Nckap5 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006529325.3	XP_006529388.1	nck-associated protein 5 isoform X1
XM_006529330.3	XP_006529393.1	nck-associated protein 5 isoform X4
XM_006529333.3	XP_006529396.1	nck-associated protein 5 isoform X9
XM_006529334.3	XP_006529397.1	nck-associated protein 5 isoform X10
XM_030252625.1	XP_030108485.1	nck-associated protein 5 isoform X7
XM_006529335.4	XP_006529398.1	nck-associated protein 5 isoform X10
XM_030252635.1	XP_030108495.1	nck-associated protein 5 isoform X12
XM_030252637.1	XP_030108497.1	nck-associated protein 5 isoform X13
XM_011247962.2	XP_011246264.1	nck-associated protein 5 isoform X12
XM_006529329.4	XP_006529392.1	nck-associated protein 5 isoform X2
XM_006529331.4	XP_006529394.1	nck-associated protein 5 isoform X7
XM_006529333.4	XP_006529396.1	nck-associated protein 5 isoform X9
XM_011247958.2	XP_011246260.1	nck-associated protein 5 isoform X3
XM_011247960.2	XP_011246262.1	nck-associated protein 5 isoform X6
XM_006529331.3	XP_006529394.1	nck-associated protein 5 isoform X7
XM_017319542.1	XP_017175031.1	nck-associated protein 5 isoform X8
XM_006529335.3	XP_006529398.1	nck-associated protein 5 isoform X10
XM_006529336.3	XP_006529399.1	nck-associated protein 5 isoform X11
NM_001081756.1	NP_001075225.1	nck-associated protein 5 isoform 1
XM_006529325.4	XP_006529388.1	nck-associated protein 5 isoform X1
XM_006529326.4	XP_006529389.1	nck-associated protein 5 isoform X1
XM_011247958.3	XP_011246260.1	nck-associated protein 5 isoform X3
XM_006529332.3	XP_006529395.1	nck-associated protein 5 isoform X8
XM_006529326.3	XP_006529389.1	nck-associated protein 5 isoform X1
XM_006529329.3	XP_006529392.1	nck-associated protein 5 isoform X2
XM_011247963.2	XP_011246265.1	nck-associated protein 5 isoform X13
NM_172484.3	NP_766072.2	nck-associated protein 5 isoform 2
XM_011247959.2	XP_011246261.1	nck-associated protein 5 isoform X5
XM_030252628.1	XP_030108488.1	nck-associated protein 5 isoform X8
XM_006529330.4	XP_006529393.1	nck-associated protein 5 isoform X4
XM_011247960.3	XP_011246262.1	nck-associated protein 5 isoform X6
XM_006529334.4	XP_006529397.1	nck-associated protein 5 isoform X10
XM_006529336.4	XP_006529399.1	nck-associated protein 5 isoform X11
XM_011247963.3	XP_011246265.1	nck-associated protein 5 isoform X14
NM_176957.3	NP_795931.2	nck-associated protein 5 isoform 3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05781	NM_176957.3 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu03539	NM_172484.3 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$951.30 $1359.00
OMu02632	XM_006529330.4 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28056	XM_006529325.4 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28056	XM_006529326.4 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28057	XM_006529329.4 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28058	XM_006529331.4 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28058	XM_030252625.1 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28059	XM_030252628.1 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28060	XM_006529333.4 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28061	XM_006529334.4 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28061	XM_006529335.4 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28062	XM_006529336.4 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu72729	XM_011247958.3 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu72731	XM_011247960.3 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu72732	XM_030252635.1 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu72733	XM_011247963.3 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu85084	XM_030252637.1 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu02632	NM_001081756.1 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28059	XM_017319542.1 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28059	XM_006529332.3 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu72730	XM_011247959.2 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu72732	XM_011247962.2 Latest version!	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu28060	XM_006529333.3	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28058	XM_006529331.3	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28061	XM_006529335.3	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28056	XM_006529325.3	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu72729	XM_011247958.2	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28056	XM_006529326.3	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28057	XM_006529329.3	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu02632	XM_006529330.3	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu72731	XM_011247960.2	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28061	XM_006529334.3	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28062	XM_006529336.3	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu72733	XM_011247963.2	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

Nckap5 ( NM_176957.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_795931.2 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu05781
Clone ID Related Accession (Same CDS sequence)	NM_176957.3
Accession Version	NM_176957.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN700744.1, AK053243.1 and BC066095.1. On Feb 28, 2007 this sequence version replaced NM_176957.2. Transcript Variant: This variant (3) lacks several segments in the coding region, compared to variant 1, one of which results in a translational frameshift. The resulting protein (isoform 3) has a distinct C-terminus and is shorter than isoform 1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GGGGCCGAGG TCAATGAAGA TGTACAAGAA 
GATGAAGAAG ATAGAGTTGC TGACACTACT CTCCAGAGCC ACATGATAGA ATCCAACTGC 
CAGATGAGAA CACTGGACAG CGGGATTGGA ACCTTTCCCC TCCCAGACTC AGGAAACCGC 
TCAGTGGGAC GATACATATG CCAGCAGGAC TCTCCAGAGG ACACTGATGC CCTCCTGCCT 
CTCCAGCCAG CCGCTTCCAT GGCATCCTCT GCCAGAGCCC AAACCCTTGA CCGCGAAGTG 
CCTTCCTCCA CAGACAGCCA GAGCTCCCCG GAGTCCATGA TCATCCATTC CATATCTGAC 
CCCAGCATGA TGGCCAGAGA GATGAGAGCC TCACAGAGTC ACCTTCCCAA GCCTACCTCC 
TCAGGAACCG AAACAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_795931.2
CDS	377..1597
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGTETS

Target ORF information:

RefSeq Version	NM_176957.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176957.3

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GGGGCCGAGG TCAATGAAGA TGTACAAGAA 
GATGAAGAAG ATAGAGTTGC TGACACTACT CTCCAGAGCC ACATGATAGA ATCCAACTGC 
CAGATGAGAA CACTGGACAG CGGGATTGGA ACCTTTCCCC TCCCAGACTC AGGAAACCGC 
TCAGTGGGAC GATACATATG CCAGCAGGAC TCTCCAGAGG ACACTGATGC CCTCCTGCCT 
CTCCAGCCAG CCGCTTCCAT GGCATCCTCT GCCAGAGCCC AAACCCTTGA CCGCGAAGTG 
CCTTCCTCCA CAGACAGCCA GAGCTCCCCG GAGTCCATGA TCATCCATTC CATATCTGAC 
CCCAGCATGA TGGCCAGAGA GATGAGAGCC TCACAGAGTC ACCTTCCCAA GCCTACCTCC 
TCAGGAACCG AAACAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( NM_172484.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766072.2 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu03539
Clone ID Related Accession (Same CDS sequence)	NM_172484.3
Accession Version	NM_172484.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-25
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC066095.1 and AK053243.1. On Feb 28, 2007 this sequence version replaced NM_172484.2. Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream AUG and a protein (isoform 2) with a shorter N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC066095.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACGAGA AGCTGATACA TGAGTTGGAA GAGGAACGGC ACTTGCGGCT ACAGAGTGAG 
GAGCGGCTGC GGGAGGTGAC CCTGGAGTCA GAGCGCAACA GGATCCAGAT GCTCGGCCTG 
CAGCAGCAGT TTTCCAGAAT GGAAGAAACT GTCCGAAGTC TGTTGCAGAG CCAAGGATCT 
CCAGAGCAGA AAAAGGAAGA ACCTGCCAAG ATCACAGCCT ACCAGGAAGG ACTACCTGAT 
GAAGAGAGAA AAGAGCAAGC AGCACTGGGT GATCTCCATG TGGTGGCAGC TGATGAGGAC 
TCAAGAAGCG AGCACAGTAG TGTGGAGGAG GGAAAGGAAG ACAGCAGACT GCTCCTGAAG 
AGACTGAAGG CCCTGGAGGC GGAGAATTCA GCACTGGCAC TGGAAAACGA GAATCAGAGA 
GAACAGTATG AGCGATGTCT TGATGAGGTA GCCAACCAAG TTGTTCGAGC TCTACTCACT 
CAAAAGGATC TGAGGGAGGA GTGTGTAAAG TTGAAAACAA GAGTGTTTGA CTTGGAGCAA 
CAGAACCGAG CACTCAGCCT CCTGTTCCAA CAGCGAGTCA GACCAGCTTC TGATGTGCTT 
CTCCAGGAAC ACCTCCAGAA TGCAAAATCA GGAACCCCTG CCTTGAAACA CTCTGGCTCA 
GGGGTTGTCA TCCCTGATCA CCTCTGTCCT CGCAACAGCT GCAGCAGTGG CAGTGAGCTA 
TCGCTGTCAA GCACCTGCAG CGAGTACTCC AGTGGCTCCT CCTACACGTG GCAAGACAGG 
AAGCTCATTG GGAAAAGGCA ATCATCGCGG ATCTGGGATA AAAGACTAAG TATTGATTCT 
TCGCTCCCAA GTGGCTTCGC CAGCCCTGCA GATGAACTAC CTCCGACCCG TATCAAAGAA 
AGCCACATTC TAGAGGGACT GAGGAAGCTA CAGAAGCGGA AAGTGTTCCT TGAATCACCA 
TCATTGGTGA TAAGCAAATG GGGTTATAAA GATTGCATGA ACTCGAATGA AGGGATCTAT 
TCGCCAGGAA TTAAAACCAG CAGCCTCAAG GAGTGTCCGC CCTGCCAACC GTCTGGCATG 
GGGATCCCCT GCCAGAATCT CCATCAGACA TTCATCTATG ATGCAAATTC TCATGAGGAT 
GCACATGAAG AACTCCCATC CTCAGCCTTG CCACTAACAT CTCCGAGCCA GGGCTACAGA 
ATACAGGGCA GTAAATTGAC ACACAGTGTT TCAGACAGTC TGTTTAACTG GGAGCCACAT 
AGGAAGTACT TTGCAGAAGG TACAGCCTCA GTATATACCA GAGAAATGCC TGAAAGGCTA 
GCTCGTTGTG CTAGCCACTG CCCCTTGGAG AGGAAGCTAT GTCCCAGTAT ACACCTGCCC 
TGGGTGCAAA GAGACAGGGA CCCACACAGC CAAGTTCATG GCCATGTGCC TCTGAACCTG 
CAGCTCTCAG ATACCGATGA CAATGAGACC CTAAACGAGC TACACATAGA GAGCAGTGAT 
GAGAGAAGCC CTTCAGACCT GTCCTTGACT GGGGACACCG ACAAGTCCAT GGAGAACCTA 
GAGGTGGGAT TCAGAAAGTC TGAAGGTGGC TCTCGGGACA TGGGAGAAGA AGCATGTCCC 
ACCCACTTAG AGACTAGCCC GAAGGCATTC AGTTTCATCA AGCAACAAAG GGTTGTCAAG 
AGGACCTCTT CGGAAGAATG TGTCACGGTC ATATTTGATG CAGAAAATGG AGAGCCAGTG 
GAATTCAGCT CTCACCAGGC TGGAGTCGTC ACTGTCACCA GGGATGAAAT TTCCATCAGC 
TCAGCCCTTG CTGGGCCCTC AGAGGAACTC ACTGAACTTT CACCTCAGGA AATTACTCAC 
TTCCAGGCAG GAGCTACTGC AAGAGACTGT CCTTTTCTGA AGAGACCTGA AGAGGAGACT 
GCGAAAAACA TTCCAAAAGA TGAAAATCAT ATTCTCACAG CTCCGAATGA CTTCTTCAGC 
TCCCAGACTA TAATAACTCA GAATTCCCCA AGACCAAAGC TCGCCAAACC CACACAGGGG 
GTAGCCTGGC AGAGTAATTC TAGACCTGTT GGAAACATGG GTGTCTACAC AAAGCAAGGT 
CTGACAAAAA TCCCTTCCAG AGGCAAGTCT TCACCTCAGA AATCCAAACT TGTGGAGTCT 
ATGCCCACCA TGCTGGTCCC CTCCAGTGCC CTAGTGACTC CTGAAAAGAC ACCAGCATCC 
CCTATGGGGA AACTCTCACA ATGTAGAAAG GCAGAGAGCC CTGGTTCTCT CTGTGATGTA 
CAGCCAGAAT CACACATTCC AAAACACCCT GCCCAGGTTC CTCATATCCC CAAAATTTCC 
AATAAGAAGA ACTGGGTTCA GTGCCCCAGG AGTCAGACTT CAGGGATCCA GTCACGGCAT 
CTCATGGGGA GAAGTGACAG TAGTGAAATG AGGTCCAATG GCTGTGTCTC TGCGCCACAG 
ACAAGAATGA GGTCTCCCTC CCCTCCAACC CCTCCAGGGA GATCAGTGTC ACTCCTGGTT 
AGACCCAGTT ATGATTACCC ACCACTACCA TCACCTGCCA AACCTGAAAC TCAGGGCCCT 
GGAGACGCTG TAAGTCCAGG GTTGAAATCC CCGCTGTTGA AAGGATCCTC TGCTCCTCTT 
GTCTCTTCCA GTCCAGTGAT GTCAGAAATA CAGAAGAAAC CATCTGTGGC CTCCAGAAAG 
TCTGCCTTCC CTCCAGCTCT TCCCTCAACC CAAGCGGTAG TGCACACCAG ATACCCTGAA 
CACATAGCCT CCAGTCCATT TGCTGTGACG ACTTCAGGGC CCCCAAAAGT CTCTCCAAAA 
AGAGGCATCC CTAAACCTCC CCCTCACCAT GCACTAGGAA CTGCCCACAT GGACACAGAC 
TTACAGACAC CCAAGACTTG TCCTTCAAGC TGTGAACTGC TAGAGGTGAC ATCTTGTAAA 
AGCCTGTCTC CAGGGAGAAA AAGACAGTTG AGTGACAGCA CTGTGATGCC CCACAGGCCT 
TCCTTCTCAG GAGTGAATGA GTCCTTGACA CCTCAGGTTA ACAGTCCATC CTCCTCTTCC 
TCATCCTGTA GAAACCAAGG GTCCTCACAA GGTGGCCAGA ACTCTCCCGA GAAAGGACCT 
AAAACTCGCC TCCCAGTAGG ACTCAAAGTT CTCATGAAGT CTCCTCAGTT GCTCAAGAAA 
ACTTCCTTGG TACCAGGGAA ACAAGAAAAG GATAGTTTAA ATGAAGCATC TCGAAGCTGT 
GTAGCAGCAA ACACGTCTAA TCCTGAGGAC TCCAAAGACC CTGCAAGTCT GGAGACCTCA 
AGAGATGAGA GAACCACAGC CTTACTAGGT GTGAGAGGTC CCGACAGTCT AGCTAGAGGA 
CTTGCCTTAG ACTCAACAAT GCCAGAATCG TTAGAATGTA GCATCTCCAG AGATGATGGA 
AGAGATGGGG CAGACAGCAG AGCCATAAAA AGATCCTTCT CCTCCAGCAA GCCACACCTA 
AAGCCTGCTC TAGGCATGAA TGGAGCCAAA GCTCGGAGTC AGAGCTTCAG CATCCACTTG 
GGAGAGAAGC CTTCCACACC CCCAACAGAA GGGCCAGGCA AGATTCGAAC TCAGATTATC 
ACCAACACGG CTGAAAGAGG CAACTCCCTC ACCAGACAGA ATATCTCAAT GGAAGGGTCC 
CCAAGCAAGA CCCCCTCTGT CTCAACATCT GATAATCTGC CCAATGCTGG AAGGCCCCTG 
GGAAACCCCT TTTCCAGGCA GAGTTCTCTC TGTAGCACCG GAAGTTCTAG CAGCCAGCAC 
GGAAGCCCAA GCAAGTTACC CTTGAGGATG CTCACAAGGC CGGAAGGACT CCTCACTCCT 
GGAGTGGAAG ACCAACGGCT CTCTACTCAG GAAGAGTGTC CTGAAAGAGG TGTACATGAG 
CAATCTAGCA GTGACCACCA TAGGCATCCA TCTCCTCTTT CAGACTGCCC TCAGTTCCTG 
CACGTTCCAG GGAGGATGCA GCGCCCAAGC AATTTGGAAA CAAGTGGGAT ATCTAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCTTCT TCAACTGGGA CTAGTGTCGT GAGCCCAGAA 
GCCCCCCTCT CACCCTCCAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA GAATGTGGAA 
AAGGGCCAAG TGCAGACAAA GTCTGCCTCT GTGGAAGTGA AGCCCAGGGC AGGGCCTTCG 
TTCACCAGCT GGTTTGGTTT TCGAAAGAGC AGGCTTCCAG CTCTCAGTAG CAGGAGAATG 
GAAGCTTCCA AGAGTAAAGT GGAAAAGAAA GACACAAAAC TGAAATCTGA GAGGAAAAAA 
GAAAAGAAGA AAGCTGAAGT ACAATCAAAG ATAGAAAACG AACTGAGTAG GGGGACCAAG 
AAGGCAGATG GTCAGAGTCC AGACGATGGT CTGCAAAGCT CAGAGAGTCT GAAAGTATCC 
CAAGACATTT ATAACCAAAT GAAGTTGGAG CCAAGGAATA GACCCAGCCC TGCTGTGTGC 
CCAACAAAAG ATGCCTTCAT GACAGAACTT CTAAACAGAG TTGATAAGAG AGCCTCGGTA 
CAGAGAGAGA GTGGATCAAA GGGTGTTTCC TGCAGGAGCG TGTTAGAGGG CACCTCCCAG 
GGCTCCTGTT TCGCCAGCGG CTCTGTGAGC ACTCAAGGAA GTCAAAAGAA AAACATCAAA 
ACCAAAGTCG ATATGGAAAA ACCAAGAGAA TCCTTGGGGG CCGAGGTCAA TGAAGATGTA 
CAAGAAGATG AAGAAGATAG AGTTGCTGAC ACTACTCTCC AGAGCCACAT GATAGAATCC 
AACTGCCAGA TGAGAACACT GGACAGCGGG ATTGGAACCT TTCCCCTCCC AGACTCAGGA 
AACCGCTCAG TGGGACGATA CATATGCCAG CAGGACTCTC CAGAGGACAC TGATGCCCTC 
CTGCCTCTCC AGCCAGCCGC TTCCATGGCA TCCTCTGCCA GAGCCCAAAC CCTTGACCGC 
GAAGTGCCTT CCTCCACAGA CAGCCAGAGC TCCCCGGAGT CCATGATCAT CCATTCCATA 
TCTGACCCCA GCATGATGGC CAGAGAGATG AGAGCCTCAC AGAGTCACCT TCCCAAGCCT 
ACCTCCTCAG GAAAACCTAG TTTTGAAAAG CAAAATGGAG CAGAGTCAAA GGCTCACACT 
GGCCCATCAT TCGAATATGC TGAGGATGCA GTGGCAAGTG AGCCCCTTTC AGGATGGAGG 
GGTGAAGATG CTACTACTGA GACCCAGGAA CCGAAACAAG TTGAAGAAAC AAAAGAAGAT 
CCTGAGAATA GATTATCTGA AATTTCCCTG GAGTCATTCA ATAAATTTAG CAGCAATACT 
GTGATTTTAT TAGGAAAAGA GAAGTCTCCA AGTGAGGTTG AAGGACAAAA GGAAGAGAAA 
GGCAAAAAGA CAGAAGTGTC TTCGAGTAGC TCCGAACGGC CTGGGGTGGA CCGCGTGGAG 
TCTCTGAGTG ACTCTCTGTA TGACAGTTTC TCCTCTTGTG CAAGTCAAGC ATCCAACGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766072.2
CDS	442..5967
Translation	MHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	NM_172484.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172484.3

ORF Insert Sequence:

ATGCACGAGA AGCTGATACA TGAGTTGGAA GAGGAACGGC ACTTGCGGCT ACAGAGTGAG 
GAGCGGCTGC GGGAGGTGAC CCTGGAGTCA GAGCGCAACA GGATCCAGAT GCTCGGCCTG 
CAGCAGCAGT TTTCCAGAAT GGAAGAAACT GTCCGAAGTC TGTTGCAGAG CCAAGGATCT 
CCAGAGCAGA AAAAGGAAGA ACCTGCCAAG ATCACAGCCT ACCAGGAAGG ACTACCTGAT 
GAAGAGAGAA AAGAGCAAGC AGCACTGGGT GATCTCCATG TGGTGGCAGC TGATGAGGAC 
TCAAGAAGCG AGCACAGTAG TGTGGAGGAG GGAAAGGAAG ACAGCAGACT GCTCCTGAAG 
AGACTGAAGG CCCTGGAGGC GGAGAATTCA GCACTGGCAC TGGAAAACGA GAATCAGAGA 
GAACAGTATG AGCGATGTCT TGATGAGGTA GCCAACCAAG TTGTTCGAGC TCTACTCACT 
CAAAAGGATC TGAGGGAGGA GTGTGTAAAG TTGAAAACAA GAGTGTTTGA CTTGGAGCAA 
CAGAACCGAG CACTCAGCCT CCTGTTCCAA CAGCGAGTCA GACCAGCTTC TGATGTGCTT 
CTCCAGGAAC ACCTCCAGAA TGCAAAATCA GGAACCCCTG CCTTGAAACA CTCTGGCTCA 
GGGGTTGTCA TCCCTGATCA CCTCTGTCCT CGCAACAGCT GCAGCAGTGG CAGTGAGCTA 
TCGCTGTCAA GCACCTGCAG CGAGTACTCC AGTGGCTCCT CCTACACGTG GCAAGACAGG 
AAGCTCATTG GGAAAAGGCA ATCATCGCGG ATCTGGGATA AAAGACTAAG TATTGATTCT 
TCGCTCCCAA GTGGCTTCGC CAGCCCTGCA GATGAACTAC CTCCGACCCG TATCAAAGAA 
AGCCACATTC TAGAGGGACT GAGGAAGCTA CAGAAGCGGA AAGTGTTCCT TGAATCACCA 
TCATTGGTGA TAAGCAAATG GGGTTATAAA GATTGCATGA ACTCGAATGA AGGGATCTAT 
TCGCCAGGAA TTAAAACCAG CAGCCTCAAG GAGTGTCCGC CCTGCCAACC GTCTGGCATG 
GGGATCCCCT GCCAGAATCT CCATCAGACA TTCATCTATG ATGCAAATTC TCATGAGGAT 
GCACATGAAG AACTCCCATC CTCAGCCTTG CCACTAACAT CTCCGAGCCA GGGCTACAGA 
ATACAGGGCA GTAAATTGAC ACACAGTGTT TCAGACAGTC TGTTTAACTG GGAGCCACAT 
AGGAAGTACT TTGCAGAAGG TACAGCCTCA GTATATACCA GAGAAATGCC TGAAAGGCTA 
GCTCGTTGTG CTAGCCACTG CCCCTTGGAG AGGAAGCTAT GTCCCAGTAT ACACCTGCCC 
TGGGTGCAAA GAGACAGGGA CCCACACAGC CAAGTTCATG GCCATGTGCC TCTGAACCTG 
CAGCTCTCAG ATACCGATGA CAATGAGACC CTAAACGAGC TACACATAGA GAGCAGTGAT 
GAGAGAAGCC CTTCAGACCT GTCCTTGACT GGGGACACCG ACAAGTCCAT GGAGAACCTA 
GAGGTGGGAT TCAGAAAGTC TGAAGGTGGC TCTCGGGACA TGGGAGAAGA AGCATGTCCC 
ACCCACTTAG AGACTAGCCC GAAGGCATTC AGTTTCATCA AGCAACAAAG GGTTGTCAAG 
AGGACCTCTT CGGAAGAATG TGTCACGGTC ATATTTGATG CAGAAAATGG AGAGCCAGTG 
GAATTCAGCT CTCACCAGGC TGGAGTCGTC ACTGTCACCA GGGATGAAAT TTCCATCAGC 
TCAGCCCTTG CTGGGCCCTC AGAGGAACTC ACTGAACTTT CACCTCAGGA AATTACTCAC 
TTCCAGGCAG GAGCTACTGC AAGAGACTGT CCTTTTCTGA AGAGACCTGA AGAGGAGACT 
GCGAAAAACA TTCCAAAAGA TGAAAATCAT ATTCTCACAG CTCCGAATGA CTTCTTCAGC 
TCCCAGACTA TAATAACTCA GAATTCCCCA AGACCAAAGC TCGCCAAACC CACACAGGGG 
GTAGCCTGGC AGAGTAATTC TAGACCTGTT GGAAACATGG GTGTCTACAC AAAGCAAGGT 
CTGACAAAAA TCCCTTCCAG AGGCAAGTCT TCACCTCAGA AATCCAAACT TGTGGAGTCT 
ATGCCCACCA TGCTGGTCCC CTCCAGTGCC CTAGTGACTC CTGAAAAGAC ACCAGCATCC 
CCTATGGGGA AACTCTCACA ATGTAGAAAG GCAGAGAGCC CTGGTTCTCT CTGTGATGTA 
CAGCCAGAAT CACACATTCC AAAACACCCT GCCCAGGTTC CTCATATCCC CAAAATTTCC 
AATAAGAAGA ACTGGGTTCA GTGCCCCAGG AGTCAGACTT CAGGGATCCA GTCACGGCAT 
CTCATGGGGA GAAGTGACAG TAGTGAAATG AGGTCCAATG GCTGTGTCTC TGCGCCACAG 
ACAAGAATGA GGTCTCCCTC CCCTCCAACC CCTCCAGGGA GATCAGTGTC ACTCCTGGTT 
AGACCCAGTT ATGATTACCC ACCACTACCA TCACCTGCCA AACCTGAAAC TCAGGGCCCT 
GGAGACGCTG TAAGTCCAGG GTTGAAATCC CCGCTGTTGA AAGGATCCTC TGCTCCTCTT 
GTCTCTTCCA GTCCAGTGAT GTCAGAAATA CAGAAGAAAC CATCTGTGGC CTCCAGAAAG 
TCTGCCTTCC CTCCAGCTCT TCCCTCAACC CAAGCGGTAG TGCACACCAG ATACCCTGAA 
CACATAGCCT CCAGTCCATT TGCTGTGACG ACTTCAGGGC CCCCAAAAGT CTCTCCAAAA 
AGAGGCATCC CTAAACCTCC CCCTCACCAT GCACTAGGAA CTGCCCACAT GGACACAGAC 
TTACAGACAC CCAAGACTTG TCCTTCAAGC TGTGAACTGC TAGAGGTGAC ATCTTGTAAA 
AGCCTGTCTC CAGGGAGAAA AAGACAGTTG AGTGACAGCA CTGTGATGCC CCACAGGCCT 
TCCTTCTCAG GAGTGAATGA GTCCTTGACA CCTCAGGTTA ACAGTCCATC CTCCTCTTCC 
TCATCCTGTA GAAACCAAGG GTCCTCACAA GGTGGCCAGA ACTCTCCCGA GAAAGGACCT 
AAAACTCGCC TCCCAGTAGG ACTCAAAGTT CTCATGAAGT CTCCTCAGTT GCTCAAGAAA 
ACTTCCTTGG TACCAGGGAA ACAAGAAAAG GATAGTTTAA ATGAAGCATC TCGAAGCTGT 
GTAGCAGCAA ACACGTCTAA TCCTGAGGAC TCCAAAGACC CTGCAAGTCT GGAGACCTCA 
AGAGATGAGA GAACCACAGC CTTACTAGGT GTGAGAGGTC CCGACAGTCT AGCTAGAGGA 
CTTGCCTTAG ACTCAACAAT GCCAGAATCG TTAGAATGTA GCATCTCCAG AGATGATGGA 
AGAGATGGGG CAGACAGCAG AGCCATAAAA AGATCCTTCT CCTCCAGCAA GCCACACCTA 
AAGCCTGCTC TAGGCATGAA TGGAGCCAAA GCTCGGAGTC AGAGCTTCAG CATCCACTTG 
GGAGAGAAGC CTTCCACACC CCCAACAGAA GGGCCAGGCA AGATTCGAAC TCAGATTATC 
ACCAACACGG CTGAAAGAGG CAACTCCCTC ACCAGACAGA ATATCTCAAT GGAAGGGTCC 
CCAAGCAAGA CCCCCTCTGT CTCAACATCT GATAATCTGC CCAATGCTGG AAGGCCCCTG 
GGAAACCCCT TTTCCAGGCA GAGTTCTCTC TGTAGCACCG GAAGTTCTAG CAGCCAGCAC 
GGAAGCCCAA GCAAGTTACC CTTGAGGATG CTCACAAGGC CGGAAGGACT CCTCACTCCT 
GGAGTGGAAG ACCAACGGCT CTCTACTCAG GAAGAGTGTC CTGAAAGAGG TGTACATGAG 
CAATCTAGCA GTGACCACCA TAGGCATCCA TCTCCTCTTT CAGACTGCCC TCAGTTCCTG 
CACGTTCCAG GGAGGATGCA GCGCCCAAGC AATTTGGAAA CAAGTGGGAT ATCTAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCTTCT TCAACTGGGA CTAGTGTCGT GAGCCCAGAA 
GCCCCCCTCT CACCCTCCAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA GAATGTGGAA 
AAGGGCCAAG TGCAGACAAA GTCTGCCTCT GTGGAAGTGA AGCCCAGGGC AGGGCCTTCG 
TTCACCAGCT GGTTTGGTTT TCGAAAGAGC AGGCTTCCAG CTCTCAGTAG CAGGAGAATG 
GAAGCTTCCA AGAGTAAAGT GGAAAAGAAA GACACAAAAC TGAAATCTGA GAGGAAAAAA 
GAAAAGAAGA AAGCTGAAGT ACAATCAAAG ATAGAAAACG AACTGAGTAG GGGGACCAAG 
AAGGCAGATG GTCAGAGTCC AGACGATGGT CTGCAAAGCT CAGAGAGTCT GAAAGTATCC 
CAAGACATTT ATAACCAAAT GAAGTTGGAG CCAAGGAATA GACCCAGCCC TGCTGTGTGC 
CCAACAAAAG ATGCCTTCAT GACAGAACTT CTAAACAGAG TTGATAAGAG AGCCTCGGTA 
CAGAGAGAGA GTGGATCAAA GGGTGTTTCC TGCAGGAGCG TGTTAGAGGG CACCTCCCAG 
GGCTCCTGTT TCGCCAGCGG CTCTGTGAGC ACTCAAGGAA GTCAAAAGAA AAACATCAAA 
ACCAAAGTCG ATATGGAAAA ACCAAGAGAA TCCTTGGGGG CCGAGGTCAA TGAAGATGTA 
CAAGAAGATG AAGAAGATAG AGTTGCTGAC ACTACTCTCC AGAGCCACAT GATAGAATCC 
AACTGCCAGA TGAGAACACT GGACAGCGGG ATTGGAACCT TTCCCCTCCC AGACTCAGGA 
AACCGCTCAG TGGGACGATA CATATGCCAG CAGGACTCTC CAGAGGACAC TGATGCCCTC 
CTGCCTCTCC AGCCAGCCGC TTCCATGGCA TCCTCTGCCA GAGCCCAAAC CCTTGACCGC 
GAAGTGCCTT CCTCCACAGA CAGCCAGAGC TCCCCGGAGT CCATGATCAT CCATTCCATA 
TCTGACCCCA GCATGATGGC CAGAGAGATG AGAGCCTCAC AGAGTCACCT TCCCAAGCCT 
ACCTCCTCAG GAAAACCTAG TTTTGAAAAG CAAAATGGAG CAGAGTCAAA GGCTCACACT 
GGCCCATCAT TCGAATATGC TGAGGATGCA GTGGCAAGTG AGCCCCTTTC AGGATGGAGG 
GGTGAAGATG CTACTACTGA GACCCAGGAA CCGAAACAAG TTGAAGAAAC AAAAGAAGAT 
CCTGAGAATA GATTATCTGA AATTTCCCTG GAGTCATTCA ATAAATTTAG CAGCAATACT 
GTGATTTTAT TAGGAAAAGA GAAGTCTCCA AGTGAGGTTG AAGGACAAAA GGAAGAGAAA 
GGCAAAAAGA CAGAAGTGTC TTCGAGTAGC TCCGAACGGC CTGGGGTGGA CCGCGTGGAG 
TCTCTGAGTG ACTCTCTGTA TGACAGTTTC TCCTCTTGTG CAAGTCAAGC ATCCAACGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529330.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529393.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X5, mRNA.

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CloneID	OMu02632
Clone ID Related Accession (Same CDS sequence)	XM_006529330.3 , NM_001081756.1 , XM_006529330.4
Accession Version	XM_006529330.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529330.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGAACCGAAA 
CAAGTTGAAG AAACAAAAGA AGATCCTGAG AATAGATTAT CTGAAATTTC CCTGGAGTCA 
TTCAATAAAT TTAGCAGCAA TACTGTGATT TTATTAGGAA AAGAGAAGTC TCCAAGTGAG 
GTTGAAGGAC AAAAGGAAGA GAAAGGCAAA AAGACAGAAG TGTCTTCGAG TAGCTCCGAA 
CGGCCTGGGG TGGACCGCGT GGAGTCTCTG AGTGACTCTC TGTATGACAG TTTCTCCTCT 
TGTGCAAGTC AAGCATCCAA CGAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529393.1
CDS	2649..8378
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_006529330.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529330.4

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGAACCGAAA 
CAAGTTGAAG AAACAAAAGA AGATCCTGAG AATAGATTAT CTGAAATTTC CCTGGAGTCA 
TTCAATAAAT TTAGCAGCAA TACTGTGATT TTATTAGGAA AAGAGAAGTC TCCAAGTGAG 
GTTGAAGGAC AAAAGGAAGA GAAAGGCAAA AAGACAGAAG TGTCTTCGAG TAGCTCCGAA 
CGGCCTGGGG TGGACCGCGT GGAGTCTCTG AGTGACTCTC TGTATGACAG TTTCTCCTCT 
TGTGCAAGTC AAGCATCCAA CGAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529325.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529388.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X1, mRNA.

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CloneID	OMu28056
Clone ID Related Accession (Same CDS sequence)	XM_006529325.3 , XM_006529325.4 , XM_006529326.4 , XM_006529326.3
Accession Version	XM_006529325.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529325.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GAACAGGTGC 
CCTGAGGAAG CAGAAATCCT TCACAGACTT GAAAGTGACT CGAGGACAGT TAATCGTCAC 
CGTGCTCTAG TGAGGCAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529388.1
CDS	2650..8466
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_006529325.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529325.4

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GAACAGGTGC 
CCTGAGGAAG CAGAAATCCT TCACAGACTT GAAAGTGACT CGAGGACAGT TAATCGTCAC 
CGTGCTCTAG TGAGGCAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529326.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529389.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X2, mRNA.

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CloneID	OMu28056
Clone ID Related Accession (Same CDS sequence)	XM_006529325.3 , XM_006529325.4 , XM_006529326.4 , XM_006529326.3
Accession Version	XM_006529326.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529326.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GAACAGGTGC 
CCTGAGGAAG CAGAAATCCT TCACAGACTT GAAAGTGACT CGAGGACAGT TAATCGTCAC 
CGTGCTCTAG TGAGGCAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529389.1
CDS	541..6357
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_006529326.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529326.4

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GAACAGGTGC 
CCTGAGGAAG CAGAAATCCT TCACAGACTT GAAAGTGACT CGAGGACAGT TAATCGTCAC 
CGTGCTCTAG TGAGGCAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529329.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529392.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X3, mRNA.

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CloneID	OMu28057
Clone ID Related Accession (Same CDS sequence)	XM_006529329.4 , XM_006529329.3
Accession Version	XM_006529329.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529329.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGTAACCATG 
GGACATGTCA TCTTACAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529392.1
CDS	2650..8406
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQVTMGHVILQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_006529329.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529329.4

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGTAACCATG 
GGACATGTCA TCTTACAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_030252625.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108485.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X9, mRNA.

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CloneID	OMu28058
Clone ID Related Accession (Same CDS sequence)	XM_030252625.1 , XM_006529331.4 , XM_006529331.3
Accession Version	XM_030252625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG CAATCATCGC GGATCTGGGA TAAAAGACTA 
AGTATTGATT CTTCGCTCCC AAGTGGCTTC GCCAGCCCTG CAGATGAACT ACCTCCGACC 
CGTATCAAAG AAAGCCACAT TCTAGAGGGA CTGAGGAAGC TACAGAAGCG GAAAGTGTTC 
CTTGAATCAC CATCATTGGT GATAAGCAAA TGGGGTTATA AAGATTGCAT GAACTCGAAT 
GAAGGGATCT ATTCGCCAGG AATTAAAACC AGCAGCCTCA AGGAGTGTCC GCCCTGCCAA 
CCGTCTGGCA TGGGGATCCC CTGCCAGAAT CTCCATCAGA CATTCATCTA TGATGCAAAT 
TCTCATGAGG ATGCACATGA AGAACTCCCA TCCTCAGCCT TGCCACTAAC ATCTCCGAGC 
CAGGGCTACA GAATACAGGG CAGTAAATTG ACACACAGTG TTTCAGACAG TCTGTTTAAC 
TGGGAGCCAC ATAGGAAGTA CTTTGCAGAA GGTACAGCCT CAGTATATAC CAGAGAAATG 
CCTGAAAGGC TAGCTCGTTG TGCTAGCCAC TGCCCCTTGG AGAGGAAGCT ATGTCCCAGT 
ATACACCTGC CCTGGGTGCA AAGAGACAGG GACCCACACA GCCAAGTTCA TGGCCATGTG 
CCTCTGAACC TGCAGCTCTC AGATACCGAT GACAATGAGA CCCTAAACGA GCTACACATA 
GAGAGCAGTG ATGAGAGAAG CCCTTCAGAC CTGTCCTTGA CTGGGGACAC CGACAAGTCC 
ATGGAGAACC TAGAGGTGGG ATTCAGAAAG TCTGAAGGTG GCTCTCGGGA CATGGGAGAA 
GAAGCATGTC CCACCCACTT AGAGACTAGC CCGAAGGCAT TCAGTTTCAT CAAGCAACAA 
AGGGTTGTCA AGAGGACCTC TTCGGAAGAA TGTGTCACGG TCATATTTGA TGCAGAAAAT 
GGAGAGCCAG TGGAATTCAG CTCTCACCAG GCTGGAGTCG TCACTGTCAC CAGGGATGAA 
ATTTCCATCA GCTCAGCCCT TGCTGGGCCC TCAGAGGAAC TCACTGAACT TTCACCTCAG 
GAAATTACTC ACTTCCAGGC AGGAGCTACT GCAAGAGACT GTCCTTTTCT GAAGAGACCT 
GAAGAGGAGA CTGCGAAAAA CATTCCAAAA GATGAAAATC ATATTCTCAC AGCTCCGAAT 
GACTTCTTCA GCTCCCAGAC TATAATAACT CAGAATTCCC CAAGACCAAA GCTCGCCAAA 
CCCACACAGG GGGTAGCCTG GCAGAGTAAT TCTAGACCTG TTGGAAACAT GGGTGTCTAC 
ACAAAGCAAG GTCTGACAAA AATCCCTTCC AGAGGCAAGT CTTCACCTCA GAAATCCAAA 
CTTGTGGAGT CTATGCCCAC CATGCTGGTC CCCTCCAGTG CCCTAGTGAC TCCTGAAAAG 
ACACCAGCAT CCCCTATGGG GAAACTCTCA CAATGTAGAA AGGCAGAGAG CCCTGGTTCT 
CTCTGTGATG TACAGCCAGA ATCACACATT CCAAAACACC CTGCCCAGGT TCCTCATATC 
CCCAAAATTT CCAATAAGAA GAACTGGGTT CAGTGCCCCA GGAGTCAGAC TTCAGGGATC 
CAGTCACGGC ATCTCATGGG GAGAAGTGAC AGTAGTGAAA TGAGGTCCAA TGGCTGTGTC 
TCTGCGCCAC AGACAAGAAT GAGGTCTCCC TCCCCTCCAA CCCCTCCAGG GAGATCAGTG 
TCACTCCTGG TTAGACCCAG TTATGATTAC CCACCACTAC CATCACCTGC CAAACCTGAA 
ACTCAGGGCC CTGGAGACGC TGTAAGTCCA GGGTTGAAAT CCCCGCTGTT GAAAGGATCC 
TCTGCTCCTC TTGTCTCTTC CAGTCCAGTG ATGTCAGAAA TACAGAAGAA ACCATCTGTG 
GCCTCCAGAA AGTCTGCCTT CCCTCCAGCT CTTCCCTCAA CCCAAGCGGT AGTGCACACC 
AGATACCCTG AACACATAGC CTCCAGTCCA TTTGCTGTGA CGACTTCAGG GCCCCCAAAA 
GTCTCTCCAA AAAGAGGCAT CCCTAAACCT CCCCCTCACC ATGCACTAGG AACTGCCCAC 
ATGGACACAG ACTTACAGAC ACCCAAGACT TGTCCTTCAA GCTGTGAACT GCTAGAGGTG 
ACATCTTGTA AAAGCCTGTC TCCAGGGAGA AAAAGACAGT TGAGTGACAG CACTGTGATG 
CCCCACAGGC CTTCCTTCTC AGGAGTGAAT GAGTCCTTGA CACCTCAGGT TAACAGTCCA 
TCCTCCTCTT CCTCATCCTG TAGAAACCAA GGGTCCTCAC AAGGTGGCCA GAACTCTCCC 
GAGAAAGGAC CTAAAACTCG CCTCCCAGTA GGACTCAAAG TTCTCATGAA GTCTCCTCAG 
TTGCTCAAGA AAACTTCCTT GGTACCAGGG AAACAAGAAA AGGATAGTTT AAATGAAGCA 
TCTCGAAGCT GTGTAGCAGC AAACACGTCT AATCCTGAGG ACTCCAAAGA CCCTGCAAGT 
CTGGAGACCT CAAGAGATGA GAGAACCACA GCCTTACTAG GTGTGAGAGG TCCCGACAGT 
CTAGCTAGAG GACTTGCCTT AGACTCAACA ATGCCAGAAT CGTTAGAATG TAGCATCTCC 
AGAGATGATG GAAGAGATGG GGCAGACAGC AGAGCCATAA AAAGATCCTT CTCCTCCAGC 
AAGCCACACC TAAAGCCTGC TCTAGGCATG AATGGAGCCA AAGCTCGGAG TCAGAGCTTC 
AGCATCCACT TGGGAGAGAA GCCTTCCACA CCCCCAACAG AAGGGCCAGG CAAGATTCGA 
ACTCAGATTA TCACCAACAC GGCTGAAAGA GGCAACTCCC TCACCAGACA GAATATCTCA 
ATGGAAGGGT CCCCAAGCAA GACCCCCTCT GTCTCAACAT CTGATAATCT GCCCAATGCT 
GGAAGGCCCC TGGGAAACCC CTTTTCCAGG CAGAGTTCTC TCTGTAGCAC CGGAAGTTCT 
AGCAGCCAGC ACGGAAGCCC AAGCAAGTTA CCCTTGAGGA TGCTCACAAG GCCGGAAGGA 
CTCCTCACTC CTGGAGTGGA AGACCAACGG CTCTCTACTC AGGAAGAGTG TCCTGAAAGA 
GGTGTACATG AGCAATCTAG CAGTGACCAC CATAGGCATC CATCTCCTCT TTCAGACTGC 
CCTCAGTTCC TGCACGTTCC AGGGAGGATG CAGCGCCCAA GCAATTTGGA AACAAGTGGG 
ATATCTAAGC TAGAAACTTC TGGAAGGCAT CCAGATGCTT CTTCAACTGG GACTAGTGTC 
GTGAGCCCAG AAGCCCCCCT CTCACCCTCC ATCGAAGAAA AGGTCATGTT GTGCATTCAG 
GAGAATGTGG AAAAGGGCCA AGTGCAGACA AAGTCTGCCT CTGTGGAAGT GAAGCCCAGG 
GCAGGGCCTT CGTTCACCAG CTGGTTTGGT TTTCGAAAGA GCAGGCTTCC AGCTCTCAGT 
AGCAGGAGAA TGGAAGCTTC CAAGAGTAAA GTGGAAAAGA AAGACACAAA ACTGAAATCT 
GAGAGGAAAA AAGAAAAGAA GAAAGCTGAA GTACAATCAA AGATAGAAAA CGAACTGAGT 
AGGGGGACCA AGAAGGCAGA TGGTCAGAGT CCAGACGATG GTCTGCAAAG CTCAGAGAGT 
CTGAAAGTAT CCCAAGACAT TTATAACCAA ATGAAGTTGG AGCCAAGGAA TAGACCCAGC 
CCTGCTGTGT GCCCAACAAA AGATGCCTTC ATGACAGAAC TTCTAAACAG AGTTGATAAG 
AGAGCCTCGG TACAGAGAGA GAGTGGATCA AAGGGTGTTT CCTGCAGGAG CGTGTTAGAG 
GGCACCTCCC AGGGCTCCTG TTTCGCCAGC GGCTCTGTGA GCACTCAAGG AAGTCAAAAG 
AAAAACATCA AAACCAAAGT CGATATGGAA AAACCAAGAG AATCCTTGGG GGCCGAGGTC 
AATGAAGATG TACAAGAAGA TGAAGAAGAT AGAGTTGCTG ACACTACTCT CCAGAGCCAC 
ATGATAGAAT CCAACTGCCA GATGAGAACA CTGGACAGCG GGATTGGAAC CTTTCCCCTC 
CCAGACTCAG GAAACCGCTC AGTGGGACGA TACATATGCC AGCAGGACTC TCCAGAGGAC 
ACTGATGCCC TCCTGCCTCT CCAGCCAGCC GCTTCCATGG CATCCTCTGC CAGAGCCCAA 
ACCCTTGACC GCGAAGTGCC TTCCTCCACA GACAGCCAGA GCTCCCCGGA GTCCATGATC 
ATCCATTCCA TATCTGACCC CAGCATGATG GCCAGAGAGA TGAGAGCCTC ACAGAGTCAC 
CTTCCCAAGC CTACCTCCTC AGGAAAACCT AGTTTTGAAA AGCAAAATGG AGCAGAGTCA 
AAGGCTCACA CTGGCCCATC ATTCGAATAT GCTGAGGATG CAGTGGCAAG TGAGCCCCTT 
TCAGGATGGA GGGGTGAAGA TGCTACTACT GAGACCCAGA ACAGGTGCCC TGAGGAAGCA 
GAAATCCTTC ACAGACTTGA AAGTGACTCG AGGACAGTTA ATCGTCACCG TGCTCTAGTG 
AGGCAGGAAC CGAAACAAGT TGAAGAAACA AAAGAAGATC CTGAGAATAG ATTATCTGAA 
ATTTCCCTGG AGTCATTCAA TAAATTTAGC AGCAATACTG TGATTTTATT AGGAAAAGAG 
AAGTCTCCAA GTGAGGTTGA AGGACAAAAG GAAGAGAAAG GCAAAAAGAC AGAAGTGTCT 
TCGAGTAGCT CCGAACGGCC TGGGGTGGAC CGCGTGGAGT CTCTGAGTGA CTCTCTGTAT 
GACAGTTTCT CCTCTTGTGC AAGTCAAGCA TCCAACGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108485.1
CDS	541..6165
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_030252625.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252625.1

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG CAATCATCGC GGATCTGGGA TAAAAGACTA 
AGTATTGATT CTTCGCTCCC AAGTGGCTTC GCCAGCCCTG CAGATGAACT ACCTCCGACC 
CGTATCAAAG AAAGCCACAT TCTAGAGGGA CTGAGGAAGC TACAGAAGCG GAAAGTGTTC 
CTTGAATCAC CATCATTGGT GATAAGCAAA TGGGGTTATA AAGATTGCAT GAACTCGAAT 
GAAGGGATCT ATTCGCCAGG AATTAAAACC AGCAGCCTCA AGGAGTGTCC GCCCTGCCAA 
CCGTCTGGCA TGGGGATCCC CTGCCAGAAT CTCCATCAGA CATTCATCTA TGATGCAAAT 
TCTCATGAGG ATGCACATGA AGAACTCCCA TCCTCAGCCT TGCCACTAAC ATCTCCGAGC 
CAGGGCTACA GAATACAGGG CAGTAAATTG ACACACAGTG TTTCAGACAG TCTGTTTAAC 
TGGGAGCCAC ATAGGAAGTA CTTTGCAGAA GGTACAGCCT CAGTATATAC CAGAGAAATG 
CCTGAAAGGC TAGCTCGTTG TGCTAGCCAC TGCCCCTTGG AGAGGAAGCT ATGTCCCAGT 
ATACACCTGC CCTGGGTGCA AAGAGACAGG GACCCACACA GCCAAGTTCA TGGCCATGTG 
CCTCTGAACC TGCAGCTCTC AGATACCGAT GACAATGAGA CCCTAAACGA GCTACACATA 
GAGAGCAGTG ATGAGAGAAG CCCTTCAGAC CTGTCCTTGA CTGGGGACAC CGACAAGTCC 
ATGGAGAACC TAGAGGTGGG ATTCAGAAAG TCTGAAGGTG GCTCTCGGGA CATGGGAGAA 
GAAGCATGTC CCACCCACTT AGAGACTAGC CCGAAGGCAT TCAGTTTCAT CAAGCAACAA 
AGGGTTGTCA AGAGGACCTC TTCGGAAGAA TGTGTCACGG TCATATTTGA TGCAGAAAAT 
GGAGAGCCAG TGGAATTCAG CTCTCACCAG GCTGGAGTCG TCACTGTCAC CAGGGATGAA 
ATTTCCATCA GCTCAGCCCT TGCTGGGCCC TCAGAGGAAC TCACTGAACT TTCACCTCAG 
GAAATTACTC ACTTCCAGGC AGGAGCTACT GCAAGAGACT GTCCTTTTCT GAAGAGACCT 
GAAGAGGAGA CTGCGAAAAA CATTCCAAAA GATGAAAATC ATATTCTCAC AGCTCCGAAT 
GACTTCTTCA GCTCCCAGAC TATAATAACT CAGAATTCCC CAAGACCAAA GCTCGCCAAA 
CCCACACAGG GGGTAGCCTG GCAGAGTAAT TCTAGACCTG TTGGAAACAT GGGTGTCTAC 
ACAAAGCAAG GTCTGACAAA AATCCCTTCC AGAGGCAAGT CTTCACCTCA GAAATCCAAA 
CTTGTGGAGT CTATGCCCAC CATGCTGGTC CCCTCCAGTG CCCTAGTGAC TCCTGAAAAG 
ACACCAGCAT CCCCTATGGG GAAACTCTCA CAATGTAGAA AGGCAGAGAG CCCTGGTTCT 
CTCTGTGATG TACAGCCAGA ATCACACATT CCAAAACACC CTGCCCAGGT TCCTCATATC 
CCCAAAATTT CCAATAAGAA GAACTGGGTT CAGTGCCCCA GGAGTCAGAC TTCAGGGATC 
CAGTCACGGC ATCTCATGGG GAGAAGTGAC AGTAGTGAAA TGAGGTCCAA TGGCTGTGTC 
TCTGCGCCAC AGACAAGAAT GAGGTCTCCC TCCCCTCCAA CCCCTCCAGG GAGATCAGTG 
TCACTCCTGG TTAGACCCAG TTATGATTAC CCACCACTAC CATCACCTGC CAAACCTGAA 
ACTCAGGGCC CTGGAGACGC TGTAAGTCCA GGGTTGAAAT CCCCGCTGTT GAAAGGATCC 
TCTGCTCCTC TTGTCTCTTC CAGTCCAGTG ATGTCAGAAA TACAGAAGAA ACCATCTGTG 
GCCTCCAGAA AGTCTGCCTT CCCTCCAGCT CTTCCCTCAA CCCAAGCGGT AGTGCACACC 
AGATACCCTG AACACATAGC CTCCAGTCCA TTTGCTGTGA CGACTTCAGG GCCCCCAAAA 
GTCTCTCCAA AAAGAGGCAT CCCTAAACCT CCCCCTCACC ATGCACTAGG AACTGCCCAC 
ATGGACACAG ACTTACAGAC ACCCAAGACT TGTCCTTCAA GCTGTGAACT GCTAGAGGTG 
ACATCTTGTA AAAGCCTGTC TCCAGGGAGA AAAAGACAGT TGAGTGACAG CACTGTGATG 
CCCCACAGGC CTTCCTTCTC AGGAGTGAAT GAGTCCTTGA CACCTCAGGT TAACAGTCCA 
TCCTCCTCTT CCTCATCCTG TAGAAACCAA GGGTCCTCAC AAGGTGGCCA GAACTCTCCC 
GAGAAAGGAC CTAAAACTCG CCTCCCAGTA GGACTCAAAG TTCTCATGAA GTCTCCTCAG 
TTGCTCAAGA AAACTTCCTT GGTACCAGGG AAACAAGAAA AGGATAGTTT AAATGAAGCA 
TCTCGAAGCT GTGTAGCAGC AAACACGTCT AATCCTGAGG ACTCCAAAGA CCCTGCAAGT 
CTGGAGACCT CAAGAGATGA GAGAACCACA GCCTTACTAG GTGTGAGAGG TCCCGACAGT 
CTAGCTAGAG GACTTGCCTT AGACTCAACA ATGCCAGAAT CGTTAGAATG TAGCATCTCC 
AGAGATGATG GAAGAGATGG GGCAGACAGC AGAGCCATAA AAAGATCCTT CTCCTCCAGC 
AAGCCACACC TAAAGCCTGC TCTAGGCATG AATGGAGCCA AAGCTCGGAG TCAGAGCTTC 
AGCATCCACT TGGGAGAGAA GCCTTCCACA CCCCCAACAG AAGGGCCAGG CAAGATTCGA 
ACTCAGATTA TCACCAACAC GGCTGAAAGA GGCAACTCCC TCACCAGACA GAATATCTCA 
ATGGAAGGGT CCCCAAGCAA GACCCCCTCT GTCTCAACAT CTGATAATCT GCCCAATGCT 
GGAAGGCCCC TGGGAAACCC CTTTTCCAGG CAGAGTTCTC TCTGTAGCAC CGGAAGTTCT 
AGCAGCCAGC ACGGAAGCCC AAGCAAGTTA CCCTTGAGGA TGCTCACAAG GCCGGAAGGA 
CTCCTCACTC CTGGAGTGGA AGACCAACGG CTCTCTACTC AGGAAGAGTG TCCTGAAAGA 
GGTGTACATG AGCAATCTAG CAGTGACCAC CATAGGCATC CATCTCCTCT TTCAGACTGC 
CCTCAGTTCC TGCACGTTCC AGGGAGGATG CAGCGCCCAA GCAATTTGGA AACAAGTGGG 
ATATCTAAGC TAGAAACTTC TGGAAGGCAT CCAGATGCTT CTTCAACTGG GACTAGTGTC 
GTGAGCCCAG AAGCCCCCCT CTCACCCTCC ATCGAAGAAA AGGTCATGTT GTGCATTCAG 
GAGAATGTGG AAAAGGGCCA AGTGCAGACA AAGTCTGCCT CTGTGGAAGT GAAGCCCAGG 
GCAGGGCCTT CGTTCACCAG CTGGTTTGGT TTTCGAAAGA GCAGGCTTCC AGCTCTCAGT 
AGCAGGAGAA TGGAAGCTTC CAAGAGTAAA GTGGAAAAGA AAGACACAAA ACTGAAATCT 
GAGAGGAAAA AAGAAAAGAA GAAAGCTGAA GTACAATCAA AGATAGAAAA CGAACTGAGT 
AGGGGGACCA AGAAGGCAGA TGGTCAGAGT CCAGACGATG GTCTGCAAAG CTCAGAGAGT 
CTGAAAGTAT CCCAAGACAT TTATAACCAA ATGAAGTTGG AGCCAAGGAA TAGACCCAGC 
CCTGCTGTGT GCCCAACAAA AGATGCCTTC ATGACAGAAC TTCTAAACAG AGTTGATAAG 
AGAGCCTCGG TACAGAGAGA GAGTGGATCA AAGGGTGTTT CCTGCAGGAG CGTGTTAGAG 
GGCACCTCCC AGGGCTCCTG TTTCGCCAGC GGCTCTGTGA GCACTCAAGG AAGTCAAAAG 
AAAAACATCA AAACCAAAGT CGATATGGAA AAACCAAGAG AATCCTTGGG GGCCGAGGTC 
AATGAAGATG TACAAGAAGA TGAAGAAGAT AGAGTTGCTG ACACTACTCT CCAGAGCCAC 
ATGATAGAAT CCAACTGCCA GATGAGAACA CTGGACAGCG GGATTGGAAC CTTTCCCCTC 
CCAGACTCAG GAAACCGCTC AGTGGGACGA TACATATGCC AGCAGGACTC TCCAGAGGAC 
ACTGATGCCC TCCTGCCTCT CCAGCCAGCC GCTTCCATGG CATCCTCTGC CAGAGCCCAA 
ACCCTTGACC GCGAAGTGCC TTCCTCCACA GACAGCCAGA GCTCCCCGGA GTCCATGATC 
ATCCATTCCA TATCTGACCC CAGCATGATG GCCAGAGAGA TGAGAGCCTC ACAGAGTCAC 
CTTCCCAAGC CTACCTCCTC AGGAAAACCT AGTTTTGAAA AGCAAAATGG AGCAGAGTCA 
AAGGCTCACA CTGGCCCATC ATTCGAATAT GCTGAGGATG CAGTGGCAAG TGAGCCCCTT 
TCAGGATGGA GGGGTGAAGA TGCTACTACT GAGACCCAGA ACAGGTGCCC TGAGGAAGCA 
GAAATCCTTC ACAGACTTGA AAGTGACTCG AGGACAGTTA ATCGTCACCG TGCTCTAGTG 
AGGCAGGAAC CGAAACAAGT TGAAGAAACA AAAGAAGATC CTGAGAATAG ATTATCTGAA 
ATTTCCCTGG AGTCATTCAA TAAATTTAGC AGCAATACTG TGATTTTATT AGGAAAAGAG 
AAGTCTCCAA GTGAGGTTGA AGGACAAAAG GAAGAGAAAG GCAAAAAGAC AGAAGTGTCT 
TCGAGTAGCT CCGAACGGCC TGGGGTGGAC CGCGTGGAGT CTCTGAGTGA CTCTCTGTAT 
GACAGTTTCT CCTCTTGTGC AAGTCAAGCA TCCAACGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529331.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529394.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X8, mRNA.

