAG 
CTGTCAGAGG AAGAGAGAAA ACATAAGGAA GCTTTGGAAG ATCTTCACAT GGTGGTTGAT 
GAGGATTCGA GGAGTGAAAG CAGCAGTACA GATGAGGGAA AAGAAAAGAC CAAATTGCTA 
TTAGAGAGAT TGAAAGCTCT AGAGGCAGAG AATTCAGCGT TGGCTTTGGA GAATGAAAAT 
CAAAGGGAAC AATATGAGCG ATGTCTTGAT GAGGTAGCCA ACCAAGTTGT TCAAGCTCTG 
CTTACTCAAA AGGATCTAAG AGAGGAATGT GTGAAGTTGA AAACAAGAGT GTTTGATTTG 
GAACAGCAGA ATCGAACACT AAGCATCCTA TTCCAACAGC GAGTCAGACC CACTTCTGAT 
CTGCTCCTCC AGAAACTTCA CTCACGTCTC TTGGATCTTT CATCTGGAGA TTTGCTTTCA 
GAGGTGGAAA GAAACCGAAG TCTGACACAG TCACGAACTG ATGCTGAGGT GCACGAACAC 
CAACTAAATA CAAAATCGGC CTTGAAATGC CCTGGCTTGG GGGCTGTCAT CCCTGGTCAT 
CTCTGTCCTC GAAACAGCTA CAGTAGCAGC AGTGAACTGT CTCTTTCAAG CACCTGCAGC 
GAGTACTCCA GTGGCTCCTC CTACACGTGG CACGATGGGA AGAACCTCAG GAAAAGGCAA 
TCATCACAAA ACTGGGATAA AAGGCTAAGT ATTGATTCTT CGCTCCCAAG TGGCTTTGCT 
AGTCCTACAA ATGAACTACC TCCAACTCGT ATCAAGGAAA GCCACATTTT GGAAGGGCTA 
AGAAAGCTAC AGAAGCGAAA AGTGTTACTT GAACCCCCAT CAGTGATAAC CAAATGGGGT 
TATAAAGATT GCATGAACTC GAATGAAGGA ATATATTCTC CTGGAATTAA AAGTAGCAGC 
CTCAAGGAGT ATCCCCCCTG CAAAACAGCT GACCTGGGGA GCCCCTGCAA GGAACCCCAC 
AAGACATTTG TTTATGATCT AGATTCCCAC GTTGATGCGG ACGATGACCC TTCCACCTTA 
GCATTGCTCC AAGCAGTTCC AAACCAGAGC TGCAGGCCAC ATGGCAGTAA ATTAACCCAC 
AGTGTTTCCG ACAGTCTGTT TGGCTGGGAG ACAAACAGAA AACACTTTCT GGAAGGCACA 
TCCTCAGTTT ATCCCAAGGA AAGGCCTGAA AAGCTGACAA GTTGCGCCAG CAGCTGTCCC 
TTAGAGATGA AGCTGTGCCC AAGTGTGCAG ACGCCTCAGG TACAGAGGGA GAGGGGCCCA 
CAGGGCCAAG GCCATGGCCG CATGGCTCTC AACCTCCAGC TTTCAGACAC TGATGACAAT 
GAAACGTTTG ATGAGCTGCA CATAGAGAGC AGTGATGAGA AAAGTCCTTC AGACGTGTCA 
TTGGCTGCCG ACACCGATAA GTCCGTGGAG AACCTGGATG TCCTTGTGGG GTTTGGAAAA 
TCTCTATGTG GGTCTCCTGA AGAGGAGGAA AAACAAGTGC CCATCCCTTC AGAGACTAGG 
CCAAAGACTT TTAGTTTCAT TAAGCAGCAA AGAGTTGTAA AAAGGACTTC TTCAGAAGAA 
TGTGTGACTG TGATATTTGA TGCGGAAGAC GGTGAGCCCA TTGAATTCAG CTCTCACCAG 
ACTGGGGTTG TCACTGTTAC CAGAAATGAG ATTTCCATCA ATTCAACTCC TGCTGGACCC 
AAGGCAGAAC ATACTGAGCT TTTACCTCAG GGAATTGCTT GTTTACAGCC AAGAGCTGCT 
GCAAGAGACT ATACTTTCTT TAAAAGGTCT GAAGAGGACA CTGAGAAAAA CATTCCAAAA 
GATAATGTAG ATAATGTTCC CAGGGTGTCC ACTGAATCTT TCAGCTCCAG GACAGTGACA 
CAAAATCCTC AGCAGCAAAA GCTGGTCAAA CCAACACACA ATATATCATG CCAGAGTAAT 
TCCAGGTCTT CGGCACCCAT GGGCATCTAT CAAAAGCAAA ATCTGACAAA AATACCTCCC 
AGGGGCAAGT CTTCACCTCA GAAATCAAAA CTAATGGAGC CCGAAGCCAC CACACTACTC 
CCTTCATCTG GCCTGGTGAC TCTTGAAAAA TCACCAGCCT TAGCTCCTGG GAAACTCTCA 
CGATTCATGA AGACTGAGAG CTCAGGGCCC CTCTTTGAAT TACGATCAGA TCCACACATT 
CCAAAACATT CCGCCCAACT TCCGCACAGC TCCAGGATGC CCAGCAGGAG GGACTGGGTC 
CAGTGCCCCA AGAGTCAGAC TCCAGGGTCA CGGTCAAGGC CTGCCATTGA GTCTAGTGAC 
AGTGGAGAGC CCCCCACGAG GGATGAACAC TGTGGCTCTG GGCCGGAGGC AGGGGTGAAA 
TCCCCTTCCC CTCCGCCCCC TCCAGGCAGG TCCGTCTCCC TGCTGGCCAG GCCCAGCTAT 
GACTATTCAC CAGCACCTTC ATCCACCAAG TCCGAAACCA GGGTCCCCAG TGAAACAGCA 
AGGACCCCAT TCAAATCCCC GCTGCTGAAA GGAATTTCTG CTCCAGTTAT TTCTTCTAAT 
CCGGCCACGA CAGAAGTGCA GAGGAAGAAA CCTTCTGTGG CCTTCAAAAA GCCTATCTTC 
ACTCACCCTA TGCCCTCCCC AGAAGCAGTC ATTCAAACCC GATGCCCTGC TCATGCCCCC 
TCCAGCTCCT TCACCGTAAT GGCTCTGGGG CCTCCAAAGG TCTCTCCGAA GAGAGGTGTC 
CCCAAAACCT CTCCTCGCCA AACACTTGGG ACCCCACAAA GGGACATAGG ATTACAGACT 
CCCAGGATCT CTCCTTCAAC CCATGAGCCA CTGGAAATGA CGTCCTCCAA AAGTGTATCT 
CCAGGGAGAA AAGGACAATT GAATGATAGC GCCTCCACAC CCCCCAAGCC TTCCTTCTTA 
GGGGTAAATG AGTCACCATC ATCTCAGGTC AGCAGTTCCT CATCATCCTC ATCACCCGCC 
AAAAGCCATA ACAGCCCTCA TGGTTGTCAA AGTGCTCATG AGAAAGGACT GAAAACTCGC 
CTTCCAGTGG GACTCAAAGT GCTCATGAAG TCTCCCCAGC TGCTCAGGAA AAGTTCCACC 
GTGCCAGGGA AACATGAAAA AGACAGTTTA AATGAAGCCT CCAAAAGTTC CGTGGCTGTG 
AACAAGTCTA AGCCAGAGGA CTCCAAGAAT CCAGCAAGCA TGGAGATCAC AGCGGGTGAA 
AGAAATGTGA CCCTACCGGA TTCACAAGCA CAGGGCAGTT TAGCTGATGG GCTTCCCCTG 
GAAACAGCAC TACAAGAGCC ATTGGAAAGT AGCATCCCTG GGAGTGATGG AAGGGATGGG 
GTAGATAATA GATCCATGAG AAGATCCCTT TCCTCCAGCA AACCACACCT AAAACCAGCT 
CTGGGTATGA ATGGCGCCAA AGCCCGCAGC CACAGCTTCA GTACACACTC AGGAGACAAG 
CCTTCTACGC CCCCCATCGA AGGGTCAGGC AAAGTCCGCA CTCAGATCAT TACCAATACC 
GCCGAGAGAG GCAATTCTCT TACCCGGCAG AACTCTTCCA CGGAAAGCTC TCCCAACAAG 
GCCCCTTCTG CTCCCATGTT GGAGAGTCTC CCCAGTGTTG GGAGGCCCTC GGGGCACCCC 
TCCTCCGGGA AGGGCTCCCT GGGGAGCTCA GGCAGCTTCA GCAGTCAGCA TGGGAGCCCA 
AGTAAGTTGC CTTTGAGGAT CCCTCCAAAG TCTGAGGGAC TCCTCATCCC ACCTGGAAAG 
GAAGACCAGC AGGCCTTCAC CCAGGGAGAG TGCCCCAGTG CCAATGTGGC TGTACTTGGG 
GAACCAGGCA GTGACCGCCG CAGTTGCCCA CCCACCCCAA CAGACTGCCC TGAAGCCCTG 
CAGAGCCCAG GGAGGACTCA GCATCCAAGC ACTTTTGAAA CAAGCAGTAC ATCCAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCCTCT GCAACCGCGA CTGATGCTGT GAGTTCAGAA 
GCCCCCCTCT CACCCACAAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA 
AAGGGCCAAG TGCAAACAAA GCCCACCTCT GTGGAAGCAA AGCAGAAGCC TGGGCCTTCT 
TTTGCCAGCT GGTTTGGTTT TCGGAAGAGT AGACTTCCAG CTCTGAGTAG CAGGAAAATG 
GACATCTCCA AAACCAAAGT AGAAAAGAAA GATGCAAAAG TCTTGGGGTT TGGAAACAGA 
CAACTAAAAT CAGAAAGAAA AAAAGAGAAA AAGAAGCCTG AACTACAGTG TGAGACAGAA 
AATGAGCTTA TCAAGGACAC CAAGTCAGCA GATAATCCAG ATGGCGGTTT ACAAAGCAAA 
AATAATCGGA GAACACCACA AGACATTTAC AACCAACTGA AGATTGAACC AAGGAATAGA 
CACAGCCCTG TTGCATGTTC AACGAAAGAC ACCTTCATGA CGGAACTCTT GAACAGAGTT 
GATAAGAAAG CAGCTCCACA GACAGAAAGT GGATCAAGTA ATGCTTCCTG CAGGAATGTG 
TTAAAGGGCA GTTCTCAGGG CTCCTGTCTC ATCGGCAGCT CTATCAGTAC TCAAGGAAAC 
CACAAGAAAA ACATGAAAAT CAAAGCCGAT ATGGAAGTAC CAAAAGACTC CCTGGTAAAA 
GAGGCAAATG AAAACTTGCA AGAGGATGAA GACGATGCAG TTGCAGATTC TGTATTTCAG 
AGCCACATCA TAGAATCCAA CTGCCAGATG AGAACATTGG ACAGTGGGAT CGGAACCTTT 
CCACTCCCAG ACTCGGGAAA TCGCTCGACA GGACGCTACC TATGCCAGCC AGACTCCCCA 
GAGGACGCTG AGCCTCTCCT GCCTCTCCAG TCAGCCCTTT CTGCAGTTTC TTCCATGAGA 
GCCCAAACCC TTGAACGTGA AGTGCCTTCC TCCACAGACG GCCAGCGCCC TGCAGATAGC 
GCCATTGTTC ATTCCACATC CGACCCCATC ATGACCGCCA GAGGGATGAG GCCTCTTCAG 
AGCCGCCTCC CCAAACCAGC TTCCTCAGGA AAAGTCAGTT CCCAAAAGCA GAATGAAGCA 
GAGCCAAGGC CTCAGACATG CTCATCATTC GGATATGCTG AAGACCCAAT GGCAAGCCAG 
CCGCTTCCAG ACTGGGGGAG TGAAGTTGCT GCCACCGGGA CCCAGAAATT GAGACAACTT 
GAAGAAACAA AAGACGATCC CGAGAATAGA TTATCGAAAA TTTCCCTAGA GTCATTCAAT 
AAATTTAACA GCAATACTGT GATTTTATTA GAAAAAGAGA AGAACTCTCT GAACAAGGTT 
GAAGGACAGA AGGAAGAAAA AGAAAAAAAT GAAGAGACAT CTTTGAGTAG TTCAGATAGG 
CCTGGGGTAG ACAACTTGGA ATCTTTGAGT GATTCTTTAT ATGATAGCTT CTCTTCCTGT 
GCCAGTCAAG GTTCAAATGA TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859466.1
CDS	1152..6938
Translation	MDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_017003977.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003977.2

ORF Insert Sequence:

ATGGACTCCA ATAAATACAT TGAGCATCTG CTGACTCAGC TTGAGGAGCA ACACAGGAGT 
CTCTGGAGGG AGAAGTTGGC AGTGGCCCGA CTACAGCGAG AAGTTGCCCA AAGAACAAGT 
GAGGGGGCCA TGCATGAGAA GCTGATACAT GAACTGGAAG AGGAGAGACA CTTACGTCTT 
CAAAGCGAGA AGCGGTTGCA GGAGGTGACC CTAGAGTCTG AACGCAACAG AATTCAGATG 
CGTAGCTTGC AGCAGCAGTT CTCCAGGATG GAAGAAACAG TACGAAATCT ATTGCAGAGT 
CAAGGATCTC CAGAGCAGAA AAAAGAAGAA ACTGTTAATA TAATGGTCTA TCAGGAAAAG 
CTGTCAGAGG AAGAGAGAAA ACATAAGGAA GCTTTGGAAG ATCTTCACAT GGTGGTTGAT 
GAGGATTCGA GGAGTGAAAG CAGCAGTACA GATGAGGGAA AAGAAAAGAC CAAATTGCTA 
TTAGAGAGAT TGAAAGCTCT AGAGGCAGAG AATTCAGCGT TGGCTTTGGA GAATGAAAAT 
CAAAGGGAAC AATATGAGCG ATGTCTTGAT GAGGTAGCCA ACCAAGTTGT TCAAGCTCTG 
CTTACTCAAA AGGATCTAAG AGAGGAATGT GTGAAGTTGA AAACAAGAGT GTTTGATTTG 
GAACAGCAGA ATCGAACACT AAGCATCCTA TTCCAACAGC GAGTCAGACC CACTTCTGAT 
CTGCTCCTCC AGAAACTTCA CTCACGTCTC TTGGATCTTT CATCTGGAGA TTTGCTTTCA 
GAGGTGGAAA GAAACCGAAG TCTGACACAG TCACGAACTG ATGCTGAGGT GCACGAACAC 
CAACTAAATA CAAAATCGGC CTTGAAATGC CCTGGCTTGG GGGCTGTCAT CCCTGGTCAT 
CTCTGTCCTC GAAACAGCTA CAGTAGCAGC AGTGAACTGT CTCTTTCAAG CACCTGCAGC 
GAGTACTCCA GTGGCTCCTC CTACACGTGG CACGATGGGA AGAACCTCAG GAAAAGGCAA 
TCATCACAAA ACTGGGATAA AAGGCTAAGT ATTGATTCTT CGCTCCCAAG TGGCTTTGCT 
AGTCCTACAA ATGAACTACC TCCAACTCGT ATCAAGGAAA GCCACATTTT GGAAGGGCTA 
AGAAAGCTAC AGAAGCGAAA AGTGTTACTT GAACCCCCAT CAGTGATAAC CAAATGGGGT 
TATAAAGATT GCATGAACTC GAATGAAGGA ATATATTCTC CTGGAATTAA AAGTAGCAGC 
CTCAAGGAGT ATCCCCCCTG CAAAACAGCT GACCTGGGGA GCCCCTGCAA GGAACCCCAC 
AAGACATTTG TTTATGATCT AGATTCCCAC GTTGATGCGG ACGATGACCC TTCCACCTTA 
GCATTGCTCC AAGCAGTTCC AAACCAGAGC TGCAGGCCAC ATGGCAGTAA ATTAACCCAC 
AGTGTTTCCG ACAGTCTGTT TGGCTGGGAG ACAAACAGAA AACACTTTCT GGAAGGCACA 
TCCTCAGTTT ATCCCAAGGA AAGGCCTGAA AAGCTGACAA GTTGCGCCAG CAGCTGTCCC 
TTAGAGATGA AGCTGTGCCC AAGTGTGCAG ACGCCTCAGG TACAGAGGGA GAGGGGCCCA 
CAGGGCCAAG GCCATGGCCG CATGGCTCTC AACCTCCAGC TTTCAGACAC TGATGACAAT 
GAAACGTTTG ATGAGCTGCA CATAGAGAGC AGTGATGAGA AAAGTCCTTC AGACGTGTCA 
TTGGCTGCCG ACACCGATAA GTCCGTGGAG AACCTGGATG TCCTTGTGGG GTTTGGAAAA 
TCTCTATGTG GGTCTCCTGA AGAGGAGGAA AAACAAGTGC CCATCCCTTC AGAGACTAGG 
CCAAAGACTT TTAGTTTCAT TAAGCAGCAA AGAGTTGTAA AAAGGACTTC TTCAGAAGAA 
TGTGTGACTG TGATATTTGA TGCGGAAGAC GGTGAGCCCA TTGAATTCAG CTCTCACCAG 
ACTGGGGTTG TCACTGTTAC CAGAAATGAG ATTTCCATCA ATTCAACTCC TGCTGGACCC 
AAGGCAGAAC ATACTGAGCT TTTACCTCAG GGAATTGCTT GTTTACAGCC AAGAGCTGCT 
GCAAGAGACT ATACTTTCTT TAAAAGGTCT GAAGAGGACA CTGAGAAAAA CATTCCAAAA 
GATAATGTAG ATAATGTTCC CAGGGTGTCC ACTGAATCTT TCAGCTCCAG GACAGTGACA 
CAAAATCCTC AGCAGCAAAA GCTGGTCAAA CCAACACACA ATATATCATG CCAGAGTAAT 
TCCAGGTCTT CGGCACCCAT GGGCATCTAT CAAAAGCAAA ATCTGACAAA AATACCTCCC 
AGGGGCAAGT CTTCACCTCA GAAATCAAAA CTAATGGAGC CCGAAGCCAC CACACTACTC 
CCTTCATCTG GCCTGGTGAC TCTTGAAAAA TCACCAGCCT TAGCTCCTGG GAAACTCTCA 
CGATTCATGA AGACTGAGAG CTCAGGGCCC CTCTTTGAAT TACGATCAGA TCCACACATT 
CCAAAACATT CCGCCCAACT TCCGCACAGC TCCAGGATGC CCAGCAGGAG GGACTGGGTC 
CAGTGCCCCA AGAGTCAGAC TCCAGGGTCA CGGTCAAGGC CTGCCATTGA GTCTAGTGAC 
AGTGGAGAGC CCCCCACGAG GGATGAACAC TGTGGCTCTG GGCCGGAGGC AGGGGTGAAA 
TCCCCTTCCC CTCCGCCCCC TCCAGGCAGG TCCGTCTCCC TGCTGGCCAG GCCCAGCTAT 
GACTATTCAC CAGCACCTTC ATCCACCAAG TCCGAAACCA GGGTCCCCAG TGAAACAGCA 
AGGACCCCAT TCAAATCCCC GCTGCTGAAA GGAATTTCTG CTCCAGTTAT TTCTTCTAAT 
CCGGCCACGA CAGAAGTGCA GAGGAAGAAA CCTTCTGTGG CCTTCAAAAA GCCTATCTTC 
ACTCACCCTA TGCCCTCCCC AGAAGCAGTC ATTCAAACCC GATGCCCTGC TCATGCCCCC 
TCCAGCTCCT TCACCGTAAT GGCTCTGGGG CCTCCAAAGG TCTCTCCGAA GAGAGGTGTC 
CCCAAAACCT CTCCTCGCCA AACACTTGGG ACCCCACAAA GGGACATAGG ATTACAGACT 
CCCAGGATCT CTCCTTCAAC CCATGAGCCA CTGGAAATGA CGTCCTCCAA AAGTGTATCT 
CCAGGGAGAA AAGGACAATT GAATGATAGC GCCTCCACAC CCCCCAAGCC TTCCTTCTTA 
GGGGTAAATG AGTCACCATC ATCTCAGGTC AGCAGTTCCT CATCATCCTC ATCACCCGCC 
AAAAGCCATA ACAGCCCTCA TGGTTGTCAA AGTGCTCATG AGAAAGGACT GAAAACTCGC 
CTTCCAGTGG GACTCAAAGT GCTCATGAAG TCTCCCCAGC TGCTCAGGAA AAGTTCCACC 
GTGCCAGGGA AACATGAAAA AGACAGTTTA AATGAAGCCT CCAAAAGTTC CGTGGCTGTG 
AACAAGTCTA AGCCAGAGGA CTCCAAGAAT CCAGCAAGCA TGGAGATCAC AGCGGGTGAA 
AGAAATGTGA CCCTACCGGA TTCACAAGCA CAGGGCAGTT TAGCTGATGG GCTTCCCCTG 
GAAACAGCAC TACAAGAGCC ATTGGAAAGT AGCATCCCTG GGAGTGATGG AAGGGATGGG 
GTAGATAATA GATCCATGAG AAGATCCCTT TCCTCCAGCA AACCACACCT AAAACCAGCT 
CTGGGTATGA ATGGCGCCAA AGCCCGCAGC CACAGCTTCA GTACACACTC AGGAGACAAG 
CCTTCTACGC CCCCCATCGA AGGGTCAGGC AAAGTCCGCA CTCAGATCAT TACCAATACC 
GCCGAGAGAG GCAATTCTCT TACCCGGCAG AACTCTTCCA CGGAAAGCTC TCCCAACAAG 
GCCCCTTCTG CTCCCATGTT GGAGAGTCTC CCCAGTGTTG GGAGGCCCTC GGGGCACCCC 
TCCTCCGGGA AGGGCTCCCT GGGGAGCTCA GGCAGCTTCA GCAGTCAGCA TGGGAGCCCA 
AGTAAGTTGC CTTTGAGGAT CCCTCCAAAG TCTGAGGGAC TCCTCATCCC ACCTGGAAAG 
GAAGACCAGC AGGCCTTCAC CCAGGGAGAG TGCCCCAGTG CCAATGTGGC TGTACTTGGG 
GAACCAGGCA GTGACCGCCG CAGTTGCCCA CCCACCCCAA CAGACTGCCC TGAAGCCCTG 
CAGAGCCCAG GGAGGACTCA GCATCCAAGC ACTTTTGAAA CAAGCAGTAC ATCCAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCCTCT GCAACCGCGA CTGATGCTGT GAGTTCAGAA 
GCCCCCCTCT CACCCACAAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA 
AAGGGCCAAG TGCAAACAAA GCCCACCTCT GTGGAAGCAA AGCAGAAGCC TGGGCCTTCT 
TTTGCCAGCT GGTTTGGTTT TCGGAAGAGT AGACTTCCAG CTCTGAGTAG CAGGAAAATG 
GACATCTCCA AAACCAAAGT AGAAAAGAAA GATGCAAAAG TCTTGGGGTT TGGAAACAGA 
CAACTAAAAT CAGAAAGAAA AAAAGAGAAA AAGAAGCCTG AACTACAGTG TGAGACAGAA 
AATGAGCTTA TCAAGGACAC CAAGTCAGCA GATAATCCAG ATGGCGGTTT ACAAAGCAAA 
AATAATCGGA GAACACCACA AGACATTTAC AACCAACTGA AGATTGAACC AAGGAATAGA 
CACAGCCCTG TTGCATGTTC AACGAAAGAC ACCTTCATGA CGGAACTCTT GAACAGAGTT 
GATAAGAAAG CAGCTCCACA GACAGAAAGT GGATCAAGTA ATGCTTCCTG CAGGAATGTG 
TTAAAGGGCA GTTCTCAGGG CTCCTGTCTC ATCGGCAGCT CTATCAGTAC TCAAGGAAAC 
CACAAGAAAA ACATGAAAAT CAAAGCCGAT ATGGAAGTAC CAAAAGACTC CCTGGTAAAA 
GAGGCAAATG AAAACTTGCA AGAGGATGAA GACGATGCAG TTGCAGATTC TGTATTTCAG 
AGCCACATCA TAGAATCCAA CTGCCAGATG AGAACATTGG ACAGTGGGAT CGGAACCTTT 
CCACTCCCAG ACTCGGGAAA TCGCTCGACA GGACGCTACC TATGCCAGCC AGACTCCCCA 
GAGGACGCTG AGCCTCTCCT GCCTCTCCAG TCAGCCCTTT CTGCAGTTTC TTCCATGAGA 
GCCCAAACCC TTGAACGTGA AGTGCCTTCC TCCACAGACG GCCAGCGCCC TGCAGATAGC 
GCCATTGTTC ATTCCACATC CGACCCCATC ATGACCGCCA GAGGGATGAG GCCTCTTCAG 
AGCCGCCTCC CCAAACCAGC TTCCTCAGGA AAAGTCAGTT CCCAAAAGCA GAATGAAGCA 
GAGCCAAGGC CTCAGACATG CTCATCATTC GGATATGCTG AAGACCCAAT GGCAAGCCAG 
CCGCTTCCAG ACTGGGGGAG TGAAGTTGCT GCCACCGGGA CCCAGAAATT GAGACAACTT 
GAAGAAACAA AAGACGATCC CGAGAATAGA TTATCGAAAA TTTCCCTAGA GTCATTCAAT 
AAATTTAACA GCAATACTGT GATTTTATTA GAAAAAGAGA AGAACTCTCT GAACAAGGTT 
GAAGGACAGA AGGAAGAAAA AGAAAAAAAT GAAGAGACAT CTTTGAGTAG TTCAGATAGG 
CCTGGGGTAG ACAACTTGGA ATCTTTGAGT GATTCTTTAT ATGATAGCTT CTCTTCCTGT 
GCCAGTCAAG GTTCAAATGA TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_017003976.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859465.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X9, mRNA.

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CloneID	OHu79436
Clone ID Related Accession (Same CDS sequence)	XM_017003976.2 , XM_011511102.2 , XM_017003977.1 , XM_017003977.2 , XM_017003976.1 , XM_011511101.1
Accession Version	XM_017003976.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA ATAAATACAT TGAGCATCTG CTGACTCAGC TTGAGGAGCA ACACAGGAGT 
CTCTGGAGGG AGAAGTTGGC AGTGGCCCGA CTACAGCGAG AAGTTGCCCA AAGAACAAGT 
GAGGGGGCCA TGCATGAGAA GCTGATACAT GAACTGGAAG AGGAGAGACA CTTACGTCTT 
CAAAGCGAGA AGCGGTTGCA GGAGGTGACC CTAGAGTCTG AACGCAACAG AATTCAGATG 
CGTAGCTTGC AGCAGCAGTT CTCCAGGATG GAAGAAACAG TACGAAATCT ATTGCAGAGT 
CAAGGATCTC CAGAGCAGAA AAAAGAAGAA ACTGTTAATA TAATGGTCTA TCAGGAAAAG 
CTGTCAGAGG AAGAGAGAAA ACATAAGGAA GCTTTGGAAG ATCTTCACAT GGTGGTTGAT 
GAGGATTCGA GGAGTGAAAG CAGCAGTACA GATGAGGGAA AAGAAAAGAC CAAATTGCTA 
TTAGAGAGAT TGAAAGCTCT AGAGGCAGAG AATTCAGCGT TGGCTTTGGA GAATGAAAAT 
CAAAGGGAAC AATATGAGCG ATGTCTTGAT GAGGTAGCCA ACCAAGTTGT TCAAGCTCTG 
CTTACTCAAA AGGATCTAAG AGAGGAATGT GTGAAGTTGA AAACAAGAGT GTTTGATTTG 
GAACAGCAGA ATCGAACACT AAGCATCCTA TTCCAACAGC GAGTCAGACC CACTTCTGAT 
CTGCTCCTCC AGAAACTTCA CTCACGTCTC TTGGATCTTT CATCTGGAGA TTTGCTTTCA 
GAGGTGGAAA GAAACCGAAG TCTGACACAG TCACGAACTG ATGCTGAGGT GCACGAACAC 
CAACTAAATA CAAAATCGGC CTTGAAATGC CCTGGCTTGG GGGCTGTCAT CCCTGGTCAT 
CTCTGTCCTC GAAACAGCTA CAGTAGCAGC AGTGAACTGT CTCTTTCAAG CACCTGCAGC 
GAGTACTCCA GTGGCTCCTC CTACACGTGG CACGATGGGA AGAACCTCAG GAAAAGGCAA 
TCATCACAAA ACTGGGATAA AAGGCTAAGT ATTGATTCTT CGCTCCCAAG TGGCTTTGCT 
AGTCCTACAA ATGAACTACC TCCAACTCGT ATCAAGGAAA GCCACATTTT GGAAGGGCTA 
AGAAAGCTAC AGAAGCGAAA AGTGTTACTT GAACCCCCAT CAGTGATAAC CAAATGGGGT 
TATAAAGATT GCATGAACTC GAATGAAGGA ATATATTCTC CTGGAATTAA AAGTAGCAGC 
CTCAAGGAGT ATCCCCCCTG CAAAACAGCT GACCTGGGGA GCCCCTGCAA GGAACCCCAC 
AAGACATTTG TTTATGATCT AGATTCCCAC GTTGATGCGG ACGATGACCC TTCCACCTTA 
GCATTGCTCC AAGCAGTTCC AAACCAGAGC TGCAGGCCAC ATGGCAGTAA ATTAACCCAC 
AGTGTTTCCG ACAGTCTGTT TGGCTGGGAG ACAAACAGAA AACACTTTCT GGAAGGCACA 
TCCTCAGTTT ATCCCAAGGA AAGGCCTGAA AAGCTGACAA GTTGCGCCAG CAGCTGTCCC 
TTAGAGATGA AGCTGTGCCC AAGTGTGCAG ACGCCTCAGG TACAGAGGGA GAGGGGCCCA 
CAGGGCCAAG GCCATGGCCG CATGGCTCTC AACCTCCAGC TTTCAGACAC TGATGACAAT 
GAAACGTTTG ATGAGCTGCA CATAGAGAGC AGTGATGAGA AAAGTCCTTC AGACGTGTCA 
TTGGCTGCCG ACACCGATAA GTCCGTGGAG AACCTGGATG TCCTTGTGGG GTTTGGAAAA 
TCTCTATGTG GGTCTCCTGA AGAGGAGGAA AAACAAGTGC CCATCCCTTC AGAGACTAGG 
CCAAAGACTT TTAGTTTCAT TAAGCAGCAA AGAGTTGTAA AAAGGACTTC TTCAGAAGAA 
TGTGTGACTG TGATATTTGA TGCGGAAGAC GGTGAGCCCA TTGAATTCAG CTCTCACCAG 
ACTGGGGTTG TCACTGTTAC CAGAAATGAG ATTTCCATCA ATTCAACTCC TGCTGGACCC 
AAGGCAGAAC ATACTGAGCT TTTACCTCAG GGAATTGCTT GTTTACAGCC AAGAGCTGCT 
GCAAGAGACT ATACTTTCTT TAAAAGGTCT GAAGAGGACA CTGAGAAAAA CATTCCAAAA 
GATAATGTAG ATAATGTTCC CAGGGTGTCC ACTGAATCTT TCAGCTCCAG GACAGTGACA 
CAAAATCCTC AGCAGCAAAA GCTGGTCAAA CCAACACACA ATATATCATG CCAGAGTAAT 
TCCAGGTCTT CGGCACCCAT GGGCATCTAT CAAAAGCAAA ATCTGACAAA AATACCTCCC 
AGGGGCAAGT CTTCACCTCA GAAATCAAAA CTAATGGAGC CCGAAGCCAC CACACTACTC 
CCTTCATCTG GCCTGGTGAC TCTTGAAAAA TCACCAGCCT TAGCTCCTGG GAAACTCTCA 
CGATTCATGA AGACTGAGAG CTCAGGGCCC CTCTTTGAAT TACGATCAGA TCCACACATT 
CCAAAACATT CCGCCCAACT TCCGCACAGC TCCAGGATGC CCAGCAGGAG GGACTGGGTC 
CAGTGCCCCA AGAGTCAGAC TCCAGGGTCA CGGTCAAGGC CTGCCATTGA GTCTAGTGAC 
AGTGGAGAGC CCCCCACGAG GGATGAACAC TGTGGCTCTG GGCCGGAGGC AGGGGTGAAA 
TCCCCTTCCC CTCCGCCCCC TCCAGGCAGG TCCGTCTCCC TGCTGGCCAG GCCCAGCTAT 
GACTATTCAC CAGCACCTTC ATCCACCAAG TCCGAAACCA GGGTCCCCAG TGAAACAGCA 
AGGACCCCAT TCAAATCCCC GCTGCTGAAA GGAATTTCTG CTCCAGTTAT TTCTTCTAAT 
CCGGCCACGA CAGAAGTGCA GAGGAAGAAA CCTTCTGTGG CCTTCAAAAA GCCTATCTTC 
ACTCACCCTA TGCCCTCCCC AGAAGCAGTC ATTCAAACCC GATGCCCTGC TCATGCCCCC 
TCCAGCTCCT TCACCGTAAT GGCTCTGGGG CCTCCAAAGG TCTCTCCGAA GAGAGGTGTC 
CCCAAAACCT CTCCTCGCCA AACACTTGGG ACCCCACAAA GGGACATAGG ATTACAGACT 
CCCAGGATCT CTCCTTCAAC CCATGAGCCA CTGGAAATGA CGTCCTCCAA AAGTGTATCT 
CCAGGGAGAA AAGGACAATT GAATGATAGC GCCTCCACAC CCCCCAAGCC TTCCTTCTTA 
GGGGTAAATG AGTCACCATC ATCTCAGGTC AGCAGTTCCT CATCATCCTC ATCACCCGCC 
AAAAGCCATA ACAGCCCTCA TGGTTGTCAA AGTGCTCATG AGAAAGGACT GAAAACTCGC 
CTTCCAGTGG GACTCAAAGT GCTCATGAAG TCTCCCCAGC TGCTCAGGAA AAGTTCCACC 
GTGCCAGGGA AACATGAAAA AGACAGTTTA AATGAAGCCT CCAAAAGTTC CGTGGCTGTG 
AACAAGTCTA AGCCAGAGGA CTCCAAGAAT CCAGCAAGCA TGGAGATCAC AGCGGGTGAA 
AGAAATGTGA CCCTACCGGA TTCACAAGCA CAGGGCAGTT TAGCTGATGG GCTTCCCCTG 
GAAACAGCAC TACAAGAGCC ATTGGAAAGT AGCATCCCTG GGAGTGATGG AAGGGATGGG 
GTAGATAATA GATCCATGAG AAGATCCCTT TCCTCCAGCA AACCACACCT AAAACCAGCT 
CTGGGTATGA ATGGCGCCAA AGCCCGCAGC CACAGCTTCA GTACACACTC AGGAGACAAG 
CCTTCTACGC CCCCCATCGA AGGGTCAGGC AAAGTCCGCA CTCAGATCAT TACCAATACC 
GCCGAGAGAG GCAATTCTCT TACCCGGCAG AACTCTTCCA CGGAAAGCTC TCCCAACAAG 
GCCCCTTCTG CTCCCATGTT GGAGAGTCTC CCCAGTGTTG GGAGGCCCTC GGGGCACCCC 
TCCTCCGGGA AGGGCTCCCT GGGGAGCTCA GGCAGCTTCA GCAGTCAGCA TGGGAGCCCA 
AGTAAGTTGC CTTTGAGGAT CCCTCCAAAG TCTGAGGGAC TCCTCATCCC ACCTGGAAAG 
GAAGACCAGC AGGCCTTCAC CCAGGGAGAG TGCCCCAGTG CCAATGTGGC TGTACTTGGG 
GAACCAGGCA GTGACCGCCG CAGTTGCCCA CCCACCCCAA CAGACTGCCC TGAAGCCCTG 
CAGAGCCCAG GGAGGACTCA GCATCCAAGC ACTTTTGAAA CAAGCAGTAC ATCCAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCCTCT GCAACCGCGA CTGATGCTGT GAGTTCAGAA 
GCCCCCCTCT CACCCACAAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA 
AAGGGCCAAG TGCAAACAAA GCCCACCTCT GTGGAAGCAA AGCAGAAGCC TGGGCCTTCT 
TTTGCCAGCT GGTTTGGTTT TCGGAAGAGT AGACTTCCAG CTCTGAGTAG CAGGAAAATG 
GACATCTCCA AAACCAAAGT AGAAAAGAAA GATGCAAAAG TCTTGGGGTT TGGAAACAGA 
CAACTAAAAT CAGAAAGAAA AAAAGAGAAA AAGAAGCCTG AACTACAGTG TGAGACAGAA 
AATGAGCTTA TCAAGGACAC CAAGTCAGCA GATAATCCAG ATGGCGGTTT ACAAAGCAAA 
AATAATCGGA GAACACCACA AGACATTTAC AACCAACTGA AGATTGAACC AAGGAATAGA 
CACAGCCCTG TTGCATGTTC AACGAAAGAC ACCTTCATGA CGGAACTCTT GAACAGAGTT 
GATAAGAAAG CAGCTCCACA GACAGAAAGT GGATCAAGTA ATGCTTCCTG CAGGAATGTG 
TTAAAGGGCA GTTCTCAGGG CTCCTGTCTC ATCGGCAGCT CTATCAGTAC TCAAGGAAAC 
CACAAGAAAA ACATGAAAAT CAAAGCCGAT ATGGAAGTAC CAAAAGACTC CCTGGTAAAA 
GAGGCAAATG AAAACTTGCA AGAGGATGAA GACGATGCAG TTGCAGATTC TGTATTTCAG 
AGCCACATCA TAGAATCCAA CTGCCAGATG AGAACATTGG ACAGTGGGAT CGGAACCTTT 
CCACTCCCAG ACTCGGGAAA TCGCTCGACA GGACGCTACC TATGCCAGCC AGACTCCCCA 
GAGGACGCTG AGCCTCTCCT GCCTCTCCAG TCAGCCCTTT CTGCAGTTTC TTCCATGAGA 
GCCCAAACCC TTGAACGTGA AGTGCCTTCC TCCACAGACG GCCAGCGCCC TGCAGATAGC 
GCCATTGTTC ATTCCACATC CGACCCCATC ATGACCGCCA GAGGGATGAG GCCTCTTCAG 
AGCCGCCTCC CCAAACCAGC TTCCTCAGGA AAAGTCAGTT CCCAAAAGCA GAATGAAGCA 
GAGCCAAGGC CTCAGACATG CTCATCATTC GGATATGCTG AAGACCCAAT GGCAAGCCAG 
CCGCTTCCAG ACTGGGGGAG TGAAGTTGCT GCCACCGGGA CCCAGAAATT GAGACAACTT 
GAAGAAACAA AAGACGATCC CGAGAATAGA TTATCGAAAA TTTCCCTAGA GTCATTCAAT 
AAATTTAACA GCAATACTGT GATTTTATTA GAAAAAGAGA AGAACTCTCT GAACAAGGTT 
GAAGGACAGA AGGAAGAAAA AGAAAAAAAT GAAGAGACAT CTTTGAGTAG TTCAGATAGG 
CCTGGGGTAG ACAACTTGGA ATCTTTGAGT GATTCTTTAT ATGATAGCTT CTCTTCCTGT 
GCCAGTCAAG GTTCAAATGA TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859465.1
CDS	1241..7027
Translation	MDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_017003976.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003976.1

