AFF4 cDNA ORF clone, Gallus gallus(chicken)

The following AFF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa42141 XM_015294064.2
Latest version!
Gallus gallus AF4/FMR2 family member 4 (AFF4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa14806 XM_004945030.3
Latest version!
Gallus gallus AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa14806 XM_004945031.3
Latest version!
Gallus gallus AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa14806 XM_015294063.2
Latest version!
Gallus gallus AF4/FMR2 family member 4 (AFF4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa14807 XM_004945032.3
Latest version!
Gallus gallus AF4/FMR2 family member 4 (AFF4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa14809 XM_004945033.3
Latest version!
Gallus gallus AF4/FMR2 family member 4 (AFF4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OGa14806 XM_004945031.2 Gallus gallus AF4/FMR2 family, member 4 (AFF4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa14806 XM_015294063.1 Gallus gallus AF4/FMR2 family, member 4 (AFF4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa14809 XM_004945033.2 Gallus gallus AF4/FMR2 family, member 4 (AFF4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa42141 XM_015294064.1 Gallus gallus AF4/FMR2 family, member 4 (AFF4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa14806 XM_004945030.2 Gallus gallus AF4/FMR2 family, member 4 (AFF4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa14807 XM_004945032.2 Gallus gallus AF4/FMR2 family, member 4 (AFF4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa42141
    Clone ID Related Accession (Same CDS sequence) XM_015294064.2 , XM_015294064.1
    Accession Version XM_015294064.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3459bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product AF4/FMR2 family member 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006100.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294064.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGAGAT GACCCATTCT TGGCCTCCTC CGCTGACTGC TATTCATACA
    CCATGCAAAA CTGAACCATC AAAATTTCCT TTTCCAACAA AGGAGTCTCA ACAGTCCAGT
    TTTGGCACTG GAGAACAGAA ACGATATAAT CCTTCATCAA AAACATCCAA TGGCCATCAG
    TCCAAATCAT GTGAATCGAA CCAGACAGAT ATGTTGAAAG ATGACTTAAA ACTTAGCAGT
    AGTGAAGACA GCGATGGAGA GCAGGACTGT GATAAGACAG TGCCAAGGAG CACACCTGGA
    AGTAGTTCTG AACCTTCACA GCATAACAGT GAAGGAGCAG ATAATTCAAG GGATGACTCG
    AGCAGCCACA GTGGGTCTGA AAGCAGTTCA GGATCAGACT CTGAAAGTGA AAGCAGTTCC
    AGTGATAGTG AAGCTAATGA ACCATCACAG AGTGCATCCC CTGAGCCAGA GCCACCGCCA
    ACAAACAAGT GGCAACTGGA TAACTGGTTG AACAAAGTTA ATTCACATAA AGTGTCACCA
    GCTTCTTCCG TGGACAGCAA TATCCCATCC TCACAAGGCT ACAAAAAAGA GGGACGGGAT
    CAGGGAACAG GAAGTGGGTA CACTGATCAA AGTGGATCCA AGGACTCCAT TTCTTCAACA
    CCAGGGCGAG ATTCCAAACC CACCCAAAAG GGCTCAGAGA GCAGTCGTGG CAGGCAGAAA
    TCACCTGCCC AGAGTGACAG CTCAACACAG AGGAGGACTG TGGGTAAAAA GCAGCCCAAG
    AAGGCAGAAA AGACATCTGT TGAAGAGCCT AGAGGAGGTC TTAAAATAGA AAGTGAAACC
    CCAGTAGACA TGGCAACAAA CATACCTTCG AACAGGCATA AGGCAGCCAC TAAAGGTTCC
    AGAAAACCTA ATATAAAGAA AGAGCCCAAA TCTTCCCCTC GGCCTACAAC AGAGAAGAAG
    AAATACAAGG CTTCAAGCAA ATCTGCCCAG AAATCCAGGG AATTCATAGA AACAGATACC
    TCATCTTCTG ATTCAGATGA AAATGAAAGC CTGCCTCCTT CATCACAAAC ACCCAAATAC
    TCCGAGAGCA ATAGGACTCC TGTTAAATCT TCCATGGAGG AGGATGACAG CTTTTTTCGG
    CAAAGAATGT TCTCTCCTAT GGAAGAGAAA GAGCTTCTTT CACCACTCAG CGATCCTGAT
    GAAAGGTACC CACTTATTGT GAAGATTGAC CTGAGCCTCT TATCAAGAAT ACCAGGGAAG
    CCTTACAAGG ATGCTGATGT GCCCAAAGTG GAAAAGAAGA ATGTGCCAGA GAAGCATGCA
    AGAGAATCCC AGAAGCAGCC TTCTGATAAA AGTTCTACGA AAGGCAAAAG GAAACATAAA
    CAGAATGAGG ATGAAAACAG AGCCAGTGAA AGCAAGAAAA CGAAACTGGA AGAAAAAGCT
    CCATCAGGAC ACAAGACTTC TAGCAGCAGG GAGTCTTTAA AACCAAGTGC TGTTAAAGAG
    AAGGATTTAC TGCCATCTCC TGCTGCCCCA GTCCTTCAGA AGGATTCCAA ACAAGAGCAC
    GGGTCCCGGA AGAGGACAGT CAGTCAGTCG TCATCCTTGA AGTCCAGCAG CAATGTTAGT
    AAGGACAGCA GCAGTGGCAG TAAAAGCAGC TCATCTTCTA AACAGAAGAA GATGGAGGGC
    AAAGGTTCTG GCAGTGCTAA AGAAACAAAG GAAAAGATTC TGAACAATTC ATCGAACTGC
    CCTCCTGTTT CTGCTCCATC TACAGAGAGC TCAAAATCAA GAAGAGCAAA ACTTGTTTTT
    GATGACAGGA CTTACTCAGC TGATCATTAT TTACAGGAAG CAAAGAAGTT AAAACATAAT
    GCAGATGCAT TGTCTGACAG GTTTGAGAAG GCTGTGTACT ACCTAGATGC AGTAGTGTCG
    TTCATTGAGT GTGGGAATGC ATTGGAGAAA AATGCTCAGG AATCCAAGTC CCCGTTCCCT
    ATGTATTCAG AAACTGTGGA ATTAATCAAG TACACTATGA AACTGAAGAA TTACTTGGCA
    CCGGATGCTA CGGCTGCAGA CAAAAGGCTA GCAGTGCTTT GTCTTCGGTG CCAATCTCTA
    CTATACCTAA GGCTTTTCAA ACTGAAAAAG GAAAGTGCAT TGAAATATTC TAAAACTCTG
    ACTGAACATC TGAAGAATTC CTATAATAAT TCTCAAGCAC CGTCACCTGG TATGGGAAGC
    AAGCCTGTTG GTATGCCATC TCCAGTCTCT CCAAAGCTGT CTCCAGGGAA TTCAGGAAAT
    TACTCTTCTG GGACAGCCAA CCCTTCAGGG AGTGGGTCTT CGGTGATGAT CCCCCAGAGG
    ATCCATCAGA TGGCAGCCAG CTATGTCCAA GTTACATCCA ACTTCCTCTA TGCCACTGAA
    ATTTGGGACC AAGCTGAACA GCTGTCTAAA GAACAGAAAG AGTTCTTTGC TGAACTGGAT
    AAAGCTATGG GCCCTCTGGT TTTTAATTCA AGCATCATGA CAGACCTAGT GCGTTACACC
    CGTCAGGGAC TACACTGGTT GCGCCTGGAT GCCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149550.1
    CDS1175..4633
    Translation

    Target ORF information:

    RefSeq Version XM_015294064.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus AF4/FMR2 family member 4 (AFF4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294064.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGAGAT GACCCATTCT TGGCCTCCTC CGCTGACTGC TATTCATACA
    CCATGCAAAA CTGAACCATC AAAATTTCCT TTTCCAACAA AGGAGTCTCA ACAGTCCAGT
    TTTGGCACTG GAGAACAGAA ACGATATAAT CCTTCATCAA AAACATCCAA TGGCCATCAG
    TCCAAATCAT GTGAATCGAA CCAGACAGAT ATGTTGAAAG ATGACTTAAA ACTTAGCAGT
    AGTGAAGACA GCGATGGAGA GCAGGACTGT GATAAGACAG TGCCAAGGAG CACACCTGGA
    AGTAGTTCTG AACCTTCACA GCATAACAGT GAAGGAGCAG ATAATTCAAG GGATGACTCG
    AGCAGCCACA GTGGGTCTGA AAGCAGTTCA GGATCAGACT CTGAAAGTGA AAGCAGTTCC
    AGTGATAGTG AAGCTAATGA ACCATCACAG AGTGCATCCC CTGAGCCAGA GCCACCGCCA
    ACAAACAAGT GGCAACTGGA TAACTGGTTG AACAAAGTTA ATTCACATAA AGTGTCACCA
    GCTTCTTCCG TGGACAGCAA TATCCCATCC TCACAAGGCT ACAAAAAAGA GGGACGGGAT
    CAGGGAACAG GAAGTGGGTA CACTGATCAA AGTGGATCCA AGGACTCCAT TTCTTCAACA
    CCAGGGCGAG ATTCCAAACC CACCCAAAAG GGCTCAGAGA GCAGTCGTGG CAGGCAGAAA
    TCACCTGCCC AGAGTGACAG CTCAACACAG AGGAGGACTG TGGGTAAAAA GCAGCCCAAG
    AAGGCAGAAA AGACATCTGT TGAAGAGCCT AGAGGAGGTC TTAAAATAGA AAGTGAAACC
    CCAGTAGACA TGGCAACAAA CATACCTTCG AACAGGCATA AGGCAGCCAC TAAAGGTTCC
    AGAAAACCTA ATATAAAGAA AGAGCCCAAA TCTTCCCCTC GGCCTACAAC AGAGAAGAAG
    AAATACAAGG CTTCAAGCAA ATCTGCCCAG AAATCCAGGG AATTCATAGA AACAGATACC
    TCATCTTCTG ATTCAGATGA AAATGAAAGC CTGCCTCCTT CATCACAAAC ACCCAAATAC
    TCCGAGAGCA ATAGGACTCC TGTTAAATCT TCCATGGAGG AGGATGACAG CTTTTTTCGG
    CAAAGAATGT TCTCTCCTAT GGAAGAGAAA GAGCTTCTTT CACCACTCAG CGATCCTGAT
    GAAAGGTACC CACTTATTGT GAAGATTGAC CTGAGCCTCT TATCAAGAAT ACCAGGGAAG
    CCTTACAAGG ATGCTGATGT GCCCAAAGTG GAAAAGAAGA ATGTGCCAGA GAAGCATGCA
    AGAGAATCCC AGAAGCAGCC TTCTGATAAA AGTTCTACGA AAGGCAAAAG GAAACATAAA
    CAGAATGAGG ATGAAAACAG AGCCAGTGAA AGCAAGAAAA CGAAACTGGA AGAAAAAGCT
    CCATCAGGAC ACAAGACTTC TAGCAGCAGG GAGTCTTTAA AACCAAGTGC TGTTAAAGAG
    AAGGATTTAC TGCCATCTCC TGCTGCCCCA GTCCTTCAGA AGGATTCCAA ACAAGAGCAC
    GGGTCCCGGA AGAGGACAGT CAGTCAGTCG TCATCCTTGA AGTCCAGCAG CAATGTTAGT
    AAGGACAGCA GCAGTGGCAG TAAAAGCAGC TCATCTTCTA AACAGAAGAA GATGGAGGGC
    AAAGGTTCTG GCAGTGCTAA AGAAACAAAG GAAAAGATTC TGAACAATTC ATCGAACTGC
    CCTCCTGTTT CTGCTCCATC TACAGAGAGC TCAAAATCAA GAAGAGCAAA ACTTGTTTTT
    GATGACAGGA CTTACTCAGC TGATCATTAT TTACAGGAAG CAAAGAAGTT AAAACATAAT
    GCAGATGCAT TGTCTGACAG GTTTGAGAAG GCTGTGTACT ACCTAGATGC AGTAGTGTCG
    TTCATTGAGT GTGGGAATGC ATTGGAGAAA AATGCTCAGG AATCCAAGTC CCCGTTCCCT
    ATGTATTCAG AAACTGTGGA ATTAATCAAG TACACTATGA AACTGAAGAA TTACTTGGCA
    CCGGATGCTA CGGCTGCAGA CAAAAGGCTA GCAGTGCTTT GTCTTCGGTG CCAATCTCTA
    CTATACCTAA GGCTTTTCAA ACTGAAAAAG GAAAGTGCAT TGAAATATTC TAAAACTCTG
    ACTGAACATC TGAAGAATTC CTATAATAAT TCTCAAGCAC CGTCACCTGG TATGGGAAGC
    AAGCCTGTTG GTATGCCATC TCCAGTCTCT CCAAAGCTGT CTCCAGGGAA TTCAGGAAAT
    TACTCTTCTG GGACAGCCAA CCCTTCAGGG AGTGGGTCTT CGGTGATGAT CCCCCAGAGG
    ATCCATCAGA TGGCAGCCAG CTATGTCCAA GTTACATCCA ACTTCCTCTA TGCCACTGAA
    ATTTGGGACC AAGCTGAACA GCTGTCTAAA GAACAGAAAG AGTTCTTTGC TGAACTGGAT
    AAAGCTATGG GCCCTCTGGT TTTTAATTCA AGCATCATGA CAGACCTAGT GCGTTACACC
    CGTCAGGGAC TACACTGGTT GCGCCTGGAT GCCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42141
    Clone ID Related Accession (Same CDS sequence) XM_015294064.2 , XM_015294064.1
    Accession Version XM_015294064.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3459bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product AF4/FMR2 family member 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455962.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGAGAT GACCCATTCT TGGCCTCCTC CGCTGACTGC TATTCATACA
    CCATGCAAAA CTGAACCATC AAAATTTCCT TTTCCAACAA AGGAGTCTCA ACAGTCCAGT
    TTTGGCACTG GAGAACAGAA ACGATATAAT CCTTCATCAA AAACATCCAA TGGCCATCAG
    TCCAAATCAT GTGAATCGAA CCAGACAGAT ATGTTGAAAG ATGACTTAAA ACTTAGCAGT
    AGTGAAGACA GCGATGGAGA GCAGGACTGT GATAAGACAG TGCCAAGGAG CACACCTGGA
    AGTAGTTCTG AACCTTCACA GCATAACAGT GAAGGAGCAG ATAATTCAAG GGATGACTCG
    AGCAGCCACA GTGGGTCTGA AAGCAGTTCA GGATCAGACT CTGAAAGTGA AAGCAGTTCC
    AGTGATAGTG AAGCTAATGA ACCATCACAG AGTGCATCCC CTGAGCCAGA GCCACCGCCA
    ACAAACAAGT GGCAACTGGA TAACTGGTTG AACAAAGTTA ATTCACATAA AGTGTCACCA
    GCTTCTTCCG TGGACAGCAA TATCCCATCC TCACAAGGCT ACAAAAAAGA GGGACGGGAT
    CAGGGAACAG GAAGTGGGTA CACTGATCAA AGTGGATCCA AGGACTCCAT TTCTTCAACA
    CCAGGGCGAG ATTCCAAACC CACCCAAAAG GGCTCAGAGA GCAGTCGTGG CAGGCAGAAA
    TCACCTGCCC AGAGTGACAG CTCAACACAG AGGAGGACTG TGGGTAAAAA GCAGCCCAAG
    AAGGCAGAAA AGACATCTGT TGAAGAGCCT AGAGGAGGTC TTAAAATAGA AAGTGAAACC
    CCAGTAGACA TGGCAACAAA CATACCTTCG AACAGGCATA AGGCAGCCAC TAAAGGTTCC
    AGAAAACCTA ATATAAAGAA AGAGCCCAAA TCTTCCCCTC GGCCTACAAC AGAGAAGAAG
    AAATACAAGG CTTCAAGCAA ATCTGCCCAG AAATCCAGGG AATTCATAGA AACAGATACC
    TCATCTTCTG ATTCAGATGA AAATGAAAGC CTGCCTCCTT CATCACAAAC ACCCAAATAC
    TCCGAGAGCA ATAGGACTCC TGTTAAATCT TCCATGGAGG AGGATGACAG CTTTTTTCGG
    CAAAGAATGT TCTCTCCTAT GGAAGAGAAA GAGCTTCTTT CACCACTCAG CGATCCTGAT
    GAAAGGTACC CACTTATTGT GAAGATTGAC CTGAGCCTCT TATCAAGAAT ACCAGGGAAG
    CCTTACAAGG ATGCTGATGT GCCCAAAGTG GAAAAGAAGA ATGTGCCAGA GAAGCATGCA
    AGAGAATCCC AGAAGCAGCC TTCTGATAAA AGTTCTACGA AAGGCAAAAG GAAACATAAA
    CAGAATGAGG ATGAAAACAG AGCCAGTGAA AGCAAGAAAA CGAAACTGGA AGAAAAAGCT
    CCATCAGGAC ACAAGACTTC TAGCAGCAGG GAGTCTTTAA AACCAAGTGC TGTTAAAGAG
    AAGGATTTAC TGCCATCTCC TGCTGCCCCA GTCCTTCAGA AGGATTCCAA ACAAGAGCAC
    GGGTCCCGGA AGAGGACAGT CAGTCAGTCG TCATCCTTGA AGTCCAGCAG CAATGTTAGT
    AAGGACAGCA GCAGTGGCAG TAAAAGCAGC TCATCTTCTA AACAGAAGAA GATGGAGGGC
    AAAGGTTCTG GCAGTGCTAA AGAAACAAAG GAAAAGATTC TGAACAATTC ATCGAACTGC
    CCTCCTGTTT CTGCTCCATC TACAGAGAGC TCAAAATCAA GAAGAGCAAA ACTTGTTTTT
    GATGACAGGA CTTACTCAGC TGATCATTAT TTACAGGAAG CAAAGAAGTT AAAACATAAT
    GCAGATGCAT TGTCTGACAG GTTTGAGAAG GCTGTGTACT ACCTAGATGC AGTAGTGTCG
    TTCATTGAGT GTGGGAATGC ATTGGAGAAA AATGCTCAGG AATCCAAGTC CCCGTTCCCT
    ATGTATTCAG AAACTGTGGA ATTAATCAAG TACACTATGA AACTGAAGAA TTACTTGGCA
    CCGGATGCTA CGGCTGCAGA CAAAAGGCTA GCAGTGCTTT GTCTTCGGTG CCAATCTCTA
    CTATACCTAA GGCTTTTCAA ACTGAAAAAG GAAAGTGCAT TGAAATATTC TAAAACTCTG
    ACTGAACATC TGAAGAATTC CTATAATAAT TCTCAAGCAC CGTCACCTGG TATGGGAAGC
    AAGCCTGTTG GTATGCCATC TCCAGTCTCT CCAAAGCTGT CTCCAGGGAA TTCAGGAAAT
    TACTCTTCTG GGACAGCCAA CCCTTCAGGG AGTGGGTCTT CGGTGATGAT CCCCCAGAGG
    ATCCATCAGA TGGCAGCCAG CTATGTCCAA GTTACATCCA ACTTCCTCTA TGCCACTGAA
    ATTTGGGACC AAGCTGAACA GCTGTCTAAA GAACAGAAAG AGTTCTTTGC TGAACTGGAT
    AAAGCTATGG GCCCTCTGGT TTTTAATTCA AGCATCATGA CAGACCTAGT GCGTTACACC
    CGTCAGGGAC TACACTGGTT GCGCCTGGAT GCCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149550.1
    CDS640..4098
    Translation

