AFF4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFF4
Entrez Gene ID	27125
Full Name	AF4/FMR2 family member 4
Synonyms	AF5Q31,CHOPS,MCEF
General protein information	Preferred Names AF4/FMR2 family member 4 Names AF4/FMR2 family member 4 ALL1-fused gene from chromosome 5q31 protein major CDK9 elongation factor-associated protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.1
Summary	The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). [provided by RefSeq, Oct 2011].
Disorder MIM:	604417
Disorder Html:	CHOPS syndrome, 616368 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006714587.3	XP_006714650.1	AF4/FMR2 family member 4 isoform X2
XM_005271963.4	XP_005272020.1	AF4/FMR2 family member 4 isoform X1
XM_006714587.4	XP_006714650.1	AF4/FMR2 family member 4 isoform X2
XM_005271964.4	XP_005272021.1	AF4/FMR2 family member 4 isoform X3
NM_014423.4	NP_055238.1	AF4/FMR2 family member 4
NM_014423.3	NP_055238.1	AF4/FMR2 family member 4
XM_005271963.5	XP_005272020.1	AF4/FMR2 family member 4 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AFF4	XP_517928.2
Canis lupus familiaris (dog)	AFF4	XP_850309.1
Mus musculus (house mouse)	Aff4	NP_291043.1
Gallus gallus (chicken)	AFF4	XP_004945088.1
Danio rerio (zebrafish)	aff4	XP_684327.6
Rattus norvegicus (Norway rat)	Aff4	NP_001100471.1
Xenopus tropicalis (tropical clawed frog)	aff4	XP_002935317.2
Homo sapiens (human)	AFF4	NP_055238.1
Bos taurus (cattle)	AFF4	NP_001178058.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Structural basis for ELL2 and AFF4 activation of HIV-1 proviral transcription.
Qi S, Li Z, Schulze-Gahmen U, Stjepanovic G, Zhou Q, Hurley JH
Nature communications814076(2017 Jan)

Insights into HIV-1 proviral transcription from integrative structure and dynamics of the Tat:AFF4:P-TEFb:TAR complex.
Schulze-Gahmen U, Echeverria I, Stjepanovic G, Bai Y, Lu H, Schneidman-Duhovny D, Doudna JA, Zhou Q, Sali A, Hurley JH
eLife5(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The TAR central loop contacts the CycT1 Tat-TAR recognition motif (TRM) and the second Tat Zn(2+)-binding loop. Hydrogen-deuterium exchange (HDX) shows that AFF4 helix 2 is stabilized in the TAR complex despite not touching the RNA, explaining how it enhances TAR binding to the SEC 50-fold.
Title: Insights into HIV-1 proviral transcription from integrative structure and dynamics of the Tat:AFF4:P-TEFb:TAR complex.

missense mutations in AFF4 in three unrelated probands with a new syndrome that phenotypically overlaps Cornelia de Lange syndrome (CdLS) that was named CHOPS syndrome (C for cognitive impairment and coarse facies, H for heart defects, O for obesity, P for pulmonary involvement and S for short stature and skeletal dysplasia)
Title: Germline gain-of-function mutations in AFF4 cause a developmental syndrome functionally linking the super elongation complex and cohesin.

AFF4 is positioned to make unexpected direct contacts with HIV Tat, and Tat enhances P-TEFb/CCNT1 affinity for AFF4.
Title: The AFF4 scaffold binds human P-TEFb adjacent to HIV Tat.

HIV-1 Tat recruits transcription elongation factors dispersed along a flexible AFF4 scaffold.
Title: HIV-1 Tat recruits transcription elongation factors dispersed along a flexible AFF4 scaffold.

overexpression of AFF2/3/4 interferes with the organization and/or biogenesis of nuclear speckles.
Title: Functional characterization of the AFF (AF4/FMR2) family of RNA-binding proteins: insights into the molecular pathology of FRAXE intellectual disability.

The following AFF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu39545	XM_005271964.4 Latest version!	Homo sapiens AF4/FMR2 family member 4 (AFF4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu18943	NM_014423.4 Latest version!	Homo sapiens AF4/FMR2 family member 4 (AFF4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu18943	XM_005271963.5 Latest version!	Homo sapiens AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu39544	XM_006714587.4 Latest version!	Homo sapiens AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu39544	XM_006714587.3	Homo sapiens AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu18943	NM_014423.3	Homo sapiens AF4/FMR2 family member 4 (AFF4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu18943	XM_005271963.4	Homo sapiens AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFF4 ( XM_005271964.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272021.1 Homo sapiens AF4/FMR2 family member 4 (AFF4), transcript variant X3, mRNA.

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CloneID	OHu39545
Clone ID Related Accession (Same CDS sequence)	XM_005271964.4
Accession Version	XM_005271964.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	AF4/FMR2 family member 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005271964.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAAAG ATGACTTAAA ACTAAGCAGC AGTGAAGACA GTGATGGGGA ACAGGATTGT 
GATAAGACAA TGCCGAGGAG TACACCAGGA AGTAACTCTG AACCTTCACA CCATAATAGT 
GAAGGAGCAG ATAACTCCAG GGATGATTCT AGTAGCCACA GTGGATCTGA AAGCAGCTCT 
GGATCTGACT CAGAGAGTGA AAGTAGTTCC AGTGACAGTG AGGCAAATGA GCCATCCCAG 
AGTGCATCTC CCGAGCCTGA ACCCCCGCCA ACAAACAAAT GGCAACTTGA TAATTGGCTG 
AATAAAGTGA ACCCACATAA AGTGTCACCC GCCTCTTCAG TGGACAGTAA CATCCCATCA 
TCTCAAGGCT ACAAAAAGGA AGGCCGAGAG CAGGGCACTG GGAATAGCTA CACTGATACA 
AGTGGACCTA AAGAAACGAG TTCCGCTACT CCGGGACGAG ACTCCAAAAC CATCCAAAAG 
GGATCAGAAA GTGGGCGTGG GAGGCAGAAA TCTCCTGCAC AGAGTGACAG CACAACACAG 
AGAAGAACTG TAGGCAAAAA ACAACCCAAA AAGGCTGAGA AGGCAGCTGC TGAAGAGCCT 
CGTGGAGGCC TGAAGATAGA AAGTGAAACC CCTGTAGACT TGGCTAGCAG CATGCCCTCC 
AGCAGACACA AAGCAGCCAC CAAAGGCTCA AGGAAACCCA ATATAAAGAA GGAGTCTAAG 
TCTTCCCCTC GACCTACAGC AGAGAAAAAG AAATATAAGT CAACAAGTAA ATCTTCCCAG 
AAATCAAGGG AAATCATAGA AACAGATACC TCATCCTCAG ATTCAGATGA AAGTGAGAGC 
CTTCCTCCTT CCTCACAAAC TCCTAAGTAC CCCGAGAGCA ATAGGACTCC TGTTAAACCC 
TCCTCAGTGG AGGAAGAAGA TAGCTTTTTT CGGCAACGAA TGTTCTCTCC TATGGAAGAG 
AAGGAACTTC TTTCACCCCT CAGTGAGCCT GATGACAGGT ACCCACTTAT TGTGAAGATT 
GACCTGAATC TTTTGACTAG AATACCAGGA AAGCCTTACA AAGAAACAGA GCCGCCCAAG 
GGGGAAAAGA AAAATGTGCC AGAAAAGCAC ACGAGAGAGG CTCAGAAACA AGCCTCAGAA 
AAAGTTTCCA ACAAAGGCAA GAGGAAGCAT AAGAATGAAG ATGATAACCG AGCCAGTGAG 
AGCAAGAAAC CCAAAACGGA GGACAAGAAT TCAGCAGGCC ATAAGCCATC CAGCAACAGA 
GAGTCATCTA AGCAGAGTGC TGCAAAAGAA AAGGATTTGT TGCCTTCTCC CGCTGGGCCT 
GTTCCTTCAA AAGATCCAAA AACAGAGCAT GGCTCTCGGA AGAGGACTAT TAGTCAGTCT 
TCTTCCTTAA AGTCAAGCAG TAACAGCAAC AAGGAGACGA GTGGCAGCAG CAAAAACAGT 
TCCTCCACAT CAAAGCAGAA GAAGACCGAA GGGAAGACTT CCAGTAGCTC CAAGGAGGTT 
AAGGAAAAGG CTCCAAGTAG CTCCTCTAAC TGTCCTCCAT CTGCACCAAC TCTTGATTCT 
TCTAAGCCTC GGAGAACAAA GCTTGTCTTT GATGACAGAA ATTATTCAGC AGACCATTAT 
TTACAAGAAG CAAAAAAGCT AAAGCACAAT GCAGATGCAT TGTCTGATAG GTTTGAGAAA 
GCTGTATACT ATCTTGATGC TGTGGTATCT TTCATTGAAT GTGGGAATGC ATTAGAGAAG 
AATGCTCAGG AATCCAAATC CCCATTCCCT ATGTATTCAG AGACGGTGGA TCTCATCAAA 
TACACTATGA AGCTAAAGAA TTACTTGGCA CCAGATGCTA CAGCTGCAGA TAAACGACTC 
ACAGTACTTT GCCTGCGATG CGAGTCTTTG CTGTACCTGA GGCTGTTCAA ACTGAAGAAG 
GAAAATGCTC TGAAGTACTC AAAGACACTG ACAGAGCACC TGAAGAATTC TTATAATAAT 
TCTCAAGCAC CATCGCCTGG CTTGGGAAGC AAAGCTGTGG GGATGCCTTC CCCTGTTTCT 
CCAAAGCTGT CACCAGGCAA TTCAGGAAAT TATTCATCTG GGGCCAGTAG TGCTTCTGCA 
AGTGGTTCTT CAGTGACCAT TCCACAGAAG ATCCACCAGA TGGCAGCCAG CTATGTTCAG 
GTCACATCCA ACTTCCTCTA TGCCACCGAA ATTTGGGACC AAGCTGAACA GCTTTCCAAA 
GAGCAAAAAG AATTCTTTGC TGAACTGGAT AAAGTAATGG GCCCTCTCAT CTTTAATGCA 
AGCATCATGA CAGATCTAGT TCGTTATACC CGGCAGGGAC TGCACTGGCT TCGCCAGGAT 
GCCAAGTTGA TATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272021.1
CDS	255..2612
Translation	MLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDTSGPKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKAEKAAAEEPRGGLKIESETPVDLASSMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRASESKKPKTEDKNSAGHKPSSNRESSKQSAAKEKDLLPSPAGPVPSKDPKTEHGSRKRTISQSSSLKSSSNSNKETSGSSKNSSSTSKQKKTEGKTSSSSKEVKEKAPSSSSNCPPSAPTLDSSKPRRTKLVFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSETVDLIKYTMKLKNYLAPDATAADKRLTVLCLRCESLLYLRLFKLKKENALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	XM_005271964.4
Organism	Homo sapiens(human)
Definition	Homo sapiens AF4/FMR2 family member 4 (AFF4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271964.4

ORF Insert Sequence:

ATGTTAAAAG ATGACTTAAA ACTAAGCAGC AGTGAAGACA GTGATGGGGA ACAGGATTGT 
GATAAGACAA TGCCGAGGAG TACACCAGGA AGTAACTCTG AACCTTCACA CCATAATAGT 
GAAGGAGCAG ATAACTCCAG GGATGATTCT AGTAGCCACA GTGGATCTGA AAGCAGCTCT 
GGATCTGACT CAGAGAGTGA AAGTAGTTCC AGTGACAGTG AGGCAAATGA GCCATCCCAG 
AGTGCATCTC CCGAGCCTGA ACCCCCGCCA ACAAACAAAT GGCAACTTGA TAATTGGCTG 
AATAAAGTGA ACCCACATAA AGTGTCACCC GCCTCTTCAG TGGACAGTAA CATCCCATCA 
TCTCAAGGCT ACAAAAAGGA AGGCCGAGAG CAGGGCACTG GGAATAGCTA CACTGATACA 
AGTGGACCTA AAGAAACGAG TTCCGCTACT CCGGGACGAG ACTCCAAAAC CATCCAAAAG 
GGATCAGAAA GTGGGCGTGG GAGGCAGAAA TCTCCTGCAC AGAGTGACAG CACAACACAG 
AGAAGAACTG TAGGCAAAAA ACAACCCAAA AAGGCTGAGA AGGCAGCTGC TGAAGAGCCT 
CGTGGAGGCC TGAAGATAGA AAGTGAAACC CCTGTAGACT TGGCTAGCAG CATGCCCTCC 
AGCAGACACA AAGCAGCCAC CAAAGGCTCA AGGAAACCCA ATATAAAGAA GGAGTCTAAG 
TCTTCCCCTC GACCTACAGC AGAGAAAAAG AAATATAAGT CAACAAGTAA ATCTTCCCAG 
AAATCAAGGG AAATCATAGA AACAGATACC TCATCCTCAG ATTCAGATGA AAGTGAGAGC 
CTTCCTCCTT CCTCACAAAC TCCTAAGTAC CCCGAGAGCA ATAGGACTCC TGTTAAACCC 
TCCTCAGTGG AGGAAGAAGA TAGCTTTTTT CGGCAACGAA TGTTCTCTCC TATGGAAGAG 
AAGGAACTTC TTTCACCCCT CAGTGAGCCT GATGACAGGT ACCCACTTAT TGTGAAGATT 
GACCTGAATC TTTTGACTAG AATACCAGGA AAGCCTTACA AAGAAACAGA GCCGCCCAAG 
GGGGAAAAGA AAAATGTGCC AGAAAAGCAC ACGAGAGAGG CTCAGAAACA AGCCTCAGAA 
AAAGTTTCCA ACAAAGGCAA GAGGAAGCAT AAGAATGAAG ATGATAACCG AGCCAGTGAG 
AGCAAGAAAC CCAAAACGGA GGACAAGAAT TCAGCAGGCC ATAAGCCATC CAGCAACAGA 
GAGTCATCTA AGCAGAGTGC TGCAAAAGAA AAGGATTTGT TGCCTTCTCC CGCTGGGCCT 
GTTCCTTCAA AAGATCCAAA AACAGAGCAT GGCTCTCGGA AGAGGACTAT TAGTCAGTCT 
TCTTCCTTAA AGTCAAGCAG TAACAGCAAC AAGGAGACGA GTGGCAGCAG CAAAAACAGT 
TCCTCCACAT CAAAGCAGAA GAAGACCGAA GGGAAGACTT CCAGTAGCTC CAAGGAGGTT 
AAGGAAAAGG CTCCAAGTAG CTCCTCTAAC TGTCCTCCAT CTGCACCAAC TCTTGATTCT 
TCTAAGCCTC GGAGAACAAA GCTTGTCTTT GATGACAGAA ATTATTCAGC AGACCATTAT 
TTACAAGAAG CAAAAAAGCT AAAGCACAAT GCAGATGCAT TGTCTGATAG GTTTGAGAAA 
GCTGTATACT ATCTTGATGC TGTGGTATCT TTCATTGAAT GTGGGAATGC ATTAGAGAAG 
AATGCTCAGG AATCCAAATC CCCATTCCCT ATGTATTCAG AGACGGTGGA TCTCATCAAA 
TACACTATGA AGCTAAAGAA TTACTTGGCA CCAGATGCTA CAGCTGCAGA TAAACGACTC 
ACAGTACTTT GCCTGCGATG CGAGTCTTTG CTGTACCTGA GGCTGTTCAA ACTGAAGAAG 
GAAAATGCTC TGAAGTACTC AAAGACACTG ACAGAGCACC TGAAGAATTC TTATAATAAT 
TCTCAAGCAC CATCGCCTGG CTTGGGAAGC AAAGCTGTGG GGATGCCTTC CCCTGTTTCT 
CCAAAGCTGT CACCAGGCAA TTCAGGAAAT TATTCATCTG GGGCCAGTAG TGCTTCTGCA 
AGTGGTTCTT CAGTGACCAT TCCACAGAAG ATCCACCAGA TGGCAGCCAG CTATGTTCAG 
GTCACATCCA ACTTCCTCTA TGCCACCGAA ATTTGGGACC AAGCTGAACA GCTTTCCAAA 
GAGCAAAAAG AATTCTTTGC TGAACTGGAT AAAGTAATGG GCCCTCTCAT CTTTAATGCA 
AGCATCATGA CAGATCTAGT TCGTTATACC CGGCAGGGAC TGCACTGGCT TCGCCAGGAT 
GCCAAGTTGA TATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( NM_014423.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055238.1 Homo sapiens AF4/FMR2 family member 4 (AFF4), mRNA.

Price & Availability↑

CloneID	OHu18943
Clone ID Related Accession (Same CDS sequence)	XM_005271963.4 , NM_014423.4 , NM_014423.3 , XM_005271963.5
Accession Version	NM_014423.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	AF4/FMR2 family member 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA934874.1, BC100287.1, AF197927.1 and AC004500.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_014423.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC137226.1, AF213987.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265343.10/ ENSP00000265343.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGAAGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCAGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GTTATCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCAC CATCAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGA 
GGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCCG CACCCAGCAC TTCTCAGTCT 
CAGAAACGGT CCTCAGGCTT ACAGAGTGGA CATAGTAGCC AGCGGACCAG CGCAGGTAGC 
AGTAGTGGCA CTAACAGTAG TGGTCAGAGG CACGACCGTG AGTCATATAA CAATAGTGGG 
AGCAGTAGCC GGAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG TTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AACTCTAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAGGAACA TCAACGCTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAACTCAATC TTTCCCACCC 
TCATTGATGT CAAAGTCCAA TTCAATGTTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GACGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAGC ACTACAGCAG CCAATCCCAT 
GGCAACAGCA TGACTGAGCT GAAGCCCAGC AGCAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CACTGGATGC ATCAGCTTCT GGTGATGTGA GCTGTGTGGA TGAAATCCTA 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAATTT TGGCACTGGA 
GAACAAAAAA GATATAATCC TTCTAAAACT TCAAATGGGC ACCAGTCTAA ATCTATGTTA 
AAAGATGACT TAAAACTAAG CAGCAGTGAA GACAGTGATG GGGAACAGGA TTGTGATAAG 
ACAATGCCGA GGAGTACACC AGGAAGTAAC TCTGAACCTT CACACCATAA TAGTGAAGGA 
GCAGATAACT CCAGGGATGA TTCTAGTAGC CACAGTGGAT CTGAAAGCAG CTCTGGATCT 
GACTCAGAGA GTGAAAGTAG TTCCAGTGAC AGTGAGGCAA ATGAGCCATC CCAGAGTGCA 
TCTCCCGAGC CTGAACCCCC GCCAACAAAC AAATGGCAAC TTGATAATTG GCTGAATAAA 
GTGAACCCAC ATAAAGTGTC ACCCGCCTCT TCAGTGGACA GTAACATCCC ATCATCTCAA 
GGCTACAAAA AGGAAGGCCG AGAGCAGGGC ACTGGGAATA GCTACACTGA TACAAGTGGA 
CCTAAAGAAA CGAGTTCCGC TACTCCGGGA CGAGACTCCA AAACCATCCA AAAGGGATCA 
GAAAGTGGGC GTGGGAGGCA GAAATCTCCT GCACAGAGTG ACAGCACAAC ACAGAGAAGA 
ACTGTAGGCA AAAAACAACC CAAAAAGGCT GAGAAGGCAG CTGCTGAAGA GCCTCGTGGA 
GGCCTGAAGA TAGAAAGTGA AACCCCTGTA GACTTGGCTA GCAGCATGCC CTCCAGCAGA 
CACAAAGCAG CCACCAAAGG CTCAAGGAAA CCCAATATAA AGAAGGAGTC TAAGTCTTCC 
CCTCGACCTA CAGCAGAGAA AAAGAAATAT AAGTCAACAA GTAAATCTTC CCAGAAATCA 
AGGGAAATCA TAGAAACAGA TACCTCATCC TCAGATTCAG ATGAAAGTGA GAGCCTTCCT 
CCTTCCTCAC AAACTCCTAA GTACCCCGAG AGCAATAGGA CTCCTGTTAA ACCCTCCTCA 
GTGGAGGAAG AAGATAGCTT TTTTCGGCAA CGAATGTTCT CTCCTATGGA AGAGAAGGAA 
CTTCTTTCAC CCCTCAGTGA GCCTGATGAC AGGTACCCAC TTATTGTGAA GATTGACCTG 
AATCTTTTGA CTAGAATACC AGGAAAGCCT TACAAAGAAA CAGAGCCGCC CAAGGGGGAA 
AAGAAAAATG TGCCAGAAAA GCACACGAGA GAGGCTCAGA AACAAGCCTC AGAAAAAGTT 
TCCAACAAAG GCAAGAGGAA GCATAAGAAT GAAGATGATA ACCGAGCCAG TGAGAGCAAG 
AAACCCAAAA CGGAGGACAA GAATTCAGCA GGCCATAAGC CATCCAGCAA CAGAGAGTCA 
TCTAAGCAGA GTGCTGCAAA AGAAAAGGAT TTGTTGCCTT CTCCCGCTGG GCCTGTTCCT 
TCAAAAGATC CAAAAACAGA GCATGGCTCT CGGAAGAGGA CTATTAGTCA GTCTTCTTCC 
TTAAAGTCAA GCAGTAACAG CAACAAGGAG ACGAGTGGCA GCAGCAAAAA CAGTTCCTCC 
ACATCAAAGC AGAAGAAGAC CGAAGGGAAG ACTTCCAGTA GCTCCAAGGA GGTTAAGGAA 
AAGGCTCCAA GTAGCTCCTC TAACTGTCCT CCATCTGCAC CAACTCTTGA TTCTTCTAAG 
CCTCGGAGAA CAAAGCTTGT CTTTGATGAC AGAAATTATT CAGCAGACCA TTATTTACAA 
GAAGCAAAAA AGCTAAAGCA CAATGCAGAT GCATTGTCTG ATAGGTTTGA GAAAGCTGTA 
TACTATCTTG ATGCTGTGGT ATCTTTCATT GAATGTGGGA ATGCATTAGA GAAGAATGCT 
CAGGAATCCA AATCCCCATT CCCTATGTAT TCAGAGACGG TGGATCTCAT CAAATACACT 
ATGAAGCTAA AGAATTACTT GGCACCAGAT GCTACAGCTG CAGATAAACG ACTCACAGTA 
CTTTGCCTGC GATGCGAGTC TTTGCTGTAC CTGAGGCTGT TCAAACTGAA GAAGGAAAAT 
GCTCTGAAGT ACTCAAAGAC ACTGACAGAG CACCTGAAGA ATTCTTATAA TAATTCTCAA 
GCACCATCGC CTGGCTTGGG AAGCAAAGCT GTGGGGATGC CTTCCCCTGT TTCTCCAAAG 
CTGTCACCAG GCAATTCAGG AAATTATTCA TCTGGGGCCA GTAGTGCTTC TGCAAGTGGT 
TCTTCAGTGA CCATTCCACA GAAGATCCAC CAGATGGCAG CCAGCTATGT TCAGGTCACA 
TCCAACTTCC TCTATGCCAC CGAAATTTGG GACCAAGCTG AACAGCTTTC CAAAGAGCAA 
AAAGAATTCT TTGCTGAACT GGATAAAGTA ATGGGCCCTC TCATCTTTAA TGCAAGCATC 
ATGACAGATC TAGTTCGTTA TACCCGGCAG GGACTGCACT GGCTTCGCCA GGATGCCAAG 
TTGATATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055238.1
CDS	381..3872
Translation	MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQKRYNPSKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDTSGPKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKAEKAAAEEPRGGLKIESETPVDLASSMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRASESKKPKTEDKNSAGHKPSSNRESSKQSAAKEKDLLPSPAGPVPSKDPKTEHGSRKRTISQSSSLKSSSNSNKETSGSSKNSSSTSKQKKTEGKTSSSSKEVKEKAPSSSSNCPPSAPTLDSSKPRRTKLVFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSETVDLIKYTMKLKNYLAPDATAADKRLTVLCLRCESLLYLRLFKLKKENALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	NM_014423.4
Organism	Homo sapiens(human)
Definition	Homo sapiens AF4/FMR2 family member 4 (AFF4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014423.4

