NWD1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001347994.1	NP_001334923.1	NACHT domain- and WD repeat-containing protein 1 isoform 3
XM_011527929.1	XP_011526231.1	NACHT domain- and WD repeat-containing protein 1 isoform X1
XM_024451467.1	XP_024307235.1	NACHT domain- and WD repeat-containing protein 1 isoform X4
XM_011527934.1	XP_011526236.1	NACHT domain- and WD repeat-containing protein 1 isoform X5
XM_011527935.1	XP_011526237.1	NACHT domain- and WD repeat-containing protein 1 isoform X6
NM_001290355.2	NP_001277284.1	NACHT domain- and WD repeat-containing protein 1 isoform 2
XM_011527931.2	XP_011526233.1	NACHT domain- and WD repeat-containing protein 1 isoform X1
XM_024451466.1	XP_024307234.1	NACHT domain- and WD repeat-containing protein 1 isoform X2
NM_001007525.4	NP_001007526.3	NACHT domain- and WD repeat-containing protein 1 isoform 1
XM_011527930.2	XP_011526232.1	NACHT domain- and WD repeat-containing protein 1 isoform X1
NM_001290355.3	NP_001277284.1	NACHT domain- and WD repeat-containing protein 1 isoform 2
NM_001007525.5	NP_001007526.3	NACHT domain- and WD repeat-containing protein 1 isoform 1
XM_017026669.1	XP_016882158.1	NACHT domain- and WD repeat-containing protein 1 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09300	NM_001007525.5 Latest version!	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu09300	XM_024451466.1 Latest version!	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu104660	XM_024451467.1 Latest version!	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu78812	XM_011527934.1 Latest version!	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu78813	XM_011527935.1 Latest version!	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu09297	NM_001290355.3 Latest version!	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu78811	NM_001347994.1 Latest version!	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu78810	XM_011527930.2 Latest version!	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu78810	XM_011527931.2 Latest version!	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu78810	XM_011527929.1 Latest version!	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu78811	XM_017026669.1 Latest version!	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu09297	NM_001290355.2	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu09300	NM_001007525.4	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
Hide Old Accession Versions >>

NWD1 ( NM_001007525.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001007526.3 Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09300
Clone ID Related Accession (Same CDS sequence)	XM_024451466.1 , NM_001007525.4 , NM_001007525.5
Accession Version	NM_001007525.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	NACHT domain- and WD repeat-containing protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX489211.1, CR749794.1, AC008737.11, BC117698.1, AK090685.1 and CB242932.1. This sequence is a reference standard in the RefSeqGene project. On Dec 29, 2016 this sequence version replaced NM_001007525.3. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: CR749794.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2153427 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGTTTCCT CCAAAGGGGA CAGATTGCTG 
GAGAAGCTTC CAGATGCTGT GAGGTTCCTG GTGGTCTCTG AAGATGAGTC CCTCCTCGCC 
GCAGGCTTTG GAAGATCGGT GCGGATATTC TTGGCGGACT CGAGGGGCTT TCGCCGATTC 
ATGGCCATGG ATCTGGAACA TGAAGACATG GTGGAGACGG CTGTTTTTGG TACTGAGAAC 
AACCTGATCA TCACGGGGTC CCTTGATGCG CTCATTCAGG TGTGGAGTCT GTCAGAACAG 
GGGACCCTTC TGGACATCCT GGAAGGCGTC GGGGCCCCCG TGAGCCTGCT GGCCCGCGGC 
GGGGCTTTGG TGGCATCTGC TTCCCCACAG TCCTCATCTT TCAAGGTCTG GGATCTCAGC 
GATGCTCATA GGTCCCGGGT GCCTGCACCA TTTCTGGACC GCACCGGCCT CACCGCAGTG 
TCCCACAATG GAAGCTACGT CTACTTCCCC AAAATTGGGG ACAAAAACAA AGTCACTATT 
TGGGACTTGG CAGAAGGCGA GGAACAAGAT TCCCTGGACA CCTCCAGTGA GATCAGGTGT 
CTGGAGGTTG CTGAGCAGCG CAAGCTCCTA TTTACGGGCC TCGTGTCGGG GGTCGTCCTT 
GTGTTCCCCC TGAATTCCAG GCAGGACGTG ATATGCATTC CCCCTCCCGA GGCCCGGAAA 
GCAATCAACT GCATGTCCCT GAGCAAGTGC GAGGACCGCC TGGCCATCGC CTATGACAAC 
ATCGTCCTGG TGCTGGACAT CACCTCCGGG GACCCCTGCC CGGTCATCGA TGGGCCAAGA 
TACACCTTTT ACACTCAGCT GCCCGAGACC CTCTCCAGCG TGGCCATTCT GACGGACTAC 
CGCGTGGTCT ACAGCATGAC CAATGGGGAC CTCTTTCTTT ACGAGTGTGC AACTTCCAAA 
GCGTTTCCCT TGGAGACCCA CAGGAGCCGA GTTGCCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGTGG TCAGCGGGTC TGAGGATGCC CTGCTGTGTC TCTGGGACCT GCAGGCACGC 
AAGTGGAAAT TCGAGATGAG CTACACGGCA CCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007526.3
CDS	423..4721
Translation	MQRGKPCRALPTLKCQTFCQRHGLMFEVVDLRWGIRNIEATDHLTTELCLEEVDRCWKTSIGPAFVALIGDQYGPCLIPSRIDEKEWEVLRDHLTARPSDLELVARYFQRDENAFPPTYVLQAPGTGEACEPEEATLTSVLRSGAQEARRLGLITQEQWQHYHRSVIEWEIERSLLSSEDREQGATVFLREIQDLHKHILEDCALRMVDRLADGCLDADAQNLLSSLKSHITDMHPGVLKTHRLPWSRDLVNPKNKTHACYLKELGEQFVVRANHQVLTRLRELDTAGQELAWLYQEIRHHLWQSSEVIQTFCGRQELLARLGQQLRHDDSKQHTPLVLFGPPGIGKTALMCKLAEQMPRLLGHKTVTVLRLLGTSQMSSDARGLLKSICFQVCLAYGLPLPPAQVLDAHTRVVQFFHTLLHTVSCRNFESLVLLLDAMDDLDSVRHARRVPWLPLNCPPRVHLILSACSGALGVLDTLQRVLLDPEAYWEVKPLSGNQGQQMIQLLLAAARRTLSPVHTDLLWASLPECGNPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCTRLEQTHGQLLVAHVLGYIVSSRHGLSEAELKDVLSLDDEVLQDVYRDWTPPSKELLRFPPLLWVRLRRDLGYYLARRPVDGFTLLAIAHRQLVEVVRERYLSGSERAKRHGVLADFFSGTWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSHTVANLRKLKELPYHLLHSGRLEELKQEVLGSMSWISCRGISGGIEDLLDDFDLCAPHLDSPEVGLVREALQLCRPAVELRGMERSLLYTELLARLHFFATSHPALVGQLCQQAQSWFQLCAHPVLVPLGGFLQPPGGPLRATLSGCHKGITAMAWGVEEKLLVIGTQDGIMAVWDMEEQHVIHMLTGHTGEVRCVKIFAKGTLANSASKDYTLHLWNLLSGQEKFTIWDGGSKNPAEPQIWNLHVDEAHKVVYSASGSKINAWNLETAEPVFHILGDASDPWMCMAVLASQATLLTVSRDGVVSLWSSATGKLQGKQHMSSIKEETPTCAVSVQKQGKLVTGFSNGSISLVSSKGDRLLEKLPDAVRFLVVSEDESLLAAGFGRSVRIFLADSRGFRRFMAMDLEHEDMVETAVFGTENNLIITGSLDALIQVWSLSEQGTLLDILEGVGAPVSLLARGGALVASASPQSSSFKVWDLSDAHRSRVPAPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDLAEGEEQDSLDTSSEIRCLEVAEQRKLLFTGLVSGVVLVFPLNSRQDVICIPPPEARKAINCMSLSKCEDRLAIAYDNIVLVLDITSGDPCPVIDGPRYTFYTQLPETLSSVAILTDYRVVYSMTNGDLFLYECATSKAFPLETHRSRVACVEVSHKEQLVVSGSEDALLCLWDLQARKWKFEMSYTAPC

Target ORF information:

RefSeq Version	NM_001007525.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007525.4

ORF Insert Sequence:

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGTTTCCT CCAAAGGGGA CAGATTGCTG 
GAGAAGCTTC CAGATGCTGT GAGGTTCCTG GTGGTCTCTG AAGATGAGTC CCTCCTCGCC 
GCAGGCTTTG GAAGATCGGT GCGGATATTC TTGGCGGACT CGAGGGGCTT TCGCCGATTC 
ATGGCCATGG ATCTGGAACA TGAAGACATG GTGGAGACGG CTGTTTTTGG TACTGAGAAC 
AACCTGATCA TCACGGGGTC CCTTGATGCG CTCATTCAGG TGTGGAGTCT GTCAGAACAG 
GGGACCCTTC TGGACATCCT GGAAGGCGTC GGGGCCCCCG TGAGCCTGCT GGCCCGCGGC 
GGGGCTTTGG TGGCATCTGC TTCCCCACAG TCCTCATCTT TCAAGGTCTG GGATCTCAGC 
GATGCTCATA GGTCCCGGGT GCCTGCACCA TTTCTGGACC GCACCGGCCT CACCGCAGTG 
TCCCACAATG GAAGCTACGT CTACTTCCCC AAAATTGGGG ACAAAAACAA AGTCACTATT 
TGGGACTTGG CAGAAGGCGA GGAACAAGAT TCCCTGGACA CCTCCAGTGA GATCAGGTGT 
CTGGAGGTTG CTGAGCAGCG CAAGCTCCTA TTTACGGGCC TCGTGTCGGG GGTCGTCCTT 
GTGTTCCCCC TGAATTCCAG GCAGGACGTG ATATGCATTC CCCCTCCCGA GGCCCGGAAA 
GCAATCAACT GCATGTCCCT GAGCAAGTGC GAGGACCGCC TGGCCATCGC CTATGACAAC 
ATCGTCCTGG TGCTGGACAT CACCTCCGGG GACCCCTGCC CGGTCATCGA TGGGCCAAGA 
TACACCTTTT ACACTCAGCT GCCCGAGACC CTCTCCAGCG TGGCCATTCT GACGGACTAC 
CGCGTGGTCT ACAGCATGAC CAATGGGGAC CTCTTTCTTT ACGAGTGTGC AACTTCCAAA 
GCGTTTCCCT TGGAGACCCA CAGGAGCCGA GTTGCCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGTGG TCAGCGGGTC TGAGGATGCC CTGCTGTGTC TCTGGGACCT GCAGGCACGC 
AAGTGGAAAT TCGAGATGAG CTACACGGCA CCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( NM_001007525.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001007526.3 Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09300
Clone ID Related Accession (Same CDS sequence)	XM_024451466.1 , NM_001007525.4 , NM_001007525.5
Accession Version	NM_001007525.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	NACHT domain- and WD repeat-containing protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008737.11, BX489211.1, CR749794.1, BC117698.1, AK090685.1 and CB242932.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001007525.4. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: CR749794.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2153427 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGTTTCCT CCAAAGGGGA CAGATTGCTG 
GAGAAGCTTC CAGATGCTGT GAGGTTCCTG GTGGTCTCTG AAGATGAGTC CCTCCTCGCC 
GCAGGCTTTG GAAGATCGGT GCGGATATTC TTGGCGGACT CGAGGGGCTT TCGCCGATTC 
ATGGCCATGG ATCTGGAACA TGAAGACATG GTGGAGACGG CTGTTTTTGG TACTGAGAAC 
AACCTGATCA TCACGGGGTC CCTTGATGCG CTCATTCAGG TGTGGAGTCT GTCAGAACAG 
GGGACCCTTC TGGACATCCT GGAAGGCGTC GGGGCCCCCG TGAGCCTGCT GGCCCGCGGC 
GGGGCTTTGG TGGCATCTGC TTCCCCACAG TCCTCATCTT TCAAGGTCTG GGATCTCAGC 
GATGCTCATA GGTCCCGGGT GCCTGCACCA TTTCTGGACC GCACCGGCCT CACCGCAGTG 
TCCCACAATG GAAGCTACGT CTACTTCCCC AAAATTGGGG ACAAAAACAA AGTCACTATT 
TGGGACTTGG CAGAAGGCGA GGAACAAGAT TCCCTGGACA CCTCCAGTGA GATCAGGTGT 
CTGGAGGTTG CTGAGCAGCG CAAGCTCCTA TTTACGGGCC TCGTGTCGGG GGTCGTCCTT 
GTGTTCCCCC TGAATTCCAG GCAGGACGTG ATATGCATTC CCCCTCCCGA GGCCCGGAAA 
GCAATCAACT GCATGTCCCT GAGCAAGTGC GAGGACCGCC TGGCCATCGC CTATGACAAC 
ATCGTCCTGG TGCTGGACAT CACCTCCGGG GACCCCTGCC CGGTCATCGA TGGGCCAAGA 
TACACCTTTT ACACTCAGCT GCCCGAGACC CTCTCCAGCG TGGCCATTCT GACGGACTAC 
CGCGTGGTCT ACAGCATGAC CAATGGGGAC CTCTTTCTTT ACGAGTGTGC AACTTCCAAA 
GCGTTTCCCT TGGAGACCCA CAGGAGCCGA GTTGCCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGTGG TCAGCGGGTC TGAGGATGCC CTGCTGTGTC TCTGGGACCT GCAGGCACGC 
AAGTGGAAAT TCGAGATGAG CTACACGGCA CCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007526.3
CDS	552..4850
Translation	MQRGKPCRALPTLKCQTFCQRHGLMFEVVDLRWGIRNIEATDHLTTELCLEEVDRCWKTSIGPAFVALIGDQYGPCLIPSRIDEKEWEVLRDHLTARPSDLELVARYFQRDENAFPPTYVLQAPGTGEACEPEEATLTSVLRSGAQEARRLGLITQEQWQHYHRSVIEWEIERSLLSSEDREQGATVFLREIQDLHKHILEDCALRMVDRLADGCLDADAQNLLSSLKSHITDMHPGVLKTHRLPWSRDLVNPKNKTHACYLKELGEQFVVRANHQVLTRLRELDTAGQELAWLYQEIRHHLWQSSEVIQTFCGRQELLARLGQQLRHDDSKQHTPLVLFGPPGIGKTALMCKLAEQMPRLLGHKTVTVLRLLGTSQMSSDARGLLKSICFQVCLAYGLPLPPAQVLDAHTRVVQFFHTLLHTVSCRNFESLVLLLDAMDDLDSVRHARRVPWLPLNCPPRVHLILSACSGALGVLDTLQRVLLDPEAYWEVKPLSGNQGQQMIQLLLAAARRTLSPVHTDLLWASLPECGNPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCTRLEQTHGQLLVAHVLGYIVSSRHGLSEAELKDVLSLDDEVLQDVYRDWTPPSKELLRFPPLLWVRLRRDLGYYLARRPVDGFTLLAIAHRQLVEVVRERYLSGSERAKRHGVLADFFSGTWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSHTVANLRKLKELPYHLLHSGRLEELKQEVLGSMSWISCRGISGGIEDLLDDFDLCAPHLDSPEVGLVREALQLCRPAVELRGMERSLLYTELLARLHFFATSHPALVGQLCQQAQSWFQLCAHPVLVPLGGFLQPPGGPLRATLSGCHKGITAMAWGVEEKLLVIGTQDGIMAVWDMEEQHVIHMLTGHTGEVRCVKIFAKGTLANSASKDYTLHLWNLLSGQEKFTIWDGGSKNPAEPQIWNLHVDEAHKVVYSASGSKINAWNLETAEPVFHILGDASDPWMCMAVLASQATLLTVSRDGVVSLWSSATGKLQGKQHMSSIKEETPTCAVSVQKQGKLVTGFSNGSISLVSSKGDRLLEKLPDAVRFLVVSEDESLLAAGFGRSVRIFLADSRGFRRFMAMDLEHEDMVETAVFGTENNLIITGSLDALIQVWSLSEQGTLLDILEGVGAPVSLLARGGALVASASPQSSSFKVWDLSDAHRSRVPAPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDLAEGEEQDSLDTSSEIRCLEVAEQRKLLFTGLVSGVVLVFPLNSRQDVICIPPPEARKAINCMSLSKCEDRLAIAYDNIVLVLDITSGDPCPVIDGPRYTFYTQLPETLSSVAILTDYRVVYSMTNGDLFLYECATSKAFPLETHRSRVACVEVSHKEQLVVSGSEDALLCLWDLQARKWKFEMSYTAPC

Target ORF information:

RefSeq Version	NM_001007525.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007525.5

ORF Insert Sequence:

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGTTTCCT CCAAAGGGGA CAGATTGCTG 
GAGAAGCTTC CAGATGCTGT GAGGTTCCTG GTGGTCTCTG AAGATGAGTC CCTCCTCGCC 
GCAGGCTTTG GAAGATCGGT GCGGATATTC TTGGCGGACT CGAGGGGCTT TCGCCGATTC 
ATGGCCATGG ATCTGGAACA TGAAGACATG GTGGAGACGG CTGTTTTTGG TACTGAGAAC 
AACCTGATCA TCACGGGGTC CCTTGATGCG CTCATTCAGG TGTGGAGTCT GTCAGAACAG 
GGGACCCTTC TGGACATCCT GGAAGGCGTC GGGGCCCCCG TGAGCCTGCT GGCCCGCGGC 
GGGGCTTTGG TGGCATCTGC TTCCCCACAG TCCTCATCTT TCAAGGTCTG GGATCTCAGC 
GATGCTCATA GGTCCCGGGT GCCTGCACCA TTTCTGGACC GCACCGGCCT CACCGCAGTG 
TCCCACAATG GAAGCTACGT CTACTTCCCC AAAATTGGGG ACAAAAACAA AGTCACTATT 
TGGGACTTGG CAGAAGGCGA GGAACAAGAT TCCCTGGACA CCTCCAGTGA GATCAGGTGT 
CTGGAGGTTG CTGAGCAGCG CAAGCTCCTA TTTACGGGCC TCGTGTCGGG GGTCGTCCTT 
GTGTTCCCCC TGAATTCCAG GCAGGACGTG ATATGCATTC CCCCTCCCGA GGCCCGGAAA 
GCAATCAACT GCATGTCCCT GAGCAAGTGC GAGGACCGCC TGGCCATCGC CTATGACAAC 
ATCGTCCTGG TGCTGGACAT CACCTCCGGG GACCCCTGCC CGGTCATCGA TGGGCCAAGA 
TACACCTTTT ACACTCAGCT GCCCGAGACC CTCTCCAGCG TGGCCATTCT GACGGACTAC 
CGCGTGGTCT ACAGCATGAC CAATGGGGAC CTCTTTCTTT ACGAGTGTGC AACTTCCAAA 
GCGTTTCCCT TGGAGACCCA CAGGAGCCGA GTTGCCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGTGG TCAGCGGGTC TGAGGATGCC CTGCTGTGTC TCTGGGACCT GCAGGCACGC 
AAGTGGAAAT TCGAGATGAG CTACACGGCA CCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_024451466.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307234.1 Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X4, mRNA.

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CloneID	OHu09300
Clone ID Related Accession (Same CDS sequence)	XM_024451466.1 , NM_001007525.4 , NM_001007525.5
Accession Version	XM_024451466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGTTTCCT CCAAAGGGGA CAGATTGCTG 
GAGAAGCTTC CAGATGCTGT GAGGTTCCTG GTGGTCTCTG AAGATGAGTC CCTCCTCGCC 
GCAGGCTTTG GAAGATCGGT GCGGATATTC TTGGCGGACT CGAGGGGCTT TCGCCGATTC 
ATGGCCATGG ATCTGGAACA TGAAGACATG GTGGAGACGG CTGTTTTTGG TACTGAGAAC 
AACCTGATCA TCACGGGGTC CCTTGATGCG CTCATTCAGG TGTGGAGTCT GTCAGAACAG 
GGGACCCTTC TGGACATCCT GGAAGGCGTC GGGGCCCCCG TGAGCCTGCT GGCCCGCGGC 
GGGGCTTTGG TGGCATCTGC TTCCCCACAG TCCTCATCTT TCAAGGTCTG GGATCTCAGC 
GATGCTCATA GGTCCCGGGT GCCTGCACCA TTTCTGGACC GCACCGGCCT CACCGCAGTG 
TCCCACAATG GAAGCTACGT CTACTTCCCC AAAATTGGGG ACAAAAACAA AGTCACTATT 
TGGGACTTGG CAGAAGGCGA GGAACAAGAT TCCCTGGACA CCTCCAGTGA GATCAGGTGT 
CTGGAGGTTG CTGAGCAGCG CAAGCTCCTA TTTACGGGCC TCGTGTCGGG GGTCGTCCTT 
GTGTTCCCCC TGAATTCCAG GCAGGACGTG ATATGCATTC CCCCTCCCGA GGCCCGGAAA 
GCAATCAACT GCATGTCCCT GAGCAAGTGC GAGGACCGCC TGGCCATCGC CTATGACAAC 
ATCGTCCTGG TGCTGGACAT CACCTCCGGG GACCCCTGCC CGGTCATCGA TGGGCCAAGA 
TACACCTTTT ACACTCAGCT GCCCGAGACC CTCTCCAGCG TGGCCATTCT GACGGACTAC 
CGCGTGGTCT ACAGCATGAC CAATGGGGAC CTCTTTCTTT ACGAGTGTGC AACTTCCAAA 
GCGTTTCCCT TGGAGACCCA CAGGAGCCGA GTTGCCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGTGG TCAGCGGGTC TGAGGATGCC CTGCTGTGTC TCTGGGACCT GCAGGCACGC 
AAGTGGAAAT TCGAGATGAG CTACACGGCA CCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307234.1
CDS	607..4905
Translation	MQRGKPCRALPTLKCQTFCQRHGLMFEVVDLRWGIRNIEATDHLTTELCLEEVDRCWKTSIGPAFVALIGDQYGPCLIPSRIDEKEWEVLRDHLTARPSDLELVARYFQRDENAFPPTYVLQAPGTGEACEPEEATLTSVLRSGAQEARRLGLITQEQWQHYHRSVIEWEIERSLLSSEDREQGATVFLREIQDLHKHILEDCALRMVDRLADGCLDADAQNLLSSLKSHITDMHPGVLKTHRLPWSRDLVNPKNKTHACYLKELGEQFVVRANHQVLTRLRELDTAGQELAWLYQEIRHHLWQSSEVIQTFCGRQELLARLGQQLRHDDSKQHTPLVLFGPPGIGKTALMCKLAEQMPRLLGHKTVTVLRLLGTSQMSSDARGLLKSICFQVCLAYGLPLPPAQVLDAHTRVVQFFHTLLHTVSCRNFESLVLLLDAMDDLDSVRHARRVPWLPLNCPPRVHLILSACSGALGVLDTLQRVLLDPEAYWEVKPLSGNQGQQMIQLLLAAARRTLSPVHTDLLWASLPECGNPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCTRLEQTHGQLLVAHVLGYIVSSRHGLSEAELKDVLSLDDEVLQDVYRDWTPPSKELLRFPPLLWVRLRRDLGYYLARRPVDGFTLLAIAHRQLVEVVRERYLSGSERAKRHGVLADFFSGTWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSHTVANLRKLKELPYHLLHSGRLEELKQEVLGSMSWISCRGISGGIEDLLDDFDLCAPHLDSPEVGLVREALQLCRPAVELRGMERSLLYTELLARLHFFATSHPALVGQLCQQAQSWFQLCAHPVLVPLGGFLQPPGGPLRATLSGCHKGITAMAWGVEEKLLVIGTQDGIMAVWDMEEQHVIHMLTGHTGEVRCVKIFAKGTLANSASKDYTLHLWNLLSGQEKFTIWDGGSKNPAEPQIWNLHVDEAHKVVYSASGSKINAWNLETAEPVFHILGDASDPWMCMAVLASQATLLTVSRDGVVSLWSSATGKLQGKQHMSSIKEETPTCAVSVQKQGKLVTGFSNGSISLVSSKGDRLLEKLPDAVRFLVVSEDESLLAAGFGRSVRIFLADSRGFRRFMAMDLEHEDMVETAVFGTENNLIITGSLDALIQVWSLSEQGTLLDILEGVGAPVSLLARGGALVASASPQSSSFKVWDLSDAHRSRVPAPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDLAEGEEQDSLDTSSEIRCLEVAEQRKLLFTGLVSGVVLVFPLNSRQDVICIPPPEARKAINCMSLSKCEDRLAIAYDNIVLVLDITSGDPCPVIDGPRYTFYTQLPETLSSVAILTDYRVVYSMTNGDLFLYECATSKAFPLETHRSRVACVEVSHKEQLVVSGSEDALLCLWDLQARKWKFEMSYTAPC

