nwd1 cDNA ORF clone, Danio rerio(zebrafish)

The following nwd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nwd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa147942 XM_021479710.1
Latest version!
Danio rerio NACHT and WD repeat domain containing 1 (nwd1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa16761 XM_002663705.6
Latest version!
Danio rerio NACHT and WD repeat domain containing 1 (nwd1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa49402 XM_009305820.3
Latest version!
Danio rerio NACHT and WD repeat domain containing 1 (nwd1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
ODa16761 XM_002663705.5 Danio rerio NACHT and WD repeat domain containing 1 (nwd1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa49402 XM_009305820.2 Danio rerio NACHT and WD repeat domain containing 1 (nwd1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa147942
    Clone ID Related Accession (Same CDS sequence) XM_021479710.1
    Accession Version XM_021479710.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT domain- and WD repeat-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGTGACA TCACTGCATC TGATCATTTA ATAACTGAAC TGTGCATTAA AGAAATCGAA 
    ACCTGCCAGA GGATATCAGA TGGGCCAACT TTTATAGCTT TGATCGGTAA TCAGTATGGC
    CATCGCCTTG TCCCGCGCCT CATACCAGAG GCAGAGTTTG AGTTGCTGCT ATCAAAACTT
    TCCAAAGATC CAGAGAGTTT AAAGCTCCTT AACCAATGGT TTTACAAGGA CAGTAACTCA
    ATCCCACCCA CATATATCCT CCTGCCAATC ACGGTGCACT ACCCACATTA CAATGAGAGA
    GATTCTGAGA ACGCACAGTT GCGGGATAAT GATGTCATAT CCTGGAATCT AACAGAGTCA
    CGGCTGCTGC AGGTTCTCCG CAGTGCAGCT CTACAGGCTG AGAGAGACGG GGATTTCACT
    GCTGACAGCA AGCACAAGTT TTTCAAATCT GTTTTAGAAT ATGAGATGGA GCATGGCTTT
    CTCAGGACTC AAAGGGATGC ATCGGCTGTG CTGTTTTTCC GCGAACTGCC TCGACTTAGT
    AAAAGAGACT GCAAGAAAAA CTTTGCAAAG TTTCTGCTGG ACGTCACAGC CGACGGCTTG
    CTGGACACAC AAGCCCGGGA GCTCTTGACT GACCTGAAGC AACGCATCTG CAACACCGGT
    CGCCTCCAAC CACTCTGTAT GGAGCTCAGC AAAGGAGCCA TGGACCCAAG CCGCAAAGAA
    CACAAGGAAT ACCTGGGTAA GCTGTGTGAA CAGGTAACAT CTCAATTGAA GGAACTGATC
    AGCAGAAATG CTCTGCAAAC CTCAGGAACT TCAGTAGATC CAGTATGGTC CTGGCTAAAA
    CAGGAGATCT TTCATCATGC CAAGTTGAGT GAGGAGAAGT GTGCTGTGTT TAAAGGGAGA
    GACGACATTC TGGGAAAGAT CTCCATCACC CTGTGGGAAT CCAATGATGT TTCTCATGGA
    CCTCTTCTAG TCCATGGACC TCCAGGAGTT GGTAAAACAG CTCTGCTTTG CAAACTGGCC
    AAGGAAATGC GAGCAGTTGT TGACAATCGG GGAGTTGTTC TGCTGAGGTT GCTGGGAACA
    TCTCTGCTCA GTTCAGATGT CGATTCTGTT CTTCGAGGTG TTTGTCTTCA AGTGTGTGGA
    GCTCTAGGGT TGCAGTCCCC ATACTCGCAT ATAGCGTACA CGCATGAAGG GCTAGTTCGA
    TTTTTCCATT CCATGCTGGA AGACGTGTCA AAACAAGGAG ACACTCTTCT ACTCATTCTA
    GACTCATTGG AGCAGCTTTT AGAGACAAAC AAACTTCATA AACTAGACTG GATACCTAAA
    ATAATTCCAC CCAATGTGCA AATCGTATTC TCAACTTCTA ATGAGTGCTT GGACAAATGT
    AAATCTTTGG TTTCAGCGGA AGAAAACATC TTTAAATTGG AACTTCTCAC GAAAGATCAA
    GGGAGAGACG TCATTGATGC ATATATGAGT GCAGCTGAAC GCAAGCTGAC GGCAGAACAG
    CAAGACGCAA TCTTACGCTA CTTTGAGCAA AGTGGAAACC CTTTGCATCT GAAACTCATG
    CTAGACACTG CCAAACTGTG GTCCTCCTAC ACCCCCATGT CTAGTGTTCA CCTGGACAAG
    TCGGCGGAGG AAGTGATGAC ACAGTTTGTC GAGTCACTTG AGGTGAATCA TGGGAAGAAG
    CTAGTCAGTC ATGCTCTGAG CTTCATCCTG TTATCAAGAT ATGGACTTTC AGAGGCAGAG
    TTACAGGATG TCCTATCATT AGATAATGAT GTTCTAGCTG AGATCTATAA ATGCTGGCTT
    CCTCCGAGCC ATACGCTCAT GCGCTTCCCA CGTTTTCATT GGTCAAGACT CAGGCATGAC
    CTGAGTGCTC ATCTGACGGA GAGATGGGAA GCCGGCGTTC TTCTGCTGGG TTTCACTCAC
    AGGCAGTTTG CAGAACTGGT AAGGAGGAGA TATTTGTCAC GTGATGTGAA AGCTGACATG
    CACACGATCC TCGCCGAGTA TTTCTCCGGA CAGTGGAGTA AGGGGCAGCT CAGACCCATC
    CTTCTCCCCT CACTGAGCGC ATTTCTCAAT GCTGACAGAA AGGTATCACC CCAGCCCTTG
    TGGTTTTCTG AAGAACTTGC CAATTTGCGT AAGATTCATG AACTTCCTTA TCACCTTGCT
    CACGCAGGAA AATGGGACGA GCTACGACAA TCAGTGCTTG GGAGTCTGGA CTGGTTGTGC
    TGTAAGACTC TGCAGTGTGG CGTGAGCTGT GTTATTCAGG ACCTGACCCT TTGCACTGAC
    CTCACTGACT GCCCTGAGAT ACAGTTGCTG AAGGAGACGT TTTTGCTTCT TAAACCCTCG
    CTGGATTTTA GCGACGGCCG CGTGGATCCC TCGCTGTTAG TGACAGAGAT CTTTGCCAGG
    CTACAGGGTC TTGCTGATCA TTACCCCTCC ATGATTGGTC AACTGTGTTC TCAATGTATG
    GACTGGTTTG AATCCTATTC TGACCCATTA CTTGTGCCCA AATGCAGCTT CTTTGAAGCC
    CCTGGTGGCC CACTAAAGAG CACCCTCAGA GGATTCACTA AAGGGGTGAA GGCTGTTGTT
    CTGTGCTCAA AGAGGAAGCT GCTGTTAGCA GGTTCAGAGG ATGGACTGAT CATAGTCTGG
    AGTCTAAACA ATCTGCAGCC TCTTCACACT CTTTCTGGAC ATAAAGCCGG GGTGCTGTCC
    CTGAAGCTGA CGGACAAAAC CAATAACTGT TTGTCTACTG GGCTCGATGG GACTCTGAGA
    AAATGGTGTC TGATAAGTGG ACTGCAGCTT TTCTGCATCA CTGATGCCGT TTCTGTCCAA
    ACACACAGCC CTACACACAT CCATATGAAT GAAGAAAGGA AGATCATCAT AACCAACCCA
    AGCAGCCAAG TTAAAGCTTG GGATTTGGAC ACTGCAGAGC CTCTGTTTGA GCTGACCGCA
    GTGATTGGTT CTGTTCTTGG CATTGTGGAA GATGAAATAG CGTGCATATG CAGTGAGGCG
    ATACTCTGCT TTTATGACAT ATCCACCGGC ACTCAGACTT CCCAGACCCT CCTGCCCTAC
    TCTGCCGGAC ACCATAACAT CACCTGCTGT CTCACACTCA CCAAACACTC CAAGATACTC
    ATTGCTTTTG GGGAAAGCCG TCTACATTCG GTGTGGAGCC ATGAAATGGA GTCTGTGATT
    GATCTTCCAT CTCCAGCTCT CTTTCTCAGA ACATCTGAGG ATGAAAAACT GCTGCTTGCA
    GGATGTGACA GAACATTGTG CGTGTTCCTC GTGACATTGA CTTCAATGCA CAAGGTTCTG
    GATCTTCAAC ATGATGCATC TATCCTGTGC GCCGTGTCAA ACAGCGAAGG TAGCGAAATC
    ATCACGAGTG CCCAGGATCG TATAATACGG GTATGGAGCG TAACCACAGG AGCCCTGCTG
    GACTGGATCG GTGTGATGGA TTCCTCTGTG TCCTCATTGG CTGTACATCA GCGCTTTATT
    ATTTCCGCCT CTGTTATTGT CAACCAGCTT AAAGTATGGC AGCTTGACCA CAACACCACG
    CACAGGAACA AGAGTTTCAT CCCCGCATAC TGCCCCTTGG CAGTCCTGTC GAAAGATGGT
    GATACTGTAT TCTATGTCAA GGAGGGAAAT AAAACCGAGG TTTTCACCTG GAGCTGTTCG
    GAAGGTGAAA CATGTCTGCA GTTAGACAGC ATGGACGTTT CCTCAGAGGT GTGCTGTATG
    GAATTAGCCC AGCAGAAACG GCTCCTCTTC TGCGGTCTGC ACACTGGAAC CATCTTTATT
    TACCCGCTTG ACTTTATCCC AGAAACCTTG TGCCTACCGC CTCCGGAAAG CCTACCAATG
    GTACGCAGCA TGGCCATCAG CCCACGAGAG GACAAAATGG CCGTCGCATA TGAGGATGCC
    GTCTGTCTGT TTGAGATTAC AGCGAGGGAC AGCTTCCCTT GCGTCGATGG ACCATTTGAA
    AGGTACTCGC TTGACCTGTT ACATTCCTCA ATCTCCGCCA TGGCTTTGTT GTCCGACTGC
    AGGCTGCTGT ATGGGACGTT TGGCGGCGAA GTGGTGATCT ACGACTTTAA AAGCGCCACA
    GCAGCAGAGC TGGATCACCA CGGTGCAGCC ATAACCTGCA TCACCATGAG CAACTGGGAT
    GCGCAGGCAC TGATTGGCTC TCAGGACTGT GTTCAGAAGC TGTGGAATTT GAGTCCCGTC
    TTACTGAATC ACACCATGGA GTACAAGGGC TTTTATTTTG AAGGAGTTCT ATGTGCTGTC
    TTCTCAATAA ATGATAAATA CGTGTTCACT GGATCCTTGG ACAAAACCGT CAAAGTCTGG
    GATGTTTCGA GTGGTTGTTT GCTGTATATC CAGTACGTCT ACTCTCCGGT CATAAGGATG
    ATGCCACACA AGGATGGATT TGTAGCTGTA TCCCAGCATG GCTCATTCAT CAAGGAAGGT
    TTTCGCTGTC CAGACAGCCT CAAGCCTGGA TATAACCCCC TCCGGAACTT CCAAGTACAT
    TACAAAGTCA CTTCACGAGA CAAAAGTCTG AACGTTCCAC ACAGCGTGAT CAATGAGCTG
    CCAGATTACA ACCCTGCTCA GTTCAACTTC ATTGGCATCG GTCTGATCAA ATCCAAACCA
    TCCAATACGT GCGTTCTCCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335385.1
    CDS102..4745
    Translation

    Target ORF information:

    RefSeq Version XM_021479710.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT and WD repeat domain containing 1 (nwd1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479710.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGTGACA TCACTGCATC TGATCATTTA ATAACTGAAC TGTGCATTAA AGAAATCGAA 
    ACCTGCCAGA GGATATCAGA TGGGCCAACT TTTATAGCTT TGATCGGTAA TCAGTATGGC
    CATCGCCTTG TCCCGCGCCT CATACCAGAG GCAGAGTTTG AGTTGCTGCT ATCAAAACTT
    TCCAAAGATC CAGAGAGTTT AAAGCTCCTT AACCAATGGT TTTACAAGGA CAGTAACTCA
    ATCCCACCCA CATATATCCT CCTGCCAATC ACGGTGCACT ACCCACATTA CAATGAGAGA
    GATTCTGAGA ACGCACAGTT GCGGGATAAT GATGTCATAT CCTGGAATCT AACAGAGTCA
    CGGCTGCTGC AGGTTCTCCG CAGTGCAGCT CTACAGGCTG AGAGAGACGG GGATTTCACT
    GCTGACAGCA AGCACAAGTT TTTCAAATCT GTTTTAGAAT ATGAGATGGA GCATGGCTTT
    CTCAGGACTC AAAGGGATGC ATCGGCTGTG CTGTTTTTCC GCGAACTGCC TCGACTTAGT
    AAAAGAGACT GCAAGAAAAA CTTTGCAAAG TTTCTGCTGG ACGTCACAGC CGACGGCTTG