ORF Insert Sequence:

ATGGACTCCA ATAAATACAT TGAGCATCTG CTGACTCAGC TTGAGGAGCA ACACAGGAGT 
CTCTGGAGGG AGAAGTTGGC AGTGGCCCGA CTACAGCGAG AAGTTGCCCA AAGAACAAGT 
GAGGGGGCCA TGCATGAGAA GCTGATACAT GAACTGGAAG AGGAGAGACA CTTACGTCTT 
CAAAGCGAGA AGCGGTTGCA GGAGGTGACC CTAGAGTCTG AACGCAACAG AATTCAGATG 
CGTAGCTTGC AGCAGCAGTT CTCCAGGATG GAAGAAACAG TACGAAATCT ATTGCAGAGT 
CAAGGATCTC CAGAGCAGAA AAAAGAAGAA ACTGTTAATA TAATGGTCTA TCAGGAAAAG 
CTGTCAGAGG AAGAGAGAAA ACATAAGGAA GCTTTGGAAG ATCTTCACAT GGTGGTTGAT 
GAGGATTCGA GGAGTGAAAG CAGCAGTACA GATGAGGGAA AAGAAAAGAC CAAATTGCTA 
TTAGAGAGAT TGAAAGCTCT AGAGGCAGAG AATTCAGCGT TGGCTTTGGA GAATGAAAAT 
CAAAGGGAAC AATATGAGCG ATGTCTTGAT GAGGTAGCCA ACCAAGTTGT TCAAGCTCTG 
CTTACTCAAA AGGATCTAAG AGAGGAATGT GTGAAGTTGA AAACAAGAGT GTTTGATTTG 
GAACAGCAGA ATCGAACACT AAGCATCCTA TTCCAACAGC GAGTCAGACC CACTTCTGAT 
CTGCTCCTCC AGAAACTTCA CTCACGTCTC TTGGATCTTT CATCTGGAGA TTTGCTTTCA 
GAGGTGGAAA GAAACCGAAG TCTGACACAG TCACGAACTG ATGCTGAGGT GCACGAACAC 
CAACTAAATA CAAAATCGGC CTTGAAATGC CCTGGCTTGG GGGCTGTCAT CCCTGGTCAT 
CTCTGTCCTC GAAACAGCTA CAGTAGCAGC AGTGAACTGT CTCTTTCAAG CACCTGCAGC 
GAGTACTCCA GTGGCTCCTC CTACACGTGG CACGATGGGA AGAACCTCAG GAAAAGGCAA 
TCATCACAAA ACTGGGATAA AAGGCTAAGT ATTGATTCTT CGCTCCCAAG TGGCTTTGCT 
AGTCCTACAA ATGAACTACC TCCAACTCGT ATCAAGGAAA GCCACATTTT GGAAGGGCTA 
AGAAAGCTAC AGAAGCGAAA AGTGTTACTT GAACCCCCAT CAGTGATAAC CAAATGGGGT 
TATAAAGATT GCATGAACTC GAATGAAGGA ATATATTCTC CTGGAATTAA AAGTAGCAGC 
CTCAAGGAGT ATCCCCCCTG CAAAACAGCT GACCTGGGGA GCCCCTGCAA GGAACCCCAC 
AAGACATTTG TTTATGATCT AGATTCCCAC GTTGATGCGG ACGATGACCC TTCCACCTTA 
GCATTGCTCC AAGCAGTTCC AAACCAGAGC TGCAGGCCAC ATGGCAGTAA ATTAACCCAC 
AGTGTTTCCG ACAGTCTGTT TGGCTGGGAG ACAAACAGAA AACACTTTCT GGAAGGCACA 
TCCTCAGTTT ATCCCAAGGA AAGGCCTGAA AAGCTGACAA GTTGCGCCAG CAGCTGTCCC 
TTAGAGATGA AGCTGTGCCC AAGTGTGCAG ACGCCTCAGG TACAGAGGGA GAGGGGCCCA 
CAGGGCCAAG GCCATGGCCG CATGGCTCTC AACCTCCAGC TTTCAGACAC TGATGACAAT 
GAAACGTTTG ATGAGCTGCA CATAGAGAGC AGTGATGAGA AAAGTCCTTC AGACGTGTCA 
TTGGCTGCCG ACACCGATAA GTCCGTGGAG AACCTGGATG TCCTTGTGGG GTTTGGAAAA 
TCTCTATGTG GGTCTCCTGA AGAGGAGGAA AAACAAGTGC CCATCCCTTC AGAGACTAGG 
CCAAAGACTT TTAGTTTCAT TAAGCAGCAA AGAGTTGTAA AAAGGACTTC TTCAGAAGAA 
TGTGTGACTG TGATATTTGA TGCGGAAGAC GGTGAGCCCA TTGAATTCAG CTCTCACCAG 
ACTGGGGTTG TCACTGTTAC CAGAAATGAG ATTTCCATCA ATTCAACTCC TGCTGGACCC 
AAGGCAGAAC ATACTGAGCT TTTACCTCAG GGAATTGCTT GTTTACAGCC AAGAGCTGCT 
GCAAGAGACT ATACTTTCTT TAAAAGGTCT GAAGAGGACA CTGAGAAAAA CATTCCAAAA 
GATAATGTAG ATAATGTTCC CAGGGTGTCC ACTGAATCTT TCAGCTCCAG GACAGTGACA 
CAAAATCCTC AGCAGCAAAA GCTGGTCAAA CCAACACACA ATATATCATG CCAGAGTAAT 
TCCAGGTCTT CGGCACCCAT GGGCATCTAT CAAAAGCAAA ATCTGACAAA AATACCTCCC 
AGGGGCAAGT CTTCACCTCA GAAATCAAAA CTAATGGAGC CCGAAGCCAC CACACTACTC 
CCTTCATCTG GCCTGGTGAC TCTTGAAAAA TCACCAGCCT TAGCTCCTGG GAAACTCTCA 
CGATTCATGA AGACTGAGAG CTCAGGGCCC CTCTTTGAAT TACGATCAGA TCCACACATT 
CCAAAACATT CCGCCCAACT TCCGCACAGC TCCAGGATGC CCAGCAGGAG GGACTGGGTC 
CAGTGCCCCA AGAGTCAGAC TCCAGGGTCA CGGTCAAGGC CTGCCATTGA GTCTAGTGAC 
AGTGGAGAGC CCCCCACGAG GGATGAACAC TGTGGCTCTG GGCCGGAGGC AGGGGTGAAA 
TCCCCTTCCC CTCCGCCCCC TCCAGGCAGG TCCGTCTCCC TGCTGGCCAG GCCCAGCTAT 
GACTATTCAC CAGCACCTTC ATCCACCAAG TCCGAAACCA GGGTCCCCAG TGAAACAGCA 
AGGACCCCAT TCAAATCCCC GCTGCTGAAA GGAATTTCTG CTCCAGTTAT TTCTTCTAAT 
CCGGCCACGA CAGAAGTGCA GAGGAAGAAA CCTTCTGTGG CCTTCAAAAA GCCTATCTTC 
ACTCACCCTA TGCCCTCCCC AGAAGCAGTC ATTCAAACCC GATGCCCTGC TCATGCCCCC 
TCCAGCTCCT TCACCGTAAT GGCTCTGGGG CCTCCAAAGG TCTCTCCGAA GAGAGGTGTC 
CCCAAAACCT CTCCTCGCCA AACACTTGGG ACCCCACAAA GGGACATAGG ATTACAGACT 
CCCAGGATCT CTCCTTCAAC CCATGAGCCA CTGGAAATGA CGTCCTCCAA AAGTGTATCT 
CCAGGGAGAA AAGGACAATT GAATGATAGC GCCTCCACAC CCCCCAAGCC TTCCTTCTTA 
GGGGTAAATG AGTCACCATC ATCTCAGGTC AGCAGTTCCT CATCATCCTC ATCACCCGCC 
AAAAGCCATA ACAGCCCTCA TGGTTGTCAA AGTGCTCATG AGAAAGGACT GAAAACTCGC 
CTTCCAGTGG GACTCAAAGT GCTCATGAAG TCTCCCCAGC TGCTCAGGAA AAGTTCCACC 
GTGCCAGGGA AACATGAAAA AGACAGTTTA AATGAAGCCT CCAAAAGTTC CGTGGCTGTG 
AACAAGTCTA AGCCAGAGGA CTCCAAGAAT CCAGCAAGCA TGGAGATCAC AGCGGGTGAA 
AGAAATGTGA CCCTACCGGA TTCACAAGCA CAGGGCAGTT TAGCTGATGG GCTTCCCCTG 
GAAACAGCAC TACAAGAGCC ATTGGAAAGT AGCATCCCTG GGAGTGATGG AAGGGATGGG 
GTAGATAATA GATCCATGAG AAGATCCCTT TCCTCCAGCA AACCACACCT AAAACCAGCT 
CTGGGTATGA ATGGCGCCAA AGCCCGCAGC CACAGCTTCA GTACACACTC AGGAGACAAG 
CCTTCTACGC CCCCCATCGA AGGGTCAGGC AAAGTCCGCA CTCAGATCAT TACCAATACC 
GCCGAGAGAG GCAATTCTCT TACCCGGCAG AACTCTTCCA CGGAAAGCTC TCCCAACAAG 
GCCCCTTCTG CTCCCATGTT GGAGAGTCTC CCCAGTGTTG GGAGGCCCTC GGGGCACCCC 
TCCTCCGGGA AGGGCTCCCT GGGGAGCTCA GGCAGCTTCA GCAGTCAGCA TGGGAGCCCA 
AGTAAGTTGC CTTTGAGGAT CCCTCCAAAG TCTGAGGGAC TCCTCATCCC ACCTGGAAAG 
GAAGACCAGC AGGCCTTCAC CCAGGGAGAG TGCCCCAGTG CCAATGTGGC TGTACTTGGG 
GAACCAGGCA GTGACCGCCG CAGTTGCCCA CCCACCCCAA CAGACTGCCC TGAAGCCCTG 
CAGAGCCCAG GGAGGACTCA GCATCCAAGC ACTTTTGAAA CAAGCAGTAC ATCCAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCCTCT GCAACCGCGA CTGATGCTGT GAGTTCAGAA 
GCCCCCCTCT CACCCACAAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA 
AAGGGCCAAG TGCAAACAAA GCCCACCTCT GTGGAAGCAA AGCAGAAGCC TGGGCCTTCT 
TTTGCCAGCT GGTTTGGTTT TCGGAAGAGT AGACTTCCAG CTCTGAGTAG CAGGAAAATG 
GACATCTCCA AAACCAAAGT AGAAAAGAAA GATGCAAAAG TCTTGGGGTT TGGAAACAGA 
CAACTAAAAT CAGAAAGAAA AAAAGAGAAA AAGAAGCCTG AACTACAGTG TGAGACAGAA 
AATGAGCTTA TCAAGGACAC CAAGTCAGCA GATAATCCAG ATGGCGGTTT ACAAAGCAAA 
AATAATCGGA GAACACCACA AGACATTTAC AACCAACTGA AGATTGAACC AAGGAATAGA 
CACAGCCCTG TTGCATGTTC AACGAAAGAC ACCTTCATGA CGGAACTCTT GAACAGAGTT 
GATAAGAAAG CAGCTCCACA GACAGAAAGT GGATCAAGTA ATGCTTCCTG CAGGAATGTG 
TTAAAGGGCA GTTCTCAGGG CTCCTGTCTC ATCGGCAGCT CTATCAGTAC TCAAGGAAAC 
CACAAGAAAA ACATGAAAAT CAAAGCCGAT ATGGAAGTAC CAAAAGACTC CCTGGTAAAA 
GAGGCAAATG AAAACTTGCA AGAGGATGAA GACGATGCAG TTGCAGATTC TGTATTTCAG 
AGCCACATCA TAGAATCCAA CTGCCAGATG AGAACATTGG ACAGTGGGAT CGGAACCTTT 
CCACTCCCAG ACTCGGGAAA TCGCTCGACA GGACGCTACC TATGCCAGCC AGACTCCCCA 
GAGGACGCTG AGCCTCTCCT GCCTCTCCAG TCAGCCCTTT CTGCAGTTTC TTCCATGAGA 
GCCCAAACCC TTGAACGTGA AGTGCCTTCC TCCACAGACG GCCAGCGCCC TGCAGATAGC 
GCCATTGTTC ATTCCACATC CGACCCCATC ATGACCGCCA GAGGGATGAG GCCTCTTCAG 
AGCCGCCTCC CCAAACCAGC TTCCTCAGGA AAAGTCAGTT CCCAAAAGCA GAATGAAGCA 
GAGCCAAGGC CTCAGACATG CTCATCATTC GGATATGCTG AAGACCCAAT GGCAAGCCAG 
CCGCTTCCAG ACTGGGGGAG TGAAGTTGCT GCCACCGGGA CCCAGAAATT GAGACAACTT 
GAAGAAACAA AAGACGATCC CGAGAATAGA TTATCGAAAA TTTCCCTAGA GTCATTCAAT 
AAATTTAACA GCAATACTGT GATTTTATTA GAAAAAGAGA AGAACTCTCT GAACAAGGTT 
GAAGGACAGA AGGAAGAAAA AGAAAAAAAT GAAGAGACAT CTTTGAGTAG TTCAGATAGG 
CCTGGGGTAG ACAACTTGGA ATCTTTGAGT GATTCTTTAT ATGATAGCTT CTCTTCCTGT 
GCCAGTCAAG GTTCAAATGA TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_011511101.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509403.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X9, mRNA.

