NWD1 cDNA ORF clone, Bos taurus(cattle)

The following NWD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NWD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa236144 XM_024994420.1
Latest version!
Bos taurus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236142 XM_015471888.2
Latest version!
Bos taurus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236143 XM_024994419.1
Latest version!
Bos taurus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa91036 XM_015471888.1
Latest version!
Bos taurus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa38635 NM_001102210.1
Latest version!
Bos taurus NACHT and WD repeat domain containing 1 (NWD1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa236144
    Clone ID Related Accession (Same CDS sequence) XM_024994420.1
    Accession Version XM_024994420.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4329bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT domain- and WD repeat-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACGCAG AGAGGGAAGC CCTCCAGAGC ACCGCCTACC CCGAAGTGCA GACCTTCTGC 
    CAGAAGCACG GCCTGATGTT CGAGGTGGTT GACCTGAGGT GGGGTATTCG GAACACTGAG
    GCCACTAACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TTGACCGGTG TCGGAAAACA
    TCCATAGGGC CAGCTTTTGT TGCCCTCGTG GGTGACCAGT ACGGCCCCTG CCCCGTCCCC
    ACGCAGATCG AGGAAAAGGA GTGGGAGGCG CTGAAGGCTC AGCTGACCGC CAGGCCCCGC
    GACCTGGAGC TGGTGGCACG ATGCTTCTGG AGAGACGAGA ACGCGGTGCC CCCCGCCTAC
    CTGCTGCAGG CCCTGGGCAC TGGGGAGGCC TCTGGGCCTG AAGAGGCCAC CCTGCTCTCT
    GCACTGCGCT CCGGAGCCCA GGAGGCCCGC AGGCTGGGGC TCATCAGCCA GGAGCGGTGG
    TACTGCTACC ACCGGTCAGT CATTGAGTGG GAAATAGAAC GGGCCTTGCT GAGCTCAACG
    GTTGGGGATC ACGGGGCAAC AGTCTTCCTG AGAGAGATCC AAGACCTCAA TAAACACATC
    CTAGAAGACT GTGCCCTCAG AATGGTGGAC CGTCTTGCAG ATGGTTGCCT GGACACGGAT
    GCCCAGAACC TTCTCAGCAA CCTCAAGGGG CGCATCGCTG ACCGGCAGCC TGGAGTTCTC
    AAGGCCCACC ACCTAGCGTG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCC
    CGATACCTGA AGGAGCTGGG GGAGCAGTTT GTGGCCAGGG CCAACCATCA GGTTCTTGAG
    TGCCTCCGGG AGCTGGAGGC AGGCAGGCAG GAGTTGGCAT GGCTCTACCA GGAGATCCGC
    CACCACCTGG GACTCAGTGC CGAGGTCAAC CGGACCTTCT GTGGGCGCCA GGAGCTCCTG
    ACCCAGCTGG GCCAGCGACT GCGACAGAAT GATGGCCACC CCCACCCACC CCTGGTGCTT
    TTTGGACCCC CAGGGATTGG AAAGACGGCT CTGATGTGCA AGCTGGCTGA GCAAATGTCG
    GGGCTGCTCG GCCGCAAGAC AGTGACCATT CTGCGGCTTC TGGGGACGTC ACAGATGAGC
    TCTGAGGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG
    CCCCTGCCCC CTGCCCAGGT CCTAGAGGCC CACACCAGGC TGGTCCAGTT TTTCCACATC
    CTCCTCCACA CCATCTCCAG CCGAAACTTT GAGTCCCTCG TGATCCTGCT GGATTCGGTG
    GATGAGGTGG ACTCTGTCCA CTGTGCTAGG AGGATCCCCT GGCTGCCTCC CAAATGTCCT
    CCGAGGGTCC ACCTCATCGT CTCGGCCTGC TCTGGACAGC GGGGGGTTTT AGACACCATT
    CAGCAGGTGC TCACGGACCG AGAGGCTTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA
    GGGCAAGAGA TGATCCAGCT CCTGCTGTCT GCCTCGAGGA GGACACTGAG TCAGGTGCAG
    AGGGACATGC TCTGGGCCAG CCTCCCAGAG TGTGGACACC CAGGGCGGCT GAGGCTGGCT
    TTCGAGGAGG CCCGGAAGTG GGCCTCCTTC ACTGTGCCAG CCCCGCTGGC CTCCACTGCT
    AAGGAGGCGA TGCAACAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCG GCTCCTGGTG
    GCCCGCGTGC TGGGCTACAT TGTGTCTTCC CGGCAGGGGC TCTCAGAGGC GGAGTTAAAG
    GATGTTCTGT CCTTGGATGA TGAGGTCCTG CAGAGCGTGT ACCAAGACTG GACCCCACCC
    AGCAAAGAGC TTCTGCGCTT CCCACCCCTG CTGTGGGTGA GGCTTCGTCG AGATCTGGGT
    AACTGCTTGG CCAGGCGGCC CGTGGACGGC TTCACCCTTC TGGCCATTGC TCACAGACAG
    CTGACCGAGG TGGTCAGAGA GCTCTACCTG TCTGGGCCTG AGAAAGCCAA GAGTCACGGA
    GTCTTGGCCG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA TCAAGAAACT CATCACCCTG
    CCACTCGTGG GAAAACCCCT GAACCTGGAC CGCAAGGTGG CCCCTCAGCC CCTGTGGTTC
    TCCGACATGG TTGCAAACCT GAGGAAGCTG AAGGAGTTAC CCTACCACTT GCTCCATGCC
    GGCCGTATCG AGGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGACCTGGAT TTGCTGCCGG
    GGCCTCTCTG GGGGCATCGA GGGCCTGCTG GAAGACTTTG ACCTGTGCGC CCCATACATG
    GACTGCCCTG AGGTCAGCCT GGTCCGGGAA GCTCTCCAGC TGTGCCGCCC GGCAGTGGAG
    CTCCGAGGCA TGGAGAGAAG CATCCTGTAC ACAGAACTCC TGGCCAGACT CCATTTCTTC
    AGCACTTCAC ATCCCGGGCT GGTGGGACAG CTGTGCCAAC AGGCACAGAG CTGGTTCCGG
    ATGTGCCCAC AGCCTGTGCT GGTGCCCCTT GGAGGATTCC TCCAGCCCCC TGGAGGGCCT
    CTGCGGGCAA CCCTCAGCGG CTGTCACAAA GGCATCACTG CCATGGCATG GAGCCTGGAG
    GAGAAGCTGC TGCTGGTTGG CACGCAGGAG GGCATGGTGG CTGTGTGGGA CATGGAGGAA
    CAGCAGGTGA TCCACATCCT TCCTGGACAC ACAGGAGAGG TAAGGTGTGT GGAAGTGTTT
    GCCCAAGGGA CACATGCTGT CTCTGCCTCG AAAGACCAGA CCTTGCGCTT GTGGGACTTA
    CTGTCTGGCC AGGAGAAATT CACCATTTGG GATGGAGGTT CAAAAGATCC CACTGAATCT
    CAGACCTGGA GCCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTCAGC TTCAGGCTCA
    AAGATCAGTG CTTGGAACCT GGAAACTGCA GAGCTGATTT TCCGTATCCT GGGGGATGCC
    TCTGACCCCT GGGTATGCAT GGCAGTGCTG GCTTCCCAGG CCACACTGCT GACTGTGTCC
    CAGGGTGGCG TGATCAGTCT GTGGAGCTCA GCCACGGGAA CACTGCACAG GAGGCAACGT
    TTGTCCAGCG TCAAAGAAGA TACACTTGCC TGTGGGGTCT CAGTCCAGAA ACGAGGAATG
    ATGGTTACTG GGTCCAGCAC GGGCTCCATC TCTTTGGTTT CCTCCGAAGG AGACAGCCTG
    CTGGAAAAGC TTCCGGAAGC TGTGGGGTTC CTGGTGGTCT CTGAAGACGA GTCTCTTCTC
    GCTGCAGGTT TTGGAAGGTC CGTGAGGATA TTTTTGGCAG ATGCACAGGG ATTTCATCGA
    TTCCTGGCCA CTGACCTTGA ACATGAAGAT GTGGTAGAGA CAGCCGTTTT TGGTCCTGAA
    AACAATCTGA TTGTCACCGG GTCCCGGGAT GCACTCATTC AGGTGTGGAG TCTGTCAGCG
    CAGGGGACTC TGCTGGACAT CTTGGACGGT GTCGGGGCCC CTGTGAGCCT GCTGGCCCGG
    GGTGGGGCCT TGGTGGCATC TGCTTCCCGT CAGTCCTCAT CTTTTAAAGT CTGGGATCTC
    ACCTGTGCTC AGAAGCCGAG GGCCTCTAAC CCATTCTTGG ACCGCACTGG CCTCACAGCA
    GTGTCACACA ACGGCAGCTA CGTCTATTTC CCTAAAACTG GGGACAAAAA CAAAGTCAGC
    ATTTGGGACT TGGCAGAAGG TGAGGAGCAA GATGCTCTGG ACACCTCCAA CGAGGTCAGG
    TGTCTGGAGG TTGCCGAGCA GGCCAATCTC CTTTTCACTG GCCTCGTTTC GGGGATTGTC
    CTGGTGTTCC CCCTGAACTC CAGACAAGAC GTGATGTGTA TCCCTCCTCC AGAAGCCCGG
    AAAGCCGTCA ACTGCATGGC TCTTAGTAAG GACGAGGGGC ACCTGGCCAT TGCCTATGAC
    AGCACCGTCT TGGTGCTGGA CATCAGCCCG GGGGACCCCT GTCCAGTCAT CGACGGGCCA
    ATATACACCT TCTATACCCA ACTGCCTGAC ACCATAGCCA GTGTGGCCAT TCTGGCTGAC
    TGTCGTGTCA TCTACGGCAT GATCAACGGT GACCTCTTCC TTTACGAGTG TGTGAATTCC
    AAGGTGTTCC CTCTGGAGGC CCATGGGAGC CGAGTCACCT GTGTGGAGGT CAGCCACAAA
    GAGCAGCTGG CAGTCAGCGG GGCCGAGGAA GCCCTACTGT GTCTCTGGGA CCTGCAGGTG
    TGCAAGTGGA AATTCGAGAT GAGCTACACG GCACACTGCT GGCTGTCCAG TTTGTGCATG
    CCGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850188.1
    CDS551..4879
    Translation

    Target ORF information:

    RefSeq Version XM_024994420.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994420.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACGCAG AGAGGGAAGC CCTCCAGAGC ACCGCCTACC CCGAAGTGCA GACCTTCTGC 
    CAGAAGCACG GCCTGATGTT CGAGGTGGTT GACCTGAGGT GGGGTATTCG GAACACTGAG
    GCCACTAACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TTGACCGGTG TCGGAAAACA
    TCCATAGGGC CAGCTTTTGT TGCCCTCGTG GGTGACCAGT ACGGCCCCTG CCCCGTCCCC
    ACGCAGATCG AGGAAAAGGA GTGGGAGGCG CTGAAGGCTC AGCTGACCGC CAGGCCCCGC
    GACCTGGAGC TGGTGGCACG ATGCTTCTGG AGAGACGAGA ACGCGGTGCC CCCCGCCTAC
    CTGCTGCAGG CCCTGGGCAC TGGGGAGGCC TCTGGGCCTG AAGAGGCCAC CCTGCTCTCT
    GCACTGCGCT CCGGAGCCCA GGAGGCCCGC AGGCTGGGGC TCATCAGCCA GGAGCGGTGG
    TACTGCTACC ACCGGTCAGT CATTGAGTGG GAAATAGAAC GGGCCTTGCT GAGCTCAACG