Target ORF information:

RefSeq Version	XM_024451466.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451466.1

ORF Insert Sequence:

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGTTTCCT CCAAAGGGGA CAGATTGCTG 
GAGAAGCTTC CAGATGCTGT GAGGTTCCTG GTGGTCTCTG AAGATGAGTC CCTCCTCGCC 
GCAGGCTTTG GAAGATCGGT GCGGATATTC TTGGCGGACT CGAGGGGCTT TCGCCGATTC 
ATGGCCATGG ATCTGGAACA TGAAGACATG GTGGAGACGG CTGTTTTTGG TACTGAGAAC 
AACCTGATCA TCACGGGGTC CCTTGATGCG CTCATTCAGG TGTGGAGTCT GTCAGAACAG 
GGGACCCTTC TGGACATCCT GGAAGGCGTC GGGGCCCCCG TGAGCCTGCT GGCCCGCGGC 
GGGGCTTTGG TGGCATCTGC TTCCCCACAG TCCTCATCTT TCAAGGTCTG GGATCTCAGC 
GATGCTCATA GGTCCCGGGT GCCTGCACCA TTTCTGGACC GCACCGGCCT CACCGCAGTG 
TCCCACAATG GAAGCTACGT CTACTTCCCC AAAATTGGGG ACAAAAACAA AGTCACTATT 
TGGGACTTGG CAGAAGGCGA GGAACAAGAT TCCCTGGACA CCTCCAGTGA GATCAGGTGT 
CTGGAGGTTG CTGAGCAGCG CAAGCTCCTA TTTACGGGCC TCGTGTCGGG GGTCGTCCTT 
GTGTTCCCCC TGAATTCCAG GCAGGACGTG ATATGCATTC CCCCTCCCGA GGCCCGGAAA 
GCAATCAACT GCATGTCCCT GAGCAAGTGC GAGGACCGCC TGGCCATCGC CTATGACAAC 
ATCGTCCTGG TGCTGGACAT CACCTCCGGG GACCCCTGCC CGGTCATCGA TGGGCCAAGA 
TACACCTTTT ACACTCAGCT GCCCGAGACC CTCTCCAGCG TGGCCATTCT GACGGACTAC 
CGCGTGGTCT ACAGCATGAC CAATGGGGAC CTCTTTCTTT ACGAGTGTGC AACTTCCAAA 
GCGTTTCCCT TGGAGACCCA CAGGAGCCGA GTTGCCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGTGG TCAGCGGGTC TGAGGATGCC CTGCTGTGTC TCTGGGACCT GCAGGCACGC 
AAGTGGAAAT TCGAGATGAG CTACACGGCA CCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_024451467.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307235.1 Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X6, mRNA.

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CloneID	OHu104660
Clone ID Related Accession (Same CDS sequence)	XM_024451467.1
Accession Version	XM_024451467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGTTTCCT CCAAAGGGGA CAGATTGCTG 
GAGAAGCTTC CAGATGCTGT GAGGTTCCTG GTGGTCTCTG AAGATGAGTC CCTCCTCGCC 
GCAGGCTTTG GAAGATCGGT GCGGATATTC TTGGCGGACT CGAGGGGCTT TCGCCGATTC 
ATGGCCATGG ATCTGGAACA TGAAGACATG GTGGAGACGG CTGTTTTTGG TACTGAGAAC 
AACCTGATCA TCACGGGGTC CCTTGATGCG CTCATTCAGG TGTGGAGTCT GTCAGAACAG 
GGGACCCTTC TGGACATCCT GGAAGGCGTC GGGGCCCCCG TGAGCCTGCT GGCCCGCGGC 
GGGGCTTTGG TGGCATCTGC TTCCCCACAG TCCTCATCTT TCAAGGTCTG GGATCTCAGC 
GATGCTCATA GGTCCCGGGT GCCTGCACCA TTTCTGGACC GCACCGGCCT CACCGCAGTG 
TCCCACAATG GAAGCTACGT CTACTTCCCC AAAATTGGGG ACAAAAACAA AGTCACTATT 
TGGGACTTGG CAGAAGGTTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307235.1
CDS	607..4350
Translation	MQRGKPCRALPTLKCQTFCQRHGLMFEVVDLRWGIRNIEATDHLTTELCLEEVDRCWKTSIGPAFVALIGDQYGPCLIPSRIDEKEWEVLRDHLTARPSDLELVARYFQRDENAFPPTYVLQAPGTGEACEPEEATLTSVLRSGAQEARRLGLITQEQWQHYHRSVIEWEIERSLLSSEDREQGATVFLREIQDLHKHILEDCALRMVDRLADGCLDADAQNLLSSLKSHITDMHPGVLKTHRLPWSRDLVNPKNKTHACYLKELGEQFVVRANHQVLTRLRELDTAGQELAWLYQEIRHHLWQSSEVIQTFCGRQELLARLGQQLRHDDSKQHTPLVLFGPPGIGKTALMCKLAEQMPRLLGHKTVTVLRLLGTSQMSSDARGLLKSICFQVCLAYGLPLPPAQVLDAHTRVVQFFHTLLHTVSCRNFESLVLLLDAMDDLDSVRHARRVPWLPLNCPPRVHLILSACSGALGVLDTLQRVLLDPEAYWEVKPLSGNQGQQMIQLLLAAARRTLSPVHTDLLWASLPECGNPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCTRLEQTHGQLLVAHVLGYIVSSRHGLSEAELKDVLSLDDEVLQDVYRDWTPPSKELLRFPPLLWVRLRRDLGYYLARRPVDGFTLLAIAHRQLVEVVRERYLSGSERAKRHGVLADFFSGTWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSHTVANLRKLKELPYHLLHSGRLEELKQEVLGSMSWISCRGISGGIEDLLDDFDLCAPHLDSPEVGLVREALQLCRPAVELRGMERSLLYTELLARLHFFATSHPALVGQLCQQAQSWFQLCAHPVLVPLGGFLQPPGGPLRATLSGCHKGITAMAWGVEEKLLVIGTQDGIMAVWDMEEQHVIHMLTGHTGEVRCVKIFAKGTLANSASKDYTLHLWNLLSGQEKFTIWDGGSKNPAEPQIWNLHVDEAHKVVYSASGSKINAWNLETAEPVFHILGDASDPWMCMAVLASQATLLTVSRDGVVSLWSSATGKLQGKQHMSSIKEETPTCAVSVQKQGKLVTGFSNGSISLVSSKGDRLLEKLPDAVRFLVVSEDESLLAAGFGRSVRIFLADSRGFRRFMAMDLEHEDMVETAVFGTENNLIITGSLDALIQVWSLSEQGTLLDILEGVGAPVSLLARGGALVASASPQSSSFKVWDLSDAHRSRVPAPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDLAEGW

Target ORF information:

RefSeq Version	XM_024451467.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451467.1

ORF Insert Sequence:

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGTTTCCT CCAAAGGGGA CAGATTGCTG 
GAGAAGCTTC CAGATGCTGT GAGGTTCCTG GTGGTCTCTG AAGATGAGTC CCTCCTCGCC 
GCAGGCTTTG GAAGATCGGT GCGGATATTC TTGGCGGACT CGAGGGGCTT TCGCCGATTC 
ATGGCCATGG ATCTGGAACA TGAAGACATG GTGGAGACGG CTGTTTTTGG TACTGAGAAC 
AACCTGATCA TCACGGGGTC CCTTGATGCG CTCATTCAGG TGTGGAGTCT GTCAGAACAG 
GGGACCCTTC TGGACATCCT GGAAGGCGTC GGGGCCCCCG TGAGCCTGCT GGCCCGCGGC 
GGGGCTTTGG TGGCATCTGC TTCCCCACAG TCCTCATCTT TCAAGGTCTG GGATCTCAGC 
GATGCTCATA GGTCCCGGGT GCCTGCACCA TTTCTGGACC GCACCGGCCT CACCGCAGTG 
TCCCACAATG GAAGCTACGT CTACTTCCCC AAAATTGGGG ACAAAAACAA AGTCACTATT 
TGGGACTTGG CAGAAGGTTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_011527934.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526236.1 Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu78812
Clone ID Related Accession (Same CDS sequence)	XM_011527934.1
Accession Version	XM_011527934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACC TGGACTCTGT CCGCCATGCT CGGAGGGTTC CCTGGCTGCC TCTCAACTGC 
CCCCCGAGGG TGCACCTCAT CCTCTCAGCT TGCTCGGGGG CACTGGGGGT TTTGGACACC 
TTGCAGCGGG TGCTCCTGGA CCCGGAGGCC TACTGGGAGG TGAAGCCCCT TTCCGGAAAC 
CAAGGCCAGC AGATGATCCA ACTCCTGCTG GCAGCTGCAA GGAGGACGCT GAGCCCGGTG 
CACACAGATT TGCTCTGGGC CAGCCTCCCA GAGTGTGGGA ACCCAGGGCG GCTGAGGCTG 
GCGTTTGAGG AAGCCCGGAA ATGGGCCTCT TTCACCGTGC CTGTCCCGCT GGCCACCACC 
GCAGAGGAAG CCACGCACCA ACTCTGCACC CGCCTGGAGC AGACACACGG GCAGCTCCTC 
GTGGCCCACG TGCTGGGCTA CATTGTGTCT TCCCGACACG GTCTCTCGGA GGCGGAGCTG 
AAGGATGTTT TGTCCCTGGA CGACGAGGTC CTGCAGGATG TGTACCGAGA TTGGACCCCG 
CCCAGCAAGG AGCTGCTGCG CTTCCCGCCC CTGCTGTGGG TGCGGCTTCG TCGGGATCTG 
GGATACTACT TGGCCCGGCG GCCCGTGGAT GGCTTCACCC TCCTGGCCAT TGCCCACAGA 
CAGCTGGTCG AGGTGGTCCG TGAGCGCTAC CTGTCAGGAT CCGAGAGAGC CAAGAGGCAT 
GGCGTCCTGG CCGACTTCTT CTCAGGGACC TGGAGCCAGG GTACCAAGAA GCTCATCACT 
CTGCCACTTG TGGGGAAACC ACTGAACTTG GACCGAAAGG TGGCCCCGCA GCCTCTGTGG 
TTCTCACATA CGGTTGCAAA CCTGCGGAAG CTGAAGGAGT TGCCCTATCA CCTGCTTCAC 
TCGGGCCGCC TGGAGGAGCT GAAACAGGAG GTTCTGGGCA GCATGAGCTG GATTTCCTGC 
CGGGGCATCT CTGGGGGCAT TGAAGACCTG CTGGATGACT TTGACCTGTG TGCCCCTCAC 
CTGGACTCCC CTGAGGTTGG CCTGGTCCGT GAAGCCCTCC AGCTCTGCCG CCCTGCTGTG 
GAGCTCCGAG GCATGGAGAG GAGCCTCCTG TACACAGAAC TGCTGGCCAG ACTCCATTTC 
TTCGCCACCT CACATCCAGC ACTGGTGGGA CAGCTATGCC AACAGGCCCA GAGCTGGTTC 
CAGTTGTGCG CACACCCTGT GCTGGTGCCC CTCGGAGGAT TCCTCCAGCC CCCGGGAGGA 
CCCCTCCGGG CAACTCTCAG CGGCTGTCAC AAAGGCATCA CCGCCATGGC ATGGGGTGTG 
GAGGAGAAGC TGCTGGTGAT TGGCACCCAG GATGGCATCA TGGCTGTGTG GGACATGGAA 
GAGCAGCATG TGATCCACAT GCTAACTGGA CACACAGGAG AGGTGAGGTG TGTGAAAATA 
TTTGCCAAAG GGACCCTCGC CAACTCTGCT TCAAAGGATT ACACGCTGCA CTTGTGGAAC 
TTACTCTCTG GCCAGGAGAA ATTTACCATT TGGGATGGAG GCTCAAAAAA TCCCGCTGAA 
CCTCAGATCT GGAACCTTCA TGTGGATGAG GCACACAAAG TTGTGTATTC AGCATCTGGC 
TCAAAGATCA ATGCTTGGAA TCTGGAAACT GCAGAGCCGG TATTCCATAT CCTGGGAGAT 
GCCTCTGATC CTTGGATGTG CATGGCCGTG CTGGCCTCCC AGGCCACACT GCTGACAGTG 
TCCAGGGATG GTGTGGTCAG TCTGTGGAGC TCAGCTACGG GAAAACTTCA GGGGAAGCAA 
CATATGTCCA GCATCAAAGA AGAAACACCT ACCTGTGCCG TCTCAGTCCA GAAGCAAGGA 
AAGCTTGTTA CCGGGTTTAG CAATGGCTCC ATCTCTTTGG TTTCCTCCAA AGGGGACAGA 
TTGCTGGAGA AGCTTCCAGA TGCTGTGAGG TTCCTGGTGG TCTCTGAAGA TGAGTCCCTC 
CTCGCCGCAG GCTTTGGAAG ATCGGTGCGG ATATTCTTGG CGGACTCGAG GGGCTTTCGC 
CGATTCATGG CCATGGATCT GGAACATGAA GACATGGTGG AGACGGCTGT TTTTGGTACT 
GAGAACAACC TGATCATCAC GGGGTCCCTT GATGCGCTCA TTCAGGTGTG GAGTCTGTCA 
GAACAGGGGA CCCTTCTGGA CATCCTGGAA GGCGTCGGGG CCCCCGTGAG CCTGCTGGCC 
CGCGGCGGGG CTTTGGTGGC ATCTGCTTCC CCACAGTCCT CATCTTTCAA GGTCTGGGAT 
CTCAGCGATG CTCATAGGTC CCGGGTGCCT GCACCATTTC TGGACCGCAC CGGCCTCACC 
GCAGTGTCCC ACAATGGAAG CTACGTCTAC TTCCCCAAAA TTGGGGACAA AAACAAAGTC 
ACTATTTGGG ACTTGGCAGA AGGCGAGGAA CAAGATTCCC TGGACACCTC CAGTGAGATC 
AGGTGTCTGG AGGTTGCTGA GCAGCGCAAG CTCCTATTTA CGGGCCTCGT GTCGGGGGTC 
GTCCTTGTGT TCCCCCTGAA TTCCAGGCAG GACGTGATAT GCATTCCCCC TCCCGAGGCC 
CGGAAAGCAA TCAACTGCAT GTCCCTGAGC AAGTGCGAGG ACCGCCTGGC CATCGCCTAT 
GACAACATCG TCCTGGTGCT GGACATCACC TCCGGGGACC CCTGCCCGGT CATCGATGGG 
CCAAGATACA CCTTTTACAC TCAGCTGCCC GAGACCCTCT CCAGCGTGGC CATTCTGACG 
GACTACCGCG TGGTCTACAG CATGACCAAT GGGGACCTCT TTCTTTACGA GTGTGCAACT 
TCCAAAGCGT TTCCCTTGGA GACCCACAGG AGCCGAGTTG CCTGTGTGGA GGTCAGCCAC 
AAGGAGCAGC TGGTGGTCAG CGGGTCTGAG GATGCCCTGC TGTGTCTCTG GGACCTGCAG 
GCACGCAAGT GGAAATTCGA GATGAGCTAC ACGAGTTCTT ACTGCAGAGG GGTCCAATGT 
GCTTGCTTCT CCAAGGATGA CAAGTATGTG TACGTGGGCT TAAAGGATCG CTCTATACTT 
GTTTGGAGTG TGCTGGATGG CACCCTGCTG ACAGTCCAGT TTGTCCATGC TGTGGTAAAC 
AGAATCATCC CAACCACCAG TGGCTTCATT GCCCCCACCA GGCATGGTTA TCTGATCCGA 
GAGAATTTCC AGTGCCTTTC AGCAAAAGCC TCACCGCAGG ACCCTCTTAA GAACTTCAAG 
AAGGCAATGT GGATGGTCAA ATCCAGGCAG AGAGAGGAGC TAGTTGCAGC TGCAGGAGCT 
CCCCAGGACT TGGAGTCAGA AAGTGCCCAG GGAAATGAAA CCAAATCAAA CAAATGCTCA 
CAAGTGTGCC TGATAGTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526236.1
CDS	126..3506
Translation	MDDLDSVRHARRVPWLPLNCPPRVHLILSACSGALGVLDTLQRVLLDPEAYWEVKPLSGNQGQQMIQLLLAAARRTLSPVHTDLLWASLPECGNPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCTRLEQTHGQLLVAHVLGYIVSSRHGLSEAELKDVLSLDDEVLQDVYRDWTPPSKELLRFPPLLWVRLRRDLGYYLARRPVDGFTLLAIAHRQLVEVVRERYLSGSERAKRHGVLADFFSGTWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSHTVANLRKLKELPYHLLHSGRLEELKQEVLGSMSWISCRGISGGIEDLLDDFDLCAPHLDSPEVGLVREALQLCRPAVELRGMERSLLYTELLARLHFFATSHPALVGQLCQQAQSWFQLCAHPVLVPLGGFLQPPGGPLRATLSGCHKGITAMAWGVEEKLLVIGTQDGIMAVWDMEEQHVIHMLTGHTGEVRCVKIFAKGTLANSASKDYTLHLWNLLSGQEKFTIWDGGSKNPAEPQIWNLHVDEAHKVVYSASGSKINAWNLETAEPVFHILGDASDPWMCMAVLASQATLLTVSRDGVVSLWSSATGKLQGKQHMSSIKEETPTCAVSVQKQGKLVTGFSNGSISLVSSKGDRLLEKLPDAVRFLVVSEDESLLAAGFGRSVRIFLADSRGFRRFMAMDLEHEDMVETAVFGTENNLIITGSLDALIQVWSLSEQGTLLDILEGVGAPVSLLARGGALVASASPQSSSFKVWDLSDAHRSRVPAPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDLAEGEEQDSLDTSSEIRCLEVAEQRKLLFTGLVSGVVLVFPLNSRQDVICIPPPEARKAINCMSLSKCEDRLAIAYDNIVLVLDITSGDPCPVIDGPRYTFYTQLPETLSSVAILTDYRVVYSMTNGDLFLYECATSKAFPLETHRSRVACVEVSHKEQLVVSGSEDALLCLWDLQARKWKFEMSYTSSYCRGVQCACFSKDDKYVYVGLKDRSILVWSVLDGTLLTVQFVHAVVNRIIPTTSGFIAPTRHGYLIRENFQCLSAKASPQDPLKNFKKAMWMVKSRQREELVAAAGAPQDLESESAQGNETKSNKCSQVCLIV

Target ORF information:

RefSeq Version	XM_011527934.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527934.1

ORF Insert Sequence:

ATGGATGACC TGGACTCTGT CCGCCATGCT CGGAGGGTTC CCTGGCTGCC TCTCAACTGC 
CCCCCGAGGG TGCACCTCAT CCTCTCAGCT TGCTCGGGGG CACTGGGGGT TTTGGACACC 
TTGCAGCGGG TGCTCCTGGA CCCGGAGGCC TACTGGGAGG TGAAGCCCCT TTCCGGAAAC 
CAAGGCCAGC AGATGATCCA ACTCCTGCTG GCAGCTGCAA GGAGGACGCT GAGCCCGGTG 
CACACAGATT TGCTCTGGGC CAGCCTCCCA GAGTGTGGGA ACCCAGGGCG GCTGAGGCTG 
GCGTTTGAGG AAGCCCGGAA ATGGGCCTCT TTCACCGTGC CTGTCCCGCT GGCCACCACC 
GCAGAGGAAG CCACGCACCA ACTCTGCACC CGCCTGGAGC AGACACACGG GCAGCTCCTC 
GTGGCCCACG TGCTGGGCTA CATTGTGTCT TCCCGACACG GTCTCTCGGA GGCGGAGCTG 
AAGGATGTTT TGTCCCTGGA CGACGAGGTC CTGCAGGATG TGTACCGAGA TTGGACCCCG 
CCCAGCAAGG AGCTGCTGCG CTTCCCGCCC CTGCTGTGGG TGCGGCTTCG TCGGGATCTG 
GGATACTACT TGGCCCGGCG GCCCGTGGAT GGCTTCACCC TCCTGGCCAT TGCCCACAGA 
CAGCTGGTCG AGGTGGTCCG TGAGCGCTAC CTGTCAGGAT CCGAGAGAGC CAAGAGGCAT 
GGCGTCCTGG CCGACTTCTT CTCAGGGACC TGGAGCCAGG GTACCAAGAA GCTCATCACT 
CTGCCACTTG TGGGGAAACC ACTGAACTTG GACCGAAAGG TGGCCCCGCA GCCTCTGTGG 
TTCTCACATA CGGTTGCAAA CCTGCGGAAG CTGAAGGAGT TGCCCTATCA CCTGCTTCAC 
TCGGGCCGCC TGGAGGAGCT GAAACAGGAG GTTCTGGGCA GCATGAGCTG GATTTCCTGC 
CGGGGCATCT CTGGGGGCAT TGAAGACCTG CTGGATGACT TTGACCTGTG TGCCCCTCAC 
CTGGACTCCC CTGAGGTTGG CCTGGTCCGT GAAGCCCTCC AGCTCTGCCG CCCTGCTGTG 
GAGCTCCGAG GCATGGAGAG GAGCCTCCTG TACACAGAAC TGCTGGCCAG ACTCCATTTC 
TTCGCCACCT CACATCCAGC ACTGGTGGGA CAGCTATGCC AACAGGCCCA GAGCTGGTTC 
CAGTTGTGCG CACACCCTGT GCTGGTGCCC CTCGGAGGAT TCCTCCAGCC CCCGGGAGGA 
CCCCTCCGGG CAACTCTCAG CGGCTGTCAC AAAGGCATCA CCGCCATGGC ATGGGGTGTG 
GAGGAGAAGC TGCTGGTGAT TGGCACCCAG GATGGCATCA TGGCTGTGTG GGACATGGAA 
GAGCAGCATG TGATCCACAT GCTAACTGGA CACACAGGAG AGGTGAGGTG TGTGAAAATA 
TTTGCCAAAG GGACCCTCGC CAACTCTGCT TCAAAGGATT ACACGCTGCA CTTGTGGAAC 
TTACTCTCTG GCCAGGAGAA ATTTACCATT TGGGATGGAG GCTCAAAAAA TCCCGCTGAA 
CCTCAGATCT GGAACCTTCA TGTGGATGAG GCACACAAAG TTGTGTATTC AGCATCTGGC 
TCAAAGATCA ATGCTTGGAA TCTGGAAACT GCAGAGCCGG TATTCCATAT CCTGGGAGAT 
GCCTCTGATC CTTGGATGTG CATGGCCGTG CTGGCCTCCC AGGCCACACT GCTGACAGTG 
TCCAGGGATG GTGTGGTCAG TCTGTGGAGC TCAGCTACGG GAAAACTTCA GGGGAAGCAA 
CATATGTCCA GCATCAAAGA AGAAACACCT ACCTGTGCCG TCTCAGTCCA GAAGCAAGGA 
AAGCTTGTTA CCGGGTTTAG CAATGGCTCC ATCTCTTTGG TTTCCTCCAA AGGGGACAGA 
TTGCTGGAGA AGCTTCCAGA TGCTGTGAGG TTCCTGGTGG TCTCTGAAGA TGAGTCCCTC 
CTCGCCGCAG GCTTTGGAAG ATCGGTGCGG ATATTCTTGG CGGACTCGAG GGGCTTTCGC 
CGATTCATGG CCATGGATCT GGAACATGAA GACATGGTGG AGACGGCTGT TTTTGGTACT 
GAGAACAACC TGATCATCAC GGGGTCCCTT GATGCGCTCA TTCAGGTGTG GAGTCTGTCA 
GAACAGGGGA CCCTTCTGGA CATCCTGGAA GGCGTCGGGG CCCCCGTGAG CCTGCTGGCC 
CGCGGCGGGG CTTTGGTGGC ATCTGCTTCC CCACAGTCCT CATCTTTCAA GGTCTGGGAT 
CTCAGCGATG CTCATAGGTC CCGGGTGCCT GCACCATTTC TGGACCGCAC CGGCCTCACC 
GCAGTGTCCC ACAATGGAAG CTACGTCTAC TTCCCCAAAA TTGGGGACAA AAACAAAGTC 
ACTATTTGGG ACTTGGCAGA AGGCGAGGAA CAAGATTCCC TGGACACCTC CAGTGAGATC 
AGGTGTCTGG AGGTTGCTGA GCAGCGCAAG CTCCTATTTA CGGGCCTCGT GTCGGGGGTC 
GTCCTTGTGT TCCCCCTGAA TTCCAGGCAG GACGTGATAT GCATTCCCCC TCCCGAGGCC 
CGGAAAGCAA TCAACTGCAT GTCCCTGAGC AAGTGCGAGG ACCGCCTGGC CATCGCCTAT 
GACAACATCG TCCTGGTGCT GGACATCACC TCCGGGGACC CCTGCCCGGT CATCGATGGG 
CCAAGATACA CCTTTTACAC TCAGCTGCCC GAGACCCTCT CCAGCGTGGC CATTCTGACG 
GACTACCGCG TGGTCTACAG CATGACCAAT GGGGACCTCT TTCTTTACGA GTGTGCAACT 
TCCAAAGCGT TTCCCTTGGA GACCCACAGG AGCCGAGTTG CCTGTGTGGA GGTCAGCCAC 
AAGGAGCAGC TGGTGGTCAG CGGGTCTGAG GATGCCCTGC TGTGTCTCTG GGACCTGCAG 
GCACGCAAGT GGAAATTCGA GATGAGCTAC ACGAGTTCTT ACTGCAGAGG GGTCCAATGT 
GCTTGCTTCT CCAAGGATGA CAAGTATGTG TACGTGGGCT TAAAGGATCG CTCTATACTT 
GTTTGGAGTG TGCTGGATGG CACCCTGCTG ACAGTCCAGT TTGTCCATGC TGTGGTAAAC 
AGAATCATCC CAACCACCAG TGGCTTCATT GCCCCCACCA GGCATGGTTA TCTGATCCGA 
GAGAATTTCC AGTGCCTTTC AGCAAAAGCC TCACCGCAGG ACCCTCTTAA GAACTTCAAG 
AAGGCAATGT GGATGGTCAA ATCCAGGCAG AGAGAGGAGC TAGTTGCAGC TGCAGGAGCT 
CCCCAGGACT TGGAGTCAGA AAGTGCCCAG GGAAATGAAA CCAAATCAAA CAAATGCTCA 
CAAGTGTGCC TGATAGTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_011527935.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526237.1 Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu78813
Clone ID Related Accession (Same CDS sequence)	XM_011527935.1
Accession Version	XM_011527935.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGCTTTGG AAGATCGGTG CGGATATTCT 
TGGCGGACTC GAGGGGCTTT CGCCGATTCA TGGCCATGGA TCTGGAACAT GAAGACATGG 
TGGAGACGGC TGTTTTTGGT ACTGAGAACA ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526237.1
CDS	607..3942
Translation	MQRGKPCRALPTLKCQTFCQRHGLMFEVVDLRWGIRNIEATDHLTTELCLEEVDRCWKTSIGPAFVALIGDQYGPCLIPSRIDEKEWEVLRDHLTARPSDLELVARYFQRDENAFPPTYVLQAPGTGEACEPEEATLTSVLRSGAQEARRLGLITQEQWQHYHRSVIEWEIERSLLSSEDREQGATVFLREIQDLHKHILEDCALRMVDRLADGCLDADAQNLLSSLKSHITDMHPGVLKTHRLPWSRDLVNPKNKTHACYLKELGEQFVVRANHQVLTRLRELDTAGQELAWLYQEIRHHLWQSSEVIQTFCGRQELLARLGQQLRHDDSKQHTPLVLFGPPGIGKTALMCKLAEQMPRLLGHKTVTVLRLLGTSQMSSDARGLLKSICFQVCLAYGLPLPPAQVLDAHTRVVQFFHTLLHTVSCRNFESLVLLLDAMDDLDSVRHARRVPWLPLNCPPRVHLILSACSGALGVLDTLQRVLLDPEAYWEVKPLSGNQGQQMIQLLLAAARRTLSPVHTDLLWASLPECGNPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCTRLEQTHGQLLVAHVLGYIVSSRHGLSEAELKDVLSLDDEVLQDVYRDWTPPSKELLRFPPLLWVRLRRDLGYYLARRPVDGFTLLAIAHRQLVEVVRERYLSGSERAKRHGVLADFFSGTWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSHTVANLRKLKELPYHLLHSGRLEELKQEVLGSMSWISCRGISGGIEDLLDDFDLCAPHLDSPEVGLVREALQLCRPAVELRGMERSLLYTELLARLHFFATSHPALVGQLCQQAQSWFQLCAHPVLVPLGGFLQPPGGPLRATLSGCHKGITAMAWGVEEKLLVIGTQDGIMAVWDMEEQHVIHMLTGHTGEVRCVKIFAKGTLANSASKDYTLHLWNLLSGQEKFTIWDGGSKNPAEPQIWNLHVDEAHKVVYSASGSKINAWNLETAEPVFHILGDASDPWMCMAVLASQATLLTVSRDGVVSLWSSATGKLQGKQHMSSIKEETPTCAVSVQKQGKLVTGFSNGSISLALEDRCGYSWRTRGAFADSWPWIWNMKTWWRRLFLVLRTT

Target ORF information:

RefSeq Version	XM_011527935.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527935.1

ORF Insert Sequence:

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGCTTTGG AAGATCGGTG CGGATATTCT 
TGGCGGACTC GAGGGGCTTT CGCCGATTCA TGGCCATGGA TCTGGAACAT GAAGACATGG 
TGGAGACGGC TGTTTTTGGT ACTGAGAACA ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( NM_001290355.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001277284.1 Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09297
Clone ID Related Accession (Same CDS sequence)	NM_001290355.2 , NM_001290355.3
Accession Version	NM_001290355.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	NACHT domain- and WD repeat-containing protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX648940.1, AC008737.11, BC117698.1, AK090685.1 and CB242932.1. On Dec 29, 2016 this sequence version replaced NM_001290355.1. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, initiates translation at an alternate start codon and contains an alternate exon in the 3' coding region, resulting in a translational frameshift, compared to variant 1. It encodes isoform 2, which is shorter and has distinct N- and C-termini, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BX648940.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153427, SAMEA2158569 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTCAC AATTCCACAT GGCTGGGGAG GCCTCACAAT CATGGCTGAA GGCAAAGGAG 
AAGCAAAGAG GCACATCTTA CATGGTGGCA GTCATTGAGT GGGAGATAGA GCGGAGCCTG 
CTGAGCTCAG AGGACCGGGA ACAGGGAGCC ACCGTCTTCC TTAGAGAGAT CCAAGACCTC 
CACAAACACA TCCTTGAAGA CTGCGCCCTT AGGATGGTGG ACCGGCTCGC GGATGGCTGC 
CTGGACGCTG ATGCCCAGAA CCTTCTCAGC AGCCTCAAAA GTCACATCAC TGACATGCAC 
CCAGGGGTCC TCAAGACCCA CCGCCTGCCG TGGAGCCGCG ACTTGGTGAA CCCCAAGAAC 
AAGACTCACG CCTGCTACCT GAAGGAGCTG GGTGAGCAGT TTGTGGTGAG GGCCAATCAC 
CAGGTCCTCA CACGCCTCCG TGAGCTGGAT ACGGCCGGAC AGGAGTTGGC GTGGCTCTAC 
CAAGAGATCC GCCACCACCT TTGGCAGAGC TCGGAGGTCA TTCAGACCTT CTGCGGACGC 
CAGGAACTCC TGGCCCGGCT TGGGCAGCAG CTCAGGCACG ATGACAGCAA GCAGCACACC 
CCCCTGGTAC TCTTTGGGCC CCCAGGCATT GGAAAGACAG CCCTGATGTG CAAGCTGGCT 
GAGCAGATGC CAAGGCTGCT GGGGCACAAG ACAGTGACCG TCCTGCGGCT GCTGGGGACG 
TCACAAATGA GCTCAGATGC CCGTGGCCTG CTGAAGAGCA TCTGCTTCCA GGTGTGCCTG 
GCCTATGGGC TGCCCTTGCC CCCTGCCCAG GTTCTGGACG CCCACACCAG GGTGGTCCAG 
TTTTTCCATA CCCTCCTCCA CACTGTCTCT TGCAGAAACT TCGAGTCTCT CGTGCTCCTG 
CTGGATGCTA TGGATGACCT GGACTCTGTC CGCCATGCTC GGAGGGTTCC CTGGCTGCCT 
CTCAACTGCC CCCCGAGGGT GCACCTCATC CTCTCAGCTT GCTCGGGGGC ACTGGGGGTT 
TTGGACACCT TGCAGCGGGT GCTCCTGGAC CCGGAGGCCT ACTGGGAGGT GAAGCCCCTT 
TCCGGAAACC AAGGCCAGCA GATGATCCAA CTCCTGCTGG CAGCTGCAAG GAGGACGCTG 
AGCCCGGTGC ACACAGATTT GCTCTGGGCC AGCCTCCCAG AGTGTGGGAA CCCAGGGCGG 
CTGAGGCTGG CGTTTGAGGA AGCCCGGAAA TGGGCCTCTT TCACCGTGCC TGTCCCGCTG 
GCCACCACCG CAGAGGAAGC CACGCACCAA CTCTGCACCC GCCTGGAGCA GACACACGGG 
CAGCTCCTCG TGGCCCACGT GCTGGGCTAC ATTGTGTCTT CCCGACACGG TCTCTCGGAG 
GCGGAGCTGA AGGATGTTTT GTCCCTGGAC GACGAGGTCC TGCAGGATGT GTACCGAGAT 
TGGACCCCGC CCAGCAAGGA GCTGCTGCGC TTCCCGCCCC TGCTGTGGGT GCGGCTTCGT 
CGGGATCTGG GATACTACTT GGCCCGGCGG CCCGTGGATG GCTTCACCCT CCTGGCCATT 
GCCCACAGAC AGCTGGTCGA GGTGGTCCGT GAGCGCTACC TGTCAGGATC CGAGAGAGCC 
AAGAGGCATG GCGTCCTGGC CGACTTCTTC TCAGGGACCT GGAGCCAGGG TACCAAGAAG 
CTCATCACTC TGCCACTTGT GGGGAAACCA CTGAACTTGG ACCGAAAGGT GGCCCCGCAG 
CCTCTGTGGT TCTCACATAC GGTTGCAAAC CTGCGGAAGC TGAAGGAGTT GCCCTATCAC 
CTGCTTCACT CGGGCCGCCT GGAGGAGCTG AAACAGGAGG TTCTGGGCAG CATGAGCTGG 
ATTTCCTGCC GGGGCATCTC TGGGGGCATT GAAGACCTGC TGGATGACTT TGACCTGTGT 
GCCCCTCACC TGGACTCCCC TGAGGTTGGC CTGGTCCGTG AAGCCCTCCA GCTCTGCCGC 
CCTGCTGTGG AGCTCCGAGG CATGGAGAGG AGCCTCCTGT ACACAGAACT GCTGGCCAGA 
CTCCATTTCT TCGCCACCTC ACATCCAGCA CTGGTGGGAC AGCTATGCCA ACAGGCCCAG 
AGCTGGTTCC AGTTGTGCGC ACACCCTGTG CTGGTGCCCC TCGGAGGATT CCTCCAGCCC 
CCGGGAGGAC CCCTCCGGGC AACTCTCAGC GGCTGTCACA AAGGCATCAC CGCCATGGCA 
TGGGGTGTGG AGGAGAAGCT GCTGGTGATT GGCACCCAGG ATGGCATCAT GGCTGTGTGG 
GACATGGAAG AGCAGCATGT GATCCACATG CTAACTGGAC ACACAGGAGA GGTGAGGTGT 
GTGAAAATAT TTGCCAAAGG GACCCTCGCC AACTCTGCTT CAAAGGATTA CACGCTGCAC 
TTGTGGAACT TACTCTCTGG CCAGGAGAAA TTTACCATTT GGGATGGAGG CTCAAAAAAT 
CCCGCTGAAC CTCAGATCTG GAACCTTCAT GTGGATGAGG CACACAAAGT TGTGTATTCA 
GCATCTGGCT CAAAGATCAA TGCTTGGAAT CTGGAAACTG CAGAGCCGGT ATTCCATATC 
CTGGGAGATG CCTCTGATCC TTGGATGTGC ATGGCCGTGC TGGCCTCCCA GGCCACACTG 
CTGACAGTGT CCAGGGATGG TGTGGTCAGT CTGTGGAGCT CAGCTACGGG AAAACTTCAG 
GGGAAGCAAC ATATGTCCAG CATCAAAGAA GAAACACCTA CCTGTGCCGT CTCAGTCCAG 
AAGCAAGGAA AGCTTGTTAC CGGGTTTAGC AATGGCTCCA TCTCTTTGGT TTCCTCCAAA 
GGGGACAGAT TGCTGGAGAA GCTTCCAGAT GCTGTGAGGT TCCTGGTGGT CTCTGAAGAT 
GAGTCCCTCC TCGCCGCAGG CTTTGGAAGA TCGGTGCGGA TATTCTTGGC GGACTCGAGG 
GGCTTTCGCC GATTCATGGC CATGGATCTG GAACATGAAG ACATGGTGGA GACGGCTGTT 
TTTGGTACTG AGAACAACCT GATCATCACG GGGTCCCTTG ATGCGCTCAT TCAGGTGTGG 
AGTCTGTCAG AACAGGGGAC CCTTCTGGAC ATCCTGGAAG GCGTCGGGGC CCCCGTGAGC 
CTGCTGGCCC GCGGCGGGGC TTTGGTGGCA TCTGCTTCCC CACAGTCCTC ATCTTTCAAG 
GTCTGGGATC TCAGCGATGC TCATAGGTCC CGGGTGCCTG CACCATTTCT GGACCGCACC 
GGCCTCACCG CAGTGTCCCA CAATGGAAGC TACGTCTACT TCCCCAAAAT TGGGGACAAA 
AACAAAGTCA CTATTTGGGA CTTGGCAGAA GGCGAGGAAC AAGATTCCCT GGACACCTCC 
AGTGAGATCA GGTGTCTGGA GGTTGCTGAG CAGCGCAAGC TCCTATTTAC GGGCCTCGTG 
TCGGGGGTCG TCCTTGTGTT CCCCCTGAAT TCCAGGCAGG ACGTGATATG CATTCCCCCT 
CCCGAGGCCC GGAAAGCAAT CAACTGCATG TCCCTGAGCA AGTGCGAGGA CCGCCTGGCC 
ATCGCCTATG ACAACATCGT CCTGGTGCTG GACATCACCT CCGGGGACCC CTGCCCGGTC 
ATCGATGGGC CAAGATACAC CTTTTACACT CAGCTGCCCG AGACCCTCTC CAGCGTGGCC 
ATTCTGACGG ACTACCGCGT GGTCTACAGC ATGACCAATG GGGACCTCTT TCTTTACGAG 
TGTGCAACTT CCAAAGCGTT TCCCTTGGAG ACCCACAGGA GCCGAGTTGC CTGTGTGGAG 
GTCAGCCACA AGGAGCAGCT GGTGGTCAGC GGGTCTGAGG ATGCCCTGCT GTGTCTCTGG 
GACCTGCAGG CACGCAAGTG GAAATTCGAG ATGAGCTACA CGAGTTCTTA CTGCAGAGGG 
GTCCAATGTG CTTGCTTCTC CAAGGATGAC AAGTATGTGT ACGTGGGCTT AAAGGATCGC 
TCTATACTTG TTTGGAGTGT GCTGGATGGC ACCCTGCTGA CAGTCCAGTT TGTCCATGCT 
GTGGTAAACA GAATCATCCC AACCACCAGT GGCTTCATTG CCCCCACCAG GCATGGTTAT 
CTGATCCGAG AGAATTTCCA GTGCCTTTCA GCAAAAGCCT CACCGCAGGA CCCTCTTAAG 
AACTTCAAGA AGGCAATGTG GATGGTCAAA TCCAGGCAGA GAGAGGAGCT AGTTGCAGCT 
GCAGGAGCTC CCCAGGACTT GGAGTCAGAA AGTGCCCAGG GAAATGAAAC CAAATCAAAC 
AAATGCTCAC AAGTGTGCCT GATAGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277284.1
CDS	1480..5769
Translation	MDSQFHMAGEASQSWLKAKEKQRGTSYMVAVIEWEIERSLLSSEDREQGATVFLREIQDLHKHILEDCALRMVDRLADGCLDADAQNLLSSLKSHITDMHPGVLKTHRLPWSRDLVNPKNKTHACYLKELGEQFVVRANHQVLTRLRELDTAGQELAWLYQEIRHHLWQSSEVIQTFCGRQELLARLGQQLRHDDSKQHTPLVLFGPPGIGKTALMCKLAEQMPRLLGHKTVTVLRLLGTSQMSSDARGLLKSICFQVCLAYGLPLPPAQVLDAHTRVVQFFHTLLHTVSCRNFESLVLLLDAMDDLDSVRHARRVPWLPLNCPPRVHLILSACSGALGVLDTLQRVLLDPEAYWEVKPLSGNQGQQMIQLLLAAARRTLSPVHTDLLWASLPECGNPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCTRLEQTHGQLLVAHVLGYIVSSRHGLSEAELKDVLSLDDEVLQDVYRDWTPPSKELLRFPPLLWVRLRRDLGYYLARRPVDGFTLLAIAHRQLVEVVRERYLSGSERAKRHGVLADFFSGTWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSHTVANLRKLKELPYHLLHSGRLEELKQEVLGSMSWISCRGISGGIEDLLDDFDLCAPHLDSPEVGLVREALQLCRPAVELRGMERSLLYTELLARLHFFATSHPALVGQLCQQAQSWFQLCAHPVLVPLGGFLQPPGGPLRATLSGCHKGITAMAWGVEEKLLVIGTQDGIMAVWDMEEQHVIHMLTGHTGEVRCVKIFAKGTLANSASKDYTLHLWNLLSGQEKFTIWDGGSKNPAEPQIWNLHVDEAHKVVYSASGSKINAWNLETAEPVFHILGDASDPWMCMAVLASQATLLTVSRDGVVSLWSSATGKLQGKQHMSSIKEETPTCAVSVQKQGKLVTGFSNGSISLVSSKGDRLLEKLPDAVRFLVVSEDESLLAAGFGRSVRIFLADSRGFRRFMAMDLEHEDMVETAVFGTENNLIITGSLDALIQVWSLSEQGTLLDILEGVGAPVSLLARGGALVASASPQSSSFKVWDLSDAHRSRVPAPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDLAEGEEQDSLDTSSEIRCLEVAEQRKLLFTGLVSGVVLVFPLNSRQDVICIPPPEARKAINCMSLSKCEDRLAIAYDNIVLVLDITSGDPCPVIDGPRYTFYTQLPETLSSVAILTDYRVVYSMTNGDLFLYECATSKAFPLETHRSRVACVEVSHKEQLVVSGSEDALLCLWDLQARKWKFEMSYTSSYCRGVQCACFSKDDKYVYVGLKDRSILVWSVLDGTLLTVQFVHAVVNRIIPTTSGFIAPTRHGYLIRENFQCLSAKASPQDPLKNFKKAMWMVKSRQREELVAAAGAPQDLESESAQGNETKSNKCSQVCLIV

Target ORF information:

RefSeq Version	NM_001290355.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290355.2

ORF Insert Sequence:

ATGGACTCAC AATTCCACAT GGCTGGGGAG GCCTCACAAT CATGGCTGAA GGCAAAGGAG 
AAGCAAAGAG GCACATCTTA CATGGTGGCA GTCATTGAGT GGGAGATAGA GCGGAGCCTG 
CTGAGCTCAG AGGACCGGGA ACAGGGAGCC ACCGTCTTCC TTAGAGAGAT CCAAGACCTC 
CACAAACACA TCCTTGAAGA CTGCGCCCTT AGGATGGTGG ACCGGCTCGC GGATGGCTGC 
CTGGACGCTG ATGCCCAGAA CCTTCTCAGC AGCCTCAAAA GTCACATCAC TGACATGCAC 
CCAGGGGTCC TCAAGACCCA CCGCCTGCCG TGGAGCCGCG ACTTGGTGAA CCCCAAGAAC 
AAGACTCACG CCTGCTACCT GAAGGAGCTG GGTGAGCAGT TTGTGGTGAG GGCCAATCAC 
CAGGTCCTCA CACGCCTCCG TGAGCTGGAT ACGGCCGGAC AGGAGTTGGC GTGGCTCTAC 
CAAGAGATCC GCCACCACCT TTGGCAGAGC TCGGAGGTCA TTCAGACCTT CTGCGGACGC 
CAGGAACTCC TGGCCCGGCT TGGGCAGCAG CTCAGGCACG ATGACAGCAA GCAGCACACC 
CCCCTGGTAC TCTTTGGGCC CCCAGGCATT GGAAAGACAG CCCTGATGTG CAAGCTGGCT 
GAGCAGATGC CAAGGCTGCT GGGGCACAAG ACAGTGACCG TCCTGCGGCT GCTGGGGACG 
TCACAAATGA GCTCAGATGC CCGTGGCCTG CTGAAGAGCA TCTGCTTCCA GGTGTGCCTG 
GCCTATGGGC TGCCCTTGCC CCCTGCCCAG GTTCTGGACG CCCACACCAG GGTGGTCCAG 
TTTTTCCATA CCCTCCTCCA CACTGTCTCT TGCAGAAACT TCGAGTCTCT CGTGCTCCTG 
CTGGATGCTA TGGATGACCT GGACTCTGTC CGCCATGCTC GGAGGGTTCC CTGGCTGCCT 
CTCAACTGCC CCCCGAGGGT GCACCTCATC CTCTCAGCTT GCTCGGGGGC ACTGGGGGTT 
TTGGACACCT TGCAGCGGGT GCTCCTGGAC CCGGAGGCCT ACTGGGAGGT GAAGCCCCTT 
TCCGGAAACC AAGGCCAGCA GATGATCCAA CTCCTGCTGG CAGCTGCAAG GAGGACGCTG 
AGCCCGGTGC ACACAGATTT GCTCTGGGCC AGCCTCCCAG AGTGTGGGAA CCCAGGGCGG 
CTGAGGCTGG CGTTTGAGGA AGCCCGGAAA TGGGCCTCTT TCACCGTGCC TGTCCCGCTG 
GCCACCACCG CAGAGGAAGC CACGCACCAA CTCTGCACCC GCCTGGAGCA GACACACGGG 
CAGCTCCTCG TGGCCCACGT GCTGGGCTAC ATTGTGTCTT CCCGACACGG TCTCTCGGAG 
GCGGAGCTGA AGGATGTTTT GTCCCTGGAC GACGAGGTCC TGCAGGATGT GTACCGAGAT 
TGGACCCCGC CCAGCAAGGA GCTGCTGCGC TTCCCGCCCC TGCTGTGGGT GCGGCTTCGT 
CGGGATCTGG GATACTACTT GGCCCGGCGG CCCGTGGATG GCTTCACCCT CCTGGCCATT 
GCCCACAGAC AGCTGGTCGA GGTGGTCCGT GAGCGCTACC TGTCAGGATC CGAGAGAGCC 
AAGAGGCATG GCGTCCTGGC CGACTTCTTC TCAGGGACCT GGAGCCAGGG TACCAAGAAG 
CTCATCACTC TGCCACTTGT GGGGAAACCA CTGAACTTGG ACCGAAAGGT GGCCCCGCAG 
CCTCTGTGGT TCTCACATAC GGTTGCAAAC CTGCGGAAGC TGAAGGAGTT GCCCTATCAC 
CTGCTTCACT CGGGCCGCCT GGAGGAGCTG AAACAGGAGG TTCTGGGCAG CATGAGCTGG 
ATTTCCTGCC GGGGCATCTC TGGGGGCATT GAAGACCTGC TGGATGACTT TGACCTGTGT 
GCCCCTCACC TGGACTCCCC TGAGGTTGGC CTGGTCCGTG AAGCCCTCCA GCTCTGCCGC 
CCTGCTGTGG AGCTCCGAGG CATGGAGAGG AGCCTCCTGT ACACAGAACT GCTGGCCAGA 
CTCCATTTCT TCGCCACCTC ACATCCAGCA CTGGTGGGAC AGCTATGCCA ACAGGCCCAG 
AGCTGGTTCC AGTTGTGCGC ACACCCTGTG CTGGTGCCCC TCGGAGGATT CCTCCAGCCC 
CCGGGAGGAC CCCTCCGGGC AACTCTCAGC GGCTGTCACA AAGGCATCAC CGCCATGGCA 
TGGGGTGTGG AGGAGAAGCT GCTGGTGATT GGCACCCAGG ATGGCATCAT GGCTGTGTGG 
GACATGGAAG AGCAGCATGT GATCCACATG CTAACTGGAC ACACAGGAGA GGTGAGGTGT 
GTGAAAATAT TTGCCAAAGG GACCCTCGCC AACTCTGCTT CAAAGGATTA CACGCTGCAC 
TTGTGGAACT TACTCTCTGG CCAGGAGAAA TTTACCATTT GGGATGGAGG CTCAAAAAAT 
CCCGCTGAAC CTCAGATCTG GAACCTTCAT GTGGATGAGG CACACAAAGT TGTGTATTCA 
GCATCTGGCT CAAAGATCAA TGCTTGGAAT CTGGAAACTG CAGAGCCGGT ATTCCATATC 
CTGGGAGATG CCTCTGATCC TTGGATGTGC ATGGCCGTGC TGGCCTCCCA GGCCACACTG 
CTGACAGTGT CCAGGGATGG TGTGGTCAGT CTGTGGAGCT CAGCTACGGG AAAACTTCAG 
GGGAAGCAAC ATATGTCCAG CATCAAAGAA GAAACACCTA CCTGTGCCGT CTCAGTCCAG 
AAGCAAGGAA AGCTTGTTAC CGGGTTTAGC AATGGCTCCA TCTCTTTGGT TTCCTCCAAA 
GGGGACAGAT TGCTGGAGAA GCTTCCAGAT GCTGTGAGGT TCCTGGTGGT CTCTGAAGAT 
GAGTCCCTCC TCGCCGCAGG CTTTGGAAGA TCGGTGCGGA TATTCTTGGC GGACTCGAGG 
GGCTTTCGCC GATTCATGGC CATGGATCTG GAACATGAAG ACATGGTGGA GACGGCTGTT 
TTTGGTACTG AGAACAACCT GATCATCACG GGGTCCCTTG ATGCGCTCAT TCAGGTGTGG 
AGTCTGTCAG AACAGGGGAC CCTTCTGGAC ATCCTGGAAG GCGTCGGGGC CCCCGTGAGC 
CTGCTGGCCC GCGGCGGGGC TTTGGTGGCA TCTGCTTCCC CACAGTCCTC ATCTTTCAAG 
GTCTGGGATC TCAGCGATGC TCATAGGTCC CGGGTGCCTG CACCATTTCT GGACCGCACC 
GGCCTCACCG CAGTGTCCCA CAATGGAAGC TACGTCTACT TCCCCAAAAT TGGGGACAAA 
AACAAAGTCA CTATTTGGGA CTTGGCAGAA GGCGAGGAAC AAGATTCCCT GGACACCTCC 
AGTGAGATCA GGTGTCTGGA GGTTGCTGAG CAGCGCAAGC TCCTATTTAC GGGCCTCGTG 
TCGGGGGTCG TCCTTGTGTT CCCCCTGAAT TCCAGGCAGG ACGTGATATG CATTCCCCCT 
CCCGAGGCCC GGAAAGCAAT CAACTGCATG TCCCTGAGCA AGTGCGAGGA CCGCCTGGCC 
ATCGCCTATG ACAACATCGT CCTGGTGCTG GACATCACCT CCGGGGACCC CTGCCCGGTC 
ATCGATGGGC CAAGATACAC CTTTTACACT CAGCTGCCCG AGACCCTCTC CAGCGTGGCC 
ATTCTGACGG ACTACCGCGT GGTCTACAGC ATGACCAATG GGGACCTCTT TCTTTACGAG 
TGTGCAACTT CCAAAGCGTT TCCCTTGGAG ACCCACAGGA GCCGAGTTGC CTGTGTGGAG 
GTCAGCCACA AGGAGCAGCT GGTGGTCAGC GGGTCTGAGG ATGCCCTGCT GTGTCTCTGG 
GACCTGCAGG CACGCAAGTG GAAATTCGAG ATGAGCTACA CGAGTTCTTA CTGCAGAGGG 
GTCCAATGTG CTTGCTTCTC CAAGGATGAC AAGTATGTGT ACGTGGGCTT AAAGGATCGC 
TCTATACTTG TTTGGAGTGT GCTGGATGGC ACCCTGCTGA CAGTCCAGTT TGTCCATGCT 
GTGGTAAACA GAATCATCCC AACCACCAGT GGCTTCATTG CCCCCACCAG GCATGGTTAT 
CTGATCCGAG AGAATTTCCA GTGCCTTTCA GCAAAAGCCT CACCGCAGGA CCCTCTTAAG 
AACTTCAAGA AGGCAATGTG GATGGTCAAA TCCAGGCAGA GAGAGGAGCT AGTTGCAGCT 
GCAGGAGCTC CCCAGGACTT GGAGTCAGAA AGTGCCCAGG GAAATGAAAC CAAATCAAAC 
AAATGCTCAC AAGTGTGCCT GATAGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( NM_001290355.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001277284.1 Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09297
Clone ID Related Accession (Same CDS sequence)	NM_001290355.2 , NM_001290355.3
Accession Version	NM_001290355.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	NACHT domain- and WD repeat-containing protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008737.11, BX648940.1, BC117698.1, AK090685.1 and CB242932.1. On Jun 2, 2019 this sequence version replaced NM_001290355.2. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, initiates translation at an alternate start codon and contains an alternate exon in the 3' coding region, resulting in a translational frameshift, compared to variant 1. It encodes isoform 2, which is shorter and has distinct N- and C-termini, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BX648940.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153427, SAMEA2158569 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTCAC AATTCCACAT GGCTGGGGAG GCCTCACAAT CATGGCTGAA GGCAAAGGAG 
AAGCAAAGAG GCACATCTTA CATGGTGGCA GTCATTGAGT GGGAGATAGA GCGGAGCCTG 
CTGAGCTCAG AGGACCGGGA ACAGGGAGCC ACCGTCTTCC TTAGAGAGAT CCAAGACCTC 
CACAAACACA TCCTTGAAGA CTGCGCCCTT AGGATGGTGG ACCGGCTCGC GGATGGCTGC 
CTGGACGCTG ATGCCCAGAA CCTTCTCAGC AGCCTCAAAA GTCACATCAC TGACATGCAC 
CCAGGGGTCC TCAAGACCCA CCGCCTGCCG TGGAGCCGCG ACTTGGTGAA CCCCAAGAAC 
AAGACTCACG CCTGCTACCT GAAGGAGCTG GGTGAGCAGT TTGTGGTGAG GGCCAATCAC 
CAGGTCCTCA CACGCCTCCG TGAGCTGGAT ACGGCCGGAC AGGAGTTGGC GTGGCTCTAC 
CAAGAGATCC GCCACCACCT TTGGCAGAGC TCGGAGGTCA TTCAGACCTT CTGCGGACGC 
CAGGAACTCC TGGCCCGGCT TGGGCAGCAG CTCAGGCACG ATGACAGCAA GCAGCACACC 
CCCCTGGTAC TCTTTGGGCC CCCAGGCATT GGAAAGACAG CCCTGATGTG CAAGCTGGCT 
GAGCAGATGC CAAGGCTGCT GGGGCACAAG ACAGTGACCG TCCTGCGGCT GCTGGGGACG 
TCACAAATGA GCTCAGATGC CCGTGGCCTG CTGAAGAGCA TCTGCTTCCA GGTGTGCCTG 
GCCTATGGGC TGCCCTTGCC CCCTGCCCAG GTTCTGGACG CCCACACCAG GGTGGTCCAG 
TTTTTCCATA CCCTCCTCCA CACTGTCTCT TGCAGAAACT TCGAGTCTCT CGTGCTCCTG 
CTGGATGCTA TGGATGACCT GGACTCTGTC CGCCATGCTC GGAGGGTTCC CTGGCTGCCT 
CTCAACTGCC CCCCGAGGGT GCACCTCATC CTCTCAGCTT GCTCGGGGGC ACTGGGGGTT 
TTGGACACCT TGCAGCGGGT GCTCCTGGAC CCGGAGGCCT ACTGGGAGGT GAAGCCCCTT 
TCCGGAAACC AAGGCCAGCA GATGATCCAA CTCCTGCTGG CAGCTGCAAG GAGGACGCTG 
AGCCCGGTGC ACACAGATTT GCTCTGGGCC AGCCTCCCAG AGTGTGGGAA CCCAGGGCGG 
CTGAGGCTGG CGTTTGAGGA AGCCCGGAAA TGGGCCTCTT TCACCGTGCC TGTCCCGCTG 
GCCACCACCG CAGAGGAAGC CACGCACCAA CTCTGCACCC GCCTGGAGCA GACACACGGG 
CAGCTCCTCG TGGCCCACGT GCTGGGCTAC ATTGTGTCTT CCCGACACGG TCTCTCGGAG 
GCGGAGCTGA AGGATGTTTT GTCCCTGGAC GACGAGGTCC TGCAGGATGT GTACCGAGAT 
TGGACCCCGC CCAGCAAGGA GCTGCTGCGC TTCCCGCCCC TGCTGTGGGT GCGGCTTCGT 
CGGGATCTGG GATACTACTT GGCCCGGCGG CCCGTGGATG GCTTCACCCT CCTGGCCATT 
GCCCACAGAC AGCTGGTCGA GGTGGTCCGT GAGCGCTACC TGTCAGGATC CGAGAGAGCC 
AAGAGGCATG GCGTCCTGGC CGACTTCTTC TCAGGGACCT GGAGCCAGGG TACCAAGAAG 
CTCATCACTC TGCCACTTGT GGGGAAACCA CTGAACTTGG ACCGAAAGGT GGCCCCGCAG 
CCTCTGTGGT TCTCACATAC GGTTGCAAAC CTGCGGAAGC TGAAGGAGTT GCCCTATCAC 
CTGCTTCACT CGGGCCGCCT GGAGGAGCTG AAACAGGAGG TTCTGGGCAG CATGAGCTGG 
ATTTCCTGCC GGGGCATCTC TGGGGGCATT GAAGACCTGC TGGATGACTT TGACCTGTGT 
GCCCCTCACC TGGACTCCCC TGAGGTTGGC CTGGTCCGTG AAGCCCTCCA GCTCTGCCGC 
CCTGCTGTGG AGCTCCGAGG CATGGAGAGG AGCCTCCTGT ACACAGAACT GCTGGCCAGA 
CTCCATTTCT TCGCCACCTC ACATCCAGCA CTGGTGGGAC AGCTATGCCA ACAGGCCCAG 
AGCTGGTTCC AGTTGTGCGC ACACCCTGTG CTGGTGCCCC TCGGAGGATT CCTCCAGCCC 
CCGGGAGGAC CCCTCCGGGC AACTCTCAGC GGCTGTCACA AAGGCATCAC CGCCATGGCA 
TGGGGTGTGG AGGAGAAGCT GCTGGTGATT GGCACCCAGG ATGGCATCAT GGCTGTGTGG 
GACATGGAAG AGCAGCATGT GATCCACATG CTAACTGGAC ACACAGGAGA GGTGAGGTGT 
GTGAAAATAT TTGCCAAAGG GACCCTCGCC AACTCTGCTT CAAAGGATTA CACGCTGCAC 
TTGTGGAACT TACTCTCTGG CCAGGAGAAA TTTACCATTT GGGATGGAGG CTCAAAAAAT 
CCCGCTGAAC CTCAGATCTG GAACCTTCAT GTGGATGAGG CACACAAAGT TGTGTATTCA 
GCATCTGGCT CAAAGATCAA TGCTTGGAAT CTGGAAACTG CAGAGCCGGT ATTCCATATC 
CTGGGAGATG CCTCTGATCC TTGGATGTGC ATGGCCGTGC TGGCCTCCCA GGCCACACTG 
CTGACAGTGT CCAGGGATGG TGTGGTCAGT CTGTGGAGCT CAGCTACGGG AAAACTTCAG 
GGGAAGCAAC ATATGTCCAG CATCAAAGAA GAAACACCTA CCTGTGCCGT CTCAGTCCAG 
AAGCAAGGAA AGCTTGTTAC CGGGTTTAGC AATGGCTCCA TCTCTTTGGT TTCCTCCAAA 
GGGGACAGAT TGCTGGAGAA GCTTCCAGAT GCTGTGAGGT TCCTGGTGGT CTCTGAAGAT 
GAGTCCCTCC TCGCCGCAGG CTTTGGAAGA TCGGTGCGGA TATTCTTGGC GGACTCGAGG 
GGCTTTCGCC GATTCATGGC CATGGATCTG GAACATGAAG ACATGGTGGA GACGGCTGTT 
TTTGGTACTG AGAACAACCT GATCATCACG GGGTCCCTTG ATGCGCTCAT TCAGGTGTGG 
AGTCTGTCAG AACAGGGGAC CCTTCTGGAC ATCCTGGAAG GCGTCGGGGC CCCCGTGAGC 
CTGCTGGCCC GCGGCGGGGC TTTGGTGGCA TCTGCTTCCC CACAGTCCTC ATCTTTCAAG 
GTCTGGGATC TCAGCGATGC TCATAGGTCC CGGGTGCCTG CACCATTTCT GGACCGCACC 
GGCCTCACCG CAGTGTCCCA CAATGGAAGC TACGTCTACT TCCCCAAAAT TGGGGACAAA 
AACAAAGTCA CTATTTGGGA CTTGGCAGAA GGCGAGGAAC AAGATTCCCT GGACACCTCC 
AGTGAGATCA GGTGTCTGGA GGTTGCTGAG CAGCGCAAGC TCCTATTTAC GGGCCTCGTG 
TCGGGGGTCG TCCTTGTGTT CCCCCTGAAT TCCAGGCAGG ACGTGATATG CATTCCCCCT 
CCCGAGGCCC GGAAAGCAAT CAACTGCATG TCCCTGAGCA AGTGCGAGGA CCGCCTGGCC 
ATCGCCTATG ACAACATCGT CCTGGTGCTG GACATCACCT CCGGGGACCC CTGCCCGGTC 
ATCGATGGGC CAAGATACAC CTTTTACACT CAGCTGCCCG AGACCCTCTC CAGCGTGGCC 
ATTCTGACGG ACTACCGCGT GGTCTACAGC ATGACCAATG GGGACCTCTT TCTTTACGAG 
TGTGCAACTT CCAAAGCGTT TCCCTTGGAG ACCCACAGGA GCCGAGTTGC CTGTGTGGAG 
GTCAGCCACA AGGAGCAGCT GGTGGTCAGC GGGTCTGAGG ATGCCCTGCT GTGTCTCTGG 
GACCTGCAGG CACGCAAGTG GAAATTCGAG ATGAGCTACA CGAGTTCTTA CTGCAGAGGG 
GTCCAATGTG CTTGCTTCTC CAAGGATGAC AAGTATGTGT ACGTGGGCTT AAAGGATCGC 
TCTATACTTG TTTGGAGTGT GCTGGATGGC ACCCTGCTGA CAGTCCAGTT TGTCCATGCT 
GTGGTAAACA GAATCATCCC AACCACCAGT GGCTTCATTG CCCCCACCAG GCATGGTTAT 
CTGATCCGAG AGAATTTCCA GTGCCTTTCA GCAAAAGCCT CACCGCAGGA CCCTCTTAAG 
AACTTCAAGA AGGCAATGTG GATGGTCAAA TCCAGGCAGA GAGAGGAGCT AGTTGCAGCT 
GCAGGAGCTC CCCAGGACTT GGAGTCAGAA AGTGCCCAGG GAAATGAAAC CAAATCAAAC 
AAATGCTCAC AAGTGTGCCT GATAGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277284.1
CDS	1609..5898
Translation	MDSQFHMAGEASQSWLKAKEKQRGTSYMVAVIEWEIERSLLSSEDREQGATVFLREIQDLHKHILEDCALRMVDRLADGCLDADAQNLLSSLKSHITDMHPGVLKTHRLPWSRDLVNPKNKTHACYLKELGEQFVVRANHQVLTRLRELDTAGQELAWLYQEIRHHLWQSSEVIQTFCGRQELLARLGQQLRHDDSKQHTPLVLFGPPGIGKTALMCKLAEQMPRLLGHKTVTVLRLLGTSQMSSDARGLLKSICFQVCLAYGLPLPPAQVLDAHTRVVQFFHTLLHTVSCRNFESLVLLLDAMDDLDSVRHARRVPWLPLNCPPRVHLILSACSGALGVLDTLQRVLLDPEAYWEVKPLSGNQGQQMIQLLLAAARRTLSPVHTDLLWASLPECGNPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCTRLEQTHGQLLVAHVLGYIVSSRHGLSEAELKDVLSLDDEVLQDVYRDWTPPSKELLRFPPLLWVRLRRDLGYYLARRPVDGFTLLAIAHRQLVEVVRERYLSGSERAKRHGVLADFFSGTWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSHTVANLRKLKELPYHLLHSGRLEELKQEVLGSMSWISCRGISGGIEDLLDDFDLCAPHLDSPEVGLVREALQLCRPAVELRGMERSLLYTELLARLHFFATSHPALVGQLCQQAQSWFQLCAHPVLVPLGGFLQPPGGPLRATLSGCHKGITAMAWGVEEKLLVIGTQDGIMAVWDMEEQHVIHMLTGHTGEVRCVKIFAKGTLANSASKDYTLHLWNLLSGQEKFTIWDGGSKNPAEPQIWNLHVDEAHKVVYSASGSKINAWNLETAEPVFHILGDASDPWMCMAVLASQATLLTVSRDGVVSLWSSATGKLQGKQHMSSIKEETPTCAVSVQKQGKLVTGFSNGSISLVSSKGDRLLEKLPDAVRFLVVSEDESLLAAGFGRSVRIFLADSRGFRRFMAMDLEHEDMVETAVFGTENNLIITGSLDALIQVWSLSEQGTLLDILEGVGAPVSLLARGGALVASASPQSSSFKVWDLSDAHRSRVPAPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDLAEGEEQDSLDTSSEIRCLEVAEQRKLLFTGLVSGVVLVFPLNSRQDVICIPPPEARKAINCMSLSKCEDRLAIAYDNIVLVLDITSGDPCPVIDGPRYTFYTQLPETLSSVAILTDYRVVYSMTNGDLFLYECATSKAFPLETHRSRVACVEVSHKEQLVVSGSEDALLCLWDLQARKWKFEMSYTSSYCRGVQCACFSKDDKYVYVGLKDRSILVWSVLDGTLLTVQFVHAVVNRIIPTTSGFIAPTRHGYLIRENFQCLSAKASPQDPLKNFKKAMWMVKSRQREELVAAAGAPQDLESESAQGNETKSNKCSQVCLIV

Target ORF information:

RefSeq Version	NM_001290355.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290355.3

ORF Insert Sequence:

ATGGACTCAC AATTCCACAT GGCTGGGGAG GCCTCACAAT CATGGCTGAA GGCAAAGGAG 
AAGCAAAGAG GCACATCTTA CATGGTGGCA GTCATTGAGT GGGAGATAGA GCGGAGCCTG 
CTGAGCTCAG AGGACCGGGA ACAGGGAGCC ACCGTCTTCC TTAGAGAGAT CCAAGACCTC 
CACAAACACA TCCTTGAAGA CTGCGCCCTT AGGATGGTGG ACCGGCTCGC GGATGGCTGC 
CTGGACGCTG ATGCCCAGAA CCTTCTCAGC AGCCTCAAAA GTCACATCAC TGACATGCAC 
CCAGGGGTCC TCAAGACCCA CCGCCTGCCG TGGAGCCGCG ACTTGGTGAA CCCCAAGAAC 
AAGACTCACG CCTGCTACCT GAAGGAGCTG GGTGAGCAGT TTGTGGTGAG GGCCAATCAC 
CAGGTCCTCA CACGCCTCCG TGAGCTGGAT ACGGCCGGAC AGGAGTTGGC GTGGCTCTAC 
CAAGAGATCC GCCACCACCT TTGGCAGAGC TCGGAGGTCA TTCAGACCTT CTGCGGACGC 
CAGGAACTCC TGGCCCGGCT TGGGCAGCAG CTCAGGCACG ATGACAGCAA GCAGCACACC 
CCCCTGGTAC TCTTTGGGCC CCCAGGCATT GGAAAGACAG CCCTGATGTG CAAGCTGGCT 
GAGCAGATGC CAAGGCTGCT GGGGCACAAG ACAGTGACCG TCCTGCGGCT GCTGGGGACG 
TCACAAATGA GCTCAGATGC CCGTGGCCTG CTGAAGAGCA TCTGCTTCCA GGTGTGCCTG 
GCCTATGGGC TGCCCTTGCC CCCTGCCCAG GTTCTGGACG CCCACACCAG GGTGGTCCAG 
TTTTTCCATA CCCTCCTCCA CACTGTCTCT TGCAGAAACT TCGAGTCTCT CGTGCTCCTG 
CTGGATGCTA TGGATGACCT GGACTCTGTC CGCCATGCTC GGAGGGTTCC CTGGCTGCCT 
CTCAACTGCC CCCCGAGGGT GCACCTCATC CTCTCAGCTT GCTCGGGGGC ACTGGGGGTT 
TTGGACACCT TGCAGCGGGT GCTCCTGGAC CCGGAGGCCT ACTGGGAGGT GAAGCCCCTT 
TCCGGAAACC AAGGCCAGCA GATGATCCAA CTCCTGCTGG CAGCTGCAAG GAGGACGCTG 
AGCCCGGTGC ACACAGATTT GCTCTGGGCC AGCCTCCCAG AGTGTGGGAA CCCAGGGCGG 
CTGAGGCTGG CGTTTGAGGA AGCCCGGAAA TGGGCCTCTT TCACCGTGCC TGTCCCGCTG 
GCCACCACCG CAGAGGAAGC CACGCACCAA CTCTGCACCC GCCTGGAGCA GACACACGGG 
CAGCTCCTCG TGGCCCACGT GCTGGGCTAC ATTGTGTCTT CCCGACACGG TCTCTCGGAG 
GCGGAGCTGA AGGATGTTTT GTCCCTGGAC GACGAGGTCC TGCAGGATGT GTACCGAGAT 
TGGACCCCGC CCAGCAAGGA GCTGCTGCGC TTCCCGCCCC TGCTGTGGGT GCGGCTTCGT 
CGGGATCTGG GATACTACTT GGCCCGGCGG CCCGTGGATG GCTTCACCCT CCTGGCCATT 
GCCCACAGAC AGCTGGTCGA GGTGGTCCGT GAGCGCTACC TGTCAGGATC CGAGAGAGCC 
AAGAGGCATG GCGTCCTGGC CGACTTCTTC TCAGGGACCT GGAGCCAGGG TACCAAGAAG 
CTCATCACTC TGCCACTTGT GGGGAAACCA CTGAACTTGG ACCGAAAGGT GGCCCCGCAG 
CCTCTGTGGT TCTCACATAC GGTTGCAAAC CTGCGGAAGC TGAAGGAGTT GCCCTATCAC 
CTGCTTCACT CGGGCCGCCT GGAGGAGCTG AAACAGGAGG TTCTGGGCAG CATGAGCTGG 
ATTTCCTGCC GGGGCATCTC TGGGGGCATT GAAGACCTGC TGGATGACTT TGACCTGTGT 
GCCCCTCACC TGGACTCCCC TGAGGTTGGC CTGGTCCGTG AAGCCCTCCA GCTCTGCCGC 
CCTGCTGTGG AGCTCCGAGG CATGGAGAGG AGCCTCCTGT ACACAGAACT GCTGGCCAGA 
CTCCATTTCT TCGCCACCTC ACATCCAGCA CTGGTGGGAC AGCTATGCCA ACAGGCCCAG 
AGCTGGTTCC AGTTGTGCGC ACACCCTGTG CTGGTGCCCC TCGGAGGATT CCTCCAGCCC 
CCGGGAGGAC CCCTCCGGGC AACTCTCAGC GGCTGTCACA AAGGCATCAC CGCCATGGCA 
TGGGGTGTGG AGGAGAAGCT GCTGGTGATT GGCACCCAGG ATGGCATCAT GGCTGTGTGG 
GACATGGAAG AGCAGCATGT GATCCACATG CTAACTGGAC ACACAGGAGA GGTGAGGTGT 
GTGAAAATAT TTGCCAAAGG GACCCTCGCC AACTCTGCTT CAAAGGATTA CACGCTGCAC 
TTGTGGAACT TACTCTCTGG CCAGGAGAAA TTTACCATTT GGGATGGAGG CTCAAAAAAT 
CCCGCTGAAC CTCAGATCTG GAACCTTCAT GTGGATGAGG CACACAAAGT TGTGTATTCA 
GCATCTGGCT CAAAGATCAA TGCTTGGAAT CTGGAAACTG CAGAGCCGGT ATTCCATATC 
CTGGGAGATG CCTCTGATCC TTGGATGTGC ATGGCCGTGC TGGCCTCCCA GGCCACACTG 
CTGACAGTGT CCAGGGATGG TGTGGTCAGT CTGTGGAGCT CAGCTACGGG AAAACTTCAG 
GGGAAGCAAC ATATGTCCAG CATCAAAGAA GAAACACCTA CCTGTGCCGT CTCAGTCCAG 
AAGCAAGGAA AGCTTGTTAC CGGGTTTAGC AATGGCTCCA TCTCTTTGGT TTCCTCCAAA 
GGGGACAGAT TGCTGGAGAA GCTTCCAGAT GCTGTGAGGT TCCTGGTGGT CTCTGAAGAT 
GAGTCCCTCC TCGCCGCAGG CTTTGGAAGA TCGGTGCGGA TATTCTTGGC GGACTCGAGG 
GGCTTTCGCC GATTCATGGC CATGGATCTG GAACATGAAG ACATGGTGGA GACGGCTGTT 
TTTGGTACTG AGAACAACCT GATCATCACG GGGTCCCTTG ATGCGCTCAT TCAGGTGTGG 
AGTCTGTCAG AACAGGGGAC CCTTCTGGAC ATCCTGGAAG GCGTCGGGGC CCCCGTGAGC 
CTGCTGGCCC GCGGCGGGGC TTTGGTGGCA TCTGCTTCCC CACAGTCCTC ATCTTTCAAG 
GTCTGGGATC TCAGCGATGC TCATAGGTCC CGGGTGCCTG CACCATTTCT GGACCGCACC 
GGCCTCACCG CAGTGTCCCA CAATGGAAGC TACGTCTACT TCCCCAAAAT TGGGGACAAA 
AACAAAGTCA CTATTTGGGA CTTGGCAGAA GGCGAGGAAC AAGATTCCCT GGACACCTCC 
AGTGAGATCA GGTGTCTGGA GGTTGCTGAG CAGCGCAAGC TCCTATTTAC GGGCCTCGTG 
TCGGGGGTCG TCCTTGTGTT CCCCCTGAAT TCCAGGCAGG ACGTGATATG CATTCCCCCT 
CCCGAGGCCC GGAAAGCAAT CAACTGCATG TCCCTGAGCA AGTGCGAGGA CCGCCTGGCC 
ATCGCCTATG ACAACATCGT CCTGGTGCTG GACATCACCT CCGGGGACCC CTGCCCGGTC 
ATCGATGGGC CAAGATACAC CTTTTACACT CAGCTGCCCG AGACCCTCTC CAGCGTGGCC 
ATTCTGACGG ACTACCGCGT GGTCTACAGC ATGACCAATG GGGACCTCTT TCTTTACGAG 
TGTGCAACTT CCAAAGCGTT TCCCTTGGAG ACCCACAGGA GCCGAGTTGC CTGTGTGGAG 
GTCAGCCACA AGGAGCAGCT GGTGGTCAGC GGGTCTGAGG ATGCCCTGCT GTGTCTCTGG 
GACCTGCAGG CACGCAAGTG GAAATTCGAG ATGAGCTACA CGAGTTCTTA CTGCAGAGGG 
GTCCAATGTG CTTGCTTCTC CAAGGATGAC AAGTATGTGT ACGTGGGCTT AAAGGATCGC 
TCTATACTTG TTTGGAGTGT GCTGGATGGC ACCCTGCTGA CAGTCCAGTT TGTCCATGCT 
GTGGTAAACA GAATCATCCC AACCACCAGT GGCTTCATTG CCCCCACCAG GCATGGTTAT 
CTGATCCGAG AGAATTTCCA GTGCCTTTCA GCAAAAGCCT CACCGCAGGA CCCTCTTAAG 
AACTTCAAGA AGGCAATGTG GATGGTCAAA TCCAGGCAGA GAGAGGAGCT AGTTGCAGCT 
GCAGGAGCTC CCCAGGACTT GGAGTCAGAA AGTGCCCAGG GAAATGAAAC CAAATCAAAC 
AAATGCTCAC AAGTGTGCCT GATAGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( NM_001347994.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001334923.1 Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant 3, mRNA.

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CloneID	OHu78811
Clone ID Related Accession (Same CDS sequence)	NM_001347994.1 , XM_017026669.1
Accession Version	NM_001347994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	NACHT domain- and WD repeat-containing protein 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR749794.1, BC117698.1, AC008737.11, AK090685.1 and CB242932.1. Transcript Variant: This variant (3) differs in the 5' UTR, uses a downstream start codon, and contains an alternate exon in its 3' coding region, resulting in a translational frameshift, compared to variant 1. It encodes isoform 3, which has a shorter N-terminus and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC117698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2153427 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGACC GGCTCGCGGA TGGCTGCCTG GACGCTGATG CCCAGAACCT TCTCAGCAGC 
CTCAAAAGTC ACATCACTGA CATGCACCCA GGGGTCCTCA AGACCCACCG CCTGCCGTGG 
AGCCGCGACT TGGTGAACCC CAAGAACAAG ACTCACGCCT GCTACCTGAA GGAGCTGGGT 
GAGCAGTTTG TGGTGAGGGC CAATCACCAG GTCCTCACAC GCCTCCGTGA GCTGGATACG 
GCCGGACAGG AGTTGGCGTG GCTCTACCAA GAGATCCGCC ACCACCTTTG GCAGAGCTCG 
GAGGTCATTC AGACCTTCTG CGGACGCCAG GAACTCCTGG CCCGGCTTGG GCAGCAGCTC 
AGGCACGATG ACAGCAAGCA GCACACCCCC CTGGTACTCT TTGGGCCCCC AGGCATTGGA 
AAGACAGCCC TGATGTGCAA GCTGGCTGAG CAGATGCCAA GGCTGCTGGG GCACAAGACA 
GTGACCGTCC TGCGGCTGCT GGGGACGTCA CAAATGAGCT CAGATGCCCG TGGCCTGCTG 
AAGAGCATCT GCTTCCAGGT GTGCCTGGCC TATGGGCTGC CCTTGCCCCC TGCCCAGGTT 
CTGGACGCCC ACACCAGGGT GGTCCAGTTT TTCCATACCC TCCTCCACAC TGTCTCTTGC 
AGAAACTTCG AGTCTCTCGT GCTCCTGCTG GATGCTATGG ATGACCTGGA CTCTGTCCGC 
CATGCTCGGA GGGTTCCCTG GCTGCCTCTC AACTGCCCCC CGAGGGTGCA CCTCATCCTC 
TCAGCTTGCT CGGGGGCACT GGGGGTTTTG GACACCTTGC AGCGGGTGCT CCTGGACCCG 
GAGGCCTACT GGGAGGTGAA GCCCCTTTCC GGAAACCAAG GCCAGCAGAT GATCCAACTC 
CTGCTGGCAG CTGCAAGGAG GACGCTGAGC CCGGTGCACA CAGATTTGCT CTGGGCCAGC 
CTCCCAGAGT GTGGGAACCC AGGGCGGCTG AGGCTGGCGT TTGAGGAAGC CCGGAAATGG 
GCCTCTTTCA CCGTGCCTGT CCCGCTGGCC ACCACCGCAG AGGAAGCCAC GCACCAACTC 
TGCACCCGCC TGGAGCAGAC ACACGGGCAG CTCCTCGTGG CCCACGTGCT GGGCTACATT 
GTGTCTTCCC GACACGGTCT CTCGGAGGCG GAGCTGAAGG ATGTTTTGTC CCTGGACGAC 
GAGGTCCTGC AGGATGTGTA CCGAGATTGG ACCCCGCCCA GCAAGGAGCT GCTGCGCTTC 
CCGCCCCTGC TGTGGGTGCG GCTTCGTCGG GATCTGGGAT ACTACTTGGC CCGGCGGCCC 
GTGGATGGCT TCACCCTCCT GGCCATTGCC CACAGACAGC TGGTCGAGGT GGTCCGTGAG 
CGCTACCTGT CAGGATCCGA GAGAGCCAAG AGGCATGGCG TCCTGGCCGA CTTCTTCTCA 
GGGACCTGGA GCCAGGGTAC CAAGAAGCTC ATCACTCTGC CACTTGTGGG GAAACCACTG 
AACTTGGACC GAAAGGTGGC CCCGCAGCCT CTGTGGTTCT CACATACGGT TGCAAACCTG 
CGGAAGCTGA AGGAGTTGCC CTATCACCTG CTTCACTCGG GCCGCCTGGA GGAGCTGAAA 
CAGGAGGTTC TGGGCAGCAT GAGCTGGATT TCCTGCCGGG GCATCTCTGG GGGCATTGAA 
GACCTGCTGG ATGACTTTGA CCTGTGTGCC CCTCACCTGG ACTCCCCTGA GGTTGGCCTG 
GTCCGTGAAG CCCTCCAGCT CTGCCGCCCT GCTGTGGAGC TCCGAGGCAT GGAGAGGAGC 
CTCCTGTACA CAGAACTGCT GGCCAGACTC CATTTCTTCG CCACCTCACA TCCAGCACTG 
GTGGGACAGC TATGCCAACA GGCCCAGAGC TGGTTCCAGT TGTGCGCACA CCCTGTGCTG 
GTGCCCCTCG GAGGATTCCT CCAGCCCCCG GGAGGACCCC TCCGGGCAAC TCTCAGCGGC 
TGTCACAAAG GCATCACCGC CATGGCATGG GGTGTGGAGG AGAAGCTGCT GGTGATTGGC 
ACCCAGGATG GCATCATGGC TGTGTGGGAC ATGGAAGAGC AGCATGTGAT CCACATGCTA 
ACTGGACACA CAGGAGAGGT GAGGTGTGTG AAAATATTTG CCAAAGGGAC CCTCGCCAAC 
TCTGCTTCAA AGGATTACAC GCTGCACTTG TGGAACTTAC TCTCTGGCCA GGAGAAATTT 
ACCATTTGGG ATGGAGGCTC AAAAAATCCC GCTGAACCTC AGATCTGGAA CCTTCATGTG 
GATGAGGCAC ACAAAGTTGT GTATTCAGCA TCTGGCTCAA AGATCAATGC TTGGAATCTG 
GAAACTGCAG AGCCGGTATT CCATATCCTG GGAGATGCCT CTGATCCTTG GATGTGCATG 
GCCGTGCTGG CCTCCCAGGC CACACTGCTG ACAGTGTCCA GGGATGGTGT GGTCAGTCTG 
TGGAGCTCAG CTACGGGAAA ACTTCAGGGG AAGCAACATA TGTCCAGCAT CAAAGAAGAA 
ACACCTACCT GTGCCGTCTC AGTCCAGAAG CAAGGAAAGC TTGTTACCGG GTTTAGCAAT 
GGCTCCATCT CTTTGGTTTC CTCCAAAGGG GACAGATTGC TGGAGAAGCT TCCAGATGCT 
GTGAGGTTCC TGGTGGTCTC TGAAGATGAG TCCCTCCTCG CCGCAGGCTT TGGAAGATCG 
GTGCGGATAT TCTTGGCGGA CTCGAGGGGC TTTCGCCGAT TCATGGCCAT GGATCTGGAA 
CATGAAGACA TGGTGGAGAC GGCTGTTTTT GGTACTGAGA ACAACCTGAT CATCACGGGG 
TCCCTTGATG CGCTCATTCA GGTGTGGAGT CTGTCAGAAC AGGGGACCCT TCTGGACATC 
CTGGAAGGCG TCGGGGCCCC CGTGAGCCTG CTGGCCCGCG GCGGGGCTTT GGTGGCATCT 
GCTTCCCCAC AGTCCTCATC TTTCAAGGTC TGGGATCTCA GCGATGCTCA TAGGTCCCGG 
GTGCCTGCAC CATTTCTGGA CCGCACCGGC CTCACCGCAG TGTCCCACAA TGGAAGCTAC 
GTCTACTTCC CCAAAATTGG GGACAAAAAC AAAGTCACTA TTTGGGACTT GGCAGAAGGC 
GAGGAACAAG ATTCCCTGGA CACCTCCAGT GAGATCAGGT GTCTGGAGGT TGCTGAGCAG 
CGCAAGCTCC TATTTACGGG CCTCGTGTCG GGGGTCGTCC TTGTGTTCCC CCTGAATTCC 
AGGCAGGACG TGATATGCAT TCCCCCTCCC GAGGCCCGGA AAGCAATCAA CTGCATGTCC 
CTGAGCAAGT GCGAGGACCG CCTGGCCATC GCCTATGACA ACATCGTCCT GGTGCTGGAC 
ATCACCTCCG GGGACCCCTG CCCGGTCATC GATGGGCCAA GATACACCTT TTACACTCAG 
CTGCCCGAGA CCCTCTCCAG CGTGGCCATT CTGACGGACT ACCGCGTGGT CTACAGCATG 
ACCAATGGGG ACCTCTTTCT TTACGAGTGT GCAACTTCCA AAGCGTTTCC CTTGGAGACC 
CACAGGAGCC GAGTTGCCTG TGTGGAGGTC AGCCACAAGG AGCAGCTGGT GGTCAGCGGG 
TCTGAGGATG CCCTGCTGTG TCTCTGGGAC CTGCAGGCAC GCAAGTGGAA ATTCGAGATG 
AGCTACACGA GTTCTTACTG CAGAGGGGTC CAATGTGCTT GCTTCTCCAA GGATGACAAG 
TATGTGTACG TGGGCTTAAA GGATCGCTCT ATACTTGTTT GGAGTGTGCT GGATGGCACC 
CTGCTGACAG TCCAGTTTGT CCATGCTGTG GTAAACAGAA TCATCCCAAC CACCAGTGGC 
TTCATTGCCC CCACCAGGCA TGGTTATCTG ATCCGAGAGA ATTTCCAGTG CCTTTCAGCA 
AAAGCCTCAC CGCAGGACCC TCTTAAGAAC TTCAAGAAGG CAATGTGGAT GGTCAAATCC 
AGGCAGAGAG AGGAGCTAGT TGCAGCTGCA GGAGCTCCCC AGGACTTGGA GTCAGAAAGT 
GCCCAGGGAA ATGAAACCAA ATCAAACAAA TGCTCACAAG TGTGCCTGAT AGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334923.1
CDS	692..4768
Translation	MVDRLADGCLDADAQNLLSSLKSHITDMHPGVLKTHRLPWSRDLVNPKNKTHACYLKELGEQFVVRANHQVLTRLRELDTAGQELAWLYQEIRHHLWQSSEVIQTFCGRQELLARLGQQLRHDDSKQHTPLVLFGPPGIGKTALMCKLAEQMPRLLGHKTVTVLRLLGTSQMSSDARGLLKSICFQVCLAYGLPLPPAQVLDAHTRVVQFFHTLLHTVSCRNFESLVLLLDAMDDLDSVRHARRVPWLPLNCPPRVHLILSACSGALGVLDTLQRVLLDPEAYWEVKPLSGNQGQQMIQLLLAAARRTLSPVHTDLLWASLPECGNPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCTRLEQTHGQLLVAHVLGYIVSSRHGLSEAELKDVLSLDDEVLQDVYRDWTPPSKELLRFPPLLWVRLRRDLGYYLARRPVDGFTLLAIAHRQLVEVVRERYLSGSERAKRHGVLADFFSGTWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSHTVANLRKLKELPYHLLHSGRLEELKQEVLGSMSWISCRGISGGIEDLLDDFDLCAPHLDSPEVGLVREALQLCRPAVELRGMERSLLYTELLARLHFFATSHPALVGQLCQQAQSWFQLCAHPVLVPLGGFLQPPGGPLRATLSGCHKGITAMAWGVEEKLLVIGTQDGIMAVWDMEEQHVIHMLTGHTGEVRCVKIFAKGTLANSASKDYTLHLWNLLSGQEKFTIWDGGSKNPAEPQIWNLHVDEAHKVVYSASGSKINAWNLETAEPVFHILGDASDPWMCMAVLASQATLLTVSRDGVVSLWSSATGKLQGKQHMSSIKEETPTCAVSVQKQGKLVTGFSNGSISLVSSKGDRLLEKLPDAVRFLVVSEDESLLAAGFGRSVRIFLADSRGFRRFMAMDLEHEDMVETAVFGTENNLIITGSLDALIQVWSLSEQGTLLDILEGVGAPVSLLARGGALVASASPQSSSFKVWDLSDAHRSRVPAPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDLAEGEEQDSLDTSSEIRCLEVAEQRKLLFTGLVSGVVLVFPLNSRQDVICIPPPEARKAINCMSLSKCEDRLAIAYDNIVLVLDITSGDPCPVIDGPRYTFYTQLPETLSSVAILTDYRVVYSMTNGDLFLYECATSKAFPLETHRSRVACVEVSHKEQLVVSGSEDALLCLWDLQARKWKFEMSYTSSYCRGVQCACFSKDDKYVYVGLKDRSILVWSVLDGTLLTVQFVHAVVNRIIPTTSGFIAPTRHGYLIRENFQCLSAKASPQDPLKNFKKAMWMVKSRQREELVAAAGAPQDLESESAQGNETKSNKCSQVCLIV

Target ORF information:

RefSeq Version	NM_001347994.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347994.1

ORF Insert Sequence:

ATGGTGGACC GGCTCGCGGA TGGCTGCCTG GACGCTGATG CCCAGAACCT TCTCAGCAGC 
CTCAAAAGTC ACATCACTGA CATGCACCCA GGGGTCCTCA AGACCCACCG CCTGCCGTGG 
AGCCGCGACT TGGTGAACCC CAAGAACAAG ACTCACGCCT GCTACCTGAA GGAGCTGGGT 
GAGCAGTTTG TGGTGAGGGC CAATCACCAG GTCCTCACAC GCCTCCGTGA GCTGGATACG 
GCCGGACAGG AGTTGGCGTG GCTCTACCAA GAGATCCGCC ACCACCTTTG GCAGAGCTCG 
GAGGTCATTC AGACCTTCTG CGGACGCCAG GAACTCCTGG CCCGGCTTGG GCAGCAGCTC 
AGGCACGATG ACAGCAAGCA GCACACCCCC CTGGTACTCT TTGGGCCCCC AGGCATTGGA 
AAGACAGCCC TGATGTGCAA GCTGGCTGAG CAGATGCCAA GGCTGCTGGG GCACAAGACA 
GTGACCGTCC TGCGGCTGCT GGGGACGTCA CAAATGAGCT CAGATGCCCG TGGCCTGCTG 
AAGAGCATCT GCTTCCAGGT GTGCCTGGCC TATGGGCTGC CCTTGCCCCC TGCCCAGGTT 
CTGGACGCCC ACACCAGGGT GGTCCAGTTT TTCCATACCC TCCTCCACAC TGTCTCTTGC 
AGAAACTTCG AGTCTCTCGT GCTCCTGCTG GATGCTATGG ATGACCTGGA CTCTGTCCGC 
CATGCTCGGA GGGTTCCCTG GCTGCCTCTC AACTGCCCCC CGAGGGTGCA CCTCATCCTC 
TCAGCTTGCT CGGGGGCACT GGGGGTTTTG GACACCTTGC AGCGGGTGCT CCTGGACCCG 
GAGGCCTACT GGGAGGTGAA GCCCCTTTCC GGAAACCAAG GCCAGCAGAT GATCCAACTC 
CTGCTGGCAG CTGCAAGGAG GACGCTGAGC CCGGTGCACA CAGATTTGCT CTGGGCCAGC 
CTCCCAGAGT GTGGGAACCC AGGGCGGCTG AGGCTGGCGT TTGAGGAAGC CCGGAAATGG 
GCCTCTTTCA CCGTGCCTGT CCCGCTGGCC ACCACCGCAG AGGAAGCCAC GCACCAACTC 
TGCACCCGCC TGGAGCAGAC ACACGGGCAG CTCCTCGTGG CCCACGTGCT GGGCTACATT 
GTGTCTTCCC GACACGGTCT CTCGGAGGCG GAGCTGAAGG ATGTTTTGTC CCTGGACGAC 
GAGGTCCTGC AGGATGTGTA CCGAGATTGG ACCCCGCCCA GCAAGGAGCT GCTGCGCTTC 
CCGCCCCTGC TGTGGGTGCG GCTTCGTCGG GATCTGGGAT ACTACTTGGC CCGGCGGCCC 
GTGGATGGCT TCACCCTCCT GGCCATTGCC CACAGACAGC TGGTCGAGGT GGTCCGTGAG 
CGCTACCTGT CAGGATCCGA GAGAGCCAAG AGGCATGGCG TCCTGGCCGA CTTCTTCTCA 
GGGACCTGGA GCCAGGGTAC CAAGAAGCTC ATCACTCTGC CACTTGTGGG GAAACCACTG 
AACTTGGACC GAAAGGTGGC CCCGCAGCCT CTGTGGTTCT CACATACGGT TGCAAACCTG 
CGGAAGCTGA AGGAGTTGCC CTATCACCTG CTTCACTCGG GCCGCCTGGA GGAGCTGAAA 
CAGGAGGTTC TGGGCAGCAT GAGCTGGATT TCCTGCCGGG GCATCTCTGG GGGCATTGAA 
GACCTGCTGG ATGACTTTGA CCTGTGTGCC CCTCACCTGG ACTCCCCTGA GGTTGGCCTG 
GTCCGTGAAG CCCTCCAGCT CTGCCGCCCT GCTGTGGAGC TCCGAGGCAT GGAGAGGAGC 
CTCCTGTACA CAGAACTGCT GGCCAGACTC CATTTCTTCG CCACCTCACA TCCAGCACTG 
GTGGGACAGC TATGCCAACA GGCCCAGAGC TGGTTCCAGT TGTGCGCACA CCCTGTGCTG 
GTGCCCCTCG GAGGATTCCT CCAGCCCCCG GGAGGACCCC TCCGGGCAAC TCTCAGCGGC 
TGTCACAAAG GCATCACCGC CATGGCATGG GGTGTGGAGG AGAAGCTGCT GGTGATTGGC 
ACCCAGGATG GCATCATGGC TGTGTGGGAC ATGGAAGAGC AGCATGTGAT CCACATGCTA 
ACTGGACACA CAGGAGAGGT GAGGTGTGTG AAAATATTTG CCAAAGGGAC CCTCGCCAAC 
TCTGCTTCAA AGGATTACAC GCTGCACTTG TGGAACTTAC TCTCTGGCCA GGAGAAATTT 
ACCATTTGGG ATGGAGGCTC AAAAAATCCC GCTGAACCTC AGATCTGGAA CCTTCATGTG 
GATGAGGCAC ACAAAGTTGT GTATTCAGCA TCTGGCTCAA AGATCAATGC TTGGAATCTG 
GAAACTGCAG AGCCGGTATT CCATATCCTG GGAGATGCCT CTGATCCTTG GATGTGCATG 
GCCGTGCTGG CCTCCCAGGC CACACTGCTG ACAGTGTCCA GGGATGGTGT GGTCAGTCTG 
TGGAGCTCAG CTACGGGAAA ACTTCAGGGG AAGCAACATA TGTCCAGCAT CAAAGAAGAA 
ACACCTACCT GTGCCGTCTC AGTCCAGAAG CAAGGAAAGC TTGTTACCGG GTTTAGCAAT 
GGCTCCATCT CTTTGGTTTC CTCCAAAGGG GACAGATTGC TGGAGAAGCT TCCAGATGCT 
GTGAGGTTCC TGGTGGTCTC TGAAGATGAG TCCCTCCTCG CCGCAGGCTT TGGAAGATCG 
GTGCGGATAT TCTTGGCGGA CTCGAGGGGC TTTCGCCGAT TCATGGCCAT GGATCTGGAA 
CATGAAGACA TGGTGGAGAC GGCTGTTTTT GGTACTGAGA ACAACCTGAT CATCACGGGG 
TCCCTTGATG CGCTCATTCA GGTGTGGAGT CTGTCAGAAC AGGGGACCCT TCTGGACATC 
CTGGAAGGCG TCGGGGCCCC CGTGAGCCTG CTGGCCCGCG GCGGGGCTTT GGTGGCATCT 
GCTTCCCCAC AGTCCTCATC TTTCAAGGTC TGGGATCTCA GCGATGCTCA TAGGTCCCGG 
GTGCCTGCAC CATTTCTGGA CCGCACCGGC CTCACCGCAG TGTCCCACAA TGGAAGCTAC 
GTCTACTTCC CCAAAATTGG GGACAAAAAC AAAGTCACTA TTTGGGACTT GGCAGAAGGC 
GAGGAACAAG ATTCCCTGGA CACCTCCAGT GAGATCAGGT GTCTGGAGGT TGCTGAGCAG 
CGCAAGCTCC TATTTACGGG CCTCGTGTCG GGGGTCGTCC TTGTGTTCCC CCTGAATTCC 
AGGCAGGACG TGATATGCAT TCCCCCTCCC GAGGCCCGGA AAGCAATCAA CTGCATGTCC 
CTGAGCAAGT GCGAGGACCG CCTGGCCATC GCCTATGACA ACATCGTCCT GGTGCTGGAC 
ATCACCTCCG GGGACCCCTG CCCGGTCATC GATGGGCCAA GATACACCTT TTACACTCAG 
CTGCCCGAGA CCCTCTCCAG CGTGGCCATT CTGACGGACT ACCGCGTGGT CTACAGCATG 
ACCAATGGGG ACCTCTTTCT TTACGAGTGT GCAACTTCCA AAGCGTTTCC CTTGGAGACC 
CACAGGAGCC GAGTTGCCTG TGTGGAGGTC AGCCACAAGG AGCAGCTGGT GGTCAGCGGG 
TCTGAGGATG CCCTGCTGTG TCTCTGGGAC CTGCAGGCAC GCAAGTGGAA ATTCGAGATG 
AGCTACACGA GTTCTTACTG CAGAGGGGTC CAATGTGCTT GCTTCTCCAA GGATGACAAG 
TATGTGTACG TGGGCTTAAA GGATCGCTCT ATACTTGTTT GGAGTGTGCT GGATGGCACC 
CTGCTGACAG TCCAGTTTGT CCATGCTGTG GTAAACAGAA TCATCCCAAC CACCAGTGGC 
TTCATTGCCC CCACCAGGCA TGGTTATCTG ATCCGAGAGA ATTTCCAGTG CCTTTCAGCA 
AAAGCCTCAC CGCAGGACCC TCTTAAGAAC TTCAAGAAGG CAATGTGGAT GGTCAAATCC 
AGGCAGAGAG AGGAGCTAGT TGCAGCTGCA GGAGCTCCCC AGGACTTGGA GTCAGAAAGT 
GCCCAGGGAA ATGAAACCAA ATCAAACAAA TGCTCACAAG TGTGCCTGAT AGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_011527929.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526231.1 Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.

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CloneID	OHu78810
Clone ID Related Accession (Same CDS sequence)	XM_011527929.1 , XM_011527931.2 , XM_011527930.2
Accession Version	XM_011527929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGTTTCCT CCAAAGGGGA CAGATTGCTG 
GAGAAGCTTC CAGATGCTGT GAGGTTCCTG GTGGTCTCTG AAGATGAGTC CCTCCTCGCC 
GCAGGCTTTG GAAGATCGGT GCGGATATTC TTGGCGGACT CGAGGGGCTT TCGCCGATTC 
ATGGCCATGG ATCTGGAACA TGAAGACATG GTGGAGACGG CTGTTTTTGG TACTGAGAAC 
AACCTGATCA TCACGGGGTC CCTTGATGCG CTCATTCAGG TGTGGAGTCT GTCAGAACAG 
GGGACCCTTC TGGACATCCT GGAAGGCGTC GGGGCCCCCG TGAGCCTGCT GGCCCGCGGC 
GGGGCTTTGG TGGCATCTGC TTCCCCACAG TCCTCATCTT TCAAGGTCTG GGATCTCAGC 
GATGCTCATA GGTCCCGGGT GCCTGCACCA TTTCTGGACC GCACCGGCCT CACCGCAGTG 
TCCCACAATG GAAGCTACGT CTACTTCCCC AAAATTGGGG ACAAAAACAA AGTCACTATT 
TGGGACTTGG CAGAAGGCGA GGAACAAGAT TCCCTGGACA CCTCCAGTGA GATCAGGTGT 
CTGGAGGTTG CTGAGCAGCG CAAGCTCCTA TTTACGGGCC TCGTGTCGGG GGTCGTCCTT 
GTGTTCCCCC TGAATTCCAG GCAGGACGTG ATATGCATTC CCCCTCCCGA GGCCCGGAAA 
GCAATCAACT GCATGTCCCT GAGCAAGTGC GAGGACCGCC TGGCCATCGC CTATGACAAC 
ATCGTCCTGG TGCTGGACAT CACCTCCGGG GACCCCTGCC CGGTCATCGA TGGGCCAAGA 
TACACCTTTT ACACTCAGCT GCCCGAGACC CTCTCCAGCG TGGCCATTCT GACGGACTAC 
CGCGTGGTCT ACAGCATGAC CAATGGGGAC CTCTTTCTTT ACGAGTGTGC AACTTCCAAA 
GCGTTTCCCT TGGAGACCCA CAGGAGCCGA GTTGCCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGTGG TCAGCGGGTC TGAGGATGCC CTGCTGTGTC TCTGGGACCT GCAGGCACGC 
AAGTGGAAAT TCGAGATGAG CTACACGAGT TCTTACTGCA GAGGGGTCCA ATGTGCTTGC 
TTCTCCAAGG ATGACAAGTA TGTGTACGTG GGCTTAAAGG ATCGCTCTAT ACTTGTTTGG 
AGTGTGCTGG ATGGCACCCT GCTGACAGTC CAGTTTGTCC ATGCTGTGGT AAACAGAATC 
ATCCCAACCA CCAGTGGCTT CATTGCCCCC ACCAGGCATG GTTATCTGAT CCGAGAGAAT 
TTCCAGTGCC TTTCAGCAAA AGCCTCACCG CAGGACCCTC TTAAGAACTT CAAGAAGGCA 
ATGTGGATGG TCAAATCCAG GCAGAGAGAG GAGCTAGTTG CAGCTGCAGG AGCTCCCCAG 
GACTTGGAGT CAGAAAGTGC CCAGGGAAAT GAAACCAAAT CAAACAAATG CTCACAAGTG 
TGCCTGATAG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526231.1
CDS	607..5301
Translation	MQRGKPCRALPTLKCQTFCQRHGLMFEVVDLRWGIRNIEATDHLTTELCLEEVDRCWKTSIGPAFVALIGDQYGPCLIPSRIDEKEWEVLRDHLTARPSDLELVARYFQRDENAFPPTYVLQAPGTGEACEPEEATLTSVLRSGAQEARRLGLITQEQWQHYHRSVIEWEIERSLLSSEDREQGATVFLREIQDLHKHILEDCALRMVDRLADGCLDADAQNLLSSLKSHITDMHPGVLKTHRLPWSRDLVNPKNKTHACYLKELGEQFVVRANHQVLTRLRELDTAGQELAWLYQEIRHHLWQSSEVIQTFCGRQELLARLGQQLRHDDSKQHTPLVLFGPPGIGKTALMCKLAEQMPRLLGHKTVTVLRLLGTSQMSSDARGLLKSICFQVCLAYGLPLPPAQVLDAHTRVVQFFHTLLHTVSCRNFESLVLLLDAMDDLDSVRHARRVPWLPLNCPPRVHLILSACSGALGVLDTLQRVLLDPEAYWEVKPLSGNQGQQMIQLLLAAARRTLSPVHTDLLWASLPECGNPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCTRLEQTHGQLLVAHVLGYIVSSRHGLSEAELKDVLSLDDEVLQDVYRDWTPPSKELLRFPPLLWVRLRRDLGYYLARRPVDGFTLLAIAHRQLVEVVRERYLSGSERAKRHGVLADFFSGTWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSHTVANLRKLKELPYHLLHSGRLEELKQEVLGSMSWISCRGISGGIEDLLDDFDLCAPHLDSPEVGLVREALQLCRPAVELRGMERSLLYTELLARLHFFATSHPALVGQLCQQAQSWFQLCAHPVLVPLGGFLQPPGGPLRATLSGCHKGITAMAWGVEEKLLVIGTQDGIMAVWDMEEQHVIHMLTGHTGEVRCVKIFAKGTLANSASKDYTLHLWNLLSGQEKFTIWDGGSKNPAEPQIWNLHVDEAHKVVYSASGSKINAWNLETAEPVFHILGDASDPWMCMAVLASQATLLTVSRDGVVSLWSSATGKLQGKQHMSSIKEETPTCAVSVQKQGKLVTGFSNGSISLVSSKGDRLLEKLPDAVRFLVVSEDESLLAAGFGRSVRIFLADSRGFRRFMAMDLEHEDMVETAVFGTENNLIITGSLDALIQVWSLSEQGTLLDILEGVGAPVSLLARGGALVASASPQSSSFKVWDLSDAHRSRVPAPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDLAEGEEQDSLDTSSEIRCLEVAEQRKLLFTGLVSGVVLVFPLNSRQDVICIPPPEARKAINCMSLSKCEDRLAIAYDNIVLVLDITSGDPCPVIDGPRYTFYTQLPETLSSVAILTDYRVVYSMTNGDLFLYECATSKAFPLETHRSRVACVEVSHKEQLVVSGSEDALLCLWDLQARKWKFEMSYTSSYCRGVQCACFSKDDKYVYVGLKDRSILVWSVLDGTLLTVQFVHAVVNRIIPTTSGFIAPTRHGYLIRENFQCLSAKASPQDPLKNFKKAMWMVKSRQREELVAAAGAPQDLESESAQGNETKSNKCSQVCLIV

Target ORF information:

RefSeq Version	XM_011527929.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527929.1

ORF Insert Sequence:

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGTTTCCT CCAAAGGGGA CAGATTGCTG 
GAGAAGCTTC CAGATGCTGT GAGGTTCCTG GTGGTCTCTG AAGATGAGTC CCTCCTCGCC 
GCAGGCTTTG GAAGATCGGT GCGGATATTC TTGGCGGACT CGAGGGGCTT TCGCCGATTC 
ATGGCCATGG ATCTGGAACA TGAAGACATG GTGGAGACGG CTGTTTTTGG TACTGAGAAC 
AACCTGATCA TCACGGGGTC CCTTGATGCG CTCATTCAGG TGTGGAGTCT GTCAGAACAG 
GGGACCCTTC TGGACATCCT GGAAGGCGTC GGGGCCCCCG TGAGCCTGCT GGCCCGCGGC 
GGGGCTTTGG TGGCATCTGC TTCCCCACAG TCCTCATCTT TCAAGGTCTG GGATCTCAGC 
GATGCTCATA GGTCCCGGGT GCCTGCACCA TTTCTGGACC GCACCGGCCT CACCGCAGTG 
TCCCACAATG GAAGCTACGT CTACTTCCCC AAAATTGGGG ACAAAAACAA AGTCACTATT 
TGGGACTTGG CAGAAGGCGA GGAACAAGAT TCCCTGGACA CCTCCAGTGA GATCAGGTGT 
CTGGAGGTTG CTGAGCAGCG CAAGCTCCTA TTTACGGGCC TCGTGTCGGG GGTCGTCCTT 
GTGTTCCCCC TGAATTCCAG GCAGGACGTG ATATGCATTC CCCCTCCCGA GGCCCGGAAA 
GCAATCAACT GCATGTCCCT GAGCAAGTGC GAGGACCGCC TGGCCATCGC CTATGACAAC 
ATCGTCCTGG TGCTGGACAT CACCTCCGGG GACCCCTGCC CGGTCATCGA TGGGCCAAGA 
TACACCTTTT ACACTCAGCT GCCCGAGACC CTCTCCAGCG TGGCCATTCT GACGGACTAC 
CGCGTGGTCT ACAGCATGAC CAATGGGGAC CTCTTTCTTT ACGAGTGTGC AACTTCCAAA 
GCGTTTCCCT TGGAGACCCA CAGGAGCCGA GTTGCCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGTGG TCAGCGGGTC TGAGGATGCC CTGCTGTGTC TCTGGGACCT GCAGGCACGC 
AAGTGGAAAT TCGAGATGAG CTACACGAGT TCTTACTGCA GAGGGGTCCA ATGTGCTTGC 
TTCTCCAAGG ATGACAAGTA TGTGTACGTG GGCTTAAAGG ATCGCTCTAT ACTTGTTTGG 
AGTGTGCTGG ATGGCACCCT GCTGACAGTC CAGTTTGTCC ATGCTGTGGT AAACAGAATC 
ATCCCAACCA CCAGTGGCTT CATTGCCCCC ACCAGGCATG GTTATCTGAT CCGAGAGAAT 
TTCCAGTGCC TTTCAGCAAA AGCCTCACCG CAGGACCCTC TTAAGAACTT CAAGAAGGCA 
ATGTGGATGG TCAAATCCAG GCAGAGAGAG GAGCTAGTTG CAGCTGCAGG AGCTCCCCAG 
GACTTGGAGT CAGAAAGTGC CCAGGGAAAT GAAACCAAAT CAAACAAATG CTCACAAGTG 
TGCCTGATAG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_011527931.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526233.1 Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X3, mRNA.

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CloneID	OHu78810
Clone ID Related Accession (Same CDS sequence)	XM_011527929.1 , XM_011527931.2 , XM_011527930.2
Accession Version	XM_011527931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGTTTCCT CCAAAGGGGA CAGATTGCTG 
GAGAAGCTTC CAGATGCTGT GAGGTTCCTG GTGGTCTCTG AAGATGAGTC CCTCCTCGCC 
GCAGGCTTTG GAAGATCGGT GCGGATATTC TTGGCGGACT CGAGGGGCTT TCGCCGATTC 
ATGGCCATGG ATCTGGAACA TGAAGACATG GTGGAGACGG CTGTTTTTGG TACTGAGAAC 
AACCTGATCA TCACGGGGTC CCTTGATGCG CTCATTCAGG TGTGGAGTCT GTCAGAACAG 
GGGACCCTTC TGGACATCCT GGAAGGCGTC GGGGCCCCCG TGAGCCTGCT GGCCCGCGGC 
GGGGCTTTGG TGGCATCTGC TTCCCCACAG TCCTCATCTT TCAAGGTCTG GGATCTCAGC 
GATGCTCATA GGTCCCGGGT GCCTGCACCA TTTCTGGACC GCACCGGCCT CACCGCAGTG 
TCCCACAATG GAAGCTACGT CTACTTCCCC AAAATTGGGG ACAAAAACAA AGTCACTATT 
TGGGACTTGG CAGAAGGCGA GGAACAAGAT TCCCTGGACA CCTCCAGTGA GATCAGGTGT 
CTGGAGGTTG CTGAGCAGCG CAAGCTCCTA TTTACGGGCC TCGTGTCGGG GGTCGTCCTT 
GTGTTCCCCC TGAATTCCAG GCAGGACGTG ATATGCATTC CCCCTCCCGA GGCCCGGAAA 
GCAATCAACT GCATGTCCCT GAGCAAGTGC GAGGACCGCC TGGCCATCGC CTATGACAAC 
ATCGTCCTGG TGCTGGACAT CACCTCCGGG GACCCCTGCC CGGTCATCGA TGGGCCAAGA 
TACACCTTTT ACACTCAGCT GCCCGAGACC CTCTCCAGCG TGGCCATTCT GACGGACTAC 
CGCGTGGTCT ACAGCATGAC CAATGGGGAC CTCTTTCTTT ACGAGTGTGC AACTTCCAAA 
GCGTTTCCCT TGGAGACCCA CAGGAGCCGA GTTGCCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGTGG TCAGCGGGTC TGAGGATGCC CTGCTGTGTC TCTGGGACCT GCAGGCACGC 
AAGTGGAAAT TCGAGATGAG CTACACGAGT TCTTACTGCA GAGGGGTCCA ATGTGCTTGC 
TTCTCCAAGG ATGACAAGTA TGTGTACGTG GGCTTAAAGG ATCGCTCTAT ACTTGTTTGG 
AGTGTGCTGG ATGGCACCCT GCTGACAGTC CAGTTTGTCC ATGCTGTGGT AAACAGAATC 
ATCCCAACCA CCAGTGGCTT CATTGCCCCC ACCAGGCATG GTTATCTGAT CCGAGAGAAT 
TTCCAGTGCC TTTCAGCAAA AGCCTCACCG CAGGACCCTC TTAAGAACTT CAAGAAGGCA 
ATGTGGATGG TCAAATCCAG GCAGAGAGAG GAGCTAGTTG CAGCTGCAGG AGCTCCCCAG 
GACTTGGAGT CAGAAAGTGC CCAGGGAAAT GAAACCAAAT CAAACAAATG CTCACAAGTG 
TGCCTGATAG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526233.1
CDS	873..5567
Translation	MQRGKPCRALPTLKCQTFCQRHGLMFEVVDLRWGIRNIEATDHLTTELCLEEVDRCWKTSIGPAFVALIGDQYGPCLIPSRIDEKEWEVLRDHLTARPSDLELVARYFQRDENAFPPTYVLQAPGTGEACEPEEATLTSVLRSGAQEARRLGLITQEQWQHYHRSVIEWEIERSLLSSEDREQGATVFLREIQDLHKHILEDCALRMVDRLADGCLDADAQNLLSSLKSHITDMHPGVLKTHRLPWSRDLVNPKNKTHACYLKELGEQFVVRANHQVLTRLRELDTAGQELAWLYQEIRHHLWQSSEVIQTFCGRQELLARLGQQLRHDDSKQHTPLVLFGPPGIGKTALMCKLAEQMPRLLGHKTVTVLRLLGTSQMSSDARGLLKSICFQVCLAYGLPLPPAQVLDAHTRVVQFFHTLLHTVSCRNFESLVLLLDAMDDLDSVRHARRVPWLPLNCPPRVHLILSACSGALGVLDTLQRVLLDPEAYWEVKPLSGNQGQQMIQLLLAAARRTLSPVHTDLLWASLPECGNPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCTRLEQTHGQLLVAHVLGYIVSSRHGLSEAELKDVLSLDDEVLQDVYRDWTPPSKELLRFPPLLWVRLRRDLGYYLARRPVDGFTLLAIAHRQLVEVVRERYLSGSERAKRHGVLADFFSGTWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSHTVANLRKLKELPYHLLHSGRLEELKQEVLGSMSWISCRGISGGIEDLLDDFDLCAPHLDSPEVGLVREALQLCRPAVELRGMERSLLYTELLARLHFFATSHPALVGQLCQQAQSWFQLCAHPVLVPLGGFLQPPGGPLRATLSGCHKGITAMAWGVEEKLLVIGTQDGIMAVWDMEEQHVIHMLTGHTGEVRCVKIFAKGTLANSASKDYTLHLWNLLSGQEKFTIWDGGSKNPAEPQIWNLHVDEAHKVVYSASGSKINAWNLETAEPVFHILGDASDPWMCMAVLASQATLLTVSRDGVVSLWSSATGKLQGKQHMSSIKEETPTCAVSVQKQGKLVTGFSNGSISLVSSKGDRLLEKLPDAVRFLVVSEDESLLAAGFGRSVRIFLADSRGFRRFMAMDLEHEDMVETAVFGTENNLIITGSLDALIQVWSLSEQGTLLDILEGVGAPVSLLARGGALVASASPQSSSFKVWDLSDAHRSRVPAPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDLAEGEEQDSLDTSSEIRCLEVAEQRKLLFTGLVSGVVLVFPLNSRQDVICIPPPEARKAINCMSLSKCEDRLAIAYDNIVLVLDITSGDPCPVIDGPRYTFYTQLPETLSSVAILTDYRVVYSMTNGDLFLYECATSKAFPLETHRSRVACVEVSHKEQLVVSGSEDALLCLWDLQARKWKFEMSYTSSYCRGVQCACFSKDDKYVYVGLKDRSILVWSVLDGTLLTVQFVHAVVNRIIPTTSGFIAPTRHGYLIRENFQCLSAKASPQDPLKNFKKAMWMVKSRQREELVAAAGAPQDLESESAQGNETKSNKCSQVCLIV

