Nwd1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006531091.3	XP_006531154.1	NACHT domain- and WD repeat-containing protein 1 isoform X5
XM_006531092.2	XP_006531155.1	NACHT domain- and WD repeat-containing protein 1 isoform X6
XM_006531087.4	XP_006531150.1	NACHT domain- and WD repeat-containing protein 1 isoform X2
XM_030243605.1	XP_030099465.1	NACHT domain- and WD repeat-containing protein 1 isoform X4
XM_006531088.3	XP_006531151.1	NACHT domain- and WD repeat-containing protein 1 isoform X5
XM_006531090.2	XP_006531153.1	NACHT domain- and WD repeat-containing protein 1 isoform X3
XM_030243607.1	XP_030099467.1	NACHT domain- and WD repeat-containing protein 1 isoform X6
XM_006531093.2	XP_006531156.1	NACHT domain- and WD repeat-containing protein 1 isoform X7
XM_006531089.2	XP_006531152.1	NACHT domain- and WD repeat-containing protein 1 isoform X5
XM_030243606.1	XP_030099466.1	NACHT domain- and WD repeat-containing protein 1 isoform X6
NM_176940.5	NP_795914.3	NACHT domain- and WD repeat-containing protein 1
XM_006531087.3	XP_006531150.1	NACHT domain- and WD repeat-containing protein 1 isoform X2
XM_006531086.2	XP_006531149.1	NACHT domain- and WD repeat-containing protein 1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu89868	XM_030243605.1 Latest version!	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu89869	XM_030243606.1 Latest version!	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu89869	XM_030243607.1 Latest version!	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu41534	XM_006531091.3 Latest version!	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu41535	XM_006531092.2 Latest version!	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu41536	XM_006531093.2 Latest version!	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu41531	XM_006531087.4 Latest version!	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu05763	NM_176940.5 Latest version!	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu41530	XM_006531086.2 Latest version!	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu41532	XM_006531089.2 Latest version!	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu41532	XM_006531088.3 Latest version!	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu41533	XM_006531090.2 Latest version!	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu41531	XM_006531087.3	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

Nwd1 ( XM_030243605.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099465.1 Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu89868
Clone ID Related Accession (Same CDS sequence)	XM_030243605.1
Accession Version	XM_030243605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG GATTTGGACA GAACCTGGAT TTTGTGGAGG 
TACGGTCTCT CAGAAGCAGA GCTGAAGGAT GTGTTGTCTT TGGATGATGA AGTCTTGCAG 
GCTGTGTACC GGGACTGGAC GCCACCTAGC AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG 
TGGGTGCGGC TCCGCCGGGA CCTGGGACAC TGCCTAGTCA GGAGGCCCGT GGATGGCTGC 
ATGCTACTGG CCATTGCACA CAGACAGCTG TCCCAGGTAA TCCAAGTTCG ATATCTGTCT 
GGGCCTGAGA GAGCCAAGAG GCATGGCGTG CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC 
CAGGGGATCA AGAAACTTAT CACCCTGCCG CTTGTAGGGA AGCCCCTGAA CCTGGACCGC 
AAGGTGGCCC CACAGCCACT GTGGTTCTCA AGCACAGTGG CCAACCTGAG GAAGCTGACC 
GAACTGCCCT TCCATCTGCT GCATGCAGGC CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG 
GGGAACATGA GTTGGATCTC ATGTCGGGGC ATCTCTGGAG GCATTGAGGT CCTGCTGGAT 
GATTTTGACA TGTGTGCCCC TCACATGAAC TCTCCTGAGG TTGATCTGGT TAGAGAAGCC 
ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC CGGGGCCTGG AGAAGAGCGT CCTATACACC 
GAGCTCCTGG CCAGACTCCT TTTCTTTGCT GCATCACATC CAGCAATGAT CGGACAACTG 
TGCCAACAAG CCCAGAGCTG GTTCCGTGCC TGCCCCTACC CAATGCTGGT GCCCCTGGCA 
GGATTCCTGC AGCCACCTGG TGGACCTCTT CGAGCCACTC TTACTGGGTG TCACAAAGGC 
ATCACCGCCA TAGCATGGAG CCTGGAGGAG AAACTCCTGG TGGTGGGCAC CCAGGATGGT 
GCCATGGTCG TGTGGGACGT GGAGGAACAA CAGGTGGTAC ACGTTCTAAT GGGACACACA 
GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC CAAGGGACTC TTGCCATCTC TGCATCAAAG 
GACCATACCC TGCGTCTGTG GAGTCTGCTT TCCGGCCAGG AGAAAGTCAC CATTTTGGAT 
GGAGGGTCAC AAAATCCCAC AGAGCCCCAG AGTTGGGACC TTCATGTGGA TGAGAGAAAC 
AATGTTGTAT ACTCGACATC TGGTGCAAGG ATCAACATGT GGAATCTGGA AACTTCGAAG 
CTGGTTTTCT GTATCACAGG AGATGTGTCT GATCCCTGGG TCTGTGTGGC TTTGTTGGCT 
GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC 
ATGGGAAAAC TGCAGGAGAA ACACCAGTTA TCCAGCATCA AAGAAGAGAC ACCCACCTGT 
GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG GTCGCTGGCT TCAGCAGTGG CTCCATTGCC 
CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG 
GTGGTTTCTG AAGACGATTC CCTTCTTGTG GCAGGCTTTG GAAGATTTGT GCGGATTTTC 
TTGGCTGACT CGCAAGGCTT CCACCGGTTC ATGGCTTCGG ATCTGGAACA CGAGGACATG 
GTGGAGACAG CAGTCTTGGG TCCTGAGAAC AATCTGATCA TCACAGGGTC CCGGGATGCA 
CTTATCCAGG TGTGGAGTTT GTCAGAACAG GGGACCCTGC TGAATGTCCT TGAGGGCGTG 
GGCGCCCCTG TGAGTCTGCT GGTCCGGGGT GGGACTCTGG TGGTGTCTGC ATCCCGGAAG 
TCTTCATCTT TCAAGGTCTG GGATCTCAAG TCCACTAAGA AGCTGCAGTC TCCGACTCCA 
TTTCTGGACC GCACCGGCCT CGCTGCAGTG TCCCACCATG GCAGCTTTGT CTACTTCCCT 
AAGGTTGGGG ACAAAAACAA AGTCACAATT TGGGACTTGG CAGAAGGTGA GGAGCAAGAT 
TGCCTGGACA CGTCCAATGA GGTCCGCTGT CTGGAGGTGG CCGAGCAGGC CAAGCTCCTC 
TTCACAGGCC TGGTTTCTGG GATCGTGCTT GTGTTCCCCC TGAATTCCAG GCAAGATGTA 
CTGTGCATCC CCCCACCTGA GGCACGCAAA GCTGTCAACT GCATGTCCCT GAGCAAGAGT 
GAGAATCGTC TGGCCATTGC CTATGACAAC ATTGTCCTGG TGCTTGACAT CAGCCCTGGG 
GACCCATGTC CGGCCATTGA AGGCCCCACC TACACCTTTT ACACCCAGCT GCCTGAGACC 
ATAGTGAGTG TGGCTGTCCT GGCTGACTAT CGTGTGGTCT ACGGCATGAG CGATGGCAGC 
CTCTTCCTGT ACGACTGTGC ATGCTCTAAA GTGTTCCCTC TGGAGGCCCA TGGGAGCAGG 
GTGAGCTGTG TAGAGGTCAG CCACAGTGAG CAGCTAGCAG TGAGCGGGGC TGAAGATGCA 
CTGCTATGCC TCTGGGACCT GCAGGCTTGC AGGGGGATGT TCGAGATGAG CTATGAGAAC 
TCCTGCTGCA GAGGGGTGCG GTGTGCCTGC TTCTCCAGGG ATGACAAGCA TGTATTTGCA 
GGCATGGAGG ACCGCTCTGT CACTGCCTGG AGCACGGTGG ATGGCACTCT TTTGGCTGTC 
CAGTTTGTTC ATGCTGTGAT CAACCGAATC ATCCCTACCT CCAATGGCTT CATGGCCCCC 
ACAAGCCACG GCTATCTCAT CCGTGAGCGC TTCCAGTGCC CGTCAGTCAG AGCCTCCCAG 
CAAGACCCTC TCAAGAACTT CAAGAAGGCA GTGTGGCTGG TAAAGACCAG GCAGAGGGAA 
GAGCTGGCTG CAGCAGAAGC CTCCCAGGAT GCAGAACCAG TGGCCGTAGA GGGAAAGGAA 
TCAAAGTCAA ATAAACGCTC ACAGGTCTGC CTCATACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099465.1
CDS	1278..5519
Translation	MCYHRSVIEWEIELGLLSSARGDQGATVFLRDVQDLNKHILDDCSLKMVDRLVDGCLDTNAQSLLSGLKGRILDAQPGALKSHHLSWSRDLVNPKNKAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPGIGKTSLMCKLAQQVPELLGHKTVVVLRLLGTSKLSLDARSLLRSLSFQVCLAYGLPLPPAQVLEAHSRVGHFFHTLLHTVSQRNFESLVLLLDSVDDLDSICHSPRVSWLPLKCPPRVHLILSTCSGQQVLHNLQQTLKDPSTYWEVKALSGSQGQEFIQLLLAAEKRMLSPGQRDVLWASLPECGHPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCIRLEETHGALLVAHVLGYIVSSRDLDRTWILWRYGLSEAELKDVLSLDDEVLQAVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLVRRPVDGCMLLAIAHRQLSQVIQVRYLSGPERAKRHGVLAEFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSSTVANLRKLTELPFHLLHAGRLEELKQEVLGNMSWISCRGISGGIEVLLDDFDMCAPHMNSPEVDLVREAIQLCGPAVELRGLEKSVLYTELLARLLFFAASHPAMIGQLCQQAQSWFRACPYPMLVPLAGFLQPPGGPLRATLTGCHKGITAIAWSLEEKLLVVGTQDGAMVVWDVEEQQVVHVLMGHTAEVKCVRVFAQGTLAISASKDHTLRLWSLLSGQEKVTILDGGSQNPTEPQSWDLHVDERNNVVYSTSGARINMWNLETSKLVFCITGDVSDPWVCVALLAAQGLLLALSKGGQVSLWSSAMGKLQEKHQLSSIKEETPTCAVSIQSRARLVAGFSSGSIALVSAGEDRLLEKLPEAVGFLVVSEDDSLLVAGFGRFVRIFLADSQGFHRFMASDLEHEDMVETAVLGPENNLIITGSRDALIQVWSLSEQGTLLNVLEGVGAPVSLLVRGGTLVVSASRKSSSFKVWDLKSTKKLQSPTPFLDRTGLAAVSHHGSFVYFPKVGDKNKVTIWDLAEGEEQDCLDTSNEVRCLEVAEQAKLLFTGLVSGIVLVFPLNSRQDVLCIPPPEARKAVNCMSLSKSENRLAIAYDNIVLVLDISPGDPCPAIEGPTYTFYTQLPETIVSVAVLADYRVVYGMSDGSLFLYDCACSKVFPLEAHGSRVSCVEVSHSEQLAVSGAEDALLCLWDLQACRGMFEMSYENSCCRGVRCACFSRDDKHVFAGMEDRSVTAWSTVDGTLLAVQFVHAVINRIIPTSNGFMAPTSHGYLIRERFQCPSVRASQQDPLKNFKKAVWLVKTRQREELAAAEASQDAEPVAVEGKESKSNKRSQVCLIL

Target ORF information:

RefSeq Version	XM_030243605.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243605.1

ORF Insert Sequence:

ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG GATTTGGACA GAACCTGGAT TTTGTGGAGG 
TACGGTCTCT CAGAAGCAGA GCTGAAGGAT GTGTTGTCTT TGGATGATGA AGTCTTGCAG 
GCTGTGTACC GGGACTGGAC GCCACCTAGC AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG 
TGGGTGCGGC TCCGCCGGGA CCTGGGACAC TGCCTAGTCA GGAGGCCCGT GGATGGCTGC 
ATGCTACTGG CCATTGCACA CAGACAGCTG TCCCAGGTAA TCCAAGTTCG ATATCTGTCT 
GGGCCTGAGA GAGCCAAGAG GCATGGCGTG CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC 
CAGGGGATCA AGAAACTTAT CACCCTGCCG CTTGTAGGGA AGCCCCTGAA CCTGGACCGC 
AAGGTGGCCC CACAGCCACT GTGGTTCTCA AGCACAGTGG CCAACCTGAG GAAGCTGACC 
GAACTGCCCT TCCATCTGCT GCATGCAGGC CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG 
GGGAACATGA GTTGGATCTC ATGTCGGGGC ATCTCTGGAG GCATTGAGGT CCTGCTGGAT 
GATTTTGACA TGTGTGCCCC TCACATGAAC TCTCCTGAGG TTGATCTGGT TAGAGAAGCC 
ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC CGGGGCCTGG AGAAGAGCGT CCTATACACC 
GAGCTCCTGG CCAGACTCCT TTTCTTTGCT GCATCACATC CAGCAATGAT CGGACAACTG 
TGCCAACAAG CCCAGAGCTG GTTCCGTGCC TGCCCCTACC CAATGCTGGT GCCCCTGGCA 
GGATTCCTGC AGCCACCTGG TGGACCTCTT CGAGCCACTC TTACTGGGTG TCACAAAGGC 
ATCACCGCCA TAGCATGGAG CCTGGAGGAG AAACTCCTGG TGGTGGGCAC CCAGGATGGT 
GCCATGGTCG TGTGGGACGT GGAGGAACAA CAGGTGGTAC ACGTTCTAAT GGGACACACA 
GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC CAAGGGACTC TTGCCATCTC TGCATCAAAG 
GACCATACCC TGCGTCTGTG GAGTCTGCTT TCCGGCCAGG AGAAAGTCAC CATTTTGGAT 
GGAGGGTCAC AAAATCCCAC AGAGCCCCAG AGTTGGGACC TTCATGTGGA TGAGAGAAAC 
AATGTTGTAT ACTCGACATC TGGTGCAAGG ATCAACATGT GGAATCTGGA AACTTCGAAG 
CTGGTTTTCT GTATCACAGG AGATGTGTCT GATCCCTGGG TCTGTGTGGC TTTGTTGGCT 
GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC 
ATGGGAAAAC TGCAGGAGAA ACACCAGTTA TCCAGCATCA AAGAAGAGAC ACCCACCTGT 
GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG GTCGCTGGCT TCAGCAGTGG CTCCATTGCC 
CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG 
GTGGTTTCTG AAGACGATTC CCTTCTTGTG GCAGGCTTTG GAAGATTTGT GCGGATTTTC 
TTGGCTGACT CGCAAGGCTT CCACCGGTTC ATGGCTTCGG ATCTGGAACA CGAGGACATG 
GTGGAGACAG CAGTCTTGGG TCCTGAGAAC AATCTGATCA TCACAGGGTC CCGGGATGCA 
CTTATCCAGG TGTGGAGTTT GTCAGAACAG GGGACCCTGC TGAATGTCCT TGAGGGCGTG 
GGCGCCCCTG TGAGTCTGCT GGTCCGGGGT GGGACTCTGG TGGTGTCTGC ATCCCGGAAG 
TCTTCATCTT TCAAGGTCTG GGATCTCAAG TCCACTAAGA AGCTGCAGTC TCCGACTCCA 
TTTCTGGACC GCACCGGCCT CGCTGCAGTG TCCCACCATG GCAGCTTTGT CTACTTCCCT 
AAGGTTGGGG ACAAAAACAA AGTCACAATT TGGGACTTGG CAGAAGGTGA GGAGCAAGAT 
TGCCTGGACA CGTCCAATGA GGTCCGCTGT CTGGAGGTGG CCGAGCAGGC CAAGCTCCTC 
TTCACAGGCC TGGTTTCTGG GATCGTGCTT GTGTTCCCCC TGAATTCCAG GCAAGATGTA 
CTGTGCATCC CCCCACCTGA GGCACGCAAA GCTGTCAACT GCATGTCCCT GAGCAAGAGT 
GAGAATCGTC TGGCCATTGC CTATGACAAC ATTGTCCTGG TGCTTGACAT CAGCCCTGGG 
GACCCATGTC CGGCCATTGA AGGCCCCACC TACACCTTTT ACACCCAGCT GCCTGAGACC 
ATAGTGAGTG TGGCTGTCCT GGCTGACTAT CGTGTGGTCT ACGGCATGAG CGATGGCAGC 
CTCTTCCTGT ACGACTGTGC ATGCTCTAAA GTGTTCCCTC TGGAGGCCCA TGGGAGCAGG 
GTGAGCTGTG TAGAGGTCAG CCACAGTGAG CAGCTAGCAG TGAGCGGGGC TGAAGATGCA 
CTGCTATGCC TCTGGGACCT GCAGGCTTGC AGGGGGATGT TCGAGATGAG CTATGAGAAC 
TCCTGCTGCA GAGGGGTGCG GTGTGCCTGC TTCTCCAGGG ATGACAAGCA TGTATTTGCA 
GGCATGGAGG ACCGCTCTGT CACTGCCTGG AGCACGGTGG ATGGCACTCT TTTGGCTGTC 
CAGTTTGTTC ATGCTGTGAT CAACCGAATC ATCCCTACCT CCAATGGCTT CATGGCCCCC 
ACAAGCCACG GCTATCTCAT CCGTGAGCGC TTCCAGTGCC CGTCAGTCAG AGCCTCCCAG 
CAAGACCCTC TCAAGAACTT CAAGAAGGCA GTGTGGCTGG TAAAGACCAG GCAGAGGGAA 
GAGCTGGCTG CAGCAGAAGC CTCCCAGGAT GCAGAACCAG TGGCCGTAGA GGGAAAGGAA 
TCAAAGTCAA ATAAACGCTC ACAGGTCTGC CTCATACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nwd1 ( XM_030243607.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099467.1 Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu89869
Clone ID Related Accession (Same CDS sequence)	XM_030243607.1 , XM_030243606.1
Accession Version	XM_030243607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATC GGCTGGTAGA TGGCTGCCTG GATACCAATG CCCAGAGTCT TCTCAGTGGT 
CTTAAAGGGC GCATACTAGA TGCTCAGCCT GGAGCCCTAA AGTCCCACCA CCTGTCCTGG 
AGCCGAGACC TGGTCAACCC CAAGAACAAG GCTCACGCTC GTTACCTAAA GCAGTTGAGC 
GAGCAGTTCG TGGCCAGGAC CAACCATCAG GTCCTGGAGC AGCTTCGAGA GCTGGAGTTG 
GCCAGACAGG AGCTGGGTTG GCTCTACCAA GAGATCCGCC ACCACCTCTG GCAGAGCACA 
GAGTCTACCA AGGTCTTCTG CGGCCACCAG GAGCTCCTGG CCCAGCTCAG GCAGCAGCTC 
AGACAGGATG AGAGCCGGAC ACATACTCCC TTGGTTCTCT TTGGACCACC AGGCATCGGG 
AAGACCTCTC TCATGTGCAA GCTAGCCCAG CAGGTACCAG AGCTACTTGG CCACAAGACA 
GTGGTTGTCC TGAGGCTGCT AGGGACATCA AAGCTGAGCC TGGATGCCCG CAGCCTCCTC 
AGGAGCCTGT CCTTCCAAGT GTGCTTGGCA TATGGGCTGC CACTGCCCCC AGCCCAGGTC 
CTGGAGGCCC ACAGCAGGGT GGGCCATTTC TTCCATACTC TCCTCCACAC CGTGTCACAG 
AGGAACTTTG AGTCCCTGGT GTTGCTCCTG GACTCCGTGG ATGACCTGGA CTCCATATGC 
CATTCTCCGA GGGTCTCTTG GCTGCCCCTC AAGTGTCCTC CAAGGGTGCA CCTCATCCTC 
TCTACATGCT CAGGCCAGCA GGTTTTACAT AACCTGCAAC AGACCCTCAA GGACCCCAGC 
ACCTACTGGG AGGTGAAGGC CCTGTCTGGA AGTCAAGGCC AGGAGTTCAT ACAGCTGCTG 
CTGGCTGCAG AGAAGAGGAT GCTGAGCCCG GGTCAGAGAG ATGTCCTCTG GGCCAGCCTC 
CCAGAGTGTG GCCACCCAGG GAGGCTAAGG CTGGCCTTTG AGGAGGCCCG GAAGTGGGCC 
TCCTTCACTG TGCCAGTGCC CTTGGCCACT ACAGCAGAGG AAGCCACACA CCAGCTCTGT 
ATACGCCTGG AGGAGACACA TGGGGCACTG CTGGTGGCTC ATGTACTGGG CTATATTGTG 
TCTTCCAGGT ACGGTCTCTC AGAAGCAGAG CTGAAGGATG TGTTGTCTTT GGATGATGAA 
GTCTTGCAGG CTGTGTACCG GGACTGGACG CCACCTAGCA AAGAGCTGCT GCGCTTCCCG 
CCCCTGCTGT GGGTGCGGCT CCGCCGGGAC CTGGGACACT GCCTAGTCAG GAGGCCCGTG 
GATGGCTGCA TGCTACTGGC CATTGCACAC AGACAGCTGT CCCAGGTAAT CCAAGTTCGA 
TATCTGTCTG GGCCTGAGAG AGCCAAGAGG CATGGCGTGC TAGCTGAATT CTTCTCTGGA 
GCCTGGAGCC AGGGGATCAA GAAACTTATC ACCCTGCCGC TTGTAGGGAA GCCCCTGAAC 
CTGGACCGCA AGGTGGCCCC ACAGCCACTG TGGTTCTCAA GCACAGTGGC CAACCTGAGG 
AAGCTGACCG AACTGCCCTT CCATCTGCTG CATGCAGGCC GCTTGGAGGA GTTGAAGCAG 
GAGGTTCTGG GGAACATGAG TTGGATCTCA TGTCGGGGCA TCTCTGGAGG CATTGAGGTC 
CTGCTGGATG ATTTTGACAT GTGTGCCCCT CACATGAACT CTCCTGAGGT TGATCTGGTT 
AGAGAAGCCA TCCAGCTGTG TGGCCCTGCT GTGGAGCTCC GGGGCCTGGA GAAGAGCGTC 
CTATACACCG AGCTCCTGGC CAGACTCCTT TTCTTTGCTG CATCACATCC AGCAATGATC 
GGACAACTGT GCCAACAAGC CCAGAGCTGG TTCCGTGCCT GCCCCTACCC AATGCTGGTG 
CCCCTGGCAG GATTCCTGCA GCCACCTGGT GGACCTCTTC GAGCCACTCT TACTGGGTGT 
CACAAAGGCA TCACCGCCAT AGCATGGAGC CTGGAGGAGA AACTCCTGGT GGTGGGCACC 
CAGGATGGTG CCATGGTCGT GTGGGACGTG GAGGAACAAC AGGTGGTACA CGTTCTAATG 
GGACACACAG CTGAAGTGAA GTGTGTGCGA GTGTTTGCCC AAGGGACTCT TGCCATCTCT 
GCATCAAAGG ACCATACCCT GCGTCTGTGG AGTCTGCTTT CCGGCCAGGA GAAAGTCACC 
ATTTTGGATG GAGGGTCACA AAATCCCACA GAGCCCCAGA GTTGGGACCT TCATGTGGAT 
GAGAGAAACA ATGTTGTATA CTCGACATCT GGTGCAAGGA TCAACATGTG GAATCTGGAA 
ACTTCGAAGC TGGTTTTCTG TATCACAGGA GATGTGTCTG ATCCCTGGGT CTGTGTGGCT 
TTGTTGGCTG CCCAGGGTTT GCTGCTTGCC CTGTCCAAGG GTGGCCAGGT CAGTCTGTGG 
AGCTCAGCCA TGGGAAAACT GCAGGAGAAA CACCAGTTAT CCAGCATCAA AGAAGAGACA 
CCCACCTGTG CCGTCTCCAT CCAGTCCCGG GCAAGGCTGG TCGCTGGCTT CAGCAGTGGC 
TCCATTGCCC TGGTCTCCGC TGGAGAAGAC AGGCTGCTGG AGAAGCTCCC AGAAGCTGTG 
GGGTTCCTGG TGGTTTCTGA AGACGATTCC CTTCTTGTGG CAGGCTTTGG AAGATTTGTG 
CGGATTTTCT TGGCTGACTC GCAAGGCTTC CACCGGTTCA TGGCTTCGGA TCTGGAACAC 
GAGGACATGG TGGAGACAGC AGTCTTGGGT CCTGAGAACA ATCTGATCAT CACAGGGTCC 
CGGGATGCAC TTATCCAGGT GTGGAGTTTG TCAGAACAGG GGACCCTGCT GAATGTCCTT 
GAGGGCGTGG GCGCCCCTGT GAGTCTGCTG GTCCGGGGTG GGACTCTGGT GGTGTCTGCA 
TCCCGGAAGT CTTCATCTTT CAAGGTCTGG GATCTCAAGT CCACTAAGAA GCTGCAGTCT 
CCGACTCCAT TTCTGGACCG CACCGGCCTC GCTGCAGTGT CCCACCATGG CAGCTTTGTC 
TACTTCCCTA AGGTTGGGGA CAAAAACAAA GTCACAATTT GGGACTTGGC AGAAGGTGAG 
GAGCAAGATT GCCTGGACAC GTCCAATGAG GTCCGCTGTC TGGAGGTGGC CGAGCAGGCC 
AAGCTCCTCT TCACAGGCCT GGTTTCTGGG ATCGTGCTTG TGTTCCCCCT GAATTCCAGG 
CAAGATGTAC TGTGCATCCC CCCACCTGAG GCACGCAAAG CTGTCAACTG CATGTCCCTG 
AGCAAGAGTG AGAATCGTCT GGCCATTGCC TATGACAACA TTGTCCTGGT GCTTGACATC 
AGCCCTGGGG ACCCATGTCC GGCCATTGAA GGCCCCACCT ACACCTTTTA CACCCAGCTG 
CCTGAGACCA TAGTGAGTGT GGCTGTCCTG GCTGACTATC GTGTGGTCTA CGGCATGAGC 
GATGGCAGCC TCTTCCTGTA CGACTGTGCA TGCTCTAAAG TGTTCCCTCT GGAGGCCCAT 
GGGAGCAGGG TGAGCTGTGT AGAGGTCAGC CACAGTGAGC AGCTAGCAGT GAGCGGGGCT 
GAAGATGCAC TGCTATGCCT CTGGGACCTG CAGGCTTGCA GGGGGATGTT CGAGATGAGC 
TATGAGAACT CCTGCTGCAG AGGGGTGCGG TGTGCCTGCT TCTCCAGGGA TGACAAGCAT 
GTATTTGCAG GCATGGAGGA CCGCTCTGTC ACTGCCTGGA GCACGGTGGA TGGCACTCTT 
TTGGCTGTCC AGTTTGTTCA TGCTGTGATC AACCGAATCA TCCCTACCTC CAATGGCTTC 
ATGGCCCCCA CAAGCCACGG CTATCTCATC CGTGAGCGCT TCCAGTGCCC GTCAGTCAGA 
GCCTCCCAGC AAGACCCTCT CAAGAACTTC AAGAAGGCAG TGTGGCTGGT AAAGACCAGG 
CAGAGGGAAG AGCTGGCTGC AGCAGAAGCC TCCCAGGATG CAGAACCAGT GGCCGTAGAG 
GGAAAGGAAT CAAAGTCAAA TAAACGCTCA CAGGTCTGCC TCATACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099467.1
CDS	1121..5191
Translation	MVDRLVDGCLDTNAQSLLSGLKGRILDAQPGALKSHHLSWSRDLVNPKNKAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPGIGKTSLMCKLAQQVPELLGHKTVVVLRLLGTSKLSLDARSLLRSLSFQVCLAYGLPLPPAQVLEAHSRVGHFFHTLLHTVSQRNFESLVLLLDSVDDLDSICHSPRVSWLPLKCPPRVHLILSTCSGQQVLHNLQQTLKDPSTYWEVKALSGSQGQEFIQLLLAAEKRMLSPGQRDVLWASLPECGHPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCIRLEETHGALLVAHVLGYIVSSRYGLSEAELKDVLSLDDEVLQAVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLVRRPVDGCMLLAIAHRQLSQVIQVRYLSGPERAKRHGVLAEFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSSTVANLRKLTELPFHLLHAGRLEELKQEVLGNMSWISCRGISGGIEVLLDDFDMCAPHMNSPEVDLVREAIQLCGPAVELRGLEKSVLYTELLARLLFFAASHPAMIGQLCQQAQSWFRACPYPMLVPLAGFLQPPGGPLRATLTGCHKGITAIAWSLEEKLLVVGTQDGAMVVWDVEEQQVVHVLMGHTAEVKCVRVFAQGTLAISASKDHTLRLWSLLSGQEKVTILDGGSQNPTEPQSWDLHVDERNNVVYSTSGARINMWNLETSKLVFCITGDVSDPWVCVALLAAQGLLLALSKGGQVSLWSSAMGKLQEKHQLSSIKEETPTCAVSIQSRARLVAGFSSGSIALVSAGEDRLLEKLPEAVGFLVVSEDDSLLVAGFGRFVRIFLADSQGFHRFMASDLEHEDMVETAVLGPENNLIITGSRDALIQVWSLSEQGTLLNVLEGVGAPVSLLVRGGTLVVSASRKSSSFKVWDLKSTKKLQSPTPFLDRTGLAAVSHHGSFVYFPKVGDKNKVTIWDLAEGEEQDCLDTSNEVRCLEVAEQAKLLFTGLVSGIVLVFPLNSRQDVLCIPPPEARKAVNCMSLSKSENRLAIAYDNIVLVLDISPGDPCPAIEGPTYTFYTQLPETIVSVAVLADYRVVYGMSDGSLFLYDCACSKVFPLEAHGSRVSCVEVSHSEQLAVSGAEDALLCLWDLQACRGMFEMSYENSCCRGVRCACFSRDDKHVFAGMEDRSVTAWSTVDGTLLAVQFVHAVINRIIPTSNGFMAPTSHGYLIRERFQCPSVRASQQDPLKNFKKAVWLVKTRQREELAAAEASQDAEPVAVEGKESKSNKRSQVCLIL

Target ORF information:

RefSeq Version	XM_030243607.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243607.1

ORF Insert Sequence:

ATGGTGGATC GGCTGGTAGA TGGCTGCCTG GATACCAATG CCCAGAGTCT TCTCAGTGGT 
CTTAAAGGGC GCATACTAGA TGCTCAGCCT GGAGCCCTAA AGTCCCACCA CCTGTCCTGG 
AGCCGAGACC TGGTCAACCC CAAGAACAAG GCTCACGCTC GTTACCTAAA GCAGTTGAGC 
GAGCAGTTCG TGGCCAGGAC CAACCATCAG GTCCTGGAGC AGCTTCGAGA GCTGGAGTTG 
GCCAGACAGG AGCTGGGTTG GCTCTACCAA GAGATCCGCC ACCACCTCTG GCAGAGCACA 
GAGTCTACCA AGGTCTTCTG CGGCCACCAG GAGCTCCTGG CCCAGCTCAG GCAGCAGCTC 
AGACAGGATG AGAGCCGGAC ACATACTCCC TTGGTTCTCT TTGGACCACC AGGCATCGGG 
AAGACCTCTC TCATGTGCAA GCTAGCCCAG CAGGTACCAG AGCTACTTGG CCACAAGACA 
GTGGTTGTCC TGAGGCTGCT AGGGACATCA AAGCTGAGCC TGGATGCCCG CAGCCTCCTC 
AGGAGCCTGT CCTTCCAAGT GTGCTTGGCA TATGGGCTGC CACTGCCCCC AGCCCAGGTC 
CTGGAGGCCC ACAGCAGGGT GGGCCATTTC TTCCATACTC TCCTCCACAC CGTGTCACAG 
AGGAACTTTG AGTCCCTGGT GTTGCTCCTG GACTCCGTGG ATGACCTGGA CTCCATATGC 
CATTCTCCGA GGGTCTCTTG GCTGCCCCTC AAGTGTCCTC CAAGGGTGCA CCTCATCCTC 
TCTACATGCT CAGGCCAGCA GGTTTTACAT AACCTGCAAC AGACCCTCAA GGACCCCAGC 
ACCTACTGGG AGGTGAAGGC CCTGTCTGGA AGTCAAGGCC AGGAGTTCAT ACAGCTGCTG 
CTGGCTGCAG AGAAGAGGAT GCTGAGCCCG GGTCAGAGAG ATGTCCTCTG GGCCAGCCTC 
CCAGAGTGTG GCCACCCAGG GAGGCTAAGG CTGGCCTTTG AGGAGGCCCG GAAGTGGGCC 
TCCTTCACTG TGCCAGTGCC CTTGGCCACT ACAGCAGAGG AAGCCACACA CCAGCTCTGT 
ATACGCCTGG AGGAGACACA TGGGGCACTG CTGGTGGCTC ATGTACTGGG CTATATTGTG 
TCTTCCAGGT ACGGTCTCTC AGAAGCAGAG CTGAAGGATG TGTTGTCTTT GGATGATGAA 
GTCTTGCAGG CTGTGTACCG GGACTGGACG CCACCTAGCA AAGAGCTGCT GCGCTTCCCG 
CCCCTGCTGT GGGTGCGGCT CCGCCGGGAC CTGGGACACT GCCTAGTCAG GAGGCCCGTG 
GATGGCTGCA TGCTACTGGC CATTGCACAC AGACAGCTGT CCCAGGTAAT CCAAGTTCGA 
TATCTGTCTG GGCCTGAGAG AGCCAAGAGG CATGGCGTGC TAGCTGAATT CTTCTCTGGA 
GCCTGGAGCC AGGGGATCAA GAAACTTATC ACCCTGCCGC TTGTAGGGAA GCCCCTGAAC 
CTGGACCGCA AGGTGGCCCC ACAGCCACTG TGGTTCTCAA GCACAGTGGC CAACCTGAGG 
AAGCTGACCG AACTGCCCTT CCATCTGCTG CATGCAGGCC GCTTGGAGGA GTTGAAGCAG 
GAGGTTCTGG GGAACATGAG TTGGATCTCA TGTCGGGGCA TCTCTGGAGG CATTGAGGTC 
CTGCTGGATG ATTTTGACAT GTGTGCCCCT CACATGAACT CTCCTGAGGT TGATCTGGTT 
AGAGAAGCCA TCCAGCTGTG TGGCCCTGCT GTGGAGCTCC GGGGCCTGGA GAAGAGCGTC 
CTATACACCG AGCTCCTGGC CAGACTCCTT TTCTTTGCTG CATCACATCC AGCAATGATC 
GGACAACTGT GCCAACAAGC CCAGAGCTGG TTCCGTGCCT GCCCCTACCC AATGCTGGTG 
CCCCTGGCAG GATTCCTGCA GCCACCTGGT GGACCTCTTC GAGCCACTCT TACTGGGTGT 
CACAAAGGCA TCACCGCCAT AGCATGGAGC CTGGAGGAGA AACTCCTGGT GGTGGGCACC 
CAGGATGGTG CCATGGTCGT GTGGGACGTG GAGGAACAAC AGGTGGTACA CGTTCTAATG 
GGACACACAG CTGAAGTGAA GTGTGTGCGA GTGTTTGCCC AAGGGACTCT TGCCATCTCT 
GCATCAAAGG ACCATACCCT GCGTCTGTGG AGTCTGCTTT CCGGCCAGGA GAAAGTCACC 
ATTTTGGATG GAGGGTCACA AAATCCCACA GAGCCCCAGA GTTGGGACCT TCATGTGGAT 
GAGAGAAACA ATGTTGTATA CTCGACATCT GGTGCAAGGA TCAACATGTG GAATCTGGAA 
ACTTCGAAGC TGGTTTTCTG TATCACAGGA GATGTGTCTG ATCCCTGGGT CTGTGTGGCT 
TTGTTGGCTG CCCAGGGTTT GCTGCTTGCC CTGTCCAAGG GTGGCCAGGT CAGTCTGTGG 
AGCTCAGCCA TGGGAAAACT GCAGGAGAAA CACCAGTTAT CCAGCATCAA AGAAGAGACA 
CCCACCTGTG CCGTCTCCAT CCAGTCCCGG GCAAGGCTGG TCGCTGGCTT CAGCAGTGGC 
TCCATTGCCC TGGTCTCCGC TGGAGAAGAC AGGCTGCTGG AGAAGCTCCC AGAAGCTGTG 
GGGTTCCTGG TGGTTTCTGA AGACGATTCC CTTCTTGTGG CAGGCTTTGG AAGATTTGTG 
CGGATTTTCT TGGCTGACTC GCAAGGCTTC CACCGGTTCA TGGCTTCGGA TCTGGAACAC 
GAGGACATGG TGGAGACAGC AGTCTTGGGT CCTGAGAACA ATCTGATCAT CACAGGGTCC 
CGGGATGCAC TTATCCAGGT GTGGAGTTTG TCAGAACAGG GGACCCTGCT GAATGTCCTT 
GAGGGCGTGG GCGCCCCTGT GAGTCTGCTG GTCCGGGGTG GGACTCTGGT GGTGTCTGCA 
TCCCGGAAGT CTTCATCTTT CAAGGTCTGG GATCTCAAGT CCACTAAGAA GCTGCAGTCT 
CCGACTCCAT TTCTGGACCG CACCGGCCTC GCTGCAGTGT CCCACCATGG CAGCTTTGTC 
TACTTCCCTA AGGTTGGGGA CAAAAACAAA GTCACAATTT GGGACTTGGC AGAAGGTGAG 
GAGCAAGATT GCCTGGACAC GTCCAATGAG GTCCGCTGTC TGGAGGTGGC CGAGCAGGCC 
AAGCTCCTCT TCACAGGCCT GGTTTCTGGG ATCGTGCTTG TGTTCCCCCT GAATTCCAGG 
CAAGATGTAC TGTGCATCCC CCCACCTGAG GCACGCAAAG CTGTCAACTG CATGTCCCTG 
AGCAAGAGTG AGAATCGTCT GGCCATTGCC TATGACAACA TTGTCCTGGT GCTTGACATC 
AGCCCTGGGG ACCCATGTCC GGCCATTGAA GGCCCCACCT ACACCTTTTA CACCCAGCTG 
CCTGAGACCA TAGTGAGTGT GGCTGTCCTG GCTGACTATC GTGTGGTCTA CGGCATGAGC 
GATGGCAGCC TCTTCCTGTA CGACTGTGCA TGCTCTAAAG TGTTCCCTCT GGAGGCCCAT 
GGGAGCAGGG TGAGCTGTGT AGAGGTCAGC CACAGTGAGC AGCTAGCAGT GAGCGGGGCT 
GAAGATGCAC TGCTATGCCT CTGGGACCTG CAGGCTTGCA GGGGGATGTT CGAGATGAGC 
TATGAGAACT CCTGCTGCAG AGGGGTGCGG TGTGCCTGCT TCTCCAGGGA TGACAAGCAT 
GTATTTGCAG GCATGGAGGA CCGCTCTGTC ACTGCCTGGA GCACGGTGGA TGGCACTCTT 
TTGGCTGTCC AGTTTGTTCA TGCTGTGATC AACCGAATCA TCCCTACCTC CAATGGCTTC 
ATGGCCCCCA CAAGCCACGG CTATCTCATC CGTGAGCGCT TCCAGTGCCC GTCAGTCAGA 
GCCTCCCAGC AAGACCCTCT CAAGAACTTC AAGAAGGCAG TGTGGCTGGT AAAGACCAGG 
CAGAGGGAAG AGCTGGCTGC AGCAGAAGCC TCCCAGGATG CAGAACCAGT GGCCGTAGAG 
GGAAAGGAAT CAAAGTCAAA TAAACGCTCA CAGGTCTGCC TCATACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nwd1 ( XM_030243606.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099466.1 Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X7, mRNA.

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CloneID	OMu89869
Clone ID Related Accession (Same CDS sequence)	XM_030243607.1 , XM_030243606.1
Accession Version	XM_030243606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATC GGCTGGTAGA TGGCTGCCTG GATACCAATG CCCAGAGTCT TCTCAGTGGT 
CTTAAAGGGC GCATACTAGA TGCTCAGCCT GGAGCCCTAA AGTCCCACCA CCTGTCCTGG 
AGCCGAGACC TGGTCAACCC CAAGAACAAG GCTCACGCTC GTTACCTAAA GCAGTTGAGC 
GAGCAGTTCG TGGCCAGGAC CAACCATCAG GTCCTGGAGC AGCTTCGAGA GCTGGAGTTG 
GCCAGACAGG AGCTGGGTTG GCTCTACCAA GAGATCCGCC ACCACCTCTG GCAGAGCACA 
GAGTCTACCA AGGTCTTCTG CGGCCACCAG GAGCTCCTGG CCCAGCTCAG GCAGCAGCTC 
AGACAGGATG AGAGCCGGAC ACATACTCCC TTGGTTCTCT TTGGACCACC AGGCATCGGG 
AAGACCTCTC TCATGTGCAA GCTAGCCCAG CAGGTACCAG AGCTACTTGG CCACAAGACA 
GTGGTTGTCC TGAGGCTGCT AGGGACATCA AAGCTGAGCC TGGATGCCCG CAGCCTCCTC 
AGGAGCCTGT CCTTCCAAGT GTGCTTGGCA TATGGGCTGC CACTGCCCCC AGCCCAGGTC 
CTGGAGGCCC ACAGCAGGGT GGGCCATTTC TTCCATACTC TCCTCCACAC CGTGTCACAG 
AGGAACTTTG AGTCCCTGGT GTTGCTCCTG GACTCCGTGG ATGACCTGGA CTCCATATGC 
CATTCTCCGA GGGTCTCTTG GCTGCCCCTC AAGTGTCCTC CAAGGGTGCA CCTCATCCTC 
TCTACATGCT CAGGCCAGCA GGTTTTACAT AACCTGCAAC AGACCCTCAA GGACCCCAGC 
ACCTACTGGG AGGTGAAGGC CCTGTCTGGA AGTCAAGGCC AGGAGTTCAT ACAGCTGCTG 
CTGGCTGCAG AGAAGAGGAT GCTGAGCCCG GGTCAGAGAG ATGTCCTCTG GGCCAGCCTC 
CCAGAGTGTG GCCACCCAGG GAGGCTAAGG CTGGCCTTTG AGGAGGCCCG GAAGTGGGCC 
TCCTTCACTG TGCCAGTGCC CTTGGCCACT ACAGCAGAGG AAGCCACACA CCAGCTCTGT 
ATACGCCTGG AGGAGACACA TGGGGCACTG CTGGTGGCTC ATGTACTGGG CTATATTGTG 
TCTTCCAGGT ACGGTCTCTC AGAAGCAGAG CTGAAGGATG TGTTGTCTTT GGATGATGAA 
GTCTTGCAGG CTGTGTACCG GGACTGGACG CCACCTAGCA AAGAGCTGCT GCGCTTCCCG 
CCCCTGCTGT GGGTGCGGCT CCGCCGGGAC CTGGGACACT GCCTAGTCAG GAGGCCCGTG 
GATGGCTGCA TGCTACTGGC CATTGCACAC AGACAGCTGT CCCAGGTAAT CCAAGTTCGA 
TATCTGTCTG GGCCTGAGAG AGCCAAGAGG CATGGCGTGC TAGCTGAATT CTTCTCTGGA 
GCCTGGAGCC AGGGGATCAA GAAACTTATC ACCCTGCCGC TTGTAGGGAA GCCCCTGAAC 
CTGGACCGCA AGGTGGCCCC ACAGCCACTG TGGTTCTCAA GCACAGTGGC CAACCTGAGG 
AAGCTGACCG AACTGCCCTT CCATCTGCTG CATGCAGGCC GCTTGGAGGA GTTGAAGCAG 
GAGGTTCTGG GGAACATGAG TTGGATCTCA TGTCGGGGCA TCTCTGGAGG CATTGAGGTC 
CTGCTGGATG ATTTTGACAT GTGTGCCCCT CACATGAACT CTCCTGAGGT TGATCTGGTT 
AGAGAAGCCA TCCAGCTGTG TGGCCCTGCT GTGGAGCTCC GGGGCCTGGA GAAGAGCGTC 
CTATACACCG AGCTCCTGGC CAGACTCCTT TTCTTTGCTG CATCACATCC AGCAATGATC 
GGACAACTGT GCCAACAAGC CCAGAGCTGG TTCCGTGCCT GCCCCTACCC AATGCTGGTG 
CCCCTGGCAG GATTCCTGCA GCCACCTGGT GGACCTCTTC GAGCCACTCT TACTGGGTGT 
CACAAAGGCA TCACCGCCAT AGCATGGAGC CTGGAGGAGA AACTCCTGGT GGTGGGCACC 
CAGGATGGTG CCATGGTCGT GTGGGACGTG GAGGAACAAC AGGTGGTACA CGTTCTAATG 
GGACACACAG CTGAAGTGAA GTGTGTGCGA GTGTTTGCCC AAGGGACTCT TGCCATCTCT 
GCATCAAAGG ACCATACCCT GCGTCTGTGG AGTCTGCTTT CCGGCCAGGA GAAAGTCACC 
ATTTTGGATG GAGGGTCACA AAATCCCACA GAGCCCCAGA GTTGGGACCT TCATGTGGAT 
GAGAGAAACA ATGTTGTATA CTCGACATCT GGTGCAAGGA TCAACATGTG GAATCTGGAA 
ACTTCGAAGC TGGTTTTCTG TATCACAGGA GATGTGTCTG ATCCCTGGGT CTGTGTGGCT 
TTGTTGGCTG CCCAGGGTTT GCTGCTTGCC CTGTCCAAGG GTGGCCAGGT CAGTCTGTGG 
AGCTCAGCCA TGGGAAAACT GCAGGAGAAA CACCAGTTAT CCAGCATCAA AGAAGAGACA 
CCCACCTGTG CCGTCTCCAT CCAGTCCCGG GCAAGGCTGG TCGCTGGCTT CAGCAGTGGC 
TCCATTGCCC TGGTCTCCGC TGGAGAAGAC AGGCTGCTGG AGAAGCTCCC AGAAGCTGTG 
GGGTTCCTGG TGGTTTCTGA AGACGATTCC CTTCTTGTGG CAGGCTTTGG AAGATTTGTG 
CGGATTTTCT TGGCTGACTC GCAAGGCTTC CACCGGTTCA TGGCTTCGGA TCTGGAACAC 
GAGGACATGG TGGAGACAGC AGTCTTGGGT CCTGAGAACA ATCTGATCAT CACAGGGTCC 
CGGGATGCAC TTATCCAGGT GTGGAGTTTG TCAGAACAGG GGACCCTGCT GAATGTCCTT 
GAGGGCGTGG GCGCCCCTGT GAGTCTGCTG GTCCGGGGTG GGACTCTGGT GGTGTCTGCA 
TCCCGGAAGT CTTCATCTTT CAAGGTCTGG GATCTCAAGT CCACTAAGAA GCTGCAGTCT 
CCGACTCCAT TTCTGGACCG CACCGGCCTC GCTGCAGTGT CCCACCATGG CAGCTTTGTC 
TACTTCCCTA AGGTTGGGGA CAAAAACAAA GTCACAATTT GGGACTTGGC AGAAGGTGAG 
GAGCAAGATT GCCTGGACAC GTCCAATGAG GTCCGCTGTC TGGAGGTGGC CGAGCAGGCC 
AAGCTCCTCT TCACAGGCCT GGTTTCTGGG ATCGTGCTTG TGTTCCCCCT GAATTCCAGG 
CAAGATGTAC TGTGCATCCC CCCACCTGAG GCACGCAAAG CTGTCAACTG CATGTCCCTG 
AGCAAGAGTG AGAATCGTCT GGCCATTGCC TATGACAACA TTGTCCTGGT GCTTGACATC 
AGCCCTGGGG ACCCATGTCC GGCCATTGAA GGCCCCACCT ACACCTTTTA CACCCAGCTG 
CCTGAGACCA TAGTGAGTGT GGCTGTCCTG GCTGACTATC GTGTGGTCTA CGGCATGAGC 
GATGGCAGCC TCTTCCTGTA CGACTGTGCA TGCTCTAAAG TGTTCCCTCT GGAGGCCCAT 
GGGAGCAGGG TGAGCTGTGT AGAGGTCAGC CACAGTGAGC AGCTAGCAGT GAGCGGGGCT 
GAAGATGCAC TGCTATGCCT CTGGGACCTG CAGGCTTGCA GGGGGATGTT CGAGATGAGC 
TATGAGAACT CCTGCTGCAG AGGGGTGCGG TGTGCCTGCT TCTCCAGGGA TGACAAGCAT 
GTATTTGCAG GCATGGAGGA CCGCTCTGTC ACTGCCTGGA GCACGGTGGA TGGCACTCTT 
TTGGCTGTCC AGTTTGTTCA TGCTGTGATC AACCGAATCA TCCCTACCTC CAATGGCTTC 
ATGGCCCCCA CAAGCCACGG CTATCTCATC CGTGAGCGCT TCCAGTGCCC GTCAGTCAGA 
GCCTCCCAGC AAGACCCTCT CAAGAACTTC AAGAAGGCAG TGTGGCTGGT AAAGACCAGG 
CAGAGGGAAG AGCTGGCTGC AGCAGAAGCC TCCCAGGATG CAGAACCAGT GGCCGTAGAG 
GGAAAGGAAT CAAAGTCAAA TAAACGCTCA CAGGTCTGCC TCATACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099466.1
CDS	1107..5177
Translation	MVDRLVDGCLDTNAQSLLSGLKGRILDAQPGALKSHHLSWSRDLVNPKNKAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPGIGKTSLMCKLAQQVPELLGHKTVVVLRLLGTSKLSLDARSLLRSLSFQVCLAYGLPLPPAQVLEAHSRVGHFFHTLLHTVSQRNFESLVLLLDSVDDLDSICHSPRVSWLPLKCPPRVHLILSTCSGQQVLHNLQQTLKDPSTYWEVKALSGSQGQEFIQLLLAAEKRMLSPGQRDVLWASLPECGHPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCIRLEETHGALLVAHVLGYIVSSRYGLSEAELKDVLSLDDEVLQAVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLVRRPVDGCMLLAIAHRQLSQVIQVRYLSGPERAKRHGVLAEFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSSTVANLRKLTELPFHLLHAGRLEELKQEVLGNMSWISCRGISGGIEVLLDDFDMCAPHMNSPEVDLVREAIQLCGPAVELRGLEKSVLYTELLARLLFFAASHPAMIGQLCQQAQSWFRACPYPMLVPLAGFLQPPGGPLRATLTGCHKGITAIAWSLEEKLLVVGTQDGAMVVWDVEEQQVVHVLMGHTAEVKCVRVFAQGTLAISASKDHTLRLWSLLSGQEKVTILDGGSQNPTEPQSWDLHVDERNNVVYSTSGARINMWNLETSKLVFCITGDVSDPWVCVALLAAQGLLLALSKGGQVSLWSSAMGKLQEKHQLSSIKEETPTCAVSIQSRARLVAGFSSGSIALVSAGEDRLLEKLPEAVGFLVVSEDDSLLVAGFGRFVRIFLADSQGFHRFMASDLEHEDMVETAVLGPENNLIITGSRDALIQVWSLSEQGTLLNVLEGVGAPVSLLVRGGTLVVSASRKSSSFKVWDLKSTKKLQSPTPFLDRTGLAAVSHHGSFVYFPKVGDKNKVTIWDLAEGEEQDCLDTSNEVRCLEVAEQAKLLFTGLVSGIVLVFPLNSRQDVLCIPPPEARKAVNCMSLSKSENRLAIAYDNIVLVLDISPGDPCPAIEGPTYTFYTQLPETIVSVAVLADYRVVYGMSDGSLFLYDCACSKVFPLEAHGSRVSCVEVSHSEQLAVSGAEDALLCLWDLQACRGMFEMSYENSCCRGVRCACFSRDDKHVFAGMEDRSVTAWSTVDGTLLAVQFVHAVINRIIPTSNGFMAPTSHGYLIRERFQCPSVRASQQDPLKNFKKAVWLVKTRQREELAAAEASQDAEPVAVEGKESKSNKRSQVCLIL

Target ORF information:

RefSeq Version	XM_030243606.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243606.1

ORF Insert Sequence:

ATGGTGGATC GGCTGGTAGA TGGCTGCCTG GATACCAATG CCCAGAGTCT TCTCAGTGGT 
CTTAAAGGGC GCATACTAGA TGCTCAGCCT GGAGCCCTAA AGTCCCACCA CCTGTCCTGG 
AGCCGAGACC TGGTCAACCC CAAGAACAAG GCTCACGCTC GTTACCTAAA GCAGTTGAGC 
GAGCAGTTCG TGGCCAGGAC CAACCATCAG GTCCTGGAGC AGCTTCGAGA GCTGGAGTTG 
GCCAGACAGG AGCTGGGTTG GCTCTACCAA GAGATCCGCC ACCACCTCTG GCAGAGCACA 
GAGTCTACCA AGGTCTTCTG CGGCCACCAG GAGCTCCTGG CCCAGCTCAG GCAGCAGCTC 
AGACAGGATG AGAGCCGGAC ACATACTCCC TTGGTTCTCT TTGGACCACC AGGCATCGGG 
AAGACCTCTC TCATGTGCAA GCTAGCCCAG CAGGTACCAG AGCTACTTGG CCACAAGACA 
GTGGTTGTCC TGAGGCTGCT AGGGACATCA AAGCTGAGCC TGGATGCCCG CAGCCTCCTC 
AGGAGCCTGT CCTTCCAAGT GTGCTTGGCA TATGGGCTGC CACTGCCCCC AGCCCAGGTC 
CTGGAGGCCC ACAGCAGGGT GGGCCATTTC TTCCATACTC TCCTCCACAC CGTGTCACAG 
AGGAACTTTG AGTCCCTGGT GTTGCTCCTG GACTCCGTGG ATGACCTGGA CTCCATATGC 
CATTCTCCGA GGGTCTCTTG GCTGCCCCTC AAGTGTCCTC CAAGGGTGCA CCTCATCCTC 
TCTACATGCT CAGGCCAGCA GGTTTTACAT AACCTGCAAC AGACCCTCAA GGACCCCAGC 
ACCTACTGGG AGGTGAAGGC CCTGTCTGGA AGTCAAGGCC AGGAGTTCAT ACAGCTGCTG 
CTGGCTGCAG AGAAGAGGAT GCTGAGCCCG GGTCAGAGAG ATGTCCTCTG GGCCAGCCTC 
CCAGAGTGTG GCCACCCAGG GAGGCTAAGG CTGGCCTTTG AGGAGGCCCG GAAGTGGGCC 
TCCTTCACTG TGCCAGTGCC CTTGGCCACT ACAGCAGAGG AAGCCACACA CCAGCTCTGT 
ATACGCCTGG AGGAGACACA TGGGGCACTG CTGGTGGCTC ATGTACTGGG CTATATTGTG 
TCTTCCAGGT ACGGTCTCTC AGAAGCAGAG CTGAAGGATG TGTTGTCTTT GGATGATGAA 
GTCTTGCAGG CTGTGTACCG GGACTGGACG CCACCTAGCA AAGAGCTGCT GCGCTTCCCG 
CCCCTGCTGT GGGTGCGGCT CCGCCGGGAC CTGGGACACT GCCTAGTCAG GAGGCCCGTG 
GATGGCTGCA TGCTACTGGC CATTGCACAC AGACAGCTGT CCCAGGTAAT CCAAGTTCGA 
TATCTGTCTG GGCCTGAGAG AGCCAAGAGG CATGGCGTGC TAGCTGAATT CTTCTCTGGA 
GCCTGGAGCC AGGGGATCAA GAAACTTATC ACCCTGCCGC TTGTAGGGAA GCCCCTGAAC 
CTGGACCGCA AGGTGGCCCC ACAGCCACTG TGGTTCTCAA GCACAGTGGC CAACCTGAGG 
AAGCTGACCG AACTGCCCTT CCATCTGCTG CATGCAGGCC GCTTGGAGGA GTTGAAGCAG 
GAGGTTCTGG GGAACATGAG TTGGATCTCA TGTCGGGGCA TCTCTGGAGG CATTGAGGTC 
CTGCTGGATG ATTTTGACAT GTGTGCCCCT CACATGAACT CTCCTGAGGT TGATCTGGTT 
AGAGAAGCCA TCCAGCTGTG TGGCCCTGCT GTGGAGCTCC GGGGCCTGGA GAAGAGCGTC 
CTATACACCG AGCTCCTGGC CAGACTCCTT TTCTTTGCTG CATCACATCC AGCAATGATC 
GGACAACTGT GCCAACAAGC CCAGAGCTGG TTCCGTGCCT GCCCCTACCC AATGCTGGTG 
CCCCTGGCAG GATTCCTGCA GCCACCTGGT GGACCTCTTC GAGCCACTCT TACTGGGTGT 
CACAAAGGCA TCACCGCCAT AGCATGGAGC CTGGAGGAGA AACTCCTGGT GGTGGGCACC 
CAGGATGGTG CCATGGTCGT GTGGGACGTG GAGGAACAAC AGGTGGTACA CGTTCTAATG 
GGACACACAG CTGAAGTGAA GTGTGTGCGA GTGTTTGCCC AAGGGACTCT TGCCATCTCT 
GCATCAAAGG ACCATACCCT GCGTCTGTGG AGTCTGCTTT CCGGCCAGGA GAAAGTCACC 
ATTTTGGATG GAGGGTCACA AAATCCCACA GAGCCCCAGA GTTGGGACCT TCATGTGGAT 
GAGAGAAACA ATGTTGTATA CTCGACATCT GGTGCAAGGA TCAACATGTG GAATCTGGAA 
ACTTCGAAGC TGGTTTTCTG TATCACAGGA GATGTGTCTG ATCCCTGGGT CTGTGTGGCT 
TTGTTGGCTG CCCAGGGTTT GCTGCTTGCC CTGTCCAAGG GTGGCCAGGT CAGTCTGTGG 
AGCTCAGCCA TGGGAAAACT GCAGGAGAAA CACCAGTTAT CCAGCATCAA AGAAGAGACA 
CCCACCTGTG CCGTCTCCAT CCAGTCCCGG GCAAGGCTGG TCGCTGGCTT CAGCAGTGGC 
TCCATTGCCC TGGTCTCCGC TGGAGAAGAC AGGCTGCTGG AGAAGCTCCC AGAAGCTGTG 
GGGTTCCTGG TGGTTTCTGA AGACGATTCC CTTCTTGTGG CAGGCTTTGG AAGATTTGTG 
CGGATTTTCT TGGCTGACTC GCAAGGCTTC CACCGGTTCA TGGCTTCGGA TCTGGAACAC 
GAGGACATGG TGGAGACAGC AGTCTTGGGT CCTGAGAACA ATCTGATCAT CACAGGGTCC 
CGGGATGCAC TTATCCAGGT GTGGAGTTTG TCAGAACAGG GGACCCTGCT GAATGTCCTT 
GAGGGCGTGG GCGCCCCTGT GAGTCTGCTG GTCCGGGGTG GGACTCTGGT GGTGTCTGCA 
TCCCGGAAGT CTTCATCTTT CAAGGTCTGG GATCTCAAGT CCACTAAGAA GCTGCAGTCT 
CCGACTCCAT TTCTGGACCG CACCGGCCTC GCTGCAGTGT CCCACCATGG CAGCTTTGTC 
TACTTCCCTA AGGTTGGGGA CAAAAACAAA GTCACAATTT GGGACTTGGC AGAAGGTGAG 
GAGCAAGATT GCCTGGACAC GTCCAATGAG GTCCGCTGTC TGGAGGTGGC CGAGCAGGCC 
AAGCTCCTCT TCACAGGCCT GGTTTCTGGG ATCGTGCTTG TGTTCCCCCT GAATTCCAGG 
CAAGATGTAC TGTGCATCCC CCCACCTGAG GCACGCAAAG CTGTCAACTG CATGTCCCTG 
AGCAAGAGTG AGAATCGTCT GGCCATTGCC TATGACAACA TTGTCCTGGT GCTTGACATC 
AGCCCTGGGG ACCCATGTCC GGCCATTGAA GGCCCCACCT ACACCTTTTA CACCCAGCTG 
CCTGAGACCA TAGTGAGTGT GGCTGTCCTG GCTGACTATC GTGTGGTCTA CGGCATGAGC 
GATGGCAGCC TCTTCCTGTA CGACTGTGCA TGCTCTAAAG TGTTCCCTCT GGAGGCCCAT 
GGGAGCAGGG TGAGCTGTGT AGAGGTCAGC CACAGTGAGC AGCTAGCAGT GAGCGGGGCT 
GAAGATGCAC TGCTATGCCT CTGGGACCTG CAGGCTTGCA GGGGGATGTT CGAGATGAGC 
TATGAGAACT CCTGCTGCAG AGGGGTGCGG TGTGCCTGCT TCTCCAGGGA TGACAAGCAT 
GTATTTGCAG GCATGGAGGA CCGCTCTGTC ACTGCCTGGA GCACGGTGGA TGGCACTCTT 
TTGGCTGTCC AGTTTGTTCA TGCTGTGATC AACCGAATCA TCCCTACCTC CAATGGCTTC 
ATGGCCCCCA CAAGCCACGG CTATCTCATC CGTGAGCGCT TCCAGTGCCC GTCAGTCAGA 
GCCTCCCAGC AAGACCCTCT CAAGAACTTC AAGAAGGCAG TGTGGCTGGT AAAGACCAGG 
CAGAGGGAAG AGCTGGCTGC AGCAGAAGCC TCCCAGGATG CAGAACCAGT GGCCGTAGAG 
GGAAAGGAAT CAAAGTCAAA TAAACGCTCA CAGGTCTGCC TCATACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nwd1 ( XM_006531091.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531154.1 Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X6, mRNA.