    GTTGGGGATC ACGGGGCAAC AGTCTTCCTG AGAGAGATCC AAGACCTCAA TAAACACATC
    CTAGAAGACT GTGCCCTCAG AATGGTGGAC CGTCTTGCAG ATGGTTGCCT GGACACGGAT
    GCCCAGAACC TTCTCAGCAA CCTCAAGGGG CGCATCGCTG ACCGGCAGCC TGGAGTTCTC
    AAGGCCCACC ACCTAGCGTG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCC
    CGATACCTGA AGGAGCTGGG GGAGCAGTTT GTGGCCAGGG CCAACCATCA GGTTCTTGAG
    TGCCTCCGGG AGCTGGAGGC AGGCAGGCAG GAGTTGGCAT GGCTCTACCA GGAGATCCGC
    CACCACCTGG GACTCAGTGC CGAGGTCAAC CGGACCTTCT GTGGGCGCCA GGAGCTCCTG
    ACCCAGCTGG GCCAGCGACT GCGACAGAAT GATGGCCACC CCCACCCACC CCTGGTGCTT
    TTTGGACCCC CAGGGATTGG AAAGACGGCT CTGATGTGCA AGCTGGCTGA GCAAATGTCG
    GGGCTGCTCG GCCGCAAGAC AGTGACCATT CTGCGGCTTC TGGGGACGTC ACAGATGAGC
    TCTGAGGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG
    CCCCTGCCCC CTGCCCAGGT CCTAGAGGCC CACACCAGGC TGGTCCAGTT TTTCCACATC
    CTCCTCCACA CCATCTCCAG CCGAAACTTT GAGTCCCTCG TGATCCTGCT GGATTCGGTG
    GATGAGGTGG ACTCTGTCCA CTGTGCTAGG AGGATCCCCT GGCTGCCTCC CAAATGTCCT
    CCGAGGGTCC ACCTCATCGT CTCGGCCTGC TCTGGACAGC GGGGGGTTTT AGACACCATT
    CAGCAGGTGC TCACGGACCG AGAGGCTTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA
    GGGCAAGAGA TGATCCAGCT CCTGCTGTCT GCCTCGAGGA GGACACTGAG TCAGGTGCAG
    AGGGACATGC TCTGGGCCAG CCTCCCAGAG TGTGGACACC CAGGGCGGCT GAGGCTGGCT
    TTCGAGGAGG CCCGGAAGTG GGCCTCCTTC ACTGTGCCAG CCCCGCTGGC CTCCACTGCT
    AAGGAGGCGA TGCAACAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCG GCTCCTGGTG
    GCCCGCGTGC TGGGCTACAT TGTGTCTTCC CGGCAGGGGC TCTCAGAGGC GGAGTTAAAG
    GATGTTCTGT CCTTGGATGA TGAGGTCCTG CAGAGCGTGT ACCAAGACTG GACCCCACCC
    AGCAAAGAGC TTCTGCGCTT CCCACCCCTG CTGTGGGTGA GGCTTCGTCG AGATCTGGGT
    AACTGCTTGG CCAGGCGGCC CGTGGACGGC TTCACCCTTC TGGCCATTGC TCACAGACAG
    CTGACCGAGG TGGTCAGAGA GCTCTACCTG TCTGGGCCTG AGAAAGCCAA GAGTCACGGA
    GTCTTGGCCG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA TCAAGAAACT CATCACCCTG
    CCACTCGTGG GAAAACCCCT GAACCTGGAC CGCAAGGTGG CCCCTCAGCC CCTGTGGTTC
    TCCGACATGG TTGCAAACCT GAGGAAGCTG AAGGAGTTAC CCTACCACTT GCTCCATGCC
    GGCCGTATCG AGGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGACCTGGAT TTGCTGCCGG
    GGCCTCTCTG GGGGCATCGA GGGCCTGCTG GAAGACTTTG ACCTGTGCGC CCCATACATG
    GACTGCCCTG AGGTCAGCCT GGTCCGGGAA GCTCTCCAGC TGTGCCGCCC GGCAGTGGAG
    CTCCGAGGCA TGGAGAGAAG CATCCTGTAC ACAGAACTCC TGGCCAGACT CCATTTCTTC
    AGCACTTCAC ATCCCGGGCT GGTGGGACAG CTGTGCCAAC AGGCACAGAG CTGGTTCCGG
    ATGTGCCCAC AGCCTGTGCT GGTGCCCCTT GGAGGATTCC TCCAGCCCCC TGGAGGGCCT
    CTGCGGGCAA CCCTCAGCGG CTGTCACAAA GGCATCACTG CCATGGCATG GAGCCTGGAG
    GAGAAGCTGC TGCTGGTTGG CACGCAGGAG GGCATGGTGG CTGTGTGGGA CATGGAGGAA
    CAGCAGGTGA TCCACATCCT TCCTGGACAC ACAGGAGAGG TAAGGTGTGT GGAAGTGTTT
    GCCCAAGGGA CACATGCTGT CTCTGCCTCG AAAGACCAGA CCTTGCGCTT GTGGGACTTA
    CTGTCTGGCC AGGAGAAATT CACCATTTGG GATGGAGGTT CAAAAGATCC CACTGAATCT
    CAGACCTGGA GCCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTCAGC TTCAGGCTCA
    AAGATCAGTG CTTGGAACCT GGAAACTGCA GAGCTGATTT TCCGTATCCT GGGGGATGCC
    TCTGACCCCT GGGTATGCAT GGCAGTGCTG GCTTCCCAGG CCACACTGCT GACTGTGTCC
    CAGGGTGGCG TGATCAGTCT GTGGAGCTCA GCCACGGGAA CACTGCACAG GAGGCAACGT
    TTGTCCAGCG TCAAAGAAGA TACACTTGCC TGTGGGGTCT CAGTCCAGAA ACGAGGAATG
    ATGGTTACTG GGTCCAGCAC GGGCTCCATC TCTTTGGTTT CCTCCGAAGG AGACAGCCTG
    CTGGAAAAGC TTCCGGAAGC TGTGGGGTTC CTGGTGGTCT CTGAAGACGA GTCTCTTCTC
    GCTGCAGGTT TTGGAAGGTC CGTGAGGATA TTTTTGGCAG ATGCACAGGG ATTTCATCGA
    TTCCTGGCCA CTGACCTTGA ACATGAAGAT GTGGTAGAGA CAGCCGTTTT TGGTCCTGAA
    AACAATCTGA TTGTCACCGG GTCCCGGGAT GCACTCATTC AGGTGTGGAG TCTGTCAGCG
    CAGGGGACTC TGCTGGACAT CTTGGACGGT GTCGGGGCCC CTGTGAGCCT GCTGGCCCGG
    GGTGGGGCCT TGGTGGCATC TGCTTCCCGT CAGTCCTCAT CTTTTAAAGT CTGGGATCTC
    ACCTGTGCTC AGAAGCCGAG GGCCTCTAAC CCATTCTTGG ACCGCACTGG CCTCACAGCA
    GTGTCACACA ACGGCAGCTA CGTCTATTTC CCTAAAACTG GGGACAAAAA CAAAGTCAGC
    ATTTGGGACT TGGCAGAAGG TGAGGAGCAA GATGCTCTGG ACACCTCCAA CGAGGTCAGG
    TGTCTGGAGG TTGCCGAGCA GGCCAATCTC CTTTTCACTG GCCTCGTTTC GGGGATTGTC
    CTGGTGTTCC CCCTGAACTC CAGACAAGAC GTGATGTGTA TCCCTCCTCC AGAAGCCCGG
    AAAGCCGTCA ACTGCATGGC TCTTAGTAAG GACGAGGGGC ACCTGGCCAT TGCCTATGAC
    AGCACCGTCT TGGTGCTGGA CATCAGCCCG GGGGACCCCT GTCCAGTCAT CGACGGGCCA
    ATATACACCT TCTATACCCA ACTGCCTGAC ACCATAGCCA GTGTGGCCAT TCTGGCTGAC
    TGTCGTGTCA TCTACGGCAT GATCAACGGT GACCTCTTCC TTTACGAGTG TGTGAATTCC
    AAGGTGTTCC CTCTGGAGGC CCATGGGAGC CGAGTCACCT GTGTGGAGGT CAGCCACAAA
    GAGCAGCTGG CAGTCAGCGG GGCCGAGGAA GCCCTACTGT GTCTCTGGGA CCTGCAGGTG
    TGCAAGTGGA AATTCGAGAT GAGCTACACG GCACACTGCT GGCTGTCCAG TTTGTGCATG
    CCGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236142
    Clone ID Related Accession (Same CDS sequence) XM_015471888.2
    Accession Version XM_015471888.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4698bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT domain- and WD repeat-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471888.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGACGCAG AGAGGGAAGC CCTCCAGAGC ACCGCCTACC CCGAAGTGCA GACCTTCTGC 
    CAGAAGCACG GCCTGATGTT CGAGGTGGTT GACCTGAGGT GGGGTATTCG GAACACTGAG
    GCCACTAACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TTGACCGGTG TCGGAAAACA
    TCCATAGGGC CAGCTTTTGT TGCCCTCGTG GGTGACCAGT ACGGCCCCTG CCCCGTCCCC
    ACGCAGATCG AGGAAAAGGA GTGGGAGGCG CTGAAGGCTC AGCTGACCGC CAGGCCCCGC
    GACCTGGAGC TGGTGGCACG ATGCTTCTGG AGAGACGAGA ACGCGGTGCC CCCCGCCTAC
    CTGCTGCAGG CCCTGGGCAC TGGGGAGGCC TCTGGGCCTG AAGAGGCCAC CCTGCTCTCT
    GCACTGCGCT CCGGAGCCCA GGAGGCCCGC AGGCTGGGGC TCATCAGCCA GGAGCGGTGG
    TACTGCTACC ACCGGTCAGT CATTGAGTGG GAAATAGAAC GGGCCTTGCT GAGCTCAACG
    GTTGGGGATC ACGGGGCAAC AGTCTTCCTG AGAGAGATCC AAGACCTCAA TAAACACATC
    CTAGAAGACT GTGCCCTCAG AATGGTGGAC CGTCTTGCAG ATGGTTGCCT GGACACGGAT
    GCCCAGAACC TTCTCAGCAA CCTCAAGGGG CGCATCGCTG ACCGGCAGCC TGGAGTTCTC
    AAGGCCCACC ACCTAGCGTG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCC
    CGATACCTGA AGGAGCTGGG GGAGCAGTTT GTGGCCAGGG CCAACCATCA GGTTCTTGAG
    TGCCTCCGGG AGCTGGAGGC AGGCAGGCAG GAGTTGGCAT GGCTCTACCA GGAGATCCGC
    CACCACCTGG GACTCAGTGC CGAGGTCAAC CGGACCTTCT GTGGGCGCCA GGAGCTCCTG
    ACCCAGCTGG GCCAGCGACT GCGACAGAAT GATGGCCACC CCCACCCACC CCTGGTGCTT
    TTTGGACCCC CAGGGATTGG AAAGACGGCT CTGATGTGCA AGCTGGCTGA GCAAATGTCG
    GGGCTGCTCG GCCGCAAGAC AGTGACCATT CTGCGGCTTC TGGGGACGTC ACAGATGAGC
    TCTGAGGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG
    CCCCTGCCCC CTGCCCAGGT CCTAGAGGCC CACACCAGGC TGGTCCAGTT TTTCCACATC
    CTCCTCCACA CCATCTCCAG CCGAAACTTT GAGTCCCTCG TGATCCTGCT GGATTCGGTG
    GATGAGGTGG ACTCTGTCCA CTGTGCTAGG AGGATCCCCT GGCTGCCTCC CAAATGTCCT
    CCGAGGGTCC ACCTCATCGT CTCGGCCTGC TCTGGACAGC GGGGGGTTTT AGACACCATT
    CAGCAGGTGC TCACGGACCG AGAGGCTTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA
    GGGCAAGAGA TGATCCAGCT CCTGCTGTCT GCCTCGAGGA GGACACTGAG TCAGGTGCAG
    AGGGACATGC TCTGGGCCAG CCTCCCAGAG TGTGGACACC CAGGGCGGCT GAGGCTGGCT
    TTCGAGGAGG CCCGGAAGTG GGCCTCCTTC ACTGTGCCAG CCCCGCTGGC CTCCACTGCT
    AAGGAGGCGA TGCAACAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCG GCTCCTGGTG
    GCCCGCGTGC TGGGCTACAT TGTGTCTTCC CGGCAGGGGC TCTCAGAGGC GGAGTTAAAG