Target ORF information:

RefSeq Version	XM_011527931.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527931.2

ORF Insert Sequence:

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGTTTCCT CCAAAGGGGA CAGATTGCTG 
GAGAAGCTTC CAGATGCTGT GAGGTTCCTG GTGGTCTCTG AAGATGAGTC CCTCCTCGCC 
GCAGGCTTTG GAAGATCGGT GCGGATATTC TTGGCGGACT CGAGGGGCTT TCGCCGATTC 
ATGGCCATGG ATCTGGAACA TGAAGACATG GTGGAGACGG CTGTTTTTGG TACTGAGAAC 
AACCTGATCA TCACGGGGTC CCTTGATGCG CTCATTCAGG TGTGGAGTCT GTCAGAACAG 
GGGACCCTTC TGGACATCCT GGAAGGCGTC GGGGCCCCCG TGAGCCTGCT GGCCCGCGGC 
GGGGCTTTGG TGGCATCTGC TTCCCCACAG TCCTCATCTT TCAAGGTCTG GGATCTCAGC 
GATGCTCATA GGTCCCGGGT GCCTGCACCA TTTCTGGACC GCACCGGCCT CACCGCAGTG 
TCCCACAATG GAAGCTACGT CTACTTCCCC AAAATTGGGG ACAAAAACAA AGTCACTATT 
TGGGACTTGG CAGAAGGCGA GGAACAAGAT TCCCTGGACA CCTCCAGTGA GATCAGGTGT 
CTGGAGGTTG CTGAGCAGCG CAAGCTCCTA TTTACGGGCC TCGTGTCGGG GGTCGTCCTT 
GTGTTCCCCC TGAATTCCAG GCAGGACGTG ATATGCATTC CCCCTCCCGA GGCCCGGAAA 
GCAATCAACT GCATGTCCCT GAGCAAGTGC GAGGACCGCC TGGCCATCGC CTATGACAAC 
ATCGTCCTGG TGCTGGACAT CACCTCCGGG GACCCCTGCC CGGTCATCGA TGGGCCAAGA 
TACACCTTTT ACACTCAGCT GCCCGAGACC CTCTCCAGCG TGGCCATTCT GACGGACTAC 
CGCGTGGTCT ACAGCATGAC CAATGGGGAC CTCTTTCTTT ACGAGTGTGC AACTTCCAAA 
GCGTTTCCCT TGGAGACCCA CAGGAGCCGA GTTGCCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGTGG TCAGCGGGTC TGAGGATGCC CTGCTGTGTC TCTGGGACCT GCAGGCACGC 
AAGTGGAAAT TCGAGATGAG CTACACGAGT TCTTACTGCA GAGGGGTCCA ATGTGCTTGC 
TTCTCCAAGG ATGACAAGTA TGTGTACGTG GGCTTAAAGG ATCGCTCTAT ACTTGTTTGG 
AGTGTGCTGG ATGGCACCCT GCTGACAGTC CAGTTTGTCC ATGCTGTGGT AAACAGAATC 
ATCCCAACCA CCAGTGGCTT CATTGCCCCC ACCAGGCATG GTTATCTGAT CCGAGAGAAT 
TTCCAGTGCC TTTCAGCAAA AGCCTCACCG CAGGACCCTC TTAAGAACTT CAAGAAGGCA 
ATGTGGATGG TCAAATCCAG GCAGAGAGAG GAGCTAGTTG CAGCTGCAGG AGCTCCCCAG 
GACTTGGAGT CAGAAAGTGC CCAGGGAAAT GAAACCAAAT CAAACAAATG CTCACAAGTG 
TGCCTGATAG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_011527930.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526232.1 Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu78810
Clone ID Related Accession (Same CDS sequence)	XM_011527929.1 , XM_011527931.2 , XM_011527930.2
Accession Version	XM_011527930.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527930.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGTTTCCT CCAAAGGGGA CAGATTGCTG 
GAGAAGCTTC CAGATGCTGT GAGGTTCCTG GTGGTCTCTG AAGATGAGTC CCTCCTCGCC 
GCAGGCTTTG GAAGATCGGT GCGGATATTC TTGGCGGACT CGAGGGGCTT TCGCCGATTC 
ATGGCCATGG ATCTGGAACA TGAAGACATG GTGGAGACGG CTGTTTTTGG TACTGAGAAC 
AACCTGATCA TCACGGGGTC CCTTGATGCG CTCATTCAGG TGTGGAGTCT GTCAGAACAG 
GGGACCCTTC TGGACATCCT GGAAGGCGTC GGGGCCCCCG TGAGCCTGCT GGCCCGCGGC 
GGGGCTTTGG TGGCATCTGC TTCCCCACAG TCCTCATCTT TCAAGGTCTG GGATCTCAGC 
GATGCTCATA GGTCCCGGGT GCCTGCACCA TTTCTGGACC GCACCGGCCT CACCGCAGTG 
TCCCACAATG GAAGCTACGT CTACTTCCCC AAAATTGGGG ACAAAAACAA AGTCACTATT 
TGGGACTTGG CAGAAGGCGA GGAACAAGAT TCCCTGGACA CCTCCAGTGA GATCAGGTGT 
CTGGAGGTTG CTGAGCAGCG CAAGCTCCTA TTTACGGGCC TCGTGTCGGG GGTCGTCCTT 
GTGTTCCCCC TGAATTCCAG GCAGGACGTG ATATGCATTC CCCCTCCCGA GGCCCGGAAA 
GCAATCAACT GCATGTCCCT GAGCAAGTGC GAGGACCGCC TGGCCATCGC CTATGACAAC 
ATCGTCCTGG TGCTGGACAT CACCTCCGGG GACCCCTGCC CGGTCATCGA TGGGCCAAGA 
TACACCTTTT ACACTCAGCT GCCCGAGACC CTCTCCAGCG TGGCCATTCT GACGGACTAC 
CGCGTGGTCT ACAGCATGAC CAATGGGGAC CTCTTTCTTT ACGAGTGTGC AACTTCCAAA 
GCGTTTCCCT TGGAGACCCA CAGGAGCCGA GTTGCCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGTGG TCAGCGGGTC TGAGGATGCC CTGCTGTGTC TCTGGGACCT GCAGGCACGC 
AAGTGGAAAT TCGAGATGAG CTACACGAGT TCTTACTGCA GAGGGGTCCA ATGTGCTTGC 
TTCTCCAAGG ATGACAAGTA TGTGTACGTG GGCTTAAAGG ATCGCTCTAT ACTTGTTTGG 
AGTGTGCTGG ATGGCACCCT GCTGACAGTC CAGTTTGTCC ATGCTGTGGT AAACAGAATC 
ATCCCAACCA CCAGTGGCTT CATTGCCCCC ACCAGGCATG GTTATCTGAT CCGAGAGAAT 
TTCCAGTGCC TTTCAGCAAA AGCCTCACCG CAGGACCCTC TTAAGAACTT CAAGAAGGCA 
ATGTGGATGG TCAAATCCAG GCAGAGAGAG GAGCTAGTTG CAGCTGCAGG AGCTCCCCAG 
GACTTGGAGT CAGAAAGTGC CCAGGGAAAT GAAACCAAAT CAAACAAATG CTCACAAGTG 
TGCCTGATAG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526232.1
CDS	1093..5787
Translation	MQRGKPCRALPTLKCQTFCQRHGLMFEVVDLRWGIRNIEATDHLTTELCLEEVDRCWKTSIGPAFVALIGDQYGPCLIPSRIDEKEWEVLRDHLTARPSDLELVARYFQRDENAFPPTYVLQAPGTGEACEPEEATLTSVLRSGAQEARRLGLITQEQWQHYHRSVIEWEIERSLLSSEDREQGATVFLREIQDLHKHILEDCALRMVDRLADGCLDADAQNLLSSLKSHITDMHPGVLKTHRLPWSRDLVNPKNKTHACYLKELGEQFVVRANHQVLTRLRELDTAGQELAWLYQEIRHHLWQSSEVIQTFCGRQELLARLGQQLRHDDSKQHTPLVLFGPPGIGKTALMCKLAEQMPRLLGHKTVTVLRLLGTSQMSSDARGLLKSICFQVCLAYGLPLPPAQVLDAHTRVVQFFHTLLHTVSCRNFESLVLLLDAMDDLDSVRHARRVPWLPLNCPPRVHLILSACSGALGVLDTLQRVLLDPEAYWEVKPLSGNQGQQMIQLLLAAARRTLSPVHTDLLWASLPECGNPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCTRLEQTHGQLLVAHVLGYIVSSRHGLSEAELKDVLSLDDEVLQDVYRDWTPPSKELLRFPPLLWVRLRRDLGYYLARRPVDGFTLLAIAHRQLVEVVRERYLSGSERAKRHGVLADFFSGTWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSHTVANLRKLKELPYHLLHSGRLEELKQEVLGSMSWISCRGISGGIEDLLDDFDLCAPHLDSPEVGLVREALQLCRPAVELRGMERSLLYTELLARLHFFATSHPALVGQLCQQAQSWFQLCAHPVLVPLGGFLQPPGGPLRATLSGCHKGITAMAWGVEEKLLVIGTQDGIMAVWDMEEQHVIHMLTGHTGEVRCVKIFAKGTLANSASKDYTLHLWNLLSGQEKFTIWDGGSKNPAEPQIWNLHVDEAHKVVYSASGSKINAWNLETAEPVFHILGDASDPWMCMAVLASQATLLTVSRDGVVSLWSSATGKLQGKQHMSSIKEETPTCAVSVQKQGKLVTGFSNGSISLVSSKGDRLLEKLPDAVRFLVVSEDESLLAAGFGRSVRIFLADSRGFRRFMAMDLEHEDMVETAVFGTENNLIITGSLDALIQVWSLSEQGTLLDILEGVGAPVSLLARGGALVASASPQSSSFKVWDLSDAHRSRVPAPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDLAEGEEQDSLDTSSEIRCLEVAEQRKLLFTGLVSGVVLVFPLNSRQDVICIPPPEARKAINCMSLSKCEDRLAIAYDNIVLVLDITSGDPCPVIDGPRYTFYTQLPETLSSVAILTDYRVVYSMTNGDLFLYECATSKAFPLETHRSRVACVEVSHKEQLVVSGSEDALLCLWDLQARKWKFEMSYTSSYCRGVQCACFSKDDKYVYVGLKDRSILVWSVLDGTLLTVQFVHAVVNRIIPTTSGFIAPTRHGYLIRENFQCLSAKASPQDPLKNFKKAMWMVKSRQREELVAAAGAPQDLESESAQGNETKSNKCSQVCLIV

Target ORF information:

RefSeq Version	XM_011527930.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527930.2

ORF Insert Sequence:

ATGCAGAGAG GGAAGCCCTG CAGAGCACTG CCTACCCTGA AGTGCCAGAC CTTCTGCCAG 
AGGCACGGCT TGATGTTTGA GGTCGTTGAT CTGAGGTGGG GTATTCGGAA CATTGAAGCC 
ACTGACCACT TGACCACAGA ACTCTGCTTG GAGGAGGTTG ACCGGTGTTG GAAAACATCC 
ATAGGGCCAG CTTTTGTTGC CCTCATCGGT GATCAGTACG GCCCCTGTCT GATTCCCTCG 
CGGATCGATG AGAAGGAGTG GGAGGTATTG AGGGACCATC TGACTGCCAG GCCAAGTGAC 
CTGGAGCTGG TGGCACGATA CTTCCAGAGG GACGAGAATG CGTTTCCTCC CACCTACGTC 
CTGCAGGCAC CAGGTACTGG GGAGGCCTGT GAACCAGAGG AGGCCACCTT AACTTCTGTC 
CTACGCTCTG GAGCCCAGGA GGCCCGGAGG CTGGGGCTCA TCACCCAGGA GCAGTGGCAG 
CACTACCACC GGTCAGTCAT TGAGTGGGAG ATAGAGCGGA GCCTGCTGAG CTCAGAGGAC 
CGGGAACAGG GAGCCACCGT CTTCCTTAGA GAGATCCAAG ACCTCCACAA ACACATCCTT 
GAAGACTGCG CCCTTAGGAT GGTGGACCGG CTCGCGGATG GCTGCCTGGA CGCTGATGCC 
CAGAACCTTC TCAGCAGCCT CAAAAGTCAC ATCACTGACA TGCACCCAGG GGTCCTCAAG 
ACCCACCGCC TGCCGTGGAG CCGCGACTTG GTGAACCCCA AGAACAAGAC TCACGCCTGC 
TACCTGAAGG AGCTGGGTGA GCAGTTTGTG GTGAGGGCCA ATCACCAGGT CCTCACACGC 
CTCCGTGAGC TGGATACGGC CGGACAGGAG TTGGCGTGGC TCTACCAAGA GATCCGCCAC 
CACCTTTGGC AGAGCTCGGA GGTCATTCAG ACCTTCTGCG GACGCCAGGA ACTCCTGGCC 
CGGCTTGGGC AGCAGCTCAG GCACGATGAC AGCAAGCAGC ACACCCCCCT GGTACTCTTT 
GGGCCCCCAG GCATTGGAAA GACAGCCCTG ATGTGCAAGC TGGCTGAGCA GATGCCAAGG 
CTGCTGGGGC ACAAGACAGT GACCGTCCTG CGGCTGCTGG GGACGTCACA AATGAGCTCA 
GATGCCCGTG GCCTGCTGAA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
TTGCCCCCTG CCCAGGTTCT GGACGCCCAC ACCAGGGTGG TCCAGTTTTT CCATACCCTC 
CTCCACACTG TCTCTTGCAG AAACTTCGAG TCTCTCGTGC TCCTGCTGGA TGCTATGGAT 
GACCTGGACT CTGTCCGCCA TGCTCGGAGG GTTCCCTGGC TGCCTCTCAA CTGCCCCCCG 
AGGGTGCACC TCATCCTCTC AGCTTGCTCG GGGGCACTGG GGGTTTTGGA CACCTTGCAG 
CGGGTGCTCC TGGACCCGGA GGCCTACTGG GAGGTGAAGC CCCTTTCCGG AAACCAAGGC 
CAGCAGATGA TCCAACTCCT GCTGGCAGCT GCAAGGAGGA CGCTGAGCCC GGTGCACACA 
GATTTGCTCT GGGCCAGCCT CCCAGAGTGT GGGAACCCAG GGCGGCTGAG GCTGGCGTTT 
GAGGAAGCCC GGAAATGGGC CTCTTTCACC GTGCCTGTCC CGCTGGCCAC CACCGCAGAG 
GAAGCCACGC ACCAACTCTG CACCCGCCTG GAGCAGACAC ACGGGCAGCT CCTCGTGGCC 
CACGTGCTGG GCTACATTGT GTCTTCCCGA CACGGTCTCT CGGAGGCGGA GCTGAAGGAT 
GTTTTGTCCC TGGACGACGA GGTCCTGCAG GATGTGTACC GAGATTGGAC CCCGCCCAGC 
AAGGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TTCGTCGGGA TCTGGGATAC 
TACTTGGCCC GGCGGCCCGT GGATGGCTTC ACCCTCCTGG CCATTGCCCA CAGACAGCTG 
GTCGAGGTGG TCCGTGAGCG CTACCTGTCA GGATCCGAGA GAGCCAAGAG GCATGGCGTC 
CTGGCCGACT TCTTCTCAGG GACCTGGAGC CAGGGTACCA AGAAGCTCAT CACTCTGCCA 
CTTGTGGGGA AACCACTGAA CTTGGACCGA AAGGTGGCCC CGCAGCCTCT GTGGTTCTCA 
CATACGGTTG CAAACCTGCG GAAGCTGAAG GAGTTGCCCT ATCACCTGCT TCACTCGGGC 
CGCCTGGAGG AGCTGAAACA GGAGGTTCTG GGCAGCATGA GCTGGATTTC CTGCCGGGGC 
ATCTCTGGGG GCATTGAAGA CCTGCTGGAT GACTTTGACC TGTGTGCCCC TCACCTGGAC 
TCCCCTGAGG TTGGCCTGGT CCGTGAAGCC CTCCAGCTCT GCCGCCCTGC TGTGGAGCTC 
CGAGGCATGG AGAGGAGCCT CCTGTACACA GAACTGCTGG CCAGACTCCA TTTCTTCGCC 
ACCTCACATC CAGCACTGGT GGGACAGCTA TGCCAACAGG CCCAGAGCTG GTTCCAGTTG 
TGCGCACACC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC AGCCCCCGGG AGGACCCCTC 
CGGGCAACTC TCAGCGGCTG TCACAAAGGC ATCACCGCCA TGGCATGGGG TGTGGAGGAG 
AAGCTGCTGG TGATTGGCAC CCAGGATGGC ATCATGGCTG TGTGGGACAT GGAAGAGCAG 
CATGTGATCC ACATGCTAAC TGGACACACA GGAGAGGTGA GGTGTGTGAA AATATTTGCC 
AAAGGGACCC TCGCCAACTC TGCTTCAAAG GATTACACGC TGCACTTGTG GAACTTACTC 
TCTGGCCAGG AGAAATTTAC CATTTGGGAT GGAGGCTCAA AAAATCCCGC TGAACCTCAG 
ATCTGGAACC TTCATGTGGA TGAGGCACAC AAAGTTGTGT ATTCAGCATC TGGCTCAAAG 
ATCAATGCTT GGAATCTGGA AACTGCAGAG CCGGTATTCC ATATCCTGGG AGATGCCTCT 
GATCCTTGGA TGTGCATGGC CGTGCTGGCC TCCCAGGCCA CACTGCTGAC AGTGTCCAGG 
GATGGTGTGG TCAGTCTGTG GAGCTCAGCT ACGGGAAAAC TTCAGGGGAA GCAACATATG 
TCCAGCATCA AAGAAGAAAC ACCTACCTGT GCCGTCTCAG TCCAGAAGCA AGGAAAGCTT 
GTTACCGGGT TTAGCAATGG CTCCATCTCT TTGGTTTCCT CCAAAGGGGA CAGATTGCTG 
GAGAAGCTTC CAGATGCTGT GAGGTTCCTG GTGGTCTCTG AAGATGAGTC CCTCCTCGCC 
GCAGGCTTTG GAAGATCGGT GCGGATATTC TTGGCGGACT CGAGGGGCTT TCGCCGATTC 
ATGGCCATGG ATCTGGAACA TGAAGACATG GTGGAGACGG CTGTTTTTGG TACTGAGAAC 
AACCTGATCA TCACGGGGTC CCTTGATGCG CTCATTCAGG TGTGGAGTCT GTCAGAACAG 
GGGACCCTTC TGGACATCCT GGAAGGCGTC GGGGCCCCCG TGAGCCTGCT GGCCCGCGGC 
GGGGCTTTGG TGGCATCTGC TTCCCCACAG TCCTCATCTT TCAAGGTCTG GGATCTCAGC 
GATGCTCATA GGTCCCGGGT GCCTGCACCA TTTCTGGACC GCACCGGCCT CACCGCAGTG 
TCCCACAATG GAAGCTACGT CTACTTCCCC AAAATTGGGG ACAAAAACAA AGTCACTATT 
TGGGACTTGG CAGAAGGCGA GGAACAAGAT TCCCTGGACA CCTCCAGTGA GATCAGGTGT 
CTGGAGGTTG CTGAGCAGCG CAAGCTCCTA TTTACGGGCC TCGTGTCGGG GGTCGTCCTT 
GTGTTCCCCC TGAATTCCAG GCAGGACGTG ATATGCATTC CCCCTCCCGA GGCCCGGAAA 
GCAATCAACT GCATGTCCCT GAGCAAGTGC GAGGACCGCC TGGCCATCGC CTATGACAAC 
ATCGTCCTGG TGCTGGACAT CACCTCCGGG GACCCCTGCC CGGTCATCGA TGGGCCAAGA 
TACACCTTTT ACACTCAGCT GCCCGAGACC CTCTCCAGCG TGGCCATTCT GACGGACTAC 
CGCGTGGTCT ACAGCATGAC CAATGGGGAC CTCTTTCTTT ACGAGTGTGC AACTTCCAAA 
GCGTTTCCCT TGGAGACCCA CAGGAGCCGA GTTGCCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGTGG TCAGCGGGTC TGAGGATGCC CTGCTGTGTC TCTGGGACCT GCAGGCACGC 
AAGTGGAAAT TCGAGATGAG CTACACGAGT TCTTACTGCA GAGGGGTCCA ATGTGCTTGC 
TTCTCCAAGG ATGACAAGTA TGTGTACGTG GGCTTAAAGG ATCGCTCTAT ACTTGTTTGG 
AGTGTGCTGG ATGGCACCCT GCTGACAGTC CAGTTTGTCC ATGCTGTGGT AAACAGAATC 
ATCCCAACCA CCAGTGGCTT CATTGCCCCC ACCAGGCATG GTTATCTGAT CCGAGAGAAT 
TTCCAGTGCC TTTCAGCAAA AGCCTCACCG CAGGACCCTC TTAAGAACTT CAAGAAGGCA 
ATGTGGATGG TCAAATCCAG GCAGAGAGAG GAGCTAGTTG CAGCTGCAGG AGCTCCCCAG 
GACTTGGAGT CAGAAAGTGC CCAGGGAAAT GAAACCAAAT CAAACAAATG CTCACAAGTG 
TGCCTGATAG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_017026669.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882158.1 Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu78811
Clone ID Related Accession (Same CDS sequence)	NM_001347994.1 , XM_017026669.1
Accession Version	XM_017026669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACC GGCTCGCGGA TGGCTGCCTG GACGCTGATG CCCAGAACCT TCTCAGCAGC 
CTCAAAAGTC ACATCACTGA CATGCACCCA GGGGTCCTCA AGACCCACCG CCTGCCGTGG 
AGCCGCGACT TGGTGAACCC CAAGAACAAG ACTCACGCCT GCTACCTGAA GGAGCTGGGT 
GAGCAGTTTG TGGTGAGGGC CAATCACCAG GTCCTCACAC GCCTCCGTGA GCTGGATACG 
GCCGGACAGG AGTTGGCGTG GCTCTACCAA GAGATCCGCC ACCACCTTTG GCAGAGCTCG 
GAGGTCATTC AGACCTTCTG CGGACGCCAG GAACTCCTGG CCCGGCTTGG GCAGCAGCTC 
AGGCACGATG ACAGCAAGCA GCACACCCCC CTGGTACTCT TTGGGCCCCC AGGCATTGGA 
AAGACAGCCC TGATGTGCAA GCTGGCTGAG CAGATGCCAA GGCTGCTGGG GCACAAGACA 
GTGACCGTCC TGCGGCTGCT GGGGACGTCA CAAATGAGCT CAGATGCCCG TGGCCTGCTG 
AAGAGCATCT GCTTCCAGGT GTGCCTGGCC TATGGGCTGC CCTTGCCCCC TGCCCAGGTT 
CTGGACGCCC ACACCAGGGT GGTCCAGTTT TTCCATACCC TCCTCCACAC TGTCTCTTGC 
AGAAACTTCG AGTCTCTCGT GCTCCTGCTG GATGCTATGG ATGACCTGGA CTCTGTCCGC 
CATGCTCGGA GGGTTCCCTG GCTGCCTCTC AACTGCCCCC CGAGGGTGCA CCTCATCCTC 
TCAGCTTGCT CGGGGGCACT GGGGGTTTTG GACACCTTGC AGCGGGTGCT CCTGGACCCG 
GAGGCCTACT GGGAGGTGAA GCCCCTTTCC GGAAACCAAG GCCAGCAGAT GATCCAACTC 
CTGCTGGCAG CTGCAAGGAG GACGCTGAGC CCGGTGCACA CAGATTTGCT CTGGGCCAGC 
CTCCCAGAGT GTGGGAACCC AGGGCGGCTG AGGCTGGCGT TTGAGGAAGC CCGGAAATGG 
GCCTCTTTCA CCGTGCCTGT CCCGCTGGCC ACCACCGCAG AGGAAGCCAC GCACCAACTC 
TGCACCCGCC TGGAGCAGAC ACACGGGCAG CTCCTCGTGG CCCACGTGCT GGGCTACATT 
GTGTCTTCCC GACACGGTCT CTCGGAGGCG GAGCTGAAGG ATGTTTTGTC CCTGGACGAC 
GAGGTCCTGC AGGATGTGTA CCGAGATTGG ACCCCGCCCA GCAAGGAGCT GCTGCGCTTC 
CCGCCCCTGC TGTGGGTGCG GCTTCGTCGG GATCTGGGAT ACTACTTGGC CCGGCGGCCC 
GTGGATGGCT TCACCCTCCT GGCCATTGCC CACAGACAGC TGGTCGAGGT GGTCCGTGAG 
CGCTACCTGT CAGGATCCGA GAGAGCCAAG AGGCATGGCG TCCTGGCCGA CTTCTTCTCA 
GGGACCTGGA GCCAGGGTAC CAAGAAGCTC ATCACTCTGC CACTTGTGGG GAAACCACTG 
AACTTGGACC GAAAGGTGGC CCCGCAGCCT CTGTGGTTCT CACATACGGT TGCAAACCTG 
CGGAAGCTGA AGGAGTTGCC CTATCACCTG CTTCACTCGG GCCGCCTGGA GGAGCTGAAA 
CAGGAGGTTC TGGGCAGCAT GAGCTGGATT TCCTGCCGGG GCATCTCTGG GGGCATTGAA 
GACCTGCTGG ATGACTTTGA CCTGTGTGCC CCTCACCTGG ACTCCCCTGA GGTTGGCCTG 
GTCCGTGAAG CCCTCCAGCT CTGCCGCCCT GCTGTGGAGC TCCGAGGCAT GGAGAGGAGC 
CTCCTGTACA CAGAACTGCT GGCCAGACTC CATTTCTTCG CCACCTCACA TCCAGCACTG 
GTGGGACAGC TATGCCAACA GGCCCAGAGC TGGTTCCAGT TGTGCGCACA CCCTGTGCTG 
GTGCCCCTCG GAGGATTCCT CCAGCCCCCG GGAGGACCCC TCCGGGCAAC TCTCAGCGGC 
TGTCACAAAG GCATCACCGC CATGGCATGG GGTGTGGAGG AGAAGCTGCT GGTGATTGGC 
ACCCAGGATG GCATCATGGC TGTGTGGGAC ATGGAAGAGC AGCATGTGAT CCACATGCTA 
ACTGGACACA CAGGAGAGGT GAGGTGTGTG AAAATATTTG CCAAAGGGAC CCTCGCCAAC 
TCTGCTTCAA AGGATTACAC GCTGCACTTG TGGAACTTAC TCTCTGGCCA GGAGAAATTT 
ACCATTTGGG ATGGAGGCTC AAAAAATCCC GCTGAACCTC AGATCTGGAA CCTTCATGTG 
GATGAGGCAC ACAAAGTTGT GTATTCAGCA TCTGGCTCAA AGATCAATGC TTGGAATCTG 
GAAACTGCAG AGCCGGTATT CCATATCCTG GGAGATGCCT CTGATCCTTG GATGTGCATG 
GCCGTGCTGG CCTCCCAGGC CACACTGCTG ACAGTGTCCA GGGATGGTGT GGTCAGTCTG 
TGGAGCTCAG CTACGGGAAA ACTTCAGGGG AAGCAACATA TGTCCAGCAT CAAAGAAGAA 
ACACCTACCT GTGCCGTCTC AGTCCAGAAG CAAGGAAAGC TTGTTACCGG GTTTAGCAAT 
GGCTCCATCT CTTTGGTTTC CTCCAAAGGG GACAGATTGC TGGAGAAGCT TCCAGATGCT 
GTGAGGTTCC TGGTGGTCTC TGAAGATGAG TCCCTCCTCG CCGCAGGCTT TGGAAGATCG 
GTGCGGATAT TCTTGGCGGA CTCGAGGGGC TTTCGCCGAT TCATGGCCAT GGATCTGGAA 
CATGAAGACA TGGTGGAGAC GGCTGTTTTT GGTACTGAGA ACAACCTGAT CATCACGGGG 
TCCCTTGATG CGCTCATTCA GGTGTGGAGT CTGTCAGAAC AGGGGACCCT TCTGGACATC 
CTGGAAGGCG TCGGGGCCCC CGTGAGCCTG CTGGCCCGCG GCGGGGCTTT GGTGGCATCT 
GCTTCCCCAC AGTCCTCATC TTTCAAGGTC TGGGATCTCA GCGATGCTCA TAGGTCCCGG 
GTGCCTGCAC CATTTCTGGA CCGCACCGGC CTCACCGCAG TGTCCCACAA TGGAAGCTAC 
GTCTACTTCC CCAAAATTGG GGACAAAAAC AAAGTCACTA TTTGGGACTT GGCAGAAGGC 
GAGGAACAAG ATTCCCTGGA CACCTCCAGT GAGATCAGGT GTCTGGAGGT TGCTGAGCAG 
CGCAAGCTCC TATTTACGGG CCTCGTGTCG GGGGTCGTCC TTGTGTTCCC CCTGAATTCC 
AGGCAGGACG TGATATGCAT TCCCCCTCCC GAGGCCCGGA AAGCAATCAA CTGCATGTCC 
CTGAGCAAGT GCGAGGACCG CCTGGCCATC GCCTATGACA ACATCGTCCT GGTGCTGGAC 
ATCACCTCCG GGGACCCCTG CCCGGTCATC GATGGGCCAA GATACACCTT TTACACTCAG 
CTGCCCGAGA CCCTCTCCAG CGTGGCCATT CTGACGGACT ACCGCGTGGT CTACAGCATG 
ACCAATGGGG ACCTCTTTCT TTACGAGTGT GCAACTTCCA AAGCGTTTCC CTTGGAGACC 
CACAGGAGCC GAGTTGCCTG TGTGGAGGTC AGCCACAAGG AGCAGCTGGT GGTCAGCGGG 
TCTGAGGATG CCCTGCTGTG TCTCTGGGAC CTGCAGGCAC GCAAGTGGAA ATTCGAGATG 
AGCTACACGA GTTCTTACTG CAGAGGGGTC CAATGTGCTT GCTTCTCCAA GGATGACAAG 
TATGTGTACG TGGGCTTAAA GGATCGCTCT ATACTTGTTT GGAGTGTGCT GGATGGCACC 
CTGCTGACAG TCCAGTTTGT CCATGCTGTG GTAAACAGAA TCATCCCAAC CACCAGTGGC 
TTCATTGCCC CCACCAGGCA TGGTTATCTG ATCCGAGAGA ATTTCCAGTG CCTTTCAGCA 
AAAGCCTCAC CGCAGGACCC TCTTAAGAAC TTCAAGAAGG CAATGTGGAT GGTCAAATCC 
AGGCAGAGAG AGGAGCTAGT TGCAGCTGCA GGAGCTCCCC AGGACTTGGA GTCAGAAAGT 
GCCCAGGGAA ATGAAACCAA ATCAAACAAA TGCTCACAAG TGTGCCTGAT AGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882158.1
CDS	1778..5854
Translation	MVDRLADGCLDADAQNLLSSLKSHITDMHPGVLKTHRLPWSRDLVNPKNKTHACYLKELGEQFVVRANHQVLTRLRELDTAGQELAWLYQEIRHHLWQSSEVIQTFCGRQELLARLGQQLRHDDSKQHTPLVLFGPPGIGKTALMCKLAEQMPRLLGHKTVTVLRLLGTSQMSSDARGLLKSICFQVCLAYGLPLPPAQVLDAHTRVVQFFHTLLHTVSCRNFESLVLLLDAMDDLDSVRHARRVPWLPLNCPPRVHLILSACSGALGVLDTLQRVLLDPEAYWEVKPLSGNQGQQMIQLLLAAARRTLSPVHTDLLWASLPECGNPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCTRLEQTHGQLLVAHVLGYIVSSRHGLSEAELKDVLSLDDEVLQDVYRDWTPPSKELLRFPPLLWVRLRRDLGYYLARRPVDGFTLLAIAHRQLVEVVRERYLSGSERAKRHGVLADFFSGTWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSHTVANLRKLKELPYHLLHSGRLEELKQEVLGSMSWISCRGISGGIEDLLDDFDLCAPHLDSPEVGLVREALQLCRPAVELRGMERSLLYTELLARLHFFATSHPALVGQLCQQAQSWFQLCAHPVLVPLGGFLQPPGGPLRATLSGCHKGITAMAWGVEEKLLVIGTQDGIMAVWDMEEQHVIHMLTGHTGEVRCVKIFAKGTLANSASKDYTLHLWNLLSGQEKFTIWDGGSKNPAEPQIWNLHVDEAHKVVYSASGSKINAWNLETAEPVFHILGDASDPWMCMAVLASQATLLTVSRDGVVSLWSSATGKLQGKQHMSSIKEETPTCAVSVQKQGKLVTGFSNGSISLVSSKGDRLLEKLPDAVRFLVVSEDESLLAAGFGRSVRIFLADSRGFRRFMAMDLEHEDMVETAVFGTENNLIITGSLDALIQVWSLSEQGTLLDILEGVGAPVSLLARGGALVASASPQSSSFKVWDLSDAHRSRVPAPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDLAEGEEQDSLDTSSEIRCLEVAEQRKLLFTGLVSGVVLVFPLNSRQDVICIPPPEARKAINCMSLSKCEDRLAIAYDNIVLVLDITSGDPCPVIDGPRYTFYTQLPETLSSVAILTDYRVVYSMTNGDLFLYECATSKAFPLETHRSRVACVEVSHKEQLVVSGSEDALLCLWDLQARKWKFEMSYTSSYCRGVQCACFSKDDKYVYVGLKDRSILVWSVLDGTLLTVQFVHAVVNRIIPTTSGFIAPTRHGYLIRENFQCLSAKASPQDPLKNFKKAMWMVKSRQREELVAAAGAPQDLESESAQGNETKSNKCSQVCLIV

Target ORF information:

RefSeq Version	XM_017026669.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NACHT and WD repeat domain containing 1 (NWD1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026669.1

ORF Insert Sequence:

ATGGTGGACC GGCTCGCGGA TGGCTGCCTG GACGCTGATG CCCAGAACCT TCTCAGCAGC 
CTCAAAAGTC ACATCACTGA CATGCACCCA GGGGTCCTCA AGACCCACCG CCTGCCGTGG 
AGCCGCGACT TGGTGAACCC CAAGAACAAG ACTCACGCCT GCTACCTGAA GGAGCTGGGT 
GAGCAGTTTG TGGTGAGGGC CAATCACCAG GTCCTCACAC GCCTCCGTGA GCTGGATACG 
GCCGGACAGG AGTTGGCGTG GCTCTACCAA GAGATCCGCC ACCACCTTTG GCAGAGCTCG 
GAGGTCATTC AGACCTTCTG CGGACGCCAG GAACTCCTGG CCCGGCTTGG GCAGCAGCTC 
AGGCACGATG ACAGCAAGCA GCACACCCCC CTGGTACTCT TTGGGCCCCC AGGCATTGGA 
AAGACAGCCC TGATGTGCAA GCTGGCTGAG CAGATGCCAA GGCTGCTGGG GCACAAGACA 
GTGACCGTCC TGCGGCTGCT GGGGACGTCA CAAATGAGCT CAGATGCCCG TGGCCTGCTG 
AAGAGCATCT GCTTCCAGGT GTGCCTGGCC TATGGGCTGC CCTTGCCCCC TGCCCAGGTT 
CTGGACGCCC ACACCAGGGT GGTCCAGTTT TTCCATACCC TCCTCCACAC TGTCTCTTGC 
AGAAACTTCG AGTCTCTCGT GCTCCTGCTG GATGCTATGG ATGACCTGGA CTCTGTCCGC 
CATGCTCGGA GGGTTCCCTG GCTGCCTCTC AACTGCCCCC CGAGGGTGCA CCTCATCCTC 
TCAGCTTGCT CGGGGGCACT GGGGGTTTTG GACACCTTGC AGCGGGTGCT CCTGGACCCG 
GAGGCCTACT GGGAGGTGAA GCCCCTTTCC GGAAACCAAG GCCAGCAGAT GATCCAACTC 
CTGCTGGCAG CTGCAAGGAG GACGCTGAGC CCGGTGCACA CAGATTTGCT CTGGGCCAGC 
CTCCCAGAGT GTGGGAACCC AGGGCGGCTG AGGCTGGCGT TTGAGGAAGC CCGGAAATGG 
GCCTCTTTCA CCGTGCCTGT CCCGCTGGCC ACCACCGCAG AGGAAGCCAC GCACCAACTC 
TGCACCCGCC TGGAGCAGAC ACACGGGCAG CTCCTCGTGG CCCACGTGCT GGGCTACATT 
GTGTCTTCCC GACACGGTCT CTCGGAGGCG GAGCTGAAGG ATGTTTTGTC CCTGGACGAC 
GAGGTCCTGC AGGATGTGTA CCGAGATTGG ACCCCGCCCA GCAAGGAGCT GCTGCGCTTC 
CCGCCCCTGC TGTGGGTGCG GCTTCGTCGG GATCTGGGAT ACTACTTGGC CCGGCGGCCC 
GTGGATGGCT TCACCCTCCT GGCCATTGCC CACAGACAGC TGGTCGAGGT GGTCCGTGAG 
CGCTACCTGT CAGGATCCGA GAGAGCCAAG AGGCATGGCG TCCTGGCCGA CTTCTTCTCA 
GGGACCTGGA GCCAGGGTAC CAAGAAGCTC ATCACTCTGC CACTTGTGGG GAAACCACTG 
AACTTGGACC GAAAGGTGGC CCCGCAGCCT CTGTGGTTCT CACATACGGT TGCAAACCTG 
CGGAAGCTGA AGGAGTTGCC CTATCACCTG CTTCACTCGG GCCGCCTGGA GGAGCTGAAA 
CAGGAGGTTC TGGGCAGCAT GAGCTGGATT TCCTGCCGGG GCATCTCTGG GGGCATTGAA 
GACCTGCTGG ATGACTTTGA CCTGTGTGCC CCTCACCTGG ACTCCCCTGA GGTTGGCCTG 
GTCCGTGAAG CCCTCCAGCT CTGCCGCCCT GCTGTGGAGC TCCGAGGCAT GGAGAGGAGC 
CTCCTGTACA CAGAACTGCT GGCCAGACTC CATTTCTTCG CCACCTCACA TCCAGCACTG 
GTGGGACAGC TATGCCAACA GGCCCAGAGC TGGTTCCAGT TGTGCGCACA CCCTGTGCTG 
GTGCCCCTCG GAGGATTCCT CCAGCCCCCG GGAGGACCCC TCCGGGCAAC TCTCAGCGGC 
TGTCACAAAG GCATCACCGC CATGGCATGG GGTGTGGAGG AGAAGCTGCT GGTGATTGGC 
ACCCAGGATG GCATCATGGC TGTGTGGGAC ATGGAAGAGC AGCATGTGAT CCACATGCTA 
ACTGGACACA CAGGAGAGGT GAGGTGTGTG AAAATATTTG CCAAAGGGAC CCTCGCCAAC 
TCTGCTTCAA AGGATTACAC GCTGCACTTG TGGAACTTAC TCTCTGGCCA GGAGAAATTT 
ACCATTTGGG ATGGAGGCTC AAAAAATCCC GCTGAACCTC AGATCTGGAA CCTTCATGTG 
GATGAGGCAC ACAAAGTTGT GTATTCAGCA TCTGGCTCAA AGATCAATGC TTGGAATCTG 
GAAACTGCAG AGCCGGTATT CCATATCCTG GGAGATGCCT CTGATCCTTG GATGTGCATG 
GCCGTGCTGG CCTCCCAGGC CACACTGCTG ACAGTGTCCA GGGATGGTGT GGTCAGTCTG 
TGGAGCTCAG CTACGGGAAA ACTTCAGGGG AAGCAACATA TGTCCAGCAT CAAAGAAGAA 
ACACCTACCT GTGCCGTCTC AGTCCAGAAG CAAGGAAAGC TTGTTACCGG GTTTAGCAAT 
GGCTCCATCT CTTTGGTTTC CTCCAAAGGG GACAGATTGC TGGAGAAGCT TCCAGATGCT 
GTGAGGTTCC TGGTGGTCTC TGAAGATGAG TCCCTCCTCG CCGCAGGCTT TGGAAGATCG 
GTGCGGATAT TCTTGGCGGA CTCGAGGGGC TTTCGCCGAT TCATGGCCAT GGATCTGGAA 
CATGAAGACA TGGTGGAGAC GGCTGTTTTT GGTACTGAGA ACAACCTGAT CATCACGGGG 
TCCCTTGATG CGCTCATTCA GGTGTGGAGT CTGTCAGAAC AGGGGACCCT TCTGGACATC 
CTGGAAGGCG TCGGGGCCCC CGTGAGCCTG CTGGCCCGCG GCGGGGCTTT GGTGGCATCT 
GCTTCCCCAC AGTCCTCATC TTTCAAGGTC TGGGATCTCA GCGATGCTCA TAGGTCCCGG 
GTGCCTGCAC CATTTCTGGA CCGCACCGGC CTCACCGCAG TGTCCCACAA TGGAAGCTAC 
GTCTACTTCC CCAAAATTGG GGACAAAAAC AAAGTCACTA TTTGGGACTT GGCAGAAGGC 
GAGGAACAAG ATTCCCTGGA CACCTCCAGT GAGATCAGGT GTCTGGAGGT TGCTGAGCAG 
CGCAAGCTCC TATTTACGGG CCTCGTGTCG GGGGTCGTCC TTGTGTTCCC CCTGAATTCC 
AGGCAGGACG TGATATGCAT TCCCCCTCCC GAGGCCCGGA AAGCAATCAA CTGCATGTCC 
CTGAGCAAGT GCGAGGACCG CCTGGCCATC GCCTATGACA ACATCGTCCT GGTGCTGGAC 
ATCACCTCCG GGGACCCCTG CCCGGTCATC GATGGGCCAA GATACACCTT TTACACTCAG 
CTGCCCGAGA CCCTCTCCAG CGTGGCCATT CTGACGGACT ACCGCGTGGT CTACAGCATG 
ACCAATGGGG ACCTCTTTCT TTACGAGTGT GCAACTTCCA AAGCGTTTCC CTTGGAGACC 
CACAGGAGCC GAGTTGCCTG TGTGGAGGTC AGCCACAAGG AGCAGCTGGT GGTCAGCGGG 
TCTGAGGATG CCCTGCTGTG TCTCTGGGAC CTGCAGGCAC GCAAGTGGAA ATTCGAGATG 
AGCTACACGA GTTCTTACTG CAGAGGGGTC CAATGTGCTT GCTTCTCCAA GGATGACAAG 
TATGTGTACG TGGGCTTAAA GGATCGCTCT ATACTTGTTT GGAGTGTGCT GGATGGCACC 
CTGCTGACAG TCCAGTTTGT CCATGCTGTG GTAAACAGAA TCATCCCAAC CACCAGTGGC 
TTCATTGCCC CCACCAGGCA TGGTTATCTG ATCCGAGAGA ATTTCCAGTG CCTTTCAGCA 
AAAGCCTCAC CGCAGGACCC TCTTAAGAAC TTCAAGAAGG CAATGTGGAT GGTCAAATCC 
AGGCAGAGAG AGGAGCTAGT TGCAGCTGCA GGAGCTCCCC AGGACTTGGA GTCAGAAAGT 
GCCCAGGGAA ATGAAACCAA ATCAAACAAA TGCTCACAAG TGTGCCTGAT AGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NWD1
Entrez Gene ID	284434
Full Name	NACHT and WD repeat domain containing 1
General protein information	Preferred Names NACHT and WD repeat domain containing 1 Names NACHT domain- and WD repeat-containing protein 1 NACHT and WD repeat domain-containing protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.11
Summary	The protein encoded by this gene is thought to be a cytosolic protein and predicted to contain a NACHT domain and multiple WD40 repeats. Increased expression of this gene was observed in some prostate cancer cell lines. Knocking down expression of this gene results in decreased androgen receptor protein levels, indicating that this gene may be important in modulating androgen receptor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016].
Disorder MIM:	616250

	LOC719640	XP_001114186.1
Canis lupus familiaris (dog)	NWD1	XP_533883.4
Xenopus tropicalis (tropical clawed frog)	nwd1	XP_002934424.2
Homo sapiens (human)	NWD1	NP_001007526.3
Pan troglodytes (chimpanzee)	NWD1	XP_003316227.2
Mus musculus (house mouse)	Nwd1	NP_795914.3
Bos taurus (cattle)	NWD1	NP_001095680.1
Rattus norvegicus (Norway rat)	LOC100364350	XP_006252881.1
Danio rerio (zebrafish)	LOC100330908	XP_002663751.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NWD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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