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CloneID	OMu28058
Clone ID Related Accession (Same CDS sequence)	XM_030252625.1 , XM_006529331.4 , XM_006529331.3
Accession Version	XM_006529331.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529331.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG CAATCATCGC GGATCTGGGA TAAAAGACTA 
AGTATTGATT CTTCGCTCCC AAGTGGCTTC GCCAGCCCTG CAGATGAACT ACCTCCGACC 
CGTATCAAAG AAAGCCACAT TCTAGAGGGA CTGAGGAAGC TACAGAAGCG GAAAGTGTTC 
CTTGAATCAC CATCATTGGT GATAAGCAAA TGGGGTTATA AAGATTGCAT GAACTCGAAT 
GAAGGGATCT ATTCGCCAGG AATTAAAACC AGCAGCCTCA AGGAGTGTCC GCCCTGCCAA 
CCGTCTGGCA TGGGGATCCC CTGCCAGAAT CTCCATCAGA CATTCATCTA TGATGCAAAT 
TCTCATGAGG ATGCACATGA AGAACTCCCA TCCTCAGCCT TGCCACTAAC ATCTCCGAGC 
CAGGGCTACA GAATACAGGG CAGTAAATTG ACACACAGTG TTTCAGACAG TCTGTTTAAC 
TGGGAGCCAC ATAGGAAGTA CTTTGCAGAA GGTACAGCCT CAGTATATAC CAGAGAAATG 
CCTGAAAGGC TAGCTCGTTG TGCTAGCCAC TGCCCCTTGG AGAGGAAGCT ATGTCCCAGT 
ATACACCTGC CCTGGGTGCA AAGAGACAGG GACCCACACA GCCAAGTTCA TGGCCATGTG 
CCTCTGAACC TGCAGCTCTC AGATACCGAT GACAATGAGA CCCTAAACGA GCTACACATA 
GAGAGCAGTG ATGAGAGAAG CCCTTCAGAC CTGTCCTTGA CTGGGGACAC CGACAAGTCC 
ATGGAGAACC TAGAGGTGGG ATTCAGAAAG TCTGAAGGTG GCTCTCGGGA CATGGGAGAA 
GAAGCATGTC CCACCCACTT AGAGACTAGC CCGAAGGCAT TCAGTTTCAT CAAGCAACAA 
AGGGTTGTCA AGAGGACCTC TTCGGAAGAA TGTGTCACGG TCATATTTGA TGCAGAAAAT 
GGAGAGCCAG TGGAATTCAG CTCTCACCAG GCTGGAGTCG TCACTGTCAC CAGGGATGAA 
ATTTCCATCA GCTCAGCCCT TGCTGGGCCC TCAGAGGAAC TCACTGAACT TTCACCTCAG 
GAAATTACTC ACTTCCAGGC AGGAGCTACT GCAAGAGACT GTCCTTTTCT GAAGAGACCT 
GAAGAGGAGA CTGCGAAAAA CATTCCAAAA GATGAAAATC ATATTCTCAC AGCTCCGAAT 
GACTTCTTCA GCTCCCAGAC TATAATAACT CAGAATTCCC CAAGACCAAA GCTCGCCAAA 
CCCACACAGG GGGTAGCCTG GCAGAGTAAT TCTAGACCTG TTGGAAACAT GGGTGTCTAC 
ACAAAGCAAG GTCTGACAAA AATCCCTTCC AGAGGCAAGT CTTCACCTCA GAAATCCAAA 
CTTGTGGAGT CTATGCCCAC CATGCTGGTC CCCTCCAGTG CCCTAGTGAC TCCTGAAAAG 
ACACCAGCAT CCCCTATGGG GAAACTCTCA CAATGTAGAA AGGCAGAGAG CCCTGGTTCT 
CTCTGTGATG TACAGCCAGA ATCACACATT CCAAAACACC CTGCCCAGGT TCCTCATATC 
CCCAAAATTT CCAATAAGAA GAACTGGGTT CAGTGCCCCA GGAGTCAGAC TTCAGGGATC 
CAGTCACGGC ATCTCATGGG GAGAAGTGAC AGTAGTGAAA TGAGGTCCAA TGGCTGTGTC 
TCTGCGCCAC AGACAAGAAT GAGGTCTCCC TCCCCTCCAA CCCCTCCAGG GAGATCAGTG 
TCACTCCTGG TTAGACCCAG TTATGATTAC CCACCACTAC CATCACCTGC CAAACCTGAA 
ACTCAGGGCC CTGGAGACGC TGTAAGTCCA GGGTTGAAAT CCCCGCTGTT GAAAGGATCC 
TCTGCTCCTC TTGTCTCTTC CAGTCCAGTG ATGTCAGAAA TACAGAAGAA ACCATCTGTG 
GCCTCCAGAA AGTCTGCCTT CCCTCCAGCT CTTCCCTCAA CCCAAGCGGT AGTGCACACC 
AGATACCCTG AACACATAGC CTCCAGTCCA TTTGCTGTGA CGACTTCAGG GCCCCCAAAA 
GTCTCTCCAA AAAGAGGCAT CCCTAAACCT CCCCCTCACC ATGCACTAGG AACTGCCCAC 
ATGGACACAG ACTTACAGAC ACCCAAGACT TGTCCTTCAA GCTGTGAACT GCTAGAGGTG 
ACATCTTGTA AAAGCCTGTC TCCAGGGAGA AAAAGACAGT TGAGTGACAG CACTGTGATG 
CCCCACAGGC CTTCCTTCTC AGGAGTGAAT GAGTCCTTGA CACCTCAGGT TAACAGTCCA 
TCCTCCTCTT CCTCATCCTG TAGAAACCAA GGGTCCTCAC AAGGTGGCCA GAACTCTCCC 
GAGAAAGGAC CTAAAACTCG CCTCCCAGTA GGACTCAAAG TTCTCATGAA GTCTCCTCAG 
TTGCTCAAGA AAACTTCCTT GGTACCAGGG AAACAAGAAA AGGATAGTTT AAATGAAGCA 
TCTCGAAGCT GTGTAGCAGC AAACACGTCT AATCCTGAGG ACTCCAAAGA CCCTGCAAGT 
CTGGAGACCT CAAGAGATGA GAGAACCACA GCCTTACTAG GTGTGAGAGG TCCCGACAGT 
CTAGCTAGAG GACTTGCCTT AGACTCAACA ATGCCAGAAT CGTTAGAATG TAGCATCTCC 
AGAGATGATG GAAGAGATGG GGCAGACAGC AGAGCCATAA AAAGATCCTT CTCCTCCAGC 
AAGCCACACC TAAAGCCTGC TCTAGGCATG AATGGAGCCA AAGCTCGGAG TCAGAGCTTC 
AGCATCCACT TGGGAGAGAA GCCTTCCACA CCCCCAACAG AAGGGCCAGG CAAGATTCGA 
ACTCAGATTA TCACCAACAC GGCTGAAAGA GGCAACTCCC TCACCAGACA GAATATCTCA 
ATGGAAGGGT CCCCAAGCAA GACCCCCTCT GTCTCAACAT CTGATAATCT GCCCAATGCT 
GGAAGGCCCC TGGGAAACCC CTTTTCCAGG CAGAGTTCTC TCTGTAGCAC CGGAAGTTCT 
AGCAGCCAGC ACGGAAGCCC AAGCAAGTTA CCCTTGAGGA TGCTCACAAG GCCGGAAGGA 
CTCCTCACTC CTGGAGTGGA AGACCAACGG CTCTCTACTC AGGAAGAGTG TCCTGAAAGA 
GGTGTACATG AGCAATCTAG CAGTGACCAC CATAGGCATC CATCTCCTCT TTCAGACTGC 
CCTCAGTTCC TGCACGTTCC AGGGAGGATG CAGCGCCCAA GCAATTTGGA AACAAGTGGG 
ATATCTAAGC TAGAAACTTC TGGAAGGCAT CCAGATGCTT CTTCAACTGG GACTAGTGTC 
GTGAGCCCAG AAGCCCCCCT CTCACCCTCC ATCGAAGAAA AGGTCATGTT GTGCATTCAG 
GAGAATGTGG AAAAGGGCCA AGTGCAGACA AAGTCTGCCT CTGTGGAAGT GAAGCCCAGG 
GCAGGGCCTT CGTTCACCAG CTGGTTTGGT TTTCGAAAGA GCAGGCTTCC AGCTCTCAGT 
AGCAGGAGAA TGGAAGCTTC CAAGAGTAAA GTGGAAAAGA AAGACACAAA ACTGAAATCT 
GAGAGGAAAA AAGAAAAGAA GAAAGCTGAA GTACAATCAA AGATAGAAAA CGAACTGAGT 
AGGGGGACCA AGAAGGCAGA TGGTCAGAGT CCAGACGATG GTCTGCAAAG CTCAGAGAGT 
CTGAAAGTAT CCCAAGACAT TTATAACCAA ATGAAGTTGG AGCCAAGGAA TAGACCCAGC 
CCTGCTGTGT GCCCAACAAA AGATGCCTTC ATGACAGAAC TTCTAAACAG AGTTGATAAG 
AGAGCCTCGG TACAGAGAGA GAGTGGATCA AAGGGTGTTT CCTGCAGGAG CGTGTTAGAG 
GGCACCTCCC AGGGCTCCTG TTTCGCCAGC GGCTCTGTGA GCACTCAAGG AAGTCAAAAG 
AAAAACATCA AAACCAAAGT CGATATGGAA AAACCAAGAG AATCCTTGGG GGCCGAGGTC 
AATGAAGATG TACAAGAAGA TGAAGAAGAT AGAGTTGCTG ACACTACTCT CCAGAGCCAC 
ATGATAGAAT CCAACTGCCA GATGAGAACA CTGGACAGCG GGATTGGAAC CTTTCCCCTC 
CCAGACTCAG GAAACCGCTC AGTGGGACGA TACATATGCC AGCAGGACTC TCCAGAGGAC 
ACTGATGCCC TCCTGCCTCT CCAGCCAGCC GCTTCCATGG CATCCTCTGC CAGAGCCCAA 
ACCCTTGACC GCGAAGTGCC TTCCTCCACA GACAGCCAGA GCTCCCCGGA GTCCATGATC 
ATCCATTCCA TATCTGACCC CAGCATGATG GCCAGAGAGA TGAGAGCCTC ACAGAGTCAC 
CTTCCCAAGC CTACCTCCTC AGGAAAACCT AGTTTTGAAA AGCAAAATGG AGCAGAGTCA 
AAGGCTCACA CTGGCCCATC ATTCGAATAT GCTGAGGATG CAGTGGCAAG TGAGCCCCTT 
TCAGGATGGA GGGGTGAAGA TGCTACTACT GAGACCCAGA ACAGGTGCCC TGAGGAAGCA 
GAAATCCTTC ACAGACTTGA AAGTGACTCG AGGACAGTTA ATCGTCACCG TGCTCTAGTG 
AGGCAGGAAC CGAAACAAGT TGAAGAAACA AAAGAAGATC CTGAGAATAG ATTATCTGAA 
ATTTCCCTGG AGTCATTCAA TAAATTTAGC AGCAATACTG TGATTTTATT AGGAAAAGAG 
AAGTCTCCAA GTGAGGTTGA AGGACAAAAG GAAGAGAAAG GCAAAAAGAC AGAAGTGTCT 
TCGAGTAGCT CCGAACGGCC TGGGGTGGAC CGCGTGGAGT CTCTGAGTGA CTCTCTGTAT 
GACAGTTTCT CCTCTTGTGC AAGTCAAGCA TCCAACGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529394.1
CDS	2651..8275
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_006529331.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529331.4

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG CAATCATCGC GGATCTGGGA TAAAAGACTA 
AGTATTGATT CTTCGCTCCC AAGTGGCTTC GCCAGCCCTG CAGATGAACT ACCTCCGACC 
CGTATCAAAG AAAGCCACAT TCTAGAGGGA CTGAGGAAGC TACAGAAGCG GAAAGTGTTC 
CTTGAATCAC CATCATTGGT GATAAGCAAA TGGGGTTATA AAGATTGCAT GAACTCGAAT 
GAAGGGATCT ATTCGCCAGG AATTAAAACC AGCAGCCTCA AGGAGTGTCC GCCCTGCCAA 
CCGTCTGGCA TGGGGATCCC CTGCCAGAAT CTCCATCAGA CATTCATCTA TGATGCAAAT 
TCTCATGAGG ATGCACATGA AGAACTCCCA TCCTCAGCCT TGCCACTAAC ATCTCCGAGC 
CAGGGCTACA GAATACAGGG CAGTAAATTG ACACACAGTG TTTCAGACAG TCTGTTTAAC 
TGGGAGCCAC ATAGGAAGTA CTTTGCAGAA GGTACAGCCT CAGTATATAC CAGAGAAATG 
CCTGAAAGGC TAGCTCGTTG TGCTAGCCAC TGCCCCTTGG AGAGGAAGCT ATGTCCCAGT 
ATACACCTGC CCTGGGTGCA AAGAGACAGG GACCCACACA GCCAAGTTCA TGGCCATGTG 
CCTCTGAACC TGCAGCTCTC AGATACCGAT GACAATGAGA CCCTAAACGA GCTACACATA 
GAGAGCAGTG ATGAGAGAAG CCCTTCAGAC CTGTCCTTGA CTGGGGACAC CGACAAGTCC 
ATGGAGAACC TAGAGGTGGG ATTCAGAAAG TCTGAAGGTG GCTCTCGGGA CATGGGAGAA 
GAAGCATGTC CCACCCACTT AGAGACTAGC CCGAAGGCAT TCAGTTTCAT CAAGCAACAA 
AGGGTTGTCA AGAGGACCTC TTCGGAAGAA TGTGTCACGG TCATATTTGA TGCAGAAAAT 
GGAGAGCCAG TGGAATTCAG CTCTCACCAG GCTGGAGTCG TCACTGTCAC CAGGGATGAA 
ATTTCCATCA GCTCAGCCCT TGCTGGGCCC TCAGAGGAAC TCACTGAACT TTCACCTCAG 
GAAATTACTC ACTTCCAGGC AGGAGCTACT GCAAGAGACT GTCCTTTTCT GAAGAGACCT 
GAAGAGGAGA CTGCGAAAAA CATTCCAAAA GATGAAAATC ATATTCTCAC AGCTCCGAAT 
GACTTCTTCA GCTCCCAGAC TATAATAACT CAGAATTCCC CAAGACCAAA GCTCGCCAAA 
CCCACACAGG GGGTAGCCTG GCAGAGTAAT TCTAGACCTG TTGGAAACAT GGGTGTCTAC 
ACAAAGCAAG GTCTGACAAA AATCCCTTCC AGAGGCAAGT CTTCACCTCA GAAATCCAAA 
CTTGTGGAGT CTATGCCCAC CATGCTGGTC CCCTCCAGTG CCCTAGTGAC TCCTGAAAAG 
ACACCAGCAT CCCCTATGGG GAAACTCTCA CAATGTAGAA AGGCAGAGAG CCCTGGTTCT 
CTCTGTGATG TACAGCCAGA ATCACACATT CCAAAACACC CTGCCCAGGT TCCTCATATC 
CCCAAAATTT CCAATAAGAA GAACTGGGTT CAGTGCCCCA GGAGTCAGAC TTCAGGGATC 
CAGTCACGGC ATCTCATGGG GAGAAGTGAC AGTAGTGAAA TGAGGTCCAA TGGCTGTGTC 
TCTGCGCCAC AGACAAGAAT GAGGTCTCCC TCCCCTCCAA CCCCTCCAGG GAGATCAGTG 
TCACTCCTGG TTAGACCCAG TTATGATTAC CCACCACTAC CATCACCTGC CAAACCTGAA 
ACTCAGGGCC CTGGAGACGC TGTAAGTCCA GGGTTGAAAT CCCCGCTGTT GAAAGGATCC 
TCTGCTCCTC TTGTCTCTTC CAGTCCAGTG ATGTCAGAAA TACAGAAGAA ACCATCTGTG 
GCCTCCAGAA AGTCTGCCTT CCCTCCAGCT CTTCCCTCAA CCCAAGCGGT AGTGCACACC 
AGATACCCTG AACACATAGC CTCCAGTCCA TTTGCTGTGA CGACTTCAGG GCCCCCAAAA 
GTCTCTCCAA AAAGAGGCAT CCCTAAACCT CCCCCTCACC ATGCACTAGG AACTGCCCAC 
ATGGACACAG ACTTACAGAC ACCCAAGACT TGTCCTTCAA GCTGTGAACT GCTAGAGGTG 
ACATCTTGTA AAAGCCTGTC TCCAGGGAGA AAAAGACAGT TGAGTGACAG CACTGTGATG 
CCCCACAGGC CTTCCTTCTC AGGAGTGAAT GAGTCCTTGA CACCTCAGGT TAACAGTCCA 
TCCTCCTCTT CCTCATCCTG TAGAAACCAA GGGTCCTCAC AAGGTGGCCA GAACTCTCCC 
GAGAAAGGAC CTAAAACTCG CCTCCCAGTA GGACTCAAAG TTCTCATGAA GTCTCCTCAG 
TTGCTCAAGA AAACTTCCTT GGTACCAGGG AAACAAGAAA AGGATAGTTT AAATGAAGCA 
TCTCGAAGCT GTGTAGCAGC AAACACGTCT AATCCTGAGG ACTCCAAAGA CCCTGCAAGT 
CTGGAGACCT CAAGAGATGA GAGAACCACA GCCTTACTAG GTGTGAGAGG TCCCGACAGT 
CTAGCTAGAG GACTTGCCTT AGACTCAACA ATGCCAGAAT CGTTAGAATG TAGCATCTCC 
AGAGATGATG GAAGAGATGG GGCAGACAGC AGAGCCATAA AAAGATCCTT CTCCTCCAGC 
AAGCCACACC TAAAGCCTGC TCTAGGCATG AATGGAGCCA AAGCTCGGAG TCAGAGCTTC 
AGCATCCACT TGGGAGAGAA GCCTTCCACA CCCCCAACAG AAGGGCCAGG CAAGATTCGA 
ACTCAGATTA TCACCAACAC GGCTGAAAGA GGCAACTCCC TCACCAGACA GAATATCTCA 
ATGGAAGGGT CCCCAAGCAA GACCCCCTCT GTCTCAACAT CTGATAATCT GCCCAATGCT 
GGAAGGCCCC TGGGAAACCC CTTTTCCAGG CAGAGTTCTC TCTGTAGCAC CGGAAGTTCT 
AGCAGCCAGC ACGGAAGCCC AAGCAAGTTA CCCTTGAGGA TGCTCACAAG GCCGGAAGGA 
CTCCTCACTC CTGGAGTGGA AGACCAACGG CTCTCTACTC AGGAAGAGTG TCCTGAAAGA 
GGTGTACATG AGCAATCTAG CAGTGACCAC CATAGGCATC CATCTCCTCT TTCAGACTGC 
CCTCAGTTCC TGCACGTTCC AGGGAGGATG CAGCGCCCAA GCAATTTGGA AACAAGTGGG 
ATATCTAAGC TAGAAACTTC TGGAAGGCAT CCAGATGCTT CTTCAACTGG GACTAGTGTC 
GTGAGCCCAG AAGCCCCCCT CTCACCCTCC ATCGAAGAAA AGGTCATGTT GTGCATTCAG 
GAGAATGTGG AAAAGGGCCA AGTGCAGACA AAGTCTGCCT CTGTGGAAGT GAAGCCCAGG 
GCAGGGCCTT CGTTCACCAG CTGGTTTGGT TTTCGAAAGA GCAGGCTTCC AGCTCTCAGT 
AGCAGGAGAA TGGAAGCTTC CAAGAGTAAA GTGGAAAAGA AAGACACAAA ACTGAAATCT 
GAGAGGAAAA AAGAAAAGAA GAAAGCTGAA GTACAATCAA AGATAGAAAA CGAACTGAGT 
AGGGGGACCA AGAAGGCAGA TGGTCAGAGT CCAGACGATG GTCTGCAAAG CTCAGAGAGT 
CTGAAAGTAT CCCAAGACAT TTATAACCAA ATGAAGTTGG AGCCAAGGAA TAGACCCAGC 
CCTGCTGTGT GCCCAACAAA AGATGCCTTC ATGACAGAAC TTCTAAACAG AGTTGATAAG 
AGAGCCTCGG TACAGAGAGA GAGTGGATCA AAGGGTGTTT CCTGCAGGAG CGTGTTAGAG 
GGCACCTCCC AGGGCTCCTG TTTCGCCAGC GGCTCTGTGA GCACTCAAGG AAGTCAAAAG 
AAAAACATCA AAACCAAAGT CGATATGGAA AAACCAAGAG AATCCTTGGG GGCCGAGGTC 
AATGAAGATG TACAAGAAGA TGAAGAAGAT AGAGTTGCTG ACACTACTCT CCAGAGCCAC 
ATGATAGAAT CCAACTGCCA GATGAGAACA CTGGACAGCG GGATTGGAAC CTTTCCCCTC 
CCAGACTCAG GAAACCGCTC AGTGGGACGA TACATATGCC AGCAGGACTC TCCAGAGGAC 
ACTGATGCCC TCCTGCCTCT CCAGCCAGCC GCTTCCATGG CATCCTCTGC CAGAGCCCAA 
ACCCTTGACC GCGAAGTGCC TTCCTCCACA GACAGCCAGA GCTCCCCGGA GTCCATGATC 
ATCCATTCCA TATCTGACCC CAGCATGATG GCCAGAGAGA TGAGAGCCTC ACAGAGTCAC 
CTTCCCAAGC CTACCTCCTC AGGAAAACCT AGTTTTGAAA AGCAAAATGG AGCAGAGTCA 
AAGGCTCACA CTGGCCCATC ATTCGAATAT GCTGAGGATG CAGTGGCAAG TGAGCCCCTT 
TCAGGATGGA GGGGTGAAGA TGCTACTACT GAGACCCAGA ACAGGTGCCC TGAGGAAGCA 
GAAATCCTTC ACAGACTTGA AAGTGACTCG AGGACAGTTA ATCGTCACCG TGCTCTAGTG 
AGGCAGGAAC CGAAACAAGT TGAAGAAACA AAAGAAGATC CTGAGAATAG ATTATCTGAA 
ATTTCCCTGG AGTCATTCAA TAAATTTAGC AGCAATACTG TGATTTTATT AGGAAAAGAG 
AAGTCTCCAA GTGAGGTTGA AGGACAAAAG GAAGAGAAAG GCAAAAAGAC AGAAGTGTCT 
TCGAGTAGCT CCGAACGGCC TGGGGTGGAC CGCGTGGAGT CTCTGAGTGA CTCTCTGTAT 
GACAGTTTCT CCTCTTGTGC AAGTCAAGCA TCCAACGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_030252628.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108488.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X11, mRNA.

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CloneID	OMu28059
Clone ID Related Accession (Same CDS sequence)	XM_017319542.1 , XM_006529332.3 , XM_030252628.1
Accession Version	XM_030252628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGAGA AGCTGATACA TGAGTTGGAA GAGGAACGGC ACTTGCGGCT ACAGAGTGAG 
GAGCGGCTGC GGGAGGTGAC CCTGGAGTCA GAGCGCAACA GGATCCAGAT GCTCGGCCTG 
CAGCAGCAGT TTTCCAGAAT GGAAGAAACT GTCCGAAGTC TGTTGCAGAG CCAAGGATCT 
CCAGAGCAGA AAAAGGAAGA ACCTGCCAAG ATCACAGCCT ACCAGGAAGG ACTACCTGAT 
GAAGAGAGAA AAGAGCAAGC AGCACTGGGT GATCTCCATG TGGTGGCAGC TGATGAGGAC 
TCAAGAAGCG AGCACAGTAG TGTGGAGGAG GGAAAGGAAG ACAGCAGACT GCTCCTGAAG 
AGACTGAAGG CCCTGGAGGC GGAGAATTCA GCACTGGCAC TGGAAAACGA GAATCAGAGA 
GAACAGTATG AGCGATGTCT TGATGAGGTA GCCAACCAAG TTGTTCGAGC TCTACTCACT 
CAAAAGGATC TGAGGGAGGA GTGTGTAAAG TTGAAAACAA GAGTGTTTGA CTTGGAGCAA 
CAGAACCGAG CACTCAGCCT CCTGTTCCAA CAGCGAGTCA GACCAGCTTC TGATGTGCTT 
CTCCAGGAAC ACCTCCAGAA TGCAAAATCA GGAACCCCTG CCTTGAAACA CTCTGGCTCA 
GGGGTTGTCA TCCCTGATCA CCTCTGTCCT CGCAACAGCT GCAGCAGTGG CAGTGAGCTA 
TCGCTGTCAA GCACCTGCAG CGAGTACTCC AGTGGCTCCT CCTACACGTG GCAAGACAGG 
AAGCTCATTG GGAAAAGGCA ATCATCGCGG ATCTGGGATA AAAGACTAAG TATTGATTCT 
TCGCTCCCAA GTGGCTTCGC CAGCCCTGCA GATGAACTAC CTCCGACCCG TATCAAAGAA 
AGCCACATTC TAGAGGGACT GAGGAAGCTA CAGAAGCGGA AAGTGTTCCT TGAATCACCA 
TCATTGGTGA TAAGCAAATG GGGTTATAAA GATTGCATGA ACTCGAATGA AGGGATCTAT 
TCGCCAGGAA TTAAAACCAG CAGCCTCAAG GAGTGTCCGC CCTGCCAACC GTCTGGCATG 
GGGATCCCCT GCCAGAATCT CCATCAGACA TTCATCTATG ATGCAAATTC TCATGAGGAT 
GCACATGAAG AACTCCCATC CTCAGCCTTG CCACTAACAT CTCCGAGCCA GGGCTACAGA 
ATACAGGGCA GTAAATTGAC ACACAGTGTT TCAGACAGTC TGTTTAACTG GGAGCCACAT 
AGGAAGTACT TTGCAGAAGG TACAGCCTCA GTATATACCA GAGAAATGCC TGAAAGGCTA 
GCTCGTTGTG CTAGCCACTG CCCCTTGGAG AGGAAGCTAT GTCCCAGTAT ACACCTGCCC 
TGGGTGCAAA GAGACAGGGA CCCACACAGC CAAGTTCATG GCCATGTGCC TCTGAACCTG 
CAGCTCTCAG ATACCGATGA CAATGAGACC CTAAACGAGC TACACATAGA GAGCAGTGAT 
GAGAGAAGCC CTTCAGACCT GTCCTTGACT GGGGACACCG ACAAGTCCAT GGAGAACCTA 
GAGGTGGGAT TCAGAAAGTC TGAAGGTGGC TCTCGGGACA TGGGAGAAGA AGCATGTCCC 
ACCCACTTAG AGACTAGCCC GAAGGCATTC AGTTTCATCA AGCAACAAAG GGTTGTCAAG 
AGGACCTCTT CGGAAGAATG TGTCACGGTC ATATTTGATG CAGAAAATGG AGAGCCAGTG 
GAATTCAGCT CTCACCAGGC TGGAGTCGTC ACTGTCACCA GGGATGAAAT TTCCATCAGC 
TCAGCCCTTG CTGGGCCCTC AGAGGAACTC ACTGAACTTT CACCTCAGGA AATTACTCAC 
TTCCAGGCAG GAGCTACTGC AAGAGACTGT CCTTTTCTGA AGAGACCTGA AGAGGAGACT 
GCGAAAAACA TTCCAAAAGA TGAAAATCAT ATTCTCACAG CTCCGAATGA CTTCTTCAGC 
TCCCAGACTA TAATAACTCA GAATTCCCCA AGACCAAAGC TCGCCAAACC CACACAGGGG 
GTAGCCTGGC AGAGTAATTC TAGACCTGTT GGAAACATGG GTGTCTACAC AAAGCAAGGT 
CTGACAAAAA TCCCTTCCAG AGGCAAGTCT TCACCTCAGA AATCCAAACT TGTGGAGTCT 
ATGCCCACCA TGCTGGTCCC CTCCAGTGCC CTAGTGACTC CTGAAAAGAC ACCAGCATCC 
CCTATGGGGA AACTCTCACA ATGTAGAAAG GCAGAGAGCC CTGGTTCTCT CTGTGATGTA 
CAGCCAGAAT CACACATTCC AAAACACCCT GCCCAGGTTC CTCATATCCC CAAAATTTCC 
AATAAGAAGA ACTGGGTTCA GTGCCCCAGG AGTCAGACTT CAGGGATCCA GTCACGGCAT 
CTCATGGGGA GAAGTGACAG TAGTGAAATG AGGTCCAATG GCTGTGTCTC TGCGCCACAG 
ACAAGAATGA GGTCTCCCTC CCCTCCAACC CCTCCAGGGA GATCAGTGTC ACTCCTGGTT 
AGACCCAGTT ATGATTACCC ACCACTACCA TCACCTGCCA AACCTGAAAC TCAGGGCCCT 
GGAGACGCTG TAAGTCCAGG GTTGAAATCC CCGCTGTTGA AAGGATCCTC TGCTCCTCTT 
GTCTCTTCCA GTCCAGTGAT GTCAGAAATA CAGAAGAAAC CATCTGTGGC CTCCAGAAAG 
TCTGCCTTCC CTCCAGCTCT TCCCTCAACC CAAGCGGTAG TGCACACCAG ATACCCTGAA 
CACATAGCCT CCAGTCCATT TGCTGTGACG ACTTCAGGGC CCCCAAAAGT CTCTCCAAAA 
AGAGGCATCC CTAAACCTCC CCCTCACCAT GCACTAGGAA CTGCCCACAT GGACACAGAC 
TTACAGACAC CCAAGACTTG TCCTTCAAGC TGTGAACTGC TAGAGGTGAC ATCTTGTAAA 
AGCCTGTCTC CAGGGAGAAA AAGACAGTTG AGTGACAGCA CTGTGATGCC CCACAGGCCT 
TCCTTCTCAG GAGTGAATGA GTCCTTGACA CCTCAGGTTA ACAGTCCATC CTCCTCTTCC 
TCATCCTGTA GAAACCAAGG GTCCTCACAA GGTGGCCAGA ACTCTCCCGA GAAAGGACCT 
AAAACTCGCC TCCCAGTAGG ACTCAAAGTT CTCATGAAGT CTCCTCAGTT GCTCAAGAAA 
ACTTCCTTGG TACCAGGGAA ACAAGAAAAG GATAGTTTAA ATGAAGCATC TCGAAGCTGT 
GTAGCAGCAA ACACGTCTAA TCCTGAGGAC TCCAAAGACC CTGCAAGTCT GGAGACCTCA 
AGAGATGAGA GAACCACAGC CTTACTAGGT GTGAGAGGTC CCGACAGTCT AGCTAGAGGA 
CTTGCCTTAG ACTCAACAAT GCCAGAATCG TTAGAATGTA GCATCTCCAG AGATGATGGA 
AGAGATGGGG CAGACAGCAG AGCCATAAAA AGATCCTTCT CCTCCAGCAA GCCACACCTA 
AAGCCTGCTC TAGGCATGAA TGGAGCCAAA GCTCGGAGTC AGAGCTTCAG CATCCACTTG 
GGAGAGAAGC CTTCCACACC CCCAACAGAA GGGCCAGGCA AGATTCGAAC TCAGATTATC 
ACCAACACGG CTGAAAGAGG CAACTCCCTC ACCAGACAGA ATATCTCAAT GGAAGGGTCC 
CCAAGCAAGA CCCCCTCTGT CTCAACATCT GATAATCTGC CCAATGCTGG AAGGCCCCTG 
GGAAACCCCT TTTCCAGGCA GAGTTCTCTC TGTAGCACCG GAAGTTCTAG CAGCCAGCAC 
GGAAGCCCAA GCAAGTTACC CTTGAGGATG CTCACAAGGC CGGAAGGACT CCTCACTCCT 
GGAGTGGAAG ACCAACGGCT CTCTACTCAG GAAGAGTGTC CTGAAAGAGG TGTACATGAG 
CAATCTAGCA GTGACCACCA TAGGCATCCA TCTCCTCTTT CAGACTGCCC TCAGTTCCTG 
CACGTTCCAG GGAGGATGCA GCGCCCAAGC AATTTGGAAA CAAGTGGGAT ATCTAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCTTCT TCAACTGGGA CTAGTGTCGT GAGCCCAGAA 
GCCCCCCTCT CACCCTCCAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA GAATGTGGAA 
AAGGGCCAAG TGCAGACAAA GTCTGCCTCT GTGGAAGTGA AGCCCAGGGC AGGGCCTTCG 
TTCACCAGCT GGTTTGGTTT TCGAAAGAGC AGGCTTCCAG CTCTCAGTAG CAGGAGAATG 
GAAGCTTCCA AGAGTAAAGT GGAAAAGAAA GACACAAAAC TGAAATCTGA GAGGAAAAAA 
GAAAAGAAGA AAGCTGAAGT ACAATCAAAG ATAGAAAACG AACTGAGTAG GGGGACCAAG 
AAGGCAGATG GTCAGAGTCC AGACGATGGT CTGCAAAGCT CAGAGAGTCT GAAAGTATCC 
CAAGACATTT ATAACCAAAT GAAGTTGGAG CCAAGGAATA GACCCAGCCC TGCTGTGTGC 
CCAACAAAAG ATGCCTTCAT GACAGAACTT CTAAACAGAG TTGATAAGAG AGCCTCGGTA 
CAGAGAGAGA GTGGATCAAA GGGTGTTTCC TGCAGGAGCG TGTTAGAGGG CACCTCCCAG 
GGCTCCTGTT TCGCCAGCGG CTCTGTGAGC ACTCAAGGAA GTCAAAAGAA AAACATCAAA 
ACCAAAGTCG ATATGGAAAA ACCAAGAGAA TCCTTGGGGG CCGAGGTCAA TGAAGATGTA 
CAAGAAGATG AAGAAGATAG AGTTGCTGAC ACTACTCTCC AGAGCCACAT GATAGAATCC 
AACTGCCAGA TGAGAACACT GGACAGCGGG ATTGGAACCT TTCCCCTCCC AGACTCAGGA 
AACCGCTCAG TGGGACGATA CATATGCCAG CAGGACTCTC CAGAGGACAC TGATGCCCTC 
CTGCCTCTCC AGCCAGCCGC TTCCATGGCA TCCTCTGCCA GAGCCCAAAC CCTTGACCGC 
GAAGTGCCTT CCTCCACAGA CAGCCAGAGC TCCCCGGAGT CCATGATCAT CCATTCCATA 
TCTGACCCCA GCATGATGGC CAGAGAGATG AGAGCCTCAC AGAGTCACCT TCCCAAGCCT 
ACCTCCTCAG GAAAACCTAG TTTTGAAAAG CAAAATGGAG CAGAGTCAAA GGCTCACACT 
GGCCCATCAT TCGAATATGC TGAGGATGCA GTGGCAAGTG AGCCCCTTTC AGGATGGAGG 
GGTGAAGATG CTACTACTGA GACCCAGAAC AGGTGCCCTG AGGAAGCAGA AATCCTTCAC 
AGACTTGAAA GTGACTCGAG GACAGTTAAT CGTCACCGTG CTCTAGTGAG GCAGGAACCG 
AAACAAGTTG AAGAAACAAA AGAAGATCCT GAGAATAGAT TATCTGAAAT TTCCCTGGAG 
TCATTCAATA AATTTAGCAG CAATACTGTG ATTTTATTAG GAAAAGAGAA GTCTCCAAGT 
GAGGTTGAAG GACAAAAGGA AGAGAAAGGC AAAAAGACAG AAGTGTCTTC GAGTAGCTCC 
GAACGGCCTG GGGTGGACCG CGTGGAGTCT CTGAGTGACT CTCTGTATGA CAGTTTCTCC 
TCTTGTGCAA GTCAAGCATC CAACGAGGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108488.1
CDS	102..5714
Translation	MHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_030252628.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252628.1

ORF Insert Sequence:

ATGCACGAGA AGCTGATACA TGAGTTGGAA GAGGAACGGC ACTTGCGGCT ACAGAGTGAG 
GAGCGGCTGC GGGAGGTGAC CCTGGAGTCA GAGCGCAACA GGATCCAGAT GCTCGGCCTG 
CAGCAGCAGT TTTCCAGAAT GGAAGAAACT GTCCGAAGTC TGTTGCAGAG CCAAGGATCT 
CCAGAGCAGA AAAAGGAAGA ACCTGCCAAG ATCACAGCCT ACCAGGAAGG ACTACCTGAT 
GAAGAGAGAA AAGAGCAAGC AGCACTGGGT GATCTCCATG TGGTGGCAGC TGATGAGGAC 
TCAAGAAGCG AGCACAGTAG TGTGGAGGAG GGAAAGGAAG ACAGCAGACT GCTCCTGAAG 
AGACTGAAGG CCCTGGAGGC GGAGAATTCA GCACTGGCAC TGGAAAACGA GAATCAGAGA 
GAACAGTATG AGCGATGTCT TGATGAGGTA GCCAACCAAG TTGTTCGAGC TCTACTCACT 
CAAAAGGATC TGAGGGAGGA GTGTGTAAAG TTGAAAACAA GAGTGTTTGA CTTGGAGCAA 
CAGAACCGAG CACTCAGCCT CCTGTTCCAA CAGCGAGTCA GACCAGCTTC TGATGTGCTT 
CTCCAGGAAC ACCTCCAGAA TGCAAAATCA GGAACCCCTG CCTTGAAACA CTCTGGCTCA 
GGGGTTGTCA TCCCTGATCA CCTCTGTCCT CGCAACAGCT GCAGCAGTGG CAGTGAGCTA 
TCGCTGTCAA GCACCTGCAG CGAGTACTCC AGTGGCTCCT CCTACACGTG GCAAGACAGG 
AAGCTCATTG GGAAAAGGCA ATCATCGCGG ATCTGGGATA AAAGACTAAG TATTGATTCT 
TCGCTCCCAA GTGGCTTCGC CAGCCCTGCA GATGAACTAC CTCCGACCCG TATCAAAGAA 
AGCCACATTC TAGAGGGACT GAGGAAGCTA CAGAAGCGGA AAGTGTTCCT TGAATCACCA 
TCATTGGTGA TAAGCAAATG GGGTTATAAA GATTGCATGA ACTCGAATGA AGGGATCTAT 
TCGCCAGGAA TTAAAACCAG CAGCCTCAAG GAGTGTCCGC CCTGCCAACC GTCTGGCATG 
GGGATCCCCT GCCAGAATCT CCATCAGACA TTCATCTATG ATGCAAATTC TCATGAGGAT 
GCACATGAAG AACTCCCATC CTCAGCCTTG CCACTAACAT CTCCGAGCCA GGGCTACAGA 
ATACAGGGCA GTAAATTGAC ACACAGTGTT TCAGACAGTC TGTTTAACTG GGAGCCACAT 
AGGAAGTACT TTGCAGAAGG TACAGCCTCA GTATATACCA GAGAAATGCC TGAAAGGCTA 
GCTCGTTGTG CTAGCCACTG CCCCTTGGAG AGGAAGCTAT GTCCCAGTAT ACACCTGCCC 
TGGGTGCAAA GAGACAGGGA CCCACACAGC CAAGTTCATG GCCATGTGCC TCTGAACCTG 
CAGCTCTCAG ATACCGATGA CAATGAGACC CTAAACGAGC TACACATAGA GAGCAGTGAT 
GAGAGAAGCC CTTCAGACCT GTCCTTGACT GGGGACACCG ACAAGTCCAT GGAGAACCTA 
GAGGTGGGAT TCAGAAAGTC TGAAGGTGGC TCTCGGGACA TGGGAGAAGA AGCATGTCCC 
ACCCACTTAG AGACTAGCCC GAAGGCATTC AGTTTCATCA AGCAACAAAG GGTTGTCAAG 
AGGACCTCTT CGGAAGAATG TGTCACGGTC ATATTTGATG CAGAAAATGG AGAGCCAGTG 
GAATTCAGCT CTCACCAGGC TGGAGTCGTC ACTGTCACCA GGGATGAAAT TTCCATCAGC 
TCAGCCCTTG CTGGGCCCTC AGAGGAACTC ACTGAACTTT CACCTCAGGA AATTACTCAC 
TTCCAGGCAG GAGCTACTGC AAGAGACTGT CCTTTTCTGA AGAGACCTGA AGAGGAGACT 
GCGAAAAACA TTCCAAAAGA TGAAAATCAT ATTCTCACAG CTCCGAATGA CTTCTTCAGC 
TCCCAGACTA TAATAACTCA GAATTCCCCA AGACCAAAGC TCGCCAAACC CACACAGGGG 
GTAGCCTGGC AGAGTAATTC TAGACCTGTT GGAAACATGG GTGTCTACAC AAAGCAAGGT 
CTGACAAAAA TCCCTTCCAG AGGCAAGTCT TCACCTCAGA AATCCAAACT TGTGGAGTCT 
ATGCCCACCA TGCTGGTCCC CTCCAGTGCC CTAGTGACTC CTGAAAAGAC ACCAGCATCC 
CCTATGGGGA AACTCTCACA ATGTAGAAAG GCAGAGAGCC CTGGTTCTCT CTGTGATGTA 
CAGCCAGAAT CACACATTCC AAAACACCCT GCCCAGGTTC CTCATATCCC CAAAATTTCC 
AATAAGAAGA ACTGGGTTCA GTGCCCCAGG AGTCAGACTT CAGGGATCCA GTCACGGCAT 
CTCATGGGGA GAAGTGACAG TAGTGAAATG AGGTCCAATG GCTGTGTCTC TGCGCCACAG 
ACAAGAATGA GGTCTCCCTC CCCTCCAACC CCTCCAGGGA GATCAGTGTC ACTCCTGGTT 
AGACCCAGTT ATGATTACCC ACCACTACCA TCACCTGCCA AACCTGAAAC TCAGGGCCCT 
GGAGACGCTG TAAGTCCAGG GTTGAAATCC CCGCTGTTGA AAGGATCCTC TGCTCCTCTT 
GTCTCTTCCA GTCCAGTGAT GTCAGAAATA CAGAAGAAAC CATCTGTGGC CTCCAGAAAG 
TCTGCCTTCC CTCCAGCTCT TCCCTCAACC CAAGCGGTAG TGCACACCAG ATACCCTGAA 
CACATAGCCT CCAGTCCATT TGCTGTGACG ACTTCAGGGC CCCCAAAAGT CTCTCCAAAA 
AGAGGCATCC CTAAACCTCC CCCTCACCAT GCACTAGGAA CTGCCCACAT GGACACAGAC 
TTACAGACAC CCAAGACTTG TCCTTCAAGC TGTGAACTGC TAGAGGTGAC ATCTTGTAAA 
AGCCTGTCTC CAGGGAGAAA AAGACAGTTG AGTGACAGCA CTGTGATGCC CCACAGGCCT 
TCCTTCTCAG GAGTGAATGA GTCCTTGACA CCTCAGGTTA ACAGTCCATC CTCCTCTTCC 
TCATCCTGTA GAAACCAAGG GTCCTCACAA GGTGGCCAGA ACTCTCCCGA GAAAGGACCT 
AAAACTCGCC TCCCAGTAGG ACTCAAAGTT CTCATGAAGT CTCCTCAGTT GCTCAAGAAA 
ACTTCCTTGG TACCAGGGAA ACAAGAAAAG GATAGTTTAA ATGAAGCATC TCGAAGCTGT 
GTAGCAGCAA ACACGTCTAA TCCTGAGGAC TCCAAAGACC CTGCAAGTCT GGAGACCTCA 
AGAGATGAGA GAACCACAGC CTTACTAGGT GTGAGAGGTC CCGACAGTCT AGCTAGAGGA 
CTTGCCTTAG ACTCAACAAT GCCAGAATCG TTAGAATGTA GCATCTCCAG AGATGATGGA 
AGAGATGGGG CAGACAGCAG AGCCATAAAA AGATCCTTCT CCTCCAGCAA GCCACACCTA 
AAGCCTGCTC TAGGCATGAA TGGAGCCAAA GCTCGGAGTC AGAGCTTCAG CATCCACTTG 
GGAGAGAAGC CTTCCACACC CCCAACAGAA GGGCCAGGCA AGATTCGAAC TCAGATTATC 
ACCAACACGG CTGAAAGAGG CAACTCCCTC ACCAGACAGA ATATCTCAAT GGAAGGGTCC 
CCAAGCAAGA CCCCCTCTGT CTCAACATCT GATAATCTGC CCAATGCTGG AAGGCCCCTG 
GGAAACCCCT TTTCCAGGCA GAGTTCTCTC TGTAGCACCG GAAGTTCTAG CAGCCAGCAC 
GGAAGCCCAA GCAAGTTACC CTTGAGGATG CTCACAAGGC CGGAAGGACT CCTCACTCCT 
GGAGTGGAAG ACCAACGGCT CTCTACTCAG GAAGAGTGTC CTGAAAGAGG TGTACATGAG 
CAATCTAGCA GTGACCACCA TAGGCATCCA TCTCCTCTTT CAGACTGCCC TCAGTTCCTG 
CACGTTCCAG GGAGGATGCA GCGCCCAAGC AATTTGGAAA CAAGTGGGAT ATCTAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCTTCT TCAACTGGGA CTAGTGTCGT GAGCCCAGAA 
GCCCCCCTCT CACCCTCCAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA GAATGTGGAA 
AAGGGCCAAG TGCAGACAAA GTCTGCCTCT GTGGAAGTGA AGCCCAGGGC AGGGCCTTCG 
TTCACCAGCT GGTTTGGTTT TCGAAAGAGC AGGCTTCCAG CTCTCAGTAG CAGGAGAATG 
GAAGCTTCCA AGAGTAAAGT GGAAAAGAAA GACACAAAAC TGAAATCTGA GAGGAAAAAA 
GAAAAGAAGA AAGCTGAAGT ACAATCAAAG ATAGAAAACG AACTGAGTAG GGGGACCAAG 
AAGGCAGATG GTCAGAGTCC AGACGATGGT CTGCAAAGCT CAGAGAGTCT GAAAGTATCC 
CAAGACATTT ATAACCAAAT GAAGTTGGAG CCAAGGAATA GACCCAGCCC TGCTGTGTGC 
CCAACAAAAG ATGCCTTCAT GACAGAACTT CTAAACAGAG TTGATAAGAG AGCCTCGGTA 
CAGAGAGAGA GTGGATCAAA GGGTGTTTCC TGCAGGAGCG TGTTAGAGGG CACCTCCCAG 
GGCTCCTGTT TCGCCAGCGG CTCTGTGAGC ACTCAAGGAA GTCAAAAGAA AAACATCAAA 
ACCAAAGTCG ATATGGAAAA ACCAAGAGAA TCCTTGGGGG CCGAGGTCAA TGAAGATGTA 
CAAGAAGATG AAGAAGATAG AGTTGCTGAC ACTACTCTCC AGAGCCACAT GATAGAATCC 
AACTGCCAGA TGAGAACACT GGACAGCGGG ATTGGAACCT TTCCCCTCCC AGACTCAGGA 
AACCGCTCAG TGGGACGATA CATATGCCAG CAGGACTCTC CAGAGGACAC TGATGCCCTC 
CTGCCTCTCC AGCCAGCCGC TTCCATGGCA TCCTCTGCCA GAGCCCAAAC CCTTGACCGC 
GAAGTGCCTT CCTCCACAGA CAGCCAGAGC TCCCCGGAGT CCATGATCAT CCATTCCATA 
TCTGACCCCA GCATGATGGC CAGAGAGATG AGAGCCTCAC AGAGTCACCT TCCCAAGCCT 
ACCTCCTCAG GAAAACCTAG TTTTGAAAAG CAAAATGGAG CAGAGTCAAA GGCTCACACT 
GGCCCATCAT TCGAATATGC TGAGGATGCA GTGGCAAGTG AGCCCCTTTC AGGATGGAGG 
GGTGAAGATG CTACTACTGA GACCCAGAAC AGGTGCCCTG AGGAAGCAGA AATCCTTCAC 
AGACTTGAAA GTGACTCGAG GACAGTTAAT CGTCACCGTG CTCTAGTGAG GCAGGAACCG 
AAACAAGTTG AAGAAACAAA AGAAGATCCT GAGAATAGAT TATCTGAAAT TTCCCTGGAG 
TCATTCAATA AATTTAGCAG CAATACTGTG ATTTTATTAG GAAAAGAGAA GTCTCCAAGT 
GAGGTTGAAG GACAAAAGGA AGAGAAAGGC AAAAAGACAG AAGTGTCTTC GAGTAGCTCC 
GAACGGCCTG GGGTGGACCG CGTGGAGTCT CTGAGTGACT CTCTGTATGA CAGTTTCTCC 
TCTTGTGCAA GTCAAGCATC CAACGAGGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529333.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529396.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X12, mRNA.