    GATGTTCTGT CCTTGGATGA TGAGGTCCTG CAGAGCGTGT ACCAAGACTG GACCCCACCC
    AGCAAAGAGC TTCTGCGCTT CCCACCCCTG CTGTGGGTGA GGCTTCGTCG AGATCTGGGT
    AACTGCTTGG CCAGGCGGCC CGTGGACGGC TTCACCCTTC TGGCCATTGC TCACAGACAG
    CTGACCGAGG TGGTCAGAGA GCTCTACCTG TCTGGGCCTG AGAAAGCCAA GAGTCACGGA
    GTCTTGGCCG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA TCAAGAAACT CATCACCCTG
    CCACTCGTGG GAAAACCCCT GAACCTGGAC CGCAAGGTGG CCCCTCAGCC CCTGTGGTTC
    TCCGACATGG TTGCAAACCT GAGGAAGCTG AAGGAGTTAC CCTACCACTT GCTCCATGCC
    GGCCGTATCG AGGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGACCTGGAT TTGCTGCCGG
    GGCCTCTCTG GGGGCATCGA GGGCCTGCTG GAAGACTTTG ACCTGTGCGC CCCATACATG
    GACTGCCCTG AGGTCAGCCT GGTCCGGGAA GCTCTCCAGC TGTGCCGCCC GGCAGTGGAG
    CTCCGAGGCA TGGAGAGAAG CATCCTGTAC ACAGAACTCC TGGCCAGACT CCATTTCTTC
    AGCACTTCAC ATCCCGGGCT GGTGGGACAG CTGTGCCAAC AGGCACAGAG CTGGTTCCGG
    ATGTGCCCAC AGCCTGTGCT GGTGCCCCTT GGAGGATTCC TCCAGCCCCC TGGAGGGCCT
    CTGCGGGCAA CCCTCAGCGG CTGTCACAAA GGCATCACTG CCATGGCATG GAGCCTGGAG
    GAGAAGCTGC TGCTGGTTGG CACGCAGGAG GGCATGGTGG CTGTGTGGGA CATGGAGGAA
    CAGCAGGTGA TCCACATCCT TCCTGGACAC ACAGGAGAGG TAAGGTGTGT GGAAGTGTTT
    GCCCAAGGGA CACATGCTGT CTCTGCCTCG AAAGACCAGA CCTTGCGCTT GTGGGACTTA
    CTGTCTGGCC AGGAGAAATT CACCATTTGG GATGGAGGTT CAAAAGATCC CACTGAATCT
    CAGACCTGGA GCCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTCAGC TTCAGGCTCA
    AAGATCAGTG CTTGGAACCT GGAAACTGCA GAGCTGATTT TCCGTATCCT GGGGGATGCC
    TCTGACCCCT GGGTATGCAT GGCAGTGCTG GCTTCCCAGG CCACACTGCT GACTGTGTCC
    CAGGGTGGCG TGATCAGTCT GTGGAGCTCA GCCACGGGAA CACTGCACAG GAGGCAACGT
    TTGTCCAGCG TCAAAGAAGA TACACTTGCC TGTGGGGTCT CAGTCCAGAA ACGAGGAATG
    ATGGTTACTG GGTCCAGCAC GGGCTCCATC TCTTTGGTTT CCTCCGAAGG AGACAGCCTG
    CTGGAAAAGC TTCCGGAAGC TGTGGGGTTC CTGGTGGTCT CTGAAGACGA GTCTCTTCTC
    GCTGCAGGTT TTGGAAGGTC CGTGAGGATA TTTTTGGCAG ATGCACAGGG ATTTCATCGA
    TTCCTGGCCA CTGACCTTGA ACATGAAGAT GTGGTAGAGA CAGCCGTTTT TGGTCCTGAA
    AACAATCTGA TTGTCACCGG GTCCCGGGAT GCACTCATTC AGGTGTGGAG TCTGTCAGCG
    CAGGGGACTC TGCTGGACAT CTTGGACGGT GTCGGGGCCC CTGTGAGCCT GCTGGCCCGG
    GGTGGGGCCT TGGTGGCATC TGCTTCCCGT CAGTCCTCAT CTTTTAAAGT CTGGGATCTC
    ACCTGTGCTC AGAAGCCGAG GGCCTCTAAC CCATTCTTGG ACCGCACTGG CCTCACAGCA
    GTGTCACACA ACGGCAGCTA CGTCTATTTC CCTAAAACTG GGGACAAAAA CAAAGTCAGC
    ATTTGGGACT TGGCAGAAGG TGAGGAGCAA GATGCTCTGG ACACCTCCAA CGAGGTCAGG
    TGTCTGGAGG TTGCCGAGCA GGCCAATCTC CTTTTCACTG GCCTCGTTTC GGGGATTGTC
    CTGGTGTTCC CCCTGAACTC CAGACAAGAC GTGATGTGTA TCCCTCCTCC AGAAGCCCGG
    AAAGCCGTCA ACTGCATGGC TCTTAGTAAG GACGAGGGGC ACCTGGCCAT TGCCTATGAC
    AGCACCGTCT TGGTGCTGGA CATCAGCCCG GGGGACCCCT GTCCAGTCAT CGACGGGCCA
    ATATACACCT TCTATACCCA ACTGCCTGAC ACCATAGCCA GTGTGGCCAT TCTGGCTGAC
    TGTCGTGTCA TCTACGGCAT GATCAACGGT GACCTCTTCC TTTACGAGTG TGTGAATTCC
    AAGGTGTTCC CTCTGGAGGC CCATGGGAGC CGAGTCACCT GTGTGGAGGT CAGCCACAAA
    GAGCAGCTGG CAGTCAGCGG GGCCGAGGAA GCCCTACTGT GTCTCTGGGA CCTGCAGGTG
    TGCAAGTGGA AATTCGAGAT GAGCTACACG AATTCTTACT GTATAGGAGT CCAATGTGCT
    TGCTTCTCCA AGGATGACAA GTATGTCTAC GCAGGCTTGA AAGATTGCTC CATAATTGTA
    TGGAGTGTGC TGGATGGCAC ACTGCTGGCT GTCCAGTTTG TGCATGCCGT GGTGAACAGA
    ATCATCCCAA CCTCCAGTGG CTTCATTGCC CCCTCCAGAC ACGGCTATCT CATCCGTGAG
    CGTTTCCAGT GCCCTTCATC AGGAATCTCA CAACAGGACC CTCTCAAGAA CTTCAAGAAA
    GCAGTGTGGA TGGTCAAATC CAGGCAGAGA GAAGAGCTGG CAGCTGCTGC AGGAGCACTC
    CAGGACTTGA GATCGGAGAG TGCCCAGGGA AATGAATCCA GATCAAACAA ACGCTCACAA
    GTCTGCCTGC TGGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327374.2
    CDS548..5245
    Translation

    Target ORF information:

    RefSeq Version XM_015471888.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471888.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGACGCAG AGAGGGAAGC CCTCCAGAGC ACCGCCTACC CCGAAGTGCA GACCTTCTGC 
    CAGAAGCACG GCCTGATGTT CGAGGTGGTT GACCTGAGGT GGGGTATTCG GAACACTGAG
    GCCACTAACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TTGACCGGTG TCGGAAAACA
    TCCATAGGGC CAGCTTTTGT TGCCCTCGTG GGTGACCAGT ACGGCCCCTG CCCCGTCCCC
    ACGCAGATCG AGGAAAAGGA GTGGGAGGCG CTGAAGGCTC AGCTGACCGC CAGGCCCCGC
    GACCTGGAGC TGGTGGCACG ATGCTTCTGG AGAGACGAGA ACGCGGTGCC CCCCGCCTAC
    CTGCTGCAGG CCCTGGGCAC TGGGGAGGCC TCTGGGCCTG AAGAGGCCAC CCTGCTCTCT
    GCACTGCGCT CCGGAGCCCA GGAGGCCCGC AGGCTGGGGC TCATCAGCCA GGAGCGGTGG
    TACTGCTACC ACCGGTCAGT CATTGAGTGG GAAATAGAAC GGGCCTTGCT GAGCTCAACG
    GTTGGGGATC ACGGGGCAAC AGTCTTCCTG AGAGAGATCC AAGACCTCAA TAAACACATC
    CTAGAAGACT GTGCCCTCAG AATGGTGGAC CGTCTTGCAG ATGGTTGCCT GGACACGGAT
    GCCCAGAACC TTCTCAGCAA CCTCAAGGGG CGCATCGCTG ACCGGCAGCC TGGAGTTCTC
    AAGGCCCACC ACCTAGCGTG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCC
    CGATACCTGA AGGAGCTGGG GGAGCAGTTT GTGGCCAGGG CCAACCATCA GGTTCTTGAG
    TGCCTCCGGG AGCTGGAGGC AGGCAGGCAG GAGTTGGCAT GGCTCTACCA GGAGATCCGC
    CACCACCTGG GACTCAGTGC CGAGGTCAAC CGGACCTTCT GTGGGCGCCA GGAGCTCCTG
    ACCCAGCTGG GCCAGCGACT GCGACAGAAT GATGGCCACC CCCACCCACC CCTGGTGCTT
    TTTGGACCCC CAGGGATTGG AAAGACGGCT CTGATGTGCA AGCTGGCTGA GCAAATGTCG
    GGGCTGCTCG GCCGCAAGAC AGTGACCATT CTGCGGCTTC TGGGGACGTC ACAGATGAGC
    TCTGAGGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG
    CCCCTGCCCC CTGCCCAGGT CCTAGAGGCC CACACCAGGC TGGTCCAGTT TTTCCACATC
    CTCCTCCACA CCATCTCCAG CCGAAACTTT GAGTCCCTCG TGATCCTGCT GGATTCGGTG
    GATGAGGTGG ACTCTGTCCA CTGTGCTAGG AGGATCCCCT GGCTGCCTCC CAAATGTCCT
    CCGAGGGTCC ACCTCATCGT CTCGGCCTGC TCTGGACAGC GGGGGGTTTT AGACACCATT
    CAGCAGGTGC TCACGGACCG AGAGGCTTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA
    GGGCAAGAGA TGATCCAGCT CCTGCTGTCT GCCTCGAGGA GGACACTGAG TCAGGTGCAG
    AGGGACATGC TCTGGGCCAG CCTCCCAGAG TGTGGACACC CAGGGCGGCT GAGGCTGGCT
    TTCGAGGAGG CCCGGAAGTG GGCCTCCTTC ACTGTGCCAG CCCCGCTGGC CTCCACTGCT
    AAGGAGGCGA TGCAACAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCG GCTCCTGGTG
    GCCCGCGTGC TGGGCTACAT TGTGTCTTCC CGGCAGGGGC TCTCAGAGGC GGAGTTAAAG
    GATGTTCTGT CCTTGGATGA TGAGGTCCTG CAGAGCGTGT ACCAAGACTG GACCCCACCC
    AGCAAAGAGC TTCTGCGCTT CCCACCCCTG CTGTGGGTGA GGCTTCGTCG AGATCTGGGT
    AACTGCTTGG CCAGGCGGCC CGTGGACGGC TTCACCCTTC TGGCCATTGC TCACAGACAG
    CTGACCGAGG TGGTCAGAGA GCTCTACCTG TCTGGGCCTG AGAAAGCCAA GAGTCACGGA
    GTCTTGGCCG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA TCAAGAAACT CATCACCCTG
    CCACTCGTGG GAAAACCCCT GAACCTGGAC CGCAAGGTGG CCCCTCAGCC CCTGTGGTTC
    TCCGACATGG TTGCAAACCT GAGGAAGCTG AAGGAGTTAC CCTACCACTT GCTCCATGCC
    GGCCGTATCG AGGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGACCTGGAT TTGCTGCCGG
    GGCCTCTCTG GGGGCATCGA GGGCCTGCTG GAAGACTTTG ACCTGTGCGC CCCATACATG
    GACTGCCCTG AGGTCAGCCT GGTCCGGGAA GCTCTCCAGC TGTGCCGCCC GGCAGTGGAG
    CTCCGAGGCA TGGAGAGAAG CATCCTGTAC ACAGAACTCC TGGCCAGACT CCATTTCTTC
    AGCACTTCAC ATCCCGGGCT GGTGGGACAG CTGTGCCAAC AGGCACAGAG CTGGTTCCGG
    ATGTGCCCAC AGCCTGTGCT GGTGCCCCTT GGAGGATTCC TCCAGCCCCC TGGAGGGCCT
    CTGCGGGCAA CCCTCAGCGG CTGTCACAAA GGCATCACTG CCATGGCATG GAGCCTGGAG
    GAGAAGCTGC TGCTGGTTGG CACGCAGGAG GGCATGGTGG CTGTGTGGGA CATGGAGGAA
    CAGCAGGTGA TCCACATCCT TCCTGGACAC ACAGGAGAGG TAAGGTGTGT GGAAGTGTTT