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CloneID	OHu79436
Clone ID Related Accession (Same CDS sequence)	XM_017003976.2 , XM_011511102.2 , XM_017003977.1 , XM_017003977.2 , XM_017003976.1 , XM_011511101.1
Accession Version	XM_011511101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA ATAAATACAT TGAGCATCTG CTGACTCAGC TTGAGGAGCA ACACAGGAGT 
CTCTGGAGGG AGAAGTTGGC AGTGGCCCGA CTACAGCGAG AAGTTGCCCA AAGAACAAGT 
GAGGGGGCCA TGCATGAGAA GCTGATACAT GAACTGGAAG AGGAGAGACA CTTACGTCTT 
CAAAGCGAGA AGCGGTTGCA GGAGGTGACC CTAGAGTCTG AACGCAACAG AATTCAGATG 
CGTAGCTTGC AGCAGCAGTT CTCCAGGATG GAAGAAACAG TACGAAATCT ATTGCAGAGT 
CAAGGATCTC CAGAGCAGAA AAAAGAAGAA ACTGTTAATA TAATGGTCTA TCAGGAAAAG 
CTGTCAGAGG AAGAGAGAAA ACATAAGGAA GCTTTGGAAG ATCTTCACAT GGTGGTTGAT 
GAGGATTCGA GGAGTGAAAG CAGCAGTACA GATGAGGGAA AAGAAAAGAC CAAATTGCTA 
TTAGAGAGAT TGAAAGCTCT AGAGGCAGAG AATTCAGCGT TGGCTTTGGA GAATGAAAAT 
CAAAGGGAAC AATATGAGCG ATGTCTTGAT GAGGTAGCCA ACCAAGTTGT TCAAGCTCTG 
CTTACTCAAA AGGATCTAAG AGAGGAATGT GTGAAGTTGA AAACAAGAGT GTTTGATTTG 
GAACAGCAGA ATCGAACACT AAGCATCCTA TTCCAACAGC GAGTCAGACC CACTTCTGAT 
CTGCTCCTCC AGAAACTTCA CTCACGTCTC TTGGATCTTT CATCTGGAGA TTTGCTTTCA 
GAGGTGGAAA GAAACCGAAG TCTGACACAG TCACGAACTG ATGCTGAGGT GCACGAACAC 
CAACTAAATA CAAAATCGGC CTTGAAATGC CCTGGCTTGG GGGCTGTCAT CCCTGGTCAT 
CTCTGTCCTC GAAACAGCTA CAGTAGCAGC AGTGAACTGT CTCTTTCAAG CACCTGCAGC 
GAGTACTCCA GTGGCTCCTC CTACACGTGG CACGATGGGA AGAACCTCAG GAAAAGGCAA 
TCATCACAAA ACTGGGATAA AAGGCTAAGT ATTGATTCTT CGCTCCCAAG TGGCTTTGCT 
AGTCCTACAA ATGAACTACC TCCAACTCGT ATCAAGGAAA GCCACATTTT GGAAGGGCTA 
AGAAAGCTAC AGAAGCGAAA AGTGTTACTT GAACCCCCAT CAGTGATAAC CAAATGGGGT 
TATAAAGATT GCATGAACTC GAATGAAGGA ATATATTCTC CTGGAATTAA AAGTAGCAGC 
CTCAAGGAGT ATCCCCCCTG CAAAACAGCT GACCTGGGGA GCCCCTGCAA GGAACCCCAC 
AAGACATTTG TTTATGATCT AGATTCCCAC GTTGATGCGG ACGATGACCC TTCCACCTTA 
GCATTGCTCC AAGCAGTTCC AAACCAGAGC TGCAGGCCAC ATGGCAGTAA ATTAACCCAC 
AGTGTTTCCG ACAGTCTGTT TGGCTGGGAG ACAAACAGAA AACACTTTCT GGAAGGCACA 
TCCTCAGTTT ATCCCAAGGA AAGGCCTGAA AAGCTGACAA GTTGCGCCAG CAGCTGTCCC 
TTAGAGATGA AGCTGTGCCC AAGTGTGCAG ACGCCTCAGG TACAGAGGGA GAGGGGCCCA 
CAGGGCCAAG GCCATGGCCG CATGGCTCTC AACCTCCAGC TTTCAGACAC TGATGACAAT 
GAAACGTTTG ATGAGCTGCA CATAGAGAGC AGTGATGAGA AAAGTCCTTC AGACGTGTCA 
TTGGCTGCCG ACACCGATAA GTCCGTGGAG AACCTGGATG TCCTTGTGGG GTTTGGAAAA 
TCTCTATGTG GGTCTCCTGA AGAGGAGGAA AAACAAGTGC CCATCCCTTC AGAGACTAGG 
CCAAAGACTT TTAGTTTCAT TAAGCAGCAA AGAGTTGTAA AAAGGACTTC TTCAGAAGAA 
TGTGTGACTG TGATATTTGA TGCGGAAGAC GGTGAGCCCA TTGAATTCAG CTCTCACCAG 
ACTGGGGTTG TCACTGTTAC CAGAAATGAG ATTTCCATCA ATTCAACTCC TGCTGGACCC 
AAGGCAGAAC ATACTGAGCT TTTACCTCAG GGAATTGCTT GTTTACAGCC AAGAGCTGCT 
GCAAGAGACT ATACTTTCTT TAAAAGGTCT GAAGAGGACA CTGAGAAAAA CATTCCAAAA 
GATAATGTAG ATAATGTTCC CAGGGTGTCC ACTGAATCTT TCAGCTCCAG GACAGTGACA 
CAAAATCCTC AGCAGCAAAA GCTGGTCAAA CCAACACACA ATATATCATG CCAGAGTAAT 
TCCAGGTCTT CGGCACCCAT GGGCATCTAT CAAAAGCAAA ATCTGACAAA AATACCTCCC 
AGGGGCAAGT CTTCACCTCA GAAATCAAAA CTAATGGAGC CCGAAGCCAC CACACTACTC 
CCTTCATCTG GCCTGGTGAC TCTTGAAAAA TCACCAGCCT TAGCTCCTGG GAAACTCTCA 
CGATTCATGA AGACTGAGAG CTCAGGGCCC CTCTTTGAAT TACGATCAGA TCCACACATT 
CCAAAACATT CCGCCCAACT TCCGCACAGC TCCAGGATGC CCAGCAGGAG GGACTGGGTC 
CAGTGCCCCA AGAGTCAGAC TCCAGGGTCA CGGTCAAGGC CTGCCATTGA GTCTAGTGAC 
AGTGGAGAGC CCCCCACGAG GGATGAACAC TGTGGCTCTG GGCCGGAGGC AGGGGTGAAA 
TCCCCTTCCC CTCCGCCCCC TCCAGGCAGG TCCGTCTCCC TGCTGGCCAG GCCCAGCTAT 
GACTATTCAC CAGCACCTTC ATCCACCAAG TCCGAAACCA GGGTCCCCAG TGAAACAGCA 
AGGACCCCAT TCAAATCCCC GCTGCTGAAA GGAATTTCTG CTCCAGTTAT TTCTTCTAAT 
CCGGCCACGA CAGAAGTGCA GAGGAAGAAA CCTTCTGTGG CCTTCAAAAA GCCTATCTTC 
ACTCACCCTA TGCCCTCCCC AGAAGCAGTC ATTCAAACCC GATGCCCTGC TCATGCCCCC 
TCCAGCTCCT TCACCGTAAT GGCTCTGGGG CCTCCAAAGG TCTCTCCGAA GAGAGGTGTC 
CCCAAAACCT CTCCTCGCCA AACACTTGGG ACCCCACAAA GGGACATAGG ATTACAGACT 
CCCAGGATCT CTCCTTCAAC CCATGAGCCA CTGGAAATGA CGTCCTCCAA AAGTGTATCT 
CCAGGGAGAA AAGGACAATT GAATGATAGC GCCTCCACAC CCCCCAAGCC TTCCTTCTTA 
GGGGTAAATG AGTCACCATC ATCTCAGGTC AGCAGTTCCT CATCATCCTC ATCACCCGCC 
AAAAGCCATA ACAGCCCTCA TGGTTGTCAA AGTGCTCATG AGAAAGGACT GAAAACTCGC 
CTTCCAGTGG GACTCAAAGT GCTCATGAAG TCTCCCCAGC TGCTCAGGAA AAGTTCCACC 
GTGCCAGGGA AACATGAAAA AGACAGTTTA AATGAAGCCT CCAAAAGTTC CGTGGCTGTG 
AACAAGTCTA AGCCAGAGGA CTCCAAGAAT CCAGCAAGCA TGGAGATCAC AGCGGGTGAA 
AGAAATGTGA CCCTACCGGA TTCACAAGCA CAGGGCAGTT TAGCTGATGG GCTTCCCCTG 
GAAACAGCAC TACAAGAGCC ATTGGAAAGT AGCATCCCTG GGAGTGATGG AAGGGATGGG 
GTAGATAATA GATCCATGAG AAGATCCCTT TCCTCCAGCA AACCACACCT AAAACCAGCT 
CTGGGTATGA ATGGCGCCAA AGCCCGCAGC CACAGCTTCA GTACACACTC AGGAGACAAG 
CCTTCTACGC CCCCCATCGA AGGGTCAGGC AAAGTCCGCA CTCAGATCAT TACCAATACC 
GCCGAGAGAG GCAATTCTCT TACCCGGCAG AACTCTTCCA CGGAAAGCTC TCCCAACAAG 
GCCCCTTCTG CTCCCATGTT GGAGAGTCTC CCCAGTGTTG GGAGGCCCTC GGGGCACCCC 
TCCTCCGGGA AGGGCTCCCT GGGGAGCTCA GGCAGCTTCA GCAGTCAGCA TGGGAGCCCA 
AGTAAGTTGC CTTTGAGGAT CCCTCCAAAG TCTGAGGGAC TCCTCATCCC ACCTGGAAAG 
GAAGACCAGC AGGCCTTCAC CCAGGGAGAG TGCCCCAGTG CCAATGTGGC TGTACTTGGG 
GAACCAGGCA GTGACCGCCG CAGTTGCCCA CCCACCCCAA CAGACTGCCC TGAAGCCCTG 
CAGAGCCCAG GGAGGACTCA GCATCCAAGC ACTTTTGAAA CAAGCAGTAC ATCCAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCCTCT GCAACCGCGA CTGATGCTGT GAGTTCAGAA 
GCCCCCCTCT CACCCACAAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA 
AAGGGCCAAG TGCAAACAAA GCCCACCTCT GTGGAAGCAA AGCAGAAGCC TGGGCCTTCT 
TTTGCCAGCT GGTTTGGTTT TCGGAAGAGT AGACTTCCAG CTCTGAGTAG CAGGAAAATG 
GACATCTCCA AAACCAAAGT AGAAAAGAAA GATGCAAAAG TCTTGGGGTT TGGAAACAGA 
CAACTAAAAT CAGAAAGAAA AAAAGAGAAA AAGAAGCCTG AACTACAGTG TGAGACAGAA 
AATGAGCTTA TCAAGGACAC CAAGTCAGCA GATAATCCAG ATGGCGGTTT ACAAAGCAAA 
AATAATCGGA GAACACCACA AGACATTTAC AACCAACTGA AGATTGAACC AAGGAATAGA 
CACAGCCCTG TTGCATGTTC AACGAAAGAC ACCTTCATGA CGGAACTCTT GAACAGAGTT 
GATAAGAAAG CAGCTCCACA GACAGAAAGT GGATCAAGTA ATGCTTCCTG CAGGAATGTG 
TTAAAGGGCA GTTCTCAGGG CTCCTGTCTC ATCGGCAGCT CTATCAGTAC TCAAGGAAAC 
CACAAGAAAA ACATGAAAAT CAAAGCCGAT ATGGAAGTAC CAAAAGACTC CCTGGTAAAA 
GAGGCAAATG AAAACTTGCA AGAGGATGAA GACGATGCAG TTGCAGATTC TGTATTTCAG 
AGCCACATCA TAGAATCCAA CTGCCAGATG AGAACATTGG ACAGTGGGAT CGGAACCTTT 
CCACTCCCAG ACTCGGGAAA TCGCTCGACA GGACGCTACC TATGCCAGCC AGACTCCCCA 
GAGGACGCTG AGCCTCTCCT GCCTCTCCAG TCAGCCCTTT CTGCAGTTTC TTCCATGAGA 
GCCCAAACCC TTGAACGTGA AGTGCCTTCC TCCACAGACG GCCAGCGCCC TGCAGATAGC 
GCCATTGTTC ATTCCACATC CGACCCCATC ATGACCGCCA GAGGGATGAG GCCTCTTCAG 
AGCCGCCTCC CCAAACCAGC TTCCTCAGGA AAAGTCAGTT CCCAAAAGCA GAATGAAGCA 
GAGCCAAGGC CTCAGACATG CTCATCATTC GGATATGCTG AAGACCCAAT GGCAAGCCAG 
CCGCTTCCAG ACTGGGGGAG TGAAGTTGCT GCCACCGGGA CCCAGAAATT GAGACAACTT 
GAAGAAACAA AAGACGATCC CGAGAATAGA TTATCGAAAA TTTCCCTAGA GTCATTCAAT 
AAATTTAACA GCAATACTGT GATTTTATTA GAAAAAGAGA AGAACTCTCT GAACAAGGTT 
GAAGGACAGA AGGAAGAAAA AGAAAAAAAT GAAGAGACAT CTTTGAGTAG TTCAGATAGG 
CCTGGGGTAG ACAACTTGGA ATCTTTGAGT GATTCTTTAT ATGATAGCTT CTCTTCCTGT 
GCCAGTCAAG GTTCAAATGA TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509403.1
CDS	123..5909
Translation	MDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRMEETVRNLLQSQGSPEQKKEETVNIMVYQEKLSEEERKHKEALEDLHMVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_011511101.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511101.1

ORF Insert Sequence:

ATGGACTCCA ATAAATACAT TGAGCATCTG CTGACTCAGC TTGAGGAGCA ACACAGGAGT 
CTCTGGAGGG AGAAGTTGGC AGTGGCCCGA CTACAGCGAG AAGTTGCCCA AAGAACAAGT 
GAGGGGGCCA TGCATGAGAA GCTGATACAT GAACTGGAAG AGGAGAGACA CTTACGTCTT 
CAAAGCGAGA AGCGGTTGCA GGAGGTGACC CTAGAGTCTG AACGCAACAG AATTCAGATG 
CGTAGCTTGC AGCAGCAGTT CTCCAGGATG GAAGAAACAG TACGAAATCT ATTGCAGAGT 
CAAGGATCTC CAGAGCAGAA AAAAGAAGAA ACTGTTAATA TAATGGTCTA TCAGGAAAAG 
CTGTCAGAGG AAGAGAGAAA ACATAAGGAA GCTTTGGAAG ATCTTCACAT GGTGGTTGAT 
GAGGATTCGA GGAGTGAAAG CAGCAGTACA GATGAGGGAA AAGAAAAGAC CAAATTGCTA 
TTAGAGAGAT TGAAAGCTCT AGAGGCAGAG AATTCAGCGT TGGCTTTGGA GAATGAAAAT 
CAAAGGGAAC AATATGAGCG ATGTCTTGAT GAGGTAGCCA ACCAAGTTGT TCAAGCTCTG 
CTTACTCAAA AGGATCTAAG AGAGGAATGT GTGAAGTTGA AAACAAGAGT GTTTGATTTG 
GAACAGCAGA ATCGAACACT AAGCATCCTA TTCCAACAGC GAGTCAGACC CACTTCTGAT 
CTGCTCCTCC AGAAACTTCA CTCACGTCTC TTGGATCTTT CATCTGGAGA TTTGCTTTCA 
GAGGTGGAAA GAAACCGAAG TCTGACACAG TCACGAACTG ATGCTGAGGT GCACGAACAC 
CAACTAAATA CAAAATCGGC CTTGAAATGC CCTGGCTTGG GGGCTGTCAT CCCTGGTCAT 
CTCTGTCCTC GAAACAGCTA CAGTAGCAGC AGTGAACTGT CTCTTTCAAG CACCTGCAGC 
GAGTACTCCA GTGGCTCCTC CTACACGTGG CACGATGGGA AGAACCTCAG GAAAAGGCAA 
TCATCACAAA ACTGGGATAA AAGGCTAAGT ATTGATTCTT CGCTCCCAAG TGGCTTTGCT 
AGTCCTACAA ATGAACTACC TCCAACTCGT ATCAAGGAAA GCCACATTTT GGAAGGGCTA 
AGAAAGCTAC AGAAGCGAAA AGTGTTACTT GAACCCCCAT CAGTGATAAC CAAATGGGGT 
TATAAAGATT GCATGAACTC GAATGAAGGA ATATATTCTC CTGGAATTAA AAGTAGCAGC 
CTCAAGGAGT ATCCCCCCTG CAAAACAGCT GACCTGGGGA GCCCCTGCAA GGAACCCCAC 
AAGACATTTG TTTATGATCT AGATTCCCAC GTTGATGCGG ACGATGACCC TTCCACCTTA 
GCATTGCTCC AAGCAGTTCC AAACCAGAGC TGCAGGCCAC ATGGCAGTAA ATTAACCCAC 
AGTGTTTCCG ACAGTCTGTT TGGCTGGGAG ACAAACAGAA AACACTTTCT GGAAGGCACA 
TCCTCAGTTT ATCCCAAGGA AAGGCCTGAA AAGCTGACAA GTTGCGCCAG CAGCTGTCCC 
TTAGAGATGA AGCTGTGCCC AAGTGTGCAG ACGCCTCAGG TACAGAGGGA GAGGGGCCCA 
CAGGGCCAAG GCCATGGCCG CATGGCTCTC AACCTCCAGC TTTCAGACAC TGATGACAAT 
GAAACGTTTG ATGAGCTGCA CATAGAGAGC AGTGATGAGA AAAGTCCTTC AGACGTGTCA 
TTGGCTGCCG ACACCGATAA GTCCGTGGAG AACCTGGATG TCCTTGTGGG GTTTGGAAAA 
TCTCTATGTG GGTCTCCTGA AGAGGAGGAA AAACAAGTGC CCATCCCTTC AGAGACTAGG 
CCAAAGACTT TTAGTTTCAT TAAGCAGCAA AGAGTTGTAA AAAGGACTTC TTCAGAAGAA 
TGTGTGACTG TGATATTTGA TGCGGAAGAC GGTGAGCCCA TTGAATTCAG CTCTCACCAG 
ACTGGGGTTG TCACTGTTAC CAGAAATGAG ATTTCCATCA ATTCAACTCC TGCTGGACCC 
AAGGCAGAAC ATACTGAGCT TTTACCTCAG GGAATTGCTT GTTTACAGCC AAGAGCTGCT 
GCAAGAGACT ATACTTTCTT TAAAAGGTCT GAAGAGGACA CTGAGAAAAA CATTCCAAAA 
GATAATGTAG ATAATGTTCC CAGGGTGTCC ACTGAATCTT TCAGCTCCAG GACAGTGACA 
CAAAATCCTC AGCAGCAAAA GCTGGTCAAA CCAACACACA ATATATCATG CCAGAGTAAT 
TCCAGGTCTT CGGCACCCAT GGGCATCTAT CAAAAGCAAA ATCTGACAAA AATACCTCCC 
AGGGGCAAGT CTTCACCTCA GAAATCAAAA CTAATGGAGC CCGAAGCCAC CACACTACTC 
CCTTCATCTG GCCTGGTGAC TCTTGAAAAA TCACCAGCCT TAGCTCCTGG GAAACTCTCA 
CGATTCATGA AGACTGAGAG CTCAGGGCCC CTCTTTGAAT TACGATCAGA TCCACACATT 
CCAAAACATT CCGCCCAACT TCCGCACAGC TCCAGGATGC CCAGCAGGAG GGACTGGGTC 
CAGTGCCCCA AGAGTCAGAC TCCAGGGTCA CGGTCAAGGC CTGCCATTGA GTCTAGTGAC 
AGTGGAGAGC CCCCCACGAG GGATGAACAC TGTGGCTCTG GGCCGGAGGC AGGGGTGAAA 
TCCCCTTCCC CTCCGCCCCC TCCAGGCAGG TCCGTCTCCC TGCTGGCCAG GCCCAGCTAT 
GACTATTCAC CAGCACCTTC ATCCACCAAG TCCGAAACCA GGGTCCCCAG TGAAACAGCA 
AGGACCCCAT TCAAATCCCC GCTGCTGAAA GGAATTTCTG CTCCAGTTAT TTCTTCTAAT 
CCGGCCACGA CAGAAGTGCA GAGGAAGAAA CCTTCTGTGG CCTTCAAAAA GCCTATCTTC 
ACTCACCCTA TGCCCTCCCC AGAAGCAGTC ATTCAAACCC GATGCCCTGC TCATGCCCCC 
TCCAGCTCCT TCACCGTAAT GGCTCTGGGG CCTCCAAAGG TCTCTCCGAA GAGAGGTGTC 
CCCAAAACCT CTCCTCGCCA AACACTTGGG ACCCCACAAA GGGACATAGG ATTACAGACT 
CCCAGGATCT CTCCTTCAAC CCATGAGCCA CTGGAAATGA CGTCCTCCAA AAGTGTATCT 
CCAGGGAGAA AAGGACAATT GAATGATAGC GCCTCCACAC CCCCCAAGCC TTCCTTCTTA 
GGGGTAAATG AGTCACCATC ATCTCAGGTC AGCAGTTCCT CATCATCCTC ATCACCCGCC 
AAAAGCCATA ACAGCCCTCA TGGTTGTCAA AGTGCTCATG AGAAAGGACT GAAAACTCGC 
CTTCCAGTGG GACTCAAAGT GCTCATGAAG TCTCCCCAGC TGCTCAGGAA AAGTTCCACC 
GTGCCAGGGA AACATGAAAA AGACAGTTTA AATGAAGCCT CCAAAAGTTC CGTGGCTGTG 
AACAAGTCTA AGCCAGAGGA CTCCAAGAAT CCAGCAAGCA TGGAGATCAC AGCGGGTGAA 
AGAAATGTGA CCCTACCGGA TTCACAAGCA CAGGGCAGTT TAGCTGATGG GCTTCCCCTG 
GAAACAGCAC TACAAGAGCC ATTGGAAAGT AGCATCCCTG GGAGTGATGG AAGGGATGGG 
GTAGATAATA GATCCATGAG AAGATCCCTT TCCTCCAGCA AACCACACCT AAAACCAGCT 
CTGGGTATGA ATGGCGCCAA AGCCCGCAGC CACAGCTTCA GTACACACTC AGGAGACAAG 
CCTTCTACGC CCCCCATCGA AGGGTCAGGC AAAGTCCGCA CTCAGATCAT TACCAATACC 
GCCGAGAGAG GCAATTCTCT TACCCGGCAG AACTCTTCCA CGGAAAGCTC TCCCAACAAG 
GCCCCTTCTG CTCCCATGTT GGAGAGTCTC CCCAGTGTTG GGAGGCCCTC GGGGCACCCC 
TCCTCCGGGA AGGGCTCCCT GGGGAGCTCA GGCAGCTTCA GCAGTCAGCA TGGGAGCCCA 
AGTAAGTTGC CTTTGAGGAT CCCTCCAAAG TCTGAGGGAC TCCTCATCCC ACCTGGAAAG 
GAAGACCAGC AGGCCTTCAC CCAGGGAGAG TGCCCCAGTG CCAATGTGGC TGTACTTGGG 
GAACCAGGCA GTGACCGCCG CAGTTGCCCA CCCACCCCAA CAGACTGCCC TGAAGCCCTG 
CAGAGCCCAG GGAGGACTCA GCATCCAAGC ACTTTTGAAA CAAGCAGTAC ATCCAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCCTCT GCAACCGCGA CTGATGCTGT GAGTTCAGAA 
GCCCCCCTCT CACCCACAAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA 
AAGGGCCAAG TGCAAACAAA GCCCACCTCT GTGGAAGCAA AGCAGAAGCC TGGGCCTTCT 
TTTGCCAGCT GGTTTGGTTT TCGGAAGAGT AGACTTCCAG CTCTGAGTAG CAGGAAAATG 
GACATCTCCA AAACCAAAGT AGAAAAGAAA GATGCAAAAG TCTTGGGGTT TGGAAACAGA 
CAACTAAAAT CAGAAAGAAA AAAAGAGAAA AAGAAGCCTG AACTACAGTG TGAGACAGAA 
AATGAGCTTA TCAAGGACAC CAAGTCAGCA GATAATCCAG ATGGCGGTTT ACAAAGCAAA 
AATAATCGGA GAACACCACA AGACATTTAC AACCAACTGA AGATTGAACC AAGGAATAGA 
CACAGCCCTG TTGCATGTTC AACGAAAGAC ACCTTCATGA CGGAACTCTT GAACAGAGTT 
GATAAGAAAG CAGCTCCACA GACAGAAAGT GGATCAAGTA ATGCTTCCTG CAGGAATGTG 
TTAAAGGGCA GTTCTCAGGG CTCCTGTCTC ATCGGCAGCT CTATCAGTAC TCAAGGAAAC 
CACAAGAAAA ACATGAAAAT CAAAGCCGAT ATGGAAGTAC CAAAAGACTC CCTGGTAAAA 
GAGGCAAATG AAAACTTGCA AGAGGATGAA GACGATGCAG TTGCAGATTC TGTATTTCAG 
AGCCACATCA TAGAATCCAA CTGCCAGATG AGAACATTGG ACAGTGGGAT CGGAACCTTT 
CCACTCCCAG ACTCGGGAAA TCGCTCGACA GGACGCTACC TATGCCAGCC AGACTCCCCA 
GAGGACGCTG AGCCTCTCCT GCCTCTCCAG TCAGCCCTTT CTGCAGTTTC TTCCATGAGA 
GCCCAAACCC TTGAACGTGA AGTGCCTTCC TCCACAGACG GCCAGCGCCC TGCAGATAGC 
GCCATTGTTC ATTCCACATC CGACCCCATC ATGACCGCCA GAGGGATGAG GCCTCTTCAG 
AGCCGCCTCC CCAAACCAGC TTCCTCAGGA AAAGTCAGTT CCCAAAAGCA GAATGAAGCA 
GAGCCAAGGC CTCAGACATG CTCATCATTC GGATATGCTG AAGACCCAAT GGCAAGCCAG 
CCGCTTCCAG ACTGGGGGAG TGAAGTTGCT GCCACCGGGA CCCAGAAATT GAGACAACTT 
GAAGAAACAA AAGACGATCC CGAGAATAGA TTATCGAAAA TTTCCCTAGA GTCATTCAAT 
AAATTTAACA GCAATACTGT GATTTTATTA GAAAAAGAGA AGAACTCTCT GAACAAGGTT 
GAAGGACAGA AGGAAGAAAA AGAAAAAAAT GAAGAGACAT CTTTGAGTAG TTCAGATAGG 
CCTGGGGTAG ACAACTTGGA ATCTTTGAGT GATTCTTTAT ATGATAGCTT CTCTTCCTGT 
GCCAGTCAAG GTTCAAATGA TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_017003978.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859467.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X16, mRNA.