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CloneID	OMu28060
Clone ID Related Accession (Same CDS sequence)	XM_006529333.3 , XM_006529333.4
Accession Version	XM_006529333.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529333.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG CAATCATCGC GGATCTGGGA TAAAAGACTA 
AGTATTGATT CTTCGCTCCC AAGTGGCTTC GCCAGCCCTG CAGATGAACT ACCTCCGACC 
CGTATCAAAG AAAGCCACAT TCTAGAGGGA CTGAGGAAGC TACAGAAGCG GAAAGTGTTC 
CTTGAATCAC CATCATTGGT GATAAGCAAA TGGGGTTATA AAGATTGCAT GAACTCGAAT 
GAAGGGATCT ATTCGCCAGG AATTAAAACC AGCAGCCTCA AGGAGTGTCC GCCCTGCCAA 
CCGTCTGGCA TGGGGATCCC CTGCCAGAAT CTCCATCAGA CATTCATCTA TGATGCAAAT 
TCTCATGAGG ATGCACATGA AGAACTCCCA TCCTCAGCCT TGCCACTAAC ATCTCCGAGC 
CAGGGCTACA GAATACAGGG CAGTAAATTG ACACACAGTG TTTCAGACAG TCTGTTTAAC 
TGGGAGCCAC ATAGGAAGTA CTTTGCAGAA GGTACAGCCT CAGTATATAC CAGAGAAATG 
CCTGAAAGGC TAGCTCGTTG TGCTAGCCAC TGCCCCTTGG AGAGGAAGCT ATGTCCCAGT 
ATACACCTGC CCTGGGTGCA AAGAGACAGG GACCCACACA GCCAAGTTCA TGGCCATGTG 
CCTCTGAACC TGCAGCTCTC AGATACCGAT GACAATGAGA CCCTAAACGA GCTACACATA 
GAGAGCAGTG ATGAGAGAAG CCCTTCAGAC CTGTCCTTGA CTGGGGACAC CGACAAGTCC 
ATGGAGAACC TAGAGGTGGG ATTCAGAAAG TCTGAAGGTG GCTCTCGGGA CATGGGAGAA 
GAAGCATGTC CCACCCACTT AGAGACTAGC CCGAAGGCAT TCAGTTTCAT CAAGCAACAA 
AGGGTTGTCA AGAGGACCTC TTCGGAAGAA TGTGTCACGG TCATATTTGA TGCAGAAAAT 
GGAGAGCCAG TGGAATTCAG CTCTCACCAG GCTGGAGTCG TCACTGTCAC CAGGGATGAA 
ATTTCCATCA GCTCAGCCCT TGCTGGGCCC TCAGAGGAAC TCACTGAACT TTCACCTCAG 
GAAATTACTC ACTTCCAGGC AGGAGCTACT GCAAGAGACT GTCCTTTTCT GAAGAGACCT 
GAAGAGGAGA CTGCGAAAAA CATTCCAAAA GATGAAAATC ATATTCTCAC AGCTCCGAAT 
GACTTCTTCA GCTCCCAGAC TATAATAACT CAGAATTCCC CAAGACCAAA GCTCGCCAAA 
CCCACACAGG GGGTAGCCTG GCAGAGTAAT TCTAGACCTG TTGGAAACAT GGGTGTCTAC 
ACAAAGCAAG GTCTGACAAA AATCCCTTCC AGAGGCAAGT CTTCACCTCA GAAATCCAAA 
CTTGTGGAGT CTATGCCCAC CATGCTGGTC CCCTCCAGTG CCCTAGTGAC TCCTGAAAAG 
ACACCAGCAT CCCCTATGGG GAAACTCTCA CAATGTAGAA AGGCAGAGAG CCCTGGTTCT 
CTCTGTGATG TACAGCCAGA ATCACACATT CCAAAACACC CTGCCCAGGT TCCTCATATC 
CCCAAAATTT CCAATAAGAA GAACTGGGTT CAGTGCCCCA GGAGTCAGAC TTCAGGGATC 
CAGTCACGGC ATCTCATGGG GAGAAGTGAC AGTAGTGAAA TGAGGTCCAA TGGCTGTGTC 
TCTGCGCCAC AGACAAGAAT GAGGTCTCCC TCCCCTCCAA CCCCTCCAGG GAGATCAGTG 
TCACTCCTGG TTAGACCCAG TTATGATTAC CCACCACTAC CATCACCTGC CAAACCTGAA 
ACTCAGGGCC CTGGAGACGC TGTAAGTCCA GGGTTGAAAT CCCCGCTGTT GAAAGGATCC 
TCTGCTCCTC TTGTCTCTTC CAGTCCAGTG ATGTCAGAAA TACAGAAGAA ACCATCTGTG 
GCCTCCAGAA AGTCTGCCTT CCCTCCAGCT CTTCCCTCAA CCCAAGCGGT AGTGCACACC 
AGATACCCTG AACACATAGC CTCCAGTCCA TTTGCTGTGA CGACTTCAGG GCCCCCAAAA 
GTCTCTCCAA AAAGAGGCAT CCCTAAACCT CCCCCTCACC ATGCACTAGG AACTGCCCAC 
ATGGACACAG ACTTACAGAC ACCCAAGACT TGTCCTTCAA GCTGTGAACT GCTAGAGGTG 
ACATCTTGTA AAAGCCTGTC TCCAGGGAGA AAAAGACAGT TGAGTGACAG CACTGTGATG 
CCCCACAGGC CTTCCTTCTC AGGAGTGAAT GAGTCCTTGA CACCTCAGGT TAACAGTCCA 
TCCTCCTCTT CCTCATCCTG TAGAAACCAA GGGTCCTCAC AAGGTGGCCA GAACTCTCCC 
GAGAAAGGAC CTAAAACTCG CCTCCCAGTA GGACTCAAAG TTCTCATGAA GTCTCCTCAG 
TTGCTCAAGA AAACTTCCTT GGTACCAGGG AAACAAGAAA AGGATAGTTT AAATGAAGCA 
TCTCGAAGCT GTGTAGCAGC AAACACGTCT AATCCTGAGG ACTCCAAAGA CCCTGCAAGT 
CTGGAGACCT CAAGAGATGA GAGAACCACA GCCTTACTAG GTGTGAGAGG TCCCGACAGT 
CTAGCTAGAG GACTTGCCTT AGACTCAACA ATGCCAGAAT CGTTAGAATG TAGCATCTCC 
AGAGATGATG GAAGAGATGG GGCAGACAGC AGAGCCATAA AAAGATCCTT CTCCTCCAGC 
AAGCCACACC TAAAGCCTGC TCTAGGCATG AATGGAGCCA AAGCTCGGAG TCAGAGCTTC 
AGCATCCACT TGGGAGAGAA GCCTTCCACA CCCCCAACAG AAGGGCCAGG CAAGATTCGA 
ACTCAGATTA TCACCAACAC GGCTGAAAGA GGCAACTCCC TCACCAGACA GAATATCTCA 
ATGGAAGGGT CCCCAAGCAA GACCCCCTCT GTCTCAACAT CTGATAATCT GCCCAATGCT 
GGAAGGCCCC TGGGAAACCC CTTTTCCAGG CAGAGTTCTC TCTGTAGCAC CGGAAGTTCT 
AGCAGCCAGC ACGGAAGCCC AAGCAAGTTA CCCTTGAGGA TGCTCACAAG GCCGGAAGGA 
CTCCTCACTC CTGGAGTGGA AGACCAACGG CTCTCTACTC AGGAAGAGTG TCCTGAAAGA 
GGTGTACATG AGCAATCTAG CAGTGACCAC CATAGGCATC CATCTCCTCT TTCAGACTGC 
CCTCAGTTCC TGCACGTTCC AGGGAGGATG CAGCGCCCAA GCAATTTGGA AACAAGTGGG 
ATATCTAAGC TAGAAACTTC TGGAAGGCAT CCAGATGCTT CTTCAACTGG GACTAGTGTC 
GTGAGCCCAG AAGCCCCCCT CTCACCCTCC ATCGAAGAAA AGGTCATGTT GTGCATTCAG 
GAGAATGTGG AAAAGGGCCA AGTGCAGACA AAGTCTGCCT CTGTGGAAGT GAAGCCCAGG 
GCAGGGCCTT CGTTCACCAG CTGGTTTGGT TTTCGAAAGA GCAGGCTTCC AGCTCTCAGT 
AGCAGGAGAA TGGAAGCTTC CAAGAGTAAA GTGGAAAAGA AAGACACAAA ACTGAAATCT 
GAGAGGAAAA AAGAAAAGAA GAAAGCTGAA GTACAATCAA AGATAGAAAA CGAACTGAGT 
AGGGGGACCA AGAAGGCAGA TGGTCAGAGT CCAGACGATG GTCTGCAAAG CTCAGAGAGT 
CTGAAAGTAT CCCAAGACAT TTATAACCAA ATGAAGTTGG AGCCAAGGAA TAGACCCAGC 
CCTGCTGTGT GCCCAACAAA AGATGCCTTC ATGACAGAAC TTCTAAACAG AGTTGATAAG 
AGAGCCTCGG TACAGAGAGA GAGTGGATCA AAGGGTGTTT CCTGCAGGAG CGTGTTAGAG 
GGCACCTCCC AGGGCTCCTG TTTCGCCAGC GGCTCTGTGA GCACTCAAGG AAGTCAAAAG 
AAAAACATCA AAACCAAAGT CGATATGGAA AAACCAAGAG AATCCTTGGG GGCCGAGGTC 
AATGAAGATG TACAAGAAGA TGAAGAAGAT AGAGTTGCTG ACACTACTCT CCAGAGCCAC 
ATGATAGAAT CCAACTGCCA GATGAGAACA CTGGACAGCG GGATTGGAAC CTTTCCCCTC 
CCAGACTCAG GAAACCGCTC AGTGGGACGA TACATATGCC AGCAGGACTC TCCAGAGGAC 
ACTGATGCCC TCCTGCCTCT CCAGCCAGCC GCTTCCATGG CATCCTCTGC CAGAGCCCAA 
ACCCTTGACC GCGAAGTGCC TTCCTCCACA GACAGCCAGA GCTCCCCGGA GTCCATGATC 
ATCCATTCCA TATCTGACCC CAGCATGATG GCCAGAGAGA TGAGAGCCTC ACAGAGTCAC 
CTTCCCAAGC CTACCTCCTC AGGAAAACCT AGTTTTGAAA AGCAAAATGG AGCAGAGTCA 
AAGGCTCACA CTGGCCCATC ATTCGAATAT GCTGAGGATG CAGTGGCAAG TGAGCCCCTT 
TCAGGATGGA GGGGTGAAGA TGCTACTACT GAGACCCAGG AACCGAAACA AGTTGAAGAA 
ACAAAAGAAG ATCCTGAGAA TAGATTATCT GAAATTTCCC TGGAGTCATT CAATAAATTT 
AGCAGCAATA CTGTGATTTT ATTAGGAAAA GAGAAGTCTC CAAGTGAGGT TGAAGGACAA 
AAGGAAGAGA AAGGCAAAAA GACAGAAGTG TCTTCGAGTA GCTCCGAACG GCCTGGGGTG 
GACCGCGTGG AGTCTCTGAG TGACTCTCTG TATGACAGTT TCTCCTCTTG TGCAAGTCAA 
GCATCCAACG AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529396.1
CDS	2650..8187
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_006529333.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529333.4

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG CAATCATCGC GGATCTGGGA TAAAAGACTA 
AGTATTGATT CTTCGCTCCC AAGTGGCTTC GCCAGCCCTG CAGATGAACT ACCTCCGACC 
CGTATCAAAG AAAGCCACAT TCTAGAGGGA CTGAGGAAGC TACAGAAGCG GAAAGTGTTC 
CTTGAATCAC CATCATTGGT GATAAGCAAA TGGGGTTATA AAGATTGCAT GAACTCGAAT 
GAAGGGATCT ATTCGCCAGG AATTAAAACC AGCAGCCTCA AGGAGTGTCC GCCCTGCCAA 
CCGTCTGGCA TGGGGATCCC CTGCCAGAAT CTCCATCAGA CATTCATCTA TGATGCAAAT 
TCTCATGAGG ATGCACATGA AGAACTCCCA TCCTCAGCCT TGCCACTAAC ATCTCCGAGC 
CAGGGCTACA GAATACAGGG CAGTAAATTG ACACACAGTG TTTCAGACAG TCTGTTTAAC 
TGGGAGCCAC ATAGGAAGTA CTTTGCAGAA GGTACAGCCT CAGTATATAC CAGAGAAATG 
CCTGAAAGGC TAGCTCGTTG TGCTAGCCAC TGCCCCTTGG AGAGGAAGCT ATGTCCCAGT 
ATACACCTGC CCTGGGTGCA AAGAGACAGG GACCCACACA GCCAAGTTCA TGGCCATGTG 
CCTCTGAACC TGCAGCTCTC AGATACCGAT GACAATGAGA CCCTAAACGA GCTACACATA 
GAGAGCAGTG ATGAGAGAAG CCCTTCAGAC CTGTCCTTGA CTGGGGACAC CGACAAGTCC 
ATGGAGAACC TAGAGGTGGG ATTCAGAAAG TCTGAAGGTG GCTCTCGGGA CATGGGAGAA 
GAAGCATGTC CCACCCACTT AGAGACTAGC CCGAAGGCAT TCAGTTTCAT CAAGCAACAA 
AGGGTTGTCA AGAGGACCTC TTCGGAAGAA TGTGTCACGG TCATATTTGA TGCAGAAAAT 
GGAGAGCCAG TGGAATTCAG CTCTCACCAG GCTGGAGTCG TCACTGTCAC CAGGGATGAA 
ATTTCCATCA GCTCAGCCCT TGCTGGGCCC TCAGAGGAAC TCACTGAACT TTCACCTCAG 
GAAATTACTC ACTTCCAGGC AGGAGCTACT GCAAGAGACT GTCCTTTTCT GAAGAGACCT 
GAAGAGGAGA CTGCGAAAAA CATTCCAAAA GATGAAAATC ATATTCTCAC AGCTCCGAAT 
GACTTCTTCA GCTCCCAGAC TATAATAACT CAGAATTCCC CAAGACCAAA GCTCGCCAAA 
CCCACACAGG GGGTAGCCTG GCAGAGTAAT TCTAGACCTG TTGGAAACAT GGGTGTCTAC 
ACAAAGCAAG GTCTGACAAA AATCCCTTCC AGAGGCAAGT CTTCACCTCA GAAATCCAAA 
CTTGTGGAGT CTATGCCCAC CATGCTGGTC CCCTCCAGTG CCCTAGTGAC TCCTGAAAAG 
ACACCAGCAT CCCCTATGGG GAAACTCTCA CAATGTAGAA AGGCAGAGAG CCCTGGTTCT 
CTCTGTGATG TACAGCCAGA ATCACACATT CCAAAACACC CTGCCCAGGT TCCTCATATC 
CCCAAAATTT CCAATAAGAA GAACTGGGTT CAGTGCCCCA GGAGTCAGAC TTCAGGGATC 
CAGTCACGGC ATCTCATGGG GAGAAGTGAC AGTAGTGAAA TGAGGTCCAA TGGCTGTGTC 
TCTGCGCCAC AGACAAGAAT GAGGTCTCCC TCCCCTCCAA CCCCTCCAGG GAGATCAGTG 
TCACTCCTGG TTAGACCCAG TTATGATTAC CCACCACTAC CATCACCTGC CAAACCTGAA 
ACTCAGGGCC CTGGAGACGC TGTAAGTCCA GGGTTGAAAT CCCCGCTGTT GAAAGGATCC 
TCTGCTCCTC TTGTCTCTTC CAGTCCAGTG ATGTCAGAAA TACAGAAGAA ACCATCTGTG 
GCCTCCAGAA AGTCTGCCTT CCCTCCAGCT CTTCCCTCAA CCCAAGCGGT AGTGCACACC 
AGATACCCTG AACACATAGC CTCCAGTCCA TTTGCTGTGA CGACTTCAGG GCCCCCAAAA 
GTCTCTCCAA AAAGAGGCAT CCCTAAACCT CCCCCTCACC ATGCACTAGG AACTGCCCAC 
ATGGACACAG ACTTACAGAC ACCCAAGACT TGTCCTTCAA GCTGTGAACT GCTAGAGGTG 
ACATCTTGTA AAAGCCTGTC TCCAGGGAGA AAAAGACAGT TGAGTGACAG CACTGTGATG 
CCCCACAGGC CTTCCTTCTC AGGAGTGAAT GAGTCCTTGA CACCTCAGGT TAACAGTCCA 
TCCTCCTCTT CCTCATCCTG TAGAAACCAA GGGTCCTCAC AAGGTGGCCA GAACTCTCCC 
GAGAAAGGAC CTAAAACTCG CCTCCCAGTA GGACTCAAAG TTCTCATGAA GTCTCCTCAG 
TTGCTCAAGA AAACTTCCTT GGTACCAGGG AAACAAGAAA AGGATAGTTT AAATGAAGCA 
TCTCGAAGCT GTGTAGCAGC AAACACGTCT AATCCTGAGG ACTCCAAAGA CCCTGCAAGT 
CTGGAGACCT CAAGAGATGA GAGAACCACA GCCTTACTAG GTGTGAGAGG TCCCGACAGT 
CTAGCTAGAG GACTTGCCTT AGACTCAACA ATGCCAGAAT CGTTAGAATG TAGCATCTCC 
AGAGATGATG GAAGAGATGG GGCAGACAGC AGAGCCATAA AAAGATCCTT CTCCTCCAGC 
AAGCCACACC TAAAGCCTGC TCTAGGCATG AATGGAGCCA AAGCTCGGAG TCAGAGCTTC 
AGCATCCACT TGGGAGAGAA GCCTTCCACA CCCCCAACAG AAGGGCCAGG CAAGATTCGA 
ACTCAGATTA TCACCAACAC GGCTGAAAGA GGCAACTCCC TCACCAGACA GAATATCTCA 
ATGGAAGGGT CCCCAAGCAA GACCCCCTCT GTCTCAACAT CTGATAATCT GCCCAATGCT 
GGAAGGCCCC TGGGAAACCC CTTTTCCAGG CAGAGTTCTC TCTGTAGCAC CGGAAGTTCT 
AGCAGCCAGC ACGGAAGCCC AAGCAAGTTA CCCTTGAGGA TGCTCACAAG GCCGGAAGGA 
CTCCTCACTC CTGGAGTGGA AGACCAACGG CTCTCTACTC AGGAAGAGTG TCCTGAAAGA 
GGTGTACATG AGCAATCTAG CAGTGACCAC CATAGGCATC CATCTCCTCT TTCAGACTGC 
CCTCAGTTCC TGCACGTTCC AGGGAGGATG CAGCGCCCAA GCAATTTGGA AACAAGTGGG 
ATATCTAAGC TAGAAACTTC TGGAAGGCAT CCAGATGCTT CTTCAACTGG GACTAGTGTC 
GTGAGCCCAG AAGCCCCCCT CTCACCCTCC ATCGAAGAAA AGGTCATGTT GTGCATTCAG 
GAGAATGTGG AAAAGGGCCA AGTGCAGACA AAGTCTGCCT CTGTGGAAGT GAAGCCCAGG 
GCAGGGCCTT CGTTCACCAG CTGGTTTGGT TTTCGAAAGA GCAGGCTTCC AGCTCTCAGT 
AGCAGGAGAA TGGAAGCTTC CAAGAGTAAA GTGGAAAAGA AAGACACAAA ACTGAAATCT 
GAGAGGAAAA AAGAAAAGAA GAAAGCTGAA GTACAATCAA AGATAGAAAA CGAACTGAGT 
AGGGGGACCA AGAAGGCAGA TGGTCAGAGT CCAGACGATG GTCTGCAAAG CTCAGAGAGT 
CTGAAAGTAT CCCAAGACAT TTATAACCAA ATGAAGTTGG AGCCAAGGAA TAGACCCAGC 
CCTGCTGTGT GCCCAACAAA AGATGCCTTC ATGACAGAAC TTCTAAACAG AGTTGATAAG 
AGAGCCTCGG TACAGAGAGA GAGTGGATCA AAGGGTGTTT CCTGCAGGAG CGTGTTAGAG 
GGCACCTCCC AGGGCTCCTG TTTCGCCAGC GGCTCTGTGA GCACTCAAGG AAGTCAAAAG 
AAAAACATCA AAACCAAAGT CGATATGGAA AAACCAAGAG AATCCTTGGG GGCCGAGGTC 
AATGAAGATG TACAAGAAGA TGAAGAAGAT AGAGTTGCTG ACACTACTCT CCAGAGCCAC 
ATGATAGAAT CCAACTGCCA GATGAGAACA CTGGACAGCG GGATTGGAAC CTTTCCCCTC 
CCAGACTCAG GAAACCGCTC AGTGGGACGA TACATATGCC AGCAGGACTC TCCAGAGGAC 
ACTGATGCCC TCCTGCCTCT CCAGCCAGCC GCTTCCATGG CATCCTCTGC CAGAGCCCAA 
ACCCTTGACC GCGAAGTGCC TTCCTCCACA GACAGCCAGA GCTCCCCGGA GTCCATGATC 
ATCCATTCCA TATCTGACCC CAGCATGATG GCCAGAGAGA TGAGAGCCTC ACAGAGTCAC 
CTTCCCAAGC CTACCTCCTC AGGAAAACCT AGTTTTGAAA AGCAAAATGG AGCAGAGTCA 
AAGGCTCACA CTGGCCCATC ATTCGAATAT GCTGAGGATG CAGTGGCAAG TGAGCCCCTT 
TCAGGATGGA GGGGTGAAGA TGCTACTACT GAGACCCAGG AACCGAAACA AGTTGAAGAA 
ACAAAAGAAG ATCCTGAGAA TAGATTATCT GAAATTTCCC TGGAGTCATT CAATAAATTT 
AGCAGCAATA CTGTGATTTT ATTAGGAAAA GAGAAGTCTC CAAGTGAGGT TGAAGGACAA 
AAGGAAGAGA AAGGCAAAAA GACAGAAGTG TCTTCGAGTA GCTCCGAACG GCCTGGGGTG 
GACCGCGTGG AGTCTCTGAG TGACTCTCTG TATGACAGTT TCTCCTCTTG TGCAAGTCAA 
GCATCCAACG AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529335.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529398.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X14, mRNA.

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CloneID	OMu28061
Clone ID Related Accession (Same CDS sequence)	XM_006529334.3 , XM_006529335.4 , XM_006529335.3 , XM_006529334.4
Accession Version	XM_006529335.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529335.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGTAACCATG 
GGACATGTCA TCTTACAGGC GGTGATAACA CCTCACCCTG AGTCCCCTGT GGAAAGAAAG 
AGCCATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529398.1
CDS	2650..8181
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQVTMGHVILQAVITPHPESPVERKSHD

Target ORF information:

RefSeq Version	XM_006529335.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529335.4

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGTAACCATG 
GGACATGTCA TCTTACAGGC GGTGATAACA CCTCACCCTG AGTCCCCTGT GGAAAGAAAG 
AGCCATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529334.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529397.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X13, mRNA.

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CloneID	OMu28061
Clone ID Related Accession (Same CDS sequence)	XM_006529334.3 , XM_006529335.4 , XM_006529335.3 , XM_006529334.4
Accession Version	XM_006529334.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529334.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGTAACCATG 
GGACATGTCA TCTTACAGGC GGTGATAACA CCTCACCCTG AGTCCCCTGT GGAAAGAAAG 
AGCCATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529397.1
CDS	2650..8181
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQVTMGHVILQAVITPHPESPVERKSHD

Target ORF information:

RefSeq Version	XM_006529334.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529334.4

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGTAACCATG 
GGACATGTCA TCTTACAGGC GGTGATAACA CCTCACCCTG AGTCCCCTGT GGAAAGAAAG 
AGCCATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529336.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529399.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X15, mRNA.

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CloneID	OMu28062
Clone ID Related Accession (Same CDS sequence)	XM_006529336.3 , XM_006529336.4
Accession Version	XM_006529336.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529336.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGCGGTGATA 
ACACCTCACC CTGAGTCCCC TGTGGAAAGA AAGAGCCATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529399.1
CDS	2649..8153
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQAVITPHPESPVERKSHD

Target ORF information:

RefSeq Version	XM_006529336.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529336.4

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGCGGTGATA 
ACACCTCACC CTGAGTCCCC TGTGGAAAGA AAGAGCCATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_011247958.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246260.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X4, mRNA.

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CloneID	OMu72729
Clone ID Related Accession (Same CDS sequence)	XM_011247958.2 , XM_011247958.3
Accession Version	XM_011247958.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247958.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTA ACAAATGCAT CGAGCACCTG CTGGCACAAC TCAGGGAGCA ACACAGAAGT 
CTCTGGCGGG AGAAGCTGGC TGTGGCCAGG CTGCAGAGAG AAGTTGCCCA AAGGACAAGC 
CAAGGGGCCA TGCACGAGAA GCTGATACAT GAGTTGGAAG AGGAACGGCA CTTGCGGCTA 
CAGAGTGAGG AGCGGCTGCG GGAGGTGACC CTGGAGTCAG AGCGCAACAG GATCCAGATG 
CTCGGCCTGC AGCAGCAGTT TTCCAGAATG GAAGAAACTG TCCGAAGTCT GTTGCAGAGC 
CAAGGATCTC CAGAGCAGAA AAAGGAAGAA CCTGCCAAGA TCACAGCCTA CCAGGAAGGA 
CTACCTGATG AAGAGAGAAA AGAGCAAGCA GCACTGGGTG ATCTCCATGT GGTGGCAGCT 
GATGAGGACT CAAGAAGCGA GCACAGTAGT GTGGAGGAGG GAAAGGAAGA CAGCAGACTG 
CTCCTGAAGA GACTGAAGGC CCTGGAGGCG GAGAATTCAG CACTGGCACT GGAAAACGAG 
AATCAGAGAG AACAGTATGA GCGATGTCTT GATGAGGTAG CCAACCAAGT TGTTCGAGCT 
CTACTCACTC AAAAGGATCT GAGGGAGGAG TGTGTAAAGT TGAAAACAAG AGTGTTTGAC 
TTGGAGCAAC AGAACCGAGC ACTCAGCCTC CTGTTCCAAC AGCGAGTCAG ACCAGCTTCT 
GATGTGCTTC TCCAGGAACA CCTCCAGAAT GCAAAATCAG GAACCCCTGC CTTGAAACAC 
TCTGGCTCAG GGGTTGTCAT CCCTGATCAC CTCTGTCCTC GCAACAGCTG CAGCAGTGGC 
AGTGAGCTAT CGCTGTCAAG CACCTGCAGC GAGTACTCCA GTGGCTCCTC CTACACGTGG 
CAAGACAGGA AGCTCATTGG GAAAAGGCAA TCATCGCGGA TCTGGGATAA AAGACTAAGT 
ATTGATTCTT CGCTCCCAAG TGGCTTCGCC AGCCCTGCAG ATGAACTACC TCCGACCCGT 
ATCAAAGAAA GCCACATTCT AGAGGGACTG AGGAAGCTAC AGAAGCGGAA AGTGTTCCTT 
GAATCACCAT CATTGGTGAT AAGCAAATGG GGTTATAAAG ATTGCATGAA CTCGAATGAA 
GGGATCTATT CGCCAGGAAT TAAAACCAGC AGCCTCAAGG AGTGTCCGCC CTGCCAACCG 
TCTGGCATGG GGATCCCCTG CCAGAATCTC CATCAGACAT TCATCTATGA TGCAAATTCT 
CATGAGGATG CACATGAAGA ACTCCCATCC TCAGCCTTGC CACTAACATC TCCGAGCCAG 
GGCTACAGAA TACAGGGCAG TAAATTGACA CACAGTGTTT CAGACAGTCT GTTTAACTGG 
GAGCCACATA GGAAGTACTT TGCAGAAGGT ACAGCCTCAG TATATACCAG AGAAATGCCT 
GAAAGGCTAG CTCGTTGTGC TAGCCACTGC CCCTTGGAGA GGAAGCTATG TCCCAGTATA 
CACCTGCCCT GGGTGCAAAG AGACAGGGAC CCACACAGCC AAGTTCATGG CCATGTGCCT 
CTGAACCTGC AGCTCTCAGA TACCGATGAC AATGAGACCC TAAACGAGCT ACACATAGAG 
AGCAGTGATG AGAGAAGCCC TTCAGACCTG TCCTTGACTG GGGACACCGA CAAGTCCATG 
GAGAACCTAG AGGTGGGATT CAGAAAGTCT GAAGGTGGCT CTCGGGACAT GGGAGAAGAA 
GCATGTCCCA CCCACTTAGA GACTAGCCCG AAGGCATTCA GTTTCATCAA GCAACAAAGG 
GTTGTCAAGA GGACCTCTTC GGAAGAATGT GTCACGGTCA TATTTGATGC AGAAAATGGA 
GAGCCAGTGG AATTCAGCTC TCACCAGGCT GGAGTCGTCA CTGTCACCAG GGATGAAATT 
TCCATCAGCT CAGCCCTTGC TGGGCCCTCA GAGGAACTCA CTGAACTTTC ACCTCAGGAA 
ATTACTCACT TCCAGGCAGG AGCTACTGCA AGAGACTGTC CTTTTCTGAA GAGACCTGAA 
GAGGAGACTG CGAAAAACAT TCCAAAAGAT GAAAATCATA TTCTCACAGC TCCGAATGAC 
TTCTTCAGCT CCCAGACTAT AATAACTCAG AATTCCCCAA GACCAAAGCT CGCCAAACCC 
ACACAGGGGG TAGCCTGGCA GAGTAATTCT AGACCTGTTG GAAACATGGG TGTCTACACA 
AAGCAAGGTC TGACAAAAAT CCCTTCCAGA GGCAAGTCTT CACCTCAGAA ATCCAAACTT 
GTGGAGTCTA TGCCCACCAT GCTGGTCCCC TCCAGTGCCC TAGTGACTCC TGAAAAGACA 
CCAGCATCCC CTATGGGGAA ACTCTCACAA TGTAGAAAGG CAGAGAGCCC TGGTTCTCTC 
TGTGATGTAC AGCCAGAATC ACACATTCCA AAACACCCTG CCCAGGTTCC TCATATCCCC 
AAAATTTCCA ATAAGAAGAA CTGGGTTCAG TGCCCCAGGA GTCAGACTTC AGGGATCCAG 
TCACGGCATC TCATGGGGAG AAGTGACAGT AGTGAAATGA GGTCCAATGG CTGTGTCTCT 
GCGCCACAGA CAAGAATGAG GTCTCCCTCC CCTCCAACCC CTCCAGGGAG ATCAGTGTCA 
CTCCTGGTTA GACCCAGTTA TGATTACCCA CCACTACCAT CACCTGCCAA ACCTGAAACT 
CAGGGCCCTG GAGACGCTGT AAGTCCAGGG TTGAAATCCC CGCTGTTGAA AGGATCCTCT 
GCTCCTCTTG TCTCTTCCAG TCCAGTGATG TCAGAAATAC AGAAGAAACC ATCTGTGGCC 
TCCAGAAAGT CTGCCTTCCC TCCAGCTCTT CCCTCAACCC AAGCGGTAGT GCACACCAGA 
TACCCTGAAC ACATAGCCTC CAGTCCATTT GCTGTGACGA CTTCAGGGCC CCCAAAAGTC 
TCTCCAAAAA GAGGCATCCC TAAACCTCCC CCTCACCATG CACTAGGAAC TGCCCACATG 
GACACAGACT TACAGACACC CAAGACTTGT CCTTCAAGCT GTGAACTGCT AGAGGTGACA 
TCTTGTAAAA GCCTGTCTCC AGGGAGAAAA AGACAGTTGA GTGACAGCAC TGTGATGCCC 
CACAGGCCTT CCTTCTCAGG AGTGAATGAG TCCTTGACAC CTCAGGTTAA CAGTCCATCC 
TCCTCTTCCT CATCCTGTAG AAACCAAGGG TCCTCACAAG GTGGCCAGAA CTCTCCCGAG 
AAAGGACCTA AAACTCGCCT CCCAGTAGGA CTCAAAGTTC TCATGAAGTC TCCTCAGTTG 
CTCAAGAAAA CTTCCTTGGT ACCAGGGAAA CAAGAAAAGG ATAGTTTAAA TGAAGCATCT 
CGAAGCTGTG TAGCAGCAAA CACGTCTAAT CCTGAGGACT CCAAAGACCC TGCAAGTCTG 
GAGACCTCAA GAGATGAGAG AACCACAGCC TTACTAGGTG TGAGAGGTCC CGACAGTCTA 
GCTAGAGGAC TTGCCTTAGA CTCAACAATG CCAGAATCGT TAGAATGTAG CATCTCCAGA 
GATGATGGAA GAGATGGGGC AGACAGCAGA GCCATAAAAA GATCCTTCTC CTCCAGCAAG 
CCACACCTAA AGCCTGCTCT AGGCATGAAT GGAGCCAAAG CTCGGAGTCA GAGCTTCAGC 
ATCCACTTGG GAGAGAAGCC TTCCACACCC CCAACAGAAG GGCCAGGCAA GATTCGAACT 
CAGATTATCA CCAACACGGC TGAAAGAGGC AACTCCCTCA CCAGACAGAA TATCTCAATG 
GAAGGGTCCC CAAGCAAGAC CCCCTCTGTC TCAACATCTG ATAATCTGCC CAATGCTGGA 
AGGCCCCTGG GAAACCCCTT TTCCAGGCAG AGTTCTCTCT GTAGCACCGG AAGTTCTAGC 
AGCCAGCACG GAAGCCCAAG CAAGTTACCC TTGAGGATGC TCACAAGGCC GGAAGGACTC 
CTCACTCCTG GAGTGGAAGA CCAACGGCTC TCTACTCAGG AAGAGTGTCC TGAAAGAGGT 
GTACATGAGC AATCTAGCAG TGACCACCAT AGGCATCCAT CTCCTCTTTC AGACTGCCCT 
CAGTTCCTGC ACGTTCCAGG GAGGATGCAG CGCCCAAGCA ATTTGGAAAC AAGTGGGATA 
TCTAAGCTAG AAACTTCTGG AAGGCATCCA GATGCTTCTT CAACTGGGAC TAGTGTCGTG 
AGCCCAGAAG CCCCCCTCTC ACCCTCCATC GAAGAAAAGG TCATGTTGTG CATTCAGGAG 
AATGTGGAAA AGGGCCAAGT GCAGACAAAG TCTGCCTCTG TGGAAGTGAA GCCCAGGGCA 
GGGCCTTCGT TCACCAGCTG GTTTGGTTTT CGAAAGAGCA GGCTTCCAGC TCTCAGTAGC 
AGGAGAATGG AAGCTTCCAA GAGTAAAGTG GAAAAGAAAG ACACAAAACT GAAATCTGAG 
AGGAAAAAAG AAAAGAAGAA AGCTGAAGTA CAATCAAAGA TAGAAAACGA ACTGAGTAGG 
GGGACCAAGA AGGCAGATGG TCAGAGTCCA GACGATGGTC TGCAAAGCTC AGAGAGTCTG 
AAAGTATCCC AAGACATTTA TAACCAAATG AAGTTGGAGC CAAGGAATAG ACCCAGCCCT 
GCTGTGTGCC CAACAAAAGA TGCCTTCATG ACAGAACTTC TAAACAGAGT TGATAAGAGA 
GCCTCGGTAC AGAGAGAGAG TGGATCAAAG GGTGTTTCCT GCAGGAGCGT GTTAGAGGGC 
ACCTCCCAGG GCTCCTGTTT CGCCAGCGGC TCTGTGAGCA CTCAAGGAAG TCAAAAGAAA 
AACATCAAAA CCAAAGTCGA TATGGAAAAA CCAAGAGAAT CCTTGGGGGC CGAGGTCAAT 
GAAGATGTAC AAGAAGATGA AGAAGATAGA GTTGCTGACA CTACTCTCCA GAGCCACATG 
ATAGAATCCA ACTGCCAGAT GAGAACACTG GACAGCGGGA TTGGAACCTT TCCCCTCCCA 
GACTCAGGAA ACCGCTCAGT GGGACGATAC ATATGCCAGC AGGACTCTCC AGAGGACACT 
GATGCCCTCC TGCCTCTCCA GCCAGCCGCT TCCATGGCAT CCTCTGCCAG AGCCCAAACC 
CTTGACCGCG AAGTGCCTTC CTCCACAGAC AGCCAGAGCT CCCCGGAGTC CATGATCATC 
CATTCCATAT CTGACCCCAG CATGATGGCC AGAGAGATGA GAGCCTCACA GAGTCACCTT 
CCCAAGCCTA CCTCCTCAGG AAAACCTAGT TTTGAAAAGC AAAATGGAGC AGAGTCAAAG 
GCTCACACTG GCCCATCATT CGAATATGCT GAGGATGCAG TGGCAAGTGA GCCCCTTTCA 
GGATGGAGGG GTGAAGATGC TACTACTGAG ACCCAGAACA GGTGCCCTGA GGAAGCAGAA 
ATCCTTCACA GACTTGAAAG TGACTCGAGG ACAGTTAATC GTCACCGTGC TCTAGTGAGG 
CAGGAACCGA AACAAGTTGA AGAAACAAAA GAAGATCCTG AGAATAGATT ATCTGAAATT 
TCCCTGGAGT CATTCAATAA ATTTAGCAGC AATACTGTGA TTTTATTAGG AAAAGAGAAG 
TCTCCAAGTG AGGTTGAAGG ACAAAAGGAA GAGAAAGGCA AAAAGACAGA AGTGTCTTCG 
AGTAGCTCCG AACGGCCTGG GGTGGACCGC GTGGAGTCTC TGAGTGACTC TCTGTATGAC 
AGTTTCTCCT CTTGTGCAAG TCAAGCATCC AACGAGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246260.1
CDS	21751..27492
Translation	MDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_011247958.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247958.3

ORF Insert Sequence:

ATGGACTCTA ACAAATGCAT CGAGCACCTG CTGGCACAAC TCAGGGAGCA ACACAGAAGT 
CTCTGGCGGG AGAAGCTGGC TGTGGCCAGG CTGCAGAGAG AAGTTGCCCA AAGGACAAGC 
CAAGGGGCCA TGCACGAGAA GCTGATACAT GAGTTGGAAG AGGAACGGCA CTTGCGGCTA 
CAGAGTGAGG AGCGGCTGCG GGAGGTGACC CTGGAGTCAG AGCGCAACAG GATCCAGATG 
CTCGGCCTGC AGCAGCAGTT TTCCAGAATG GAAGAAACTG TCCGAAGTCT GTTGCAGAGC 
CAAGGATCTC CAGAGCAGAA AAAGGAAGAA CCTGCCAAGA TCACAGCCTA CCAGGAAGGA 
CTACCTGATG AAGAGAGAAA AGAGCAAGCA GCACTGGGTG ATCTCCATGT GGTGGCAGCT 
GATGAGGACT CAAGAAGCGA GCACAGTAGT GTGGAGGAGG GAAAGGAAGA CAGCAGACTG 
CTCCTGAAGA GACTGAAGGC CCTGGAGGCG GAGAATTCAG CACTGGCACT GGAAAACGAG 
AATCAGAGAG AACAGTATGA GCGATGTCTT GATGAGGTAG CCAACCAAGT TGTTCGAGCT 
CTACTCACTC AAAAGGATCT GAGGGAGGAG TGTGTAAAGT TGAAAACAAG AGTGTTTGAC 
TTGGAGCAAC AGAACCGAGC ACTCAGCCTC CTGTTCCAAC AGCGAGTCAG ACCAGCTTCT 
GATGTGCTTC TCCAGGAACA CCTCCAGAAT GCAAAATCAG GAACCCCTGC CTTGAAACAC 
TCTGGCTCAG GGGTTGTCAT CCCTGATCAC CTCTGTCCTC GCAACAGCTG CAGCAGTGGC 
AGTGAGCTAT CGCTGTCAAG CACCTGCAGC GAGTACTCCA GTGGCTCCTC CTACACGTGG 
CAAGACAGGA AGCTCATTGG GAAAAGGCAA TCATCGCGGA TCTGGGATAA AAGACTAAGT 
ATTGATTCTT CGCTCCCAAG TGGCTTCGCC AGCCCTGCAG ATGAACTACC TCCGACCCGT 
ATCAAAGAAA GCCACATTCT AGAGGGACTG AGGAAGCTAC AGAAGCGGAA AGTGTTCCTT 
GAATCACCAT CATTGGTGAT AAGCAAATGG GGTTATAAAG ATTGCATGAA CTCGAATGAA 
GGGATCTATT CGCCAGGAAT TAAAACCAGC AGCCTCAAGG AGTGTCCGCC CTGCCAACCG 
TCTGGCATGG GGATCCCCTG CCAGAATCTC CATCAGACAT TCATCTATGA TGCAAATTCT 
CATGAGGATG CACATGAAGA ACTCCCATCC TCAGCCTTGC CACTAACATC TCCGAGCCAG 
GGCTACAGAA TACAGGGCAG TAAATTGACA CACAGTGTTT CAGACAGTCT GTTTAACTGG 
GAGCCACATA GGAAGTACTT TGCAGAAGGT ACAGCCTCAG TATATACCAG AGAAATGCCT 
GAAAGGCTAG CTCGTTGTGC TAGCCACTGC CCCTTGGAGA GGAAGCTATG TCCCAGTATA 
CACCTGCCCT GGGTGCAAAG AGACAGGGAC CCACACAGCC AAGTTCATGG CCATGTGCCT 
CTGAACCTGC AGCTCTCAGA TACCGATGAC AATGAGACCC TAAACGAGCT ACACATAGAG 
AGCAGTGATG AGAGAAGCCC TTCAGACCTG TCCTTGACTG GGGACACCGA CAAGTCCATG 
GAGAACCTAG AGGTGGGATT CAGAAAGTCT GAAGGTGGCT CTCGGGACAT GGGAGAAGAA 
GCATGTCCCA CCCACTTAGA GACTAGCCCG AAGGCATTCA GTTTCATCAA GCAACAAAGG 
GTTGTCAAGA GGACCTCTTC GGAAGAATGT GTCACGGTCA TATTTGATGC AGAAAATGGA 
GAGCCAGTGG AATTCAGCTC TCACCAGGCT GGAGTCGTCA CTGTCACCAG GGATGAAATT 
TCCATCAGCT CAGCCCTTGC TGGGCCCTCA GAGGAACTCA CTGAACTTTC ACCTCAGGAA 
ATTACTCACT TCCAGGCAGG AGCTACTGCA AGAGACTGTC CTTTTCTGAA GAGACCTGAA 
GAGGAGACTG CGAAAAACAT TCCAAAAGAT GAAAATCATA TTCTCACAGC TCCGAATGAC 
TTCTTCAGCT CCCAGACTAT AATAACTCAG AATTCCCCAA GACCAAAGCT CGCCAAACCC 
ACACAGGGGG TAGCCTGGCA GAGTAATTCT AGACCTGTTG GAAACATGGG TGTCTACACA 
AAGCAAGGTC TGACAAAAAT CCCTTCCAGA GGCAAGTCTT CACCTCAGAA ATCCAAACTT 
GTGGAGTCTA TGCCCACCAT GCTGGTCCCC TCCAGTGCCC TAGTGACTCC TGAAAAGACA 
CCAGCATCCC CTATGGGGAA ACTCTCACAA TGTAGAAAGG CAGAGAGCCC TGGTTCTCTC 
TGTGATGTAC AGCCAGAATC ACACATTCCA AAACACCCTG CCCAGGTTCC TCATATCCCC 
AAAATTTCCA ATAAGAAGAA CTGGGTTCAG TGCCCCAGGA GTCAGACTTC AGGGATCCAG 
TCACGGCATC TCATGGGGAG AAGTGACAGT AGTGAAATGA GGTCCAATGG CTGTGTCTCT 
GCGCCACAGA CAAGAATGAG GTCTCCCTCC CCTCCAACCC CTCCAGGGAG ATCAGTGTCA 
CTCCTGGTTA GACCCAGTTA TGATTACCCA CCACTACCAT CACCTGCCAA ACCTGAAACT 
CAGGGCCCTG GAGACGCTGT AAGTCCAGGG TTGAAATCCC CGCTGTTGAA AGGATCCTCT 
GCTCCTCTTG TCTCTTCCAG TCCAGTGATG TCAGAAATAC AGAAGAAACC ATCTGTGGCC 
TCCAGAAAGT CTGCCTTCCC TCCAGCTCTT CCCTCAACCC AAGCGGTAGT GCACACCAGA 
TACCCTGAAC ACATAGCCTC CAGTCCATTT GCTGTGACGA CTTCAGGGCC CCCAAAAGTC 
TCTCCAAAAA GAGGCATCCC TAAACCTCCC CCTCACCATG CACTAGGAAC TGCCCACATG 
GACACAGACT TACAGACACC CAAGACTTGT CCTTCAAGCT GTGAACTGCT AGAGGTGACA 
TCTTGTAAAA GCCTGTCTCC AGGGAGAAAA AGACAGTTGA GTGACAGCAC TGTGATGCCC 
CACAGGCCTT CCTTCTCAGG AGTGAATGAG TCCTTGACAC CTCAGGTTAA CAGTCCATCC 
TCCTCTTCCT CATCCTGTAG AAACCAAGGG TCCTCACAAG GTGGCCAGAA CTCTCCCGAG 
AAAGGACCTA AAACTCGCCT CCCAGTAGGA CTCAAAGTTC TCATGAAGTC TCCTCAGTTG 
CTCAAGAAAA CTTCCTTGGT ACCAGGGAAA CAAGAAAAGG ATAGTTTAAA TGAAGCATCT 
CGAAGCTGTG TAGCAGCAAA CACGTCTAAT CCTGAGGACT CCAAAGACCC TGCAAGTCTG 
GAGACCTCAA GAGATGAGAG AACCACAGCC TTACTAGGTG TGAGAGGTCC CGACAGTCTA 
GCTAGAGGAC TTGCCTTAGA CTCAACAATG CCAGAATCGT TAGAATGTAG CATCTCCAGA 
GATGATGGAA GAGATGGGGC AGACAGCAGA GCCATAAAAA GATCCTTCTC CTCCAGCAAG 
CCACACCTAA AGCCTGCTCT AGGCATGAAT GGAGCCAAAG CTCGGAGTCA GAGCTTCAGC 
ATCCACTTGG GAGAGAAGCC TTCCACACCC CCAACAGAAG GGCCAGGCAA GATTCGAACT 
CAGATTATCA CCAACACGGC TGAAAGAGGC AACTCCCTCA CCAGACAGAA TATCTCAATG 
GAAGGGTCCC CAAGCAAGAC CCCCTCTGTC TCAACATCTG ATAATCTGCC CAATGCTGGA 
AGGCCCCTGG GAAACCCCTT TTCCAGGCAG AGTTCTCTCT GTAGCACCGG AAGTTCTAGC 
AGCCAGCACG GAAGCCCAAG CAAGTTACCC TTGAGGATGC TCACAAGGCC GGAAGGACTC 
CTCACTCCTG GAGTGGAAGA CCAACGGCTC TCTACTCAGG AAGAGTGTCC TGAAAGAGGT 
GTACATGAGC AATCTAGCAG TGACCACCAT AGGCATCCAT CTCCTCTTTC AGACTGCCCT 
CAGTTCCTGC ACGTTCCAGG GAGGATGCAG CGCCCAAGCA ATTTGGAAAC AAGTGGGATA 
TCTAAGCTAG AAACTTCTGG AAGGCATCCA GATGCTTCTT CAACTGGGAC TAGTGTCGTG 
AGCCCAGAAG CCCCCCTCTC ACCCTCCATC GAAGAAAAGG TCATGTTGTG CATTCAGGAG 
AATGTGGAAA AGGGCCAAGT GCAGACAAAG TCTGCCTCTG TGGAAGTGAA GCCCAGGGCA 
GGGCCTTCGT TCACCAGCTG GTTTGGTTTT CGAAAGAGCA GGCTTCCAGC TCTCAGTAGC 
AGGAGAATGG AAGCTTCCAA GAGTAAAGTG GAAAAGAAAG ACACAAAACT GAAATCTGAG 
AGGAAAAAAG AAAAGAAGAA AGCTGAAGTA CAATCAAAGA TAGAAAACGA ACTGAGTAGG 
GGGACCAAGA AGGCAGATGG TCAGAGTCCA GACGATGGTC TGCAAAGCTC AGAGAGTCTG 
AAAGTATCCC AAGACATTTA TAACCAAATG AAGTTGGAGC CAAGGAATAG ACCCAGCCCT 
GCTGTGTGCC CAACAAAAGA TGCCTTCATG ACAGAACTTC TAAACAGAGT TGATAAGAGA 
GCCTCGGTAC AGAGAGAGAG TGGATCAAAG GGTGTTTCCT GCAGGAGCGT GTTAGAGGGC 
ACCTCCCAGG GCTCCTGTTT CGCCAGCGGC TCTGTGAGCA CTCAAGGAAG TCAAAAGAAA 
AACATCAAAA CCAAAGTCGA TATGGAAAAA CCAAGAGAAT CCTTGGGGGC CGAGGTCAAT 
GAAGATGTAC AAGAAGATGA AGAAGATAGA GTTGCTGACA CTACTCTCCA GAGCCACATG 
ATAGAATCCA ACTGCCAGAT GAGAACACTG GACAGCGGGA TTGGAACCTT TCCCCTCCCA 
GACTCAGGAA ACCGCTCAGT GGGACGATAC ATATGCCAGC AGGACTCTCC AGAGGACACT 
GATGCCCTCC TGCCTCTCCA GCCAGCCGCT TCCATGGCAT CCTCTGCCAG AGCCCAAACC 
CTTGACCGCG AAGTGCCTTC CTCCACAGAC AGCCAGAGCT CCCCGGAGTC CATGATCATC 
CATTCCATAT CTGACCCCAG CATGATGGCC AGAGAGATGA GAGCCTCACA GAGTCACCTT 
CCCAAGCCTA CCTCCTCAGG AAAACCTAGT TTTGAAAAGC AAAATGGAGC AGAGTCAAAG 
GCTCACACTG GCCCATCATT CGAATATGCT GAGGATGCAG TGGCAAGTGA GCCCCTTTCA 
GGATGGAGGG GTGAAGATGC TACTACTGAG ACCCAGAACA GGTGCCCTGA GGAAGCAGAA 
ATCCTTCACA GACTTGAAAG TGACTCGAGG ACAGTTAATC GTCACCGTGC TCTAGTGAGG 
CAGGAACCGA AACAAGTTGA AGAAACAAAA GAAGATCCTG AGAATAGATT ATCTGAAATT 
TCCCTGGAGT CATTCAATAA ATTTAGCAGC AATACTGTGA TTTTATTAGG AAAAGAGAAG 
TCTCCAAGTG AGGTTGAAGG ACAAAAGGAA GAGAAAGGCA AAAAGACAGA AGTGTCTTCG 
AGTAGCTCCG AACGGCCTGG GGTGGACCGC GTGGAGTCTC TGAGTGACTC TCTGTATGAC 
AGTTTCTCCT CTTGTGCAAG TCAAGCATCC AACGAGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_011247960.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246262.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X7, mRNA.