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGAAGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCAGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GTTATCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCAC CATCAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGA 
GGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCCG CACCCAGCAC TTCTCAGTCT 
CAGAAACGGT CCTCAGGCTT ACAGAGTGGA CATAGTAGCC AGCGGACCAG CGCAGGTAGC 
AGTAGTGGCA CTAACAGTAG TGGTCAGAGG CACGACCGTG AGTCATATAA CAATAGTGGG 
AGCAGTAGCC GGAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG TTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AACTCTAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAGGAACA TCAACGCTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAACTCAATC TTTCCCACCC 
TCATTGATGT CAAAGTCCAA TTCAATGTTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GACGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAGC ACTACAGCAG CCAATCCCAT 
GGCAACAGCA TGACTGAGCT GAAGCCCAGC AGCAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CACTGGATGC ATCAGCTTCT GGTGATGTGA GCTGTGTGGA TGAAATCCTA 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAATTT TGGCACTGGA 
GAACAAAAAA GATATAATCC TTCTAAAACT TCAAATGGGC ACCAGTCTAA ATCTATGTTA 
AAAGATGACT TAAAACTAAG CAGCAGTGAA GACAGTGATG GGGAACAGGA TTGTGATAAG 
ACAATGCCGA GGAGTACACC AGGAAGTAAC TCTGAACCTT CACACCATAA TAGTGAAGGA 
GCAGATAACT CCAGGGATGA TTCTAGTAGC CACAGTGGAT CTGAAAGCAG CTCTGGATCT 
GACTCAGAGA GTGAAAGTAG TTCCAGTGAC AGTGAGGCAA ATGAGCCATC CCAGAGTGCA 
TCTCCCGAGC CTGAACCCCC GCCAACAAAC AAATGGCAAC TTGATAATTG GCTGAATAAA 
GTGAACCCAC ATAAAGTGTC ACCCGCCTCT TCAGTGGACA GTAACATCCC ATCATCTCAA 
GGCTACAAAA AGGAAGGCCG AGAGCAGGGC ACTGGGAATA GCTACACTGA TACAAGTGGA 
CCTAAAGAAA CGAGTTCCGC TACTCCGGGA CGAGACTCCA AAACCATCCA AAAGGGATCA 
GAAAGTGGGC GTGGGAGGCA GAAATCTCCT GCACAGAGTG ACAGCACAAC ACAGAGAAGA 
ACTGTAGGCA AAAAACAACC CAAAAAGGCT GAGAAGGCAG CTGCTGAAGA GCCTCGTGGA 
GGCCTGAAGA TAGAAAGTGA AACCCCTGTA GACTTGGCTA GCAGCATGCC CTCCAGCAGA 
CACAAAGCAG CCACCAAAGG CTCAAGGAAA CCCAATATAA AGAAGGAGTC TAAGTCTTCC 
CCTCGACCTA CAGCAGAGAA AAAGAAATAT AAGTCAACAA GTAAATCTTC CCAGAAATCA 
AGGGAAATCA TAGAAACAGA TACCTCATCC TCAGATTCAG ATGAAAGTGA GAGCCTTCCT 
CCTTCCTCAC AAACTCCTAA GTACCCCGAG AGCAATAGGA CTCCTGTTAA ACCCTCCTCA 
GTGGAGGAAG AAGATAGCTT TTTTCGGCAA CGAATGTTCT CTCCTATGGA AGAGAAGGAA 
CTTCTTTCAC CCCTCAGTGA GCCTGATGAC AGGTACCCAC TTATTGTGAA GATTGACCTG 
AATCTTTTGA CTAGAATACC AGGAAAGCCT TACAAAGAAA CAGAGCCGCC CAAGGGGGAA 
AAGAAAAATG TGCCAGAAAA GCACACGAGA GAGGCTCAGA AACAAGCCTC AGAAAAAGTT 
TCCAACAAAG GCAAGAGGAA GCATAAGAAT GAAGATGATA ACCGAGCCAG TGAGAGCAAG 
AAACCCAAAA CGGAGGACAA GAATTCAGCA GGCCATAAGC CATCCAGCAA CAGAGAGTCA 
TCTAAGCAGA GTGCTGCAAA AGAAAAGGAT TTGTTGCCTT CTCCCGCTGG GCCTGTTCCT 
TCAAAAGATC CAAAAACAGA GCATGGCTCT CGGAAGAGGA CTATTAGTCA GTCTTCTTCC 
TTAAAGTCAA GCAGTAACAG CAACAAGGAG ACGAGTGGCA GCAGCAAAAA CAGTTCCTCC 
ACATCAAAGC AGAAGAAGAC CGAAGGGAAG ACTTCCAGTA GCTCCAAGGA GGTTAAGGAA 
AAGGCTCCAA GTAGCTCCTC TAACTGTCCT CCATCTGCAC CAACTCTTGA TTCTTCTAAG 
CCTCGGAGAA CAAAGCTTGT CTTTGATGAC AGAAATTATT CAGCAGACCA TTATTTACAA 
GAAGCAAAAA AGCTAAAGCA CAATGCAGAT GCATTGTCTG ATAGGTTTGA GAAAGCTGTA 
TACTATCTTG ATGCTGTGGT ATCTTTCATT GAATGTGGGA ATGCATTAGA GAAGAATGCT 
CAGGAATCCA AATCCCCATT CCCTATGTAT TCAGAGACGG TGGATCTCAT CAAATACACT 
ATGAAGCTAA AGAATTACTT GGCACCAGAT GCTACAGCTG CAGATAAACG ACTCACAGTA 
CTTTGCCTGC GATGCGAGTC TTTGCTGTAC CTGAGGCTGT TCAAACTGAA GAAGGAAAAT 
GCTCTGAAGT ACTCAAAGAC ACTGACAGAG CACCTGAAGA ATTCTTATAA TAATTCTCAA 
GCACCATCGC CTGGCTTGGG AAGCAAAGCT GTGGGGATGC CTTCCCCTGT TTCTCCAAAG 
CTGTCACCAG GCAATTCAGG AAATTATTCA TCTGGGGCCA GTAGTGCTTC TGCAAGTGGT 
TCTTCAGTGA CCATTCCACA GAAGATCCAC CAGATGGCAG CCAGCTATGT TCAGGTCACA 
TCCAACTTCC TCTATGCCAC CGAAATTTGG GACCAAGCTG AACAGCTTTC CAAAGAGCAA 
AAAGAATTCT TTGCTGAACT GGATAAAGTA ATGGGCCCTC TCATCTTTAA TGCAAGCATC 
ATGACAGATC TAGTTCGTTA TACCCGGCAG GGACTGCACT GGCTTCGCCA GGATGCCAAG 
TTGATATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( NM_014423.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055238.1 Homo sapiens AF4/FMR2 family member 4 (AFF4), mRNA.

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CloneID	OHu18943
Clone ID Related Accession (Same CDS sequence)	XM_005271963.4 , NM_014423.4 , NM_014423.3 , XM_005271963.5
Accession Version	NM_014423.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	AF4/FMR2 family member 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA934874.1, BC100287.1, AF197927.1 and AC004500.1. This sequence is a reference standard in the RefSeqGene project. On May 18, 2006 this sequence version replaced NM_014423.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC137226.1, AF213987.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGAAGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCAGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GTTATCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCAC CATCAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGA 
GGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCCG CACCCAGCAC TTCTCAGTCT 
CAGAAACGGT CCTCAGGCTT ACAGAGTGGA CATAGTAGCC AGCGGACCAG CGCAGGTAGC 
AGTAGTGGCA CTAACAGTAG TGGTCAGAGG CACGACCGTG AGTCATATAA CAATAGTGGG 
AGCAGTAGCC GGAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG TTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AACTCTAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAGGAACA TCAACGCTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAACTCAATC TTTCCCACCC 
TCATTGATGT CAAAGTCCAA TTCAATGTTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GACGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAGC ACTACAGCAG CCAATCCCAT 
GGCAACAGCA TGACTGAGCT GAAGCCCAGC AGCAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CACTGGATGC ATCAGCTTCT GGTGATGTGA GCTGTGTGGA TGAAATCCTA 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAATTT TGGCACTGGA 
GAACAAAAAA GATATAATCC TTCTAAAACT TCAAATGGGC ACCAGTCTAA ATCTATGTTA 
AAAGATGACT TAAAACTAAG CAGCAGTGAA GACAGTGATG GGGAACAGGA TTGTGATAAG 
ACAATGCCGA GGAGTACACC AGGAAGTAAC TCTGAACCTT CACACCATAA TAGTGAAGGA 
GCAGATAACT CCAGGGATGA TTCTAGTAGC CACAGTGGAT CTGAAAGCAG CTCTGGATCT 
GACTCAGAGA GTGAAAGTAG TTCCAGTGAC AGTGAGGCAA ATGAGCCATC CCAGAGTGCA 
TCTCCCGAGC CTGAACCCCC GCCAACAAAC AAATGGCAAC TTGATAATTG GCTGAATAAA 
GTGAACCCAC ATAAAGTGTC ACCCGCCTCT TCAGTGGACA GTAACATCCC ATCATCTCAA 
GGCTACAAAA AGGAAGGCCG AGAGCAGGGC ACTGGGAATA GCTACACTGA TACAAGTGGA 
CCTAAAGAAA CGAGTTCCGC TACTCCGGGA CGAGACTCCA AAACCATCCA AAAGGGATCA 
GAAAGTGGGC GTGGGAGGCA GAAATCTCCT GCACAGAGTG ACAGCACAAC ACAGAGAAGA 
ACTGTAGGCA AAAAACAACC CAAAAAGGCT GAGAAGGCAG CTGCTGAAGA GCCTCGTGGA 
GGCCTGAAGA TAGAAAGTGA AACCCCTGTA GACTTGGCTA GCAGCATGCC CTCCAGCAGA 
CACAAAGCAG CCACCAAAGG CTCAAGGAAA CCCAATATAA AGAAGGAGTC TAAGTCTTCC 
CCTCGACCTA CAGCAGAGAA AAAGAAATAT AAGTCAACAA GTAAATCTTC CCAGAAATCA 
AGGGAAATCA TAGAAACAGA TACCTCATCC TCAGATTCAG ATGAAAGTGA GAGCCTTCCT 
CCTTCCTCAC AAACTCCTAA GTACCCCGAG AGCAATAGGA CTCCTGTTAA ACCCTCCTCA 
GTGGAGGAAG AAGATAGCTT TTTTCGGCAA CGAATGTTCT CTCCTATGGA AGAGAAGGAA 
CTTCTTTCAC CCCTCAGTGA GCCTGATGAC AGGTACCCAC TTATTGTGAA GATTGACCTG 
AATCTTTTGA CTAGAATACC AGGAAAGCCT TACAAAGAAA CAGAGCCGCC CAAGGGGGAA 
AAGAAAAATG TGCCAGAAAA GCACACGAGA GAGGCTCAGA AACAAGCCTC AGAAAAAGTT 
TCCAACAAAG GCAAGAGGAA GCATAAGAAT GAAGATGATA ACCGAGCCAG TGAGAGCAAG 
AAACCCAAAA CGGAGGACAA GAATTCAGCA GGCCATAAGC CATCCAGCAA CAGAGAGTCA 
TCTAAGCAGA GTGCTGCAAA AGAAAAGGAT TTGTTGCCTT CTCCCGCTGG GCCTGTTCCT 
TCAAAAGATC CAAAAACAGA GCATGGCTCT CGGAAGAGGA CTATTAGTCA GTCTTCTTCC 
TTAAAGTCAA GCAGTAACAG CAACAAGGAG ACGAGTGGCA GCAGCAAAAA CAGTTCCTCC 
ACATCAAAGC AGAAGAAGAC CGAAGGGAAG ACTTCCAGTA GCTCCAAGGA GGTTAAGGAA 
AAGGCTCCAA GTAGCTCCTC TAACTGTCCT CCATCTGCAC CAACTCTTGA TTCTTCTAAG 
CCTCGGAGAA CAAAGCTTGT CTTTGATGAC AGAAATTATT CAGCAGACCA TTATTTACAA 
GAAGCAAAAA AGCTAAAGCA CAATGCAGAT GCATTGTCTG ATAGGTTTGA GAAAGCTGTA 
TACTATCTTG ATGCTGTGGT ATCTTTCATT GAATGTGGGA ATGCATTAGA GAAGAATGCT 
CAGGAATCCA AATCCCCATT CCCTATGTAT TCAGAGACGG TGGATCTCAT CAAATACACT 
ATGAAGCTAA AGAATTACTT GGCACCAGAT GCTACAGCTG CAGATAAACG ACTCACAGTA 
CTTTGCCTGC GATGCGAGTC TTTGCTGTAC CTGAGGCTGT TCAAACTGAA GAAGGAAAAT 
GCTCTGAAGT ACTCAAAGAC ACTGACAGAG CACCTGAAGA ATTCTTATAA TAATTCTCAA 
GCACCATCGC CTGGCTTGGG AAGCAAAGCT GTGGGGATGC CTTCCCCTGT TTCTCCAAAG 
CTGTCACCAG GCAATTCAGG AAATTATTCA TCTGGGGCCA GTAGTGCTTC TGCAAGTGGT 
TCTTCAGTGA CCATTCCACA GAAGATCCAC CAGATGGCAG CCAGCTATGT TCAGGTCACA 
TCCAACTTCC TCTATGCCAC CGAAATTTGG GACCAAGCTG AACAGCTTTC CAAAGAGCAA 
AAAGAATTCT TTGCTGAACT GGATAAAGTA ATGGGCCCTC TCATCTTTAA TGCAAGCATC 
ATGACAGATC TAGTTCGTTA TACCCGGCAG GGACTGCACT GGCTTCGCCA GGATGCCAAG 
TTGATATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055238.1
CDS	409..3900
Translation	MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQKRYNPSKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDTSGPKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKAEKAAAEEPRGGLKIESETPVDLASSMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRASESKKPKTEDKNSAGHKPSSNRESSKQSAAKEKDLLPSPAGPVPSKDPKTEHGSRKRTISQSSSLKSSSNSNKETSGSSKNSSSTSKQKKTEGKTSSSSKEVKEKAPSSSSNCPPSAPTLDSSKPRRTKLVFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSETVDLIKYTMKLKNYLAPDATAADKRLTVLCLRCESLLYLRLFKLKKENALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	NM_014423.3
Organism	Homo sapiens(human)
Definition	Homo sapiens AF4/FMR2 family member 4 (AFF4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014423.3