    GCCCAAGGGA CACATGCTGT CTCTGCCTCG AAAGACCAGA CCTTGCGCTT GTGGGACTTA
    CTGTCTGGCC AGGAGAAATT CACCATTTGG GATGGAGGTT CAAAAGATCC CACTGAATCT
    CAGACCTGGA GCCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTCAGC TTCAGGCTCA
    AAGATCAGTG CTTGGAACCT GGAAACTGCA GAGCTGATTT TCCGTATCCT GGGGGATGCC
    TCTGACCCCT GGGTATGCAT GGCAGTGCTG GCTTCCCAGG CCACACTGCT GACTGTGTCC
    CAGGGTGGCG TGATCAGTCT GTGGAGCTCA GCCACGGGAA CACTGCACAG GAGGCAACGT
    TTGTCCAGCG TCAAAGAAGA TACACTTGCC TGTGGGGTCT CAGTCCAGAA ACGAGGAATG
    ATGGTTACTG GGTCCAGCAC GGGCTCCATC TCTTTGGTTT CCTCCGAAGG AGACAGCCTG
    CTGGAAAAGC TTCCGGAAGC TGTGGGGTTC CTGGTGGTCT CTGAAGACGA GTCTCTTCTC
    GCTGCAGGTT TTGGAAGGTC CGTGAGGATA TTTTTGGCAG ATGCACAGGG ATTTCATCGA
    TTCCTGGCCA CTGACCTTGA ACATGAAGAT GTGGTAGAGA CAGCCGTTTT TGGTCCTGAA
    AACAATCTGA TTGTCACCGG GTCCCGGGAT GCACTCATTC AGGTGTGGAG TCTGTCAGCG
    CAGGGGACTC TGCTGGACAT CTTGGACGGT GTCGGGGCCC CTGTGAGCCT GCTGGCCCGG
    GGTGGGGCCT TGGTGGCATC TGCTTCCCGT CAGTCCTCAT CTTTTAAAGT CTGGGATCTC
    ACCTGTGCTC AGAAGCCGAG GGCCTCTAAC CCATTCTTGG ACCGCACTGG CCTCACAGCA
    GTGTCACACA ACGGCAGCTA CGTCTATTTC CCTAAAACTG GGGACAAAAA CAAAGTCAGC
    ATTTGGGACT TGGCAGAAGG TGAGGAGCAA GATGCTCTGG ACACCTCCAA CGAGGTCAGG
    TGTCTGGAGG TTGCCGAGCA GGCCAATCTC CTTTTCACTG GCCTCGTTTC GGGGATTGTC
    CTGGTGTTCC CCCTGAACTC CAGACAAGAC GTGATGTGTA TCCCTCCTCC AGAAGCCCGG
    AAAGCCGTCA ACTGCATGGC TCTTAGTAAG GACGAGGGGC ACCTGGCCAT TGCCTATGAC
    AGCACCGTCT TGGTGCTGGA CATCAGCCCG GGGGACCCCT GTCCAGTCAT CGACGGGCCA
    ATATACACCT TCTATACCCA ACTGCCTGAC ACCATAGCCA GTGTGGCCAT TCTGGCTGAC
    TGTCGTGTCA TCTACGGCAT GATCAACGGT GACCTCTTCC TTTACGAGTG TGTGAATTCC
    AAGGTGTTCC CTCTGGAGGC CCATGGGAGC CGAGTCACCT GTGTGGAGGT CAGCCACAAA
    GAGCAGCTGG CAGTCAGCGG GGCCGAGGAA GCCCTACTGT GTCTCTGGGA CCTGCAGGTG
    TGCAAGTGGA AATTCGAGAT GAGCTACACG AATTCTTACT GTATAGGAGT CCAATGTGCT
    TGCTTCTCCA AGGATGACAA GTATGTCTAC GCAGGCTTGA AAGATTGCTC CATAATTGTA
    TGGAGTGTGC TGGATGGCAC ACTGCTGGCT GTCCAGTTTG TGCATGCCGT GGTGAACAGA
    ATCATCCCAA CCTCCAGTGG CTTCATTGCC CCCTCCAGAC ACGGCTATCT CATCCGTGAG
    CGTTTCCAGT GCCCTTCATC AGGAATCTCA CAACAGGACC CTCTCAAGAA CTTCAAGAAA
    GCAGTGTGGA TGGTCAAATC CAGGCAGAGA GAAGAGCTGG CAGCTGCTGC AGGAGCACTC
    CAGGACTTGA GATCGGAGAG TGCCCAGGGA AATGAATCCA GATCAAACAA ACGCTCACAA
    GTCTGCCTGC TGGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236143
    Clone ID Related Accession (Same CDS sequence) XM_024994419.1
    Accession Version XM_024994419.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4581bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT domain- and WD repeat-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGACGCAG AGAGGGAAGC CCTCCAGAGC ACCGCCTACC CCGAAGTGCA GACCTTCTGC 
    CAGAAGCACG GCCTGATGTT CGAGGCCCTC GTGGGTGACC AGTACGGCCC CTGCCCCGTC
    CCCACGCAGA TCGAGGAAAA GGAGTGGGAG GCGCTGAAGG CTCAGCTGAC CGCCAGGCCC
    CGCGACCTGG AGCTGGTGGC ACGATGCTTC TGGAGAGACG AGAACGCGGT GCCCCCCGCC
    TACCTGCTGC AGGCCCTGGG CACTGGGGAG GCCTCTGGGC CTGAAGAGGC CACCCTGCTC
    TCTGCACTGC GCTCCGGAGC CCAGGAGGCC CGCAGGCTGG GGCTCATCAG CCAGGAGCGG
    TGGTACTGCT ACCACCGGTC AGTCATTGAG TGGGAAATAG AACGGGCCTT GCTGAGCTCA
    ACGGTTGGGG ATCACGGGGC AACAGTCTTC CTGAGAGAGA TCCAAGACCT CAATAAACAC
    ATCCTAGAAG ACTGTGCCCT CAGAATGGTG GACCGTCTTG CAGATGGTTG CCTGGACACG
    GATGCCCAGA ACCTTCTCAG CAACCTCAAG GGGCGCATCG CTGACCGGCA GCCTGGAGTT
    CTCAAGGCCC ACCACCTAGC GTGGAGCCGA GACCTGGTGA ACCCCAAAAA CAAGGCTCAT
    GCCCGATACC TGAAGGAGCT GGGGGAGCAG TTTGTGGCCA GGGCCAACCA TCAGGTTCTT
    GAGTGCCTCC GGGAGCTGGA GGCAGGCAGG CAGGAGTTGG CATGGCTCTA CCAGGAGATC
    CGCCACCACC TGGGACTCAG TGCCGAGGTC AACCGGACCT TCTGTGGGCG CCAGGAGCTC
    CTGACCCAGC TGGGCCAGCG ACTGCGACAG AATGATGGCC ACCCCCACCC ACCCCTGGTG
    CTTTTTGGAC CCCCAGGGAT TGGAAAGACG GCTCTGATGT GCAAGCTGGC TGAGCAAATG
    TCGGGGCTGC TCGGCCGCAA GACAGTGACC ATTCTGCGGC TTCTGGGGAC GTCACAGATG
    AGCTCTGAGG CCCGAGGCCT GCTCCAGAGC ATCTGCTTCC AGGTGTGCCT GGCCTACGGG
    CTGCCCCTGC CCCCTGCCCA GGTCCTAGAG GCCCACACCA GGCTGGTCCA GTTTTTCCAC
    ATCCTCCTCC ACACCATCTC CAGCCGAAAC TTTGAGTCCC TCGTGATCCT GCTGGATTCG
    GTGGATGAGG TGGACTCTGT CCACTGTGCT AGGAGGATCC CCTGGCTGCC TCCCAAATGT
    CCTCCGAGGG TCCACCTCAT CGTCTCGGCC TGCTCTGGAC AGCGGGGGGT TTTAGACACC
    ATTCAGCAGG TGCTCACGGA CCGAGAGGCT TACTGGGAGG TGAAACCCCT CTCCGGAAAC
    CAAGGGCAAG AGATGATCCA GCTCCTGCTG TCTGCCTCGA GGAGGACACT GAGTCAGGTG
    CAGAGGGACA TGCTCTGGGC CAGCCTCCCA GAGTGTGGAC ACCCAGGGCG GCTGAGGCTG
    GCTTTCGAGG AGGCCCGGAA GTGGGCCTCC TTCACTGTGC CAGCCCCGCT GGCCTCCACT
    GCTAAGGAGG CGATGCAACA GCTGTGTGCC CGCCTGGAGC AGACACACGG GCGGCTCCTG
    GTGGCCCGCG TGCTGGGCTA CATTGTGTCT TCCCGGCAGG GGCTCTCAGA GGCGGAGTTA
    AAGGATGTTC TGTCCTTGGA TGATGAGGTC CTGCAGAGCG TGTACCAAGA CTGGACCCCA
    CCCAGCAAAG AGCTTCTGCG CTTCCCACCC CTGCTGTGGG TGAGGCTTCG TCGAGATCTG
    GGTAACTGCT TGGCCAGGCG GCCCGTGGAC GGCTTCACCC TTCTGGCCAT TGCTCACAGA
    CAGCTGACCG AGGTGGTCAG AGAGCTCTAC CTGTCTGGGC CTGAGAAAGC CAAGAGTCAC
    GGAGTCTTGG CCGACTTCTT CTCAGGGGCC TGGAGCCAGG GCATCAAGAA ACTCATCACC
    CTGCCACTCG TGGGAAAACC CCTGAACCTG GACCGCAAGG TGGCCCCTCA GCCCCTGTGG
    TTCTCCGACA TGGTTGCAAA CCTGAGGAAG CTGAAGGAGT TACCCTACCA CTTGCTCCAT
    GCCGGCCGTA TCGAGGAGCT GAAGCAGGAG GTTCTGGGCA GCATGACCTG GATTTGCTGC
    CGGGGCCTCT CTGGGGGCAT CGAGGGCCTG CTGGAAGACT TTGACCTGTG CGCCCCATAC
    ATGGACTGCC CTGAGGTCAG CCTGGTCCGG GAAGCTCTCC AGCTGTGCCG CCCGGCAGTG
    GAGCTCCGAG GCATGGAGAG AAGCATCCTG TACACAGAAC TCCTGGCCAG ACTCCATTTC
    TTCAGCACTT CACATCCCGG GCTGGTGGGA CAGCTGTGCC AACAGGCACA GAGCTGGTTC
    CGGATGTGCC CACAGCCTGT GCTGGTGCCC CTTGGAGGAT TCCTCCAGCC CCCTGGAGGG
    CCTCTGCGGG CAACCCTCAG CGGCTGTCAC AAAGGCATCA CTGCCATGGC ATGGAGCCTG
    GAGGAGAAGC TGCTGCTGGT TGGCACGCAG GAGGGCATGG TGGCTGTGTG GGACATGGAG
    GAACAGCAGG TGATCCACAT CCTTCCTGGA CACACAGGAG AGGTAAGGTG TGTGGAAGTG
    TTTGCCCAAG GGACACATGC TGTCTCTGCC TCGAAAGACC AGACCTTGCG CTTGTGGGAC
    TTACTGTCTG GCCAGGAGAA ATTCACCATT TGGGATGGAG GTTCAAAAGA TCCCACTGAA
    TCTCAGACCT GGAGCCTTCA TGTGGATGAA GCAAAGAAGG TTGTGTATTC AGCTTCAGGC
    TCAAAGATCA GTGCTTGGAA CCTGGAAACT GCAGAGCTGA TTTTCCGTAT CCTGGGGGAT
    GCCTCTGACC CCTGGGTATG CATGGCAGTG CTGGCTTCCC AGGCCACACT GCTGACTGTG
    TCCCAGGGTG GCGTGATCAG TCTGTGGAGC TCAGCCACGG GAACACTGCA CAGGAGGCAA
    CGTTTGTCCA GCGTCAAAGA AGATACACTT GCCTGTGGGG TCTCAGTCCA GAAACGAGGA
    ATGATGGTTA CTGGGTCCAG CACGGGCTCC ATCTCTTTGG TTTCCTCCGA AGGAGACAGC
    CTGCTGGAAA AGCTTCCGGA AGCTGTGGGG TTCCTGGTGG TCTCTGAAGA CGAGTCTCTT
    CTCGCTGCAG GTTTTGGAAG GTCCGTGAGG ATATTTTTGG CAGATGCACA GGGATTTCAT
    CGATTCCTGG CCACTGACCT TGAACATGAA GATGTGGTAG AGACAGCCGT TTTTGGTCCT
    GAAAACAATC TGATTGTCAC CGGGTCCCGG GATGCACTCA TTCAGGTGTG GAGTCTGTCA
    GCGCAGGGGA CTCTGCTGGA CATCTTGGAC GGTGTCGGGG CCCCTGTGAG CCTGCTGGCC
    CGGGGTGGGG CCTTGGTGGC ATCTGCTTCC CGTCAGTCCT CATCTTTTAA AGTCTGGGAT
    CTCACCTGTG CTCAGAAGCC GAGGGCCTCT AACCCATTCT TGGACCGCAC TGGCCTCACA
    GCAGTGTCAC ACAACGGCAG CTACGTCTAT TTCCCTAAAA CTGGGGACAA AAACAAAGTC
    AGCATTTGGG ACTTGGCAGA AGGTGAGGAG CAAGATGCTC TGGACACCTC CAACGAGGTC
    AGGTGTCTGG AGGTTGCCGA GCAGGCCAAT CTCCTTTTCA CTGGCCTCGT TTCGGGGATT
    GTCCTGGTGT TCCCCCTGAA CTCCAGACAA GACGTGATGT GTATCCCTCC TCCAGAAGCC
    CGGAAAGCCG TCAACTGCAT GGCTCTTAGT AAGGACGAGG GGCACCTGGC CATTGCCTAT