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CloneID	OMu72731
Clone ID Related Accession (Same CDS sequence)	XM_011247960.2 , XM_011247960.3
Accession Version	XM_011247960.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247960.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAT CAAGCCAACC CAGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GAACAGGTGC 
CCTGAGGAAG CAGAAATCCT TCACAGACTT GAAAGTGACT CGAGGACAGT TAATCGTCAC 
CGTGCTCTAG TGAGGCAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246262.1
CDS	8941..14637
Translation	MMTSSQPREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_011247960.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247960.3

ORF Insert Sequence:

ATGATGACAT CAAGCCAACC CAGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GAACAGGTGC 
CCTGAGGAAG CAGAAATCCT TCACAGACTT GAAAGTGACT CGAGGACAGT TAATCGTCAC 
CGTGCTCTAG TGAGGCAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_030252635.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108495.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X16, mRNA.

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CloneID	OMu72732
Clone ID Related Accession (Same CDS sequence)	XM_030252635.1 , XM_011247962.2
Accession Version	XM_030252635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108495.1
CDS	2649..8102
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQ

Target ORF information:

RefSeq Version	XM_030252635.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252635.1

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_011247963.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246265.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X18, mRNA.

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CloneID	OMu72733
Clone ID Related Accession (Same CDS sequence)	XM_011247963.2 , XM_011247963.3
Accession Version	XM_011247963.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247963.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG AAACGCCACT CACATGTCAT GGCTCTCTCA 
TCTGGTGATC TGCTCTCAGA GGCAGGAGGA AGCCAAAGTC TGGCACTGTC GTGGACAGAT 
ATTGAGCTGC ATGAACACCT CCAGAATGCA AAATCAGGAA CCCCTGCCTT GAAACACTCT 
GGCTCAGGGG TTGTCATCCC TGATCACCTC TGTCCTCGCA ACAGCTGCAG CAGTGGCAGT 
GAGCTATCGC TGTCAAGCAC CTGCAGCGAG TACTCCAGTG GCTCCTCCTA CACGTGGCAA 
GACAGGAAGC TCATTGGGAA AAGGCAATCA TCGCGGATCT GGGATAAAAG ACTAAGTATT 
GATTCTTCGC TCCCAAGTGG CTTCGCCAGC CCTGCAGATG AACTACCTCC GACCCGTATC 
AAAGAAAGCC ACATTCTAGA GGGACTGAGG AAGCTACAGA AGCGGAAAGT GTTCCTTGAA 
TCACCATCAT TGGTGATAAG CAAATGGGGT TATAAAGATT GCATGAACTC GAATGAAGGG 
ATCTATTCGC CAGGAATTAA AACCAGCAGC CTCAAGGAGT GTCCGCCCTG CCAACCGTCT 
GGCATGGGGA TCCCCTGCCA GAATCTCCAT CAGACATTCA TCTATGATGC AAATTCTCAT 
GAGGATGCAC ATGAAGAACT CCCATCCTCA GCCTTGCCAC TAACATCTCC GAGCCAGGGC 
TACAGAATAC AGGGCAGTAA ATTGACACAC AGTGTTTCAG ACAGTCTGTT TAACTGGGAG 
CCACATAGGA AGTACTTTGC AGAAGGTACA GCCTCAGTAT ATACCAGAGA AATGCCTGAA 
AGGCTAGCTC GTTGTGCTAG CCACTGCCCC TTGGAGAGGA AGCTATGTCC CAGTATACAC 
CTGCCCTGGG TGCAAAGAGA CAGGGACCCA CACAGCCAAG TTCATGGCCA TGTGCCTCTG 
AACCTGCAGC TCTCAGATAC CGATGACAAT GAGACCCTAA ACGAGCTACA CATAGAGAGC 
AGTGATGAGA GAAGCCCTTC AGACCTGTCC TTGACTGGGG ACACCGACAA GTCCATGGAG 
AACCTAGAGG TGGGATTCAG AAAGTCTGAA GGTGGCTCTC GGGACATGGG AGAAGAAGCA 
TGTCCCACCC ACTTAGAGAC TAGCCCGAAG GCATTCAGTT TCATCAAGCA ACAAAGGGTT 
GTCAAGAGGA CCTCTTCGGA AGAATGTGTC ACGGTCATAT TTGATGCAGA AAATGGAGAG 
CCAGTGGAAT TCAGCTCTCA CCAGGCTGGA GTCGTCACTG TCACCAGGGA TGAAATTTCC 
ATCAGCTCAG CCCTTGCTGG GCCCTCAGAG GAACTCACTG AACTTTCACC TCAGGAAATT 
ACTCACTTCC AGGCAGGAGC TACTGCAAGA GACTGTCCTT TTCTGAAGAG ACCTGAAGAG 
GAGACTGCGA AAAACATTCC AAAAGATGAA AATCATATTC TCACAGCTCC GAATGACTTC 
TTCAGCTCCC AGACTATAAT AACTCAGAAT TCCCCAAGAC CAAAGCTCGC CAAACCCACA 
CAGGGGGTAG CCTGGCAGAG TAATTCTAGA CCTGTTGGAA ACATGGGTGT CTACACAAAG 
CAAGGTCTGA CAAAAATCCC TTCCAGAGGC AAGTCTTCAC CTCAGAAATC CAAACTTGTG 
GAGTCTATGC CCACCATGCT GGTCCCCTCC AGTGCCCTAG TGACTCCTGA AAAGACACCA 
GCATCCCCTA TGGGGAAACT CTCACAATGT AGAAAGGCAG AGAGCCCTGG TTCTCTCTGT 
GATGTACAGC CAGAATCACA CATTCCAAAA CACCCTGCCC AGGTTCCTCA TATCCCCAAA 
ATTTCCAATA AGAAGAACTG GGTTCAGTGC CCCAGGAGTC AGACTTCAGG GATCCAGTCA 
CGGCATCTCA TGGGGAGAAG TGACAGTAGT GAAATGAGGT CCAATGGCTG TGTCTCTGCG 
CCACAGACAA GAATGAGGTC TCCCTCCCCT CCAACCCCTC CAGGGAGATC AGTGTCACTC 
CTGGTTAGAC CCAGTTATGA TTACCCACCA CTACCATCAC CTGCCAAACC TGAAACTCAG 
GGCCCTGGAG ACGCTGTAAG TCCAGGGTTG AAATCCCCGC TGTTGAAAGG ATCCTCTGCT 
CCTCTTGTCT CTTCCAGTCC AGTGATGTCA GAAATACAGA AGAAACCATC TGTGGCCTCC 
AGAAAGTCTG CCTTCCCTCC AGCTCTTCCC TCAACCCAAG CGGTAGTGCA CACCAGATAC 
CCTGAACACA TAGCCTCCAG TCCATTTGCT GTGACGACTT CAGGGCCCCC AAAAGTCTCT 
CCAAAAAGAG GCATCCCTAA ACCTCCCCCT CACCATGCAC TAGGAACTGC CCACATGGAC 
ACAGACTTAC AGACACCCAA GACTTGTCCT TCAAGCTGTG AACTGCTAGA GGTGACATCT 
TGTAAAAGCC TGTCTCCAGG GAGAAAAAGA CAGTTGAGTG ACAGCACTGT GATGCCCCAC 
AGGCCTTCCT TCTCAGGAGT GAATGAGTCC TTGACACCTC AGGTTAACAG TCCATCCTCC 
TCTTCCTCAT CCTGTAGAAA CCAAGGGTCC TCACAAGGTG GCCAGAACTC TCCCGAGAAA 
GGACCTAAAA CTCGCCTCCC AGTAGGACTC AAAGTTCTCA TGAAGTCTCC TCAGTTGCTC 
AAGAAAACTT CCTTGGTACC AGGGAAACAA GAAAAGGATA GTTTAAATGA AGCATCTCGA 
AGCTGTGTAG CAGCAAACAC GTCTAATCCT GAGGACTCCA AAGACCCTGC AAGTCTGGAG 
ACCTCAAGAG ATGAGAGAAC CACAGCCTTA CTAGGTGTGA GAGGTCCCGA CAGTCTAGCT 
AGAGGACTTG CCTTAGACTC AACAATGCCA GAATCGTTAG AATGTAGCAT CTCCAGAGAT 
GATGGAAGAG ATGGGGCAGA CAGCAGAGCC ATAAAAAGAT CCTTCTCCTC CAGCAAGCCA 
CACCTAAAGC CTGCTCTAGG CATGAATGGA GCCAAAGCTC GGAGTCAGAG CTTCAGCATC 
CACTTGGGAG AGAAGCCTTC CACACCCCCA ACAGAAGGGC CAGGCAAGAT TCGAACTCAG 
ATTATCACCA ACACGGCTGA AAGAGGCAAC TCCCTCACCA GACAGAATAT CTCAATGGAA 
GGGTCCCCAA GCAAGACCCC CTCTGTCTCA ACATCTGATA ATCTGCCCAA TGCTGGAAGG 
CCCCTGGGAA ACCCCTTTTC CAGGCAGAGT TCTCTCTGTA GCACCGGAAG TTCTAGCAGC 
CAGCACGGAA GCCCAAGCAA GTTACCCTTG AGGATGCTCA CAAGGCCGGA AGGACTCCTC 
ACTCCTGGAG TGGAAGACCA ACGGCTCTCT ACTCAGGAAG AGTGTCCTGA AAGAGGTGTA 
CATGAGCAAT CTAGCAGTGA CCACCATAGG CATCCATCTC CTCTTTCAGA CTGCCCTCAG 
TTCCTGCACG TTCCAGGGAG GATGCAGCGC CCAAGCAATT TGGAAACAAG TGGGATATCT 
AAGCTAGAAA CTTCTGGAAG GCATCCAGAT GCTTCTTCAA CTGGGACTAG TGTCGTGAGC 
CCAGAAGCCC CCCTCTCACC CTCCATCGAA GAAAAGGTCA TGTTGTGCAT TCAGGAGAAT 
GTGGAAAAGG GCCAAGTGCA GACAAAGTCT GCCTCTGTGG AAGTGAAGCC CAGGGCAGGG 
CCTTCGTTCA CCAGCTGGTT TGGTTTTCGA AAGAGCAGGC TTCCAGCTCT CAGTAGCAGG 
AGAATGGAAG CTTCCAAGAG TAAAGTGGAA AAGAAAGACA CAAAACTGAA ATCTGAGAGG 
AAAAAAGAAA AGAAGAAAGC TGAAGTACAA TCAAAGATAG AAAACGAACT GAGTAGGGGG 
ACCAAGAAGG CAGATGGTCA GAGTCCAGAC GATGGTCTGC AAAGCTCAGA GAGTCTGAAA 
GTATCCCAAG ACATTTATAA CCAAATGAAG TTGGAGCCAA GGAATAGACC CAGCCCTGCT 
GTGTGCCCAA CAAAAGATGC CTTCATGACA GAACTTCTAA ACAGAGTTGA TAAGAGAGCC 
TCGGTACAGA GAGAGAGTGG ATCAAAGGGT GTTTCCTGCA GGAGCGTGTT AGAGGGCACC 
TCCCAGGGCT CCTGTTTCGC CAGCGGCTCT GTGAGCACTC AAGGAAGTCA AAAGAAAAAC 
ATCAAAACCA AAGTCGATAT GGAAAAACCA AGAGAATCCT TGGGGGCCGA GGTCAATGAA 
GATGTACAAG AAGATGAAGA AGATAGAGTT GCTGACACTA CTCTCCAGAG CCACATGATA 
GAATCCAACT GCCAGATGAG AACACTGGAC AGCGGGATTG GAACCTTTCC CCTCCCAGAC 
TCAGGAAACC GCTCAGTGGG ACGATACATA TGCCAGCAGG ACTCTCCAGA GGACACTGAT 
GCCCTCCTGC CTCTCCAGCC AGCCGCTTCC ATGGCATCCT CTGCCAGAGC CCAAACCCTT 
GACCGCGAAG TGCCTTCCTC CACAGACAGC CAGAGCTCCC CGGAGTCCAT GATCATCCAT 
TCCATATCTG ACCCCAGCAT GATGGCCAGA GAGATGAGAG CCTCACAGAG TCACCTTCCC 
AAGCCTACCT CCTCAGGAAA ACCTAGTTTT GAAAAGCAAA ATGGAGCAGA GTCAAAGGCT 
CACACTGGCC CATCATTCGA ATATGCTGAG GATGCAGTGG CAAGTGAGCC CCTTTCAGGA 
TGGAGGGGTG AAGATGCTAC TACTGAGACC CAGAACAGGT GCCCTGAGGA AGCAGAAATC 
CTTCACAGAC TTGAAAGTGA CTCGAGGACA GTTAATCGTC ACCGTGCTCT AGTGAGGCAG 
GAACCGAAAC AAGTTGAAGA AACAAAAGAA GATCCTGAGA ATAGATTATC TGAAATTTCC 
CTGGAGTCAT TCAATAAATT TAGCAGCAAT ACTGTGATTT TATTAGGAAA AGAGAAGTCT 
CCAAGTGAGG TTGAAGGACA AAAGGAAGAG AAAGGCAAAA AGACAGAAGT GTCTTCGAGT 
AGCTCCGAAC GGCCTGGGGT GGACCGCGTG GAGTCTCTGA GTGACTCTCT GTATGACAGT 
TTCTCCTCTT GTGCAAGTCA AGCATCCAAC GAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246265.1
CDS	2651..8569
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQKRHSHVMALSSGDLLSEAGGSQSLALSWTDIELHEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_011247963.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247963.3

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG AAACGCCACT CACATGTCAT GGCTCTCTCA 
TCTGGTGATC TGCTCTCAGA GGCAGGAGGA AGCCAAAGTC TGGCACTGTC GTGGACAGAT 
ATTGAGCTGC ATGAACACCT CCAGAATGCA AAATCAGGAA CCCCTGCCTT GAAACACTCT 
GGCTCAGGGG TTGTCATCCC TGATCACCTC TGTCCTCGCA ACAGCTGCAG CAGTGGCAGT 
GAGCTATCGC TGTCAAGCAC CTGCAGCGAG TACTCCAGTG GCTCCTCCTA CACGTGGCAA 
GACAGGAAGC TCATTGGGAA AAGGCAATCA TCGCGGATCT GGGATAAAAG ACTAAGTATT 
GATTCTTCGC TCCCAAGTGG CTTCGCCAGC CCTGCAGATG AACTACCTCC GACCCGTATC 
AAAGAAAGCC ACATTCTAGA GGGACTGAGG AAGCTACAGA AGCGGAAAGT GTTCCTTGAA 
TCACCATCAT TGGTGATAAG CAAATGGGGT TATAAAGATT GCATGAACTC GAATGAAGGG 
ATCTATTCGC CAGGAATTAA AACCAGCAGC CTCAAGGAGT GTCCGCCCTG CCAACCGTCT 
GGCATGGGGA TCCCCTGCCA GAATCTCCAT CAGACATTCA TCTATGATGC AAATTCTCAT 
GAGGATGCAC ATGAAGAACT CCCATCCTCA GCCTTGCCAC TAACATCTCC GAGCCAGGGC 
TACAGAATAC AGGGCAGTAA ATTGACACAC AGTGTTTCAG ACAGTCTGTT TAACTGGGAG 
CCACATAGGA AGTACTTTGC AGAAGGTACA GCCTCAGTAT ATACCAGAGA AATGCCTGAA 
AGGCTAGCTC GTTGTGCTAG CCACTGCCCC TTGGAGAGGA AGCTATGTCC CAGTATACAC 
CTGCCCTGGG TGCAAAGAGA CAGGGACCCA CACAGCCAAG TTCATGGCCA TGTGCCTCTG 
AACCTGCAGC TCTCAGATAC CGATGACAAT GAGACCCTAA ACGAGCTACA CATAGAGAGC 
AGTGATGAGA GAAGCCCTTC AGACCTGTCC TTGACTGGGG ACACCGACAA GTCCATGGAG 
AACCTAGAGG TGGGATTCAG AAAGTCTGAA GGTGGCTCTC GGGACATGGG AGAAGAAGCA 
TGTCCCACCC ACTTAGAGAC TAGCCCGAAG GCATTCAGTT TCATCAAGCA ACAAAGGGTT 
GTCAAGAGGA CCTCTTCGGA AGAATGTGTC ACGGTCATAT TTGATGCAGA AAATGGAGAG 
CCAGTGGAAT TCAGCTCTCA CCAGGCTGGA GTCGTCACTG TCACCAGGGA TGAAATTTCC 
ATCAGCTCAG CCCTTGCTGG GCCCTCAGAG GAACTCACTG AACTTTCACC TCAGGAAATT 
ACTCACTTCC AGGCAGGAGC TACTGCAAGA GACTGTCCTT TTCTGAAGAG ACCTGAAGAG 
GAGACTGCGA AAAACATTCC AAAAGATGAA AATCATATTC TCACAGCTCC GAATGACTTC 
TTCAGCTCCC AGACTATAAT AACTCAGAAT TCCCCAAGAC CAAAGCTCGC CAAACCCACA 
CAGGGGGTAG CCTGGCAGAG TAATTCTAGA CCTGTTGGAA ACATGGGTGT CTACACAAAG 
CAAGGTCTGA CAAAAATCCC TTCCAGAGGC AAGTCTTCAC CTCAGAAATC CAAACTTGTG 
GAGTCTATGC CCACCATGCT GGTCCCCTCC AGTGCCCTAG TGACTCCTGA AAAGACACCA 
GCATCCCCTA TGGGGAAACT CTCACAATGT AGAAAGGCAG AGAGCCCTGG TTCTCTCTGT 
GATGTACAGC CAGAATCACA CATTCCAAAA CACCCTGCCC AGGTTCCTCA TATCCCCAAA 
ATTTCCAATA AGAAGAACTG GGTTCAGTGC CCCAGGAGTC AGACTTCAGG GATCCAGTCA 
CGGCATCTCA TGGGGAGAAG TGACAGTAGT GAAATGAGGT CCAATGGCTG TGTCTCTGCG 
CCACAGACAA GAATGAGGTC TCCCTCCCCT CCAACCCCTC CAGGGAGATC AGTGTCACTC 
CTGGTTAGAC CCAGTTATGA TTACCCACCA CTACCATCAC CTGCCAAACC TGAAACTCAG 
GGCCCTGGAG ACGCTGTAAG TCCAGGGTTG AAATCCCCGC TGTTGAAAGG ATCCTCTGCT 
CCTCTTGTCT CTTCCAGTCC AGTGATGTCA GAAATACAGA AGAAACCATC TGTGGCCTCC 
AGAAAGTCTG CCTTCCCTCC AGCTCTTCCC TCAACCCAAG CGGTAGTGCA CACCAGATAC 
CCTGAACACA TAGCCTCCAG TCCATTTGCT GTGACGACTT CAGGGCCCCC AAAAGTCTCT 
CCAAAAAGAG GCATCCCTAA ACCTCCCCCT CACCATGCAC TAGGAACTGC CCACATGGAC 
ACAGACTTAC AGACACCCAA GACTTGTCCT TCAAGCTGTG AACTGCTAGA GGTGACATCT 
TGTAAAAGCC TGTCTCCAGG GAGAAAAAGA CAGTTGAGTG ACAGCACTGT GATGCCCCAC 
AGGCCTTCCT TCTCAGGAGT GAATGAGTCC TTGACACCTC AGGTTAACAG TCCATCCTCC 
TCTTCCTCAT CCTGTAGAAA CCAAGGGTCC TCACAAGGTG GCCAGAACTC TCCCGAGAAA 
GGACCTAAAA CTCGCCTCCC AGTAGGACTC AAAGTTCTCA TGAAGTCTCC TCAGTTGCTC 
AAGAAAACTT CCTTGGTACC AGGGAAACAA GAAAAGGATA GTTTAAATGA AGCATCTCGA 
AGCTGTGTAG CAGCAAACAC GTCTAATCCT GAGGACTCCA AAGACCCTGC AAGTCTGGAG 
ACCTCAAGAG ATGAGAGAAC CACAGCCTTA CTAGGTGTGA GAGGTCCCGA CAGTCTAGCT 
AGAGGACTTG CCTTAGACTC AACAATGCCA GAATCGTTAG AATGTAGCAT CTCCAGAGAT 
GATGGAAGAG ATGGGGCAGA CAGCAGAGCC ATAAAAAGAT CCTTCTCCTC CAGCAAGCCA 
CACCTAAAGC CTGCTCTAGG CATGAATGGA GCCAAAGCTC GGAGTCAGAG CTTCAGCATC 
CACTTGGGAG AGAAGCCTTC CACACCCCCA ACAGAAGGGC CAGGCAAGAT TCGAACTCAG 
ATTATCACCA ACACGGCTGA AAGAGGCAAC TCCCTCACCA GACAGAATAT CTCAATGGAA 
GGGTCCCCAA GCAAGACCCC CTCTGTCTCA ACATCTGATA ATCTGCCCAA TGCTGGAAGG 
CCCCTGGGAA ACCCCTTTTC CAGGCAGAGT TCTCTCTGTA GCACCGGAAG TTCTAGCAGC 
CAGCACGGAA GCCCAAGCAA GTTACCCTTG AGGATGCTCA CAAGGCCGGA AGGACTCCTC 
ACTCCTGGAG TGGAAGACCA ACGGCTCTCT ACTCAGGAAG AGTGTCCTGA AAGAGGTGTA 
CATGAGCAAT CTAGCAGTGA CCACCATAGG CATCCATCTC CTCTTTCAGA CTGCCCTCAG 
TTCCTGCACG TTCCAGGGAG GATGCAGCGC CCAAGCAATT TGGAAACAAG TGGGATATCT 
AAGCTAGAAA CTTCTGGAAG GCATCCAGAT GCTTCTTCAA CTGGGACTAG TGTCGTGAGC 
CCAGAAGCCC CCCTCTCACC CTCCATCGAA GAAAAGGTCA TGTTGTGCAT TCAGGAGAAT 
GTGGAAAAGG GCCAAGTGCA GACAAAGTCT GCCTCTGTGG AAGTGAAGCC CAGGGCAGGG 
CCTTCGTTCA CCAGCTGGTT TGGTTTTCGA AAGAGCAGGC TTCCAGCTCT CAGTAGCAGG 
AGAATGGAAG CTTCCAAGAG TAAAGTGGAA AAGAAAGACA CAAAACTGAA ATCTGAGAGG 
AAAAAAGAAA AGAAGAAAGC TGAAGTACAA TCAAAGATAG AAAACGAACT GAGTAGGGGG 
ACCAAGAAGG CAGATGGTCA GAGTCCAGAC GATGGTCTGC AAAGCTCAGA GAGTCTGAAA 
GTATCCCAAG ACATTTATAA CCAAATGAAG TTGGAGCCAA GGAATAGACC CAGCCCTGCT 
GTGTGCCCAA CAAAAGATGC CTTCATGACA GAACTTCTAA ACAGAGTTGA TAAGAGAGCC 
TCGGTACAGA GAGAGAGTGG ATCAAAGGGT GTTTCCTGCA GGAGCGTGTT AGAGGGCACC 
TCCCAGGGCT CCTGTTTCGC CAGCGGCTCT GTGAGCACTC AAGGAAGTCA AAAGAAAAAC 
ATCAAAACCA AAGTCGATAT GGAAAAACCA AGAGAATCCT TGGGGGCCGA GGTCAATGAA 
GATGTACAAG AAGATGAAGA AGATAGAGTT GCTGACACTA CTCTCCAGAG CCACATGATA 
GAATCCAACT GCCAGATGAG AACACTGGAC AGCGGGATTG GAACCTTTCC CCTCCCAGAC 
TCAGGAAACC GCTCAGTGGG ACGATACATA TGCCAGCAGG ACTCTCCAGA GGACACTGAT 
GCCCTCCTGC CTCTCCAGCC AGCCGCTTCC ATGGCATCCT CTGCCAGAGC CCAAACCCTT 
GACCGCGAAG TGCCTTCCTC CACAGACAGC CAGAGCTCCC CGGAGTCCAT GATCATCCAT 
TCCATATCTG ACCCCAGCAT GATGGCCAGA GAGATGAGAG CCTCACAGAG TCACCTTCCC 
AAGCCTACCT CCTCAGGAAA ACCTAGTTTT GAAAAGCAAA ATGGAGCAGA GTCAAAGGCT 
CACACTGGCC CATCATTCGA ATATGCTGAG GATGCAGTGG CAAGTGAGCC CCTTTCAGGA 
TGGAGGGGTG AAGATGCTAC TACTGAGACC CAGAACAGGT GCCCTGAGGA AGCAGAAATC 
CTTCACAGAC TTGAAAGTGA CTCGAGGACA GTTAATCGTC ACCGTGCTCT AGTGAGGCAG 
GAACCGAAAC AAGTTGAAGA AACAAAAGAA GATCCTGAGA ATAGATTATC TGAAATTTCC 
CTGGAGTCAT TCAATAAATT TAGCAGCAAT ACTGTGATTT TATTAGGAAA AGAGAAGTCT 
CCAAGTGAGG TTGAAGGACA AAAGGAAGAG AAAGGCAAAA AGACAGAAGT GTCTTCGAGT 
AGCTCCGAAC GGCCTGGGGT GGACCGCGTG GAGTCTCTGA GTGACTCTCT GTATGACAGT 
TTCTCCTCTT GTGCAAGTCA AGCATCCAAC GAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_030252637.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108497.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X17, mRNA.

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CloneID	OMu85084
Clone ID Related Accession (Same CDS sequence)	XM_030252637.1
Accession Version	XM_030252637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGAGA AGCTGATACA TGAGTTGGAA GAGGAACGGC ACTTGCGGCT ACAGAGTGAG 
GAGCGGCTGC GGGAGGTGAC CCTGGAGTCA GAGCGCAACA GGATCCAGAT GCTCGGCCTG 
CAGCAGCAGT TTTCCAGAAT GGAAGAAACT GTCCGAAGTC TGTTGCAGAG CCAAGGATCT 
CCAGAGCAGA AAAAGGAAGA ACCTGCCAAG ATCACAGCCT ACCAGGAAGG ACTACCTGAT 
GAAGAGAGAA AAGAGCAAGC AGCACTGGGT GATCTCCATG TGGTGGCAGC TGATGAGGAC 
TCAAGAAGCG AGCACAGTAG TGTGGAGGAG GGAAAGGAAG ACAGCAGACT GCTCCTGAAG 
AGACTGAAGG CCCTGGAGGC GGAGAATTCA GCACTGGCAC TGGAAAACGA GAATCAGAGA 
GAACAGTATG AGCGATGTCT TGATGAGGTA GCCAACCAAG TTGTTCGAGC TCTACTCACT 
CAAAAGGATC TGAGGGAGGA GTGTGTAAAG TTGAAAACAA GAGTGTTTGA CTTGGAGCAA 
CAGAACCGAG CACTCAGCCT CCTGTTCCAA CAGCGAGTCA GACCAGCTTC TGATGTGCTT 
CTCCAGCAAT CATCGCGGAT CTGGGATAAA AGACTAAGTA TTGATTCTTC GCTCCCAAGT 
GGCTTCGCCA GCCCTGCAGA TGAACTACCT CCGACCCGTA TCAAAGAAAG CCACATTCTA 
GAGGGACTGA GGAAGCTACA GAAGCGGAAA GTGTTCCTTG AATCACCATC ATTGGTGATA 
AGCAAATGGG GTTATAAAGA TTGCATGAAC TCGAATGAAG GGATCTATTC GCCAGGAATT 
AAAACCAGCA GCCTCAAGGA GTGTCCGCCC TGCCAACCGT CTGGCATGGG GATCCCCTGC 
CAGAATCTCC ATCAGACATT CATCTATGAT GCAAATTCTC ATGAGGATGC ACATGAAGAA 
CTCCCATCCT CAGCCTTGCC ACTAACATCT CCGAGCCAGG GCTACAGAAT ACAGGGCAGT 
AAATTGACAC ACAGTGTTTC AGACAGTCTG TTTAACTGGG AGCCACATAG GAAGTACTTT 
GCAGAAGGTA CAGCCTCAGT ATATACCAGA GAAATGCCTG AAAGGCTAGC TCGTTGTGCT 
AGCCACTGCC CCTTGGAGAG GAAGCTATGT CCCAGTATAC ACCTGCCCTG GGTGCAAAGA 
GACAGGGACC CACACAGCCA AGTTCATGGC CATGTGCCTC TGAACCTGCA GCTCTCAGAT 
ACCGATGACA ATGAGACCCT AAACGAGCTA CACATAGAGA GCAGTGATGA GAGAAGCCCT 
TCAGACCTGT CCTTGACTGG GGACACCGAC AAGTCCATGG AGAACCTAGA GGTGGGATTC 
AGAAAGTCTG AAGGTGGCTC TCGGGACATG GGAGAAGAAG CATGTCCCAC CCACTTAGAG 
ACTAGCCCGA AGGCATTCAG TTTCATCAAG CAACAAAGGG TTGTCAAGAG GACCTCTTCG 
GAAGAATGTG TCACGGTCAT ATTTGATGCA GAAAATGGAG AGCCAGTGGA ATTCAGCTCT 
CACCAGGCTG GAGTCGTCAC TGTCACCAGG GATGAAATTT CCATCAGCTC AGCCCTTGCT 
GGGCCCTCAG AGGAACTCAC TGAACTTTCA CCTCAGGAAA TTACTCACTT CCAGGCAGGA 
GCTACTGCAA GAGACTGTCC TTTTCTGAAG AGACCTGAAG AGGAGACTGC GAAAAACATT 
CCAAAAGATG AAAATCATAT TCTCACAGCT CCGAATGACT TCTTCAGCTC CCAGACTATA 
ATAACTCAGA ATTCCCCAAG ACCAAAGCTC GCCAAACCCA CACAGGGGGT AGCCTGGCAG 
AGTAATTCTA GACCTGTTGG AAACATGGGT GTCTACACAA AGCAAGGTCT GACAAAAATC 
CCTTCCAGAG GCAAGTCTTC ACCTCAGAAA TCCAAACTTG TGGAGTCTAT GCCCACCATG 
CTGGTCCCCT CCAGTGCCCT AGTGACTCCT GAAAAGACAC CAGCATCCCC TATGGGGAAA 
CTCTCACAAT GTAGAAAGGC AGAGAGCCCT GGTTCTCTCT GTGATGTACA GCCAGAATCA 
CACATTCCAA AACACCCTGC CCAGGTTCCT CATATCCCCA AAATTTCCAA TAAGAAGAAC 
TGGGTTCAGT GCCCCAGGAG TCAGACTTCA GGGATCCAGT CACGGCATCT CATGGGGAGA 
AGTGACAGTA GTGAAATGAG GTCCAATGGC TGTGTCTCTG CGCCACAGAC AAGAATGAGG 
TCTCCCTCCC CTCCAACCCC TCCAGGGAGA TCAGTGTCAC TCCTGGTTAG ACCCAGTTAT 
GATTACCCAC CACTACCATC ACCTGCCAAA CCTGAAACTC AGGGCCCTGG AGACGCTGTA 
AGTCCAGGGT TGAAATCCCC GCTGTTGAAA GGATCCTCTG CTCCTCTTGT CTCTTCCAGT 
CCAGTGATGT CAGAAATACA GAAGAAACCA TCTGTGGCCT CCAGAAAGTC TGCCTTCCCT 
CCAGCTCTTC CCTCAACCCA AGCGGTAGTG CACACCAGAT ACCCTGAACA CATAGCCTCC 
AGTCCATTTG CTGTGACGAC TTCAGGGCCC CCAAAAGTCT CTCCAAAAAG AGGCATCCCT 
AAACCTCCCC CTCACCATGC ACTAGGAACT GCCCACATGG ACACAGACTT ACAGACACCC 
AAGACTTGTC CTTCAAGCTG TGAACTGCTA GAGGTGACAT CTTGTAAAAG CCTGTCTCCA 
GGGAGAAAAA GACAGTTGAG TGACAGCACT GTGATGCCCC ACAGGCCTTC CTTCTCAGGA 
GTGAATGAGT CCTTGACACC TCAGGTTAAC AGTCCATCCT CCTCTTCCTC ATCCTGTAGA 
AACCAAGGGT CCTCACAAGG TGGCCAGAAC TCTCCCGAGA AAGGACCTAA AACTCGCCTC 
CCAGTAGGAC TCAAAGTTCT CATGAAGTCT CCTCAGTTGC TCAAGAAAAC TTCCTTGGTA 
CCAGGGAAAC AAGAAAAGGA TAGTTTAAAT GAAGCATCTC GAAGCTGTGT AGCAGCAAAC 
ACGTCTAATC CTGAGGACTC CAAAGACCCT GCAAGTCTGG AGACCTCAAG AGATGAGAGA 
ACCACAGCCT TACTAGGTGT GAGAGGTCCC GACAGTCTAG CTAGAGGACT TGCCTTAGAC 
TCAACAATGC CAGAATCGTT AGAATGTAGC ATCTCCAGAG ATGATGGAAG AGATGGGGCA 
GACAGCAGAG CCATAAAAAG ATCCTTCTCC TCCAGCAAGC CACACCTAAA GCCTGCTCTA 
GGCATGAATG GAGCCAAAGC TCGGAGTCAG AGCTTCAGCA TCCACTTGGG AGAGAAGCCT 
TCCACACCCC CAACAGAAGG GCCAGGCAAG ATTCGAACTC AGATTATCAC CAACACGGCT 
GAAAGAGGCA ACTCCCTCAC CAGACAGAAT ATCTCAATGG AAGGGTCCCC AAGCAAGACC 
CCCTCTGTCT CAACATCTGA TAATCTGCCC AATGCTGGAA GGCCCCTGGG AAACCCCTTT 
TCCAGGCAGA GTTCTCTCTG TAGCACCGGA AGTTCTAGCA GCCAGCACGG AAGCCCAAGC 
AAGTTACCCT TGAGGATGCT CACAAGGCCG GAAGGACTCC TCACTCCTGG AGTGGAAGAC 
CAACGGCTCT CTACTCAGGA AGAGTGTCCT GAAAGAGGTG TACATGAGCA ATCTAGCAGT 
GACCACCATA GGCATCCATC TCCTCTTTCA GACTGCCCTC AGTTCCTGCA CGTTCCAGGG 
AGGATGCAGC GCCCAAGCAA TTTGGAAACA AGTGGGATAT CTAAGCTAGA AACTTCTGGA 
AGGCATCCAG ATGCTTCTTC AACTGGGACT AGTGTCGTGA GCCCAGAAGC CCCCCTCTCA 
CCCTCCATCG AAGAAAAGGT CATGTTGTGC ATTCAGGAGA ATGTGGAAAA GGGCCAAGTG 
CAGACAAAGT CTGCCTCTGT GGAAGTGAAG CCCAGGGCAG GGCCTTCGTT CACCAGCTGG 
TTTGGTTTTC GAAAGAGCAG GCTTCCAGCT CTCAGTAGCA GGAGAATGGA AGCTTCCAAG 
AGTAAAGTGG AAAAGAAAGA CACAAAACTG AAATCTGAGA GGAAAAAAGA AAAGAAGAAA 
GCTGAAGTAC AATCAAAGAT AGAAAACGAA CTGAGTAGGG GGACCAAGAA GGCAGATGGT 
CAGAGTCCAG ACGATGGTCT GCAAAGCTCA GAGAGTCTGA AAGTATCCCA AGACATTTAT 
AACCAAATGA AGTTGGAGCC AAGGAATAGA CCCAGCCCTG CTGTGTGCCC AACAAAAGAT 
GCCTTCATGA CAGAACTTCT AAACAGAGTT GATAAGAGAG CCTCGGTACA GAGAGAGAGT 
GGATCAAAGG GTGTTTCCTG CAGGAGCGTG TTAGAGGGCA CCTCCCAGGG CTCCTGTTTC 
GCCAGCGGCT CTGTGAGCAC TCAAGGAAGT CAAAAGAAAA ACATCAAAAC CAAAGTCGAT 
ATGGAAAAAC CAAGAGAATC CTTGGGGGCC GAGGTCAATG AAGATGTACA AGAAGATGAA 
GAAGATAGAG TTGCTGACAC TACTCTCCAG AGCCACATGA TAGAATCCAA CTGCCAGATG 
AGAACACTGG ACAGCGGGAT TGGAACCTTT CCCCTCCCAG ACTCAGGAAA CCGCTCAGTG 
GGACGATACA TATGCCAGCA GGACTCTCCA GAGGACACTG ATGCCCTCCT GCCTCTCCAG 
CCAGCCGCTT CCATGGCATC CTCTGCCAGA GCCCAAACCC TTGACCGCGA AGTGCCTTCC 
TCCACAGACA GCCAGAGCTC CCCGGAGTCC ATGATCATCC ATTCCATATC TGACCCCAGC 
ATGATGGCCA GAGAGATGAG AGCCTCACAG AGTCACCTTC CCAAGCCTAC CTCCTCAGGA 
AAACCTAGTT TTGAAAAGCA AAATGGAGCA GAGTCAAAGG CTCACACTGG CCCATCATTC 
GAATATGCTG AGGATGCAGT GGCAAGTGAG CCCCTTTCAG GATGGAGGGG TGAAGATGCT 
ACTACTGAGA CCCAGAACAG GTGCCCTGAG GAAGCAGAAA TCCTTCACAG ACTTGAAAGT 
GACTCGAGGA CAGTTAATCG TCACCGTGCT CTAGTGAGGC AGGAACCGAA ACAAGTTGAA 
GAAACAAAAG AAGATCCTGA GAATAGATTA TCTGAAATTT CCCTGGAGTC ATTCAATAAA 
TTTAGCAGCA ATACTGTGAT TTTATTAGGA AAAGAGAAGT CTCCAAGTGA GGTTGAAGGA 
CAAAAGGAAG AGAAAGGCAA AAAGACAGAA GTGTCTTCGA GTAGCTCCGA ACGGCCTGGG 
GTGGACCGCG TGGAGTCTCT GAGTGACTCT CTGTATGACA GTTTCTCCTC TTGTGCAAGT 
CAAGCATCCA ACGAGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108497.1
CDS	494..5914
Translation	MHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_030252637.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252637.1

ORF Insert Sequence:

ATGCACGAGA AGCTGATACA TGAGTTGGAA GAGGAACGGC ACTTGCGGCT ACAGAGTGAG 
GAGCGGCTGC GGGAGGTGAC CCTGGAGTCA GAGCGCAACA GGATCCAGAT GCTCGGCCTG 
CAGCAGCAGT TTTCCAGAAT GGAAGAAACT GTCCGAAGTC TGTTGCAGAG CCAAGGATCT 
CCAGAGCAGA AAAAGGAAGA ACCTGCCAAG ATCACAGCCT ACCAGGAAGG ACTACCTGAT 
GAAGAGAGAA AAGAGCAAGC AGCACTGGGT GATCTCCATG TGGTGGCAGC TGATGAGGAC 
TCAAGAAGCG AGCACAGTAG TGTGGAGGAG GGAAAGGAAG ACAGCAGACT GCTCCTGAAG 
AGACTGAAGG CCCTGGAGGC GGAGAATTCA GCACTGGCAC TGGAAAACGA GAATCAGAGA 
GAACAGTATG AGCGATGTCT TGATGAGGTA GCCAACCAAG TTGTTCGAGC TCTACTCACT 
CAAAAGGATC TGAGGGAGGA GTGTGTAAAG TTGAAAACAA GAGTGTTTGA CTTGGAGCAA 
CAGAACCGAG CACTCAGCCT CCTGTTCCAA CAGCGAGTCA GACCAGCTTC TGATGTGCTT 
CTCCAGCAAT CATCGCGGAT CTGGGATAAA AGACTAAGTA TTGATTCTTC GCTCCCAAGT 
GGCTTCGCCA GCCCTGCAGA TGAACTACCT CCGACCCGTA TCAAAGAAAG CCACATTCTA 
GAGGGACTGA GGAAGCTACA GAAGCGGAAA GTGTTCCTTG AATCACCATC ATTGGTGATA 
AGCAAATGGG GTTATAAAGA TTGCATGAAC TCGAATGAAG GGATCTATTC GCCAGGAATT 
AAAACCAGCA GCCTCAAGGA GTGTCCGCCC TGCCAACCGT CTGGCATGGG GATCCCCTGC 
CAGAATCTCC ATCAGACATT CATCTATGAT GCAAATTCTC ATGAGGATGC ACATGAAGAA 
CTCCCATCCT CAGCCTTGCC ACTAACATCT CCGAGCCAGG GCTACAGAAT ACAGGGCAGT 
AAATTGACAC ACAGTGTTTC AGACAGTCTG TTTAACTGGG AGCCACATAG GAAGTACTTT 
GCAGAAGGTA CAGCCTCAGT ATATACCAGA GAAATGCCTG AAAGGCTAGC TCGTTGTGCT 
AGCCACTGCC CCTTGGAGAG GAAGCTATGT CCCAGTATAC ACCTGCCCTG GGTGCAAAGA 
GACAGGGACC CACACAGCCA AGTTCATGGC CATGTGCCTC TGAACCTGCA GCTCTCAGAT 
ACCGATGACA ATGAGACCCT AAACGAGCTA CACATAGAGA GCAGTGATGA GAGAAGCCCT 
TCAGACCTGT CCTTGACTGG GGACACCGAC AAGTCCATGG AGAACCTAGA GGTGGGATTC 
AGAAAGTCTG AAGGTGGCTC TCGGGACATG GGAGAAGAAG CATGTCCCAC CCACTTAGAG 
ACTAGCCCGA AGGCATTCAG TTTCATCAAG CAACAAAGGG TTGTCAAGAG GACCTCTTCG 
GAAGAATGTG TCACGGTCAT ATTTGATGCA GAAAATGGAG AGCCAGTGGA ATTCAGCTCT 
CACCAGGCTG GAGTCGTCAC TGTCACCAGG GATGAAATTT CCATCAGCTC AGCCCTTGCT 
GGGCCCTCAG AGGAACTCAC TGAACTTTCA CCTCAGGAAA TTACTCACTT CCAGGCAGGA 
GCTACTGCAA GAGACTGTCC TTTTCTGAAG AGACCTGAAG AGGAGACTGC GAAAAACATT 
CCAAAAGATG AAAATCATAT TCTCACAGCT CCGAATGACT TCTTCAGCTC CCAGACTATA 
ATAACTCAGA ATTCCCCAAG ACCAAAGCTC GCCAAACCCA CACAGGGGGT AGCCTGGCAG 
AGTAATTCTA GACCTGTTGG AAACATGGGT GTCTACACAA AGCAAGGTCT GACAAAAATC 
CCTTCCAGAG GCAAGTCTTC ACCTCAGAAA TCCAAACTTG TGGAGTCTAT GCCCACCATG 
CTGGTCCCCT CCAGTGCCCT AGTGACTCCT GAAAAGACAC CAGCATCCCC TATGGGGAAA 
CTCTCACAAT GTAGAAAGGC AGAGAGCCCT GGTTCTCTCT GTGATGTACA GCCAGAATCA 
CACATTCCAA AACACCCTGC CCAGGTTCCT CATATCCCCA AAATTTCCAA TAAGAAGAAC 
TGGGTTCAGT GCCCCAGGAG TCAGACTTCA GGGATCCAGT CACGGCATCT CATGGGGAGA 
AGTGACAGTA GTGAAATGAG GTCCAATGGC TGTGTCTCTG CGCCACAGAC AAGAATGAGG 
TCTCCCTCCC CTCCAACCCC TCCAGGGAGA TCAGTGTCAC TCCTGGTTAG ACCCAGTTAT 
GATTACCCAC CACTACCATC ACCTGCCAAA CCTGAAACTC AGGGCCCTGG AGACGCTGTA 
AGTCCAGGGT TGAAATCCCC GCTGTTGAAA GGATCCTCTG CTCCTCTTGT CTCTTCCAGT 
CCAGTGATGT CAGAAATACA GAAGAAACCA TCTGTGGCCT CCAGAAAGTC TGCCTTCCCT 
CCAGCTCTTC CCTCAACCCA AGCGGTAGTG CACACCAGAT ACCCTGAACA CATAGCCTCC 
AGTCCATTTG CTGTGACGAC TTCAGGGCCC CCAAAAGTCT CTCCAAAAAG AGGCATCCCT 
AAACCTCCCC CTCACCATGC ACTAGGAACT GCCCACATGG ACACAGACTT ACAGACACCC 
AAGACTTGTC CTTCAAGCTG TGAACTGCTA GAGGTGACAT CTTGTAAAAG CCTGTCTCCA 
GGGAGAAAAA GACAGTTGAG TGACAGCACT GTGATGCCCC ACAGGCCTTC CTTCTCAGGA 
GTGAATGAGT CCTTGACACC TCAGGTTAAC AGTCCATCCT CCTCTTCCTC ATCCTGTAGA 
AACCAAGGGT CCTCACAAGG TGGCCAGAAC TCTCCCGAGA AAGGACCTAA AACTCGCCTC 
CCAGTAGGAC TCAAAGTTCT CATGAAGTCT CCTCAGTTGC TCAAGAAAAC TTCCTTGGTA 
CCAGGGAAAC AAGAAAAGGA TAGTTTAAAT GAAGCATCTC GAAGCTGTGT AGCAGCAAAC 
ACGTCTAATC CTGAGGACTC CAAAGACCCT GCAAGTCTGG AGACCTCAAG AGATGAGAGA 
ACCACAGCCT TACTAGGTGT GAGAGGTCCC GACAGTCTAG CTAGAGGACT TGCCTTAGAC 
TCAACAATGC CAGAATCGTT AGAATGTAGC ATCTCCAGAG ATGATGGAAG AGATGGGGCA 
GACAGCAGAG CCATAAAAAG ATCCTTCTCC TCCAGCAAGC CACACCTAAA GCCTGCTCTA 
GGCATGAATG GAGCCAAAGC TCGGAGTCAG AGCTTCAGCA TCCACTTGGG AGAGAAGCCT 
TCCACACCCC CAACAGAAGG GCCAGGCAAG ATTCGAACTC AGATTATCAC CAACACGGCT 
GAAAGAGGCA ACTCCCTCAC CAGACAGAAT ATCTCAATGG AAGGGTCCCC AAGCAAGACC 
CCCTCTGTCT CAACATCTGA TAATCTGCCC AATGCTGGAA GGCCCCTGGG AAACCCCTTT 
TCCAGGCAGA GTTCTCTCTG TAGCACCGGA AGTTCTAGCA GCCAGCACGG AAGCCCAAGC 
AAGTTACCCT TGAGGATGCT CACAAGGCCG GAAGGACTCC TCACTCCTGG AGTGGAAGAC 
CAACGGCTCT CTACTCAGGA AGAGTGTCCT GAAAGAGGTG TACATGAGCA ATCTAGCAGT 
GACCACCATA GGCATCCATC TCCTCTTTCA GACTGCCCTC AGTTCCTGCA CGTTCCAGGG 
AGGATGCAGC GCCCAAGCAA TTTGGAAACA AGTGGGATAT CTAAGCTAGA AACTTCTGGA 
AGGCATCCAG ATGCTTCTTC AACTGGGACT AGTGTCGTGA GCCCAGAAGC CCCCCTCTCA 
CCCTCCATCG AAGAAAAGGT CATGTTGTGC ATTCAGGAGA ATGTGGAAAA GGGCCAAGTG 
CAGACAAAGT CTGCCTCTGT GGAAGTGAAG CCCAGGGCAG GGCCTTCGTT CACCAGCTGG 
TTTGGTTTTC GAAAGAGCAG GCTTCCAGCT CTCAGTAGCA GGAGAATGGA AGCTTCCAAG 
AGTAAAGTGG AAAAGAAAGA CACAAAACTG AAATCTGAGA GGAAAAAAGA AAAGAAGAAA 
GCTGAAGTAC AATCAAAGAT AGAAAACGAA CTGAGTAGGG GGACCAAGAA GGCAGATGGT 
CAGAGTCCAG ACGATGGTCT GCAAAGCTCA GAGAGTCTGA AAGTATCCCA AGACATTTAT 
AACCAAATGA AGTTGGAGCC AAGGAATAGA CCCAGCCCTG CTGTGTGCCC AACAAAAGAT 
GCCTTCATGA CAGAACTTCT AAACAGAGTT GATAAGAGAG CCTCGGTACA GAGAGAGAGT 
GGATCAAAGG GTGTTTCCTG CAGGAGCGTG TTAGAGGGCA CCTCCCAGGG CTCCTGTTTC 
GCCAGCGGCT CTGTGAGCAC TCAAGGAAGT CAAAAGAAAA ACATCAAAAC CAAAGTCGAT 
ATGGAAAAAC CAAGAGAATC CTTGGGGGCC GAGGTCAATG AAGATGTACA AGAAGATGAA 
GAAGATAGAG TTGCTGACAC TACTCTCCAG AGCCACATGA TAGAATCCAA CTGCCAGATG 
AGAACACTGG ACAGCGGGAT TGGAACCTTT CCCCTCCCAG ACTCAGGAAA CCGCTCAGTG 
GGACGATACA TATGCCAGCA GGACTCTCCA GAGGACACTG ATGCCCTCCT GCCTCTCCAG 
CCAGCCGCTT CCATGGCATC CTCTGCCAGA GCCCAAACCC TTGACCGCGA AGTGCCTTCC 
TCCACAGACA GCCAGAGCTC CCCGGAGTCC ATGATCATCC ATTCCATATC TGACCCCAGC 
ATGATGGCCA GAGAGATGAG AGCCTCACAG AGTCACCTTC CCAAGCCTAC CTCCTCAGGA 
AAACCTAGTT TTGAAAAGCA AAATGGAGCA GAGTCAAAGG CTCACACTGG CCCATCATTC 
GAATATGCTG AGGATGCAGT GGCAAGTGAG CCCCTTTCAG GATGGAGGGG TGAAGATGCT 
ACTACTGAGA CCCAGAACAG GTGCCCTGAG GAAGCAGAAA TCCTTCACAG ACTTGAAAGT 
GACTCGAGGA CAGTTAATCG TCACCGTGCT CTAGTGAGGC AGGAACCGAA ACAAGTTGAA 
GAAACAAAAG AAGATCCTGA GAATAGATTA TCTGAAATTT CCCTGGAGTC ATTCAATAAA 
TTTAGCAGCA ATACTGTGAT TTTATTAGGA AAAGAGAAGT CTCCAAGTGA GGTTGAAGGA 
CAAAAGGAAG AGAAAGGCAA AAAGACAGAA GTGTCTTCGA GTAGCTCCGA ACGGCCTGGG 
GTGGACCGCG TGGAGTCTCT GAGTGACTCT CTGTATGACA GTTTCTCCTC TTGTGCAAGT 
CAAGCATCCA ACGAGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( NM_001081756.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001075225.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant 1, mRNA.

