NCKAP5 cDNA ORF clone, Bos taurus(cattle)

The following NCKAP5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCKAP5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa227745 XM_024980953.1
Latest version!
Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227746 XM_010802114.3
Latest version!
Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227747 XM_015475847.2
Latest version!
Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227748 XM_024980966.1
Latest version!
Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa87935 XM_015475847.1
Latest version!
Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa87935 XM_015462321.1
Latest version!
Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa16499 XM_002685418.4
Latest version!
Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa16499 XM_015462322.1
Latest version!
Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa58058 XM_010802114.2
Latest version!
Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa58058 XM_015462320.1
Latest version!
Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa227745
    Clone ID Related Accession (Same CDS sequence) XM_024980953.1
    Accession Version XM_024980953.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5847bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nck-associated protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGGGCA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TTTAGATAGC 
    AGTCTCGTGG AATACATGGA CTCCAACAAA TACATTGAAC ATCTGTTAAA TCAACTTGAG
    GAGCAACACA GAAGTCTCTG GAGGGAGAAG TTGGCTGTGG CCCGACTGCA GAGAGAAGTT
    GCCCAGAGAA GAAGTGAAGG GGCCATGCAT GAGAAGCTGA TACATGAATT GGAAGAGGAG
    AGACACTTGC GTCTTCAGAG TGAGAAGCGC TTGCAGGAGG TGACACTAGA GTCTGAGCGC
    AACAGAATTC AGATGCGCGG CCTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA
    AATCTACTAC AGAGTCAAGG ACCTCCGGAA CAGAAAAAAG AAGAAACTGT TAATATAATG
    GTCTATCAGG AAAAACTGTC AGAAGAAGAG CGAAAACACA AGGCGGCTTT GGAAGGCCGT
    CACATGGCGC TAGATGAGGA CTCGAGGAGT GAAGGGAGCA GTGCAGATGA GGGGAAAGGC
    AAGACCAAAT GGCTGTTAGA AAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCATTGGCT
    TTGGAAAATG AAAATCAAAG GGAACAGTAT GAGAGATGTC TTGATGAGGT AGCCAACCAG
    GTTGTGCAAG CCCTGCTCAC TCAAAAGGAT CTCAGGGAGG AATGTGTAAA GTTGAAAACA
    AGAGTGTTTG ATTTGGAACA ACAGAATCGA ACGCTAAGCA TCCTTTTCCA ACAGCGAGTC
    AGACCAACTT CTGATCTCCT CCTCCAGAAA CTTCACTCAC GCATCTTAGA TCTTTCATCC
    GGGGATTTAC TCTCAGATGT GGAAGGAAGC CAGAGTCTGA CGCACTCACG AACTGATGTT
    GAAATGCATG GCTGCCAGCT GAACACAAAA GCAGGAACCC CTGCTTTGAA ATGCCCTGGC
    ACGGGGATTG CCGTCTCTGG TCATCTCTGC CCTCGAAGCA GCTACAGCAG CAGTGAGCTG
    TCTCTTTCCA GCACCTGCAG CGAGTATTCC AGCGGCTCCT CCTACACGTG GCATGATGGA
    AAGAACCTCA GGAAAAGGCA GTCATCACAA AATTGGGACA AAAGGTTAAG TATTGACTCT
    TCGCTCCCGA GTGGCTTTGC TAGTCCTATG GATGAACTAC CTCCAACTCG CATCAAGGAA
    AGCCACATTT TGGAAGGACT AAGAAAGCTC CAGAAGCGAA AAGTGTTACT TGAACCCCCA
    TCAGTGATAA CCAAATGGGG TTATAAAGAT TGCATGAACT CCAATGAAGG AATCTATTCT
    CCAGGAATTA ATAGTAACAG CCTGAAGGAA TATCCACCCT GCAAACCCAC AGACACAAGG
    AGCCCCTGCA CGGATCCCCA CAAGGCATTT GTTTATGACA CGGATTCTCA TGACGAGGCT
    GATGAGGACG CTTCCTCCTT AGCCTCTGTC CAAGCGGTTC CAAACCAGGG CTGCAGGCTG
    CACAGTTGTA AATTAACCCA CAGTGTCTCT GACAGCCTCT TTGGCTGGGA GCTGAATGGC
    AAACACTGTT CAGAAGTCAC TTCCTCGGTC TACTCCAGGG AGAGGCCTGA GAAGCTGACC
    GGTTGTGCCA GCAACTGTCC CTTGGAGCAG AAGCTGTGCC CAGGCATGCA GGGGCCTCGG
    GTACAGAGAG AGAAGGTCTC ACCCAGCCAG GGCTGCCAGG CCCTCAGTCT CCAGCCCTCA
    GACACCGATG ACCCGGAGAC CCTGGATGAG CTGCACATCG AGAGCAGTGA TGAGAAAAGC
    CCTTCAGATG TGTCTGCAAC CGCGGACACT GACCAGTCCA CGGAGAGCCT GGATATCCTC
    ACAGGATTTG AAAAATCTTC ATGTGGGTCT CCCGAGGAGG AAGAAAACCA AGTACCCGTC
    CATTTAGAGA GCAGGCCAAA GACGTTTAGT TTCATTAAGC AACAGAGGGT TGTAAAACGG
    ACTTCCTCAG AAGAATGTAT TACTGTGATA TTTGATGCAG AAGACGGCGA GCCCATTGAG
    TTCAGCTCTC ACCAGACTGG CCTTGTTACT GTTACCAGAA AGGAGATTTC CATCCATCAG
    GCCCCTGCTG GGCCCCAGAT GGAACACACT GAACTTTCAC CTCAGGGAAT TGCTCATTTA
    CAGCCAGGGG CTGCAGCTAG AGACTACCCT TTTTTAAAGA GGTCTGAAGA GGAAACTGAA
    AGAAACATTC CACGAGATGA GGTAGATGAT CCTGCCATGA CACCTGCTGT GTCTTTCCAC
    CCCAGGACAG TGACGCAAAA TACTCAAAGA CTGGCCAAAC CGACACATGT CACACCATGC
    CAGAGTCATT CTCGGTCATC TGTGGGCACA GGCGTCTGTC AGAAGAAAAG TCTGACAAAA
    ATACCTACCA GGGGCAAGTC TTCACCTCAG AAATCGAGAG TAAGGGAGCC TGAAGCCTCC
    CTTATGATGC CCTCAGCTGG CTCAGTGACT CTTGAGAAAT CACCAGCTCC AGGGAAACTC
    TCACAGTTCA AAAAGACTGA AGGTCCAGCT CCCCTTTTTG ATTTGCAGCC AGATTCACAC
    ATTCCAAAAC CCCCCACCCA GCTTCCTCAT GGCTCCAAGA TGTCCAGCAG AAGGGATTGG
    GTCCAGAGCT CCAAGAATCA GATGCTAGCA TCACAGTTGC TGTCCAGGCC CCCCACTGAG
    CGGAGTGACG ATGGGGAGCC CCCCACGAGG GATAAACACT GTGATCCAGG GCCAGAGGCC
    GGGGTGAAAT CGCCCTCCCC ACCATCCCCT CCAGGCAGGT CGGTCTCCCT ACTCATCAGG
    CCCAGTTATG ACTTTTTGCC ACCACCTTCA TCTGCCAAAT CCGAAAGCAG GGTCTCCACC
    GAGACAGCAA GAATGGTATT GAAATCACCC CCTCTGAAAG GATCCTCTGC TCCTGTTATT
    TATTTTAACC AGACCCTGAC AGATGTGCAG GGGAAGAAAC CGTCTGTGGC CTTCAAAAAG
    CCCATCTTCA CTCCCTCACC ACCCTCCGCA GAAACAGCAA TCCAGATGAG ATGCCCTGCT
    CACAGCCCCT CCAGCTCATT TGCTGTGATG GTCCCGGAGC CCCCAAAAGT CTCTCCAAAG
    AGAAATGCCC CCAGAGCCCC ACCTCACCAA ACACTTGGGA CCACACAGAA CAACACAGGG
    TTACAGACTC CCAAGAACTA TGCTTCCCCC CGTGAGCCAC TGGAAATACC ATCCTCCAAA
    GGTGTATCTC CAGGGAGAAA AGAAAAGTTG AGTGGCAGTG TCTCTGCATC TTCCAAGCCT
    TCCTTCTTAG GGGTAAATGA GTCACCATCA TCTCAGGTTA ACAGTCCCTT ATCATCAGCA
    TCCTTCAAAA GCCATAACCC CCTACATGGT TGTCAAAACC TTCATGAGAG AGGACTGAAA
    ACTCGCTTCC CAGTTGGGCT CAAAGTTTTC ATGAAGTCTC CCCAGTTGCT CAGGAAAAGT
    TCCACGGTGC CAGGGAAACA GGAAAAAGAC AGTCTAAATG AAGCCTCGAA AACCTCTGTG
    GCTGTGAGCA AGGCTAAGCC AGGGGCCTCC AGAAATCCAG CCAGCCTTGA GACCACAGGA
    GGGGAAAGAA ATACGTCTCC AGTGAGTTTA CCAGCACAAG AGAGTTTGGC CAAAGGGCTT
    CCCCTGGAAA GAGCAATGCC AGAGTCACTG GAAAATAGCA TGCCTGGGGC TGATAGAAAA
    GATGGAGTAG AAAACAGGTC TGTAAAAAGA TCCCTGTCCT CCAGCAAACC ACACCTGAAA
    CCAGCTCTAG GCATGAACGG AGCCAAAGCC CGCAGCCAGA GTTTCAGTGC TCACTCAGGG
    GAAAAGCCCC CCACGCCCCC CACAGAAGGG CTGGGCAAAG TCCGGACTCA AATTATTACC
    AACACCGCTG AGAGAGGCAA TTCCCTCACC CGGCAGAGCT CTTCTGCCGA AGGCTCTCCC
    AGCAAGAGTG CGTCTACCCC TGTGTCTGAT GGTCTCCCCA GCACTGGGAA GCCCCTGGGA
    CACCCTTCCC CCAGGCAGGG TTCCTTGGGG AGCACAGGCA GCTCCAGCAG CCAGCATGGG
    AGCCCAAGCA AGTTGCCTTT GAGGATCCCT CCAAAATCCG AGGAGCCCCT CACCCCAGCT
    GGAACAGAAG AGCAGCAGGC CTATGCCCAG GGAGAAGGCC CCAGGGTGAC GGTACCTGAG
    GAACCAGGCA GCGACCACTG CAGGTGCCCA CCTACCCCAA CAGATAGCTC AGGAGGCCCA
    CAGAGCCTGG GGAGGACGCC ACATCCCAGC AGTTTCACAG CAAGCAGGAC CTCCAAGCTA
    GAAACTTCCG GAAGGTATCC AGATACTTCT ACAACCAGGG CCGGTGTCGT AAGCCCAGAA
    GCCCCGCTCT CACCCACAAT TGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA
    AAGGGCCAAG TGCAAACAAA GTCCACCTCT GTGGAAGCAA AACCGAAGCC GGGGCCTTCT
    TTTGCCAGCT GGTTCGGTTT TCGGAGGAGC AGACTCCCGG CTCTCAGTAG CAGGAAGATG
    GATGTCTCCA GAACCAAAGC AGAAAAGAAA GATGCAAAAG GTTTAGGGTT TGGAAACAAA
    CAGCTGAAAT CAGAGAGAAA GAAAGAGAAA AAGAAGCCTG AGCTACAGTG TGAGATGGAA
    AATGAACTTC ACAGGGATAT CGAGTTGGCA GATGGTCCAG ACAGTGGTTT GCAAAACAGA
    AATAACCCGA AAACACCGCC AGACATTTAT GATCAAGTGA AGTTTGAATC AAGAAATAGA
    CCCAGCCCTG TTCCATGTTC AGCGAAAGAT ACCTTTATGA CAGAACTTCT GAACAGAGTT
    GATAAGAAAG CAGCTCAACA GACAGAAAGT GGATCAAATA GTCTCTCCTG TAGGAGTGTG
    TTAAAGGGCA GCTCCCAGGG CTCCTGTCTC ACCAGCAGCT CTCTCAGCAC TCAAGGAAAC
    CACAAGAAAA ACATCAAAAC CAAAGCAGAT ATGGAAAAAC CGAAAGGCTC CCTGGTAAGA
    GAGGCAAATG ATCACCTGCA AGAGGATGAA GAAGATACAG TCGCAGATTC TGCATTTCAG
    AGCCATACCA TAGAAACAAA CTGCCAGATG AGGACCCTGG ACAGTGGGAT TGGAACCTTC
    CCACTCCCAG ACTCAGGAAC TCGTGCAGCG GGACGATACA TACGCCAAGC AGACTCCCCT
    GAGGACACTG ACCCCATCCT GTCTCTCCAA CCGGCCCTTT GTGCAGCCTC TTCCATCAGA
    GCCCAGACCC TTGAAAGAGA AGTGCCTTCC TCTGCAGATA GCCAGGGCTC TGCAGACAAT
    GCCATTGTTC ACTCCACATC CGACCCCATC ATGACTGCCA GAGGGACGCG ACCTCTTCAG
    AGTCTCCTCC CCAAACCAGC CTCCTCAGGA AAAATCAATT CCCAAATGAA GAGTGAAGCC
    GAGCCAAGGT CTCAAAATTG TTCACCATTC CAATATGTCG AAAACACAAT GGCGAGCAAG
    CCACTTCCAG CCTGGGAGGG TGATGACGCT GCTGCAGAGA CCCAGAAATT GAAACAAGTT
    GAAGAAACCA GAGAAGATCC TGAGAATAGA TTATGCAAAA TTTCCCTGGA GTCATTCAAT
    AAATATGATG GCAATACTGT GATCTTACTA GGAAAGGAGA AGAACTCTCT GAACAAGGTT
    GAAGGACAAA AGGAAGAAAA AGAAAAAACT GAAGAGGCCT CTTTGAGTAG CTCTGAGAGA
    CCTGGGGTAG ACCACCTGGA ATCTTTGAGT GATTCTTTAT ATGATAGCTT CTCTTCCTGT
    GCAAGTCAGG GTTCAAATGA TGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836721.1
    CDS623..6469
    Translation

    Target ORF information:

    RefSeq Version XM_024980953.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980953.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGGGCA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TTTAGATAGC 
    AGTCTCGTGG AATACATGGA CTCCAACAAA TACATTGAAC ATCTGTTAAA TCAACTTGAG
    GAGCAACACA GAAGTCTCTG GAGGGAGAAG TTGGCTGTGG CCCGACTGCA GAGAGAAGTT
    GCCCAGAGAA GAAGTGAAGG GGCCATGCAT GAGAAGCTGA TACATGAATT GGAAGAGGAG
    AGACACTTGC GTCTTCAGAG TGAGAAGCGC TTGCAGGAGG TGACACTAGA GTCTGAGCGC
    AACAGAATTC AGATGCGCGG CCTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA
    AATCTACTAC AGAGTCAAGG ACCTCCGGAA CAGAAAAAAG AAGAAACTGT TAATATAATG
    GTCTATCAGG AAAAACTGTC AGAAGAAGAG CGAAAACACA AGGCGGCTTT GGAAGGCCGT
    CACATGGCGC TAGATGAGGA CTCGAGGAGT GAAGGGAGCA GTGCAGATGA GGGGAAAGGC
    AAGACCAAAT GGCTGTTAGA AAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCATTGGCT
    TTGGAAAATG AAAATCAAAG GGAACAGTAT GAGAGATGTC TTGATGAGGT AGCCAACCAG
    GTTGTGCAAG CCCTGCTCAC TCAAAAGGAT CTCAGGGAGG AATGTGTAAA GTTGAAAACA
    AGAGTGTTTG ATTTGGAACA ACAGAATCGA ACGCTAAGCA TCCTTTTCCA ACAGCGAGTC
    AGACCAACTT CTGATCTCCT CCTCCAGAAA CTTCACTCAC GCATCTTAGA TCTTTCATCC
    GGGGATTTAC TCTCAGATGT GGAAGGAAGC CAGAGTCTGA CGCACTCACG AACTGATGTT
    GAAATGCATG GCTGCCAGCT GAACACAAAA GCAGGAACCC CTGCTTTGAA ATGCCCTGGC
    ACGGGGATTG CCGTCTCTGG TCATCTCTGC CCTCGAAGCA GCTACAGCAG CAGTGAGCTG
    TCTCTTTCCA GCACCTGCAG CGAGTATTCC AGCGGCTCCT CCTACACGTG GCATGATGGA
    AAGAACCTCA GGAAAAGGCA GTCATCACAA AATTGGGACA AAAGGTTAAG TATTGACTCT
    TCGCTCCCGA GTGGCTTTGC TAGTCCTATG GATGAACTAC CTCCAACTCG CATCAAGGAA
    AGCCACATTT TGGAAGGACT AAGAAAGCTC CAGAAGCGAA AAGTGTTACT TGAACCCCCA
    TCAGTGATAA CCAAATGGGG TTATAAAGAT TGCATGAACT CCAATGAAGG AATCTATTCT
    CCAGGAATTA ATAGTAACAG CCTGAAGGAA TATCCACCCT GCAAACCCAC AGACACAAGG
    AGCCCCTGCA CGGATCCCCA CAAGGCATTT GTTTATGACA CGGATTCTCA TGACGAGGCT
    GATGAGGACG CTTCCTCCTT AGCCTCTGTC CAAGCGGTTC CAAACCAGGG CTGCAGGCTG
    CACAGTTGTA AATTAACCCA CAGTGTCTCT GACAGCCTCT TTGGCTGGGA GCTGAATGGC
    AAACACTGTT CAGAAGTCAC TTCCTCGGTC TACTCCAGGG AGAGGCCTGA GAAGCTGACC
    GGTTGTGCCA GCAACTGTCC CTTGGAGCAG AAGCTGTGCC CAGGCATGCA GGGGCCTCGG
    GTACAGAGAG AGAAGGTCTC ACCCAGCCAG GGCTGCCAGG CCCTCAGTCT CCAGCCCTCA
    GACACCGATG ACCCGGAGAC CCTGGATGAG CTGCACATCG AGAGCAGTGA TGAGAAAAGC
    CCTTCAGATG TGTCTGCAAC CGCGGACACT GACCAGTCCA CGGAGAGCCT GGATATCCTC
    ACAGGATTTG AAAAATCTTC ATGTGGGTCT CCCGAGGAGG AAGAAAACCA AGTACCCGTC
    CATTTAGAGA GCAGGCCAAA GACGTTTAGT TTCATTAAGC AACAGAGGGT TGTAAAACGG
    ACTTCCTCAG AAGAATGTAT TACTGTGATA TTTGATGCAG AAGACGGCGA GCCCATTGAG
    TTCAGCTCTC ACCAGACTGG CCTTGTTACT GTTACCAGAA AGGAGATTTC CATCCATCAG
    GCCCCTGCTG GGCCCCAGAT GGAACACACT GAACTTTCAC CTCAGGGAAT TGCTCATTTA
    CAGCCAGGGG CTGCAGCTAG AGACTACCCT TTTTTAAAGA GGTCTGAAGA GGAAACTGAA
    AGAAACATTC CACGAGATGA GGTAGATGAT CCTGCCATGA CACCTGCTGT GTCTTTCCAC
    CCCAGGACAG TGACGCAAAA TACTCAAAGA CTGGCCAAAC CGACACATGT CACACCATGC
    CAGAGTCATT CTCGGTCATC TGTGGGCACA GGCGTCTGTC AGAAGAAAAG TCTGACAAAA
    ATACCTACCA GGGGCAAGTC TTCACCTCAG AAATCGAGAG TAAGGGAGCC TGAAGCCTCC
    CTTATGATGC CCTCAGCTGG CTCAGTGACT CTTGAGAAAT CACCAGCTCC AGGGAAACTC
    TCACAGTTCA AAAAGACTGA AGGTCCAGCT CCCCTTTTTG ATTTGCAGCC AGATTCACAC
    ATTCCAAAAC CCCCCACCCA GCTTCCTCAT GGCTCCAAGA TGTCCAGCAG AAGGGATTGG
    GTCCAGAGCT CCAAGAATCA GATGCTAGCA TCACAGTTGC TGTCCAGGCC CCCCACTGAG
    CGGAGTGACG ATGGGGAGCC CCCCACGAGG GATAAACACT GTGATCCAGG GCCAGAGGCC
    GGGGTGAAAT CGCCCTCCCC ACCATCCCCT CCAGGCAGGT CGGTCTCCCT ACTCATCAGG
    CCCAGTTATG ACTTTTTGCC ACCACCTTCA TCTGCCAAAT CCGAAAGCAG GGTCTCCACC
    GAGACAGCAA GAATGGTATT GAAATCACCC CCTCTGAAAG GATCCTCTGC TCCTGTTATT
    TATTTTAACC AGACCCTGAC AGATGTGCAG GGGAAGAAAC CGTCTGTGGC CTTCAAAAAG
    CCCATCTTCA CTCCCTCACC ACCCTCCGCA GAAACAGCAA TCCAGATGAG ATGCCCTGCT
    CACAGCCCCT CCAGCTCATT TGCTGTGATG GTCCCGGAGC CCCCAAAAGT CTCTCCAAAG
    AGAAATGCCC CCAGAGCCCC ACCTCACCAA ACACTTGGGA CCACACAGAA CAACACAGGG
    TTACAGACTC CCAAGAACTA TGCTTCCCCC CGTGAGCCAC TGGAAATACC ATCCTCCAAA
    GGTGTATCTC CAGGGAGAAA AGAAAAGTTG AGTGGCAGTG TCTCTGCATC TTCCAAGCCT
    TCCTTCTTAG GGGTAAATGA GTCACCATCA TCTCAGGTTA ACAGTCCCTT ATCATCAGCA
    TCCTTCAAAA GCCATAACCC CCTACATGGT TGTCAAAACC TTCATGAGAG AGGACTGAAA
    ACTCGCTTCC CAGTTGGGCT CAAAGTTTTC ATGAAGTCTC CCCAGTTGCT CAGGAAAAGT
    TCCACGGTGC CAGGGAAACA GGAAAAAGAC AGTCTAAATG AAGCCTCGAA AACCTCTGTG
    GCTGTGAGCA AGGCTAAGCC AGGGGCCTCC AGAAATCCAG CCAGCCTTGA GACCACAGGA
    GGGGAAAGAA ATACGTCTCC AGTGAGTTTA CCAGCACAAG AGAGTTTGGC CAAAGGGCTT
    CCCCTGGAAA GAGCAATGCC AGAGTCACTG GAAAATAGCA TGCCTGGGGC TGATAGAAAA
    GATGGAGTAG AAAACAGGTC TGTAAAAAGA TCCCTGTCCT CCAGCAAACC ACACCTGAAA
    CCAGCTCTAG GCATGAACGG AGCCAAAGCC CGCAGCCAGA GTTTCAGTGC TCACTCAGGG
    GAAAAGCCCC CCACGCCCCC CACAGAAGGG CTGGGCAAAG TCCGGACTCA AATTATTACC
    AACACCGCTG AGAGAGGCAA TTCCCTCACC CGGCAGAGCT CTTCTGCCGA AGGCTCTCCC
    AGCAAGAGTG CGTCTACCCC TGTGTCTGAT GGTCTCCCCA GCACTGGGAA GCCCCTGGGA
    CACCCTTCCC CCAGGCAGGG TTCCTTGGGG AGCACAGGCA GCTCCAGCAG CCAGCATGGG
    AGCCCAAGCA AGTTGCCTTT GAGGATCCCT CCAAAATCCG AGGAGCCCCT CACCCCAGCT
    GGAACAGAAG AGCAGCAGGC CTATGCCCAG GGAGAAGGCC CCAGGGTGAC GGTACCTGAG
    GAACCAGGCA GCGACCACTG CAGGTGCCCA CCTACCCCAA CAGATAGCTC AGGAGGCCCA
    CAGAGCCTGG GGAGGACGCC ACATCCCAGC AGTTTCACAG CAAGCAGGAC CTCCAAGCTA
    GAAACTTCCG GAAGGTATCC AGATACTTCT ACAACCAGGG CCGGTGTCGT AAGCCCAGAA
    GCCCCGCTCT CACCCACAAT TGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA
    AAGGGCCAAG TGCAAACAAA GTCCACCTCT GTGGAAGCAA AACCGAAGCC GGGGCCTTCT
    TTTGCCAGCT GGTTCGGTTT TCGGAGGAGC AGACTCCCGG CTCTCAGTAG CAGGAAGATG
    GATGTCTCCA GAACCAAAGC AGAAAAGAAA GATGCAAAAG GTTTAGGGTT TGGAAACAAA
    CAGCTGAAAT CAGAGAGAAA GAAAGAGAAA AAGAAGCCTG AGCTACAGTG TGAGATGGAA
    AATGAACTTC ACAGGGATAT CGAGTTGGCA GATGGTCCAG ACAGTGGTTT GCAAAACAGA
    AATAACCCGA AAACACCGCC AGACATTTAT GATCAAGTGA AGTTTGAATC AAGAAATAGA
    CCCAGCCCTG TTCCATGTTC AGCGAAAGAT ACCTTTATGA CAGAACTTCT GAACAGAGTT
    GATAAGAAAG CAGCTCAACA GACAGAAAGT GGATCAAATA GTCTCTCCTG TAGGAGTGTG
    TTAAAGGGCA GCTCCCAGGG CTCCTGTCTC ACCAGCAGCT CTCTCAGCAC TCAAGGAAAC
    CACAAGAAAA ACATCAAAAC CAAAGCAGAT ATGGAAAAAC CGAAAGGCTC CCTGGTAAGA
    GAGGCAAATG ATCACCTGCA AGAGGATGAA GAAGATACAG TCGCAGATTC TGCATTTCAG
    AGCCATACCA TAGAAACAAA CTGCCAGATG AGGACCCTGG ACAGTGGGAT TGGAACCTTC
    CCACTCCCAG ACTCAGGAAC TCGTGCAGCG GGACGATACA TACGCCAAGC AGACTCCCCT
    GAGGACACTG ACCCCATCCT GTCTCTCCAA CCGGCCCTTT GTGCAGCCTC TTCCATCAGA
    GCCCAGACCC TTGAAAGAGA AGTGCCTTCC TCTGCAGATA GCCAGGGCTC TGCAGACAAT
    GCCATTGTTC ACTCCACATC CGACCCCATC ATGACTGCCA GAGGGACGCG ACCTCTTCAG
    AGTCTCCTCC CCAAACCAGC CTCCTCAGGA AAAATCAATT CCCAAATGAA GAGTGAAGCC
    GAGCCAAGGT CTCAAAATTG TTCACCATTC CAATATGTCG AAAACACAAT GGCGAGCAAG
    CCACTTCCAG CCTGGGAGGG TGATGACGCT GCTGCAGAGA CCCAGAAATT GAAACAAGTT
    GAAGAAACCA GAGAAGATCC TGAGAATAGA TTATGCAAAA TTTCCCTGGA GTCATTCAAT
    AAATATGATG GCAATACTGT GATCTTACTA GGAAAGGAGA AGAACTCTCT GAACAAGGTT
    GAAGGACAAA AGGAAGAAAA AGAAAAAACT GAAGAGGCCT CTTTGAGTAG CTCTGAGAGA
    CCTGGGGTAG ACCACCTGGA ATCTTTGAGT GATTCTTTAT ATGATAGCTT CTCTTCCTGT
    GCAAGTCAGG GTTCAAATGA TGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227746
    Clone ID Related Accession (Same CDS sequence) XM_010802114.3
    Accession Version XM_010802114.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5844bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nck-associated protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802114.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGGGCA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TTTAGATAGC 
    AGTCTCGTGG AATACATGGA CTCCAACAAA TACATTGAAC ATCTGTTAAA TCAACTTGAG
    GAGCAACACA GAAGTCTCTG GAGGGAGAAG TTGGCTGTGG CCCGACTGCA GAGAGAAGTT
    GCCCAGAGAA GAAGTGAAGG GGCCATGCAT GAGAAGCTGA TACATGAATT GGAAGAGGAG
    AGACACTTGC GTCTTCAGAG TGAGAAGCGC TTGCAGGAGG TGACACTAGA GTCTGAGCGC
    AACAGAATTC AGATGCGCGG CCTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA
    AATCTACTAC AGAGTCAAGG ACCTCCGGAA CAGAAAAAAG AAGAAACTGT TAATATAATG
    GTCTATCAGG AAAAACTGTC AGAAGAAGAG CGAAAACACA AGGCGGCTTT GGAAGGCCGT
    CACATGGCGC TAGATGAGGA CTCGAGGAGT GAAGGGAGCA GTGCAGATGA GGGGAAAGGC
    AAGACCAAAT GGCTGTTAGA AAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCATTGGCT
    TTGGAAAATG AAAATCAAAG GGAACAGTAT GAGAGATGTC TTGATGAGGT AGCCAACCAG
    GTTGTGCAAG CCCTGCTCAC TCAAAAGGAT CTCAGGGAGG AATGTGTAAA GTTGAAAACA
    AGAGTGTTTG ATTTGGAACA ACAGAATCGA ACGCTAAGCA TCCTTTTCCA ACAGCGAGTC
    AGACCAACTT CTGATCTCCT CCTCCAGAAA CTTCACTCAC GCATCTTAGA TCTTTCATCC
    GGGGATTTAC TCTCAGATGT GGAAGGAAGC CAGAGTCTGA CGCACTCACG AACTGATGTT
    GAAATGCATG GCTGCCAGCT GAACACAAAA GCAGGAACCC CTGCTTTGAA ATGCCCTGGC
    ACGGGGATTG CCGTCTCTGG TCATCTCTGC CCTCGAAGCA GCTACAGCAG CAGTGAGCTG
    TCTCTTTCCA GCACCTGCAG CGAGTATTCC AGCGGCTCCT CCTACACGTG GCATGATGGA
    AAGAACCTCA GGAAAAGGTC ATCACAAAAT TGGGACAAAA GGTTAAGTAT TGACTCTTCG
    CTCCCGAGTG GCTTTGCTAG TCCTATGGAT GAACTACCTC CAACTCGCAT CAAGGAAAGC
    CACATTTTGG AAGGACTAAG AAAGCTCCAG AAGCGAAAAG TGTTACTTGA ACCCCCATCA
    GTGATAACCA AATGGGGTTA TAAAGATTGC ATGAACTCCA ATGAAGGAAT CTATTCTCCA
    GGAATTAATA GTAACAGCCT GAAGGAATAT CCACCCTGCA AACCCACAGA CACAAGGAGC
    CCCTGCACGG ATCCCCACAA GGCATTTGTT TATGACACGG ATTCTCATGA CGAGGCTGAT
    GAGGACGCTT CCTCCTTAGC CTCTGTCCAA GCGGTTCCAA ACCAGGGCTG CAGGCTGCAC
    AGTTGTAAAT TAACCCACAG TGTCTCTGAC AGCCTCTTTG GCTGGGAGCT GAATGGCAAA
    CACTGTTCAG AAGTCACTTC CTCGGTCTAC TCCAGGGAGA GGCCTGAGAA GCTGACCGGT
    TGTGCCAGCA ACTGTCCCTT GGAGCAGAAG CTGTGCCCAG GCATGCAGGG GCCTCGGGTA
    CAGAGAGAGA AGGTCTCACC CAGCCAGGGC TGCCAGGCCC TCAGTCTCCA GCCCTCAGAC
    ACCGATGACC CGGAGACCCT GGATGAGCTG CACATCGAGA GCAGTGATGA GAAAAGCCCT
    TCAGATGTGT CTGCAACCGC GGACACTGAC CAGTCCACGG AGAGCCTGGA TATCCTCACA
    GGATTTGAAA AATCTTCATG TGGGTCTCCC GAGGAGGAAG AAAACCAAGT ACCCGTCCAT
    TTAGAGAGCA GGCCAAAGAC GTTTAGTTTC ATTAAGCAAC AGAGGGTTGT AAAACGGACT
    TCCTCAGAAG AATGTATTAC TGTGATATTT GATGCAGAAG ACGGCGAGCC CATTGAGTTC
    AGCTCTCACC AGACTGGCCT TGTTACTGTT ACCAGAAAGG AGATTTCCAT CCATCAGGCC
    CCTGCTGGGC CCCAGATGGA ACACACTGAA CTTTCACCTC AGGGAATTGC TCATTTACAG
    CCAGGGGCTG CAGCTAGAGA CTACCCTTTT TTAAAGAGGT CTGAAGAGGA AACTGAAAGA
    AACATTCCAC GAGATGAGGT AGATGATCCT GCCATGACAC CTGCTGTGTC TTTCCACCCC
    AGGACAGTGA CGCAAAATAC TCAAAGACTG GCCAAACCGA CACATGTCAC ACCATGCCAG
    AGTCATTCTC GGTCATCTGT GGGCACAGGC GTCTGTCAGA AGAAAAGTCT GACAAAAATA
    CCTACCAGGG GCAAGTCTTC ACCTCAGAAA TCGAGAGTAA GGGAGCCTGA AGCCTCCCTT
    ATGATGCCCT CAGCTGGCTC AGTGACTCTT GAGAAATCAC CAGCTCCAGG GAAACTCTCA
    CAGTTCAAAA AGACTGAAGG TCCAGCTCCC CTTTTTGATT TGCAGCCAGA TTCACACATT
    CCAAAACCCC CCACCCAGCT TCCTCATGGC TCCAAGATGT CCAGCAGAAG GGATTGGGTC
    CAGAGCTCCA AGAATCAGAT GCTAGCATCA CAGTTGCTGT CCAGGCCCCC CACTGAGCGG
    AGTGACGATG GGGAGCCCCC CACGAGGGAT AAACACTGTG ATCCAGGGCC AGAGGCCGGG
    GTGAAATCGC CCTCCCCACC ATCCCCTCCA GGCAGGTCGG TCTCCCTACT CATCAGGCCC
    AGTTATGACT TTTTGCCACC ACCTTCATCT GCCAAATCCG AAAGCAGGGT CTCCACCGAG
    ACAGCAAGAA TGGTATTGAA ATCACCCCCT CTGAAAGGAT CCTCTGCTCC TGTTATTTAT
    TTTAACCAGA CCCTGACAGA TGTGCAGGGG AAGAAACCGT CTGTGGCCTT CAAAAAGCCC
    ATCTTCACTC CCTCACCACC CTCCGCAGAA ACAGCAATCC AGATGAGATG CCCTGCTCAC
    AGCCCCTCCA GCTCATTTGC TGTGATGGTC CCGGAGCCCC CAAAAGTCTC TCCAAAGAGA
    AATGCCCCCA GAGCCCCACC TCACCAAACA CTTGGGACCA CACAGAACAA CACAGGGTTA
    CAGACTCCCA AGAACTATGC TTCCCCCCGT GAGCCACTGG AAATACCATC CTCCAAAGGT
    GTATCTCCAG GGAGAAAAGA AAAGTTGAGT GGCAGTGTCT CTGCATCTTC CAAGCCTTCC
    TTCTTAGGGG TAAATGAGTC ACCATCATCT CAGGTTAACA GTCCCTTATC ATCAGCATCC
    TTCAAAAGCC ATAACCCCCT ACATGGTTGT CAAAACCTTC ATGAGAGAGG ACTGAAAACT
    CGCTTCCCAG TTGGGCTCAA AGTTTTCATG AAGTCTCCCC AGTTGCTCAG GAAAAGTTCC
    ACGGTGCCAG GGAAACAGGA AAAAGACAGT CTAAATGAAG CCTCGAAAAC CTCTGTGGCT
    GTGAGCAAGG CTAAGCCAGG GGCCTCCAGA AATCCAGCCA GCCTTGAGAC CACAGGAGGG
    GAAAGAAATA CGTCTCCAGT GAGTTTACCA GCACAAGAGA GTTTGGCCAA AGGGCTTCCC
    CTGGAAAGAG CAATGCCAGA GTCACTGGAA AATAGCATGC CTGGGGCTGA TAGAAAAGAT
    GGAGTAGAAA ACAGGTCTGT AAAAAGATCC CTGTCCTCCA GCAAACCACA CCTGAAACCA
    GCTCTAGGCA TGAACGGAGC CAAAGCCCGC AGCCAGAGTT TCAGTGCTCA CTCAGGGGAA
    AAGCCCCCCA CGCCCCCCAC AGAAGGGCTG GGCAAAGTCC GGACTCAAAT TATTACCAAC
    ACCGCTGAGA GAGGCAATTC CCTCACCCGG CAGAGCTCTT CTGCCGAAGG CTCTCCCAGC
    AAGAGTGCGT CTACCCCTGT GTCTGATGGT CTCCCCAGCA CTGGGAAGCC CCTGGGACAC
    CCTTCCCCCA GGCAGGGTTC CTTGGGGAGC ACAGGCAGCT CCAGCAGCCA GCATGGGAGC
    CCAAGCAAGT TGCCTTTGAG GATCCCTCCA AAATCCGAGG AGCCCCTCAC CCCAGCTGGA
    ACAGAAGAGC AGCAGGCCTA TGCCCAGGGA GAAGGCCCCA GGGTGACGGT ACCTGAGGAA
    CCAGGCAGCG ACCACTGCAG GTGCCCACCT ACCCCAACAG ATAGCTCAGG AGGCCCACAG
    AGCCTGGGGA GGACGCCACA TCCCAGCAGT TTCACAGCAA GCAGGACCTC CAAGCTAGAA
    ACTTCCGGAA GGTATCCAGA TACTTCTACA ACCAGGGCCG GTGTCGTAAG CCCAGAAGCC
    CCGCTCTCAC CCACAATTGA AGAAAAGGTC ATGTTGTGCA TTCAGGAAAA TGTGGAAAAG
    GGCCAAGTGC AAACAAAGTC CACCTCTGTG GAAGCAAAAC CGAAGCCGGG GCCTTCTTTT
    GCCAGCTGGT TCGGTTTTCG GAGGAGCAGA CTCCCGGCTC TCAGTAGCAG GAAGATGGAT
    GTCTCCAGAA CCAAAGCAGA AAAGAAAGAT GCAAAAGGTT TAGGGTTTGG AAACAAACAG
    CTGAAATCAG AGAGAAAGAA AGAGAAAAAG AAGCCTGAGC TACAGTGTGA GATGGAAAAT
    GAACTTCACA GGGATATCGA GTTGGCAGAT GGTCCAGACA GTGGTTTGCA AAACAGAAAT
    AACCCGAAAA CACCGCCAGA CATTTATGAT CAAGTGAAGT TTGAATCAAG AAATAGACCC
    AGCCCTGTTC CATGTTCAGC GAAAGATACC TTTATGACAG AACTTCTGAA CAGAGTTGAT
    AAGAAAGCAG CTCAACAGAC AGAAAGTGGA TCAAATAGTC TCTCCTGTAG GAGTGTGTTA
    AAGGGCAGCT CCCAGGGCTC CTGTCTCACC AGCAGCTCTC TCAGCACTCA AGGAAACCAC
    AAGAAAAACA TCAAAACCAA AGCAGATATG GAAAAACCGA AAGGCTCCCT GGTAAGAGAG
    GCAAATGATC ACCTGCAAGA GGATGAAGAA GATACAGTCG CAGATTCTGC ATTTCAGAGC
    CATACCATAG AAACAAACTG CCAGATGAGG ACCCTGGACA GTGGGATTGG AACCTTCCCA
    CTCCCAGACT CAGGAACTCG TGCAGCGGGA CGATACATAC GCCAAGCAGA CTCCCCTGAG
    GACACTGACC CCATCCTGTC TCTCCAACCG GCCCTTTGTG CAGCCTCTTC CATCAGAGCC
    CAGACCCTTG AAAGAGAAGT GCCTTCCTCT GCAGATAGCC AGGGCTCTGC AGACAATGCC
    ATTGTTCACT CCACATCCGA CCCCATCATG ACTGCCAGAG GGACGCGACC TCTTCAGAGT
    CTCCTCCCCA AACCAGCCTC CTCAGGAAAA ATCAATTCCC AAATGAAGAG TGAAGCCGAG
    CCAAGGTCTC AAAATTGTTC ACCATTCCAA TATGTCGAAA ACACAATGGC GAGCAAGCCA
    CTTCCAGCCT GGGAGGGTGA TGACGCTGCT GCAGAGACCC AGAAATTGAA ACAAGTTGAA
    GAAACCAGAG AAGATCCTGA GAATAGATTA TGCAAAATTT CCCTGGAGTC ATTCAATAAA
    TATGATGGCA ATACTGTGAT CTTACTAGGA AAGGAGAAGA ACTCTCTGAA CAAGGTTGAA
    GGACAAAAGG AAGAAAAAGA AAAAACTGAA GAGGCCTCTT TGAGTAGCTC TGAGAGACCT
    GGGGTAGACC ACCTGGAATC TTTGAGTGAT TCTTTATATG ATAGCTTCTC TTCCTGTGCA
    AGTCAGGGTT CAAATGATGT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800416.2
    CDS623..6466
    Translation