    CTGGACACAC AAGCCCGGGA GCTCTTGACT GACCTGAAGC AACGCATCTG CAACACCGGT
    CGCCTCCAAC CACTCTGTAT GGAGCTCAGC AAAGGAGCCA TGGACCCAAG CCGCAAAGAA
    CACAAGGAAT ACCTGGGTAA GCTGTGTGAA CAGGTAACAT CTCAATTGAA GGAACTGATC
    AGCAGAAATG CTCTGCAAAC CTCAGGAACT TCAGTAGATC CAGTATGGTC CTGGCTAAAA
    CAGGAGATCT TTCATCATGC CAAGTTGAGT GAGGAGAAGT GTGCTGTGTT TAAAGGGAGA
    GACGACATTC TGGGAAAGAT CTCCATCACC CTGTGGGAAT CCAATGATGT TTCTCATGGA
    CCTCTTCTAG TCCATGGACC TCCAGGAGTT GGTAAAACAG CTCTGCTTTG CAAACTGGCC
    AAGGAAATGC GAGCAGTTGT TGACAATCGG GGAGTTGTTC TGCTGAGGTT GCTGGGAACA
    TCTCTGCTCA GTTCAGATGT CGATTCTGTT CTTCGAGGTG TTTGTCTTCA AGTGTGTGGA
    GCTCTAGGGT TGCAGTCCCC ATACTCGCAT ATAGCGTACA CGCATGAAGG GCTAGTTCGA
    TTTTTCCATT CCATGCTGGA AGACGTGTCA AAACAAGGAG ACACTCTTCT ACTCATTCTA
    GACTCATTGG AGCAGCTTTT AGAGACAAAC AAACTTCATA AACTAGACTG GATACCTAAA
    ATAATTCCAC CCAATGTGCA AATCGTATTC TCAACTTCTA ATGAGTGCTT GGACAAATGT
    AAATCTTTGG TTTCAGCGGA AGAAAACATC TTTAAATTGG AACTTCTCAC GAAAGATCAA
    GGGAGAGACG TCATTGATGC ATATATGAGT GCAGCTGAAC GCAAGCTGAC GGCAGAACAG
    CAAGACGCAA TCTTACGCTA CTTTGAGCAA AGTGGAAACC CTTTGCATCT GAAACTCATG
    CTAGACACTG CCAAACTGTG GTCCTCCTAC ACCCCCATGT CTAGTGTTCA CCTGGACAAG
    TCGGCGGAGG AAGTGATGAC ACAGTTTGTC GAGTCACTTG AGGTGAATCA TGGGAAGAAG
    CTAGTCAGTC ATGCTCTGAG CTTCATCCTG TTATCAAGAT ATGGACTTTC AGAGGCAGAG
    TTACAGGATG TCCTATCATT AGATAATGAT GTTCTAGCTG AGATCTATAA ATGCTGGCTT
    CCTCCGAGCC ATACGCTCAT GCGCTTCCCA CGTTTTCATT GGTCAAGACT CAGGCATGAC
    CTGAGTGCTC ATCTGACGGA GAGATGGGAA GCCGGCGTTC TTCTGCTGGG TTTCACTCAC
    AGGCAGTTTG CAGAACTGGT AAGGAGGAGA TATTTGTCAC GTGATGTGAA AGCTGACATG
    CACACGATCC TCGCCGAGTA TTTCTCCGGA CAGTGGAGTA AGGGGCAGCT CAGACCCATC
    CTTCTCCCCT CACTGAGCGC ATTTCTCAAT GCTGACAGAA AGGTATCACC CCAGCCCTTG
    TGGTTTTCTG AAGAACTTGC CAATTTGCGT AAGATTCATG AACTTCCTTA TCACCTTGCT
    CACGCAGGAA AATGGGACGA GCTACGACAA TCAGTGCTTG GGAGTCTGGA CTGGTTGTGC
    TGTAAGACTC TGCAGTGTGG CGTGAGCTGT GTTATTCAGG ACCTGACCCT TTGCACTGAC
    CTCACTGACT GCCCTGAGAT ACAGTTGCTG AAGGAGACGT TTTTGCTTCT TAAACCCTCG
    CTGGATTTTA GCGACGGCCG CGTGGATCCC TCGCTGTTAG TGACAGAGAT CTTTGCCAGG
    CTACAGGGTC TTGCTGATCA TTACCCCTCC ATGATTGGTC AACTGTGTTC TCAATGTATG
    GACTGGTTTG AATCCTATTC TGACCCATTA CTTGTGCCCA AATGCAGCTT CTTTGAAGCC
    CCTGGTGGCC CACTAAAGAG CACCCTCAGA GGATTCACTA AAGGGGTGAA GGCTGTTGTT
    CTGTGCTCAA AGAGGAAGCT GCTGTTAGCA GGTTCAGAGG ATGGACTGAT CATAGTCTGG
    AGTCTAAACA ATCTGCAGCC TCTTCACACT CTTTCTGGAC ATAAAGCCGG GGTGCTGTCC
    CTGAAGCTGA CGGACAAAAC CAATAACTGT TTGTCTACTG GGCTCGATGG GACTCTGAGA
    AAATGGTGTC TGATAAGTGG ACTGCAGCTT TTCTGCATCA CTGATGCCGT TTCTGTCCAA
    ACACACAGCC CTACACACAT CCATATGAAT GAAGAAAGGA AGATCATCAT AACCAACCCA
    AGCAGCCAAG TTAAAGCTTG GGATTTGGAC ACTGCAGAGC CTCTGTTTGA GCTGACCGCA
    GTGATTGGTT CTGTTCTTGG CATTGTGGAA GATGAAATAG CGTGCATATG CAGTGAGGCG
    ATACTCTGCT TTTATGACAT ATCCACCGGC ACTCAGACTT CCCAGACCCT CCTGCCCTAC
    TCTGCCGGAC ACCATAACAT CACCTGCTGT CTCACACTCA CCAAACACTC CAAGATACTC
    ATTGCTTTTG GGGAAAGCCG TCTACATTCG GTGTGGAGCC ATGAAATGGA GTCTGTGATT
    GATCTTCCAT CTCCAGCTCT CTTTCTCAGA ACATCTGAGG ATGAAAAACT GCTGCTTGCA
    GGATGTGACA GAACATTGTG CGTGTTCCTC GTGACATTGA CTTCAATGCA CAAGGTTCTG
    GATCTTCAAC ATGATGCATC TATCCTGTGC GCCGTGTCAA ACAGCGAAGG TAGCGAAATC
    ATCACGAGTG CCCAGGATCG TATAATACGG GTATGGAGCG TAACCACAGG AGCCCTGCTG
    GACTGGATCG GTGTGATGGA TTCCTCTGTG TCCTCATTGG CTGTACATCA GCGCTTTATT
    ATTTCCGCCT CTGTTATTGT CAACCAGCTT AAAGTATGGC AGCTTGACCA CAACACCACG
    CACAGGAACA AGAGTTTCAT CCCCGCATAC TGCCCCTTGG CAGTCCTGTC GAAAGATGGT
    GATACTGTAT TCTATGTCAA GGAGGGAAAT AAAACCGAGG TTTTCACCTG GAGCTGTTCG
    GAAGGTGAAA CATGTCTGCA GTTAGACAGC ATGGACGTTT CCTCAGAGGT GTGCTGTATG
    GAATTAGCCC AGCAGAAACG GCTCCTCTTC TGCGGTCTGC ACACTGGAAC CATCTTTATT
    TACCCGCTTG ACTTTATCCC AGAAACCTTG TGCCTACCGC CTCCGGAAAG CCTACCAATG
    GTACGCAGCA TGGCCATCAG CCCACGAGAG GACAAAATGG CCGTCGCATA TGAGGATGCC
    GTCTGTCTGT TTGAGATTAC AGCGAGGGAC AGCTTCCCTT GCGTCGATGG ACCATTTGAA
    AGGTACTCGC TTGACCTGTT ACATTCCTCA ATCTCCGCCA TGGCTTTGTT GTCCGACTGC
    AGGCTGCTGT ATGGGACGTT TGGCGGCGAA GTGGTGATCT ACGACTTTAA AAGCGCCACA
    GCAGCAGAGC TGGATCACCA CGGTGCAGCC ATAACCTGCA TCACCATGAG CAACTGGGAT
    GCGCAGGCAC TGATTGGCTC TCAGGACTGT GTTCAGAAGC TGTGGAATTT GAGTCCCGTC
    TTACTGAATC ACACCATGGA GTACAAGGGC TTTTATTTTG AAGGAGTTCT ATGTGCTGTC
    TTCTCAATAA ATGATAAATA CGTGTTCACT GGATCCTTGG ACAAAACCGT CAAAGTCTGG
    GATGTTTCGA GTGGTTGTTT GCTGTATATC CAGTACGTCT ACTCTCCGGT CATAAGGATG
    ATGCCACACA AGGATGGATT TGTAGCTGTA TCCCAGCATG GCTCATTCAT CAAGGAAGGT
    TTTCGCTGTC CAGACAGCCT CAAGCCTGGA TATAACCCCC TCCGGAACTT CCAAGTACAT
    TACAAAGTCA CTTCACGAGA CAAAAGTCTG AACGTTCCAC ACAGCGTGAT CAATGAGCTG
    CCAGATTACA ACCCTGCTCA GTTCAACTTC ATTGGCATCG GTCTGATCAA ATCCAAACCA
    TCCAATACGT GCGTTCTCCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa16761
    Clone ID Related Accession (Same CDS sequence) XM_002663705.6 , XM_002663705.5
    Accession Version XM_002663705.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT domain- and WD repeat-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002663705.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGATGTGG TGGAGATGAG AGAGGCACTT GGGTTGGAGG TTCTGCGGGG ACAAAGCGCT 
    GATTCTCTGC AGCCTACTTC CAGAATGGTG CGGGTGTTCA TCAGCTCCAC TCACTCAGAC
    ATGAAAAATG AGAGAAATGC ATTCTGGGAG AAGACCTATT CGGAGCTACA GACCCACTGC
    CAAAGTTTGG GTCTTGTTTT TGAGGTAGTG GATTTCAAAT GTGGAATGCG TGACATCACT
    GCATCTGATC ATTTAATAAC TGAACTGTGC ATTAAAGAAA TCGAAACCTG CCAGAGGATA
    TCAGATGGGC CAACTTTTAT AGCTTTGATC GGTAATCAGT ATGGCCATCG CCTTGTCCCG
    CGCCTCATAC CAGAGGCAGA GTTTGAGTTG CTGCTATCAA AACTTTCCAA AGATCCAGAG
    AGTTTAAAGC TCCTTAACCA ATGGTTTTAC AAGGACAGTA ACTCAATCCC ACCCACATAT
    ATCCTCCTGC CAATCACGGT GCACTACCCA CATTACAATG AGAGAGATTC TGAGAACGCA
    CAGTTGCGGG ATAATGATGT CATATCCTGG AATCTAACAG AGTCACGGCT GCTGCAGGTT
    CTCCGCAGTG CAGCTCTACA GGCTGAGAGA GACGGGGATT TCACTGCTGA CAGCAAGCAC
    AAGTTTTTCA AATCTGTTTT AGAATATGAG ATGGAGCATG GCTTTCTCAG GACTCAAAGG
    GATGCATCGG CTGTGCTGTT TTTCCGCGAA CTGCCTCGAC TTAGTAAAAG AGACTGCAAG
    AAAAACTTTG CAAAGTTTCT GCTGGACGTC ACAGCCGACG GCTTGCTGGA CACACAAGCC
    CGGGAGCTCT TGACTGACCT GAAGCAACGC ATCTGCAACA CCGGTCGCCT CCAACCACTC
    TGTATGGAGC TCAGCAAAGG AGCCATGGAC CCAAGCCGCA AAGAACACAA GGAATACCTG
    GGTAAGCTGT GTGAACAGGT AACATCTCAA TTGAAGGAAC TGATCAGCAG AAATGCTCTG
    CAAACCTCAG GAACTTCAGT AGATCCAGTA TGGTCCTGGC TAAAACAGGA GATCTTTCAT
    CATGCCAAGT TGAGTGAGGA GAAGTGTGCT GTGTTTAAAG GGAGAGACGA CATTCTGGGA
    AAGATCTCCA TCACCCTGTG GGAATCCAAT GATGTTTCTC ATGGACCTCT TCTAGTCCAT
    GGACCTCCAG GAGTTGGTAA AACAGCTCTG CTTTGCAAAC TGGCCAAGGA AATGCGAGCA
    GTTGTTGACA ATCGGGGAGT TGTTCTGCTG AGGTTGCTGG GAACATCTCT GCTCAGTTCA
    GATGTCGATT CTGTTCTTCG AGGTGTTTGT CTTCAAGTGT GTGGAGCTCT AGGGTTGCAG
    TCCCCATACT CGCATATAGC GTACACGCAT GAAGGGCTAG TTCGATTTTT CCATTCCATG
    CTGGAAGACG TGTCAAAACA AGGAGACACT CTTCTACTCA TTCTAGACTC ATTGGAGCAG
    CTTTTAGAGA CAAACAAACT TCATAAACTA GACTGGATAC CTAAAATAAT TCCACCCAAT
    GTGCAAATCG TATTCTCAAC TTCTAATGAG TGCTTGGACA AATGTAAATC TTTGGTTTCA
    GCGGAAGAAA ACATCTTTAA ATTGGAACTT CTCACGAAAG ATCAAGGGAG AGACGTCATT
    GATGCATATA TGAGTGCAGC TGAACGCAAG CTGACGGCAG AACAGCAAGA CGCAATCTTA
    CGCTACTTTG AGCAAAGTGG AAACCCTTTG CATCTGAAAC TCATGCTAGA CACTGCCAAA
    CTGTGGTCCT CCTACACCCC CATGTCTAGT GTTCACCTGG ACAAGTCGGC GGAGGAAGTG
    ATGACACAGT TTGTCGAGTC ACTTGAGGTG AATCATGGGA AGAAGCTAGT CAGTCATGCT
    CTGAGCTTCA TCCTGTTATC AAGATATGGA CTTTCAGAGG CAGAGTTACA GGATGTCCTA
    TCATTAGATA ATGATGTTCT AGCTGAGATC TATAAATGCT GGCTTCCTCC GAGCCATACG
    CTCATGCGCT TCCCACGTTT TCATTGGTCA AGACTCAGGC ATGACCTGAG TGCTCATCTG
    ACGGAGAGAT GGGAAGCCGG CGTTCTTCTG CTGGGTTTCA CTCACAGGCA GTTTGCAGAA