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CloneID	OMu02632
Clone ID Related Accession (Same CDS sequence)	XM_006529330.3 , NM_001081756.1 , XM_006529330.4
Accession Version	NM_001081756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-25
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN700744.1, AK053243.1 and BC066095.1. Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1). ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGAACCGAAA 
CAAGTTGAAG AAACAAAAGA AGATCCTGAG AATAGATTAT CTGAAATTTC CCTGGAGTCA 
TTCAATAAAT TTAGCAGCAA TACTGTGATT TTATTAGGAA AAGAGAAGTC TCCAAGTGAG 
GTTGAAGGAC AAAAGGAAGA GAAAGGCAAA AAGACAGAAG TGTCTTCGAG TAGCTCCGAA 
CGGCCTGGGG TGGACCGCGT GGAGTCTCTG AGTGACTCTC TGTATGACAG TTTCTCCTCT 
TGTGCAAGTC AAGCATCCAA CGAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075225.1
CDS	377..6106
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	NM_001081756.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081756.1

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGAACCGAAA 
CAAGTTGAAG AAACAAAAGA AGATCCTGAG AATAGATTAT CTGAAATTTC CCTGGAGTCA 
TTCAATAAAT TTAGCAGCAA TACTGTGATT TTATTAGGAA AAGAGAAGTC TCCAAGTGAG 
GTTGAAGGAC AAAAGGAAGA GAAAGGCAAA AAGACAGAAG TGTCTTCGAG TAGCTCCGAA 
CGGCCTGGGG TGGACCGCGT GGAGTCTCTG AGTGACTCTC TGTATGACAG TTTCTCCTCT 
TGTGCAAGTC AAGCATCCAA CGAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529330.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529393.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X5, mRNA.

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CloneID	OMu02632
Clone ID Related Accession (Same CDS sequence)	XM_006529330.3 , NM_001081756.1 , XM_006529330.4
Accession Version	XM_006529330.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529330.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGAACCGAAA 
CAAGTTGAAG AAACAAAAGA AGATCCTGAG AATAGATTAT CTGAAATTTC CCTGGAGTCA 
TTCAATAAAT TTAGCAGCAA TACTGTGATT TTATTAGGAA AAGAGAAGTC TCCAAGTGAG 
GTTGAAGGAC AAAAGGAAGA GAAAGGCAAA AAGACAGAAG TGTCTTCGAG TAGCTCCGAA 
CGGCCTGGGG TGGACCGCGT GGAGTCTCTG AGTGACTCTC TGTATGACAG TTTCTCCTCT 
TGTGCAAGTC AAGCATCCAA CGAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529393.1
CDS	845..6574
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_006529330.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529330.3

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGAACCGAAA 
CAAGTTGAAG AAACAAAAGA AGATCCTGAG AATAGATTAT CTGAAATTTC CCTGGAGTCA 
TTCAATAAAT TTAGCAGCAA TACTGTGATT TTATTAGGAA AAGAGAAGTC TCCAAGTGAG 
GTTGAAGGAC AAAAGGAAGA GAAAGGCAAA AAGACAGAAG TGTCTTCGAG TAGCTCCGAA 
CGGCCTGGGG TGGACCGCGT GGAGTCTCTG AGTGACTCTC TGTATGACAG TTTCTCCTCT 
TGTGCAAGTC AAGCATCCAA CGAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529325.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529388.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X1, mRNA.

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CloneID	OMu28056
Clone ID Related Accession (Same CDS sequence)	XM_006529325.3 , XM_006529325.4 , XM_006529326.4 , XM_006529326.3
Accession Version	XM_006529325.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529325.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GAACAGGTGC 
CCTGAGGAAG CAGAAATCCT TCACAGACTT GAAAGTGACT CGAGGACAGT TAATCGTCAC 
CGTGCTCTAG TGAGGCAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529388.1
CDS	847..6663
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_006529325.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529325.3

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GAACAGGTGC 
CCTGAGGAAG CAGAAATCCT TCACAGACTT GAAAGTGACT CGAGGACAGT TAATCGTCAC 
CGTGCTCTAG TGAGGCAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529326.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529389.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X2, mRNA.

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CloneID	OMu28056
Clone ID Related Accession (Same CDS sequence)	XM_006529325.3 , XM_006529325.4 , XM_006529326.4 , XM_006529326.3
Accession Version	XM_006529326.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529326.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GAACAGGTGC 
CCTGAGGAAG CAGAAATCCT TCACAGACTT GAAAGTGACT CGAGGACAGT TAATCGTCAC 
CGTGCTCTAG TGAGGCAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529389.1
CDS	645..6461
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_006529326.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529326.3

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GAACAGGTGC 
CCTGAGGAAG CAGAAATCCT TCACAGACTT GAAAGTGACT CGAGGACAGT TAATCGTCAC 
CGTGCTCTAG TGAGGCAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529329.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529392.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X3, mRNA.

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CloneID	OMu28057
Clone ID Related Accession (Same CDS sequence)	XM_006529329.4 , XM_006529329.3
Accession Version	XM_006529329.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529329.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGTAACCATG 
GGACATGTCA TCTTACAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529392.1
CDS	846..6602
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQVTMGHVILQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_006529329.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529329.3

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGTAACCATG 
GGACATGTCA TCTTACAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529331.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529394.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X8, mRNA.

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CloneID	OMu28058
Clone ID Related Accession (Same CDS sequence)	XM_030252625.1 , XM_006529331.4 , XM_006529331.3
Accession Version	XM_006529331.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529331.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG CAATCATCGC GGATCTGGGA TAAAAGACTA 
AGTATTGATT CTTCGCTCCC AAGTGGCTTC GCCAGCCCTG CAGATGAACT ACCTCCGACC 
CGTATCAAAG AAAGCCACAT TCTAGAGGGA CTGAGGAAGC TACAGAAGCG GAAAGTGTTC 
CTTGAATCAC CATCATTGGT GATAAGCAAA TGGGGTTATA AAGATTGCAT GAACTCGAAT 
GAAGGGATCT ATTCGCCAGG AATTAAAACC AGCAGCCTCA AGGAGTGTCC GCCCTGCCAA 
CCGTCTGGCA TGGGGATCCC CTGCCAGAAT CTCCATCAGA CATTCATCTA TGATGCAAAT 
TCTCATGAGG ATGCACATGA AGAACTCCCA TCCTCAGCCT TGCCACTAAC ATCTCCGAGC 
CAGGGCTACA GAATACAGGG CAGTAAATTG ACACACAGTG TTTCAGACAG TCTGTTTAAC 
TGGGAGCCAC ATAGGAAGTA CTTTGCAGAA GGTACAGCCT CAGTATATAC CAGAGAAATG 
CCTGAAAGGC TAGCTCGTTG TGCTAGCCAC TGCCCCTTGG AGAGGAAGCT ATGTCCCAGT 
ATACACCTGC CCTGGGTGCA AAGAGACAGG GACCCACACA GCCAAGTTCA TGGCCATGTG 
CCTCTGAACC TGCAGCTCTC AGATACCGAT GACAATGAGA CCCTAAACGA GCTACACATA 
GAGAGCAGTG ATGAGAGAAG CCCTTCAGAC CTGTCCTTGA CTGGGGACAC CGACAAGTCC 
ATGGAGAACC TAGAGGTGGG ATTCAGAAAG TCTGAAGGTG GCTCTCGGGA CATGGGAGAA 
GAAGCATGTC CCACCCACTT AGAGACTAGC CCGAAGGCAT TCAGTTTCAT CAAGCAACAA 
AGGGTTGTCA AGAGGACCTC TTCGGAAGAA TGTGTCACGG TCATATTTGA TGCAGAAAAT 
GGAGAGCCAG TGGAATTCAG CTCTCACCAG GCTGGAGTCG TCACTGTCAC CAGGGATGAA 
ATTTCCATCA GCTCAGCCCT TGCTGGGCCC TCAGAGGAAC TCACTGAACT TTCACCTCAG 
GAAATTACTC ACTTCCAGGC AGGAGCTACT GCAAGAGACT GTCCTTTTCT GAAGAGACCT 
GAAGAGGAGA CTGCGAAAAA CATTCCAAAA GATGAAAATC ATATTCTCAC AGCTCCGAAT 
GACTTCTTCA GCTCCCAGAC TATAATAACT CAGAATTCCC CAAGACCAAA GCTCGCCAAA 
CCCACACAGG GGGTAGCCTG GCAGAGTAAT TCTAGACCTG TTGGAAACAT GGGTGTCTAC 
ACAAAGCAAG GTCTGACAAA AATCCCTTCC AGAGGCAAGT CTTCACCTCA GAAATCCAAA 
CTTGTGGAGT CTATGCCCAC CATGCTGGTC CCCTCCAGTG CCCTAGTGAC TCCTGAAAAG 
ACACCAGCAT CCCCTATGGG GAAACTCTCA CAATGTAGAA AGGCAGAGAG CCCTGGTTCT 
CTCTGTGATG TACAGCCAGA ATCACACATT CCAAAACACC CTGCCCAGGT TCCTCATATC 
CCCAAAATTT CCAATAAGAA GAACTGGGTT CAGTGCCCCA GGAGTCAGAC TTCAGGGATC 
CAGTCACGGC ATCTCATGGG GAGAAGTGAC AGTAGTGAAA TGAGGTCCAA TGGCTGTGTC 
TCTGCGCCAC AGACAAGAAT GAGGTCTCCC TCCCCTCCAA CCCCTCCAGG GAGATCAGTG 
TCACTCCTGG TTAGACCCAG TTATGATTAC CCACCACTAC CATCACCTGC CAAACCTGAA 
ACTCAGGGCC CTGGAGACGC TGTAAGTCCA GGGTTGAAAT CCCCGCTGTT GAAAGGATCC 
TCTGCTCCTC TTGTCTCTTC CAGTCCAGTG ATGTCAGAAA TACAGAAGAA ACCATCTGTG 
GCCTCCAGAA AGTCTGCCTT CCCTCCAGCT CTTCCCTCAA CCCAAGCGGT AGTGCACACC 
AGATACCCTG AACACATAGC CTCCAGTCCA TTTGCTGTGA CGACTTCAGG GCCCCCAAAA 
GTCTCTCCAA AAAGAGGCAT CCCTAAACCT CCCCCTCACC ATGCACTAGG AACTGCCCAC 
ATGGACACAG ACTTACAGAC ACCCAAGACT TGTCCTTCAA GCTGTGAACT GCTAGAGGTG 
ACATCTTGTA AAAGCCTGTC TCCAGGGAGA AAAAGACAGT TGAGTGACAG CACTGTGATG 
CCCCACAGGC CTTCCTTCTC AGGAGTGAAT GAGTCCTTGA CACCTCAGGT TAACAGTCCA 
TCCTCCTCTT CCTCATCCTG TAGAAACCAA GGGTCCTCAC AAGGTGGCCA GAACTCTCCC 
GAGAAAGGAC CTAAAACTCG CCTCCCAGTA GGACTCAAAG TTCTCATGAA GTCTCCTCAG 
TTGCTCAAGA AAACTTCCTT GGTACCAGGG AAACAAGAAA AGGATAGTTT AAATGAAGCA 
TCTCGAAGCT GTGTAGCAGC AAACACGTCT AATCCTGAGG ACTCCAAAGA CCCTGCAAGT 
CTGGAGACCT CAAGAGATGA GAGAACCACA GCCTTACTAG GTGTGAGAGG TCCCGACAGT 
CTAGCTAGAG GACTTGCCTT AGACTCAACA ATGCCAGAAT CGTTAGAATG TAGCATCTCC 
AGAGATGATG GAAGAGATGG GGCAGACAGC AGAGCCATAA AAAGATCCTT CTCCTCCAGC 
AAGCCACACC TAAAGCCTGC TCTAGGCATG AATGGAGCCA AAGCTCGGAG TCAGAGCTTC 
AGCATCCACT TGGGAGAGAA GCCTTCCACA CCCCCAACAG AAGGGCCAGG CAAGATTCGA 
ACTCAGATTA TCACCAACAC GGCTGAAAGA GGCAACTCCC TCACCAGACA GAATATCTCA 
ATGGAAGGGT CCCCAAGCAA GACCCCCTCT GTCTCAACAT CTGATAATCT GCCCAATGCT 
GGAAGGCCCC TGGGAAACCC CTTTTCCAGG CAGAGTTCTC TCTGTAGCAC CGGAAGTTCT 
AGCAGCCAGC ACGGAAGCCC AAGCAAGTTA CCCTTGAGGA TGCTCACAAG GCCGGAAGGA 
CTCCTCACTC CTGGAGTGGA AGACCAACGG CTCTCTACTC AGGAAGAGTG TCCTGAAAGA 
GGTGTACATG AGCAATCTAG CAGTGACCAC CATAGGCATC CATCTCCTCT TTCAGACTGC 
CCTCAGTTCC TGCACGTTCC AGGGAGGATG CAGCGCCCAA GCAATTTGGA AACAAGTGGG 
ATATCTAAGC TAGAAACTTC TGGAAGGCAT CCAGATGCTT CTTCAACTGG GACTAGTGTC 
GTGAGCCCAG AAGCCCCCCT CTCACCCTCC ATCGAAGAAA AGGTCATGTT GTGCATTCAG 
GAGAATGTGG AAAAGGGCCA AGTGCAGACA AAGTCTGCCT CTGTGGAAGT GAAGCCCAGG 
GCAGGGCCTT CGTTCACCAG CTGGTTTGGT TTTCGAAAGA GCAGGCTTCC AGCTCTCAGT 
AGCAGGAGAA TGGAAGCTTC CAAGAGTAAA GTGGAAAAGA AAGACACAAA ACTGAAATCT 
GAGAGGAAAA AAGAAAAGAA GAAAGCTGAA GTACAATCAA AGATAGAAAA CGAACTGAGT 
AGGGGGACCA AGAAGGCAGA TGGTCAGAGT CCAGACGATG GTCTGCAAAG CTCAGAGAGT 
CTGAAAGTAT CCCAAGACAT TTATAACCAA ATGAAGTTGG AGCCAAGGAA TAGACCCAGC 
CCTGCTGTGT GCCCAACAAA AGATGCCTTC ATGACAGAAC TTCTAAACAG AGTTGATAAG 
AGAGCCTCGG TACAGAGAGA GAGTGGATCA AAGGGTGTTT CCTGCAGGAG CGTGTTAGAG 
GGCACCTCCC AGGGCTCCTG TTTCGCCAGC GGCTCTGTGA GCACTCAAGG AAGTCAAAAG 
AAAAACATCA AAACCAAAGT CGATATGGAA AAACCAAGAG AATCCTTGGG GGCCGAGGTC 
AATGAAGATG TACAAGAAGA TGAAGAAGAT AGAGTTGCTG ACACTACTCT CCAGAGCCAC 
ATGATAGAAT CCAACTGCCA GATGAGAACA CTGGACAGCG GGATTGGAAC CTTTCCCCTC 
CCAGACTCAG GAAACCGCTC AGTGGGACGA TACATATGCC AGCAGGACTC TCCAGAGGAC 
ACTGATGCCC TCCTGCCTCT CCAGCCAGCC GCTTCCATGG CATCCTCTGC CAGAGCCCAA 
ACCCTTGACC GCGAAGTGCC TTCCTCCACA GACAGCCAGA GCTCCCCGGA GTCCATGATC 
ATCCATTCCA TATCTGACCC CAGCATGATG GCCAGAGAGA TGAGAGCCTC ACAGAGTCAC 
CTTCCCAAGC CTACCTCCTC AGGAAAACCT AGTTTTGAAA AGCAAAATGG AGCAGAGTCA 
AAGGCTCACA CTGGCCCATC ATTCGAATAT GCTGAGGATG CAGTGGCAAG TGAGCCCCTT 
TCAGGATGGA GGGGTGAAGA TGCTACTACT GAGACCCAGA ACAGGTGCCC TGAGGAAGCA 
GAAATCCTTC ACAGACTTGA AAGTGACTCG AGGACAGTTA ATCGTCACCG TGCTCTAGTG 
AGGCAGGAAC CGAAACAAGT TGAAGAAACA AAAGAAGATC CTGAGAATAG ATTATCTGAA 
ATTTCCCTGG AGTCATTCAA TAAATTTAGC AGCAATACTG TGATTTTATT AGGAAAAGAG 
AAGTCTCCAA GTGAGGTTGA AGGACAAAAG GAAGAGAAAG GCAAAAAGAC AGAAGTGTCT 
TCGAGTAGCT CCGAACGGCC TGGGGTGGAC CGCGTGGAGT CTCTGAGTGA CTCTCTGTAT 
GACAGTTTCT CCTCTTGTGC AAGTCAAGCA TCCAACGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529394.1
CDS	848..6472
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_006529331.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529331.3

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG CAATCATCGC GGATCTGGGA TAAAAGACTA 
AGTATTGATT CTTCGCTCCC AAGTGGCTTC GCCAGCCCTG CAGATGAACT ACCTCCGACC 
CGTATCAAAG AAAGCCACAT TCTAGAGGGA CTGAGGAAGC TACAGAAGCG GAAAGTGTTC 
CTTGAATCAC CATCATTGGT GATAAGCAAA TGGGGTTATA AAGATTGCAT GAACTCGAAT 
GAAGGGATCT ATTCGCCAGG AATTAAAACC AGCAGCCTCA AGGAGTGTCC GCCCTGCCAA 
CCGTCTGGCA TGGGGATCCC CTGCCAGAAT CTCCATCAGA CATTCATCTA TGATGCAAAT 
TCTCATGAGG ATGCACATGA AGAACTCCCA TCCTCAGCCT TGCCACTAAC ATCTCCGAGC 
CAGGGCTACA GAATACAGGG CAGTAAATTG ACACACAGTG TTTCAGACAG TCTGTTTAAC 
TGGGAGCCAC ATAGGAAGTA CTTTGCAGAA GGTACAGCCT CAGTATATAC CAGAGAAATG 
CCTGAAAGGC TAGCTCGTTG TGCTAGCCAC TGCCCCTTGG AGAGGAAGCT ATGTCCCAGT 
ATACACCTGC CCTGGGTGCA AAGAGACAGG GACCCACACA GCCAAGTTCA TGGCCATGTG 
CCTCTGAACC TGCAGCTCTC AGATACCGAT GACAATGAGA CCCTAAACGA GCTACACATA 
GAGAGCAGTG ATGAGAGAAG CCCTTCAGAC CTGTCCTTGA CTGGGGACAC CGACAAGTCC 
ATGGAGAACC TAGAGGTGGG ATTCAGAAAG TCTGAAGGTG GCTCTCGGGA CATGGGAGAA 
GAAGCATGTC CCACCCACTT AGAGACTAGC CCGAAGGCAT TCAGTTTCAT CAAGCAACAA 
AGGGTTGTCA AGAGGACCTC TTCGGAAGAA TGTGTCACGG TCATATTTGA TGCAGAAAAT 
GGAGAGCCAG TGGAATTCAG CTCTCACCAG GCTGGAGTCG TCACTGTCAC CAGGGATGAA 
ATTTCCATCA GCTCAGCCCT TGCTGGGCCC TCAGAGGAAC TCACTGAACT TTCACCTCAG 
GAAATTACTC ACTTCCAGGC AGGAGCTACT GCAAGAGACT GTCCTTTTCT GAAGAGACCT 
GAAGAGGAGA CTGCGAAAAA CATTCCAAAA GATGAAAATC ATATTCTCAC AGCTCCGAAT 
GACTTCTTCA GCTCCCAGAC TATAATAACT CAGAATTCCC CAAGACCAAA GCTCGCCAAA 
CCCACACAGG GGGTAGCCTG GCAGAGTAAT TCTAGACCTG TTGGAAACAT GGGTGTCTAC 
ACAAAGCAAG GTCTGACAAA AATCCCTTCC AGAGGCAAGT CTTCACCTCA GAAATCCAAA 
CTTGTGGAGT CTATGCCCAC CATGCTGGTC CCCTCCAGTG CCCTAGTGAC TCCTGAAAAG 
ACACCAGCAT CCCCTATGGG GAAACTCTCA CAATGTAGAA AGGCAGAGAG CCCTGGTTCT 
CTCTGTGATG TACAGCCAGA ATCACACATT CCAAAACACC CTGCCCAGGT TCCTCATATC 
CCCAAAATTT CCAATAAGAA GAACTGGGTT CAGTGCCCCA GGAGTCAGAC TTCAGGGATC 
CAGTCACGGC ATCTCATGGG GAGAAGTGAC AGTAGTGAAA TGAGGTCCAA TGGCTGTGTC 
TCTGCGCCAC AGACAAGAAT GAGGTCTCCC TCCCCTCCAA CCCCTCCAGG GAGATCAGTG 
TCACTCCTGG TTAGACCCAG TTATGATTAC CCACCACTAC CATCACCTGC CAAACCTGAA 
ACTCAGGGCC CTGGAGACGC TGTAAGTCCA GGGTTGAAAT CCCCGCTGTT GAAAGGATCC 
TCTGCTCCTC TTGTCTCTTC CAGTCCAGTG ATGTCAGAAA TACAGAAGAA ACCATCTGTG 
GCCTCCAGAA AGTCTGCCTT CCCTCCAGCT CTTCCCTCAA CCCAAGCGGT AGTGCACACC 
AGATACCCTG AACACATAGC CTCCAGTCCA TTTGCTGTGA CGACTTCAGG GCCCCCAAAA 
GTCTCTCCAA AAAGAGGCAT CCCTAAACCT CCCCCTCACC ATGCACTAGG AACTGCCCAC 
ATGGACACAG ACTTACAGAC ACCCAAGACT TGTCCTTCAA GCTGTGAACT GCTAGAGGTG 
ACATCTTGTA AAAGCCTGTC TCCAGGGAGA AAAAGACAGT TGAGTGACAG CACTGTGATG 
CCCCACAGGC CTTCCTTCTC AGGAGTGAAT GAGTCCTTGA CACCTCAGGT TAACAGTCCA 
TCCTCCTCTT CCTCATCCTG TAGAAACCAA GGGTCCTCAC AAGGTGGCCA GAACTCTCCC 
GAGAAAGGAC CTAAAACTCG CCTCCCAGTA GGACTCAAAG TTCTCATGAA GTCTCCTCAG 
TTGCTCAAGA AAACTTCCTT GGTACCAGGG AAACAAGAAA AGGATAGTTT AAATGAAGCA 
TCTCGAAGCT GTGTAGCAGC AAACACGTCT AATCCTGAGG ACTCCAAAGA CCCTGCAAGT 
CTGGAGACCT CAAGAGATGA GAGAACCACA GCCTTACTAG GTGTGAGAGG TCCCGACAGT 
CTAGCTAGAG GACTTGCCTT AGACTCAACA ATGCCAGAAT CGTTAGAATG TAGCATCTCC 
AGAGATGATG GAAGAGATGG GGCAGACAGC AGAGCCATAA AAAGATCCTT CTCCTCCAGC 
AAGCCACACC TAAAGCCTGC TCTAGGCATG AATGGAGCCA AAGCTCGGAG TCAGAGCTTC 
AGCATCCACT TGGGAGAGAA GCCTTCCACA CCCCCAACAG AAGGGCCAGG CAAGATTCGA 
ACTCAGATTA TCACCAACAC GGCTGAAAGA GGCAACTCCC TCACCAGACA GAATATCTCA 
ATGGAAGGGT CCCCAAGCAA GACCCCCTCT GTCTCAACAT CTGATAATCT GCCCAATGCT 
GGAAGGCCCC TGGGAAACCC CTTTTCCAGG CAGAGTTCTC TCTGTAGCAC CGGAAGTTCT 
AGCAGCCAGC ACGGAAGCCC AAGCAAGTTA CCCTTGAGGA TGCTCACAAG GCCGGAAGGA 
CTCCTCACTC CTGGAGTGGA AGACCAACGG CTCTCTACTC AGGAAGAGTG TCCTGAAAGA 
GGTGTACATG AGCAATCTAG CAGTGACCAC CATAGGCATC CATCTCCTCT TTCAGACTGC 
CCTCAGTTCC TGCACGTTCC AGGGAGGATG CAGCGCCCAA GCAATTTGGA AACAAGTGGG 
ATATCTAAGC TAGAAACTTC TGGAAGGCAT CCAGATGCTT CTTCAACTGG GACTAGTGTC 
GTGAGCCCAG AAGCCCCCCT CTCACCCTCC ATCGAAGAAA AGGTCATGTT GTGCATTCAG 
GAGAATGTGG AAAAGGGCCA AGTGCAGACA AAGTCTGCCT CTGTGGAAGT GAAGCCCAGG 
GCAGGGCCTT CGTTCACCAG CTGGTTTGGT TTTCGAAAGA GCAGGCTTCC AGCTCTCAGT 
AGCAGGAGAA TGGAAGCTTC CAAGAGTAAA GTGGAAAAGA AAGACACAAA ACTGAAATCT 
GAGAGGAAAA AAGAAAAGAA GAAAGCTGAA GTACAATCAA AGATAGAAAA CGAACTGAGT 
AGGGGGACCA AGAAGGCAGA TGGTCAGAGT CCAGACGATG GTCTGCAAAG CTCAGAGAGT 
CTGAAAGTAT CCCAAGACAT TTATAACCAA ATGAAGTTGG AGCCAAGGAA TAGACCCAGC 
CCTGCTGTGT GCCCAACAAA AGATGCCTTC ATGACAGAAC TTCTAAACAG AGTTGATAAG 
AGAGCCTCGG TACAGAGAGA GAGTGGATCA AAGGGTGTTT CCTGCAGGAG CGTGTTAGAG 
GGCACCTCCC AGGGCTCCTG TTTCGCCAGC GGCTCTGTGA GCACTCAAGG AAGTCAAAAG 
AAAAACATCA AAACCAAAGT CGATATGGAA AAACCAAGAG AATCCTTGGG GGCCGAGGTC 
AATGAAGATG TACAAGAAGA TGAAGAAGAT AGAGTTGCTG ACACTACTCT CCAGAGCCAC 
ATGATAGAAT CCAACTGCCA GATGAGAACA CTGGACAGCG GGATTGGAAC CTTTCCCCTC 
CCAGACTCAG GAAACCGCTC AGTGGGACGA TACATATGCC AGCAGGACTC TCCAGAGGAC 
ACTGATGCCC TCCTGCCTCT CCAGCCAGCC GCTTCCATGG CATCCTCTGC CAGAGCCCAA 
ACCCTTGACC GCGAAGTGCC TTCCTCCACA GACAGCCAGA GCTCCCCGGA GTCCATGATC 
ATCCATTCCA TATCTGACCC CAGCATGATG GCCAGAGAGA TGAGAGCCTC ACAGAGTCAC 
CTTCCCAAGC CTACCTCCTC AGGAAAACCT AGTTTTGAAA AGCAAAATGG AGCAGAGTCA 
AAGGCTCACA CTGGCCCATC ATTCGAATAT GCTGAGGATG CAGTGGCAAG TGAGCCCCTT 
TCAGGATGGA GGGGTGAAGA TGCTACTACT GAGACCCAGA ACAGGTGCCC TGAGGAAGCA 
GAAATCCTTC ACAGACTTGA AAGTGACTCG AGGACAGTTA ATCGTCACCG TGCTCTAGTG 
AGGCAGGAAC CGAAACAAGT TGAAGAAACA AAAGAAGATC CTGAGAATAG ATTATCTGAA 
ATTTCCCTGG AGTCATTCAA TAAATTTAGC AGCAATACTG TGATTTTATT AGGAAAAGAG 
AAGTCTCCAA GTGAGGTTGA AGGACAAAAG GAAGAGAAAG GCAAAAAGAC AGAAGTGTCT 
TCGAGTAGCT CCGAACGGCC TGGGGTGGAC CGCGTGGAGT CTCTGAGTGA CTCTCTGTAT 
GACAGTTTCT CCTCTTGTGC AAGTCAAGCA TCCAACGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_017319542.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175031.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X10, mRNA.

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CloneID	OMu28059
Clone ID Related Accession (Same CDS sequence)	XM_017319542.1 , XM_006529332.3 , XM_030252628.1
Accession Version	XM_017319542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGAGA AGCTGATACA TGAGTTGGAA GAGGAACGGC ACTTGCGGCT ACAGAGTGAG 
GAGCGGCTGC GGGAGGTGAC CCTGGAGTCA GAGCGCAACA GGATCCAGAT GCTCGGCCTG 
CAGCAGCAGT TTTCCAGAAT GGAAGAAACT GTCCGAAGTC TGTTGCAGAG CCAAGGATCT 
CCAGAGCAGA AAAAGGAAGA ACCTGCCAAG ATCACAGCCT ACCAGGAAGG ACTACCTGAT 
GAAGAGAGAA AAGAGCAAGC AGCACTGGGT GATCTCCATG TGGTGGCAGC TGATGAGGAC 
TCAAGAAGCG AGCACAGTAG TGTGGAGGAG GGAAAGGAAG ACAGCAGACT GCTCCTGAAG 
AGACTGAAGG CCCTGGAGGC GGAGAATTCA GCACTGGCAC TGGAAAACGA GAATCAGAGA 
GAACAGTATG AGCGATGTCT TGATGAGGTA GCCAACCAAG TTGTTCGAGC TCTACTCACT 
CAAAAGGATC TGAGGGAGGA GTGTGTAAAG TTGAAAACAA GAGTGTTTGA CTTGGAGCAA 
CAGAACCGAG CACTCAGCCT CCTGTTCCAA CAGCGAGTCA GACCAGCTTC TGATGTGCTT 
CTCCAGGAAC ACCTCCAGAA TGCAAAATCA GGAACCCCTG CCTTGAAACA CTCTGGCTCA 
GGGGTTGTCA TCCCTGATCA CCTCTGTCCT CGCAACAGCT GCAGCAGTGG CAGTGAGCTA 
TCGCTGTCAA GCACCTGCAG CGAGTACTCC AGTGGCTCCT CCTACACGTG GCAAGACAGG 
AAGCTCATTG GGAAAAGGCA ATCATCGCGG ATCTGGGATA AAAGACTAAG TATTGATTCT 
TCGCTCCCAA GTGGCTTCGC CAGCCCTGCA GATGAACTAC CTCCGACCCG TATCAAAGAA 
AGCCACATTC TAGAGGGACT GAGGAAGCTA CAGAAGCGGA AAGTGTTCCT TGAATCACCA 
TCATTGGTGA TAAGCAAATG GGGTTATAAA GATTGCATGA ACTCGAATGA AGGGATCTAT 
TCGCCAGGAA TTAAAACCAG CAGCCTCAAG GAGTGTCCGC CCTGCCAACC GTCTGGCATG 
GGGATCCCCT GCCAGAATCT CCATCAGACA TTCATCTATG ATGCAAATTC TCATGAGGAT 
GCACATGAAG AACTCCCATC CTCAGCCTTG CCACTAACAT CTCCGAGCCA GGGCTACAGA 
ATACAGGGCA GTAAATTGAC ACACAGTGTT TCAGACAGTC TGTTTAACTG GGAGCCACAT 
AGGAAGTACT TTGCAGAAGG TACAGCCTCA GTATATACCA GAGAAATGCC TGAAAGGCTA 
GCTCGTTGTG CTAGCCACTG CCCCTTGGAG AGGAAGCTAT GTCCCAGTAT ACACCTGCCC 
TGGGTGCAAA GAGACAGGGA CCCACACAGC CAAGTTCATG GCCATGTGCC TCTGAACCTG 
CAGCTCTCAG ATACCGATGA CAATGAGACC CTAAACGAGC TACACATAGA GAGCAGTGAT 
GAGAGAAGCC CTTCAGACCT GTCCTTGACT GGGGACACCG ACAAGTCCAT GGAGAACCTA 
GAGGTGGGAT TCAGAAAGTC TGAAGGTGGC TCTCGGGACA TGGGAGAAGA AGCATGTCCC 
ACCCACTTAG AGACTAGCCC GAAGGCATTC AGTTTCATCA AGCAACAAAG GGTTGTCAAG 
AGGACCTCTT CGGAAGAATG TGTCACGGTC ATATTTGATG CAGAAAATGG AGAGCCAGTG 
GAATTCAGCT CTCACCAGGC TGGAGTCGTC ACTGTCACCA GGGATGAAAT TTCCATCAGC 
TCAGCCCTTG CTGGGCCCTC AGAGGAACTC ACTGAACTTT CACCTCAGGA AATTACTCAC 
TTCCAGGCAG GAGCTACTGC AAGAGACTGT CCTTTTCTGA AGAGACCTGA AGAGGAGACT 
GCGAAAAACA TTCCAAAAGA TGAAAATCAT ATTCTCACAG CTCCGAATGA CTTCTTCAGC 
TCCCAGACTA TAATAACTCA GAATTCCCCA AGACCAAAGC TCGCCAAACC CACACAGGGG 
GTAGCCTGGC AGAGTAATTC TAGACCTGTT GGAAACATGG GTGTCTACAC AAAGCAAGGT 
CTGACAAAAA TCCCTTCCAG AGGCAAGTCT TCACCTCAGA AATCCAAACT TGTGGAGTCT 
ATGCCCACCA TGCTGGTCCC CTCCAGTGCC CTAGTGACTC CTGAAAAGAC ACCAGCATCC 
CCTATGGGGA AACTCTCACA ATGTAGAAAG GCAGAGAGCC CTGGTTCTCT CTGTGATGTA 
CAGCCAGAAT CACACATTCC AAAACACCCT GCCCAGGTTC CTCATATCCC CAAAATTTCC 
AATAAGAAGA ACTGGGTTCA GTGCCCCAGG AGTCAGACTT CAGGGATCCA GTCACGGCAT 
CTCATGGGGA GAAGTGACAG TAGTGAAATG AGGTCCAATG GCTGTGTCTC TGCGCCACAG 
ACAAGAATGA GGTCTCCCTC CCCTCCAACC CCTCCAGGGA GATCAGTGTC ACTCCTGGTT 
AGACCCAGTT ATGATTACCC ACCACTACCA TCACCTGCCA AACCTGAAAC TCAGGGCCCT 
GGAGACGCTG TAAGTCCAGG GTTGAAATCC CCGCTGTTGA AAGGATCCTC TGCTCCTCTT 
GTCTCTTCCA GTCCAGTGAT GTCAGAAATA CAGAAGAAAC CATCTGTGGC CTCCAGAAAG 
TCTGCCTTCC CTCCAGCTCT TCCCTCAACC CAAGCGGTAG TGCACACCAG ATACCCTGAA 
CACATAGCCT CCAGTCCATT TGCTGTGACG ACTTCAGGGC CCCCAAAAGT CTCTCCAAAA 
AGAGGCATCC CTAAACCTCC CCCTCACCAT GCACTAGGAA CTGCCCACAT GGACACAGAC 
TTACAGACAC CCAAGACTTG TCCTTCAAGC TGTGAACTGC TAGAGGTGAC ATCTTGTAAA 
AGCCTGTCTC CAGGGAGAAA AAGACAGTTG AGTGACAGCA CTGTGATGCC CCACAGGCCT 
TCCTTCTCAG GAGTGAATGA GTCCTTGACA CCTCAGGTTA ACAGTCCATC CTCCTCTTCC 
TCATCCTGTA GAAACCAAGG GTCCTCACAA GGTGGCCAGA ACTCTCCCGA GAAAGGACCT 
AAAACTCGCC TCCCAGTAGG ACTCAAAGTT CTCATGAAGT CTCCTCAGTT GCTCAAGAAA 
ACTTCCTTGG TACCAGGGAA ACAAGAAAAG GATAGTTTAA ATGAAGCATC TCGAAGCTGT 
GTAGCAGCAA ACACGTCTAA TCCTGAGGAC TCCAAAGACC CTGCAAGTCT GGAGACCTCA 
AGAGATGAGA GAACCACAGC CTTACTAGGT GTGAGAGGTC CCGACAGTCT AGCTAGAGGA 
CTTGCCTTAG ACTCAACAAT GCCAGAATCG TTAGAATGTA GCATCTCCAG AGATGATGGA 
AGAGATGGGG CAGACAGCAG AGCCATAAAA AGATCCTTCT CCTCCAGCAA GCCACACCTA 
AAGCCTGCTC TAGGCATGAA TGGAGCCAAA GCTCGGAGTC AGAGCTTCAG CATCCACTTG 
GGAGAGAAGC CTTCCACACC CCCAACAGAA GGGCCAGGCA AGATTCGAAC TCAGATTATC 
ACCAACACGG CTGAAAGAGG CAACTCCCTC ACCAGACAGA ATATCTCAAT GGAAGGGTCC 
CCAAGCAAGA CCCCCTCTGT CTCAACATCT GATAATCTGC CCAATGCTGG AAGGCCCCTG 
GGAAACCCCT TTTCCAGGCA GAGTTCTCTC TGTAGCACCG GAAGTTCTAG CAGCCAGCAC 
GGAAGCCCAA GCAAGTTACC CTTGAGGATG CTCACAAGGC CGGAAGGACT CCTCACTCCT 
GGAGTGGAAG ACCAACGGCT CTCTACTCAG GAAGAGTGTC CTGAAAGAGG TGTACATGAG 
CAATCTAGCA GTGACCACCA TAGGCATCCA TCTCCTCTTT CAGACTGCCC TCAGTTCCTG 
CACGTTCCAG GGAGGATGCA GCGCCCAAGC AATTTGGAAA CAAGTGGGAT ATCTAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCTTCT TCAACTGGGA CTAGTGTCGT GAGCCCAGAA 
GCCCCCCTCT CACCCTCCAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA GAATGTGGAA 
AAGGGCCAAG TGCAGACAAA GTCTGCCTCT GTGGAAGTGA AGCCCAGGGC AGGGCCTTCG 
TTCACCAGCT GGTTTGGTTT TCGAAAGAGC AGGCTTCCAG CTCTCAGTAG CAGGAGAATG 
GAAGCTTCCA AGAGTAAAGT GGAAAAGAAA GACACAAAAC TGAAATCTGA GAGGAAAAAA 
GAAAAGAAGA AAGCTGAAGT ACAATCAAAG ATAGAAAACG AACTGAGTAG GGGGACCAAG 
AAGGCAGATG GTCAGAGTCC AGACGATGGT CTGCAAAGCT CAGAGAGTCT GAAAGTATCC 
CAAGACATTT ATAACCAAAT GAAGTTGGAG CCAAGGAATA GACCCAGCCC TGCTGTGTGC 
CCAACAAAAG ATGCCTTCAT GACAGAACTT CTAAACAGAG TTGATAAGAG AGCCTCGGTA 
CAGAGAGAGA GTGGATCAAA GGGTGTTTCC TGCAGGAGCG TGTTAGAGGG CACCTCCCAG 
GGCTCCTGTT TCGCCAGCGG CTCTGTGAGC ACTCAAGGAA GTCAAAAGAA AAACATCAAA 
ACCAAAGTCG ATATGGAAAA ACCAAGAGAA TCCTTGGGGG CCGAGGTCAA TGAAGATGTA 
CAAGAAGATG AAGAAGATAG AGTTGCTGAC ACTACTCTCC AGAGCCACAT GATAGAATCC 
AACTGCCAGA TGAGAACACT GGACAGCGGG ATTGGAACCT TTCCCCTCCC AGACTCAGGA 
AACCGCTCAG TGGGACGATA CATATGCCAG CAGGACTCTC CAGAGGACAC TGATGCCCTC 
CTGCCTCTCC AGCCAGCCGC TTCCATGGCA TCCTCTGCCA GAGCCCAAAC CCTTGACCGC 
GAAGTGCCTT CCTCCACAGA CAGCCAGAGC TCCCCGGAGT CCATGATCAT CCATTCCATA 
TCTGACCCCA GCATGATGGC CAGAGAGATG AGAGCCTCAC AGAGTCACCT TCCCAAGCCT 
ACCTCCTCAG GAAAACCTAG TTTTGAAAAG CAAAATGGAG CAGAGTCAAA GGCTCACACT 
GGCCCATCAT TCGAATATGC TGAGGATGCA GTGGCAAGTG AGCCCCTTTC AGGATGGAGG 
GGTGAAGATG CTACTACTGA GACCCAGAAC AGGTGCCCTG AGGAAGCAGA AATCCTTCAC 
AGACTTGAAA GTGACTCGAG GACAGTTAAT CGTCACCGTG CTCTAGTGAG GCAGGAACCG 
AAACAAGTTG AAGAAACAAA AGAAGATCCT GAGAATAGAT TATCTGAAAT TTCCCTGGAG 
TCATTCAATA AATTTAGCAG CAATACTGTG ATTTTATTAG GAAAAGAGAA GTCTCCAAGT 
GAGGTTGAAG GACAAAAGGA AGAGAAAGGC AAAAAGACAG AAGTGTCTTC GAGTAGCTCC 
GAACGGCCTG GGGTGGACCG CGTGGAGTCT CTGAGTGACT CTCTGTATGA CAGTTTCTCC 
TCTTGTGCAA GTCAAGCATC CAACGAGGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175031.1
CDS	534..6146
Translation	MHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_017319542.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319542.1

ORF Insert Sequence:

ATGCACGAGA AGCTGATACA TGAGTTGGAA GAGGAACGGC ACTTGCGGCT ACAGAGTGAG 
GAGCGGCTGC GGGAGGTGAC CCTGGAGTCA GAGCGCAACA GGATCCAGAT GCTCGGCCTG 
CAGCAGCAGT TTTCCAGAAT GGAAGAAACT GTCCGAAGTC TGTTGCAGAG CCAAGGATCT 
CCAGAGCAGA AAAAGGAAGA ACCTGCCAAG ATCACAGCCT ACCAGGAAGG ACTACCTGAT 
GAAGAGAGAA AAGAGCAAGC AGCACTGGGT GATCTCCATG TGGTGGCAGC TGATGAGGAC 
TCAAGAAGCG AGCACAGTAG TGTGGAGGAG GGAAAGGAAG ACAGCAGACT GCTCCTGAAG 
AGACTGAAGG CCCTGGAGGC GGAGAATTCA GCACTGGCAC TGGAAAACGA GAATCAGAGA 
GAACAGTATG AGCGATGTCT TGATGAGGTA GCCAACCAAG TTGTTCGAGC TCTACTCACT 
CAAAAGGATC TGAGGGAGGA GTGTGTAAAG TTGAAAACAA GAGTGTTTGA CTTGGAGCAA 
CAGAACCGAG CACTCAGCCT CCTGTTCCAA CAGCGAGTCA GACCAGCTTC TGATGTGCTT 
CTCCAGGAAC ACCTCCAGAA TGCAAAATCA GGAACCCCTG CCTTGAAACA CTCTGGCTCA 
GGGGTTGTCA TCCCTGATCA CCTCTGTCCT CGCAACAGCT GCAGCAGTGG CAGTGAGCTA 
TCGCTGTCAA GCACCTGCAG CGAGTACTCC AGTGGCTCCT CCTACACGTG GCAAGACAGG 
AAGCTCATTG GGAAAAGGCA ATCATCGCGG ATCTGGGATA AAAGACTAAG TATTGATTCT 
TCGCTCCCAA GTGGCTTCGC CAGCCCTGCA GATGAACTAC CTCCGACCCG TATCAAAGAA 
AGCCACATTC TAGAGGGACT GAGGAAGCTA CAGAAGCGGA AAGTGTTCCT TGAATCACCA 
TCATTGGTGA TAAGCAAATG GGGTTATAAA GATTGCATGA ACTCGAATGA AGGGATCTAT 
TCGCCAGGAA TTAAAACCAG CAGCCTCAAG GAGTGTCCGC CCTGCCAACC GTCTGGCATG 
GGGATCCCCT GCCAGAATCT CCATCAGACA TTCATCTATG ATGCAAATTC TCATGAGGAT 
GCACATGAAG AACTCCCATC CTCAGCCTTG CCACTAACAT CTCCGAGCCA GGGCTACAGA 
ATACAGGGCA GTAAATTGAC ACACAGTGTT TCAGACAGTC TGTTTAACTG GGAGCCACAT 
AGGAAGTACT TTGCAGAAGG TACAGCCTCA GTATATACCA GAGAAATGCC TGAAAGGCTA 
GCTCGTTGTG CTAGCCACTG CCCCTTGGAG AGGAAGCTAT GTCCCAGTAT ACACCTGCCC 
TGGGTGCAAA GAGACAGGGA CCCACACAGC CAAGTTCATG GCCATGTGCC TCTGAACCTG 
CAGCTCTCAG ATACCGATGA CAATGAGACC CTAAACGAGC TACACATAGA GAGCAGTGAT 
GAGAGAAGCC CTTCAGACCT GTCCTTGACT GGGGACACCG ACAAGTCCAT GGAGAACCTA 
GAGGTGGGAT TCAGAAAGTC TGAAGGTGGC TCTCGGGACA TGGGAGAAGA AGCATGTCCC 
ACCCACTTAG AGACTAGCCC GAAGGCATTC AGTTTCATCA AGCAACAAAG GGTTGTCAAG 
AGGACCTCTT CGGAAGAATG TGTCACGGTC ATATTTGATG CAGAAAATGG AGAGCCAGTG 
GAATTCAGCT CTCACCAGGC TGGAGTCGTC ACTGTCACCA GGGATGAAAT TTCCATCAGC 
TCAGCCCTTG CTGGGCCCTC AGAGGAACTC ACTGAACTTT CACCTCAGGA AATTACTCAC 
TTCCAGGCAG GAGCTACTGC AAGAGACTGT CCTTTTCTGA AGAGACCTGA AGAGGAGACT 
GCGAAAAACA TTCCAAAAGA TGAAAATCAT ATTCTCACAG CTCCGAATGA CTTCTTCAGC 
TCCCAGACTA TAATAACTCA GAATTCCCCA AGACCAAAGC TCGCCAAACC CACACAGGGG 
GTAGCCTGGC AGAGTAATTC TAGACCTGTT GGAAACATGG GTGTCTACAC AAAGCAAGGT 
CTGACAAAAA TCCCTTCCAG AGGCAAGTCT TCACCTCAGA AATCCAAACT TGTGGAGTCT 
ATGCCCACCA TGCTGGTCCC CTCCAGTGCC CTAGTGACTC CTGAAAAGAC ACCAGCATCC 
CCTATGGGGA AACTCTCACA ATGTAGAAAG GCAGAGAGCC CTGGTTCTCT CTGTGATGTA 
CAGCCAGAAT CACACATTCC AAAACACCCT GCCCAGGTTC CTCATATCCC CAAAATTTCC 
AATAAGAAGA ACTGGGTTCA GTGCCCCAGG AGTCAGACTT CAGGGATCCA GTCACGGCAT 
CTCATGGGGA GAAGTGACAG TAGTGAAATG AGGTCCAATG GCTGTGTCTC TGCGCCACAG 
ACAAGAATGA GGTCTCCCTC CCCTCCAACC CCTCCAGGGA GATCAGTGTC ACTCCTGGTT 
AGACCCAGTT ATGATTACCC ACCACTACCA TCACCTGCCA AACCTGAAAC TCAGGGCCCT 
GGAGACGCTG TAAGTCCAGG GTTGAAATCC CCGCTGTTGA AAGGATCCTC TGCTCCTCTT 
GTCTCTTCCA GTCCAGTGAT GTCAGAAATA CAGAAGAAAC CATCTGTGGC CTCCAGAAAG 
TCTGCCTTCC CTCCAGCTCT TCCCTCAACC CAAGCGGTAG TGCACACCAG ATACCCTGAA 
CACATAGCCT CCAGTCCATT TGCTGTGACG ACTTCAGGGC CCCCAAAAGT CTCTCCAAAA 
AGAGGCATCC CTAAACCTCC CCCTCACCAT GCACTAGGAA CTGCCCACAT GGACACAGAC 
TTACAGACAC CCAAGACTTG TCCTTCAAGC TGTGAACTGC TAGAGGTGAC ATCTTGTAAA 
AGCCTGTCTC CAGGGAGAAA AAGACAGTTG AGTGACAGCA CTGTGATGCC CCACAGGCCT 
TCCTTCTCAG GAGTGAATGA GTCCTTGACA CCTCAGGTTA ACAGTCCATC CTCCTCTTCC 
TCATCCTGTA GAAACCAAGG GTCCTCACAA GGTGGCCAGA ACTCTCCCGA GAAAGGACCT 
AAAACTCGCC TCCCAGTAGG ACTCAAAGTT CTCATGAAGT CTCCTCAGTT GCTCAAGAAA 
ACTTCCTTGG TACCAGGGAA ACAAGAAAAG GATAGTTTAA ATGAAGCATC TCGAAGCTGT 
GTAGCAGCAA ACACGTCTAA TCCTGAGGAC TCCAAAGACC CTGCAAGTCT GGAGACCTCA 
AGAGATGAGA GAACCACAGC CTTACTAGGT GTGAGAGGTC CCGACAGTCT AGCTAGAGGA 
CTTGCCTTAG ACTCAACAAT GCCAGAATCG TTAGAATGTA GCATCTCCAG AGATGATGGA 
AGAGATGGGG CAGACAGCAG AGCCATAAAA AGATCCTTCT CCTCCAGCAA GCCACACCTA 
AAGCCTGCTC TAGGCATGAA TGGAGCCAAA GCTCGGAGTC AGAGCTTCAG CATCCACTTG 
GGAGAGAAGC CTTCCACACC CCCAACAGAA GGGCCAGGCA AGATTCGAAC TCAGATTATC 
ACCAACACGG CTGAAAGAGG CAACTCCCTC ACCAGACAGA ATATCTCAAT GGAAGGGTCC 
CCAAGCAAGA CCCCCTCTGT CTCAACATCT GATAATCTGC CCAATGCTGG AAGGCCCCTG 
GGAAACCCCT TTTCCAGGCA GAGTTCTCTC TGTAGCACCG GAAGTTCTAG CAGCCAGCAC 
GGAAGCCCAA GCAAGTTACC CTTGAGGATG CTCACAAGGC CGGAAGGACT CCTCACTCCT 
GGAGTGGAAG ACCAACGGCT CTCTACTCAG GAAGAGTGTC CTGAAAGAGG TGTACATGAG 
CAATCTAGCA GTGACCACCA TAGGCATCCA TCTCCTCTTT CAGACTGCCC TCAGTTCCTG 
CACGTTCCAG GGAGGATGCA GCGCCCAAGC AATTTGGAAA CAAGTGGGAT ATCTAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCTTCT TCAACTGGGA CTAGTGTCGT GAGCCCAGAA 
GCCCCCCTCT CACCCTCCAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA GAATGTGGAA 
AAGGGCCAAG TGCAGACAAA GTCTGCCTCT GTGGAAGTGA AGCCCAGGGC AGGGCCTTCG 
TTCACCAGCT GGTTTGGTTT TCGAAAGAGC AGGCTTCCAG CTCTCAGTAG CAGGAGAATG 
GAAGCTTCCA AGAGTAAAGT GGAAAAGAAA GACACAAAAC TGAAATCTGA GAGGAAAAAA 
GAAAAGAAGA AAGCTGAAGT ACAATCAAAG ATAGAAAACG AACTGAGTAG GGGGACCAAG 
AAGGCAGATG GTCAGAGTCC AGACGATGGT CTGCAAAGCT CAGAGAGTCT GAAAGTATCC 
CAAGACATTT ATAACCAAAT GAAGTTGGAG CCAAGGAATA GACCCAGCCC TGCTGTGTGC 
CCAACAAAAG ATGCCTTCAT GACAGAACTT CTAAACAGAG TTGATAAGAG AGCCTCGGTA 
CAGAGAGAGA GTGGATCAAA GGGTGTTTCC TGCAGGAGCG TGTTAGAGGG CACCTCCCAG 
GGCTCCTGTT TCGCCAGCGG CTCTGTGAGC ACTCAAGGAA GTCAAAAGAA AAACATCAAA 
ACCAAAGTCG ATATGGAAAA ACCAAGAGAA TCCTTGGGGG CCGAGGTCAA TGAAGATGTA 
CAAGAAGATG AAGAAGATAG AGTTGCTGAC ACTACTCTCC AGAGCCACAT GATAGAATCC 
AACTGCCAGA TGAGAACACT GGACAGCGGG ATTGGAACCT TTCCCCTCCC AGACTCAGGA 
AACCGCTCAG TGGGACGATA CATATGCCAG CAGGACTCTC CAGAGGACAC TGATGCCCTC 
CTGCCTCTCC AGCCAGCCGC TTCCATGGCA TCCTCTGCCA GAGCCCAAAC CCTTGACCGC 
GAAGTGCCTT CCTCCACAGA CAGCCAGAGC TCCCCGGAGT CCATGATCAT CCATTCCATA 
TCTGACCCCA GCATGATGGC CAGAGAGATG AGAGCCTCAC AGAGTCACCT TCCCAAGCCT 
ACCTCCTCAG GAAAACCTAG TTTTGAAAAG CAAAATGGAG CAGAGTCAAA GGCTCACACT 
GGCCCATCAT TCGAATATGC TGAGGATGCA GTGGCAAGTG AGCCCCTTTC AGGATGGAGG 
GGTGAAGATG CTACTACTGA GACCCAGAAC AGGTGCCCTG AGGAAGCAGA AATCCTTCAC 
AGACTTGAAA GTGACTCGAG GACAGTTAAT CGTCACCGTG CTCTAGTGAG GCAGGAACCG 
AAACAAGTTG AAGAAACAAA AGAAGATCCT GAGAATAGAT TATCTGAAAT TTCCCTGGAG 
TCATTCAATA AATTTAGCAG CAATACTGTG ATTTTATTAG GAAAAGAGAA GTCTCCAAGT 
GAGGTTGAAG GACAAAAGGA AGAGAAAGGC AAAAAGACAG AAGTGTCTTC GAGTAGCTCC 
GAACGGCCTG GGGTGGACCG CGTGGAGTCT CTGAGTGACT CTCTGTATGA CAGTTTCTCC 
TCTTGTGCAA GTCAAGCATC CAACGAGGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529332.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529395.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X10, mRNA.