    Target ORF information:

    RefSeq Version XM_010802114.3
    Organism Bos taurus(cattle)
    Definition Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802114.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGGGCA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TTTAGATAGC 
    AGTCTCGTGG AATACATGGA CTCCAACAAA TACATTGAAC ATCTGTTAAA TCAACTTGAG
    GAGCAACACA GAAGTCTCTG GAGGGAGAAG TTGGCTGTGG CCCGACTGCA GAGAGAAGTT
    GCCCAGAGAA GAAGTGAAGG GGCCATGCAT GAGAAGCTGA TACATGAATT GGAAGAGGAG
    AGACACTTGC GTCTTCAGAG TGAGAAGCGC TTGCAGGAGG TGACACTAGA GTCTGAGCGC
    AACAGAATTC AGATGCGCGG CCTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA
    AATCTACTAC AGAGTCAAGG ACCTCCGGAA CAGAAAAAAG AAGAAACTGT TAATATAATG
    GTCTATCAGG AAAAACTGTC AGAAGAAGAG CGAAAACACA AGGCGGCTTT GGAAGGCCGT
    CACATGGCGC TAGATGAGGA CTCGAGGAGT GAAGGGAGCA GTGCAGATGA GGGGAAAGGC
    AAGACCAAAT GGCTGTTAGA AAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCATTGGCT
    TTGGAAAATG AAAATCAAAG GGAACAGTAT GAGAGATGTC TTGATGAGGT AGCCAACCAG
    GTTGTGCAAG CCCTGCTCAC TCAAAAGGAT CTCAGGGAGG AATGTGTAAA GTTGAAAACA
    AGAGTGTTTG ATTTGGAACA ACAGAATCGA ACGCTAAGCA TCCTTTTCCA ACAGCGAGTC
    AGACCAACTT CTGATCTCCT CCTCCAGAAA CTTCACTCAC GCATCTTAGA TCTTTCATCC
    GGGGATTTAC TCTCAGATGT GGAAGGAAGC CAGAGTCTGA CGCACTCACG AACTGATGTT
    GAAATGCATG GCTGCCAGCT GAACACAAAA GCAGGAACCC CTGCTTTGAA ATGCCCTGGC
    ACGGGGATTG CCGTCTCTGG TCATCTCTGC CCTCGAAGCA GCTACAGCAG CAGTGAGCTG
    TCTCTTTCCA GCACCTGCAG CGAGTATTCC AGCGGCTCCT CCTACACGTG GCATGATGGA
    AAGAACCTCA GGAAAAGGTC ATCACAAAAT TGGGACAAAA GGTTAAGTAT TGACTCTTCG
    CTCCCGAGTG GCTTTGCTAG TCCTATGGAT GAACTACCTC CAACTCGCAT CAAGGAAAGC
    CACATTTTGG AAGGACTAAG AAAGCTCCAG AAGCGAAAAG TGTTACTTGA ACCCCCATCA
    GTGATAACCA AATGGGGTTA TAAAGATTGC ATGAACTCCA ATGAAGGAAT CTATTCTCCA
    GGAATTAATA GTAACAGCCT GAAGGAATAT CCACCCTGCA AACCCACAGA CACAAGGAGC
    CCCTGCACGG ATCCCCACAA GGCATTTGTT TATGACACGG ATTCTCATGA CGAGGCTGAT
    GAGGACGCTT CCTCCTTAGC CTCTGTCCAA GCGGTTCCAA ACCAGGGCTG CAGGCTGCAC
    AGTTGTAAAT TAACCCACAG TGTCTCTGAC AGCCTCTTTG GCTGGGAGCT GAATGGCAAA
    CACTGTTCAG AAGTCACTTC CTCGGTCTAC TCCAGGGAGA GGCCTGAGAA GCTGACCGGT
    TGTGCCAGCA ACTGTCCCTT GGAGCAGAAG CTGTGCCCAG GCATGCAGGG GCCTCGGGTA
    CAGAGAGAGA AGGTCTCACC CAGCCAGGGC TGCCAGGCCC TCAGTCTCCA GCCCTCAGAC
    ACCGATGACC CGGAGACCCT GGATGAGCTG CACATCGAGA GCAGTGATGA GAAAAGCCCT
    TCAGATGTGT CTGCAACCGC GGACACTGAC CAGTCCACGG AGAGCCTGGA TATCCTCACA
    GGATTTGAAA AATCTTCATG TGGGTCTCCC GAGGAGGAAG AAAACCAAGT ACCCGTCCAT
    TTAGAGAGCA GGCCAAAGAC GTTTAGTTTC ATTAAGCAAC AGAGGGTTGT AAAACGGACT
    TCCTCAGAAG AATGTATTAC TGTGATATTT GATGCAGAAG ACGGCGAGCC CATTGAGTTC
    AGCTCTCACC AGACTGGCCT TGTTACTGTT ACCAGAAAGG AGATTTCCAT CCATCAGGCC
    CCTGCTGGGC CCCAGATGGA ACACACTGAA CTTTCACCTC AGGGAATTGC TCATTTACAG
    CCAGGGGCTG CAGCTAGAGA CTACCCTTTT TTAAAGAGGT CTGAAGAGGA AACTGAAAGA
    AACATTCCAC GAGATGAGGT AGATGATCCT GCCATGACAC CTGCTGTGTC TTTCCACCCC
    AGGACAGTGA CGCAAAATAC TCAAAGACTG GCCAAACCGA CACATGTCAC ACCATGCCAG
    AGTCATTCTC GGTCATCTGT GGGCACAGGC GTCTGTCAGA AGAAAAGTCT GACAAAAATA
    CCTACCAGGG GCAAGTCTTC ACCTCAGAAA TCGAGAGTAA GGGAGCCTGA AGCCTCCCTT
    ATGATGCCCT CAGCTGGCTC AGTGACTCTT GAGAAATCAC CAGCTCCAGG GAAACTCTCA
    CAGTTCAAAA AGACTGAAGG TCCAGCTCCC CTTTTTGATT TGCAGCCAGA TTCACACATT
    CCAAAACCCC CCACCCAGCT TCCTCATGGC TCCAAGATGT CCAGCAGAAG GGATTGGGTC
    CAGAGCTCCA AGAATCAGAT GCTAGCATCA CAGTTGCTGT CCAGGCCCCC CACTGAGCGG
    AGTGACGATG GGGAGCCCCC CACGAGGGAT AAACACTGTG ATCCAGGGCC AGAGGCCGGG
    GTGAAATCGC CCTCCCCACC ATCCCCTCCA GGCAGGTCGG TCTCCCTACT CATCAGGCCC
    AGTTATGACT TTTTGCCACC ACCTTCATCT GCCAAATCCG AAAGCAGGGT CTCCACCGAG
    ACAGCAAGAA TGGTATTGAA ATCACCCCCT CTGAAAGGAT CCTCTGCTCC TGTTATTTAT
    TTTAACCAGA CCCTGACAGA TGTGCAGGGG AAGAAACCGT CTGTGGCCTT CAAAAAGCCC
    ATCTTCACTC CCTCACCACC CTCCGCAGAA ACAGCAATCC AGATGAGATG CCCTGCTCAC
    AGCCCCTCCA GCTCATTTGC TGTGATGGTC CCGGAGCCCC CAAAAGTCTC TCCAAAGAGA
    AATGCCCCCA GAGCCCCACC TCACCAAACA CTTGGGACCA CACAGAACAA CACAGGGTTA
    CAGACTCCCA AGAACTATGC TTCCCCCCGT GAGCCACTGG AAATACCATC CTCCAAAGGT
    GTATCTCCAG GGAGAAAAGA AAAGTTGAGT GGCAGTGTCT CTGCATCTTC CAAGCCTTCC
    TTCTTAGGGG TAAATGAGTC ACCATCATCT CAGGTTAACA GTCCCTTATC ATCAGCATCC
    TTCAAAAGCC ATAACCCCCT ACATGGTTGT CAAAACCTTC ATGAGAGAGG ACTGAAAACT
    CGCTTCCCAG TTGGGCTCAA AGTTTTCATG AAGTCTCCCC AGTTGCTCAG GAAAAGTTCC
    ACGGTGCCAG GGAAACAGGA AAAAGACAGT CTAAATGAAG CCTCGAAAAC CTCTGTGGCT
    GTGAGCAAGG CTAAGCCAGG GGCCTCCAGA AATCCAGCCA GCCTTGAGAC CACAGGAGGG
    GAAAGAAATA CGTCTCCAGT GAGTTTACCA GCACAAGAGA GTTTGGCCAA AGGGCTTCCC
    CTGGAAAGAG CAATGCCAGA GTCACTGGAA AATAGCATGC CTGGGGCTGA TAGAAAAGAT
    GGAGTAGAAA ACAGGTCTGT AAAAAGATCC CTGTCCTCCA GCAAACCACA CCTGAAACCA
    GCTCTAGGCA TGAACGGAGC CAAAGCCCGC AGCCAGAGTT TCAGTGCTCA CTCAGGGGAA
    AAGCCCCCCA CGCCCCCCAC AGAAGGGCTG GGCAAAGTCC GGACTCAAAT TATTACCAAC
    ACCGCTGAGA GAGGCAATTC CCTCACCCGG CAGAGCTCTT CTGCCGAAGG CTCTCCCAGC
    AAGAGTGCGT CTACCCCTGT GTCTGATGGT CTCCCCAGCA CTGGGAAGCC CCTGGGACAC
    CCTTCCCCCA GGCAGGGTTC CTTGGGGAGC ACAGGCAGCT CCAGCAGCCA GCATGGGAGC
    CCAAGCAAGT TGCCTTTGAG GATCCCTCCA AAATCCGAGG AGCCCCTCAC CCCAGCTGGA
    ACAGAAGAGC AGCAGGCCTA TGCCCAGGGA GAAGGCCCCA GGGTGACGGT ACCTGAGGAA
    CCAGGCAGCG ACCACTGCAG GTGCCCACCT ACCCCAACAG ATAGCTCAGG AGGCCCACAG
    AGCCTGGGGA GGACGCCACA TCCCAGCAGT TTCACAGCAA GCAGGACCTC CAAGCTAGAA
    ACTTCCGGAA GGTATCCAGA TACTTCTACA ACCAGGGCCG GTGTCGTAAG CCCAGAAGCC
    CCGCTCTCAC CCACAATTGA AGAAAAGGTC ATGTTGTGCA TTCAGGAAAA TGTGGAAAAG
    GGCCAAGTGC AAACAAAGTC CACCTCTGTG GAAGCAAAAC CGAAGCCGGG GCCTTCTTTT
    GCCAGCTGGT TCGGTTTTCG GAGGAGCAGA CTCCCGGCTC TCAGTAGCAG GAAGATGGAT
    GTCTCCAGAA CCAAAGCAGA AAAGAAAGAT GCAAAAGGTT TAGGGTTTGG AAACAAACAG
    CTGAAATCAG AGAGAAAGAA AGAGAAAAAG AAGCCTGAGC TACAGTGTGA GATGGAAAAT
    GAACTTCACA GGGATATCGA GTTGGCAGAT GGTCCAGACA GTGGTTTGCA AAACAGAAAT
    AACCCGAAAA CACCGCCAGA CATTTATGAT CAAGTGAAGT TTGAATCAAG AAATAGACCC
    AGCCCTGTTC CATGTTCAGC GAAAGATACC TTTATGACAG AACTTCTGAA CAGAGTTGAT
    AAGAAAGCAG CTCAACAGAC AGAAAGTGGA TCAAATAGTC TCTCCTGTAG GAGTGTGTTA
    AAGGGCAGCT CCCAGGGCTC CTGTCTCACC AGCAGCTCTC TCAGCACTCA AGGAAACCAC
    AAGAAAAACA TCAAAACCAA AGCAGATATG GAAAAACCGA AAGGCTCCCT GGTAAGAGAG
    GCAAATGATC ACCTGCAAGA GGATGAAGAA GATACAGTCG CAGATTCTGC ATTTCAGAGC
    CATACCATAG AAACAAACTG CCAGATGAGG ACCCTGGACA GTGGGATTGG AACCTTCCCA
    CTCCCAGACT CAGGAACTCG TGCAGCGGGA CGATACATAC GCCAAGCAGA CTCCCCTGAG
    GACACTGACC CCATCCTGTC TCTCCAACCG GCCCTTTGTG CAGCCTCTTC CATCAGAGCC
    CAGACCCTTG AAAGAGAAGT GCCTTCCTCT GCAGATAGCC AGGGCTCTGC AGACAATGCC
    ATTGTTCACT CCACATCCGA CCCCATCATG ACTGCCAGAG GGACGCGACC TCTTCAGAGT
    CTCCTCCCCA AACCAGCCTC CTCAGGAAAA ATCAATTCCC AAATGAAGAG TGAAGCCGAG
    CCAAGGTCTC AAAATTGTTC ACCATTCCAA TATGTCGAAA ACACAATGGC GAGCAAGCCA
    CTTCCAGCCT GGGAGGGTGA TGACGCTGCT GCAGAGACCC AGAAATTGAA ACAAGTTGAA
    GAAACCAGAG AAGATCCTGA GAATAGATTA TGCAAAATTT CCCTGGAGTC ATTCAATAAA
    TATGATGGCA ATACTGTGAT CTTACTAGGA AAGGAGAAGA ACTCTCTGAA CAAGGTTGAA
    GGACAAAAGG AAGAAAAAGA AAAAACTGAA GAGGCCTCTT TGAGTAGCTC TGAGAGACCT
    GGGGTAGACC ACCTGGAATC TTTGAGTGAT TCTTTATATG ATAGCTTCTC TTCCTGTGCA
    AGTCAGGGTT CAAATGATGT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227747
    Clone ID Related Accession (Same CDS sequence) XM_015475847.2
    Accession Version XM_015475847.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nck-associated protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475847.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAGGGCA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TTTAGATAGC 
    AGTCTCGTGG AATACATGGA CTCCAACAAA TACATTGAAC ATCTGTTAAA TCAACTTGAG
    GAGCAACACA GAAGTCTCTG GAGGGAGAAG TTGGCTGTGG CCCGACTGCA GAGAGAAGTT
    GCCCAGAGAA GAAGTGAAGG GGCCATGCAT GAGAAGCTGA TACATGAATT GGAAGAGGAG
    AGACACTTGC GTCTTCAGAG TGAGAAGCGC TTGCAGGAGG TGACACTAGA GTCTGAGCGC
    AACAGAATTC AGATGCGCGG CCTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA
    AATCTACTAC AGAGTCAAGG ACCTCCGGAA CAGAAAAAAG AAGAAACTGT TAATATAATG
    GTCTATCAGG AAAAACTGTC AGAAGAAGAG CGAAAACACA AGGCGGCTTT GGAAGGCCGT
    CACATGGCGC TAGATGAGGA CTCGAGGAGT GAAGGGAGCA GTGCAGATGA GGGGAAAGGC
    AAGACCAAAT GGCTGTTAGA AAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCATTGGCT
    TTGGAAAATG AAAATCAAAG GGAACAGTAT GAGAGATGTC TTGATGAGGT AGCCAACCAG
    GTTGTGCAAG CCCTGCTCAC TCAAAAGGAT CTCAGGGAGG AATGTGTAAA GTTGAAAACA
    AGAGTGTTTG ATTTGGAACA ACAGAATCGA ACGCTAAGCA TCCTTTTCCA ACAGCGAGTC
    AGACCAACTT CTGATCTCCT CCTCCAGGGC TGCCAGCTGA ACACAAAAGC AGGAACCCCT
    GCTTTGAAAT GCCCTGGCAC GGGGATTGCC GTCTCTGGTC ATCTCTGCCC TCGAAGCAGC
    TACAGCAGCA GTGAGCTGTC TCTTTCCAGC ACCTGCAGCG AGTATTCCAG CGGCTCCTCC
    TACACGTGGC ATGATGGAAA GAACCTCAGG AAAAGGCAGT CATCACAAAA TTGGGACAAA
    AGGTTAAGTA TTGACTCTTC GCTCCCGAGT GGCTTTGCTA GTCCTATGGA TGAACTACCT
    CCAACTCGCA TCAAGGAAAG CCACATTTTG GAAGGACTAA GAAAGCTCCA GAAGCGAAAA
    GTGTTACTTG AACCCCCATC AGTGATAACC AAATGGGGTT ATAAAGATTG CATGAACTCC
    AATGAAGGAA TCTATTCTCC AGGAATTAAT AGTAACAGCC TGAAGGAATA TCCACCCTGC
    AAACCCACAG ACACAAGGAG CCCCTGCACG GATCCCCACA AGGCATTTGT TTATGACACG
    GATTCTCATG ACGAGGCTGA TGAGGACGCT TCCTCCTTAG CCTCTGTCCA AGCGGTTCCA
    AACCAGGGCT GCAGGCTGCA CAGTTGTAAA TTAACCCACA GTGTCTCTGA CAGCCTCTTT
    GGCTGGGAGC TGAATGGCAA ACACTGTTCA GAAGTCACTT CCTCGGTCTA CTCCAGGGAG
    AGGCCTGAGA AGCTGACCGG TTGTGCCAGC AACTGTCCCT TGGAGCAGAA GCTGTGCCCA
    GGCATGCAGG GGCCTCGGGT ACAGAGAGAG AAGGTCTCAC CCAGCCAGGG CTGCCAGGCC
    CTCAGTCTCC AGCCCTCAGA CACCGATGAC CCGGAGACCC TGGATGAGCT GCACATCGAG
    AGCAGTGATG AGAAAAGCCC TTCAGATGTG TCTGCAACCG CGGACACTGA CCAGTCCACG
    GAGAGCCTGG ATATCCTCAC AGGATTTGAA AAATCTTCAT GTGGGTCTCC CGAGGAGGAA
    GAAAACCAAG TACCCGTCCA TTTAGAGAGC AGGCCAAAGA CGTTTAGTTT CATTAAGCAA
    CAGAGGGTTG TAAAACGGAC TTCCTCAGAA GAATGTATTA CTGTGATATT TGATGCAGAA
    GACGGCGAGC CCATTGAGTT CAGCTCTCAC CAGACTGGCC TTGTTACTGT TACCAGAAAG
    GAGATTTCCA TCCATCAGGC CCCTGCTGGG CCCCAGATGG AACACACTGA ACTTTCACCT
    CAGGGAATTG CTCATTTACA GCCAGGGGCT GCAGCTAGAG ACTACCCTTT TTTAAAGAGG
    TCTGAAGAGG AAACTGAAAG AAACATTCCA CGAGATGAGG TAGATGATCC TGCCATGACA
    CCTGCTGTGT CTTTCCACCC CAGGACAGTG ACGCAAAATA CTCAAAGACT GGCCAAACCG
    ACACATGTCA CACCATGCCA GAGTCATTCT CGGTCATCTG TGGGCACAGG CGTCTGTCAG
    AAGAAAAGTC TGACAAAAAT ACCTACCAGG GGCAAGTCTT CACCTCAGAA ATCGAGAGTA
    AGGGAGCCTG AAGCCTCCCT TATGATGCCC TCAGCTGGCT CAGTGACTCT TGAGAAATCA
    CCAGCTCCAG GGAAACTCTC ACAGTTCAAA AAGACTGAAG GTCCAGCTCC CCTTTTTGAT
    TTGCAGCCAG ATTCACACAT TCCAAAACCC CCCACCCAGC TTCCTCATGG CTCCAAGATG
    TCCAGCAGAA GGGATTGGGT CCAGAGCTCC AAGAATCAGA TGCTAGCATC ACAGTTGCTG
    TCCAGGCCCC CCACTGAGCG GAGTGACGAT GGGGAGCCCC CCACGAGGGA TAAACACTGT
    GATCCAGGGC CAGAGGCCGG GGTGAAATCG CCCTCCCCAC CATCCCCTCC AGGCAGGTCG
    GTCTCCCTAC TCATCAGGCC CAGTTATGAC TTTTTGCCAC CACCTTCATC TGCCAAATCC
    GAAAGCAGGG TCTCCACCGA GACAGCAAGA ATGGTATTGA AATCACCCCC TCTGAAAGGA
    TCCTCTGCTC CTGTTATTTA TTTTAACCAG ACCCTGACAG ATGTGCAGGG GAAGAAACCG
    TCTGTGGCCT TCAAAAAGCC CATCTTCACT CCCTCACCAC CCTCCGCAGA AACAGCAATC
    CAGATGAGAT GCCCTGCTCA CAGCCCCTCC AGCTCATTTG CTGTGATGGT CCCGGAGCCC
    CCAAAAGTCT CTCCAAAGAG AAATGCCCCC AGAGCCCCAC CTCACCAAAC ACTTGGGACC
    ACACAGAACA ACACAGGGTT ACAGACTCCC AAGAACTATG CTTCCCCCCG TGAGCCACTG
    GAAATACCAT CCTCCAAAGG TGTATCTCCA GGGAGAAAAG AAAAGTTGAG TGGCAGTGTC
    TCTGCATCTT CCAAGCCTTC CTTCTTAGGG GTAAATGAGT CACCATCATC TCAGGTTAAC
    AGTCCCTTAT CATCAGCATC CTTCAAAAGC CATAACCCCC TACATGGTTG TCAAAACCTT
    CATGAGAGAG GACTGAAAAC TCGCTTCCCA GTTGGGCTCA AAGTTTTCAT GAAGTCTCCC
    CAGTTGCTCA GGAAAAGTTC CACGGTGCCA GGGAAACAGG AAAAAGACAG TCTAAATGAA
    GCCTCGAAAA CCTCTGTGGC TGTGAGCAAG GCTAAGCCAG GGGCCTCCAG AAATCCAGCC
    AGCCTTGAGA CCACAGGAGG GGAAAGAAAT ACGTCTCCAG TGAGTTTACC AGCACAAGAG
    AGTTTGGCCA AAGGGCTTCC CCTGGAAAGA GCAATGCCAG AGTCACTGGA AAATAGCATG
    CCTGGGGCTG ATAGAAAAGA TGGAGTAGAA AACAGGTCTG TAAAAAGATC CCTGTCCTCC
    AGCAAACCAC ACCTGAAACC AGCTCTAGGC ATGAACGGAG CCAAAGCCCG CAGCCAGAGT
    TTCAGTGCTC ACTCAGGGGA AAAGCCCCCC ACGCCCCCCA CAGAAGGGCT GGGCAAAGTC
    CGGACTCAAA TTATTACCAA CACCGCTGAG AGAGGCAATT CCCTCACCCG GCAGAGCTCT
    TCTGCCGAAG GCTCTCCCAG CAAGAGTGCG TCTACCCCTG TGTCTGATGG TCTCCCCAGC
    ACTGGGAAGC CCCTGGGACA CCCTTCCCCC AGGCAGGGTT CCTTGGGGAG CACAGGCAGC
    TCCAGCAGCC AGCATGGGAG CCCAAGCAAG TTGCCTTTGA GGATCCCTCC AAAATCCGAG
    GAGCCCCTCA CCCCAGCTGG AACAGAAGAG CAGCAGGCCT ATGCCCAGGG AGAAGGCCCC
    AGGGTGACGG TACCTGAGGA ACCAGGCAGC GACCACTGCA GGTGCCCACC TACCCCAACA
    GATAGCTCAG GAGGCCCACA GAGCCTGGGG AGGACGCCAC ATCCCAGCAG TTTCACAGCA
    AGCAGGACCT CCAAGCTAGA AACTTCCGGA AGGTATCCAG ATACTTCTAC AACCAGGGCC
    GGTGTCGTAA GCCCAGAAGC CCCGCTCTCA CCCACAATTG AAGAAAAGGT CATGTTGTGC
    ATTCAGGAAA ATGTGGAAAA GGGCCAAGTG CAAACAAAGT CCACCTCTGT GGAAGCAAAA
    CCGAAGCCGG GGCCTTCTTT TGCCAGCTGG TTCGGTTTTC GGAGGAGCAG ACTCCCGGCT
    CTCAGTAGCA GGAAGATGGA TGTCTCCAGA ACCAAAGCAG AAAAGAAAGA TGCAAAAGGT
    TTAGGGTTTG GAAACAAACA GCTGAAATCA GAGAGAAAGA AAGAGAAAAA GAAGCCTGAG
    CTACAGTGTG AGATGGAAAA TGAACTTCAC AGGGATATCG AGTTGGCAGA TGGTCCAGAC
    AGTGGTTTGC AAAACAGAAA TAACCCGAAA ACACCGCCAG ACATTTATGA TCAAGTGAAG
    TTTGAATCAA GAAATAGACC CAGCCCTGTT CCATGTTCAG CGAAAGATAC CTTTATGACA
    GAACTTCTGA ACAGAGTTGA TAAGAAAGCA GCTCAACAGA CAGAAAGTGG ATCAAATAGT
    CTCTCCTGTA GGAGTGTGTT AAAGGGCAGC TCCCAGGGCT CCTGTCTCAC CAGCAGCTCT
    CTCAGCACTC AAGGAAACCA CAAGAAAAAC ATCAAAACCA AAGCAGATAT GGAAAAACCG
    AAAGGCTCCC TGGTAAGAGA GGCAAATGAT CACCTGCAAG AGGATGAAGA AGATACAGTC
    GCAGATTCTG CATTTCAGAG CCATACCATA GAAACAAACT GCCAGATGAG GACCCTGGAC
    AGTGGGATTG GAACCTTCCC ACTCCCAGAC TCAGGAACTC GTGCAGCGGG ACGATACATA
    CGCCAAGCAG ACTCCCCTGA GGACACTGAC CCCATCCTGT CTCTCCAACC GGCCCTTTGT
    GCAGCCTCTT CCATCAGAGC CCAGACCCTT GAAAGAGAAG TGCCTTCCTC TGCAGATAGC
    CAGGGCTCTG CAGACAATGC CATTGTTCAC TCCACATCCG ACCCCATCAT GACTGCCAGA
    GGGACGCGAC CTCTTCAGAG TCTCCTCCCC AAACCAGCCT CCTCAGGAAA AATCAATTCC
    CAAATGAAGA GTGAAGCCGA GCCAAGGTCT CAAAATTGTT CACCATTCCA ATATGTCGAA
    AACACAATGG CGAGCAAGCC ACTTCCAGCC TGGGAGGGTG ATGACGCTGC TGCAGAGACC
    CAGAAATTGA AACAAGTTGA AGAAACCAGA GAAGATCCTG AGAATAGATT ATGCAAAATT
    TCCCTGGAGT CATTCAATAA ATATGATGGC AATACTGTGA TCTTACTAGG AAAGGAGAAG
    AACTCTCTGA ACAAGGTTGA AGGACAAAAG GAAGAAAAAG AAAAAACTGA AGAGGCCTCT
    TTGAGTAGCT CTGAGAGACC TGGGGTAGAC CACCTGGAAT CTTTGAGTGA TTCTTTATAT
    GATAGCTTCT CTTCCTGTGC AAGTCAGGGT TCAAATGATG TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331333.2
    CDS623..6367
    Translation