    GACAGCACCG TCTTGGTGCT GGACATCAGC CCGGGGGACC CCTGTCCAGT CATCGACGGG
    CCAATATACA CCTTCTATAC CCAACTGCCT GACACCATAG CCAGTGTGGC CATTCTGGCT
    GACTGTCGTG TCATCTACGG CATGATCAAC GGTGACCTCT TCCTTTACGA GTGTGTGAAT
    TCCAAGGTGT TCCCTCTGGA GGCCCATGGG AGCCGAGTCA CCTGTGTGGA GGTCAGCCAC
    AAAGAGCAGC TGGCAGTCAG CGGGGCCGAG GAAGCCCTAC TGTGTCTCTG GGACCTGCAG
    GTGTGCAAGT GGAAATTCGA GATGAGCTAC ACGAATTCTT ACTGTATAGG AGTCCAATGT
    GCTTGCTTCT CCAAGGATGA CAAGTATGTC TACGCAGGCT TGAAAGATTG CTCCATAATT
    GTATGGAGTG TGCTGGATGG CACACTGCTG GCTGTCCAGT TTGTGCATGC CGTGGTGAAC
    AGAATCATCC CAACCTCCAG TGGCTTCATT GCCCCCTCCA GACACGGCTA TCTCATCCGT
    GAGCGTTTCC AGTGCCCTTC ATCAGGAATC TCACAACAGG ACCCTCTCAA GAACTTCAAG
    AAAGCAGTGT GGATGGTCAA ATCCAGGCAG AGAGAAGAGC TGGCAGCTGC TGCAGGAGCA
    CTCCAGGACT TGAGATCGGA GAGTGCCCAG GGAAATGAAT CCAGATCAAA CAAACGCTCA
    CAAGTCTGCC TGCTGGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850187.1
    CDS548..5128
    Translation

    Target ORF information:

    RefSeq Version XM_024994419.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994419.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGACGCAG AGAGGGAAGC CCTCCAGAGC ACCGCCTACC CCGAAGTGCA GACCTTCTGC 
    CAGAAGCACG GCCTGATGTT CGAGGCCCTC GTGGGTGACC AGTACGGCCC CTGCCCCGTC
    CCCACGCAGA TCGAGGAAAA GGAGTGGGAG GCGCTGAAGG CTCAGCTGAC CGCCAGGCCC
    CGCGACCTGG AGCTGGTGGC ACGATGCTTC TGGAGAGACG AGAACGCGGT GCCCCCCGCC
    TACCTGCTGC AGGCCCTGGG CACTGGGGAG GCCTCTGGGC CTGAAGAGGC CACCCTGCTC
    TCTGCACTGC GCTCCGGAGC CCAGGAGGCC CGCAGGCTGG GGCTCATCAG CCAGGAGCGG
    TGGTACTGCT ACCACCGGTC AGTCATTGAG TGGGAAATAG AACGGGCCTT GCTGAGCTCA
    ACGGTTGGGG ATCACGGGGC AACAGTCTTC CTGAGAGAGA TCCAAGACCT CAATAAACAC
    ATCCTAGAAG ACTGTGCCCT CAGAATGGTG GACCGTCTTG CAGATGGTTG CCTGGACACG
    GATGCCCAGA ACCTTCTCAG CAACCTCAAG GGGCGCATCG CTGACCGGCA GCCTGGAGTT
    CTCAAGGCCC ACCACCTAGC GTGGAGCCGA GACCTGGTGA ACCCCAAAAA CAAGGCTCAT
    GCCCGATACC TGAAGGAGCT GGGGGAGCAG TTTGTGGCCA GGGCCAACCA TCAGGTTCTT
    GAGTGCCTCC GGGAGCTGGA GGCAGGCAGG CAGGAGTTGG CATGGCTCTA CCAGGAGATC
    CGCCACCACC TGGGACTCAG TGCCGAGGTC AACCGGACCT TCTGTGGGCG CCAGGAGCTC
    CTGACCCAGC TGGGCCAGCG ACTGCGACAG AATGATGGCC ACCCCCACCC ACCCCTGGTG
    CTTTTTGGAC CCCCAGGGAT TGGAAAGACG GCTCTGATGT GCAAGCTGGC TGAGCAAATG
    TCGGGGCTGC TCGGCCGCAA GACAGTGACC ATTCTGCGGC TTCTGGGGAC GTCACAGATG
    AGCTCTGAGG CCCGAGGCCT GCTCCAGAGC ATCTGCTTCC AGGTGTGCCT GGCCTACGGG
    CTGCCCCTGC CCCCTGCCCA GGTCCTAGAG GCCCACACCA GGCTGGTCCA GTTTTTCCAC
    ATCCTCCTCC ACACCATCTC CAGCCGAAAC TTTGAGTCCC TCGTGATCCT GCTGGATTCG
    GTGGATGAGG TGGACTCTGT CCACTGTGCT AGGAGGATCC CCTGGCTGCC TCCCAAATGT
    CCTCCGAGGG TCCACCTCAT CGTCTCGGCC TGCTCTGGAC AGCGGGGGGT TTTAGACACC
    ATTCAGCAGG TGCTCACGGA CCGAGAGGCT TACTGGGAGG TGAAACCCCT CTCCGGAAAC
    CAAGGGCAAG AGATGATCCA GCTCCTGCTG TCTGCCTCGA GGAGGACACT GAGTCAGGTG
    CAGAGGGACA TGCTCTGGGC CAGCCTCCCA GAGTGTGGAC ACCCAGGGCG GCTGAGGCTG
    GCTTTCGAGG AGGCCCGGAA GTGGGCCTCC TTCACTGTGC CAGCCCCGCT GGCCTCCACT
    GCTAAGGAGG CGATGCAACA GCTGTGTGCC CGCCTGGAGC AGACACACGG GCGGCTCCTG
    GTGGCCCGCG TGCTGGGCTA CATTGTGTCT TCCCGGCAGG GGCTCTCAGA GGCGGAGTTA
    AAGGATGTTC TGTCCTTGGA TGATGAGGTC CTGCAGAGCG TGTACCAAGA CTGGACCCCA
    CCCAGCAAAG AGCTTCTGCG CTTCCCACCC CTGCTGTGGG TGAGGCTTCG TCGAGATCTG
    GGTAACTGCT TGGCCAGGCG GCCCGTGGAC GGCTTCACCC TTCTGGCCAT TGCTCACAGA
    CAGCTGACCG AGGTGGTCAG AGAGCTCTAC CTGTCTGGGC CTGAGAAAGC CAAGAGTCAC
    GGAGTCTTGG CCGACTTCTT CTCAGGGGCC TGGAGCCAGG GCATCAAGAA ACTCATCACC
    CTGCCACTCG TGGGAAAACC CCTGAACCTG GACCGCAAGG TGGCCCCTCA GCCCCTGTGG
    TTCTCCGACA TGGTTGCAAA CCTGAGGAAG CTGAAGGAGT TACCCTACCA CTTGCTCCAT
    GCCGGCCGTA TCGAGGAGCT GAAGCAGGAG GTTCTGGGCA GCATGACCTG GATTTGCTGC
    CGGGGCCTCT CTGGGGGCAT CGAGGGCCTG CTGGAAGACT TTGACCTGTG CGCCCCATAC
    ATGGACTGCC CTGAGGTCAG CCTGGTCCGG GAAGCTCTCC AGCTGTGCCG CCCGGCAGTG
    GAGCTCCGAG GCATGGAGAG AAGCATCCTG TACACAGAAC TCCTGGCCAG ACTCCATTTC
    TTCAGCACTT CACATCCCGG GCTGGTGGGA CAGCTGTGCC AACAGGCACA GAGCTGGTTC
    CGGATGTGCC CACAGCCTGT GCTGGTGCCC CTTGGAGGAT TCCTCCAGCC CCCTGGAGGG
    CCTCTGCGGG CAACCCTCAG CGGCTGTCAC AAAGGCATCA CTGCCATGGC ATGGAGCCTG
    GAGGAGAAGC TGCTGCTGGT TGGCACGCAG GAGGGCATGG TGGCTGTGTG GGACATGGAG
    GAACAGCAGG TGATCCACAT CCTTCCTGGA CACACAGGAG AGGTAAGGTG TGTGGAAGTG
    TTTGCCCAAG GGACACATGC TGTCTCTGCC TCGAAAGACC AGACCTTGCG CTTGTGGGAC
    TTACTGTCTG GCCAGGAGAA ATTCACCATT TGGGATGGAG GTTCAAAAGA TCCCACTGAA
    TCTCAGACCT GGAGCCTTCA TGTGGATGAA GCAAAGAAGG TTGTGTATTC AGCTTCAGGC
    TCAAAGATCA GTGCTTGGAA CCTGGAAACT GCAGAGCTGA TTTTCCGTAT CCTGGGGGAT
    GCCTCTGACC CCTGGGTATG CATGGCAGTG CTGGCTTCCC AGGCCACACT GCTGACTGTG
    TCCCAGGGTG GCGTGATCAG TCTGTGGAGC TCAGCCACGG GAACACTGCA CAGGAGGCAA
    CGTTTGTCCA GCGTCAAAGA AGATACACTT GCCTGTGGGG TCTCAGTCCA GAAACGAGGA
    ATGATGGTTA CTGGGTCCAG CACGGGCTCC ATCTCTTTGG TTTCCTCCGA AGGAGACAGC
    CTGCTGGAAA AGCTTCCGGA AGCTGTGGGG TTCCTGGTGG TCTCTGAAGA CGAGTCTCTT
    CTCGCTGCAG GTTTTGGAAG GTCCGTGAGG ATATTTTTGG CAGATGCACA GGGATTTCAT
    CGATTCCTGG CCACTGACCT TGAACATGAA GATGTGGTAG AGACAGCCGT TTTTGGTCCT
    GAAAACAATC TGATTGTCAC CGGGTCCCGG GATGCACTCA TTCAGGTGTG GAGTCTGTCA
    GCGCAGGGGA CTCTGCTGGA CATCTTGGAC GGTGTCGGGG CCCCTGTGAG CCTGCTGGCC
    CGGGGTGGGG CCTTGGTGGC ATCTGCTTCC CGTCAGTCCT CATCTTTTAA AGTCTGGGAT
    CTCACCTGTG CTCAGAAGCC GAGGGCCTCT AACCCATTCT TGGACCGCAC TGGCCTCACA
    GCAGTGTCAC ACAACGGCAG CTACGTCTAT TTCCCTAAAA CTGGGGACAA AAACAAAGTC
    AGCATTTGGG ACTTGGCAGA AGGTGAGGAG CAAGATGCTC TGGACACCTC CAACGAGGTC
    AGGTGTCTGG AGGTTGCCGA GCAGGCCAAT CTCCTTTTCA CTGGCCTCGT TTCGGGGATT
    GTCCTGGTGT TCCCCCTGAA CTCCAGACAA GACGTGATGT GTATCCCTCC TCCAGAAGCC
    CGGAAAGCCG TCAACTGCAT GGCTCTTAGT AAGGACGAGG GGCACCTGGC CATTGCCTAT
    GACAGCACCG TCTTGGTGCT GGACATCAGC CCGGGGGACC CCTGTCCAGT CATCGACGGG
    CCAATATACA CCTTCTATAC CCAACTGCCT GACACCATAG CCAGTGTGGC CATTCTGGCT
    GACTGTCGTG TCATCTACGG CATGATCAAC GGTGACCTCT TCCTTTACGA GTGTGTGAAT
    TCCAAGGTGT TCCCTCTGGA GGCCCATGGG AGCCGAGTCA CCTGTGTGGA GGTCAGCCAC
    AAAGAGCAGC TGGCAGTCAG CGGGGCCGAG GAAGCCCTAC TGTGTCTCTG GGACCTGCAG
    GTGTGCAAGT GGAAATTCGA GATGAGCTAC ACGAATTCTT ACTGTATAGG AGTCCAATGT
    GCTTGCTTCT CCAAGGATGA CAAGTATGTC TACGCAGGCT TGAAAGATTG CTCCATAATT
    GTATGGAGTG TGCTGGATGG CACACTGCTG GCTGTCCAGT TTGTGCATGC CGTGGTGAAC
    AGAATCATCC CAACCTCCAG TGGCTTCATT GCCCCCTCCA GACACGGCTA TCTCATCCGT
    GAGCGTTTCC AGTGCCCTTC ATCAGGAATC TCACAACAGG ACCCTCTCAA GAACTTCAAG
    AAAGCAGTGT GGATGGTCAA ATCCAGGCAG AGAGAAGAGC TGGCAGCTGC TGCAGGAGCA
    CTCCAGGACT TGAGATCGGA GAGTGCCCAG GGAAATGAAT CCAGATCAAA CAAACGCTCA
    CAAGTCTGCC TGCTGGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91036
    Clone ID Related Accession (Same CDS sequence) XM_015471888.1
    Accession Version XM_015471888.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT domain- and WD repeat-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104002.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTCGAGG TGGTTGACCT GAGGTGGGGT ATTCGGAACA CTGAGGCCAC TAACCACATG 
    ACCACAGAAC TCTGCTTGGA GGAGCTTGAC CGGTGTCGGA AAACATCCAT AGGGCCAGCT
    TTTGTTGCCC TCGTGGGTGA CCAGTACGGC CCCTGCCCCG TCCCCACGCA GATCGAGGAA
    AAGGAGTGGG AGGCGCTGAA GGCTCAGCTG ACCGCCAGGC CCCGCGACCT GGAGCTGGTG