    Target ORF information:

    RefSeq Version XM_015294064.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus AF4/FMR2 family, member 4 (AFF4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294064.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGAGAT GACCCATTCT TGGCCTCCTC CGCTGACTGC TATTCATACA
    CCATGCAAAA CTGAACCATC AAAATTTCCT TTTCCAACAA AGGAGTCTCA ACAGTCCAGT
    TTTGGCACTG GAGAACAGAA ACGATATAAT CCTTCATCAA AAACATCCAA TGGCCATCAG
    TCCAAATCAT GTGAATCGAA CCAGACAGAT ATGTTGAAAG ATGACTTAAA ACTTAGCAGT
    AGTGAAGACA GCGATGGAGA GCAGGACTGT GATAAGACAG TGCCAAGGAG CACACCTGGA
    AGTAGTTCTG AACCTTCACA GCATAACAGT GAAGGAGCAG ATAATTCAAG GGATGACTCG
    AGCAGCCACA GTGGGTCTGA AAGCAGTTCA GGATCAGACT CTGAAAGTGA AAGCAGTTCC
    AGTGATAGTG AAGCTAATGA ACCATCACAG AGTGCATCCC CTGAGCCAGA GCCACCGCCA
    ACAAACAAGT GGCAACTGGA TAACTGGTTG AACAAAGTTA ATTCACATAA AGTGTCACCA
    GCTTCTTCCG TGGACAGCAA TATCCCATCC TCACAAGGCT ACAAAAAAGA GGGACGGGAT
    CAGGGAACAG GAAGTGGGTA CACTGATCAA AGTGGATCCA AGGACTCCAT TTCTTCAACA
    CCAGGGCGAG ATTCCAAACC CACCCAAAAG GGCTCAGAGA GCAGTCGTGG CAGGCAGAAA
    TCACCTGCCC AGAGTGACAG CTCAACACAG AGGAGGACTG TGGGTAAAAA GCAGCCCAAG
    AAGGCAGAAA AGACATCTGT TGAAGAGCCT AGAGGAGGTC TTAAAATAGA AAGTGAAACC
    CCAGTAGACA TGGCAACAAA CATACCTTCG AACAGGCATA AGGCAGCCAC TAAAGGTTCC
    AGAAAACCTA ATATAAAGAA AGAGCCCAAA TCTTCCCCTC GGCCTACAAC AGAGAAGAAG
    AAATACAAGG CTTCAAGCAA ATCTGCCCAG AAATCCAGGG AATTCATAGA AACAGATACC
    TCATCTTCTG ATTCAGATGA AAATGAAAGC CTGCCTCCTT CATCACAAAC ACCCAAATAC
    TCCGAGAGCA ATAGGACTCC TGTTAAATCT TCCATGGAGG AGGATGACAG CTTTTTTCGG
    CAAAGAATGT TCTCTCCTAT GGAAGAGAAA GAGCTTCTTT CACCACTCAG CGATCCTGAT
    GAAAGGTACC CACTTATTGT GAAGATTGAC CTGAGCCTCT TATCAAGAAT ACCAGGGAAG
    CCTTACAAGG ATGCTGATGT GCCCAAAGTG GAAAAGAAGA ATGTGCCAGA GAAGCATGCA
    AGAGAATCCC AGAAGCAGCC TTCTGATAAA AGTTCTACGA AAGGCAAAAG GAAACATAAA
    CAGAATGAGG ATGAAAACAG AGCCAGTGAA AGCAAGAAAA CGAAACTGGA AGAAAAAGCT
    CCATCAGGAC ACAAGACTTC TAGCAGCAGG GAGTCTTTAA AACCAAGTGC TGTTAAAGAG
    AAGGATTTAC TGCCATCTCC TGCTGCCCCA GTCCTTCAGA AGGATTCCAA ACAAGAGCAC
    GGGTCCCGGA AGAGGACAGT CAGTCAGTCG TCATCCTTGA AGTCCAGCAG CAATGTTAGT
    AAGGACAGCA GCAGTGGCAG TAAAAGCAGC TCATCTTCTA AACAGAAGAA GATGGAGGGC
    AAAGGTTCTG GCAGTGCTAA AGAAACAAAG GAAAAGATTC TGAACAATTC ATCGAACTGC
    CCTCCTGTTT CTGCTCCATC TACAGAGAGC TCAAAATCAA GAAGAGCAAA ACTTGTTTTT
    GATGACAGGA CTTACTCAGC TGATCATTAT TTACAGGAAG CAAAGAAGTT AAAACATAAT
    GCAGATGCAT TGTCTGACAG GTTTGAGAAG GCTGTGTACT ACCTAGATGC AGTAGTGTCG
    TTCATTGAGT GTGGGAATGC ATTGGAGAAA AATGCTCAGG AATCCAAGTC CCCGTTCCCT
    ATGTATTCAG AAACTGTGGA ATTAATCAAG TACACTATGA AACTGAAGAA TTACTTGGCA
    CCGGATGCTA CGGCTGCAGA CAAAAGGCTA GCAGTGCTTT GTCTTCGGTG CCAATCTCTA
    CTATACCTAA GGCTTTTCAA ACTGAAAAAG GAAAGTGCAT TGAAATATTC TAAAACTCTG
    ACTGAACATC TGAAGAATTC CTATAATAAT TCTCAAGCAC CGTCACCTGG TATGGGAAGC
    AAGCCTGTTG GTATGCCATC TCCAGTCTCT CCAAAGCTGT CTCCAGGGAA TTCAGGAAAT
    TACTCTTCTG GGACAGCCAA CCCTTCAGGG AGTGGGTCTT CGGTGATGAT CCCCCAGAGG
    ATCCATCAGA TGGCAGCCAG CTATGTCCAA GTTACATCCA ACTTCCTCTA TGCCACTGAA
    ATTTGGGACC AAGCTGAACA GCTGTCTAAA GAACAGAAAG AGTTCTTTGC TGAACTGGAT
    AAAGCTATGG GCCCTCTGGT TTTTAATTCA AGCATCATGA CAGACCTAGT GCGTTACACC
    CGTCAGGGAC TACACTGGTT GCGCCTGGAT GCCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa14806
    Clone ID Related Accession (Same CDS sequence) XM_004945031.3 , XM_004945031.2 , XM_015294063.1 , XM_015294063.2 , XM_004945030.2 , XM_004945030.3
    Accession Version XM_004945031.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product AF4/FMR2 family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006100.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004945031.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGAGTTC TTTGCTGAAC TGGATAAAGC TATGGGCCCT
    CTGGTTTTTA ATTCAAGCAT CATGACAGAC CTAGTGCGTT ACACCCGTCA GGGACTACAC
    TGGTTGCGCC TGGATGCCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004945088.1
    CDS1330..4833
    Translation