    Target ORF information:

    RefSeq Version XM_015475847.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475847.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAGGGCA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TTTAGATAGC 
    AGTCTCGTGG AATACATGGA CTCCAACAAA TACATTGAAC ATCTGTTAAA TCAACTTGAG
    GAGCAACACA GAAGTCTCTG GAGGGAGAAG TTGGCTGTGG CCCGACTGCA GAGAGAAGTT
    GCCCAGAGAA GAAGTGAAGG GGCCATGCAT GAGAAGCTGA TACATGAATT GGAAGAGGAG
    AGACACTTGC GTCTTCAGAG TGAGAAGCGC TTGCAGGAGG TGACACTAGA GTCTGAGCGC
    AACAGAATTC AGATGCGCGG CCTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA
    AATCTACTAC AGAGTCAAGG ACCTCCGGAA CAGAAAAAAG AAGAAACTGT TAATATAATG
    GTCTATCAGG AAAAACTGTC AGAAGAAGAG CGAAAACACA AGGCGGCTTT GGAAGGCCGT
    CACATGGCGC TAGATGAGGA CTCGAGGAGT GAAGGGAGCA GTGCAGATGA GGGGAAAGGC
    AAGACCAAAT GGCTGTTAGA AAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCATTGGCT
    TTGGAAAATG AAAATCAAAG GGAACAGTAT GAGAGATGTC TTGATGAGGT AGCCAACCAG
    GTTGTGCAAG CCCTGCTCAC TCAAAAGGAT CTCAGGGAGG AATGTGTAAA GTTGAAAACA
    AGAGTGTTTG ATTTGGAACA ACAGAATCGA ACGCTAAGCA TCCTTTTCCA ACAGCGAGTC
    AGACCAACTT CTGATCTCCT CCTCCAGGGC TGCCAGCTGA ACACAAAAGC AGGAACCCCT
    GCTTTGAAAT GCCCTGGCAC GGGGATTGCC GTCTCTGGTC ATCTCTGCCC TCGAAGCAGC
    TACAGCAGCA GTGAGCTGTC TCTTTCCAGC ACCTGCAGCG AGTATTCCAG CGGCTCCTCC
    TACACGTGGC ATGATGGAAA GAACCTCAGG AAAAGGCAGT CATCACAAAA TTGGGACAAA
    AGGTTAAGTA TTGACTCTTC GCTCCCGAGT GGCTTTGCTA GTCCTATGGA TGAACTACCT
    CCAACTCGCA TCAAGGAAAG CCACATTTTG GAAGGACTAA GAAAGCTCCA GAAGCGAAAA
    GTGTTACTTG AACCCCCATC AGTGATAACC AAATGGGGTT ATAAAGATTG CATGAACTCC
    AATGAAGGAA TCTATTCTCC AGGAATTAAT AGTAACAGCC TGAAGGAATA TCCACCCTGC
    AAACCCACAG ACACAAGGAG CCCCTGCACG GATCCCCACA AGGCATTTGT TTATGACACG
    GATTCTCATG ACGAGGCTGA TGAGGACGCT TCCTCCTTAG CCTCTGTCCA AGCGGTTCCA
    AACCAGGGCT GCAGGCTGCA CAGTTGTAAA TTAACCCACA GTGTCTCTGA CAGCCTCTTT
    GGCTGGGAGC TGAATGGCAA ACACTGTTCA GAAGTCACTT CCTCGGTCTA CTCCAGGGAG
    AGGCCTGAGA AGCTGACCGG TTGTGCCAGC AACTGTCCCT TGGAGCAGAA GCTGTGCCCA
    GGCATGCAGG GGCCTCGGGT ACAGAGAGAG AAGGTCTCAC CCAGCCAGGG CTGCCAGGCC
    CTCAGTCTCC AGCCCTCAGA CACCGATGAC CCGGAGACCC TGGATGAGCT GCACATCGAG
    AGCAGTGATG AGAAAAGCCC TTCAGATGTG TCTGCAACCG CGGACACTGA CCAGTCCACG
    GAGAGCCTGG ATATCCTCAC AGGATTTGAA AAATCTTCAT GTGGGTCTCC CGAGGAGGAA
    GAAAACCAAG TACCCGTCCA TTTAGAGAGC AGGCCAAAGA CGTTTAGTTT CATTAAGCAA
    CAGAGGGTTG TAAAACGGAC TTCCTCAGAA GAATGTATTA CTGTGATATT TGATGCAGAA
    GACGGCGAGC CCATTGAGTT CAGCTCTCAC CAGACTGGCC TTGTTACTGT TACCAGAAAG
    GAGATTTCCA TCCATCAGGC CCCTGCTGGG CCCCAGATGG AACACACTGA ACTTTCACCT
    CAGGGAATTG CTCATTTACA GCCAGGGGCT GCAGCTAGAG ACTACCCTTT TTTAAAGAGG
    TCTGAAGAGG AAACTGAAAG AAACATTCCA CGAGATGAGG TAGATGATCC TGCCATGACA
    CCTGCTGTGT CTTTCCACCC CAGGACAGTG ACGCAAAATA CTCAAAGACT GGCCAAACCG
    ACACATGTCA CACCATGCCA GAGTCATTCT CGGTCATCTG TGGGCACAGG CGTCTGTCAG
    AAGAAAAGTC TGACAAAAAT ACCTACCAGG GGCAAGTCTT CACCTCAGAA ATCGAGAGTA
    AGGGAGCCTG AAGCCTCCCT TATGATGCCC TCAGCTGGCT CAGTGACTCT TGAGAAATCA
    CCAGCTCCAG GGAAACTCTC ACAGTTCAAA AAGACTGAAG GTCCAGCTCC CCTTTTTGAT
    TTGCAGCCAG ATTCACACAT TCCAAAACCC CCCACCCAGC TTCCTCATGG CTCCAAGATG
    TCCAGCAGAA GGGATTGGGT CCAGAGCTCC AAGAATCAGA TGCTAGCATC ACAGTTGCTG
    TCCAGGCCCC CCACTGAGCG GAGTGACGAT GGGGAGCCCC CCACGAGGGA TAAACACTGT
    GATCCAGGGC CAGAGGCCGG GGTGAAATCG CCCTCCCCAC CATCCCCTCC AGGCAGGTCG
    GTCTCCCTAC TCATCAGGCC CAGTTATGAC TTTTTGCCAC CACCTTCATC TGCCAAATCC
    GAAAGCAGGG TCTCCACCGA GACAGCAAGA ATGGTATTGA AATCACCCCC TCTGAAAGGA
    TCCTCTGCTC CTGTTATTTA TTTTAACCAG ACCCTGACAG ATGTGCAGGG GAAGAAACCG
    TCTGTGGCCT TCAAAAAGCC CATCTTCACT CCCTCACCAC CCTCCGCAGA AACAGCAATC
    CAGATGAGAT GCCCTGCTCA CAGCCCCTCC AGCTCATTTG CTGTGATGGT CCCGGAGCCC
    CCAAAAGTCT CTCCAAAGAG AAATGCCCCC AGAGCCCCAC CTCACCAAAC ACTTGGGACC
    ACACAGAACA ACACAGGGTT ACAGACTCCC AAGAACTATG CTTCCCCCCG TGAGCCACTG
    GAAATACCAT CCTCCAAAGG TGTATCTCCA GGGAGAAAAG AAAAGTTGAG TGGCAGTGTC
    TCTGCATCTT CCAAGCCTTC CTTCTTAGGG GTAAATGAGT CACCATCATC TCAGGTTAAC
    AGTCCCTTAT CATCAGCATC CTTCAAAAGC CATAACCCCC TACATGGTTG TCAAAACCTT
    CATGAGAGAG GACTGAAAAC TCGCTTCCCA GTTGGGCTCA AAGTTTTCAT GAAGTCTCCC
    CAGTTGCTCA GGAAAAGTTC CACGGTGCCA GGGAAACAGG AAAAAGACAG TCTAAATGAA
    GCCTCGAAAA CCTCTGTGGC TGTGAGCAAG GCTAAGCCAG GGGCCTCCAG AAATCCAGCC
    AGCCTTGAGA CCACAGGAGG GGAAAGAAAT ACGTCTCCAG TGAGTTTACC AGCACAAGAG
    AGTTTGGCCA AAGGGCTTCC CCTGGAAAGA GCAATGCCAG AGTCACTGGA AAATAGCATG
    CCTGGGGCTG ATAGAAAAGA TGGAGTAGAA AACAGGTCTG TAAAAAGATC CCTGTCCTCC
    AGCAAACCAC ACCTGAAACC AGCTCTAGGC ATGAACGGAG CCAAAGCCCG CAGCCAGAGT
    TTCAGTGCTC ACTCAGGGGA AAAGCCCCCC ACGCCCCCCA CAGAAGGGCT GGGCAAAGTC
    CGGACTCAAA TTATTACCAA CACCGCTGAG AGAGGCAATT CCCTCACCCG GCAGAGCTCT
    TCTGCCGAAG GCTCTCCCAG CAAGAGTGCG TCTACCCCTG TGTCTGATGG TCTCCCCAGC
    ACTGGGAAGC CCCTGGGACA CCCTTCCCCC AGGCAGGGTT CCTTGGGGAG CACAGGCAGC
    TCCAGCAGCC AGCATGGGAG CCCAAGCAAG TTGCCTTTGA GGATCCCTCC AAAATCCGAG
    GAGCCCCTCA CCCCAGCTGG AACAGAAGAG CAGCAGGCCT ATGCCCAGGG AGAAGGCCCC
    AGGGTGACGG TACCTGAGGA ACCAGGCAGC GACCACTGCA GGTGCCCACC TACCCCAACA
    GATAGCTCAG GAGGCCCACA GAGCCTGGGG AGGACGCCAC ATCCCAGCAG TTTCACAGCA
    AGCAGGACCT CCAAGCTAGA AACTTCCGGA AGGTATCCAG ATACTTCTAC AACCAGGGCC
    GGTGTCGTAA GCCCAGAAGC CCCGCTCTCA CCCACAATTG AAGAAAAGGT CATGTTGTGC
    ATTCAGGAAA ATGTGGAAAA GGGCCAAGTG CAAACAAAGT CCACCTCTGT GGAAGCAAAA
    CCGAAGCCGG GGCCTTCTTT TGCCAGCTGG TTCGGTTTTC GGAGGAGCAG ACTCCCGGCT
    CTCAGTAGCA GGAAGATGGA TGTCTCCAGA ACCAAAGCAG AAAAGAAAGA TGCAAAAGGT
    TTAGGGTTTG GAAACAAACA GCTGAAATCA GAGAGAAAGA AAGAGAAAAA GAAGCCTGAG
    CTACAGTGTG AGATGGAAAA TGAACTTCAC AGGGATATCG AGTTGGCAGA TGGTCCAGAC
    AGTGGTTTGC AAAACAGAAA TAACCCGAAA ACACCGCCAG ACATTTATGA TCAAGTGAAG
    TTTGAATCAA GAAATAGACC CAGCCCTGTT CCATGTTCAG CGAAAGATAC CTTTATGACA
    GAACTTCTGA ACAGAGTTGA TAAGAAAGCA GCTCAACAGA CAGAAAGTGG ATCAAATAGT
    CTCTCCTGTA GGAGTGTGTT AAAGGGCAGC TCCCAGGGCT CCTGTCTCAC CAGCAGCTCT
    CTCAGCACTC AAGGAAACCA CAAGAAAAAC ATCAAAACCA AAGCAGATAT GGAAAAACCG
    AAAGGCTCCC TGGTAAGAGA GGCAAATGAT CACCTGCAAG AGGATGAAGA AGATACAGTC
    GCAGATTCTG CATTTCAGAG CCATACCATA GAAACAAACT GCCAGATGAG GACCCTGGAC
    AGTGGGATTG GAACCTTCCC ACTCCCAGAC TCAGGAACTC GTGCAGCGGG ACGATACATA
    CGCCAAGCAG ACTCCCCTGA GGACACTGAC CCCATCCTGT CTCTCCAACC GGCCCTTTGT
    GCAGCCTCTT CCATCAGAGC CCAGACCCTT GAAAGAGAAG TGCCTTCCTC TGCAGATAGC
    CAGGGCTCTG CAGACAATGC CATTGTTCAC TCCACATCCG ACCCCATCAT GACTGCCAGA
    GGGACGCGAC CTCTTCAGAG TCTCCTCCCC AAACCAGCCT CCTCAGGAAA AATCAATTCC
    CAAATGAAGA GTGAAGCCGA GCCAAGGTCT CAAAATTGTT CACCATTCCA ATATGTCGAA
    AACACAATGG CGAGCAAGCC ACTTCCAGCC TGGGAGGGTG ATGACGCTGC TGCAGAGACC
    CAGAAATTGA AACAAGTTGA AGAAACCAGA GAAGATCCTG AGAATAGATT ATGCAAAATT
    TCCCTGGAGT CATTCAATAA ATATGATGGC AATACTGTGA TCTTACTAGG AAAGGAGAAG
    AACTCTCTGA ACAAGGTTGA AGGACAAAAG GAAGAAAAAG AAAAAACTGA AGAGGCCTCT
    TTGAGTAGCT CTGAGAGACC TGGGGTAGAC CACCTGGAAT CTTTGAGTGA TTCTTTATAT
    GATAGCTTCT CTTCCTGTGC AAGTCAGGGT TCAAATGATG TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227748
    Clone ID Related Accession (Same CDS sequence) XM_024980966.1
    Accession Version XM_024980966.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5715bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nck-associated protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAGGGCA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TTTAGATAGC 
    AGTCTCGTGG AATACATGGA CTCCAACAAA TACATTGAAC ATCTGTTAAA TCAACTTGAG
    GAGCAACACA GAAGTCTCTG GAGGGAGAAG TTGGCTGTGG CCCGACTGCA GAGAGAAGTT
    GCCCAGAGAA GAAGTGAAGG GGCCATGCAT GAGAAGCTGA TACATGAATT GGAAGAGGAG
    AGACACTTGC GTCTTCAGAG TGAGAAGCGC TTGCAGGAGG TGACACTAGA GTCTGAGCGC
    AACAGAATTC AGATGCGCGG CCTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA
    AATCTACTAC AGAGTCAAGG ACCTCCGGAA CAGAAAAAAG AAGAAACTGT TAATATAATG
    GTCTATCAGG AAAAACTGTC AGAAGAAGAG CGAAAACACA AGGCGGCTTT GGAAGGCCGT
    CACATGGCGC TAGATGAGGA CTCGAGGAGT GAAGGGAGCA GTGCAGATGA GGGGAAAGGC
    AAGACCAAAT GGCTGTTAGA AAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCATTGGCT
    TTGGAAAATG AAAATCAAAG GGAACAGTAT GAGAGATGTC TTGATGAGGT AGCCAACCAG
    GTTGTGCAAG CCCTGCTCAC TCAAAAGGAT CTCAGGGAGG AATGTGTAAA GTTGAAAACA
    AGAGTGTTTG ATTTGGAACA ACAGAATCGA ACGCTAAGCA TCCTTTTCCA ACAGCGAGTC
    AGACCAACTT CTGATCTCCT CCTCCAGAAA CTTCACTCAC GCATCTTAGA TCTTTCATCC
    GGGGATTTAC TCTCAGATGT GGAAGGAAGC CAGAGTCTGA CGCACTCACG AACTGATGTT
    GAAATGCATG GCTGCCAGCT GAACACAAAA GCAGGAACCC CTGCTTTGAA ATGCCCTGGC
    ACGGGGATTG CCGTCTCTGG TCATCTCTGC CCTCGAAGCA GCTACAGCAG CAGTGAGCTG
    TCTCTTTCCA GCACCTGCAG CGAGTATTCC AGCGGCTCCT CCTACACGTG GCATGATGGA
    AAGAACCTCA GGAAAAGGCA GTCATCACAA AATTGGGACA AAAGGTTAAG TATTGACTCT
    TCGCTCCCGA GTGGCTTTGC TAGTCCTATG GATGAACTAC CTCCAACTCG CATCAAGGAA
    AGCCACATTT TGGAAGGACT AAGAAAGCTC CAGAAGCGAA AAGTGTTACT TGAACCCCCA
    TCAGTGATAA CCAAATGGGG TTATAAAGAT TGCATGAACT CCAATGAAGG AATCTATTCT
    CCAGGAATTA ATAGTAACAG CCTGAAGGAA TATCCACCCT GCAAACCCAC AGACACAAGG
    AGCCCCTGCA CGGATCCCCA CAAGGCATTT GTTTATGACA CGGATTCTCA TGACGAGGCT
    GATGAGGACG CTTCCTCCTT AGCCTCTGTC CAAGCGGTTC CAAACCAGGG CTGCAGGCTG
    CACAGTTGTA AATTAACCCA CAGTGTCTCT GACAGCCTCT TTGGCTGGGA GCTGAATGGC
    AAACACTGTT CAGAAGTCAC TTCCTCGGTC TACTCCAGGG AGAGGCCTGA GAAGCTGACC
    GGTTGTGCCA GCAACTGTCC CTTGGAGCAG AAGCTGTGCC CAGGCATGCA GGGGCCTCGG
    GTACAGAGAG AGAAGGTCTC ACCCAGCCAG GGCTGCCAGG CCCTCAGTCT CCAGCCCTCA
    GACACCGATG ACCCGGAGAC CCTGGATGAG CTGCACATCG AGAGCAGTGA TGAGAAAAGC
    CCTTCAGATG TGTCTGCAAC CGCGGACACT GACCAGTCCA CGGAGAGCCT GGATATCCTC
    ACAGGATTTG AAAAATCTTC ATGTGGGTCT CCCGAGGAGG AAGAAAACCA AGTACCCGTC
    CATTTAGAGA GCAGGCCAAA GACGTTTAGT TTCATTAAGC AACAGAGGGT TGTAAAACGG
    ACTTCCTCAG AAGAATGTAT TACTGTGATA TTTGATGCAG AAGACGGCGA GCCCATTGAG
    TTCAGCTCTC ACCAGACTGG CCTTGTTACT GTTACCAGAA AGGAGATTTC CATCCATCAG
    GCCCCTGCTG GGCCCCAGAT GGAACACACT GAACTTTCAC CTCAGGGAAT TGCTCATTTA
    CAGCCAGGGG CTGCAGCTAG AGACTACCCT TTTTTAAAGA GGTCTGAAGA GGAAACTGAA
    AGAAACATTC CACGAGATGA GGTAGATGAT CCTGCCATGA CACCTGCTGT GTCTTTCCAC
    CCCAGGACAG TGACGCAAAA TACTCAAAGA CTGGCCAAAC CGACACATGT CACACCATGC
    CAGAGTCATT CTCGGTCATC TGTGGGCACA GGCGTCTGTC AGAAGAAAAG TCTGACAAAA
    ATACCTACCA GGGGCAAGTC TTCACCTCAG AAATCGAGAG TAAGGGAGCC TGAAGCCTCC
    CTTATGATGC CCTCAGCTGG CTCAGTGACT CTTGAGAAAT CACCAGCTCC AGGGAAACTC
    TCACAGTTCA AAAAGACTGA AGGTCCAGCT CCCCTTTTTG ATTTGCAGCC AGATTCACAC
    ATTCCAAAAC CCCCCACCCA GCTTCCTCAT GGCTCCAAGA TGTCCAGCAG AAGGGATTGG
    GTCCAGAGCT CCAAGAATCA GATGCTAGCA TCACAGTTGC TGTCCAGGCC CCCCACTGAG
    CGGAGTGACG ATGGGGAGCC CCCCACGAGG GATAAACACT GTGATCCAGG GCCAGAGGCC
    GGGGTGAAAT CGCCCTCCCC ACCATCCCCT CCAGGCAGGT CGGTCTCCCT ACTCATCAGG
    CCCAGTTATG ACTTTTTGCC ACCACCTTCA TCTGCCAAAT CCGAAAGCAG GGTCTCCACC
    GAGACAGCAA GAATGGTATT GAAATCACCC CCTCTGAAAG GATCCTCTGC TCCTGTTATT
    TATTTTAACC AGACCCTGAC AGATGTGCAG GGGAAGAAAC CGTCTGTGGC CTTCAAAAAG
    CCCATCTTCA CTCCCTCACC ACCCTCCGCA GAAACAGCAA TCCAGATGAG ATGCCCTGCT
    CACAGCCCCT CCAGCTCATT TGCTGTGATG GTCCCGGAGC CCCCAAAAGT CTCTCCAAAG
    AGAAATGCCC CCAGAGCCCC ACCTCACCAA ACACTTGGGA CCACACAGAA CAACACAGGG
    TTACAGACTC CCAAGAACTA TGCTTCCCCC CGTGAGCCAC TGGAAATACC ATCCTCCAAA
    GGTGTATCTC CAGGGAGAAA AGAAAAGTTG AGTGGCAGTG TCTCTGCATC TTCCAAGCCT
    TCCTTCTTAG GGGTAAATGA GTCACCATCA TCTCAGGTTA ACAGTCCCTT ATCATCAGCA
    TCCTTCAAAA GCCATAACCC CCTACATGGT TGTCAAAACC TTCATGAGAG AGGACTGAAA
    ACTCGCTTCC CAGTTGGGCT CAAAGTTTTC ATGAAGTCTC CCCAGTTGCT CAGGAAAAGT
    TCCACGGTGC CAGGGAAACA GGAAAAAGAC AGTCTAAATG AAGCCTCGAA AACCTCTGTG
    GCTGTGAGCA AGGCTAAGCC AGGGGCCTCC AGAAATCCAG CCAGCCTTGA GACCACAGGA
    GGGGAAAGAA ATACGTCTCC AGTGAGTTTA CCAGCACAAG AGAGTTTGGC CAAAGGGCTT
    CCCCTGGAAA GAGCAATGCC AGAGTCACTG GAAAATAGCA TGCCTGGGGC TGATAGAAAA
    GATGGAGTAG AAAACAGGTC TGTAAAAAGA TCCCTGTCCT CCAGCAAACC ACACCTGAAA
    CCAGCTCTAG GCATGAACGG AGCCAAAGCC CGCAGCCAGA GTTTCAGTGC TCACTCAGGG
    GAAAAGCCCC CCACGCCCCC CACAGAAGGG CTGGGCAAAG TCCGGACTCA AATTATTACC
    AACACCGCTG AGAGAGGCAA TTCCCTCACC CGGCAGAGCT CTTCTGCCGA AGGCTCTCCC
    AGCAAGAGTG CGTCTACCCC TGTGTCTGAT GGTCTCCCCA GCACTGGGAA GCCCCTGGGA
    CACCCTTCCC CCAGGCAGGG TTCCTTGGGG AGCACAGGCA GCTCCAGCAG CCAGCATGGG
    AGCCCAAGCA AGTTGCCTTT GAGGATCCCT CCAAAATCCG AGGAGCCCCT CACCCCAGCT
    GGAACAGAAG AGCAGCAGGC CTATGCCCAG GGAGAAGGCC CCAGGGTGAC GGTACCTGAG
    GAACCAGGCA GCGACCACTG CAGGTGCCCA CCTACCCCAA CAGATAGCTC AGGAGGCCCA
    CAGAGCCTGG GGAGGACGCC ACATCCCAGC AGTTTCACAG CAAGCAGGAC CTCCAAGCTA
    GAAACTTCCG GAAGGTATCC AGATACTTCT ACAACCAGGG CCGGTGTCGT AAGCCCAGAA
    GCCCCGCTCT CACCCACAAT TGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA
    AAGGGCCAAG TGCAAACAAA GTCCACCTCT GTGGAAGCAA AACCGAAGCC GGGGCCTTCT
    TTTGCCAGCT GGTTCGGTTT TCGGAGGAGC AGACTCCCGG CTCTCAGTAG CAGGAAGATG
    GATGTCTCCA GAACCAAAGC AGAAAAGAAA GATGCAAAAG GTTTAGGGTT TGGAAACAAA
    CAGCTGAAAT CAGAGAGAAA GAAAGAGAAA AAGAAGCCTG AGCTACAGTG TGAGATGGAA
    AATGAACTTC ACAGGGATAT CGAGTTGGCA GATGGTCCAG ACAGTGGTTT GCAAAACAGA
    AATAACCCGA AAACACCGCC AGACATTTAT GATCAAGTGA AGTTTGAATC AAGAAATAGA
    CCCAGCCCTG TTCCATGTTC AGCGAAAGAT ACCTTTATGA CAGAACTTCT GAACAGAGTT
    GATAAGAAAG CAGCTCAACA GACAGAAAGT GGATCAAATA GTCTCTCCTG TAGGAGTGTG
    TTAAAGGGCA GCTCCCAGGG CTCCTGTCTC ACCAGCAGCT CTCTCAGCAC TCAAGGAAAC
    CACAAGAAAA ACATCAAAAC CAAAGCAGAT ATGGAAAAAC CGAAAGGCTC CCTGGTAAGA
    GAGGCAAATG ATCACCTGCA AGAGGATGAA GAAGATACAG TCGCAGATTC TGCATTTCAG
    AGCCATACCA TAGAAACAAA CTGCCAGATG AGGACCCTGG ACAGTGGGAT TGGAACCTTC
    CCACTCCCAG ACTCAGGAAC TCGTGCAGCG GGACGATACA TACGCCAAGC AGACTCCCCT
    GAGGACACTG ACCCCATCCT GTCTCTCCAA CCGGCCCTTT GTGCAGCCTC TTCCATCAGA
    GCCCAGACCC TTGAAAGAGA AGTGCCTTCC TCTGCAGATA GCCAGGGCTC TGCAGACAAT
    GCCATTGTTC ACTCCACATC CGACCCCATC ATGACTGCCA GAGGGACGCG ACCTCTTCAG
    AGTCTCCTCC CCAAACCAGC CTCCTCAGGA AAAATCAATT CCCAAATGAA GAGTGAAGCC
    GAGCCAAGGT CTCAAAATTG TTCACCATTC CAATATGTCG AAAACACAAT GGCGAGCAAG
    CCACTTCCAG CCTGGGAGGG TGATGACGCT GCTGCAGAGA CCCAGGACAA GGCACCCAGA
    ATGTGTGCAT ACTCTGCCAG CGGTAGCAGT GATAGTGACA GTGACCCGGA CTATGGAAAT
    AATGGTTTTG GAGCTGGAAG GGGCAAGTTA GTGAAAGCAA TGAAGAGCAC CACCCCAGAA
    ATTGAAACAA GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836734.1
    CDS624..6338
    Translation

    Target ORF information:

    RefSeq Version XM_024980966.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980966.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAGGGCA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TTTAGATAGC 
    AGTCTCGTGG AATACATGGA CTCCAACAAA TACATTGAAC ATCTGTTAAA TCAACTTGAG
    GAGCAACACA GAAGTCTCTG GAGGGAGAAG TTGGCTGTGG CCCGACTGCA GAGAGAAGTT
    GCCCAGAGAA GAAGTGAAGG GGCCATGCAT GAGAAGCTGA TACATGAATT GGAAGAGGAG
    AGACACTTGC GTCTTCAGAG TGAGAAGCGC TTGCAGGAGG TGACACTAGA GTCTGAGCGC
    AACAGAATTC AGATGCGCGG CCTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA
    AATCTACTAC AGAGTCAAGG ACCTCCGGAA CAGAAAAAAG AAGAAACTGT TAATATAATG
    GTCTATCAGG AAAAACTGTC AGAAGAAGAG CGAAAACACA AGGCGGCTTT GGAAGGCCGT
    CACATGGCGC TAGATGAGGA CTCGAGGAGT GAAGGGAGCA GTGCAGATGA GGGGAAAGGC
    AAGACCAAAT GGCTGTTAGA AAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCATTGGCT
    TTGGAAAATG AAAATCAAAG GGAACAGTAT GAGAGATGTC TTGATGAGGT AGCCAACCAG
    GTTGTGCAAG CCCTGCTCAC TCAAAAGGAT CTCAGGGAGG AATGTGTAAA GTTGAAAACA
    AGAGTGTTTG ATTTGGAACA ACAGAATCGA ACGCTAAGCA TCCTTTTCCA ACAGCGAGTC
    AGACCAACTT CTGATCTCCT CCTCCAGAAA CTTCACTCAC GCATCTTAGA TCTTTCATCC
    GGGGATTTAC TCTCAGATGT GGAAGGAAGC CAGAGTCTGA CGCACTCACG AACTGATGTT
    GAAATGCATG GCTGCCAGCT GAACACAAAA GCAGGAACCC CTGCTTTGAA ATGCCCTGGC
    ACGGGGATTG CCGTCTCTGG TCATCTCTGC CCTCGAAGCA GCTACAGCAG CAGTGAGCTG
    TCTCTTTCCA GCACCTGCAG CGAGTATTCC AGCGGCTCCT CCTACACGTG GCATGATGGA
    AAGAACCTCA GGAAAAGGCA GTCATCACAA AATTGGGACA AAAGGTTAAG TATTGACTCT
    TCGCTCCCGA GTGGCTTTGC TAGTCCTATG GATGAACTAC CTCCAACTCG CATCAAGGAA
    AGCCACATTT TGGAAGGACT AAGAAAGCTC CAGAAGCGAA AAGTGTTACT TGAACCCCCA
    TCAGTGATAA CCAAATGGGG TTATAAAGAT TGCATGAACT CCAATGAAGG AATCTATTCT
    CCAGGAATTA ATAGTAACAG CCTGAAGGAA TATCCACCCT GCAAACCCAC AGACACAAGG
    AGCCCCTGCA CGGATCCCCA CAAGGCATTT GTTTATGACA CGGATTCTCA TGACGAGGCT
    GATGAGGACG CTTCCTCCTT AGCCTCTGTC CAAGCGGTTC CAAACCAGGG CTGCAGGCTG
    CACAGTTGTA AATTAACCCA CAGTGTCTCT GACAGCCTCT TTGGCTGGGA GCTGAATGGC
    AAACACTGTT CAGAAGTCAC TTCCTCGGTC TACTCCAGGG AGAGGCCTGA GAAGCTGACC
    GGTTGTGCCA GCAACTGTCC CTTGGAGCAG AAGCTGTGCC CAGGCATGCA GGGGCCTCGG
    GTACAGAGAG AGAAGGTCTC ACCCAGCCAG GGCTGCCAGG CCCTCAGTCT CCAGCCCTCA
    GACACCGATG ACCCGGAGAC CCTGGATGAG CTGCACATCG AGAGCAGTGA TGAGAAAAGC
    CCTTCAGATG TGTCTGCAAC CGCGGACACT GACCAGTCCA CGGAGAGCCT GGATATCCTC
    ACAGGATTTG AAAAATCTTC ATGTGGGTCT CCCGAGGAGG AAGAAAACCA AGTACCCGTC
    CATTTAGAGA GCAGGCCAAA GACGTTTAGT TTCATTAAGC AACAGAGGGT TGTAAAACGG
    ACTTCCTCAG AAGAATGTAT TACTGTGATA TTTGATGCAG AAGACGGCGA GCCCATTGAG
    TTCAGCTCTC ACCAGACTGG CCTTGTTACT GTTACCAGAA AGGAGATTTC CATCCATCAG
    GCCCCTGCTG GGCCCCAGAT GGAACACACT GAACTTTCAC CTCAGGGAAT TGCTCATTTA
    CAGCCAGGGG CTGCAGCTAG AGACTACCCT TTTTTAAAGA GGTCTGAAGA GGAAACTGAA
    AGAAACATTC CACGAGATGA GGTAGATGAT CCTGCCATGA CACCTGCTGT GTCTTTCCAC
    CCCAGGACAG TGACGCAAAA TACTCAAAGA CTGGCCAAAC CGACACATGT CACACCATGC
    CAGAGTCATT CTCGGTCATC TGTGGGCACA GGCGTCTGTC AGAAGAAAAG TCTGACAAAA
    ATACCTACCA GGGGCAAGTC TTCACCTCAG AAATCGAGAG TAAGGGAGCC TGAAGCCTCC
    CTTATGATGC CCTCAGCTGG CTCAGTGACT CTTGAGAAAT CACCAGCTCC AGGGAAACTC
    TCACAGTTCA AAAAGACTGA AGGTCCAGCT CCCCTTTTTG ATTTGCAGCC AGATTCACAC
    ATTCCAAAAC CCCCCACCCA GCTTCCTCAT GGCTCCAAGA TGTCCAGCAG AAGGGATTGG
    GTCCAGAGCT CCAAGAATCA GATGCTAGCA TCACAGTTGC TGTCCAGGCC CCCCACTGAG
    CGGAGTGACG ATGGGGAGCC CCCCACGAGG GATAAACACT GTGATCCAGG GCCAGAGGCC
    GGGGTGAAAT CGCCCTCCCC ACCATCCCCT CCAGGCAGGT CGGTCTCCCT ACTCATCAGG
    CCCAGTTATG ACTTTTTGCC ACCACCTTCA TCTGCCAAAT CCGAAAGCAG GGTCTCCACC
    GAGACAGCAA GAATGGTATT GAAATCACCC CCTCTGAAAG GATCCTCTGC TCCTGTTATT
    TATTTTAACC AGACCCTGAC AGATGTGCAG GGGAAGAAAC CGTCTGTGGC CTTCAAAAAG
    CCCATCTTCA CTCCCTCACC ACCCTCCGCA GAAACAGCAA TCCAGATGAG ATGCCCTGCT
    CACAGCCCCT CCAGCTCATT TGCTGTGATG GTCCCGGAGC CCCCAAAAGT CTCTCCAAAG
    AGAAATGCCC CCAGAGCCCC ACCTCACCAA ACACTTGGGA CCACACAGAA CAACACAGGG
    TTACAGACTC CCAAGAACTA TGCTTCCCCC CGTGAGCCAC TGGAAATACC ATCCTCCAAA
    GGTGTATCTC CAGGGAGAAA AGAAAAGTTG AGTGGCAGTG TCTCTGCATC TTCCAAGCCT
    TCCTTCTTAG GGGTAAATGA GTCACCATCA TCTCAGGTTA ACAGTCCCTT ATCATCAGCA
    TCCTTCAAAA GCCATAACCC CCTACATGGT TGTCAAAACC TTCATGAGAG AGGACTGAAA
    ACTCGCTTCC CAGTTGGGCT CAAAGTTTTC ATGAAGTCTC CCCAGTTGCT CAGGAAAAGT
    TCCACGGTGC CAGGGAAACA GGAAAAAGAC AGTCTAAATG AAGCCTCGAA AACCTCTGTG
    GCTGTGAGCA AGGCTAAGCC AGGGGCCTCC AGAAATCCAG CCAGCCTTGA GACCACAGGA
    GGGGAAAGAA ATACGTCTCC AGTGAGTTTA CCAGCACAAG AGAGTTTGGC CAAAGGGCTT
    CCCCTGGAAA GAGCAATGCC AGAGTCACTG GAAAATAGCA TGCCTGGGGC TGATAGAAAA
    GATGGAGTAG AAAACAGGTC TGTAAAAAGA TCCCTGTCCT CCAGCAAACC ACACCTGAAA
    CCAGCTCTAG GCATGAACGG AGCCAAAGCC CGCAGCCAGA GTTTCAGTGC TCACTCAGGG
    GAAAAGCCCC CCACGCCCCC CACAGAAGGG CTGGGCAAAG TCCGGACTCA AATTATTACC
    AACACCGCTG AGAGAGGCAA TTCCCTCACC CGGCAGAGCT CTTCTGCCGA AGGCTCTCCC
    AGCAAGAGTG CGTCTACCCC TGTGTCTGAT GGTCTCCCCA GCACTGGGAA GCCCCTGGGA
    CACCCTTCCC CCAGGCAGGG TTCCTTGGGG AGCACAGGCA GCTCCAGCAG CCAGCATGGG
    AGCCCAAGCA AGTTGCCTTT GAGGATCCCT CCAAAATCCG AGGAGCCCCT CACCCCAGCT
    GGAACAGAAG AGCAGCAGGC CTATGCCCAG GGAGAAGGCC CCAGGGTGAC GGTACCTGAG
    GAACCAGGCA GCGACCACTG CAGGTGCCCA CCTACCCCAA CAGATAGCTC AGGAGGCCCA
    CAGAGCCTGG GGAGGACGCC ACATCCCAGC AGTTTCACAG CAAGCAGGAC CTCCAAGCTA
    GAAACTTCCG GAAGGTATCC AGATACTTCT ACAACCAGGG CCGGTGTCGT AAGCCCAGAA
    GCCCCGCTCT CACCCACAAT TGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA
    AAGGGCCAAG TGCAAACAAA GTCCACCTCT GTGGAAGCAA AACCGAAGCC GGGGCCTTCT
    TTTGCCAGCT GGTTCGGTTT TCGGAGGAGC AGACTCCCGG CTCTCAGTAG CAGGAAGATG
    GATGTCTCCA GAACCAAAGC AGAAAAGAAA GATGCAAAAG GTTTAGGGTT TGGAAACAAA
    CAGCTGAAAT CAGAGAGAAA GAAAGAGAAA AAGAAGCCTG AGCTACAGTG TGAGATGGAA
    AATGAACTTC ACAGGGATAT CGAGTTGGCA GATGGTCCAG ACAGTGGTTT GCAAAACAGA
    AATAACCCGA AAACACCGCC AGACATTTAT GATCAAGTGA AGTTTGAATC AAGAAATAGA
    CCCAGCCCTG TTCCATGTTC AGCGAAAGAT ACCTTTATGA CAGAACTTCT GAACAGAGTT
    GATAAGAAAG CAGCTCAACA GACAGAAAGT GGATCAAATA GTCTCTCCTG TAGGAGTGTG
    TTAAAGGGCA GCTCCCAGGG CTCCTGTCTC ACCAGCAGCT CTCTCAGCAC TCAAGGAAAC
    CACAAGAAAA ACATCAAAAC CAAAGCAGAT ATGGAAAAAC CGAAAGGCTC CCTGGTAAGA
    GAGGCAAATG ATCACCTGCA AGAGGATGAA GAAGATACAG TCGCAGATTC TGCATTTCAG
    AGCCATACCA TAGAAACAAA CTGCCAGATG AGGACCCTGG ACAGTGGGAT TGGAACCTTC
    CCACTCCCAG ACTCAGGAAC TCGTGCAGCG GGACGATACA TACGCCAAGC AGACTCCCCT
    GAGGACACTG ACCCCATCCT GTCTCTCCAA CCGGCCCTTT GTGCAGCCTC TTCCATCAGA
    GCCCAGACCC TTGAAAGAGA AGTGCCTTCC TCTGCAGATA GCCAGGGCTC TGCAGACAAT
    GCCATTGTTC ACTCCACATC CGACCCCATC ATGACTGCCA GAGGGACGCG ACCTCTTCAG
    AGTCTCCTCC CCAAACCAGC CTCCTCAGGA AAAATCAATT CCCAAATGAA GAGTGAAGCC
    GAGCCAAGGT CTCAAAATTG TTCACCATTC CAATATGTCG AAAACACAAT GGCGAGCAAG
    CCACTTCCAG CCTGGGAGGG TGATGACGCT GCTGCAGAGA CCCAGGACAA GGCACCCAGA
    ATGTGTGCAT ACTCTGCCAG CGGTAGCAGT GATAGTGACA GTGACCCGGA CTATGGAAAT
    AATGGTTTTG GAGCTGGAAG GGGCAAGTTA GTGAAAGCAA TGAAGAGCAC CACCCCAGAA
    ATTGAAACAA GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87935
    Clone ID Related Accession (Same CDS sequence) XM_015475847.1 , XM_015462321.1
    Accession Version XM_015475847.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nck-associated protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103841.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGCTAG ATGAGGACTC GAGGAGTGAA GGGAGCAGTG CAGATGAGGG GAAAGGCAAG 
    ACCAAATGGC TGTTAGAAAG ATTGAAAGCT CTAGAGGCAG AGAATTCAGC ATTGGCTTTG
    GAAAATGAAA ATCAAAGGGA ACAGTATGAG AGATGTCTTG ATGAGGTAGC CAACCAGGTT
    GTGCAAGCCC TGCTCACTCA AAAGGATCTC AGGGAGGAAT GTGTAAAGTT GAAAACAAGA
    GTGTTTGATT TGGAACAACA GAATCGAACG CTAAGCATCC TTTTCCAACA GCGAGTCAGA
    CCAACTTCTG ATCTCCTCCT CCAGGGCTGC CAGCTGAACA CAAAAGCAGG AACCCCTGCT
    TTGAAATGCC CTGGCACGGG GATTGCCGTC TCTGGTCATC TCTGCCCTCG AAGCAGCTAC
    AGCAGCAGTG AGCTGTCTCT TTCCAGCACC TGCAGCGAGT ATTCCAGCAG CTCCTCCTAC
    ACGTGGCATG ATGGAAAGAA CCTCAGGAAA AGGCAGTCAT CACAAAATTG GGACAAAAGG
    TTAAGTATTG ACTCTTCGCT CCCGAGTGGC TTTGCTAGTC CTATGGATGA ACTACCTCCA
    ACTCGCATCA AGGAAAGCCA CATTTTGGAA GGACTAAGAA AGCTCCAGAA GCGAAAAGTG
    TTACTTGAAC CCCCATCAGT GATAACCAAA TGGGGTTATA AAGATTGCAT GAACTCCAAT
    GAAGGAATCT ATTCTCCAGG AATTAATAGT AACAGCCTGA AGGAATATCC ACCCTGCAAA
    CCCACAGACA CAAGGAGCCC CTGCACGGAT CCCCACAAGG CATTTGTTTA TGACACGGAT
    TCTCATGACG AGGCTGATGA GGACGCTTCC TCCTTAGCCT CTGTCCAAGC GGTTCCAAAC
    CAGGGCTGCA GGCTGCACAG TTGTAAATTA ACCCACAGTG TCTCTGACAG CCTCTTTGGC
    TGGGAGCTGA ATGGCAAACA CTGTTCAGAA GTCACTTCCT CGGTCTACTC CAGGGAGAGG
    CCTGAGAAGC TGACCGGTTG TGCCAGCAAC TGTCCCTTGG AGCAGAAGCT GTGCCCAGGC
    ATGCAGGGGC CTCGGGTACA GAGAGAGAAG GTCTCACCCA GCCAGGGCTG CCAGGCCCTC
    AGTCTCCAGC CCTCAGACAC CGATGACCCG GAGACCCTGG ATGAGCTGCA CATCGAGAGC
    AGTGATGAGA AAAGCCCTTC AGATGTGTCT GCAACCGCGG ACACTGACCA GTCCACGGAG
    AGCCTGGATA TCCTCACAGG ATTTGAAAAA TCTTCATGTG GGTCTCCCGA GGAGGAAGAA
    AACCAAGTAC CCGTCCATTT AGAGAGCAGG CCAAAGACGT TTAGTTTCAT TAAGCAACAG
    AGGGTTGTAA AACGGACTTC CTCAGAAGAA TGTATTACTG TGATATTTGA TGCAGAAGAC
    GGCGAGCCCA TTGAGTTCAG CTCTCACCAG ACTGGCCTTG TTACTGTTAC CAGAAAGGAG
    ATTTCCATCC ATCAGGCCCC TGCTGGGCCC CAGATGGAAC ACACTGAACT TTCACCTCAG
    GGAATTGCTC ATTTACAGCC AGGGGCTGCA GCTAGAGACT ACCCTTTTTT AAAGAGGTCT
    GAAGAGGAAA CTGAAAGAAA CATTCCACGA GATGAGGTAG ATGATCCTGC CATGACACCT
    GCTGTGTCTT TCCACCCCAG GACAGTGACG CAAAATACTC AAAGACTGGC CAAACCGACA
    CATGTCACAC CATGCCAGAG TCATTCTCGG TCATCTGTGG GCACAGGCGT CTGTCAGAAG
    AAAAGTCTGA CAAAAATACC TACCAGGGGC AAGTCTTCAC CTCAGAAATC GAGAGTAAGG
    GAGCCTGAAG CCTCCCTTAT GATGCCCTCA GCTGGCTCAG TGACTCTTGA GAAATCACCA
    GCTCCAGGGA AACTCTCACA GTTCAAAAAG ACTGAAGGTC CAGCTCCCCT TTTTGATTTG
    CAGCCAGATT CACACATTCC AAAACCCCCC ACCCAGCTTC CTCATGGCTC CAAGATGTCC
    AGCAGAAGGG ATTGGGTCCA GAGCTCCAAG AATCAGATGC TAGCATCACA GTTGCTGTCC
    AGGCCCCCCA CTGAGCGGAG TGACGATGGG GAGCCCCCCA CGAGGGATAA ACACTGTGAT
    CCAGGGCCAG AGGCCGGGGT GAAATCGCCC TCCCCACCAT CCCCTCCAGG CAGGTCGGTC
    TCCCTACTCA TCAGGCCCAG TTATGACTTT TTGCCACCAC CTTCATCTGC CAAATCCGAA
    AGCAGGGTCT CCACCGAGAC AGCAAGAATG GTATTGAAAT CACCCCCTCT GAAAGGATCC
    TCTGCTCCTG TTATTTATTT TAACCAGACC CTGACAGATG TGCAGGGGAA GAAACCGTCT
    GTGGCCTTCA AAAAGCCCAT CTTCACTCCC TCACCACCCT CCGCAGAAAC AGCAATCCAG
    ATGAGATGCC CTGCTCACAG CCCCTCCAGC TCATTTGCTG TGATGGTCCC GGAGCCCCCA
    AAAGTCTCTC CAAAGAGAAA TGCCCCCAGA GCCCCACCTC ACCAAACACT TGGGACCACA
    CAGAACAACA CAGGGTTACA GACTCCCAAG AACTATGCTT CCCCCCGTGA GCCACTGGAA
    ATACCATCCT CCAAAGGTGT ATCTCCAGGG AGAAAAGAAA AGTTGAGTGG CAGTGTCTCT
    GCATCTTCCA AGCCTTCCTT CTTAGGGGTA AATGAGTCAC CATCATCTCA GGTTAACAGT
    CCCTTATCAT CAGCATCCTT CAAAAGCCAT AACCCCCTAC ATGGTTGTCA AAACCTTCAT
    GAGAGAGGAC TGAAAACTCG CTTCCCAGTT GGGCTCAAAG TTTTCATGAA GTCTCCCCAG
    TTGCTCAGGA AAAGTTCCAC GGTGCCAGGG AAACAGGAAA AAGACAGTCT AAATGAAGCC
    TCGAAAACCT CTGTGGCTGT GAGCAAGGCT AAGCCAGGGG CCTCCAGAAA TCCAGCCAGC
    CTTGAGACCA CAGGAGGGGA AAGAAATACG TCTCCAGTGA GTTTACCAGC ACAAGAGAGT
    TTGGCCAAAG GGCTTCCCCT GGAAAGAGCA ATGCCAGAGT CACTGGAAAA TAGCATGCCT
    GGGGCTGATA GAAAAGATGG AGTAGAAAAC AGGTCTGTAA AAAGATCCCT GTCCTCCAGC
    AAACCACACC TGAAACCAGC TCTAGGCATG AACGGAGCCA AAGCCCGCAG CCAGAGTTTC
    AGTGCTCACT CAGGGGAAAA GCCCCCCACG CCCCCCACAG AAGGGCTGGG CAAAGTCCGG
    ACTCAAATTA TTACCAACAC CGCTGAGAGA GGCAATTCCC TCACCCGGCA GAGCTCTTCT
    GCCGAAGGCT CTCCCAGCAA GAGTGCGTCT ACCCCTGTGT CTGATGGTCT CCCCAGCACT
    GGGAAGCCCC TGGGACACCC TTCCCCCAGG CAGGGTTCCT TGGGGAGCAC AGGCAGCTCC
    AGCAGCCAGC ATGGGAGCCC AAGCAAGTTG CCTTTGAGGA TCCCTCCAAA ATCCGAGGAG
    CCCCTCACCC CAGCTGGAAC AGAAGAGCAG CAGGCCTATG CCCAGGGAGA AGGCCCCAGG
    GTGACGGTAC CTGAGGAACC AGGCAGCGAC CACTGCAGGT GCCCACCTAC CCCAACAGAT
    AGCTCAGGAG GCCCACAGAG CCTGGGGAGG ACGCCACATC CCAGCAGTTT CACAGCAAGC
    AGGACCTCCA AGCTAGAAAC TTCCGGAAGG TATCCAGATA CTTCTACAAC CAGGGCCGGT
    GTCGTAAGCC CAGAAGCCCC GCTCTCACCC ACAATTGAAG AAAAGGTCAT GTTGTGCATT
    CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGTCCA CCTCTGTGGA AGCAAAACCG
    AAGCCGGGGC CTTCTTTTGC CAGCTGGTTC GGTTTTCGGA GGAGCAGACT CCCGGCTCTC
    AGTAGCAGGA AGATGGATGT CTCCAGAACC AAAGCAGAAA AGAAAGATGC AAAAGGTTTA
    GGGTTTGGAA ACAAACAGCT GAAATCAGAG AGAAAGAAAG AGAAAAAGAA GCCTGAGCTA
    CAGTGTGAGA TGGAAAATGA ACTTCACAGG GATATCGAGT TGGCAGATGG TCCAGACAGT
    GGTTTGCAAA ACAGAAATAA CCCGAAAACA CCGCCAGACA TTTATGATCA AGTGAAGTTT
    GAATCAAGAA ATAGACCCAG CCCTGTTCCA TGTTCAGCGA AAGATACCTT TATGACAGAA
    CTTCTGAACA GAGTTGATAA GAAAGCAGCT CAACAGACAG AAAGTGGATC AAATAGTCTC
    TCCTGTAGGA GTGTGTTAAA GGGCAGCTCC CAGGGCTCCT GTCTCACCAG CAGCTCTCTC
    AGCACTCAAG GAAACCACAA GAAAAACATC AAAACCAAAG CAGATATGGA AAAACCGAAA
    GGCTCCCTGG TAAGAGAGGC AAATGATCAC CTGCAAGAGG ATGAAGAAGA TACAGTCGCA
    GATTCTGCAT TTCAGAGCCA TACCATAGAA ACAAACTGCC AGATGAGGAC CCTGGACAGT
    GGGATTGGAA CCTTCCCACT CCCAGACTCA GGAACTCGTG CAGCGGGACG ATACATACGC
    CAAGCAGACT CCCCTGAGGA CACTGACCCC ATCCTGTCTC TCCAACCGGC CCTTTGTGCA
    GCCTCTTCCA TCAGAGCCCA GACCCTTGAA AGAGAAGTGC CTTCCTCTGC AGATAGCCAG
    GGCTCTGCAG ACAATGCCAT TGTTCACTCC ACATCCGACC CCATCATGAC TGCCAGAGGG
    ACGCGACCTC TTCAGAGTCT CCTCCCCAAA CCAGCCTCCT CAGGAAAAAT CAATTCCCAA
    ATGAAGAGTG AAGCCGAGCC AAGGTCTCAA AATTGTTCAC CATTCCAATA TGTCGAAAAC
    ACAATGGCGA GCAAGCCACT TCCAGCCTGG GAGGGTGATG ACGCTGCTGC AGAGACCCAG
    AAATTGAAAC AAGTTGAAGA AACCAGAGAA GATCCTGAGA ATAGATTATG CAAAATTTCC
    CTGGAGTCAT TCAATAAATA TGATGGCAAT ACTGTGATCT TACTAGGAAA GGAGAAGAAC
    TCTCTGAACA AGGTTGAAGG ACAAAAGGAA GAAAAAGAAA AAACTGAAGA GGCCTCTTTG
    AGTAGCTCTG AGAGACCTGG GGTAGACCAC CTGGAATCTT TGAGTGATTC TTTATATGAT
    AGCTTCTCTT CCTGTGCAAG TCAGGGTTCA AATGATGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331333.1
    CDS22..5283
    Translation

    Target ORF information:

    RefSeq Version XM_015475847.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475847.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGCTAG ATGAGGACTC GAGGAGTGAA GGGAGCAGTG CAGATGAGGG GAAAGGCAAG 
    ACCAAATGGC TGTTAGAAAG ATTGAAAGCT CTAGAGGCAG AGAATTCAGC ATTGGCTTTG
    GAAAATGAAA ATCAAAGGGA ACAGTATGAG AGATGTCTTG ATGAGGTAGC CAACCAGGTT
    GTGCAAGCCC TGCTCACTCA AAAGGATCTC AGGGAGGAAT GTGTAAAGTT GAAAACAAGA
    GTGTTTGATT TGGAACAACA GAATCGAACG CTAAGCATCC TTTTCCAACA GCGAGTCAGA
    CCAACTTCTG ATCTCCTCCT CCAGGGCTGC CAGCTGAACA CAAAAGCAGG AACCCCTGCT
    TTGAAATGCC CTGGCACGGG GATTGCCGTC TCTGGTCATC TCTGCCCTCG AAGCAGCTAC
    AGCAGCAGTG AGCTGTCTCT TTCCAGCACC TGCAGCGAGT ATTCCAGCAG CTCCTCCTAC
    ACGTGGCATG ATGGAAAGAA CCTCAGGAAA AGGCAGTCAT CACAAAATTG GGACAAAAGG
    TTAAGTATTG ACTCTTCGCT CCCGAGTGGC TTTGCTAGTC CTATGGATGA ACTACCTCCA
    ACTCGCATCA AGGAAAGCCA CATTTTGGAA GGACTAAGAA AGCTCCAGAA GCGAAAAGTG
    TTACTTGAAC CCCCATCAGT GATAACCAAA TGGGGTTATA AAGATTGCAT GAACTCCAAT
    GAAGGAATCT ATTCTCCAGG AATTAATAGT AACAGCCTGA AGGAATATCC ACCCTGCAAA
    CCCACAGACA CAAGGAGCCC CTGCACGGAT CCCCACAAGG CATTTGTTTA TGACACGGAT
    TCTCATGACG AGGCTGATGA GGACGCTTCC TCCTTAGCCT CTGTCCAAGC GGTTCCAAAC
    CAGGGCTGCA GGCTGCACAG TTGTAAATTA ACCCACAGTG TCTCTGACAG CCTCTTTGGC
    TGGGAGCTGA ATGGCAAACA CTGTTCAGAA GTCACTTCCT CGGTCTACTC CAGGGAGAGG
    CCTGAGAAGC TGACCGGTTG TGCCAGCAAC TGTCCCTTGG AGCAGAAGCT GTGCCCAGGC
    ATGCAGGGGC CTCGGGTACA GAGAGAGAAG GTCTCACCCA GCCAGGGCTG CCAGGCCCTC
    AGTCTCCAGC CCTCAGACAC CGATGACCCG GAGACCCTGG ATGAGCTGCA CATCGAGAGC
    AGTGATGAGA AAAGCCCTTC AGATGTGTCT GCAACCGCGG ACACTGACCA GTCCACGGAG
    AGCCTGGATA TCCTCACAGG ATTTGAAAAA TCTTCATGTG GGTCTCCCGA GGAGGAAGAA
    AACCAAGTAC CCGTCCATTT AGAGAGCAGG CCAAAGACGT TTAGTTTCAT TAAGCAACAG
    AGGGTTGTAA AACGGACTTC CTCAGAAGAA TGTATTACTG TGATATTTGA TGCAGAAGAC
    GGCGAGCCCA TTGAGTTCAG CTCTCACCAG ACTGGCCTTG TTACTGTTAC CAGAAAGGAG
    ATTTCCATCC ATCAGGCCCC TGCTGGGCCC CAGATGGAAC ACACTGAACT TTCACCTCAG
    GGAATTGCTC ATTTACAGCC AGGGGCTGCA GCTAGAGACT ACCCTTTTTT AAAGAGGTCT
    GAAGAGGAAA CTGAAAGAAA CATTCCACGA GATGAGGTAG ATGATCCTGC CATGACACCT
    GCTGTGTCTT TCCACCCCAG GACAGTGACG CAAAATACTC AAAGACTGGC CAAACCGACA
    CATGTCACAC CATGCCAGAG TCATTCTCGG TCATCTGTGG GCACAGGCGT CTGTCAGAAG
    AAAAGTCTGA CAAAAATACC TACCAGGGGC AAGTCTTCAC CTCAGAAATC GAGAGTAAGG
    GAGCCTGAAG CCTCCCTTAT GATGCCCTCA GCTGGCTCAG TGACTCTTGA GAAATCACCA
    GCTCCAGGGA AACTCTCACA GTTCAAAAAG ACTGAAGGTC CAGCTCCCCT TTTTGATTTG
    CAGCCAGATT CACACATTCC AAAACCCCCC ACCCAGCTTC CTCATGGCTC CAAGATGTCC
    AGCAGAAGGG ATTGGGTCCA GAGCTCCAAG AATCAGATGC TAGCATCACA GTTGCTGTCC
    AGGCCCCCCA CTGAGCGGAG TGACGATGGG GAGCCCCCCA CGAGGGATAA ACACTGTGAT
    CCAGGGCCAG AGGCCGGGGT GAAATCGCCC TCCCCACCAT CCCCTCCAGG CAGGTCGGTC
    TCCCTACTCA TCAGGCCCAG TTATGACTTT TTGCCACCAC CTTCATCTGC CAAATCCGAA
    AGCAGGGTCT CCACCGAGAC AGCAAGAATG GTATTGAAAT CACCCCCTCT GAAAGGATCC
    TCTGCTCCTG TTATTTATTT TAACCAGACC CTGACAGATG TGCAGGGGAA GAAACCGTCT
    GTGGCCTTCA AAAAGCCCAT CTTCACTCCC TCACCACCCT CCGCAGAAAC AGCAATCCAG
    ATGAGATGCC CTGCTCACAG CCCCTCCAGC TCATTTGCTG TGATGGTCCC GGAGCCCCCA
    AAAGTCTCTC CAAAGAGAAA TGCCCCCAGA GCCCCACCTC ACCAAACACT TGGGACCACA
    CAGAACAACA CAGGGTTACA GACTCCCAAG AACTATGCTT CCCCCCGTGA GCCACTGGAA
    ATACCATCCT CCAAAGGTGT ATCTCCAGGG AGAAAAGAAA AGTTGAGTGG CAGTGTCTCT
    GCATCTTCCA AGCCTTCCTT CTTAGGGGTA AATGAGTCAC CATCATCTCA GGTTAACAGT
    CCCTTATCAT CAGCATCCTT CAAAAGCCAT AACCCCCTAC ATGGTTGTCA AAACCTTCAT
    GAGAGAGGAC TGAAAACTCG CTTCCCAGTT GGGCTCAAAG TTTTCATGAA GTCTCCCCAG
    TTGCTCAGGA AAAGTTCCAC GGTGCCAGGG AAACAGGAAA AAGACAGTCT AAATGAAGCC
    TCGAAAACCT CTGTGGCTGT GAGCAAGGCT AAGCCAGGGG CCTCCAGAAA TCCAGCCAGC
    CTTGAGACCA CAGGAGGGGA AAGAAATACG TCTCCAGTGA GTTTACCAGC ACAAGAGAGT
    TTGGCCAAAG GGCTTCCCCT GGAAAGAGCA ATGCCAGAGT CACTGGAAAA TAGCATGCCT
    GGGGCTGATA GAAAAGATGG AGTAGAAAAC AGGTCTGTAA AAAGATCCCT GTCCTCCAGC
    AAACCACACC TGAAACCAGC TCTAGGCATG AACGGAGCCA AAGCCCGCAG CCAGAGTTTC
    AGTGCTCACT CAGGGGAAAA GCCCCCCACG CCCCCCACAG AAGGGCTGGG CAAAGTCCGG
    ACTCAAATTA TTACCAACAC CGCTGAGAGA GGCAATTCCC TCACCCGGCA GAGCTCTTCT
    GCCGAAGGCT CTCCCAGCAA GAGTGCGTCT ACCCCTGTGT CTGATGGTCT CCCCAGCACT
    GGGAAGCCCC TGGGACACCC TTCCCCCAGG CAGGGTTCCT TGGGGAGCAC AGGCAGCTCC
    AGCAGCCAGC ATGGGAGCCC AAGCAAGTTG CCTTTGAGGA TCCCTCCAAA ATCCGAGGAG
    CCCCTCACCC CAGCTGGAAC AGAAGAGCAG CAGGCCTATG CCCAGGGAGA AGGCCCCAGG
    GTGACGGTAC CTGAGGAACC AGGCAGCGAC CACTGCAGGT GCCCACCTAC CCCAACAGAT
    AGCTCAGGAG GCCCACAGAG CCTGGGGAGG ACGCCACATC CCAGCAGTTT CACAGCAAGC
    AGGACCTCCA AGCTAGAAAC TTCCGGAAGG TATCCAGATA CTTCTACAAC CAGGGCCGGT
    GTCGTAAGCC CAGAAGCCCC GCTCTCACCC ACAATTGAAG AAAAGGTCAT GTTGTGCATT
    CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGTCCA CCTCTGTGGA AGCAAAACCG
    AAGCCGGGGC CTTCTTTTGC CAGCTGGTTC GGTTTTCGGA GGAGCAGACT CCCGGCTCTC
    AGTAGCAGGA AGATGGATGT CTCCAGAACC AAAGCAGAAA AGAAAGATGC AAAAGGTTTA
    GGGTTTGGAA ACAAACAGCT GAAATCAGAG AGAAAGAAAG AGAAAAAGAA GCCTGAGCTA
    CAGTGTGAGA TGGAAAATGA ACTTCACAGG GATATCGAGT TGGCAGATGG TCCAGACAGT
    GGTTTGCAAA ACAGAAATAA CCCGAAAACA CCGCCAGACA TTTATGATCA AGTGAAGTTT
    GAATCAAGAA ATAGACCCAG CCCTGTTCCA TGTTCAGCGA AAGATACCTT TATGACAGAA
    CTTCTGAACA GAGTTGATAA GAAAGCAGCT CAACAGACAG AAAGTGGATC AAATAGTCTC
    TCCTGTAGGA GTGTGTTAAA GGGCAGCTCC CAGGGCTCCT GTCTCACCAG CAGCTCTCTC
    AGCACTCAAG GAAACCACAA GAAAAACATC AAAACCAAAG CAGATATGGA AAAACCGAAA
    GGCTCCCTGG TAAGAGAGGC AAATGATCAC CTGCAAGAGG ATGAAGAAGA TACAGTCGCA
    GATTCTGCAT TTCAGAGCCA TACCATAGAA ACAAACTGCC AGATGAGGAC CCTGGACAGT
    GGGATTGGAA CCTTCCCACT CCCAGACTCA GGAACTCGTG CAGCGGGACG ATACATACGC
    CAAGCAGACT CCCCTGAGGA CACTGACCCC ATCCTGTCTC TCCAACCGGC CCTTTGTGCA
    GCCTCTTCCA TCAGAGCCCA GACCCTTGAA AGAGAAGTGC CTTCCTCTGC AGATAGCCAG
    GGCTCTGCAG ACAATGCCAT TGTTCACTCC ACATCCGACC CCATCATGAC TGCCAGAGGG
    ACGCGACCTC TTCAGAGTCT CCTCCCCAAA CCAGCCTCCT CAGGAAAAAT CAATTCCCAA
    ATGAAGAGTG AAGCCGAGCC AAGGTCTCAA AATTGTTCAC CATTCCAATA TGTCGAAAAC
    ACAATGGCGA GCAAGCCACT TCCAGCCTGG GAGGGTGATG ACGCTGCTGC AGAGACCCAG
    AAATTGAAAC AAGTTGAAGA AACCAGAGAA GATCCTGAGA ATAGATTATG CAAAATTTCC
    CTGGAGTCAT TCAATAAATA TGATGGCAAT ACTGTGATCT TACTAGGAAA GGAGAAGAAC
    TCTCTGAACA AGGTTGAAGG ACAAAAGGAA GAAAAAGAAA AAACTGAAGA GGCCTCTTTG
    AGTAGCTCTG AGAGACCTGG GGTAGACCAC CTGGAATCTT TGAGTGATTC TTTATATGAT
    AGCTTCTCTT CCTGTGCAAG TCAGGGTTCA AATGATGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87935
    Clone ID Related Accession (Same CDS sequence) XM_015475847.1 , XM_015462321.1
    Accession Version XM_015462321.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nck-associated protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644597.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGCTAG ATGAGGACTC GAGGAGTGAA GGGAGCAGTG CAGATGAGGG GAAAGGCAAG 
    ACCAAATGGC TGTTAGAAAG ATTGAAAGCT CTAGAGGCAG AGAATTCAGC ATTGGCTTTG
    GAAAATGAAA ATCAAAGGGA ACAGTATGAG AGATGTCTTG ATGAGGTAGC CAACCAGGTT
    GTGCAAGCCC TGCTCACTCA AAAGGATCTC AGGGAGGAAT GTGTAAAGTT GAAAACAAGA
    GTGTTTGATT TGGAACAACA GAATCGAACG CTAAGCATCC TTTTCCAACA GCGAGTCAGA
    CCAACTTCTG ATCTCCTCCT CCAGGGCTGC CAGCTGAACA CAAAAGCAGG AACCCCTGCT
    TTGAAATGCC CTGGCACGGG GATTGCCGTC TCTGGTCATC TCTGCCCTCG AAGCAGCTAC
    AGCAGCAGTG AGCTGTCTCT TTCCAGCACC TGCAGCGAGT ATTCCAGCAG CTCCTCCTAC
    ACGTGGCATG ATGGAAAGAA CCTCAGGAAA AGGCAGTCAT CACAAAATTG GGACAAAAGG
    TTAAGTATTG ACTCTTCGCT CCCGAGTGGC TTTGCTAGTC CTATGGATGA ACTACCTCCA
    ACTCGCATCA AGGAAAGCCA CATTTTGGAA GGACTAAGAA AGCTCCAGAA GCGAAAAGTG
    TTACTTGAAC CCCCATCAGT GATAACCAAA TGGGGTTATA AAGATTGCAT GAACTCCAAT
    GAAGGAATCT ATTCTCCAGG AATTAATAGT AACAGCCTGA AGGAATATCC ACCCTGCAAA
    CCCACAGACA CAAGGAGCCC CTGCACGGAT CCCCACAAGG CATTTGTTTA TGACACGGAT
    TCTCATGACG AGGCTGATGA GGACGCTTCC TCCTTAGCCT CTGTCCAAGC GGTTCCAAAC
    CAGGGCTGCA GGCTGCACAG TTGTAAATTA ACCCACAGTG TCTCTGACAG CCTCTTTGGC
    TGGGAGCTGA ATGGCAAACA CTGTTCAGAA GTCACTTCCT CGGTCTACTC CAGGGAGAGG
    CCTGAGAAGC TGACCGGTTG TGCCAGCAAC TGTCCCTTGG AGCAGAAGCT GTGCCCAGGC
    ATGCAGGGGC CTCGGGTACA GAGAGAGAAG GTCTCACCCA GCCAGGGCTG CCAGGCCCTC
    AGTCTCCAGC CCTCAGACAC CGATGACCCG GAGACCCTGG ATGAGCTGCA CATCGAGAGC
    AGTGATGAGA AAAGCCCTTC AGATGTGTCT GCAACCGCGG ACACTGACCA GTCCACGGAG
    AGCCTGGATA TCCTCACAGG ATTTGAAAAA TCTTCATGTG GGTCTCCCGA GGAGGAAGAA
    AACCAAGTAC CCGTCCATTT AGAGAGCAGG CCAAAGACGT TTAGTTTCAT TAAGCAACAG
    AGGGTTGTAA AACGGACTTC CTCAGAAGAA TGTATTACTG TGATATTTGA TGCAGAAGAC
    GGCGAGCCCA TTGAGTTCAG CTCTCACCAG ACTGGCCTTG TTACTGTTAC CAGAAAGGAG
    ATTTCCATCC ATCAGGCCCC TGCTGGGCCC CAGATGGAAC ACACTGAACT TTCACCTCAG
    GGAATTGCTC ATTTACAGCC AGGGGCTGCA GCTAGAGACT ACCCTTTTTT AAAGAGGTCT
    GAAGAGGAAA CTGAAAGAAA CATTCCACGA GATGAGGTAG ATGATCCTGC CATGACACCT
    GCTGTGTCTT TCCACCCCAG GACAGTGACG CAAAATACTC AAAGACTGGC CAAACCGACA
    CATGTCACAC CATGCCAGAG TCATTCTCGG TCATCTGTGG GCACAGGCGT CTGTCAGAAG
    AAAAGTCTGA CAAAAATACC TACCAGGGGC AAGTCTTCAC CTCAGAAATC GAGAGTAAGG
    GAGCCTGAAG CCTCCCTTAT GATGCCCTCA GCTGGCTCAG TGACTCTTGA GAAATCACCA
    GCTCCAGGGA AACTCTCACA GTTCAAAAAG ACTGAAGGTC CAGCTCCCCT TTTTGATTTG
    CAGCCAGATT CACACATTCC AAAACCCCCC ACCCAGCTTC CTCATGGCTC CAAGATGTCC
    AGCAGAAGGG ATTGGGTCCA GAGCTCCAAG AATCAGATGC TAGCATCACA GTTGCTGTCC
    AGGCCCCCCA CTGAGCGGAG TGACGATGGG GAGCCCCCCA CGAGGGATAA ACACTGTGAT
    CCAGGGCCAG AGGCCGGGGT GAAATCGCCC TCCCCACCAT CCCCTCCAGG CAGGTCGGTC
    TCCCTACTCA TCAGGCCCAG TTATGACTTT TTGCCACCAC CTTCATCTGC CAAATCCGAA
    AGCAGGGTCT CCACCGAGAC AGCAAGAATG GTATTGAAAT CACCCCCTCT GAAAGGATCC
    TCTGCTCCTG TTATTTATTT TAACCAGACC CTGACAGATG TGCAGGGGAA GAAACCGTCT
    GTGGCCTTCA AAAAGCCCAT CTTCACTCCC TCACCACCCT CCGCAGAAAC AGCAATCCAG
    ATGAGATGCC CTGCTCACAG CCCCTCCAGC TCATTTGCTG TGATGGTCCC GGAGCCCCCA
    AAAGTCTCTC CAAAGAGAAA TGCCCCCAGA GCCCCACCTC ACCAAACACT TGGGACCACA
    CAGAACAACA CAGGGTTACA GACTCCCAAG AACTATGCTT CCCCCCGTGA GCCACTGGAA
    ATACCATCCT CCAAAGGTGT ATCTCCAGGG AGAAAAGAAA AGTTGAGTGG CAGTGTCTCT
    GCATCTTCCA AGCCTTCCTT CTTAGGGGTA AATGAGTCAC CATCATCTCA GGTTAACAGT
    CCCTTATCAT CAGCATCCTT CAAAAGCCAT AACCCCCTAC ATGGTTGTCA AAACCTTCAT
    GAGAGAGGAC TGAAAACTCG CTTCCCAGTT GGGCTCAAAG TTTTCATGAA GTCTCCCCAG
    TTGCTCAGGA AAAGTTCCAC GGTGCCAGGG AAACAGGAAA AAGACAGTCT AAATGAAGCC
    TCGAAAACCT CTGTGGCTGT GAGCAAGGCT AAGCCAGGGG CCTCCAGAAA TCCAGCCAGC
    CTTGAGACCA CAGGAGGGGA AAGAAATACG TCTCCAGTGA GTTTACCAGC ACAAGAGAGT
    TTGGCCAAAG GGCTTCCCCT GGAAAGAGCA ATGCCAGAGT CACTGGAAAA TAGCATGCCT
    GGGGCTGATA GAAAAGATGG AGTAGAAAAC AGGTCTGTAA AAAGATCCCT GTCCTCCAGC
    AAACCACACC TGAAACCAGC TCTAGGCATG AACGGAGCCA AAGCCCGCAG CCAGAGTTTC
    AGTGCTCACT CAGGGGAAAA GCCCCCCACG CCCCCCACAG AAGGGCTGGG CAAAGTCCGG
    ACTCAAATTA TTACCAACAC CGCTGAGAGA GGCAATTCCC TCACCCGGCA GAGCTCTTCT
    GCCGAAGGCT CTCCCAGCAA GAGTGCGTCT ACCCCTGTGT CTGATGGTCT CCCCAGCACT
    GGGAAGCCCC TGGGACACCC TTCCCCCAGG CAGGGTTCCT TGGGGAGCAC AGGCAGCTCC
    AGCAGCCAGC ATGGGAGCCC AAGCAAGTTG CCTTTGAGGA TCCCTCCAAA ATCCGAGGAG
    CCCCTCACCC CAGCTGGAAC AGAAGAGCAG CAGGCCTATG CCCAGGGAGA AGGCCCCAGG
    GTGACGGTAC CTGAGGAACC AGGCAGCGAC CACTGCAGGT GCCCACCTAC CCCAACAGAT
    AGCTCAGGAG GCCCACAGAG CCTGGGGAGG ACGCCACATC CCAGCAGTTT CACAGCAAGC
    AGGACCTCCA AGCTAGAAAC TTCCGGAAGG TATCCAGATA CTTCTACAAC CAGGGCCGGT
    GTCGTAAGCC CAGAAGCCCC GCTCTCACCC ACAATTGAAG AAAAGGTCAT GTTGTGCATT
    CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGTCCA CCTCTGTGGA AGCAAAACCG
    AAGCCGGGGC CTTCTTTTGC CAGCTGGTTC GGTTTTCGGA GGAGCAGACT CCCGGCTCTC
    AGTAGCAGGA AGATGGATGT CTCCAGAACC AAAGCAGAAA AGAAAGATGC AAAAGGTTTA
    GGGTTTGGAA ACAAACAGCT GAAATCAGAG AGAAAGAAAG AGAAAAAGAA GCCTGAGCTA
    CAGTGTGAGA TGGAAAATGA ACTTCACAGG GATATCGAGT TGGCAGATGG TCCAGACAGT
    GGTTTGCAAA ACAGAAATAA CCCGAAAACA CCGCCAGACA TTTATGATCA AGTGAAGTTT
    GAATCAAGAA ATAGACCCAG CCCTGTTCCA TGTTCAGCGA AAGATACCTT TATGACAGAA
    CTTCTGAACA GAGTTGATAA GAAAGCAGCT CAACAGACAG AAAGTGGATC AAATAGTCTC
    TCCTGTAGGA GTGTGTTAAA GGGCAGCTCC CAGGGCTCCT GTCTCACCAG CAGCTCTCTC
    AGCACTCAAG GAAACCACAA GAAAAACATC AAAACCAAAG CAGATATGGA AAAACCGAAA
    GGCTCCCTGG TAAGAGAGGC AAATGATCAC CTGCAAGAGG ATGAAGAAGA TACAGTCGCA
    GATTCTGCAT TTCAGAGCCA TACCATAGAA ACAAACTGCC AGATGAGGAC CCTGGACAGT
    GGGATTGGAA CCTTCCCACT CCCAGACTCA GGAACTCGTG CAGCGGGACG ATACATACGC
    CAAGCAGACT CCCCTGAGGA CACTGACCCC ATCCTGTCTC TCCAACCGGC CCTTTGTGCA
    GCCTCTTCCA TCAGAGCCCA GACCCTTGAA AGAGAAGTGC CTTCCTCTGC AGATAGCCAG
    GGCTCTGCAG ACAATGCCAT TGTTCACTCC ACATCCGACC CCATCATGAC TGCCAGAGGG
    ACGCGACCTC TTCAGAGTCT CCTCCCCAAA CCAGCCTCCT CAGGAAAAAT CAATTCCCAA
    ATGAAGAGTG AAGCCGAGCC AAGGTCTCAA AATTGTTCAC CATTCCAATA TGTCGAAAAC
    ACAATGGCGA GCAAGCCACT TCCAGCCTGG GAGGGTGATG ACGCTGCTGC AGAGACCCAG
    AAATTGAAAC AAGTTGAAGA AACCAGAGAA GATCCTGAGA ATAGATTATG CAAAATTTCC
    CTGGAGTCAT TCAATAAATA TGATGGCAAT ACTGTGATCT TACTAGGAAA GGAGAAGAAC
    TCTCTGAACA AGGTTGAAGG ACAAAAGGAA GAAAAAGAAA AAACTGAAGA GGCCTCTTTG
    AGTAGCTCTG AGAGACCTGG GGTAGACCAC CTGGAATCTT TGAGTGATTC TTTATATGAT
    AGCTTCTCTT CCTGTGCAAG TCAGGGTTCA AATGATGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317807.1
    CDS22..5283
    Translation

    Target ORF information:

    RefSeq Version XM_015462321.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462321.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGCTAG ATGAGGACTC GAGGAGTGAA GGGAGCAGTG CAGATGAGGG GAAAGGCAAG 
    ACCAAATGGC TGTTAGAAAG ATTGAAAGCT CTAGAGGCAG AGAATTCAGC ATTGGCTTTG
    GAAAATGAAA ATCAAAGGGA ACAGTATGAG AGATGTCTTG ATGAGGTAGC CAACCAGGTT
    GTGCAAGCCC TGCTCACTCA AAAGGATCTC AGGGAGGAAT GTGTAAAGTT GAAAACAAGA
    GTGTTTGATT TGGAACAACA GAATCGAACG CTAAGCATCC TTTTCCAACA GCGAGTCAGA
    CCAACTTCTG ATCTCCTCCT CCAGGGCTGC CAGCTGAACA CAAAAGCAGG AACCCCTGCT
    TTGAAATGCC CTGGCACGGG GATTGCCGTC TCTGGTCATC TCTGCCCTCG AAGCAGCTAC
    AGCAGCAGTG AGCTGTCTCT TTCCAGCACC TGCAGCGAGT ATTCCAGCAG CTCCTCCTAC
    ACGTGGCATG ATGGAAAGAA CCTCAGGAAA AGGCAGTCAT CACAAAATTG GGACAAAAGG
    TTAAGTATTG ACTCTTCGCT CCCGAGTGGC TTTGCTAGTC CTATGGATGA ACTACCTCCA
    ACTCGCATCA AGGAAAGCCA CATTTTGGAA GGACTAAGAA AGCTCCAGAA GCGAAAAGTG
    TTACTTGAAC CCCCATCAGT GATAACCAAA TGGGGTTATA AAGATTGCAT GAACTCCAAT
    GAAGGAATCT ATTCTCCAGG AATTAATAGT AACAGCCTGA AGGAATATCC ACCCTGCAAA
    CCCACAGACA CAAGGAGCCC CTGCACGGAT CCCCACAAGG CATTTGTTTA TGACACGGAT
    TCTCATGACG AGGCTGATGA GGACGCTTCC TCCTTAGCCT CTGTCCAAGC GGTTCCAAAC
    CAGGGCTGCA GGCTGCACAG TTGTAAATTA ACCCACAGTG TCTCTGACAG CCTCTTTGGC
    TGGGAGCTGA ATGGCAAACA CTGTTCAGAA GTCACTTCCT CGGTCTACTC CAGGGAGAGG
    CCTGAGAAGC TGACCGGTTG TGCCAGCAAC TGTCCCTTGG AGCAGAAGCT GTGCCCAGGC
    ATGCAGGGGC CTCGGGTACA GAGAGAGAAG GTCTCACCCA GCCAGGGCTG CCAGGCCCTC
    AGTCTCCAGC CCTCAGACAC CGATGACCCG GAGACCCTGG ATGAGCTGCA CATCGAGAGC
    AGTGATGAGA AAAGCCCTTC AGATGTGTCT GCAACCGCGG ACACTGACCA GTCCACGGAG
    AGCCTGGATA TCCTCACAGG ATTTGAAAAA TCTTCATGTG GGTCTCCCGA GGAGGAAGAA
    AACCAAGTAC CCGTCCATTT AGAGAGCAGG CCAAAGACGT TTAGTTTCAT TAAGCAACAG
    AGGGTTGTAA AACGGACTTC CTCAGAAGAA TGTATTACTG TGATATTTGA TGCAGAAGAC
    GGCGAGCCCA TTGAGTTCAG CTCTCACCAG ACTGGCCTTG TTACTGTTAC CAGAAAGGAG
    ATTTCCATCC ATCAGGCCCC TGCTGGGCCC CAGATGGAAC ACACTGAACT TTCACCTCAG
    GGAATTGCTC ATTTACAGCC AGGGGCTGCA GCTAGAGACT ACCCTTTTTT AAAGAGGTCT
    GAAGAGGAAA CTGAAAGAAA CATTCCACGA GATGAGGTAG ATGATCCTGC CATGACACCT
    GCTGTGTCTT TCCACCCCAG GACAGTGACG CAAAATACTC AAAGACTGGC CAAACCGACA
    CATGTCACAC CATGCCAGAG TCATTCTCGG TCATCTGTGG GCACAGGCGT CTGTCAGAAG
    AAAAGTCTGA CAAAAATACC TACCAGGGGC AAGTCTTCAC CTCAGAAATC GAGAGTAAGG
    GAGCCTGAAG CCTCCCTTAT GATGCCCTCA GCTGGCTCAG TGACTCTTGA GAAATCACCA
    GCTCCAGGGA AACTCTCACA GTTCAAAAAG ACTGAAGGTC CAGCTCCCCT TTTTGATTTG
    CAGCCAGATT CACACATTCC AAAACCCCCC ACCCAGCTTC CTCATGGCTC CAAGATGTCC
    AGCAGAAGGG ATTGGGTCCA GAGCTCCAAG AATCAGATGC TAGCATCACA GTTGCTGTCC
    AGGCCCCCCA CTGAGCGGAG TGACGATGGG GAGCCCCCCA CGAGGGATAA ACACTGTGAT
    CCAGGGCCAG AGGCCGGGGT GAAATCGCCC TCCCCACCAT CCCCTCCAGG CAGGTCGGTC
    TCCCTACTCA TCAGGCCCAG TTATGACTTT TTGCCACCAC CTTCATCTGC CAAATCCGAA
    AGCAGGGTCT CCACCGAGAC AGCAAGAATG GTATTGAAAT CACCCCCTCT GAAAGGATCC
    TCTGCTCCTG TTATTTATTT TAACCAGACC CTGACAGATG TGCAGGGGAA GAAACCGTCT
    GTGGCCTTCA AAAAGCCCAT CTTCACTCCC TCACCACCCT CCGCAGAAAC AGCAATCCAG
    ATGAGATGCC CTGCTCACAG CCCCTCCAGC TCATTTGCTG TGATGGTCCC GGAGCCCCCA
    AAAGTCTCTC CAAAGAGAAA TGCCCCCAGA GCCCCACCTC ACCAAACACT TGGGACCACA
    CAGAACAACA CAGGGTTACA GACTCCCAAG AACTATGCTT CCCCCCGTGA GCCACTGGAA
    ATACCATCCT CCAAAGGTGT ATCTCCAGGG AGAAAAGAAA AGTTGAGTGG CAGTGTCTCT
    GCATCTTCCA AGCCTTCCTT CTTAGGGGTA AATGAGTCAC CATCATCTCA GGTTAACAGT
    CCCTTATCAT CAGCATCCTT CAAAAGCCAT AACCCCCTAC ATGGTTGTCA AAACCTTCAT
    GAGAGAGGAC TGAAAACTCG CTTCCCAGTT GGGCTCAAAG TTTTCATGAA GTCTCCCCAG
    TTGCTCAGGA AAAGTTCCAC GGTGCCAGGG AAACAGGAAA AAGACAGTCT AAATGAAGCC
    TCGAAAACCT CTGTGGCTGT GAGCAAGGCT AAGCCAGGGG CCTCCAGAAA TCCAGCCAGC
    CTTGAGACCA CAGGAGGGGA AAGAAATACG TCTCCAGTGA GTTTACCAGC ACAAGAGAGT
    TTGGCCAAAG GGCTTCCCCT GGAAAGAGCA ATGCCAGAGT CACTGGAAAA TAGCATGCCT
    GGGGCTGATA GAAAAGATGG AGTAGAAAAC AGGTCTGTAA AAAGATCCCT GTCCTCCAGC
    AAACCACACC TGAAACCAGC TCTAGGCATG AACGGAGCCA AAGCCCGCAG CCAGAGTTTC
    AGTGCTCACT CAGGGGAAAA GCCCCCCACG CCCCCCACAG AAGGGCTGGG CAAAGTCCGG
    ACTCAAATTA TTACCAACAC CGCTGAGAGA GGCAATTCCC TCACCCGGCA GAGCTCTTCT
    GCCGAAGGCT CTCCCAGCAA GAGTGCGTCT ACCCCTGTGT CTGATGGTCT CCCCAGCACT
    GGGAAGCCCC TGGGACACCC TTCCCCCAGG CAGGGTTCCT TGGGGAGCAC AGGCAGCTCC
    AGCAGCCAGC ATGGGAGCCC AAGCAAGTTG CCTTTGAGGA TCCCTCCAAA ATCCGAGGAG
    CCCCTCACCC CAGCTGGAAC AGAAGAGCAG CAGGCCTATG CCCAGGGAGA AGGCCCCAGG
    GTGACGGTAC CTGAGGAACC AGGCAGCGAC CACTGCAGGT GCCCACCTAC CCCAACAGAT
    AGCTCAGGAG GCCCACAGAG CCTGGGGAGG ACGCCACATC CCAGCAGTTT CACAGCAAGC
    AGGACCTCCA AGCTAGAAAC TTCCGGAAGG TATCCAGATA CTTCTACAAC CAGGGCCGGT
    GTCGTAAGCC CAGAAGCCCC GCTCTCACCC ACAATTGAAG AAAAGGTCAT GTTGTGCATT
    CAGGAAAATG TGGAAAAGGG CCAAGTGCAA ACAAAGTCCA CCTCTGTGGA AGCAAAACCG
    AAGCCGGGGC CTTCTTTTGC CAGCTGGTTC GGTTTTCGGA GGAGCAGACT CCCGGCTCTC
    AGTAGCAGGA AGATGGATGT CTCCAGAACC AAAGCAGAAA AGAAAGATGC AAAAGGTTTA
    GGGTTTGGAA ACAAACAGCT GAAATCAGAG AGAAAGAAAG AGAAAAAGAA GCCTGAGCTA
    CAGTGTGAGA TGGAAAATGA ACTTCACAGG GATATCGAGT TGGCAGATGG TCCAGACAGT
    GGTTTGCAAA ACAGAAATAA CCCGAAAACA CCGCCAGACA TTTATGATCA AGTGAAGTTT
    GAATCAAGAA ATAGACCCAG CCCTGTTCCA TGTTCAGCGA AAGATACCTT TATGACAGAA
    CTTCTGAACA GAGTTGATAA GAAAGCAGCT CAACAGACAG AAAGTGGATC AAATAGTCTC
    TCCTGTAGGA GTGTGTTAAA GGGCAGCTCC CAGGGCTCCT GTCTCACCAG CAGCTCTCTC
    AGCACTCAAG GAAACCACAA GAAAAACATC AAAACCAAAG CAGATATGGA AAAACCGAAA
    GGCTCCCTGG TAAGAGAGGC AAATGATCAC CTGCAAGAGG ATGAAGAAGA TACAGTCGCA
    GATTCTGCAT TTCAGAGCCA TACCATAGAA ACAAACTGCC AGATGAGGAC CCTGGACAGT
    GGGATTGGAA CCTTCCCACT CCCAGACTCA GGAACTCGTG CAGCGGGACG ATACATACGC
    CAAGCAGACT CCCCTGAGGA CACTGACCCC ATCCTGTCTC TCCAACCGGC CCTTTGTGCA
    GCCTCTTCCA TCAGAGCCCA GACCCTTGAA AGAGAAGTGC CTTCCTCTGC AGATAGCCAG
    GGCTCTGCAG ACAATGCCAT TGTTCACTCC ACATCCGACC CCATCATGAC TGCCAGAGGG
    ACGCGACCTC TTCAGAGTCT CCTCCCCAAA CCAGCCTCCT CAGGAAAAAT CAATTCCCAA
    ATGAAGAGTG AAGCCGAGCC AAGGTCTCAA AATTGTTCAC CATTCCAATA TGTCGAAAAC
    ACAATGGCGA GCAAGCCACT TCCAGCCTGG GAGGGTGATG ACGCTGCTGC AGAGACCCAG
    AAATTGAAAC AAGTTGAAGA AACCAGAGAA GATCCTGAGA ATAGATTATG CAAAATTTCC
    CTGGAGTCAT TCAATAAATA TGATGGCAAT ACTGTGATCT TACTAGGAAA GGAGAAGAAC
    TCTCTGAACA AGGTTGAAGG ACAAAAGGAA GAAAAAGAAA AAACTGAAGA GGCCTCTTTG
    AGTAGCTCTG AGAGACCTGG GGTAGACCAC CTGGAATCTT TGAGTGATTC TTTATATGAT
    AGCTTCTCTT CCTGTGCAAG TCAGGGTTCA AATGATGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16499
    Clone ID Related Accession (Same CDS sequence) XM_015462322.1 , XM_002685418.4
    Accession Version XM_015462322.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5715bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nck-associated protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644597.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAGGGCA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TTTAGATAGC 
    AGTCTCGTGG AATACATGGA CTCCAACAAA TACATTGAAC ATCTGTTAAA TCAACTTGAG
    GAGCAACACA GAAGTCTCTG GAGGGAGAAG TTGGCTGTGG CCCGACTGCA GAGAGAAGTT
    GCCCAGAGAA GAAGTGAAGG GGCCATGCAT GAGAAGCTGA TACATGAATT GGAAGAGGAG
    AGACACTTGC GTCTTCAGAG TGAGAAGCGC TTGCAGGAGG TGACACTAGA GTCTGAGCGC
    AACAGAATTC AGATGCGCGG CCTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA
    AATCTACTAC AGAGTCAAGG ACCTCCGGAA CAGAAAAAAG AAGAAACTGT TAATATAATG
    GTCTATCAGG AAAGAGGAAG CAGGGAGGAA CTGAAACACT GTGCGGCTTT GGAAGGCCGT
    CACATGGCGC TAGATGAGGA CTCGAGGAGT GAAGGGAGCA GTGCAGATGA GGGGAAAGGC
    AAGACCAAAT GGCTGTTAGA AAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCATTGGCT
    TTGGAAAATG AAAATCAAAG GGAACAGTAT GAGAGATGTC TTGATGAGGT AGCCAACCAG
    GTTGTGCAAG CCCTGCTCAC TCAAAAGGAT CTCAGGGAGG AATGTGTAAA GTTGAAAACA
    AGAGTGTTTG ATTTGGAACA ACAGAATCGA ACGCTAAGCA TCCTTTTCCA ACAGCGAGTC
    AGACCAACTT CTGATCTCCT CCTCCAGAAA CTTCACTCAC GCATCTTAGA TCTTTCATCC
    GGGGATTTAC TCTCAGATGT GGAAGGAAGC CAGAGTCTGA CGCACTCACG AACTGATGTT
    GAAATGCATG GCTGCCAGCT GAACACAAAA GCAGGAACCC CTGCTTTGAA ATGCCCTGGC
    ACGGGGATTG CCGTCTCTGG TCATCTCTGC CCTCGAAGCA GCTACAGCAG CAGTGAGCTG
    TCTCTTTCCA GCACCTGCAG CGAGTATTCC AGCAGCTCCT CCTACACGTG GCATGATGGA
    AAGAACCTCA GGAAAAGGCA GTCATCACAA AATTGGGACA AAAGGTTAAG TATTGACTCT
    TCGCTCCCGA GTGGCTTTGC TAGTCCTATG GATGAACTAC CTCCAACTCG CATCAAGGAA
    AGCCACATTT TGGAAGGACT AAGAAAGCTC CAGAAGCGAA AAGTGTTACT TGAACCCCCA
    TCAGTGATAA CCAAATGGGG TTATAAAGAT TGCATGAACT CCAATGAAGG AATCTATTCT
    CCAGGAATTA ATAGTAACAG CCTGAAGGAA TATCCACCCT GCAAACCCAC AGACACAAGG
    AGCCCCTGCA CGGATCCCCA CAAGGCATTT GTTTATGACA CGGATTCTCA TGACGAGGCT
    GATGAGGACG CTTCCTCCTT AGCCTCTGTC CAAGCGGTTC CAAACCAGGG CTGCAGGCTG
    CACAGTTGTA AATTAACCCA CAGTGTCTCT GACAGCCTCT TTGGCTGGGA GCTGAATGGC
    AAACACTGTT CAGAAGTCAC TTCCTCGGTC TACTCCAGGG AGAGGCCTGA GAAGCTGACC
    GGTTGTGCCA GCAACTGTCC CTTGGAGCAG AAGCTGTGCC CAGGCATGCA GGGGCCTCGG
    GTACAGAGAG AGAAGGTCTC ACCCAGCCAG GGCTGCCAGG CCCTCAGTCT CCAGCCCTCA
    GACACCGATG ACCCGGAGAC CCTGGATGAG CTGCACATCG AGAGCAGTGA TGAGAAAAGC
    CCTTCAGATG TGTCTGCAAC CGCGGACACT GACCAGTCCA CGGAGAGCCT GGATATCCTC
    ACAGGATTTG AAAAATCTTC ATGTGGGTCT CCCGAGGAGG AAGAAAACCA AGTACCCGTC
    CATTTAGAGA GCAGGCCAAA GACGTTTAGT TTCATTAAGC AACAGAGGGT TGTAAAACGG
    ACTTCCTCAG AAGAATGTAT TACTGTGATA TTTGATGCAG AAGACGGCGA GCCCATTGAG
    TTCAGCTCTC ACCAGACTGG CCTTGTTACT GTTACCAGAA AGGAGATTTC CATCCATCAG
    GCCCCTGCTG GGCCCCAGAT GGAACACACT GAACTTTCAC CTCAGGGAAT TGCTCATTTA
    CAGCCAGGGG CTGCAGCTAG AGACTACCCT TTTTTAAAGA GGTCTGAAGA GGAAACTGAA
    AGAAACATTC CACGAGATGA GGTAGATGAT CCTGCCATGA CACCTGCTGT GTCTTTCCAC
    CCCAGGACAG TGACGCAAAA TACTCAAAGA CTGGCCAAAC CGACACATGT CACACCATGC
    CAGAGTCATT CTCGGTCATC TGTGGGCACA GGCGTCTGTC AGAAGAAAAG TCTGACAAAA
    ATACCTACCA GGGGCAAGTC TTCACCTCAG AAATCGAGAG TAAGGGAGCC TGAAGCCTCC
    CTTATGATGC CCTCAGCTGG CTCAGTGACT CTTGAGAAAT CACCAGCTCC AGGGAAACTC
    TCACAGTTCA AAAAGACTGA AGGTCCAGCT CCCCTTTTTG ATTTGCAGCC AGATTCACAC
    ATTCCAAAAC CCCCCACCCA GCTTCCTCAT GGCTCCAAGA TGTCCAGCAG AAGGGATTGG
    GTCCAGAGCT CCAAGAATCA GATGCTAGCA TCACAGTTGC TGTCCAGGCC CCCCACTGAG
    CGGAGTGACG ATGGGGAGCC CCCCACGAGG GATAAACACT GTGATCCAGG GCCAGAGGCC
    GGGGTGAAAT CGCCCTCCCC ACCATCCCCT CCAGGCAGGT CGGTCTCCCT ACTCATCAGG
    CCCAGTTATG ACTTTTTGCC ACCACCTTCA TCTGCCAAAT CCGAAAGCAG GGTCTCCACC
    GAGACAGCAA GAATGGTATT GAAATCACCC CCTCTGAAAG GATCCTCTGC TCCTGTTATT
    TATTTTAACC AGACCCTGAC AGATGTGCAG GGGAAGAAAC CGTCTGTGGC CTTCAAAAAG
    CCCATCTTCA CTCCCTCACC ACCCTCCGCA GAAACAGCAA TCCAGATGAG ATGCCCTGCT
    CACAGCCCCT CCAGCTCATT TGCTGTGATG GTCCCGGAGC CCCCAAAAGT CTCTCCAAAG
    AGAAATGCCC CCAGAGCCCC ACCTCACCAA ACACTTGGGA CCACACAGAA CAACACAGGG
    TTACAGACTC CCAAGAACTA TGCTTCCCCC CGTGAGCCAC TGGAAATACC ATCCTCCAAA
    GGTGTATCTC CAGGGAGAAA AGAAAAGTTG AGTGGCAGTG TCTCTGCATC TTCCAAGCCT
    TCCTTCTTAG GGGTAAATGA GTCACCATCA TCTCAGGTTA ACAGTCCCTT ATCATCAGCA
    TCCTTCAAAA GCCATAACCC CCTACATGGT TGTCAAAACC TTCATGAGAG AGGACTGAAA
    ACTCGCTTCC CAGTTGGGCT CAAAGTTTTC ATGAAGTCTC CCCAGTTGCT CAGGAAAAGT
    TCCACGGTGC CAGGGAAACA GGAAAAAGAC AGTCTAAATG AAGCCTCGAA AACCTCTGTG
    GCTGTGAGCA AGGCTAAGCC AGGGGCCTCC AGAAATCCAG CCAGCCTTGA GACCACAGGA
    GGGGAAAGAA ATACGTCTCC AGTGAGTTTA CCAGCACAAG AGAGTTTGGC CAAAGGGCTT
    CCCCTGGAAA GAGCAATGCC AGAGTCACTG GAAAATAGCA TGCCTGGGGC TGATAGAAAA
    GATGGAGTAG AAAACAGGTC TGTAAAAAGA TCCCTGTCCT CCAGCAAACC ACACCTGAAA
    CCAGCTCTAG GCATGAACGG AGCCAAAGCC CGCAGCCAGA GTTTCAGTGC TCACTCAGGG
    GAAAAGCCCC CCACGCCCCC CACAGAAGGG CTGGGCAAAG TCCGGACTCA AATTATTACC
    AACACCGCTG AGAGAGGCAA TTCCCTCACC CGGCAGAGCT CTTCTGCCGA AGGCTCTCCC
    AGCAAGAGTG CGTCTACCCC TGTGTCTGAT GGTCTCCCCA GCACTGGGAA GCCCCTGGGA
    CACCCTTCCC CCAGGCAGGG TTCCTTGGGG AGCACAGGCA GCTCCAGCAG CCAGCATGGG
    AGCCCAAGCA AGTTGCCTTT GAGGATCCCT CCAAAATCCG AGGAGCCCCT CACCCCAGCT
    GGAACAGAAG AGCAGCAGGC CTATGCCCAG GGAGAAGGCC CCAGGGTGAC GGTACCTGAG
    GAACCAGGCA GCGACCACTG CAGGTGCCCA CCTACCCCAA CAGATAGCTC AGGAGGCCCA
    CAGAGCCTGG GGAGGACGCC ACATCCCAGC AGTTTCACAG CAAGCAGGAC CTCCAAGCTA
    GAAACTTCCG GAAGGTATCC AGATACTTCT ACAACCAGGG CCGGTGTCGT AAGCCCAGAA
    GCCCCGCTCT CACCCACAAT TGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA
    AAGGGCCAAG TGCAAACAAA GTCCACCTCT GTGGAAGCAA AACCGAAGCC GGGGCCTTCT
    TTTGCCAGCT GGTTCGGTTT TCGGAGGAGC AGACTCCCGG CTCTCAGTAG CAGGAAGATG
    GATGTCTCCA GAACCAAAGC AGAAAAGAAA GATGCAAAAG GTTTAGGGTT TGGAAACAAA
    CAGCTGAAAT CAGAGAGAAA GAAAGAGAAA AAGAAGCCTG AGCTACAGTG TGAGATGGAA
    AATGAACTTC ACAGGGATAT CGAGTTGGCA GATGGTCCAG ACAGTGGTTT GCAAAACAGA
    AATAACCCGA AAACACCGCC AGACATTTAT GATCAAGTGA AGTTTGAATC AAGAAATAGA
    CCCAGCCCTG TTCCATGTTC AGCGAAAGAT ACCTTTATGA CAGAACTTCT GAACAGAGTT
    GATAAGAAAG CAGCTCAACA GACAGAAAGT GGATCAAATA GTCTCTCCTG TAGGAGTGTG
    TTAAAGGGCA GCTCCCAGGG CTCCTGTCTC ACCAGCAGCT CTCTCAGCAC TCAAGGAAAC
    CACAAGAAAA ACATCAAAAC CAAAGCAGAT ATGGAAAAAC CGAAAGGCTC CCTGGTAAGA
    GAGGCAAATG ATCACCTGCA AGAGGATGAA GAAGATACAG TCGCAGATTC TGCATTTCAG
    AGCCATACCA TAGAAACAAA CTGCCAGATG AGGACCCTGG ACAGTGGGAT TGGAACCTTC
    CCACTCCCAG ACTCAGGAAC TCGTGCAGCG GGACGATACA TACGCCAAGC AGACTCCCCT
    GAGGACACTG ACCCCATCCT GTCTCTCCAA CCGGCCCTTT GTGCAGCCTC TTCCATCAGA
    GCCCAGACCC TTGAAAGAGA AGTGCCTTCC TCTGCAGATA GCCAGGGCTC TGCAGACAAT
    GCCATTGTTC ACTCCACATC CGACCCCATC ATGACTGCCA GAGGGACGCG ACCTCTTCAG
    AGTCTCCTCC CCAAACCAGC CTCCTCAGGA AAAATCAATT CCCAAATGAA GAGTGAAGCC
    GAGCCAAGGT CTCAAAATTG TTCACCATTC CAATATGTCG AAAACACAAT GGCGAGCAAG
    CCACTTCCAG CCTGGGAGGG TGATGACGCT GCTGCAGAGA CCCAGGACAA GGCACCCAGA
    ATGTGTGCAT ACTCTGCCAG CGGTAGCAGT GATAGTGACA GTGACCCGGA CTATGGAAAT
    AATGGTTTTG GAGCTGGAAG GGGCAAGTTA GTGAAAGCAA TGAAGAGCAC CACCCCAGAA
    ATTGAAACAA GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317808.1
    CDS367..6081
    Translation