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CloneID	OHu89362
Clone ID Related Accession (Same CDS sequence)	XM_017003978.1 , XM_017003978.2
Accession Version	XM_017003978.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTTG ATGAGGATTC GAGGAGTGAA AGCAGCAGTA CAGATGAGGG AAAAGAAAAG 
ACCAAATTGC TATTAGAGAG ATTGAAAGCT CTAGAGGCAG AGAATTCAGC GTTGGCTTTG 
GAGAATGAAA ATCAAAGGGA ACAATATGAG CGATGTCTTG ATGAGGTAGC CAACCAAGTT 
GTTCAAGCTC TGCTTACTCA AAAGGATCTA AGAGAGGAAT GTGTGAAGTT GAAAACAAGA 
GTGTTTGATT TGGAACAGCA GAATCGAACA CTAAGCATCC TATTCCAACA GCGAGTCAGA 
CCCACTTCTG ATCTGCTCCT CCAGAAACTT CACTCACGTC TCTTGGATCT TTCATCTGGA 
GATTTGCTTT CAGAGGTGGA AAGAAACCGA AGTCTGACAC AGTCACGAAC TGATGCTGAG 
GTGCACGAAC ACCAACTAAA TACAAAATCG GCCTTGAAAT GCCCTGGCTT GGGGGCTGTC 
ATCCCTGGTC ATCTCTGTCC TCGAAACAGC TACAGTAGCA GCAGTGAACT GTCTCTTTCA 
AGCACCTGCA GCGAGTACTC CAGTGGCTCC TCCTACACGT GGCACGATGG GAAGAACCTC 
AGGAAAAGGC AATCATCACA AAACTGGGAT AAAAGGCTAA GTATTGATTC TTCGCTCCCA 
AGTGGCTTTG CTAGTCCTAC AAATGAACTA CCTCCAACTC GTATCAAGGA AAGCCACATT 
TTGGAAGGGC TAAGAAAGCT ACAGAAGCGA AAAGTGTTAC TTGAACCCCC ATCAGTGATA 
ACCAAATGGG GTTATAAAGA TTGCATGAAC TCGAATGAAG GAATATATTC TCCTGGAATT 
AAAAGTAGCA GCCTCAAGGA GTATCCCCCC TGCAAAACAG CTGACCTGGG GAGCCCCTGC 
AAGGAACCCC ACAAGACATT TGTTTATGAT CTAGATTCCC ACGTTGATGC GGACGATGAC 
CCTTCCACCT TAGCATTGCT CCAAGCAGTT CCAAACCAGA GCTGCAGGCC ACATGGCAGT 
AAATTAACCC ACAGTGTTTC CGACAGTCTG TTTGGCTGGG AGACAAACAG AAAACACTTT 
CTGGAAGGCA CATCCTCAGT TTATCCCAAG GAAAGGCCTG AAAAGCTGAC AAGTTGCGCC 
AGCAGCTGTC CCTTAGAGAT GAAGCTGTGC CCAAGTGTGC AGACGCCTCA GGTACAGAGG 
GAGAGGGGCC CACAGGGCCA AGGCCATGGC CGCATGGCTC TCAACCTCCA GCTTTCAGAC 
ACTGATGACA ATGAAACGTT TGATGAGCTG CACATAGAGA GCAGTGATGA GAAAAGTCCT 
TCAGACGTGT CATTGGCTGC CGACACCGAT AAGTCCGTGG AGAACCTGGA TGTCCTTGTG 
GGGTTTGGAA AATCTCTATG TGGGTCTCCT GAAGAGGAGG AAAAACAAGT GCCCATCCCT 
TCAGAGACTA GGCCAAAGAC TTTTAGTTTC ATTAAGCAGC AAAGAGTTGT AAAAAGGACT 
TCTTCAGAAG AATGTGTGAC TGTGATATTT GATGCGGAAG ACGGTGAGCC CATTGAATTC 
AGCTCTCACC AGACTGGGGT TGTCACTGTT ACCAGAAATG AGATTTCCAT CAATTCAACT 
CCTGCTGGAC CCAAGGCAGA ACATACTGAG CTTTTACCTC AGGGAATTGC TTGTTTACAG 
CCAAGAGCTG CTGCAAGAGA CTATACTTTC TTTAAAAGGT CTGAAGAGGA CACTGAGAAA 
AACATTCCAA AAGATAATGT AGATAATGTT CCCAGGGTGT CCACTGAATC TTTCAGCTCC 
AGGACAGTGA CACAAAATCC TCAGCAGCAA AAGCTGGTCA AACCAACACA CAATATATCA 
TGCCAGAGTA ATTCCAGGTC TTCGGCACCC ATGGGCATCT ATCAAAAGCA AAATCTGACA 
AAAATACCTC CCAGGGGCAA GTCTTCACCT CAGAAATCAA AACTAATGGA GCCCGAAGCC 
ACCACACTAC TCCCTTCATC TGGCCTGGTG ACTCTTGAAA AATCACCAGC CTTAGCTCCT 
GGGAAACTCT CACGATTCAT GAAGACTGAG AGCTCAGGGC CCCTCTTTGA ATTACGATCA 
GATCCACACA TTCCAAAACA TTCCGCCCAA CTTCCGCACA GCTCCAGGAT GCCCAGCAGG 
AGGGACTGGG TCCAGTGCCC CAAGAGTCAG ACTCCAGGGT CACGGTCAAG GCCTGCCATT 
GAGTCTAGTG ACAGTGGAGA GCCCCCCACG AGGGATGAAC ACTGTGGCTC TGGGCCGGAG 
GCAGGGGTGA AATCCCCTTC CCCTCCGCCC CCTCCAGGCA GGTCCGTCTC CCTGCTGGCC 
AGGCCCAGCT ATGACTATTC ACCAGCACCT TCATCCACCA AGTCCGAAAC CAGGGTCCCC 
AGTGAAACAG CAAGGACCCC ATTCAAATCC CCGCTGCTGA AAGGAATTTC TGCTCCAGTT 
ATTTCTTCTA ATCCGGCCAC GACAGAAGTG CAGAGGAAGA AACCTTCTGT GGCCTTCAAA 
AAGCCTATCT TCACTCACCC TATGCCCTCC CCAGAAGCAG TCATTCAAAC CCGATGCCCT 
GCTCATGCCC CCTCCAGCTC CTTCACCGTA ATGGCTCTGG GGCCTCCAAA GGTCTCTCCG 
AAGAGAGGTG TCCCCAAAAC CTCTCCTCGC CAAACACTTG GGACCCCACA AAGGGACATA 
GGATTACAGA CTCCCAGGAT CTCTCCTTCA ACCCATGAGC CACTGGAAAT GACGTCCTCC 
AAAAGTGTAT CTCCAGGGAG AAAAGGACAA TTGAATGATA GCGCCTCCAC ACCCCCCAAG 
CCTTCCTTCT TAGGGGTAAA TGAGTCACCA TCATCTCAGG TCAGCAGTTC CTCATCATCC 
TCATCACCCG CCAAAAGCCA TAACAGCCCT CATGGTTGTC AAAGTGCTCA TGAGAAAGGA 
CTGAAAACTC GCCTTCCAGT GGGACTCAAA GTGCTCATGA AGTCTCCCCA GCTGCTCAGG 
AAAAGTTCCA CCGTGCCAGG GAAACATGAA AAAGACAGTT TAAATGAAGC CTCCAAAAGT 
TCCGTGGCTG TGAACAAGTC TAAGCCAGAG GACTCCAAGA ATCCAGCAAG CATGGAGATC 
ACAGCGGGTG AAAGAAATGT GACCCTACCG GATTCACAAG CACAGGGCAG TTTAGCTGAT 
GGGCTTCCCC TGGAAACAGC ACTACAAGAG CCATTGGAAA GTAGCATCCC TGGGAGTGAT 
GGAAGGGATG GGGTAGATAA TAGATCCATG AGAAGATCCC TTTCCTCCAG CAAACCACAC 
CTAAAACCAG CTCTGGGTAT GAATGGCGCC AAAGCCCGCA GCCACAGCTT CAGTACACAC 
TCAGGAGACA AGCCTTCTAC GCCCCCCATC GAAGGGTCAG GCAAAGTCCG CACTCAGATC 
ATTACCAATA CCGCCGAGAG AGGCAATTCT CTTACCCGGC AGAACTCTTC CACGGAAAGC 
TCTCCCAACA AGGCCCCTTC TGCTCCCATG TTGGAGAGTC TCCCCAGTGT TGGGAGGCCC 
TCGGGGCACC CCTCCTCCGG GAAGGGCTCC CTGGGGAGCT CAGGCAGCTT CAGCAGTCAG 
CATGGGAGCC CAAGTAAGTT GCCTTTGAGG ATCCCTCCAA AGTCTGAGGG ACTCCTCATC 
CCACCTGGAA AGGAAGACCA GCAGGCCTTC ACCCAGGGAG AGTGCCCCAG TGCCAATGTG 
GCTGTACTTG GGGAACCAGG CAGTGACCGC CGCAGTTGCC CACCCACCCC AACAGACTGC 
CCTGAAGCCC TGCAGAGCCC AGGGAGGACT CAGCATCCAA GCACTTTTGA AACAAGCAGT 
ACATCCAAGC TAGAAACTTC TGGAAGGCAT CCAGATGCCT CTGCAACCGC GACTGATGCT 
GTGAGTTCAG AAGCCCCCCT CTCACCCACA ATCGAAGAAA AGGTCATGTT GTGCATTCAG 
GAAAATGTGG AAAAGGGCCA AGTGCAAACA AAGCCCACCT CTGTGGAAGC AAAGCAGAAG 
CCTGGGCCTT CTTTTGCCAG CTGGTTTGGT TTTCGGAAGA GTAGACTTCC AGCTCTGAGT 
AGCAGGAAAA TGGACATCTC CAAAACCAAA GTAGAAAAGA AAGATGCAAA AGTCTTGGGG 
TTTGGAAACA GACAACTAAA ATCAGAAAGA AAAAAAGAGA AAAAGAAGCC TGAACTACAG 
TGTGAGACAG AAAATGAGCT TATCAAGGAC ACCAAGTCAG CAGATAATCC AGATGGCGGT 
TTACAAAGCA AAAATAATCG GAGAACACCA CAAGACATTT ACAACCAACT GAAGATTGAA 
CCAAGGAATA GACACAGCCC TGTTGCATGT TCAACGAAAG ACACCTTCAT GACGGAACTC 
TTGAACAGAG TTGATAAGAA AGCAGCTCCA CAGACAGAAA GTGGATCAAG TAATGCTTCC 
TGCAGGAATG TGTTAAAGGG CAGTTCTCAG GGCTCCTGTC TCATCGGCAG CTCTATCAGT 
ACTCAAGGAA ACCACAAGAA AAACATGAAA ATCAAAGCCG ATATGGAAGT ACCAAAAGAC 
TCCCTGGTAA AAGAGGCAAA TGAAAACTTG CAAGAGGATG AAGACGATGC AGTTGCAGAT 
TCTGTATTTC AGAGCCACAT CATAGAATCC AACTGCCAGA TGAGAACATT GGACAGTGGG 
ATCGGAACCT TTCCACTCCC AGACTCGGGA AATCGCTCGA CAGGACGCTA CCTATGCCAG 
CCAGACTCCC CAGAGGACGC TGAGCCTCTC CTGCCTCTCC AGTCAGCCCT TTCTGCAGTT 
TCTTCCATGA GAGCCCAAAC CCTTGAACGT GAAGTGCCTT CCTCCACAGA CGGCCAGCGC 
CCTGCAGATA GCGCCATTGT TCATTCCACA TCCGACCCCA TCATGACCGC CAGAGGGATG 
AGGCCTCTTC AGAGCCGCCT CCCCAAACCA GCTTCCTCAG GAAAAGTCAG TTCCCAAAAG 
CAGAATGAAG CAGAGCCAAG GCCTCAGACA TGCTCATCAT TCGGATATGC TGAAGACCCA 
ATGGCAAGCC AGCCGCTTCC AGACTGGGGG AGTGAAGTTG CTGCCACCGG GACCCAGAAA 
TTGAGACAAC TTGAAGAAAC AAAAGACGAT CCCGAGAATA GATTATCGAA AATTTCCCTA 
GAGTCATTCA ATAAATTTAA CAGCAATACT GTGATTTTAT TAGAAAAAGA GAAGAACTCT 
CTGAACAAGG TTGAAGGACA GAAGGAAGAA AAAGAAAAAA ATGAAGAGAC ATCTTTGAGT 
AGTTCAGATA GGCCTGGGGT AGACAACTTG GAATCTTTGA GTGATTCTTT ATATGATAGC 
TTCTCTTCCT GTGCCAGTCA AGGTTCAAAT GATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859467.1
CDS	969..6347
Translation	MVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_017003978.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003978.1

ORF Insert Sequence:

ATGGTGGTTG ATGAGGATTC GAGGAGTGAA AGCAGCAGTA CAGATGAGGG AAAAGAAAAG 
ACCAAATTGC TATTAGAGAG ATTGAAAGCT CTAGAGGCAG AGAATTCAGC GTTGGCTTTG 
GAGAATGAAA ATCAAAGGGA ACAATATGAG CGATGTCTTG ATGAGGTAGC CAACCAAGTT 
GTTCAAGCTC TGCTTACTCA AAAGGATCTA AGAGAGGAAT GTGTGAAGTT GAAAACAAGA 
GTGTTTGATT TGGAACAGCA GAATCGAACA CTAAGCATCC TATTCCAACA GCGAGTCAGA 
CCCACTTCTG ATCTGCTCCT CCAGAAACTT CACTCACGTC TCTTGGATCT TTCATCTGGA 
GATTTGCTTT CAGAGGTGGA AAGAAACCGA AGTCTGACAC AGTCACGAAC TGATGCTGAG 
GTGCACGAAC ACCAACTAAA TACAAAATCG GCCTTGAAAT GCCCTGGCTT GGGGGCTGTC 
ATCCCTGGTC ATCTCTGTCC TCGAAACAGC TACAGTAGCA GCAGTGAACT GTCTCTTTCA 
AGCACCTGCA GCGAGTACTC CAGTGGCTCC TCCTACACGT GGCACGATGG GAAGAACCTC 
AGGAAAAGGC AATCATCACA AAACTGGGAT AAAAGGCTAA GTATTGATTC TTCGCTCCCA 
AGTGGCTTTG CTAGTCCTAC AAATGAACTA CCTCCAACTC GTATCAAGGA AAGCCACATT 
TTGGAAGGGC TAAGAAAGCT ACAGAAGCGA AAAGTGTTAC TTGAACCCCC ATCAGTGATA 
ACCAAATGGG GTTATAAAGA TTGCATGAAC TCGAATGAAG GAATATATTC TCCTGGAATT 
AAAAGTAGCA GCCTCAAGGA GTATCCCCCC TGCAAAACAG CTGACCTGGG GAGCCCCTGC 
AAGGAACCCC ACAAGACATT TGTTTATGAT CTAGATTCCC ACGTTGATGC GGACGATGAC 
CCTTCCACCT TAGCATTGCT CCAAGCAGTT CCAAACCAGA GCTGCAGGCC ACATGGCAGT 
AAATTAACCC ACAGTGTTTC CGACAGTCTG TTTGGCTGGG AGACAAACAG AAAACACTTT 
CTGGAAGGCA CATCCTCAGT TTATCCCAAG GAAAGGCCTG AAAAGCTGAC AAGTTGCGCC 
AGCAGCTGTC CCTTAGAGAT GAAGCTGTGC CCAAGTGTGC AGACGCCTCA GGTACAGAGG 
GAGAGGGGCC CACAGGGCCA AGGCCATGGC CGCATGGCTC TCAACCTCCA GCTTTCAGAC 
ACTGATGACA ATGAAACGTT TGATGAGCTG CACATAGAGA GCAGTGATGA GAAAAGTCCT 
TCAGACGTGT CATTGGCTGC CGACACCGAT AAGTCCGTGG AGAACCTGGA TGTCCTTGTG 
GGGTTTGGAA AATCTCTATG TGGGTCTCCT GAAGAGGAGG AAAAACAAGT GCCCATCCCT 
TCAGAGACTA GGCCAAAGAC TTTTAGTTTC ATTAAGCAGC AAAGAGTTGT AAAAAGGACT 
TCTTCAGAAG AATGTGTGAC TGTGATATTT GATGCGGAAG ACGGTGAGCC CATTGAATTC 
AGCTCTCACC AGACTGGGGT TGTCACTGTT ACCAGAAATG AGATTTCCAT CAATTCAACT 
CCTGCTGGAC CCAAGGCAGA ACATACTGAG CTTTTACCTC AGGGAATTGC TTGTTTACAG 
CCAAGAGCTG CTGCAAGAGA CTATACTTTC TTTAAAAGGT CTGAAGAGGA CACTGAGAAA 
AACATTCCAA AAGATAATGT AGATAATGTT CCCAGGGTGT CCACTGAATC TTTCAGCTCC 
AGGACAGTGA CACAAAATCC TCAGCAGCAA AAGCTGGTCA AACCAACACA CAATATATCA 
TGCCAGAGTA ATTCCAGGTC TTCGGCACCC ATGGGCATCT ATCAAAAGCA AAATCTGACA 
AAAATACCTC CCAGGGGCAA GTCTTCACCT CAGAAATCAA AACTAATGGA GCCCGAAGCC 
ACCACACTAC TCCCTTCATC TGGCCTGGTG ACTCTTGAAA AATCACCAGC CTTAGCTCCT 
GGGAAACTCT CACGATTCAT GAAGACTGAG AGCTCAGGGC CCCTCTTTGA ATTACGATCA 
GATCCACACA TTCCAAAACA TTCCGCCCAA CTTCCGCACA GCTCCAGGAT GCCCAGCAGG 
AGGGACTGGG TCCAGTGCCC CAAGAGTCAG ACTCCAGGGT CACGGTCAAG GCCTGCCATT 
GAGTCTAGTG ACAGTGGAGA GCCCCCCACG AGGGATGAAC ACTGTGGCTC TGGGCCGGAG 
GCAGGGGTGA AATCCCCTTC CCCTCCGCCC CCTCCAGGCA GGTCCGTCTC CCTGCTGGCC 
AGGCCCAGCT ATGACTATTC ACCAGCACCT TCATCCACCA AGTCCGAAAC CAGGGTCCCC 
AGTGAAACAG CAAGGACCCC ATTCAAATCC CCGCTGCTGA AAGGAATTTC TGCTCCAGTT 
ATTTCTTCTA ATCCGGCCAC GACAGAAGTG CAGAGGAAGA AACCTTCTGT GGCCTTCAAA 
AAGCCTATCT TCACTCACCC TATGCCCTCC CCAGAAGCAG TCATTCAAAC CCGATGCCCT 
GCTCATGCCC CCTCCAGCTC CTTCACCGTA ATGGCTCTGG GGCCTCCAAA GGTCTCTCCG 
AAGAGAGGTG TCCCCAAAAC CTCTCCTCGC CAAACACTTG GGACCCCACA AAGGGACATA 
GGATTACAGA CTCCCAGGAT CTCTCCTTCA ACCCATGAGC CACTGGAAAT GACGTCCTCC 
AAAAGTGTAT CTCCAGGGAG AAAAGGACAA TTGAATGATA GCGCCTCCAC ACCCCCCAAG 
CCTTCCTTCT TAGGGGTAAA TGAGTCACCA TCATCTCAGG TCAGCAGTTC CTCATCATCC 
TCATCACCCG CCAAAAGCCA TAACAGCCCT CATGGTTGTC AAAGTGCTCA TGAGAAAGGA 
CTGAAAACTC GCCTTCCAGT GGGACTCAAA GTGCTCATGA AGTCTCCCCA GCTGCTCAGG 
AAAAGTTCCA CCGTGCCAGG GAAACATGAA AAAGACAGTT TAAATGAAGC CTCCAAAAGT 
TCCGTGGCTG TGAACAAGTC TAAGCCAGAG GACTCCAAGA ATCCAGCAAG CATGGAGATC 
ACAGCGGGTG AAAGAAATGT GACCCTACCG GATTCACAAG CACAGGGCAG TTTAGCTGAT 
GGGCTTCCCC TGGAAACAGC ACTACAAGAG CCATTGGAAA GTAGCATCCC TGGGAGTGAT 
GGAAGGGATG GGGTAGATAA TAGATCCATG AGAAGATCCC TTTCCTCCAG CAAACCACAC 
CTAAAACCAG CTCTGGGTAT GAATGGCGCC AAAGCCCGCA GCCACAGCTT CAGTACACAC 
TCAGGAGACA AGCCTTCTAC GCCCCCCATC GAAGGGTCAG GCAAAGTCCG CACTCAGATC 
ATTACCAATA CCGCCGAGAG AGGCAATTCT CTTACCCGGC AGAACTCTTC CACGGAAAGC 
TCTCCCAACA AGGCCCCTTC TGCTCCCATG TTGGAGAGTC TCCCCAGTGT TGGGAGGCCC 
TCGGGGCACC CCTCCTCCGG GAAGGGCTCC CTGGGGAGCT CAGGCAGCTT CAGCAGTCAG 
CATGGGAGCC CAAGTAAGTT GCCTTTGAGG ATCCCTCCAA AGTCTGAGGG ACTCCTCATC 
CCACCTGGAA AGGAAGACCA GCAGGCCTTC ACCCAGGGAG AGTGCCCCAG TGCCAATGTG 
GCTGTACTTG GGGAACCAGG CAGTGACCGC CGCAGTTGCC CACCCACCCC AACAGACTGC 
CCTGAAGCCC TGCAGAGCCC AGGGAGGACT CAGCATCCAA GCACTTTTGA AACAAGCAGT 
ACATCCAAGC TAGAAACTTC TGGAAGGCAT CCAGATGCCT CTGCAACCGC GACTGATGCT 
GTGAGTTCAG AAGCCCCCCT CTCACCCACA ATCGAAGAAA AGGTCATGTT GTGCATTCAG 
GAAAATGTGG AAAAGGGCCA AGTGCAAACA AAGCCCACCT CTGTGGAAGC AAAGCAGAAG 
CCTGGGCCTT CTTTTGCCAG CTGGTTTGGT TTTCGGAAGA GTAGACTTCC AGCTCTGAGT 
AGCAGGAAAA TGGACATCTC CAAAACCAAA GTAGAAAAGA AAGATGCAAA AGTCTTGGGG 
TTTGGAAACA GACAACTAAA ATCAGAAAGA AAAAAAGAGA AAAAGAAGCC TGAACTACAG 
TGTGAGACAG AAAATGAGCT TATCAAGGAC ACCAAGTCAG CAGATAATCC AGATGGCGGT 
TTACAAAGCA AAAATAATCG GAGAACACCA CAAGACATTT ACAACCAACT GAAGATTGAA 
CCAAGGAATA GACACAGCCC TGTTGCATGT TCAACGAAAG ACACCTTCAT GACGGAACTC 
TTGAACAGAG TTGATAAGAA AGCAGCTCCA CAGACAGAAA GTGGATCAAG TAATGCTTCC 
TGCAGGAATG TGTTAAAGGG CAGTTCTCAG GGCTCCTGTC TCATCGGCAG CTCTATCAGT 
ACTCAAGGAA ACCACAAGAA AAACATGAAA ATCAAAGCCG ATATGGAAGT ACCAAAAGAC 
TCCCTGGTAA AAGAGGCAAA TGAAAACTTG CAAGAGGATG AAGACGATGC AGTTGCAGAT 
TCTGTATTTC AGAGCCACAT CATAGAATCC AACTGCCAGA TGAGAACATT GGACAGTGGG 
ATCGGAACCT TTCCACTCCC AGACTCGGGA AATCGCTCGA CAGGACGCTA CCTATGCCAG 
CCAGACTCCC CAGAGGACGC TGAGCCTCTC CTGCCTCTCC AGTCAGCCCT TTCTGCAGTT 
TCTTCCATGA GAGCCCAAAC CCTTGAACGT GAAGTGCCTT CCTCCACAGA CGGCCAGCGC 
CCTGCAGATA GCGCCATTGT TCATTCCACA TCCGACCCCA TCATGACCGC CAGAGGGATG 
AGGCCTCTTC AGAGCCGCCT CCCCAAACCA GCTTCCTCAG GAAAAGTCAG TTCCCAAAAG 
CAGAATGAAG CAGAGCCAAG GCCTCAGACA TGCTCATCAT TCGGATATGC TGAAGACCCA 
ATGGCAAGCC AGCCGCTTCC AGACTGGGGG AGTGAAGTTG CTGCCACCGG GACCCAGAAA 
TTGAGACAAC TTGAAGAAAC AAAAGACGAT CCCGAGAATA GATTATCGAA AATTTCCCTA 
GAGTCATTCA ATAAATTTAA CAGCAATACT GTGATTTTAT TAGAAAAAGA GAAGAACTCT 
CTGAACAAGG TTGAAGGACA GAAGGAAGAA AAAGAAAAAA ATGAAGAGAC ATCTTTGAGT 
AGTTCAGATA GGCCTGGGGT AGACAACTTG GAATCTTTGA GTGATTCTTT ATATGATAGC 
TTCTCTTCCT GTGCCAGTCA AGGTTCAAAT GATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_017003978.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859467.1 Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X15, mRNA.