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGAAGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCAGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GTTATCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCAC CATCAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGA 
GGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCCG CACCCAGCAC TTCTCAGTCT 
CAGAAACGGT CCTCAGGCTT ACAGAGTGGA CATAGTAGCC AGCGGACCAG CGCAGGTAGC 
AGTAGTGGCA CTAACAGTAG TGGTCAGAGG CACGACCGTG AGTCATATAA CAATAGTGGG 
AGCAGTAGCC GGAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG TTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AACTCTAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAGGAACA TCAACGCTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAACTCAATC TTTCCCACCC 
TCATTGATGT CAAAGTCCAA TTCAATGTTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GACGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAGC ACTACAGCAG CCAATCCCAT 
GGCAACAGCA TGACTGAGCT GAAGCCCAGC AGCAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CACTGGATGC ATCAGCTTCT GGTGATGTGA GCTGTGTGGA TGAAATCCTA 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAATTT TGGCACTGGA 
GAACAAAAAA GATATAATCC TTCTAAAACT TCAAATGGGC ACCAGTCTAA ATCTATGTTA 
AAAGATGACT TAAAACTAAG CAGCAGTGAA GACAGTGATG GGGAACAGGA TTGTGATAAG 
ACAATGCCGA GGAGTACACC AGGAAGTAAC TCTGAACCTT CACACCATAA TAGTGAAGGA 
GCAGATAACT CCAGGGATGA TTCTAGTAGC CACAGTGGAT CTGAAAGCAG CTCTGGATCT 
GACTCAGAGA GTGAAAGTAG TTCCAGTGAC AGTGAGGCAA ATGAGCCATC CCAGAGTGCA 
TCTCCCGAGC CTGAACCCCC GCCAACAAAC AAATGGCAAC TTGATAATTG GCTGAATAAA 
GTGAACCCAC ATAAAGTGTC ACCCGCCTCT TCAGTGGACA GTAACATCCC ATCATCTCAA 
GGCTACAAAA AGGAAGGCCG AGAGCAGGGC ACTGGGAATA GCTACACTGA TACAAGTGGA 
CCTAAAGAAA CGAGTTCCGC TACTCCGGGA CGAGACTCCA AAACCATCCA AAAGGGATCA 
GAAAGTGGGC GTGGGAGGCA GAAATCTCCT GCACAGAGTG ACAGCACAAC ACAGAGAAGA 
ACTGTAGGCA AAAAACAACC CAAAAAGGCT GAGAAGGCAG CTGCTGAAGA GCCTCGTGGA 
GGCCTGAAGA TAGAAAGTGA AACCCCTGTA GACTTGGCTA GCAGCATGCC CTCCAGCAGA 
CACAAAGCAG CCACCAAAGG CTCAAGGAAA CCCAATATAA AGAAGGAGTC TAAGTCTTCC 
CCTCGACCTA CAGCAGAGAA AAAGAAATAT AAGTCAACAA GTAAATCTTC CCAGAAATCA 
AGGGAAATCA TAGAAACAGA TACCTCATCC TCAGATTCAG ATGAAAGTGA GAGCCTTCCT 
CCTTCCTCAC AAACTCCTAA GTACCCCGAG AGCAATAGGA CTCCTGTTAA ACCCTCCTCA 
GTGGAGGAAG AAGATAGCTT TTTTCGGCAA CGAATGTTCT CTCCTATGGA AGAGAAGGAA 
CTTCTTTCAC CCCTCAGTGA GCCTGATGAC AGGTACCCAC TTATTGTGAA GATTGACCTG 
AATCTTTTGA CTAGAATACC AGGAAAGCCT TACAAAGAAA CAGAGCCGCC CAAGGGGGAA 
AAGAAAAATG TGCCAGAAAA GCACACGAGA GAGGCTCAGA AACAAGCCTC AGAAAAAGTT 
TCCAACAAAG GCAAGAGGAA GCATAAGAAT GAAGATGATA ACCGAGCCAG TGAGAGCAAG 
AAACCCAAAA CGGAGGACAA GAATTCAGCA GGCCATAAGC CATCCAGCAA CAGAGAGTCA 
TCTAAGCAGA GTGCTGCAAA AGAAAAGGAT TTGTTGCCTT CTCCCGCTGG GCCTGTTCCT 
TCAAAAGATC CAAAAACAGA GCATGGCTCT CGGAAGAGGA CTATTAGTCA GTCTTCTTCC 
TTAAAGTCAA GCAGTAACAG CAACAAGGAG ACGAGTGGCA GCAGCAAAAA CAGTTCCTCC 
ACATCAAAGC AGAAGAAGAC CGAAGGGAAG ACTTCCAGTA GCTCCAAGGA GGTTAAGGAA 
AAGGCTCCAA GTAGCTCCTC TAACTGTCCT CCATCTGCAC CAACTCTTGA TTCTTCTAAG 
CCTCGGAGAA CAAAGCTTGT CTTTGATGAC AGAAATTATT CAGCAGACCA TTATTTACAA 
GAAGCAAAAA AGCTAAAGCA CAATGCAGAT GCATTGTCTG ATAGGTTTGA GAAAGCTGTA 
TACTATCTTG ATGCTGTGGT ATCTTTCATT GAATGTGGGA ATGCATTAGA GAAGAATGCT 
CAGGAATCCA AATCCCCATT CCCTATGTAT TCAGAGACGG TGGATCTCAT CAAATACACT 
ATGAAGCTAA AGAATTACTT GGCACCAGAT GCTACAGCTG CAGATAAACG ACTCACAGTA 
CTTTGCCTGC GATGCGAGTC TTTGCTGTAC CTGAGGCTGT TCAAACTGAA GAAGGAAAAT 
GCTCTGAAGT ACTCAAAGAC ACTGACAGAG CACCTGAAGA ATTCTTATAA TAATTCTCAA 
GCACCATCGC CTGGCTTGGG AAGCAAAGCT GTGGGGATGC CTTCCCCTGT TTCTCCAAAG 
CTGTCACCAG GCAATTCAGG AAATTATTCA TCTGGGGCCA GTAGTGCTTC TGCAAGTGGT 
TCTTCAGTGA CCATTCCACA GAAGATCCAC CAGATGGCAG CCAGCTATGT TCAGGTCACA 
TCCAACTTCC TCTATGCCAC CGAAATTTGG GACCAAGCTG AACAGCTTTC CAAAGAGCAA 
AAAGAATTCT TTGCTGAACT GGATAAAGTA ATGGGCCCTC TCATCTTTAA TGCAAGCATC 
ATGACAGATC TAGTTCGTTA TACCCGGCAG GGACTGCACT GGCTTCGCCA GGATGCCAAG 
TTGATATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_005271963.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272020.1 Homo sapiens AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.

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CloneID	OHu18943
Clone ID Related Accession (Same CDS sequence)	XM_005271963.4 , NM_014423.4 , NM_014423.3 , XM_005271963.5
Accession Version	XM_005271963.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	AF4/FMR2 family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005271963.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGAAGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCAGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GTTATCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCAC CATCAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGA 
GGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCCG CACCCAGCAC TTCTCAGTCT 
CAGAAACGGT CCTCAGGCTT ACAGAGTGGA CATAGTAGCC AGCGGACCAG CGCAGGTAGC 
AGTAGTGGCA CTAACAGTAG TGGTCAGAGG CACGACCGTG AGTCATATAA CAATAGTGGG 
AGCAGTAGCC GGAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG TTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AACTCTAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAGGAACA TCAACGCTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAACTCAATC TTTCCCACCC 
TCATTGATGT CAAAGTCCAA TTCAATGTTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GACGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAGC ACTACAGCAG CCAATCCCAT 
GGCAACAGCA TGACTGAGCT GAAGCCCAGC AGCAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CACTGGATGC ATCAGCTTCT GGTGATGTGA GCTGTGTGGA TGAAATCCTA 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAATTT TGGCACTGGA 
GAACAAAAAA GATATAATCC TTCTAAAACT TCAAATGGGC ACCAGTCTAA ATCTATGTTA 
AAAGATGACT TAAAACTAAG CAGCAGTGAA GACAGTGATG GGGAACAGGA TTGTGATAAG 
ACAATGCCGA GGAGTACACC AGGAAGTAAC TCTGAACCTT CACACCATAA TAGTGAAGGA 
GCAGATAACT CCAGGGATGA TTCTAGTAGC CACAGTGGAT CTGAAAGCAG CTCTGGATCT 
GACTCAGAGA GTGAAAGTAG TTCCAGTGAC AGTGAGGCAA ATGAGCCATC CCAGAGTGCA 
TCTCCCGAGC CTGAACCCCC GCCAACAAAC AAATGGCAAC TTGATAATTG GCTGAATAAA 
GTGAACCCAC ATAAAGTGTC ACCCGCCTCT TCAGTGGACA GTAACATCCC ATCATCTCAA 
GGCTACAAAA AGGAAGGCCG AGAGCAGGGC ACTGGGAATA GCTACACTGA TACAAGTGGA 
CCTAAAGAAA CGAGTTCCGC TACTCCGGGA CGAGACTCCA AAACCATCCA AAAGGGATCA 
GAAAGTGGGC GTGGGAGGCA GAAATCTCCT GCACAGAGTG ACAGCACAAC ACAGAGAAGA 
ACTGTAGGCA AAAAACAACC CAAAAAGGCT GAGAAGGCAG CTGCTGAAGA GCCTCGTGGA 
GGCCTGAAGA TAGAAAGTGA AACCCCTGTA GACTTGGCTA GCAGCATGCC CTCCAGCAGA 
CACAAAGCAG CCACCAAAGG CTCAAGGAAA CCCAATATAA AGAAGGAGTC TAAGTCTTCC 
CCTCGACCTA CAGCAGAGAA AAAGAAATAT AAGTCAACAA GTAAATCTTC CCAGAAATCA 
AGGGAAATCA TAGAAACAGA TACCTCATCC TCAGATTCAG ATGAAAGTGA GAGCCTTCCT 
CCTTCCTCAC AAACTCCTAA GTACCCCGAG AGCAATAGGA CTCCTGTTAA ACCCTCCTCA 
GTGGAGGAAG AAGATAGCTT TTTTCGGCAA CGAATGTTCT CTCCTATGGA AGAGAAGGAA 
CTTCTTTCAC CCCTCAGTGA GCCTGATGAC AGGTACCCAC TTATTGTGAA GATTGACCTG 
AATCTTTTGA CTAGAATACC AGGAAAGCCT TACAAAGAAA CAGAGCCGCC CAAGGGGGAA 
AAGAAAAATG TGCCAGAAAA GCACACGAGA GAGGCTCAGA AACAAGCCTC AGAAAAAGTT 
TCCAACAAAG GCAAGAGGAA GCATAAGAAT GAAGATGATA ACCGAGCCAG TGAGAGCAAG 
AAACCCAAAA CGGAGGACAA GAATTCAGCA GGCCATAAGC CATCCAGCAA CAGAGAGTCA 
TCTAAGCAGA GTGCTGCAAA AGAAAAGGAT TTGTTGCCTT CTCCCGCTGG GCCTGTTCCT 
TCAAAAGATC CAAAAACAGA GCATGGCTCT CGGAAGAGGA CTATTAGTCA GTCTTCTTCC 
TTAAAGTCAA GCAGTAACAG CAACAAGGAG ACGAGTGGCA GCAGCAAAAA CAGTTCCTCC 
ACATCAAAGC AGAAGAAGAC CGAAGGGAAG ACTTCCAGTA GCTCCAAGGA GGTTAAGGAA 
AAGGCTCCAA GTAGCTCCTC TAACTGTCCT CCATCTGCAC CAACTCTTGA TTCTTCTAAG 
CCTCGGAGAA CAAAGCTTGT CTTTGATGAC AGAAATTATT CAGCAGACCA TTATTTACAA 
GAAGCAAAAA AGCTAAAGCA CAATGCAGAT GCATTGTCTG ATAGGTTTGA GAAAGCTGTA 
TACTATCTTG ATGCTGTGGT ATCTTTCATT GAATGTGGGA ATGCATTAGA GAAGAATGCT 
CAGGAATCCA AATCCCCATT CCCTATGTAT TCAGAGACGG TGGATCTCAT CAAATACACT 
ATGAAGCTAA AGAATTACTT GGCACCAGAT GCTACAGCTG CAGATAAACG ACTCACAGTA 
CTTTGCCTGC GATGCGAGTC TTTGCTGTAC CTGAGGCTGT TCAAACTGAA GAAGGAAAAT 
GCTCTGAAGT ACTCAAAGAC ACTGACAGAG CACCTGAAGA ATTCTTATAA TAATTCTCAA 
GCACCATCGC CTGGCTTGGG AAGCAAAGCT GTGGGGATGC CTTCCCCTGT TTCTCCAAAG 
CTGTCACCAG GCAATTCAGG AAATTATTCA TCTGGGGCCA GTAGTGCTTC TGCAAGTGGT 
TCTTCAGTGA CCATTCCACA GAAGATCCAC CAGATGGCAG CCAGCTATGT TCAGGTCACA 
TCCAACTTCC TCTATGCCAC CGAAATTTGG GACCAAGCTG AACAGCTTTC CAAAGAGCAA 
AAAGAATTCT TTGCTGAACT GGATAAAGTA ATGGGCCCTC TCATCTTTAA TGCAAGCATC 
ATGACAGATC TAGTTCGTTA TACCCGGCAG GGACTGCACT GGCTTCGCCA GGATGCCAAG 
TTGATATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272020.1
CDS	473..3964
Translation	MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQKRYNPSKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDTSGPKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKAEKAAAEEPRGGLKIESETPVDLASSMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRASESKKPKTEDKNSAGHKPSSNRESSKQSAAKEKDLLPSPAGPVPSKDPKTEHGSRKRTISQSSSLKSSSNSNKETSGSSKNSSSTSKQKKTEGKTSSSSKEVKEKAPSSSSNCPPSAPTLDSSKPRRTKLVFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSETVDLIKYTMKLKNYLAPDATAADKRLTVLCLRCESLLYLRLFKLKKENALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	XM_005271963.4
Organism	Homo sapiens(human)
Definition	Homo sapiens AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271963.4