    CTGGTAAGGA GGAGATATTT GTCACGTGAT GTGAAAGCTG ACATGCACAC GATCCTCGCC
    GAGTATTTCT CCGGACAGTG GAGTAAGGGG CAGCTCAGAC CCATCCTTCT CCCCTCACTG
    AGCGCATTTC TCAATGCTGA CAGAAAGGTA TCACCCCAGC CCTTGTGGTT TTCTGAAGAA
    CTTGCCAATT TGCGTAAGAT TCATGAACTT CCTTATCACC TTGCTCACGC AGGAAAATGG
    GACGAGCTAC GACAATCAGT GCTTGGGAGT CTGGACTGGT TGTGCTGTAA GACTCTGCAG
    TGTGGCGTGA GCTGTGTTAT TCAGGACCTG ACCCTTTGCA CTGACCTCAC TGACTGCCCT
    GAGATACAGT TGCTGAAGGA GACGTTTTTG CTTCTTAAAC CCTCGCTGGA TTTTAGCGAC
    GGCCGCGTGG ATCCCTCGCT GTTAGTGACA GAGATCTTTG CCAGGCTACA GGGTCTTGCT
    GATCATTACC CCTCCATGAT TGGTCAACTG TGTTCTCAAT GTATGGACTG GTTTGAATCC
    TATTCTGACC CATTACTTGT GCCCAAATGC AGCTTCTTTG AAGCCCCTGG TGGCCCACTA
    AAGAGCACCC TCAGAGGATT CACTAAAGGG GTGAAGGCTG TTGTTCTGTG CTCAAAGAGG
    AAGCTGCTGT TAGCAGGTTC AGAGGATGGA CTGATCATAG TCTGGAGTCT AAACAATCTG
    CAGCCTCTTC ACACTCTTTC TGGACATAAA GCCGGGGTGC TGTCCCTGAA GCTGACGGAC
    AAAACCAATA ACTGTTTGTC TACTGGGCTC GATGGGACTC TGAGAAAATG GTGTCTGATA
    AGTGGACTGC AGCTTTTCTG CATCACTGAT GCCGTTTCTG TCCAAACACA CAGCCCTACA
    CACATCCATA TGAATGAAGA AAGGAAGATC ATCATAACCA ACCCAAGCAG CCAAGTTAAA
    GCTTGGGATT TGGACACTGC AGAGCCTCTG TTTGAGCTGA CCGCAGTGAT TGGTTCTGTT
    CTTGGCATTG TGGAAGATGA AATAGCGTGC ATATGCAGTG AGGCGATACT CTGCTTTTAT
    GACATATCCA CCGGCACTCA GACTTCCCAG ACCCTCCTGC CCTACTCTGC CGGACACCAT
    AACATCACCT GCTGTCTCAC ACTCACCAAA CACTCCAAGA TACTCATTGC TTTTGGGGAA
    AGCCGTCTAC ATTCGGTGTG GAGCCATGAA ATGGAGTCTG TGATTGATCT TCCATCTCCA
    GCTCTCTTTC TCAGAACATC TGAGGATGAA AAACTGCTGC TTGCAGGATG TGACAGAACA
    TTGTGCGTGT TCCTCGTGAC ATTGACTTCA ATGCACAAGG TTCTGGATCT TCAACATGAT
    GCATCTATCC TGTGCGCCGT GTCAAACAGC GAAGGTAGCG AAATCATCAC GAGTGCCCAG
    GATCGTATAA TACGGGTATG GAGCGTAACC ACAGGAGCCC TGCTGGACTG GATCGGTGTG
    ATGGATTCCT CTGTGTCCTC ATTGGCTGTA CATCAGCGCT TTATTATTTC CGCCTCTGTT
    ATTGTCAACC AGCTTAAAGT ATGGCAGCTT GACCACAACA CCACGCACAG GAACAAGAGT
    TTCATCCCCG CATACTGCCC CTTGGCAGTC CTGTCGAAAG ATGGTGATAC TGTATTCTAT
    GTCAAGGAGG GAAATAAAAC CGAGGTTTTC ACCTGGAGCT GTTCGGAAGG TCTGCAGTTA
    GACAGCATGG ACGTTTCCTC AGAGGTGTGC TGTATGGAAT TAGCCCAGCA GAAACGGCTC
    CTCTTCTGCG GTCTGCACAC TGGAACCATC TTTATTTACC CGCTTGACTT TATCCCAGAA
    ACCTTGTGCC TACCGCCTCC GGAAAGCCTA CCAATGGTAC GCAGCATGGC CATCAGCCCA
    CGAGAGGACA AAATGGCCGT CGCATATGAG GATGCCGTCT GTCTGTTTGA GATTACAGCG
    AGGGACAGCT TCCCTTGCGT CGATGGACCA TTTGAAAGGT ACTCGCTTGA CCTGTTACAT
    TCCTCAATCT CCGCCATGGC TTTGTTGTCC GACTGCAGGC TGCTGTATGG GACGTTTGGC
    GGCGAAGTGG TGATCTACGA CTTTAAAAGC GCCACAGCAG CAGAGCTGGA TCACCACGGT
    GCAGCCATAA CCTGCATCAC CATGAGCAAC TGGGATGCGC AGGCACTGAT TGGCTCTCAG
    GACTGTGTTC AGAAGCTGTG GAATTTGAGT CCCGTCTTAC TGAATCACAC CATGGAGTAC
    AAGGGCTTTT ATTTTGAAGG AGTTCTATGT GCTGTCTTCT CAATAAATGA TAAATACGTG
    TTCACTGGAT CCTTGGACAA AACCGTCAAA GTCTGGGATG TTTCGAGTGG TTGTTTGCTG
    TATATCCAGT ACGTCTACTC TCCGGTCATA AGGATGATGC CACACAAGGA TGGATTTGTA
    GCTGTATCCC AGCATGGCTC ATTCATCAAG GAAGGTTTTC GCTGTCCAGA CAGCCTCAAG
    CCTGGATATA ACCCCCTCCG GAACTTCCAA GTACATTACA AAGTCACTTC ACGAGACAAA
    AGTCTGAACG TTCCACACAG CGTGATCAAT GAGCTGCCAG ATTACAACCC TGCTCAGTTC
    AACTTCATTG GCATCGGTCT GATCAAATCC AAACCATCCA ATACGTGCGT TCTCCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002663751.3
    CDS520..5379
    Translation

    Target ORF information:

    RefSeq Version XM_002663705.6
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT and WD repeat domain containing 1 (nwd1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002663705.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGATGTGG TGGAGATGAG AGAGGCACTT GGGTTGGAGG TTCTGCGGGG ACAAAGCGCT 
    GATTCTCTGC AGCCTACTTC CAGAATGGTG CGGGTGTTCA TCAGCTCCAC TCACTCAGAC
    ATGAAAAATG AGAGAAATGC ATTCTGGGAG AAGACCTATT CGGAGCTACA GACCCACTGC
    CAAAGTTTGG GTCTTGTTTT TGAGGTAGTG GATTTCAAAT GTGGAATGCG TGACATCACT
    GCATCTGATC ATTTAATAAC TGAACTGTGC ATTAAAGAAA TCGAAACCTG CCAGAGGATA
    TCAGATGGGC CAACTTTTAT AGCTTTGATC GGTAATCAGT ATGGCCATCG CCTTGTCCCG
    CGCCTCATAC CAGAGGCAGA GTTTGAGTTG CTGCTATCAA AACTTTCCAA AGATCCAGAG
    AGTTTAAAGC TCCTTAACCA ATGGTTTTAC AAGGACAGTA ACTCAATCCC ACCCACATAT
    ATCCTCCTGC CAATCACGGT GCACTACCCA CATTACAATG AGAGAGATTC TGAGAACGCA
    CAGTTGCGGG ATAATGATGT CATATCCTGG AATCTAACAG AGTCACGGCT GCTGCAGGTT
    CTCCGCAGTG CAGCTCTACA GGCTGAGAGA GACGGGGATT TCACTGCTGA CAGCAAGCAC
    AAGTTTTTCA AATCTGTTTT AGAATATGAG ATGGAGCATG GCTTTCTCAG GACTCAAAGG
    GATGCATCGG CTGTGCTGTT TTTCCGCGAA CTGCCTCGAC TTAGTAAAAG AGACTGCAAG
    AAAAACTTTG CAAAGTTTCT GCTGGACGTC ACAGCCGACG GCTTGCTGGA CACACAAGCC
    CGGGAGCTCT TGACTGACCT GAAGCAACGC ATCTGCAACA CCGGTCGCCT CCAACCACTC
    TGTATGGAGC TCAGCAAAGG AGCCATGGAC CCAAGCCGCA AAGAACACAA GGAATACCTG
    GGTAAGCTGT GTGAACAGGT AACATCTCAA TTGAAGGAAC TGATCAGCAG AAATGCTCTG
    CAAACCTCAG GAACTTCAGT AGATCCAGTA TGGTCCTGGC TAAAACAGGA GATCTTTCAT
    CATGCCAAGT TGAGTGAGGA GAAGTGTGCT GTGTTTAAAG GGAGAGACGA CATTCTGGGA
    AAGATCTCCA TCACCCTGTG GGAATCCAAT GATGTTTCTC ATGGACCTCT TCTAGTCCAT
    GGACCTCCAG GAGTTGGTAA AACAGCTCTG CTTTGCAAAC TGGCCAAGGA AATGCGAGCA
    GTTGTTGACA ATCGGGGAGT TGTTCTGCTG AGGTTGCTGG GAACATCTCT GCTCAGTTCA
    GATGTCGATT CTGTTCTTCG AGGTGTTTGT CTTCAAGTGT GTGGAGCTCT AGGGTTGCAG
    TCCCCATACT CGCATATAGC GTACACGCAT GAAGGGCTAG TTCGATTTTT CCATTCCATG
    CTGGAAGACG TGTCAAAACA AGGAGACACT CTTCTACTCA TTCTAGACTC ATTGGAGCAG
    CTTTTAGAGA CAAACAAACT TCATAAACTA GACTGGATAC CTAAAATAAT TCCACCCAAT
    GTGCAAATCG TATTCTCAAC TTCTAATGAG TGCTTGGACA AATGTAAATC TTTGGTTTCA
    GCGGAAGAAA ACATCTTTAA ATTGGAACTT CTCACGAAAG ATCAAGGGAG AGACGTCATT
    GATGCATATA TGAGTGCAGC TGAACGCAAG CTGACGGCAG AACAGCAAGA CGCAATCTTA
    CGCTACTTTG AGCAAAGTGG AAACCCTTTG CATCTGAAAC TCATGCTAGA CACTGCCAAA
    CTGTGGTCCT CCTACACCCC CATGTCTAGT GTTCACCTGG ACAAGTCGGC GGAGGAAGTG
    ATGACACAGT TTGTCGAGTC ACTTGAGGTG AATCATGGGA AGAAGCTAGT CAGTCATGCT
    CTGAGCTTCA TCCTGTTATC AAGATATGGA CTTTCAGAGG CAGAGTTACA GGATGTCCTA
    TCATTAGATA ATGATGTTCT AGCTGAGATC TATAAATGCT GGCTTCCTCC GAGCCATACG
    CTCATGCGCT TCCCACGTTT TCATTGGTCA AGACTCAGGC ATGACCTGAG TGCTCATCTG
    ACGGAGAGAT GGGAAGCCGG CGTTCTTCTG CTGGGTTTCA CTCACAGGCA GTTTGCAGAA
    CTGGTAAGGA GGAGATATTT GTCACGTGAT GTGAAAGCTG ACATGCACAC GATCCTCGCC
    GAGTATTTCT CCGGACAGTG GAGTAAGGGG CAGCTCAGAC CCATCCTTCT CCCCTCACTG
    AGCGCATTTC TCAATGCTGA CAGAAAGGTA TCACCCCAGC CCTTGTGGTT TTCTGAAGAA
    CTTGCCAATT TGCGTAAGAT TCATGAACTT CCTTATCACC TTGCTCACGC AGGAAAATGG
    GACGAGCTAC GACAATCAGT GCTTGGGAGT CTGGACTGGT TGTGCTGTAA GACTCTGCAG
    TGTGGCGTGA GCTGTGTTAT TCAGGACCTG ACCCTTTGCA CTGACCTCAC TGACTGCCCT
    GAGATACAGT TGCTGAAGGA GACGTTTTTG CTTCTTAAAC CCTCGCTGGA TTTTAGCGAC
    GGCCGCGTGG ATCCCTCGCT GTTAGTGACA GAGATCTTTG CCAGGCTACA GGGTCTTGCT
    GATCATTACC CCTCCATGAT TGGTCAACTG TGTTCTCAAT GTATGGACTG GTTTGAATCC
    TATTCTGACC CATTACTTGT GCCCAAATGC AGCTTCTTTG AAGCCCCTGG TGGCCCACTA
    AAGAGCACCC TCAGAGGATT CACTAAAGGG GTGAAGGCTG TTGTTCTGTG CTCAAAGAGG
    AAGCTGCTGT TAGCAGGTTC AGAGGATGGA CTGATCATAG TCTGGAGTCT AAACAATCTG
    CAGCCTCTTC ACACTCTTTC TGGACATAAA GCCGGGGTGC TGTCCCTGAA GCTGACGGAC
    AAAACCAATA ACTGTTTGTC TACTGGGCTC GATGGGACTC TGAGAAAATG GTGTCTGATA
    AGTGGACTGC AGCTTTTCTG CATCACTGAT GCCGTTTCTG TCCAAACACA CAGCCCTACA
    CACATCCATA TGAATGAAGA AAGGAAGATC ATCATAACCA ACCCAAGCAG CCAAGTTAAA
    GCTTGGGATT TGGACACTGC AGAGCCTCTG TTTGAGCTGA CCGCAGTGAT TGGTTCTGTT
    CTTGGCATTG TGGAAGATGA AATAGCGTGC ATATGCAGTG AGGCGATACT CTGCTTTTAT
    GACATATCCA CCGGCACTCA GACTTCCCAG ACCCTCCTGC CCTACTCTGC CGGACACCAT
    AACATCACCT GCTGTCTCAC ACTCACCAAA CACTCCAAGA TACTCATTGC TTTTGGGGAA
    AGCCGTCTAC ATTCGGTGTG GAGCCATGAA ATGGAGTCTG TGATTGATCT TCCATCTCCA
    GCTCTCTTTC TCAGAACATC TGAGGATGAA AAACTGCTGC TTGCAGGATG TGACAGAACA