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CloneID	OMu41534
Clone ID Related Accession (Same CDS sequence)	XM_006531091.3
Accession Version	XM_006531091.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531091.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAG AGCGAGAAGC CCTGCAGTGC ACAGCCTACC CTGAGGTTCA GAGCTTCTGC 
CAAAGGCACG GCTTGGCGTT TGAGGTGGTT GATCTGAGGT GGGGTATTCC GAATACGCAG 
GCCACTGACT ACCTGACCAC GGAACTCTGC TTAGAAGAAC TGGAGCGCTG TCAGAAAACA 
TCCATAGGAC CAGCTTTTGT GGCCCTCCTG GGTGACCAGT ACGGCCCCTG CCCGGTTCCC 
CGGAGGATTG AGGAAAAGGA GTGGGAGGCA CTAAGAGCTC AGCTGACCTC CAGGCCTCGA 
GATCTGGAGC TGGTGACCAG ACATTTCCAG AGAGACGACA ACACCATTCC TCCAACCTAC 
GTCTTGCAGC CTTCAGGCTC ACTGGTGGTC CCAGGGCCTG AGGAGGCCAC CTTAACCTCT 
GTCCTGAGAG GTGGAGCCCA GGAGGCCTGG AGGCTAGGCC TCATCAGCCA GGAGCAATGG 
ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG GATTTGGACA GAACCTGGAT TTTGTGGAGG 
TACGGTCTCT CAGAAGCAGA GCTGAAGGAT GTGTTGTCTT TGGATGATGA AGTCTTGCAG 
GCTGTGTACC GGGACTGGAC GCCACCTAGC AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG 
TGGGTGCGGC TCCGCCGGGA CCTGGGACAC TGCCTAGTCA GGAGGCCCGT GGATGGCTGC 
ATGCTACTGG CCATTGCACA CAGACAGCTG TCCCAGGTAA TCCAAGTTCG ATATCTGTCT 
GGGCCTGAGA GAGCCAAGAG GCATGGCGTG CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC 
CAGGGGATCA AGAAACTTAT CACCCTGCCG CTTGTAGGGA AGCCCCTGAA CCTGGACCGC 
AAGGTGGCCC CACAGCCACT GTGGTTCTCA AGCACAGTGG CCAACCTGAG GAAGCTGACC 
GAACTGCCCT TCCATCTGCT GCATGCAGGC CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG 
GGGAACATGA GTTGGATCTC ATGTCGGGGC ATCTCTGGAG GCATTGAGGT CCTGCTGGAT 
GATTTTGACA TGTGTGCCCC TCACATGAAC TCTCCTGAGG TTGATCTGGT TAGAGAAGCC 
ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC CGGGGCCTGG AGAAGAGCGT CCTATACACC 
GAGCTCCTGG CCAGACTCCT TTTCTTTGCT GCATCACATC CAGCAATGAT CGGACAACTG 
TGCCAACAAG CCCAGAGCTG GTTCCGTGCC TGCCCCTACC CAATGCTGGT GCCCCTGGCA 
GGATTCCTGC AGCCACCTGG TGGACCTCTT CGAGCCACTC TTACTGGGTG TCACAAAGGC 
ATCACCGCCA TAGCATGGAG CCTGGAGGAG AAACTCCTGG TGGTGGGCAC CCAGGATGGT 
GCCATGGTCG TGTGGGACGT GGAGGAACAA CAGGTGGTAC ACGTTCTAAT GGGACACACA 
GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC CAAGGGACTC TTGCCATCTC TGCATCAAAG 
GACCATACCC TGCGTCTGTG GAGTCTGCTT TCCGGCCAGG AGAAAGTCAC CATTTTGGAT 
GGAGGGTCAC AAAATCCCAC AGAGCCCCAG AGTTGGGACC TTCATGTGGA TGAGAGAAAC 
AATGTTGTAT ACTCGACATC TGGTGCAAGG ATCAACATGT GGAATCTGGA AACTTCGAAG 
CTGGTTTTCT GTATCACAGG AGATGTGTCT GATCCCTGGG TCTGTGTGGC TTTGTTGGCT 
GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC 
ATGGGAAAAC TGCAGGAGAA ACACCAGTTA TCCAGCATCA AAGAAGAGAC ACCCACCTGT 
GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG GTCGCTGGCT TCAGCAGTGG CTCCATTGCC 
CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG 
GTGGTTTCTG AAGACGATTC CCTTCTTGTG GCAGGCTTTG GAAGATTTGT GCGGATTTTC 
TTGGCTGACT CGCAAGGCTT CCACCGGTTC ATGGCTTCGG ATCTGGAACA CGAGGACATG 
GTGGAGACAG CAGTCTTGGG TCCTGAGAAC AATCTGATCA TCACAGGGTC CCGGGATGCA 
CTTATCCAGG TGTGGAGTTT GTCAGAACAG GGGACCCTGC TGAATGTCCT TGAGGGCGTG 
GGCGCCCCTG TGAGTCTGCT GGTCCGGGGT GGGACTCTGG TGGTGTCTGC ATCCCGGAAG 
TCTTCATCTT TCAAGGTCTG GGATCTCAAG TCCACTAAGA AGCTGCAGTC TCCGACTCCA 
TTTCTGGACC GCACCGGCCT CGCTGCAGTG TCCCACCATG GCAGCTTTGT CTACTTCCCT 
AAGGTGAGGA GCAAGATTGC CTGGACACGT CCAATGAGGT CCGCTGTCTG GAGGTGGCCG 
AGCAGGCCAA GCTCCTCTTC ACAGGCCTGG TTTCTGGGAT CGTGCTTGTG TTCCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531154.1
CDS	788..4627
Translation	MDTEREALQCTAYPEVQSFCQRHGLAFEVVDLRWGIPNTQATDYLTTELCLEELERCQKTSIGPAFVALLGDQYGPCPVPRRIEEKEWEALRAQLTSRPRDLELVTRHFQRDDNTIPPTYVLQPSGSLVVPGPEEATLTSVLRGGAQEAWRLGLISQEQWMCYHRSVIEWEIELGLLSSARGDQGATVFLRDVQDLNKHILDDCSLKMVDRLVDGCLDTNAQSLLSGLKGRILDAQPGALKSHHLSWSRDLVNPKNKAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPGIGKTSLMCKLAQQVPELLGHKTVVVLRLLGTSKLSLDARSLLRSLSFQVCLAYGLPLPPAQVLEAHSRVGHFFHTLLHTVSQRNFESLVLLLDSVDDLDSICHSPRVSWLPLKCPPRVHLILSTCSGQQVLHNLQQTLKDPSTYWEVKALSGSQGQEFIQLLLAAEKRMLSPGQRDVLWASLPECGHPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCIRLEETHGALLVAHVLGYIVSSRDLDRTWILWRYGLSEAELKDVLSLDDEVLQAVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLVRRPVDGCMLLAIAHRQLSQVIQVRYLSGPERAKRHGVLAEFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSSTVANLRKLTELPFHLLHAGRLEELKQEVLGNMSWISCRGISGGIEVLLDDFDMCAPHMNSPEVDLVREAIQLCGPAVELRGLEKSVLYTELLARLLFFAASHPAMIGQLCQQAQSWFRACPYPMLVPLAGFLQPPGGPLRATLTGCHKGITAIAWSLEEKLLVVGTQDGAMVVWDVEEQQVVHVLMGHTAEVKCVRVFAQGTLAISASKDHTLRLWSLLSGQEKVTILDGGSQNPTEPQSWDLHVDERNNVVYSTSGARINMWNLETSKLVFCITGDVSDPWVCVALLAAQGLLLALSKGGQVSLWSSAMGKLQEKHQLSSIKEETPTCAVSIQSRARLVAGFSSGSIALVSAGEDRLLEKLPEAVGFLVVSEDDSLLVAGFGRFVRIFLADSQGFHRFMASDLEHEDMVETAVLGPENNLIITGSRDALIQVWSLSEQGTLLNVLEGVGAPVSLLVRGGTLVVSASRKSSSFKVWDLKSTKKLQSPTPFLDRTGLAAVSHHGSFVYFPKVRSKIAWTRPMRSAVWRWPSRPSSSSQAWFLGSCLCSP

Target ORF information:

RefSeq Version	XM_006531091.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531091.3

ORF Insert Sequence:

ATGGACACAG AGCGAGAAGC CCTGCAGTGC ACAGCCTACC CTGAGGTTCA GAGCTTCTGC 
CAAAGGCACG GCTTGGCGTT TGAGGTGGTT GATCTGAGGT GGGGTATTCC GAATACGCAG 
GCCACTGACT ACCTGACCAC GGAACTCTGC TTAGAAGAAC TGGAGCGCTG TCAGAAAACA 
TCCATAGGAC CAGCTTTTGT GGCCCTCCTG GGTGACCAGT ACGGCCCCTG CCCGGTTCCC 
CGGAGGATTG AGGAAAAGGA GTGGGAGGCA CTAAGAGCTC AGCTGACCTC CAGGCCTCGA 
GATCTGGAGC TGGTGACCAG ACATTTCCAG AGAGACGACA ACACCATTCC TCCAACCTAC 
GTCTTGCAGC CTTCAGGCTC ACTGGTGGTC CCAGGGCCTG AGGAGGCCAC CTTAACCTCT 
GTCCTGAGAG GTGGAGCCCA GGAGGCCTGG AGGCTAGGCC TCATCAGCCA GGAGCAATGG 
ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG GATTTGGACA GAACCTGGAT TTTGTGGAGG 
TACGGTCTCT CAGAAGCAGA GCTGAAGGAT GTGTTGTCTT TGGATGATGA AGTCTTGCAG 
GCTGTGTACC GGGACTGGAC GCCACCTAGC AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG 
TGGGTGCGGC TCCGCCGGGA CCTGGGACAC TGCCTAGTCA GGAGGCCCGT GGATGGCTGC 
ATGCTACTGG CCATTGCACA CAGACAGCTG TCCCAGGTAA TCCAAGTTCG ATATCTGTCT 
GGGCCTGAGA GAGCCAAGAG GCATGGCGTG CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC 
CAGGGGATCA AGAAACTTAT CACCCTGCCG CTTGTAGGGA AGCCCCTGAA CCTGGACCGC 
AAGGTGGCCC CACAGCCACT GTGGTTCTCA AGCACAGTGG CCAACCTGAG GAAGCTGACC 
GAACTGCCCT TCCATCTGCT GCATGCAGGC CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG 
GGGAACATGA GTTGGATCTC ATGTCGGGGC ATCTCTGGAG GCATTGAGGT CCTGCTGGAT 
GATTTTGACA TGTGTGCCCC TCACATGAAC TCTCCTGAGG TTGATCTGGT TAGAGAAGCC 
ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC CGGGGCCTGG AGAAGAGCGT CCTATACACC 
GAGCTCCTGG CCAGACTCCT TTTCTTTGCT GCATCACATC CAGCAATGAT CGGACAACTG 
TGCCAACAAG CCCAGAGCTG GTTCCGTGCC TGCCCCTACC CAATGCTGGT GCCCCTGGCA 
GGATTCCTGC AGCCACCTGG TGGACCTCTT CGAGCCACTC TTACTGGGTG TCACAAAGGC 
ATCACCGCCA TAGCATGGAG CCTGGAGGAG AAACTCCTGG TGGTGGGCAC CCAGGATGGT 
GCCATGGTCG TGTGGGACGT GGAGGAACAA CAGGTGGTAC ACGTTCTAAT GGGACACACA 
GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC CAAGGGACTC TTGCCATCTC TGCATCAAAG 
GACCATACCC TGCGTCTGTG GAGTCTGCTT TCCGGCCAGG AGAAAGTCAC CATTTTGGAT 
GGAGGGTCAC AAAATCCCAC AGAGCCCCAG AGTTGGGACC TTCATGTGGA TGAGAGAAAC 
AATGTTGTAT ACTCGACATC TGGTGCAAGG ATCAACATGT GGAATCTGGA AACTTCGAAG 
CTGGTTTTCT GTATCACAGG AGATGTGTCT GATCCCTGGG TCTGTGTGGC TTTGTTGGCT 
GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC 
ATGGGAAAAC TGCAGGAGAA ACACCAGTTA TCCAGCATCA AAGAAGAGAC ACCCACCTGT 
GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG GTCGCTGGCT TCAGCAGTGG CTCCATTGCC 
CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG 
GTGGTTTCTG AAGACGATTC CCTTCTTGTG GCAGGCTTTG GAAGATTTGT GCGGATTTTC 
TTGGCTGACT CGCAAGGCTT CCACCGGTTC ATGGCTTCGG ATCTGGAACA CGAGGACATG 
GTGGAGACAG CAGTCTTGGG TCCTGAGAAC AATCTGATCA TCACAGGGTC CCGGGATGCA 
CTTATCCAGG TGTGGAGTTT GTCAGAACAG GGGACCCTGC TGAATGTCCT TGAGGGCGTG 
GGCGCCCCTG TGAGTCTGCT GGTCCGGGGT GGGACTCTGG TGGTGTCTGC ATCCCGGAAG 
TCTTCATCTT TCAAGGTCTG GGATCTCAAG TCCACTAAGA AGCTGCAGTC TCCGACTCCA 
TTTCTGGACC GCACCGGCCT CGCTGCAGTG TCCCACCATG GCAGCTTTGT CTACTTCCCT 
AAGGTGAGGA GCAAGATTGC CTGGACACGT CCAATGAGGT CCGCTGTCTG GAGGTGGCCG 
AGCAGGCCAA GCTCCTCTTC ACAGGCCTGG TTTCTGGGAT CGTGCTTGTG TTCCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nwd1 ( XM_006531092.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531155.1 Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu41535
Clone ID Related Accession (Same CDS sequence)	XM_006531092.2
Accession Version	XM_006531092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006531092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAG AGCGAGAAGC CCTGCAGTGC ACAGCCTACC CTGAGGTTCA GAGCTTCTGC 
CAAAGGCACG GCTTGGCGTT TGAGGTGGTT GATCTGAGGT GGGGTATTCC GAATACGCAG 
GCCACTGACT ACCTGACCAC GGAACTCTGC TTAGAAGAAC TGGAGCGCTG TCAGAAAACA 
TCCATAGGAC CAGCTTTTGT GGCCCTCCTG GGTGACCAGT ACGGCCCCTG CCCGGTTCCC 
CGGAGGATTG AGGAAAAGGA GTGGGAGGCA CTAAGAGCTC AGCTGACCTC CAGGCCTCGA 
GATCTGGAGC TGGTGACCAG ACATTTCCAG AGAGACGACA ACACCATTCC TCCAACCTAC 
GTCTTGCAGC CTTCAGGCTC ACTGGTGGTC CCAGGGCCTG AGGAGGCCAC CTTAACCTCT 
GTCCTGAGAG GTGGAGCCCA GGAGGCCTGG AGGCTAGGCC TCATCAGCCA GGAGCAATGG 
ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG TACGGTCTCT CAGAAGCAGA GCTGAAGGAT 
GTGTTGTCTT TGGATGATGA AGTCTTGCAG GCTGTGTACC GGGACTGGAC GCCACCTAGC 
AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TCCGCCGGGA CCTGGGACAC 
TGCCTAGTCA GGAGGCCCGT GGATGGCTGC ATGCTACTGG CCATTGCACA CAGACAGCTG 
TCCCAGGTAA TCCAAGTTCG ATATCTGTCT GGGCCTGAGA GAGCCAAGAG GCATGGCGTG 
CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC CAGGGGATCA AGAAACTTAT CACCCTGCCG 
CTTGTAGGGA AGCCCCTGAA CCTGGACCGC AAGGTGGCCC CACAGCCACT GTGGTTCTCA 
AGCACAGTGG CCAACCTGAG GAAGCTGACC GAACTGCCCT TCCATCTGCT GCATGCAGGC 
CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG GGGAACATGA GTTGGATCTC ATGTCGGGGC 
ATCTCTGGAG GCATTGAGGT CCTGCTGGAT GATTTTGACA TGTGTGCCCC TCACATGAAC 
TCTCCTGAGG TTGATCTGGT TAGAGAAGCC ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC 
CGGGGCCTGG AGAAGAGCGT CCTATACACC GAGCTCCTGG CCAGACTCCT TTTCTTTGCT 
GCATCACATC CAGCAATGAT CGGACAACTG TGCCAACAAG CCCAGAGCTG GTTCCGTGCC 
TGCCCCTACC CAATGCTGGT GCCCCTGGCA GGATTCCTGC AGCCACCTGG TGGACCTCTT 
CGAGCCACTC TTACTGGGTG TCACAAAGGC ATCACCGCCA TAGCATGGAG CCTGGAGGAG 
AAACTCCTGG TGGTGGGCAC CCAGGATGGT GCCATGGTCG TGTGGGACGT GGAGGAACAA 
CAGGTGGTAC ACGTTCTAAT GGGACACACA GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC 
CAAGGGACTC TTGCCATCTC TGCATCAAAG GACCATACCC TGCGTCTGTG GAGTCTGCTT 
TCCGGCCAGG AGAAAGTCAC CATTTTGGAT GGAGGGTCAC AAAATCCCAC AGAGCCCCAG 
AGTTGGGACC TTCATGTGGA TGAGAGAAAC AATGTTGTAT ACTCGACATC TGGTGCAAGG 
ATCAACATGT GGAATCTGGA AACTTCGAAG CTGGTTTTCT GTATCACAGG AGATGTGTCT 
GATCCCTGGG TCTGTGTGGC TTTGTTGGCT GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG 
GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC ATGGGAAAAC TGCAGGAGAA ACACCAGTTA 
TCCAGCATCA AAGAAGAGAC ACCCACCTGT GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG 
GTCGCTGGCT TCAGCAGTGG CTCCATTGCC CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG 
GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG GTGGTTTCTG AAGACGATTC CCTTCTTGTG 
GCAGGCTTTG GAAGATTTGT GCGGATTTTC TTGGCTGACT CGCAAGGCTT CCACCGGTTC 
ATGGCTTCGG ATCTGGAACA CGAGGACATG GTGGAGACAG CAGTCTTGGG TCCTGAGAAC 
AATCTGATCA TCACAGGGTC CCGGGATGCA CTTATCCAGG TGTGGAGTTT GTCAGAACAG 
GGGACCCTGC TGAATGTCCT TGAGGGCGTG GGCGCCCCTG TGAGTCTGCT GGTCCGGGGT 
GGGACTCTGG TGGTGTCTGC ATCCCGGAAG TCTTCATCTT TCAAGGTCTG GGATCTCAAG 
TCCACTAAGA AGCTGCAGTC TCCGACTCCA TTTCTGGACC GCACCGGCCT CGCTGCAGTG 
TCCCACCATG GCAGCTTTGT CTACTTCCCT AAGGTGAGGA GCAAGATTGC CTGGACACGT 
CCAATGAGGT CCGCTGTCTG GAGGTGGCCG AGCAGGCCAA GCTCCTCTTC ACAGGCCTGG 
TTTCTGGGAT CGTGCTTGTG TTCCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531155.1
CDS	788..4597
Translation	MDTEREALQCTAYPEVQSFCQRHGLAFEVVDLRWGIPNTQATDYLTTELCLEELERCQKTSIGPAFVALLGDQYGPCPVPRRIEEKEWEALRAQLTSRPRDLELVTRHFQRDDNTIPPTYVLQPSGSLVVPGPEEATLTSVLRGGAQEAWRLGLISQEQWMCYHRSVIEWEIELGLLSSARGDQGATVFLRDVQDLNKHILDDCSLKMVDRLVDGCLDTNAQSLLSGLKGRILDAQPGALKSHHLSWSRDLVNPKNKAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPGIGKTSLMCKLAQQVPELLGHKTVVVLRLLGTSKLSLDARSLLRSLSFQVCLAYGLPLPPAQVLEAHSRVGHFFHTLLHTVSQRNFESLVLLLDSVDDLDSICHSPRVSWLPLKCPPRVHLILSTCSGQQVLHNLQQTLKDPSTYWEVKALSGSQGQEFIQLLLAAEKRMLSPGQRDVLWASLPECGHPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCIRLEETHGALLVAHVLGYIVSSRYGLSEAELKDVLSLDDEVLQAVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLVRRPVDGCMLLAIAHRQLSQVIQVRYLSGPERAKRHGVLAEFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSSTVANLRKLTELPFHLLHAGRLEELKQEVLGNMSWISCRGISGGIEVLLDDFDMCAPHMNSPEVDLVREAIQLCGPAVELRGLEKSVLYTELLARLLFFAASHPAMIGQLCQQAQSWFRACPYPMLVPLAGFLQPPGGPLRATLTGCHKGITAIAWSLEEKLLVVGTQDGAMVVWDVEEQQVVHVLMGHTAEVKCVRVFAQGTLAISASKDHTLRLWSLLSGQEKVTILDGGSQNPTEPQSWDLHVDERNNVVYSTSGARINMWNLETSKLVFCITGDVSDPWVCVALLAAQGLLLALSKGGQVSLWSSAMGKLQEKHQLSSIKEETPTCAVSIQSRARLVAGFSSGSIALVSAGEDRLLEKLPEAVGFLVVSEDDSLLVAGFGRFVRIFLADSQGFHRFMASDLEHEDMVETAVLGPENNLIITGSRDALIQVWSLSEQGTLLNVLEGVGAPVSLLVRGGTLVVSASRKSSSFKVWDLKSTKKLQSPTPFLDRTGLAAVSHHGSFVYFPKVRSKIAWTRPMRSAVWRWPSRPSSSSQAWFLGSCLCSP

Target ORF information:

RefSeq Version	XM_006531092.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531092.2

ORF Insert Sequence:

ATGGACACAG AGCGAGAAGC CCTGCAGTGC ACAGCCTACC CTGAGGTTCA GAGCTTCTGC 
CAAAGGCACG GCTTGGCGTT TGAGGTGGTT GATCTGAGGT GGGGTATTCC GAATACGCAG 
GCCACTGACT ACCTGACCAC GGAACTCTGC TTAGAAGAAC TGGAGCGCTG TCAGAAAACA 
TCCATAGGAC CAGCTTTTGT GGCCCTCCTG GGTGACCAGT ACGGCCCCTG CCCGGTTCCC 
CGGAGGATTG AGGAAAAGGA GTGGGAGGCA CTAAGAGCTC AGCTGACCTC CAGGCCTCGA 
GATCTGGAGC TGGTGACCAG ACATTTCCAG AGAGACGACA ACACCATTCC TCCAACCTAC 
GTCTTGCAGC CTTCAGGCTC ACTGGTGGTC CCAGGGCCTG AGGAGGCCAC CTTAACCTCT 
GTCCTGAGAG GTGGAGCCCA GGAGGCCTGG AGGCTAGGCC TCATCAGCCA GGAGCAATGG 
ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG TACGGTCTCT CAGAAGCAGA GCTGAAGGAT 
GTGTTGTCTT TGGATGATGA AGTCTTGCAG GCTGTGTACC GGGACTGGAC GCCACCTAGC 
AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TCCGCCGGGA CCTGGGACAC 
TGCCTAGTCA GGAGGCCCGT GGATGGCTGC ATGCTACTGG CCATTGCACA CAGACAGCTG 
TCCCAGGTAA TCCAAGTTCG ATATCTGTCT GGGCCTGAGA GAGCCAAGAG GCATGGCGTG 
CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC CAGGGGATCA AGAAACTTAT CACCCTGCCG 
CTTGTAGGGA AGCCCCTGAA CCTGGACCGC AAGGTGGCCC CACAGCCACT GTGGTTCTCA 
AGCACAGTGG CCAACCTGAG GAAGCTGACC GAACTGCCCT TCCATCTGCT GCATGCAGGC 
CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG GGGAACATGA GTTGGATCTC ATGTCGGGGC 
ATCTCTGGAG GCATTGAGGT CCTGCTGGAT GATTTTGACA TGTGTGCCCC TCACATGAAC 
TCTCCTGAGG TTGATCTGGT TAGAGAAGCC ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC 
CGGGGCCTGG AGAAGAGCGT CCTATACACC GAGCTCCTGG CCAGACTCCT TTTCTTTGCT 
GCATCACATC CAGCAATGAT CGGACAACTG TGCCAACAAG CCCAGAGCTG GTTCCGTGCC 
TGCCCCTACC CAATGCTGGT GCCCCTGGCA GGATTCCTGC AGCCACCTGG TGGACCTCTT 
CGAGCCACTC TTACTGGGTG TCACAAAGGC ATCACCGCCA TAGCATGGAG CCTGGAGGAG 
AAACTCCTGG TGGTGGGCAC CCAGGATGGT GCCATGGTCG TGTGGGACGT GGAGGAACAA 
CAGGTGGTAC ACGTTCTAAT GGGACACACA GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC 
CAAGGGACTC TTGCCATCTC TGCATCAAAG GACCATACCC TGCGTCTGTG GAGTCTGCTT 
TCCGGCCAGG AGAAAGTCAC CATTTTGGAT GGAGGGTCAC AAAATCCCAC AGAGCCCCAG 
AGTTGGGACC TTCATGTGGA TGAGAGAAAC AATGTTGTAT ACTCGACATC TGGTGCAAGG 
ATCAACATGT GGAATCTGGA AACTTCGAAG CTGGTTTTCT GTATCACAGG AGATGTGTCT 
GATCCCTGGG TCTGTGTGGC TTTGTTGGCT GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG 
GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC ATGGGAAAAC TGCAGGAGAA ACACCAGTTA 
TCCAGCATCA AAGAAGAGAC ACCCACCTGT GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG 
GTCGCTGGCT TCAGCAGTGG CTCCATTGCC CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG 
GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG GTGGTTTCTG AAGACGATTC CCTTCTTGTG 
GCAGGCTTTG GAAGATTTGT GCGGATTTTC TTGGCTGACT CGCAAGGCTT CCACCGGTTC 
ATGGCTTCGG ATCTGGAACA CGAGGACATG GTGGAGACAG CAGTCTTGGG TCCTGAGAAC 
AATCTGATCA TCACAGGGTC CCGGGATGCA CTTATCCAGG TGTGGAGTTT GTCAGAACAG 
GGGACCCTGC TGAATGTCCT TGAGGGCGTG GGCGCCCCTG TGAGTCTGCT GGTCCGGGGT 
GGGACTCTGG TGGTGTCTGC ATCCCGGAAG TCTTCATCTT TCAAGGTCTG GGATCTCAAG 
TCCACTAAGA AGCTGCAGTC TCCGACTCCA TTTCTGGACC GCACCGGCCT CGCTGCAGTG 
TCCCACCATG GCAGCTTTGT CTACTTCCCT AAGGTGAGGA GCAAGATTGC CTGGACACGT 
CCAATGAGGT CCGCTGTCTG GAGGTGGCCG AGCAGGCCAA GCTCCTCTTC ACAGGCCTGG 
TTTCTGGGAT CGTGCTTGTG TTCCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nwd1 ( XM_006531093.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531156.1 Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu41536
Clone ID Related Accession (Same CDS sequence)	XM_006531093.2
Accession Version	XM_006531093.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006531093.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATC GGCTGGTAGA TGGCTGCCTG GATACCAATG CCCAGAGTCT TCTCAGTGGT 
CTTAAAGGGC GCATACTAGA TGCTCAGCCT GGAGCCCTAA AGTCCCACCA CCTGTCCTGG 
AGCCGAGACC TGGTCAACCC CAAGAACAAG GCTCACGCTC GTTACCTAAA GCAGTTGAGC 
GAGCAGTTCG TGGCCAGGAC CAACCATCAG GTCCTGGAGC AGCTTCGAGA GCTGGAGTTG 
GCCAGACAGG AGCTGGGTTG GCTCTACCAA GAGATCCGCC ACCACCTCTG GCAGAGCACA 
GAGTCTACCA AGGTCTTCTG CGGCCACCAG GAGCTCCTGG CCCAGCTCAG GCAGCAGCTC 
AGACAGGATG AGAGCCGGAC ACATACTCCC TTGGTTCTCT TTGGACCACC AGGCATCGGG 
AAGACCTCTC TCATGTGCAA GCTAGCCCAG CAGGTACCAG AGCTACTTGG CCACAAGACA 
GTGGTTGTCC TGAGGCTGCT AGGGACATCA AAGCTGAGCC TGGATGCCCG CAGCCTCCTC 
AGGAGCCTGT CCTTCCAAGT GTGCTTGGCA TATGGGCTGC CACTGCCCCC AGCCCAGGTC 
CTGGAGGCCC ACAGCAGGGT GGGCCATTTC TTCCATACTC TCCTCCACAC CGTGTCACAG 
AGGAACTTTG AGTCCCTGGT GTTGCTCCTG GACTCCGTGG ATGACCTGGA CTCCATATGC 
CATTCTCCGA GGGTCTCTTG GCTGCCCCTC AAGTGTCCTC CAAGGGTGCA CCTCATCCTC 
TCTACATGCT CAGGCCAGCA GGTTTTACAT AACCTGCAAC AGACCCTCAA GGACCCCAGC 
ACCTACTGGG AGGTGAAGGC CCTGTCTGGA AGTCAAGGCC AGGAGTTCAT ACAGCTGCTG 
CTGGCTGCAG AGAAGAGGAT GCTGAGCCCG GGTCAGAGAG ATGTCCTCTG GGCCAGCCTC 
CCAGAGTGTG GCCACCCAGG GAGGCTAAGG CTGGCCTTTG AGGAGGCCCG GAAGTGGGCC 
TCCTTCACTG TGCCAGTGCC CTTGGCCACT ACAGCAGAGG AAGCCACACA CCAGCTCTGT 
ATACGCCTGG AGGAGACACA TGGGGCACTG CTGGTGGCTC ATGTACTGGG CTATATTGTG 
TCTTCCAGGT ACGGTCTCTC AGAAGCAGAG CTGAAGGATG TGTTGTCTTT GGATGATGAA 
GTCTTGCAGG CTGTGTACCG GGACTGGACG CCACCTAGCA AAGAGCTGCT GCGCTTCCCG 
CCCCTGCTGT GGGTGCGGCT CCGCCGGGAC CTGGGACACT GCCTAGTCAG GAGGCCCGTG 
GATGGCTGCA TGCTACTGGC CATTGCACAC AGACAGCTGT CCCAGGTAAT CCAAGTTCGA 
TATCTGTCTG GGCCTGAGAG AGCCAAGAGG CATGGCGTGC TAGCTGAATT CTTCTCTGGA 
GCCTGGAGCC AGGGGATCAA GAAACTTATC ACCCTGCCGC TTGTAGGGAA GCCCCTGAAC 
CTGGACCGCA AGGTGGCCCC ACAGCCACTG TGGTTCTCAA GCACAGTGGC CAACCTGAGG 
AAGCTGACCG AACTGCCCTT CCATCTGCTG CATGCAGGCC GCTTGGAGGA GTTGAAGCAG 
GAGGTTCTGG GGAACATGAG TTGGATCTCA TGTCGGGGCA TCTCTGGAGG CATTGAGGTC 
CTGCTGGATG ATTTTGACAT GTGTGCCCCT CACATGAACT CTCCTGAGGT TGATCTGGTT 
AGAGAAGCCA TCCAGCTGTG TGGCCCTGCT GTGGAGCTCC GGGGCCTGGA GAAGAGCGTC 
CTATACACCG AGCTCCTGGC CAGACTCCTT TTCTTTGCTG CATCACATCC AGCAATGATC 
GGACAACTGT GCCAACAAGC CCAGAGCTGG TTCCGTGCCT GCCCCTACCC AATGCTGGTG 
CCCCTGGCAG GATTCCTGCA GCCACCTGGT GGACCTCTTC GAGCCACTCT TACTGGGTGT 
CACAAAGGCA TCACCGCCAT AGCATGGAGC CTGGAGGAGA AACTCCTGGT GGTGGGCACC 
CAGGATGGTG CCATGGTCGT GTGGGACGTG GAGGAACAAC AGGTGGTACA CGTTCTAATG 
GGACACACAG CTGAAGTGAA GTGTGTGCGA GTGTTTGCCC AAGGGACTCT TGCCATCTCT 
GCATCAAAGG ACCATACCCT GCGTCTGTGG AGTCTGCTTT CCGGCCAGGA GAAAGTCACC 
ATTTTGGATG GAGGGTCACA AAATCCCACA GAGCCCCAGA GTTGGGACCT TCATGTGGAT 
GAGAGAAACA ATGTTGTATA CTCGACATCT GGTGCAAGGA TCAACATGTG GAATCTGGAA 
ACTTCGAAGC TGGTTTTCTG TATCACAGGA GATGTGTCTG ATCCCTGGGT CTGTGTGGCT 
TTGTTGGCTG CCCAGGGTTT GCTGCTTGCC CTGTCCAAGG GTGGCCAGGT CAGTCTGTGG 
AGCTCAGCCA TGGGAAAACT GCAGGAGAAA CACCAGTTAT CCAGCATCAA AGAAGAGACA 
CCCACCTGTG CCGTCTCCAT CCAGTCCCGG GCAAGGCTGG TCGCTGGCTT CAGCAGTGGC 
TCCATTGCCC TGGTCTCCGC TGGAGAAGAC AGGCTGCTGG AGAAGCTCCC AGAAGCTGTG 
GGGTTCCTGG TGGTTTCTGA AGACGATTCC CTTCTTGTGG CAGGCTTTGG AAGATTTGTG 
CGGATTTTCT TGGCTGACTC GCAAGGCTTC CACCGGTTCA TGGCTTCGGA TCTGGAACAC 
GAGGACATGG TGGAGACAGC AGTCTTGGGT CCTGAGAACA ATCTGATCAT CACAGGGTCC 
CGGGATGCAC TTATCCAGGT GTGGAGTTTG TCAGAACAGG GGACCCTGCT GAATGTCCTT 
GAGGGCGTGG GCGCCCCTGT GAGTCTGCTG GTCCGGGGTG GGACTCTGGT GGTGTCTGCA 
TCCCGGAAGT CTTCATCTTT CAAGGTCTGG GATCTCAAGT CCACTAAGAA GCTGCAGTCT 
CCGACTCCAT TTCTGGACCG CACCGGCCTC GCTGCAGTGT CCCACCATGG CAGCTTTGTC 
TACTTCCCTA AGGTGAGGAG CAAGATTGCC TGGACACGTC CAATGAGGTC CGCTGTCTGG 
AGGTGGCCGA GCAGGCCAAG CTCCTCTTCA CAGGCCTGGT TTCTGGGATC GTGCTTGTGT 
TCCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531156.1
CDS	1111..4299
Translation	MVDRLVDGCLDTNAQSLLSGLKGRILDAQPGALKSHHLSWSRDLVNPKNKAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPGIGKTSLMCKLAQQVPELLGHKTVVVLRLLGTSKLSLDARSLLRSLSFQVCLAYGLPLPPAQVLEAHSRVGHFFHTLLHTVSQRNFESLVLLLDSVDDLDSICHSPRVSWLPLKCPPRVHLILSTCSGQQVLHNLQQTLKDPSTYWEVKALSGSQGQEFIQLLLAAEKRMLSPGQRDVLWASLPECGHPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCIRLEETHGALLVAHVLGYIVSSRYGLSEAELKDVLSLDDEVLQAVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLVRRPVDGCMLLAIAHRQLSQVIQVRYLSGPERAKRHGVLAEFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSSTVANLRKLTELPFHLLHAGRLEELKQEVLGNMSWISCRGISGGIEVLLDDFDMCAPHMNSPEVDLVREAIQLCGPAVELRGLEKSVLYTELLARLLFFAASHPAMIGQLCQQAQSWFRACPYPMLVPLAGFLQPPGGPLRATLTGCHKGITAIAWSLEEKLLVVGTQDGAMVVWDVEEQQVVHVLMGHTAEVKCVRVFAQGTLAISASKDHTLRLWSLLSGQEKVTILDGGSQNPTEPQSWDLHVDERNNVVYSTSGARINMWNLETSKLVFCITGDVSDPWVCVALLAAQGLLLALSKGGQVSLWSSAMGKLQEKHQLSSIKEETPTCAVSIQSRARLVAGFSSGSIALVSAGEDRLLEKLPEAVGFLVVSEDDSLLVAGFGRFVRIFLADSQGFHRFMASDLEHEDMVETAVLGPENNLIITGSRDALIQVWSLSEQGTLLNVLEGVGAPVSLLVRGGTLVVSASRKSSSFKVWDLKSTKKLQSPTPFLDRTGLAAVSHHGSFVYFPKVRSKIAWTRPMRSAVWRWPSRPSSSSQAWFLGSCLCSP