    GCACGATGCT TCTGGAGAGA CGAGAACGCG GTGCCCCCCG CCTACCTGCT GCAGGCCCTG
    GGCACTGGGG AGGCCTCTGG GCCTGAAGAG GCCACCCTGC TCTCTGCACT GCGCTCCGGA
    GCCCAGGAGG CCCGCAGGCT GGGGCTCATC AGCCAGGAGC GGTGGTACTG CTACCACCGG
    TCAGTCATTG AGTGGGAAAT AGAACAGGCC TTGCTGAGCT CAACGGTTGG GGATCACGGG
    GCAACAGTCT TCCTGAGAGA GACCCAAGAC CTCAATAAAC ACATCCTAGA AGACTGTGCC
    CTCAGAATGG TGGACCGTCT TGCAGATGGT TGCCTGGACA CGGATGCCCA GCACCTTCTC
    AGCAACCTCA AGGGGCGCAT CGCTGACCGG CAGCCTGGAG TTCTCAAGGC CCACCACCTA
    GCGTGGAGCC GAGACCTGGT GAACCCCAAA AACAAGGCTC ATGCCCGATA CCTGAAGGAG
    CTGGGGGAGC AGTTTGTGGC CAGGGCCAAC CATCAGGTTC TTGAGTGCCT CCGGGAGCTG
    GAGGCAGGCA GGCAGGAGTT GGCATGGCTC TACCAGGAGA TCCGCCACCA CCTGGGACTC
    AGTGCCGAGG TCAACCGGAC CTTCTGTGGG CGCCAGGAGC TCCTGACCCA GCTGGGCCAG
    CGACTGCGAC AGAATGATGG CCACCCCCAC CCACCCCTGG TGCTTTTTGG ACCCCCAGGG
    ATTGGAAAGA CGGCTCTGAT GTGCAAGCTG GCTGAGCAAA TGTCGGGGCT GCTCGGCCGC
    AAGACAGTGA CCATTCTGCG GCTTCTGGGG ACGTCACAGA TGAGCTCTGA GGCCCGAGGC
    CTGCTCCAGA GCATCTGCTT CCAGGTGTGC CTGGCCTACG GGCTGCCCCT GCCCCCTGCC
    CAGGTCCTAG AGGCCCACAC CAGGCTGGTC CAGTTTTTCC ACATCCTCCT CCACACCATC
    TCCAGCCGAA ACTTTGAGTC CCTCGTGATC CTGCTGGATT CGGTGGATGA GGTGGACTCT
    GTCCACTGTG CTAGGAGGAT CCCCTGGCTG CCTCCCAAAT GTCCTCCGAG GGTCCACCTC
    ATCGTCTCGG CCTGCTCTGG ACAGCGGGGG GTTTTAGACA CCATTCAGCA GGTGCTCACG
    GACCGAGAGG CTTACTGGGA GGTGAAACCC CTCTCCGGAA ACCAAGGGCA AGAGATGATC
    CAGCTCCTGC TGTCTGCCTC GAGGAGGACA CTGAGTCAGG TGCAGAGGGA CATGCTCTGG
    GCCAGCCTCC CAGAGTGTGG ACACCCAGGG CGGCTGAGGC TGGCTTTCGA GGAGGCCCGG
    AAGTGGGCCT CCTTCACTGT GCCAGCCCCG CTGGCCTCCA CTGCTAAGGA GGCGATGCAA
    CAGCTGTGTG CCCGCCTGGA GCAGACACAC GGGCGGCTCC TGGTGGCCCG CGTGCTGGGC
    TACATTGTGT CTTCCCGGCA GGGGCTCTCA GAGGCGGAGT TAAAGGATGT TCTGTCCTTG
    GATGATGAGG TCCTGCAGAG CGTGTACCAA GACTGGACGC CACCCAGCAA AGAGCTTCTG
    CGCTTCCCAC CCCTGCTGTG GGTGAGGCTT CGTCGAGATC TGGGTAACTG CTTGGCCAGG
    CGGCCCGTGG ACGGCTTCAC CCTTCTGGCC ATTGCTCACA GACAGCTGAC CGAGGTGGTC
    AGAGAGCTCT ACCTGTCTGG GCCTGAGAAA GCCAAGAGTC ACGGAGTCTT GGCCGACTTC
    TTCTCAGGGG CCTGGAGCCA GGGCATCAAG AAACTCATCA CCCTGCCACT CGTGGGAAAA
    CCCCTGAACC TGGACCGCAA GGTGGCCCCT CAGCCCCTGT GGTTCTCCGA CATGGTTGCA
    AACCTGAGGA AGCTGAAGGA GTTACCCTAC CACTTGCTCC ATGCCGGCCG TATCGAGGAG
    CTGAAGCAGG AGGTTCTGGG CAGCATGACC TGGATTTGCT GCCGGGGCCT CTCTGGGGGC
    ATCGAGGGCC TGCTGGAAGA CTTTGACCTG TGCGCCCCAT ACATGGACTG CCCTGAGGTC
    AGCCTGGTCC GGGAAGCTCT CCAGCTGTGC CGCCCGGCAG TGGAGCTCCG AGGCATGGAG
    AGAAGCATCC TGTACACAGA ACTCCTGGCC AGACTCCATT TCTTCAGCAC TTCACATCCC
    GGGCTGGTGG GACAGCTGTG CCAACAGGCA CAGAGCTGGT TCCGGATGTG CCCACAGCCT
    GTGCTGGTGC CCCTTGGAGG ATTCCTCCAG CCCCCTGGAG GGCCTCTGCG GGCAACCCTC
    AGCGGCTGTC ACAAAGGCAT CACTGCCATG GCATGGAGCC TGGAGGAGAA GCTGCTGCTG
    GTTGGCACGC AGGAGGGCAT GGTGGCTGTG TGGGACATGG AGGAACAGCA GGTGATCCAC
    ATCCTTCCTG GACACACAGG AGAGGTAAGG TGTGTGGAAG TGTTTGCCCA AGGGACACAT
    GCTGTCTCTG CCTCGAAAGA CCAGACCTTG CGCTTGTGGG ACTTACTGTC TGGCCAGGAG
    AAATTCACCA TTTGGGATGG AGGTTCAAAA GATCCCACTG AATCTCAGAC CTGGAGCCTT
    CATGTGGATG AAGCAAAGAA GGTTGTGTAT TCAGCTTCAG GCTCAAAGAT CAGTGCTTGG
    AACCTGGAAA CTGCAGAGCT GATTTTCCGT ATCCTGGGGG ATGCCTCTGA CCCCTGGGTA
    TGCATGGCAG TGCTGGCTTC CCAGGCCACA CTGCTGACTG TGTCCCAGGG TGGCGTGATC
    AGTCTGTGGA GCTCAGCCAC GGGAACACTG CACAGGAGGC AACGTTTGTC CAGCGTCAAA
    GAAGATACAC TTGCCTGTGG GGTCTCAGTC CAGAAACGAG GAATGATGGT TACTGGGTCC
    AGCACGGGCT CCATCTCTTT GGTTTCCTCC GAAGGAGACA GCCTGCTGGA AAAGCTTCCG
    GAAGCTGTGG GGTTCCTGGT GGTCTCTGAA GACGAGTCTC TTCTCGCTGC AGGTTTTGGA
    AGGTCCGTGA GGATATTTTT GGCAGATGCA CAGGGATTTC ATCGATTCCT GGCCACTGAC
    CTTGAACATG AAGATGTGGT AGAGACAGCC GTTTTTGGTC CTGAAAACAA TCTGATTGTC
    ACCGGGTCCC GGGATGCACT CATTCAGGTG TGGAGTCTGT CAGCGCAGGG GACTCTGCTG
    GACATCTTGG ACGGTGTCGG GGCCCCTGTG AGCCTGCTGG CCCGGGGTGG GGCCTTGGTG
    GCATCTGCTT CCCGTCAGTC CTCATCTTTT AAAGTCTGGG ATCTCACCTG TGCTCAGAAG
    CCGAGGGCCT CTAACCCATT CTTGGACCGC ACTGGCCTCA CAGCAGTGTC ACACAACGGC
    AGCTACGTCT ATTTCCCTAA AACTGGGGAC AAAAACAAAG TCAGCATTTG GGACTTGGCA
    GAAGGTGAGG AGCAAGATGC TCTGGACACC TCCAACGAGG TCAGGTGTCT GGAGGTTGCC
    GAGCAGGCCA ATCTCCTTTT CACTGGCCTC GTTTCGGGGA TTGTCCTGGT GTTCCCCCTG
    AACTCCAGAC AAGACGTGAT GTGTATCCCT CCTCCAGAAG CCCGGAAAGC CGTCAACTGC
    ATGGCTCTTA GTAAGGACGA GGGGCACCTG GCCATTGCCT ATGACAGCAC CGTCTTGGTG
    CTGGACATCA GCCCGGGGGA CCCCTGTCCA GTCATCGACG GGCCAATATA CACCTTCTAT
    ACCCAACTGC CTGACACCAT AGCCAGTGTG GCCATTCTGG CTGACTGTCG TGTCATCTAC
    GGCATGATCA ACGGTGACCT CTTCCTTTAC GAGTGTGTGA ATTCCAAGGT GTTCCCTCTG
    GAGGCCCATG GGAGCCGAGT CACCTGTGTG GAGGTCAGCC ACAAAGAGCA GCTGGCAGTC
    AGCGGGGCCG AGGAAGCCCT ACTGTGTCTC TGGGACCTGC AGGTGTGCAA GTGGAAATTC
    GAGATGAGCT ACACGAATTC TTACTGTATA GGAGTCCAAT GTGCTTGCTT CTCCAAAGAT
    GACAAGTATG TCTACGCAGG CTTGAAAGAT TGCTCCATAA TTGTATGGAG TGTGCTGGAT
    GGCACACTGC TGGCTGTCCA GTTTGTGCAT GCCGTGGTGA ACAGAATCAT CCCAACCTCC
    AGTGGCTTCA TTGCCCCCTC CAGACACGGC TATCTCATCC GTGAGCGTTT CCAGTGCCCT
    TCATCAGGAA TCTCACAACA GGACCCTCTC AAGAACTTCA AGAAAGCAGT GTGGATGGTC
    AAATCCAGGC AGAGAGAAGA GCTGGCAGCT GCTGCAGGAG CACTCCAGGA CTTGAGATCG
    GAGAGTGCCC AGGGAAATGA ATCCAGATCA AACAAACGCT CACAAGTCTG CCTGCTGGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327374.1
    CDS16..4638
    Translation

    Target ORF information:

    RefSeq Version XM_015471888.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471888.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTCGAGG TGGTTGACCT GAGGTGGGGT ATTCGGAACA CTGAGGCCAC TAACCACATG 
    ACCACAGAAC TCTGCTTGGA GGAGCTTGAC CGGTGTCGGA AAACATCCAT AGGGCCAGCT
    TTTGTTGCCC TCGTGGGTGA CCAGTACGGC CCCTGCCCCG TCCCCACGCA GATCGAGGAA
    AAGGAGTGGG AGGCGCTGAA GGCTCAGCTG ACCGCCAGGC CCCGCGACCT GGAGCTGGTG
    GCACGATGCT TCTGGAGAGA CGAGAACGCG GTGCCCCCCG CCTACCTGCT GCAGGCCCTG
    GGCACTGGGG AGGCCTCTGG GCCTGAAGAG GCCACCCTGC TCTCTGCACT GCGCTCCGGA
    GCCCAGGAGG CCCGCAGGCT GGGGCTCATC AGCCAGGAGC GGTGGTACTG CTACCACCGG
    TCAGTCATTG AGTGGGAAAT AGAACAGGCC TTGCTGAGCT CAACGGTTGG GGATCACGGG
    GCAACAGTCT TCCTGAGAGA GACCCAAGAC CTCAATAAAC ACATCCTAGA AGACTGTGCC
    CTCAGAATGG TGGACCGTCT TGCAGATGGT TGCCTGGACA CGGATGCCCA GCACCTTCTC
    AGCAACCTCA AGGGGCGCAT CGCTGACCGG CAGCCTGGAG TTCTCAAGGC CCACCACCTA
    GCGTGGAGCC GAGACCTGGT GAACCCCAAA AACAAGGCTC ATGCCCGATA CCTGAAGGAG
    CTGGGGGAGC AGTTTGTGGC CAGGGCCAAC CATCAGGTTC TTGAGTGCCT CCGGGAGCTG
    GAGGCAGGCA GGCAGGAGTT GGCATGGCTC TACCAGGAGA TCCGCCACCA CCTGGGACTC
    AGTGCCGAGG TCAACCGGAC CTTCTGTGGG CGCCAGGAGC TCCTGACCCA GCTGGGCCAG
    CGACTGCGAC AGAATGATGG CCACCCCCAC CCACCCCTGG TGCTTTTTGG ACCCCCAGGG
    ATTGGAAAGA CGGCTCTGAT GTGCAAGCTG GCTGAGCAAA TGTCGGGGCT GCTCGGCCGC
    AAGACAGTGA CCATTCTGCG GCTTCTGGGG ACGTCACAGA TGAGCTCTGA GGCCCGAGGC
    CTGCTCCAGA GCATCTGCTT CCAGGTGTGC CTGGCCTACG GGCTGCCCCT GCCCCCTGCC
    CAGGTCCTAG AGGCCCACAC CAGGCTGGTC CAGTTTTTCC ACATCCTCCT CCACACCATC
    TCCAGCCGAA ACTTTGAGTC CCTCGTGATC CTGCTGGATT CGGTGGATGA GGTGGACTCT
    GTCCACTGTG CTAGGAGGAT CCCCTGGCTG CCTCCCAAAT GTCCTCCGAG GGTCCACCTC
    ATCGTCTCGG CCTGCTCTGG ACAGCGGGGG GTTTTAGACA CCATTCAGCA GGTGCTCACG
    GACCGAGAGG CTTACTGGGA GGTGAAACCC CTCTCCGGAA ACCAAGGGCA AGAGATGATC