    TTGTGCGTGT TCCTCGTGAC ATTGACTTCA ATGCACAAGG TTCTGGATCT TCAACATGAT
    GCATCTATCC TGTGCGCCGT GTCAAACAGC GAAGGTAGCG AAATCATCAC GAGTGCCCAG
    GATCGTATAA TACGGGTATG GAGCGTAACC ACAGGAGCCC TGCTGGACTG GATCGGTGTG
    ATGGATTCCT CTGTGTCCTC ATTGGCTGTA CATCAGCGCT TTATTATTTC CGCCTCTGTT
    ATTGTCAACC AGCTTAAAGT ATGGCAGCTT GACCACAACA CCACGCACAG GAACAAGAGT
    TTCATCCCCG CATACTGCCC CTTGGCAGTC CTGTCGAAAG ATGGTGATAC TGTATTCTAT
    GTCAAGGAGG GAAATAAAAC CGAGGTTTTC ACCTGGAGCT GTTCGGAAGG TCTGCAGTTA
    GACAGCATGG ACGTTTCCTC AGAGGTGTGC TGTATGGAAT TAGCCCAGCA GAAACGGCTC
    CTCTTCTGCG GTCTGCACAC TGGAACCATC TTTATTTACC CGCTTGACTT TATCCCAGAA
    ACCTTGTGCC TACCGCCTCC GGAAAGCCTA CCAATGGTAC GCAGCATGGC CATCAGCCCA
    CGAGAGGACA AAATGGCCGT CGCATATGAG GATGCCGTCT GTCTGTTTGA GATTACAGCG
    AGGGACAGCT TCCCTTGCGT CGATGGACCA TTTGAAAGGT ACTCGCTTGA CCTGTTACAT
    TCCTCAATCT CCGCCATGGC TTTGTTGTCC GACTGCAGGC TGCTGTATGG GACGTTTGGC
    GGCGAAGTGG TGATCTACGA CTTTAAAAGC GCCACAGCAG CAGAGCTGGA TCACCACGGT
    GCAGCCATAA CCTGCATCAC CATGAGCAAC TGGGATGCGC AGGCACTGAT TGGCTCTCAG
    GACTGTGTTC AGAAGCTGTG GAATTTGAGT CCCGTCTTAC TGAATCACAC CATGGAGTAC
    AAGGGCTTTT ATTTTGAAGG AGTTCTATGT GCTGTCTTCT CAATAAATGA TAAATACGTG
    TTCACTGGAT CCTTGGACAA AACCGTCAAA GTCTGGGATG TTTCGAGTGG TTGTTTGCTG
    TATATCCAGT ACGTCTACTC TCCGGTCATA AGGATGATGC CACACAAGGA TGGATTTGTA
    GCTGTATCCC AGCATGGCTC ATTCATCAAG GAAGGTTTTC GCTGTCCAGA CAGCCTCAAG
    CCTGGATATA ACCCCCTCCG GAACTTCCAA GTACATTACA AAGTCACTTC ACGAGACAAA
    AGTCTGAACG TTCCACACAG CGTGATCAAT GAGCTGCCAG ATTACAACCC TGCTCAGTTC
    AACTTCATTG GCATCGGTCT GATCAAATCC AAACCATCCA ATACGTGCGT TCTCCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa16761
    Clone ID Related Accession (Same CDS sequence) XM_002663705.6 , XM_002663705.5
    Accession Version XM_002663705.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NACHT domain- and WD repeat-containing protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002663705.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGATGTGG TGGAGATGAG AGAGGCACTT GGGTTGGAGG TTCTGCGGGG ACAAAGCGCT 
    GATTCTCTGC AGCCTACTTC CAGAATGGTG CGGGTGTTCA TCAGCTCCAC TCACTCAGAC
    ATGAAAAATG AGAGAAATGC ATTCTGGGAG AAGACCTATT CGGAGCTACA GACCCACTGC
    CAAAGTTTGG GTCTTGTTTT TGAGGTAGTG GATTTCAAAT GTGGAATGCG TGACATCACT
    GCATCTGATC ATTTAATAAC TGAACTGTGC ATTAAAGAAA TCGAAACCTG CCAGAGGATA
    TCAGATGGGC CAACTTTTAT AGCTTTGATC GGTAATCAGT ATGGCCATCG CCTTGTCCCG
    CGCCTCATAC CAGAGGCAGA GTTTGAGTTG CTGCTATCAA AACTTTCCAA AGATCCAGAG
    AGTTTAAAGC TCCTTAACCA ATGGTTTTAC AAGGACAGTA ACTCAATCCC ACCCACATAT
    ATCCTCCTGC CAATCACGGT GCACTACCCA CATTACAATG AGAGAGATTC TGAGAACGCA
    CAGTTGCGGG ATAATGATGT CATATCCTGG AATCTAACAG AGTCACGGCT GCTGCAGGTT
    CTCCGCAGTG CAGCTCTACA GGCTGAGAGA GACGGGGATT TCACTGCTGA CAGCAAGCAC
    AAGTTTTTCA AATCTGTTTT AGAATATGAG ATGGAGCATG GCTTTCTCAG GACTCAAAGG
    GATGCATCGG CTGTGCTGTT TTTCCGCGAA CTGCCTCGAC TTAGTAAAAG AGACTGCAAG
    AAAAACTTTG CAAAGTTTCT GCTGGACGTC ACAGCCGACG GCTTGCTGGA CACACAAGCC
    CGGGAGCTCT TGACTGACCT GAAGCAACGC ATCTGCAACA CCGGTCGCCT CCAACCACTC
    TGTATGGAGC TCAGCAAAGG AGCCATGGAC CCAAGCCGCA AAGAACACAA GGAATACCTG
    GGTAAGCTGT GTGAACAGGT AACATCTCAA TTGAAGGAAC TGATCAGCAG AAATGCTCTG
    CAAACCTCAG GAACTTCAGT AGATCCAGTA TGGTCCTGGC TAAAACAGGA GATCTTTCAT
    CATGCCAAGT TGAGTGAGGA GAAGTGTGCT GTGTTTAAAG GGAGAGACGA CATTCTGGGA
    AAGATCTCCA TCACCCTGTG GGAATCCAAT GATGTTTCTC ATGGACCTCT TCTAGTCCAT
    GGACCTCCAG GAGTTGGTAA AACAGCTCTG CTTTGCAAAC TGGCCAAGGA AATGCGAGCA
    GTTGTTGACA ATCGGGGAGT TGTTCTGCTG AGGTTGCTGG GAACATCTCT GCTCAGTTCA
    GATGTCGATT CTGTTCTTCG AGGTGTTTGT CTTCAAGTGT GTGGAGCTCT AGGGTTGCAG
    TCCCCATACT CGCATATAGC GTACACGCAT GAAGGGCTAG TTCGATTTTT CCATTCCATG
    CTGGAAGACG TGTCAAAACA AGGAGACACT CTTCTACTCA TTCTAGACTC ATTGGAGCAG
    CTTTTAGAGA CAAACAAACT TCATAAACTA GACTGGATAC CTAAAATAAT TCCACCCAAT
    GTGCAAATCG TATTCTCAAC TTCTAATGAG TGCTTGGACA AATGTAAATC TTTGGTTTCA
    GCGGAAGAAA ACATCTTTAA ATTGGAACTT CTCACGAAAG ATCAAGGGAG AGACGTCATT
    GATGCATATA TGAGTGCAGC TGAACGCAAG CTGACGGCAG AACAGCAAGA CGCAATCTTA
    CGCTACTTTG AGCAAAGTGG AAACCCTTTG CATCTGAAAC TCATGCTAGA CACTGCCAAA
    CTGTGGTCCT CCTACACCCC CATGTCTAGT GTTCACCTGG ACAAGTCGGC GGAGGAAGTG
    ATGACACAGT TTGTCGAGTC ACTTGAGGTG AATCATGGGA AGAAGCTAGT CAGTCATGCT
    CTGAGCTTCA TCCTGTTATC AAGATATGGA CTTTCAGAGG CAGAGTTACA GGATGTCCTA
    TCATTAGATA ATGATGTTCT AGCTGAGATC TATAAATGCT GGCTTCCTCC GAGCCATACG
    CTCATGCGCT TCCCACGTTT TCATTGGTCA AGACTCAGGC ATGACCTGAG TGCTCATCTG
    ACGGAGAGAT GGGAAGCCGG CGTTCTTCTG CTGGGTTTCA CTCACAGGCA GTTTGCAGAA
    CTGGTAAGGA GGAGATATTT GTCACGTGAT GTGAAAGCTG ACATGCACAC GATCCTCGCC
    GAGTATTTCT CCGGACAGTG GAGTAAGGGG CAGCTCAGAC CCATCCTTCT CCCCTCACTG
    AGCGCATTTC TCAATGCTGA CAGAAAGGTA TCACCCCAGC CCTTGTGGTT TTCTGAAGAA
    CTTGCCAATT TGCGTAAGAT TCATGAACTT CCTTATCACC TTGCTCACGC AGGAAAATGG
    GACGAGCTAC GACAATCAGT GCTTGGGAGT CTGGACTGGT TGTGCTGTAA GACTCTGCAG
    TGTGGCGTGA GCTGTGTTAT TCAGGACCTG ACCCTTTGCA CTGACCTCAC TGACTGCCCT
    GAGATACAGT TGCTGAAGGA GACGTTTTTG CTTCTTAAAC CCTCGCTGGA TTTTAGCGAC
    GGCCGCGTGG ATCCCTCGCT GTTAGTGACA GAGATCTTTG CCAGGCTACA GGGTCTTGCT
    GATCATTACC CCTCCATGAT TGGTCAACTG TGTTCTCAAT GTATGGACTG GTTTGAATCC
    TATTCTGACC CATTACTTGT GCCCAAATGC AGCTTCTTTG AAGCCCCTGG TGGCCCACTA
    AAGAGCACCC TCAGAGGATT CACTAAAGGG GTGAAGGCTG TTGTTCTGTG CTCAAAGAGG
    AAGCTGCTGT TAGCAGGTTC AGAGGATGGA CTGATCATAG TCTGGAGTCT AAACAATCTG
    CAGCCTCTTC ACACTCTTTC TGGACATAAA GCCGGGGTGC TGTCCCTGAA GCTGACGGAC
    AAAACCAATA ACTGTTTGTC TACTGGGCTC GATGGGACTC TGAGAAAATG GTGTCTGATA
    AGTGGACTGC AGCTTTTCTG CATCACTGAT GCCGTTTCTG TCCAAACACA CAGCCCTACA
    CACATCCATA TGAATGAAGA AAGGAAGATC ATCATAACCA ACCCAAGCAG CCAAGTTAAA
    GCTTGGGATT TGGACACTGC AGAGCCTCTG TTTGAGCTGA CCGCAGTGAT TGGTTCTGTT
    CTTGGCATTG TGGAAGATGA AATAGCGTGC ATATGCAGTG AGGCGATACT CTGCTTTTAT
    GACATATCCA CCGGCACTCA GACTTCCCAG ACCCTCCTGC CCTACTCTGC CGGACACCAT
    AACATCACCT GCTGTCTCAC ACTCACCAAA CACTCCAAGA TACTCATTGC TTTTGGGGAA
    AGCCGTCTAC ATTCGGTGTG GAGCCATGAA ATGGAGTCTG TGATTGATCT TCCATCTCCA
    GCTCTCTTTC TCAGAACATC TGAGGATGAA AAACTGCTGC TTGCAGGATG TGACAGAACA
    TTGTGCGTGT TCCTCGTGAC ATTGACTTCA ATGCACAAGG TTCTGGATCT TCAACATGAT
    GCATCTATCC TGTGCGCCGT GTCAAACAGC GAAGGTAGCG AAATCATCAC GAGTGCCCAG
    GATCGTATAA TACGGGTATG GAGCGTAACC ACAGGAGCCC TGCTGGACTG GATCGGTGTG
    ATGGATTCCT CTGTGTCCTC ATTGGCTGTA CATCAGCGCT TTATTATTTC CGCCTCTGTT
    ATTGTCAACC AGCTTAAAGT ATGGCAGCTT GACCACAACA CCACGCACAG GAACAAGAGT
    TTCATCCCCG CATACTGCCC CTTGGCAGTC CTGTCGAAAG ATGGTGATAC TGTATTCTAT
    GTCAAGGAGG GAAATAAAAC CGAGGTTTTC ACCTGGAGCT GTTCGGAAGG TCTGCAGTTA
    GACAGCATGG ACGTTTCCTC AGAGGTGTGC TGTATGGAAT TAGCCCAGCA GAAACGGCTC
    CTCTTCTGCG GTCTGCACAC TGGAACCATC TTTATTTACC CGCTTGACTT TATCCCAGAA
    ACCTTGTGCC TACCGCCTCC GGAAAGCCTA CCAATGGTAC GCAGCATGGC CATCAGCCCA
    CGAGAGGACA AAATGGCCGT CGCATATGAG GATGCCGTCT GTCTGTTTGA GATTACAGCG
    AGGGACAGCT TCCCTTGCGT CGATGGACCA TTTGAAAGGT ACTCGCTTGA CCTGTTACAT
    TCCTCAATCT CCGCCATGGC TTTGTTGTCC GACTGCAGGC TGCTGTATGG GACGTTTGGC
    GGCGAAGTGG TGATCTACGA CTTTAAAAGC GCCACAGCAG CAGAGCTGGA TCACCACGGT
    GCAGCCATAA CCTGCATCAC CATGAGCAAC TGGGATGCGC AGGCACTGAT TGGCTCTCAG
    GACTGTGTTC AGAAGCTGTG GAATTTGAGT CCCGTCTTAC TGAATCACAC CATGGAGTAC
    AAGGGCTTTT ATTTTGAAGG AGTTCTATGT GCTGTCTTCT CAATAAATGA TAAATACGTG
    TTCACTGGAT CCTTGGACAA AACCGTCAAA GTCTGGGATG TTTCGAGTGG TTGTTTGCTG
    TATATCCAGT ACGTCTACTC TCCGGTCATA AGGATGATGC CACACAAGGA TGGATTTGTA
    GCTGTATCCC AGCATGGCTC ATTCATCAAG GAAGGTTTTC GCTGTCCAGA CAGCCTCAAG
    CCTGGATATA ACCCCCTCCG GAACTTCCAA GTACATTACA AAGTCACTTC ACGAGACAAA
    AGTCTGAACG TTCCACACAG CGTGATCAAT GAGCTGCCAG ATTACAACCC TGCTCAGTTC