    Target ORF information:

    RefSeq Version XM_004945031.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004945031.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGAGTTC TTTGCTGAAC TGGATAAAGC TATGGGCCCT
    CTGGTTTTTA ATTCAAGCAT CATGACAGAC CTAGTGCGTT ACACCCGTCA GGGACTACAC
    TGGTTGCGCC TGGATGCCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa14806
    Clone ID Related Accession (Same CDS sequence) XM_004945031.3 , XM_004945031.2 , XM_015294063.1 , XM_015294063.2 , XM_004945030.2 , XM_004945030.3
    Accession Version XM_004945031.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product AF4/FMR2 family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455962.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004945031.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGAGTTC TTTGCTGAAC TGGATAAAGC TATGGGCCCT
    CTGGTTTTTA ATTCAAGCAT CATGACAGAC CTAGTGCGTT ACACCCGTCA GGGACTACAC
    TGGTTGCGCC TGGATGCCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004945088.1
    CDS799..4302
    Translation

    Target ORF information:

    RefSeq Version XM_004945031.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus AF4/FMR2 family, member 4 (AFF4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004945031.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGAGTTC TTTGCTGAAC TGGATAAAGC TATGGGCCCT
    CTGGTTTTTA ATTCAAGCAT CATGACAGAC CTAGTGCGTT ACACCCGTCA GGGACTACAC
    TGGTTGCGCC TGGATGCCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa14806
    Clone ID Related Accession (Same CDS sequence) XM_004945031.3 , XM_004945031.2 , XM_015294063.1 , XM_015294063.2 , XM_004945030.2 , XM_004945030.3
    Accession Version XM_015294063.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product AF4/FMR2 family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455962.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGAGTTC TTTGCTGAAC TGGATAAAGC TATGGGCCCT
    CTGGTTTTTA ATTCAAGCAT CATGACAGAC CTAGTGCGTT ACACCCGTCA GGGACTACAC
    TGGTTGCGCC TGGATGCCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149549.1
    CDS142..3645
    Translation

    Target ORF information:

    RefSeq Version XM_015294063.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus AF4/FMR2 family, member 4 (AFF4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGAGTTC TTTGCTGAAC TGGATAAAGC TATGGGCCCT
    CTGGTTTTTA ATTCAAGCAT CATGACAGAC CTAGTGCGTT ACACCCGTCA GGGACTACAC
    TGGTTGCGCC TGGATGCCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa14806
    Clone ID Related Accession (Same CDS sequence) XM_004945031.3 , XM_004945031.2 , XM_015294063.1 , XM_015294063.2 , XM_004945030.2 , XM_004945030.3
    Accession Version XM_015294063.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product AF4/FMR2 family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006100.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294063.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGAGTTC TTTGCTGAAC TGGATAAAGC TATGGGCCCT
    CTGGTTTTTA ATTCAAGCAT CATGACAGAC CTAGTGCGTT ACACCCGTCA GGGACTACAC
    TGGTTGCGCC TGGATGCCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149549.1
    CDS139..3642
    Translation

    Target ORF information:

    RefSeq Version XM_015294063.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus AF4/FMR2 family member 4 (AFF4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294063.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGAGTTC TTTGCTGAAC TGGATAAAGC TATGGGCCCT
    CTGGTTTTTA ATTCAAGCAT CATGACAGAC CTAGTGCGTT ACACCCGTCA GGGACTACAC
    TGGTTGCGCC TGGATGCCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa14806
    Clone ID Related Accession (Same CDS sequence) XM_004945031.3 , XM_004945031.2 , XM_015294063.1 , XM_015294063.2 , XM_004945030.2 , XM_004945030.3
    Accession Version XM_004945030.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product AF4/FMR2 family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455962.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004945030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGAGTTC TTTGCTGAAC TGGATAAAGC TATGGGCCCT
    CTGGTTTTTA ATTCAAGCAT CATGACAGAC CTAGTGCGTT ACACCCGTCA GGGACTACAC
    TGGTTGCGCC TGGATGCCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004945087.1
    CDS644..4147
    Translation

    Target ORF information:

    RefSeq Version XM_004945030.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus AF4/FMR2 family, member 4 (AFF4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004945030.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGAGTTC TTTGCTGAAC TGGATAAAGC TATGGGCCCT
    CTGGTTTTTA ATTCAAGCAT CATGACAGAC CTAGTGCGTT ACACCCGTCA GGGACTACAC
    TGGTTGCGCC TGGATGCCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa14806
    Clone ID Related Accession (Same CDS sequence) XM_004945031.3 , XM_004945031.2 , XM_015294063.1 , XM_015294063.2 , XM_004945030.2 , XM_004945030.3
    Accession Version XM_004945030.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product AF4/FMR2 family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006100.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004945030.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGAGTTC TTTGCTGAAC TGGATAAAGC TATGGGCCCT
    CTGGTTTTTA ATTCAAGCAT CATGACAGAC CTAGTGCGTT ACACCCGTCA GGGACTACAC
    TGGTTGCGCC TGGATGCCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004945087.1
    CDS1176..4679
    Translation