Target ORF information:

RefSeq Version	XM_006531093.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531093.2

ORF Insert Sequence:

ATGGTGGATC GGCTGGTAGA TGGCTGCCTG GATACCAATG CCCAGAGTCT TCTCAGTGGT 
CTTAAAGGGC GCATACTAGA TGCTCAGCCT GGAGCCCTAA AGTCCCACCA CCTGTCCTGG 
AGCCGAGACC TGGTCAACCC CAAGAACAAG GCTCACGCTC GTTACCTAAA GCAGTTGAGC 
GAGCAGTTCG TGGCCAGGAC CAACCATCAG GTCCTGGAGC AGCTTCGAGA GCTGGAGTTG 
GCCAGACAGG AGCTGGGTTG GCTCTACCAA GAGATCCGCC ACCACCTCTG GCAGAGCACA 
GAGTCTACCA AGGTCTTCTG CGGCCACCAG GAGCTCCTGG CCCAGCTCAG GCAGCAGCTC 
AGACAGGATG AGAGCCGGAC ACATACTCCC TTGGTTCTCT TTGGACCACC AGGCATCGGG 
AAGACCTCTC TCATGTGCAA GCTAGCCCAG CAGGTACCAG AGCTACTTGG CCACAAGACA 
GTGGTTGTCC TGAGGCTGCT AGGGACATCA AAGCTGAGCC TGGATGCCCG CAGCCTCCTC 
AGGAGCCTGT CCTTCCAAGT GTGCTTGGCA TATGGGCTGC CACTGCCCCC AGCCCAGGTC 
CTGGAGGCCC ACAGCAGGGT GGGCCATTTC TTCCATACTC TCCTCCACAC CGTGTCACAG 
AGGAACTTTG AGTCCCTGGT GTTGCTCCTG GACTCCGTGG ATGACCTGGA CTCCATATGC 
CATTCTCCGA GGGTCTCTTG GCTGCCCCTC AAGTGTCCTC CAAGGGTGCA CCTCATCCTC 
TCTACATGCT CAGGCCAGCA GGTTTTACAT AACCTGCAAC AGACCCTCAA GGACCCCAGC 
ACCTACTGGG AGGTGAAGGC CCTGTCTGGA AGTCAAGGCC AGGAGTTCAT ACAGCTGCTG 
CTGGCTGCAG AGAAGAGGAT GCTGAGCCCG GGTCAGAGAG ATGTCCTCTG GGCCAGCCTC 
CCAGAGTGTG GCCACCCAGG GAGGCTAAGG CTGGCCTTTG AGGAGGCCCG GAAGTGGGCC 
TCCTTCACTG TGCCAGTGCC CTTGGCCACT ACAGCAGAGG AAGCCACACA CCAGCTCTGT 
ATACGCCTGG AGGAGACACA TGGGGCACTG CTGGTGGCTC ATGTACTGGG CTATATTGTG 
TCTTCCAGGT ACGGTCTCTC AGAAGCAGAG CTGAAGGATG TGTTGTCTTT GGATGATGAA 
GTCTTGCAGG CTGTGTACCG GGACTGGACG CCACCTAGCA AAGAGCTGCT GCGCTTCCCG 
CCCCTGCTGT GGGTGCGGCT CCGCCGGGAC CTGGGACACT GCCTAGTCAG GAGGCCCGTG 
GATGGCTGCA TGCTACTGGC CATTGCACAC AGACAGCTGT CCCAGGTAAT CCAAGTTCGA 
TATCTGTCTG GGCCTGAGAG AGCCAAGAGG CATGGCGTGC TAGCTGAATT CTTCTCTGGA 
GCCTGGAGCC AGGGGATCAA GAAACTTATC ACCCTGCCGC TTGTAGGGAA GCCCCTGAAC 
CTGGACCGCA AGGTGGCCCC ACAGCCACTG TGGTTCTCAA GCACAGTGGC CAACCTGAGG 
AAGCTGACCG AACTGCCCTT CCATCTGCTG CATGCAGGCC GCTTGGAGGA GTTGAAGCAG 
GAGGTTCTGG GGAACATGAG TTGGATCTCA TGTCGGGGCA TCTCTGGAGG CATTGAGGTC 
CTGCTGGATG ATTTTGACAT GTGTGCCCCT CACATGAACT CTCCTGAGGT TGATCTGGTT 
AGAGAAGCCA TCCAGCTGTG TGGCCCTGCT GTGGAGCTCC GGGGCCTGGA GAAGAGCGTC 
CTATACACCG AGCTCCTGGC CAGACTCCTT TTCTTTGCTG CATCACATCC AGCAATGATC 
GGACAACTGT GCCAACAAGC CCAGAGCTGG TTCCGTGCCT GCCCCTACCC AATGCTGGTG 
CCCCTGGCAG GATTCCTGCA GCCACCTGGT GGACCTCTTC GAGCCACTCT TACTGGGTGT 
CACAAAGGCA TCACCGCCAT AGCATGGAGC CTGGAGGAGA AACTCCTGGT GGTGGGCACC 
CAGGATGGTG CCATGGTCGT GTGGGACGTG GAGGAACAAC AGGTGGTACA CGTTCTAATG 
GGACACACAG CTGAAGTGAA GTGTGTGCGA GTGTTTGCCC AAGGGACTCT TGCCATCTCT 
GCATCAAAGG ACCATACCCT GCGTCTGTGG AGTCTGCTTT CCGGCCAGGA GAAAGTCACC 
ATTTTGGATG GAGGGTCACA AAATCCCACA GAGCCCCAGA GTTGGGACCT TCATGTGGAT 
GAGAGAAACA ATGTTGTATA CTCGACATCT GGTGCAAGGA TCAACATGTG GAATCTGGAA 
ACTTCGAAGC TGGTTTTCTG TATCACAGGA GATGTGTCTG ATCCCTGGGT CTGTGTGGCT 
TTGTTGGCTG CCCAGGGTTT GCTGCTTGCC CTGTCCAAGG GTGGCCAGGT CAGTCTGTGG 
AGCTCAGCCA TGGGAAAACT GCAGGAGAAA CACCAGTTAT CCAGCATCAA AGAAGAGACA 
CCCACCTGTG CCGTCTCCAT CCAGTCCCGG GCAAGGCTGG TCGCTGGCTT CAGCAGTGGC 
TCCATTGCCC TGGTCTCCGC TGGAGAAGAC AGGCTGCTGG AGAAGCTCCC AGAAGCTGTG 
GGGTTCCTGG TGGTTTCTGA AGACGATTCC CTTCTTGTGG CAGGCTTTGG AAGATTTGTG 
CGGATTTTCT TGGCTGACTC GCAAGGCTTC CACCGGTTCA TGGCTTCGGA TCTGGAACAC 
GAGGACATGG TGGAGACAGC AGTCTTGGGT CCTGAGAACA ATCTGATCAT CACAGGGTCC 
CGGGATGCAC TTATCCAGGT GTGGAGTTTG TCAGAACAGG GGACCCTGCT GAATGTCCTT 
GAGGGCGTGG GCGCCCCTGT GAGTCTGCTG GTCCGGGGTG GGACTCTGGT GGTGTCTGCA 
TCCCGGAAGT CTTCATCTTT CAAGGTCTGG GATCTCAAGT CCACTAAGAA GCTGCAGTCT 
CCGACTCCAT TTCTGGACCG CACCGGCCTC GCTGCAGTGT CCCACCATGG CAGCTTTGTC 
TACTTCCCTA AGGTGAGGAG CAAGATTGCC TGGACACGTC CAATGAGGTC CGCTGTCTGG 
AGGTGGCCGA GCAGGCCAAG CTCCTCTTCA CAGGCCTGGT TTCTGGGATC GTGCTTGTGT 
TCCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nwd1 ( XM_006531087.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531150.1 Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X2, mRNA.

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CloneID	OMu41531
Clone ID Related Accession (Same CDS sequence)	XM_006531087.4 , XM_006531087.3
Accession Version	XM_006531087.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006531087.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAG AGCGAGAAGC CCTGCAGTGC ACAGCCTACC CTGAGGTTCA GAGCTTCTGC 
CAAAGGCACG GCTTGGCGTT TGAGGTGGTT GATCTGAGGT GGGGTATTCC GAATACGCAG 
GCCACTGACT ACCTGACCAC GGAACTCTGC TTAGAAGAAC TGGAGCGCTG TCAGAAAACA 
TCCATAGGAC CAGCTTTTGT GGCCCTCCTG GGTGACCAGT ACGGCCCCTG CCCGGTTCCC 
CGGAGGATTG AGGAAAAGGA GTGGGAGGCA CTAAGAGCTC AGCTGACCTC CAGGCCTCGA 
GATCTGGAGC TGGTGACCAG ACATTTCCAG AGAGACGACA ACACCATTCC TCCAACCTAC 
GTCTTGCAGC CTTCAGGCTC ACTGGTGGTC CCAGGGCCTG AGGAGGCCAC CTTAACCTCT 
GTCCTGAGAG GTGGAGCCCA GGAGGCCTGG AGGCTAGGCC TCATCAGCCA GGAGCAATGG 
ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG TACGGTCTCT CAGAAGCAGA GCTGAAGGAT 
GTGTTGTCTT TGGATGATGA AGTCTTGCAG GCTGTGTACC GGGACTGGAC GCCACCTAGC 
AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TCCGCCGGGA CCTGGGACAC 
TGCCTAGTCA GGAGGCCCGT GGATGGCTGC ATGCTACTGG CCATTGCACA CAGACAGCTG 
TCCCAGGTAA TCCAAGTTCG ATATCTGTCT GGGCCTGAGA GAGCCAAGAG GCATGGCGTG 
CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC CAGGGGATCA AGAAACTTAT CACCCTGCCG 
CTTGTAGGGA AGCCCCTGAA CCTGGACCGC AAGGTGGCCC CACAGCCACT GTGGTTCTCA 
AGCACAGTGG CCAACCTGAG GAAGCTGACC GAACTGCCCT TCCATCTGCT GCATGCAGGC 
CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG GGGAACATGA GTTGGATCTC ATGTCGGGGC 
ATCTCTGGAG GCATTGAGGT CCTGCTGGAT GATTTTGACA TGTGTGCCCC TCACATGAAC 
TCTCCTGAGG TTGATCTGGT TAGAGAAGCC ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC 
CGGGGCCTGG AGAAGAGCGT CCTATACACC GAGCTCCTGG CCAGACTCCT TTTCTTTGCT 
GCATCACATC CAGCAATGAT CGGACAACTG TGCCAACAAG CCCAGAGCTG GTTCCGTGCC 
TGCCCCTACC CAATGCTGGT GCCCCTGGCA GGATTCCTGC AGCCACCTGG TGGACCTCTT 
CGAGCCACTC TTACTGGGTG TCACAAAGGC ATCACCGCCA TAGCATGGAG CCTGGAGGAG 
AAACTCCTGG TGGTGGGCAC CCAGGATGGT GCCATGGTCG TGTGGGACGT GGAGGAACAA 
CAGGTGGTAC ACGTTCTAAT GGGACACACA GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC 
CAAGGGACTC TTGCCATCTC TGCATCAAAG GACCATACCC TGCGTCTGTG GAGTCTGCTT 
TCCGGCCAGG AGAAAGTCAC CATTTTGGAT GGAGGGTCAC AAAATCCCAC AGAGCCCCAG 
AGTTGGGACC TTCATGTGGA TGAGAGAAAC AATGTTGTAT ACTCGACATC TGGTGCAAGG 
ATCAACATGT GGAATCTGGA AACTTCGAAG CTGGTTTTCT GTATCACAGG AGATGTGTCT 
GATCCCTGGG TCTGTGTGGC TTTGTTGGCT GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG 
GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC ATGGGAAAAC TGCAGGAGAA ACACCAGTTA 
TCCAGCATCA AAGAAGAGAC ACCCACCTGT GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG 
GTCGCTGGCT TCAGCAGTGG CTCCATTGCC CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG 
GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG GTGGTTTCTG AAGACGATTC CCTTCTTGTG 
GCAGGCTTTG GAAGATTTGT GCGGATTTTC TTGGCTGACT CGCAAGGCTT CCACCGGTTC 
ATGGCTTCGG ATCTGGAACA CGAGGACATG GTGGAGACAG CAGTCTTGGG TCCTGAGAAC 
AATCTGATCA TCACAGGGTC CCGGGATGCA CTTATCCAGG TGTGGAGTTT GTCAGAACAG 
GGGACCCTGC TGAATGTCCT TGAGGGCGTG GGCGCCCCTG TGAGTCTGCT GGTCCGGGGT 
GGGACTCTGG TGGTGTCTGC ATCCCGGAAG TCTTCATCTT TCAAGGTCTG GGATCTCAAG 
TCCACTAAGA AGCTGCAGTC TCCGACTCCA TTTCTGGACC GCACCGGCCT CGCTGCAGTG 
TCCCACCATG GCAGCTTTGT CTACTTCCCT AAGGTTGGGG ACAAAAACAA AGTCACAATT 
TGGGACTTGG CAGAAGGTGA GGAGCAAGAT TGCCTGGACA CGTCCAATGA GGTCCGCTGT 
CTGGAGGTGG CCGAGCAGGC CAAGCTCCTC TTCACAGGCC TGGTTTCTGG GATCGTGCTT 
GTGTTCCCCC TGAATTCCAG GCAAGATGTA CTGTGCATCC CCCCACCTGA GGCACGCAAA 
GCTGTCAACT GCATGTCCCT GAGCAAGAGT GAGAATCGTC TGGCCATTGC CTATGACAAC 
ATTGTCCTGG TGCTTGACAT CAGCCCTGGG GACCCATGTC CGGCCATTGA AGGCCCCACC 
TACACCTTTT ACACCCAGCT GCCTGAGACC ATAGTGAGTG TGGCTGTCCT GGCTGACTAT 
CGTGTGGTCT ACGGCATGAG CGATGGCAGC CTCTTCCTGT ACGACTGTGC ATGCTCTAAA 
GTGTTCCCTC TGGAGGCCCA TGGGAGCAGG GTGAGCTGTG TAGAGGTCAG CCACAGTGAG 
CAGCTAGCAG TGAGCGGGGC TGAAGATGCA CTGCTATGCC TCTGGGACCT GCAGGCTTGC 
AGGGGGATGT TCGAGATGAG CTATGAGAAC TCCTGCTGCA GAGGGGTGCG GTGTGCCTGC 
TTCTCCAGGG ATGACAAGCA TGTATTTGCA GGCATGGAGG ACCGCTCTGT CACTGCCTGG 
AGCACGGTGG ATGGCACTCT TTTGGCTGTC CAGTTTGTTC ATGCTGTGAT CAACCGAATC 
ATCCCTACCT CCAATGGCTT CATGGCCCCC ACAAGCCACG GCTATCTCAT CCGTGAGCGC 
TTCCAGTGCC CGTCAGTCAG AGCCTCCCAG CAAGACCCTC TCAAGAACTT CAAGAAGGCA 
GTGTGGCTGG TAAAGACCAG GCAGAGGGAA GAGCTGGCTG CAGCAGAAGC CTCCCAGGAT 
GCAGAACCAG TGGCCGTAGA GGGAAAGGAA TCAAAGTCAA ATAAACGCTC ACAGGTCTGC 
CTCATACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531150.1
CDS	785..5476
Translation	MDTEREALQCTAYPEVQSFCQRHGLAFEVVDLRWGIPNTQATDYLTTELCLEELERCQKTSIGPAFVALLGDQYGPCPVPRRIEEKEWEALRAQLTSRPRDLELVTRHFQRDDNTIPPTYVLQPSGSLVVPGPEEATLTSVLRGGAQEAWRLGLISQEQWMCYHRSVIEWEIELGLLSSARGDQGATVFLRDVQDLNKHILDDCSLKMVDRLVDGCLDTNAQSLLSGLKGRILDAQPGALKSHHLSWSRDLVNPKNKAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPGIGKTSLMCKLAQQVPELLGHKTVVVLRLLGTSKLSLDARSLLRSLSFQVCLAYGLPLPPAQVLEAHSRVGHFFHTLLHTVSQRNFESLVLLLDSVDDLDSICHSPRVSWLPLKCPPRVHLILSTCSGQQVLHNLQQTLKDPSTYWEVKALSGSQGQEFIQLLLAAEKRMLSPGQRDVLWASLPECGHPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCIRLEETHGALLVAHVLGYIVSSRYGLSEAELKDVLSLDDEVLQAVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLVRRPVDGCMLLAIAHRQLSQVIQVRYLSGPERAKRHGVLAEFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSSTVANLRKLTELPFHLLHAGRLEELKQEVLGNMSWISCRGISGGIEVLLDDFDMCAPHMNSPEVDLVREAIQLCGPAVELRGLEKSVLYTELLARLLFFAASHPAMIGQLCQQAQSWFRACPYPMLVPLAGFLQPPGGPLRATLTGCHKGITAIAWSLEEKLLVVGTQDGAMVVWDVEEQQVVHVLMGHTAEVKCVRVFAQGTLAISASKDHTLRLWSLLSGQEKVTILDGGSQNPTEPQSWDLHVDERNNVVYSTSGARINMWNLETSKLVFCITGDVSDPWVCVALLAAQGLLLALSKGGQVSLWSSAMGKLQEKHQLSSIKEETPTCAVSIQSRARLVAGFSSGSIALVSAGEDRLLEKLPEAVGFLVVSEDDSLLVAGFGRFVRIFLADSQGFHRFMASDLEHEDMVETAVLGPENNLIITGSRDALIQVWSLSEQGTLLNVLEGVGAPVSLLVRGGTLVVSASRKSSSFKVWDLKSTKKLQSPTPFLDRTGLAAVSHHGSFVYFPKVGDKNKVTIWDLAEGEEQDCLDTSNEVRCLEVAEQAKLLFTGLVSGIVLVFPLNSRQDVLCIPPPEARKAVNCMSLSKSENRLAIAYDNIVLVLDISPGDPCPAIEGPTYTFYTQLPETIVSVAVLADYRVVYGMSDGSLFLYDCACSKVFPLEAHGSRVSCVEVSHSEQLAVSGAEDALLCLWDLQACRGMFEMSYENSCCRGVRCACFSRDDKHVFAGMEDRSVTAWSTVDGTLLAVQFVHAVINRIIPTSNGFMAPTSHGYLIRERFQCPSVRASQQDPLKNFKKAVWLVKTRQREELAAAEASQDAEPVAVEGKESKSNKRSQVCLIL

Target ORF information:

RefSeq Version	XM_006531087.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531087.4

ORF Insert Sequence:

ATGGACACAG AGCGAGAAGC CCTGCAGTGC ACAGCCTACC CTGAGGTTCA GAGCTTCTGC 
CAAAGGCACG GCTTGGCGTT TGAGGTGGTT GATCTGAGGT GGGGTATTCC GAATACGCAG 
GCCACTGACT ACCTGACCAC GGAACTCTGC TTAGAAGAAC TGGAGCGCTG TCAGAAAACA 
TCCATAGGAC CAGCTTTTGT GGCCCTCCTG GGTGACCAGT ACGGCCCCTG CCCGGTTCCC 
CGGAGGATTG AGGAAAAGGA GTGGGAGGCA CTAAGAGCTC AGCTGACCTC CAGGCCTCGA 
GATCTGGAGC TGGTGACCAG ACATTTCCAG AGAGACGACA ACACCATTCC TCCAACCTAC 
GTCTTGCAGC CTTCAGGCTC ACTGGTGGTC CCAGGGCCTG AGGAGGCCAC CTTAACCTCT 
GTCCTGAGAG GTGGAGCCCA GGAGGCCTGG AGGCTAGGCC TCATCAGCCA GGAGCAATGG 
ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG TACGGTCTCT CAGAAGCAGA GCTGAAGGAT 
GTGTTGTCTT TGGATGATGA AGTCTTGCAG GCTGTGTACC GGGACTGGAC GCCACCTAGC 
AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TCCGCCGGGA CCTGGGACAC 
TGCCTAGTCA GGAGGCCCGT GGATGGCTGC ATGCTACTGG CCATTGCACA CAGACAGCTG 
TCCCAGGTAA TCCAAGTTCG ATATCTGTCT GGGCCTGAGA GAGCCAAGAG GCATGGCGTG 
CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC CAGGGGATCA AGAAACTTAT CACCCTGCCG 
CTTGTAGGGA AGCCCCTGAA CCTGGACCGC AAGGTGGCCC CACAGCCACT GTGGTTCTCA 
AGCACAGTGG CCAACCTGAG GAAGCTGACC GAACTGCCCT TCCATCTGCT GCATGCAGGC 
CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG GGGAACATGA GTTGGATCTC ATGTCGGGGC 
ATCTCTGGAG GCATTGAGGT CCTGCTGGAT GATTTTGACA TGTGTGCCCC TCACATGAAC 
TCTCCTGAGG TTGATCTGGT TAGAGAAGCC ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC 
CGGGGCCTGG AGAAGAGCGT CCTATACACC GAGCTCCTGG CCAGACTCCT TTTCTTTGCT 
GCATCACATC CAGCAATGAT CGGACAACTG TGCCAACAAG CCCAGAGCTG GTTCCGTGCC 
TGCCCCTACC CAATGCTGGT GCCCCTGGCA GGATTCCTGC AGCCACCTGG TGGACCTCTT 
CGAGCCACTC TTACTGGGTG TCACAAAGGC ATCACCGCCA TAGCATGGAG CCTGGAGGAG 
AAACTCCTGG TGGTGGGCAC CCAGGATGGT GCCATGGTCG TGTGGGACGT GGAGGAACAA 
CAGGTGGTAC ACGTTCTAAT GGGACACACA GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC 
CAAGGGACTC TTGCCATCTC TGCATCAAAG GACCATACCC TGCGTCTGTG GAGTCTGCTT 
TCCGGCCAGG AGAAAGTCAC CATTTTGGAT GGAGGGTCAC AAAATCCCAC AGAGCCCCAG 
AGTTGGGACC TTCATGTGGA TGAGAGAAAC AATGTTGTAT ACTCGACATC TGGTGCAAGG 
ATCAACATGT GGAATCTGGA AACTTCGAAG CTGGTTTTCT GTATCACAGG AGATGTGTCT 
GATCCCTGGG TCTGTGTGGC TTTGTTGGCT GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG 
GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC ATGGGAAAAC TGCAGGAGAA ACACCAGTTA 
TCCAGCATCA AAGAAGAGAC ACCCACCTGT GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG 
GTCGCTGGCT TCAGCAGTGG CTCCATTGCC CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG 
GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG GTGGTTTCTG AAGACGATTC CCTTCTTGTG 
GCAGGCTTTG GAAGATTTGT GCGGATTTTC TTGGCTGACT CGCAAGGCTT CCACCGGTTC 
ATGGCTTCGG ATCTGGAACA CGAGGACATG GTGGAGACAG CAGTCTTGGG TCCTGAGAAC 
AATCTGATCA TCACAGGGTC CCGGGATGCA CTTATCCAGG TGTGGAGTTT GTCAGAACAG 
GGGACCCTGC TGAATGTCCT TGAGGGCGTG GGCGCCCCTG TGAGTCTGCT GGTCCGGGGT 
GGGACTCTGG TGGTGTCTGC ATCCCGGAAG TCTTCATCTT TCAAGGTCTG GGATCTCAAG 
TCCACTAAGA AGCTGCAGTC TCCGACTCCA TTTCTGGACC GCACCGGCCT CGCTGCAGTG 
TCCCACCATG GCAGCTTTGT CTACTTCCCT AAGGTTGGGG ACAAAAACAA AGTCACAATT 
TGGGACTTGG CAGAAGGTGA GGAGCAAGAT TGCCTGGACA CGTCCAATGA GGTCCGCTGT 
CTGGAGGTGG CCGAGCAGGC CAAGCTCCTC TTCACAGGCC TGGTTTCTGG GATCGTGCTT 
GTGTTCCCCC TGAATTCCAG GCAAGATGTA CTGTGCATCC CCCCACCTGA GGCACGCAAA 
GCTGTCAACT GCATGTCCCT GAGCAAGAGT GAGAATCGTC TGGCCATTGC CTATGACAAC 
ATTGTCCTGG TGCTTGACAT CAGCCCTGGG GACCCATGTC CGGCCATTGA AGGCCCCACC 
TACACCTTTT ACACCCAGCT GCCTGAGACC ATAGTGAGTG TGGCTGTCCT GGCTGACTAT 
CGTGTGGTCT ACGGCATGAG CGATGGCAGC CTCTTCCTGT ACGACTGTGC ATGCTCTAAA 
GTGTTCCCTC TGGAGGCCCA TGGGAGCAGG GTGAGCTGTG TAGAGGTCAG CCACAGTGAG 
CAGCTAGCAG TGAGCGGGGC TGAAGATGCA CTGCTATGCC TCTGGGACCT GCAGGCTTGC 
AGGGGGATGT TCGAGATGAG CTATGAGAAC TCCTGCTGCA GAGGGGTGCG GTGTGCCTGC 
TTCTCCAGGG ATGACAAGCA TGTATTTGCA GGCATGGAGG ACCGCTCTGT CACTGCCTGG 
AGCACGGTGG ATGGCACTCT TTTGGCTGTC CAGTTTGTTC ATGCTGTGAT CAACCGAATC 
ATCCCTACCT CCAATGGCTT CATGGCCCCC ACAAGCCACG GCTATCTCAT CCGTGAGCGC 
TTCCAGTGCC CGTCAGTCAG AGCCTCCCAG CAAGACCCTC TCAAGAACTT CAAGAAGGCA 
GTGTGGCTGG TAAAGACCAG GCAGAGGGAA GAGCTGGCTG CAGCAGAAGC CTCCCAGGAT 
GCAGAACCAG TGGCCGTAGA GGGAAAGGAA TCAAAGTCAA ATAAACGCTC ACAGGTCTGC 
CTCATACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nwd1 ( NM_176940.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_795914.3 Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), mRNA.