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGAAGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCAGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GTTATCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCAC CATCAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGA 
GGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCCG CACCCAGCAC TTCTCAGTCT 
CAGAAACGGT CCTCAGGCTT ACAGAGTGGA CATAGTAGCC AGCGGACCAG CGCAGGTAGC 
AGTAGTGGCA CTAACAGTAG TGGTCAGAGG CACGACCGTG AGTCATATAA CAATAGTGGG 
AGCAGTAGCC GGAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG TTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AACTCTAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAGGAACA TCAACGCTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAACTCAATC TTTCCCACCC 
TCATTGATGT CAAAGTCCAA TTCAATGTTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GACGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAGC ACTACAGCAG CCAATCCCAT 
GGCAACAGCA TGACTGAGCT GAAGCCCAGC AGCAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CACTGGATGC ATCAGCTTCT GGTGATGTGA GCTGTGTGGA TGAAATCCTA 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAATTT TGGCACTGGA 
GAACAAAAAA GATATAATCC TTCTAAAACT TCAAATGGGC ACCAGTCTAA ATCTATGTTA 
AAAGATGACT TAAAACTAAG CAGCAGTGAA GACAGTGATG GGGAACAGGA TTGTGATAAG 
ACAATGCCGA GGAGTACACC AGGAAGTAAC TCTGAACCTT CACACCATAA TAGTGAAGGA 
GCAGATAACT CCAGGGATGA TTCTAGTAGC CACAGTGGAT CTGAAAGCAG CTCTGGATCT 
GACTCAGAGA GTGAAAGTAG TTCCAGTGAC AGTGAGGCAA ATGAGCCATC CCAGAGTGCA 
TCTCCCGAGC CTGAACCCCC GCCAACAAAC AAATGGCAAC TTGATAATTG GCTGAATAAA 
GTGAACCCAC ATAAAGTGTC ACCCGCCTCT TCAGTGGACA GTAACATCCC ATCATCTCAA 
GGCTACAAAA AGGAAGGCCG AGAGCAGGGC ACTGGGAATA GCTACACTGA TACAAGTGGA 
CCTAAAGAAA CGAGTTCCGC TACTCCGGGA CGAGACTCCA AAACCATCCA AAAGGGATCA 
GAAAGTGGGC GTGGGAGGCA GAAATCTCCT GCACAGAGTG ACAGCACAAC ACAGAGAAGA 
ACTGTAGGCA AAAAACAACC CAAAAAGGCT GAGAAGGCAG CTGCTGAAGA GCCTCGTGGA 
GGCCTGAAGA TAGAAAGTGA AACCCCTGTA GACTTGGCTA GCAGCATGCC CTCCAGCAGA 
CACAAAGCAG CCACCAAAGG CTCAAGGAAA CCCAATATAA AGAAGGAGTC TAAGTCTTCC 
CCTCGACCTA CAGCAGAGAA AAAGAAATAT AAGTCAACAA GTAAATCTTC CCAGAAATCA 
AGGGAAATCA TAGAAACAGA TACCTCATCC TCAGATTCAG ATGAAAGTGA GAGCCTTCCT 
CCTTCCTCAC AAACTCCTAA GTACCCCGAG AGCAATAGGA CTCCTGTTAA ACCCTCCTCA 
GTGGAGGAAG AAGATAGCTT TTTTCGGCAA CGAATGTTCT CTCCTATGGA AGAGAAGGAA 
CTTCTTTCAC CCCTCAGTGA GCCTGATGAC AGGTACCCAC TTATTGTGAA GATTGACCTG 
AATCTTTTGA CTAGAATACC AGGAAAGCCT TACAAAGAAA CAGAGCCGCC CAAGGGGGAA 
AAGAAAAATG TGCCAGAAAA GCACACGAGA GAGGCTCAGA AACAAGCCTC AGAAAAAGTT 
TCCAACAAAG GCAAGAGGAA GCATAAGAAT GAAGATGATA ACCGAGCCAG TGAGAGCAAG 
AAACCCAAAA CGGAGGACAA GAATTCAGCA GGCCATAAGC CATCCAGCAA CAGAGAGTCA 
TCTAAGCAGA GTGCTGCAAA AGAAAAGGAT TTGTTGCCTT CTCCCGCTGG GCCTGTTCCT 
TCAAAAGATC CAAAAACAGA GCATGGCTCT CGGAAGAGGA CTATTAGTCA GTCTTCTTCC 
TTAAAGTCAA GCAGTAACAG CAACAAGGAG ACGAGTGGCA GCAGCAAAAA CAGTTCCTCC 
ACATCAAAGC AGAAGAAGAC CGAAGGGAAG ACTTCCAGTA GCTCCAAGGA GGTTAAGGAA 
AAGGCTCCAA GTAGCTCCTC TAACTGTCCT CCATCTGCAC CAACTCTTGA TTCTTCTAAG 
CCTCGGAGAA CAAAGCTTGT CTTTGATGAC AGAAATTATT CAGCAGACCA TTATTTACAA 
GAAGCAAAAA AGCTAAAGCA CAATGCAGAT GCATTGTCTG ATAGGTTTGA GAAAGCTGTA 
TACTATCTTG ATGCTGTGGT ATCTTTCATT GAATGTGGGA ATGCATTAGA GAAGAATGCT 
CAGGAATCCA AATCCCCATT CCCTATGTAT TCAGAGACGG TGGATCTCAT CAAATACACT 
ATGAAGCTAA AGAATTACTT GGCACCAGAT GCTACAGCTG CAGATAAACG ACTCACAGTA 
CTTTGCCTGC GATGCGAGTC TTTGCTGTAC CTGAGGCTGT TCAAACTGAA GAAGGAAAAT 
GCTCTGAAGT ACTCAAAGAC ACTGACAGAG CACCTGAAGA ATTCTTATAA TAATTCTCAA 
GCACCATCGC CTGGCTTGGG AAGCAAAGCT GTGGGGATGC CTTCCCCTGT TTCTCCAAAG 
CTGTCACCAG GCAATTCAGG AAATTATTCA TCTGGGGCCA GTAGTGCTTC TGCAAGTGGT 
TCTTCAGTGA CCATTCCACA GAAGATCCAC CAGATGGCAG CCAGCTATGT TCAGGTCACA 
TCCAACTTCC TCTATGCCAC CGAAATTTGG GACCAAGCTG AACAGCTTTC CAAAGAGCAA 
AAAGAATTCT TTGCTGAACT GGATAAAGTA ATGGGCCCTC TCATCTTTAA TGCAAGCATC 
ATGACAGATC TAGTTCGTTA TACCCGGCAG GGACTGCACT GGCTTCGCCA GGATGCCAAG 
TTGATATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_005271963.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272020.1 Homo sapiens AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.

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CloneID	OHu18943
Clone ID Related Accession (Same CDS sequence)	XM_005271963.4 , NM_014423.4 , NM_014423.3 , XM_005271963.5
Accession Version	XM_005271963.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	AF4/FMR2 family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005271963.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGAAGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCAGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GTTATCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCAC CATCAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGA 
GGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCCG CACCCAGCAC TTCTCAGTCT 
CAGAAACGGT CCTCAGGCTT ACAGAGTGGA CATAGTAGCC AGCGGACCAG CGCAGGTAGC 
AGTAGTGGCA CTAACAGTAG TGGTCAGAGG CACGACCGTG AGTCATATAA CAATAGTGGG 
AGCAGTAGCC GGAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG TTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AACTCTAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAGGAACA TCAACGCTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAACTCAATC TTTCCCACCC 
TCATTGATGT CAAAGTCCAA TTCAATGTTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GACGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAGC ACTACAGCAG CCAATCCCAT 
GGCAACAGCA TGACTGAGCT GAAGCCCAGC AGCAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CACTGGATGC ATCAGCTTCT GGTGATGTGA GCTGTGTGGA TGAAATCCTA 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAATTT TGGCACTGGA 
GAACAAAAAA GATATAATCC TTCTAAAACT TCAAATGGGC ACCAGTCTAA ATCTATGTTA 
AAAGATGACT TAAAACTAAG CAGCAGTGAA GACAGTGATG GGGAACAGGA TTGTGATAAG 
ACAATGCCGA GGAGTACACC AGGAAGTAAC TCTGAACCTT CACACCATAA TAGTGAAGGA 
GCAGATAACT CCAGGGATGA TTCTAGTAGC CACAGTGGAT CTGAAAGCAG CTCTGGATCT 
GACTCAGAGA GTGAAAGTAG TTCCAGTGAC AGTGAGGCAA ATGAGCCATC CCAGAGTGCA 
TCTCCCGAGC CTGAACCCCC GCCAACAAAC AAATGGCAAC TTGATAATTG GCTGAATAAA 
GTGAACCCAC ATAAAGTGTC ACCCGCCTCT TCAGTGGACA GTAACATCCC ATCATCTCAA 
GGCTACAAAA AGGAAGGCCG AGAGCAGGGC ACTGGGAATA GCTACACTGA TACAAGTGGA 
CCTAAAGAAA CGAGTTCCGC TACTCCGGGA CGAGACTCCA AAACCATCCA AAAGGGATCA 
GAAAGTGGGC GTGGGAGGCA GAAATCTCCT GCACAGAGTG ACAGCACAAC ACAGAGAAGA 
ACTGTAGGCA AAAAACAACC CAAAAAGGCT GAGAAGGCAG CTGCTGAAGA GCCTCGTGGA 
GGCCTGAAGA TAGAAAGTGA AACCCCTGTA GACTTGGCTA GCAGCATGCC CTCCAGCAGA 
CACAAAGCAG CCACCAAAGG CTCAAGGAAA CCCAATATAA AGAAGGAGTC TAAGTCTTCC 
CCTCGACCTA CAGCAGAGAA AAAGAAATAT AAGTCAACAA GTAAATCTTC CCAGAAATCA 
AGGGAAATCA TAGAAACAGA TACCTCATCC TCAGATTCAG ATGAAAGTGA GAGCCTTCCT 
CCTTCCTCAC AAACTCCTAA GTACCCCGAG AGCAATAGGA CTCCTGTTAA ACCCTCCTCA 
GTGGAGGAAG AAGATAGCTT TTTTCGGCAA CGAATGTTCT CTCCTATGGA AGAGAAGGAA 
CTTCTTTCAC CCCTCAGTGA GCCTGATGAC AGGTACCCAC TTATTGTGAA GATTGACCTG 
AATCTTTTGA CTAGAATACC AGGAAAGCCT TACAAAGAAA CAGAGCCGCC CAAGGGGGAA 
AAGAAAAATG TGCCAGAAAA GCACACGAGA GAGGCTCAGA AACAAGCCTC AGAAAAAGTT 
TCCAACAAAG GCAAGAGGAA GCATAAGAAT GAAGATGATA ACCGAGCCAG TGAGAGCAAG 
AAACCCAAAA CGGAGGACAA GAATTCAGCA GGCCATAAGC CATCCAGCAA CAGAGAGTCA 
TCTAAGCAGA GTGCTGCAAA AGAAAAGGAT TTGTTGCCTT CTCCCGCTGG GCCTGTTCCT 
TCAAAAGATC CAAAAACAGA GCATGGCTCT CGGAAGAGGA CTATTAGTCA GTCTTCTTCC 
TTAAAGTCAA GCAGTAACAG CAACAAGGAG ACGAGTGGCA GCAGCAAAAA CAGTTCCTCC 
ACATCAAAGC AGAAGAAGAC CGAAGGGAAG ACTTCCAGTA GCTCCAAGGA GGTTAAGGAA 
AAGGCTCCAA GTAGCTCCTC TAACTGTCCT CCATCTGCAC CAACTCTTGA TTCTTCTAAG 
CCTCGGAGAA CAAAGCTTGT CTTTGATGAC AGAAATTATT CAGCAGACCA TTATTTACAA 
GAAGCAAAAA AGCTAAAGCA CAATGCAGAT GCATTGTCTG ATAGGTTTGA GAAAGCTGTA 
TACTATCTTG ATGCTGTGGT ATCTTTCATT GAATGTGGGA ATGCATTAGA GAAGAATGCT 
CAGGAATCCA AATCCCCATT CCCTATGTAT TCAGAGACGG TGGATCTCAT CAAATACACT 
ATGAAGCTAA AGAATTACTT GGCACCAGAT GCTACAGCTG CAGATAAACG ACTCACAGTA 
CTTTGCCTGC GATGCGAGTC TTTGCTGTAC CTGAGGCTGT TCAAACTGAA GAAGGAAAAT 
GCTCTGAAGT ACTCAAAGAC ACTGACAGAG CACCTGAAGA ATTCTTATAA TAATTCTCAA 
GCACCATCGC CTGGCTTGGG AAGCAAAGCT GTGGGGATGC CTTCCCCTGT TTCTCCAAAG 
CTGTCACCAG GCAATTCAGG AAATTATTCA TCTGGGGCCA GTAGTGCTTC TGCAAGTGGT 
TCTTCAGTGA CCATTCCACA GAAGATCCAC CAGATGGCAG CCAGCTATGT TCAGGTCACA 
TCCAACTTCC TCTATGCCAC CGAAATTTGG GACCAAGCTG AACAGCTTTC CAAAGAGCAA 
AAAGAATTCT TTGCTGAACT GGATAAAGTA ATGGGCCCTC TCATCTTTAA TGCAAGCATC 
ATGACAGATC TAGTTCGTTA TACCCGGCAG GGACTGCACT GGCTTCGCCA GGATGCCAAG 
TTGATATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272020.1
CDS	182..3673
Translation	MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQKRYNPSKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDTSGPKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKAEKAAAEEPRGGLKIESETPVDLASSMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRASESKKPKTEDKNSAGHKPSSNRESSKQSAAKEKDLLPSPAGPVPSKDPKTEHGSRKRTISQSSSLKSSSNSNKETSGSSKNSSSTSKQKKTEGKTSSSSKEVKEKAPSSSSNCPPSAPTLDSSKPRRTKLVFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSETVDLIKYTMKLKNYLAPDATAADKRLTVLCLRCESLLYLRLFKLKKENALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	XM_005271963.5
Organism	Homo sapiens(human)
Definition	Homo sapiens AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271963.5