    Target ORF information:

    RefSeq Version XM_004945030.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004945030.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGAGTTC TTTGCTGAAC TGGATAAAGC TATGGGCCCT
    CTGGTTTTTA ATTCAAGCAT CATGACAGAC CTAGTGCGTT ACACCCGTCA GGGACTACAC
    TGGTTGCGCC TGGATGCCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa14807
    Clone ID Related Accession (Same CDS sequence) XM_004945032.2 , XM_004945032.3
    Accession Version XM_004945032.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product AF4/FMR2 family member 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455962.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004945032.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTTC TGAACCTTCA
    CAGCATAACA GTGAAGGAGC AGATAATTCA AGGGATGACT CGAGCAGCCA CAGTGGGTCT
    GAAAGCAGTT CAGGATCAGA CTCTGAAAGT GAAAGCAGTT CCAGTGATAG TGAAGCTAAT
    GAACCATCAC AGAGTGCATC CCCTGAGCCA GAGCCACCGC CAACAAACAA GTGGCAACTG
    GATAACTGGT TGAACAAAGT TAATTCACAT AAAGTGTCAC CAGCTTCTTC CGTGGACAGC
    AATATCCCAT CCTCACAAGG CTACAAAAAA GAGGGACGGG ATCAGGGAAC AGGAAGTGGG
    TACACTGATC AAAGTGGATC CAAGGACTCC ATTTCTTCAA CACCAGGGCG AGATTCCAAA
    CCCACCCAAA AGGGCTCAGA GAGCAGTCGT GGCAGGCAGA AATCACCTGC CCAGAGTGAC
    AGCTCAACAC AGAGGAGGAC TGTGGGTAAA AAGCAGCCCA AGAAGGCAGA AAAGACATCT
    GTTGAAGAGC CTAGAGGAGG TCTTAAAATA GAAAGTGAAA CCCCAGTAGA CATGGCAACA
    AACATACCTT CGAACAGGCA TAAGGCAGCC ACTAAAGGTT CCAGAAAACC TAATATAAAG
    AAAGAGCCCA AATCTTCCCC TCGGCCTACA ACAGAGAAGA AGAAATACAA GGCTTCAAGC
    AAATCTGCCC AGAAATCCAG GGAATTCATA GAAACAGATA CCTCATCTTC TGATTCAGAT
    GAAAATGAAA GCCTGCCTCC TTCATCACAA ACACCCAAAT ACTCCGAGAG CAATAGGACT
    CCTGTTAAAT CTTCCATGGA GGAGGATGAC AGCTTTTTTC GGCAAAGAAT GTTCTCTCCT
    ATGGAAGAGA AAGAGCTTCT TTCACCACTC AGCGATCCTG ATGAAAGGTA CCCACTTATT
    GTGAAGATTG ACCTGAGCCT CTTATCAAGA ATACCAGGGA AGCCTTACAA GGATGCTGAT
    GTGCCCAAAG TGGAAAAGAA GAATGTGCCA GAGAAGCATG CAAGAGAATC CCAGAAGCAG
    CCTTCTGATA AAAGTTCTAC GAAAGGCAAA AGGAAACATA AACAGAATGA GGATGAAAAC
    AGAGCCAGTG AAAGCAAGAA AACGAAACTG GAAGAAAAAG CTCCATCAGG ACACAAGACT
    TCTAGCAGCA GGGAGTCTTT AAAACCAAGT GCTGTTAAAG AGAAGGATTT ACTGCCATCT
    CCTGCTGCCC CAGTCCTTCA GAAGGATTCC AAACAAGAGC ACGGGTCCCG GAAGAGGACA
    GTCAGTCAGT CGTCATCCTT GAAGTCCAGC AGCAATGTTA GTAAGGACAG CAGCAGTGGC
    AGTAAAAGCA GCTCATCTTC TAAACAGAAG AAGATGGAGG GCAAAGGTTC TGGCAGTGCT
    AAAGAAACAA AGGAAAAGAT TCTGAACAAT TCATCGAACT GCCCTCCTGT TTCTGCTCCA
    TCTACAGAGA GCTCAAAATC AAGAAGAGCA AAACTTGTTT TTGATGACAG GACTTACTCA
    GCTGATCATT ATTTACAGGA AGCAAAGAAG TTAAAACATA ATGCAGATGC ATTGTCTGAC
    AGGTTTGAGA AGGCTGTGTA CTACCTAGAT GCAGTAGTGT CGTTCATTGA GTGTGGGAAT
    GCATTGGAGA AAAATGCTCA GGAATCCAAG TCCCCGTTCC CTATGTATTC AGAAACTGTG
    GAATTAATCA AGTACACTAT GAAACTGAAG AATTACTTGG CACCGGATGC TACGGCTGCA
    GACAAAAGGC TAGCAGTGCT TTGTCTTCGG TGCCAATCTC TACTATACCT AAGGCTTTTC
    AAACTGAAAA AGGAAAGTGC ATTGAAATAT TCTAAAACTC TGACTGAACA TCTGAAGAAT
    TCCTATAATA ATTCTCAAGC ACCGTCACCT GGTATGGGAA GCAAGCCTGT TGGTATGCCA
    TCTCCAGTCT CTCCAAAGCT GTCTCCAGGG AATTCAGGAA ATTACTCTTC TGGGACAGCC
    AACCCTTCAG GGAGTGGGTC TTCGGTGATG ATCCCCCAGA GGATCCATCA GATGGCAGCC
    AGCTATGTCC AAGTTACATC CAACTTCCTC TATGCCACTG AAATTTGGGA CCAAGCTGAA
    CAGCTGTCTA AAGAACAGAA AGAGTTCTTT GCTGAACTGG ATAAAGCTAT GGGCCCTCTG
    GTTTTTAATT CAAGCATCAT GACAGACCTA GTGCGTTACA CCCGTCAGGG ACTACACTGG
    TTGCGCCTGG ATGCCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004945089.1
    CDS645..4145
    Translation

    Target ORF information:

    RefSeq Version XM_004945032.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus AF4/FMR2 family, member 4 (AFF4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004945032.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTTC TGAACCTTCA
    CAGCATAACA GTGAAGGAGC AGATAATTCA AGGGATGACT CGAGCAGCCA CAGTGGGTCT
    GAAAGCAGTT CAGGATCAGA CTCTGAAAGT GAAAGCAGTT CCAGTGATAG TGAAGCTAAT
    GAACCATCAC AGAGTGCATC CCCTGAGCCA GAGCCACCGC CAACAAACAA GTGGCAACTG
    GATAACTGGT TGAACAAAGT TAATTCACAT AAAGTGTCAC CAGCTTCTTC CGTGGACAGC
    AATATCCCAT CCTCACAAGG CTACAAAAAA GAGGGACGGG ATCAGGGAAC AGGAAGTGGG
    TACACTGATC AAAGTGGATC CAAGGACTCC ATTTCTTCAA CACCAGGGCG AGATTCCAAA
    CCCACCCAAA AGGGCTCAGA GAGCAGTCGT GGCAGGCAGA AATCACCTGC CCAGAGTGAC
    AGCTCAACAC AGAGGAGGAC TGTGGGTAAA AAGCAGCCCA AGAAGGCAGA AAAGACATCT
    GTTGAAGAGC CTAGAGGAGG TCTTAAAATA GAAAGTGAAA CCCCAGTAGA CATGGCAACA
    AACATACCTT CGAACAGGCA TAAGGCAGCC ACTAAAGGTT CCAGAAAACC TAATATAAAG
    AAAGAGCCCA AATCTTCCCC TCGGCCTACA ACAGAGAAGA AGAAATACAA GGCTTCAAGC
    AAATCTGCCC AGAAATCCAG GGAATTCATA GAAACAGATA CCTCATCTTC TGATTCAGAT
    GAAAATGAAA GCCTGCCTCC TTCATCACAA ACACCCAAAT ACTCCGAGAG CAATAGGACT
    CCTGTTAAAT CTTCCATGGA GGAGGATGAC AGCTTTTTTC GGCAAAGAAT GTTCTCTCCT
    ATGGAAGAGA AAGAGCTTCT TTCACCACTC AGCGATCCTG ATGAAAGGTA CCCACTTATT
    GTGAAGATTG ACCTGAGCCT CTTATCAAGA ATACCAGGGA AGCCTTACAA GGATGCTGAT
    GTGCCCAAAG TGGAAAAGAA GAATGTGCCA GAGAAGCATG CAAGAGAATC CCAGAAGCAG
    CCTTCTGATA AAAGTTCTAC GAAAGGCAAA AGGAAACATA AACAGAATGA GGATGAAAAC
    AGAGCCAGTG AAAGCAAGAA AACGAAACTG GAAGAAAAAG CTCCATCAGG ACACAAGACT
    TCTAGCAGCA GGGAGTCTTT AAAACCAAGT GCTGTTAAAG AGAAGGATTT ACTGCCATCT
    CCTGCTGCCC CAGTCCTTCA GAAGGATTCC AAACAAGAGC ACGGGTCCCG GAAGAGGACA
    GTCAGTCAGT CGTCATCCTT GAAGTCCAGC AGCAATGTTA GTAAGGACAG CAGCAGTGGC
    AGTAAAAGCA GCTCATCTTC TAAACAGAAG AAGATGGAGG GCAAAGGTTC TGGCAGTGCT
    AAAGAAACAA AGGAAAAGAT TCTGAACAAT TCATCGAACT GCCCTCCTGT TTCTGCTCCA
    TCTACAGAGA GCTCAAAATC AAGAAGAGCA AAACTTGTTT TTGATGACAG GACTTACTCA
    GCTGATCATT ATTTACAGGA AGCAAAGAAG TTAAAACATA ATGCAGATGC ATTGTCTGAC
    AGGTTTGAGA AGGCTGTGTA CTACCTAGAT GCAGTAGTGT CGTTCATTGA GTGTGGGAAT
    GCATTGGAGA AAAATGCTCA GGAATCCAAG TCCCCGTTCC CTATGTATTC AGAAACTGTG
    GAATTAATCA AGTACACTAT GAAACTGAAG AATTACTTGG CACCGGATGC TACGGCTGCA
    GACAAAAGGC TAGCAGTGCT TTGTCTTCGG TGCCAATCTC TACTATACCT AAGGCTTTTC
    AAACTGAAAA AGGAAAGTGC ATTGAAATAT TCTAAAACTC TGACTGAACA TCTGAAGAAT
    TCCTATAATA ATTCTCAAGC ACCGTCACCT GGTATGGGAA GCAAGCCTGT TGGTATGCCA
    TCTCCAGTCT CTCCAAAGCT GTCTCCAGGG AATTCAGGAA ATTACTCTTC TGGGACAGCC
    AACCCTTCAG GGAGTGGGTC TTCGGTGATG ATCCCCCAGA GGATCCATCA GATGGCAGCC
    AGCTATGTCC AAGTTACATC CAACTTCCTC TATGCCACTG AAATTTGGGA CCAAGCTGAA
    CAGCTGTCTA AAGAACAGAA AGAGTTCTTT GCTGAACTGG ATAAAGCTAT GGGCCCTCTG
    GTTTTTAATT CAAGCATCAT GACAGACCTA GTGCGTTACA CCCGTCAGGG ACTACACTGG
    TTGCGCCTGG ATGCCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa14807
    Clone ID Related Accession (Same CDS sequence) XM_004945032.2 , XM_004945032.3
    Accession Version XM_004945032.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product AF4/FMR2 family member 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006100.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004945032.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTTC TGAACCTTCA
    CAGCATAACA GTGAAGGAGC AGATAATTCA AGGGATGACT CGAGCAGCCA CAGTGGGTCT
    GAAAGCAGTT CAGGATCAGA CTCTGAAAGT GAAAGCAGTT CCAGTGATAG TGAAGCTAAT
    GAACCATCAC AGAGTGCATC CCCTGAGCCA GAGCCACCGC CAACAAACAA GTGGCAACTG
    GATAACTGGT TGAACAAAGT TAATTCACAT AAAGTGTCAC CAGCTTCTTC CGTGGACAGC
    AATATCCCAT CCTCACAAGG CTACAAAAAA GAGGGACGGG ATCAGGGAAC AGGAAGTGGG
    TACACTGATC AAAGTGGATC CAAGGACTCC ATTTCTTCAA CACCAGGGCG AGATTCCAAA
    CCCACCCAAA AGGGCTCAGA GAGCAGTCGT GGCAGGCAGA AATCACCTGC CCAGAGTGAC
    AGCTCAACAC AGAGGAGGAC TGTGGGTAAA AAGCAGCCCA AGAAGGCAGA AAAGACATCT
    GTTGAAGAGC CTAGAGGAGG TCTTAAAATA GAAAGTGAAA CCCCAGTAGA CATGGCAACA
    AACATACCTT CGAACAGGCA TAAGGCAGCC ACTAAAGGTT CCAGAAAACC TAATATAAAG
    AAAGAGCCCA AATCTTCCCC TCGGCCTACA ACAGAGAAGA AGAAATACAA GGCTTCAAGC
    AAATCTGCCC AGAAATCCAG GGAATTCATA GAAACAGATA CCTCATCTTC TGATTCAGAT
    GAAAATGAAA GCCTGCCTCC TTCATCACAA ACACCCAAAT ACTCCGAGAG CAATAGGACT
    CCTGTTAAAT CTTCCATGGA GGAGGATGAC AGCTTTTTTC GGCAAAGAAT GTTCTCTCCT
    ATGGAAGAGA AAGAGCTTCT TTCACCACTC AGCGATCCTG ATGAAAGGTA CCCACTTATT
    GTGAAGATTG ACCTGAGCCT CTTATCAAGA ATACCAGGGA AGCCTTACAA GGATGCTGAT
    GTGCCCAAAG TGGAAAAGAA GAATGTGCCA GAGAAGCATG CAAGAGAATC CCAGAAGCAG
    CCTTCTGATA AAAGTTCTAC GAAAGGCAAA AGGAAACATA AACAGAATGA GGATGAAAAC
    AGAGCCAGTG AAAGCAAGAA AACGAAACTG GAAGAAAAAG CTCCATCAGG ACACAAGACT
    TCTAGCAGCA GGGAGTCTTT AAAACCAAGT GCTGTTAAAG AGAAGGATTT ACTGCCATCT
    CCTGCTGCCC CAGTCCTTCA GAAGGATTCC AAACAAGAGC ACGGGTCCCG GAAGAGGACA
    GTCAGTCAGT CGTCATCCTT GAAGTCCAGC AGCAATGTTA GTAAGGACAG CAGCAGTGGC
    AGTAAAAGCA GCTCATCTTC TAAACAGAAG AAGATGGAGG GCAAAGGTTC TGGCAGTGCT
    AAAGAAACAA AGGAAAAGAT TCTGAACAAT TCATCGAACT GCCCTCCTGT TTCTGCTCCA
    TCTACAGAGA GCTCAAAATC AAGAAGAGCA AAACTTGTTT TTGATGACAG GACTTACTCA
    GCTGATCATT ATTTACAGGA AGCAAAGAAG TTAAAACATA ATGCAGATGC ATTGTCTGAC
    AGGTTTGAGA AGGCTGTGTA CTACCTAGAT GCAGTAGTGT CGTTCATTGA GTGTGGGAAT
    GCATTGGAGA AAAATGCTCA GGAATCCAAG TCCCCGTTCC CTATGTATTC AGAAACTGTG
    GAATTAATCA AGTACACTAT GAAACTGAAG AATTACTTGG CACCGGATGC TACGGCTGCA
    GACAAAAGGC TAGCAGTGCT TTGTCTTCGG TGCCAATCTC TACTATACCT AAGGCTTTTC
    AAACTGAAAA AGGAAAGTGC ATTGAAATAT TCTAAAACTC TGACTGAACA TCTGAAGAAT
    TCCTATAATA ATTCTCAAGC ACCGTCACCT GGTATGGGAA GCAAGCCTGT TGGTATGCCA
    TCTCCAGTCT CTCCAAAGCT GTCTCCAGGG AATTCAGGAA ATTACTCTTC TGGGACAGCC
    AACCCTTCAG GGAGTGGGTC TTCGGTGATG ATCCCCCAGA GGATCCATCA GATGGCAGCC
    AGCTATGTCC AAGTTACATC CAACTTCCTC TATGCCACTG AAATTTGGGA CCAAGCTGAA
    CAGCTGTCTA AAGAACAGAA AGAGTTCTTT GCTGAACTGG ATAAAGCTAT GGGCCCTCTG
    GTTTTTAATT CAAGCATCAT GACAGACCTA GTGCGTTACA CCCGTCAGGG ACTACACTGG
    TTGCGCCTGG ATGCCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004945089.1
    CDS1176..4676
    Translation