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CloneID	OMu05763
Clone ID Related Accession (Same CDS sequence)	NM_176940.5
Accession Version	NM_176940.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Mus musculus(house mouse)
Product	NACHT domain- and WD repeat-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK087622.1 and BC082552.1. On Apr 29, 2010 this sequence version replaced NM_176940.4. ##Evidence-Data-START## Transcript exon combination :: BC082552.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGACACAG AGCGAGAAGC CCTGCAGTGC ACAGCCTACC CTGAGGTTCA GAGCTTCTGC 
CAAAGGCACG GCTTGGCGTT TGAGGTGGTT GATCTGAGGT GGGGTATTCC GAATACGCAG 
GCCACTGACT ACCTGACCAC GGAACTCTGC TTAGAAGAAC TGGAGCGCTG TCAGAAAACA 
TCCATAGGAC CAGCTTTTGT GGCCCTCCTG GGTGACCAGT ACGGCCCCTG CCCGGTTCCC 
CGGAGGATTG AGGAAAAGGA GTGGGAGGCA CTAAGAGCTC AGCTGACCTC CAGGCCTCGA 
GATCTGGAGC TGGTGACCAG ACATTTCCAG AGAGACGACA ACACCATTCC TCCAACCTAC 
GTCTTGCAGC CTTCAGGCTC ACTGGTGGTC CCAGGGCCTG AGGAGGCCAC CTTAACCTCT 
GTCCTGAGAG GTGGAGCCCA GGAGGCCTGG AGGCTAGGCC TCATCAGCCA GGAGCAATGG 
ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG TACGGTCTCT CAGAAGCAGA GCTGAAGGAT 
GTGTTGTCTT TGGATGATGA AGTCTTGCAG GCTGTGTACC GGGACTGGAC GCCACCTAGC 
AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TCCGCCGGGA CCTGGGACAC 
TGCCTAGTCA GGAGGCCCGT GGATGGCTGC ATGCTACTGG CCATTGCACA CAGACAGCTG 
TCCCAGGTAA TCCAAGTTCG ATATCTGTCT GGGCCTGAGA GAGCCAAGAG GCATGGCGTG 
CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC CAGGGGATCA AGAAACTTAT CACCCTGCCG 
CTTGTAGGGA AGCCCCTGAA CCTGGACCGC AAGGTGGCCC CACAGCCACT GTGGTTCTCA 
AGCACAGTGG CCAACCTGAG GAAGCTGACC GAACTGCCCT TCCATCTGCT GCATGCAGGC 
CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG GGGAACATGA GTTGGATCTC ATGTCGGGGC 
ATCTCTGGAG GCATTGAGGT CCTGCTGGAT GATTTTGACA TGTGTGCCCC TCACATGAAC 
TCTCCTGAGG TTGATCTGGT TAGAGAAGCC ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC 
CGGGGCCTGG AGAAGAGCGT CCTATACACC GAGCTCCTGG CCAGACTCCT TTTCTTTGCT 
GCATCACATC CAGCAATGAT CGGACAACTG TGCCAACAAG CCCAGAGCTG GTTCCGTGCC 
TGCCCCTACC CAATGCTGGT GCCCCTGGCA GGATTCCTGC AGCCACCTGG TGGACCTCTT 
CGAGCCACTC TTACTGGGTG TCACAAAGGC ATCACCGCCA TAGCATGGAG CCTGGAGGAG 
AAACTCCTGG TGGTGGGCAC CCAGGATGGT GCCATGGTCG TGTGGGACGT GGAGGAACAA 
CAGGTGGTAC ACGTTCTAAT GGGACACACA GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC 
CAAGGGACTC TTGCCATCTC TGCATCAAAG GACCATACCC TGCGTCTGTG GAGTCTGCTT 
TCCGGCCAGG AGAAAGTCAC CATTTTGGAT GGAGGGTCAC AAAATCCCAC AGAGCCCCAG 
AGTTGGGACC TTCATGTGGA TGAGAGAAAC AATGTTGTAT ACTCGACATC TGGTGCAAGG 
ATCAACATGT GGAATCTGGA AACTTCGAAG CTGGTTTTCT GTATCACAGG AGATGTGTCT 
GATCCCTGGG TCTGTGTGGC TTTGTTGGCT GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG 
GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC ATGGGAAAAC TGCAGGAGAA ACACCAGTTA 
TCCAGCATCA AAGAAGAGAC ACCCACCTGT GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG 
GTCGCTGGCT TCAGCAGTGG CTCCATTGCC CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG 
GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG GTGGTTTCTG AAGACGATTC CCTTCTTGTG 
GCAGGCTTTG GAAGATTTGT GCGGATTTTC TTGGCTGACT CGCAAGGCTT CCACCGGTTC 
ATGGCTTCGG ATCTGGAACA CGAGGACATG GTGGAGACAG CAGTCTTGGG TCCTGAGAAC 
AATCTGATCA TCACAGGGTC CCGGGATGCA CTTATCCAGG TCTGGGATCT CAAGTCCACT 
AAGAAGCTGC AGTCTCCGAC TCCATTTCTG GACCGCACCG GCCTCGCTGC AGTGTCCCAC 
CATGGCAGCT TTGTCTACTT CCCTAAGGTT GGGGACAAAA ACAAAGTCAC AATTTGGGAC 
TTGGCAGAAG GTGAGGAGCA AGATTGCCTG GACACGTCCA ATGAGGTCCG CTGTCTGGAG 
GTGGCCGAGC AGGCCAAGCT CCTCTTCACA GGCCTGGTTT CTGGGATCGT GCTTGTGTTC 
CCCCTGAATT CCAGGCAAGA TGTACTGTGC ATCCCCCCAC CTGAGGCACG CAAAGCTGTC 
AACTGCATGT CCCTGAGCAA GAGTGAGAAT CGTCTGGCCA TTGCCTATGA CAACATTGTC 
CTGGTGCTTG ACATCAGCCC TGGGGACCCA TGTCCGGCCA TTGAAGGCCC CACCTACACC 
TTTTACACCC AGCTGCCTGA GACCATAGTG AGTGTGGCTG TCCTGGCTGA CTATCGTGTG 
GTCTACGGCA TGAGCGATGG CAGCCTCTTC CTGTACGACT GTGCATGCTC TAAAGTGTTC 
CCTCTGGAGG CCCATGGGAG CAGGGTGAGC TGTGTAGAGG TCAGCCACAG TGAGCAGCTA 
GCAGTGAGCG GGGCTGAAGA TGCACTGCTA TGCCTCTGGG ACCTGCAGGC TTGCAGGGGG 
ATGTTCGAGA TGAGCTATGA GAACTCCTGC TGCAGAGGGG TGCGGTGTGC CTGCTTCTCC 
AGGGATGACA AGCATGTATT TGCAGGCATG GAGGACCGCT CTGTCACTGC CTGGAGCACG 
GTGGATGGCA CTCTTTTGGC TGTCCAGTTT GTTCATGCTG TGATCAACCG AATCATCCCT 
ACCTCCAATG GCTTCATGGC CCCCACAAGC CACGGCTATC TCATCCGTGA GCGCTTCCAG 
TGCCCGTCAG TCAGAGCCTC CCAGCAAGAC CCTCTCAAGA ACTTCAAGAA GGCAGTGTGG 
CTGGTAAAGA CCAGGCAGAG GGAAGAGCTG GCTGCAGCAG AAGCCTCCCA GGATGCAGAA 
CCAGTGGCCG TAGAGGGAAA GGAATCAAAG TCAAATAAAC GCTCACAGGT CTGCCTCATA 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_795914.3
CDS	302..4867
Translation	MDTEREALQCTAYPEVQSFCQRHGLAFEVVDLRWGIPNTQATDYLTTELCLEELERCQKTSIGPAFVALLGDQYGPCPVPRRIEEKEWEALRAQLTSRPRDLELVTRHFQRDDNTIPPTYVLQPSGSLVVPGPEEATLTSVLRGGAQEAWRLGLISQEQWMCYHRSVIEWEIELGLLSSARGDQGATVFLRDVQDLNKHILDDCSLKMVDRLVDGCLDTNAQSLLSGLKGRILDAQPGALKSHHLSWSRDLVNPKNKAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPGIGKTSLMCKLAQQVPELLGHKTVVVLRLLGTSKLSLDARSLLRSLSFQVCLAYGLPLPPAQVLEAHSRVGHFFHTLLHTVSQRNFESLVLLLDSVDDLDSICHSPRVSWLPLKCPPRVHLILSTCSGQQVLHNLQQTLKDPSTYWEVKALSGSQGQEFIQLLLAAEKRMLSPGQRDVLWASLPECGHPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCIRLEETHGALLVAHVLGYIVSSRYGLSEAELKDVLSLDDEVLQAVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLVRRPVDGCMLLAIAHRQLSQVIQVRYLSGPERAKRHGVLAEFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSSTVANLRKLTELPFHLLHAGRLEELKQEVLGNMSWISCRGISGGIEVLLDDFDMCAPHMNSPEVDLVREAIQLCGPAVELRGLEKSVLYTELLARLLFFAASHPAMIGQLCQQAQSWFRACPYPMLVPLAGFLQPPGGPLRATLTGCHKGITAIAWSLEEKLLVVGTQDGAMVVWDVEEQQVVHVLMGHTAEVKCVRVFAQGTLAISASKDHTLRLWSLLSGQEKVTILDGGSQNPTEPQSWDLHVDERNNVVYSTSGARINMWNLETSKLVFCITGDVSDPWVCVALLAAQGLLLALSKGGQVSLWSSAMGKLQEKHQLSSIKEETPTCAVSIQSRARLVAGFSSGSIALVSAGEDRLLEKLPEAVGFLVVSEDDSLLVAGFGRFVRIFLADSQGFHRFMASDLEHEDMVETAVLGPENNLIITGSRDALIQVWDLKSTKKLQSPTPFLDRTGLAAVSHHGSFVYFPKVGDKNKVTIWDLAEGEEQDCLDTSNEVRCLEVAEQAKLLFTGLVSGIVLVFPLNSRQDVLCIPPPEARKAVNCMSLSKSENRLAIAYDNIVLVLDISPGDPCPAIEGPTYTFYTQLPETIVSVAVLADYRVVYGMSDGSLFLYDCACSKVFPLEAHGSRVSCVEVSHSEQLAVSGAEDALLCLWDLQACRGMFEMSYENSCCRGVRCACFSRDDKHVFAGMEDRSVTAWSTVDGTLLAVQFVHAVINRIIPTSNGFMAPTSHGYLIRERFQCPSVRASQQDPLKNFKKAVWLVKTRQREELAAAEASQDAEPVAVEGKESKSNKRSQVCLIL

Target ORF information:

RefSeq Version	NM_176940.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176940.5

ORF Insert Sequence:

ATGGACACAG AGCGAGAAGC CCTGCAGTGC ACAGCCTACC CTGAGGTTCA GAGCTTCTGC 
CAAAGGCACG GCTTGGCGTT TGAGGTGGTT GATCTGAGGT GGGGTATTCC GAATACGCAG 
GCCACTGACT ACCTGACCAC GGAACTCTGC TTAGAAGAAC TGGAGCGCTG TCAGAAAACA 
TCCATAGGAC CAGCTTTTGT GGCCCTCCTG GGTGACCAGT ACGGCCCCTG CCCGGTTCCC 
CGGAGGATTG AGGAAAAGGA GTGGGAGGCA CTAAGAGCTC AGCTGACCTC CAGGCCTCGA 
GATCTGGAGC TGGTGACCAG ACATTTCCAG AGAGACGACA ACACCATTCC TCCAACCTAC 
GTCTTGCAGC CTTCAGGCTC ACTGGTGGTC CCAGGGCCTG AGGAGGCCAC CTTAACCTCT 
GTCCTGAGAG GTGGAGCCCA GGAGGCCTGG AGGCTAGGCC TCATCAGCCA GGAGCAATGG 
ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG TACGGTCTCT CAGAAGCAGA GCTGAAGGAT 
GTGTTGTCTT TGGATGATGA AGTCTTGCAG GCTGTGTACC GGGACTGGAC GCCACCTAGC 
AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TCCGCCGGGA CCTGGGACAC 
TGCCTAGTCA GGAGGCCCGT GGATGGCTGC ATGCTACTGG CCATTGCACA CAGACAGCTG 
TCCCAGGTAA TCCAAGTTCG ATATCTGTCT GGGCCTGAGA GAGCCAAGAG GCATGGCGTG 
CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC CAGGGGATCA AGAAACTTAT CACCCTGCCG 
CTTGTAGGGA AGCCCCTGAA CCTGGACCGC AAGGTGGCCC CACAGCCACT GTGGTTCTCA 
AGCACAGTGG CCAACCTGAG GAAGCTGACC GAACTGCCCT TCCATCTGCT GCATGCAGGC 
CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG GGGAACATGA GTTGGATCTC ATGTCGGGGC 
ATCTCTGGAG GCATTGAGGT CCTGCTGGAT GATTTTGACA TGTGTGCCCC TCACATGAAC 
TCTCCTGAGG TTGATCTGGT TAGAGAAGCC ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC 
CGGGGCCTGG AGAAGAGCGT CCTATACACC GAGCTCCTGG CCAGACTCCT TTTCTTTGCT 
GCATCACATC CAGCAATGAT CGGACAACTG TGCCAACAAG CCCAGAGCTG GTTCCGTGCC 
TGCCCCTACC CAATGCTGGT GCCCCTGGCA GGATTCCTGC AGCCACCTGG TGGACCTCTT 
CGAGCCACTC TTACTGGGTG TCACAAAGGC ATCACCGCCA TAGCATGGAG CCTGGAGGAG 
AAACTCCTGG TGGTGGGCAC CCAGGATGGT GCCATGGTCG TGTGGGACGT GGAGGAACAA 
CAGGTGGTAC ACGTTCTAAT GGGACACACA GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC 
CAAGGGACTC TTGCCATCTC TGCATCAAAG GACCATACCC TGCGTCTGTG GAGTCTGCTT 
TCCGGCCAGG AGAAAGTCAC CATTTTGGAT GGAGGGTCAC AAAATCCCAC AGAGCCCCAG 
AGTTGGGACC TTCATGTGGA TGAGAGAAAC AATGTTGTAT ACTCGACATC TGGTGCAAGG 
ATCAACATGT GGAATCTGGA AACTTCGAAG CTGGTTTTCT GTATCACAGG AGATGTGTCT 
GATCCCTGGG TCTGTGTGGC TTTGTTGGCT GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG 
GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC ATGGGAAAAC TGCAGGAGAA ACACCAGTTA 
TCCAGCATCA AAGAAGAGAC ACCCACCTGT GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG 
GTCGCTGGCT TCAGCAGTGG CTCCATTGCC CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG 
GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG GTGGTTTCTG AAGACGATTC CCTTCTTGTG 
GCAGGCTTTG GAAGATTTGT GCGGATTTTC TTGGCTGACT CGCAAGGCTT CCACCGGTTC 
ATGGCTTCGG ATCTGGAACA CGAGGACATG GTGGAGACAG CAGTCTTGGG TCCTGAGAAC 
AATCTGATCA TCACAGGGTC CCGGGATGCA CTTATCCAGG TCTGGGATCT CAAGTCCACT 
AAGAAGCTGC AGTCTCCGAC TCCATTTCTG GACCGCACCG GCCTCGCTGC AGTGTCCCAC 
CATGGCAGCT TTGTCTACTT CCCTAAGGTT GGGGACAAAA ACAAAGTCAC AATTTGGGAC 
TTGGCAGAAG GTGAGGAGCA AGATTGCCTG GACACGTCCA ATGAGGTCCG CTGTCTGGAG 
GTGGCCGAGC AGGCCAAGCT CCTCTTCACA GGCCTGGTTT CTGGGATCGT GCTTGTGTTC 
CCCCTGAATT CCAGGCAAGA TGTACTGTGC ATCCCCCCAC CTGAGGCACG CAAAGCTGTC 
AACTGCATGT CCCTGAGCAA GAGTGAGAAT CGTCTGGCCA TTGCCTATGA CAACATTGTC 
CTGGTGCTTG ACATCAGCCC TGGGGACCCA TGTCCGGCCA TTGAAGGCCC CACCTACACC 
TTTTACACCC AGCTGCCTGA GACCATAGTG AGTGTGGCTG TCCTGGCTGA CTATCGTGTG 
GTCTACGGCA TGAGCGATGG CAGCCTCTTC CTGTACGACT GTGCATGCTC TAAAGTGTTC 
CCTCTGGAGG CCCATGGGAG CAGGGTGAGC TGTGTAGAGG TCAGCCACAG TGAGCAGCTA 
GCAGTGAGCG GGGCTGAAGA TGCACTGCTA TGCCTCTGGG ACCTGCAGGC TTGCAGGGGG 
ATGTTCGAGA TGAGCTATGA GAACTCCTGC TGCAGAGGGG TGCGGTGTGC CTGCTTCTCC 
AGGGATGACA AGCATGTATT TGCAGGCATG GAGGACCGCT CTGTCACTGC CTGGAGCACG 
GTGGATGGCA CTCTTTTGGC TGTCCAGTTT GTTCATGCTG TGATCAACCG AATCATCCCT 
ACCTCCAATG GCTTCATGGC CCCCACAAGC CACGGCTATC TCATCCGTGA GCGCTTCCAG 
TGCCCGTCAG TCAGAGCCTC CCAGCAAGAC CCTCTCAAGA ACTTCAAGAA GGCAGTGTGG 
CTGGTAAAGA CCAGGCAGAG GGAAGAGCTG GCTGCAGCAG AAGCCTCCCA GGATGCAGAA 
CCAGTGGCCG TAGAGGGAAA GGAATCAAAG TCAAATAAAC GCTCACAGGT CTGCCTCATA 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nwd1 ( XM_006531086.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531149.1 Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu41530
Clone ID Related Accession (Same CDS sequence)	XM_006531086.2
Accession Version	XM_006531086.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006531086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAG AGCGAGAAGC CCTGCAGTGC ACAGCCTACC CTGAGGTTCA GAGCTTCTGC 
CAAAGGCACG GCTTGGCGTT TGAGGTGGTT GATCTGAGGT GGGGTATTCC GAATACGCAG 
GCCACTGACT ACCTGACCAC GGAACTCTGC TTAGAAGAAC TGGAGCGCTG TCAGAAAACA 
TCCATAGGAC CAGCTTTTGT GGCCCTCCTG GGTGACCAGT ACGGCCCCTG CCCGGTTCCC 
CGGAGGATTG AGGAAAAGGA GTGGGAGGCA CTAAGAGCTC AGCTGACCTC CAGGCCTCGA 
GATCTGGAGC TGGTGACCAG ACATTTCCAG AGAGACGACA ACACCATTCC TCCAACCTAC 
GTCTTGCAGC CTTCAGGCTC ACTGGTGGTC CCAGGGCCTG AGGAGGCCAC CTTAACCTCT 
GTCCTGAGAG GTGGAGCCCA GGAGGCCTGG AGGCTAGGCC TCATCAGCCA GGAGCAATGG 
ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG GATTTGGACA GAACCTGGAT TTTGTGGAGG 
TACGGTCTCT CAGAAGCAGA GCTGAAGGAT GTGTTGTCTT TGGATGATGA AGTCTTGCAG 
GCTGTGTACC GGGACTGGAC GCCACCTAGC AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG 
TGGGTGCGGC TCCGCCGGGA CCTGGGACAC TGCCTAGTCA GGAGGCCCGT GGATGGCTGC 
ATGCTACTGG CCATTGCACA CAGACAGCTG TCCCAGGTAA TCCAAGTTCG ATATCTGTCT 
GGGCCTGAGA GAGCCAAGAG GCATGGCGTG CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC 
CAGGGGATCA AGAAACTTAT CACCCTGCCG CTTGTAGGGA AGCCCCTGAA CCTGGACCGC 
AAGGTGGCCC CACAGCCACT GTGGTTCTCA AGCACAGTGG CCAACCTGAG GAAGCTGACC 
GAACTGCCCT TCCATCTGCT GCATGCAGGC CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG 
GGGAACATGA GTTGGATCTC ATGTCGGGGC ATCTCTGGAG GCATTGAGGT CCTGCTGGAT 
GATTTTGACA TGTGTGCCCC TCACATGAAC TCTCCTGAGG TTGATCTGGT TAGAGAAGCC 
ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC CGGGGCCTGG AGAAGAGCGT CCTATACACC 
GAGCTCCTGG CCAGACTCCT TTTCTTTGCT GCATCACATC CAGCAATGAT CGGACAACTG 
TGCCAACAAG CCCAGAGCTG GTTCCGTGCC TGCCCCTACC CAATGCTGGT GCCCCTGGCA 
GGATTCCTGC AGCCACCTGG TGGACCTCTT CGAGCCACTC TTACTGGGTG TCACAAAGGC 
ATCACCGCCA TAGCATGGAG CCTGGAGGAG AAACTCCTGG TGGTGGGCAC CCAGGATGGT 
GCCATGGTCG TGTGGGACGT GGAGGAACAA CAGGTGGTAC ACGTTCTAAT GGGACACACA 
GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC CAAGGGACTC TTGCCATCTC TGCATCAAAG 
GACCATACCC TGCGTCTGTG GAGTCTGCTT TCCGGCCAGG AGAAAGTCAC CATTTTGGAT 
GGAGGGTCAC AAAATCCCAC AGAGCCCCAG AGTTGGGACC TTCATGTGGA TGAGAGAAAC 
AATGTTGTAT ACTCGACATC TGGTGCAAGG ATCAACATGT GGAATCTGGA AACTTCGAAG 
CTGGTTTTCT GTATCACAGG AGATGTGTCT GATCCCTGGG TCTGTGTGGC TTTGTTGGCT 
GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC 
ATGGGAAAAC TGCAGGAGAA ACACCAGTTA TCCAGCATCA AAGAAGAGAC ACCCACCTGT 
GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG GTCGCTGGCT TCAGCAGTGG CTCCATTGCC 
CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG 
GTGGTTTCTG AAGACGATTC CCTTCTTGTG GCAGGCTTTG GAAGATTTGT GCGGATTTTC 
TTGGCTGACT CGCAAGGCTT CCACCGGTTC ATGGCTTCGG ATCTGGAACA CGAGGACATG 
GTGGAGACAG CAGTCTTGGG TCCTGAGAAC AATCTGATCA TCACAGGGTC CCGGGATGCA 
CTTATCCAGG TGTGGAGTTT GTCAGAACAG GGGACCCTGC TGAATGTCCT TGAGGGCGTG 
GGCGCCCCTG TGAGTCTGCT GGTCCGGGGT GGGACTCTGG TGGTGTCTGC ATCCCGGAAG 
TCTTCATCTT TCAAGGTCTG GGATCTCAAG TCCACTAAGA AGCTGCAGTC TCCGACTCCA 
TTTCTGGACC GCACCGGCCT CGCTGCAGTG TCCCACCATG GCAGCTTTGT CTACTTCCCT 
AAGGTTGGGG ACAAAAACAA AGTCACAATT TGGGACTTGG CAGAAGGTGA GGAGCAAGAT 
TGCCTGGACA CGTCCAATGA GGTCCGCTGT CTGGAGGTGG CCGAGCAGGC CAAGCTCCTC 
TTCACAGGCC TGGTTTCTGG GATCGTGCTT GTGTTCCCCC TGAATTCCAG GCAAGATGTA 
CTGTGCATCC CCCCACCTGA GGCACGCAAA GCTGTCAACT GCATGTCCCT GAGCAAGAGT 
GAGAATCGTC TGGCCATTGC CTATGACAAC ATTGTCCTGG TGCTTGACAT CAGCCCTGGG 
GACCCATGTC CGGCCATTGA AGGCCCCACC TACACCTTTT ACACCCAGCT GCCTGAGACC 
ATAGTGAGTG TGGCTGTCCT GGCTGACTAT CGTGTGGTCT ACGGCATGAG CGATGGCAGC 
CTCTTCCTGT ACGACTGTGC ATGCTCTAAA GTGTTCCCTC TGGAGGCCCA TGGGAGCAGG 
GTGAGCTGTG TAGAGGTCAG CCACAGTGAG CAGCTAGCAG TGAGCGGGGC TGAAGATGCA 
CTGCTATGCC TCTGGGACCT GCAGGCTTGC AGGGGGATGT TCGAGATGAG CTATGAGAAC 
TCCTGCTGCA GAGGGGTGCG GTGTGCCTGC TTCTCCAGGG ATGACAAGCA TGTATTTGCA 
GGCATGGAGG ACCGCTCTGT CACTGCCTGG AGCACGGTGG ATGGCACTCT TTTGGCTGTC 
CAGTTTGTTC ATGCTGTGAT CAACCGAATC ATCCCTACCT CCAATGGCTT CATGGCCCCC 
ACAAGCCACG GCTATCTCAT CCGTGAGCGC TTCCAGTGCC CGTCAGTCAG AGCCTCCCAG 
CAAGACCCTC TCAAGAACTT CAAGAAGGCA GTGTGGCTGG TAAAGACCAG GCAGAGGGAA 
GAGCTGGCTG CAGCAGAAGC CTCCCAGGAT GCAGAACCAG TGGCCGTAGA GGGAAAGGAA 
TCAAAGTCAA ATAAACGCTC ACAGGTCTGC CTCATACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531149.1
CDS	785..5506
Translation	MDTEREALQCTAYPEVQSFCQRHGLAFEVVDLRWGIPNTQATDYLTTELCLEELERCQKTSIGPAFVALLGDQYGPCPVPRRIEEKEWEALRAQLTSRPRDLELVTRHFQRDDNTIPPTYVLQPSGSLVVPGPEEATLTSVLRGGAQEAWRLGLISQEQWMCYHRSVIEWEIELGLLSSARGDQGATVFLRDVQDLNKHILDDCSLKMVDRLVDGCLDTNAQSLLSGLKGRILDAQPGALKSHHLSWSRDLVNPKNKAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPGIGKTSLMCKLAQQVPELLGHKTVVVLRLLGTSKLSLDARSLLRSLSFQVCLAYGLPLPPAQVLEAHSRVGHFFHTLLHTVSQRNFESLVLLLDSVDDLDSICHSPRVSWLPLKCPPRVHLILSTCSGQQVLHNLQQTLKDPSTYWEVKALSGSQGQEFIQLLLAAEKRMLSPGQRDVLWASLPECGHPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCIRLEETHGALLVAHVLGYIVSSRDLDRTWILWRYGLSEAELKDVLSLDDEVLQAVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLVRRPVDGCMLLAIAHRQLSQVIQVRYLSGPERAKRHGVLAEFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSSTVANLRKLTELPFHLLHAGRLEELKQEVLGNMSWISCRGISGGIEVLLDDFDMCAPHMNSPEVDLVREAIQLCGPAVELRGLEKSVLYTELLARLLFFAASHPAMIGQLCQQAQSWFRACPYPMLVPLAGFLQPPGGPLRATLTGCHKGITAIAWSLEEKLLVVGTQDGAMVVWDVEEQQVVHVLMGHTAEVKCVRVFAQGTLAISASKDHTLRLWSLLSGQEKVTILDGGSQNPTEPQSWDLHVDERNNVVYSTSGARINMWNLETSKLVFCITGDVSDPWVCVALLAAQGLLLALSKGGQVSLWSSAMGKLQEKHQLSSIKEETPTCAVSIQSRARLVAGFSSGSIALVSAGEDRLLEKLPEAVGFLVVSEDDSLLVAGFGRFVRIFLADSQGFHRFMASDLEHEDMVETAVLGPENNLIITGSRDALIQVWSLSEQGTLLNVLEGVGAPVSLLVRGGTLVVSASRKSSSFKVWDLKSTKKLQSPTPFLDRTGLAAVSHHGSFVYFPKVGDKNKVTIWDLAEGEEQDCLDTSNEVRCLEVAEQAKLLFTGLVSGIVLVFPLNSRQDVLCIPPPEARKAVNCMSLSKSENRLAIAYDNIVLVLDISPGDPCPAIEGPTYTFYTQLPETIVSVAVLADYRVVYGMSDGSLFLYDCACSKVFPLEAHGSRVSCVEVSHSEQLAVSGAEDALLCLWDLQACRGMFEMSYENSCCRGVRCACFSRDDKHVFAGMEDRSVTAWSTVDGTLLAVQFVHAVINRIIPTSNGFMAPTSHGYLIRERFQCPSVRASQQDPLKNFKKAVWLVKTRQREELAAAEASQDAEPVAVEGKESKSNKRSQVCLIL

Target ORF information:

RefSeq Version	XM_006531086.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531086.2

ORF Insert Sequence:

ATGGACACAG AGCGAGAAGC CCTGCAGTGC ACAGCCTACC CTGAGGTTCA GAGCTTCTGC 
CAAAGGCACG GCTTGGCGTT TGAGGTGGTT GATCTGAGGT GGGGTATTCC GAATACGCAG 
GCCACTGACT ACCTGACCAC GGAACTCTGC TTAGAAGAAC TGGAGCGCTG TCAGAAAACA 
TCCATAGGAC CAGCTTTTGT GGCCCTCCTG GGTGACCAGT ACGGCCCCTG CCCGGTTCCC 
CGGAGGATTG AGGAAAAGGA GTGGGAGGCA CTAAGAGCTC AGCTGACCTC CAGGCCTCGA 
GATCTGGAGC TGGTGACCAG ACATTTCCAG AGAGACGACA ACACCATTCC TCCAACCTAC 
GTCTTGCAGC CTTCAGGCTC ACTGGTGGTC CCAGGGCCTG AGGAGGCCAC CTTAACCTCT 
GTCCTGAGAG GTGGAGCCCA GGAGGCCTGG AGGCTAGGCC TCATCAGCCA GGAGCAATGG 
ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG GATTTGGACA GAACCTGGAT TTTGTGGAGG 
TACGGTCTCT CAGAAGCAGA GCTGAAGGAT GTGTTGTCTT TGGATGATGA AGTCTTGCAG 
GCTGTGTACC GGGACTGGAC GCCACCTAGC AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG 
TGGGTGCGGC TCCGCCGGGA CCTGGGACAC TGCCTAGTCA GGAGGCCCGT GGATGGCTGC 
ATGCTACTGG CCATTGCACA CAGACAGCTG TCCCAGGTAA TCCAAGTTCG ATATCTGTCT 
GGGCCTGAGA GAGCCAAGAG GCATGGCGTG CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC 
CAGGGGATCA AGAAACTTAT CACCCTGCCG CTTGTAGGGA AGCCCCTGAA CCTGGACCGC 
AAGGTGGCCC CACAGCCACT GTGGTTCTCA AGCACAGTGG CCAACCTGAG GAAGCTGACC 
GAACTGCCCT TCCATCTGCT GCATGCAGGC CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG 
GGGAACATGA GTTGGATCTC ATGTCGGGGC ATCTCTGGAG GCATTGAGGT CCTGCTGGAT 
GATTTTGACA TGTGTGCCCC TCACATGAAC TCTCCTGAGG TTGATCTGGT TAGAGAAGCC 
ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC CGGGGCCTGG AGAAGAGCGT CCTATACACC 
GAGCTCCTGG CCAGACTCCT TTTCTTTGCT GCATCACATC CAGCAATGAT CGGACAACTG 
TGCCAACAAG CCCAGAGCTG GTTCCGTGCC TGCCCCTACC CAATGCTGGT GCCCCTGGCA 
GGATTCCTGC AGCCACCTGG TGGACCTCTT CGAGCCACTC TTACTGGGTG TCACAAAGGC 
ATCACCGCCA TAGCATGGAG CCTGGAGGAG AAACTCCTGG TGGTGGGCAC CCAGGATGGT 
GCCATGGTCG TGTGGGACGT GGAGGAACAA CAGGTGGTAC ACGTTCTAAT GGGACACACA 
GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC CAAGGGACTC TTGCCATCTC TGCATCAAAG 
GACCATACCC TGCGTCTGTG GAGTCTGCTT TCCGGCCAGG AGAAAGTCAC CATTTTGGAT 
GGAGGGTCAC AAAATCCCAC AGAGCCCCAG AGTTGGGACC TTCATGTGGA TGAGAGAAAC 
AATGTTGTAT ACTCGACATC TGGTGCAAGG ATCAACATGT GGAATCTGGA AACTTCGAAG 
CTGGTTTTCT GTATCACAGG AGATGTGTCT GATCCCTGGG TCTGTGTGGC TTTGTTGGCT 
GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC 
ATGGGAAAAC TGCAGGAGAA ACACCAGTTA TCCAGCATCA AAGAAGAGAC ACCCACCTGT 
GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG GTCGCTGGCT TCAGCAGTGG CTCCATTGCC 
CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG 
GTGGTTTCTG AAGACGATTC CCTTCTTGTG GCAGGCTTTG GAAGATTTGT GCGGATTTTC 
TTGGCTGACT CGCAAGGCTT CCACCGGTTC ATGGCTTCGG ATCTGGAACA CGAGGACATG 
GTGGAGACAG CAGTCTTGGG TCCTGAGAAC AATCTGATCA TCACAGGGTC CCGGGATGCA 
CTTATCCAGG TGTGGAGTTT GTCAGAACAG GGGACCCTGC TGAATGTCCT TGAGGGCGTG 
GGCGCCCCTG TGAGTCTGCT GGTCCGGGGT GGGACTCTGG TGGTGTCTGC ATCCCGGAAG 
TCTTCATCTT TCAAGGTCTG GGATCTCAAG TCCACTAAGA AGCTGCAGTC TCCGACTCCA 
TTTCTGGACC GCACCGGCCT CGCTGCAGTG TCCCACCATG GCAGCTTTGT CTACTTCCCT 
AAGGTTGGGG ACAAAAACAA AGTCACAATT TGGGACTTGG CAGAAGGTGA GGAGCAAGAT 
TGCCTGGACA CGTCCAATGA GGTCCGCTGT CTGGAGGTGG CCGAGCAGGC CAAGCTCCTC 
TTCACAGGCC TGGTTTCTGG GATCGTGCTT GTGTTCCCCC TGAATTCCAG GCAAGATGTA 
CTGTGCATCC CCCCACCTGA GGCACGCAAA GCTGTCAACT GCATGTCCCT GAGCAAGAGT 
GAGAATCGTC TGGCCATTGC CTATGACAAC ATTGTCCTGG TGCTTGACAT CAGCCCTGGG 
GACCCATGTC CGGCCATTGA AGGCCCCACC TACACCTTTT ACACCCAGCT GCCTGAGACC 
ATAGTGAGTG TGGCTGTCCT GGCTGACTAT CGTGTGGTCT ACGGCATGAG CGATGGCAGC 
CTCTTCCTGT ACGACTGTGC ATGCTCTAAA GTGTTCCCTC TGGAGGCCCA TGGGAGCAGG 
GTGAGCTGTG TAGAGGTCAG CCACAGTGAG CAGCTAGCAG TGAGCGGGGC TGAAGATGCA 
CTGCTATGCC TCTGGGACCT GCAGGCTTGC AGGGGGATGT TCGAGATGAG CTATGAGAAC 
TCCTGCTGCA GAGGGGTGCG GTGTGCCTGC TTCTCCAGGG ATGACAAGCA TGTATTTGCA 
GGCATGGAGG ACCGCTCTGT CACTGCCTGG AGCACGGTGG ATGGCACTCT TTTGGCTGTC 
CAGTTTGTTC ATGCTGTGAT CAACCGAATC ATCCCTACCT CCAATGGCTT CATGGCCCCC 
ACAAGCCACG GCTATCTCAT CCGTGAGCGC TTCCAGTGCC CGTCAGTCAG AGCCTCCCAG 
CAAGACCCTC TCAAGAACTT CAAGAAGGCA GTGTGGCTGG TAAAGACCAG GCAGAGGGAA 
GAGCTGGCTG CAGCAGAAGC CTCCCAGGAT GCAGAACCAG TGGCCGTAGA GGGAAAGGAA 
TCAAAGTCAA ATAAACGCTC ACAGGTCTGC CTCATACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nwd1 ( XM_006531087.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531150.1 Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X2, mRNA.

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CloneID	OMu41531
Clone ID Related Accession (Same CDS sequence)	XM_006531087.4 , XM_006531087.3
Accession Version	XM_006531087.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531087.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAG AGCGAGAAGC CCTGCAGTGC ACAGCCTACC CTGAGGTTCA GAGCTTCTGC 
CAAAGGCACG GCTTGGCGTT TGAGGTGGTT GATCTGAGGT GGGGTATTCC GAATACGCAG 
GCCACTGACT ACCTGACCAC GGAACTCTGC TTAGAAGAAC TGGAGCGCTG TCAGAAAACA 
TCCATAGGAC CAGCTTTTGT GGCCCTCCTG GGTGACCAGT ACGGCCCCTG CCCGGTTCCC 
CGGAGGATTG AGGAAAAGGA GTGGGAGGCA CTAAGAGCTC AGCTGACCTC CAGGCCTCGA 
GATCTGGAGC TGGTGACCAG ACATTTCCAG AGAGACGACA ACACCATTCC TCCAACCTAC 
GTCTTGCAGC CTTCAGGCTC ACTGGTGGTC CCAGGGCCTG AGGAGGCCAC CTTAACCTCT 
GTCCTGAGAG GTGGAGCCCA GGAGGCCTGG AGGCTAGGCC TCATCAGCCA GGAGCAATGG 
ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG TACGGTCTCT CAGAAGCAGA GCTGAAGGAT 
GTGTTGTCTT TGGATGATGA AGTCTTGCAG GCTGTGTACC GGGACTGGAC GCCACCTAGC 
AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TCCGCCGGGA CCTGGGACAC 
TGCCTAGTCA GGAGGCCCGT GGATGGCTGC ATGCTACTGG CCATTGCACA CAGACAGCTG 
TCCCAGGTAA TCCAAGTTCG ATATCTGTCT GGGCCTGAGA GAGCCAAGAG GCATGGCGTG 
CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC CAGGGGATCA AGAAACTTAT CACCCTGCCG 
CTTGTAGGGA AGCCCCTGAA CCTGGACCGC AAGGTGGCCC CACAGCCACT GTGGTTCTCA 
AGCACAGTGG CCAACCTGAG GAAGCTGACC GAACTGCCCT TCCATCTGCT GCATGCAGGC 
CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG GGGAACATGA GTTGGATCTC ATGTCGGGGC 
ATCTCTGGAG GCATTGAGGT CCTGCTGGAT GATTTTGACA TGTGTGCCCC TCACATGAAC 
TCTCCTGAGG TTGATCTGGT TAGAGAAGCC ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC 
CGGGGCCTGG AGAAGAGCGT CCTATACACC GAGCTCCTGG CCAGACTCCT TTTCTTTGCT 
GCATCACATC CAGCAATGAT CGGACAACTG TGCCAACAAG CCCAGAGCTG GTTCCGTGCC 
TGCCCCTACC CAATGCTGGT GCCCCTGGCA GGATTCCTGC AGCCACCTGG TGGACCTCTT 
CGAGCCACTC TTACTGGGTG TCACAAAGGC ATCACCGCCA TAGCATGGAG CCTGGAGGAG 
AAACTCCTGG TGGTGGGCAC CCAGGATGGT GCCATGGTCG TGTGGGACGT GGAGGAACAA 
CAGGTGGTAC ACGTTCTAAT GGGACACACA GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC 
CAAGGGACTC TTGCCATCTC TGCATCAAAG GACCATACCC TGCGTCTGTG GAGTCTGCTT 
TCCGGCCAGG AGAAAGTCAC CATTTTGGAT GGAGGGTCAC AAAATCCCAC AGAGCCCCAG 
AGTTGGGACC TTCATGTGGA TGAGAGAAAC AATGTTGTAT ACTCGACATC TGGTGCAAGG 
ATCAACATGT GGAATCTGGA AACTTCGAAG CTGGTTTTCT GTATCACAGG AGATGTGTCT 
GATCCCTGGG TCTGTGTGGC TTTGTTGGCT GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG 
GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC ATGGGAAAAC TGCAGGAGAA ACACCAGTTA 
TCCAGCATCA AAGAAGAGAC ACCCACCTGT GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG 
GTCGCTGGCT TCAGCAGTGG CTCCATTGCC CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG 
GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG GTGGTTTCTG AAGACGATTC CCTTCTTGTG 
GCAGGCTTTG GAAGATTTGT GCGGATTTTC TTGGCTGACT CGCAAGGCTT CCACCGGTTC 
ATGGCTTCGG ATCTGGAACA CGAGGACATG GTGGAGACAG CAGTCTTGGG TCCTGAGAAC 
AATCTGATCA TCACAGGGTC CCGGGATGCA CTTATCCAGG TGTGGAGTTT GTCAGAACAG 
GGGACCCTGC TGAATGTCCT TGAGGGCGTG GGCGCCCCTG TGAGTCTGCT GGTCCGGGGT 
GGGACTCTGG TGGTGTCTGC ATCCCGGAAG TCTTCATCTT TCAAGGTCTG GGATCTCAAG 
TCCACTAAGA AGCTGCAGTC TCCGACTCCA TTTCTGGACC GCACCGGCCT CGCTGCAGTG 
TCCCACCATG GCAGCTTTGT CTACTTCCCT AAGGTTGGGG ACAAAAACAA AGTCACAATT 
TGGGACTTGG CAGAAGGTGA GGAGCAAGAT TGCCTGGACA CGTCCAATGA GGTCCGCTGT 
CTGGAGGTGG CCGAGCAGGC CAAGCTCCTC TTCACAGGCC TGGTTTCTGG GATCGTGCTT 
GTGTTCCCCC TGAATTCCAG GCAAGATGTA CTGTGCATCC CCCCACCTGA GGCACGCAAA 
GCTGTCAACT GCATGTCCCT GAGCAAGAGT GAGAATCGTC TGGCCATTGC CTATGACAAC 
ATTGTCCTGG TGCTTGACAT CAGCCCTGGG GACCCATGTC CGGCCATTGA AGGCCCCACC 
TACACCTTTT ACACCCAGCT GCCTGAGACC ATAGTGAGTG TGGCTGTCCT GGCTGACTAT 
CGTGTGGTCT ACGGCATGAG CGATGGCAGC CTCTTCCTGT ACGACTGTGC ATGCTCTAAA 
GTGTTCCCTC TGGAGGCCCA TGGGAGCAGG GTGAGCTGTG TAGAGGTCAG CCACAGTGAG 
CAGCTAGCAG TGAGCGGGGC TGAAGATGCA CTGCTATGCC TCTGGGACCT GCAGGCTTGC 
AGGGGGATGT TCGAGATGAG CTATGAGAAC TCCTGCTGCA GAGGGGTGCG GTGTGCCTGC 
TTCTCCAGGG ATGACAAGCA TGTATTTGCA GGCATGGAGG ACCGCTCTGT CACTGCCTGG 
AGCACGGTGG ATGGCACTCT TTTGGCTGTC CAGTTTGTTC ATGCTGTGAT CAACCGAATC 
ATCCCTACCT CCAATGGCTT CATGGCCCCC ACAAGCCACG GCTATCTCAT CCGTGAGCGC 
TTCCAGTGCC CGTCAGTCAG AGCCTCCCAG CAAGACCCTC TCAAGAACTT CAAGAAGGCA 
GTGTGGCTGG TAAAGACCAG GCAGAGGGAA GAGCTGGCTG CAGCAGAAGC CTCCCAGGAT 
GCAGAACCAG TGGCCGTAGA GGGAAAGGAA TCAAAGTCAA ATAAACGCTC ACAGGTCTGC 
CTCATACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531150.1
CDS	784..5475
Translation	MDTEREALQCTAYPEVQSFCQRHGLAFEVVDLRWGIPNTQATDYLTTELCLEELERCQKTSIGPAFVALLGDQYGPCPVPRRIEEKEWEALRAQLTSRPRDLELVTRHFQRDDNTIPPTYVLQPSGSLVVPGPEEATLTSVLRGGAQEAWRLGLISQEQWMCYHRSVIEWEIELGLLSSARGDQGATVFLRDVQDLNKHILDDCSLKMVDRLVDGCLDTNAQSLLSGLKGRILDAQPGALKSHHLSWSRDLVNPKNKAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPGIGKTSLMCKLAQQVPELLGHKTVVVLRLLGTSKLSLDARSLLRSLSFQVCLAYGLPLPPAQVLEAHSRVGHFFHTLLHTVSQRNFESLVLLLDSVDDLDSICHSPRVSWLPLKCPPRVHLILSTCSGQQVLHNLQQTLKDPSTYWEVKALSGSQGQEFIQLLLAAEKRMLSPGQRDVLWASLPECGHPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCIRLEETHGALLVAHVLGYIVSSRYGLSEAELKDVLSLDDEVLQAVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLVRRPVDGCMLLAIAHRQLSQVIQVRYLSGPERAKRHGVLAEFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSSTVANLRKLTELPFHLLHAGRLEELKQEVLGNMSWISCRGISGGIEVLLDDFDMCAPHMNSPEVDLVREAIQLCGPAVELRGLEKSVLYTELLARLLFFAASHPAMIGQLCQQAQSWFRACPYPMLVPLAGFLQPPGGPLRATLTGCHKGITAIAWSLEEKLLVVGTQDGAMVVWDVEEQQVVHVLMGHTAEVKCVRVFAQGTLAISASKDHTLRLWSLLSGQEKVTILDGGSQNPTEPQSWDLHVDERNNVVYSTSGARINMWNLETSKLVFCITGDVSDPWVCVALLAAQGLLLALSKGGQVSLWSSAMGKLQEKHQLSSIKEETPTCAVSIQSRARLVAGFSSGSIALVSAGEDRLLEKLPEAVGFLVVSEDDSLLVAGFGRFVRIFLADSQGFHRFMASDLEHEDMVETAVLGPENNLIITGSRDALIQVWSLSEQGTLLNVLEGVGAPVSLLVRGGTLVVSASRKSSSFKVWDLKSTKKLQSPTPFLDRTGLAAVSHHGSFVYFPKVGDKNKVTIWDLAEGEEQDCLDTSNEVRCLEVAEQAKLLFTGLVSGIVLVFPLNSRQDVLCIPPPEARKAVNCMSLSKSENRLAIAYDNIVLVLDISPGDPCPAIEGPTYTFYTQLPETIVSVAVLADYRVVYGMSDGSLFLYDCACSKVFPLEAHGSRVSCVEVSHSEQLAVSGAEDALLCLWDLQACRGMFEMSYENSCCRGVRCACFSRDDKHVFAGMEDRSVTAWSTVDGTLLAVQFVHAVINRIIPTSNGFMAPTSHGYLIRERFQCPSVRASQQDPLKNFKKAVWLVKTRQREELAAAEASQDAEPVAVEGKESKSNKRSQVCLIL

Target ORF information:

RefSeq Version	XM_006531087.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531087.3

ORF Insert Sequence:

ATGGACACAG AGCGAGAAGC CCTGCAGTGC ACAGCCTACC CTGAGGTTCA GAGCTTCTGC 
CAAAGGCACG GCTTGGCGTT TGAGGTGGTT GATCTGAGGT GGGGTATTCC GAATACGCAG 
GCCACTGACT ACCTGACCAC GGAACTCTGC TTAGAAGAAC TGGAGCGCTG TCAGAAAACA 
TCCATAGGAC CAGCTTTTGT GGCCCTCCTG GGTGACCAGT ACGGCCCCTG CCCGGTTCCC 
CGGAGGATTG AGGAAAAGGA GTGGGAGGCA CTAAGAGCTC AGCTGACCTC CAGGCCTCGA 
GATCTGGAGC TGGTGACCAG ACATTTCCAG AGAGACGACA ACACCATTCC TCCAACCTAC 
GTCTTGCAGC CTTCAGGCTC ACTGGTGGTC CCAGGGCCTG AGGAGGCCAC CTTAACCTCT 
GTCCTGAGAG GTGGAGCCCA GGAGGCCTGG AGGCTAGGCC TCATCAGCCA GGAGCAATGG 
ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG TACGGTCTCT CAGAAGCAGA GCTGAAGGAT 
GTGTTGTCTT TGGATGATGA AGTCTTGCAG GCTGTGTACC GGGACTGGAC GCCACCTAGC 
AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG TGGGTGCGGC TCCGCCGGGA CCTGGGACAC 
TGCCTAGTCA GGAGGCCCGT GGATGGCTGC ATGCTACTGG CCATTGCACA CAGACAGCTG 
TCCCAGGTAA TCCAAGTTCG ATATCTGTCT GGGCCTGAGA GAGCCAAGAG GCATGGCGTG 
CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC CAGGGGATCA AGAAACTTAT CACCCTGCCG 
CTTGTAGGGA AGCCCCTGAA CCTGGACCGC AAGGTGGCCC CACAGCCACT GTGGTTCTCA 
AGCACAGTGG CCAACCTGAG GAAGCTGACC GAACTGCCCT TCCATCTGCT GCATGCAGGC 
CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG GGGAACATGA GTTGGATCTC ATGTCGGGGC 
ATCTCTGGAG GCATTGAGGT CCTGCTGGAT GATTTTGACA TGTGTGCCCC TCACATGAAC 
TCTCCTGAGG TTGATCTGGT TAGAGAAGCC ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC 
CGGGGCCTGG AGAAGAGCGT CCTATACACC GAGCTCCTGG CCAGACTCCT TTTCTTTGCT 
GCATCACATC CAGCAATGAT CGGACAACTG TGCCAACAAG CCCAGAGCTG GTTCCGTGCC 
TGCCCCTACC CAATGCTGGT GCCCCTGGCA GGATTCCTGC AGCCACCTGG TGGACCTCTT 
CGAGCCACTC TTACTGGGTG TCACAAAGGC ATCACCGCCA TAGCATGGAG CCTGGAGGAG 
AAACTCCTGG TGGTGGGCAC CCAGGATGGT GCCATGGTCG TGTGGGACGT GGAGGAACAA 
CAGGTGGTAC ACGTTCTAAT GGGACACACA GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC 
CAAGGGACTC TTGCCATCTC TGCATCAAAG GACCATACCC TGCGTCTGTG GAGTCTGCTT 
TCCGGCCAGG AGAAAGTCAC CATTTTGGAT GGAGGGTCAC AAAATCCCAC AGAGCCCCAG 
AGTTGGGACC TTCATGTGGA TGAGAGAAAC AATGTTGTAT ACTCGACATC TGGTGCAAGG 
ATCAACATGT GGAATCTGGA AACTTCGAAG CTGGTTTTCT GTATCACAGG AGATGTGTCT 
GATCCCTGGG TCTGTGTGGC TTTGTTGGCT GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG 
GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC ATGGGAAAAC TGCAGGAGAA ACACCAGTTA 
TCCAGCATCA AAGAAGAGAC ACCCACCTGT GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG 
GTCGCTGGCT TCAGCAGTGG CTCCATTGCC CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG 
GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG GTGGTTTCTG AAGACGATTC CCTTCTTGTG 
GCAGGCTTTG GAAGATTTGT GCGGATTTTC TTGGCTGACT CGCAAGGCTT CCACCGGTTC 
ATGGCTTCGG ATCTGGAACA CGAGGACATG GTGGAGACAG CAGTCTTGGG TCCTGAGAAC 
AATCTGATCA TCACAGGGTC CCGGGATGCA CTTATCCAGG TGTGGAGTTT GTCAGAACAG 
GGGACCCTGC TGAATGTCCT TGAGGGCGTG GGCGCCCCTG TGAGTCTGCT GGTCCGGGGT 
GGGACTCTGG TGGTGTCTGC ATCCCGGAAG TCTTCATCTT TCAAGGTCTG GGATCTCAAG 
TCCACTAAGA AGCTGCAGTC TCCGACTCCA TTTCTGGACC GCACCGGCCT CGCTGCAGTG 
TCCCACCATG GCAGCTTTGT CTACTTCCCT AAGGTTGGGG ACAAAAACAA AGTCACAATT 
TGGGACTTGG CAGAAGGTGA GGAGCAAGAT TGCCTGGACA CGTCCAATGA GGTCCGCTGT 
CTGGAGGTGG CCGAGCAGGC CAAGCTCCTC TTCACAGGCC TGGTTTCTGG GATCGTGCTT 
GTGTTCCCCC TGAATTCCAG GCAAGATGTA CTGTGCATCC CCCCACCTGA GGCACGCAAA 
GCTGTCAACT GCATGTCCCT GAGCAAGAGT GAGAATCGTC TGGCCATTGC CTATGACAAC 
ATTGTCCTGG TGCTTGACAT CAGCCCTGGG GACCCATGTC CGGCCATTGA AGGCCCCACC 
TACACCTTTT ACACCCAGCT GCCTGAGACC ATAGTGAGTG TGGCTGTCCT GGCTGACTAT 
CGTGTGGTCT ACGGCATGAG CGATGGCAGC CTCTTCCTGT ACGACTGTGC ATGCTCTAAA 
GTGTTCCCTC TGGAGGCCCA TGGGAGCAGG GTGAGCTGTG TAGAGGTCAG CCACAGTGAG 
CAGCTAGCAG TGAGCGGGGC TGAAGATGCA CTGCTATGCC TCTGGGACCT GCAGGCTTGC 
AGGGGGATGT TCGAGATGAG CTATGAGAAC TCCTGCTGCA GAGGGGTGCG GTGTGCCTGC 
TTCTCCAGGG ATGACAAGCA TGTATTTGCA GGCATGGAGG ACCGCTCTGT CACTGCCTGG 
AGCACGGTGG ATGGCACTCT TTTGGCTGTC CAGTTTGTTC ATGCTGTGAT CAACCGAATC 
ATCCCTACCT CCAATGGCTT CATGGCCCCC ACAAGCCACG GCTATCTCAT CCGTGAGCGC 
TTCCAGTGCC CGTCAGTCAG AGCCTCCCAG CAAGACCCTC TCAAGAACTT CAAGAAGGCA 
GTGTGGCTGG TAAAGACCAG GCAGAGGGAA GAGCTGGCTG CAGCAGAAGC CTCCCAGGAT 
GCAGAACCAG TGGCCGTAGA GGGAAAGGAA TCAAAGTCAA ATAAACGCTC ACAGGTCTGC 
CTCATACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nwd1 ( XM_006531088.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531151.1 Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X5, mRNA.

