TRIB2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRIB2
Entrez Gene ID	28951
Full Name	tribbles pseudokinase 2
Synonyms	C5FW,GS3955,TRB2
General protein information	Preferred Names tribbles pseudokinase 2 Names tribbles homolog 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p24.3
Summary	This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009].
Disorder MIM:	609462

mRNA and Protein(s)

mRNA	Protein	Name
NM_021643.3	NP_067675.1	tribbles homolog 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TRIB2	NP_001003218.1
Xenopus tropicalis (tropical clawed frog)	trib2	NP_989080.1
Homo sapiens (human)	TRIB2	NP_067675.1
Bos taurus (cattle)	TRIB2	NP_847887.2
Mus musculus (house mouse)	Trib2	NP_653134.2
Pan troglodytes (chimpanzee)	TRIB2	XP_515306.1
Gallus gallus (chicken)	TRIB2	NP_989732.2
Drosophila melanogaster (fruit fly)	trbl	NP_524672.1
Danio rerio (zebrafish)	trib2	XP_002667789.1

Gene Ontology
Bibliography

Related articles in PubMed

Trib2 regulates the pluripotency of embryonic stem cells and enhances reprogramming efficiency.
Do EK, Park JK, Cheon HC, Kwon YW, Heo SC, Choi EJ, Seo JK, Jang IH, Lee SC, Kim JH
Experimental & molecular medicine49(11)e401(2017 Nov)

Tribbles 2 mediates cisplatin sensitivity and DNA damage response in epithelial ovarian cancer.
Kritsch D, Hoffmann F, Steinbach D, Jansen L, Mary Photini S, Gajda M, Mosig AS, Sonnemann J, Peters S, Melnikova M, Thomale J, D?rst M, Runnebaum IB, H?fner N
International journal of cancer141(8)1600-1614(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tumors from resistant patients expressed the lowest levels of TRIB2. Downregulation of TRIB2 contributes to platin-resistance.
Title: Tribbles 2 mediates cisplatin sensitivity and DNA damage response in epithelial ovarian cancer.

Studied effect of TRIB2 SNP on the expression of genes involved in adaptive thermogenesis using messenger RNAs prepared from adipose tissues of Japanese adults. Of the five thermogenic genes, DIO2, CIDEA, PPARGC1A, and PRDM16 showed significantly higher transcript levels in SAT of individuals with the AA genotype relative to those with the AT + TT genotype.
Title: An adaptive variant of TRIB2, rs1057001, is associated with higher expression levels of thermogenic genes in human subcutaneous and visceral adipose tissues.

miR-206 and miR-140, as tumor suppressors, induced lung adenocarcinoma cell death and inhibited cell proliferation by modifying oncogenic TRIB2 promoter activity through p-Smad3.
Title: Smad3-related miRNAs regulated oncogenic TRIB2 promoter activity to effectively suppress lung adenocarcinoma growth.

O-GlcNAcylation of TRIB2 might be critical for diabetes-associated liver cancer.
Title: Reciprocal regulation between O-GlcNAcylation and tribbles pseudokinase 2 (TRIB2) maintains transformative phenotypes in liver cancer cells.

Data show that TRIB2-mediated degradation of CDC25C is associated with lysine-48-linked CDC25C polyubiquitination driven by the TRIB2 kinase-like domain.
Title: Human TRIB2 Oscillates during the Cell Cycle and Promotes Ubiquitination and Degradation of CDC25C.