    AACTTCATTG GCATCGGTCT GATCAAATCC AAACCATCCA ATACGTGCGT TCTCCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002663751.3
    CDS524..5383
    Translation

    Target ORF information:

    RefSeq Version XM_002663705.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT and WD repeat domain containing 1 (nwd1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002663705.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGATGTGG TGGAGATGAG AGAGGCACTT GGGTTGGAGG TTCTGCGGGG ACAAAGCGCT 
    GATTCTCTGC AGCCTACTTC CAGAATGGTG CGGGTGTTCA TCAGCTCCAC TCACTCAGAC
    ATGAAAAATG AGAGAAATGC ATTCTGGGAG AAGACCTATT CGGAGCTACA GACCCACTGC
    CAAAGTTTGG GTCTTGTTTT TGAGGTAGTG GATTTCAAAT GTGGAATGCG TGACATCACT
    GCATCTGATC ATTTAATAAC TGAACTGTGC ATTAAAGAAA TCGAAACCTG CCAGAGGATA
    TCAGATGGGC CAACTTTTAT AGCTTTGATC GGTAATCAGT ATGGCCATCG CCTTGTCCCG
    CGCCTCATAC CAGAGGCAGA GTTTGAGTTG CTGCTATCAA AACTTTCCAA AGATCCAGAG
    AGTTTAAAGC TCCTTAACCA ATGGTTTTAC AAGGACAGTA ACTCAATCCC ACCCACATAT
    ATCCTCCTGC CAATCACGGT GCACTACCCA CATTACAATG AGAGAGATTC TGAGAACGCA
    CAGTTGCGGG ATAATGATGT CATATCCTGG AATCTAACAG AGTCACGGCT GCTGCAGGTT
    CTCCGCAGTG CAGCTCTACA GGCTGAGAGA GACGGGGATT TCACTGCTGA CAGCAAGCAC
    AAGTTTTTCA AATCTGTTTT AGAATATGAG ATGGAGCATG GCTTTCTCAG GACTCAAAGG
    GATGCATCGG CTGTGCTGTT TTTCCGCGAA CTGCCTCGAC TTAGTAAAAG AGACTGCAAG
    AAAAACTTTG CAAAGTTTCT GCTGGACGTC ACAGCCGACG GCTTGCTGGA CACACAAGCC
    CGGGAGCTCT TGACTGACCT GAAGCAACGC ATCTGCAACA CCGGTCGCCT CCAACCACTC
    TGTATGGAGC TCAGCAAAGG AGCCATGGAC CCAAGCCGCA AAGAACACAA GGAATACCTG
    GGTAAGCTGT GTGAACAGGT AACATCTCAA TTGAAGGAAC TGATCAGCAG AAATGCTCTG
    CAAACCTCAG GAACTTCAGT AGATCCAGTA TGGTCCTGGC TAAAACAGGA GATCTTTCAT
    CATGCCAAGT TGAGTGAGGA GAAGTGTGCT GTGTTTAAAG GGAGAGACGA CATTCTGGGA
    AAGATCTCCA TCACCCTGTG GGAATCCAAT GATGTTTCTC ATGGACCTCT TCTAGTCCAT
    GGACCTCCAG GAGTTGGTAA AACAGCTCTG CTTTGCAAAC TGGCCAAGGA AATGCGAGCA
    GTTGTTGACA ATCGGGGAGT TGTTCTGCTG AGGTTGCTGG GAACATCTCT GCTCAGTTCA
    GATGTCGATT CTGTTCTTCG AGGTGTTTGT CTTCAAGTGT GTGGAGCTCT AGGGTTGCAG
    TCCCCATACT CGCATATAGC GTACACGCAT GAAGGGCTAG TTCGATTTTT CCATTCCATG
    CTGGAAGACG TGTCAAAACA AGGAGACACT CTTCTACTCA TTCTAGACTC ATTGGAGCAG
    CTTTTAGAGA CAAACAAACT TCATAAACTA GACTGGATAC CTAAAATAAT TCCACCCAAT
    GTGCAAATCG TATTCTCAAC TTCTAATGAG TGCTTGGACA AATGTAAATC TTTGGTTTCA
    GCGGAAGAAA ACATCTTTAA ATTGGAACTT CTCACGAAAG ATCAAGGGAG AGACGTCATT
    GATGCATATA TGAGTGCAGC TGAACGCAAG CTGACGGCAG AACAGCAAGA CGCAATCTTA
    CGCTACTTTG AGCAAAGTGG AAACCCTTTG CATCTGAAAC TCATGCTAGA CACTGCCAAA
    CTGTGGTCCT CCTACACCCC CATGTCTAGT GTTCACCTGG ACAAGTCGGC GGAGGAAGTG
    ATGACACAGT TTGTCGAGTC ACTTGAGGTG AATCATGGGA AGAAGCTAGT CAGTCATGCT
    CTGAGCTTCA TCCTGTTATC AAGATATGGA CTTTCAGAGG CAGAGTTACA GGATGTCCTA
    TCATTAGATA ATGATGTTCT AGCTGAGATC TATAAATGCT GGCTTCCTCC GAGCCATACG
    CTCATGCGCT TCCCACGTTT TCATTGGTCA AGACTCAGGC ATGACCTGAG TGCTCATCTG
    ACGGAGAGAT GGGAAGCCGG CGTTCTTCTG CTGGGTTTCA CTCACAGGCA GTTTGCAGAA
    CTGGTAAGGA GGAGATATTT GTCACGTGAT GTGAAAGCTG ACATGCACAC GATCCTCGCC
    GAGTATTTCT CCGGACAGTG GAGTAAGGGG CAGCTCAGAC CCATCCTTCT CCCCTCACTG
    AGCGCATTTC TCAATGCTGA CAGAAAGGTA TCACCCCAGC CCTTGTGGTT TTCTGAAGAA
    CTTGCCAATT TGCGTAAGAT TCATGAACTT CCTTATCACC TTGCTCACGC AGGAAAATGG
    GACGAGCTAC GACAATCAGT GCTTGGGAGT CTGGACTGGT TGTGCTGTAA GACTCTGCAG
    TGTGGCGTGA GCTGTGTTAT TCAGGACCTG ACCCTTTGCA CTGACCTCAC TGACTGCCCT
    GAGATACAGT TGCTGAAGGA GACGTTTTTG CTTCTTAAAC CCTCGCTGGA TTTTAGCGAC
    GGCCGCGTGG ATCCCTCGCT GTTAGTGACA GAGATCTTTG CCAGGCTACA GGGTCTTGCT
    GATCATTACC CCTCCATGAT TGGTCAACTG TGTTCTCAAT GTATGGACTG GTTTGAATCC
    TATTCTGACC CATTACTTGT GCCCAAATGC AGCTTCTTTG AAGCCCCTGG TGGCCCACTA
    AAGAGCACCC TCAGAGGATT CACTAAAGGG GTGAAGGCTG TTGTTCTGTG CTCAAAGAGG
    AAGCTGCTGT TAGCAGGTTC AGAGGATGGA CTGATCATAG TCTGGAGTCT AAACAATCTG
    CAGCCTCTTC ACACTCTTTC TGGACATAAA GCCGGGGTGC TGTCCCTGAA GCTGACGGAC
    AAAACCAATA ACTGTTTGTC TACTGGGCTC GATGGGACTC TGAGAAAATG GTGTCTGATA
    AGTGGACTGC AGCTTTTCTG CATCACTGAT GCCGTTTCTG TCCAAACACA CAGCCCTACA
    CACATCCATA TGAATGAAGA AAGGAAGATC ATCATAACCA ACCCAAGCAG CCAAGTTAAA
    GCTTGGGATT TGGACACTGC AGAGCCTCTG TTTGAGCTGA CCGCAGTGAT TGGTTCTGTT
    CTTGGCATTG TGGAAGATGA AATAGCGTGC ATATGCAGTG AGGCGATACT CTGCTTTTAT
    GACATATCCA CCGGCACTCA GACTTCCCAG ACCCTCCTGC CCTACTCTGC CGGACACCAT
    AACATCACCT GCTGTCTCAC ACTCACCAAA CACTCCAAGA TACTCATTGC TTTTGGGGAA
    AGCCGTCTAC ATTCGGTGTG GAGCCATGAA ATGGAGTCTG TGATTGATCT TCCATCTCCA
    GCTCTCTTTC TCAGAACATC TGAGGATGAA AAACTGCTGC TTGCAGGATG TGACAGAACA
    TTGTGCGTGT TCCTCGTGAC ATTGACTTCA ATGCACAAGG TTCTGGATCT TCAACATGAT
    GCATCTATCC TGTGCGCCGT GTCAAACAGC GAAGGTAGCG AAATCATCAC GAGTGCCCAG
    GATCGTATAA TACGGGTATG GAGCGTAACC ACAGGAGCCC TGCTGGACTG GATCGGTGTG
    ATGGATTCCT CTGTGTCCTC ATTGGCTGTA CATCAGCGCT TTATTATTTC CGCCTCTGTT
    ATTGTCAACC AGCTTAAAGT ATGGCAGCTT GACCACAACA CCACGCACAG GAACAAGAGT
    TTCATCCCCG CATACTGCCC CTTGGCAGTC CTGTCGAAAG ATGGTGATAC TGTATTCTAT
    GTCAAGGAGG GAAATAAAAC CGAGGTTTTC ACCTGGAGCT GTTCGGAAGG TCTGCAGTTA
    GACAGCATGG ACGTTTCCTC AGAGGTGTGC TGTATGGAAT TAGCCCAGCA GAAACGGCTC
    CTCTTCTGCG GTCTGCACAC TGGAACCATC TTTATTTACC CGCTTGACTT TATCCCAGAA
    ACCTTGTGCC TACCGCCTCC GGAAAGCCTA CCAATGGTAC GCAGCATGGC CATCAGCCCA
    CGAGAGGACA AAATGGCCGT CGCATATGAG GATGCCGTCT GTCTGTTTGA GATTACAGCG
    AGGGACAGCT TCCCTTGCGT CGATGGACCA TTTGAAAGGT ACTCGCTTGA CCTGTTACAT
    TCCTCAATCT CCGCCATGGC TTTGTTGTCC GACTGCAGGC TGCTGTATGG GACGTTTGGC
    GGCGAAGTGG TGATCTACGA CTTTAAAAGC GCCACAGCAG CAGAGCTGGA TCACCACGGT
    GCAGCCATAA CCTGCATCAC CATGAGCAAC TGGGATGCGC AGGCACTGAT TGGCTCTCAG
    GACTGTGTTC AGAAGCTGTG GAATTTGAGT CCCGTCTTAC TGAATCACAC CATGGAGTAC
    AAGGGCTTTT ATTTTGAAGG AGTTCTATGT GCTGTCTTCT CAATAAATGA TAAATACGTG
    TTCACTGGAT CCTTGGACAA AACCGTCAAA GTCTGGGATG TTTCGAGTGG TTGTTTGCTG
    TATATCCAGT ACGTCTACTC TCCGGTCATA AGGATGATGC CACACAAGGA TGGATTTGTA
    GCTGTATCCC AGCATGGCTC ATTCATCAAG GAAGGTTTTC GCTGTCCAGA CAGCCTCAAG
    CCTGGATATA ACCCCCTCCG GAACTTCCAA GTACATTACA AAGTCACTTC ACGAGACAAA
    AGTCTGAACG TTCCACACAG CGTGATCAAT GAGCTGCCAG ATTACAACCC TGCTCAGTTC
    AACTTCATTG GCATCGGTCT GATCAAATCC AAACCATCCA ATACGTGCGT TCTCCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49402
    Clone ID Related Accession (Same CDS sequence) XM_009305820.2 , XM_009305820.3
    Accession Version XM_009305820.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NACHT domain- and WD repeat-containing protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305820.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGATGTGG TGGAGATGAG AGAGGCACTT GGGTTGGAGG TTCTGCGGGG ACAAAGCGCT 
    GATTCTCTGC AGCCTACTTC CAGAATGGTG CGGGTGTTCA TCAGCTCCAC TCACTCAGAC
    ATGAAAAATG AGAGAAATGC ATTCTGGGAG AAGACCTATT CGGAGCTACA GACCCACTGC
    CAAAGTTTGG GTCTTGTTTT TGAGGTAGTG GATTTCAAAT GTGGAATGCG TGACATCACT
    GCATCTGATC ATTTAATAAC TGAACTGTGC ATTAAAGAAA TCGAAACCTG CCAGAGGATA
    TCAGATGGGC CAACTTTTAT AGCTTTGATC GGTAATCAGT ATGGCCATCG CCTTGTCCCG
    CGCCTCATAC CAGAGGCAGA GTTTGAGTTG CTGCTATCAA AACTTTCCAA AGATCCAGAG
    AGTTTAAAGC TCCTTAACCA ATGGTTTTAC AAGGACAGTA ACTCAATCCC ACCCACATAT
    ATCCTCCTGC CAATCACGGT GCACTACCCA CATTACAATG AGAGAGATTC TGAGAACGCA
    CAGTTGCGGG ATAATGATGT CATATCCTGG AATCTAACAG AGTCACGGCT GCTGCAGGTT
    CTCCGCAGTG CAGCTCTACA GGCTGAGAGA GACGGGGATT TCACTGCTGA CAGCAAGCAC
    AAGTTTTTCA AATCTGTTTT AGAATATGAG ATGGAGCATG GCTTTCTCAG GACTCAAAGG
    GATGCATCGG CTGTGCTGTT TTTCCGCGAA CTGCCTCGAC TTAGTAAAAG AGACTGCAAG
    AAAAACTTTG CAAAGTTTCT GCTGGACGTC ACAGCCGACG GCTTGCTGGA CACACAAGCC
    CGGGAGCTCT TGACTGACCT GAAGCAACGC ATCTGCAACA CCGGTCGCCT CCAACCACTC
    TGTATGGAGC TCAGCAAAGG AGCCATGGAC CCAAGCCGCA AAGAACACAA GGAATACCTG