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CloneID	OMu41532
Clone ID Related Accession (Same CDS sequence)	XM_006531088.3 , XM_006531089.2
Accession Version	XM_006531088.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531088.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATC GGCTGGTAGA TGGCTGCCTG GATACCAATG CCCAGAGTCT TCTCAGTGGT 
CTTAAAGGGC GCATACTAGA TGCTCAGCCT GGAGCCCTAA AGTCCCACCA CCTGTCCTGG 
AGCCGAGACC TGGTCAACCC CAAGAACAAG GCTCACGCTC GTTACCTAAA GCAGTTGAGC 
GAGCAGTTCG TGGCCAGGAC CAACCATCAG GTCCTGGAGC AGCTTCGAGA GCTGGAGTTG 
GCCAGACAGG AGCTGGGTTG GCTCTACCAA GAGATCCGCC ACCACCTCTG GCAGAGCACA 
GAGTCTACCA AGGTCTTCTG CGGCCACCAG GAGCTCCTGG CCCAGCTCAG GCAGCAGCTC 
AGACAGGATG AGAGCCGGAC ACATACTCCC TTGGTTCTCT TTGGACCACC AGGCATCGGG 
AAGACCTCTC TCATGTGCAA GCTAGCCCAG CAGGTACCAG AGCTACTTGG CCACAAGACA 
GTGGTTGTCC TGAGGCTGCT AGGGACATCA AAGCTGAGCC TGGATGCCCG CAGCCTCCTC 
AGGAGCCTGT CCTTCCAAGT GTGCTTGGCA TATGGGCTGC CACTGCCCCC AGCCCAGGTC 
CTGGAGGCCC ACAGCAGGGT GGGCCATTTC TTCCATACTC TCCTCCACAC CGTGTCACAG 
AGGAACTTTG AGTCCCTGGT GTTGCTCCTG GACTCCGTGG ATGACCTGGA CTCCATATGC 
CATTCTCCGA GGGTCTCTTG GCTGCCCCTC AAGTGTCCTC CAAGGGTGCA CCTCATCCTC 
TCTACATGCT CAGGCCAGCA GGTTTTACAT AACCTGCAAC AGACCCTCAA GGACCCCAGC 
ACCTACTGGG AGGTGAAGGC CCTGTCTGGA AGTCAAGGCC AGGAGTTCAT ACAGCTGCTG 
CTGGCTGCAG AGAAGAGGAT GCTGAGCCCG GGTCAGAGAG ATGTCCTCTG GGCCAGCCTC 
CCAGAGTGTG GCCACCCAGG GAGGCTAAGG CTGGCCTTTG AGGAGGCCCG GAAGTGGGCC 
TCCTTCACTG TGCCAGTGCC CTTGGCCACT ACAGCAGAGG AAGCCACACA CCAGCTCTGT 
ATACGCCTGG AGGAGACACA TGGGGCACTG CTGGTGGCTC ATGTACTGGG CTATATTGTG 
TCTTCCAGGG ATTTGGACAG AACCTGGATT TTGTGGAGGT ACGGTCTCTC AGAAGCAGAG 
CTGAAGGATG TGTTGTCTTT GGATGATGAA GTCTTGCAGG CTGTGTACCG GGACTGGACG 
CCACCTAGCA AAGAGCTGCT GCGCTTCCCG CCCCTGCTGT GGGTGCGGCT CCGCCGGGAC 
CTGGGACACT GCCTAGTCAG GAGGCCCGTG GATGGCTGCA TGCTACTGGC CATTGCACAC 
AGACAGCTGT CCCAGGTAAT CCAAGTTCGA TATCTGTCTG GGCCTGAGAG AGCCAAGAGG 
CATGGCGTGC TAGCTGAATT CTTCTCTGGA GCCTGGAGCC AGGGGATCAA GAAACTTATC 
ACCCTGCCGC TTGTAGGGAA GCCCCTGAAC CTGGACCGCA AGGTGGCCCC ACAGCCACTG 
TGGTTCTCAA GCACAGTGGC CAACCTGAGG AAGCTGACCG AACTGCCCTT CCATCTGCTG 
CATGCAGGCC GCTTGGAGGA GTTGAAGCAG GAGGTTCTGG GGAACATGAG TTGGATCTCA 
TGTCGGGGCA TCTCTGGAGG CATTGAGGTC CTGCTGGATG ATTTTGACAT GTGTGCCCCT 
CACATGAACT CTCCTGAGGT TGATCTGGTT AGAGAAGCCA TCCAGCTGTG TGGCCCTGCT 
GTGGAGCTCC GGGGCCTGGA GAAGAGCGTC CTATACACCG AGCTCCTGGC CAGACTCCTT 
TTCTTTGCTG CATCACATCC AGCAATGATC GGACAACTGT GCCAACAAGC CCAGAGCTGG 
TTCCGTGCCT GCCCCTACCC AATGCTGGTG CCCCTGGCAG GATTCCTGCA GCCACCTGGT 
GGACCTCTTC GAGCCACTCT TACTGGGTGT CACAAAGGCA TCACCGCCAT AGCATGGAGC 
CTGGAGGAGA AACTCCTGGT GGTGGGCACC CAGGATGGTG CCATGGTCGT GTGGGACGTG 
GAGGAACAAC AGGTGGTACA CGTTCTAATG GGACACACAG CTGAAGTGAA GTGTGTGCGA 
GTGTTTGCCC AAGGGACTCT TGCCATCTCT GCATCAAAGG ACCATACCCT GCGTCTGTGG 
AGTCTGCTTT CCGGCCAGGA GAAAGTCACC ATTTTGGATG GAGGGTCACA AAATCCCACA 
GAGCCCCAGA GTTGGGACCT TCATGTGGAT GAGAGAAACA ATGTTGTATA CTCGACATCT 
GGTGCAAGGA TCAACATGTG GAATCTGGAA ACTTCGAAGC TGGTTTTCTG TATCACAGGA 
GATGTGTCTG ATCCCTGGGT CTGTGTGGCT TTGTTGGCTG CCCAGGGTTT GCTGCTTGCC 
CTGTCCAAGG GTGGCCAGGT CAGTCTGTGG AGCTCAGCCA TGGGAAAACT GCAGGAGAAA 
CACCAGTTAT CCAGCATCAA AGAAGAGACA CCCACCTGTG CCGTCTCCAT CCAGTCCCGG 
GCAAGGCTGG TCGCTGGCTT CAGCAGTGGC TCCATTGCCC TGGTCTCCGC TGGAGAAGAC 
AGGCTGCTGG AGAAGCTCCC AGAAGCTGTG GGGTTCCTGG TGGTTTCTGA AGACGATTCC 
CTTCTTGTGG CAGGCTTTGG AAGATTTGTG CGGATTTTCT TGGCTGACTC GCAAGGCTTC 
CACCGGTTCA TGGCTTCGGA TCTGGAACAC GAGGACATGG TGGAGACAGC AGTCTTGGGT 
CCTGAGAACA ATCTGATCAT CACAGGGTCC CGGGATGCAC TTATCCAGGT GTGGAGTTTG 
TCAGAACAGG GGACCCTGCT GAATGTCCTT GAGGGCGTGG GCGCCCCTGT GAGTCTGCTG 
GTCCGGGGTG GGACTCTGGT GGTGTCTGCA TCCCGGAAGT CTTCATCTTT CAAGGTCTGG 
GATCTCAAGT CCACTAAGAA GCTGCAGTCT CCGACTCCAT TTCTGGACCG CACCGGCCTC 
GCTGCAGTGT CCCACCATGG CAGCTTTGTC TACTTCCCTA AGGTTGGGGA CAAAAACAAA 
GTCACAATTT GGGACTTGGC AGAAGGTGAG GAGCAAGATT GCCTGGACAC GTCCAATGAG 
GTCCGCTGTC TGGAGGTGGC CGAGCAGGCC AAGCTCCTCT TCACAGGCCT GGTTTCTGGG 
ATCGTGCTTG TGTTCCCCCT GAATTCCAGG CAAGATGTAC TGTGCATCCC CCCACCTGAG 
GCACGCAAAG CTGTCAACTG CATGTCCCTG AGCAAGAGTG AGAATCGTCT GGCCATTGCC 
TATGACAACA TTGTCCTGGT GCTTGACATC AGCCCTGGGG ACCCATGTCC GGCCATTGAA 
GGCCCCACCT ACACCTTTTA CACCCAGCTG CCTGAGACCA TAGTGAGTGT GGCTGTCCTG 
GCTGACTATC GTGTGGTCTA CGGCATGAGC GATGGCAGCC TCTTCCTGTA CGACTGTGCA 
TGCTCTAAAG TGTTCCCTCT GGAGGCCCAT GGGAGCAGGG TGAGCTGTGT AGAGGTCAGC 
CACAGTGAGC AGCTAGCAGT GAGCGGGGCT GAAGATGCAC TGCTATGCCT CTGGGACCTG 
CAGGCTTGCA GGGGGATGTT CGAGATGAGC TATGAGAACT CCTGCTGCAG AGGGGTGCGG 
TGTGCCTGCT TCTCCAGGGA TGACAAGCAT GTATTTGCAG GCATGGAGGA CCGCTCTGTC 
ACTGCCTGGA GCACGGTGGA TGGCACTCTT TTGGCTGTCC AGTTTGTTCA TGCTGTGATC 
AACCGAATCA TCCCTACCTC CAATGGCTTC ATGGCCCCCA CAAGCCACGG CTATCTCATC 
CGTGAGCGCT TCCAGTGCCC GTCAGTCAGA GCCTCCCAGC AAGACCCTCT CAAGAACTTC 
AAGAAGGCAG TGTGGCTGGT AAAGACCAGG CAGAGGGAAG AGCTGGCTGC AGCAGAAGCC 
TCCCAGGATG CAGAACCAGT GGCCGTAGAG GGAAAGGAAT CAAAGTCAAA TAAACGCTCA 
CAGGTCTGCC TCATACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531151.1
CDS	1107..5207
Translation	MVDRLVDGCLDTNAQSLLSGLKGRILDAQPGALKSHHLSWSRDLVNPKNKAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPGIGKTSLMCKLAQQVPELLGHKTVVVLRLLGTSKLSLDARSLLRSLSFQVCLAYGLPLPPAQVLEAHSRVGHFFHTLLHTVSQRNFESLVLLLDSVDDLDSICHSPRVSWLPLKCPPRVHLILSTCSGQQVLHNLQQTLKDPSTYWEVKALSGSQGQEFIQLLLAAEKRMLSPGQRDVLWASLPECGHPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCIRLEETHGALLVAHVLGYIVSSRDLDRTWILWRYGLSEAELKDVLSLDDEVLQAVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLVRRPVDGCMLLAIAHRQLSQVIQVRYLSGPERAKRHGVLAEFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSSTVANLRKLTELPFHLLHAGRLEELKQEVLGNMSWISCRGISGGIEVLLDDFDMCAPHMNSPEVDLVREAIQLCGPAVELRGLEKSVLYTELLARLLFFAASHPAMIGQLCQQAQSWFRACPYPMLVPLAGFLQPPGGPLRATLTGCHKGITAIAWSLEEKLLVVGTQDGAMVVWDVEEQQVVHVLMGHTAEVKCVRVFAQGTLAISASKDHTLRLWSLLSGQEKVTILDGGSQNPTEPQSWDLHVDERNNVVYSTSGARINMWNLETSKLVFCITGDVSDPWVCVALLAAQGLLLALSKGGQVSLWSSAMGKLQEKHQLSSIKEETPTCAVSIQSRARLVAGFSSGSIALVSAGEDRLLEKLPEAVGFLVVSEDDSLLVAGFGRFVRIFLADSQGFHRFMASDLEHEDMVETAVLGPENNLIITGSRDALIQVWSLSEQGTLLNVLEGVGAPVSLLVRGGTLVVSASRKSSSFKVWDLKSTKKLQSPTPFLDRTGLAAVSHHGSFVYFPKVGDKNKVTIWDLAEGEEQDCLDTSNEVRCLEVAEQAKLLFTGLVSGIVLVFPLNSRQDVLCIPPPEARKAVNCMSLSKSENRLAIAYDNIVLVLDISPGDPCPAIEGPTYTFYTQLPETIVSVAVLADYRVVYGMSDGSLFLYDCACSKVFPLEAHGSRVSCVEVSHSEQLAVSGAEDALLCLWDLQACRGMFEMSYENSCCRGVRCACFSRDDKHVFAGMEDRSVTAWSTVDGTLLAVQFVHAVINRIIPTSNGFMAPTSHGYLIRERFQCPSVRASQQDPLKNFKKAVWLVKTRQREELAAAEASQDAEPVAVEGKESKSNKRSQVCLIL

Target ORF information:

RefSeq Version	XM_006531088.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531088.3

ORF Insert Sequence:

ATGGTGGATC GGCTGGTAGA TGGCTGCCTG GATACCAATG CCCAGAGTCT TCTCAGTGGT 
CTTAAAGGGC GCATACTAGA TGCTCAGCCT GGAGCCCTAA AGTCCCACCA CCTGTCCTGG 
AGCCGAGACC TGGTCAACCC CAAGAACAAG GCTCACGCTC GTTACCTAAA GCAGTTGAGC 
GAGCAGTTCG TGGCCAGGAC CAACCATCAG GTCCTGGAGC AGCTTCGAGA GCTGGAGTTG 
GCCAGACAGG AGCTGGGTTG GCTCTACCAA GAGATCCGCC ACCACCTCTG GCAGAGCACA 
GAGTCTACCA AGGTCTTCTG CGGCCACCAG GAGCTCCTGG CCCAGCTCAG GCAGCAGCTC 
AGACAGGATG AGAGCCGGAC ACATACTCCC TTGGTTCTCT TTGGACCACC AGGCATCGGG 
AAGACCTCTC TCATGTGCAA GCTAGCCCAG CAGGTACCAG AGCTACTTGG CCACAAGACA 
GTGGTTGTCC TGAGGCTGCT AGGGACATCA AAGCTGAGCC TGGATGCCCG CAGCCTCCTC 
AGGAGCCTGT CCTTCCAAGT GTGCTTGGCA TATGGGCTGC CACTGCCCCC AGCCCAGGTC 
CTGGAGGCCC ACAGCAGGGT GGGCCATTTC TTCCATACTC TCCTCCACAC CGTGTCACAG 
AGGAACTTTG AGTCCCTGGT GTTGCTCCTG GACTCCGTGG ATGACCTGGA CTCCATATGC 
CATTCTCCGA GGGTCTCTTG GCTGCCCCTC AAGTGTCCTC CAAGGGTGCA CCTCATCCTC 
TCTACATGCT CAGGCCAGCA GGTTTTACAT AACCTGCAAC AGACCCTCAA GGACCCCAGC 
ACCTACTGGG AGGTGAAGGC CCTGTCTGGA AGTCAAGGCC AGGAGTTCAT ACAGCTGCTG 
CTGGCTGCAG AGAAGAGGAT GCTGAGCCCG GGTCAGAGAG ATGTCCTCTG GGCCAGCCTC 
CCAGAGTGTG GCCACCCAGG GAGGCTAAGG CTGGCCTTTG AGGAGGCCCG GAAGTGGGCC 
TCCTTCACTG TGCCAGTGCC CTTGGCCACT ACAGCAGAGG AAGCCACACA CCAGCTCTGT 
ATACGCCTGG AGGAGACACA TGGGGCACTG CTGGTGGCTC ATGTACTGGG CTATATTGTG 
TCTTCCAGGG ATTTGGACAG AACCTGGATT TTGTGGAGGT ACGGTCTCTC AGAAGCAGAG 
CTGAAGGATG TGTTGTCTTT GGATGATGAA GTCTTGCAGG CTGTGTACCG GGACTGGACG 
CCACCTAGCA AAGAGCTGCT GCGCTTCCCG CCCCTGCTGT GGGTGCGGCT CCGCCGGGAC 
CTGGGACACT GCCTAGTCAG GAGGCCCGTG GATGGCTGCA TGCTACTGGC CATTGCACAC 
AGACAGCTGT CCCAGGTAAT CCAAGTTCGA TATCTGTCTG GGCCTGAGAG AGCCAAGAGG 
CATGGCGTGC TAGCTGAATT CTTCTCTGGA GCCTGGAGCC AGGGGATCAA GAAACTTATC 
ACCCTGCCGC TTGTAGGGAA GCCCCTGAAC CTGGACCGCA AGGTGGCCCC ACAGCCACTG 
TGGTTCTCAA GCACAGTGGC CAACCTGAGG AAGCTGACCG AACTGCCCTT CCATCTGCTG 
CATGCAGGCC GCTTGGAGGA GTTGAAGCAG GAGGTTCTGG GGAACATGAG TTGGATCTCA 
TGTCGGGGCA TCTCTGGAGG CATTGAGGTC CTGCTGGATG ATTTTGACAT GTGTGCCCCT 
CACATGAACT CTCCTGAGGT TGATCTGGTT AGAGAAGCCA TCCAGCTGTG TGGCCCTGCT 
GTGGAGCTCC GGGGCCTGGA GAAGAGCGTC CTATACACCG AGCTCCTGGC CAGACTCCTT 
TTCTTTGCTG CATCACATCC AGCAATGATC GGACAACTGT GCCAACAAGC CCAGAGCTGG 
TTCCGTGCCT GCCCCTACCC AATGCTGGTG CCCCTGGCAG GATTCCTGCA GCCACCTGGT 
GGACCTCTTC GAGCCACTCT TACTGGGTGT CACAAAGGCA TCACCGCCAT AGCATGGAGC 
CTGGAGGAGA AACTCCTGGT GGTGGGCACC CAGGATGGTG CCATGGTCGT GTGGGACGTG 
GAGGAACAAC AGGTGGTACA CGTTCTAATG GGACACACAG CTGAAGTGAA GTGTGTGCGA 
GTGTTTGCCC AAGGGACTCT TGCCATCTCT GCATCAAAGG ACCATACCCT GCGTCTGTGG 
AGTCTGCTTT CCGGCCAGGA GAAAGTCACC ATTTTGGATG GAGGGTCACA AAATCCCACA 
GAGCCCCAGA GTTGGGACCT TCATGTGGAT GAGAGAAACA ATGTTGTATA CTCGACATCT 
GGTGCAAGGA TCAACATGTG GAATCTGGAA ACTTCGAAGC TGGTTTTCTG TATCACAGGA 
GATGTGTCTG ATCCCTGGGT CTGTGTGGCT TTGTTGGCTG CCCAGGGTTT GCTGCTTGCC 
CTGTCCAAGG GTGGCCAGGT CAGTCTGTGG AGCTCAGCCA TGGGAAAACT GCAGGAGAAA 
CACCAGTTAT CCAGCATCAA AGAAGAGACA CCCACCTGTG CCGTCTCCAT CCAGTCCCGG 
GCAAGGCTGG TCGCTGGCTT CAGCAGTGGC TCCATTGCCC TGGTCTCCGC TGGAGAAGAC 
AGGCTGCTGG AGAAGCTCCC AGAAGCTGTG GGGTTCCTGG TGGTTTCTGA AGACGATTCC 
CTTCTTGTGG CAGGCTTTGG AAGATTTGTG CGGATTTTCT TGGCTGACTC GCAAGGCTTC 
CACCGGTTCA TGGCTTCGGA TCTGGAACAC GAGGACATGG TGGAGACAGC AGTCTTGGGT 
CCTGAGAACA ATCTGATCAT CACAGGGTCC CGGGATGCAC TTATCCAGGT GTGGAGTTTG 
TCAGAACAGG GGACCCTGCT GAATGTCCTT GAGGGCGTGG GCGCCCCTGT GAGTCTGCTG 
GTCCGGGGTG GGACTCTGGT GGTGTCTGCA TCCCGGAAGT CTTCATCTTT CAAGGTCTGG 
GATCTCAAGT CCACTAAGAA GCTGCAGTCT CCGACTCCAT TTCTGGACCG CACCGGCCTC 
GCTGCAGTGT CCCACCATGG CAGCTTTGTC TACTTCCCTA AGGTTGGGGA CAAAAACAAA 
GTCACAATTT GGGACTTGGC AGAAGGTGAG GAGCAAGATT GCCTGGACAC GTCCAATGAG 
GTCCGCTGTC TGGAGGTGGC CGAGCAGGCC AAGCTCCTCT TCACAGGCCT GGTTTCTGGG 
ATCGTGCTTG TGTTCCCCCT GAATTCCAGG CAAGATGTAC TGTGCATCCC CCCACCTGAG 
GCACGCAAAG CTGTCAACTG CATGTCCCTG AGCAAGAGTG AGAATCGTCT GGCCATTGCC 
TATGACAACA TTGTCCTGGT GCTTGACATC AGCCCTGGGG ACCCATGTCC GGCCATTGAA 
GGCCCCACCT ACACCTTTTA CACCCAGCTG CCTGAGACCA TAGTGAGTGT GGCTGTCCTG 
GCTGACTATC GTGTGGTCTA CGGCATGAGC GATGGCAGCC TCTTCCTGTA CGACTGTGCA 
TGCTCTAAAG TGTTCCCTCT GGAGGCCCAT GGGAGCAGGG TGAGCTGTGT AGAGGTCAGC 
CACAGTGAGC AGCTAGCAGT GAGCGGGGCT GAAGATGCAC TGCTATGCCT CTGGGACCTG 
CAGGCTTGCA GGGGGATGTT CGAGATGAGC TATGAGAACT CCTGCTGCAG AGGGGTGCGG 
TGTGCCTGCT TCTCCAGGGA TGACAAGCAT GTATTTGCAG GCATGGAGGA CCGCTCTGTC 
ACTGCCTGGA GCACGGTGGA TGGCACTCTT TTGGCTGTCC AGTTTGTTCA TGCTGTGATC 
AACCGAATCA TCCCTACCTC CAATGGCTTC ATGGCCCCCA CAAGCCACGG CTATCTCATC 
CGTGAGCGCT TCCAGTGCCC GTCAGTCAGA GCCTCCCAGC AAGACCCTCT CAAGAACTTC 
AAGAAGGCAG TGTGGCTGGT AAAGACCAGG CAGAGGGAAG AGCTGGCTGC AGCAGAAGCC 
TCCCAGGATG CAGAACCAGT GGCCGTAGAG GGAAAGGAAT CAAAGTCAAA TAAACGCTCA 
CAGGTCTGCC TCATACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nwd1 ( XM_006531089.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531152.1 Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu41532
Clone ID Related Accession (Same CDS sequence)	XM_006531088.3 , XM_006531089.2
Accession Version	XM_006531089.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006531089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATC GGCTGGTAGA TGGCTGCCTG GATACCAATG CCCAGAGTCT TCTCAGTGGT 
CTTAAAGGGC GCATACTAGA TGCTCAGCCT GGAGCCCTAA AGTCCCACCA CCTGTCCTGG 
AGCCGAGACC TGGTCAACCC CAAGAACAAG GCTCACGCTC GTTACCTAAA GCAGTTGAGC 
GAGCAGTTCG TGGCCAGGAC CAACCATCAG GTCCTGGAGC AGCTTCGAGA GCTGGAGTTG 
GCCAGACAGG AGCTGGGTTG GCTCTACCAA GAGATCCGCC ACCACCTCTG GCAGAGCACA 
GAGTCTACCA AGGTCTTCTG CGGCCACCAG GAGCTCCTGG CCCAGCTCAG GCAGCAGCTC 
AGACAGGATG AGAGCCGGAC ACATACTCCC TTGGTTCTCT TTGGACCACC AGGCATCGGG 
AAGACCTCTC TCATGTGCAA GCTAGCCCAG CAGGTACCAG AGCTACTTGG CCACAAGACA 
GTGGTTGTCC TGAGGCTGCT AGGGACATCA AAGCTGAGCC TGGATGCCCG CAGCCTCCTC 
AGGAGCCTGT CCTTCCAAGT GTGCTTGGCA TATGGGCTGC CACTGCCCCC AGCCCAGGTC 
CTGGAGGCCC ACAGCAGGGT GGGCCATTTC TTCCATACTC TCCTCCACAC CGTGTCACAG 
AGGAACTTTG AGTCCCTGGT GTTGCTCCTG GACTCCGTGG ATGACCTGGA CTCCATATGC 
CATTCTCCGA GGGTCTCTTG GCTGCCCCTC AAGTGTCCTC CAAGGGTGCA CCTCATCCTC 
TCTACATGCT CAGGCCAGCA GGTTTTACAT AACCTGCAAC AGACCCTCAA GGACCCCAGC 
ACCTACTGGG AGGTGAAGGC CCTGTCTGGA AGTCAAGGCC AGGAGTTCAT ACAGCTGCTG 
CTGGCTGCAG AGAAGAGGAT GCTGAGCCCG GGTCAGAGAG ATGTCCTCTG GGCCAGCCTC 
CCAGAGTGTG GCCACCCAGG GAGGCTAAGG CTGGCCTTTG AGGAGGCCCG GAAGTGGGCC 
TCCTTCACTG TGCCAGTGCC CTTGGCCACT ACAGCAGAGG AAGCCACACA CCAGCTCTGT 
ATACGCCTGG AGGAGACACA TGGGGCACTG CTGGTGGCTC ATGTACTGGG CTATATTGTG 
TCTTCCAGGG ATTTGGACAG AACCTGGATT TTGTGGAGGT ACGGTCTCTC AGAAGCAGAG 
CTGAAGGATG TGTTGTCTTT GGATGATGAA GTCTTGCAGG CTGTGTACCG GGACTGGACG 
CCACCTAGCA AAGAGCTGCT GCGCTTCCCG CCCCTGCTGT GGGTGCGGCT CCGCCGGGAC 
CTGGGACACT GCCTAGTCAG GAGGCCCGTG GATGGCTGCA TGCTACTGGC CATTGCACAC 
AGACAGCTGT CCCAGGTAAT CCAAGTTCGA TATCTGTCTG GGCCTGAGAG AGCCAAGAGG 
CATGGCGTGC TAGCTGAATT CTTCTCTGGA GCCTGGAGCC AGGGGATCAA GAAACTTATC 
ACCCTGCCGC TTGTAGGGAA GCCCCTGAAC CTGGACCGCA AGGTGGCCCC ACAGCCACTG 
TGGTTCTCAA GCACAGTGGC CAACCTGAGG AAGCTGACCG AACTGCCCTT CCATCTGCTG 
CATGCAGGCC GCTTGGAGGA GTTGAAGCAG GAGGTTCTGG GGAACATGAG TTGGATCTCA 
TGTCGGGGCA TCTCTGGAGG CATTGAGGTC CTGCTGGATG ATTTTGACAT GTGTGCCCCT 
CACATGAACT CTCCTGAGGT TGATCTGGTT AGAGAAGCCA TCCAGCTGTG TGGCCCTGCT 
GTGGAGCTCC GGGGCCTGGA GAAGAGCGTC CTATACACCG AGCTCCTGGC CAGACTCCTT 
TTCTTTGCTG CATCACATCC AGCAATGATC GGACAACTGT GCCAACAAGC CCAGAGCTGG 
TTCCGTGCCT GCCCCTACCC AATGCTGGTG CCCCTGGCAG GATTCCTGCA GCCACCTGGT 
GGACCTCTTC GAGCCACTCT TACTGGGTGT CACAAAGGCA TCACCGCCAT AGCATGGAGC 
CTGGAGGAGA AACTCCTGGT GGTGGGCACC CAGGATGGTG CCATGGTCGT GTGGGACGTG 
GAGGAACAAC AGGTGGTACA CGTTCTAATG GGACACACAG CTGAAGTGAA GTGTGTGCGA 
GTGTTTGCCC AAGGGACTCT TGCCATCTCT GCATCAAAGG ACCATACCCT GCGTCTGTGG 
AGTCTGCTTT CCGGCCAGGA GAAAGTCACC ATTTTGGATG GAGGGTCACA AAATCCCACA 
GAGCCCCAGA GTTGGGACCT TCATGTGGAT GAGAGAAACA ATGTTGTATA CTCGACATCT 
GGTGCAAGGA TCAACATGTG GAATCTGGAA ACTTCGAAGC TGGTTTTCTG TATCACAGGA 
GATGTGTCTG ATCCCTGGGT CTGTGTGGCT TTGTTGGCTG CCCAGGGTTT GCTGCTTGCC 
CTGTCCAAGG GTGGCCAGGT CAGTCTGTGG AGCTCAGCCA TGGGAAAACT GCAGGAGAAA 
CACCAGTTAT CCAGCATCAA AGAAGAGACA CCCACCTGTG CCGTCTCCAT CCAGTCCCGG 
GCAAGGCTGG TCGCTGGCTT CAGCAGTGGC TCCATTGCCC TGGTCTCCGC TGGAGAAGAC 
AGGCTGCTGG AGAAGCTCCC AGAAGCTGTG GGGTTCCTGG TGGTTTCTGA AGACGATTCC 
CTTCTTGTGG CAGGCTTTGG AAGATTTGTG CGGATTTTCT TGGCTGACTC GCAAGGCTTC 
CACCGGTTCA TGGCTTCGGA TCTGGAACAC GAGGACATGG TGGAGACAGC AGTCTTGGGT 
CCTGAGAACA ATCTGATCAT CACAGGGTCC CGGGATGCAC TTATCCAGGT GTGGAGTTTG 
TCAGAACAGG GGACCCTGCT GAATGTCCTT GAGGGCGTGG GCGCCCCTGT GAGTCTGCTG 
GTCCGGGGTG GGACTCTGGT GGTGTCTGCA TCCCGGAAGT CTTCATCTTT CAAGGTCTGG 
GATCTCAAGT CCACTAAGAA GCTGCAGTCT CCGACTCCAT TTCTGGACCG CACCGGCCTC 
GCTGCAGTGT CCCACCATGG CAGCTTTGTC TACTTCCCTA AGGTTGGGGA CAAAAACAAA 
GTCACAATTT GGGACTTGGC AGAAGGTGAG GAGCAAGATT GCCTGGACAC GTCCAATGAG 
GTCCGCTGTC TGGAGGTGGC CGAGCAGGCC AAGCTCCTCT TCACAGGCCT GGTTTCTGGG 
ATCGTGCTTG TGTTCCCCCT GAATTCCAGG CAAGATGTAC TGTGCATCCC CCCACCTGAG 
GCACGCAAAG CTGTCAACTG CATGTCCCTG AGCAAGAGTG AGAATCGTCT GGCCATTGCC 
TATGACAACA TTGTCCTGGT GCTTGACATC AGCCCTGGGG ACCCATGTCC GGCCATTGAA 
GGCCCCACCT ACACCTTTTA CACCCAGCTG CCTGAGACCA TAGTGAGTGT GGCTGTCCTG 
GCTGACTATC GTGTGGTCTA CGGCATGAGC GATGGCAGCC TCTTCCTGTA CGACTGTGCA 
TGCTCTAAAG TGTTCCCTCT GGAGGCCCAT GGGAGCAGGG TGAGCTGTGT AGAGGTCAGC 
CACAGTGAGC AGCTAGCAGT GAGCGGGGCT GAAGATGCAC TGCTATGCCT CTGGGACCTG 
CAGGCTTGCA GGGGGATGTT CGAGATGAGC TATGAGAACT CCTGCTGCAG AGGGGTGCGG 
TGTGCCTGCT TCTCCAGGGA TGACAAGCAT GTATTTGCAG GCATGGAGGA CCGCTCTGTC 
ACTGCCTGGA GCACGGTGGA TGGCACTCTT TTGGCTGTCC AGTTTGTTCA TGCTGTGATC 
AACCGAATCA TCCCTACCTC CAATGGCTTC ATGGCCCCCA CAAGCCACGG CTATCTCATC 
CGTGAGCGCT TCCAGTGCCC GTCAGTCAGA GCCTCCCAGC AAGACCCTCT CAAGAACTTC 
AAGAAGGCAG TGTGGCTGGT AAAGACCAGG CAGAGGGAAG AGCTGGCTGC AGCAGAAGCC 
TCCCAGGATG CAGAACCAGT GGCCGTAGAG GGAAAGGAAT CAAAGTCAAA TAAACGCTCA 
CAGGTCTGCC TCATACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531152.1
CDS	213..4313
Translation	MVDRLVDGCLDTNAQSLLSGLKGRILDAQPGALKSHHLSWSRDLVNPKNKAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPGIGKTSLMCKLAQQVPELLGHKTVVVLRLLGTSKLSLDARSLLRSLSFQVCLAYGLPLPPAQVLEAHSRVGHFFHTLLHTVSQRNFESLVLLLDSVDDLDSICHSPRVSWLPLKCPPRVHLILSTCSGQQVLHNLQQTLKDPSTYWEVKALSGSQGQEFIQLLLAAEKRMLSPGQRDVLWASLPECGHPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCIRLEETHGALLVAHVLGYIVSSRDLDRTWILWRYGLSEAELKDVLSLDDEVLQAVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLVRRPVDGCMLLAIAHRQLSQVIQVRYLSGPERAKRHGVLAEFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSSTVANLRKLTELPFHLLHAGRLEELKQEVLGNMSWISCRGISGGIEVLLDDFDMCAPHMNSPEVDLVREAIQLCGPAVELRGLEKSVLYTELLARLLFFAASHPAMIGQLCQQAQSWFRACPYPMLVPLAGFLQPPGGPLRATLTGCHKGITAIAWSLEEKLLVVGTQDGAMVVWDVEEQQVVHVLMGHTAEVKCVRVFAQGTLAISASKDHTLRLWSLLSGQEKVTILDGGSQNPTEPQSWDLHVDERNNVVYSTSGARINMWNLETSKLVFCITGDVSDPWVCVALLAAQGLLLALSKGGQVSLWSSAMGKLQEKHQLSSIKEETPTCAVSIQSRARLVAGFSSGSIALVSAGEDRLLEKLPEAVGFLVVSEDDSLLVAGFGRFVRIFLADSQGFHRFMASDLEHEDMVETAVLGPENNLIITGSRDALIQVWSLSEQGTLLNVLEGVGAPVSLLVRGGTLVVSASRKSSSFKVWDLKSTKKLQSPTPFLDRTGLAAVSHHGSFVYFPKVGDKNKVTIWDLAEGEEQDCLDTSNEVRCLEVAEQAKLLFTGLVSGIVLVFPLNSRQDVLCIPPPEARKAVNCMSLSKSENRLAIAYDNIVLVLDISPGDPCPAIEGPTYTFYTQLPETIVSVAVLADYRVVYGMSDGSLFLYDCACSKVFPLEAHGSRVSCVEVSHSEQLAVSGAEDALLCLWDLQACRGMFEMSYENSCCRGVRCACFSRDDKHVFAGMEDRSVTAWSTVDGTLLAVQFVHAVINRIIPTSNGFMAPTSHGYLIRERFQCPSVRASQQDPLKNFKKAVWLVKTRQREELAAAEASQDAEPVAVEGKESKSNKRSQVCLIL

Target ORF information:

RefSeq Version	XM_006531089.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531089.2

ORF Insert Sequence:

ATGGTGGATC GGCTGGTAGA TGGCTGCCTG GATACCAATG CCCAGAGTCT TCTCAGTGGT 
CTTAAAGGGC GCATACTAGA TGCTCAGCCT GGAGCCCTAA AGTCCCACCA CCTGTCCTGG 
AGCCGAGACC TGGTCAACCC CAAGAACAAG GCTCACGCTC GTTACCTAAA GCAGTTGAGC 
GAGCAGTTCG TGGCCAGGAC CAACCATCAG GTCCTGGAGC AGCTTCGAGA GCTGGAGTTG 
GCCAGACAGG AGCTGGGTTG GCTCTACCAA GAGATCCGCC ACCACCTCTG GCAGAGCACA 
GAGTCTACCA AGGTCTTCTG CGGCCACCAG GAGCTCCTGG CCCAGCTCAG GCAGCAGCTC 
AGACAGGATG AGAGCCGGAC ACATACTCCC TTGGTTCTCT TTGGACCACC AGGCATCGGG 
AAGACCTCTC TCATGTGCAA GCTAGCCCAG CAGGTACCAG AGCTACTTGG CCACAAGACA 
GTGGTTGTCC TGAGGCTGCT AGGGACATCA AAGCTGAGCC TGGATGCCCG CAGCCTCCTC 
AGGAGCCTGT CCTTCCAAGT GTGCTTGGCA TATGGGCTGC CACTGCCCCC AGCCCAGGTC 
CTGGAGGCCC ACAGCAGGGT GGGCCATTTC TTCCATACTC TCCTCCACAC CGTGTCACAG 
AGGAACTTTG AGTCCCTGGT GTTGCTCCTG GACTCCGTGG ATGACCTGGA CTCCATATGC 
CATTCTCCGA GGGTCTCTTG GCTGCCCCTC AAGTGTCCTC CAAGGGTGCA CCTCATCCTC 
TCTACATGCT CAGGCCAGCA GGTTTTACAT AACCTGCAAC AGACCCTCAA GGACCCCAGC 
ACCTACTGGG AGGTGAAGGC CCTGTCTGGA AGTCAAGGCC AGGAGTTCAT ACAGCTGCTG 
CTGGCTGCAG AGAAGAGGAT GCTGAGCCCG GGTCAGAGAG ATGTCCTCTG GGCCAGCCTC 
CCAGAGTGTG GCCACCCAGG GAGGCTAAGG CTGGCCTTTG AGGAGGCCCG GAAGTGGGCC 
TCCTTCACTG TGCCAGTGCC CTTGGCCACT ACAGCAGAGG AAGCCACACA CCAGCTCTGT 
ATACGCCTGG AGGAGACACA TGGGGCACTG CTGGTGGCTC ATGTACTGGG CTATATTGTG 
TCTTCCAGGG ATTTGGACAG AACCTGGATT TTGTGGAGGT ACGGTCTCTC AGAAGCAGAG 
CTGAAGGATG TGTTGTCTTT GGATGATGAA GTCTTGCAGG CTGTGTACCG GGACTGGACG 
CCACCTAGCA AAGAGCTGCT GCGCTTCCCG CCCCTGCTGT GGGTGCGGCT CCGCCGGGAC 
CTGGGACACT GCCTAGTCAG GAGGCCCGTG GATGGCTGCA TGCTACTGGC CATTGCACAC 
AGACAGCTGT CCCAGGTAAT CCAAGTTCGA TATCTGTCTG GGCCTGAGAG AGCCAAGAGG 
CATGGCGTGC TAGCTGAATT CTTCTCTGGA GCCTGGAGCC AGGGGATCAA GAAACTTATC 
ACCCTGCCGC TTGTAGGGAA GCCCCTGAAC CTGGACCGCA AGGTGGCCCC ACAGCCACTG 
TGGTTCTCAA GCACAGTGGC CAACCTGAGG AAGCTGACCG AACTGCCCTT CCATCTGCTG 
CATGCAGGCC GCTTGGAGGA GTTGAAGCAG GAGGTTCTGG GGAACATGAG TTGGATCTCA 
TGTCGGGGCA TCTCTGGAGG CATTGAGGTC CTGCTGGATG ATTTTGACAT GTGTGCCCCT 
CACATGAACT CTCCTGAGGT TGATCTGGTT AGAGAAGCCA TCCAGCTGTG TGGCCCTGCT 
GTGGAGCTCC GGGGCCTGGA GAAGAGCGTC CTATACACCG AGCTCCTGGC CAGACTCCTT 
TTCTTTGCTG CATCACATCC AGCAATGATC GGACAACTGT GCCAACAAGC CCAGAGCTGG 
TTCCGTGCCT GCCCCTACCC AATGCTGGTG CCCCTGGCAG GATTCCTGCA GCCACCTGGT 
GGACCTCTTC GAGCCACTCT TACTGGGTGT CACAAAGGCA TCACCGCCAT AGCATGGAGC 
CTGGAGGAGA AACTCCTGGT GGTGGGCACC CAGGATGGTG CCATGGTCGT GTGGGACGTG 
GAGGAACAAC AGGTGGTACA CGTTCTAATG GGACACACAG CTGAAGTGAA GTGTGTGCGA 
GTGTTTGCCC AAGGGACTCT TGCCATCTCT GCATCAAAGG ACCATACCCT GCGTCTGTGG 
AGTCTGCTTT CCGGCCAGGA GAAAGTCACC ATTTTGGATG GAGGGTCACA AAATCCCACA 
GAGCCCCAGA GTTGGGACCT TCATGTGGAT GAGAGAAACA ATGTTGTATA CTCGACATCT 
GGTGCAAGGA TCAACATGTG GAATCTGGAA ACTTCGAAGC TGGTTTTCTG TATCACAGGA 
GATGTGTCTG ATCCCTGGGT CTGTGTGGCT TTGTTGGCTG CCCAGGGTTT GCTGCTTGCC 
CTGTCCAAGG GTGGCCAGGT CAGTCTGTGG AGCTCAGCCA TGGGAAAACT GCAGGAGAAA 
CACCAGTTAT CCAGCATCAA AGAAGAGACA CCCACCTGTG CCGTCTCCAT CCAGTCCCGG 
GCAAGGCTGG TCGCTGGCTT CAGCAGTGGC TCCATTGCCC TGGTCTCCGC TGGAGAAGAC 
AGGCTGCTGG AGAAGCTCCC AGAAGCTGTG GGGTTCCTGG TGGTTTCTGA AGACGATTCC 
CTTCTTGTGG CAGGCTTTGG AAGATTTGTG CGGATTTTCT TGGCTGACTC GCAAGGCTTC 
CACCGGTTCA TGGCTTCGGA TCTGGAACAC GAGGACATGG TGGAGACAGC AGTCTTGGGT 
CCTGAGAACA ATCTGATCAT CACAGGGTCC CGGGATGCAC TTATCCAGGT GTGGAGTTTG 
TCAGAACAGG GGACCCTGCT GAATGTCCTT GAGGGCGTGG GCGCCCCTGT GAGTCTGCTG 
GTCCGGGGTG GGACTCTGGT GGTGTCTGCA TCCCGGAAGT CTTCATCTTT CAAGGTCTGG 
GATCTCAAGT CCACTAAGAA GCTGCAGTCT CCGACTCCAT TTCTGGACCG CACCGGCCTC 
GCTGCAGTGT CCCACCATGG CAGCTTTGTC TACTTCCCTA AGGTTGGGGA CAAAAACAAA 
GTCACAATTT GGGACTTGGC AGAAGGTGAG GAGCAAGATT GCCTGGACAC GTCCAATGAG 
GTCCGCTGTC TGGAGGTGGC CGAGCAGGCC AAGCTCCTCT TCACAGGCCT GGTTTCTGGG 
ATCGTGCTTG TGTTCCCCCT GAATTCCAGG CAAGATGTAC TGTGCATCCC CCCACCTGAG 
GCACGCAAAG CTGTCAACTG CATGTCCCTG AGCAAGAGTG AGAATCGTCT GGCCATTGCC 
TATGACAACA TTGTCCTGGT GCTTGACATC AGCCCTGGGG ACCCATGTCC GGCCATTGAA 
GGCCCCACCT ACACCTTTTA CACCCAGCTG CCTGAGACCA TAGTGAGTGT GGCTGTCCTG 
GCTGACTATC GTGTGGTCTA CGGCATGAGC GATGGCAGCC TCTTCCTGTA CGACTGTGCA 
TGCTCTAAAG TGTTCCCTCT GGAGGCCCAT GGGAGCAGGG TGAGCTGTGT AGAGGTCAGC 
CACAGTGAGC AGCTAGCAGT GAGCGGGGCT GAAGATGCAC TGCTATGCCT CTGGGACCTG 
CAGGCTTGCA GGGGGATGTT CGAGATGAGC TATGAGAACT CCTGCTGCAG AGGGGTGCGG 
TGTGCCTGCT TCTCCAGGGA TGACAAGCAT GTATTTGCAG GCATGGAGGA CCGCTCTGTC 
ACTGCCTGGA GCACGGTGGA TGGCACTCTT TTGGCTGTCC AGTTTGTTCA TGCTGTGATC 
AACCGAATCA TCCCTACCTC CAATGGCTTC ATGGCCCCCA CAAGCCACGG CTATCTCATC 
CGTGAGCGCT TCCAGTGCCC GTCAGTCAGA GCCTCCCAGC AAGACCCTCT CAAGAACTTC 
AAGAAGGCAG TGTGGCTGGT AAAGACCAGG CAGAGGGAAG AGCTGGCTGC AGCAGAAGCC 
TCCCAGGATG CAGAACCAGT GGCCGTAGAG GGAAAGGAAT CAAAGTCAAA TAAACGCTCA 
CAGGTCTGCC TCATACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nwd1 ( XM_006531090.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531153.1 Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu41533
Clone ID Related Accession (Same CDS sequence)	XM_006531090.2
Accession Version	XM_006531090.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006531090.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAG AGCGAGAAGC CCTGCAGTGC ACAGCCTACC CTGAGGTTCA GAGCTTCTGC 
CAAAGGCACG GCTTGGCGTT TGAGGTGGTT GATCTGAGGT GGGGTATTCC GAATACGCAG 
GCCACTGACT ACCTGACCAC GGAACTCTGC TTAGAAGAAC TGGAGCGCTG TCAGAAAACA 
TCCATAGGAC CAGCTTTTGT GGCCCTCCTG GGTGACCAGT ACGGCCCCTG CCCGGTTCCC 
CGGAGGATTG AGGAAAAGGA GTGGGAGGCA CTAAGAGCTC AGCTGACCTC CAGGCCTCGA 
GATCTGGAGC TGGTGACCAG ACATTTCCAG AGAGACGACA ACACCATTCC TCCAACCTAC 
GTCTTGCAGC CTTCAGGCTC ACTGGTGGTC CCAGGGCCTG AGGAGGCCAC CTTAACCTCT 
GTCCTGAGAG GTGGAGCCCA GGAGGCCTGG AGGCTAGGCC TCATCAGCCA GGAGCAATGG 
ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG GATTTGGACA GAACCTGGAT TTTGTGGAGG 
TACGGTCTCT CAGAAGCAGA GCTGAAGGAT GTGTTGTCTT TGGATGATGA AGTCTTGCAG 
GCTGTGTACC GGGACTGGAC GCCACCTAGC AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG 
TGGGTGCGGC TCCGCCGGGA CCTGGGACAC TGCCTAGTCA GGAGGCCCGT GGATGGCTGC 
ATGCTACTGG CCATTGCACA CAGACAGCTG TCCCAGGTAA TCCAAGTTCG ATATCTGTCT 
GGGCCTGAGA GAGCCAAGAG GCATGGCGTG CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC 
CAGGGGATCA AGAAACTTAT CACCCTGCCG CTTGTAGGGA AGCCCCTGAA CCTGGACCGC 
AAGGTGGCCC CACAGCCACT GTGGTTCTCA AGCACAGTGG CCAACCTGAG GAAGCTGACC 
GAACTGCCCT TCCATCTGCT GCATGCAGGC CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG 
GGGAACATGA GTTGGATCTC ATGTCGGGGC ATCTCTGGAG GCATTGAGGT CCTGCTGGAT 
GATTTTGACA TGTGTGCCCC TCACATGAAC TCTCCTGAGG TTGATCTGGT TAGAGAAGCC 
ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC CGGGGCCTGG AGAAGAGCGT CCTATACACC 
GAGCTCCTGG CCAGACTCCT TTTCTTTGCT GCATCACATC CAGCAATGAT CGGACAACTG 
TGCCAACAAG CCCAGAGCTG GTTCCGTGCC TGCCCCTACC CAATGCTGGT GCCCCTGGCA 
GGATTCCTGC AGCCACCTGG TGGACCTCTT CGAGCCACTC TTACTGGGTG TCACAAAGGC 
ATCACCGCCA TAGCATGGAG CCTGGAGGAG AAACTCCTGG TGGTGGGCAC CCAGGATGGT 
GCCATGGTCG TGTGGGACGT GGAGGAACAA CAGGTGGTAC ACGTTCTAAT GGGACACACA 
GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC CAAGGGACTC TTGCCATCTC TGCATCAAAG 
GACCATACCC TGCGTCTGTG GAGTCTGCTT TCCGGCCAGG AGAAAGTCAC CATTTTGGAT 
GGAGGGTCAC AAAATCCCAC AGAGCCCCAG AGTTGGGACC TTCATGTGGA TGAGAGAAAC 
AATGTTGTAT ACTCGACATC TGGTGCAAGG ATCAACATGT GGAATCTGGA AACTTCGAAG 
CTGGTTTTCT GTATCACAGG AGATGTGTCT GATCCCTGGG TCTGTGTGGC TTTGTTGGCT 
GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC 
ATGGGAAAAC TGCAGGAGAA ACACCAGTTA TCCAGCATCA AAGAAGAGAC ACCCACCTGT 
GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG GTCGCTGGCT TCAGCAGTGG CTCCATTGCC 
CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG 
GTGGTTTCTG AAGACGATTC CCTTCTTGTG GCAGGCTTTG GAAGATTTGT GCGGATTTTC 
TTGGCTGACT CGCAAGGCTT CCACCGGTTC ATGGCTTCGG ATCTGGAACA CGAGGACATG 
GTGGAGACAG CAGTCTTGGG TCCTGAGAAC AATCTGATCA TCACAGGGTC CCGGGATGCA 
CTTATCCAGG TGTGGAGTTT GTCAGAACAG GGGACCCTGC TGAATGTCCT TGAGGGCGTG 
GGCGCCCCTG TGAGTCTGCT GGTCCGGGGT GGGACTCTGG TGGTGTCTGC ATCCCGGAAG 
TCTTCATCTT TCAAGGTCTG GGATCTCAAG TCCACTAAGA AGCTGCAGTC TCCGACTCCA 
TTTCTGGACC GCACCGGCCT CGCTGCAGTG TCCCACCATG GCAGCTTTGT CTACTTCCCT 
AAGGTTGGGG ACAAAAACAA AGTCACAATT TGGGACTTGG CAGAAGGTGA GGAGCAAGAT 
TGCCTGGACA CGTCCAATGA GGTCCGCTGT CTGGAGGTGG CCGAGCAGGC CAAGCTCCTC 
TTCACAGGCC TGGTTTCTGG GATCGTGCTT GTGTTCCCCC TGAATTCCAG GCAAGATGTA 
CTGTGCATCC CCCCACCTGA GGCACGCAAA GCTGTCAACT GCATGTCCCT GAGCAAGAGT 
GAGAATCGTC TGGCCATTGC CTATGACAAC ATTGTCCTGG TGCTTGACAT CAGCCCTGGG 
GACCCATGTC CGGCCATTGA AGGCCCCACC TACACCTTTT ACACCCAGCT GCCTGAGACC 
ATAAACTCCT GCTGCAGAGG GGTGCGGTGT GCCTGCTTCT CCAGGGATGA CAAGCATGTA 
TTTGCAGGCA TGGAGGACCG CTCTGTCACT GCCTGGAGCA CGGTGGATGG CACTCTTTTG 
GCTGTCCAGT TTGTTCATGC TGTGATCAAC CGAATCATCC CTACCTCCAA TGGCTTCATG 
GCCCCCACAA GCCACGGCTA TCTCATCCGT GAGCGCTTCC AGTGCCCGTC AGTCAGAGCC 
TCCCAGCAAG ACCCTCTCAA GAACTTCAAG AAGGCAGTGT GGCTGGTAAA GACCAGGCAG 
AGGGAAGAGC TGGCTGCAGC AGAAGCCTCC CAGGATGCAG AACCAGTGGC CGTAGAGGGA 
AAGGAATCAA AGTCAAATAA ACGCTCACAG GTCTGCCTCA TACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531153.1
CDS	786..5273
Translation	MDTEREALQCTAYPEVQSFCQRHGLAFEVVDLRWGIPNTQATDYLTTELCLEELERCQKTSIGPAFVALLGDQYGPCPVPRRIEEKEWEALRAQLTSRPRDLELVTRHFQRDDNTIPPTYVLQPSGSLVVPGPEEATLTSVLRGGAQEAWRLGLISQEQWMCYHRSVIEWEIELGLLSSARGDQGATVFLRDVQDLNKHILDDCSLKMVDRLVDGCLDTNAQSLLSGLKGRILDAQPGALKSHHLSWSRDLVNPKNKAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPGIGKTSLMCKLAQQVPELLGHKTVVVLRLLGTSKLSLDARSLLRSLSFQVCLAYGLPLPPAQVLEAHSRVGHFFHTLLHTVSQRNFESLVLLLDSVDDLDSICHSPRVSWLPLKCPPRVHLILSTCSGQQVLHNLQQTLKDPSTYWEVKALSGSQGQEFIQLLLAAEKRMLSPGQRDVLWASLPECGHPGRLRLAFEEARKWASFTVPVPLATTAEEATHQLCIRLEETHGALLVAHVLGYIVSSRDLDRTWILWRYGLSEAELKDVLSLDDEVLQAVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLVRRPVDGCMLLAIAHRQLSQVIQVRYLSGPERAKRHGVLAEFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSSTVANLRKLTELPFHLLHAGRLEELKQEVLGNMSWISCRGISGGIEVLLDDFDMCAPHMNSPEVDLVREAIQLCGPAVELRGLEKSVLYTELLARLLFFAASHPAMIGQLCQQAQSWFRACPYPMLVPLAGFLQPPGGPLRATLTGCHKGITAIAWSLEEKLLVVGTQDGAMVVWDVEEQQVVHVLMGHTAEVKCVRVFAQGTLAISASKDHTLRLWSLLSGQEKVTILDGGSQNPTEPQSWDLHVDERNNVVYSTSGARINMWNLETSKLVFCITGDVSDPWVCVALLAAQGLLLALSKGGQVSLWSSAMGKLQEKHQLSSIKEETPTCAVSIQSRARLVAGFSSGSIALVSAGEDRLLEKLPEAVGFLVVSEDDSLLVAGFGRFVRIFLADSQGFHRFMASDLEHEDMVETAVLGPENNLIITGSRDALIQVWSLSEQGTLLNVLEGVGAPVSLLVRGGTLVVSASRKSSSFKVWDLKSTKKLQSPTPFLDRTGLAAVSHHGSFVYFPKVGDKNKVTIWDLAEGEEQDCLDTSNEVRCLEVAEQAKLLFTGLVSGIVLVFPLNSRQDVLCIPPPEARKAVNCMSLSKSENRLAIAYDNIVLVLDISPGDPCPAIEGPTYTFYTQLPETINSCCRGVRCACFSRDDKHVFAGMEDRSVTAWSTVDGTLLAVQFVHAVINRIIPTSNGFMAPTSHGYLIRERFQCPSVRASQQDPLKNFKKAVWLVKTRQREELAAAEASQDAEPVAVEGKESKSNKRSQVCLIL

Target ORF information:

RefSeq Version	XM_006531090.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NACHT and WD repeat domain containing 1 (Nwd1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531090.2

ORF Insert Sequence:

ATGGACACAG AGCGAGAAGC CCTGCAGTGC ACAGCCTACC CTGAGGTTCA GAGCTTCTGC 
CAAAGGCACG GCTTGGCGTT TGAGGTGGTT GATCTGAGGT GGGGTATTCC GAATACGCAG 
GCCACTGACT ACCTGACCAC GGAACTCTGC TTAGAAGAAC TGGAGCGCTG TCAGAAAACA 
TCCATAGGAC CAGCTTTTGT GGCCCTCCTG GGTGACCAGT ACGGCCCCTG CCCGGTTCCC 
CGGAGGATTG AGGAAAAGGA GTGGGAGGCA CTAAGAGCTC AGCTGACCTC CAGGCCTCGA 
GATCTGGAGC TGGTGACCAG ACATTTCCAG AGAGACGACA ACACCATTCC TCCAACCTAC 
GTCTTGCAGC CTTCAGGCTC ACTGGTGGTC CCAGGGCCTG AGGAGGCCAC CTTAACCTCT 
GTCCTGAGAG GTGGAGCCCA GGAGGCCTGG AGGCTAGGCC TCATCAGCCA GGAGCAATGG 
ATGTGCTATC ACCGATCAGT TATTGAGTGG GAGATCGAGC TTGGCCTACT GAGCTCAGCA 
AGAGGGGACC AGGGAGCTAC CGTCTTCCTG AGAGATGTAC AGGACCTCAA TAAACACATC 
TTGGATGATT GCTCTCTGAA GATGGTGGAT CGGCTGGTAG ATGGCTGCCT GGATACCAAT 
GCCCAGAGTC TTCTCAGTGG TCTTAAAGGG CGCATACTAG ATGCTCAGCC TGGAGCCCTA 
AAGTCCCACC ACCTGTCCTG GAGCCGAGAC CTGGTCAACC CCAAGAACAA GGCTCACGCT 
CGTTACCTAA AGCAGTTGAG CGAGCAGTTC GTGGCCAGGA CCAACCATCA GGTCCTGGAG 
CAGCTTCGAG AGCTGGAGTT GGCCAGACAG GAGCTGGGTT GGCTCTACCA AGAGATCCGC 
CACCACCTCT GGCAGAGCAC AGAGTCTACC AAGGTCTTCT GCGGCCACCA GGAGCTCCTG 
GCCCAGCTCA GGCAGCAGCT CAGACAGGAT GAGAGCCGGA CACATACTCC CTTGGTTCTC 
TTTGGACCAC CAGGCATCGG GAAGACCTCT CTCATGTGCA AGCTAGCCCA GCAGGTACCA 
GAGCTACTTG GCCACAAGAC AGTGGTTGTC CTGAGGCTGC TAGGGACATC AAAGCTGAGC 
CTGGATGCCC GCAGCCTCCT CAGGAGCCTG TCCTTCCAAG TGTGCTTGGC ATATGGGCTG 
CCACTGCCCC CAGCCCAGGT CCTGGAGGCC CACAGCAGGG TGGGCCATTT CTTCCATACT 
CTCCTCCACA CCGTGTCACA GAGGAACTTT GAGTCCCTGG TGTTGCTCCT GGACTCCGTG 
GATGACCTGG ACTCCATATG CCATTCTCCG AGGGTCTCTT GGCTGCCCCT CAAGTGTCCT 
CCAAGGGTGC ACCTCATCCT CTCTACATGC TCAGGCCAGC AGGTTTTACA TAACCTGCAA 
CAGACCCTCA AGGACCCCAG CACCTACTGG GAGGTGAAGG CCCTGTCTGG AAGTCAAGGC 
CAGGAGTTCA TACAGCTGCT GCTGGCTGCA GAGAAGAGGA TGCTGAGCCC GGGTCAGAGA 
GATGTCCTCT GGGCCAGCCT CCCAGAGTGT GGCCACCCAG GGAGGCTAAG GCTGGCCTTT 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGTGC CCTTGGCCAC TACAGCAGAG 
GAAGCCACAC ACCAGCTCTG TATACGCCTG GAGGAGACAC ATGGGGCACT GCTGGTGGCT 
CATGTACTGG GCTATATTGT GTCTTCCAGG GATTTGGACA GAACCTGGAT TTTGTGGAGG 
TACGGTCTCT CAGAAGCAGA GCTGAAGGAT GTGTTGTCTT TGGATGATGA AGTCTTGCAG 
GCTGTGTACC GGGACTGGAC GCCACCTAGC AAAGAGCTGC TGCGCTTCCC GCCCCTGCTG 
TGGGTGCGGC TCCGCCGGGA CCTGGGACAC TGCCTAGTCA GGAGGCCCGT GGATGGCTGC 
ATGCTACTGG CCATTGCACA CAGACAGCTG TCCCAGGTAA TCCAAGTTCG ATATCTGTCT 
GGGCCTGAGA GAGCCAAGAG GCATGGCGTG CTAGCTGAAT TCTTCTCTGG AGCCTGGAGC 
CAGGGGATCA AGAAACTTAT CACCCTGCCG CTTGTAGGGA AGCCCCTGAA CCTGGACCGC 
AAGGTGGCCC CACAGCCACT GTGGTTCTCA AGCACAGTGG CCAACCTGAG GAAGCTGACC 
GAACTGCCCT TCCATCTGCT GCATGCAGGC CGCTTGGAGG AGTTGAAGCA GGAGGTTCTG 
GGGAACATGA GTTGGATCTC ATGTCGGGGC ATCTCTGGAG GCATTGAGGT CCTGCTGGAT 
GATTTTGACA TGTGTGCCCC TCACATGAAC TCTCCTGAGG TTGATCTGGT TAGAGAAGCC 
ATCCAGCTGT GTGGCCCTGC TGTGGAGCTC CGGGGCCTGG AGAAGAGCGT CCTATACACC 
GAGCTCCTGG CCAGACTCCT TTTCTTTGCT GCATCACATC CAGCAATGAT CGGACAACTG 
TGCCAACAAG CCCAGAGCTG GTTCCGTGCC TGCCCCTACC CAATGCTGGT GCCCCTGGCA 
GGATTCCTGC AGCCACCTGG TGGACCTCTT CGAGCCACTC TTACTGGGTG TCACAAAGGC 
ATCACCGCCA TAGCATGGAG CCTGGAGGAG AAACTCCTGG TGGTGGGCAC CCAGGATGGT 
GCCATGGTCG TGTGGGACGT GGAGGAACAA CAGGTGGTAC ACGTTCTAAT GGGACACACA 
GCTGAAGTGA AGTGTGTGCG AGTGTTTGCC CAAGGGACTC TTGCCATCTC TGCATCAAAG 
GACCATACCC TGCGTCTGTG GAGTCTGCTT TCCGGCCAGG AGAAAGTCAC CATTTTGGAT 
GGAGGGTCAC AAAATCCCAC AGAGCCCCAG AGTTGGGACC TTCATGTGGA TGAGAGAAAC 
AATGTTGTAT ACTCGACATC TGGTGCAAGG ATCAACATGT GGAATCTGGA AACTTCGAAG 
CTGGTTTTCT GTATCACAGG AGATGTGTCT GATCCCTGGG TCTGTGTGGC TTTGTTGGCT 
GCCCAGGGTT TGCTGCTTGC CCTGTCCAAG GGTGGCCAGG TCAGTCTGTG GAGCTCAGCC 
ATGGGAAAAC TGCAGGAGAA ACACCAGTTA TCCAGCATCA AAGAAGAGAC ACCCACCTGT 
GCCGTCTCCA TCCAGTCCCG GGCAAGGCTG GTCGCTGGCT TCAGCAGTGG CTCCATTGCC 
CTGGTCTCCG CTGGAGAAGA CAGGCTGCTG GAGAAGCTCC CAGAAGCTGT GGGGTTCCTG 
GTGGTTTCTG AAGACGATTC CCTTCTTGTG GCAGGCTTTG GAAGATTTGT GCGGATTTTC 
TTGGCTGACT CGCAAGGCTT CCACCGGTTC ATGGCTTCGG ATCTGGAACA CGAGGACATG 
GTGGAGACAG CAGTCTTGGG TCCTGAGAAC AATCTGATCA TCACAGGGTC CCGGGATGCA 
CTTATCCAGG TGTGGAGTTT GTCAGAACAG GGGACCCTGC TGAATGTCCT TGAGGGCGTG 
GGCGCCCCTG TGAGTCTGCT GGTCCGGGGT GGGACTCTGG TGGTGTCTGC ATCCCGGAAG 
TCTTCATCTT TCAAGGTCTG GGATCTCAAG TCCACTAAGA AGCTGCAGTC TCCGACTCCA 
TTTCTGGACC GCACCGGCCT CGCTGCAGTG TCCCACCATG GCAGCTTTGT CTACTTCCCT 
AAGGTTGGGG ACAAAAACAA AGTCACAATT TGGGACTTGG CAGAAGGTGA GGAGCAAGAT 
TGCCTGGACA CGTCCAATGA GGTCCGCTGT CTGGAGGTGG CCGAGCAGGC CAAGCTCCTC 
TTCACAGGCC TGGTTTCTGG GATCGTGCTT GTGTTCCCCC TGAATTCCAG GCAAGATGTA 
CTGTGCATCC CCCCACCTGA GGCACGCAAA GCTGTCAACT GCATGTCCCT GAGCAAGAGT 
GAGAATCGTC TGGCCATTGC CTATGACAAC ATTGTCCTGG TGCTTGACAT CAGCCCTGGG 
GACCCATGTC CGGCCATTGA AGGCCCCACC TACACCTTTT ACACCCAGCT GCCTGAGACC 
ATAAACTCCT GCTGCAGAGG GGTGCGGTGT GCCTGCTTCT CCAGGGATGA CAAGCATGTA 
TTTGCAGGCA TGGAGGACCG CTCTGTCACT GCCTGGAGCA CGGTGGATGG CACTCTTTTG 
GCTGTCCAGT TTGTTCATGC TGTGATCAAC CGAATCATCC CTACCTCCAA TGGCTTCATG 
GCCCCCACAA GCCACGGCTA TCTCATCCGT GAGCGCTTCC AGTGCCCGTC AGTCAGAGCC 
TCCCAGCAAG ACCCTCTCAA GAACTTCAAG AAGGCAGTGT GGCTGGTAAA GACCAGGCAG 
AGGGAAGAGC TGGCTGCAGC AGAAGCCTCC CAGGATGCAG AACCAGTGGC CGTAGAGGGA 
AAGGAATCAA AGTCAAATAA ACGCTCACAG GTCTGCCTCA TACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Nwd1
Entrez Gene ID	319555
Full Name	NACHT and WD repeat domain containing 1
Synonyms	A230063L24Rik
General protein information	Preferred Names NACHT and WD repeat domain containing 1 Names NACHT domain- and WD repeat-containing protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 B3.3

	LOC719640	XP_001114186.1
Canis lupus familiaris (dog)	NWD1	XP_533883.4
Xenopus tropicalis (tropical clawed frog)	nwd1	XP_002934424.2
Homo sapiens (human)	NWD1	NP_001007526.3
Pan troglodytes (chimpanzee)	NWD1	XP_003316227.2
Mus musculus (house mouse)	Nwd1	NP_795914.3
Bos taurus (cattle)	NWD1	NP_001095680.1
Rattus norvegicus (Norway rat)	LOC100364350	XP_006252881.1
Danio rerio (zebrafish)	LOC100330908	XP_002663751.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Nwd1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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