The following TRIB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu19007	NM_021643.3 Latest version!	Homo sapiens tribbles pseudokinase 2 (TRIB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRIB2 ( NM_021643.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_067675.1 Homo sapiens tribbles pseudokinase 2 (TRIB2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19007
Clone ID Related Accession (Same CDS sequence)	NM_021643.3
Accession Version	NM_021643.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	tribbles homolog 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC379987.1, AY245544.1 and AC009486.4. This sequence is a reference standard in the RefSeqGene project. On May 7, 2008 this sequence version replaced NM_021643.2. Transcript Variant: This variant (1) encodes a functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D87119.1, SRR1660807.14020.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACATAC ACAGGTCTAC CCCCATCACA ATAGCGAGAT ATGGGAGATC GCGGAACAAA 
ACCCAGGATT TCGAAGAGTT GTCGTCTATA AGGTCCGCGG AGCCCAGCCA GAGTTTCAGC 
CCGAACCTCG GCTCCCCGAG CCCGCCCGAG ACTCCGAACT TGTCGCATTG CGTTTCTTGT 
ATCGGGAAAT ACTTATTGTT GGAACCTCTG GAGGGAGACC ACGTTTTTCG TGCCGTGCAT 
CTGCACAGCG GAGAGGAGCT GGTGTGCAAG GTGTTTGATA TCAGCTGCTA CCAGGAATCC 
CTGGCACCGT GCTTTTGCCT GTCTGCTCAT AGTAACATCA ACCAAATCAC TGAAATTATC 
CTGGGTGAGA CCAAAGCCTA TGTGTTCTTT GAGCGAAGCT ATGGGGACAT GCATTCCTTC 
GTCCGCACCT GCAAGAAGCT GAGAGAGGAG GAGGCAGCCA GACTGTTCTA CCAGATTGCC 
TCGGCAGTGG CCCACTGCCA TGACGGGGGG CTGGTGCTGC GGGACCTCAA GCTGCGGAAA 
TTCATCTTTA AGGACGAAGA GAGGACTCGG GTCAAGCTGG AAAGCCTGGA AGACGCCTAC 
ATTCTGCGGG GAGATGATGA TTCCCTCTCC GACAAGCATG GCTGCCCGGC TTACGTAAGC 
CCAGAGATCT TGAACACCAG TGGCAGCTAC TCGGGCAAAG CAGCCGACGT GTGGAGCCTG 
GGGGTGATGC TGTACACCAT GTTGGTGGGG CGGTACCCTT TCCATGACAT TGAACCCAGC 
TCCCTCTTCA GCAAGATCCG GCGTGGCCAG TTCAACATTC CAGAGACTCT GTCGCCCAAG 
GCCAAGTGCC TCATCCGAAG CATTCTGCGT CGGGAGCCCT CAGAGCGGCT GACCTCGCAG 
GAAATTCTGG ACCATCCTTG GTTTTCTACA GATTTTAGCG TCTCGAATTC AGCATATGGT 
GCTAAGGAAG TGTCTGACCA GCTGGTGCCG GACGTCAACA TGGAAGAGAA CTTGGACCCT 
TTCTTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067675.1
CDS	1437..2468
Translation	MNIHRSTPITIARYGRSRNKTQDFEELSSIRSAEPSQSFSPNLGSPSPPETPNLSHCVSCIGKYLLLEPLEGDHVFRAVHLHSGEELVCKVFDISCYQESLAPCFCLSAHSNINQITEIILGETKAYVFFERSYGDMHSFVRTCKKLREEEAARLFYQIASAVAHCHDGGLVLRDLKLRKFIFKDEERTRVKLESLEDAYILRGDDDSLSDKHGCPAYVSPEILNTSGSYSGKAADVWSLGVMLYTMLVGRYPFHDIEPSSLFSKIRRGQFNIPETLSPKAKCLIRSILRREPSERLTSQEILDHPWFSTDFSVSNSAYGAKEVSDQLVPDVNMEENLDPFFN

Target ORF information:

RefSeq Version	NM_021643.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tribbles pseudokinase 2 (TRIB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021643.3

ORF Insert Sequence:

ATGAACATAC ACAGGTCTAC CCCCATCACA ATAGCGAGAT ATGGGAGATC GCGGAACAAA 
ACCCAGGATT TCGAAGAGTT GTCGTCTATA AGGTCCGCGG AGCCCAGCCA GAGTTTCAGC 
CCGAACCTCG GCTCCCCGAG CCCGCCCGAG ACTCCGAACT TGTCGCATTG CGTTTCTTGT 
ATCGGGAAAT ACTTATTGTT GGAACCTCTG GAGGGAGACC ACGTTTTTCG TGCCGTGCAT 
CTGCACAGCG GAGAGGAGCT GGTGTGCAAG GTGTTTGATA TCAGCTGCTA CCAGGAATCC 
CTGGCACCGT GCTTTTGCCT GTCTGCTCAT AGTAACATCA ACCAAATCAC TGAAATTATC 
CTGGGTGAGA CCAAAGCCTA TGTGTTCTTT GAGCGAAGCT ATGGGGACAT GCATTCCTTC 
GTCCGCACCT GCAAGAAGCT GAGAGAGGAG GAGGCAGCCA GACTGTTCTA CCAGATTGCC 
TCGGCAGTGG CCCACTGCCA TGACGGGGGG CTGGTGCTGC GGGACCTCAA GCTGCGGAAA 
TTCATCTTTA AGGACGAAGA GAGGACTCGG GTCAAGCTGG AAAGCCTGGA AGACGCCTAC 
ATTCTGCGGG GAGATGATGA TTCCCTCTCC GACAAGCATG GCTGCCCGGC TTACGTAAGC 
CCAGAGATCT TGAACACCAG TGGCAGCTAC TCGGGCAAAG CAGCCGACGT GTGGAGCCTG 
GGGGTGATGC TGTACACCAT GTTGGTGGGG CGGTACCCTT TCCATGACAT TGAACCCAGC 
TCCCTCTTCA GCAAGATCCG GCGTGGCCAG TTCAACATTC CAGAGACTCT GTCGCCCAAG 
GCCAAGTGCC TCATCCGAAG CATTCTGCGT CGGGAGCCCT CAGAGCGGCT GACCTCGCAG 
GAAATTCTGG ACCATCCTTG GTTTTCTACA GATTTTAGCG TCTCGAATTC AGCATATGGT 
GCTAAGGAAG TGTCTGACCA GCTGGTGCCG GACGTCAACA TGGAAGAGAA CTTGGACCCT 
TTCTTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Trib2 regulates the pluripotency of embryonic stem cells and enhances reprogramming efficiency.
Experimental & molecular medicine49(11)e401(2017 Nov)
Do EK,Park JK,Cheon HC,Kwon YW,Heo SC,Choi EJ,Seo JK,Jang IH,Lee SC,Kim JH

Tribbles 2 mediates cisplatin sensitivity and DNA damage response in epithelial ovarian cancer.
International journal of cancer141(8)1600-1614(2017 Oct)
Kritsch D,Hoffmann F,Steinbach D,Jansen L,Mary Photini S,Gajda M,Mosig AS,Sonnemann J,Peters S,Melnikova M,Thomale J,D?rst M,Runnebaum IB,H?fner N

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TRIB2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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