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CloneID	OMu28059
Clone ID Related Accession (Same CDS sequence)	XM_017319542.1 , XM_006529332.3 , XM_030252628.1
Accession Version	XM_006529332.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529332.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGAGA AGCTGATACA TGAGTTGGAA GAGGAACGGC ACTTGCGGCT ACAGAGTGAG 
GAGCGGCTGC GGGAGGTGAC CCTGGAGTCA GAGCGCAACA GGATCCAGAT GCTCGGCCTG 
CAGCAGCAGT TTTCCAGAAT GGAAGAAACT GTCCGAAGTC TGTTGCAGAG CCAAGGATCT 
CCAGAGCAGA AAAAGGAAGA ACCTGCCAAG ATCACAGCCT ACCAGGAAGG ACTACCTGAT 
GAAGAGAGAA AAGAGCAAGC AGCACTGGGT GATCTCCATG TGGTGGCAGC TGATGAGGAC 
TCAAGAAGCG AGCACAGTAG TGTGGAGGAG GGAAAGGAAG ACAGCAGACT GCTCCTGAAG 
AGACTGAAGG CCCTGGAGGC GGAGAATTCA GCACTGGCAC TGGAAAACGA GAATCAGAGA 
GAACAGTATG AGCGATGTCT TGATGAGGTA GCCAACCAAG TTGTTCGAGC TCTACTCACT 
CAAAAGGATC TGAGGGAGGA GTGTGTAAAG TTGAAAACAA GAGTGTTTGA CTTGGAGCAA 
CAGAACCGAG CACTCAGCCT CCTGTTCCAA CAGCGAGTCA GACCAGCTTC TGATGTGCTT 
CTCCAGGAAC ACCTCCAGAA TGCAAAATCA GGAACCCCTG CCTTGAAACA CTCTGGCTCA 
GGGGTTGTCA TCCCTGATCA CCTCTGTCCT CGCAACAGCT GCAGCAGTGG CAGTGAGCTA 
TCGCTGTCAA GCACCTGCAG CGAGTACTCC AGTGGCTCCT CCTACACGTG GCAAGACAGG 
AAGCTCATTG GGAAAAGGCA ATCATCGCGG ATCTGGGATA AAAGACTAAG TATTGATTCT 
TCGCTCCCAA GTGGCTTCGC CAGCCCTGCA GATGAACTAC CTCCGACCCG TATCAAAGAA 
AGCCACATTC TAGAGGGACT GAGGAAGCTA CAGAAGCGGA AAGTGTTCCT TGAATCACCA 
TCATTGGTGA TAAGCAAATG GGGTTATAAA GATTGCATGA ACTCGAATGA AGGGATCTAT 
TCGCCAGGAA TTAAAACCAG CAGCCTCAAG GAGTGTCCGC CCTGCCAACC GTCTGGCATG 
GGGATCCCCT GCCAGAATCT CCATCAGACA TTCATCTATG ATGCAAATTC TCATGAGGAT 
GCACATGAAG AACTCCCATC CTCAGCCTTG CCACTAACAT CTCCGAGCCA GGGCTACAGA 
ATACAGGGCA GTAAATTGAC ACACAGTGTT TCAGACAGTC TGTTTAACTG GGAGCCACAT 
AGGAAGTACT TTGCAGAAGG TACAGCCTCA GTATATACCA GAGAAATGCC TGAAAGGCTA 
GCTCGTTGTG CTAGCCACTG CCCCTTGGAG AGGAAGCTAT GTCCCAGTAT ACACCTGCCC 
TGGGTGCAAA GAGACAGGGA CCCACACAGC CAAGTTCATG GCCATGTGCC TCTGAACCTG 
CAGCTCTCAG ATACCGATGA CAATGAGACC CTAAACGAGC TACACATAGA GAGCAGTGAT 
GAGAGAAGCC CTTCAGACCT GTCCTTGACT GGGGACACCG ACAAGTCCAT GGAGAACCTA 
GAGGTGGGAT TCAGAAAGTC TGAAGGTGGC TCTCGGGACA TGGGAGAAGA AGCATGTCCC 
ACCCACTTAG AGACTAGCCC GAAGGCATTC AGTTTCATCA AGCAACAAAG GGTTGTCAAG 
AGGACCTCTT CGGAAGAATG TGTCACGGTC ATATTTGATG CAGAAAATGG AGAGCCAGTG 
GAATTCAGCT CTCACCAGGC TGGAGTCGTC ACTGTCACCA GGGATGAAAT TTCCATCAGC 
TCAGCCCTTG CTGGGCCCTC AGAGGAACTC ACTGAACTTT CACCTCAGGA AATTACTCAC 
TTCCAGGCAG GAGCTACTGC AAGAGACTGT CCTTTTCTGA AGAGACCTGA AGAGGAGACT 
GCGAAAAACA TTCCAAAAGA TGAAAATCAT ATTCTCACAG CTCCGAATGA CTTCTTCAGC 
TCCCAGACTA TAATAACTCA GAATTCCCCA AGACCAAAGC TCGCCAAACC CACACAGGGG 
GTAGCCTGGC AGAGTAATTC TAGACCTGTT GGAAACATGG GTGTCTACAC AAAGCAAGGT 
CTGACAAAAA TCCCTTCCAG AGGCAAGTCT TCACCTCAGA AATCCAAACT TGTGGAGTCT 
ATGCCCACCA TGCTGGTCCC CTCCAGTGCC CTAGTGACTC CTGAAAAGAC ACCAGCATCC 
CCTATGGGGA AACTCTCACA ATGTAGAAAG GCAGAGAGCC CTGGTTCTCT CTGTGATGTA 
CAGCCAGAAT CACACATTCC AAAACACCCT GCCCAGGTTC CTCATATCCC CAAAATTTCC 
AATAAGAAGA ACTGGGTTCA GTGCCCCAGG AGTCAGACTT CAGGGATCCA GTCACGGCAT 
CTCATGGGGA GAAGTGACAG TAGTGAAATG AGGTCCAATG GCTGTGTCTC TGCGCCACAG 
ACAAGAATGA GGTCTCCCTC CCCTCCAACC CCTCCAGGGA GATCAGTGTC ACTCCTGGTT 
AGACCCAGTT ATGATTACCC ACCACTACCA TCACCTGCCA AACCTGAAAC TCAGGGCCCT 
GGAGACGCTG TAAGTCCAGG GTTGAAATCC CCGCTGTTGA AAGGATCCTC TGCTCCTCTT 
GTCTCTTCCA GTCCAGTGAT GTCAGAAATA CAGAAGAAAC CATCTGTGGC CTCCAGAAAG 
TCTGCCTTCC CTCCAGCTCT TCCCTCAACC CAAGCGGTAG TGCACACCAG ATACCCTGAA 
CACATAGCCT CCAGTCCATT TGCTGTGACG ACTTCAGGGC CCCCAAAAGT CTCTCCAAAA 
AGAGGCATCC CTAAACCTCC CCCTCACCAT GCACTAGGAA CTGCCCACAT GGACACAGAC 
TTACAGACAC CCAAGACTTG TCCTTCAAGC TGTGAACTGC TAGAGGTGAC ATCTTGTAAA 
AGCCTGTCTC CAGGGAGAAA AAGACAGTTG AGTGACAGCA CTGTGATGCC CCACAGGCCT 
TCCTTCTCAG GAGTGAATGA GTCCTTGACA CCTCAGGTTA ACAGTCCATC CTCCTCTTCC 
TCATCCTGTA GAAACCAAGG GTCCTCACAA GGTGGCCAGA ACTCTCCCGA GAAAGGACCT 
AAAACTCGCC TCCCAGTAGG ACTCAAAGTT CTCATGAAGT CTCCTCAGTT GCTCAAGAAA 
ACTTCCTTGG TACCAGGGAA ACAAGAAAAG GATAGTTTAA ATGAAGCATC TCGAAGCTGT 
GTAGCAGCAA ACACGTCTAA TCCTGAGGAC TCCAAAGACC CTGCAAGTCT GGAGACCTCA 
AGAGATGAGA GAACCACAGC CTTACTAGGT GTGAGAGGTC CCGACAGTCT AGCTAGAGGA 
CTTGCCTTAG ACTCAACAAT GCCAGAATCG TTAGAATGTA GCATCTCCAG AGATGATGGA 
AGAGATGGGG CAGACAGCAG AGCCATAAAA AGATCCTTCT CCTCCAGCAA GCCACACCTA 
AAGCCTGCTC TAGGCATGAA TGGAGCCAAA GCTCGGAGTC AGAGCTTCAG CATCCACTTG 
GGAGAGAAGC CTTCCACACC CCCAACAGAA GGGCCAGGCA AGATTCGAAC TCAGATTATC 
ACCAACACGG CTGAAAGAGG CAACTCCCTC ACCAGACAGA ATATCTCAAT GGAAGGGTCC 
CCAAGCAAGA CCCCCTCTGT CTCAACATCT GATAATCTGC CCAATGCTGG AAGGCCCCTG 
GGAAACCCCT TTTCCAGGCA GAGTTCTCTC TGTAGCACCG GAAGTTCTAG CAGCCAGCAC 
GGAAGCCCAA GCAAGTTACC CTTGAGGATG CTCACAAGGC CGGAAGGACT CCTCACTCCT 
GGAGTGGAAG ACCAACGGCT CTCTACTCAG GAAGAGTGTC CTGAAAGAGG TGTACATGAG 
CAATCTAGCA GTGACCACCA TAGGCATCCA TCTCCTCTTT CAGACTGCCC TCAGTTCCTG 
CACGTTCCAG GGAGGATGCA GCGCCCAAGC AATTTGGAAA CAAGTGGGAT ATCTAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCTTCT TCAACTGGGA CTAGTGTCGT GAGCCCAGAA 
GCCCCCCTCT CACCCTCCAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA GAATGTGGAA 
AAGGGCCAAG TGCAGACAAA GTCTGCCTCT GTGGAAGTGA AGCCCAGGGC AGGGCCTTCG 
TTCACCAGCT GGTTTGGTTT TCGAAAGAGC AGGCTTCCAG CTCTCAGTAG CAGGAGAATG 
GAAGCTTCCA AGAGTAAAGT GGAAAAGAAA GACACAAAAC TGAAATCTGA GAGGAAAAAA 
GAAAAGAAGA AAGCTGAAGT ACAATCAAAG ATAGAAAACG AACTGAGTAG GGGGACCAAG 
AAGGCAGATG GTCAGAGTCC AGACGATGGT CTGCAAAGCT CAGAGAGTCT GAAAGTATCC 
CAAGACATTT ATAACCAAAT GAAGTTGGAG CCAAGGAATA GACCCAGCCC TGCTGTGTGC 
CCAACAAAAG ATGCCTTCAT GACAGAACTT CTAAACAGAG TTGATAAGAG AGCCTCGGTA 
CAGAGAGAGA GTGGATCAAA GGGTGTTTCC TGCAGGAGCG TGTTAGAGGG CACCTCCCAG 
GGCTCCTGTT TCGCCAGCGG CTCTGTGAGC ACTCAAGGAA GTCAAAAGAA AAACATCAAA 
ACCAAAGTCG ATATGGAAAA ACCAAGAGAA TCCTTGGGGG CCGAGGTCAA TGAAGATGTA 
CAAGAAGATG AAGAAGATAG AGTTGCTGAC ACTACTCTCC AGAGCCACAT GATAGAATCC 
AACTGCCAGA TGAGAACACT GGACAGCGGG ATTGGAACCT TTCCCCTCCC AGACTCAGGA 
AACCGCTCAG TGGGACGATA CATATGCCAG CAGGACTCTC CAGAGGACAC TGATGCCCTC 
CTGCCTCTCC AGCCAGCCGC TTCCATGGCA TCCTCTGCCA GAGCCCAAAC CCTTGACCGC 
GAAGTGCCTT CCTCCACAGA CAGCCAGAGC TCCCCGGAGT CCATGATCAT CCATTCCATA 
TCTGACCCCA GCATGATGGC CAGAGAGATG AGAGCCTCAC AGAGTCACCT TCCCAAGCCT 
ACCTCCTCAG GAAAACCTAG TTTTGAAAAG CAAAATGGAG CAGAGTCAAA GGCTCACACT 
GGCCCATCAT TCGAATATGC TGAGGATGCA GTGGCAAGTG AGCCCCTTTC AGGATGGAGG 
GGTGAAGATG CTACTACTGA GACCCAGAAC AGGTGCCCTG AGGAAGCAGA AATCCTTCAC 
AGACTTGAAA GTGACTCGAG GACAGTTAAT CGTCACCGTG CTCTAGTGAG GCAGGAACCG 
AAACAAGTTG AAGAAACAAA AGAAGATCCT GAGAATAGAT TATCTGAAAT TTCCCTGGAG 
TCATTCAATA AATTTAGCAG CAATACTGTG ATTTTATTAG GAAAAGAGAA GTCTCCAAGT 
GAGGTTGAAG GACAAAAGGA AGAGAAAGGC AAAAAGACAG AAGTGTCTTC GAGTAGCTCC 
GAACGGCCTG GGGTGGACCG CGTGGAGTCT CTGAGTGACT CTCTGTATGA CAGTTTCTCC 
TCTTGTGCAA GTCAAGCATC CAACGAGGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529395.1
CDS	494..6106
Translation	MHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_006529332.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529332.3

ORF Insert Sequence:

ATGCACGAGA AGCTGATACA TGAGTTGGAA GAGGAACGGC ACTTGCGGCT ACAGAGTGAG 
GAGCGGCTGC GGGAGGTGAC CCTGGAGTCA GAGCGCAACA GGATCCAGAT GCTCGGCCTG 
CAGCAGCAGT TTTCCAGAAT GGAAGAAACT GTCCGAAGTC TGTTGCAGAG CCAAGGATCT 
CCAGAGCAGA AAAAGGAAGA ACCTGCCAAG ATCACAGCCT ACCAGGAAGG ACTACCTGAT 
GAAGAGAGAA AAGAGCAAGC AGCACTGGGT GATCTCCATG TGGTGGCAGC TGATGAGGAC 
TCAAGAAGCG AGCACAGTAG TGTGGAGGAG GGAAAGGAAG ACAGCAGACT GCTCCTGAAG 
AGACTGAAGG CCCTGGAGGC GGAGAATTCA GCACTGGCAC TGGAAAACGA GAATCAGAGA 
GAACAGTATG AGCGATGTCT TGATGAGGTA GCCAACCAAG TTGTTCGAGC TCTACTCACT 
CAAAAGGATC TGAGGGAGGA GTGTGTAAAG TTGAAAACAA GAGTGTTTGA CTTGGAGCAA 
CAGAACCGAG CACTCAGCCT CCTGTTCCAA CAGCGAGTCA GACCAGCTTC TGATGTGCTT 
CTCCAGGAAC ACCTCCAGAA TGCAAAATCA GGAACCCCTG CCTTGAAACA CTCTGGCTCA 
GGGGTTGTCA TCCCTGATCA CCTCTGTCCT CGCAACAGCT GCAGCAGTGG CAGTGAGCTA 
TCGCTGTCAA GCACCTGCAG CGAGTACTCC AGTGGCTCCT CCTACACGTG GCAAGACAGG 
AAGCTCATTG GGAAAAGGCA ATCATCGCGG ATCTGGGATA AAAGACTAAG TATTGATTCT 
TCGCTCCCAA GTGGCTTCGC CAGCCCTGCA GATGAACTAC CTCCGACCCG TATCAAAGAA 
AGCCACATTC TAGAGGGACT GAGGAAGCTA CAGAAGCGGA AAGTGTTCCT TGAATCACCA 
TCATTGGTGA TAAGCAAATG GGGTTATAAA GATTGCATGA ACTCGAATGA AGGGATCTAT 
TCGCCAGGAA TTAAAACCAG CAGCCTCAAG GAGTGTCCGC CCTGCCAACC GTCTGGCATG 
GGGATCCCCT GCCAGAATCT CCATCAGACA TTCATCTATG ATGCAAATTC TCATGAGGAT 
GCACATGAAG AACTCCCATC CTCAGCCTTG CCACTAACAT CTCCGAGCCA GGGCTACAGA 
ATACAGGGCA GTAAATTGAC ACACAGTGTT TCAGACAGTC TGTTTAACTG GGAGCCACAT 
AGGAAGTACT TTGCAGAAGG TACAGCCTCA GTATATACCA GAGAAATGCC TGAAAGGCTA 
GCTCGTTGTG CTAGCCACTG CCCCTTGGAG AGGAAGCTAT GTCCCAGTAT ACACCTGCCC 
TGGGTGCAAA GAGACAGGGA CCCACACAGC CAAGTTCATG GCCATGTGCC TCTGAACCTG 
CAGCTCTCAG ATACCGATGA CAATGAGACC CTAAACGAGC TACACATAGA GAGCAGTGAT 
GAGAGAAGCC CTTCAGACCT GTCCTTGACT GGGGACACCG ACAAGTCCAT GGAGAACCTA 
GAGGTGGGAT TCAGAAAGTC TGAAGGTGGC TCTCGGGACA TGGGAGAAGA AGCATGTCCC 
ACCCACTTAG AGACTAGCCC GAAGGCATTC AGTTTCATCA AGCAACAAAG GGTTGTCAAG 
AGGACCTCTT CGGAAGAATG TGTCACGGTC ATATTTGATG CAGAAAATGG AGAGCCAGTG 
GAATTCAGCT CTCACCAGGC TGGAGTCGTC ACTGTCACCA GGGATGAAAT TTCCATCAGC 
TCAGCCCTTG CTGGGCCCTC AGAGGAACTC ACTGAACTTT CACCTCAGGA AATTACTCAC 
TTCCAGGCAG GAGCTACTGC AAGAGACTGT CCTTTTCTGA AGAGACCTGA AGAGGAGACT 
GCGAAAAACA TTCCAAAAGA TGAAAATCAT ATTCTCACAG CTCCGAATGA CTTCTTCAGC 
TCCCAGACTA TAATAACTCA GAATTCCCCA AGACCAAAGC TCGCCAAACC CACACAGGGG 
GTAGCCTGGC AGAGTAATTC TAGACCTGTT GGAAACATGG GTGTCTACAC AAAGCAAGGT 
CTGACAAAAA TCCCTTCCAG AGGCAAGTCT TCACCTCAGA AATCCAAACT TGTGGAGTCT 
ATGCCCACCA TGCTGGTCCC CTCCAGTGCC CTAGTGACTC CTGAAAAGAC ACCAGCATCC 
CCTATGGGGA AACTCTCACA ATGTAGAAAG GCAGAGAGCC CTGGTTCTCT CTGTGATGTA 
CAGCCAGAAT CACACATTCC AAAACACCCT GCCCAGGTTC CTCATATCCC CAAAATTTCC 
AATAAGAAGA ACTGGGTTCA GTGCCCCAGG AGTCAGACTT CAGGGATCCA GTCACGGCAT 
CTCATGGGGA GAAGTGACAG TAGTGAAATG AGGTCCAATG GCTGTGTCTC TGCGCCACAG 
ACAAGAATGA GGTCTCCCTC CCCTCCAACC CCTCCAGGGA GATCAGTGTC ACTCCTGGTT 
AGACCCAGTT ATGATTACCC ACCACTACCA TCACCTGCCA AACCTGAAAC TCAGGGCCCT 
GGAGACGCTG TAAGTCCAGG GTTGAAATCC CCGCTGTTGA AAGGATCCTC TGCTCCTCTT 
GTCTCTTCCA GTCCAGTGAT GTCAGAAATA CAGAAGAAAC CATCTGTGGC CTCCAGAAAG 
TCTGCCTTCC CTCCAGCTCT TCCCTCAACC CAAGCGGTAG TGCACACCAG ATACCCTGAA 
CACATAGCCT CCAGTCCATT TGCTGTGACG ACTTCAGGGC CCCCAAAAGT CTCTCCAAAA 
AGAGGCATCC CTAAACCTCC CCCTCACCAT GCACTAGGAA CTGCCCACAT GGACACAGAC 
TTACAGACAC CCAAGACTTG TCCTTCAAGC TGTGAACTGC TAGAGGTGAC ATCTTGTAAA 
AGCCTGTCTC CAGGGAGAAA AAGACAGTTG AGTGACAGCA CTGTGATGCC CCACAGGCCT 
TCCTTCTCAG GAGTGAATGA GTCCTTGACA CCTCAGGTTA ACAGTCCATC CTCCTCTTCC 
TCATCCTGTA GAAACCAAGG GTCCTCACAA GGTGGCCAGA ACTCTCCCGA GAAAGGACCT 
AAAACTCGCC TCCCAGTAGG ACTCAAAGTT CTCATGAAGT CTCCTCAGTT GCTCAAGAAA 
ACTTCCTTGG TACCAGGGAA ACAAGAAAAG GATAGTTTAA ATGAAGCATC TCGAAGCTGT 
GTAGCAGCAA ACACGTCTAA TCCTGAGGAC TCCAAAGACC CTGCAAGTCT GGAGACCTCA 
AGAGATGAGA GAACCACAGC CTTACTAGGT GTGAGAGGTC CCGACAGTCT AGCTAGAGGA 
CTTGCCTTAG ACTCAACAAT GCCAGAATCG TTAGAATGTA GCATCTCCAG AGATGATGGA 
AGAGATGGGG CAGACAGCAG AGCCATAAAA AGATCCTTCT CCTCCAGCAA GCCACACCTA 
AAGCCTGCTC TAGGCATGAA TGGAGCCAAA GCTCGGAGTC AGAGCTTCAG CATCCACTTG 
GGAGAGAAGC CTTCCACACC CCCAACAGAA GGGCCAGGCA AGATTCGAAC TCAGATTATC 
ACCAACACGG CTGAAAGAGG CAACTCCCTC ACCAGACAGA ATATCTCAAT GGAAGGGTCC 
CCAAGCAAGA CCCCCTCTGT CTCAACATCT GATAATCTGC CCAATGCTGG AAGGCCCCTG 
GGAAACCCCT TTTCCAGGCA GAGTTCTCTC TGTAGCACCG GAAGTTCTAG CAGCCAGCAC 
GGAAGCCCAA GCAAGTTACC CTTGAGGATG CTCACAAGGC CGGAAGGACT CCTCACTCCT 
GGAGTGGAAG ACCAACGGCT CTCTACTCAG GAAGAGTGTC CTGAAAGAGG TGTACATGAG 
CAATCTAGCA GTGACCACCA TAGGCATCCA TCTCCTCTTT CAGACTGCCC TCAGTTCCTG 
CACGTTCCAG GGAGGATGCA GCGCCCAAGC AATTTGGAAA CAAGTGGGAT ATCTAAGCTA 
GAAACTTCTG GAAGGCATCC AGATGCTTCT TCAACTGGGA CTAGTGTCGT GAGCCCAGAA 
GCCCCCCTCT CACCCTCCAT CGAAGAAAAG GTCATGTTGT GCATTCAGGA GAATGTGGAA 
AAGGGCCAAG TGCAGACAAA GTCTGCCTCT GTGGAAGTGA AGCCCAGGGC AGGGCCTTCG 
TTCACCAGCT GGTTTGGTTT TCGAAAGAGC AGGCTTCCAG CTCTCAGTAG CAGGAGAATG 
GAAGCTTCCA AGAGTAAAGT GGAAAAGAAA GACACAAAAC TGAAATCTGA GAGGAAAAAA 
GAAAAGAAGA AAGCTGAAGT ACAATCAAAG ATAGAAAACG AACTGAGTAG GGGGACCAAG 
AAGGCAGATG GTCAGAGTCC AGACGATGGT CTGCAAAGCT CAGAGAGTCT GAAAGTATCC 
CAAGACATTT ATAACCAAAT GAAGTTGGAG CCAAGGAATA GACCCAGCCC TGCTGTGTGC 
CCAACAAAAG ATGCCTTCAT GACAGAACTT CTAAACAGAG TTGATAAGAG AGCCTCGGTA 
CAGAGAGAGA GTGGATCAAA GGGTGTTTCC TGCAGGAGCG TGTTAGAGGG CACCTCCCAG 
GGCTCCTGTT TCGCCAGCGG CTCTGTGAGC ACTCAAGGAA GTCAAAAGAA AAACATCAAA 
ACCAAAGTCG ATATGGAAAA ACCAAGAGAA TCCTTGGGGG CCGAGGTCAA TGAAGATGTA 
CAAGAAGATG AAGAAGATAG AGTTGCTGAC ACTACTCTCC AGAGCCACAT GATAGAATCC 
AACTGCCAGA TGAGAACACT GGACAGCGGG ATTGGAACCT TTCCCCTCCC AGACTCAGGA 
AACCGCTCAG TGGGACGATA CATATGCCAG CAGGACTCTC CAGAGGACAC TGATGCCCTC 
CTGCCTCTCC AGCCAGCCGC TTCCATGGCA TCCTCTGCCA GAGCCCAAAC CCTTGACCGC 
GAAGTGCCTT CCTCCACAGA CAGCCAGAGC TCCCCGGAGT CCATGATCAT CCATTCCATA 
TCTGACCCCA GCATGATGGC CAGAGAGATG AGAGCCTCAC AGAGTCACCT TCCCAAGCCT 
ACCTCCTCAG GAAAACCTAG TTTTGAAAAG CAAAATGGAG CAGAGTCAAA GGCTCACACT 
GGCCCATCAT TCGAATATGC TGAGGATGCA GTGGCAAGTG AGCCCCTTTC AGGATGGAGG 
GGTGAAGATG CTACTACTGA GACCCAGAAC AGGTGCCCTG AGGAAGCAGA AATCCTTCAC 
AGACTTGAAA GTGACTCGAG GACAGTTAAT CGTCACCGTG CTCTAGTGAG GCAGGAACCG 
AAACAAGTTG AAGAAACAAA AGAAGATCCT GAGAATAGAT TATCTGAAAT TTCCCTGGAG 
TCATTCAATA AATTTAGCAG CAATACTGTG ATTTTATTAG GAAAAGAGAA GTCTCCAAGT 
GAGGTTGAAG GACAAAAGGA AGAGAAAGGC AAAAAGACAG AAGTGTCTTC GAGTAGCTCC 
GAACGGCCTG GGGTGGACCG CGTGGAGTCT CTGAGTGACT CTCTGTATGA CAGTTTCTCC 
TCTTGTGCAA GTCAAGCATC CAACGAGGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529333.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529396.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X11, mRNA.

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CloneID	OMu28060
Clone ID Related Accession (Same CDS sequence)	XM_006529333.3 , XM_006529333.4
Accession Version	XM_006529333.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529333.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG CAATCATCGC GGATCTGGGA TAAAAGACTA 
AGTATTGATT CTTCGCTCCC AAGTGGCTTC GCCAGCCCTG CAGATGAACT ACCTCCGACC 
CGTATCAAAG AAAGCCACAT TCTAGAGGGA CTGAGGAAGC TACAGAAGCG GAAAGTGTTC 
CTTGAATCAC CATCATTGGT GATAAGCAAA TGGGGTTATA AAGATTGCAT GAACTCGAAT 
GAAGGGATCT ATTCGCCAGG AATTAAAACC AGCAGCCTCA AGGAGTGTCC GCCCTGCCAA 
CCGTCTGGCA TGGGGATCCC CTGCCAGAAT CTCCATCAGA CATTCATCTA TGATGCAAAT 
TCTCATGAGG ATGCACATGA AGAACTCCCA TCCTCAGCCT TGCCACTAAC ATCTCCGAGC 
CAGGGCTACA GAATACAGGG CAGTAAATTG ACACACAGTG TTTCAGACAG TCTGTTTAAC 
TGGGAGCCAC ATAGGAAGTA CTTTGCAGAA GGTACAGCCT CAGTATATAC CAGAGAAATG 
CCTGAAAGGC TAGCTCGTTG TGCTAGCCAC TGCCCCTTGG AGAGGAAGCT ATGTCCCAGT 
ATACACCTGC CCTGGGTGCA AAGAGACAGG GACCCACACA GCCAAGTTCA TGGCCATGTG 
CCTCTGAACC TGCAGCTCTC AGATACCGAT GACAATGAGA CCCTAAACGA GCTACACATA 
GAGAGCAGTG ATGAGAGAAG CCCTTCAGAC CTGTCCTTGA CTGGGGACAC CGACAAGTCC 
ATGGAGAACC TAGAGGTGGG ATTCAGAAAG TCTGAAGGTG GCTCTCGGGA CATGGGAGAA 
GAAGCATGTC CCACCCACTT AGAGACTAGC CCGAAGGCAT TCAGTTTCAT CAAGCAACAA 
AGGGTTGTCA AGAGGACCTC TTCGGAAGAA TGTGTCACGG TCATATTTGA TGCAGAAAAT 
GGAGAGCCAG TGGAATTCAG CTCTCACCAG GCTGGAGTCG TCACTGTCAC CAGGGATGAA 
ATTTCCATCA GCTCAGCCCT TGCTGGGCCC TCAGAGGAAC TCACTGAACT TTCACCTCAG 
GAAATTACTC ACTTCCAGGC AGGAGCTACT GCAAGAGACT GTCCTTTTCT GAAGAGACCT 
GAAGAGGAGA CTGCGAAAAA CATTCCAAAA GATGAAAATC ATATTCTCAC AGCTCCGAAT 
GACTTCTTCA GCTCCCAGAC TATAATAACT CAGAATTCCC CAAGACCAAA GCTCGCCAAA 
CCCACACAGG GGGTAGCCTG GCAGAGTAAT TCTAGACCTG TTGGAAACAT GGGTGTCTAC 
ACAAAGCAAG GTCTGACAAA AATCCCTTCC AGAGGCAAGT CTTCACCTCA GAAATCCAAA 
CTTGTGGAGT CTATGCCCAC CATGCTGGTC CCCTCCAGTG CCCTAGTGAC TCCTGAAAAG 
ACACCAGCAT CCCCTATGGG GAAACTCTCA CAATGTAGAA AGGCAGAGAG CCCTGGTTCT 
CTCTGTGATG TACAGCCAGA ATCACACATT CCAAAACACC CTGCCCAGGT TCCTCATATC 
CCCAAAATTT CCAATAAGAA GAACTGGGTT CAGTGCCCCA GGAGTCAGAC TTCAGGGATC 
CAGTCACGGC ATCTCATGGG GAGAAGTGAC AGTAGTGAAA TGAGGTCCAA TGGCTGTGTC 
TCTGCGCCAC AGACAAGAAT GAGGTCTCCC TCCCCTCCAA CCCCTCCAGG GAGATCAGTG 
TCACTCCTGG TTAGACCCAG TTATGATTAC CCACCACTAC CATCACCTGC CAAACCTGAA 
ACTCAGGGCC CTGGAGACGC TGTAAGTCCA GGGTTGAAAT CCCCGCTGTT GAAAGGATCC 
TCTGCTCCTC TTGTCTCTTC CAGTCCAGTG ATGTCAGAAA TACAGAAGAA ACCATCTGTG 
GCCTCCAGAA AGTCTGCCTT CCCTCCAGCT CTTCCCTCAA CCCAAGCGGT AGTGCACACC 
AGATACCCTG AACACATAGC CTCCAGTCCA TTTGCTGTGA CGACTTCAGG GCCCCCAAAA 
GTCTCTCCAA AAAGAGGCAT CCCTAAACCT CCCCCTCACC ATGCACTAGG AACTGCCCAC 
ATGGACACAG ACTTACAGAC ACCCAAGACT TGTCCTTCAA GCTGTGAACT GCTAGAGGTG 
ACATCTTGTA AAAGCCTGTC TCCAGGGAGA AAAAGACAGT TGAGTGACAG CACTGTGATG 
CCCCACAGGC CTTCCTTCTC AGGAGTGAAT GAGTCCTTGA CACCTCAGGT TAACAGTCCA 
TCCTCCTCTT CCTCATCCTG TAGAAACCAA GGGTCCTCAC AAGGTGGCCA GAACTCTCCC 
GAGAAAGGAC CTAAAACTCG CCTCCCAGTA GGACTCAAAG TTCTCATGAA GTCTCCTCAG 
TTGCTCAAGA AAACTTCCTT GGTACCAGGG AAACAAGAAA AGGATAGTTT AAATGAAGCA 
TCTCGAAGCT GTGTAGCAGC AAACACGTCT AATCCTGAGG ACTCCAAAGA CCCTGCAAGT 
CTGGAGACCT CAAGAGATGA GAGAACCACA GCCTTACTAG GTGTGAGAGG TCCCGACAGT 
CTAGCTAGAG GACTTGCCTT AGACTCAACA ATGCCAGAAT CGTTAGAATG TAGCATCTCC 
AGAGATGATG GAAGAGATGG GGCAGACAGC AGAGCCATAA AAAGATCCTT CTCCTCCAGC 
AAGCCACACC TAAAGCCTGC TCTAGGCATG AATGGAGCCA AAGCTCGGAG TCAGAGCTTC 
AGCATCCACT TGGGAGAGAA GCCTTCCACA CCCCCAACAG AAGGGCCAGG CAAGATTCGA 
ACTCAGATTA TCACCAACAC GGCTGAAAGA GGCAACTCCC TCACCAGACA GAATATCTCA 
ATGGAAGGGT CCCCAAGCAA GACCCCCTCT GTCTCAACAT CTGATAATCT GCCCAATGCT 
GGAAGGCCCC TGGGAAACCC CTTTTCCAGG CAGAGTTCTC TCTGTAGCAC CGGAAGTTCT 
AGCAGCCAGC ACGGAAGCCC AAGCAAGTTA CCCTTGAGGA TGCTCACAAG GCCGGAAGGA 
CTCCTCACTC CTGGAGTGGA AGACCAACGG CTCTCTACTC AGGAAGAGTG TCCTGAAAGA 
GGTGTACATG AGCAATCTAG CAGTGACCAC CATAGGCATC CATCTCCTCT TTCAGACTGC 
CCTCAGTTCC TGCACGTTCC AGGGAGGATG CAGCGCCCAA GCAATTTGGA AACAAGTGGG 
ATATCTAAGC TAGAAACTTC TGGAAGGCAT CCAGATGCTT CTTCAACTGG GACTAGTGTC 
GTGAGCCCAG AAGCCCCCCT CTCACCCTCC ATCGAAGAAA AGGTCATGTT GTGCATTCAG 
GAGAATGTGG AAAAGGGCCA AGTGCAGACA AAGTCTGCCT CTGTGGAAGT GAAGCCCAGG 
GCAGGGCCTT CGTTCACCAG CTGGTTTGGT TTTCGAAAGA GCAGGCTTCC AGCTCTCAGT 
AGCAGGAGAA TGGAAGCTTC CAAGAGTAAA GTGGAAAAGA AAGACACAAA ACTGAAATCT 
GAGAGGAAAA AAGAAAAGAA GAAAGCTGAA GTACAATCAA AGATAGAAAA CGAACTGAGT 
AGGGGGACCA AGAAGGCAGA TGGTCAGAGT CCAGACGATG GTCTGCAAAG CTCAGAGAGT 
CTGAAAGTAT CCCAAGACAT TTATAACCAA ATGAAGTTGG AGCCAAGGAA TAGACCCAGC 
CCTGCTGTGT GCCCAACAAA AGATGCCTTC ATGACAGAAC TTCTAAACAG AGTTGATAAG 
AGAGCCTCGG TACAGAGAGA GAGTGGATCA AAGGGTGTTT CCTGCAGGAG CGTGTTAGAG 
GGCACCTCCC AGGGCTCCTG TTTCGCCAGC GGCTCTGTGA GCACTCAAGG AAGTCAAAAG 
AAAAACATCA AAACCAAAGT CGATATGGAA AAACCAAGAG AATCCTTGGG GGCCGAGGTC 
AATGAAGATG TACAAGAAGA TGAAGAAGAT AGAGTTGCTG ACACTACTCT CCAGAGCCAC 
ATGATAGAAT CCAACTGCCA GATGAGAACA CTGGACAGCG GGATTGGAAC CTTTCCCCTC 
CCAGACTCAG GAAACCGCTC AGTGGGACGA TACATATGCC AGCAGGACTC TCCAGAGGAC 
ACTGATGCCC TCCTGCCTCT CCAGCCAGCC GCTTCCATGG CATCCTCTGC CAGAGCCCAA 
ACCCTTGACC GCGAAGTGCC TTCCTCCACA GACAGCCAGA GCTCCCCGGA GTCCATGATC 
ATCCATTCCA TATCTGACCC CAGCATGATG GCCAGAGAGA TGAGAGCCTC ACAGAGTCAC 
CTTCCCAAGC CTACCTCCTC AGGAAAACCT AGTTTTGAAA AGCAAAATGG AGCAGAGTCA 
AAGGCTCACA CTGGCCCATC ATTCGAATAT GCTGAGGATG CAGTGGCAAG TGAGCCCCTT 
TCAGGATGGA GGGGTGAAGA TGCTACTACT GAGACCCAGG AACCGAAACA AGTTGAAGAA 
ACAAAAGAAG ATCCTGAGAA TAGATTATCT GAAATTTCCC TGGAGTCATT CAATAAATTT 
AGCAGCAATA CTGTGATTTT ATTAGGAAAA GAGAAGTCTC CAAGTGAGGT TGAAGGACAA 
AAGGAAGAGA AAGGCAAAAA GACAGAAGTG TCTTCGAGTA GCTCCGAACG GCCTGGGGTG 
GACCGCGTGG AGTCTCTGAG TGACTCTCTG TATGACAGTT TCTCCTCTTG TGCAAGTCAA 
GCATCCAACG AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529396.1
CDS	847..6384
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_006529333.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529333.3

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG CAATCATCGC GGATCTGGGA TAAAAGACTA 
AGTATTGATT CTTCGCTCCC AAGTGGCTTC GCCAGCCCTG CAGATGAACT ACCTCCGACC 
CGTATCAAAG AAAGCCACAT TCTAGAGGGA CTGAGGAAGC TACAGAAGCG GAAAGTGTTC 
CTTGAATCAC CATCATTGGT GATAAGCAAA TGGGGTTATA AAGATTGCAT GAACTCGAAT 
GAAGGGATCT ATTCGCCAGG AATTAAAACC AGCAGCCTCA AGGAGTGTCC GCCCTGCCAA 
CCGTCTGGCA TGGGGATCCC CTGCCAGAAT CTCCATCAGA CATTCATCTA TGATGCAAAT 
TCTCATGAGG ATGCACATGA AGAACTCCCA TCCTCAGCCT TGCCACTAAC ATCTCCGAGC 
CAGGGCTACA GAATACAGGG CAGTAAATTG ACACACAGTG TTTCAGACAG TCTGTTTAAC 
TGGGAGCCAC ATAGGAAGTA CTTTGCAGAA GGTACAGCCT CAGTATATAC CAGAGAAATG 
CCTGAAAGGC TAGCTCGTTG TGCTAGCCAC TGCCCCTTGG AGAGGAAGCT ATGTCCCAGT 
ATACACCTGC CCTGGGTGCA AAGAGACAGG GACCCACACA GCCAAGTTCA TGGCCATGTG 
CCTCTGAACC TGCAGCTCTC AGATACCGAT GACAATGAGA CCCTAAACGA GCTACACATA 
GAGAGCAGTG ATGAGAGAAG CCCTTCAGAC CTGTCCTTGA CTGGGGACAC CGACAAGTCC 
ATGGAGAACC TAGAGGTGGG ATTCAGAAAG TCTGAAGGTG GCTCTCGGGA CATGGGAGAA 
GAAGCATGTC CCACCCACTT AGAGACTAGC CCGAAGGCAT TCAGTTTCAT CAAGCAACAA 
AGGGTTGTCA AGAGGACCTC TTCGGAAGAA TGTGTCACGG TCATATTTGA TGCAGAAAAT 
GGAGAGCCAG TGGAATTCAG CTCTCACCAG GCTGGAGTCG TCACTGTCAC CAGGGATGAA 
ATTTCCATCA GCTCAGCCCT TGCTGGGCCC TCAGAGGAAC TCACTGAACT TTCACCTCAG 
GAAATTACTC ACTTCCAGGC AGGAGCTACT GCAAGAGACT GTCCTTTTCT GAAGAGACCT 
GAAGAGGAGA CTGCGAAAAA CATTCCAAAA GATGAAAATC ATATTCTCAC AGCTCCGAAT 
GACTTCTTCA GCTCCCAGAC TATAATAACT CAGAATTCCC CAAGACCAAA GCTCGCCAAA 
CCCACACAGG GGGTAGCCTG GCAGAGTAAT TCTAGACCTG TTGGAAACAT GGGTGTCTAC 
ACAAAGCAAG GTCTGACAAA AATCCCTTCC AGAGGCAAGT CTTCACCTCA GAAATCCAAA 
CTTGTGGAGT CTATGCCCAC CATGCTGGTC CCCTCCAGTG CCCTAGTGAC TCCTGAAAAG 
ACACCAGCAT CCCCTATGGG GAAACTCTCA CAATGTAGAA AGGCAGAGAG CCCTGGTTCT 
CTCTGTGATG TACAGCCAGA ATCACACATT CCAAAACACC CTGCCCAGGT TCCTCATATC 
CCCAAAATTT CCAATAAGAA GAACTGGGTT CAGTGCCCCA GGAGTCAGAC TTCAGGGATC 
CAGTCACGGC ATCTCATGGG GAGAAGTGAC AGTAGTGAAA TGAGGTCCAA TGGCTGTGTC 
TCTGCGCCAC AGACAAGAAT GAGGTCTCCC TCCCCTCCAA CCCCTCCAGG GAGATCAGTG 
TCACTCCTGG TTAGACCCAG TTATGATTAC CCACCACTAC CATCACCTGC CAAACCTGAA 
ACTCAGGGCC CTGGAGACGC TGTAAGTCCA GGGTTGAAAT CCCCGCTGTT GAAAGGATCC 
TCTGCTCCTC TTGTCTCTTC CAGTCCAGTG ATGTCAGAAA TACAGAAGAA ACCATCTGTG 
GCCTCCAGAA AGTCTGCCTT CCCTCCAGCT CTTCCCTCAA CCCAAGCGGT AGTGCACACC 
AGATACCCTG AACACATAGC CTCCAGTCCA TTTGCTGTGA CGACTTCAGG GCCCCCAAAA 
GTCTCTCCAA AAAGAGGCAT CCCTAAACCT CCCCCTCACC ATGCACTAGG AACTGCCCAC 
ATGGACACAG ACTTACAGAC ACCCAAGACT TGTCCTTCAA GCTGTGAACT GCTAGAGGTG 
ACATCTTGTA AAAGCCTGTC TCCAGGGAGA AAAAGACAGT TGAGTGACAG CACTGTGATG 
CCCCACAGGC CTTCCTTCTC AGGAGTGAAT GAGTCCTTGA CACCTCAGGT TAACAGTCCA 
TCCTCCTCTT CCTCATCCTG TAGAAACCAA GGGTCCTCAC AAGGTGGCCA GAACTCTCCC 
GAGAAAGGAC CTAAAACTCG CCTCCCAGTA GGACTCAAAG TTCTCATGAA GTCTCCTCAG 
TTGCTCAAGA AAACTTCCTT GGTACCAGGG AAACAAGAAA AGGATAGTTT AAATGAAGCA 
TCTCGAAGCT GTGTAGCAGC AAACACGTCT AATCCTGAGG ACTCCAAAGA CCCTGCAAGT 
CTGGAGACCT CAAGAGATGA GAGAACCACA GCCTTACTAG GTGTGAGAGG TCCCGACAGT 
CTAGCTAGAG GACTTGCCTT AGACTCAACA ATGCCAGAAT CGTTAGAATG TAGCATCTCC 
AGAGATGATG GAAGAGATGG GGCAGACAGC AGAGCCATAA AAAGATCCTT CTCCTCCAGC 
AAGCCACACC TAAAGCCTGC TCTAGGCATG AATGGAGCCA AAGCTCGGAG TCAGAGCTTC 
AGCATCCACT TGGGAGAGAA GCCTTCCACA CCCCCAACAG AAGGGCCAGG CAAGATTCGA 
ACTCAGATTA TCACCAACAC GGCTGAAAGA GGCAACTCCC TCACCAGACA GAATATCTCA 
ATGGAAGGGT CCCCAAGCAA GACCCCCTCT GTCTCAACAT CTGATAATCT GCCCAATGCT 
GGAAGGCCCC TGGGAAACCC CTTTTCCAGG CAGAGTTCTC TCTGTAGCAC CGGAAGTTCT 
AGCAGCCAGC ACGGAAGCCC AAGCAAGTTA CCCTTGAGGA TGCTCACAAG GCCGGAAGGA 
CTCCTCACTC CTGGAGTGGA AGACCAACGG CTCTCTACTC AGGAAGAGTG TCCTGAAAGA 
GGTGTACATG AGCAATCTAG CAGTGACCAC CATAGGCATC CATCTCCTCT TTCAGACTGC 
CCTCAGTTCC TGCACGTTCC AGGGAGGATG CAGCGCCCAA GCAATTTGGA AACAAGTGGG 
ATATCTAAGC TAGAAACTTC TGGAAGGCAT CCAGATGCTT CTTCAACTGG GACTAGTGTC 
GTGAGCCCAG AAGCCCCCCT CTCACCCTCC ATCGAAGAAA AGGTCATGTT GTGCATTCAG 
GAGAATGTGG AAAAGGGCCA AGTGCAGACA AAGTCTGCCT CTGTGGAAGT GAAGCCCAGG 
GCAGGGCCTT CGTTCACCAG CTGGTTTGGT TTTCGAAAGA GCAGGCTTCC AGCTCTCAGT 
AGCAGGAGAA TGGAAGCTTC CAAGAGTAAA GTGGAAAAGA AAGACACAAA ACTGAAATCT 
GAGAGGAAAA AAGAAAAGAA GAAAGCTGAA GTACAATCAA AGATAGAAAA CGAACTGAGT 
AGGGGGACCA AGAAGGCAGA TGGTCAGAGT CCAGACGATG GTCTGCAAAG CTCAGAGAGT 
CTGAAAGTAT CCCAAGACAT TTATAACCAA ATGAAGTTGG AGCCAAGGAA TAGACCCAGC 
CCTGCTGTGT GCCCAACAAA AGATGCCTTC ATGACAGAAC TTCTAAACAG AGTTGATAAG 
AGAGCCTCGG TACAGAGAGA GAGTGGATCA AAGGGTGTTT CCTGCAGGAG CGTGTTAGAG 
GGCACCTCCC AGGGCTCCTG TTTCGCCAGC GGCTCTGTGA GCACTCAAGG AAGTCAAAAG 
AAAAACATCA AAACCAAAGT CGATATGGAA AAACCAAGAG AATCCTTGGG GGCCGAGGTC 
AATGAAGATG TACAAGAAGA TGAAGAAGAT AGAGTTGCTG ACACTACTCT CCAGAGCCAC 
ATGATAGAAT CCAACTGCCA GATGAGAACA CTGGACAGCG GGATTGGAAC CTTTCCCCTC 
CCAGACTCAG GAAACCGCTC AGTGGGACGA TACATATGCC AGCAGGACTC TCCAGAGGAC 
ACTGATGCCC TCCTGCCTCT CCAGCCAGCC GCTTCCATGG CATCCTCTGC CAGAGCCCAA 
ACCCTTGACC GCGAAGTGCC TTCCTCCACA GACAGCCAGA GCTCCCCGGA GTCCATGATC 
ATCCATTCCA TATCTGACCC CAGCATGATG GCCAGAGAGA TGAGAGCCTC ACAGAGTCAC 
CTTCCCAAGC CTACCTCCTC AGGAAAACCT AGTTTTGAAA AGCAAAATGG AGCAGAGTCA 
AAGGCTCACA CTGGCCCATC ATTCGAATAT GCTGAGGATG CAGTGGCAAG TGAGCCCCTT 
TCAGGATGGA GGGGTGAAGA TGCTACTACT GAGACCCAGG AACCGAAACA AGTTGAAGAA 
ACAAAAGAAG ATCCTGAGAA TAGATTATCT GAAATTTCCC TGGAGTCATT CAATAAATTT 
AGCAGCAATA CTGTGATTTT ATTAGGAAAA GAGAAGTCTC CAAGTGAGGT TGAAGGACAA 
AAGGAAGAGA AAGGCAAAAA GACAGAAGTG TCTTCGAGTA GCTCCGAACG GCCTGGGGTG 
GACCGCGTGG AGTCTCTGAG TGACTCTCTG TATGACAGTT TCTCCTCTTG TGCAAGTCAA 
GCATCCAACG AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529334.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529397.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X12, mRNA.