    Target ORF information:

    RefSeq Version XM_015462322.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462322.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAGGGCA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TTTAGATAGC 
    AGTCTCGTGG AATACATGGA CTCCAACAAA TACATTGAAC ATCTGTTAAA TCAACTTGAG
    GAGCAACACA GAAGTCTCTG GAGGGAGAAG TTGGCTGTGG CCCGACTGCA GAGAGAAGTT
    GCCCAGAGAA GAAGTGAAGG GGCCATGCAT GAGAAGCTGA TACATGAATT GGAAGAGGAG
    AGACACTTGC GTCTTCAGAG TGAGAAGCGC TTGCAGGAGG TGACACTAGA GTCTGAGCGC
    AACAGAATTC AGATGCGCGG CCTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA
    AATCTACTAC AGAGTCAAGG ACCTCCGGAA CAGAAAAAAG AAGAAACTGT TAATATAATG
    GTCTATCAGG AAAGAGGAAG CAGGGAGGAA CTGAAACACT GTGCGGCTTT GGAAGGCCGT
    CACATGGCGC TAGATGAGGA CTCGAGGAGT GAAGGGAGCA GTGCAGATGA GGGGAAAGGC
    AAGACCAAAT GGCTGTTAGA AAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCATTGGCT
    TTGGAAAATG AAAATCAAAG GGAACAGTAT GAGAGATGTC TTGATGAGGT AGCCAACCAG
    GTTGTGCAAG CCCTGCTCAC TCAAAAGGAT CTCAGGGAGG AATGTGTAAA GTTGAAAACA
    AGAGTGTTTG ATTTGGAACA ACAGAATCGA ACGCTAAGCA TCCTTTTCCA ACAGCGAGTC
    AGACCAACTT CTGATCTCCT CCTCCAGAAA CTTCACTCAC GCATCTTAGA TCTTTCATCC
    GGGGATTTAC TCTCAGATGT GGAAGGAAGC CAGAGTCTGA CGCACTCACG AACTGATGTT
    GAAATGCATG GCTGCCAGCT GAACACAAAA GCAGGAACCC CTGCTTTGAA ATGCCCTGGC
    ACGGGGATTG CCGTCTCTGG TCATCTCTGC CCTCGAAGCA GCTACAGCAG CAGTGAGCTG
    TCTCTTTCCA GCACCTGCAG CGAGTATTCC AGCAGCTCCT CCTACACGTG GCATGATGGA
    AAGAACCTCA GGAAAAGGCA GTCATCACAA AATTGGGACA AAAGGTTAAG TATTGACTCT
    TCGCTCCCGA GTGGCTTTGC TAGTCCTATG GATGAACTAC CTCCAACTCG CATCAAGGAA
    AGCCACATTT TGGAAGGACT AAGAAAGCTC CAGAAGCGAA AAGTGTTACT TGAACCCCCA
    TCAGTGATAA CCAAATGGGG TTATAAAGAT TGCATGAACT CCAATGAAGG AATCTATTCT
    CCAGGAATTA ATAGTAACAG CCTGAAGGAA TATCCACCCT GCAAACCCAC AGACACAAGG
    AGCCCCTGCA CGGATCCCCA CAAGGCATTT GTTTATGACA CGGATTCTCA TGACGAGGCT
    GATGAGGACG CTTCCTCCTT AGCCTCTGTC CAAGCGGTTC CAAACCAGGG CTGCAGGCTG
    CACAGTTGTA AATTAACCCA CAGTGTCTCT GACAGCCTCT TTGGCTGGGA GCTGAATGGC
    AAACACTGTT CAGAAGTCAC TTCCTCGGTC TACTCCAGGG AGAGGCCTGA GAAGCTGACC
    GGTTGTGCCA GCAACTGTCC CTTGGAGCAG AAGCTGTGCC CAGGCATGCA GGGGCCTCGG
    GTACAGAGAG AGAAGGTCTC ACCCAGCCAG GGCTGCCAGG CCCTCAGTCT CCAGCCCTCA
    GACACCGATG ACCCGGAGAC CCTGGATGAG CTGCACATCG AGAGCAGTGA TGAGAAAAGC
    CCTTCAGATG TGTCTGCAAC CGCGGACACT GACCAGTCCA CGGAGAGCCT GGATATCCTC
    ACAGGATTTG AAAAATCTTC ATGTGGGTCT CCCGAGGAGG AAGAAAACCA AGTACCCGTC
    CATTTAGAGA GCAGGCCAAA GACGTTTAGT TTCATTAAGC AACAGAGGGT TGTAAAACGG
    ACTTCCTCAG AAGAATGTAT TACTGTGATA TTTGATGCAG AAGACGGCGA GCCCATTGAG
    TTCAGCTCTC ACCAGACTGG CCTTGTTACT GTTACCAGAA AGGAGATTTC CATCCATCAG
    GCCCCTGCTG GGCCCCAGAT GGAACACACT GAACTTTCAC CTCAGGGAAT TGCTCATTTA
    CAGCCAGGGG CTGCAGCTAG AGACTACCCT TTTTTAAAGA GGTCTGAAGA GGAAACTGAA
    AGAAACATTC CACGAGATGA GGTAGATGAT CCTGCCATGA CACCTGCTGT GTCTTTCCAC
    CCCAGGACAG TGACGCAAAA TACTCAAAGA CTGGCCAAAC CGACACATGT CACACCATGC
    CAGAGTCATT CTCGGTCATC TGTGGGCACA GGCGTCTGTC AGAAGAAAAG TCTGACAAAA
    ATACCTACCA GGGGCAAGTC TTCACCTCAG AAATCGAGAG TAAGGGAGCC TGAAGCCTCC
    CTTATGATGC CCTCAGCTGG CTCAGTGACT CTTGAGAAAT CACCAGCTCC AGGGAAACTC
    TCACAGTTCA AAAAGACTGA AGGTCCAGCT CCCCTTTTTG ATTTGCAGCC AGATTCACAC
    ATTCCAAAAC CCCCCACCCA GCTTCCTCAT GGCTCCAAGA TGTCCAGCAG AAGGGATTGG
    GTCCAGAGCT CCAAGAATCA GATGCTAGCA TCACAGTTGC TGTCCAGGCC CCCCACTGAG
    CGGAGTGACG ATGGGGAGCC CCCCACGAGG GATAAACACT GTGATCCAGG GCCAGAGGCC
    GGGGTGAAAT CGCCCTCCCC ACCATCCCCT CCAGGCAGGT CGGTCTCCCT ACTCATCAGG
    CCCAGTTATG ACTTTTTGCC ACCACCTTCA TCTGCCAAAT CCGAAAGCAG GGTCTCCACC
    GAGACAGCAA GAATGGTATT GAAATCACCC CCTCTGAAAG GATCCTCTGC TCCTGTTATT
    TATTTTAACC AGACCCTGAC AGATGTGCAG GGGAAGAAAC CGTCTGTGGC CTTCAAAAAG
    CCCATCTTCA CTCCCTCACC ACCCTCCGCA GAAACAGCAA TCCAGATGAG ATGCCCTGCT
    CACAGCCCCT CCAGCTCATT TGCTGTGATG GTCCCGGAGC CCCCAAAAGT CTCTCCAAAG
    AGAAATGCCC CCAGAGCCCC ACCTCACCAA ACACTTGGGA CCACACAGAA CAACACAGGG
    TTACAGACTC CCAAGAACTA TGCTTCCCCC CGTGAGCCAC TGGAAATACC ATCCTCCAAA
    GGTGTATCTC CAGGGAGAAA AGAAAAGTTG AGTGGCAGTG TCTCTGCATC TTCCAAGCCT
    TCCTTCTTAG GGGTAAATGA GTCACCATCA TCTCAGGTTA ACAGTCCCTT ATCATCAGCA
    TCCTTCAAAA GCCATAACCC CCTACATGGT TGTCAAAACC TTCATGAGAG AGGACTGAAA
    ACTCGCTTCC CAGTTGGGCT CAAAGTTTTC ATGAAGTCTC CCCAGTTGCT CAGGAAAAGT
    TCCACGGTGC CAGGGAAACA GGAAAAAGAC AGTCTAAATG AAGCCTCGAA AACCTCTGTG
    GCTGTGAGCA AGGCTAAGCC AGGGGCCTCC AGAAATCCAG CCAGCCTTGA GACCACAGGA
    GGGGAAAGAA ATACGTCTCC AGTGAGTTTA CCAGCACAAG AGAGTTTGGC CAAAGGGCTT
    CCCCTGGAAA GAGCAATGCC AGAGTCACTG GAAAATAGCA TGCCTGGGGC TGATAGAAAA
    GATGGAGTAG AAAACAGGTC TGTAAAAAGA TCCCTGTCCT CCAGCAAACC ACACCTGAAA
    CCAGCTCTAG GCATGAACGG AGCCAAAGCC CGCAGCCAGA GTTTCAGTGC TCACTCAGGG
    GAAAAGCCCC CCACGCCCCC CACAGAAGGG CTGGGCAAAG TCCGGACTCA AATTATTACC
    AACACCGCTG AGAGAGGCAA TTCCCTCACC CGGCAGAGCT CTTCTGCCGA AGGCTCTCCC
    AGCAAGAGTG CGTCTACCCC TGTGTCTGAT GGTCTCCCCA GCACTGGGAA GCCCCTGGGA
    CACCCTTCCC CCAGGCAGGG TTCCTTGGGG AGCACAGGCA GCTCCAGCAG CCAGCATGGG
    AGCCCAAGCA AGTTGCCTTT GAGGATCCCT CCAAAATCCG AGGAGCCCCT CACCCCAGCT
    GGAACAGAAG AGCAGCAGGC CTATGCCCAG GGAGAAGGCC CCAGGGTGAC GGTACCTGAG
    GAACCAGGCA GCGACCACTG CAGGTGCCCA CCTACCCCAA CAGATAGCTC AGGAGGCCCA
    CAGAGCCTGG GGAGGACGCC ACATCCCAGC AGTTTCACAG CAAGCAGGAC CTCCAAGCTA
    GAAACTTCCG GAAGGTATCC AGATACTTCT ACAACCAGGG CCGGTGTCGT AAGCCCAGAA
    GCCCCGCTCT CACCCACAAT TGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA
    AAGGGCCAAG TGCAAACAAA GTCCACCTCT GTGGAAGCAA AACCGAAGCC GGGGCCTTCT
    TTTGCCAGCT GGTTCGGTTT TCGGAGGAGC AGACTCCCGG CTCTCAGTAG CAGGAAGATG
    GATGTCTCCA GAACCAAAGC AGAAAAGAAA GATGCAAAAG GTTTAGGGTT TGGAAACAAA
    CAGCTGAAAT CAGAGAGAAA GAAAGAGAAA AAGAAGCCTG AGCTACAGTG TGAGATGGAA
    AATGAACTTC ACAGGGATAT CGAGTTGGCA GATGGTCCAG ACAGTGGTTT GCAAAACAGA
    AATAACCCGA AAACACCGCC AGACATTTAT GATCAAGTGA AGTTTGAATC AAGAAATAGA
    CCCAGCCCTG TTCCATGTTC AGCGAAAGAT ACCTTTATGA CAGAACTTCT GAACAGAGTT
    GATAAGAAAG CAGCTCAACA GACAGAAAGT GGATCAAATA GTCTCTCCTG TAGGAGTGTG
    TTAAAGGGCA GCTCCCAGGG CTCCTGTCTC ACCAGCAGCT CTCTCAGCAC TCAAGGAAAC
    CACAAGAAAA ACATCAAAAC CAAAGCAGAT ATGGAAAAAC CGAAAGGCTC CCTGGTAAGA
    GAGGCAAATG ATCACCTGCA AGAGGATGAA GAAGATACAG TCGCAGATTC TGCATTTCAG
    AGCCATACCA TAGAAACAAA CTGCCAGATG AGGACCCTGG ACAGTGGGAT TGGAACCTTC
    CCACTCCCAG ACTCAGGAAC TCGTGCAGCG GGACGATACA TACGCCAAGC AGACTCCCCT
    GAGGACACTG ACCCCATCCT GTCTCTCCAA CCGGCCCTTT GTGCAGCCTC TTCCATCAGA
    GCCCAGACCC TTGAAAGAGA AGTGCCTTCC TCTGCAGATA GCCAGGGCTC TGCAGACAAT
    GCCATTGTTC ACTCCACATC CGACCCCATC ATGACTGCCA GAGGGACGCG ACCTCTTCAG
    AGTCTCCTCC CCAAACCAGC CTCCTCAGGA AAAATCAATT CCCAAATGAA GAGTGAAGCC
    GAGCCAAGGT CTCAAAATTG TTCACCATTC CAATATGTCG AAAACACAAT GGCGAGCAAG
    CCACTTCCAG CCTGGGAGGG TGATGACGCT GCTGCAGAGA CCCAGGACAA GGCACCCAGA
    ATGTGTGCAT ACTCTGCCAG CGGTAGCAGT GATAGTGACA GTGACCCGGA CTATGGAAAT
    AATGGTTTTG GAGCTGGAAG GGGCAAGTTA GTGAAAGCAA TGAAGAGCAC CACCCCAGAA
    ATTGAAACAA GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16499
    Clone ID Related Accession (Same CDS sequence) XM_015462322.1 , XM_002685418.4
    Accession Version XM_002685418.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5715bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nck-associated protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103841.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002685418.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAGGGCA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TTTAGATAGC 
    AGTCTCGTGG AATACATGGA CTCCAACAAA TACATTGAAC ATCTGTTAAA TCAACTTGAG
    GAGCAACACA GAAGTCTCTG GAGGGAGAAG TTGGCTGTGG CCCGACTGCA GAGAGAAGTT
    GCCCAGAGAA GAAGTGAAGG GGCCATGCAT GAGAAGCTGA TACATGAATT GGAAGAGGAG
    AGACACTTGC GTCTTCAGAG TGAGAAGCGC TTGCAGGAGG TGACACTAGA GTCTGAGCGC
    AACAGAATTC AGATGCGCGG CCTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA
    AATCTACTAC AGAGTCAAGG ACCTCCGGAA CAGAAAAAAG AAGAAACTGT TAATATAATG
    GTCTATCAGG AAAGAGGAAG CAGGGAGGAA CTGAAACACT GTGCGGCTTT GGAAGGCCGT
    CACATGGCGC TAGATGAGGA CTCGAGGAGT GAAGGGAGCA GTGCAGATGA GGGGAAAGGC
    AAGACCAAAT GGCTGTTAGA AAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCATTGGCT
    TTGGAAAATG AAAATCAAAG GGAACAGTAT GAGAGATGTC TTGATGAGGT AGCCAACCAG
    GTTGTGCAAG CCCTGCTCAC TCAAAAGGAT CTCAGGGAGG AATGTGTAAA GTTGAAAACA
    AGAGTGTTTG ATTTGGAACA ACAGAATCGA ACGCTAAGCA TCCTTTTCCA ACAGCGAGTC
    AGACCAACTT CTGATCTCCT CCTCCAGAAA CTTCACTCAC GCATCTTAGA TCTTTCATCC
    GGGGATTTAC TCTCAGATGT GGAAGGAAGC CAGAGTCTGA CGCACTCACG AACTGATGTT
    GAAATGCATG GCTGCCAGCT GAACACAAAA GCAGGAACCC CTGCTTTGAA ATGCCCTGGC
    ACGGGGATTG CCGTCTCTGG TCATCTCTGC CCTCGAAGCA GCTACAGCAG CAGTGAGCTG
    TCTCTTTCCA GCACCTGCAG CGAGTATTCC AGCAGCTCCT CCTACACGTG GCATGATGGA
    AAGAACCTCA GGAAAAGGCA GTCATCACAA AATTGGGACA AAAGGTTAAG TATTGACTCT
    TCGCTCCCGA GTGGCTTTGC TAGTCCTATG GATGAACTAC CTCCAACTCG CATCAAGGAA
    AGCCACATTT TGGAAGGACT AAGAAAGCTC CAGAAGCGAA AAGTGTTACT TGAACCCCCA
    TCAGTGATAA CCAAATGGGG TTATAAAGAT TGCATGAACT CCAATGAAGG AATCTATTCT
    CCAGGAATTA ATAGTAACAG CCTGAAGGAA TATCCACCCT GCAAACCCAC AGACACAAGG
    AGCCCCTGCA CGGATCCCCA CAAGGCATTT GTTTATGACA CGGATTCTCA TGACGAGGCT
    GATGAGGACG CTTCCTCCTT AGCCTCTGTC CAAGCGGTTC CAAACCAGGG CTGCAGGCTG
    CACAGTTGTA AATTAACCCA CAGTGTCTCT GACAGCCTCT TTGGCTGGGA GCTGAATGGC
    AAACACTGTT CAGAAGTCAC TTCCTCGGTC TACTCCAGGG AGAGGCCTGA GAAGCTGACC
    GGTTGTGCCA GCAACTGTCC CTTGGAGCAG AAGCTGTGCC CAGGCATGCA GGGGCCTCGG
    GTACAGAGAG AGAAGGTCTC ACCCAGCCAG GGCTGCCAGG CCCTCAGTCT CCAGCCCTCA
    GACACCGATG ACCCGGAGAC CCTGGATGAG CTGCACATCG AGAGCAGTGA TGAGAAAAGC
    CCTTCAGATG TGTCTGCAAC CGCGGACACT GACCAGTCCA CGGAGAGCCT GGATATCCTC
    ACAGGATTTG AAAAATCTTC ATGTGGGTCT CCCGAGGAGG AAGAAAACCA AGTACCCGTC
    CATTTAGAGA GCAGGCCAAA GACGTTTAGT TTCATTAAGC AACAGAGGGT TGTAAAACGG
    ACTTCCTCAG AAGAATGTAT TACTGTGATA TTTGATGCAG AAGACGGCGA GCCCATTGAG
    TTCAGCTCTC ACCAGACTGG CCTTGTTACT GTTACCAGAA AGGAGATTTC CATCCATCAG
    GCCCCTGCTG GGCCCCAGAT GGAACACACT GAACTTTCAC CTCAGGGAAT TGCTCATTTA
    CAGCCAGGGG CTGCAGCTAG AGACTACCCT TTTTTAAAGA GGTCTGAAGA GGAAACTGAA
    AGAAACATTC CACGAGATGA GGTAGATGAT CCTGCCATGA CACCTGCTGT GTCTTTCCAC
    CCCAGGACAG TGACGCAAAA TACTCAAAGA CTGGCCAAAC CGACACATGT CACACCATGC
    CAGAGTCATT CTCGGTCATC TGTGGGCACA GGCGTCTGTC AGAAGAAAAG TCTGACAAAA
    ATACCTACCA GGGGCAAGTC TTCACCTCAG AAATCGAGAG TAAGGGAGCC TGAAGCCTCC
    CTTATGATGC CCTCAGCTGG CTCAGTGACT CTTGAGAAAT CACCAGCTCC AGGGAAACTC
    TCACAGTTCA AAAAGACTGA AGGTCCAGCT CCCCTTTTTG ATTTGCAGCC AGATTCACAC
    ATTCCAAAAC CCCCCACCCA GCTTCCTCAT GGCTCCAAGA TGTCCAGCAG AAGGGATTGG
    GTCCAGAGCT CCAAGAATCA GATGCTAGCA TCACAGTTGC TGTCCAGGCC CCCCACTGAG
    CGGAGTGACG ATGGGGAGCC CCCCACGAGG GATAAACACT GTGATCCAGG GCCAGAGGCC
    GGGGTGAAAT CGCCCTCCCC ACCATCCCCT CCAGGCAGGT CGGTCTCCCT ACTCATCAGG
    CCCAGTTATG ACTTTTTGCC ACCACCTTCA TCTGCCAAAT CCGAAAGCAG GGTCTCCACC
    GAGACAGCAA GAATGGTATT GAAATCACCC CCTCTGAAAG GATCCTCTGC TCCTGTTATT
    TATTTTAACC AGACCCTGAC AGATGTGCAG GGGAAGAAAC CGTCTGTGGC CTTCAAAAAG
    CCCATCTTCA CTCCCTCACC ACCCTCCGCA GAAACAGCAA TCCAGATGAG ATGCCCTGCT
    CACAGCCCCT CCAGCTCATT TGCTGTGATG GTCCCGGAGC CCCCAAAAGT CTCTCCAAAG
    AGAAATGCCC CCAGAGCCCC ACCTCACCAA ACACTTGGGA CCACACAGAA CAACACAGGG
    TTACAGACTC CCAAGAACTA TGCTTCCCCC CGTGAGCCAC TGGAAATACC ATCCTCCAAA
    GGTGTATCTC CAGGGAGAAA AGAAAAGTTG AGTGGCAGTG TCTCTGCATC TTCCAAGCCT
    TCCTTCTTAG GGGTAAATGA GTCACCATCA TCTCAGGTTA ACAGTCCCTT ATCATCAGCA
    TCCTTCAAAA GCCATAACCC CCTACATGGT TGTCAAAACC TTCATGAGAG AGGACTGAAA
    ACTCGCTTCC CAGTTGGGCT CAAAGTTTTC ATGAAGTCTC CCCAGTTGCT CAGGAAAAGT
    TCCACGGTGC CAGGGAAACA GGAAAAAGAC AGTCTAAATG AAGCCTCGAA AACCTCTGTG
    GCTGTGAGCA AGGCTAAGCC AGGGGCCTCC AGAAATCCAG CCAGCCTTGA GACCACAGGA
    GGGGAAAGAA ATACGTCTCC AGTGAGTTTA CCAGCACAAG AGAGTTTGGC CAAAGGGCTT
    CCCCTGGAAA GAGCAATGCC AGAGTCACTG GAAAATAGCA TGCCTGGGGC TGATAGAAAA
    GATGGAGTAG AAAACAGGTC TGTAAAAAGA TCCCTGTCCT CCAGCAAACC ACACCTGAAA
    CCAGCTCTAG GCATGAACGG AGCCAAAGCC CGCAGCCAGA GTTTCAGTGC TCACTCAGGG
    GAAAAGCCCC CCACGCCCCC CACAGAAGGG CTGGGCAAAG TCCGGACTCA AATTATTACC
    AACACCGCTG AGAGAGGCAA TTCCCTCACC CGGCAGAGCT CTTCTGCCGA AGGCTCTCCC
    AGCAAGAGTG CGTCTACCCC TGTGTCTGAT GGTCTCCCCA GCACTGGGAA GCCCCTGGGA
    CACCCTTCCC CCAGGCAGGG TTCCTTGGGG AGCACAGGCA GCTCCAGCAG CCAGCATGGG
    AGCCCAAGCA AGTTGCCTTT GAGGATCCCT CCAAAATCCG AGGAGCCCCT CACCCCAGCT
    GGAACAGAAG AGCAGCAGGC CTATGCCCAG GGAGAAGGCC CCAGGGTGAC GGTACCTGAG
    GAACCAGGCA GCGACCACTG CAGGTGCCCA CCTACCCCAA CAGATAGCTC AGGAGGCCCA
    CAGAGCCTGG GGAGGACGCC ACATCCCAGC AGTTTCACAG CAAGCAGGAC CTCCAAGCTA
    GAAACTTCCG GAAGGTATCC AGATACTTCT ACAACCAGGG CCGGTGTCGT AAGCCCAGAA
    GCCCCGCTCT CACCCACAAT TGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA
    AAGGGCCAAG TGCAAACAAA GTCCACCTCT GTGGAAGCAA AACCGAAGCC GGGGCCTTCT
    TTTGCCAGCT GGTTCGGTTT TCGGAGGAGC AGACTCCCGG CTCTCAGTAG CAGGAAGATG
    GATGTCTCCA GAACCAAAGC AGAAAAGAAA GATGCAAAAG GTTTAGGGTT TGGAAACAAA
    CAGCTGAAAT CAGAGAGAAA GAAAGAGAAA AAGAAGCCTG AGCTACAGTG TGAGATGGAA
    AATGAACTTC ACAGGGATAT CGAGTTGGCA GATGGTCCAG ACAGTGGTTT GCAAAACAGA
    AATAACCCGA AAACACCGCC AGACATTTAT GATCAAGTGA AGTTTGAATC AAGAAATAGA
    CCCAGCCCTG TTCCATGTTC AGCGAAAGAT ACCTTTATGA CAGAACTTCT GAACAGAGTT
    GATAAGAAAG CAGCTCAACA GACAGAAAGT GGATCAAATA GTCTCTCCTG TAGGAGTGTG
    TTAAAGGGCA GCTCCCAGGG CTCCTGTCTC ACCAGCAGCT CTCTCAGCAC TCAAGGAAAC
    CACAAGAAAA ACATCAAAAC CAAAGCAGAT ATGGAAAAAC CGAAAGGCTC CCTGGTAAGA
    GAGGCAAATG ATCACCTGCA AGAGGATGAA GAAGATACAG TCGCAGATTC TGCATTTCAG
    AGCCATACCA TAGAAACAAA CTGCCAGATG AGGACCCTGG ACAGTGGGAT TGGAACCTTC
    CCACTCCCAG ACTCAGGAAC TCGTGCAGCG GGACGATACA TACGCCAAGC AGACTCCCCT
    GAGGACACTG ACCCCATCCT GTCTCTCCAA CCGGCCCTTT GTGCAGCCTC TTCCATCAGA
    GCCCAGACCC TTGAAAGAGA AGTGCCTTCC TCTGCAGATA GCCAGGGCTC TGCAGACAAT
    GCCATTGTTC ACTCCACATC CGACCCCATC ATGACTGCCA GAGGGACGCG ACCTCTTCAG
    AGTCTCCTCC CCAAACCAGC CTCCTCAGGA AAAATCAATT CCCAAATGAA GAGTGAAGCC
    GAGCCAAGGT CTCAAAATTG TTCACCATTC CAATATGTCG AAAACACAAT GGCGAGCAAG
    CCACTTCCAG CCTGGGAGGG TGATGACGCT GCTGCAGAGA CCCAGGACAA GGCACCCAGA
    ATGTGTGCAT ACTCTGCCAG CGGTAGCAGT GATAGTGACA GTGACCCGGA CTATGGAAAT
    AATGGTTTTG GAGCTGGAAG GGGCAAGTTA GTGAAAGCAA TGAAGAGCAC CACCCCAGAA
    ATTGAAACAA GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002685464.2
    CDS367..6081
    Translation