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGAAGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCAGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GTTATCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCAC CATCAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGA 
GGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCCG CACCCAGCAC TTCTCAGTCT 
CAGAAACGGT CCTCAGGCTT ACAGAGTGGA CATAGTAGCC AGCGGACCAG CGCAGGTAGC 
AGTAGTGGCA CTAACAGTAG TGGTCAGAGG CACGACCGTG AGTCATATAA CAATAGTGGG 
AGCAGTAGCC GGAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG TTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AACTCTAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAGGAACA TCAACGCTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAACTCAATC TTTCCCACCC 
TCATTGATGT CAAAGTCCAA TTCAATGTTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GACGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAGC ACTACAGCAG CCAATCCCAT 
GGCAACAGCA TGACTGAGCT GAAGCCCAGC AGCAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CACTGGATGC ATCAGCTTCT GGTGATGTGA GCTGTGTGGA TGAAATCCTA 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAATTT TGGCACTGGA 
GAACAAAAAA GATATAATCC TTCTAAAACT TCAAATGGGC ACCAGTCTAA ATCTATGTTA 
AAAGATGACT TAAAACTAAG CAGCAGTGAA GACAGTGATG GGGAACAGGA TTGTGATAAG 
ACAATGCCGA GGAGTACACC AGGAAGTAAC TCTGAACCTT CACACCATAA TAGTGAAGGA 
GCAGATAACT CCAGGGATGA TTCTAGTAGC CACAGTGGAT CTGAAAGCAG CTCTGGATCT 
GACTCAGAGA GTGAAAGTAG TTCCAGTGAC AGTGAGGCAA ATGAGCCATC CCAGAGTGCA 
TCTCCCGAGC CTGAACCCCC GCCAACAAAC AAATGGCAAC TTGATAATTG GCTGAATAAA 
GTGAACCCAC ATAAAGTGTC ACCCGCCTCT TCAGTGGACA GTAACATCCC ATCATCTCAA 
GGCTACAAAA AGGAAGGCCG AGAGCAGGGC ACTGGGAATA GCTACACTGA TACAAGTGGA 
CCTAAAGAAA CGAGTTCCGC TACTCCGGGA CGAGACTCCA AAACCATCCA AAAGGGATCA 
GAAAGTGGGC GTGGGAGGCA GAAATCTCCT GCACAGAGTG ACAGCACAAC ACAGAGAAGA 
ACTGTAGGCA AAAAACAACC CAAAAAGGCT GAGAAGGCAG CTGCTGAAGA GCCTCGTGGA 
GGCCTGAAGA TAGAAAGTGA AACCCCTGTA GACTTGGCTA GCAGCATGCC CTCCAGCAGA 
CACAAAGCAG CCACCAAAGG CTCAAGGAAA CCCAATATAA AGAAGGAGTC TAAGTCTTCC 
CCTCGACCTA CAGCAGAGAA AAAGAAATAT AAGTCAACAA GTAAATCTTC CCAGAAATCA 
AGGGAAATCA TAGAAACAGA TACCTCATCC TCAGATTCAG ATGAAAGTGA GAGCCTTCCT 
CCTTCCTCAC AAACTCCTAA GTACCCCGAG AGCAATAGGA CTCCTGTTAA ACCCTCCTCA 
GTGGAGGAAG AAGATAGCTT TTTTCGGCAA CGAATGTTCT CTCCTATGGA AGAGAAGGAA 
CTTCTTTCAC CCCTCAGTGA GCCTGATGAC AGGTACCCAC TTATTGTGAA GATTGACCTG 
AATCTTTTGA CTAGAATACC AGGAAAGCCT TACAAAGAAA CAGAGCCGCC CAAGGGGGAA 
AAGAAAAATG TGCCAGAAAA GCACACGAGA GAGGCTCAGA AACAAGCCTC AGAAAAAGTT 
TCCAACAAAG GCAAGAGGAA GCATAAGAAT GAAGATGATA ACCGAGCCAG TGAGAGCAAG 
AAACCCAAAA CGGAGGACAA GAATTCAGCA GGCCATAAGC CATCCAGCAA CAGAGAGTCA 
TCTAAGCAGA GTGCTGCAAA AGAAAAGGAT TTGTTGCCTT CTCCCGCTGG GCCTGTTCCT 
TCAAAAGATC CAAAAACAGA GCATGGCTCT CGGAAGAGGA CTATTAGTCA GTCTTCTTCC 
TTAAAGTCAA GCAGTAACAG CAACAAGGAG ACGAGTGGCA GCAGCAAAAA CAGTTCCTCC 
ACATCAAAGC AGAAGAAGAC CGAAGGGAAG ACTTCCAGTA GCTCCAAGGA GGTTAAGGAA 
AAGGCTCCAA GTAGCTCCTC TAACTGTCCT CCATCTGCAC CAACTCTTGA TTCTTCTAAG 
CCTCGGAGAA CAAAGCTTGT CTTTGATGAC AGAAATTATT CAGCAGACCA TTATTTACAA 
GAAGCAAAAA AGCTAAAGCA CAATGCAGAT GCATTGTCTG ATAGGTTTGA GAAAGCTGTA 
TACTATCTTG ATGCTGTGGT ATCTTTCATT GAATGTGGGA ATGCATTAGA GAAGAATGCT 
CAGGAATCCA AATCCCCATT CCCTATGTAT TCAGAGACGG TGGATCTCAT CAAATACACT 
ATGAAGCTAA AGAATTACTT GGCACCAGAT GCTACAGCTG CAGATAAACG ACTCACAGTA 
CTTTGCCTGC GATGCGAGTC TTTGCTGTAC CTGAGGCTGT TCAAACTGAA GAAGGAAAAT 
GCTCTGAAGT ACTCAAAGAC ACTGACAGAG CACCTGAAGA ATTCTTATAA TAATTCTCAA 
GCACCATCGC CTGGCTTGGG AAGCAAAGCT GTGGGGATGC CTTCCCCTGT TTCTCCAAAG 
CTGTCACCAG GCAATTCAGG AAATTATTCA TCTGGGGCCA GTAGTGCTTC TGCAAGTGGT 
TCTTCAGTGA CCATTCCACA GAAGATCCAC CAGATGGCAG CCAGCTATGT TCAGGTCACA 
TCCAACTTCC TCTATGCCAC CGAAATTTGG GACCAAGCTG AACAGCTTTC CAAAGAGCAA 
AAAGAATTCT TTGCTGAACT GGATAAAGTA ATGGGCCCTC TCATCTTTAA TGCAAGCATC 
ATGACAGATC TAGTTCGTTA TACCCGGCAG GGACTGCACT GGCTTCGCCA GGATGCCAAG 
TTGATATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_006714587.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714650.1 Homo sapiens AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.