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CloneID	OHu89362
Clone ID Related Accession (Same CDS sequence)	XM_017003978.1 , XM_017003978.2
Accession Version	XM_017003978.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017003978.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTTG ATGAGGATTC GAGGAGTGAA AGCAGCAGTA CAGATGAGGG AAAAGAAAAG 
ACCAAATTGC TATTAGAGAG ATTGAAAGCT CTAGAGGCAG AGAATTCAGC GTTGGCTTTG 
GAGAATGAAA ATCAAAGGGA ACAATATGAG CGATGTCTTG ATGAGGTAGC CAACCAAGTT 
GTTCAAGCTC TGCTTACTCA AAAGGATCTA AGAGAGGAAT GTGTGAAGTT GAAAACAAGA 
GTGTTTGATT TGGAACAGCA GAATCGAACA CTAAGCATCC TATTCCAACA GCGAGTCAGA 
CCCACTTCTG ATCTGCTCCT CCAGAAACTT CACTCACGTC TCTTGGATCT TTCATCTGGA 
GATTTGCTTT CAGAGGTGGA AAGAAACCGA AGTCTGACAC AGTCACGAAC TGATGCTGAG 
GTGCACGAAC ACCAACTAAA TACAAAATCG GCCTTGAAAT GCCCTGGCTT GGGGGCTGTC 
ATCCCTGGTC ATCTCTGTCC TCGAAACAGC TACAGTAGCA GCAGTGAACT GTCTCTTTCA 
AGCACCTGCA GCGAGTACTC CAGTGGCTCC TCCTACACGT GGCACGATGG GAAGAACCTC 
AGGAAAAGGC AATCATCACA AAACTGGGAT AAAAGGCTAA GTATTGATTC TTCGCTCCCA 
AGTGGCTTTG CTAGTCCTAC AAATGAACTA CCTCCAACTC GTATCAAGGA AAGCCACATT 
TTGGAAGGGC TAAGAAAGCT ACAGAAGCGA AAAGTGTTAC TTGAACCCCC ATCAGTGATA 
ACCAAATGGG GTTATAAAGA TTGCATGAAC TCGAATGAAG GAATATATTC TCCTGGAATT 
AAAAGTAGCA GCCTCAAGGA GTATCCCCCC TGCAAAACAG CTGACCTGGG GAGCCCCTGC 
AAGGAACCCC ACAAGACATT TGTTTATGAT CTAGATTCCC ACGTTGATGC GGACGATGAC 
CCTTCCACCT TAGCATTGCT CCAAGCAGTT CCAAACCAGA GCTGCAGGCC ACATGGCAGT 
AAATTAACCC ACAGTGTTTC CGACAGTCTG TTTGGCTGGG AGACAAACAG AAAACACTTT 
CTGGAAGGCA CATCCTCAGT TTATCCCAAG GAAAGGCCTG AAAAGCTGAC AAGTTGCGCC 
AGCAGCTGTC CCTTAGAGAT GAAGCTGTGC CCAAGTGTGC AGACGCCTCA GGTACAGAGG 
GAGAGGGGCC CACAGGGCCA AGGCCATGGC CGCATGGCTC TCAACCTCCA GCTTTCAGAC 
ACTGATGACA ATGAAACGTT TGATGAGCTG CACATAGAGA GCAGTGATGA GAAAAGTCCT 
TCAGACGTGT CATTGGCTGC CGACACCGAT AAGTCCGTGG AGAACCTGGA TGTCCTTGTG 
GGGTTTGGAA AATCTCTATG TGGGTCTCCT GAAGAGGAGG AAAAACAAGT GCCCATCCCT 
TCAGAGACTA GGCCAAAGAC TTTTAGTTTC ATTAAGCAGC AAAGAGTTGT AAAAAGGACT 
TCTTCAGAAG AATGTGTGAC TGTGATATTT GATGCGGAAG ACGGTGAGCC CATTGAATTC 
AGCTCTCACC AGACTGGGGT TGTCACTGTT ACCAGAAATG AGATTTCCAT CAATTCAACT 
CCTGCTGGAC CCAAGGCAGA ACATACTGAG CTTTTACCTC AGGGAATTGC TTGTTTACAG 
CCAAGAGCTG CTGCAAGAGA CTATACTTTC TTTAAAAGGT CTGAAGAGGA CACTGAGAAA 
AACATTCCAA AAGATAATGT AGATAATGTT CCCAGGGTGT CCACTGAATC TTTCAGCTCC 
AGGACAGTGA CACAAAATCC TCAGCAGCAA AAGCTGGTCA AACCAACACA CAATATATCA 
TGCCAGAGTA ATTCCAGGTC TTCGGCACCC ATGGGCATCT ATCAAAAGCA AAATCTGACA 
AAAATACCTC CCAGGGGCAA GTCTTCACCT CAGAAATCAA AACTAATGGA GCCCGAAGCC 
ACCACACTAC TCCCTTCATC TGGCCTGGTG ACTCTTGAAA AATCACCAGC CTTAGCTCCT 
GGGAAACTCT CACGATTCAT GAAGACTGAG AGCTCAGGGC CCCTCTTTGA ATTACGATCA 
GATCCACACA TTCCAAAACA TTCCGCCCAA CTTCCGCACA GCTCCAGGAT GCCCAGCAGG 
AGGGACTGGG TCCAGTGCCC CAAGAGTCAG ACTCCAGGGT CACGGTCAAG GCCTGCCATT 
GAGTCTAGTG ACAGTGGAGA GCCCCCCACG AGGGATGAAC ACTGTGGCTC TGGGCCGGAG 
GCAGGGGTGA AATCCCCTTC CCCTCCGCCC CCTCCAGGCA GGTCCGTCTC CCTGCTGGCC 
AGGCCCAGCT ATGACTATTC ACCAGCACCT TCATCCACCA AGTCCGAAAC CAGGGTCCCC 
AGTGAAACAG CAAGGACCCC ATTCAAATCC CCGCTGCTGA AAGGAATTTC TGCTCCAGTT 
ATTTCTTCTA ATCCGGCCAC GACAGAAGTG CAGAGGAAGA AACCTTCTGT GGCCTTCAAA 
AAGCCTATCT TCACTCACCC TATGCCCTCC CCAGAAGCAG TCATTCAAAC CCGATGCCCT 
GCTCATGCCC CCTCCAGCTC CTTCACCGTA ATGGCTCTGG GGCCTCCAAA GGTCTCTCCG 
AAGAGAGGTG TCCCCAAAAC CTCTCCTCGC CAAACACTTG GGACCCCACA AAGGGACATA 
GGATTACAGA CTCCCAGGAT CTCTCCTTCA ACCCATGAGC CACTGGAAAT GACGTCCTCC 
AAAAGTGTAT CTCCAGGGAG AAAAGGACAA TTGAATGATA GCGCCTCCAC ACCCCCCAAG 
CCTTCCTTCT TAGGGGTAAA TGAGTCACCA TCATCTCAGG TCAGCAGTTC CTCATCATCC 
TCATCACCCG CCAAAAGCCA TAACAGCCCT CATGGTTGTC AAAGTGCTCA TGAGAAAGGA 
CTGAAAACTC GCCTTCCAGT GGGACTCAAA GTGCTCATGA AGTCTCCCCA GCTGCTCAGG 
AAAAGTTCCA CCGTGCCAGG GAAACATGAA AAAGACAGTT TAAATGAAGC CTCCAAAAGT 
TCCGTGGCTG TGAACAAGTC TAAGCCAGAG GACTCCAAGA ATCCAGCAAG CATGGAGATC 
ACAGCGGGTG AAAGAAATGT GACCCTACCG GATTCACAAG CACAGGGCAG TTTAGCTGAT 
GGGCTTCCCC TGGAAACAGC ACTACAAGAG CCATTGGAAA GTAGCATCCC TGGGAGTGAT 
GGAAGGGATG GGGTAGATAA TAGATCCATG AGAAGATCCC TTTCCTCCAG CAAACCACAC 
CTAAAACCAG CTCTGGGTAT GAATGGCGCC AAAGCCCGCA GCCACAGCTT CAGTACACAC 
TCAGGAGACA AGCCTTCTAC GCCCCCCATC GAAGGGTCAG GCAAAGTCCG CACTCAGATC 
ATTACCAATA CCGCCGAGAG AGGCAATTCT CTTACCCGGC AGAACTCTTC CACGGAAAGC 
TCTCCCAACA AGGCCCCTTC TGCTCCCATG TTGGAGAGTC TCCCCAGTGT TGGGAGGCCC 
TCGGGGCACC CCTCCTCCGG GAAGGGCTCC CTGGGGAGCT CAGGCAGCTT CAGCAGTCAG 
CATGGGAGCC CAAGTAAGTT GCCTTTGAGG ATCCCTCCAA AGTCTGAGGG ACTCCTCATC 
CCACCTGGAA AGGAAGACCA GCAGGCCTTC ACCCAGGGAG AGTGCCCCAG TGCCAATGTG 
GCTGTACTTG GGGAACCAGG CAGTGACCGC CGCAGTTGCC CACCCACCCC AACAGACTGC 
CCTGAAGCCC TGCAGAGCCC AGGGAGGACT CAGCATCCAA GCACTTTTGA AACAAGCAGT 
ACATCCAAGC TAGAAACTTC TGGAAGGCAT CCAGATGCCT CTGCAACCGC GACTGATGCT 
GTGAGTTCAG AAGCCCCCCT CTCACCCACA ATCGAAGAAA AGGTCATGTT GTGCATTCAG 
GAAAATGTGG AAAAGGGCCA AGTGCAAACA AAGCCCACCT CTGTGGAAGC AAAGCAGAAG 
CCTGGGCCTT CTTTTGCCAG CTGGTTTGGT TTTCGGAAGA GTAGACTTCC AGCTCTGAGT 
AGCAGGAAAA TGGACATCTC CAAAACCAAA GTAGAAAAGA AAGATGCAAA AGTCTTGGGG 
TTTGGAAACA GACAACTAAA ATCAGAAAGA AAAAAAGAGA AAAAGAAGCC TGAACTACAG 
TGTGAGACAG AAAATGAGCT TATCAAGGAC ACCAAGTCAG CAGATAATCC AGATGGCGGT 
TTACAAAGCA AAAATAATCG GAGAACACCA CAAGACATTT ACAACCAACT GAAGATTGAA 
CCAAGGAATA GACACAGCCC TGTTGCATGT TCAACGAAAG ACACCTTCAT GACGGAACTC 
TTGAACAGAG TTGATAAGAA AGCAGCTCCA CAGACAGAAA GTGGATCAAG TAATGCTTCC 
TGCAGGAATG TGTTAAAGGG CAGTTCTCAG GGCTCCTGTC TCATCGGCAG CTCTATCAGT 
ACTCAAGGAA ACCACAAGAA AAACATGAAA ATCAAAGCCG ATATGGAAGT ACCAAAAGAC 
TCCCTGGTAA AAGAGGCAAA TGAAAACTTG CAAGAGGATG AAGACGATGC AGTTGCAGAT 
TCTGTATTTC AGAGCCACAT CATAGAATCC AACTGCCAGA TGAGAACATT GGACAGTGGG 
ATCGGAACCT TTCCACTCCC AGACTCGGGA AATCGCTCGA CAGGACGCTA CCTATGCCAG 
CCAGACTCCC CAGAGGACGC TGAGCCTCTC CTGCCTCTCC AGTCAGCCCT TTCTGCAGTT 
TCTTCCATGA GAGCCCAAAC CCTTGAACGT GAAGTGCCTT CCTCCACAGA CGGCCAGCGC 
CCTGCAGATA GCGCCATTGT TCATTCCACA TCCGACCCCA TCATGACCGC CAGAGGGATG 
AGGCCTCTTC AGAGCCGCCT CCCCAAACCA GCTTCCTCAG GAAAAGTCAG TTCCCAAAAG 
CAGAATGAAG CAGAGCCAAG GCCTCAGACA TGCTCATCAT TCGGATATGC TGAAGACCCA 
ATGGCAAGCC AGCCGCTTCC AGACTGGGGG AGTGAAGTTG CTGCCACCGG GACCCAGAAA 
TTGAGACAAC TTGAAGAAAC AAAAGACGAT CCCGAGAATA GATTATCGAA AATTTCCCTA 
GAGTCATTCA ATAAATTTAA CAGCAATACT GTGATTTTAT TAGAAAAAGA GAAGAACTCT 
CTGAACAAGG TTGAAGGACA GAAGGAAGAA AAAGAAAAAA ATGAAGAGAC ATCTTTGAGT 
AGTTCAGATA GGCCTGGGGT AGACAACTTG GAATCTTTGA GTGATTCTTT ATATGATAGC 
TTCTCTTCCT GTGCCAGTCA AGGTTCAAAT GATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859467.1
CDS	971..6349
Translation	MVVDEDSRSESSSTDEGKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECVKLKTRVFDLEQQNRTLSILFQQRVRPTSDLLLQKLHSRLLDLSSGDLLSEVERNRSLTQSRTDAEVHEHQLNTKSALKCPGLGAVIPGHLCPRNSYSSSSELSLSSTCSEYSSGSSYTWHDGKNLRKRQSSQNWDKRLSIDSSLPSGFASPTNELPPTRIKESHILEGLRKLQKRKVLLEPPSVITKWGYKDCMNSNEGIYSPGIKSSSLKEYPPCKTADLGSPCKEPHKTFVYDLDSHVDADDDPSTLALLQAVPNQSCRPHGSKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKLCPSVQTPQVQRERGPQGQGHGRMALNLQLSDTDDNETFDELHIESSDEKSPSDVSLAADTDKSVENLDVLVGFGKSLCGSPEEEEKQVPIPSETRPKTFSFIKQQRVVKRTSSEECVTVIFDAEDGEPIEFSSHQTGVVTVTRNEISINSTPAGPKAEHTELLPQGIACLQPRAAARDYTFFKRSEEDTEKNIPKDNVDNVPRVSTESFSSRTVTQNPQQQKLVKPTHNISCQSNSRSSAPMGIYQKQNLTKIPPRGKSSPQKSKLMEPEATTLLPSSGLVTLEKSPALAPGKLSRFMKTESSGPLFELRSDPHIPKHSAQLPHSSRMPSRRDWVQCPKSQTPGSRSRPAIESSDSGEPPTRDEHCGSGPEAGVKSPSPPPPPGRSVSLLARPSYDYSPAPSSTKSETRVPSETARTPFKSPLLKGISAPVISSNPATTEVQRKKPSVAFKKPIFTHPMPSPEAVIQTRCPAHAPSSSFTVMALGPPKVSPKRGVPKTSPRQTLGTPQRDIGLQTPRISPSTHEPLEMTSSKSVSPGRKGQLNDSASTPPKPSFLGVNESPSSQVSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQLLRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGSLADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHSFSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLPSVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGECPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDASATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFRKSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANENLQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAEPLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRLPKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQKLRQLEETKDDPENRLSKISLESFNKFNSNTVILLEKEKNSLNKVEGQKEEKEKNEETSLSSSDRPGVDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_017003978.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 5 (NCKAP5), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003978.2