    GGTAAGCTGT GTGAACAGGT AACATCTCAA TTGAAGGAAC TGATCAGCAG AAATGCTCTG
    CAAACCTCAG GAACTTCAGT AGATCCAGTA TGGTCCTGGC TAAAACAGGA GATCTTTCAT
    CATGCCAAGT TGAGTGAGGA GAAGTGTGCT GTGTTTAAAG GGAGAGACGA CATTCTGGGA
    AAGATCTCCA TCACCCTGTG GGAATCCAAT GATGTTTCTC ATGGACCTCT TCTAGTCCAT
    GGACCTCCAG GAGTTGGTAA AACAGCTCTG CTTTGCAAAC TGGCCAAGGA AATGCGAGCA
    GTTGTTGACA ATCGGGGAGT TGTTCTGCTG AGGTTGCTGG GAACATCTCT GCTCAGTTCA
    GATGTCGATT CTGTTCTTCG AGGTGTTTGT CTTCAAGTGT GTGGAGCTCT AGGGTTGCAG
    TCCCCATACT CGCATATAGC GTACACGCAT GAAGGGCTAG TTCGATTTTT CCATTCCATG
    CTGGAAGACG TGTCAAAACA AGGAGACACT CTTCTACTCA TTCTAGACTC ATTGGAGCAG
    CTTTTAGAGA CAAACAAACT TCATAAACTA GACTGGATAC CTAAAATAAT TCCACCCAAT
    GTGCAAATCG TATTCTCAAC TTCTAATGAG TGCTTGGACA AATGTAAATC TTTGGTTTCA
    GCGGAAGAAA ACATCTTTAA ATTGGAACTT CTCACGAAAG ATCAAGGGAG AGACGTCATT
    GATGCATATA TGAGTGCAGC TGAACGCAAG CTGACGGCAG AACAGCAAGA CGCAATCTTA
    CGCTACTTTG AGCAAAGTGG AAACCCTTTG CATCTGAAAC TCATGCTAGA CACTGCCAAA
    CTGTGGTCCT CCTACACCCC CATGTCTAGT GTTCACCTGG ACAAGTCGGC GGAGGAAGTG
    ATGACACAGT TTGTCGAGTC ACTTGAGGTG AATCATGGGA AGAAGCTAGT CAGTCATGCT
    CTGAGCTTCA TCCTGTTATC AAGATATGGA CTTTCAGAGG CAGAGTTACA GGATGTCCTA
    TCATTAGATA ATGATGTTCT AGCTGAGATC TATAAATGCT GGCTTCCTCC GAGCCATACG
    CTCATGCGCT TCCCACGTTT TCATTGGTCA AGACTCAGGC ATGACCTGAG TGCTCATCTG
    ACGGAGAGAT GGGAAGCCGG CGTTCTTCTG CTGGGTTTCA CTCACAGGCA GTTTGCAGAA
    CTGGTAAGGA GGAGATATTT GTCACGTGAT GTGAAAGCTG ACATGCACAC GATCCTCGCC
    GAGTATTTCT CCGGACAGTG GAGTAAGGGG CAGCTCAGAC CCATCCTTCT CCCCTCACTG
    AGCGCATTTC TCAATGCTGA CAGAAAGGTA TCACCCCAGC CCTTGTGGTT TTCTGAAGAA
    CTTGCCAATT TGCGTAAGAT TCATGAACTT CCTTATCACC TTGCTCACGC AGGAAAATGG
    GACGAGCTAC GACAATCAGT GCTTGGGAGT CTGGACTGGT TGTGCTGTAA GACTCTGCAG
    TGTGGCGTGA GCTGTGTTAT TCAGGACCTG ACCCTTTGCA CTGACCTCAC TGACTGCCCT
    GAGATACAGT TGCTGAAGGA GACGTTTTTG CTTCTTAAAC CCTCGCTGGA TTTTAGCGAC
    GGCCGCGTGG ATCCCTCGCT GTTAGTGACA GAGATCTTTG CCAGGCTACA GGGTCTTGCT
    GATCATTACC CCTCCATGAT TGGTCAACTG TGTTCTCAAT GTATGGACTG GTTTGAATCC
    TATTCTGACC CATTACTTGT GCCCAAATGC AGCTTCTTTG AAGCCCCTGG TGGCCCACTA
    AAGAGCACCC TCAGAGGATT CACTAAAGGG GTGAAGGCTG TTGTTCTGTG CTCAAAGAGG
    AAGCTGCTGT TAGCAGGTTC AGAGGATGGA CTGATCATAG TCTGGAGTCT AAACAATCTG
    CAGCCTCTTC ACACTCTTTC TGGACATAAA GCCGGGGTGC TGTCCCTGAA GCTGACGGAC
    AAAACCAATA ACTGTTTGTC TACTGGGCTC GATGGGACTC TGAGAAAATG GTGTCTGATA
    AGTGGACTGC AGCTTTTCTG CATCACTGAT GCCGTTTCTG TCCAAACACA CAGCCCTACA
    CACATCCATA TGAATGAAGA AAGGAAGATC ATCATAACCA ACCCAAGCAG CCAAGTTAAA
    GCTTGGGATT TGGACACTGC AGAGCCTCTG TTTGAGCTGA CCGCAGTGAT TGGTTCTGTT
    CTTGGCATTG TGGAAGATGA AATAGCGTGC ATATGCAGTG AGGCGATACT CTGCTTTTAT
    GACATATCCA CCGGCACTCA GACTTCCCAG ACCCTCCTGC CCTACTCTGC CGGACACCAT
    AACATCACCT GCTGTCTCAC ACTCACCAAA CACTCCAAGA TACTCATTGC TTTTGGGGAA
    AGCCGTCTAC ATTCGGTGTG GAGCCATGAA ATGGAGTCTG TGATTGATCT TCCATCTCCA
    GCTCTCTTTC TCAGAACATC TGAGGATGAA AAACTGCTGC TTGCAGGATG TGACAGAACA
    TTGTGCGTGT TCCTCGTGAC ATTGACTTCA ATGCACAAGG TTCTGGATCT TCAACATGAT
    GCATCTATCC TGTGCGCCGT GTCAAACAGC GAAGGTAGCG AAATCATCAC GAGTGCCCAG
    GATCGTATAA TACGGGTATG GAGCGTAACC ACAGGAGCCC TGCTGGACTG GATCGGTGTG
    ATGGATTCCT CTGTGTCCTC ATTGGCTGTA CATCAGCGCT TTATTATTTC CGCCTCTGTT
    ATTGTCAACC AGCTTAAAGT ATGGCAGCTT GACCACAACA CCACGCACAG GAACAAGAGT
    TTCATCCCCG CATACTGCCC CTTGGCAGTC CTGTCGAAAG ATGGTGATAC TGTATTCTAT
    GTCAAGGAGG GAAATAAAAC CGAGGTTTTC ACCTGGAGCT GTTCGGAAGG TGAAACATGT
    CTGCAGTTAG ACAGCATGGA CGTTTCCTCA GAGGTGTGCT GTATGGAATT AGCCCAGCAG
    AAACGGCTCC TCTTCTGCGG TCTGCACACT GGAACCATCT TTATTTACCC GCTTGACTTT
    ATCCCAGAAA CCTTGTGCCT ACCGCCTCCG GAAAGCCTAC CAATGGTACG CAGCATGGCC
    ATCAGCCCAC GAGAGGACAA AATGGCCGTC GCATATGAGG ATGCCGTCTG TCTGTTTGAG
    ATTACAGCGA GGGACAGCTT CCCTTGCGTC GATGGACCAT TTGAAAGGTA CTCGCTTGAC
    CTGTTACATT CCTCAATCTC CGCCATGGCT TTGTTGTCCG ACTGCAGGCT GCTGTATGGG
    ACGTTTGGCG GCGAAGTGGT GATCTACGAC TTTAAAAGCG CCACAGCAGC AGAGCTGGAT
    CACCACGGTG CAGCCATAAC CTGCATCACC ATGAGCAACT GGGATGCGCA GGCACTGATT
    GGCTCTCAGG ACTGTGTTCA GAAGCTGTGG AATTTGAGTC CCGTCTTACT GAATCACACC
    ATGGAGTACA AGGGCTTTTA TTTTGAAGGA GTTCTATGTG CTGTCTTCTC AATAAATGAT
    AAATACGTGT TCACTGGATC CTTGGACAAA ACCGTCAAAG TCTGGGATGT TTCGAGTGGT
    TGTTTGCTGT ATATCCAGTA CGTCTACTCT CCGGTCATAA GGATGATGCC ACACAAGGAT
    GGATTTGTAG CTGTATCCCA GCATGGCTCA TTCATCAAGG AAGGTTTTCG CTGTCCAGAC
    AGCCTCAAGC CTGGATATAA CCCCCTCCGG AACTTCCAAG TACATTACAA AGTCACTTCA
    CGAGACAAAA GTCTGAACGT TCCACACAGC GTGATCAATG AGCTGCCAGA TTACAACCCT
    GCTCAGTTCA ACTTCATTGG CATCGGTCTG ATCAAATCCA AACCATCCAA TACGTGCGTT
    CTCCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304095.1
    CDS524..5392
    Translation

    Target ORF information:

    RefSeq Version XM_009305820.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT and WD repeat domain containing 1 (nwd1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305820.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGATGTGG TGGAGATGAG AGAGGCACTT GGGTTGGAGG TTCTGCGGGG ACAAAGCGCT 
    GATTCTCTGC AGCCTACTTC CAGAATGGTG CGGGTGTTCA TCAGCTCCAC TCACTCAGAC
    ATGAAAAATG AGAGAAATGC ATTCTGGGAG AAGACCTATT CGGAGCTACA GACCCACTGC
    CAAAGTTTGG GTCTTGTTTT TGAGGTAGTG GATTTCAAAT GTGGAATGCG TGACATCACT
    GCATCTGATC ATTTAATAAC TGAACTGTGC ATTAAAGAAA TCGAAACCTG CCAGAGGATA
    TCAGATGGGC CAACTTTTAT AGCTTTGATC GGTAATCAGT ATGGCCATCG CCTTGTCCCG
    CGCCTCATAC CAGAGGCAGA GTTTGAGTTG CTGCTATCAA AACTTTCCAA AGATCCAGAG
    AGTTTAAAGC TCCTTAACCA ATGGTTTTAC AAGGACAGTA ACTCAATCCC ACCCACATAT
    ATCCTCCTGC CAATCACGGT GCACTACCCA CATTACAATG AGAGAGATTC TGAGAACGCA
    CAGTTGCGGG ATAATGATGT CATATCCTGG AATCTAACAG AGTCACGGCT GCTGCAGGTT
    CTCCGCAGTG CAGCTCTACA GGCTGAGAGA GACGGGGATT TCACTGCTGA CAGCAAGCAC
    AAGTTTTTCA AATCTGTTTT AGAATATGAG ATGGAGCATG GCTTTCTCAG GACTCAAAGG
    GATGCATCGG CTGTGCTGTT TTTCCGCGAA CTGCCTCGAC TTAGTAAAAG AGACTGCAAG
    AAAAACTTTG CAAAGTTTCT GCTGGACGTC ACAGCCGACG GCTTGCTGGA CACACAAGCC
    CGGGAGCTCT TGACTGACCT GAAGCAACGC ATCTGCAACA CCGGTCGCCT CCAACCACTC
    TGTATGGAGC TCAGCAAAGG AGCCATGGAC CCAAGCCGCA AAGAACACAA GGAATACCTG
    GGTAAGCTGT GTGAACAGGT AACATCTCAA TTGAAGGAAC TGATCAGCAG AAATGCTCTG
    CAAACCTCAG GAACTTCAGT AGATCCAGTA TGGTCCTGGC TAAAACAGGA GATCTTTCAT
    CATGCCAAGT TGAGTGAGGA GAAGTGTGCT GTGTTTAAAG GGAGAGACGA CATTCTGGGA
    AAGATCTCCA TCACCCTGTG GGAATCCAAT GATGTTTCTC ATGGACCTCT TCTAGTCCAT
    GGACCTCCAG GAGTTGGTAA AACAGCTCTG CTTTGCAAAC TGGCCAAGGA AATGCGAGCA
    GTTGTTGACA ATCGGGGAGT TGTTCTGCTG AGGTTGCTGG GAACATCTCT GCTCAGTTCA
    GATGTCGATT CTGTTCTTCG AGGTGTTTGT CTTCAAGTGT GTGGAGCTCT AGGGTTGCAG
    TCCCCATACT CGCATATAGC GTACACGCAT GAAGGGCTAG TTCGATTTTT CCATTCCATG
    CTGGAAGACG TGTCAAAACA AGGAGACACT CTTCTACTCA TTCTAGACTC ATTGGAGCAG
    CTTTTAGAGA CAAACAAACT TCATAAACTA GACTGGATAC CTAAAATAAT TCCACCCAAT
    GTGCAAATCG TATTCTCAAC TTCTAATGAG TGCTTGGACA AATGTAAATC TTTGGTTTCA
    GCGGAAGAAA ACATCTTTAA ATTGGAACTT CTCACGAAAG ATCAAGGGAG AGACGTCATT
    GATGCATATA TGAGTGCAGC TGAACGCAAG CTGACGGCAG AACAGCAAGA CGCAATCTTA
    CGCTACTTTG AGCAAAGTGG AAACCCTTTG CATCTGAAAC TCATGCTAGA CACTGCCAAA
    CTGTGGTCCT CCTACACCCC CATGTCTAGT GTTCACCTGG ACAAGTCGGC GGAGGAAGTG
    ATGACACAGT TTGTCGAGTC ACTTGAGGTG AATCATGGGA AGAAGCTAGT CAGTCATGCT
    CTGAGCTTCA TCCTGTTATC AAGATATGGA CTTTCAGAGG CAGAGTTACA GGATGTCCTA
    TCATTAGATA ATGATGTTCT AGCTGAGATC TATAAATGCT GGCTTCCTCC GAGCCATACG
    CTCATGCGCT TCCCACGTTT TCATTGGTCA AGACTCAGGC ATGACCTGAG TGCTCATCTG
    ACGGAGAGAT GGGAAGCCGG CGTTCTTCTG CTGGGTTTCA CTCACAGGCA GTTTGCAGAA
    CTGGTAAGGA GGAGATATTT GTCACGTGAT GTGAAAGCTG ACATGCACAC GATCCTCGCC
    GAGTATTTCT CCGGACAGTG GAGTAAGGGG CAGCTCAGAC CCATCCTTCT CCCCTCACTG