    Target ORF information:

    RefSeq Version XM_004945032.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus AF4/FMR2 family member 4 (AFF4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004945032.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTTC TGAACCTTCA
    CAGCATAACA GTGAAGGAGC AGATAATTCA AGGGATGACT CGAGCAGCCA CAGTGGGTCT
    GAAAGCAGTT CAGGATCAGA CTCTGAAAGT GAAAGCAGTT CCAGTGATAG TGAAGCTAAT
    GAACCATCAC AGAGTGCATC CCCTGAGCCA GAGCCACCGC CAACAAACAA GTGGCAACTG
    GATAACTGGT TGAACAAAGT TAATTCACAT AAAGTGTCAC CAGCTTCTTC CGTGGACAGC
    AATATCCCAT CCTCACAAGG CTACAAAAAA GAGGGACGGG ATCAGGGAAC AGGAAGTGGG
    TACACTGATC AAAGTGGATC CAAGGACTCC ATTTCTTCAA CACCAGGGCG AGATTCCAAA
    CCCACCCAAA AGGGCTCAGA GAGCAGTCGT GGCAGGCAGA AATCACCTGC CCAGAGTGAC
    AGCTCAACAC AGAGGAGGAC TGTGGGTAAA AAGCAGCCCA AGAAGGCAGA AAAGACATCT
    GTTGAAGAGC CTAGAGGAGG TCTTAAAATA GAAAGTGAAA CCCCAGTAGA CATGGCAACA
    AACATACCTT CGAACAGGCA TAAGGCAGCC ACTAAAGGTT CCAGAAAACC TAATATAAAG
    AAAGAGCCCA AATCTTCCCC TCGGCCTACA ACAGAGAAGA AGAAATACAA GGCTTCAAGC
    AAATCTGCCC AGAAATCCAG GGAATTCATA GAAACAGATA CCTCATCTTC TGATTCAGAT
    GAAAATGAAA GCCTGCCTCC TTCATCACAA ACACCCAAAT ACTCCGAGAG CAATAGGACT
    CCTGTTAAAT CTTCCATGGA GGAGGATGAC AGCTTTTTTC GGCAAAGAAT GTTCTCTCCT
    ATGGAAGAGA AAGAGCTTCT TTCACCACTC AGCGATCCTG ATGAAAGGTA CCCACTTATT
    GTGAAGATTG ACCTGAGCCT CTTATCAAGA ATACCAGGGA AGCCTTACAA GGATGCTGAT
    GTGCCCAAAG TGGAAAAGAA GAATGTGCCA GAGAAGCATG CAAGAGAATC CCAGAAGCAG
    CCTTCTGATA AAAGTTCTAC GAAAGGCAAA AGGAAACATA AACAGAATGA GGATGAAAAC
    AGAGCCAGTG AAAGCAAGAA AACGAAACTG GAAGAAAAAG CTCCATCAGG ACACAAGACT
    TCTAGCAGCA GGGAGTCTTT AAAACCAAGT GCTGTTAAAG AGAAGGATTT ACTGCCATCT
    CCTGCTGCCC CAGTCCTTCA GAAGGATTCC AAACAAGAGC ACGGGTCCCG GAAGAGGACA
    GTCAGTCAGT CGTCATCCTT GAAGTCCAGC AGCAATGTTA GTAAGGACAG CAGCAGTGGC
    AGTAAAAGCA GCTCATCTTC TAAACAGAAG AAGATGGAGG GCAAAGGTTC TGGCAGTGCT
    AAAGAAACAA AGGAAAAGAT TCTGAACAAT TCATCGAACT GCCCTCCTGT TTCTGCTCCA
    TCTACAGAGA GCTCAAAATC AAGAAGAGCA AAACTTGTTT TTGATGACAG GACTTACTCA
    GCTGATCATT ATTTACAGGA AGCAAAGAAG TTAAAACATA ATGCAGATGC ATTGTCTGAC
    AGGTTTGAGA AGGCTGTGTA CTACCTAGAT GCAGTAGTGT CGTTCATTGA GTGTGGGAAT
    GCATTGGAGA AAAATGCTCA GGAATCCAAG TCCCCGTTCC CTATGTATTC AGAAACTGTG
    GAATTAATCA AGTACACTAT GAAACTGAAG AATTACTTGG CACCGGATGC TACGGCTGCA
    GACAAAAGGC TAGCAGTGCT TTGTCTTCGG TGCCAATCTC TACTATACCT AAGGCTTTTC
    AAACTGAAAA AGGAAAGTGC ATTGAAATAT TCTAAAACTC TGACTGAACA TCTGAAGAAT
    TCCTATAATA ATTCTCAAGC ACCGTCACCT GGTATGGGAA GCAAGCCTGT TGGTATGCCA
    TCTCCAGTCT CTCCAAAGCT GTCTCCAGGG AATTCAGGAA ATTACTCTTC TGGGACAGCC
    AACCCTTCAG GGAGTGGGTC TTCGGTGATG ATCCCCCAGA GGATCCATCA GATGGCAGCC
    AGCTATGTCC AAGTTACATC CAACTTCCTC TATGCCACTG AAATTTGGGA CCAAGCTGAA
    CAGCTGTCTA AAGAACAGAA AGAGTTCTTT GCTGAACTGG ATAAAGCTAT GGGCCCTCTG
    GTTTTTAATT CAAGCATCAT GACAGACCTA GTGCGTTACA CCCGTCAGGG ACTACACTGG
    TTGCGCCTGG ATGCCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa14809
    Clone ID Related Accession (Same CDS sequence) XM_004945033.2 , XM_004945033.3
    Accession Version XM_004945033.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product AF4/FMR2 family member 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455962.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004945033.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGGCCTT TATTTGAAGT ACGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004945090.1
    CDS647..4054
    Translation

    Target ORF information:

    RefSeq Version XM_004945033.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus AF4/FMR2 family, member 4 (AFF4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004945033.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGGCCTT TATTTGAAGT ACGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa14809
    Clone ID Related Accession (Same CDS sequence) XM_004945033.2 , XM_004945033.3
    Accession Version XM_004945033.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product AF4/FMR2 family member 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006100.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004945033.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGGCCTT TATTTGAAGT ACGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004945090.1
    CDS1178..4585
    Translation

    Target ORF information:

    RefSeq Version XM_004945033.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus AF4/FMR2 family member 4 (AFF4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004945033.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCGTG AAGACCGAAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAAGAA 
    ATCCAGCAGG GTGAAGAAGC CTTTCCGCCC AACTCTCCTC TCTTTGCTGA ACCATACAAA
    GTTACCAGCA AAGAAGACAA ATTATCTAGT CGCATTCAGA GCATGCTTGG TAATTATGAT
    GAAATGGATG ATCTTATAGG AGATAGATCG CTACCAAAGC TTGTTGGAAT TCCCAAACCT
    ACTGTACCAT CAACAGCAGA TGAAAAATCA AACCAAAGTT TCTTTGGTGA TCAGAGACAT
    AATGCTAGTT CCCATCAGAG TAGCAAATGG ACTCCTGTAG GACCAGCACC TAGCACTTCC
    TCACAGTCTC AGAAACGGTC CTCGGGTTTA CAGAGCGGCC ACAGCGGTCA GCGTGGCGGT
    GGCAATAACA CTAGCAGTGG TGGCCAGAGG CACGACCGTG ACTCGTACAG TAGCAGCAGC
    AGTCGCAAAA AAAGCCAGCA TGGATCAGAA CACTCCAAAT CCCGTTCTTC CAGCCCTGGG
    AAACCACCTG CTGTTTCTTC GTTAAGCTCC AGCCATTCGA GATCTCATGG AAGTGATCAT
    CACAGCAAAG AACATCAACG CTCAAAGTCT CCCCGGGATC CGGATGCCAA CTGGGACTCT
    CCTTCGCGTG TACCCTCATT TTCAAGTGGA CAGCACTCTA ACCAGTCTTT TCCACCTTCG
    CTCATGTCCA AGTCCAGTTC AATGTTACAG AAACCCACTG CTTATGTAAG GCCAATGGAT
    GGACAGGAAT CCATGGAACC AAAGTTATCT TCCGAACACT ACAGTAGTCA GACCCACAGC
    AGCGGTGTGA ACGAGCTGAA GCCTAGCAGC AAGGCACATC TAACCAAGCT GAAAATACCT
    TCTCAACCAC TCGATGCATC AGCATCTGGT GATGTGAGCT GCGTGGATGA AATTCTAAAA
    GAGATGACCC ATTCTTGGCC TCCTCCGCTG ACTGCTATTC ATACACCATG CAAAACTGAA
    CCATCAAAAT TTCCTTTTCC AACAAAGGAG TCTCAACAGT CCAGTTTTGG CACTGGAGAA
    CAGAAACGAT ATAATCCTTC ATCAAAAACA TCCAATGGCC ATCAGTCCAA ATCATGTGAA
    TCGAACCAGA CAGATATGTT GAAAGATGAC TTAAAACTTA GCAGTAGTGA AGACAGCGAT
    GGAGAGCAGG ACTGTGATAA GACAGTGCCA AGGAGCACAC CTGGAAGTAG TTCTGAACCT
    TCACAGCATA ACAGTGAAGG AGCAGATAAT TCAAGGGATG ACTCGAGCAG CCACAGTGGG
    TCTGAAAGCA GTTCAGGATC AGACTCTGAA AGTGAAAGCA GTTCCAGTGA TAGTGAAGCT
    AATGAACCAT CACAGAGTGC ATCCCCTGAG CCAGAGCCAC CGCCAACAAA CAAGTGGCAA
    CTGGATAACT GGTTGAACAA AGTTAATTCA CATAAAGTGT CACCAGCTTC TTCCGTGGAC
    AGCAATATCC CATCCTCACA AGGCTACAAA AAAGAGGGAC GGGATCAGGG AACAGGAAGT
    GGGTACACTG ATCAAAGTGG ATCCAAGGAC TCCATTTCTT CAACACCAGG GCGAGATTCC
    AAACCCACCC AAAAGGGCTC AGAGAGCAGT CGTGGCAGGC AGAAATCACC TGCCCAGAGT
    GACAGCTCAA CACAGAGGAG GACTGTGGGT AAAAAGCAGC CCAAGAAGGC AGAAAAGACA
    TCTGTTGAAG AGCCTAGAGG AGGTCTTAAA ATAGAAAGTG AAACCCCAGT AGACATGGCA
    ACAAACATAC CTTCGAACAG GCATAAGGCA GCCACTAAAG GTTCCAGAAA ACCTAATATA
    AAGAAAGAGC CCAAATCTTC CCCTCGGCCT ACAACAGAGA AGAAGAAATA CAAGGCTTCA
    AGCAAATCTG CCCAGAAATC CAGGGAATTC ATAGAAACAG ATACCTCATC TTCTGATTCA
    GATGAAAATG AAAGCCTGCC TCCTTCATCA CAAACACCCA AATACTCCGA GAGCAATAGG
    ACTCCTGTTA AATCTTCCAT GGAGGAGGAT GACAGCTTTT TTCGGCAAAG AATGTTCTCT
    CCTATGGAAG AGAAAGAGCT TCTTTCACCA CTCAGCGATC CTGATGAAAG GTACCCACTT
    ATTGTGAAGA TTGACCTGAG CCTCTTATCA AGAATACCAG GGAAGCCTTA CAAGGATGCT
    GATGTGCCCA AAGTGGAAAA GAAGAATGTG CCAGAGAAGC ATGCAAGAGA ATCCCAGAAG
    CAGCCTTCTG ATAAAAGTTC TACGAAAGGC AAAAGGAAAC ATAAACAGAA TGAGGATGAA
    AACAGAGCCA GTGAAAGCAA GAAAACGAAA CTGGAAGAAA AAGCTCCATC AGGACACAAG
    ACTTCTAGCA GCAGGGAGTC TTTAAAACCA AGTGCTGTTA AAGAGAAGGA TTTACTGCCA
    TCTCCTGCTG CCCCAGTCCT TCAGAAGGAT TCCAAACAAG AGCACGGGTC CCGGAAGAGG
    ACAGTCAGTC AGTCGTCATC CTTGAAGTCC AGCAGCAATG TTAGTAAGGA CAGCAGCAGT
    GGCAGTAAAA GCAGCTCATC TTCTAAACAG AAGAAGATGG AGGGCAAAGG TTCTGGCAGT
    GCTAAAGAAA CAAAGGAAAA GATTCTGAAC AATTCATCGA ACTGCCCTCC TGTTTCTGCT
    CCATCTACAG AGAGCTCAAA ATCAAGAAGA GCAAAACTTG TTTTTGATGA CAGGACTTAC
    TCAGCTGATC ATTATTTACA GGAAGCAAAG AAGTTAAAAC ATAATGCAGA TGCATTGTCT
    GACAGGTTTG AGAAGGCTGT GTACTACCTA GATGCAGTAG TGTCGTTCAT TGAGTGTGGG
    AATGCATTGG AGAAAAATGC TCAGGAATCC AAGTCCCCGT TCCCTATGTA TTCAGAAACT
    GTGGAATTAA TCAAGTACAC TATGAAACTG AAGAATTACT TGGCACCGGA TGCTACGGCT
    GCAGACAAAA GGCTAGCAGT GCTTTGTCTT CGGTGCCAAT CTCTACTATA CCTAAGGCTT
    TTCAAACTGA AAAAGGAAAG TGCATTGAAA TATTCTAAAA CTCTGACTGA ACATCTGAAG
    AATTCCTATA ATAATTCTCA AGCACCGTCA CCTGGTATGG GAAGCAAGCC TGTTGGTATG
    CCATCTCCAG TCTCTCCAAA GCTGTCTCCA GGGAATTCAG GAAATTACTC TTCTGGGACA
    GCCAACCCTT CAGGGAGTGG GTCTTCGGTG ATGATCCCCC AGAGGATCCA TCAGATGGCA
    GCCAGCTATG TCCAAGTTAC ATCCAACTTC CTCTATGCCA CTGAAATTTG GGACCAAGCT
    GAACAGCTGT CTAAAGAACA GAAAGGCCTT TATTTGAAGT ACGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.