    CAGCTCCTGC TGTCTGCCTC GAGGAGGACA CTGAGTCAGG TGCAGAGGGA CATGCTCTGG
    GCCAGCCTCC CAGAGTGTGG ACACCCAGGG CGGCTGAGGC TGGCTTTCGA GGAGGCCCGG
    AAGTGGGCCT CCTTCACTGT GCCAGCCCCG CTGGCCTCCA CTGCTAAGGA GGCGATGCAA
    CAGCTGTGTG CCCGCCTGGA GCAGACACAC GGGCGGCTCC TGGTGGCCCG CGTGCTGGGC
    TACATTGTGT CTTCCCGGCA GGGGCTCTCA GAGGCGGAGT TAAAGGATGT TCTGTCCTTG
    GATGATGAGG TCCTGCAGAG CGTGTACCAA GACTGGACGC CACCCAGCAA AGAGCTTCTG
    CGCTTCCCAC CCCTGCTGTG GGTGAGGCTT CGTCGAGATC TGGGTAACTG CTTGGCCAGG
    CGGCCCGTGG ACGGCTTCAC CCTTCTGGCC ATTGCTCACA GACAGCTGAC CGAGGTGGTC
    AGAGAGCTCT ACCTGTCTGG GCCTGAGAAA GCCAAGAGTC ACGGAGTCTT GGCCGACTTC
    TTCTCAGGGG CCTGGAGCCA GGGCATCAAG AAACTCATCA CCCTGCCACT CGTGGGAAAA
    CCCCTGAACC TGGACCGCAA GGTGGCCCCT CAGCCCCTGT GGTTCTCCGA CATGGTTGCA
    AACCTGAGGA AGCTGAAGGA GTTACCCTAC CACTTGCTCC ATGCCGGCCG TATCGAGGAG
    CTGAAGCAGG AGGTTCTGGG CAGCATGACC TGGATTTGCT GCCGGGGCCT CTCTGGGGGC
    ATCGAGGGCC TGCTGGAAGA CTTTGACCTG TGCGCCCCAT ACATGGACTG CCCTGAGGTC
    AGCCTGGTCC GGGAAGCTCT CCAGCTGTGC CGCCCGGCAG TGGAGCTCCG AGGCATGGAG
    AGAAGCATCC TGTACACAGA ACTCCTGGCC AGACTCCATT TCTTCAGCAC TTCACATCCC
    GGGCTGGTGG GACAGCTGTG CCAACAGGCA CAGAGCTGGT TCCGGATGTG CCCACAGCCT
    GTGCTGGTGC CCCTTGGAGG ATTCCTCCAG CCCCCTGGAG GGCCTCTGCG GGCAACCCTC
    AGCGGCTGTC ACAAAGGCAT CACTGCCATG GCATGGAGCC TGGAGGAGAA GCTGCTGCTG
    GTTGGCACGC AGGAGGGCAT GGTGGCTGTG TGGGACATGG AGGAACAGCA GGTGATCCAC
    ATCCTTCCTG GACACACAGG AGAGGTAAGG TGTGTGGAAG TGTTTGCCCA AGGGACACAT
    GCTGTCTCTG CCTCGAAAGA CCAGACCTTG CGCTTGTGGG ACTTACTGTC TGGCCAGGAG
    AAATTCACCA TTTGGGATGG AGGTTCAAAA GATCCCACTG AATCTCAGAC CTGGAGCCTT
    CATGTGGATG AAGCAAAGAA GGTTGTGTAT TCAGCTTCAG GCTCAAAGAT CAGTGCTTGG
    AACCTGGAAA CTGCAGAGCT GATTTTCCGT ATCCTGGGGG ATGCCTCTGA CCCCTGGGTA
    TGCATGGCAG TGCTGGCTTC CCAGGCCACA CTGCTGACTG TGTCCCAGGG TGGCGTGATC
    AGTCTGTGGA GCTCAGCCAC GGGAACACTG CACAGGAGGC AACGTTTGTC CAGCGTCAAA
    GAAGATACAC TTGCCTGTGG GGTCTCAGTC CAGAAACGAG GAATGATGGT TACTGGGTCC
    AGCACGGGCT CCATCTCTTT GGTTTCCTCC GAAGGAGACA GCCTGCTGGA AAAGCTTCCG
    GAAGCTGTGG GGTTCCTGGT GGTCTCTGAA GACGAGTCTC TTCTCGCTGC AGGTTTTGGA
    AGGTCCGTGA GGATATTTTT GGCAGATGCA CAGGGATTTC ATCGATTCCT GGCCACTGAC
    CTTGAACATG AAGATGTGGT AGAGACAGCC GTTTTTGGTC CTGAAAACAA TCTGATTGTC
    ACCGGGTCCC GGGATGCACT CATTCAGGTG TGGAGTCTGT CAGCGCAGGG GACTCTGCTG
    GACATCTTGG ACGGTGTCGG GGCCCCTGTG AGCCTGCTGG CCCGGGGTGG GGCCTTGGTG
    GCATCTGCTT CCCGTCAGTC CTCATCTTTT AAAGTCTGGG ATCTCACCTG TGCTCAGAAG
    CCGAGGGCCT CTAACCCATT CTTGGACCGC ACTGGCCTCA CAGCAGTGTC ACACAACGGC
    AGCTACGTCT ATTTCCCTAA AACTGGGGAC AAAAACAAAG TCAGCATTTG GGACTTGGCA
    GAAGGTGAGG AGCAAGATGC TCTGGACACC TCCAACGAGG TCAGGTGTCT GGAGGTTGCC
    GAGCAGGCCA ATCTCCTTTT CACTGGCCTC GTTTCGGGGA TTGTCCTGGT GTTCCCCCTG
    AACTCCAGAC AAGACGTGAT GTGTATCCCT CCTCCAGAAG CCCGGAAAGC CGTCAACTGC
    ATGGCTCTTA GTAAGGACGA GGGGCACCTG GCCATTGCCT ATGACAGCAC CGTCTTGGTG
    CTGGACATCA GCCCGGGGGA CCCCTGTCCA GTCATCGACG GGCCAATATA CACCTTCTAT
    ACCCAACTGC CTGACACCAT AGCCAGTGTG GCCATTCTGG CTGACTGTCG TGTCATCTAC
    GGCATGATCA ACGGTGACCT CTTCCTTTAC GAGTGTGTGA ATTCCAAGGT GTTCCCTCTG
    GAGGCCCATG GGAGCCGAGT CACCTGTGTG GAGGTCAGCC ACAAAGAGCA GCTGGCAGTC
    AGCGGGGCCG AGGAAGCCCT ACTGTGTCTC TGGGACCTGC AGGTGTGCAA GTGGAAATTC
    GAGATGAGCT ACACGAATTC TTACTGTATA GGAGTCCAAT GTGCTTGCTT CTCCAAAGAT
    GACAAGTATG TCTACGCAGG CTTGAAAGAT TGCTCCATAA TTGTATGGAG TGTGCTGGAT
    GGCACACTGC TGGCTGTCCA GTTTGTGCAT GCCGTGGTGA ACAGAATCAT CCCAACCTCC
    AGTGGCTTCA TTGCCCCCTC CAGACACGGC TATCTCATCC GTGAGCGTTT CCAGTGCCCT
    TCATCAGGAA TCTCACAACA GGACCCTCTC AAGAACTTCA AGAAAGCAGT GTGGATGGTC
    AAATCCAGGC AGAGAGAAGA GCTGGCAGCT GCTGCAGGAG CACTCCAGGA CTTGAGATCG
    GAGAGTGCCC AGGGAAATGA ATCCAGATCA AACAAACGCT CACAAGTCTG CCTGCTGGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38635
    Clone ID Related Accession (Same CDS sequence) NM_001102210.1
    Accession Version NM_001102210.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product NACHT domain- and WD repeat-containing protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151394.1. On Aug 21, 2007 this sequence version replaced XM_618189.3. ##Evidence-Data-START## Transcript exon combination :: BC151394.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145422 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTGGACC GTCTTGCAGA TGGTTGCCTG GACACGGATG CCCAGAACCT TCTCAGCAAC 
    CTCAAGGGGC GCATCGCTGA CCGGCAGCCT GGAGTTCTCA AGGCCCACCA CCTAGCGTGG
    AGCCGAGACC TGGTGAACCC CAAAAACAAG GCTCATGCCC GATACCTGAA GGAGCTGGGG
    GAGCAGTTTG TGGCCAGGGC CAACCATCAG GTTCTTGAGT GCCTCCGGGA GCTGGAGGCA
    GGCAGGCAGG AGTTGGCATG GCTCTACCAG GAGATCCGCC ACCACCTGGG ACTCAGTGCC
    GAGGTCAACC GGACCTTCTG TGGGCGCCAG GAGCTCCTGA CCCAGCTGGG CCAGCGACTG
    CGACAGAATG ATGGCCACCC CCACCCACCC CTGGTGCTTT TTGGACCCCC AGGGATTGGA
    AAGACGGCTC TGATGTGCAA GCTGGCTGAG CAAATGTCGG GGCTGCTCGG CCGCAAGACA
    GTGACCATTC TGCGGCTTCT GGGGACGTCA CAGATGAGCT CTGAGGCCCG AGGCCTGCTC
    CAGAGCATCT GCTTCCAGGT GTGCCTGGCC TACGGGCTGC CCCTGCCCCC TGCCCAGGTC
    CTAGAGGCCC ACACCAGGCT GGTCCAGTTT TTCCACATCC TCCTCCACAC CATCTCCAGC
    CGAAACTTTG AGTCCCTCGT GATCCTGCTG GATTCGGTGG ATGAGGTGGA CTCTGTCCAC
    TGTGCTAGGA GGATCCCCTG GCTGCCTCCC AAATGTCCTC CGAGGGTCCA CCTCATCGTC
    TCGGCCTGCT CTGGACAGCG GGGGGTTTTA GACACCATTC AGCAGGTGCT CACGGACCGA
    GAGGCTTACT GGGAGGTGAA ACCCCTCTCC GGAAACCAAG GGCAAGAGAT GATCCAGCTC
    CTGCTGTCTG CCTCGAGGAG GACACTGAGT CAGGTGCAGA GGGACATGCT CTGGGCCAGC
    CTCCCAGAGT GTGGACACCC AGGGCGGCTG AGGCTGGCTT TCGAGGAGGC CCGGAAGTGG
    GCCTCCTTCA CTGTGCCAGC CCCGCTGGCC TCCACTGCTA AGGAGGCGAT GCAACAGCTG
    TGTGCCCGCC TGGAGCAGAC ACACGGGCGG CTCCTGGTGG CCCGCGTGCT GGGCTACATT
    GTGTCTTCCC GGCAGGGGCT CTCAGAGGCG GAGTTAAAGG ATGTTCTGTC CTTGGATGAT
    GAGGTCCTGC AGAGCGTGTA CCAAGACTGG ACCCCACCCA GCAAAGAGCT TCTGCGCTTC
    CCACCCCTGC TGTGGGTGAG GCTTCGTCGA GATCTGGGTA ACTGCTTGGC CAGGCGGCCC
    GTGGACGGCT TCACCCTTCT GGCCATTGCT CACAGACAGC TGACCGAGGT GGTCAGAGAG
    CTCTACCTGT CTGGGCCTGA GAAAGCCAAG AGTCACGGAG TCTTGGCCGA CTTCTTCTCA
    GGGGCCTGGA GCCAGGGCAT CAAGAAACTC ATCACCCTGC CACTCGTGGG AAAACCCCTG
    AACCTGGACC GCAAGGTGGC CCCTCAGCCC CTGTGGTTCT CCGACATGGT TGCAAACCTG
    AGGAAGCTGA AGGAGTTACC CTACCACTTG CTCCATGCCG GCCGTATCGA GGAGCTGAAG
    CAGGAGGTTC TGGGCAGCAT GACCTGGATT TGCTGCCGGG GCCTCTCTGG GGGCATCGAG
    GGCCTGCTGG AAGACTTTGA CCTGTGCGCC CCATACATGG ACTGCCCTGA GGTCAGCCTG
    GTCCGGGAAG CTCTCCAGCT GTGCCGCCCG GCAGTGGAGC TCCGAGGCAT GGAGAGAAGC
    ATCCTGTACA CAGAACTCCT GGCCAGACTC CATTTCTTCA GCACTTCACA TCCCGGGCTG
    GTGGGACAGC TGTGCCAACA GGCACAGAGC TGGTTCCGGA TGTGCCCACA GCCTGTGCTG
    GTGCCCCTTG GAGGATTCCT CCAGCCCCCT GGAGGGCCTC TGCGGGCAAC CCTCAGCGGC
    TGTCACAAAG GCATCACTGC CATGGCATGG AGCCTGGAGG AGAAGCTGCT GCTGGTTGGC
    ACGCAGGAGG GCATGGTGGC TGTGTGGGAC ATGGAGGAAC AGCAGGTGAT CCACATCCTT
    CCTGGACACA CAGGAGAGGT AAGGTGTGTG GAAGTGTTTG CCCAAGGGAC ACATGCTGTC
    TCTGCCTCGA AAGACCAGAC CTTGCGCTTG TGGGACTTAC TGTCTGGCCA GGAGAAATTC
    ACCATTTGGG ATGGAGGTTC AAAAGATCCC ACTGAATCTC AGACCTGGAG CCTTCATGTG
    GATGAAGCAA AGAAGGTTGT GTATTCAGCT TCAGGCTCAA AGATCAGTGC TTGGAACCTG
    GAAACTGCAG AGCTGATTTT CCGTATCCTG GGGGATGCCT CTGACCCCTG GGTATGCATG
    GCAGTGCTGG CTTCCCAGGC CACACTGCTG ACTGTGTCCC AGGGTGGCGT GATCAGTCTG