    AGCGCATTTC TCAATGCTGA CAGAAAGGTA TCACCCCAGC CCTTGTGGTT TTCTGAAGAA
    CTTGCCAATT TGCGTAAGAT TCATGAACTT CCTTATCACC TTGCTCACGC AGGAAAATGG
    GACGAGCTAC GACAATCAGT GCTTGGGAGT CTGGACTGGT TGTGCTGTAA GACTCTGCAG
    TGTGGCGTGA GCTGTGTTAT TCAGGACCTG ACCCTTTGCA CTGACCTCAC TGACTGCCCT
    GAGATACAGT TGCTGAAGGA GACGTTTTTG CTTCTTAAAC CCTCGCTGGA TTTTAGCGAC
    GGCCGCGTGG ATCCCTCGCT GTTAGTGACA GAGATCTTTG CCAGGCTACA GGGTCTTGCT
    GATCATTACC CCTCCATGAT TGGTCAACTG TGTTCTCAAT GTATGGACTG GTTTGAATCC
    TATTCTGACC CATTACTTGT GCCCAAATGC AGCTTCTTTG AAGCCCCTGG TGGCCCACTA
    AAGAGCACCC TCAGAGGATT CACTAAAGGG GTGAAGGCTG TTGTTCTGTG CTCAAAGAGG
    AAGCTGCTGT TAGCAGGTTC AGAGGATGGA CTGATCATAG TCTGGAGTCT AAACAATCTG
    CAGCCTCTTC ACACTCTTTC TGGACATAAA GCCGGGGTGC TGTCCCTGAA GCTGACGGAC
    AAAACCAATA ACTGTTTGTC TACTGGGCTC GATGGGACTC TGAGAAAATG GTGTCTGATA
    AGTGGACTGC AGCTTTTCTG CATCACTGAT GCCGTTTCTG TCCAAACACA CAGCCCTACA
    CACATCCATA TGAATGAAGA AAGGAAGATC ATCATAACCA ACCCAAGCAG CCAAGTTAAA
    GCTTGGGATT TGGACACTGC AGAGCCTCTG TTTGAGCTGA CCGCAGTGAT TGGTTCTGTT
    CTTGGCATTG TGGAAGATGA AATAGCGTGC ATATGCAGTG AGGCGATACT CTGCTTTTAT
    GACATATCCA CCGGCACTCA GACTTCCCAG ACCCTCCTGC CCTACTCTGC CGGACACCAT
    AACATCACCT GCTGTCTCAC ACTCACCAAA CACTCCAAGA TACTCATTGC TTTTGGGGAA
    AGCCGTCTAC ATTCGGTGTG GAGCCATGAA ATGGAGTCTG TGATTGATCT TCCATCTCCA
    GCTCTCTTTC TCAGAACATC TGAGGATGAA AAACTGCTGC TTGCAGGATG TGACAGAACA
    TTGTGCGTGT TCCTCGTGAC ATTGACTTCA ATGCACAAGG TTCTGGATCT TCAACATGAT
    GCATCTATCC TGTGCGCCGT GTCAAACAGC GAAGGTAGCG AAATCATCAC GAGTGCCCAG
    GATCGTATAA TACGGGTATG GAGCGTAACC ACAGGAGCCC TGCTGGACTG GATCGGTGTG
    ATGGATTCCT CTGTGTCCTC ATTGGCTGTA CATCAGCGCT TTATTATTTC CGCCTCTGTT
    ATTGTCAACC AGCTTAAAGT ATGGCAGCTT GACCACAACA CCACGCACAG GAACAAGAGT
    TTCATCCCCG CATACTGCCC CTTGGCAGTC CTGTCGAAAG ATGGTGATAC TGTATTCTAT
    GTCAAGGAGG GAAATAAAAC CGAGGTTTTC ACCTGGAGCT GTTCGGAAGG TGAAACATGT
    CTGCAGTTAG ACAGCATGGA CGTTTCCTCA GAGGTGTGCT GTATGGAATT AGCCCAGCAG
    AAACGGCTCC TCTTCTGCGG TCTGCACACT GGAACCATCT TTATTTACCC GCTTGACTTT
    ATCCCAGAAA CCTTGTGCCT ACCGCCTCCG GAAAGCCTAC CAATGGTACG CAGCATGGCC
    ATCAGCCCAC GAGAGGACAA AATGGCCGTC GCATATGAGG ATGCCGTCTG TCTGTTTGAG
    ATTACAGCGA GGGACAGCTT CCCTTGCGTC GATGGACCAT TTGAAAGGTA CTCGCTTGAC
    CTGTTACATT CCTCAATCTC CGCCATGGCT TTGTTGTCCG ACTGCAGGCT GCTGTATGGG
    ACGTTTGGCG GCGAAGTGGT GATCTACGAC TTTAAAAGCG CCACAGCAGC AGAGCTGGAT
    CACCACGGTG CAGCCATAAC CTGCATCACC ATGAGCAACT GGGATGCGCA GGCACTGATT
    GGCTCTCAGG ACTGTGTTCA GAAGCTGTGG AATTTGAGTC CCGTCTTACT GAATCACACC
    ATGGAGTACA AGGGCTTTTA TTTTGAAGGA GTTCTATGTG CTGTCTTCTC AATAAATGAT
    AAATACGTGT TCACTGGATC CTTGGACAAA ACCGTCAAAG TCTGGGATGT TTCGAGTGGT
    TGTTTGCTGT ATATCCAGTA CGTCTACTCT CCGGTCATAA GGATGATGCC ACACAAGGAT
    GGATTTGTAG CTGTATCCCA GCATGGCTCA TTCATCAAGG AAGGTTTTCG CTGTCCAGAC
    AGCCTCAAGC CTGGATATAA CCCCCTCCGG AACTTCCAAG TACATTACAA AGTCACTTCA
    CGAGACAAAA GTCTGAACGT TCCACACAGC GTGATCAATG AGCTGCCAGA TTACAACCCT
    GCTCAGTTCA ACTTCATTGG CATCGGTCTG ATCAAATCCA AACCATCCAA TACGTGCGTT
    CTCCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49402
    Clone ID Related Accession (Same CDS sequence) XM_009305820.2 , XM_009305820.3
    Accession Version XM_009305820.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT domain- and WD repeat-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305820.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGATGTGG TGGAGATGAG AGAGGCACTT GGGTTGGAGG TTCTGCGGGG ACAAAGCGCT 
    GATTCTCTGC AGCCTACTTC CAGAATGGTG CGGGTGTTCA TCAGCTCCAC TCACTCAGAC
    ATGAAAAATG AGAGAAATGC ATTCTGGGAG AAGACCTATT CGGAGCTACA GACCCACTGC
    CAAAGTTTGG GTCTTGTTTT TGAGGTAGTG GATTTCAAAT GTGGAATGCG TGACATCACT
    GCATCTGATC ATTTAATAAC TGAACTGTGC ATTAAAGAAA TCGAAACCTG CCAGAGGATA
    TCAGATGGGC CAACTTTTAT AGCTTTGATC GGTAATCAGT ATGGCCATCG CCTTGTCCCG
    CGCCTCATAC CAGAGGCAGA GTTTGAGTTG CTGCTATCAA AACTTTCCAA AGATCCAGAG
    AGTTTAAAGC TCCTTAACCA ATGGTTTTAC AAGGACAGTA ACTCAATCCC ACCCACATAT
    ATCCTCCTGC CAATCACGGT GCACTACCCA CATTACAATG AGAGAGATTC TGAGAACGCA
    CAGTTGCGGG ATAATGATGT CATATCCTGG AATCTAACAG AGTCACGGCT GCTGCAGGTT
    CTCCGCAGTG CAGCTCTACA GGCTGAGAGA GACGGGGATT TCACTGCTGA CAGCAAGCAC
    AAGTTTTTCA AATCTGTTTT AGAATATGAG ATGGAGCATG GCTTTCTCAG GACTCAAAGG
    GATGCATCGG CTGTGCTGTT TTTCCGCGAA CTGCCTCGAC TTAGTAAAAG AGACTGCAAG
    AAAAACTTTG CAAAGTTTCT GCTGGACGTC ACAGCCGACG GCTTGCTGGA CACACAAGCC
    CGGGAGCTCT TGACTGACCT GAAGCAACGC ATCTGCAACA CCGGTCGCCT CCAACCACTC
    TGTATGGAGC TCAGCAAAGG AGCCATGGAC CCAAGCCGCA AAGAACACAA GGAATACCTG
    GGTAAGCTGT GTGAACAGGT AACATCTCAA TTGAAGGAAC TGATCAGCAG AAATGCTCTG
    CAAACCTCAG GAACTTCAGT AGATCCAGTA TGGTCCTGGC TAAAACAGGA GATCTTTCAT
    CATGCCAAGT TGAGTGAGGA GAAGTGTGCT GTGTTTAAAG GGAGAGACGA CATTCTGGGA
    AAGATCTCCA TCACCCTGTG GGAATCCAAT GATGTTTCTC ATGGACCTCT TCTAGTCCAT
    GGACCTCCAG GAGTTGGTAA AACAGCTCTG CTTTGCAAAC TGGCCAAGGA AATGCGAGCA
    GTTGTTGACA ATCGGGGAGT TGTTCTGCTG AGGTTGCTGG GAACATCTCT GCTCAGTTCA
    GATGTCGATT CTGTTCTTCG AGGTGTTTGT CTTCAAGTGT GTGGAGCTCT AGGGTTGCAG
    TCCCCATACT CGCATATAGC GTACACGCAT GAAGGGCTAG TTCGATTTTT CCATTCCATG
    CTGGAAGACG TGTCAAAACA AGGAGACACT CTTCTACTCA TTCTAGACTC ATTGGAGCAG
    CTTTTAGAGA CAAACAAACT TCATAAACTA GACTGGATAC CTAAAATAAT TCCACCCAAT
    GTGCAAATCG TATTCTCAAC TTCTAATGAG TGCTTGGACA AATGTAAATC TTTGGTTTCA
    GCGGAAGAAA ACATCTTTAA ATTGGAACTT CTCACGAAAG ATCAAGGGAG AGACGTCATT
    GATGCATATA TGAGTGCAGC TGAACGCAAG CTGACGGCAG AACAGCAAGA CGCAATCTTA
    CGCTACTTTG AGCAAAGTGG AAACCCTTTG CATCTGAAAC TCATGCTAGA CACTGCCAAA
    CTGTGGTCCT CCTACACCCC CATGTCTAGT GTTCACCTGG ACAAGTCGGC GGAGGAAGTG
    ATGACACAGT TTGTCGAGTC ACTTGAGGTG AATCATGGGA AGAAGCTAGT CAGTCATGCT
    CTGAGCTTCA TCCTGTTATC AAGATATGGA CTTTCAGAGG CAGAGTTACA GGATGTCCTA
    TCATTAGATA ATGATGTTCT AGCTGAGATC TATAAATGCT GGCTTCCTCC GAGCCATACG
    CTCATGCGCT TCCCACGTTT TCATTGGTCA AGACTCAGGC ATGACCTGAG TGCTCATCTG
    ACGGAGAGAT GGGAAGCCGG CGTTCTTCTG CTGGGTTTCA CTCACAGGCA GTTTGCAGAA
    CTGGTAAGGA GGAGATATTT GTCACGTGAT GTGAAAGCTG ACATGCACAC GATCCTCGCC
    GAGTATTTCT CCGGACAGTG GAGTAAGGGG CAGCTCAGAC CCATCCTTCT CCCCTCACTG
    AGCGCATTTC TCAATGCTGA CAGAAAGGTA TCACCCCAGC CCTTGTGGTT TTCTGAAGAA
    CTTGCCAATT TGCGTAAGAT TCATGAACTT CCTTATCACC TTGCTCACGC AGGAAAATGG
    GACGAGCTAC GACAATCAGT GCTTGGGAGT CTGGACTGGT TGTGCTGTAA GACTCTGCAG
    TGTGGCGTGA GCTGTGTTAT TCAGGACCTG ACCCTTTGCA CTGACCTCAC TGACTGCCCT
    GAGATACAGT TGCTGAAGGA GACGTTTTTG CTTCTTAAAC CCTCGCTGGA TTTTAGCGAC
    GGCCGCGTGG ATCCCTCGCT GTTAGTGACA GAGATCTTTG CCAGGCTACA GGGTCTTGCT
    GATCATTACC CCTCCATGAT TGGTCAACTG TGTTCTCAAT GTATGGACTG GTTTGAATCC
    TATTCTGACC CATTACTTGT GCCCAAATGC AGCTTCTTTG AAGCCCCTGG TGGCCCACTA
    AAGAGCACCC TCAGAGGATT CACTAAAGGG GTGAAGGCTG TTGTTCTGTG CTCAAAGAGG
    AAGCTGCTGT TAGCAGGTTC AGAGGATGGA CTGATCATAG TCTGGAGTCT AAACAATCTG
    CAGCCTCTTC ACACTCTTTC TGGACATAAA GCCGGGGTGC TGTCCCTGAA GCTGACGGAC
    AAAACCAATA ACTGTTTGTC TACTGGGCTC GATGGGACTC TGAGAAAATG GTGTCTGATA
    AGTGGACTGC AGCTTTTCTG CATCACTGAT GCCGTTTCTG TCCAAACACA CAGCCCTACA
    CACATCCATA TGAATGAAGA AAGGAAGATC ATCATAACCA ACCCAAGCAG CCAAGTTAAA
    GCTTGGGATT TGGACACTGC AGAGCCTCTG TTTGAGCTGA CCGCAGTGAT TGGTTCTGTT
    CTTGGCATTG TGGAAGATGA AATAGCGTGC ATATGCAGTG AGGCGATACT CTGCTTTTAT
    GACATATCCA CCGGCACTCA GACTTCCCAG ACCCTCCTGC CCTACTCTGC CGGACACCAT
    AACATCACCT GCTGTCTCAC ACTCACCAAA CACTCCAAGA TACTCATTGC TTTTGGGGAA
    AGCCGTCTAC ATTCGGTGTG GAGCCATGAA ATGGAGTCTG TGATTGATCT TCCATCTCCA
    GCTCTCTTTC TCAGAACATC TGAGGATGAA AAACTGCTGC TTGCAGGATG TGACAGAACA
    TTGTGCGTGT TCCTCGTGAC ATTGACTTCA ATGCACAAGG TTCTGGATCT TCAACATGAT
    GCATCTATCC TGTGCGCCGT GTCAAACAGC GAAGGTAGCG AAATCATCAC GAGTGCCCAG