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CloneID	OHu39544
Clone ID Related Accession (Same CDS sequence)	XM_006714587.3 , XM_006714587.4
Accession Version	XM_006714587.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	AF4/FMR2 family member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714587.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGAAGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCAGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GTTATCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCAC CATCAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGA 
GGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCCG CACCCAGCAC TTCTCAGTCT 
CAGAAACGGT CCTCAGGCTT ACAGAGTGGA CATAGTAGCC AGCGGACCAG CGCAGGTAGC 
AGTAGTGGCA CTAACAGTAG TGGTCAGAGG CACGACCGTG AGTCATATAA CAATAGTGGG 
AGCAGTAGCC GGAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG TTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AACTCTAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAGGAACA TCAACGCTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAACTCAATC TTTCCCACCC 
TCATTGATGT CAAAGTCCAA TTCAATGTTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GACGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAGC ACTACAGCAG CCAATCCCAT 
GGCAACAGCA TGACTGAGCT GAAGCCCAGC AGCAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CACTGGATGC ATCAGCTTCT GGTGATGTGA GCTGTGTGGA TGAAATCCTA 
AAAGAGTCTC AGCAGTCCAA TTTTGGCACT GGAGAACAAA AAAGATATAA TCCTTCTAAA 
ACTTCAAATG GGCACCAGTC TAAATCTATG TTAAAAGATG ACTTAAAACT AAGCAGCAGT 
GAAGACAGTG ATGGGGAACA GGATTGTGAT AAGACAATGC CGAGGAGTAC ACCAGGAAGT 
AACTCTGAAC CTTCACACCA TAATAGTGAA GGAGCAGATA ACTCCAGGGA TGATTCTAGT 
AGCCACAGTG GATCTGAAAG CAGCTCTGGA TCTGACTCAG AGAGTGAAAG TAGTTCCAGT 
GACAGTGAGG CAAATGAGCC ATCCCAGAGT GCATCTCCCG AGCCTGAACC CCCGCCAACA 
AACAAATGGC AACTTGATAA TTGGCTGAAT AAAGTGAACC CACATAAAGT GTCACCCGCC 
TCTTCAGTGG ACAGTAACAT CCCATCATCT CAAGGCTACA AAAAGGAAGG CCGAGAGCAG 
GGCACTGGGA ATAGCTACAC TGATACAAGT GGACCTAAAG AAACGAGTTC CGCTACTCCG 
GGACGAGACT CCAAAACCAT CCAAAAGGGA TCAGAAAGTG GGCGTGGGAG GCAGAAATCT 
CCTGCACAGA GTGACAGCAC AACACAGAGA AGAACTGTAG GCAAAAAACA ACCCAAAAAG 
GCTGAGAAGG CAGCTGCTGA AGAGCCTCGT GGAGGCCTGA AGATAGAAAG TGAAACCCCT 
GTAGACTTGG CTAGCAGCAT GCCCTCCAGC AGACACAAAG CAGCCACCAA AGGCTCAAGG 
AAACCCAATA TAAAGAAGGA GTCTAAGTCT TCCCCTCGAC CTACAGCAGA GAAAAAGAAA 
TATAAGTCAA CAAGTAAATC TTCCCAGAAA TCAAGGGAAA TCATAGAAAC AGATACCTCA 
TCCTCAGATT CAGATGAAAG TGAGAGCCTT CCTCCTTCCT CACAAACTCC TAAGTACCCC 
GAGAGCAATA GGACTCCTGT TAAACCCTCC TCAGTGGAGG AAGAAGATAG CTTTTTTCGG 
CAACGAATGT TCTCTCCTAT GGAAGAGAAG GAACTTCTTT CACCCCTCAG TGAGCCTGAT 
GACAGGTACC CACTTATTGT GAAGATTGAC CTGAATCTTT TGACTAGAAT ACCAGGAAAG 
CCTTACAAAG AAACAGAGCC GCCCAAGGGG GAAAAGAAAA ATGTGCCAGA AAAGCACACG 
AGAGAGGCTC AGAAACAAGC CTCAGAAAAA GTTTCCAACA AAGGCAAGAG GAAGCATAAG 
AATGAAGATG ATAACCGAGC CAGTGAGAGC AAGAAACCCA AAACGGAGGA CAAGAATTCA 
GCAGGCCATA AGCCATCCAG CAACAGAGAG TCATCTAAGC AGAGTGCTGC AAAAGAAAAG 
GATTTGTTGC CTTCTCCCGC TGGGCCTGTT CCTTCAAAAG ATCCAAAAAC AGAGCATGGC 
TCTCGGAAGA GGACTATTAG TCAGTCTTCT TCCTTAAAGT CAAGCAGTAA CAGCAACAAG 
GAGACGAGTG GCAGCAGCAA AAACAGTTCC TCCACATCAA AGCAGAAGAA GACCGAAGGG 
AAGACTTCCA GTAGCTCCAA GGAGGTTAAG GAAAAGGCTC CAAGTAGCTC CTCTAACTGT 
CCTCCATCTG CACCAACTCT TGATTCTTCT AAGCCTCGGA GAACAAAGCT TGTCTTTGAT 
GACAGAAATT ATTCAGCAGA CCATTATTTA CAAGAAGCAA AAAAGCTAAA GCACAATGCA 
GATGCATTGT CTGATAGGTT TGAGAAAGCT GTATACTATC TTGATGCTGT GGTATCTTTC 
ATTGAATGTG GGAATGCATT AGAGAAGAAT GCTCAGGAAT CCAAATCCCC ATTCCCTATG 
TATTCAGAGA CGGTGGATCT CATCAAATAC ACTATGAAGC TAAAGAATTA CTTGGCACCA 
GATGCTACAG CTGCAGATAA ACGACTCACA GTACTTTGCC TGCGATGCGA GTCTTTGCTG 
TACCTGAGGC TGTTCAAACT GAAGAAGGAA AATGCTCTGA AGTACTCAAA GACACTGACA 
GAGCACCTGA AGAATTCTTA TAATAATTCT CAAGCACCAT CGCCTGGCTT GGGAAGCAAA 
GCTGTGGGGA TGCCTTCCCC TGTTTCTCCA AAGCTGTCAC CAGGCAATTC AGGAAATTAT 
TCATCTGGGG CCAGTAGTGC TTCTGCAAGT GGTTCTTCAG TGACCATTCC ACAGAAGATC 
CACCAGATGG CAGCCAGCTA TGTTCAGGTC ACATCCAACT TCCTCTATGC CACCGAAATT 
TGGGACCAAG CTGAACAGCT TTCCAAAGAG CAAAAAGAAT TCTTTGCTGA ACTGGATAAA 
GTAATGGGCC CTCTCATCTT TAATGCAAGC ATCATGACAG ATCTAGTTCG TTATACCCGG 
CAGGGACTGC ACTGGCTTCG CCAGGATGCC AAGTTGATAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714650.1
CDS	409..3813
Translation	MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKESQQSNFGTGEQKRYNPSKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDTSGPKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKAEKAAAEEPRGGLKIESETPVDLASSMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRASESKKPKTEDKNSAGHKPSSNRESSKQSAAKEKDLLPSPAGPVPSKDPKTEHGSRKRTISQSSSLKSSSNSNKETSGSSKNSSSTSKQKKTEGKTSSSSKEVKEKAPSSSSNCPPSAPTLDSSKPRRTKLVFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSETVDLIKYTMKLKNYLAPDATAADKRLTVLCLRCESLLYLRLFKLKKENALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	XM_006714587.3
Organism	Homo sapiens(human)
Definition	Homo sapiens AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714587.3

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGAAGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCAGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GTTATCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCAC CATCAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGA 
GGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCCG CACCCAGCAC TTCTCAGTCT 
CAGAAACGGT CCTCAGGCTT ACAGAGTGGA CATAGTAGCC AGCGGACCAG CGCAGGTAGC 
AGTAGTGGCA CTAACAGTAG TGGTCAGAGG CACGACCGTG AGTCATATAA CAATAGTGGG 
AGCAGTAGCC GGAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG TTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AACTCTAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAGGAACA TCAACGCTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAACTCAATC TTTCCCACCC 
TCATTGATGT CAAAGTCCAA TTCAATGTTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GACGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAGC ACTACAGCAG CCAATCCCAT 
GGCAACAGCA TGACTGAGCT GAAGCCCAGC AGCAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CACTGGATGC ATCAGCTTCT GGTGATGTGA GCTGTGTGGA TGAAATCCTA 
AAAGAGTCTC AGCAGTCCAA TTTTGGCACT GGAGAACAAA AAAGATATAA TCCTTCTAAA 
ACTTCAAATG GGCACCAGTC TAAATCTATG TTAAAAGATG ACTTAAAACT AAGCAGCAGT 
GAAGACAGTG ATGGGGAACA GGATTGTGAT AAGACAATGC CGAGGAGTAC ACCAGGAAGT 
AACTCTGAAC CTTCACACCA TAATAGTGAA GGAGCAGATA ACTCCAGGGA TGATTCTAGT 
AGCCACAGTG GATCTGAAAG CAGCTCTGGA TCTGACTCAG AGAGTGAAAG TAGTTCCAGT 
GACAGTGAGG CAAATGAGCC ATCCCAGAGT GCATCTCCCG AGCCTGAACC CCCGCCAACA 
AACAAATGGC AACTTGATAA TTGGCTGAAT AAAGTGAACC CACATAAAGT GTCACCCGCC 
TCTTCAGTGG ACAGTAACAT CCCATCATCT CAAGGCTACA AAAAGGAAGG CCGAGAGCAG 
GGCACTGGGA ATAGCTACAC TGATACAAGT GGACCTAAAG AAACGAGTTC CGCTACTCCG 
GGACGAGACT CCAAAACCAT CCAAAAGGGA TCAGAAAGTG GGCGTGGGAG GCAGAAATCT 
CCTGCACAGA GTGACAGCAC AACACAGAGA AGAACTGTAG GCAAAAAACA ACCCAAAAAG 
GCTGAGAAGG CAGCTGCTGA AGAGCCTCGT GGAGGCCTGA AGATAGAAAG TGAAACCCCT 
GTAGACTTGG CTAGCAGCAT GCCCTCCAGC AGACACAAAG CAGCCACCAA AGGCTCAAGG 
AAACCCAATA TAAAGAAGGA GTCTAAGTCT TCCCCTCGAC CTACAGCAGA GAAAAAGAAA 
TATAAGTCAA CAAGTAAATC TTCCCAGAAA TCAAGGGAAA TCATAGAAAC AGATACCTCA 
TCCTCAGATT CAGATGAAAG TGAGAGCCTT CCTCCTTCCT CACAAACTCC TAAGTACCCC 
GAGAGCAATA GGACTCCTGT TAAACCCTCC TCAGTGGAGG AAGAAGATAG CTTTTTTCGG 
CAACGAATGT TCTCTCCTAT GGAAGAGAAG GAACTTCTTT CACCCCTCAG TGAGCCTGAT 
GACAGGTACC CACTTATTGT GAAGATTGAC CTGAATCTTT TGACTAGAAT ACCAGGAAAG 
CCTTACAAAG AAACAGAGCC GCCCAAGGGG GAAAAGAAAA ATGTGCCAGA AAAGCACACG 
AGAGAGGCTC AGAAACAAGC CTCAGAAAAA GTTTCCAACA AAGGCAAGAG GAAGCATAAG 
AATGAAGATG ATAACCGAGC CAGTGAGAGC AAGAAACCCA AAACGGAGGA CAAGAATTCA 
GCAGGCCATA AGCCATCCAG CAACAGAGAG TCATCTAAGC AGAGTGCTGC AAAAGAAAAG 
GATTTGTTGC CTTCTCCCGC TGGGCCTGTT CCTTCAAAAG ATCCAAAAAC AGAGCATGGC 
TCTCGGAAGA GGACTATTAG TCAGTCTTCT TCCTTAAAGT CAAGCAGTAA CAGCAACAAG 
GAGACGAGTG GCAGCAGCAA AAACAGTTCC TCCACATCAA AGCAGAAGAA GACCGAAGGG 
AAGACTTCCA GTAGCTCCAA GGAGGTTAAG GAAAAGGCTC CAAGTAGCTC CTCTAACTGT 
CCTCCATCTG CACCAACTCT TGATTCTTCT AAGCCTCGGA GAACAAAGCT TGTCTTTGAT 
GACAGAAATT ATTCAGCAGA CCATTATTTA CAAGAAGCAA AAAAGCTAAA GCACAATGCA 
GATGCATTGT CTGATAGGTT TGAGAAAGCT GTATACTATC TTGATGCTGT GGTATCTTTC 
ATTGAATGTG GGAATGCATT AGAGAAGAAT GCTCAGGAAT CCAAATCCCC ATTCCCTATG 
TATTCAGAGA CGGTGGATCT CATCAAATAC ACTATGAAGC TAAAGAATTA CTTGGCACCA 
GATGCTACAG CTGCAGATAA ACGACTCACA GTACTTTGCC TGCGATGCGA GTCTTTGCTG 
TACCTGAGGC TGTTCAAACT GAAGAAGGAA AATGCTCTGA AGTACTCAAA GACACTGACA 
GAGCACCTGA AGAATTCTTA TAATAATTCT CAAGCACCAT CGCCTGGCTT GGGAAGCAAA 
GCTGTGGGGA TGCCTTCCCC TGTTTCTCCA AAGCTGTCAC CAGGCAATTC AGGAAATTAT 
TCATCTGGGG CCAGTAGTGC TTCTGCAAGT GGTTCTTCAG TGACCATTCC ACAGAAGATC 
CACCAGATGG CAGCCAGCTA TGTTCAGGTC ACATCCAACT TCCTCTATGC CACCGAAATT 
TGGGACCAAG CTGAACAGCT TTCCAAAGAG CAAAAAGAAT TCTTTGCTGA ACTGGATAAA 
GTAATGGGCC CTCTCATCTT TAATGCAAGC ATCATGACAG ATCTAGTTCG TTATACCCGG 
CAGGGACTGC ACTGGCTTCG CCAGGATGCC AAGTTGATAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_006714587.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714650.1 Homo sapiens AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu39544
Clone ID Related Accession (Same CDS sequence)	XM_006714587.3 , XM_006714587.4
Accession Version	XM_006714587.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	AF4/FMR2 family member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006714587.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGAAGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCAGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GTTATCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCAC CATCAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGA 
GGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCCG CACCCAGCAC TTCTCAGTCT 
CAGAAACGGT CCTCAGGCTT ACAGAGTGGA CATAGTAGCC AGCGGACCAG CGCAGGTAGC 
AGTAGTGGCA CTAACAGTAG TGGTCAGAGG CACGACCGTG AGTCATATAA CAATAGTGGG 
AGCAGTAGCC GGAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG TTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AACTCTAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAGGAACA TCAACGCTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAACTCAATC TTTCCCACCC 
TCATTGATGT CAAAGTCCAA TTCAATGTTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GACGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAGC ACTACAGCAG CCAATCCCAT 
GGCAACAGCA TGACTGAGCT GAAGCCCAGC AGCAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CACTGGATGC ATCAGCTTCT GGTGATGTGA GCTGTGTGGA TGAAATCCTA 
AAAGAGTCTC AGCAGTCCAA TTTTGGCACT GGAGAACAAA AAAGATATAA TCCTTCTAAA 
ACTTCAAATG GGCACCAGTC TAAATCTATG TTAAAAGATG ACTTAAAACT AAGCAGCAGT 
GAAGACAGTG ATGGGGAACA GGATTGTGAT AAGACAATGC CGAGGAGTAC ACCAGGAAGT 
AACTCTGAAC CTTCACACCA TAATAGTGAA GGAGCAGATA ACTCCAGGGA TGATTCTAGT 
AGCCACAGTG GATCTGAAAG CAGCTCTGGA TCTGACTCAG AGAGTGAAAG TAGTTCCAGT 
GACAGTGAGG CAAATGAGCC ATCCCAGAGT GCATCTCCCG AGCCTGAACC CCCGCCAACA 
AACAAATGGC AACTTGATAA TTGGCTGAAT AAAGTGAACC CACATAAAGT GTCACCCGCC 
TCTTCAGTGG ACAGTAACAT CCCATCATCT CAAGGCTACA AAAAGGAAGG CCGAGAGCAG 
GGCACTGGGA ATAGCTACAC TGATACAAGT GGACCTAAAG AAACGAGTTC CGCTACTCCG 
GGACGAGACT CCAAAACCAT CCAAAAGGGA TCAGAAAGTG GGCGTGGGAG GCAGAAATCT 
CCTGCACAGA GTGACAGCAC AACACAGAGA AGAACTGTAG GCAAAAAACA ACCCAAAAAG 
GCTGAGAAGG CAGCTGCTGA AGAGCCTCGT GGAGGCCTGA AGATAGAAAG TGAAACCCCT 
GTAGACTTGG CTAGCAGCAT GCCCTCCAGC AGACACAAAG CAGCCACCAA AGGCTCAAGG 
AAACCCAATA TAAAGAAGGA GTCTAAGTCT TCCCCTCGAC CTACAGCAGA GAAAAAGAAA 
TATAAGTCAA CAAGTAAATC TTCCCAGAAA TCAAGGGAAA TCATAGAAAC AGATACCTCA 
TCCTCAGATT CAGATGAAAG TGAGAGCCTT CCTCCTTCCT CACAAACTCC TAAGTACCCC 
GAGAGCAATA GGACTCCTGT TAAACCCTCC TCAGTGGAGG AAGAAGATAG CTTTTTTCGG 
CAACGAATGT TCTCTCCTAT GGAAGAGAAG GAACTTCTTT CACCCCTCAG TGAGCCTGAT 
GACAGGTACC CACTTATTGT GAAGATTGAC CTGAATCTTT TGACTAGAAT ACCAGGAAAG 
CCTTACAAAG AAACAGAGCC GCCCAAGGGG GAAAAGAAAA ATGTGCCAGA AAAGCACACG 
AGAGAGGCTC AGAAACAAGC CTCAGAAAAA GTTTCCAACA AAGGCAAGAG GAAGCATAAG 
AATGAAGATG ATAACCGAGC CAGTGAGAGC AAGAAACCCA AAACGGAGGA CAAGAATTCA 
GCAGGCCATA AGCCATCCAG CAACAGAGAG TCATCTAAGC AGAGTGCTGC AAAAGAAAAG 
GATTTGTTGC CTTCTCCCGC TGGGCCTGTT CCTTCAAAAG ATCCAAAAAC AGAGCATGGC 
TCTCGGAAGA GGACTATTAG TCAGTCTTCT TCCTTAAAGT CAAGCAGTAA CAGCAACAAG 
GAGACGAGTG GCAGCAGCAA AAACAGTTCC TCCACATCAA AGCAGAAGAA GACCGAAGGG 
AAGACTTCCA GTAGCTCCAA GGAGGTTAAG GAAAAGGCTC CAAGTAGCTC CTCTAACTGT 
CCTCCATCTG CACCAACTCT TGATTCTTCT AAGCCTCGGA GAACAAAGCT TGTCTTTGAT 
GACAGAAATT ATTCAGCAGA CCATTATTTA CAAGAAGCAA AAAAGCTAAA GCACAATGCA 
GATGCATTGT CTGATAGGTT TGAGAAAGCT GTATACTATC TTGATGCTGT GGTATCTTTC 
ATTGAATGTG GGAATGCATT AGAGAAGAAT GCTCAGGAAT CCAAATCCCC ATTCCCTATG 
TATTCAGAGA CGGTGGATCT CATCAAATAC ACTATGAAGC TAAAGAATTA CTTGGCACCA 
GATGCTACAG CTGCAGATAA ACGACTCACA GTACTTTGCC TGCGATGCGA GTCTTTGCTG 
TACCTGAGGC TGTTCAAACT GAAGAAGGAA AATGCTCTGA AGTACTCAAA GACACTGACA 
GAGCACCTGA AGAATTCTTA TAATAATTCT CAAGCACCAT CGCCTGGCTT GGGAAGCAAA 
GCTGTGGGGA TGCCTTCCCC TGTTTCTCCA AAGCTGTCAC CAGGCAATTC AGGAAATTAT 
TCATCTGGGG CCAGTAGTGC TTCTGCAAGT GGTTCTTCAG TGACCATTCC ACAGAAGATC 
CACCAGATGG CAGCCAGCTA TGTTCAGGTC ACATCCAACT TCCTCTATGC CACCGAAATT 
TGGGACCAAG CTGAACAGCT TTCCAAAGAG CAAAAAGAAT TCTTTGCTGA ACTGGATAAA 
GTAATGGGCC CTCTCATCTT TAATGCAAGC ATCATGACAG ATCTAGTTCG TTATACCCGG 
CAGGGACTGC ACTGGCTTCG CCAGGATGCC AAGTTGATAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714650.1
CDS	402..3806
Translation	MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKESQQSNFGTGEQKRYNPSKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDTSGPKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKAEKAAAEEPRGGLKIESETPVDLASSMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRASESKKPKTEDKNSAGHKPSSNRESSKQSAAKEKDLLPSPAGPVPSKDPKTEHGSRKRTISQSSSLKSSSNSNKETSGSSKNSSSTSKQKKTEGKTSSSSKEVKEKAPSSSSNCPPSAPTLDSSKPRRTKLVFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSETVDLIKYTMKLKNYLAPDATAADKRLTVLCLRCESLLYLRLFKLKKENALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	XM_006714587.4
Organism	Homo sapiens(human)
Definition	Homo sapiens AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714587.4