    TGGAGCTCAG CCACGGGAAC ACTGCACAGG AGGCAACGTT TGTCCAGCGT CAAAGAAGAT
    ACACTTGCCT GTGGGGTCTC AGTCCAGAAA CGAGGAATGA TGGTTACTGG GTCCAGCACG
    GGCTCCATCT CTTTGGTTTC CTCCGAAGGA GACAGCCTGC TGGAAAAGCT TCCGGAAGCT
    GTGGGGTTCC TGGTGGTCTC TGAAGACGAG TCTCTTCTCG CTGCAGGTTT TGGAAGGTCC
    GTGAGGATAT TTTTGGCAGA TGCACAGGGA TTTCATCGAT TCCTGGCCAC TGACCTTGAA
    CATGAAGATG TGGTAGAGAC AGCCGTTTTT GGTCCTGAAA ACAATCTGAT TGTCACCGGG
    TCCCGGGATG CACTCATTCA GGTGTGGAGT CTGTCAGCGC AGGGGACTCT GCTGGACATC
    TTGGACGGTG TCGGGGCCCC TGTGAGCCTG CTGGCCCGGG GTGGGGCCTT GGTGGCATCT
    GCTTCCCGTC AGTCCTCATC TTTTAAAGTC TGGGATCTCA CCTGTGCTCA GAAGCCGAGG
    GCCTCTAACC CATTCTTGGA CCGCACTGGC CTCACAGCAG TGTCACACAA CGGCAGCTAC
    GTCTATTTCC CTAAAACTGG GGACAAAAAC AAAGTCAGCA TTTGGGACTT GGCAGAAGGT
    GAGGAGCAAG ATGCTCTGGA CACCTCCAAC GAGGTCAGGT GTCTGGAGGT TGCCGAGCAG
    GCCAATCTCC TTTTCACTGG CCTCGTTTCG GGGATTGTCC TGGTGTTCCC CCTGAACTCC
    AGACAAGACG TGATGTGTAT CCCTCCTCCA GAAGCCCGGA AAGCCGTCAA CTGCATGGCT
    CTTAGTAAGG ACGAGGGGCA CCTGGCCATT GCCTATGACA GCACCGTCTT GGTGCTGGAC
    ATCAGCCCGG GGGACCCCTG TCCAGTCATC GACGGGCCAA TATACACCTT CTATACCCAA
    CTGCCTGACA CCATAGCCAG TGTGGCCATT CTGGCTGACT GTCGTGTCAT CTACGGCATG
    ATCAACGGTG ACCTCTTCCT TTACGAGTGT GTGAATTCCA AGGTGTTCCC TCTGGAGGCC
    CATGGGAGCC GAGTCACCTG TGTGGAGGTC AGCCACAAAG AGCAGCTGGC AGTCAGCGGG
    GCCGAGGAAG CCCTACTGTG TCTCTGGGAC CTGCAGGTGT GCAAGTGGAA ATTCGAGATG
    AGCTACACGA ATTCTTACTG TATAGGAGTC CAATGTGCTT GCTTCTCCAA AGATGACAAG
    TATGTCTACG CAGGCTTGAA AGATTGCTCC ATAATTGTAT GGAGTGTGCT GGATGGCACA
    CTGCTGGCTG TCCAGTTTGT GCATGCCGTG GTGAACAGAA TCATCCCAAC CTCCAGTGGC
    TTCATTGCCC CCTCCAGACA CGGCTATCTC ATCCGTGAGC GTTTCCAGTG CCCTTCATCA
    GGAATCTCAC AACAGGACCC TCTCAAGAAC TTCAAGAAAG CAGTGTGGAT GGTCAAATCC
    AGGCAGAGAG AAGAGCTGGC AGCTGCTGCA GGAGCACTCC AGGACTTGAG ATCGGAGAGT
    GCCCAGGGAA ATGAATCCAG ATCAAACAAA CGCTCACAAG TCTGCCTGCT GGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001095680.1
    CDS16..4092
    Translation

    Target ORF information:

    RefSeq Version NM_001102210.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NACHT and WD repeat domain containing 1 (NWD1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102210.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTGGACC GTCTTGCAGA TGGTTGCCTG GACACGGATG CCCAGAACCT TCTCAGCAAC 
    CTCAAGGGGC GCATCGCTGA CCGGCAGCCT GGAGTTCTCA AGGCCCACCA CCTAGCGTGG
    AGCCGAGACC TGGTGAACCC CAAAAACAAG GCTCATGCCC GATACCTGAA GGAGCTGGGG
    GAGCAGTTTG TGGCCAGGGC CAACCATCAG GTTCTTGAGT GCCTCCGGGA GCTGGAGGCA
    GGCAGGCAGG AGTTGGCATG GCTCTACCAG GAGATCCGCC ACCACCTGGG ACTCAGTGCC
    GAGGTCAACC GGACCTTCTG TGGGCGCCAG GAGCTCCTGA CCCAGCTGGG CCAGCGACTG
    CGACAGAATG ATGGCCACCC CCACCCACCC CTGGTGCTTT TTGGACCCCC AGGGATTGGA
    AAGACGGCTC TGATGTGCAA GCTGGCTGAG CAAATGTCGG GGCTGCTCGG CCGCAAGACA
    GTGACCATTC TGCGGCTTCT GGGGACGTCA CAGATGAGCT CTGAGGCCCG AGGCCTGCTC
    CAGAGCATCT GCTTCCAGGT GTGCCTGGCC TACGGGCTGC CCCTGCCCCC TGCCCAGGTC
    CTAGAGGCCC ACACCAGGCT GGTCCAGTTT TTCCACATCC TCCTCCACAC CATCTCCAGC
    CGAAACTTTG AGTCCCTCGT GATCCTGCTG GATTCGGTGG ATGAGGTGGA CTCTGTCCAC
    TGTGCTAGGA GGATCCCCTG GCTGCCTCCC AAATGTCCTC CGAGGGTCCA CCTCATCGTC
    TCGGCCTGCT CTGGACAGCG GGGGGTTTTA GACACCATTC AGCAGGTGCT CACGGACCGA
    GAGGCTTACT GGGAGGTGAA ACCCCTCTCC GGAAACCAAG GGCAAGAGAT GATCCAGCTC
    CTGCTGTCTG CCTCGAGGAG GACACTGAGT CAGGTGCAGA GGGACATGCT CTGGGCCAGC
    CTCCCAGAGT GTGGACACCC AGGGCGGCTG AGGCTGGCTT TCGAGGAGGC CCGGAAGTGG
    GCCTCCTTCA CTGTGCCAGC CCCGCTGGCC TCCACTGCTA AGGAGGCGAT GCAACAGCTG
    TGTGCCCGCC TGGAGCAGAC ACACGGGCGG CTCCTGGTGG CCCGCGTGCT GGGCTACATT
    GTGTCTTCCC GGCAGGGGCT CTCAGAGGCG GAGTTAAAGG ATGTTCTGTC CTTGGATGAT
    GAGGTCCTGC AGAGCGTGTA CCAAGACTGG ACCCCACCCA GCAAAGAGCT TCTGCGCTTC
    CCACCCCTGC TGTGGGTGAG GCTTCGTCGA GATCTGGGTA ACTGCTTGGC CAGGCGGCCC
    GTGGACGGCT TCACCCTTCT GGCCATTGCT CACAGACAGC TGACCGAGGT GGTCAGAGAG
    CTCTACCTGT CTGGGCCTGA GAAAGCCAAG AGTCACGGAG TCTTGGCCGA CTTCTTCTCA
    GGGGCCTGGA GCCAGGGCAT CAAGAAACTC ATCACCCTGC CACTCGTGGG AAAACCCCTG
    AACCTGGACC GCAAGGTGGC CCCTCAGCCC CTGTGGTTCT CCGACATGGT TGCAAACCTG
    AGGAAGCTGA AGGAGTTACC CTACCACTTG CTCCATGCCG GCCGTATCGA GGAGCTGAAG
    CAGGAGGTTC TGGGCAGCAT GACCTGGATT TGCTGCCGGG GCCTCTCTGG GGGCATCGAG
    GGCCTGCTGG AAGACTTTGA CCTGTGCGCC CCATACATGG ACTGCCCTGA GGTCAGCCTG
    GTCCGGGAAG CTCTCCAGCT GTGCCGCCCG GCAGTGGAGC TCCGAGGCAT GGAGAGAAGC
    ATCCTGTACA CAGAACTCCT GGCCAGACTC CATTTCTTCA GCACTTCACA TCCCGGGCTG
    GTGGGACAGC TGTGCCAACA GGCACAGAGC TGGTTCCGGA TGTGCCCACA GCCTGTGCTG
    GTGCCCCTTG GAGGATTCCT CCAGCCCCCT GGAGGGCCTC TGCGGGCAAC CCTCAGCGGC
    TGTCACAAAG GCATCACTGC CATGGCATGG AGCCTGGAGG AGAAGCTGCT GCTGGTTGGC
    ACGCAGGAGG GCATGGTGGC TGTGTGGGAC ATGGAGGAAC AGCAGGTGAT CCACATCCTT
    CCTGGACACA CAGGAGAGGT AAGGTGTGTG GAAGTGTTTG CCCAAGGGAC ACATGCTGTC
    TCTGCCTCGA AAGACCAGAC CTTGCGCTTG TGGGACTTAC TGTCTGGCCA GGAGAAATTC
    ACCATTTGGG ATGGAGGTTC AAAAGATCCC ACTGAATCTC AGACCTGGAG CCTTCATGTG
    GATGAAGCAA AGAAGGTTGT GTATTCAGCT TCAGGCTCAA AGATCAGTGC TTGGAACCTG
    GAAACTGCAG AGCTGATTTT CCGTATCCTG GGGGATGCCT CTGACCCCTG GGTATGCATG
    GCAGTGCTGG CTTCCCAGGC CACACTGCTG ACTGTGTCCC AGGGTGGCGT GATCAGTCTG
    TGGAGCTCAG CCACGGGAAC ACTGCACAGG AGGCAACGTT TGTCCAGCGT CAAAGAAGAT
    ACACTTGCCT GTGGGGTCTC AGTCCAGAAA CGAGGAATGA TGGTTACTGG GTCCAGCACG
    GGCTCCATCT CTTTGGTTTC CTCCGAAGGA GACAGCCTGC TGGAAAAGCT TCCGGAAGCT
    GTGGGGTTCC TGGTGGTCTC TGAAGACGAG TCTCTTCTCG CTGCAGGTTT TGGAAGGTCC
    GTGAGGATAT TTTTGGCAGA TGCACAGGGA TTTCATCGAT TCCTGGCCAC TGACCTTGAA
    CATGAAGATG TGGTAGAGAC AGCCGTTTTT GGTCCTGAAA ACAATCTGAT TGTCACCGGG
    TCCCGGGATG CACTCATTCA GGTGTGGAGT CTGTCAGCGC AGGGGACTCT GCTGGACATC
    TTGGACGGTG TCGGGGCCCC TGTGAGCCTG CTGGCCCGGG GTGGGGCCTT GGTGGCATCT
    GCTTCCCGTC AGTCCTCATC TTTTAAAGTC TGGGATCTCA CCTGTGCTCA GAAGCCGAGG
    GCCTCTAACC CATTCTTGGA CCGCACTGGC CTCACAGCAG TGTCACACAA CGGCAGCTAC
    GTCTATTTCC CTAAAACTGG GGACAAAAAC AAAGTCAGCA TTTGGGACTT GGCAGAAGGT
    GAGGAGCAAG ATGCTCTGGA CACCTCCAAC GAGGTCAGGT GTCTGGAGGT TGCCGAGCAG
    GCCAATCTCC TTTTCACTGG CCTCGTTTCG GGGATTGTCC TGGTGTTCCC CCTGAACTCC
    AGACAAGACG TGATGTGTAT CCCTCCTCCA GAAGCCCGGA AAGCCGTCAA CTGCATGGCT
    CTTAGTAAGG ACGAGGGGCA CCTGGCCATT GCCTATGACA GCACCGTCTT GGTGCTGGAC
    ATCAGCCCGG GGGACCCCTG TCCAGTCATC GACGGGCCAA TATACACCTT CTATACCCAA
    CTGCCTGACA CCATAGCCAG TGTGGCCATT CTGGCTGACT GTCGTGTCAT CTACGGCATG
    ATCAACGGTG ACCTCTTCCT TTACGAGTGT GTGAATTCCA AGGTGTTCCC TCTGGAGGCC
    CATGGGAGCC GAGTCACCTG TGTGGAGGTC AGCCACAAAG AGCAGCTGGC AGTCAGCGGG
    GCCGAGGAAG CCCTACTGTG TCTCTGGGAC CTGCAGGTGT GCAAGTGGAA ATTCGAGATG
    AGCTACACGA ATTCTTACTG TATAGGAGTC CAATGTGCTT GCTTCTCCAA AGATGACAAG
    TATGTCTACG CAGGCTTGAA AGATTGCTCC ATAATTGTAT GGAGTGTGCT GGATGGCACA
    CTGCTGGCTG TCCAGTTTGT GCATGCCGTG GTGAACAGAA TCATCCCAAC CTCCAGTGGC
    TTCATTGCCC CCTCCAGACA CGGCTATCTC ATCCGTGAGC GTTTCCAGTG CCCTTCATCA
    GGAATCTCAC AACAGGACCC TCTCAAGAAC TTCAAGAAAG CAGTGTGGAT GGTCAAATCC
    AGGCAGAGAG AAGAGCTGGC AGCTGCTGCA GGAGCACTCC AGGACTTGAG ATCGGAGAGT
    GCCCAGGGAA ATGAATCCAG ATCAAACAAA CGCTCACAAG TCTGCCTGCT GGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.