    Target ORF information:

    RefSeq Version XM_002685418.4
    Organism Bos taurus(cattle)
    Definition Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002685418.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAGGGCA AGAGACAGCT TGAGAAAAGG GACTTTGGAA AAAGGCTGTC TTTAGATAGC 
    AGTCTCGTGG AATACATGGA CTCCAACAAA TACATTGAAC ATCTGTTAAA TCAACTTGAG
    GAGCAACACA GAAGTCTCTG GAGGGAGAAG TTGGCTGTGG CCCGACTGCA GAGAGAAGTT
    GCCCAGAGAA GAAGTGAAGG GGCCATGCAT GAGAAGCTGA TACATGAATT GGAAGAGGAG
    AGACACTTGC GTCTTCAGAG TGAGAAGCGC TTGCAGGAGG TGACACTAGA GTCTGAGCGC
    AACAGAATTC AGATGCGCGG CCTGCAGCAG CAGTTCTCCA GGATGGAAGA AACAGTACGA
    AATCTACTAC AGAGTCAAGG ACCTCCGGAA CAGAAAAAAG AAGAAACTGT TAATATAATG
    GTCTATCAGG AAAGAGGAAG CAGGGAGGAA CTGAAACACT GTGCGGCTTT GGAAGGCCGT
    CACATGGCGC TAGATGAGGA CTCGAGGAGT GAAGGGAGCA GTGCAGATGA GGGGAAAGGC
    AAGACCAAAT GGCTGTTAGA AAGATTGAAA GCTCTAGAGG CAGAGAATTC AGCATTGGCT
    TTGGAAAATG AAAATCAAAG GGAACAGTAT GAGAGATGTC TTGATGAGGT AGCCAACCAG
    GTTGTGCAAG CCCTGCTCAC TCAAAAGGAT CTCAGGGAGG AATGTGTAAA GTTGAAAACA
    AGAGTGTTTG ATTTGGAACA ACAGAATCGA ACGCTAAGCA TCCTTTTCCA ACAGCGAGTC
    AGACCAACTT CTGATCTCCT CCTCCAGAAA CTTCACTCAC GCATCTTAGA TCTTTCATCC
    GGGGATTTAC TCTCAGATGT GGAAGGAAGC CAGAGTCTGA CGCACTCACG AACTGATGTT
    GAAATGCATG GCTGCCAGCT GAACACAAAA GCAGGAACCC CTGCTTTGAA ATGCCCTGGC
    ACGGGGATTG CCGTCTCTGG TCATCTCTGC CCTCGAAGCA GCTACAGCAG CAGTGAGCTG
    TCTCTTTCCA GCACCTGCAG CGAGTATTCC AGCAGCTCCT CCTACACGTG GCATGATGGA
    AAGAACCTCA GGAAAAGGCA GTCATCACAA AATTGGGACA AAAGGTTAAG TATTGACTCT
    TCGCTCCCGA GTGGCTTTGC TAGTCCTATG GATGAACTAC CTCCAACTCG CATCAAGGAA
    AGCCACATTT TGGAAGGACT AAGAAAGCTC CAGAAGCGAA AAGTGTTACT TGAACCCCCA
    TCAGTGATAA CCAAATGGGG TTATAAAGAT TGCATGAACT CCAATGAAGG AATCTATTCT
    CCAGGAATTA ATAGTAACAG CCTGAAGGAA TATCCACCCT GCAAACCCAC AGACACAAGG
    AGCCCCTGCA CGGATCCCCA CAAGGCATTT GTTTATGACA CGGATTCTCA TGACGAGGCT
    GATGAGGACG CTTCCTCCTT AGCCTCTGTC CAAGCGGTTC CAAACCAGGG CTGCAGGCTG
    CACAGTTGTA AATTAACCCA CAGTGTCTCT GACAGCCTCT TTGGCTGGGA GCTGAATGGC
    AAACACTGTT CAGAAGTCAC TTCCTCGGTC TACTCCAGGG AGAGGCCTGA GAAGCTGACC
    GGTTGTGCCA GCAACTGTCC CTTGGAGCAG AAGCTGTGCC CAGGCATGCA GGGGCCTCGG
    GTACAGAGAG AGAAGGTCTC ACCCAGCCAG GGCTGCCAGG CCCTCAGTCT CCAGCCCTCA
    GACACCGATG ACCCGGAGAC CCTGGATGAG CTGCACATCG AGAGCAGTGA TGAGAAAAGC
    CCTTCAGATG TGTCTGCAAC CGCGGACACT GACCAGTCCA CGGAGAGCCT GGATATCCTC
    ACAGGATTTG AAAAATCTTC ATGTGGGTCT CCCGAGGAGG AAGAAAACCA AGTACCCGTC
    CATTTAGAGA GCAGGCCAAA GACGTTTAGT TTCATTAAGC AACAGAGGGT TGTAAAACGG
    ACTTCCTCAG AAGAATGTAT TACTGTGATA TTTGATGCAG AAGACGGCGA GCCCATTGAG
    TTCAGCTCTC ACCAGACTGG CCTTGTTACT GTTACCAGAA AGGAGATTTC CATCCATCAG
    GCCCCTGCTG GGCCCCAGAT GGAACACACT GAACTTTCAC CTCAGGGAAT TGCTCATTTA
    CAGCCAGGGG CTGCAGCTAG AGACTACCCT TTTTTAAAGA GGTCTGAAGA GGAAACTGAA
    AGAAACATTC CACGAGATGA GGTAGATGAT CCTGCCATGA CACCTGCTGT GTCTTTCCAC
    CCCAGGACAG TGACGCAAAA TACTCAAAGA CTGGCCAAAC CGACACATGT CACACCATGC
    CAGAGTCATT CTCGGTCATC TGTGGGCACA GGCGTCTGTC AGAAGAAAAG TCTGACAAAA
    ATACCTACCA GGGGCAAGTC TTCACCTCAG AAATCGAGAG TAAGGGAGCC TGAAGCCTCC
    CTTATGATGC CCTCAGCTGG CTCAGTGACT CTTGAGAAAT CACCAGCTCC AGGGAAACTC
    TCACAGTTCA AAAAGACTGA AGGTCCAGCT CCCCTTTTTG ATTTGCAGCC AGATTCACAC
    ATTCCAAAAC CCCCCACCCA GCTTCCTCAT GGCTCCAAGA TGTCCAGCAG AAGGGATTGG
    GTCCAGAGCT CCAAGAATCA GATGCTAGCA TCACAGTTGC TGTCCAGGCC CCCCACTGAG
    CGGAGTGACG ATGGGGAGCC CCCCACGAGG GATAAACACT GTGATCCAGG GCCAGAGGCC
    GGGGTGAAAT CGCCCTCCCC ACCATCCCCT CCAGGCAGGT CGGTCTCCCT ACTCATCAGG
    CCCAGTTATG ACTTTTTGCC ACCACCTTCA TCTGCCAAAT CCGAAAGCAG GGTCTCCACC
    GAGACAGCAA GAATGGTATT GAAATCACCC CCTCTGAAAG GATCCTCTGC TCCTGTTATT
    TATTTTAACC AGACCCTGAC AGATGTGCAG GGGAAGAAAC CGTCTGTGGC CTTCAAAAAG
    CCCATCTTCA CTCCCTCACC ACCCTCCGCA GAAACAGCAA TCCAGATGAG ATGCCCTGCT
    CACAGCCCCT CCAGCTCATT TGCTGTGATG GTCCCGGAGC CCCCAAAAGT CTCTCCAAAG
    AGAAATGCCC CCAGAGCCCC ACCTCACCAA ACACTTGGGA CCACACAGAA CAACACAGGG
    TTACAGACTC CCAAGAACTA TGCTTCCCCC CGTGAGCCAC TGGAAATACC ATCCTCCAAA
    GGTGTATCTC CAGGGAGAAA AGAAAAGTTG AGTGGCAGTG TCTCTGCATC TTCCAAGCCT
    TCCTTCTTAG GGGTAAATGA GTCACCATCA TCTCAGGTTA ACAGTCCCTT ATCATCAGCA
    TCCTTCAAAA GCCATAACCC CCTACATGGT TGTCAAAACC TTCATGAGAG AGGACTGAAA
    ACTCGCTTCC CAGTTGGGCT CAAAGTTTTC ATGAAGTCTC CCCAGTTGCT CAGGAAAAGT
    TCCACGGTGC CAGGGAAACA GGAAAAAGAC AGTCTAAATG AAGCCTCGAA AACCTCTGTG
    GCTGTGAGCA AGGCTAAGCC AGGGGCCTCC AGAAATCCAG CCAGCCTTGA GACCACAGGA
    GGGGAAAGAA ATACGTCTCC AGTGAGTTTA CCAGCACAAG AGAGTTTGGC CAAAGGGCTT
    CCCCTGGAAA GAGCAATGCC AGAGTCACTG GAAAATAGCA TGCCTGGGGC TGATAGAAAA
    GATGGAGTAG AAAACAGGTC TGTAAAAAGA TCCCTGTCCT CCAGCAAACC ACACCTGAAA
    CCAGCTCTAG GCATGAACGG AGCCAAAGCC CGCAGCCAGA GTTTCAGTGC TCACTCAGGG
    GAAAAGCCCC CCACGCCCCC CACAGAAGGG CTGGGCAAAG TCCGGACTCA AATTATTACC
    AACACCGCTG AGAGAGGCAA TTCCCTCACC CGGCAGAGCT CTTCTGCCGA AGGCTCTCCC
    AGCAAGAGTG CGTCTACCCC TGTGTCTGAT GGTCTCCCCA GCACTGGGAA GCCCCTGGGA
    CACCCTTCCC CCAGGCAGGG TTCCTTGGGG AGCACAGGCA GCTCCAGCAG CCAGCATGGG
    AGCCCAAGCA AGTTGCCTTT GAGGATCCCT CCAAAATCCG AGGAGCCCCT CACCCCAGCT
    GGAACAGAAG AGCAGCAGGC CTATGCCCAG GGAGAAGGCC CCAGGGTGAC GGTACCTGAG
    GAACCAGGCA GCGACCACTG CAGGTGCCCA CCTACCCCAA CAGATAGCTC AGGAGGCCCA
    CAGAGCCTGG GGAGGACGCC ACATCCCAGC AGTTTCACAG CAAGCAGGAC CTCCAAGCTA
    GAAACTTCCG GAAGGTATCC AGATACTTCT ACAACCAGGG CCGGTGTCGT AAGCCCAGAA
    GCCCCGCTCT CACCCACAAT TGAAGAAAAG GTCATGTTGT GCATTCAGGA AAATGTGGAA
    AAGGGCCAAG TGCAAACAAA GTCCACCTCT GTGGAAGCAA AACCGAAGCC GGGGCCTTCT
    TTTGCCAGCT GGTTCGGTTT TCGGAGGAGC AGACTCCCGG CTCTCAGTAG CAGGAAGATG
    GATGTCTCCA GAACCAAAGC AGAAAAGAAA GATGCAAAAG GTTTAGGGTT TGGAAACAAA
    CAGCTGAAAT CAGAGAGAAA GAAAGAGAAA AAGAAGCCTG AGCTACAGTG TGAGATGGAA
    AATGAACTTC ACAGGGATAT CGAGTTGGCA GATGGTCCAG ACAGTGGTTT GCAAAACAGA
    AATAACCCGA AAACACCGCC AGACATTTAT GATCAAGTGA AGTTTGAATC AAGAAATAGA
    CCCAGCCCTG TTCCATGTTC AGCGAAAGAT ACCTTTATGA CAGAACTTCT GAACAGAGTT
    GATAAGAAAG CAGCTCAACA GACAGAAAGT GGATCAAATA GTCTCTCCTG TAGGAGTGTG
    TTAAAGGGCA GCTCCCAGGG CTCCTGTCTC ACCAGCAGCT CTCTCAGCAC TCAAGGAAAC
    CACAAGAAAA ACATCAAAAC CAAAGCAGAT ATGGAAAAAC CGAAAGGCTC CCTGGTAAGA
    GAGGCAAATG ATCACCTGCA AGAGGATGAA GAAGATACAG TCGCAGATTC TGCATTTCAG
    AGCCATACCA TAGAAACAAA CTGCCAGATG AGGACCCTGG ACAGTGGGAT TGGAACCTTC
    CCACTCCCAG ACTCAGGAAC TCGTGCAGCG GGACGATACA TACGCCAAGC AGACTCCCCT
    GAGGACACTG ACCCCATCCT GTCTCTCCAA CCGGCCCTTT GTGCAGCCTC TTCCATCAGA
    GCCCAGACCC TTGAAAGAGA AGTGCCTTCC TCTGCAGATA GCCAGGGCTC TGCAGACAAT
    GCCATTGTTC ACTCCACATC CGACCCCATC ATGACTGCCA GAGGGACGCG ACCTCTTCAG
    AGTCTCCTCC CCAAACCAGC CTCCTCAGGA AAAATCAATT CCCAAATGAA GAGTGAAGCC
    GAGCCAAGGT CTCAAAATTG TTCACCATTC CAATATGTCG AAAACACAAT GGCGAGCAAG
    CCACTTCCAG CCTGGGAGGG TGATGACGCT GCTGCAGAGA CCCAGGACAA GGCACCCAGA
    ATGTGTGCAT ACTCTGCCAG CGGTAGCAGT GATAGTGACA GTGACCCGGA CTATGGAAAT
    AATGGTTTTG GAGCTGGAAG GGGCAAGTTA GTGAAAGCAA TGAAGAGCAC CACCCCAGAA
    ATTGAAACAA GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58058
    Clone ID Related Accession (Same CDS sequence) XM_015462320.1 , XM_010802114.2
    Accession Version XM_015462320.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5361bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nck-associated protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644597.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCGCTAG ATGAGGACTC GAGGAGTGAA GGGAGCAGTG CAGATGAGGG GAAAGGCAAG 
    ACCAAATGGC TGTTAGAAAG ATTGAAAGCT CTAGAGGCAG AGAATTCAGC ATTGGCTTTG
    GAAAATGAAA ATCAAAGGGA ACAGTATGAG AGATGTCTTG ATGAGGTAGC CAACCAGGTT
    GTGCAAGCCC TGCTCACTCA AAAGGATCTC AGGGAGGAAT GTGTAAAGTT GAAAACAAGA
    GTGTTTGATT TGGAACAACA GAATCGAACG CTAAGCATCC TTTTCCAACA GCGAGTCAGA
    CCAACTTCTG ATCTCCTCCT CCAGAAACTT CACTCACGCA TCTTAGATCT TTCATCCGGG
    GATTTACTCT CAGATGTGGA AGGAAGCCAG AGTCTGACGC ACTCACGAAC TGATGTTGAA
    ATGCATGGCT GCCAGCTGAA CACAAAAGCA GGAACCCCTG CTTTGAAATG CCCTGGCACG
    GGGATTGCCG TCTCTGGTCA TCTCTGCCCT CGAAGCAGCT ACAGCAGCAG TGAGCTGTCT
    CTTTCCAGCA CCTGCAGCGA GTATTCCAGC AGCTCCTCCT ACACGTGGCA TGATGGAAAG
    AACCTCAGGA AAAGGTCATC ACAAAATTGG GACAAAAGGT TAAGTATTGA CTCTTCGCTC
    CCGAGTGGCT TTGCTAGTCC TATGGATGAA CTACCTCCAA CTCGCATCAA GGAAAGCCAC
    ATTTTGGAAG GACTAAGAAA GCTCCAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG
    ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCCAATG AAGGAATCTA TTCTCCAGGA
    ATTAATAGTA ACAGCCTGAA GGAATATCCA CCCTGCAAAC CCACAGACAC AAGGAGCCCC
    TGCACGGATC CCCACAAGGC ATTTGTTTAT GACACGGATT CTCATGACGA GGCTGATGAG
    GACGCTTCCT CCTTAGCCTC TGTCCAAGCG GTTCCAAACC AGGGCTGCAG GCTGCACAGT
    TGTAAATTAA CCCACAGTGT CTCTGACAGC CTCTTTGGCT GGGAGCTGAA TGGCAAACAC
    TGTTCAGAAG TCACTTCCTC GGTCTACTCC AGGGAGAGGC CTGAGAAGCT GACCGGTTGT
    GCCAGCAACT GTCCCTTGGA GCAGAAGCTG TGCCCAGGCA TGCAGGGGCC TCGGGTACAG
    AGAGAGAAGG TCTCACCCAG CCAGGGCTGC CAGGCCCTCA GTCTCCAGCC CTCAGACACC
    GATGACCCGG AGACCCTGGA TGAGCTGCAC ATCGAGAGCA GTGATGAGAA AAGCCCTTCA
    GATGTGTCTG CAACCGCGGA CACTGACCAG TCCACGGAGA GCCTGGATAT CCTCACAGGA
    TTTGAAAAAT CTTCATGTGG GTCTCCCGAG GAGGAAGAAA ACCAAGTACC CGTCCATTTA
    GAGAGCAGGC CAAAGACGTT TAGTTTCATT AAGCAACAGA GGGTTGTAAA ACGGACTTCC
    TCAGAAGAAT GTATTACTGT GATATTTGAT GCAGAAGACG GCGAGCCCAT TGAGTTCAGC
    TCTCACCAGA CTGGCCTTGT TACTGTTACC AGAAAGGAGA TTTCCATCCA TCAGGCCCCT
    GCTGGGCCCC AGATGGAACA CACTGAACTT TCACCTCAGG GAATTGCTCA TTTACAGCCA
    GGGGCTGCAG CTAGAGACTA CCCTTTTTTA AAGAGGTCTG AAGAGGAAAC TGAAAGAAAC
    ATTCCACGAG ATGAGGTAGA TGATCCTGCC ATGACACCTG CTGTGTCTTT CCACCCCAGG
    ACAGTGACGC AAAATACTCA AAGACTGGCC AAACCGACAC ATGTCACACC ATGCCAGAGT
    CATTCTCGGT CATCTGTGGG CACAGGCGTC TGTCAGAAGA AAAGTCTGAC AAAAATACCT
    ACCAGGGGCA AGTCTTCACC TCAGAAATCG AGAGTAAGGG AGCCTGAAGC CTCCCTTATG
    ATGCCCTCAG CTGGCTCAGT GACTCTTGAG AAATCACCAG CTCCAGGGAA ACTCTCACAG
    TTCAAAAAGA CTGAAGGTCC AGCTCCCCTT TTTGATTTGC AGCCAGATTC ACACATTCCA
    AAACCCCCCA CCCAGCTTCC TCATGGCTCC AAGATGTCCA GCAGAAGGGA TTGGGTCCAG
    AGCTCCAAGA ATCAGATGCT AGCATCACAG TTGCTGTCCA GGCCCCCCAC TGAGCGGAGT
    GACGATGGGG AGCCCCCCAC GAGGGATAAA CACTGTGATC CAGGGCCAGA GGCCGGGGTG
    AAATCGCCCT CCCCACCATC CCCTCCAGGC AGGTCGGTCT CCCTACTCAT CAGGCCCAGT
    TATGACTTTT TGCCACCACC TTCATCTGCC AAATCCGAAA GCAGGGTCTC CACCGAGACA
    GCAAGAATGG TATTGAAATC ACCCCCTCTG AAAGGATCCT CTGCTCCTGT TATTTATTTT
    AACCAGACCC TGACAGATGT GCAGGGGAAG AAACCGTCTG TGGCCTTCAA AAAGCCCATC
    TTCACTCCCT CACCACCCTC CGCAGAAACA GCAATCCAGA TGAGATGCCC TGCTCACAGC
    CCCTCCAGCT CATTTGCTGT GATGGTCCCG GAGCCCCCAA AAGTCTCTCC AAAGAGAAAT
    GCCCCCAGAG CCCCACCTCA CCAAACACTT GGGACCACAC AGAACAACAC AGGGTTACAG
    ACTCCCAAGA ACTATGCTTC CCCCCGTGAG CCACTGGAAA TACCATCCTC CAAAGGTGTA
    TCTCCAGGGA GAAAAGAAAA GTTGAGTGGC AGTGTCTCTG CATCTTCCAA GCCTTCCTTC
    TTAGGGGTAA ATGAGTCACC ATCATCTCAG GTTAACAGTC CCTTATCATC AGCATCCTTC
    AAAAGCCATA ACCCCCTACA TGGTTGTCAA AACCTTCATG AGAGAGGACT GAAAACTCGC
    TTCCCAGTTG GGCTCAAAGT TTTCATGAAG TCTCCCCAGT TGCTCAGGAA AAGTTCCACG
    GTGCCAGGGA AACAGGAAAA AGACAGTCTA AATGAAGCCT CGAAAACCTC TGTGGCTGTG
    AGCAAGGCTA AGCCAGGGGC CTCCAGAAAT CCAGCCAGCC TTGAGACCAC AGGAGGGGAA
    AGAAATACGT CTCCAGTGAG TTTACCAGCA CAAGAGAGTT TGGCCAAAGG GCTTCCCCTG
    GAAAGAGCAA TGCCAGAGTC ACTGGAAAAT AGCATGCCTG GGGCTGATAG AAAAGATGGA
    GTAGAAAACA GGTCTGTAAA AAGATCCCTG TCCTCCAGCA AACCACACCT GAAACCAGCT
    CTAGGCATGA ACGGAGCCAA AGCCCGCAGC CAGAGTTTCA GTGCTCACTC AGGGGAAAAG
    CCCCCCACGC CCCCCACAGA AGGGCTGGGC AAAGTCCGGA CTCAAATTAT TACCAACACC
    GCTGAGAGAG GCAATTCCCT CACCCGGCAG AGCTCTTCTG CCGAAGGCTC TCCCAGCAAG
    AGTGCGTCTA CCCCTGTGTC TGATGGTCTC CCCAGCACTG GGAAGCCCCT GGGACACCCT
    TCCCCCAGGC AGGGTTCCTT GGGGAGCACA GGCAGCTCCA GCAGCCAGCA TGGGAGCCCA
    AGCAAGTTGC CTTTGAGGAT CCCTCCAAAA TCCGAGGAGC CCCTCACCCC AGCTGGAACA
    GAAGAGCAGC AGGCCTATGC CCAGGGAGAA GGCCCCAGGG TGACGGTACC TGAGGAACCA
    GGCAGCGACC ACTGCAGGTG CCCACCTACC CCAACAGATA GCTCAGGAGG CCCACAGAGC
    CTGGGGAGGA CGCCACATCC CAGCAGTTTC ACAGCAAGCA GGACCTCCAA GCTAGAAACT
    TCCGGAAGGT ATCCAGATAC TTCTACAACC AGGGCCGGTG TCGTAAGCCC AGAAGCCCCG
    CTCTCACCCA CAATTGAAGA AAAGGTCATG TTGTGCATTC AGGAAAATGT GGAAAAGGGC
    CAAGTGCAAA CAAAGTCCAC CTCTGTGGAA GCAAAACCGA AGCCGGGGCC TTCTTTTGCC
    AGCTGGTTCG GTTTTCGGAG GAGCAGACTC CCGGCTCTCA GTAGCAGGAA GATGGATGTC
    TCCAGAACCA AAGCAGAAAA GAAAGATGCA AAAGGTTTAG GGTTTGGAAA CAAACAGCTG
    AAATCAGAGA GAAAGAAAGA GAAAAAGAAG CCTGAGCTAC AGTGTGAGAT GGAAAATGAA
    CTTCACAGGG ATATCGAGTT GGCAGATGGT CCAGACAGTG GTTTGCAAAA CAGAAATAAC
    CCGAAAACAC CGCCAGACAT TTATGATCAA GTGAAGTTTG AATCAAGAAA TAGACCCAGC
    CCTGTTCCAT GTTCAGCGAA AGATACCTTT ATGACAGAAC TTCTGAACAG AGTTGATAAG
    AAAGCAGCTC AACAGACAGA AAGTGGATCA AATAGTCTCT CCTGTAGGAG TGTGTTAAAG
    GGCAGCTCCC AGGGCTCCTG TCTCACCAGC AGCTCTCTCA GCACTCAAGG AAACCACAAG
    AAAAACATCA AAACCAAAGC AGATATGGAA AAACCGAAAG GCTCCCTGGT AAGAGAGGCA
    AATGATCACC TGCAAGAGGA TGAAGAAGAT ACAGTCGCAG ATTCTGCATT TCAGAGCCAT
    ACCATAGAAA CAAACTGCCA GATGAGGACC CTGGACAGTG GGATTGGAAC CTTCCCACTC
    CCAGACTCAG GAACTCGTGC AGCGGGACGA TACATACGCC AAGCAGACTC CCCTGAGGAC
    ACTGACCCCA TCCTGTCTCT CCAACCGGCC CTTTGTGCAG CCTCTTCCAT CAGAGCCCAG
    ACCCTTGAAA GAGAAGTGCC TTCCTCTGCA GATAGCCAGG GCTCTGCAGA CAATGCCATT
    GTTCACTCCA CATCCGACCC CATCATGACT GCCAGAGGGA CGCGACCTCT TCAGAGTCTC
    CTCCCCAAAC CAGCCTCCTC AGGAAAAATC AATTCCCAAA TGAAGAGTGA AGCCGAGCCA
    AGGTCTCAAA ATTGTTCACC ATTCCAATAT GTCGAAAACA CAATGGCGAG CAAGCCACTT
    CCAGCCTGGG AGGGTGATGA CGCTGCTGCA GAGACCCAGA AATTGAAACA AGTTGAAGAA
    ACCAGAGAAG ATCCTGAGAA TAGATTATGC AAAATTTCCC TGGAGTCATT CAATAAATAT
    GATGGCAATA CTGTGATCTT ACTAGGAAAG GAGAAGAACT CTCTGAACAA GGTTGAAGGA
    CAAAAGGAAG AAAAAGAAAA AACTGAAGAG GCCTCTTTGA GTAGCTCTGA GAGACCTGGG
    GTAGACCACC TGGAATCTTT GAGTGATTCT TTATATGATA GCTTCTCTTC CTGTGCAAGT
    CAGGGTTCAA ATGATGTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317806.1
    CDS22..5382
    Translation

    Target ORF information:

    RefSeq Version XM_015462320.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462320.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCGCTAG ATGAGGACTC GAGGAGTGAA GGGAGCAGTG CAGATGAGGG GAAAGGCAAG 
    ACCAAATGGC TGTTAGAAAG ATTGAAAGCT CTAGAGGCAG AGAATTCAGC ATTGGCTTTG
    GAAAATGAAA ATCAAAGGGA ACAGTATGAG AGATGTCTTG ATGAGGTAGC CAACCAGGTT
    GTGCAAGCCC TGCTCACTCA AAAGGATCTC AGGGAGGAAT GTGTAAAGTT GAAAACAAGA
    GTGTTTGATT TGGAACAACA GAATCGAACG CTAAGCATCC TTTTCCAACA GCGAGTCAGA
    CCAACTTCTG ATCTCCTCCT CCAGAAACTT CACTCACGCA TCTTAGATCT TTCATCCGGG
    GATTTACTCT CAGATGTGGA AGGAAGCCAG AGTCTGACGC ACTCACGAAC TGATGTTGAA
    ATGCATGGCT GCCAGCTGAA CACAAAAGCA GGAACCCCTG CTTTGAAATG CCCTGGCACG
    GGGATTGCCG TCTCTGGTCA TCTCTGCCCT CGAAGCAGCT ACAGCAGCAG TGAGCTGTCT
    CTTTCCAGCA CCTGCAGCGA GTATTCCAGC AGCTCCTCCT ACACGTGGCA TGATGGAAAG
    AACCTCAGGA AAAGGTCATC ACAAAATTGG GACAAAAGGT TAAGTATTGA CTCTTCGCTC
    CCGAGTGGCT TTGCTAGTCC TATGGATGAA CTACCTCCAA CTCGCATCAA GGAAAGCCAC
    ATTTTGGAAG GACTAAGAAA GCTCCAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG
    ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCCAATG AAGGAATCTA TTCTCCAGGA
    ATTAATAGTA ACAGCCTGAA GGAATATCCA CCCTGCAAAC CCACAGACAC AAGGAGCCCC
    TGCACGGATC CCCACAAGGC ATTTGTTTAT GACACGGATT CTCATGACGA GGCTGATGAG
    GACGCTTCCT CCTTAGCCTC TGTCCAAGCG GTTCCAAACC AGGGCTGCAG GCTGCACAGT
    TGTAAATTAA CCCACAGTGT CTCTGACAGC CTCTTTGGCT GGGAGCTGAA TGGCAAACAC
    TGTTCAGAAG TCACTTCCTC GGTCTACTCC AGGGAGAGGC CTGAGAAGCT GACCGGTTGT
    GCCAGCAACT GTCCCTTGGA GCAGAAGCTG TGCCCAGGCA TGCAGGGGCC TCGGGTACAG
    AGAGAGAAGG TCTCACCCAG CCAGGGCTGC CAGGCCCTCA GTCTCCAGCC CTCAGACACC
    GATGACCCGG AGACCCTGGA TGAGCTGCAC ATCGAGAGCA GTGATGAGAA AAGCCCTTCA
    GATGTGTCTG CAACCGCGGA CACTGACCAG TCCACGGAGA GCCTGGATAT CCTCACAGGA
    TTTGAAAAAT CTTCATGTGG GTCTCCCGAG GAGGAAGAAA ACCAAGTACC CGTCCATTTA
    GAGAGCAGGC CAAAGACGTT TAGTTTCATT AAGCAACAGA GGGTTGTAAA ACGGACTTCC
    TCAGAAGAAT GTATTACTGT GATATTTGAT GCAGAAGACG GCGAGCCCAT TGAGTTCAGC
    TCTCACCAGA CTGGCCTTGT TACTGTTACC AGAAAGGAGA TTTCCATCCA TCAGGCCCCT
    GCTGGGCCCC AGATGGAACA CACTGAACTT TCACCTCAGG GAATTGCTCA TTTACAGCCA
    GGGGCTGCAG CTAGAGACTA CCCTTTTTTA AAGAGGTCTG AAGAGGAAAC TGAAAGAAAC
    ATTCCACGAG ATGAGGTAGA TGATCCTGCC ATGACACCTG CTGTGTCTTT CCACCCCAGG
    ACAGTGACGC AAAATACTCA AAGACTGGCC AAACCGACAC ATGTCACACC ATGCCAGAGT
    CATTCTCGGT CATCTGTGGG CACAGGCGTC TGTCAGAAGA AAAGTCTGAC AAAAATACCT
    ACCAGGGGCA AGTCTTCACC TCAGAAATCG AGAGTAAGGG AGCCTGAAGC CTCCCTTATG
    ATGCCCTCAG CTGGCTCAGT GACTCTTGAG AAATCACCAG CTCCAGGGAA ACTCTCACAG
    TTCAAAAAGA CTGAAGGTCC AGCTCCCCTT TTTGATTTGC AGCCAGATTC ACACATTCCA
    AAACCCCCCA CCCAGCTTCC TCATGGCTCC AAGATGTCCA GCAGAAGGGA TTGGGTCCAG
    AGCTCCAAGA ATCAGATGCT AGCATCACAG TTGCTGTCCA GGCCCCCCAC TGAGCGGAGT
    GACGATGGGG AGCCCCCCAC GAGGGATAAA CACTGTGATC CAGGGCCAGA GGCCGGGGTG
    AAATCGCCCT CCCCACCATC CCCTCCAGGC AGGTCGGTCT CCCTACTCAT CAGGCCCAGT
    TATGACTTTT TGCCACCACC TTCATCTGCC AAATCCGAAA GCAGGGTCTC CACCGAGACA
    GCAAGAATGG TATTGAAATC ACCCCCTCTG AAAGGATCCT CTGCTCCTGT TATTTATTTT
    AACCAGACCC TGACAGATGT GCAGGGGAAG AAACCGTCTG TGGCCTTCAA AAAGCCCATC
    TTCACTCCCT CACCACCCTC CGCAGAAACA GCAATCCAGA TGAGATGCCC TGCTCACAGC
    CCCTCCAGCT CATTTGCTGT GATGGTCCCG GAGCCCCCAA AAGTCTCTCC AAAGAGAAAT
    GCCCCCAGAG CCCCACCTCA CCAAACACTT GGGACCACAC AGAACAACAC AGGGTTACAG
    ACTCCCAAGA ACTATGCTTC CCCCCGTGAG CCACTGGAAA TACCATCCTC CAAAGGTGTA
    TCTCCAGGGA GAAAAGAAAA GTTGAGTGGC AGTGTCTCTG CATCTTCCAA GCCTTCCTTC
    TTAGGGGTAA ATGAGTCACC ATCATCTCAG GTTAACAGTC CCTTATCATC AGCATCCTTC
    AAAAGCCATA ACCCCCTACA TGGTTGTCAA AACCTTCATG AGAGAGGACT GAAAACTCGC
    TTCCCAGTTG GGCTCAAAGT TTTCATGAAG TCTCCCCAGT TGCTCAGGAA AAGTTCCACG
    GTGCCAGGGA AACAGGAAAA AGACAGTCTA AATGAAGCCT CGAAAACCTC TGTGGCTGTG
    AGCAAGGCTA AGCCAGGGGC CTCCAGAAAT CCAGCCAGCC TTGAGACCAC AGGAGGGGAA
    AGAAATACGT CTCCAGTGAG TTTACCAGCA CAAGAGAGTT TGGCCAAAGG GCTTCCCCTG
    GAAAGAGCAA TGCCAGAGTC ACTGGAAAAT AGCATGCCTG GGGCTGATAG AAAAGATGGA
    GTAGAAAACA GGTCTGTAAA AAGATCCCTG TCCTCCAGCA AACCACACCT GAAACCAGCT
    CTAGGCATGA ACGGAGCCAA AGCCCGCAGC CAGAGTTTCA GTGCTCACTC AGGGGAAAAG
    CCCCCCACGC CCCCCACAGA AGGGCTGGGC AAAGTCCGGA CTCAAATTAT TACCAACACC
    GCTGAGAGAG GCAATTCCCT CACCCGGCAG AGCTCTTCTG CCGAAGGCTC TCCCAGCAAG
    AGTGCGTCTA CCCCTGTGTC TGATGGTCTC CCCAGCACTG GGAAGCCCCT GGGACACCCT
    TCCCCCAGGC AGGGTTCCTT GGGGAGCACA GGCAGCTCCA GCAGCCAGCA TGGGAGCCCA
    AGCAAGTTGC CTTTGAGGAT CCCTCCAAAA TCCGAGGAGC CCCTCACCCC AGCTGGAACA
    GAAGAGCAGC AGGCCTATGC CCAGGGAGAA GGCCCCAGGG TGACGGTACC TGAGGAACCA
    GGCAGCGACC ACTGCAGGTG CCCACCTACC CCAACAGATA GCTCAGGAGG CCCACAGAGC
    CTGGGGAGGA CGCCACATCC CAGCAGTTTC ACAGCAAGCA GGACCTCCAA GCTAGAAACT
    TCCGGAAGGT ATCCAGATAC TTCTACAACC AGGGCCGGTG TCGTAAGCCC AGAAGCCCCG
    CTCTCACCCA CAATTGAAGA AAAGGTCATG TTGTGCATTC AGGAAAATGT GGAAAAGGGC
    CAAGTGCAAA CAAAGTCCAC CTCTGTGGAA GCAAAACCGA AGCCGGGGCC TTCTTTTGCC
    AGCTGGTTCG GTTTTCGGAG GAGCAGACTC CCGGCTCTCA GTAGCAGGAA GATGGATGTC
    TCCAGAACCA AAGCAGAAAA GAAAGATGCA AAAGGTTTAG GGTTTGGAAA CAAACAGCTG
    AAATCAGAGA GAAAGAAAGA GAAAAAGAAG CCTGAGCTAC AGTGTGAGAT GGAAAATGAA
    CTTCACAGGG ATATCGAGTT GGCAGATGGT CCAGACAGTG GTTTGCAAAA CAGAAATAAC
    CCGAAAACAC CGCCAGACAT TTATGATCAA GTGAAGTTTG AATCAAGAAA TAGACCCAGC
    CCTGTTCCAT GTTCAGCGAA AGATACCTTT ATGACAGAAC TTCTGAACAG AGTTGATAAG
    AAAGCAGCTC AACAGACAGA AAGTGGATCA AATAGTCTCT CCTGTAGGAG TGTGTTAAAG
    GGCAGCTCCC AGGGCTCCTG TCTCACCAGC AGCTCTCTCA GCACTCAAGG AAACCACAAG
    AAAAACATCA AAACCAAAGC AGATATGGAA AAACCGAAAG GCTCCCTGGT AAGAGAGGCA
    AATGATCACC TGCAAGAGGA TGAAGAAGAT ACAGTCGCAG ATTCTGCATT TCAGAGCCAT
    ACCATAGAAA CAAACTGCCA GATGAGGACC CTGGACAGTG GGATTGGAAC CTTCCCACTC
    CCAGACTCAG GAACTCGTGC AGCGGGACGA TACATACGCC AAGCAGACTC CCCTGAGGAC
    ACTGACCCCA TCCTGTCTCT CCAACCGGCC CTTTGTGCAG CCTCTTCCAT CAGAGCCCAG
    ACCCTTGAAA GAGAAGTGCC TTCCTCTGCA GATAGCCAGG GCTCTGCAGA CAATGCCATT
    GTTCACTCCA CATCCGACCC CATCATGACT GCCAGAGGGA CGCGACCTCT TCAGAGTCTC
    CTCCCCAAAC CAGCCTCCTC AGGAAAAATC AATTCCCAAA TGAAGAGTGA AGCCGAGCCA
    AGGTCTCAAA ATTGTTCACC ATTCCAATAT GTCGAAAACA CAATGGCGAG CAAGCCACTT
    CCAGCCTGGG AGGGTGATGA CGCTGCTGCA GAGACCCAGA AATTGAAACA AGTTGAAGAA
    ACCAGAGAAG ATCCTGAGAA TAGATTATGC AAAATTTCCC TGGAGTCATT CAATAAATAT
    GATGGCAATA CTGTGATCTT ACTAGGAAAG GAGAAGAACT CTCTGAACAA GGTTGAAGGA
    CAAAAGGAAG AAAAAGAAAA AACTGAAGAG GCCTCTTTGA GTAGCTCTGA GAGACCTGGG
    GTAGACCACC TGGAATCTTT GAGTGATTCT TTATATGATA GCTTCTCTTC CTGTGCAAGT
    CAGGGTTCAA ATGATGTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58058
    Clone ID Related Accession (Same CDS sequence) XM_015462320.1 , XM_010802114.2
    Accession Version XM_010802114.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5361bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nck-associated protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103841.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802114.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCGCTAG ATGAGGACTC GAGGAGTGAA GGGAGCAGTG CAGATGAGGG GAAAGGCAAG 
    ACCAAATGGC TGTTAGAAAG ATTGAAAGCT CTAGAGGCAG AGAATTCAGC ATTGGCTTTG
    GAAAATGAAA ATCAAAGGGA ACAGTATGAG AGATGTCTTG ATGAGGTAGC CAACCAGGTT
    GTGCAAGCCC TGCTCACTCA AAAGGATCTC AGGGAGGAAT GTGTAAAGTT GAAAACAAGA
    GTGTTTGATT TGGAACAACA GAATCGAACG CTAAGCATCC TTTTCCAACA GCGAGTCAGA
    CCAACTTCTG ATCTCCTCCT CCAGAAACTT CACTCACGCA TCTTAGATCT TTCATCCGGG
    GATTTACTCT CAGATGTGGA AGGAAGCCAG AGTCTGACGC ACTCACGAAC TGATGTTGAA
    ATGCATGGCT GCCAGCTGAA CACAAAAGCA GGAACCCCTG CTTTGAAATG CCCTGGCACG
    GGGATTGCCG TCTCTGGTCA TCTCTGCCCT CGAAGCAGCT ACAGCAGCAG TGAGCTGTCT
    CTTTCCAGCA CCTGCAGCGA GTATTCCAGC AGCTCCTCCT ACACGTGGCA TGATGGAAAG
    AACCTCAGGA AAAGGTCATC ACAAAATTGG GACAAAAGGT TAAGTATTGA CTCTTCGCTC
    CCGAGTGGCT TTGCTAGTCC TATGGATGAA CTACCTCCAA CTCGCATCAA GGAAAGCCAC
    ATTTTGGAAG GACTAAGAAA GCTCCAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG
    ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCCAATG AAGGAATCTA TTCTCCAGGA
    ATTAATAGTA ACAGCCTGAA GGAATATCCA CCCTGCAAAC CCACAGACAC AAGGAGCCCC
    TGCACGGATC CCCACAAGGC ATTTGTTTAT GACACGGATT CTCATGACGA GGCTGATGAG
    GACGCTTCCT CCTTAGCCTC TGTCCAAGCG GTTCCAAACC AGGGCTGCAG GCTGCACAGT
    TGTAAATTAA CCCACAGTGT CTCTGACAGC CTCTTTGGCT GGGAGCTGAA TGGCAAACAC
    TGTTCAGAAG TCACTTCCTC GGTCTACTCC AGGGAGAGGC CTGAGAAGCT GACCGGTTGT
    GCCAGCAACT GTCCCTTGGA GCAGAAGCTG TGCCCAGGCA TGCAGGGGCC TCGGGTACAG
    AGAGAGAAGG TCTCACCCAG CCAGGGCTGC CAGGCCCTCA GTCTCCAGCC CTCAGACACC
    GATGACCCGG AGACCCTGGA TGAGCTGCAC ATCGAGAGCA GTGATGAGAA AAGCCCTTCA
    GATGTGTCTG CAACCGCGGA CACTGACCAG TCCACGGAGA GCCTGGATAT CCTCACAGGA
    TTTGAAAAAT CTTCATGTGG GTCTCCCGAG GAGGAAGAAA ACCAAGTACC CGTCCATTTA
    GAGAGCAGGC CAAAGACGTT TAGTTTCATT AAGCAACAGA GGGTTGTAAA ACGGACTTCC
    TCAGAAGAAT GTATTACTGT GATATTTGAT GCAGAAGACG GCGAGCCCAT TGAGTTCAGC
    TCTCACCAGA CTGGCCTTGT TACTGTTACC AGAAAGGAGA TTTCCATCCA TCAGGCCCCT
    GCTGGGCCCC AGATGGAACA CACTGAACTT TCACCTCAGG GAATTGCTCA TTTACAGCCA
    GGGGCTGCAG CTAGAGACTA CCCTTTTTTA AAGAGGTCTG AAGAGGAAAC TGAAAGAAAC
    ATTCCACGAG ATGAGGTAGA TGATCCTGCC ATGACACCTG CTGTGTCTTT CCACCCCAGG
    ACAGTGACGC AAAATACTCA AAGACTGGCC AAACCGACAC ATGTCACACC ATGCCAGAGT
    CATTCTCGGT CATCTGTGGG CACAGGCGTC TGTCAGAAGA AAAGTCTGAC AAAAATACCT
    ACCAGGGGCA AGTCTTCACC TCAGAAATCG AGAGTAAGGG AGCCTGAAGC CTCCCTTATG
    ATGCCCTCAG CTGGCTCAGT GACTCTTGAG AAATCACCAG CTCCAGGGAA ACTCTCACAG
    TTCAAAAAGA CTGAAGGTCC AGCTCCCCTT TTTGATTTGC AGCCAGATTC ACACATTCCA
    AAACCCCCCA CCCAGCTTCC TCATGGCTCC AAGATGTCCA GCAGAAGGGA TTGGGTCCAG
    AGCTCCAAGA ATCAGATGCT AGCATCACAG TTGCTGTCCA GGCCCCCCAC TGAGCGGAGT
    GACGATGGGG AGCCCCCCAC GAGGGATAAA CACTGTGATC CAGGGCCAGA GGCCGGGGTG
    AAATCGCCCT CCCCACCATC CCCTCCAGGC AGGTCGGTCT CCCTACTCAT CAGGCCCAGT
    TATGACTTTT TGCCACCACC TTCATCTGCC AAATCCGAAA GCAGGGTCTC CACCGAGACA
    GCAAGAATGG TATTGAAATC ACCCCCTCTG AAAGGATCCT CTGCTCCTGT TATTTATTTT
    AACCAGACCC TGACAGATGT GCAGGGGAAG AAACCGTCTG TGGCCTTCAA AAAGCCCATC
    TTCACTCCCT CACCACCCTC CGCAGAAACA GCAATCCAGA TGAGATGCCC TGCTCACAGC
    CCCTCCAGCT CATTTGCTGT GATGGTCCCG GAGCCCCCAA AAGTCTCTCC AAAGAGAAAT
    GCCCCCAGAG CCCCACCTCA CCAAACACTT GGGACCACAC AGAACAACAC AGGGTTACAG
    ACTCCCAAGA ACTATGCTTC CCCCCGTGAG CCACTGGAAA TACCATCCTC CAAAGGTGTA
    TCTCCAGGGA GAAAAGAAAA GTTGAGTGGC AGTGTCTCTG CATCTTCCAA GCCTTCCTTC
    TTAGGGGTAA ATGAGTCACC ATCATCTCAG GTTAACAGTC CCTTATCATC AGCATCCTTC
    AAAAGCCATA ACCCCCTACA TGGTTGTCAA AACCTTCATG AGAGAGGACT GAAAACTCGC
    TTCCCAGTTG GGCTCAAAGT TTTCATGAAG TCTCCCCAGT TGCTCAGGAA AAGTTCCACG
    GTGCCAGGGA AACAGGAAAA AGACAGTCTA AATGAAGCCT CGAAAACCTC TGTGGCTGTG
    AGCAAGGCTA AGCCAGGGGC CTCCAGAAAT CCAGCCAGCC TTGAGACCAC AGGAGGGGAA
    AGAAATACGT CTCCAGTGAG TTTACCAGCA CAAGAGAGTT TGGCCAAAGG GCTTCCCCTG
    GAAAGAGCAA TGCCAGAGTC ACTGGAAAAT AGCATGCCTG GGGCTGATAG AAAAGATGGA
    GTAGAAAACA GGTCTGTAAA AAGATCCCTG TCCTCCAGCA AACCACACCT GAAACCAGCT
    CTAGGCATGA ACGGAGCCAA AGCCCGCAGC CAGAGTTTCA GTGCTCACTC AGGGGAAAAG
    CCCCCCACGC CCCCCACAGA AGGGCTGGGC AAAGTCCGGA CTCAAATTAT TACCAACACC
    GCTGAGAGAG GCAATTCCCT CACCCGGCAG AGCTCTTCTG CCGAAGGCTC TCCCAGCAAG
    AGTGCGTCTA CCCCTGTGTC TGATGGTCTC CCCAGCACTG GGAAGCCCCT GGGACACCCT
    TCCCCCAGGC AGGGTTCCTT GGGGAGCACA GGCAGCTCCA GCAGCCAGCA TGGGAGCCCA
    AGCAAGTTGC CTTTGAGGAT CCCTCCAAAA TCCGAGGAGC CCCTCACCCC AGCTGGAACA
    GAAGAGCAGC AGGCCTATGC CCAGGGAGAA GGCCCCAGGG TGACGGTACC TGAGGAACCA
    GGCAGCGACC ACTGCAGGTG CCCACCTACC CCAACAGATA GCTCAGGAGG CCCACAGAGC
    CTGGGGAGGA CGCCACATCC CAGCAGTTTC ACAGCAAGCA GGACCTCCAA GCTAGAAACT
    TCCGGAAGGT ATCCAGATAC TTCTACAACC AGGGCCGGTG TCGTAAGCCC AGAAGCCCCG
    CTCTCACCCA CAATTGAAGA AAAGGTCATG TTGTGCATTC AGGAAAATGT GGAAAAGGGC
    CAAGTGCAAA CAAAGTCCAC CTCTGTGGAA GCAAAACCGA AGCCGGGGCC TTCTTTTGCC
    AGCTGGTTCG GTTTTCGGAG GAGCAGACTC CCGGCTCTCA GTAGCAGGAA GATGGATGTC
    TCCAGAACCA AAGCAGAAAA GAAAGATGCA AAAGGTTTAG GGTTTGGAAA CAAACAGCTG
    AAATCAGAGA GAAAGAAAGA GAAAAAGAAG CCTGAGCTAC AGTGTGAGAT GGAAAATGAA
    CTTCACAGGG ATATCGAGTT GGCAGATGGT CCAGACAGTG GTTTGCAAAA CAGAAATAAC
    CCGAAAACAC CGCCAGACAT TTATGATCAA GTGAAGTTTG AATCAAGAAA TAGACCCAGC
    CCTGTTCCAT GTTCAGCGAA AGATACCTTT ATGACAGAAC TTCTGAACAG AGTTGATAAG
    AAAGCAGCTC AACAGACAGA AAGTGGATCA AATAGTCTCT CCTGTAGGAG TGTGTTAAAG
    GGCAGCTCCC AGGGCTCCTG TCTCACCAGC AGCTCTCTCA GCACTCAAGG AAACCACAAG
    AAAAACATCA AAACCAAAGC AGATATGGAA AAACCGAAAG GCTCCCTGGT AAGAGAGGCA
    AATGATCACC TGCAAGAGGA TGAAGAAGAT ACAGTCGCAG ATTCTGCATT TCAGAGCCAT
    ACCATAGAAA CAAACTGCCA GATGAGGACC CTGGACAGTG GGATTGGAAC CTTCCCACTC
    CCAGACTCAG GAACTCGTGC AGCGGGACGA TACATACGCC AAGCAGACTC CCCTGAGGAC
    ACTGACCCCA TCCTGTCTCT CCAACCGGCC CTTTGTGCAG CCTCTTCCAT CAGAGCCCAG
    ACCCTTGAAA GAGAAGTGCC TTCCTCTGCA GATAGCCAGG GCTCTGCAGA CAATGCCATT
    GTTCACTCCA CATCCGACCC CATCATGACT GCCAGAGGGA CGCGACCTCT TCAGAGTCTC
    CTCCCCAAAC CAGCCTCCTC AGGAAAAATC AATTCCCAAA TGAAGAGTGA AGCCGAGCCA
    AGGTCTCAAA ATTGTTCACC ATTCCAATAT GTCGAAAACA CAATGGCGAG CAAGCCACTT
    CCAGCCTGGG AGGGTGATGA CGCTGCTGCA GAGACCCAGA AATTGAAACA AGTTGAAGAA
    ACCAGAGAAG ATCCTGAGAA TAGATTATGC AAAATTTCCC TGGAGTCATT CAATAAATAT
    GATGGCAATA CTGTGATCTT ACTAGGAAAG GAGAAGAACT CTCTGAACAA GGTTGAAGGA
    CAAAAGGAAG AAAAAGAAAA AACTGAAGAG GCCTCTTTGA GTAGCTCTGA GAGACCTGGG
    GTAGACCACC TGGAATCTTT GAGTGATTCT TTATATGATA GCTTCTCTTC CTGTGCAAGT
    CAGGGTTCAA ATGATGTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800416.1
    CDS22..5382
    Translation

    Target ORF information:

    RefSeq Version XM_010802114.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NCK associated protein 5 (NCKAP5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802114.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCGCTAG ATGAGGACTC GAGGAGTGAA GGGAGCAGTG CAGATGAGGG GAAAGGCAAG 
    ACCAAATGGC TGTTAGAAAG ATTGAAAGCT CTAGAGGCAG AGAATTCAGC ATTGGCTTTG
    GAAAATGAAA ATCAAAGGGA ACAGTATGAG AGATGTCTTG ATGAGGTAGC CAACCAGGTT
    GTGCAAGCCC TGCTCACTCA AAAGGATCTC AGGGAGGAAT GTGTAAAGTT GAAAACAAGA
    GTGTTTGATT TGGAACAACA GAATCGAACG CTAAGCATCC TTTTCCAACA GCGAGTCAGA
    CCAACTTCTG ATCTCCTCCT CCAGAAACTT CACTCACGCA TCTTAGATCT TTCATCCGGG
    GATTTACTCT CAGATGTGGA AGGAAGCCAG AGTCTGACGC ACTCACGAAC TGATGTTGAA
    ATGCATGGCT GCCAGCTGAA CACAAAAGCA GGAACCCCTG CTTTGAAATG CCCTGGCACG
    GGGATTGCCG TCTCTGGTCA TCTCTGCCCT CGAAGCAGCT ACAGCAGCAG TGAGCTGTCT
    CTTTCCAGCA CCTGCAGCGA GTATTCCAGC AGCTCCTCCT ACACGTGGCA TGATGGAAAG
    AACCTCAGGA AAAGGTCATC ACAAAATTGG GACAAAAGGT TAAGTATTGA CTCTTCGCTC
    CCGAGTGGCT TTGCTAGTCC TATGGATGAA CTACCTCCAA CTCGCATCAA GGAAAGCCAC
    ATTTTGGAAG GACTAAGAAA GCTCCAGAAG CGAAAAGTGT TACTTGAACC CCCATCAGTG
    ATAACCAAAT GGGGTTATAA AGATTGCATG AACTCCAATG AAGGAATCTA TTCTCCAGGA
    ATTAATAGTA ACAGCCTGAA GGAATATCCA CCCTGCAAAC CCACAGACAC AAGGAGCCCC
    TGCACGGATC CCCACAAGGC ATTTGTTTAT GACACGGATT CTCATGACGA GGCTGATGAG
    GACGCTTCCT CCTTAGCCTC TGTCCAAGCG GTTCCAAACC AGGGCTGCAG GCTGCACAGT
    TGTAAATTAA CCCACAGTGT CTCTGACAGC CTCTTTGGCT GGGAGCTGAA TGGCAAACAC
    TGTTCAGAAG TCACTTCCTC GGTCTACTCC AGGGAGAGGC CTGAGAAGCT GACCGGTTGT
    GCCAGCAACT GTCCCTTGGA GCAGAAGCTG TGCCCAGGCA TGCAGGGGCC TCGGGTACAG
    AGAGAGAAGG TCTCACCCAG CCAGGGCTGC CAGGCCCTCA GTCTCCAGCC CTCAGACACC
    GATGACCCGG AGACCCTGGA TGAGCTGCAC ATCGAGAGCA GTGATGAGAA AAGCCCTTCA
    GATGTGTCTG CAACCGCGGA CACTGACCAG TCCACGGAGA GCCTGGATAT CCTCACAGGA
    TTTGAAAAAT CTTCATGTGG GTCTCCCGAG GAGGAAGAAA ACCAAGTACC CGTCCATTTA
    GAGAGCAGGC CAAAGACGTT TAGTTTCATT AAGCAACAGA GGGTTGTAAA ACGGACTTCC
    TCAGAAGAAT GTATTACTGT GATATTTGAT GCAGAAGACG GCGAGCCCAT TGAGTTCAGC
    TCTCACCAGA CTGGCCTTGT TACTGTTACC AGAAAGGAGA TTTCCATCCA TCAGGCCCCT
    GCTGGGCCCC AGATGGAACA CACTGAACTT TCACCTCAGG GAATTGCTCA TTTACAGCCA
    GGGGCTGCAG CTAGAGACTA CCCTTTTTTA AAGAGGTCTG AAGAGGAAAC TGAAAGAAAC
    ATTCCACGAG ATGAGGTAGA TGATCCTGCC ATGACACCTG CTGTGTCTTT CCACCCCAGG
    ACAGTGACGC AAAATACTCA AAGACTGGCC AAACCGACAC ATGTCACACC ATGCCAGAGT
    CATTCTCGGT CATCTGTGGG CACAGGCGTC TGTCAGAAGA AAAGTCTGAC AAAAATACCT
    ACCAGGGGCA AGTCTTCACC TCAGAAATCG AGAGTAAGGG AGCCTGAAGC CTCCCTTATG
    ATGCCCTCAG CTGGCTCAGT GACTCTTGAG AAATCACCAG CTCCAGGGAA ACTCTCACAG
    TTCAAAAAGA CTGAAGGTCC AGCTCCCCTT TTTGATTTGC AGCCAGATTC ACACATTCCA
    AAACCCCCCA CCCAGCTTCC TCATGGCTCC AAGATGTCCA GCAGAAGGGA TTGGGTCCAG
    AGCTCCAAGA ATCAGATGCT AGCATCACAG TTGCTGTCCA GGCCCCCCAC TGAGCGGAGT
    GACGATGGGG AGCCCCCCAC GAGGGATAAA CACTGTGATC CAGGGCCAGA GGCCGGGGTG
    AAATCGCCCT CCCCACCATC CCCTCCAGGC AGGTCGGTCT CCCTACTCAT CAGGCCCAGT
    TATGACTTTT TGCCACCACC TTCATCTGCC AAATCCGAAA GCAGGGTCTC CACCGAGACA
    GCAAGAATGG TATTGAAATC ACCCCCTCTG AAAGGATCCT CTGCTCCTGT TATTTATTTT
    AACCAGACCC TGACAGATGT GCAGGGGAAG AAACCGTCTG TGGCCTTCAA AAAGCCCATC
    TTCACTCCCT CACCACCCTC CGCAGAAACA GCAATCCAGA TGAGATGCCC TGCTCACAGC
    CCCTCCAGCT CATTTGCTGT GATGGTCCCG GAGCCCCCAA AAGTCTCTCC AAAGAGAAAT
    GCCCCCAGAG CCCCACCTCA CCAAACACTT GGGACCACAC AGAACAACAC AGGGTTACAG
    ACTCCCAAGA ACTATGCTTC CCCCCGTGAG CCACTGGAAA TACCATCCTC CAAAGGTGTA
    TCTCCAGGGA GAAAAGAAAA GTTGAGTGGC AGTGTCTCTG CATCTTCCAA GCCTTCCTTC
    TTAGGGGTAA ATGAGTCACC ATCATCTCAG GTTAACAGTC CCTTATCATC AGCATCCTTC
    AAAAGCCATA ACCCCCTACA TGGTTGTCAA AACCTTCATG AGAGAGGACT GAAAACTCGC
    TTCCCAGTTG GGCTCAAAGT TTTCATGAAG TCTCCCCAGT TGCTCAGGAA AAGTTCCACG
    GTGCCAGGGA AACAGGAAAA AGACAGTCTA AATGAAGCCT CGAAAACCTC TGTGGCTGTG
    AGCAAGGCTA AGCCAGGGGC CTCCAGAAAT CCAGCCAGCC TTGAGACCAC AGGAGGGGAA
    AGAAATACGT CTCCAGTGAG TTTACCAGCA CAAGAGAGTT TGGCCAAAGG GCTTCCCCTG
    GAAAGAGCAA TGCCAGAGTC ACTGGAAAAT AGCATGCCTG GGGCTGATAG AAAAGATGGA
    GTAGAAAACA GGTCTGTAAA AAGATCCCTG TCCTCCAGCA AACCACACCT GAAACCAGCT
    CTAGGCATGA ACGGAGCCAA AGCCCGCAGC CAGAGTTTCA GTGCTCACTC AGGGGAAAAG
    CCCCCCACGC CCCCCACAGA AGGGCTGGGC AAAGTCCGGA CTCAAATTAT TACCAACACC
    GCTGAGAGAG GCAATTCCCT CACCCGGCAG AGCTCTTCTG CCGAAGGCTC TCCCAGCAAG
    AGTGCGTCTA CCCCTGTGTC TGATGGTCTC CCCAGCACTG GGAAGCCCCT GGGACACCCT
    TCCCCCAGGC AGGGTTCCTT GGGGAGCACA GGCAGCTCCA GCAGCCAGCA TGGGAGCCCA
    AGCAAGTTGC CTTTGAGGAT CCCTCCAAAA TCCGAGGAGC CCCTCACCCC AGCTGGAACA
    GAAGAGCAGC AGGCCTATGC CCAGGGAGAA GGCCCCAGGG TGACGGTACC TGAGGAACCA
    GGCAGCGACC ACTGCAGGTG CCCACCTACC CCAACAGATA GCTCAGGAGG CCCACAGAGC
    CTGGGGAGGA CGCCACATCC CAGCAGTTTC ACAGCAAGCA GGACCTCCAA GCTAGAAACT
    TCCGGAAGGT ATCCAGATAC TTCTACAACC AGGGCCGGTG TCGTAAGCCC AGAAGCCCCG
    CTCTCACCCA CAATTGAAGA AAAGGTCATG TTGTGCATTC AGGAAAATGT GGAAAAGGGC
    CAAGTGCAAA CAAAGTCCAC CTCTGTGGAA GCAAAACCGA AGCCGGGGCC TTCTTTTGCC
    AGCTGGTTCG GTTTTCGGAG GAGCAGACTC CCGGCTCTCA GTAGCAGGAA GATGGATGTC
    TCCAGAACCA AAGCAGAAAA GAAAGATGCA AAAGGTTTAG GGTTTGGAAA CAAACAGCTG
    AAATCAGAGA GAAAGAAAGA GAAAAAGAAG CCTGAGCTAC AGTGTGAGAT GGAAAATGAA
    CTTCACAGGG ATATCGAGTT GGCAGATGGT CCAGACAGTG GTTTGCAAAA CAGAAATAAC
    CCGAAAACAC CGCCAGACAT TTATGATCAA GTGAAGTTTG AATCAAGAAA TAGACCCAGC
    CCTGTTCCAT GTTCAGCGAA AGATACCTTT ATGACAGAAC TTCTGAACAG AGTTGATAAG
    AAAGCAGCTC AACAGACAGA AAGTGGATCA AATAGTCTCT CCTGTAGGAG TGTGTTAAAG
    GGCAGCTCCC AGGGCTCCTG TCTCACCAGC AGCTCTCTCA GCACTCAAGG AAACCACAAG
    AAAAACATCA AAACCAAAGC AGATATGGAA AAACCGAAAG GCTCCCTGGT AAGAGAGGCA
    AATGATCACC TGCAAGAGGA TGAAGAAGAT ACAGTCGCAG ATTCTGCATT TCAGAGCCAT
    ACCATAGAAA CAAACTGCCA GATGAGGACC CTGGACAGTG GGATTGGAAC CTTCCCACTC
    CCAGACTCAG GAACTCGTGC AGCGGGACGA TACATACGCC AAGCAGACTC CCCTGAGGAC
    ACTGACCCCA TCCTGTCTCT CCAACCGGCC CTTTGTGCAG CCTCTTCCAT CAGAGCCCAG
    ACCCTTGAAA GAGAAGTGCC TTCCTCTGCA GATAGCCAGG GCTCTGCAGA CAATGCCATT
    GTTCACTCCA CATCCGACCC CATCATGACT GCCAGAGGGA CGCGACCTCT TCAGAGTCTC
    CTCCCCAAAC CAGCCTCCTC AGGAAAAATC AATTCCCAAA TGAAGAGTGA AGCCGAGCCA
    AGGTCTCAAA ATTGTTCACC ATTCCAATAT GTCGAAAACA CAATGGCGAG CAAGCCACTT
    CCAGCCTGGG AGGGTGATGA CGCTGCTGCA GAGACCCAGA AATTGAAACA AGTTGAAGAA
    ACCAGAGAAG ATCCTGAGAA TAGATTATGC AAAATTTCCC TGGAGTCATT CAATAAATAT
    GATGGCAATA CTGTGATCTT ACTAGGAAAG GAGAAGAACT CTCTGAACAA GGTTGAAGGA
    CAAAAGGAAG AAAAAGAAAA AACTGAAGAG GCCTCTTTGA GTAGCTCTGA GAGACCTGGG
    GTAGACCACC TGGAATCTTT GAGTGATTCT TTATATGATA GCTTCTCTTC CTGTGCAAGT
    CAGGGTTCAA ATGATGTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.