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGAAGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCAGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GTTATCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCAC CATCAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGA 
GGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCCG CACCCAGCAC TTCTCAGTCT 
CAGAAACGGT CCTCAGGCTT ACAGAGTGGA CATAGTAGCC AGCGGACCAG CGCAGGTAGC 
AGTAGTGGCA CTAACAGTAG TGGTCAGAGG CACGACCGTG AGTCATATAA CAATAGTGGG 
AGCAGTAGCC GGAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG TTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AACTCTAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAGGAACA TCAACGCTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAACTCAATC TTTCCCACCC 
TCATTGATGT CAAAGTCCAA TTCAATGTTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GACGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAGC ACTACAGCAG CCAATCCCAT 
GGCAACAGCA TGACTGAGCT GAAGCCCAGC AGCAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CACTGGATGC ATCAGCTTCT GGTGATGTGA GCTGTGTGGA TGAAATCCTA 
AAAGAGTCTC AGCAGTCCAA TTTTGGCACT GGAGAACAAA AAAGATATAA TCCTTCTAAA 
ACTTCAAATG GGCACCAGTC TAAATCTATG TTAAAAGATG ACTTAAAACT AAGCAGCAGT 
GAAGACAGTG ATGGGGAACA GGATTGTGAT AAGACAATGC CGAGGAGTAC ACCAGGAAGT 
AACTCTGAAC CTTCACACCA TAATAGTGAA GGAGCAGATA ACTCCAGGGA TGATTCTAGT 
AGCCACAGTG GATCTGAAAG CAGCTCTGGA TCTGACTCAG AGAGTGAAAG TAGTTCCAGT 
GACAGTGAGG CAAATGAGCC ATCCCAGAGT GCATCTCCCG AGCCTGAACC CCCGCCAACA 
AACAAATGGC AACTTGATAA TTGGCTGAAT AAAGTGAACC CACATAAAGT GTCACCCGCC 
TCTTCAGTGG ACAGTAACAT CCCATCATCT CAAGGCTACA AAAAGGAAGG CCGAGAGCAG 
GGCACTGGGA ATAGCTACAC TGATACAAGT GGACCTAAAG AAACGAGTTC CGCTACTCCG 
GGACGAGACT CCAAAACCAT CCAAAAGGGA TCAGAAAGTG GGCGTGGGAG GCAGAAATCT 
CCTGCACAGA GTGACAGCAC AACACAGAGA AGAACTGTAG GCAAAAAACA ACCCAAAAAG 
GCTGAGAAGG CAGCTGCTGA AGAGCCTCGT GGAGGCCTGA AGATAGAAAG TGAAACCCCT 
GTAGACTTGG CTAGCAGCAT GCCCTCCAGC AGACACAAAG CAGCCACCAA AGGCTCAAGG 
AAACCCAATA TAAAGAAGGA GTCTAAGTCT TCCCCTCGAC CTACAGCAGA GAAAAAGAAA 
TATAAGTCAA CAAGTAAATC TTCCCAGAAA TCAAGGGAAA TCATAGAAAC AGATACCTCA 
TCCTCAGATT CAGATGAAAG TGAGAGCCTT CCTCCTTCCT CACAAACTCC TAAGTACCCC 
GAGAGCAATA GGACTCCTGT TAAACCCTCC TCAGTGGAGG AAGAAGATAG CTTTTTTCGG 
CAACGAATGT TCTCTCCTAT GGAAGAGAAG GAACTTCTTT CACCCCTCAG TGAGCCTGAT 
GACAGGTACC CACTTATTGT GAAGATTGAC CTGAATCTTT TGACTAGAAT ACCAGGAAAG 
CCTTACAAAG AAACAGAGCC GCCCAAGGGG GAAAAGAAAA ATGTGCCAGA AAAGCACACG 
AGAGAGGCTC AGAAACAAGC CTCAGAAAAA GTTTCCAACA AAGGCAAGAG GAAGCATAAG 
AATGAAGATG ATAACCGAGC CAGTGAGAGC AAGAAACCCA AAACGGAGGA CAAGAATTCA 
GCAGGCCATA AGCCATCCAG CAACAGAGAG TCATCTAAGC AGAGTGCTGC AAAAGAAAAG 
GATTTGTTGC CTTCTCCCGC TGGGCCTGTT CCTTCAAAAG ATCCAAAAAC AGAGCATGGC 
TCTCGGAAGA GGACTATTAG TCAGTCTTCT TCCTTAAAGT CAAGCAGTAA CAGCAACAAG 
GAGACGAGTG GCAGCAGCAA AAACAGTTCC TCCACATCAA AGCAGAAGAA GACCGAAGGG 
AAGACTTCCA GTAGCTCCAA GGAGGTTAAG GAAAAGGCTC CAAGTAGCTC CTCTAACTGT 
CCTCCATCTG CACCAACTCT TGATTCTTCT AAGCCTCGGA GAACAAAGCT TGTCTTTGAT 
GACAGAAATT ATTCAGCAGA CCATTATTTA CAAGAAGCAA AAAAGCTAAA GCACAATGCA 
GATGCATTGT CTGATAGGTT TGAGAAAGCT GTATACTATC TTGATGCTGT GGTATCTTTC 
ATTGAATGTG GGAATGCATT AGAGAAGAAT GCTCAGGAAT CCAAATCCCC ATTCCCTATG 
TATTCAGAGA CGGTGGATCT CATCAAATAC ACTATGAAGC TAAAGAATTA CTTGGCACCA 
GATGCTACAG CTGCAGATAA ACGACTCACA GTACTTTGCC TGCGATGCGA GTCTTTGCTG 
TACCTGAGGC TGTTCAAACT GAAGAAGGAA AATGCTCTGA AGTACTCAAA GACACTGACA 
GAGCACCTGA AGAATTCTTA TAATAATTCT CAAGCACCAT CGCCTGGCTT GGGAAGCAAA 
GCTGTGGGGA TGCCTTCCCC TGTTTCTCCA AAGCTGTCAC CAGGCAATTC AGGAAATTAT 
TCATCTGGGG CCAGTAGTGC TTCTGCAAGT GGTTCTTCAG TGACCATTCC ACAGAAGATC 
CACCAGATGG CAGCCAGCTA TGTTCAGGTC ACATCCAACT TCCTCTATGC CACCGAAATT 
TGGGACCAAG CTGAACAGCT TTCCAAAGAG CAAAAAGAAT TCTTTGCTGA ACTGGATAAA 
GTAATGGGCC CTCTCATCTT TAATGCAAGC ATCATGACAG ATCTAGTTCG TTATACCCGG 
CAGGGACTGC ACTGGCTTCG CCAGGATGCC AAGTTGATAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Structural basis for ELL2 and AFF4 activation of HIV-1 proviral transcription.
Nature communications814076(2017 Jan)
Qi S,Li Z,Schulze-Gahmen U,Stjepanovic G,Zhou Q,Hurley JH

Insights into HIV-1 proviral transcription from integrative structure and dynamics of the Tat:AFF4:P-TEFb:TAR complex.
eLife5(2016 10)
Schulze-Gahmen U,Echeverria I,Stjepanovic G,Bai Y,Lu H,Schneidman-Duhovny D,Doudna JA,Zhou Q,Sali A,Hurley JH

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AFF4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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