ORF Insert Sequence:

ATGGTGGTTG ATGAGGATTC GAGGAGTGAA AGCAGCAGTA CAGATGAGGG AAAAGAAAAG 
ACCAAATTGC TATTAGAGAG ATTGAAAGCT CTAGAGGCAG AGAATTCAGC GTTGGCTTTG 
GAGAATGAAA ATCAAAGGGA ACAATATGAG CGATGTCTTG ATGAGGTAGC CAACCAAGTT 
GTTCAAGCTC TGCTTACTCA AAAGGATCTA AGAGAGGAAT GTGTGAAGTT GAAAACAAGA 
GTGTTTGATT TGGAACAGCA GAATCGAACA CTAAGCATCC TATTCCAACA GCGAGTCAGA 
CCCACTTCTG ATCTGCTCCT CCAGAAACTT CACTCACGTC TCTTGGATCT TTCATCTGGA 
GATTTGCTTT CAGAGGTGGA AAGAAACCGA AGTCTGACAC AGTCACGAAC TGATGCTGAG 
GTGCACGAAC ACCAACTAAA TACAAAATCG GCCTTGAAAT GCCCTGGCTT GGGGGCTGTC 
ATCCCTGGTC ATCTCTGTCC TCGAAACAGC TACAGTAGCA GCAGTGAACT GTCTCTTTCA 
AGCACCTGCA GCGAGTACTC CAGTGGCTCC TCCTACACGT GGCACGATGG GAAGAACCTC 
AGGAAAAGGC AATCATCACA AAACTGGGAT AAAAGGCTAA GTATTGATTC TTCGCTCCCA 
AGTGGCTTTG CTAGTCCTAC AAATGAACTA CCTCCAACTC GTATCAAGGA AAGCCACATT 
TTGGAAGGGC TAAGAAAGCT ACAGAAGCGA AAAGTGTTAC TTGAACCCCC ATCAGTGATA 
ACCAAATGGG GTTATAAAGA TTGCATGAAC TCGAATGAAG GAATATATTC TCCTGGAATT 
AAAAGTAGCA GCCTCAAGGA GTATCCCCCC TGCAAAACAG CTGACCTGGG GAGCCCCTGC 
AAGGAACCCC ACAAGACATT TGTTTATGAT CTAGATTCCC ACGTTGATGC GGACGATGAC 
CCTTCCACCT TAGCATTGCT CCAAGCAGTT CCAAACCAGA GCTGCAGGCC ACATGGCAGT 
AAATTAACCC ACAGTGTTTC CGACAGTCTG TTTGGCTGGG AGACAAACAG AAAACACTTT 
CTGGAAGGCA CATCCTCAGT TTATCCCAAG GAAAGGCCTG AAAAGCTGAC AAGTTGCGCC 
AGCAGCTGTC CCTTAGAGAT GAAGCTGTGC CCAAGTGTGC AGACGCCTCA GGTACAGAGG 
GAGAGGGGCC CACAGGGCCA AGGCCATGGC CGCATGGCTC TCAACCTCCA GCTTTCAGAC 
ACTGATGACA ATGAAACGTT TGATGAGCTG CACATAGAGA GCAGTGATGA GAAAAGTCCT 
TCAGACGTGT CATTGGCTGC CGACACCGAT AAGTCCGTGG AGAACCTGGA TGTCCTTGTG 
GGGTTTGGAA AATCTCTATG TGGGTCTCCT GAAGAGGAGG AAAAACAAGT GCCCATCCCT 
TCAGAGACTA GGCCAAAGAC TTTTAGTTTC ATTAAGCAGC AAAGAGTTGT AAAAAGGACT 
TCTTCAGAAG AATGTGTGAC TGTGATATTT GATGCGGAAG ACGGTGAGCC CATTGAATTC 
AGCTCTCACC AGACTGGGGT TGTCACTGTT ACCAGAAATG AGATTTCCAT CAATTCAACT 
CCTGCTGGAC CCAAGGCAGA ACATACTGAG CTTTTACCTC AGGGAATTGC TTGTTTACAG 
CCAAGAGCTG CTGCAAGAGA CTATACTTTC TTTAAAAGGT CTGAAGAGGA CACTGAGAAA 
AACATTCCAA AAGATAATGT AGATAATGTT CCCAGGGTGT CCACTGAATC TTTCAGCTCC 
AGGACAGTGA CACAAAATCC TCAGCAGCAA AAGCTGGTCA AACCAACACA CAATATATCA 
TGCCAGAGTA ATTCCAGGTC TTCGGCACCC ATGGGCATCT ATCAAAAGCA AAATCTGACA 
AAAATACCTC CCAGGGGCAA GTCTTCACCT CAGAAATCAA AACTAATGGA GCCCGAAGCC 
ACCACACTAC TCCCTTCATC TGGCCTGGTG ACTCTTGAAA AATCACCAGC CTTAGCTCCT 
GGGAAACTCT CACGATTCAT GAAGACTGAG AGCTCAGGGC CCCTCTTTGA ATTACGATCA 
GATCCACACA TTCCAAAACA TTCCGCCCAA CTTCCGCACA GCTCCAGGAT GCCCAGCAGG 
AGGGACTGGG TCCAGTGCCC CAAGAGTCAG ACTCCAGGGT CACGGTCAAG GCCTGCCATT 
GAGTCTAGTG ACAGTGGAGA GCCCCCCACG AGGGATGAAC ACTGTGGCTC TGGGCCGGAG 
GCAGGGGTGA AATCCCCTTC CCCTCCGCCC CCTCCAGGCA GGTCCGTCTC CCTGCTGGCC 
AGGCCCAGCT ATGACTATTC ACCAGCACCT TCATCCACCA AGTCCGAAAC CAGGGTCCCC 
AGTGAAACAG CAAGGACCCC ATTCAAATCC CCGCTGCTGA AAGGAATTTC TGCTCCAGTT 
ATTTCTTCTA ATCCGGCCAC GACAGAAGTG CAGAGGAAGA AACCTTCTGT GGCCTTCAAA 
AAGCCTATCT TCACTCACCC TATGCCCTCC CCAGAAGCAG TCATTCAAAC CCGATGCCCT 
GCTCATGCCC CCTCCAGCTC CTTCACCGTA ATGGCTCTGG GGCCTCCAAA GGTCTCTCCG 
AAGAGAGGTG TCCCCAAAAC CTCTCCTCGC CAAACACTTG GGACCCCACA AAGGGACATA 
GGATTACAGA CTCCCAGGAT CTCTCCTTCA ACCCATGAGC CACTGGAAAT GACGTCCTCC 
AAAAGTGTAT CTCCAGGGAG AAAAGGACAA TTGAATGATA GCGCCTCCAC ACCCCCCAAG 
CCTTCCTTCT TAGGGGTAAA TGAGTCACCA TCATCTCAGG TCAGCAGTTC CTCATCATCC 
TCATCACCCG CCAAAAGCCA TAACAGCCCT CATGGTTGTC AAAGTGCTCA TGAGAAAGGA 
CTGAAAACTC GCCTTCCAGT GGGACTCAAA GTGCTCATGA AGTCTCCCCA GCTGCTCAGG 
AAAAGTTCCA CCGTGCCAGG GAAACATGAA AAAGACAGTT TAAATGAAGC CTCCAAAAGT 
TCCGTGGCTG TGAACAAGTC TAAGCCAGAG GACTCCAAGA ATCCAGCAAG CATGGAGATC 
ACAGCGGGTG AAAGAAATGT GACCCTACCG GATTCACAAG CACAGGGCAG TTTAGCTGAT 
GGGCTTCCCC TGGAAACAGC ACTACAAGAG CCATTGGAAA GTAGCATCCC TGGGAGTGAT 
GGAAGGGATG GGGTAGATAA TAGATCCATG AGAAGATCCC TTTCCTCCAG CAAACCACAC 
CTAAAACCAG CTCTGGGTAT GAATGGCGCC AAAGCCCGCA GCCACAGCTT CAGTACACAC 
TCAGGAGACA AGCCTTCTAC GCCCCCCATC GAAGGGTCAG GCAAAGTCCG CACTCAGATC 
ATTACCAATA CCGCCGAGAG AGGCAATTCT CTTACCCGGC AGAACTCTTC CACGGAAAGC 
TCTCCCAACA AGGCCCCTTC TGCTCCCATG TTGGAGAGTC TCCCCAGTGT TGGGAGGCCC 
TCGGGGCACC CCTCCTCCGG GAAGGGCTCC CTGGGGAGCT CAGGCAGCTT CAGCAGTCAG 
CATGGGAGCC CAAGTAAGTT GCCTTTGAGG ATCCCTCCAA AGTCTGAGGG ACTCCTCATC 
CCACCTGGAA AGGAAGACCA GCAGGCCTTC ACCCAGGGAG AGTGCCCCAG TGCCAATGTG 
GCTGTACTTG GGGAACCAGG CAGTGACCGC CGCAGTTGCC CACCCACCCC AACAGACTGC 
CCTGAAGCCC TGCAGAGCCC AGGGAGGACT CAGCATCCAA GCACTTTTGA AACAAGCAGT 
ACATCCAAGC TAGAAACTTC TGGAAGGCAT CCAGATGCCT CTGCAACCGC GACTGATGCT 
GTGAGTTCAG AAGCCCCCCT CTCACCCACA ATCGAAGAAA AGGTCATGTT GTGCATTCAG 
GAAAATGTGG AAAAGGGCCA AGTGCAAACA AAGCCCACCT CTGTGGAAGC AAAGCAGAAG 
CCTGGGCCTT CTTTTGCCAG CTGGTTTGGT TTTCGGAAGA GTAGACTTCC AGCTCTGAGT 
AGCAGGAAAA TGGACATCTC CAAAACCAAA GTAGAAAAGA AAGATGCAAA AGTCTTGGGG 
TTTGGAAACA GACAACTAAA ATCAGAAAGA AAAAAAGAGA AAAAGAAGCC TGAACTACAG 
TGTGAGACAG AAAATGAGCT TATCAAGGAC ACCAAGTCAG CAGATAATCC AGATGGCGGT 
TTACAAAGCA AAAATAATCG GAGAACACCA CAAGACATTT ACAACCAACT GAAGATTGAA 
CCAAGGAATA GACACAGCCC TGTTGCATGT TCAACGAAAG ACACCTTCAT GACGGAACTC 
TTGAACAGAG TTGATAAGAA AGCAGCTCCA CAGACAGAAA GTGGATCAAG TAATGCTTCC 
TGCAGGAATG TGTTAAAGGG CAGTTCTCAG GGCTCCTGTC TCATCGGCAG CTCTATCAGT 
ACTCAAGGAA ACCACAAGAA AAACATGAAA ATCAAAGCCG ATATGGAAGT ACCAAAAGAC 
TCCCTGGTAA AAGAGGCAAA TGAAAACTTG CAAGAGGATG AAGACGATGC AGTTGCAGAT 
TCTGTATTTC AGAGCCACAT CATAGAATCC AACTGCCAGA TGAGAACATT GGACAGTGGG 
ATCGGAACCT TTCCACTCCC AGACTCGGGA AATCGCTCGA CAGGACGCTA CCTATGCCAG 
CCAGACTCCC CAGAGGACGC TGAGCCTCTC CTGCCTCTCC AGTCAGCCCT TTCTGCAGTT 
TCTTCCATGA GAGCCCAAAC CCTTGAACGT GAAGTGCCTT CCTCCACAGA CGGCCAGCGC 
CCTGCAGATA GCGCCATTGT TCATTCCACA TCCGACCCCA TCATGACCGC CAGAGGGATG 
AGGCCTCTTC AGAGCCGCCT CCCCAAACCA GCTTCCTCAG GAAAAGTCAG TTCCCAAAAG 
CAGAATGAAG CAGAGCCAAG GCCTCAGACA TGCTCATCAT TCGGATATGC TGAAGACCCA 
ATGGCAAGCC AGCCGCTTCC AGACTGGGGG AGTGAAGTTG CTGCCACCGG GACCCAGAAA 
TTGAGACAAC TTGAAGAAAC AAAAGACGAT CCCGAGAATA GATTATCGAA AATTTCCCTA 
GAGTCATTCA ATAAATTTAA CAGCAATACT GTGATTTTAT TAGAAAAAGA GAAGAACTCT 
CTGAACAAGG TTGAAGGACA GAAGGAAGAA AAAGAAAAAA ATGAAGAGAC ATCTTTGAGT 
AGTTCAGATA GGCCTGGGGT AGACAACTTG GAATCTTTGA GTGATTCTTT ATATGATAGC 
TTCTCTTCCT GTGCCAGTCA AGGTTCAAAT GATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A genome-wide association study of brain lesion distribution in multiple sclerosis.
Brain : a journal of neurology136(Pt 4)1012-24(2013 Apr)
Gourraud PA,Sdika M,Khankhanian P,Henry RG,Beheshtian A,Matthews PM,Hauser SL,Oksenberg JR,Pelletier D,Baranzini SE

Genome-wide association study identifies novel loci associated with concentrations of four plasma phospholipid fatty acids in the de novo lipogenesis pathway: results from the Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) consortium.
Circulation. Cardiovascular genetics6(2)171-83(2013 Apr)
Wu JH,Lemaitre RN,Manichaikul A,Guan W,Tanaka T,Foy M,Kabagambe EK,Djousse L,Siscovick D,Fretts AM,Johnson C,King IB,Psaty BM,McKnight B,Rich SS,Chen YD,Nettleton JA,Tang W,Bandinelli S,Jacobs DR Jr,Browning BL,Laurie CC,Gu X,Tsai MY,Steffen LM,Ferrucci L,Fornage M,Mozaffarian D

Genome-wide association study of HLA-DQB1*06:02 negative essential hypersomnia.
PeerJ1e66(2013)
Khor SS,Miyagawa T,Toyoda H,Yamasaki M,Kawamura Y,Tanii H,Okazaki Y,Sasaki T,Lin L,Faraco J,Rico T,Honda Y,Honda M,Mignot E,Tokunaga K

A genome-wide association study in the Japanese population confirms 9p21 and 14q23 as susceptibility loci for primary open angle glaucoma.
Human molecular genetics21(12)2836-42(2012 Jun)
Osman W,Low SK,Takahashi A,Kubo M,Nakamura Y

A genome-wide meta-analysis identifies novel loci associated with schizophrenia and bipolar disorder.
Schizophrenia research124(1-3)192-9(2010 Dec)
Wang KS,Liu XF,Aragam N

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NCKAP5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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