    GATCGTATAA TACGGGTATG GAGCGTAACC ACAGGAGCCC TGCTGGACTG GATCGGTGTG
    ATGGATTCCT CTGTGTCCTC ATTGGCTGTA CATCAGCGCT TTATTATTTC CGCCTCTGTT
    ATTGTCAACC AGCTTAAAGT ATGGCAGCTT GACCACAACA CCACGCACAG GAACAAGAGT
    TTCATCCCCG CATACTGCCC CTTGGCAGTC CTGTCGAAAG ATGGTGATAC TGTATTCTAT
    GTCAAGGAGG GAAATAAAAC CGAGGTTTTC ACCTGGAGCT GTTCGGAAGG TGAAACATGT
    CTGCAGTTAG ACAGCATGGA CGTTTCCTCA GAGGTGTGCT GTATGGAATT AGCCCAGCAG
    AAACGGCTCC TCTTCTGCGG TCTGCACACT GGAACCATCT TTATTTACCC GCTTGACTTT
    ATCCCAGAAA CCTTGTGCCT ACCGCCTCCG GAAAGCCTAC CAATGGTACG CAGCATGGCC
    ATCAGCCCAC GAGAGGACAA AATGGCCGTC GCATATGAGG ATGCCGTCTG TCTGTTTGAG
    ATTACAGCGA GGGACAGCTT CCCTTGCGTC GATGGACCAT TTGAAAGGTA CTCGCTTGAC
    CTGTTACATT CCTCAATCTC CGCCATGGCT TTGTTGTCCG ACTGCAGGCT GCTGTATGGG
    ACGTTTGGCG GCGAAGTGGT GATCTACGAC TTTAAAAGCG CCACAGCAGC AGAGCTGGAT
    CACCACGGTG CAGCCATAAC CTGCATCACC ATGAGCAACT GGGATGCGCA GGCACTGATT
    GGCTCTCAGG ACTGTGTTCA GAAGCTGTGG AATTTGAGTC CCGTCTTACT GAATCACACC
    ATGGAGTACA AGGGCTTTTA TTTTGAAGGA GTTCTATGTG CTGTCTTCTC AATAAATGAT
    AAATACGTGT TCACTGGATC CTTGGACAAA ACCGTCAAAG TCTGGGATGT TTCGAGTGGT
    TGTTTGCTGT ATATCCAGTA CGTCTACTCT CCGGTCATAA GGATGATGCC ACACAAGGAT
    GGATTTGTAG CTGTATCCCA GCATGGCTCA TTCATCAAGG AAGGTTTTCG CTGTCCAGAC
    AGCCTCAAGC CTGGATATAA CCCCCTCCGG AACTTCCAAG TACATTACAA AGTCACTTCA
    CGAGACAAAA GTCTGAACGT TCCACACAGC GTGATCAATG AGCTGCCAGA TTACAACCCT
    GCTCAGTTCA ACTTCATTGG CATCGGTCTG ATCAAATCCA AACCATCCAA TACGTGCGTT
    CTCCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304095.1
    CDS521..5389
    Translation

    Target ORF information:

    RefSeq Version XM_009305820.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT and WD repeat domain containing 1 (nwd1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305820.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGATGTGG TGGAGATGAG AGAGGCACTT GGGTTGGAGG TTCTGCGGGG ACAAAGCGCT 
    GATTCTCTGC AGCCTACTTC CAGAATGGTG CGGGTGTTCA TCAGCTCCAC TCACTCAGAC
    ATGAAAAATG AGAGAAATGC ATTCTGGGAG AAGACCTATT CGGAGCTACA GACCCACTGC
    CAAAGTTTGG GTCTTGTTTT TGAGGTAGTG GATTTCAAAT GTGGAATGCG TGACATCACT
    GCATCTGATC ATTTAATAAC TGAACTGTGC ATTAAAGAAA TCGAAACCTG CCAGAGGATA
    TCAGATGGGC CAACTTTTAT AGCTTTGATC GGTAATCAGT ATGGCCATCG CCTTGTCCCG
    CGCCTCATAC CAGAGGCAGA GTTTGAGTTG CTGCTATCAA AACTTTCCAA AGATCCAGAG
    AGTTTAAAGC TCCTTAACCA ATGGTTTTAC AAGGACAGTA ACTCAATCCC ACCCACATAT
    ATCCTCCTGC CAATCACGGT GCACTACCCA CATTACAATG AGAGAGATTC TGAGAACGCA
    CAGTTGCGGG ATAATGATGT CATATCCTGG AATCTAACAG AGTCACGGCT GCTGCAGGTT
    CTCCGCAGTG CAGCTCTACA GGCTGAGAGA GACGGGGATT TCACTGCTGA CAGCAAGCAC
    AAGTTTTTCA AATCTGTTTT AGAATATGAG ATGGAGCATG GCTTTCTCAG GACTCAAAGG
    GATGCATCGG CTGTGCTGTT TTTCCGCGAA CTGCCTCGAC TTAGTAAAAG AGACTGCAAG
    AAAAACTTTG CAAAGTTTCT GCTGGACGTC ACAGCCGACG GCTTGCTGGA CACACAAGCC
    CGGGAGCTCT TGACTGACCT GAAGCAACGC ATCTGCAACA CCGGTCGCCT CCAACCACTC
    TGTATGGAGC TCAGCAAAGG AGCCATGGAC CCAAGCCGCA AAGAACACAA GGAATACCTG
    GGTAAGCTGT GTGAACAGGT AACATCTCAA TTGAAGGAAC TGATCAGCAG AAATGCTCTG
    CAAACCTCAG GAACTTCAGT AGATCCAGTA TGGTCCTGGC TAAAACAGGA GATCTTTCAT
    CATGCCAAGT TGAGTGAGGA GAAGTGTGCT GTGTTTAAAG GGAGAGACGA CATTCTGGGA
    AAGATCTCCA TCACCCTGTG GGAATCCAAT GATGTTTCTC ATGGACCTCT TCTAGTCCAT
    GGACCTCCAG GAGTTGGTAA AACAGCTCTG CTTTGCAAAC TGGCCAAGGA AATGCGAGCA
    GTTGTTGACA ATCGGGGAGT TGTTCTGCTG AGGTTGCTGG GAACATCTCT GCTCAGTTCA
    GATGTCGATT CTGTTCTTCG AGGTGTTTGT CTTCAAGTGT GTGGAGCTCT AGGGTTGCAG
    TCCCCATACT CGCATATAGC GTACACGCAT GAAGGGCTAG TTCGATTTTT CCATTCCATG
    CTGGAAGACG TGTCAAAACA AGGAGACACT CTTCTACTCA TTCTAGACTC ATTGGAGCAG
    CTTTTAGAGA CAAACAAACT TCATAAACTA GACTGGATAC CTAAAATAAT TCCACCCAAT
    GTGCAAATCG TATTCTCAAC TTCTAATGAG TGCTTGGACA AATGTAAATC TTTGGTTTCA
    GCGGAAGAAA ACATCTTTAA ATTGGAACTT CTCACGAAAG ATCAAGGGAG AGACGTCATT
    GATGCATATA TGAGTGCAGC TGAACGCAAG CTGACGGCAG AACAGCAAGA CGCAATCTTA
    CGCTACTTTG AGCAAAGTGG AAACCCTTTG CATCTGAAAC TCATGCTAGA CACTGCCAAA
    CTGTGGTCCT CCTACACCCC CATGTCTAGT GTTCACCTGG ACAAGTCGGC GGAGGAAGTG
    ATGACACAGT TTGTCGAGTC ACTTGAGGTG AATCATGGGA AGAAGCTAGT CAGTCATGCT
    CTGAGCTTCA TCCTGTTATC AAGATATGGA CTTTCAGAGG CAGAGTTACA GGATGTCCTA
    TCATTAGATA ATGATGTTCT AGCTGAGATC TATAAATGCT GGCTTCCTCC GAGCCATACG
    CTCATGCGCT TCCCACGTTT TCATTGGTCA AGACTCAGGC ATGACCTGAG TGCTCATCTG
    ACGGAGAGAT GGGAAGCCGG CGTTCTTCTG CTGGGTTTCA CTCACAGGCA GTTTGCAGAA
    CTGGTAAGGA GGAGATATTT GTCACGTGAT GTGAAAGCTG ACATGCACAC GATCCTCGCC
    GAGTATTTCT CCGGACAGTG GAGTAAGGGG CAGCTCAGAC CCATCCTTCT CCCCTCACTG
    AGCGCATTTC TCAATGCTGA CAGAAAGGTA TCACCCCAGC CCTTGTGGTT TTCTGAAGAA
    CTTGCCAATT TGCGTAAGAT TCATGAACTT CCTTATCACC TTGCTCACGC AGGAAAATGG
    GACGAGCTAC GACAATCAGT GCTTGGGAGT CTGGACTGGT TGTGCTGTAA GACTCTGCAG
    TGTGGCGTGA GCTGTGTTAT TCAGGACCTG ACCCTTTGCA CTGACCTCAC TGACTGCCCT
    GAGATACAGT TGCTGAAGGA GACGTTTTTG CTTCTTAAAC CCTCGCTGGA TTTTAGCGAC
    GGCCGCGTGG ATCCCTCGCT GTTAGTGACA GAGATCTTTG CCAGGCTACA GGGTCTTGCT
    GATCATTACC CCTCCATGAT TGGTCAACTG TGTTCTCAAT GTATGGACTG GTTTGAATCC
    TATTCTGACC CATTACTTGT GCCCAAATGC AGCTTCTTTG AAGCCCCTGG TGGCCCACTA
    AAGAGCACCC TCAGAGGATT CACTAAAGGG GTGAAGGCTG TTGTTCTGTG CTCAAAGAGG
    AAGCTGCTGT TAGCAGGTTC AGAGGATGGA CTGATCATAG TCTGGAGTCT AAACAATCTG
    CAGCCTCTTC ACACTCTTTC TGGACATAAA GCCGGGGTGC TGTCCCTGAA GCTGACGGAC
    AAAACCAATA ACTGTTTGTC TACTGGGCTC GATGGGACTC TGAGAAAATG GTGTCTGATA
    AGTGGACTGC AGCTTTTCTG CATCACTGAT GCCGTTTCTG TCCAAACACA CAGCCCTACA
    CACATCCATA TGAATGAAGA AAGGAAGATC ATCATAACCA ACCCAAGCAG CCAAGTTAAA
    GCTTGGGATT TGGACACTGC AGAGCCTCTG TTTGAGCTGA CCGCAGTGAT TGGTTCTGTT
    CTTGGCATTG TGGAAGATGA AATAGCGTGC ATATGCAGTG AGGCGATACT CTGCTTTTAT
    GACATATCCA CCGGCACTCA GACTTCCCAG ACCCTCCTGC CCTACTCTGC CGGACACCAT
    AACATCACCT GCTGTCTCAC ACTCACCAAA CACTCCAAGA TACTCATTGC TTTTGGGGAA
    AGCCGTCTAC ATTCGGTGTG GAGCCATGAA ATGGAGTCTG TGATTGATCT TCCATCTCCA
    GCTCTCTTTC TCAGAACATC TGAGGATGAA AAACTGCTGC TTGCAGGATG TGACAGAACA
    TTGTGCGTGT TCCTCGTGAC ATTGACTTCA ATGCACAAGG TTCTGGATCT TCAACATGAT
    GCATCTATCC TGTGCGCCGT GTCAAACAGC GAAGGTAGCG AAATCATCAC GAGTGCCCAG
    GATCGTATAA TACGGGTATG GAGCGTAACC ACAGGAGCCC TGCTGGACTG GATCGGTGTG
    ATGGATTCCT CTGTGTCCTC ATTGGCTGTA CATCAGCGCT TTATTATTTC CGCCTCTGTT
    ATTGTCAACC AGCTTAAAGT ATGGCAGCTT GACCACAACA CCACGCACAG GAACAAGAGT
    TTCATCCCCG CATACTGCCC CTTGGCAGTC CTGTCGAAAG ATGGTGATAC TGTATTCTAT
    GTCAAGGAGG GAAATAAAAC CGAGGTTTTC ACCTGGAGCT GTTCGGAAGG TGAAACATGT
    CTGCAGTTAG ACAGCATGGA CGTTTCCTCA GAGGTGTGCT GTATGGAATT AGCCCAGCAG
    AAACGGCTCC TCTTCTGCGG TCTGCACACT GGAACCATCT TTATTTACCC GCTTGACTTT
    ATCCCAGAAA CCTTGTGCCT ACCGCCTCCG GAAAGCCTAC CAATGGTACG CAGCATGGCC
    ATCAGCCCAC GAGAGGACAA AATGGCCGTC GCATATGAGG ATGCCGTCTG TCTGTTTGAG
    ATTACAGCGA GGGACAGCTT CCCTTGCGTC GATGGACCAT TTGAAAGGTA CTCGCTTGAC
    CTGTTACATT CCTCAATCTC CGCCATGGCT TTGTTGTCCG ACTGCAGGCT GCTGTATGGG
    ACGTTTGGCG GCGAAGTGGT GATCTACGAC TTTAAAAGCG CCACAGCAGC AGAGCTGGAT
    CACCACGGTG CAGCCATAAC CTGCATCACC ATGAGCAACT GGGATGCGCA GGCACTGATT
    GGCTCTCAGG ACTGTGTTCA GAAGCTGTGG AATTTGAGTC CCGTCTTACT GAATCACACC
    ATGGAGTACA AGGGCTTTTA TTTTGAAGGA GTTCTATGTG CTGTCTTCTC AATAAATGAT
    AAATACGTGT TCACTGGATC CTTGGACAAA ACCGTCAAAG TCTGGGATGT TTCGAGTGGT
    TGTTTGCTGT ATATCCAGTA CGTCTACTCT CCGGTCATAA GGATGATGCC ACACAAGGAT
    GGATTTGTAG CTGTATCCCA GCATGGCTCA TTCATCAAGG AAGGTTTTCG CTGTCCAGAC
    AGCCTCAAGC CTGGATATAA CCCCCTCCGG AACTTCCAAG TACATTACAA AGTCACTTCA
    CGAGACAAAA GTCTGAACGT TCCACACAGC GTGATCAATG AGCTGCCAGA TTACAACCCT
    GCTCAGTTCA ACTTCATTGG CATCGGTCTG ATCAAATCCA AACCATCCAA TACGTGCGTT
    CTCCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.