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CloneID	OMu28061
Clone ID Related Accession (Same CDS sequence)	XM_006529334.3 , XM_006529335.4 , XM_006529335.3 , XM_006529334.4
Accession Version	XM_006529334.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529334.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGTAACCATG 
GGACATGTCA TCTTACAGGC GGTGATAACA CCTCACCCTG AGTCCCCTGT GGAAAGAAAG 
AGCCATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529397.1
CDS	846..6377
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQVTMGHVILQAVITPHPESPVERKSHD

Target ORF information:

RefSeq Version	XM_006529334.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529334.3

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGTAACCATG 
GGACATGTCA TCTTACAGGC GGTGATAACA CCTCACCCTG AGTCCCCTGT GGAAAGAAAG 
AGCCATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529335.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529398.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X13, mRNA.

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CloneID	OMu28061
Clone ID Related Accession (Same CDS sequence)	XM_006529334.3 , XM_006529335.4 , XM_006529335.3 , XM_006529334.4
Accession Version	XM_006529335.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529335.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGTAACCATG 
GGACATGTCA TCTTACAGGC GGTGATAACA CCTCACCCTG AGTCCCCTGT GGAAAGAAAG 
AGCCATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529398.1
CDS	846..6377
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQVTMGHVILQAVITPHPESPVERKSHD

Target ORF information:

RefSeq Version	XM_006529335.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529335.3

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGTAACCATG 
GGACATGTCA TCTTACAGGC GGTGATAACA CCTCACCCTG AGTCCCCTGT GGAAAGAAAG 
AGCCATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_006529336.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529399.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X14, mRNA.

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CloneID	OMu28062
Clone ID Related Accession (Same CDS sequence)	XM_006529336.3 , XM_006529336.4
Accession Version	XM_006529336.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529336.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGCGGTGATA 
ACACCTCACC CTGAGTCCCC TGTGGAAAGA AAGAGCCATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529399.1
CDS	845..6349
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQAVITPHPESPVERKSHD

Target ORF information:

RefSeq Version	XM_006529336.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529336.3

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GGCGGTGATA 
ACACCTCACC CTGAGTCCCC TGTGGAAAGA AAGAGCCATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_011247958.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246260.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X4, mRNA.

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CloneID	OMu72729
Clone ID Related Accession (Same CDS sequence)	XM_011247958.2 , XM_011247958.3
Accession Version	XM_011247958.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTA ACAAATGCAT CGAGCACCTG CTGGCACAAC TCAGGGAGCA ACACAGAAGT 
CTCTGGCGGG AGAAGCTGGC TGTGGCCAGG CTGCAGAGAG AAGTTGCCCA AAGGACAAGC 
CAAGGGGCCA TGCACGAGAA GCTGATACAT GAGTTGGAAG AGGAACGGCA CTTGCGGCTA 
CAGAGTGAGG AGCGGCTGCG GGAGGTGACC CTGGAGTCAG AGCGCAACAG GATCCAGATG 
CTCGGCCTGC AGCAGCAGTT TTCCAGAATG GAAGAAACTG TCCGAAGTCT GTTGCAGAGC 
CAAGGATCTC CAGAGCAGAA AAAGGAAGAA CCTGCCAAGA TCACAGCCTA CCAGGAAGGA 
CTACCTGATG AAGAGAGAAA AGAGCAAGCA GCACTGGGTG ATCTCCATGT GGTGGCAGCT 
GATGAGGACT CAAGAAGCGA GCACAGTAGT GTGGAGGAGG GAAAGGAAGA CAGCAGACTG 
CTCCTGAAGA GACTGAAGGC CCTGGAGGCG GAGAATTCAG CACTGGCACT GGAAAACGAG 
AATCAGAGAG AACAGTATGA GCGATGTCTT GATGAGGTAG CCAACCAAGT TGTTCGAGCT 
CTACTCACTC AAAAGGATCT GAGGGAGGAG TGTGTAAAGT TGAAAACAAG AGTGTTTGAC 
TTGGAGCAAC AGAACCGAGC ACTCAGCCTC CTGTTCCAAC AGCGAGTCAG ACCAGCTTCT 
GATGTGCTTC TCCAGGAACA CCTCCAGAAT GCAAAATCAG GAACCCCTGC CTTGAAACAC 
TCTGGCTCAG GGGTTGTCAT CCCTGATCAC CTCTGTCCTC GCAACAGCTG CAGCAGTGGC 
AGTGAGCTAT CGCTGTCAAG CACCTGCAGC GAGTACTCCA GTGGCTCCTC CTACACGTGG 
CAAGACAGGA AGCTCATTGG GAAAAGGCAA TCATCGCGGA TCTGGGATAA AAGACTAAGT 
ATTGATTCTT CGCTCCCAAG TGGCTTCGCC AGCCCTGCAG ATGAACTACC TCCGACCCGT 
ATCAAAGAAA GCCACATTCT AGAGGGACTG AGGAAGCTAC AGAAGCGGAA AGTGTTCCTT 
GAATCACCAT CATTGGTGAT AAGCAAATGG GGTTATAAAG ATTGCATGAA CTCGAATGAA 
GGGATCTATT CGCCAGGAAT TAAAACCAGC AGCCTCAAGG AGTGTCCGCC CTGCCAACCG 
TCTGGCATGG GGATCCCCTG CCAGAATCTC CATCAGACAT TCATCTATGA TGCAAATTCT 
CATGAGGATG CACATGAAGA ACTCCCATCC TCAGCCTTGC CACTAACATC TCCGAGCCAG 
GGCTACAGAA TACAGGGCAG TAAATTGACA CACAGTGTTT CAGACAGTCT GTTTAACTGG 
GAGCCACATA GGAAGTACTT TGCAGAAGGT ACAGCCTCAG TATATACCAG AGAAATGCCT 
GAAAGGCTAG CTCGTTGTGC TAGCCACTGC CCCTTGGAGA GGAAGCTATG TCCCAGTATA 
CACCTGCCCT GGGTGCAAAG AGACAGGGAC CCACACAGCC AAGTTCATGG CCATGTGCCT 
CTGAACCTGC AGCTCTCAGA TACCGATGAC AATGAGACCC TAAACGAGCT ACACATAGAG 
AGCAGTGATG AGAGAAGCCC TTCAGACCTG TCCTTGACTG GGGACACCGA CAAGTCCATG 
GAGAACCTAG AGGTGGGATT CAGAAAGTCT GAAGGTGGCT CTCGGGACAT GGGAGAAGAA 
GCATGTCCCA CCCACTTAGA GACTAGCCCG AAGGCATTCA GTTTCATCAA GCAACAAAGG 
GTTGTCAAGA GGACCTCTTC GGAAGAATGT GTCACGGTCA TATTTGATGC AGAAAATGGA 
GAGCCAGTGG AATTCAGCTC TCACCAGGCT GGAGTCGTCA CTGTCACCAG GGATGAAATT 
TCCATCAGCT CAGCCCTTGC TGGGCCCTCA GAGGAACTCA CTGAACTTTC ACCTCAGGAA 
ATTACTCACT TCCAGGCAGG AGCTACTGCA AGAGACTGTC CTTTTCTGAA GAGACCTGAA 
GAGGAGACTG CGAAAAACAT TCCAAAAGAT GAAAATCATA TTCTCACAGC TCCGAATGAC 
TTCTTCAGCT CCCAGACTAT AATAACTCAG AATTCCCCAA GACCAAAGCT CGCCAAACCC 
ACACAGGGGG TAGCCTGGCA GAGTAATTCT AGACCTGTTG GAAACATGGG TGTCTACACA 
AAGCAAGGTC TGACAAAAAT CCCTTCCAGA GGCAAGTCTT CACCTCAGAA ATCCAAACTT 
GTGGAGTCTA TGCCCACCAT GCTGGTCCCC TCCAGTGCCC TAGTGACTCC TGAAAAGACA 
CCAGCATCCC CTATGGGGAA ACTCTCACAA TGTAGAAAGG CAGAGAGCCC TGGTTCTCTC 
TGTGATGTAC AGCCAGAATC ACACATTCCA AAACACCCTG CCCAGGTTCC TCATATCCCC 
AAAATTTCCA ATAAGAAGAA CTGGGTTCAG TGCCCCAGGA GTCAGACTTC AGGGATCCAG 
TCACGGCATC TCATGGGGAG AAGTGACAGT AGTGAAATGA GGTCCAATGG CTGTGTCTCT 
GCGCCACAGA CAAGAATGAG GTCTCCCTCC CCTCCAACCC CTCCAGGGAG ATCAGTGTCA 
CTCCTGGTTA GACCCAGTTA TGATTACCCA CCACTACCAT CACCTGCCAA ACCTGAAACT 
CAGGGCCCTG GAGACGCTGT AAGTCCAGGG TTGAAATCCC CGCTGTTGAA AGGATCCTCT 
GCTCCTCTTG TCTCTTCCAG TCCAGTGATG TCAGAAATAC AGAAGAAACC ATCTGTGGCC 
TCCAGAAAGT CTGCCTTCCC TCCAGCTCTT CCCTCAACCC AAGCGGTAGT GCACACCAGA 
TACCCTGAAC ACATAGCCTC CAGTCCATTT GCTGTGACGA CTTCAGGGCC CCCAAAAGTC 
TCTCCAAAAA GAGGCATCCC TAAACCTCCC CCTCACCATG CACTAGGAAC TGCCCACATG 
GACACAGACT TACAGACACC CAAGACTTGT CCTTCAAGCT GTGAACTGCT AGAGGTGACA 
TCTTGTAAAA GCCTGTCTCC AGGGAGAAAA AGACAGTTGA GTGACAGCAC TGTGATGCCC 
CACAGGCCTT CCTTCTCAGG AGTGAATGAG TCCTTGACAC CTCAGGTTAA CAGTCCATCC 
TCCTCTTCCT CATCCTGTAG AAACCAAGGG TCCTCACAAG GTGGCCAGAA CTCTCCCGAG 
AAAGGACCTA AAACTCGCCT CCCAGTAGGA CTCAAAGTTC TCATGAAGTC TCCTCAGTTG 
CTCAAGAAAA CTTCCTTGGT ACCAGGGAAA CAAGAAAAGG ATAGTTTAAA TGAAGCATCT 
CGAAGCTGTG TAGCAGCAAA CACGTCTAAT CCTGAGGACT CCAAAGACCC TGCAAGTCTG 
GAGACCTCAA GAGATGAGAG AACCACAGCC TTACTAGGTG TGAGAGGTCC CGACAGTCTA 
GCTAGAGGAC TTGCCTTAGA CTCAACAATG CCAGAATCGT TAGAATGTAG CATCTCCAGA 
GATGATGGAA GAGATGGGGC AGACAGCAGA GCCATAAAAA GATCCTTCTC CTCCAGCAAG 
CCACACCTAA AGCCTGCTCT AGGCATGAAT GGAGCCAAAG CTCGGAGTCA GAGCTTCAGC 
ATCCACTTGG GAGAGAAGCC TTCCACACCC CCAACAGAAG GGCCAGGCAA GATTCGAACT 
CAGATTATCA CCAACACGGC TGAAAGAGGC AACTCCCTCA CCAGACAGAA TATCTCAATG 
GAAGGGTCCC CAAGCAAGAC CCCCTCTGTC TCAACATCTG ATAATCTGCC CAATGCTGGA 
AGGCCCCTGG GAAACCCCTT TTCCAGGCAG AGTTCTCTCT GTAGCACCGG AAGTTCTAGC 
AGCCAGCACG GAAGCCCAAG CAAGTTACCC TTGAGGATGC TCACAAGGCC GGAAGGACTC 
CTCACTCCTG GAGTGGAAGA CCAACGGCTC TCTACTCAGG AAGAGTGTCC TGAAAGAGGT 
GTACATGAGC AATCTAGCAG TGACCACCAT AGGCATCCAT CTCCTCTTTC AGACTGCCCT 
CAGTTCCTGC ACGTTCCAGG GAGGATGCAG CGCCCAAGCA ATTTGGAAAC AAGTGGGATA 
TCTAAGCTAG AAACTTCTGG AAGGCATCCA GATGCTTCTT CAACTGGGAC TAGTGTCGTG 
AGCCCAGAAG CCCCCCTCTC ACCCTCCATC GAAGAAAAGG TCATGTTGTG CATTCAGGAG 
AATGTGGAAA AGGGCCAAGT GCAGACAAAG TCTGCCTCTG TGGAAGTGAA GCCCAGGGCA 
GGGCCTTCGT TCACCAGCTG GTTTGGTTTT CGAAAGAGCA GGCTTCCAGC TCTCAGTAGC 
AGGAGAATGG AAGCTTCCAA GAGTAAAGTG GAAAAGAAAG ACACAAAACT GAAATCTGAG 
AGGAAAAAAG AAAAGAAGAA AGCTGAAGTA CAATCAAAGA TAGAAAACGA ACTGAGTAGG 
GGGACCAAGA AGGCAGATGG TCAGAGTCCA GACGATGGTC TGCAAAGCTC AGAGAGTCTG 
AAAGTATCCC AAGACATTTA TAACCAAATG AAGTTGGAGC CAAGGAATAG ACCCAGCCCT 
GCTGTGTGCC CAACAAAAGA TGCCTTCATG ACAGAACTTC TAAACAGAGT TGATAAGAGA 
GCCTCGGTAC AGAGAGAGAG TGGATCAAAG GGTGTTTCCT GCAGGAGCGT GTTAGAGGGC 
ACCTCCCAGG GCTCCTGTTT CGCCAGCGGC TCTGTGAGCA CTCAAGGAAG TCAAAAGAAA 
AACATCAAAA CCAAAGTCGA TATGGAAAAA CCAAGAGAAT CCTTGGGGGC CGAGGTCAAT 
GAAGATGTAC AAGAAGATGA AGAAGATAGA GTTGCTGACA CTACTCTCCA GAGCCACATG 
ATAGAATCCA ACTGCCAGAT GAGAACACTG GACAGCGGGA TTGGAACCTT TCCCCTCCCA 
GACTCAGGAA ACCGCTCAGT GGGACGATAC ATATGCCAGC AGGACTCTCC AGAGGACACT 
GATGCCCTCC TGCCTCTCCA GCCAGCCGCT TCCATGGCAT CCTCTGCCAG AGCCCAAACC 
CTTGACCGCG AAGTGCCTTC CTCCACAGAC AGCCAGAGCT CCCCGGAGTC CATGATCATC 
CATTCCATAT CTGACCCCAG CATGATGGCC AGAGAGATGA GAGCCTCACA GAGTCACCTT 
CCCAAGCCTA CCTCCTCAGG AAAACCTAGT TTTGAAAAGC AAAATGGAGC AGAGTCAAAG 
GCTCACACTG GCCCATCATT CGAATATGCT GAGGATGCAG TGGCAAGTGA GCCCCTTTCA 
GGATGGAGGG GTGAAGATGC TACTACTGAG ACCCAGAACA GGTGCCCTGA GGAAGCAGAA 
ATCCTTCACA GACTTGAAAG TGACTCGAGG ACAGTTAATC GTCACCGTGC TCTAGTGAGG 
CAGGAACCGA AACAAGTTGA AGAAACAAAA GAAGATCCTG AGAATAGATT ATCTGAAATT 
TCCCTGGAGT CATTCAATAA ATTTAGCAGC AATACTGTGA TTTTATTAGG AAAAGAGAAG 
TCTCCAAGTG AGGTTGAAGG ACAAAAGGAA GAGAAAGGCA AAAAGACAGA AGTGTCTTCG 
AGTAGCTCCG AACGGCCTGG GGTGGACCGC GTGGAGTCTC TGAGTGACTC TCTGTATGAC 
AGTTTCTCCT CTTGTGCAAG TCAAGCATCC AACGAGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246260.1
CDS	3277..9018
Translation	MDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_011247958.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247958.2

ORF Insert Sequence:

ATGGACTCTA ACAAATGCAT CGAGCACCTG CTGGCACAAC TCAGGGAGCA ACACAGAAGT 
CTCTGGCGGG AGAAGCTGGC TGTGGCCAGG CTGCAGAGAG AAGTTGCCCA AAGGACAAGC 
CAAGGGGCCA TGCACGAGAA GCTGATACAT GAGTTGGAAG AGGAACGGCA CTTGCGGCTA 
CAGAGTGAGG AGCGGCTGCG GGAGGTGACC CTGGAGTCAG AGCGCAACAG GATCCAGATG 
CTCGGCCTGC AGCAGCAGTT TTCCAGAATG GAAGAAACTG TCCGAAGTCT GTTGCAGAGC 
CAAGGATCTC CAGAGCAGAA AAAGGAAGAA CCTGCCAAGA TCACAGCCTA CCAGGAAGGA 
CTACCTGATG AAGAGAGAAA AGAGCAAGCA GCACTGGGTG ATCTCCATGT GGTGGCAGCT 
GATGAGGACT CAAGAAGCGA GCACAGTAGT GTGGAGGAGG GAAAGGAAGA CAGCAGACTG 
CTCCTGAAGA GACTGAAGGC CCTGGAGGCG GAGAATTCAG CACTGGCACT GGAAAACGAG 
AATCAGAGAG AACAGTATGA GCGATGTCTT GATGAGGTAG CCAACCAAGT TGTTCGAGCT 
CTACTCACTC AAAAGGATCT GAGGGAGGAG TGTGTAAAGT TGAAAACAAG AGTGTTTGAC 
TTGGAGCAAC AGAACCGAGC ACTCAGCCTC CTGTTCCAAC AGCGAGTCAG ACCAGCTTCT 
GATGTGCTTC TCCAGGAACA CCTCCAGAAT GCAAAATCAG GAACCCCTGC CTTGAAACAC 
TCTGGCTCAG GGGTTGTCAT CCCTGATCAC CTCTGTCCTC GCAACAGCTG CAGCAGTGGC 
AGTGAGCTAT CGCTGTCAAG CACCTGCAGC GAGTACTCCA GTGGCTCCTC CTACACGTGG 
CAAGACAGGA AGCTCATTGG GAAAAGGCAA TCATCGCGGA TCTGGGATAA AAGACTAAGT 
ATTGATTCTT CGCTCCCAAG TGGCTTCGCC AGCCCTGCAG ATGAACTACC TCCGACCCGT 
ATCAAAGAAA GCCACATTCT AGAGGGACTG AGGAAGCTAC AGAAGCGGAA AGTGTTCCTT 
GAATCACCAT CATTGGTGAT AAGCAAATGG GGTTATAAAG ATTGCATGAA CTCGAATGAA 
GGGATCTATT CGCCAGGAAT TAAAACCAGC AGCCTCAAGG AGTGTCCGCC CTGCCAACCG 
TCTGGCATGG GGATCCCCTG CCAGAATCTC CATCAGACAT TCATCTATGA TGCAAATTCT 
CATGAGGATG CACATGAAGA ACTCCCATCC TCAGCCTTGC CACTAACATC TCCGAGCCAG 
GGCTACAGAA TACAGGGCAG TAAATTGACA CACAGTGTTT CAGACAGTCT GTTTAACTGG 
GAGCCACATA GGAAGTACTT TGCAGAAGGT ACAGCCTCAG TATATACCAG AGAAATGCCT 
GAAAGGCTAG CTCGTTGTGC TAGCCACTGC CCCTTGGAGA GGAAGCTATG TCCCAGTATA 
CACCTGCCCT GGGTGCAAAG AGACAGGGAC CCACACAGCC AAGTTCATGG CCATGTGCCT 
CTGAACCTGC AGCTCTCAGA TACCGATGAC AATGAGACCC TAAACGAGCT ACACATAGAG 
AGCAGTGATG AGAGAAGCCC TTCAGACCTG TCCTTGACTG GGGACACCGA CAAGTCCATG 
GAGAACCTAG AGGTGGGATT CAGAAAGTCT GAAGGTGGCT CTCGGGACAT GGGAGAAGAA 
GCATGTCCCA CCCACTTAGA GACTAGCCCG AAGGCATTCA GTTTCATCAA GCAACAAAGG 
GTTGTCAAGA GGACCTCTTC GGAAGAATGT GTCACGGTCA TATTTGATGC AGAAAATGGA 
GAGCCAGTGG AATTCAGCTC TCACCAGGCT GGAGTCGTCA CTGTCACCAG GGATGAAATT 
TCCATCAGCT CAGCCCTTGC TGGGCCCTCA GAGGAACTCA CTGAACTTTC ACCTCAGGAA 
ATTACTCACT TCCAGGCAGG AGCTACTGCA AGAGACTGTC CTTTTCTGAA GAGACCTGAA 
GAGGAGACTG CGAAAAACAT TCCAAAAGAT GAAAATCATA TTCTCACAGC TCCGAATGAC 
TTCTTCAGCT CCCAGACTAT AATAACTCAG AATTCCCCAA GACCAAAGCT CGCCAAACCC 
ACACAGGGGG TAGCCTGGCA GAGTAATTCT AGACCTGTTG GAAACATGGG TGTCTACACA 
AAGCAAGGTC TGACAAAAAT CCCTTCCAGA GGCAAGTCTT CACCTCAGAA ATCCAAACTT 
GTGGAGTCTA TGCCCACCAT GCTGGTCCCC TCCAGTGCCC TAGTGACTCC TGAAAAGACA 
CCAGCATCCC CTATGGGGAA ACTCTCACAA TGTAGAAAGG CAGAGAGCCC TGGTTCTCTC 
TGTGATGTAC AGCCAGAATC ACACATTCCA AAACACCCTG CCCAGGTTCC TCATATCCCC 
AAAATTTCCA ATAAGAAGAA CTGGGTTCAG TGCCCCAGGA GTCAGACTTC AGGGATCCAG 
TCACGGCATC TCATGGGGAG AAGTGACAGT AGTGAAATGA GGTCCAATGG CTGTGTCTCT 
GCGCCACAGA CAAGAATGAG GTCTCCCTCC CCTCCAACCC CTCCAGGGAG ATCAGTGTCA 
CTCCTGGTTA GACCCAGTTA TGATTACCCA CCACTACCAT CACCTGCCAA ACCTGAAACT 
CAGGGCCCTG GAGACGCTGT AAGTCCAGGG TTGAAATCCC CGCTGTTGAA AGGATCCTCT 
GCTCCTCTTG TCTCTTCCAG TCCAGTGATG TCAGAAATAC AGAAGAAACC ATCTGTGGCC 
TCCAGAAAGT CTGCCTTCCC TCCAGCTCTT CCCTCAACCC AAGCGGTAGT GCACACCAGA 
TACCCTGAAC ACATAGCCTC CAGTCCATTT GCTGTGACGA CTTCAGGGCC CCCAAAAGTC 
TCTCCAAAAA GAGGCATCCC TAAACCTCCC CCTCACCATG CACTAGGAAC TGCCCACATG 
GACACAGACT TACAGACACC CAAGACTTGT CCTTCAAGCT GTGAACTGCT AGAGGTGACA 
TCTTGTAAAA GCCTGTCTCC AGGGAGAAAA AGACAGTTGA GTGACAGCAC TGTGATGCCC 
CACAGGCCTT CCTTCTCAGG AGTGAATGAG TCCTTGACAC CTCAGGTTAA CAGTCCATCC 
TCCTCTTCCT CATCCTGTAG AAACCAAGGG TCCTCACAAG GTGGCCAGAA CTCTCCCGAG 
AAAGGACCTA AAACTCGCCT CCCAGTAGGA CTCAAAGTTC TCATGAAGTC TCCTCAGTTG 
CTCAAGAAAA CTTCCTTGGT ACCAGGGAAA CAAGAAAAGG ATAGTTTAAA TGAAGCATCT 
CGAAGCTGTG TAGCAGCAAA CACGTCTAAT CCTGAGGACT CCAAAGACCC TGCAAGTCTG 
GAGACCTCAA GAGATGAGAG AACCACAGCC TTACTAGGTG TGAGAGGTCC CGACAGTCTA 
GCTAGAGGAC TTGCCTTAGA CTCAACAATG CCAGAATCGT TAGAATGTAG CATCTCCAGA 
GATGATGGAA GAGATGGGGC AGACAGCAGA GCCATAAAAA GATCCTTCTC CTCCAGCAAG 
CCACACCTAA AGCCTGCTCT AGGCATGAAT GGAGCCAAAG CTCGGAGTCA GAGCTTCAGC 
ATCCACTTGG GAGAGAAGCC TTCCACACCC CCAACAGAAG GGCCAGGCAA GATTCGAACT 
CAGATTATCA CCAACACGGC TGAAAGAGGC AACTCCCTCA CCAGACAGAA TATCTCAATG 
GAAGGGTCCC CAAGCAAGAC CCCCTCTGTC TCAACATCTG ATAATCTGCC CAATGCTGGA 
AGGCCCCTGG GAAACCCCTT TTCCAGGCAG AGTTCTCTCT GTAGCACCGG AAGTTCTAGC 
AGCCAGCACG GAAGCCCAAG CAAGTTACCC TTGAGGATGC TCACAAGGCC GGAAGGACTC 
CTCACTCCTG GAGTGGAAGA CCAACGGCTC TCTACTCAGG AAGAGTGTCC TGAAAGAGGT 
GTACATGAGC AATCTAGCAG TGACCACCAT AGGCATCCAT CTCCTCTTTC AGACTGCCCT 
CAGTTCCTGC ACGTTCCAGG GAGGATGCAG CGCCCAAGCA ATTTGGAAAC AAGTGGGATA 
TCTAAGCTAG AAACTTCTGG AAGGCATCCA GATGCTTCTT CAACTGGGAC TAGTGTCGTG 
AGCCCAGAAG CCCCCCTCTC ACCCTCCATC GAAGAAAAGG TCATGTTGTG CATTCAGGAG 
AATGTGGAAA AGGGCCAAGT GCAGACAAAG TCTGCCTCTG TGGAAGTGAA GCCCAGGGCA 
GGGCCTTCGT TCACCAGCTG GTTTGGTTTT CGAAAGAGCA GGCTTCCAGC TCTCAGTAGC 
AGGAGAATGG AAGCTTCCAA GAGTAAAGTG GAAAAGAAAG ACACAAAACT GAAATCTGAG 
AGGAAAAAAG AAAAGAAGAA AGCTGAAGTA CAATCAAAGA TAGAAAACGA ACTGAGTAGG 
GGGACCAAGA AGGCAGATGG TCAGAGTCCA GACGATGGTC TGCAAAGCTC AGAGAGTCTG 
AAAGTATCCC AAGACATTTA TAACCAAATG AAGTTGGAGC CAAGGAATAG ACCCAGCCCT 
GCTGTGTGCC CAACAAAAGA TGCCTTCATG ACAGAACTTC TAAACAGAGT TGATAAGAGA 
GCCTCGGTAC AGAGAGAGAG TGGATCAAAG GGTGTTTCCT GCAGGAGCGT GTTAGAGGGC 
ACCTCCCAGG GCTCCTGTTT CGCCAGCGGC TCTGTGAGCA CTCAAGGAAG TCAAAAGAAA 
AACATCAAAA CCAAAGTCGA TATGGAAAAA CCAAGAGAAT CCTTGGGGGC CGAGGTCAAT 
GAAGATGTAC AAGAAGATGA AGAAGATAGA GTTGCTGACA CTACTCTCCA GAGCCACATG 
ATAGAATCCA ACTGCCAGAT GAGAACACTG GACAGCGGGA TTGGAACCTT TCCCCTCCCA 
GACTCAGGAA ACCGCTCAGT GGGACGATAC ATATGCCAGC AGGACTCTCC AGAGGACACT 
GATGCCCTCC TGCCTCTCCA GCCAGCCGCT TCCATGGCAT CCTCTGCCAG AGCCCAAACC 
CTTGACCGCG AAGTGCCTTC CTCCACAGAC AGCCAGAGCT CCCCGGAGTC CATGATCATC 
CATTCCATAT CTGACCCCAG CATGATGGCC AGAGAGATGA GAGCCTCACA GAGTCACCTT 
CCCAAGCCTA CCTCCTCAGG AAAACCTAGT TTTGAAAAGC AAAATGGAGC AGAGTCAAAG 
GCTCACACTG GCCCATCATT CGAATATGCT GAGGATGCAG TGGCAAGTGA GCCCCTTTCA 
GGATGGAGGG GTGAAGATGC TACTACTGAG ACCCAGAACA GGTGCCCTGA GGAAGCAGAA 
ATCCTTCACA GACTTGAAAG TGACTCGAGG ACAGTTAATC GTCACCGTGC TCTAGTGAGG 
CAGGAACCGA AACAAGTTGA AGAAACAAAA GAAGATCCTG AGAATAGATT ATCTGAAATT 
TCCCTGGAGT CATTCAATAA ATTTAGCAGC AATACTGTGA TTTTATTAGG AAAAGAGAAG 
TCTCCAAGTG AGGTTGAAGG ACAAAAGGAA GAGAAAGGCA AAAAGACAGA AGTGTCTTCG 
AGTAGCTCCG AACGGCCTGG GGTGGACCGC GTGGAGTCTC TGAGTGACTC TCTGTATGAC 
AGTTTCTCCT CTTGTGCAAG TCAAGCATCC AACGAGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_011247959.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246261.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X6, mRNA.

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CloneID	OMu72730
Clone ID Related Accession (Same CDS sequence)	XM_011247959.2
Accession Version	XM_011247959.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247959.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCCC GCAGGCCAGC TTGCAGAGTT GGCTTTGCTG GCTGGGAGAA GCTGGCTGTG 
GCCAGGCTGC AGAGAGAAGT TGCCCAAAGG ACAAGCCAAG GGGCCATGCA CGAGAAGCTG 
ATACATGAGT TGGAAGAGGA ACGGCACTTG CGGCTACAGA GTGAGGAGCG GCTGCGGGAG 
GTGACCCTGG AGTCAGAGCG CAACAGGATC CAGATGCTCG GCCTGCAGCA GCAGTTTTCC 
AGAATGGAAG AAACTGTCCG AAGTCTGTTG CAGAGCCAAG GATCTCCAGA GCAGAAAAAG 
GAAGAACCTG CCAAGATCAC AGCCTACCAG GAAGGACTAC CTGATGAAGA GAGAAAAGAG 
CAAGCAGCAC TGGGTGATCT CCATGTGGTG GCAGCTGATG AGGACTCAAG AAGCGAGCAC 
AGTAGTGTGG AGGAGGGAAA GGAAGACAGC AGACTGCTCC TGAAGAGACT GAAGGCCCTG 
GAGGCGGAGA ATTCAGCACT GGCACTGGAA AACGAGAATC AGAGAGAACA GTATGAGCGA 
TGTCTTGATG AGGTAGCCAA CCAAGTTGTT CGAGCTCTAC TCACTCAAAA GGATCTGAGG 
GAGGAGTGTG TAAAGTTGAA AACAAGAGTG TTTGACTTGG AGCAACAGAA CCGAGCACTC 
AGCCTCCTGT TCCAACAGCG AGTCAGACCA GCTTCTGATG TGCTTCTCCA GGAACACCTC 
CAGAATGCAA AATCAGGAAC CCCTGCCTTG AAACACTCTG GCTCAGGGGT TGTCATCCCT 
GATCACCTCT GTCCTCGCAA CAGCTGCAGC AGTGGCAGTG AGCTATCGCT GTCAAGCACC 
TGCAGCGAGT ACTCCAGTGG CTCCTCCTAC ACGTGGCAAG ACAGGAAGCT CATTGGGAAA 
AGGCAATCAT CGCGGATCTG GGATAAAAGA CTAAGTATTG ATTCTTCGCT CCCAAGTGGC 
TTCGCCAGCC CTGCAGATGA ACTACCTCCG ACCCGTATCA AAGAAAGCCA CATTCTAGAG 
GGACTGAGGA AGCTACAGAA GCGGAAAGTG TTCCTTGAAT CACCATCATT GGTGATAAGC 
AAATGGGGTT ATAAAGATTG CATGAACTCG AATGAAGGGA TCTATTCGCC AGGAATTAAA 
ACCAGCAGCC TCAAGGAGTG TCCGCCCTGC CAACCGTCTG GCATGGGGAT CCCCTGCCAG 
AATCTCCATC AGACATTCAT CTATGATGCA AATTCTCATG AGGATGCACA TGAAGAACTC 
CCATCCTCAG CCTTGCCACT AACATCTCCG AGCCAGGGCT ACAGAATACA GGGCAGTAAA 
TTGACACACA GTGTTTCAGA CAGTCTGTTT AACTGGGAGC CACATAGGAA GTACTTTGCA 
GAAGGTACAG CCTCAGTATA TACCAGAGAA ATGCCTGAAA GGCTAGCTCG TTGTGCTAGC 
CACTGCCCCT TGGAGAGGAA GCTATGTCCC AGTATACACC TGCCCTGGGT GCAAAGAGAC 
AGGGACCCAC ACAGCCAAGT TCATGGCCAT GTGCCTCTGA ACCTGCAGCT CTCAGATACC 
GATGACAATG AGACCCTAAA CGAGCTACAC ATAGAGAGCA GTGATGAGAG AAGCCCTTCA 
GACCTGTCCT TGACTGGGGA CACCGACAAG TCCATGGAGA ACCTAGAGGT GGGATTCAGA 
AAGTCTGAAG GTGGCTCTCG GGACATGGGA GAAGAAGCAT GTCCCACCCA CTTAGAGACT 
AGCCCGAAGG CATTCAGTTT CATCAAGCAA CAAAGGGTTG TCAAGAGGAC CTCTTCGGAA 
GAATGTGTCA CGGTCATATT TGATGCAGAA AATGGAGAGC CAGTGGAATT CAGCTCTCAC 
CAGGCTGGAG TCGTCACTGT CACCAGGGAT GAAATTTCCA TCAGCTCAGC CCTTGCTGGG 
CCCTCAGAGG AACTCACTGA ACTTTCACCT CAGGAAATTA CTCACTTCCA GGCAGGAGCT 
ACTGCAAGAG ACTGTCCTTT TCTGAAGAGA CCTGAAGAGG AGACTGCGAA AAACATTCCA 
AAAGATGAAA ATCATATTCT CACAGCTCCG AATGACTTCT TCAGCTCCCA GACTATAATA 
ACTCAGAATT CCCCAAGACC AAAGCTCGCC AAACCCACAC AGGGGGTAGC CTGGCAGAGT 
AATTCTAGAC CTGTTGGAAA CATGGGTGTC TACACAAAGC AAGGTCTGAC AAAAATCCCT 
TCCAGAGGCA AGTCTTCACC TCAGAAATCC AAACTTGTGG AGTCTATGCC CACCATGCTG 
GTCCCCTCCA GTGCCCTAGT GACTCCTGAA AAGACACCAG CATCCCCTAT GGGGAAACTC 
TCACAATGTA GAAAGGCAGA GAGCCCTGGT TCTCTCTGTG ATGTACAGCC AGAATCACAC 
ATTCCAAAAC ACCCTGCCCA GGTTCCTCAT ATCCCCAAAA TTTCCAATAA GAAGAACTGG 
GTTCAGTGCC CCAGGAGTCA GACTTCAGGG ATCCAGTCAC GGCATCTCAT GGGGAGAAGT 
GACAGTAGTG AAATGAGGTC CAATGGCTGT GTCTCTGCGC CACAGACAAG AATGAGGTCT 
CCCTCCCCTC CAACCCCTCC AGGGAGATCA GTGTCACTCC TGGTTAGACC CAGTTATGAT 
TACCCACCAC TACCATCACC TGCCAAACCT GAAACTCAGG GCCCTGGAGA CGCTGTAAGT 
CCAGGGTTGA AATCCCCGCT GTTGAAAGGA TCCTCTGCTC CTCTTGTCTC TTCCAGTCCA 
GTGATGTCAG AAATACAGAA GAAACCATCT GTGGCCTCCA GAAAGTCTGC CTTCCCTCCA 
GCTCTTCCCT CAACCCAAGC GGTAGTGCAC ACCAGATACC CTGAACACAT AGCCTCCAGT 
CCATTTGCTG TGACGACTTC AGGGCCCCCA AAAGTCTCTC CAAAAAGAGG CATCCCTAAA 
CCTCCCCCTC ACCATGCACT AGGAACTGCC CACATGGACA CAGACTTACA GACACCCAAG 
ACTTGTCCTT CAAGCTGTGA ACTGCTAGAG GTGACATCTT GTAAAAGCCT GTCTCCAGGG 
AGAAAAAGAC AGTTGAGTGA CAGCACTGTG ATGCCCCACA GGCCTTCCTT CTCAGGAGTG 
AATGAGTCCT TGACACCTCA GGTTAACAGT CCATCCTCCT CTTCCTCATC CTGTAGAAAC 
CAAGGGTCCT CACAAGGTGG CCAGAACTCT CCCGAGAAAG GACCTAAAAC TCGCCTCCCA 
GTAGGACTCA AAGTTCTCAT GAAGTCTCCT CAGTTGCTCA AGAAAACTTC CTTGGTACCA 
GGGAAACAAG AAAAGGATAG TTTAAATGAA GCATCTCGAA GCTGTGTAGC AGCAAACACG 
TCTAATCCTG AGGACTCCAA AGACCCTGCA AGTCTGGAGA CCTCAAGAGA TGAGAGAACC 
ACAGCCTTAC TAGGTGTGAG AGGTCCCGAC AGTCTAGCTA GAGGACTTGC CTTAGACTCA 
ACAATGCCAG AATCGTTAGA ATGTAGCATC TCCAGAGATG ATGGAAGAGA TGGGGCAGAC 
AGCAGAGCCA TAAAAAGATC CTTCTCCTCC AGCAAGCCAC ACCTAAAGCC TGCTCTAGGC 
ATGAATGGAG CCAAAGCTCG GAGTCAGAGC TTCAGCATCC ACTTGGGAGA GAAGCCTTCC 
ACACCCCCAA CAGAAGGGCC AGGCAAGATT CGAACTCAGA TTATCACCAA CACGGCTGAA 
AGAGGCAACT CCCTCACCAG ACAGAATATC TCAATGGAAG GGTCCCCAAG CAAGACCCCC 
TCTGTCTCAA CATCTGATAA TCTGCCCAAT GCTGGAAGGC CCCTGGGAAA CCCCTTTTCC 
AGGCAGAGTT CTCTCTGTAG CACCGGAAGT TCTAGCAGCC AGCACGGAAG CCCAAGCAAG 
TTACCCTTGA GGATGCTCAC AAGGCCGGAA GGACTCCTCA CTCCTGGAGT GGAAGACCAA 
CGGCTCTCTA CTCAGGAAGA GTGTCCTGAA AGAGGTGTAC ATGAGCAATC TAGCAGTGAC 
CACCATAGGC ATCCATCTCC TCTTTCAGAC TGCCCTCAGT TCCTGCACGT TCCAGGGAGG 
ATGCAGCGCC CAAGCAATTT GGAAACAAGT GGGATATCTA AGCTAGAAAC TTCTGGAAGG 
CATCCAGATG CTTCTTCAAC TGGGACTAGT GTCGTGAGCC CAGAAGCCCC CCTCTCACCC 
TCCATCGAAG AAAAGGTCAT GTTGTGCATT CAGGAGAATG TGGAAAAGGG CCAAGTGCAG 
ACAAAGTCTG CCTCTGTGGA AGTGAAGCCC AGGGCAGGGC CTTCGTTCAC CAGCTGGTTT 
GGTTTTCGAA AGAGCAGGCT TCCAGCTCTC AGTAGCAGGA GAATGGAAGC TTCCAAGAGT 
AAAGTGGAAA AGAAAGACAC AAAACTGAAA TCTGAGAGGA AAAAAGAAAA GAAGAAAGCT 
GAAGTACAAT CAAAGATAGA AAACGAACTG AGTAGGGGGA CCAAGAAGGC AGATGGTCAG 
AGTCCAGACG ATGGTCTGCA AAGCTCAGAG AGTCTGAAAG TATCCCAAGA CATTTATAAC 
CAAATGAAGT TGGAGCCAAG GAATAGACCC AGCCCTGCTG TGTGCCCAAC AAAAGATGCC 
TTCATGACAG AACTTCTAAA CAGAGTTGAT AAGAGAGCCT CGGTACAGAG AGAGAGTGGA 
TCAAAGGGTG TTTCCTGCAG GAGCGTGTTA GAGGGCACCT CCCAGGGCTC CTGTTTCGCC 
AGCGGCTCTG TGAGCACTCA AGGAAGTCAA AAGAAAAACA TCAAAACCAA AGTCGATATG 
GAAAAACCAA GAGAATCCTT GGGGGCCGAG GTCAATGAAG ATGTACAAGA AGATGAAGAA 
GATAGAGTTG CTGACACTAC TCTCCAGAGC CACATGATAG AATCCAACTG CCAGATGAGA 
ACACTGGACA GCGGGATTGG AACCTTTCCC CTCCCAGACT CAGGAAACCG CTCAGTGGGA 
CGATACATAT GCCAGCAGGA CTCTCCAGAG GACACTGATG CCCTCCTGCC TCTCCAGCCA 
GCCGCTTCCA TGGCATCCTC TGCCAGAGCC CAAACCCTTG ACCGCGAAGT GCCTTCCTCC 
ACAGACAGCC AGAGCTCCCC GGAGTCCATG ATCATCCATT CCATATCTGA CCCCAGCATG 
ATGGCCAGAG AGATGAGAGC CTCACAGAGT CACCTTCCCA AGCCTACCTC CTCAGGAAAA 
CCTAGTTTTG AAAAGCAAAA TGGAGCAGAG TCAAAGGCTC ACACTGGCCC ATCATTCGAA 
TATGCTGAGG ATGCAGTGGC AAGTGAGCCC CTTTCAGGAT GGAGGGGTGA AGATGCTACT 
ACTGAGACCC AGAACAGGTG CCCTGAGGAA GCAGAAATCC TTCACAGACT TGAAAGTGAC 
TCGAGGACAG TTAATCGTCA CCGTGCTCTA GTGAGGCAGG AACCGAAACA AGTTGAAGAA 
ACAAAAGAAG ATCCTGAGAA TAGATTATCT GAAATTTCCC TGGAGTCATT CAATAAATTT 
AGCAGCAATA CTGTGATTTT ATTAGGAAAA GAGAAGTCTC CAAGTGAGGT TGAAGGACAA 
AAGGAAGAGA AAGGCAAAAA GACAGAAGTG TCTTCGAGTA GCTCCGAACG GCCTGGGGTG 
GACCGCGTGG AGTCTCTGAG TGACTCTCTG TATGACAGTT TCTCCTCTTG TGCAAGTCAA 
GCATCCAACG AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246261.1
CDS	414..6131
Translation	MLSRRPACRVGFAGWEKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_011247959.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247959.2

ORF Insert Sequence:

ATGCTCTCCC GCAGGCCAGC TTGCAGAGTT GGCTTTGCTG GCTGGGAGAA GCTGGCTGTG 
GCCAGGCTGC AGAGAGAAGT TGCCCAAAGG ACAAGCCAAG GGGCCATGCA CGAGAAGCTG 
ATACATGAGT TGGAAGAGGA ACGGCACTTG CGGCTACAGA GTGAGGAGCG GCTGCGGGAG 
GTGACCCTGG AGTCAGAGCG CAACAGGATC CAGATGCTCG GCCTGCAGCA GCAGTTTTCC 
AGAATGGAAG AAACTGTCCG AAGTCTGTTG CAGAGCCAAG GATCTCCAGA GCAGAAAAAG 
GAAGAACCTG CCAAGATCAC AGCCTACCAG GAAGGACTAC CTGATGAAGA GAGAAAAGAG 
CAAGCAGCAC TGGGTGATCT CCATGTGGTG GCAGCTGATG AGGACTCAAG AAGCGAGCAC 
AGTAGTGTGG AGGAGGGAAA GGAAGACAGC AGACTGCTCC TGAAGAGACT GAAGGCCCTG 
GAGGCGGAGA ATTCAGCACT GGCACTGGAA AACGAGAATC AGAGAGAACA GTATGAGCGA 
TGTCTTGATG AGGTAGCCAA CCAAGTTGTT CGAGCTCTAC TCACTCAAAA GGATCTGAGG 
GAGGAGTGTG TAAAGTTGAA AACAAGAGTG TTTGACTTGG AGCAACAGAA CCGAGCACTC 
AGCCTCCTGT TCCAACAGCG AGTCAGACCA GCTTCTGATG TGCTTCTCCA GGAACACCTC 
CAGAATGCAA AATCAGGAAC CCCTGCCTTG AAACACTCTG GCTCAGGGGT TGTCATCCCT 
GATCACCTCT GTCCTCGCAA CAGCTGCAGC AGTGGCAGTG AGCTATCGCT GTCAAGCACC 
TGCAGCGAGT ACTCCAGTGG CTCCTCCTAC ACGTGGCAAG ACAGGAAGCT CATTGGGAAA 
AGGCAATCAT CGCGGATCTG GGATAAAAGA CTAAGTATTG ATTCTTCGCT CCCAAGTGGC 
TTCGCCAGCC CTGCAGATGA ACTACCTCCG ACCCGTATCA AAGAAAGCCA CATTCTAGAG 
GGACTGAGGA AGCTACAGAA GCGGAAAGTG TTCCTTGAAT CACCATCATT GGTGATAAGC 
AAATGGGGTT ATAAAGATTG CATGAACTCG AATGAAGGGA TCTATTCGCC AGGAATTAAA 
ACCAGCAGCC TCAAGGAGTG TCCGCCCTGC CAACCGTCTG GCATGGGGAT CCCCTGCCAG 
AATCTCCATC AGACATTCAT CTATGATGCA AATTCTCATG AGGATGCACA TGAAGAACTC 
CCATCCTCAG CCTTGCCACT AACATCTCCG AGCCAGGGCT ACAGAATACA GGGCAGTAAA 
TTGACACACA GTGTTTCAGA CAGTCTGTTT AACTGGGAGC CACATAGGAA GTACTTTGCA 
GAAGGTACAG CCTCAGTATA TACCAGAGAA ATGCCTGAAA GGCTAGCTCG TTGTGCTAGC 
CACTGCCCCT TGGAGAGGAA GCTATGTCCC AGTATACACC TGCCCTGGGT GCAAAGAGAC 
AGGGACCCAC ACAGCCAAGT TCATGGCCAT GTGCCTCTGA ACCTGCAGCT CTCAGATACC 
GATGACAATG AGACCCTAAA CGAGCTACAC ATAGAGAGCA GTGATGAGAG AAGCCCTTCA 
GACCTGTCCT TGACTGGGGA CACCGACAAG TCCATGGAGA ACCTAGAGGT GGGATTCAGA 
AAGTCTGAAG GTGGCTCTCG GGACATGGGA GAAGAAGCAT GTCCCACCCA CTTAGAGACT 
AGCCCGAAGG CATTCAGTTT CATCAAGCAA CAAAGGGTTG TCAAGAGGAC CTCTTCGGAA 
GAATGTGTCA CGGTCATATT TGATGCAGAA AATGGAGAGC CAGTGGAATT CAGCTCTCAC 
CAGGCTGGAG TCGTCACTGT CACCAGGGAT GAAATTTCCA TCAGCTCAGC CCTTGCTGGG 
CCCTCAGAGG AACTCACTGA ACTTTCACCT CAGGAAATTA CTCACTTCCA GGCAGGAGCT 
ACTGCAAGAG ACTGTCCTTT TCTGAAGAGA CCTGAAGAGG AGACTGCGAA AAACATTCCA 
AAAGATGAAA ATCATATTCT CACAGCTCCG AATGACTTCT TCAGCTCCCA GACTATAATA 
ACTCAGAATT CCCCAAGACC AAAGCTCGCC AAACCCACAC AGGGGGTAGC CTGGCAGAGT 
AATTCTAGAC CTGTTGGAAA CATGGGTGTC TACACAAAGC AAGGTCTGAC AAAAATCCCT 
TCCAGAGGCA AGTCTTCACC TCAGAAATCC AAACTTGTGG AGTCTATGCC CACCATGCTG 
GTCCCCTCCA GTGCCCTAGT GACTCCTGAA AAGACACCAG CATCCCCTAT GGGGAAACTC 
TCACAATGTA GAAAGGCAGA GAGCCCTGGT TCTCTCTGTG ATGTACAGCC AGAATCACAC 
ATTCCAAAAC ACCCTGCCCA GGTTCCTCAT ATCCCCAAAA TTTCCAATAA GAAGAACTGG 
GTTCAGTGCC CCAGGAGTCA GACTTCAGGG ATCCAGTCAC GGCATCTCAT GGGGAGAAGT 
GACAGTAGTG AAATGAGGTC CAATGGCTGT GTCTCTGCGC CACAGACAAG AATGAGGTCT 
CCCTCCCCTC CAACCCCTCC AGGGAGATCA GTGTCACTCC TGGTTAGACC CAGTTATGAT 
TACCCACCAC TACCATCACC TGCCAAACCT GAAACTCAGG GCCCTGGAGA CGCTGTAAGT 
CCAGGGTTGA AATCCCCGCT GTTGAAAGGA TCCTCTGCTC CTCTTGTCTC TTCCAGTCCA 
GTGATGTCAG AAATACAGAA GAAACCATCT GTGGCCTCCA GAAAGTCTGC CTTCCCTCCA 
GCTCTTCCCT CAACCCAAGC GGTAGTGCAC ACCAGATACC CTGAACACAT AGCCTCCAGT 
CCATTTGCTG TGACGACTTC AGGGCCCCCA AAAGTCTCTC CAAAAAGAGG CATCCCTAAA 
CCTCCCCCTC ACCATGCACT AGGAACTGCC CACATGGACA CAGACTTACA GACACCCAAG 
ACTTGTCCTT CAAGCTGTGA ACTGCTAGAG GTGACATCTT GTAAAAGCCT GTCTCCAGGG 
AGAAAAAGAC AGTTGAGTGA CAGCACTGTG ATGCCCCACA GGCCTTCCTT CTCAGGAGTG 
AATGAGTCCT TGACACCTCA GGTTAACAGT CCATCCTCCT CTTCCTCATC CTGTAGAAAC 
CAAGGGTCCT CACAAGGTGG CCAGAACTCT CCCGAGAAAG GACCTAAAAC TCGCCTCCCA 
GTAGGACTCA AAGTTCTCAT GAAGTCTCCT CAGTTGCTCA AGAAAACTTC CTTGGTACCA 
GGGAAACAAG AAAAGGATAG TTTAAATGAA GCATCTCGAA GCTGTGTAGC AGCAAACACG 
TCTAATCCTG AGGACTCCAA AGACCCTGCA AGTCTGGAGA CCTCAAGAGA TGAGAGAACC 
ACAGCCTTAC TAGGTGTGAG AGGTCCCGAC AGTCTAGCTA GAGGACTTGC CTTAGACTCA 
ACAATGCCAG AATCGTTAGA ATGTAGCATC TCCAGAGATG ATGGAAGAGA TGGGGCAGAC 
AGCAGAGCCA TAAAAAGATC CTTCTCCTCC AGCAAGCCAC ACCTAAAGCC TGCTCTAGGC 
ATGAATGGAG CCAAAGCTCG GAGTCAGAGC TTCAGCATCC ACTTGGGAGA GAAGCCTTCC 
ACACCCCCAA CAGAAGGGCC AGGCAAGATT CGAACTCAGA TTATCACCAA CACGGCTGAA 
AGAGGCAACT CCCTCACCAG ACAGAATATC TCAATGGAAG GGTCCCCAAG CAAGACCCCC 
TCTGTCTCAA CATCTGATAA TCTGCCCAAT GCTGGAAGGC CCCTGGGAAA CCCCTTTTCC 
AGGCAGAGTT CTCTCTGTAG CACCGGAAGT TCTAGCAGCC AGCACGGAAG CCCAAGCAAG 
TTACCCTTGA GGATGCTCAC AAGGCCGGAA GGACTCCTCA CTCCTGGAGT GGAAGACCAA 
CGGCTCTCTA CTCAGGAAGA GTGTCCTGAA AGAGGTGTAC ATGAGCAATC TAGCAGTGAC 
CACCATAGGC ATCCATCTCC TCTTTCAGAC TGCCCTCAGT TCCTGCACGT TCCAGGGAGG 
ATGCAGCGCC CAAGCAATTT GGAAACAAGT GGGATATCTA AGCTAGAAAC TTCTGGAAGG 
CATCCAGATG CTTCTTCAAC TGGGACTAGT GTCGTGAGCC CAGAAGCCCC CCTCTCACCC 
TCCATCGAAG AAAAGGTCAT GTTGTGCATT CAGGAGAATG TGGAAAAGGG CCAAGTGCAG 
ACAAAGTCTG CCTCTGTGGA AGTGAAGCCC AGGGCAGGGC CTTCGTTCAC CAGCTGGTTT 
GGTTTTCGAA AGAGCAGGCT TCCAGCTCTC AGTAGCAGGA GAATGGAAGC TTCCAAGAGT 
AAAGTGGAAA AGAAAGACAC AAAACTGAAA TCTGAGAGGA AAAAAGAAAA GAAGAAAGCT 
GAAGTACAAT CAAAGATAGA AAACGAACTG AGTAGGGGGA CCAAGAAGGC AGATGGTCAG 
AGTCCAGACG ATGGTCTGCA AAGCTCAGAG AGTCTGAAAG TATCCCAAGA CATTTATAAC 
CAAATGAAGT TGGAGCCAAG GAATAGACCC AGCCCTGCTG TGTGCCCAAC AAAAGATGCC 
TTCATGACAG AACTTCTAAA CAGAGTTGAT AAGAGAGCCT CGGTACAGAG AGAGAGTGGA 
TCAAAGGGTG TTTCCTGCAG GAGCGTGTTA GAGGGCACCT CCCAGGGCTC CTGTTTCGCC 
AGCGGCTCTG TGAGCACTCA AGGAAGTCAA AAGAAAAACA TCAAAACCAA AGTCGATATG 
GAAAAACCAA GAGAATCCTT GGGGGCCGAG GTCAATGAAG ATGTACAAGA AGATGAAGAA 
GATAGAGTTG CTGACACTAC TCTCCAGAGC CACATGATAG AATCCAACTG CCAGATGAGA 
ACACTGGACA GCGGGATTGG AACCTTTCCC CTCCCAGACT CAGGAAACCG CTCAGTGGGA 
CGATACATAT GCCAGCAGGA CTCTCCAGAG GACACTGATG CCCTCCTGCC TCTCCAGCCA 
GCCGCTTCCA TGGCATCCTC TGCCAGAGCC CAAACCCTTG ACCGCGAAGT GCCTTCCTCC 
ACAGACAGCC AGAGCTCCCC GGAGTCCATG ATCATCCATT CCATATCTGA CCCCAGCATG 
ATGGCCAGAG AGATGAGAGC CTCACAGAGT CACCTTCCCA AGCCTACCTC CTCAGGAAAA 
CCTAGTTTTG AAAAGCAAAA TGGAGCAGAG TCAAAGGCTC ACACTGGCCC ATCATTCGAA 
TATGCTGAGG ATGCAGTGGC AAGTGAGCCC CTTTCAGGAT GGAGGGGTGA AGATGCTACT 
ACTGAGACCC AGAACAGGTG CCCTGAGGAA GCAGAAATCC TTCACAGACT TGAAAGTGAC 
TCGAGGACAG TTAATCGTCA CCGTGCTCTA GTGAGGCAGG AACCGAAACA AGTTGAAGAA 
ACAAAAGAAG ATCCTGAGAA TAGATTATCT GAAATTTCCC TGGAGTCATT CAATAAATTT 
AGCAGCAATA CTGTGATTTT ATTAGGAAAA GAGAAGTCTC CAAGTGAGGT TGAAGGACAA 
AAGGAAGAGA AAGGCAAAAA GACAGAAGTG TCTTCGAGTA GCTCCGAACG GCCTGGGGTG 
GACCGCGTGG AGTCTCTGAG TGACTCTCTG TATGACAGTT TCTCCTCTTG TGCAAGTCAA 
GCATCCAACG AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_011247960.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246262.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X7, mRNA.

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CloneID	OMu72731
Clone ID Related Accession (Same CDS sequence)	XM_011247960.2 , XM_011247960.3
Accession Version	XM_011247960.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247960.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAT CAAGCCAACC CAGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GAACAGGTGC 
CCTGAGGAAG CAGAAATCCT TCACAGACTT GAAAGTGACT CGAGGACAGT TAATCGTCAC 
CGTGCTCTAG TGAGGCAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246262.1
CDS	1681..7377
Translation	MMTSSQPREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_011247960.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247960.2

ORF Insert Sequence:

ATGATGACAT CAAGCCAACC CAGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GAACAGGTGC 
CCTGAGGAAG CAGAAATCCT TCACAGACTT GAAAGTGACT CGAGGACAGT TAATCGTCAC 
CGTGCTCTAG TGAGGCAGGA ACCGAAACAA GTTGAAGAAA CAAAAGAAGA TCCTGAGAAT 
AGATTATCTG AAATTTCCCT GGAGTCATTC AATAAATTTA GCAGCAATAC TGTGATTTTA 
TTAGGAAAAG AGAAGTCTCC AAGTGAGGTT GAAGGACAAA AGGAAGAGAA AGGCAAAAAG 
ACAGAAGTGT CTTCGAGTAG CTCCGAACGG CCTGGGGTGG ACCGCGTGGA GTCTCTGAGT 
GACTCTCTGT ATGACAGTTT CTCCTCTTGT GCAAGTCAAG CATCCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_011247962.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246264.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X15, mRNA.

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CloneID	OMu72732
Clone ID Related Accession (Same CDS sequence)	XM_030252635.1 , XM_011247962.2
Accession Version	XM_011247962.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247962.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246264.1
CDS	845..6298
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQ

Target ORF information:

RefSeq Version	XM_011247962.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247962.2

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG GAACACCTCC AGAATGCAAA ATCAGGAACC 
CCTGCCTTGA AACACTCTGG CTCAGGGGTT GTCATCCCTG ATCACCTCTG TCCTCGCAAC 
AGCTGCAGCA GTGGCAGTGA GCTATCGCTG TCAAGCACCT GCAGCGAGTA CTCCAGTGGC 
TCCTCCTACA CGTGGCAAGA CAGGAAGCTC ATTGGGAAAA GGCAATCATC GCGGATCTGG 
GATAAAAGAC TAAGTATTGA TTCTTCGCTC CCAAGTGGCT TCGCCAGCCC TGCAGATGAA 
CTACCTCCGA CCCGTATCAA AGAAAGCCAC ATTCTAGAGG GACTGAGGAA GCTACAGAAG 
CGGAAAGTGT TCCTTGAATC ACCATCATTG GTGATAAGCA AATGGGGTTA TAAAGATTGC 
ATGAACTCGA ATGAAGGGAT CTATTCGCCA GGAATTAAAA CCAGCAGCCT CAAGGAGTGT 
CCGCCCTGCC AACCGTCTGG CATGGGGATC CCCTGCCAGA ATCTCCATCA GACATTCATC 
TATGATGCAA ATTCTCATGA GGATGCACAT GAAGAACTCC CATCCTCAGC CTTGCCACTA 
ACATCTCCGA GCCAGGGCTA CAGAATACAG GGCAGTAAAT TGACACACAG TGTTTCAGAC 
AGTCTGTTTA ACTGGGAGCC ACATAGGAAG TACTTTGCAG AAGGTACAGC CTCAGTATAT 
ACCAGAGAAA TGCCTGAAAG GCTAGCTCGT TGTGCTAGCC ACTGCCCCTT GGAGAGGAAG 
CTATGTCCCA GTATACACCT GCCCTGGGTG CAAAGAGACA GGGACCCACA CAGCCAAGTT 
CATGGCCATG TGCCTCTGAA CCTGCAGCTC TCAGATACCG ATGACAATGA GACCCTAAAC 
GAGCTACACA TAGAGAGCAG TGATGAGAGA AGCCCTTCAG ACCTGTCCTT GACTGGGGAC 
ACCGACAAGT CCATGGAGAA CCTAGAGGTG GGATTCAGAA AGTCTGAAGG TGGCTCTCGG 
GACATGGGAG AAGAAGCATG TCCCACCCAC TTAGAGACTA GCCCGAAGGC ATTCAGTTTC 
ATCAAGCAAC AAAGGGTTGT CAAGAGGACC TCTTCGGAAG AATGTGTCAC GGTCATATTT 
GATGCAGAAA ATGGAGAGCC AGTGGAATTC AGCTCTCACC AGGCTGGAGT CGTCACTGTC 
ACCAGGGATG AAATTTCCAT CAGCTCAGCC CTTGCTGGGC CCTCAGAGGA ACTCACTGAA 
CTTTCACCTC AGGAAATTAC TCACTTCCAG GCAGGAGCTA CTGCAAGAGA CTGTCCTTTT 
CTGAAGAGAC CTGAAGAGGA GACTGCGAAA AACATTCCAA AAGATGAAAA TCATATTCTC 
ACAGCTCCGA ATGACTTCTT CAGCTCCCAG ACTATAATAA CTCAGAATTC CCCAAGACCA 
AAGCTCGCCA AACCCACACA GGGGGTAGCC TGGCAGAGTA ATTCTAGACC TGTTGGAAAC 
ATGGGTGTCT ACACAAAGCA AGGTCTGACA AAAATCCCTT CCAGAGGCAA GTCTTCACCT 
CAGAAATCCA AACTTGTGGA GTCTATGCCC ACCATGCTGG TCCCCTCCAG TGCCCTAGTG 
ACTCCTGAAA AGACACCAGC ATCCCCTATG GGGAAACTCT CACAATGTAG AAAGGCAGAG 
AGCCCTGGTT CTCTCTGTGA TGTACAGCCA GAATCACACA TTCCAAAACA CCCTGCCCAG 
GTTCCTCATA TCCCCAAAAT TTCCAATAAG AAGAACTGGG TTCAGTGCCC CAGGAGTCAG 
ACTTCAGGGA TCCAGTCACG GCATCTCATG GGGAGAAGTG ACAGTAGTGA AATGAGGTCC 
AATGGCTGTG TCTCTGCGCC ACAGACAAGA ATGAGGTCTC CCTCCCCTCC AACCCCTCCA 
GGGAGATCAG TGTCACTCCT GGTTAGACCC AGTTATGATT ACCCACCACT ACCATCACCT 
GCCAAACCTG AAACTCAGGG CCCTGGAGAC GCTGTAAGTC CAGGGTTGAA ATCCCCGCTG 
TTGAAAGGAT CCTCTGCTCC TCTTGTCTCT TCCAGTCCAG TGATGTCAGA AATACAGAAG 
AAACCATCTG TGGCCTCCAG AAAGTCTGCC TTCCCTCCAG CTCTTCCCTC AACCCAAGCG 
GTAGTGCACA CCAGATACCC TGAACACATA GCCTCCAGTC CATTTGCTGT GACGACTTCA 
GGGCCCCCAA AAGTCTCTCC AAAAAGAGGC ATCCCTAAAC CTCCCCCTCA CCATGCACTA 
GGAACTGCCC ACATGGACAC AGACTTACAG ACACCCAAGA CTTGTCCTTC AAGCTGTGAA 
CTGCTAGAGG TGACATCTTG TAAAAGCCTG TCTCCAGGGA GAAAAAGACA GTTGAGTGAC 
AGCACTGTGA TGCCCCACAG GCCTTCCTTC TCAGGAGTGA ATGAGTCCTT GACACCTCAG 
GTTAACAGTC CATCCTCCTC TTCCTCATCC TGTAGAAACC AAGGGTCCTC ACAAGGTGGC 
CAGAACTCTC CCGAGAAAGG ACCTAAAACT CGCCTCCCAG TAGGACTCAA AGTTCTCATG 
AAGTCTCCTC AGTTGCTCAA GAAAACTTCC TTGGTACCAG GGAAACAAGA AAAGGATAGT 
TTAAATGAAG CATCTCGAAG CTGTGTAGCA GCAAACACGT CTAATCCTGA GGACTCCAAA 
GACCCTGCAA GTCTGGAGAC CTCAAGAGAT GAGAGAACCA CAGCCTTACT AGGTGTGAGA 
GGTCCCGACA GTCTAGCTAG AGGACTTGCC TTAGACTCAA CAATGCCAGA ATCGTTAGAA 
TGTAGCATCT CCAGAGATGA TGGAAGAGAT GGGGCAGACA GCAGAGCCAT AAAAAGATCC 
TTCTCCTCCA GCAAGCCACA CCTAAAGCCT GCTCTAGGCA TGAATGGAGC CAAAGCTCGG 
AGTCAGAGCT TCAGCATCCA CTTGGGAGAG AAGCCTTCCA CACCCCCAAC AGAAGGGCCA 
GGCAAGATTC GAACTCAGAT TATCACCAAC ACGGCTGAAA GAGGCAACTC CCTCACCAGA 
CAGAATATCT CAATGGAAGG GTCCCCAAGC AAGACCCCCT CTGTCTCAAC ATCTGATAAT 
CTGCCCAATG CTGGAAGGCC CCTGGGAAAC CCCTTTTCCA GGCAGAGTTC TCTCTGTAGC 
ACCGGAAGTT CTAGCAGCCA GCACGGAAGC CCAAGCAAGT TACCCTTGAG GATGCTCACA 
AGGCCGGAAG GACTCCTCAC TCCTGGAGTG GAAGACCAAC GGCTCTCTAC TCAGGAAGAG 
TGTCCTGAAA GAGGTGTACA TGAGCAATCT AGCAGTGACC ACCATAGGCA TCCATCTCCT 
CTTTCAGACT GCCCTCAGTT CCTGCACGTT CCAGGGAGGA TGCAGCGCCC AAGCAATTTG 
GAAACAAGTG GGATATCTAA GCTAGAAACT TCTGGAAGGC ATCCAGATGC TTCTTCAACT 
GGGACTAGTG TCGTGAGCCC AGAAGCCCCC CTCTCACCCT CCATCGAAGA AAAGGTCATG 
TTGTGCATTC AGGAGAATGT GGAAAAGGGC CAAGTGCAGA CAAAGTCTGC CTCTGTGGAA 
GTGAAGCCCA GGGCAGGGCC TTCGTTCACC AGCTGGTTTG GTTTTCGAAA GAGCAGGCTT 
CCAGCTCTCA GTAGCAGGAG AATGGAAGCT TCCAAGAGTA AAGTGGAAAA GAAAGACACA 
AAACTGAAAT CTGAGAGGAA AAAAGAAAAG AAGAAAGCTG AAGTACAATC AAAGATAGAA 
AACGAACTGA GTAGGGGGAC CAAGAAGGCA GATGGTCAGA GTCCAGACGA TGGTCTGCAA 
AGCTCAGAGA GTCTGAAAGT ATCCCAAGAC ATTTATAACC AAATGAAGTT GGAGCCAAGG 
AATAGACCCA GCCCTGCTGT GTGCCCAACA AAAGATGCCT TCATGACAGA ACTTCTAAAC 
AGAGTTGATA AGAGAGCCTC GGTACAGAGA GAGAGTGGAT CAAAGGGTGT TTCCTGCAGG 
AGCGTGTTAG AGGGCACCTC CCAGGGCTCC TGTTTCGCCA GCGGCTCTGT GAGCACTCAA 
GGAAGTCAAA AGAAAAACAT CAAAACCAAA GTCGATATGG AAAAACCAAG AGAATCCTTG 
GGGGCCGAGG TCAATGAAGA TGTACAAGAA GATGAAGAAG ATAGAGTTGC TGACACTACT 
CTCCAGAGCC ACATGATAGA ATCCAACTGC CAGATGAGAA CACTGGACAG CGGGATTGGA 
ACCTTTCCCC TCCCAGACTC AGGAAACCGC TCAGTGGGAC GATACATATG CCAGCAGGAC 
TCTCCAGAGG ACACTGATGC CCTCCTGCCT CTCCAGCCAG CCGCTTCCAT GGCATCCTCT 
GCCAGAGCCC AAACCCTTGA CCGCGAAGTG CCTTCCTCCA CAGACAGCCA GAGCTCCCCG 
GAGTCCATGA TCATCCATTC CATATCTGAC CCCAGCATGA TGGCCAGAGA GATGAGAGCC 
TCACAGAGTC ACCTTCCCAA GCCTACCTCC TCAGGAAAAC CTAGTTTTGA AAAGCAAAAT 
GGAGCAGAGT CAAAGGCTCA CACTGGCCCA TCATTCGAAT ATGCTGAGGA TGCAGTGGCA 
AGTGAGCCCC TTTCAGGATG GAGGGGTGAA GATGCTACTA CTGAGACCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap5 ( XM_011247963.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246265.1 Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X16, mRNA.

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CloneID	OMu72733
Clone ID Related Accession (Same CDS sequence)	XM_011247963.2 , XM_011247963.3
Accession Version	XM_011247963.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nck-associated protein 5 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247963.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG AAACGCCACT CACATGTCAT GGCTCTCTCA 
TCTGGTGATC TGCTCTCAGA GGCAGGAGGA AGCCAAAGTC TGGCACTGTC GTGGACAGAT 
ATTGAGCTGC ATGAACACCT CCAGAATGCA AAATCAGGAA CCCCTGCCTT GAAACACTCT 
GGCTCAGGGG TTGTCATCCC TGATCACCTC TGTCCTCGCA ACAGCTGCAG CAGTGGCAGT 
GAGCTATCGC TGTCAAGCAC CTGCAGCGAG TACTCCAGTG GCTCCTCCTA CACGTGGCAA 
GACAGGAAGC TCATTGGGAA AAGGCAATCA TCGCGGATCT GGGATAAAAG ACTAAGTATT 
GATTCTTCGC TCCCAAGTGG CTTCGCCAGC CCTGCAGATG AACTACCTCC GACCCGTATC 
AAAGAAAGCC ACATTCTAGA GGGACTGAGG AAGCTACAGA AGCGGAAAGT GTTCCTTGAA 
TCACCATCAT TGGTGATAAG CAAATGGGGT TATAAAGATT GCATGAACTC GAATGAAGGG 
ATCTATTCGC CAGGAATTAA AACCAGCAGC CTCAAGGAGT GTCCGCCCTG CCAACCGTCT 
GGCATGGGGA TCCCCTGCCA GAATCTCCAT CAGACATTCA TCTATGATGC AAATTCTCAT 
GAGGATGCAC ATGAAGAACT CCCATCCTCA GCCTTGCCAC TAACATCTCC GAGCCAGGGC 
TACAGAATAC AGGGCAGTAA ATTGACACAC AGTGTTTCAG ACAGTCTGTT TAACTGGGAG 
CCACATAGGA AGTACTTTGC AGAAGGTACA GCCTCAGTAT ATACCAGAGA AATGCCTGAA 
AGGCTAGCTC GTTGTGCTAG CCACTGCCCC TTGGAGAGGA AGCTATGTCC CAGTATACAC 
CTGCCCTGGG TGCAAAGAGA CAGGGACCCA CACAGCCAAG TTCATGGCCA TGTGCCTCTG 
AACCTGCAGC TCTCAGATAC CGATGACAAT GAGACCCTAA ACGAGCTACA CATAGAGAGC 
AGTGATGAGA GAAGCCCTTC AGACCTGTCC TTGACTGGGG ACACCGACAA GTCCATGGAG 
AACCTAGAGG TGGGATTCAG AAAGTCTGAA GGTGGCTCTC GGGACATGGG AGAAGAAGCA 
TGTCCCACCC ACTTAGAGAC TAGCCCGAAG GCATTCAGTT TCATCAAGCA ACAAAGGGTT 
GTCAAGAGGA CCTCTTCGGA AGAATGTGTC ACGGTCATAT TTGATGCAGA AAATGGAGAG 
CCAGTGGAAT TCAGCTCTCA CCAGGCTGGA GTCGTCACTG TCACCAGGGA TGAAATTTCC 
ATCAGCTCAG CCCTTGCTGG GCCCTCAGAG GAACTCACTG AACTTTCACC TCAGGAAATT 
ACTCACTTCC AGGCAGGAGC TACTGCAAGA GACTGTCCTT TTCTGAAGAG ACCTGAAGAG 
GAGACTGCGA AAAACATTCC AAAAGATGAA AATCATATTC TCACAGCTCC GAATGACTTC 
TTCAGCTCCC AGACTATAAT AACTCAGAAT TCCCCAAGAC CAAAGCTCGC CAAACCCACA 
CAGGGGGTAG CCTGGCAGAG TAATTCTAGA CCTGTTGGAA ACATGGGTGT CTACACAAAG 
CAAGGTCTGA CAAAAATCCC TTCCAGAGGC AAGTCTTCAC CTCAGAAATC CAAACTTGTG 
GAGTCTATGC CCACCATGCT GGTCCCCTCC AGTGCCCTAG TGACTCCTGA AAAGACACCA 
GCATCCCCTA TGGGGAAACT CTCACAATGT AGAAAGGCAG AGAGCCCTGG TTCTCTCTGT 
GATGTACAGC CAGAATCACA CATTCCAAAA CACCCTGCCC AGGTTCCTCA TATCCCCAAA 
ATTTCCAATA AGAAGAACTG GGTTCAGTGC CCCAGGAGTC AGACTTCAGG GATCCAGTCA 
CGGCATCTCA TGGGGAGAAG TGACAGTAGT GAAATGAGGT CCAATGGCTG TGTCTCTGCG 
CCACAGACAA GAATGAGGTC TCCCTCCCCT CCAACCCCTC CAGGGAGATC AGTGTCACTC 
CTGGTTAGAC CCAGTTATGA TTACCCACCA CTACCATCAC CTGCCAAACC TGAAACTCAG 
GGCCCTGGAG ACGCTGTAAG TCCAGGGTTG AAATCCCCGC TGTTGAAAGG ATCCTCTGCT 
CCTCTTGTCT CTTCCAGTCC AGTGATGTCA GAAATACAGA AGAAACCATC TGTGGCCTCC 
AGAAAGTCTG CCTTCCCTCC AGCTCTTCCC TCAACCCAAG CGGTAGTGCA CACCAGATAC 
CCTGAACACA TAGCCTCCAG TCCATTTGCT GTGACGACTT CAGGGCCCCC AAAAGTCTCT 
CCAAAAAGAG GCATCCCTAA ACCTCCCCCT CACCATGCAC TAGGAACTGC CCACATGGAC 
ACAGACTTAC AGACACCCAA GACTTGTCCT TCAAGCTGTG AACTGCTAGA GGTGACATCT 
TGTAAAAGCC TGTCTCCAGG GAGAAAAAGA CAGTTGAGTG ACAGCACTGT GATGCCCCAC 
AGGCCTTCCT TCTCAGGAGT GAATGAGTCC TTGACACCTC AGGTTAACAG TCCATCCTCC 
TCTTCCTCAT CCTGTAGAAA CCAAGGGTCC TCACAAGGTG GCCAGAACTC TCCCGAGAAA 
GGACCTAAAA CTCGCCTCCC AGTAGGACTC AAAGTTCTCA TGAAGTCTCC TCAGTTGCTC 
AAGAAAACTT CCTTGGTACC AGGGAAACAA GAAAAGGATA GTTTAAATGA AGCATCTCGA 
AGCTGTGTAG CAGCAAACAC GTCTAATCCT GAGGACTCCA AAGACCCTGC AAGTCTGGAG 
ACCTCAAGAG ATGAGAGAAC CACAGCCTTA CTAGGTGTGA GAGGTCCCGA CAGTCTAGCT 
AGAGGACTTG CCTTAGACTC AACAATGCCA GAATCGTTAG AATGTAGCAT CTCCAGAGAT 
GATGGAAGAG ATGGGGCAGA CAGCAGAGCC ATAAAAAGAT CCTTCTCCTC CAGCAAGCCA 
CACCTAAAGC CTGCTCTAGG CATGAATGGA GCCAAAGCTC GGAGTCAGAG CTTCAGCATC 
CACTTGGGAG AGAAGCCTTC CACACCCCCA ACAGAAGGGC CAGGCAAGAT TCGAACTCAG 
ATTATCACCA ACACGGCTGA AAGAGGCAAC TCCCTCACCA GACAGAATAT CTCAATGGAA 
GGGTCCCCAA GCAAGACCCC CTCTGTCTCA ACATCTGATA ATCTGCCCAA TGCTGGAAGG 
CCCCTGGGAA ACCCCTTTTC CAGGCAGAGT TCTCTCTGTA GCACCGGAAG TTCTAGCAGC 
CAGCACGGAA GCCCAAGCAA GTTACCCTTG AGGATGCTCA CAAGGCCGGA AGGACTCCTC 
ACTCCTGGAG TGGAAGACCA ACGGCTCTCT ACTCAGGAAG AGTGTCCTGA AAGAGGTGTA 
CATGAGCAAT CTAGCAGTGA CCACCATAGG CATCCATCTC CTCTTTCAGA CTGCCCTCAG 
TTCCTGCACG TTCCAGGGAG GATGCAGCGC CCAAGCAATT TGGAAACAAG TGGGATATCT 
AAGCTAGAAA CTTCTGGAAG GCATCCAGAT GCTTCTTCAA CTGGGACTAG TGTCGTGAGC 
CCAGAAGCCC CCCTCTCACC CTCCATCGAA GAAAAGGTCA TGTTGTGCAT TCAGGAGAAT 
GTGGAAAAGG GCCAAGTGCA GACAAAGTCT GCCTCTGTGG AAGTGAAGCC CAGGGCAGGG 
CCTTCGTTCA CCAGCTGGTT TGGTTTTCGA AAGAGCAGGC TTCCAGCTCT CAGTAGCAGG 
AGAATGGAAG CTTCCAAGAG TAAAGTGGAA AAGAAAGACA CAAAACTGAA ATCTGAGAGG 
AAAAAAGAAA AGAAGAAAGC TGAAGTACAA TCAAAGATAG AAAACGAACT GAGTAGGGGG 
ACCAAGAAGG CAGATGGTCA GAGTCCAGAC GATGGTCTGC AAAGCTCAGA GAGTCTGAAA 
GTATCCCAAG ACATTTATAA CCAAATGAAG TTGGAGCCAA GGAATAGACC CAGCCCTGCT 
GTGTGCCCAA CAAAAGATGC CTTCATGACA GAACTTCTAA ACAGAGTTGA TAAGAGAGCC 
TCGGTACAGA GAGAGAGTGG ATCAAAGGGT GTTTCCTGCA GGAGCGTGTT AGAGGGCACC 
TCCCAGGGCT CCTGTTTCGC CAGCGGCTCT GTGAGCACTC AAGGAAGTCA AAAGAAAAAC 
ATCAAAACCA AAGTCGATAT GGAAAAACCA AGAGAATCCT TGGGGGCCGA GGTCAATGAA 
GATGTACAAG AAGATGAAGA AGATAGAGTT GCTGACACTA CTCTCCAGAG CCACATGATA 
GAATCCAACT GCCAGATGAG AACACTGGAC AGCGGGATTG GAACCTTTCC CCTCCCAGAC 
TCAGGAAACC GCTCAGTGGG ACGATACATA TGCCAGCAGG ACTCTCCAGA GGACACTGAT 
GCCCTCCTGC CTCTCCAGCC AGCCGCTTCC ATGGCATCCT CTGCCAGAGC CCAAACCCTT 
GACCGCGAAG TGCCTTCCTC CACAGACAGC CAGAGCTCCC CGGAGTCCAT GATCATCCAT 
TCCATATCTG ACCCCAGCAT GATGGCCAGA GAGATGAGAG CCTCACAGAG TCACCTTCCC 
AAGCCTACCT CCTCAGGAAA ACCTAGTTTT GAAAAGCAAA ATGGAGCAGA GTCAAAGGCT 
CACACTGGCC CATCATTCGA ATATGCTGAG GATGCAGTGG CAAGTGAGCC CCTTTCAGGA 
TGGAGGGGTG AAGATGCTAC TACTGAGACC CAGAACAGGT GCCCTGAGGA AGCAGAAATC 
CTTCACAGAC TTGAAAGTGA CTCGAGGACA GTTAATCGTC ACCGTGCTCT AGTGAGGCAG 
GAACCGAAAC AAGTTGAAGA AACAAAAGAA GATCCTGAGA ATAGATTATC TGAAATTTCC 
CTGGAGTCAT TCAATAAATT TAGCAGCAAT ACTGTGATTT TATTAGGAAA AGAGAAGTCT 
CCAAGTGAGG TTGAAGGACA AAAGGAAGAG AAAGGCAAAA AGACAGAAGT GTCTTCGAGT 
AGCTCCGAAC GGCCTGGGGT GGACCGCGTG GAGTCTCTGA GTGACTCTCT GTATGACAGT 
TTCTCCTCTT GTGCAAGTCA AGCATCCAAC GAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246265.1
CDS	849..6767
Translation	MEGKRQLDRRDFGKRLSLDSSLVEYMDSNKCIEHLLAQLREQHRSLWREKLAVARLQREVAQRTSQGAMHEKLIHELEEERHLRLQSEERLREVTLESERNRIQMLGLQQQFSRMEETVRSLLQSQGSPEQKKEEPAKITAYQEGLPDEERKEQAALGDLHVVAADEDSRSEHSSVEEGKEDSRLLLKRLKALEAENSALALENENQREQYERCLDEVANQVVRALLTQKDLREECVKLKTRVFDLEQQNRALSLLFQQRVRPASDVLLQKRHSHVMALSSGDLLSEAGGSQSLALSWTDIELHEHLQNAKSGTPALKHSGSGVVIPDHLCPRNSCSSGSELSLSSTCSEYSSGSSYTWQDRKLIGKRQSSRIWDKRLSIDSSLPSGFASPADELPPTRIKESHILEGLRKLQKRKVFLESPSLVISKWGYKDCMNSNEGIYSPGIKTSSLKECPPCQPSGMGIPCQNLHQTFIYDANSHEDAHEELPSSALPLTSPSQGYRIQGSKLTHSVSDSLFNWEPHRKYFAEGTASVYTREMPERLARCASHCPLERKLCPSIHLPWVQRDRDPHSQVHGHVPLNLQLSDTDDNETLNELHIESSDERSPSDLSLTGDTDKSMENLEVGFRKSEGGSRDMGEEACPTHLETSPKAFSFIKQQRVVKRTSSEECVTVIFDAENGEPVEFSSHQAGVVTVTRDEISISSALAGPSEELTELSPQEITHFQAGATARDCPFLKRPEEETAKNIPKDENHILTAPNDFFSSQTIITQNSPRPKLAKPTQGVAWQSNSRPVGNMGVYTKQGLTKIPSRGKSSPQKSKLVESMPTMLVPSSALVTPEKTPASPMGKLSQCRKAESPGSLCDVQPESHIPKHPAQVPHIPKISNKKNWVQCPRSQTSGIQSRHLMGRSDSSEMRSNGCVSAPQTRMRSPSPPTPPGRSVSLLVRPSYDYPPLPSPAKPETQGPGDAVSPGLKSPLLKGSSAPLVSSSPVMSEIQKKPSVASRKSAFPPALPSTQAVVHTRYPEHIASSPFAVTTSGPPKVSPKRGIPKPPPHHALGTAHMDTDLQTPKTCPSSCELLEVTSCKSLSPGRKRQLSDSTVMPHRPSFSGVNESLTPQVNSPSSSSSSCRNQGSSQGGQNSPEKGPKTRLPVGLKVLMKSPQLLKKTSLVPGKQEKDSLNEASRSCVAANTSNPEDSKDPASLETSRDERTTALLGVRGPDSLARGLALDSTMPESLECSISRDDGRDGADSRAIKRSFSSSKPHLKPALGMNGAKARSQSFSIHLGEKPSTPPTEGPGKIRTQIITNTAERGNSLTRQNISMEGSPSKTPSVSTSDNLPNAGRPLGNPFSRQSSLCSTGSSSSQHGSPSKLPLRMLTRPEGLLTPGVEDQRLSTQEECPERGVHEQSSSDHHRHPSPLSDCPQFLHVPGRMQRPSNLETSGISKLETSGRHPDASSTGTSVVSPEAPLSPSIEEKVMLCIQENVEKGQVQTKSASVEVKPRAGPSFTSWFGFRKSRLPALSSRRMEASKSKVEKKDTKLKSERKKEKKKAEVQSKIENELSRGTKKADGQSPDDGLQSSESLKVSQDIYNQMKLEPRNRPSPAVCPTKDAFMTELLNRVDKRASVQRESGSKGVSCRSVLEGTSQGSCFASGSVSTQGSQKKNIKTKVDMEKPRESLGAEVNEDVQEDEEDRVADTTLQSHMIESNCQMRTLDSGIGTFPLPDSGNRSVGRYICQQDSPEDTDALLPLQPAASMASSARAQTLDREVPSSTDSQSSPESMIIHSISDPSMMAREMRASQSHLPKPTSSGKPSFEKQNGAESKAHTGPSFEYAEDAVASEPLSGWRGEDATTETQNRCPEEAEILHRLESDSRTVNRHRALVRQEPKQVEETKEDPENRLSEISLESFNKFSSNTVILLGKEKSPSEVEGQKEEKGKKTEVSSSSSERPGVDRVESLSDSLYDSFSSCASQASNEA

Target ORF information:

RefSeq Version	XM_011247963.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 5 (Nckap5), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247963.2

ORF Insert Sequence:

ATGGAGGGGA AGAGACAGCT GGATAGAAGG GACTTTGGTA AAAGACTCTC TCTGGACAGC 
AGTCTTGTGG AATACATGGA CTCTAACAAA TGCATCGAGC ACCTGCTGGC ACAACTCAGG 
GAGCAACACA GAAGTCTCTG GCGGGAGAAG CTGGCTGTGG CCAGGCTGCA GAGAGAAGTT 
GCCCAAAGGA CAAGCCAAGG GGCCATGCAC GAGAAGCTGA TACATGAGTT GGAAGAGGAA 
CGGCACTTGC GGCTACAGAG TGAGGAGCGG CTGCGGGAGG TGACCCTGGA GTCAGAGCGC 
AACAGGATCC AGATGCTCGG CCTGCAGCAG CAGTTTTCCA GAATGGAAGA AACTGTCCGA 
AGTCTGTTGC AGAGCCAAGG ATCTCCAGAG CAGAAAAAGG AAGAACCTGC CAAGATCACA 
GCCTACCAGG AAGGACTACC TGATGAAGAG AGAAAAGAGC AAGCAGCACT GGGTGATCTC 
CATGTGGTGG CAGCTGATGA GGACTCAAGA AGCGAGCACA GTAGTGTGGA GGAGGGAAAG 
GAAGACAGCA GACTGCTCCT GAAGAGACTG AAGGCCCTGG AGGCGGAGAA TTCAGCACTG 
GCACTGGAAA ACGAGAATCA GAGAGAACAG TATGAGCGAT GTCTTGATGA GGTAGCCAAC 
CAAGTTGTTC GAGCTCTACT CACTCAAAAG GATCTGAGGG AGGAGTGTGT AAAGTTGAAA 
ACAAGAGTGT TTGACTTGGA GCAACAGAAC CGAGCACTCA GCCTCCTGTT CCAACAGCGA 
GTCAGACCAG CTTCTGATGT GCTTCTCCAG AAACGCCACT CACATGTCAT GGCTCTCTCA 
TCTGGTGATC TGCTCTCAGA GGCAGGAGGA AGCCAAAGTC TGGCACTGTC GTGGACAGAT 
ATTGAGCTGC ATGAACACCT CCAGAATGCA AAATCAGGAA CCCCTGCCTT GAAACACTCT 
GGCTCAGGGG TTGTCATCCC TGATCACCTC TGTCCTCGCA ACAGCTGCAG CAGTGGCAGT 
GAGCTATCGC TGTCAAGCAC CTGCAGCGAG TACTCCAGTG GCTCCTCCTA CACGTGGCAA 
GACAGGAAGC TCATTGGGAA AAGGCAATCA TCGCGGATCT GGGATAAAAG ACTAAGTATT 
GATTCTTCGC TCCCAAGTGG CTTCGCCAGC CCTGCAGATG AACTACCTCC GACCCGTATC 
AAAGAAAGCC ACATTCTAGA GGGACTGAGG AAGCTACAGA AGCGGAAAGT GTTCCTTGAA 
TCACCATCAT TGGTGATAAG CAAATGGGGT TATAAAGATT GCATGAACTC GAATGAAGGG 
ATCTATTCGC CAGGAATTAA AACCAGCAGC CTCAAGGAGT GTCCGCCCTG CCAACCGTCT 
GGCATGGGGA TCCCCTGCCA GAATCTCCAT CAGACATTCA TCTATGATGC AAATTCTCAT 
GAGGATGCAC ATGAAGAACT CCCATCCTCA GCCTTGCCAC TAACATCTCC GAGCCAGGGC 
TACAGAATAC AGGGCAGTAA ATTGACACAC AGTGTTTCAG ACAGTCTGTT TAACTGGGAG 
CCACATAGGA AGTACTTTGC AGAAGGTACA GCCTCAGTAT ATACCAGAGA AATGCCTGAA 
AGGCTAGCTC GTTGTGCTAG CCACTGCCCC TTGGAGAGGA AGCTATGTCC CAGTATACAC 
CTGCCCTGGG TGCAAAGAGA CAGGGACCCA CACAGCCAAG TTCATGGCCA TGTGCCTCTG 
AACCTGCAGC TCTCAGATAC CGATGACAAT GAGACCCTAA ACGAGCTACA CATAGAGAGC 
AGTGATGAGA GAAGCCCTTC AGACCTGTCC TTGACTGGGG ACACCGACAA GTCCATGGAG 
AACCTAGAGG TGGGATTCAG AAAGTCTGAA GGTGGCTCTC GGGACATGGG AGAAGAAGCA 
TGTCCCACCC ACTTAGAGAC TAGCCCGAAG GCATTCAGTT TCATCAAGCA ACAAAGGGTT 
GTCAAGAGGA CCTCTTCGGA AGAATGTGTC ACGGTCATAT TTGATGCAGA AAATGGAGAG 
CCAGTGGAAT TCAGCTCTCA CCAGGCTGGA GTCGTCACTG TCACCAGGGA TGAAATTTCC 
ATCAGCTCAG CCCTTGCTGG GCCCTCAGAG GAACTCACTG AACTTTCACC TCAGGAAATT 
ACTCACTTCC AGGCAGGAGC TACTGCAAGA GACTGTCCTT TTCTGAAGAG ACCTGAAGAG 
GAGACTGCGA AAAACATTCC AAAAGATGAA AATCATATTC TCACAGCTCC GAATGACTTC 
TTCAGCTCCC AGACTATAAT AACTCAGAAT TCCCCAAGAC CAAAGCTCGC CAAACCCACA 
CAGGGGGTAG CCTGGCAGAG TAATTCTAGA CCTGTTGGAA ACATGGGTGT CTACACAAAG 
CAAGGTCTGA CAAAAATCCC TTCCAGAGGC AAGTCTTCAC CTCAGAAATC CAAACTTGTG 
GAGTCTATGC CCACCATGCT GGTCCCCTCC AGTGCCCTAG TGACTCCTGA AAAGACACCA 
GCATCCCCTA TGGGGAAACT CTCACAATGT AGAAAGGCAG AGAGCCCTGG TTCTCTCTGT 
GATGTACAGC CAGAATCACA CATTCCAAAA CACCCTGCCC AGGTTCCTCA TATCCCCAAA 
ATTTCCAATA AGAAGAACTG GGTTCAGTGC CCCAGGAGTC AGACTTCAGG GATCCAGTCA 
CGGCATCTCA TGGGGAGAAG TGACAGTAGT GAAATGAGGT CCAATGGCTG TGTCTCTGCG 
CCACAGACAA GAATGAGGTC TCCCTCCCCT CCAACCCCTC CAGGGAGATC AGTGTCACTC 
CTGGTTAGAC CCAGTTATGA TTACCCACCA CTACCATCAC CTGCCAAACC TGAAACTCAG 
GGCCCTGGAG ACGCTGTAAG TCCAGGGTTG AAATCCCCGC TGTTGAAAGG ATCCTCTGCT 
CCTCTTGTCT CTTCCAGTCC AGTGATGTCA GAAATACAGA AGAAACCATC TGTGGCCTCC 
AGAAAGTCTG CCTTCCCTCC AGCTCTTCCC TCAACCCAAG CGGTAGTGCA CACCAGATAC 
CCTGAACACA TAGCCTCCAG TCCATTTGCT GTGACGACTT CAGGGCCCCC AAAAGTCTCT 
CCAAAAAGAG GCATCCCTAA ACCTCCCCCT CACCATGCAC TAGGAACTGC CCACATGGAC 
ACAGACTTAC AGACACCCAA GACTTGTCCT TCAAGCTGTG AACTGCTAGA GGTGACATCT 
TGTAAAAGCC TGTCTCCAGG GAGAAAAAGA CAGTTGAGTG ACAGCACTGT GATGCCCCAC 
AGGCCTTCCT TCTCAGGAGT GAATGAGTCC TTGACACCTC AGGTTAACAG TCCATCCTCC 
TCTTCCTCAT CCTGTAGAAA CCAAGGGTCC TCACAAGGTG GCCAGAACTC TCCCGAGAAA 
GGACCTAAAA CTCGCCTCCC AGTAGGACTC AAAGTTCTCA TGAAGTCTCC TCAGTTGCTC 
AAGAAAACTT CCTTGGTACC AGGGAAACAA GAAAAGGATA GTTTAAATGA AGCATCTCGA 
AGCTGTGTAG CAGCAAACAC GTCTAATCCT GAGGACTCCA AAGACCCTGC AAGTCTGGAG 
ACCTCAAGAG ATGAGAGAAC CACAGCCTTA CTAGGTGTGA GAGGTCCCGA CAGTCTAGCT 
AGAGGACTTG CCTTAGACTC AACAATGCCA GAATCGTTAG AATGTAGCAT CTCCAGAGAT 
GATGGAAGAG ATGGGGCAGA CAGCAGAGCC ATAAAAAGAT CCTTCTCCTC CAGCAAGCCA 
CACCTAAAGC CTGCTCTAGG CATGAATGGA GCCAAAGCTC GGAGTCAGAG CTTCAGCATC 
CACTTGGGAG AGAAGCCTTC CACACCCCCA ACAGAAGGGC CAGGCAAGAT TCGAACTCAG 
ATTATCACCA ACACGGCTGA AAGAGGCAAC TCCCTCACCA GACAGAATAT CTCAATGGAA 
GGGTCCCCAA GCAAGACCCC CTCTGTCTCA ACATCTGATA ATCTGCCCAA TGCTGGAAGG 
CCCCTGGGAA ACCCCTTTTC CAGGCAGAGT TCTCTCTGTA GCACCGGAAG TTCTAGCAGC 
CAGCACGGAA GCCCAAGCAA GTTACCCTTG AGGATGCTCA CAAGGCCGGA AGGACTCCTC 
ACTCCTGGAG TGGAAGACCA ACGGCTCTCT ACTCAGGAAG AGTGTCCTGA AAGAGGTGTA 
CATGAGCAAT CTAGCAGTGA CCACCATAGG CATCCATCTC CTCTTTCAGA CTGCCCTCAG 
TTCCTGCACG TTCCAGGGAG GATGCAGCGC CCAAGCAATT TGGAAACAAG TGGGATATCT 
AAGCTAGAAA CTTCTGGAAG GCATCCAGAT GCTTCTTCAA CTGGGACTAG TGTCGTGAGC 
CCAGAAGCCC CCCTCTCACC CTCCATCGAA GAAAAGGTCA TGTTGTGCAT TCAGGAGAAT 
GTGGAAAAGG GCCAAGTGCA GACAAAGTCT GCCTCTGTGG AAGTGAAGCC CAGGGCAGGG 
CCTTCGTTCA CCAGCTGGTT TGGTTTTCGA AAGAGCAGGC TTCCAGCTCT CAGTAGCAGG 
AGAATGGAAG CTTCCAAGAG TAAAGTGGAA AAGAAAGACA CAAAACTGAA ATCTGAGAGG 
AAAAAAGAAA AGAAGAAAGC TGAAGTACAA TCAAAGATAG AAAACGAACT GAGTAGGGGG 
ACCAAGAAGG CAGATGGTCA GAGTCCAGAC GATGGTCTGC AAAGCTCAGA GAGTCTGAAA 
GTATCCCAAG ACATTTATAA CCAAATGAAG TTGGAGCCAA GGAATAGACC CAGCCCTGCT 
GTGTGCCCAA CAAAAGATGC CTTCATGACA GAACTTCTAA ACAGAGTTGA TAAGAGAGCC 
TCGGTACAGA GAGAGAGTGG ATCAAAGGGT GTTTCCTGCA GGAGCGTGTT AGAGGGCACC 
TCCCAGGGCT CCTGTTTCGC CAGCGGCTCT GTGAGCACTC AAGGAAGTCA AAAGAAAAAC 
ATCAAAACCA AAGTCGATAT GGAAAAACCA AGAGAATCCT TGGGGGCCGA GGTCAATGAA 
GATGTACAAG AAGATGAAGA AGATAGAGTT GCTGACACTA CTCTCCAGAG CCACATGATA 
GAATCCAACT GCCAGATGAG AACACTGGAC AGCGGGATTG GAACCTTTCC CCTCCCAGAC 
TCAGGAAACC GCTCAGTGGG ACGATACATA TGCCAGCAGG ACTCTCCAGA GGACACTGAT 
GCCCTCCTGC CTCTCCAGCC AGCCGCTTCC ATGGCATCCT CTGCCAGAGC CCAAACCCTT 
GACCGCGAAG TGCCTTCCTC CACAGACAGC CAGAGCTCCC CGGAGTCCAT GATCATCCAT 
TCCATATCTG ACCCCAGCAT GATGGCCAGA GAGATGAGAG CCTCACAGAG TCACCTTCCC 
AAGCCTACCT CCTCAGGAAA ACCTAGTTTT GAAAAGCAAA ATGGAGCAGA GTCAAAGGCT 
CACACTGGCC CATCATTCGA ATATGCTGAG GATGCAGTGG CAAGTGAGCC CCTTTCAGGA 
TGGAGGGGTG AAGATGCTAC TACTGAGACC CAGAACAGGT GCCCTGAGGA AGCAGAAATC 
CTTCACAGAC TTGAAAGTGA CTCGAGGACA GTTAATCGTC ACCGTGCTCT AGTGAGGCAG 
GAACCGAAAC AAGTTGAAGA AACAAAAGAA GATCCTGAGA ATAGATTATC TGAAATTTCC 
CTGGAGTCAT TCAATAAATT TAGCAGCAAT ACTGTGATTT TATTAGGAAA AGAGAAGTCT 
CCAAGTGAGG TTGAAGGACA AAAGGAAGAG AAAGGCAAAA AGACAGAAGT GTCTTCGAGT 
AGCTCCGAAC GGCCTGGGGT GGACCGCGTG GAGTCTCTGA GTGACTCTCT GTATGACAGT 
TTCTCCTCTT GTGCAAGTCA AGCATCCAAC GAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Nckap5
Entrez Gene ID	210356
Full Name	NCK-associated protein 5
Synonyms	8430408F21,D130011D22Rik,E030049G20Rik,Erih1,Erih2,Gm1548,Nap5
General protein information	Preferred Names NCK-associated protein 5 Names nck-associated protein 5 peripheral clock protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 E3

Homo sapiens (human)	NCKAP5	NP_997246.2
Canis lupus familiaris (dog)	NCKAP5	XP_005631978.1
Rattus norvegicus (Norway rat)	Nckap5	XP_344135.4
Gallus gallus (chicken)	NCKAP5	XP_422129.4
Bos taurus (cattle)	NCKAP5	XP_002685464.2
Pan troglodytes (chimpanzee)	NCKAP5	XP_001151197.2
	NCKAP5	XP_001102812.2
Mus musculus (house mouse)	Nckap5	NP_001075225.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Nckap5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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